LOCUS       DASYGU010000001         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1080309, whole genome shotgun sequence.
ACCESSION   DASYGU010000001 DASYGU010000000
VERSION     DASYGU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080309"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VGO32_00005"
     rRNA            <1..912
                     /locus_tag="VGO32_00005"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1020..1136
                     /gene="rrf"
                     /locus_tag="VGO32_00010"
     rRNA            1020..1136
                     /gene="rrf"
                     /locus_tag="VGO32_00010"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1214..1888
                     /locus_tag="VGO32_00015"
     CDS             1214..1888
                     /locus_tag="VGO32_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV7199171.1"
                     /translation="MDPICPLLSLGGDPRVVSATPDDAHRCLADGTPAPIEREYQVRF
                     CLTARHETCERYLAHLADVGPVGPTWRPAAPDSAYGSTRLVMESAPRSVIRRRPRRFG
                     MAALFVIGLVLVGVLVAWIGLGSILAPPAGSPTPSATASATPEESASPSPSPSPTPEP
                     TPTPIPTPTPVPAPTPISYIVQSGDTLNEIASRFGTTAQAIADFNGINVADPIQVGQV
                     LLIPVS"
     gene            complement(1912..2733)
                     /locus_tag="VGO32_00020"
     CDS             complement(1912..2733)
                     /locus_tag="VGO32_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEV7199172.1"
                     /translation="MPELPDLTIVAEELERRATGREIAAATAPTPILLRATAADLESL
                     TGMTLGPAGRRGKFLILPLLRAGLEERLLLANPMLAGRFWLIDAPGQKVRARTGLRLT
                     FTDGAELRYVDREMLGKLYLVDRDRLDDVPGWADMGPDADDPELTLEAFRARIRRHPG
                     ELKSLLRNQRFVAGIGNAYSDEILWAARLAPLRKRRSLAPEDIDRLYEAMRAVLADAT
                     VCLRDLVPPHIETQHREFLQVHLRGGEPCPRCGRPLRQIGGDEATTFCRTCQPPF"
     gene            2944..5049
                     /locus_tag="VGO32_00025"
     CDS             2944..5049
                     /locus_tag="VGO32_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV7199173.1"
                     /translation="MDEDPQPAAPTDEPSSAGPDAAMTLSEEPTSSPTEAATMVADPE
                     PDPTAEALDELAVEARASADVAEAVAAVEVAEAIEAVAVAEELAEVADAVESVAMDEV
                     TADEPVADEPVAEVTAEGEPAAEVPVEDERPEPTTMAEFLDHPDHEVKNLRHGDVVEG
                     VVVRIDPDELLVDFGGKSEGVVSNRELLGRRGGPREGDEPRTELKVGDPVLVYVLQPE
                     SPEGHAVLSVRRAGLERKWRAMQERFEAGEIVEARVIDHNKGGLIVDLGVRGFVPISQ
                     IVDFPRRPRDEQPRDAAQEIAEKLQPFVGRTLRLKILEVNRKANRLILSEKVAVYEER
                     REKRDELFSSLNVGQRVQGTVRSIAPFGVFVDLGGIDGLVHKSELSWNKVNNPETAYE
                     IGEQVEAEVIDINHERGRISLSIRRLQPDPWQESVAKYNIGDLIDGTVTKLVNFGAFV
                     RIEEGLEGLIHISELSNSRVAHPGDVVQEGQQLKLKIISLDSERHRLGLSLKQTEDRS
                     VPTVQPSYEMTELPGGDGENADRGPRPERAERTPERTDRADRPRSRDRRDRRDRDYRP
                     EDATAEPEEGIDNTMAAAFAESGLLDQFRTDEPEAKPAPTEEATAVDAEAVPAVDAEA
                     APVVDAVAEEPAVEAEAPAAEVAEPAEKAADRKAAKPAAKARKKTAKAAPAEEPAAEE
                     PPAETATNEASASSEPDKG"
     gene            5193..>5365
                     /locus_tag="VGO32_00030"
     CDS             5193..>5365
                     /locus_tag="VGO32_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV7199174.1"
                     /translation="MDRFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVEL"
BASE COUNT          991 a         1761 c         1795 g          818 t
ORIGIN      
        1 gctgtctcaa cgagggatcc ggcgaaattg aaatacctgt gaagatgcag gttacccgcg
       61 gcagggcaaa aagaccccgt ggagctttac tgtagcttga tattggactg aggttcagga
      121 tgtgtaggat aggtgggaga ctttgaagct ggggcgctag cctcggtgga gtcaacgttg
      181 aaataccacc ctttttggat gtcggctcta acggcgaccc gtgatccggg cgccggacag
      241 tgtcaggtag gcagtttgac tggggcggtc gcctcctaaa gagtaacgga ggcgcccaac
      301 ggtaccctca ggctgaatgg aaaccagccg tagagtgcaa aggcataagg gtgcttgact
      361 gcgagaccta caagtcgagc agggacgaaa gtcgggctta gtgatctggt acctccgagt
      421 ggaaggggta tcactcaacg gataaaagct accccgggga taacaggctt atggtgccca
      481 agagttcaca tcgacggcac cgtttggcac ctcgatgtcg gctcgtcgca tcctggggcg
      541 gaagtacgtc ccaagggttt ggctgtccgc caattaaagc tggtacgcga gctgggttca
      601 gaacgtcgtg agacagttcg gtctctatcc gccgtgggcg taggaaactt gcgaggagct
      661 gctcctagta cgagaggacc ggagtggacg aacctatggt gttccagttg tcgtgccaac
      721 ggcattgctg ggtagctatg ttcggcaggg ataagcactg aaagcatcta agtgcgaagc
      781 tcgcctcaag atgaggtttc ccaccgggtt aaccgggtaa gaccgcagcg agactagctg
      841 tttgataggc cgcacgtgta cgcgcagcaa tgcgttcagc gaagcggtac taacggtcga
      901 gggcttgacc tttttcacgc ggccagcacg gagctggacg cggattcact cgaacgacat
      961 caatgcttcc agaatcggtt gacgcgccgt cacaggcgcg ggtcgcaatt gaagattatc
     1021 ccggtgacgc tagcgaagag gctccacccg ttcccatccc gaacacggaa gttaagctct
     1081 tcagcgccga agatactcgg ggggcaactc cctgggaaaa taggtcgtcg ccgggataat
     1141 tccttgtccg gcgccttttt ttcgcgttcg gcagccgccg tcgggcgacc tcccggcccc
     1201 gtatccttga gccgtggacc ccatctgccc cctgctgagc cttgggggtg acccgcgcgt
     1261 ggtctcggcc acgcccgatg acgcgcaccg ctgcctggct gacgggacgc cggcgcccat
     1321 cgagcgcgag taccaggtcc ggttctgcct gaccgcgcgg cacgagacat gtgagcgata
     1381 cctcgctcat ctcgccgacg ttggcccggt cggtcccacc tggcgccctg ccgcccctga
     1441 ttcggcatac ggctcgaccc gcctggtgat ggagtccgcg ccgcgatccg tgatccgccg
     1501 ccgtccgcga cgcttcggga tggccgcgct gttcgtgatc ggactggtcc tcgtcggcgt
     1561 cctggtggcc tggatcggcc tgggatcgat cctcgcccca ccggcgggct cgccgacgcc
     1621 atcggccacc gcttccgcta caccggagga gagcgcgtcg ccgagcccca gcccctcgcc
     1681 gacgcccgag cccacgccaa cgccgatccc gaccccgact ccggtccctg cgccaacgcc
     1741 gatcagctac atcgtccaat cgggtgacac cctgaacgag atcgcctcgc ggttcgggac
     1801 gaccgcccag gccattgcag atttcaatgg gatcaacgtc gccgatccca tccaggtcgg
     1861 ccaggtgctg ctcattcccg tgagctgaac ccaccgactc gcctggaggg ctcagaaggg
     1921 cggttggcag gtccggcaga aggtagttgc ctcgtcgccg ccgatctgac gcagtggccg
     1981 gccacaccgc gggcatggct cgccgccgcg gagatggacc tgcaggaact cgcggtgctg
     2041 ggtctcgatg tggggcggga cgagatcgcg gaggcagacg gtggcgtcgg ccagcaccgc
     2101 gcgcatggcc tcatacaaac gatcgatgtc ctccggggcg aggctccgac gctttcggag
     2161 cggcgccagc cgggccgccc acaggatctc gtcgctgtac gcattcccga tcccggccac
     2221 gaaccgctgg ttgcggagga gcgatttcag ctcgcccggg tgacggcgga tccgggcccg
     2281 aaaggcctcc agcgtgagct ccggatcatc ggcatccggc cccatgtcgg cccagcccgg
     2341 gacgtcgtcc aggcggtcgc gatcgacgag atagagcttg cccagcatct cgcggtcgac
     2401 atagcggagc tcggcgccat cggtaaacgt caggcgcagc ccggtccgtg cccggacctt
     2461 ctgacccggc gcgtcgatca gccagaatcg cccggcgagc atcgggttgg caagcaggag
     2521 ccgctcctcg agccccgcgc gcagcagggg caggatcagg aacttcccac ggcgtccggc
     2581 cgggccgagg gtcatgccgg tcagggactc cagatcggcg gctgtcgccc ggagcaggat
     2641 gggtgtcgga gccgtcgccg cggcgatctc gcgacccgtg gcccgacgct cgagctcctc
     2701 ggccacgatc gtgagatccg ggagttcagg cacgctcgga ctccattgac cctgatggta
     2761 tgctacgccg gcctctgagc aggacaacca gatagcgtgc gggacacccg tcatcgccca
     2821 ggaggagagc accgagtcgt cgccacgagg accaggaccg ttcacaatcc gggttgacgc
     2881 cctccgcatg cggagggttg ttttgttttc agagagaccg ataccgattg gagccatatt
     2941 cacttggacg aagacccgca gcccgcggcc ccgaccgacg agccatcgtc agccggccct
     3001 gatgccgcga tgaccctgtc ggaggagccg acctcctcgc ccacggaggc cgcgaccatg
     3061 gtcgcggatc ccgagcccga tcccaccgcc gaagcactcg atgagctcgc ggtcgaggct
     3121 cgcgccagcg ccgatgtcgc cgaagccgtc gccgcggtcg aggtggcgga ggccatcgag
     3181 gcggtcgcgg tcgcggagga gctggccgag gtcgcggatg cggtcgagtc ggtcgccatg
     3241 gacgaggtca cggccgacga gcccgtggcc gacgagcccg tggccgaggt caccgccgag
     3301 ggagaacccg ccgcggaggt accggtcgag gacgaacggc ccgagccgac gaccatggcc
     3361 gagttcctcg accacccaga ccacgaggtc aagaacctcc gccacggcga cgttgtcgag
     3421 ggagtcgtgg tccggatcga tccggacgag ctgctggtcg atttcggcgg caagtcggag
     3481 ggggtcgtgt ccaaccgcga gctcctcggc cgtcgcggtg gaccgcgcga gggcgacgag
     3541 ccccgcaccg agctgaaggt cggggacccg gtcctcgtct acgtcctcca gcccgagtcg
     3601 cccgagggcc acgccgtcct gtcggtccgt cgtgccggcc tcgagcgcaa gtggcgcgcg
     3661 atgcaggagc gattcgaggc aggcgagatc gtcgaggccc gggtcatcga ccacaacaag
     3721 ggcggtctca tcgtcgacct gggggtccgt ggcttcgttc cgatctccca gatcgttgac
     3781 ttccctcggc gtccgcgcga tgagcagccg cgggacgccg cccaggagat tgccgagaag
     3841 ctccagccgt tcgtgggccg gacccttcgg ctgaagatcc tcgaggtcaa ccgcaaggcg
     3901 aatcggctca tcctgtccga gaaggtcgcg gtctacgagg agcggcgcga gaagcgcgat
     3961 gagctgttca gctcgctcaa cgtgggccag cgcgtccagg gcaccgtccg cagcatcgcg
     4021 ccattcggcg tcttcgtgga tctcggtggt atcgacggcc tcgtccacaa gagcgagctg
     4081 tcatggaaca aggtgaacaa cccggagacg gcctacgaga tcggcgagca ggtcgaggcc
     4141 gaagtgatcg acatcaacca tgagcgcggc cgtatcagcc tgtcgatccg tcgcctgcag
     4201 cccgacccgt ggcaggaatc ggtcgccaag tacaacatcg gcgacctcat cgatggcacc
     4261 gtcaccaagc tcgtgaactt cggcgcgttc gtccggatcg aggaggggct cgagggcctc
     4321 atccacatca gcgagctgtc gaacagccgg gttgcccatc cgggcgacgt ggtccaggag
     4381 ggccagcagc tgaagctgaa gatcatcagc ctcgactccg agcgtcatcg gctgggactc
     4441 agcctgaagc agacggagga ccgatcggtc ccgaccgtcc agccgtcgta cgaaatgacc
     4501 gagctgcccg gcggcgacgg cgagaatgcc gaccgcggtc ctcgcccgga gcgcgccgag
     4561 cgaactccag aacggaccga ccgcgccgat cgcccgcgga gtcgtgaccg ccgcgaccgc
     4621 cgcgaccgcg actaccggcc cgaagacgcg accgccgaac ccgaggaggg aatcgacaac
     4681 accatggccg cggccttcgc cgagtcgggc ctgctggacc agttccgcac cgacgaaccg
     4741 gaagccaagc ccgcacccac ggaggaagcg acagcggtcg atgctgaagc ggtacccgcg
     4801 gtcgacgccg aagcggcacc tgtggtcgac gcggttgctg aggagcccgc ggtcgaggcc
     4861 gaagccccag ccgccgaagt cgcggagccg gccgagaaag ctgccgacag aaaggccgca
     4921 aagcctgcgg ccaaggcacg gaagaagacc gccaaggcgg cgccggccga ggagccggcc
     4981 gccgaagagc ccccggccga gacggcaacc aacgaggcga gcgcatcgag cgagcccgac
     5041 aagggctgag ccggccgcga gccggaatcc gcgccaggcg ccacgcgcca gggtgatcca
     5101 tcgggcctga tggcccgtat cacctattga cccccttcga gcgggatgcg acaccgcacc
     5161 gtagcggtgc cgtacactca aggacgaggc ttatggaccg tttcgacaag ttcaccgacc
     5221 gggcacgcaa ggtcctgaca ctggcccagg atgaagcgca gcgcttcaac cacaactaca
     5281 tcgggaccga gcatctgctg cttggactcg tccgcgaggg cgagggggtc gccgcgcgcg
     5341 tcctcgagaa catgaacgtc gagct
//
LOCUS       DASYGU010000002         9865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_669286, whole genome shotgun sequence.
ACCESSION   DASYGU010000002 DASYGU010000000
VERSION     DASYGU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669286"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGO32_00035"
     CDS             complement(<1..332)
                     /locus_tag="VGO32_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199175.1"
                     /translation="MEGLPDPGQIWNDFTSNPVTQLAATLIVIYVVMLWLGTAYWAFR
                     DMQARSENPILPYFASALIIFFTPLVFLFAWLMYIIIRPRETMAEVYERSLAEESLLA
                     EMEKHRSCP"
     gene            610..2250
                     /locus_tag="VGO32_00040"
     CDS             610..2250
                     /locus_tag="VGO32_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7199176.1"
                     /translation="MLWLLRLLGFLYPVVAAVCLTLLGVMLVRQPPETRGFTVGVVGL
                     AIGLGATAMALTTWFYFALRLSRLAKDLEQTVDKGTPVMLKETGVPAERRLARAFNRA
                     AGTFAVAEAQATRDRLTGIPNRESLLRTLTGEVERANRHSKPLSVAFMDIDRFKSTND
                     TYGHRTGDAVLQQLAGLITRSIRASDIFGRYGGEEFMLILPETTPEDAFTLVEELREM
                     VMRRPLSVASGVLVDATISIGIAGGHSSELQVDRLVDAADAAMYVAKSEGRNRSFLFG
                     SVSDESVVRRAAITPERRDAAAAIGRWASASAAQALASVLAPQPHHRGRPSDMIAALS
                     IGMALHMGLSRVEMDRIRVAALLHDLGKLALPGEILDKPSPLTDVEWQVVGEHPRIGQ
                     VILEQATSMREAVPIVLHHHERYDGTGYPHGLRGEEIPLGARIVAVADAYHAMVHERP
                     YKSALTHAQALAELRRHAASQFDPTVVEVFIAIFGEEVPSDGVEEIEKLHAHAAGGPE
                     SVDPQAAALLQASHWTEPEPAPTGSAKSRARRPAQQAS"
     gene            complement(2264..3031)
                     /locus_tag="VGO32_00045"
     CDS             complement(2264..3031)
                     /locus_tag="VGO32_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV7199177.1"
                     /translation="MTEVQVCRRCGTENQLGVSFCASCGQRLVAADEATMTRPGAPAE
                     SSPCPRCGASNRSGSAFCSECGFNIRPAVASAGGPPAAAPSREGEDTHGGPPAAPAGR
                     AWLGPMVLAIAAAGMATAWLLPFAMGDGSLADQALGVGGYGVAFWTGYPNDAGILQTA
                     YYGVAAPLPILVGLLLVLAVAGILRASPGRIQRLGIVIAIAWCLAFSVLFVVVEIGSG
                     LGEDLINLLRGLSPAGLIGFLAGIIGTIGGTTRLAGG"
     gene            complement(3038..3880)
                     /locus_tag="VGO32_00050"
     CDS             complement(3038..3880)
                     /locus_tag="VGO32_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199178.1"
                     /translation="MATWLANSGRAARLAIDRSDLWFAGAVVAFAGAGWIVLLGTVTP
                     PPDGADAAELGLALAESGWWPWNLVVLAVAIVSGLACLLLVIAFGEVAILIGLADPLT
                     DDPPPTVPRAMAALSLPALLVGLGALAVGWLTTPTIFGLVTGPNADAPLAVRVVAAAW
                     PYLVVLGIGIVLAQALGAARLHRPRRARVGPSLGPLWLVAQAVVTLAAFLVFQLVTAV
                     TLAVLWEPLARRLAGAGLTEPTTLVLLLGFVWIWLVLVIMAGVVQAWISAWWEIELAP
                     RGGG"
     gene            complement(3892..6843)
                     /locus_tag="VGO32_00055"
     CDS             complement(3892..6843)
                     /locus_tag="VGO32_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEV7199179.1"
                     /translation="MSSWFDKLLEEMQRRQMEQDYAREGRPLPPRPPVGPPEEQIPDD
                     DDGGDGPPPPRRLRPRRGRPERTPGNWVRPILFVVGVFILIQVLGGLVQLFTDLAWYG
                     SLGRRDVLVTRFVAELGWFVVGLLLFVVPALASLWVARRLGPQVPIRKVGPVEVPDLS
                     RPAAYVLVGVTVVLGLVSAFAWGGNWQTILLFFNGGNFGTTDASFGRDIGFYVFDLPF
                     WRFVQGWAVLALAAIIGLTVGAYAVAATRWSFHLTAPVRAHVSLLGAGLLLAIAVGYQ
                     LDAAELVYSTRGIGGTVQAAMYTDMNAQVPAYAILTIVAVVSAGLLVANIFFRTLWLI
                     GLAAGAWLVLSIVVGGFYPGAVQRFGVEPNELERERPFLERHLAATREAFDLDSIEER
                     QFTGEQRITRALFEENEATLDNLRLWDYRPLLLTFGQQQILRQYYNFLDVDIDRYQID
                     DAQRQIMLSGREIEIDRLADAARTWTNERLVFTHGYGLTAVRSNAITPEGQPDYLISG
                     INRNPDLPLSEPRIYFGEATTEYVVVRTRTPEFDYPTGDEGGETTSWTGDTGVPVDNV
                     LSRLMFAIRFGDLNLLISDQLTADSAILFRRDIHTRAPELAPFLAYDRDPYLVNADNR
                     LLWIWDAYTTTARYPNAQPLSSFETQFPGANYVRNSVKVVVDAYTGEVHYYVVDPDEP
                     IIAAWSAIFPGLLEPMSDMPESLIPHLRYPEDLFTAQNTTYLLYHLSPTESGAGTFYN
                     QDDQWSIPTQVSDVSGEGGPVAPYYVIMKVPGEDESEFVLIQPLVAANRPNMIAWIAA
                     RMDPGVYGERIEFRFPVDTTTLGPAQVQARINQDSRISEQFTLWSQAGSDVVRGDLLV
                     LPMGDSILYVEPIYLRSTQSSFPEFKRVILADQTRVAFAETLDEGLRQILGEVAIPEP
                     EPGGGGGGGGELPEDVASLIALAGQLYDEAQAALGAGDLGAYQDAVDELGRVLDRIAE
                     LTGTPAPAPSP"
     gene            complement(6871..8547)
                     /gene="guaA"
                     /locus_tag="VGO32_00060"
     CDS             complement(6871..8547)
                     /gene="guaA"
                     /locus_tag="VGO32_00060"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV7199180.1"
                     /translation="METRGRTVHGEPANAGHRVLAPDDLEVETYLEQALKGPIERAAD
                     QPLDPQTIIVLDFGGQYAQLIARRIRELNVYSVLLPHDTPWDEIERRRPVGIILSGGP
                     ASVYDADAPQADPRLWEGRFPVLGICYGVQLMAQQLGGKVAPAERREYGPATIRAAVD
                     EPLLAGLPATAPVWMSHGDSILEPPPGFRGIAASESTPYAAMANDHGLMGLQFHPEVV
                     HTPNGREILANFALHIAGARGDWTAGSFIGSTVATIRAEVGDGKVICALSGGVDSAVA
                     ATLVHRAVGDQLTCIYVDTGLMRKRESELLEATFAQSLGMPLRMVDARERFLARLRGV
                     TDPEEKRRIIGDEFIRVFEEEAASMGPIDFLVQGTLYPDVIESKTVESKAAAKIKTHH
                     NVGGLPADLRFRLIEPLRYLFKDEVRRVGAELGLPEAMIQRQPFPGPGLAIRVIGEVT
                     ASRLATLRDADWIVLDEVKAAGLYGELWQSFAILTPIDTVGVMGDGRTYANVVAVRAV
                     TSDDGMTADWARLPYDVLARISSRIVNEVPGVNRVVYDISSKPPATIEWE"
     gene            8588..9085
                     /locus_tag="VGO32_00065"
     CDS             8588..9085
                     /locus_tag="VGO32_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEV7199181.1"
                     /translation="MADDHEFHAEIRDVGAQQTVCARITAPSDELQDVFRRLPVAVFE
                     RIRATGAEPAGPLFARYHAFGTEQVDVEIGFPVTEPLSDLSPVPEIDAGEIGSSMLPA
                     GRVAYTIHRGPYDGLSDSYDRLHEWIHAAGLSEGSGPWESYVDDPGDMSDMANVRTEI
                     IWPIG"
     gene            complement(9090..9494)
                     /locus_tag="VGO32_00070"
     CDS             complement(9090..9494)
                     /locus_tag="VGO32_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7199182.1"
                     /translation="MRVIRAVDRRFERAGTETFSGEVWMGTNFAADGPGTMNSAEVRF
                     SPGARTHWHSHPDGQVLIVTEGRGRTRVVDGELTAIAAGDAVWISSGVQHWHGADPES
                     SMTHVSITGEGGTVWDGEAVTDAEYDGRGEPG"
     gene            complement(9491..>9865)
                     /locus_tag="VGO32_00075"
     CDS             complement(9491..>9865)
                     /locus_tag="VGO32_00075"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011127590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV7199183.1"
                     /translation="GGMKTGGDIAKAIASGADAVMLGSPFAAALEAPGQGVNWGMAAP
                     SPTLPRGTRITLGERFPLEQILFGPAATTHGTQNLVGALRQSMGALGARTIVDMHRVE
                     MVFAPAIATEGKAHQLASHGDR"
BASE COUNT         1564 a         3408 c         3477 g         1416 t
ORIGIN      
        1 ggacagctgc ggtgcttctc catctcggcc aggagcgact cctcggccag ggaccgctcg
       61 tacacctcgg ccatcgtctc ccggggccgg atgatgatgt acatcagcca tgcgaacagg
      121 aaaacgagcg gcgtgaagaa gatgatcagc gccgacgcga agtacggcag gatcgggttc
      181 tcgctgcgtg cctgcatgtc gcgaaacgcc cagtacgcgg tgcccagcca aagcatcacc
      241 acgtagatga cgatcagcgt tgcggccagc tgggtaaccg ggttgctggt gaaatcgttc
      301 cagatctgac ccggatccgg cagaccttcc attcgcgaaa tgcctcactc ctgaggttcg
      361 tgcggcggca cgaacgccgc gccatcctag cagatgggtc ttgaaatccc tcccgcgcgc
      421 ccatggaccg ccggaagtcc ggggtgggta ctgcaccaga ccgcagcatg gcgcgattgg
      481 ccgtccgacc gatgcatccg cagaagcggg ctgaccatca ggccatggtt cgggcgcgtc
      541 cggtccgcga tgctgtcgcc gacatcatcg acgggaatca ttcccgacat ttgtggactg
      601 gaggaaggct tgctctggct ccttcggctg ctgggcttcc tgtatccggt cgtcgcggcc
      661 gtgtgcctca cgctcctggg cgtgatgctc gtccgccagc cacctgagac gcgcggattc
      721 acggtcggcg tggtcggact ggccattggc ctcggcgcca ccgcgatggc gctgaccacc
      781 tggttctact tcgccctccg cctgtcgcgc ctggccaagg atctcgagca gacggtcgac
      841 aaggggaccc cggtgatgct caaggagacc ggcgtcccgg ccgagcgcag gctggcccgg
      901 gccttcaacc gggcggccgg gacgttcgcg gtggcggagg cgcaggccac tcgcgatcgg
      961 ttgaccggga tcccgaaccg cgagtccctg ctgcgcaccc tcaccggcga ggtggagcgc
     1021 gcgaaccggc actccaagcc gctgtcggtg gcgttcatgg acatcgaccg cttcaagtcg
     1081 acaaacgaca cgtacggtca tcggaccggt gacgccgtcc tccagcagct ggccggcctc
     1141 atcacccgca gcatccgggc cagcgacatc ttcggccgct atggcggcga ggagttcatg
     1201 ctgatcctgc ccgagacgac gccggaggac gcgttcactc ttgtcgagga gctgcgcgag
     1261 atggtcatgc gccggccgct cagcgtggcg tcaggcgtgc tggtggacgc cacgatcagc
     1321 atcggcatcg ccggcggcca cagctcggag ctccaggtcg accgactggt ggatgcggcc
     1381 gatgcggcca tgtacgtggc caagtccgaa ggccgcaacc gcagcttcct gttcggcagc
     1441 gtgtcggacg aatcggtcgt gcgccgggcc gccatcacgc cggagcgccg agacgcggcc
     1501 gccgccatcg gtcgctgggc gagcgccagc gccgcccagg cgctggcatc ggtcctggct
     1561 ccccagccgc accatcgggg ccggccgtcg gacatgatcg cggcgctgtc gatcgggatg
     1621 gcgctgcaca tgggtctgtc ccgggtcgag atggatcgga tccgggtggc cgcgctgctc
     1681 cacgaccttg gcaagctggc gctgccgggc gagatcctgg acaagcccag ccccttgacg
     1741 gatgtcgaat ggcaggtcgt gggcgagcac ccccggatcg ggcaggtcat cctcgagcag
     1801 gcaacgagca tgcgcgaggc ggtgcccatc gtcctgcacc atcacgagcg atacgacggg
     1861 accggctatc cccatgggct gcgcggcgag gagatcccgc tcggggcacg gatcgtggcc
     1921 gtggccgatg cctaccacgc catggtccac gagcgtccat acaagtccgc gctgacccac
     1981 gcccaggccc tggccgagct ccggcgccac gccgcctctc agttcgaccc gaccgtggtc
     2041 gaggtcttca tcgccatctt tggtgaggag gttccctccg acggggtcga ggagatcgag
     2101 aagctccacg cccacgcggc ggggggaccc gagtctgtcg acccgcaggc cgccgccctg
     2161 ctccaggcct cgcactggac cgagcccgag ccggcgccta ccggcagcgc aaagagtcgc
     2221 gcgcgccgtc cggcgcagca ggcctcctag gccagtccgg tcctcagccg ccggcgagac
     2281 gggtcgtccc gccgatggtg ccgatgatcc ccgccaggaa cccgatcaac ccggccggag
     2341 acaggccgcg gagcaggttg atgaggtcct cacccaggcc cgagccgatc tccaccacca
     2401 cgaacaggac cgagaacgcg aggcaccagg cgatggcgat cacgataccc agccgctgga
     2461 tccgccctgg cgacgctcga aggatgcccg ccaccgccag cacgagcagg agtccgacca
     2521 ggataggcag cggagccgcc accccgtagt aggccgtctg caggatcccg gcgtcgttcg
     2581 ggtagccggt ccagaacgcg actccgtatc cgcccacgcc caacgcctgg tccgccagcg
     2641 agccatcgcc catggcgaag ggcaggagcc aggccgtcgc catcccggcg gcggcgatgg
     2701 ccagcaccat cggcccgagc cacgcccgcc cggcgggcgc ggcaggcggc ccgccgtggg
     2761 tgtcctcgcc ctcgcgggac ggcgcggcgg cgggtggacc gccggcggac gcgacggccg
     2821 gtcggatgtt gaacccgcac tcggagcaga acgcggagcc ggaccggttg ctcgcgccgc
     2881 agcgcgggca cgggctggac tcggcgggcg cgccgggccg ggtcatggtc gcctcgtccg
     2941 cggccaccag tcgctggccg cagctcgcgc agaacgagac gccgagctgg ttctccgtcc
     3001 cgcagcggcg acagacctgg acctcggtca ttggtgctca gccacctcct cgcggagcga
     3061 gctcgatctc ccaccaggcc gagatccagg cctggacgac cccggccatg atgaccagca
     3121 cgagccagat ccagacgaag cctagcagca ggacgagggt cgtcggctcg gtcagacccg
     3181 cgccggcgag ccgccgcgcc aagggctccc acagcacggc cagcgtcacg gccgtgacca
     3241 gctgaaagac gaggaacgcg gccagggtga cgacggcctg cgcgaccagc cacagcgggc
     3301 cgagggacgg cccaacccgt gcccggcggg ggcggtggag gcgcgcggcg cccagtgcct
     3361 gggccaggac gatgccgatg ccgagcacca ccaggtacgg ccaggcggcc gcgacgacgc
     3421 ggacggcgag aggcgcgtcg gcgttcgggc cggtcaccag cccgaagatg gtgggcgtgg
     3481 tcagccagcc gacagccagc gcgccgagcc cgaccagcag agcgggcagg ctcagggcgg
     3541 ccatcgccct gggcacggtt ggcggcggat cgtcggtcag cggatcggcc aggccgatca
     3601 ggatcgcgac ctcgccgaac gcgatcacga gcagcaggca cgcgagcccc gagacgatcg
     3661 ccacggccag gaccacgaga ttccatggcc accatccgct ctccgccagc gccagcccga
     3721 gctcggcggc atcggccccg tcgggtggcg gggtgaccgt tcccagaagc acgatccagc
     3781 ccgcgccggc gaaggcgacg accgccccgg cgaaccacag gtcagaccga tcgatggcga
     3841 gccgggcggc gcggccgctg tttgccaacc aggtggccac gtggtccagg tctagggcga
     3901 cggtgccggc gccggcgtcc cggtcagctc cgcgatccgg tccagcacgc gtccgagctc
     3961 gtccaccgca tcctggtacg caccgaggtc gccggctccg agggcggcct gggcctcgtc
     4021 gtagagctgt ccggccaggg cgatcaggga agcgacgtcc tccggcagct ccccgccgcc
     4081 gccaccgccg ccgcccggct cgggctcggg gatcgcgacc tcgcccagga tctggcgcag
     4141 cccctcgtcc agggtctccg cgaatgcgac ccgggtctgg tcggccagga tcacgcgctt
     4201 gaactcgggg aagctggact gggtggagcg gaggtagatg ggctccacgt acaggatcga
     4261 gtcgcccatg ggcaggacca gcaggtcgcc gcggacgacg tccgatccgg cctggctcca
     4321 gagggtgaac tgctcgctga tccgcgagtc ctggttgatc cgggcctgga cctgggccgg
     4381 gcccagggtc gtggtgtcga ccgggaagcg aaactcgatc cgttcgccgt agacgcccgg
     4441 gtccatccgg gccgcgatcc acgcgatcat gttcggccgg ttggcggcca ccaggggctg
     4501 gatcaggacg aactcgctct cgtcctcgcc cgggaccttc atgatcacgt agtacggcgc
     4561 gaccggcccg ccctcacccg acacgtccga gacctgggtc gggatcgacc actggtcgtc
     4621 ctggttgtag aaggtgccgg ctcccgactc ggtgggcgag aggtgataga gcaggtaggt
     4681 cgtgttctgg gcggtgaaca ggtcctcggg gtagcgcagg tgagggatga gcgactcggg
     4741 catgtccgac atcggctcca ggagcccggg gaagatcgcc gaccaggccg cgatgatcgg
     4801 ctcatccgga tcgacgacgt agtagtggac ctcgccggtg taggcgtcca cgaccacttt
     4861 gaccgagttg cggacatagt tcgccccggg gaactgggtc tcgaatgacg acagcggctg
     4921 ggcgttcggg tagcgggccg tggtggtgta ggcatcccag atccagagca gccggttgtc
     4981 ggcgttgacg aggtacggat cgcggtcgta cgcgaggaac ggggccagct ccggcgcccg
     5041 ggtatggatg tcgcggcgga acaggatcgc ggagtccgcg gtcagctggt cgctgatcag
     5101 caggttgagg tcgccaaagc ggatcgcgaa catgaggcgg gacaggacgt tgtcgaccgg
     5161 gacgccagtg tccccggtcc acgaggtcgt ctcgccgccc tcatccccgg tcggatagtc
     5221 gaactccggc gtccgggtcc ggaccacgac gtattcggtc gtcgcctcgc cgaagtagat
     5281 ccgcggctcg ctcagcggca ggtccggatt gcggttgatg ccgctgatca ggtagtcggg
     5341 ctgcccctcg ggcgtgatcg cgttggaccg gaccgcggtc agcccgtagc catgggtgaa
     5401 cacgagtcgc tcgttggtcc acgtccgggc ggcatcggcc aggcggtcga tctcgatctc
     5461 gcgccccgag agcatgatct ggcgctgggc gtcgtcgatc tggtagcggt cgatgtcgac
     5521 gtcgaggaag ttgtagtact ggcgcaggat ctgctgctgg ccgaacgtca ggagcagggg
     5581 gcggtagtcc cacaggcgaa gattgtcgag ggtggcctcg ttctcctcga atagggcgcg
     5641 ggtgatgcgc tgctccccag tgaactgccg ctcctcgatg gagtcgaggt cgaacgcctc
     5701 gcgggtcgcg gccaggtggc gctcgaggaa tgggcgttcg cgctcgagct cgttgggctc
     5761 gacgccgaat cgctggacgg cgcccgggta gaagccgccg accacgatgg acagcaccag
     5821 ccacgcaccg gccgccaggc cgatcagcca cagcgtccgg aagaagatgt tggccaccag
     5881 cagccccgcc gacacgacgg cgacgatggt gaggatcgcg taagcgggga cctgcgcgtt
     5941 catgtcggtg tacatcgcgg cctggaccgt cccgccgatg ccgcgggtgg agtacaccag
     6001 ctcggcggcg tcgagctggt agccgaccgc gatggccagc aggagcccgg cgccgagcag
     6061 gctgacatgg gcacgaaccg gggcagtgag atggaatgac cagcgggtgg ccgctaccgc
     6121 gtatgcgccc acggtcaggc cgatgatcgc ggccagggcg aggacggccc agccctggac
     6181 gaaccgccag aagggcaggt cgaagacgta gaagccgatg tcgcggccga aggacgcgtc
     6241 ggtggtcccg aagttccccc cgttgaagaa caggaggatg gtctgccagt tgccgcccca
     6301 cgcgaaggcg ctgaccaggc cgaggacgac cgtaaccccg accaggacgt acgcggccgg
     6361 gcgcgacaga tcgggcactt cgaccgggcc gacctttcgg atgggaacct gcggaccgag
     6421 gcgacgggcg acccacaggc tggccagggc cgggactacg aagagcagga ggccgactac
     6481 gaaccacccg agctcggcca cgaaccgggt cacgagcacg tcacgccggc caagcgagcc
     6541 ataccaggcg aggtcggtga acagctggac gagaccgccc aggacctgga tcaggatgaa
     6601 gacgccgacc acgaacagga tcggccgcac ccagtttccg ggcgttcgct ccgggcggcc
     6661 gcggcgtggt cggagacggc gcggaggcgg gggcccgtcc ccgccgtcat cgtcgtcggg
     6721 gatctgctcc tcgggcgggc cgaccggggg acgcggcggc agaggacggc cctcgcgggc
     6781 gtagtcctgc tccatctggc gccgctgcat ctcttcgagc agcttgtcga accacgagga
     6841 catcggtccg cgcatcctac ggcggatcgg tcactcccac tcgatggtgg ccgggggctt
     6901 ggagctgatg tcgtatacga cccggttcac gccgggcacc tcgttcacga tccgggagct
     6961 gatccgggcc agcacgtcgt acggcagccg cgcccagtcg gcggtcatgc cgtcgtcgga
     7021 ggtcaccgcc cgaacggcga ccacgttcgc gtaggtgcgg ccgtcgccca tcacgcccac
     7081 cgtgtcgatg ggggtcagga tcgcgaagct ctgccacagc tcaccgtaca gcccggcggc
     7141 cttcacctcg tccagcacga tccagtcggc gtcgcgcagc gtcgcgagcc gcgacgcggt
     7201 gacttcgccg atgacccgga tcgcaagccc tggcccgggg aatggctggc gctggatcat
     7261 ggcctccggc aggcccagct cggcgccgac tcgtcggacc tcgtccttga acaggtagcg
     7321 caggggttcg atcagccgga agcggaggtc ggccgggagc ccgcccacgt tgtggtgggt
     7381 cttgatcttg gccgcggcct tggattcgac ggtcttggac tcgatgacgt cgggatagag
     7441 ggtgccctgg accaggaagt cgatcgggcc catggacgcg gcctcctcct cgaagacgcg
     7501 gatgaactcg tcgccgatga tccgccgctt ctcctcgggg tcggtcacgc cgcgcagccg
     7561 ggcgaggaag cgctcgcggg cgtccaccat ccgcagcggc atgcccaggc tctgcgcgaa
     7621 ggtcgcctcc aggagctcgg actcgcgctt gcgcatgagc ccggtgtcca cgtagatgca
     7681 ggtcagctgg tcgccgaccg cgcggtggac gagggttgcc gccaccgcgc tgtccactcc
     7741 gcccgacagc gcgcagatga ctttcccatc gccgacctcg gcccgaatgg tggcgaccgt
     7801 cgagccgatg aacgagcccg cggtccagtc gccgcgcgca ccggcgatgt ggagcgcaaa
     7861 gttggccagg atctcccgcc cgttcggggt atggaccacc tcggggtgga actggagccc
     7921 catcaggccg tggtcgttgg ccatggccgc gtatggcgtg gactcgctgg ccgcgatgcc
     7981 gcggaacccg ggaggaggct ccaggatgga gtcgccgtgg ctcatccaga ccggggcggt
     8041 cgccggcagg ccggccagca gcggctcgtc aaccgcggca cggatggtgg ccgggccgta
     8101 ctcgcgtcgc tcggcgggcg ccaccttgcc cccgagctgc tgggccatga gctggacgcc
     8161 gtagcagatg cccagcaccg ggaacctccc ttcccacagc cgcggatcgg cctgcggcgc
     8221 atcggcgtca tagaccgatg ccggcccacc ggagaggatg atgcccaccg gccggcgccg
     8281 ctcgatctcg tcccacggcg tgtcgtgggg aagcaggacc gagtacacgt tgagctcgcg
     8341 gattcggcgg gcgatgagct gggcgtactg tccgccgaag tcgaggacga tgatcgtctg
     8401 cggatccagc ggctggtcgg cagcccgctc gatcggtccc ttcagcgcct gctcgaggta
     8461 ggtctcgact tcgagatcgt ccggcgccag gacgcggtgc ccggcatttg ccggctcgcc
     8521 gtggacggtg cgacctcgcg tctccatcgg tcgcagtcta gtcgtggcct gctaggctcg
     8581 atcccgaatg gctgacgacc acgagttcca cgccgagatt cgcgacgtgg gcgcgcagca
     8641 gaccgtctgc gcgcggatca ccgcgccgag cgacgagcta caggacgttt ttcgccggct
     8701 gccggtcgcc gtgttcgagc ggattcgcgc gaccggcgcc gaacccgccg ggccgctgtt
     8761 cgcgcggtat cacgcattcg gcacggagca ggtcgacgtg gagatcgggt tccccgtcac
     8821 ggagccgctg tccgacctgt cgccggtgcc ggagatcgac gcgggcgaga tcggctcgag
     8881 catgctgccc gccggccgcg tggcgtacac gattcatcga ggtccgtacg acggcctatc
     8941 ggacagctat gaccggctcc acgagtggat ccatgctgcg ggcctgtccg agggcagcgg
     9001 gccatgggag tcgtacgttg atgaccccgg cgacatgagc gacatggcca acgttcggac
     9061 ggagatcatc tggcccatcg gctgaatgct cagcccggct cgccacgtcc gtcgtactcc
     9121 gcatccgtca ccgcctcccc gtcccatacc gtcccgccct cgccggtgat cgagacgtgg
     9181 gtcatcgacg attcggggtc ggccccatgc caatgctgga cgccggacga aatccatacc
     9241 gcgtcgccgg ccgcgatcgc ggtcagctcg ccgtcgacca cccgcgtccg accccggccc
     9301 tcggtcacga tcaggacctg cccatcggga tgcgagtgcc agtgcgtgcg cgctcccggc
     9361 gaaaagcgga cctcggccga attcatcgtt cccggaccgt ccgcggcaaa gttcgtcccc
     9421 atccacacct cgccgctgaa cgtctcggtc cccgcccgct cgaagcgtcg gtcgacggct
     9481 cggatgaccc tcaccggtct ccgtggctcg ccagctggtg ggccttgccc tcggtggcga
     9541 tggcgggtgc gaacaccatc tccacccggt gcatgtcgac gatcgtccgt gccccgagcg
     9601 cgcccatcga ctggcgcagg gccccgacca ggttctgcgt cccgtgggtg gtggccgccg
     9661 gaccgaacag gatctgctcc aggggaaagc gctcaccaag cgtgatccgg gtcccgcgcg
     9721 ggagcgtggg agaaggcgcc gccatccccc agttcacgcc ctggccgggc gcctcgaggg
     9781 cggccgcgaa cggcgatccg agcatcaccg cgtccgcgcc ggaggcaatg gccttcgcaa
     9841 tgtcaccgcc ggtcttcatc ccgcc
//
LOCUS       DASYGU010000003         5058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1338669, whole genome shotgun sequence.
ACCESSION   DASYGU010000003 DASYGU010000000
VERSION     DASYGU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338669"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VGO32_00080"
     CDS             complement(<1..496)
                     /locus_tag="VGO32_00080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEV7199184.1"
                     /translation="MTTHRFFVDPGTVSADRFPLPESIAHQVTRVLRLRDGDEIVVLE
                     GDGQQVRCRITGTGLEVLARGLVDAEPRHKLAVAQALLKGDHLEQVVRHGTEVGAFRF
                     ELIVTERCVARDISTGRLARLRAIAREAAEQSERGMVPEVAGPVPLAEVIGRGSVLLF
                     ERSAG"
     gene            complement(493..1398)
                     /gene="prmA"
                     /locus_tag="VGO32_00085"
     CDS             complement(493..1398)
                     /gene="prmA"
                     /locus_tag="VGO32_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV7199185.1"
                     /translation="MRWLALSVEADVEAVEAVSEILGRLGRGSVIEPLRLAADPADEQ
                     ALRPDPAAGYRVTAYVPDDADAAGAVDRTERALWHLQAFDLRPMSALAVTAVDDADWA
                     TAWREGYEPIRIGRITIVPTWLEVPADAELVIRLDPGMAFGTGLHPTTRGCLEELQAL
                     GALPDAVLDVGCGSGILGLAALALGAERVVGYDTDVLAVNATRQNAEVNRRSGQVTAH
                     HETLPDVATETFPLVLANLVAAVLIELAPRLVAHTAPMGTVIASGIIDTRRDEVETAL
                     AAAGLLVADRRVEGEWVTLRLVRSA"
     gene            complement(1422..2546)
                     /gene="dnaJ"
                     /locus_tag="VGO32_00090"
     CDS             complement(1422..2546)
                     /gene="dnaJ"
                     /locus_tag="VGO32_00090"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV7199186.1"
                     /translation="MAVKRDYYEILGVDRGASDEEIKRAFRRAAQKHHPDVDTSTGAE
                     TRFKELNEAYRVLSDRQRRAAYDMFGHAGVDGASAAGEPFGGGFGPFGDIFDAFFGGS
                     PAGGRRRQRVVAGSDLRYELTITLAEAVTGVTREIRFPTLAQCATCAGSGAEPGSEPE
                     RCPECNGTGERRRVSQTILGQMVNITACPRCNGEGRVISTPCHACKGDGRLREDRRIQ
                     VAVPAGIETGQRIALDGQGEAGPRGGPPGDLFVVVAVREEADLVRHGSELYAEVPVTF
                     TQAALGAKVPVRTVDGTEEVEVPPGTQSGQEIRLRGRGMPRLRGAGRGDLHVLVTVVI
                     PAKLTKRERELLQQLGEASGPAVLPKSGPSLGDRLRDLFG"
     gene            complement(2598..3317)
                     /locus_tag="VGO32_00095"
     CDS             complement(2598..3317)
                     /locus_tag="VGO32_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199187.1"
                     /translation="MTTEHLGAPPPIPTSTTTDDAAARAVRVQLLTTEHWSLLATRNL
                     SWNESFARAGMFLTLLTGAVVALALVAQATSFGSGFMTFALLLLPIVFFVGSSTFVRL
                     NEINEEDLVWVKGMNRLRRAYLDIDPGIEPYLITGSTEDLVGVLRTFGAPGGAGIKAP
                     SIRGNVMHAFVTTPVMILIVNCVVAAVLVGLVVGQFGMSMELAAITAVAAFVLTFAAF
                     SAYGARRAAFWMGSEDRAEHG"
     gene            3368..4618
                     /locus_tag="VGO32_00100"
     CDS             3368..4618
                     /locus_tag="VGO32_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7199188.1"
                     /translation="MSDPRQISADHARRFLVRRHLLDPPRSLPPEPASVLRVIDRIGS
                     LQFDPLEVPGARNHDLVLHARIEGYRREWADQWLYGPLGERRLVELYNKMLSIVPMDE
                     LPYYRLSWTRMGSNYAEFLAEHAGLADRIRTHIRDEGPVSTAAFRDVDHRIQWWWDND
                     ETTSTKAARAVMEALFVAGELGISRREGSRRYYDLIDRLVPADLLGRHAPEPEQVRHR
                     LISRHRGVGLMAASAAAEVVWGTGTTPERKLMTASLIEDGTLIPVQVEGLKETRHILA
                     DEWPILKATEQADPGRVPAVTFLAPLGPLMWDRRLVRSLFGFEYIWEVYVPQPKRRHG
                     YYVLPILYGDRFVGRIEPRYERKTGTLHVLGIWFEAGFKRMEEPHFVPALAEALRAYR
                     SFVGASKTIWPRTRVGRELARATR"
     gene            4615..5049
                     /locus_tag="VGO32_00105"
     CDS             4615..5049
                     /locus_tag="VGO32_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7199189.1"
                     /translation="MNTPRWLLRILWLEDRALSKLSGGRMALPNGSGGAVRTLFLRTK
                     GRKTGAKRRNGLYYVDDGSNLIIVASNAGRDQDPAWWINLQAQPDAEVEVGKEVRAVH
                     ARRASPEEAARLYERFIAGLPQYGGYRERTTREIPVVILEPR"
BASE COUNT          770 a         1815 c         1743 g          730 t
ORIGIN      
        1 tgcctgcgga ccgctcgaag agcaggaccg agccccgacc gatgacctcc gccagcggga
       61 ccggtccggc gacctccgga accatgccgc gctccgactg ctcggcggcc tcacgggcaa
      121 tggcgcgaag gcgagcgagg cgcccggtcg agatgtcgcg ggccacgcag cgctcggtga
      181 cgatgagctc gaatcggaat gccccgacct cggtcccgtg ccggaccacc tgctcgaggt
      241 ggtcgccctt cagcagggcc tgcgccactg cgagcttgtg ccgcggctcg gcgtcgacca
      301 gcccacgggc gagcacctcc aacccggtcc ccgtgattcg gcagcggacc tgctggccgt
      361 ctccctcgag gaccacgatc tcgtcgccgt cgcgcaggcg caggacgcgg gtgacctggt
      421 gtgcgatcga ctcggggagc ggaaaccggt ccgcgctgac ggtcccgggg tcgacaaaga
      481 accgatgagt cgtcacgccg agcgcaccag gcggagggtg acccactccc cctcgacacg
      541 gcggtccgcg accagcagcc cggccgccgc gagcgcggtc tcgacctcgt cccgccgggt
      601 atcgatgatc ccggacgcga tgaccgtccc catcggcgcc gtgtgagcca ccagccgcgg
      661 cgcgagctcg atgagcaccg ccgccacgag gttggccagc acgaggggga aggtctcggt
      721 ggcgacgtcc ggcagggtct cgtgatgcgc ggtcacctgt ccgctccgcc gattcacctc
      781 cgcgttctgg cgcgtcgcgt tcacggccag gacatcggtg tcgtagccga ccacccgttc
      841 cgcgcccagt gccagtgccg ccaggcccag gatccccgac ccgcagccca cgtccagtac
      901 ggcgtccggc agggcgccga gcgcctggag ctcctcgagg cagccgcgcg tggtcgggtg
      961 gagtcccgtc ccgaacgcca tcccgggatc gaggcggatg acgagctcgg cgtcggcggg
     1021 aacctcgagc caggtgggca cgatcgtgat ccggccgatg cggatgggct cgtacccctc
     1081 gcgccaggcg gtcgcccagt cggcgtcgtc caccgcggtg accgcgagcg cgctcatcgg
     1141 ccgcaggtcg aacgcctgga ggtgccagag cgcccgctcc gtgcgatcca cagcgccggc
     1201 cgcgtccgcg tcgtcgggga catacgcagt cactcggtag ccggctgcgg ggtccggacg
     1261 aagggcctgc tcatcggcag gatccgcggc aagacggagc ggctcgatca cgctcccccg
     1321 ccccagccgg cccaggatct cgctcaccgc ttcgacggcc tccacatcgg cctcaacgct
     1381 gagggccagc cagcgcatcg gctcgacgct cgtggtcgtc gtcagccgaa gaggtcgcgc
     1441 aggcggtcac ccaggctggg tccgctcttg ggcagcaccg cgggaccgct cgcctcgccc
     1501 agctgctgga ggagctcccg ctcgcgcttg gtgagcttgg ccgggatgac caccgtcacc
     1561 aggacatgga ggtcgcctcg ccccgccccg cggagacgcg gcatccctcg gccgcggagc
     1621 cggatctcct gtccgctctg cgttccaggc ggcacctcga cctcctcggt accgtccacg
     1681 gtccggaccg ggaccttcgc gccgagcgcg gcctgggtga aggtgaccgg gacctcggcg
     1741 tacagctcgg agccatgccg cacgaggtcc gcctcctcgc ggacggcgac gaccacgaac
     1801 aggtcgccgg gaggcccgcc acgcggtccg gcctcgccct ggccatcgag cgcgatccgc
     1861 tgcccggtct cgatccccgc tggcaccgcg acctggatcc gccgatcctc gcgcaagcgg
     1921 ccatcgccct tgcacgcatg gcatggggtc gagatcacgc ggccttcacc gttgcagcga
     1981 ggacaggcgg tgatgttcac catctggccc aggatcgtct gggatacccg acggcgttcc
     2041 ccggtgccgt tgcactccgg acaccgctcc ggctcgctgc cgggctcggc gcccgaaccg
     2101 gcgcaggtcg cgcactgggc cagggtcggg aaccggatct cgcgcgtgac gccggtcacg
     2161 gcctcggcca gggtgatcgt cagctcgtag cgcaggtcgg agccggccac cactcgttgc
     2221 cgtcgccgcc cgccggcggg acttccgcca aagaaggcat cgaagatgtc gccaaacgga
     2281 ccgaagccgc cgccgaacgg ctcccctgcg gcgctcgcgc cgtccactcc ggcgtgaccg
     2341 aacatgtcgt acgccgcgcg ccgctggcgg tcggacagga cgcggtaggc ctcgttcagc
     2401 tccttgaagc gggtctcggc cccggtgctg gtgtccacgt cggggtggtg cttctgggcg
     2461 gcacgacgaa acgcgcgctt gatctcctca tcggaggcgc cccggtcgac accgagaatc
     2521 tcgtagtagt cgcgcttcac cgccatggtc ggcggattat accgattggc actctgaacc
     2581 ccagagtgcc agccggctca cccgtgctcg gcccgatcct ccgagcccat ccagaaggct
     2641 gccctgcggg caccgtacgc cgagaatgcg gcgaaggtca gcacgaaggc cgccacggcc
     2701 gtgatcgcgg cgagctccat ggacatcccg aattgcccga ccaccagccc caccaggacc
     2761 gcggcgacca cgcagttcac gatgaggatc atgaccgggg tcgtcacaaa cgcatgcatg
     2821 acgttcccgc ggatgctcgg cgccttgatg ccggcccctc cgggcgcgcc gaacgtgcgg
     2881 agcacgccga cgaggtcctc ggtcgaccca gtgatgaggt agggctcgat ccccggatcg
     2941 atgtccaggt atgcgcgccg cagccggttc atgcccttga cccagacgag gtcctcctcg
     3001 ttgatctcat tcagccggac gaaggtcgag ctgcccacga agaagacgat aggcaagagc
     3061 agcagggcga aggtcatgaa cccactgccg aaggaggtgg cctgcgcgac gagagccagc
     3121 gccacgactg cgccggtgag cagggtcagg aacatgccag cccgggcgaa cgactcgttc
     3181 caggacagat tccgggtggc cagcaggctc cagtgctcgg tggtcaggag ctgaacgcgg
     3241 acggctcgag cggccgcgtc gtcggtggtg gtcgaagttg ggatgggagg tggcgctccg
     3301 aggtgctcgg tggtcattgg tggagtgtgc cgcgctcgcg cccggttcgt ctagcatcgg
     3361 tcagcccgtg tctgatccgc gccagatctc agccgaccac gcacggcggt tcctcgtccg
     3421 tcgccacttg ctcgacccac cgcgctccct gccgccggag ccggcgtcgg tcctgcgcgt
     3481 catcgaccgt atcggtagcc tccagttcga cccgctcgag gtgcccggcg cccgcaacca
     3541 cgacctcgtg ctccacgcac ggatcgaggg ctaccgccgg gagtgggccg atcagtggct
     3601 ctatgggccg cttggggagc ggcggctggt ggagctgtac aacaagatgc tcagcatcgt
     3661 cccgatggac gagctcccct actaccggct gtcctggacc cgcatgggct ccaactacgc
     3721 cgagtttctc gcggagcacg ccggcctggc cgatcggatc cggacccata tccgggatga
     3781 gggcccggtc tcgaccgccg ccttccgcga cgtggaccat cggatccagt ggtggtggga
     3841 caacgacgag acgacctcga ccaaggccgc ccgagcggtg atggaggcgc tgttcgtggc
     3901 cggcgagctg ggcatctccc ggcgtgaagg cagccgccgc tactacgacc tcatcgaccg
     3961 cctggttccg gccgacctgc tgggccggca cgctccagag ccggagcagg tgcgccaccg
     4021 cctcatctcg cggcaccggg gggtgggcct catggccgcg agcgcggccg cggaggtcgt
     4081 gtggggcacc gggacgaccc cggagcggaa gctcatgacc gcgtccctca tcgaggacgg
     4141 caccctcatc ccggtccagg tagagggcct gaaggagacc cgccatatcc tcgccgacga
     4201 gtggccgatc ctgaaggcca ctgaacaggc ggaccccggc cgggtaccag cggtgacctt
     4261 cctggcgccg cttggcccgc tcatgtggga ccggcgcctg gtgcggagcc tcttcgggtt
     4321 cgagtacatc tgggaggtct acgtccccca gcccaagcgg cgccacggct actacgtgct
     4381 gccgatcctg tacggcgacc ggttcgtcgg ccgaatcgag ccacgctatg agcgaaagac
     4441 cggcacgctc catgtcctgg gcatctggtt cgaggcgggc ttcaaacgga tggaggagcc
     4501 gcacttcgtg ccggccctgg ccgaggcgct ccgcgcctat cggtccttcg tcggtgcgag
     4561 caagactatc tggccgcgca cgcgggttgg tcgcgagctg gcgagggcca cgcggtgaac
     4621 acgccccggt ggctgctgcg tatcctctgg ctcgaggatc gggccctctc gaagctcagt
     4681 ggcgggcgga tggccctccc caacggcagc ggaggcgcgg tgcggaccct gttcctgcga
     4741 acgaaaggcc gcaagaccgg cgcgaaacgg cggaacgggc tgtactacgt cgacgatggc
     4801 tcgaacctga tcatcgtggc ctcgaacgcg ggtcgcgacc aggatccagc ctggtggatc
     4861 aatctgcagg cccagcccga tgccgaggtc gaggtgggga aagaggtccg cgcggtccac
     4921 gcccgccggg catccccgga ggaagccgcg cggttgtacg agcgcttcat tgccgggctc
     4981 ccccagtacg ggggctaccg ggagcgaacc acgcgcgaga tcccggtcgt catcctcgag
     5041 ccgcgctgag gcgacccg
//
LOCUS       DASYGU010000004         9320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_772883, whole genome shotgun sequence.
ACCESSION   DASYGU010000004 DASYGU010000000
VERSION     DASYGU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772883"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="clpX"
                     /locus_tag="VGO32_00110"
     CDS             complement(<1..1017)
                     /gene="clpX"
                     /locus_tag="VGO32_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV7199190.1"
                     /translation="MTTKTKAPYRCSFCGKSQEQVRKLIAGQGVYICDECINLCQEII
                     DEEMAEAPRASRTPPAPLPSPRKIKEALDQYVISQDAAKKVLSVAVYNHYKRVNAGSA
                     VDEVELGKSNVLLVGPTGSGKTLLAQTLARVLDVPFCIADATSLTEAGYVGEDVENIL
                     LRLIQAADFDVQRAQRGIVYVDEIDKIARKTDNPSITRDVSGEGVQQALLKILEGTVA
                     NVPPQGGRKHPHQDFIQIDTTNILFICGGAFEGLERIVEERVGRRGIGFGSEAQRNPE
                     ERRLLLDQLMPDDLLRYGLIPEFVGRMPVVVSLAALSRDDLVKVLTEPKNAVVRQFAK
                     FLSLD"
     gene            complement(1193..1750)
                     /locus_tag="VGO32_00115"
                     /pseudo
     CDS             complement(1193..1750)
                     /locus_tag="VGO32_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            complement(1756..3165)
                     /gene="tig"
                     /locus_tag="VGO32_00120"
     CDS             complement(1756..3165)
                     /gene="tig"
                     /locus_tag="VGO32_00120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV7199191.1"
                     /translation="MTTVETRPEPGSRVRLEIEVPADEVSRHFVTAYRHLAARTRVPG
                     FRPGKAPPNVIERYVGRAEVIAEAVEHIVGEGYDAAIDQTDLIPIDQPQIEIDTETVR
                     EGDPVKFSATVAVRPTVTLGAYTGYSFGLEVPPVTDEDVETVVEELRGQQATLRPVDN
                     RGAANGDLVAVSFRGTIDGEPFDGGTADRLPLILGEDRMIPGWEDQVVGLAVEETREF
                     DLPFPDDYRVEDLAGKTAHFSVTLTDLRERVLPELDDAFAASVSDGQNVAELRAEIRD
                     ALVRRADAEARHQFGDRIIDFATANATVELPEVMIANEVEIMRDELRARLAEQRIGID
                     QYLELSRQSPEELMAELREPASRRVKTLLVLSAIAEQEDIDASDAEIDAEVDRQLAAY
                     PDEPRLREYLASRRGRSYLRMTLRNRTLVDSLINRAAPAAQADSEAEPAAEAASTADP
                     SPEPEPETKTDSKSESSAE"
     gene            complement(3198..3281)
                     /locus_tag="VGO32_00125"
     tRNA            complement(3198..3281)
                     /locus_tag="VGO32_00125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3246..3248),aa:Leu,seq:tag)
     gene            complement(3314..4033)
                     /locus_tag="VGO32_00130"
     CDS             complement(3314..4033)
                     /locus_tag="VGO32_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199192.1"
                     /translation="MGATLDETRVELEAQRARVRGTATRLEAATRESLNIRAIVGRHP
                     ARTIGLAAGAAFFFLGGPSKTVHAVRRAFGAAADGEKAYAALPASLRSFVDASATGFG
                     ESKAEAKGQMALALLAWRADPKNRKKADRLVSETLTPPGPERAFWALVEVAAVTAAGI
                     IAKQVVARSMAGGAVRALLGRPGPIVPPMPPPATEAAIDAKAPSAKPKTATGPSGYTG
                     WSGRSEVAGPARAPAPTEGAR"
     gene            complement(4003..4419)
                     /locus_tag="VGO32_00135"
     CDS             complement(4003..4419)
                     /locus_tag="VGO32_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV7199193.1"
                     /translation="MTTNPTDSPPGAGPRSLLGQLFEQVTELGRLYVSAGRQEVEAGI
                     NDIKIALIFFGVTVMFLAVAVVLVILLIVSTIAAITGLPLWATALIVLFVVLVLAALF
                     GWLGYRRVVDVRLMPEKTIEAAKEDLEWAQHWTKRG"
     gene            4498..5139
                     /locus_tag="VGO32_00140"
     CDS             4498..5139
                     /locus_tag="VGO32_00140"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEV7199194.1"
                     /translation="MSRRRSAAPQRRSRASEEALQRRAGSSSSRLGGSSLAILGIGAL
                     VIVGITAIALFALLAPGASATVGTPQTMDGREHIEEGTAGGPYSSVPAASGPHWPQPL
                     EWGVYPSAIRQEPAIHNLEHGGIVIWYQADQVSPADVTGLAEYTRSWNGSERYKVILA
                     PWAGEDFGYPMAILAWTWLLYLDEVDTDLMDQFIDQHYGDAPEPGGGPGPPAL"
     gene            5176..5250
                     /locus_tag="VGO32_00145"
     tRNA            5176..5250
                     /locus_tag="VGO32_00145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5208..5210,aa:Lys,seq:ctt)
     gene            5331..6629
                     /locus_tag="VGO32_00150"
     CDS             5331..6629
                     /locus_tag="VGO32_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019061035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV7199195.1"
                     /translation="MPQVPIERERLDNGLRVILSEDRRTPIVGVNLWYDVGSRNEERG
                     RTGFAHLFEHMMFQGSANVAKGEHFALIQAAGGTLNASTWTDRTNYFETLPSHELELA
                     LWLESDRMASLLPAMTQEKLDNQRDVVKNERRLSVDNQPYGTAEERMQALMWPEGHPY
                     HHSTIGSMEDLSAASMDDVSRFFATYYAPNNAVLTVAGDFDRDAAMARIERHFGPIPA
                     NPTLPAPPSGDVPDSLGGEVREVVADQVELPRIHLAYRIPPFGTDAHEAFNILSDVLG
                     TGRASRLYRSLVRERQLAADVAAFVFPWAHGSTALGIWATARPEVSVETLEAQLLAEI
                     DQLSTDGPTDEELARVRTLHAAAVEAGLEQIGERADRISQYACLFDKPDMVNTEVARY
                     DALTAADIRAALGDRISADNRLVLTYVPDEDQTEDGEAAA"
     gene            6626..8014
                     /locus_tag="VGO32_00155"
     CDS             6626..8014
                     /locus_tag="VGO32_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV7199196.1"
                     /translation="MSTPNALRPPATEPRPYHFPDFSHERLPNGLTVWTVPLSDRALV
                     SVHLIVDGGAAAESEAEAGIASLAAETLVTGTRRLDGHAFAEATERLGIEMSADSSWD
                     LARAGFLAVAGSFEAGLELLAEMIHEPRFDEGEFDRLRQERLADILQARSEPGRLADE
                     YFLRQVYPDDVPYGRLGAGSPESVEPLTVAQARAHHQRHWRPDRAHLVVAGPVSATEA
                     RQTVASAFGHWEGESPGHRSIRAADRGGRRIVIVDRPGSVQSELRIGHVGIARGDADY
                     FSAIVMASLLGGTFSSRLNQRLREDLGYTYGARAAFDPRRSAGPFSARAAVHTEVTAD
                     AVRETLAILDGLQAAPPSADELRGVTDFLVGIFPLRFETTAGAAAGIEPVAVYDLDRG
                     WWATYRDRIEAVGPAEVHAAAQAHIRPADSLILLAGDAAAVRPSLEAANLGPVEVIRP
                     ADGMTDAEGAEG"
     gene            complement(8011..9222)
                     /locus_tag="VGO32_00160"
     CDS             complement(8011..9222)
                     /locus_tag="VGO32_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV7199197.1"
                     /translation="MPSSPDVIHQDLDFYAVLDADPSATFDELRVAFRRAVLRHHPDR
                     SGADIPLATRRTSLLNRAWSELRDPIRRRAYDNALERDEAPTLAWPLAAGEPGRTTPV
                     RARRARRPRVAAEPSPWHQPAWRSVAGFRVPAAVFLDQPEAQRRWIVEHYIDGQDWRD
                     HRERYWLRFAAAYYQERGRLEDWVGIAERLVEVDPSFDTLARSGLREGYQLTDRHLAG
                     AAFLAEAGERWPPEAPARAWVQRELRVLLGDFREKHVRRGPSADRAANATLLLDYLAG
                     FAMEPTVADLRAAYLAQRRAGNRGQADILFERLLAQPVTDGDGWYTRVQLLTDAGELE
                     RASSLLAAVARGDHPDALDPGRVRGEPFVRLARARARLQRAQVKSRRNGVGADGTGRN
                     GTGRTGRASRD"
BASE COUNT         1348 a         3232 c         3261 g         1479 t
ORIGIN      
        1 gtcgagcgac aggaacttcg cgaactggcg tacgaccgcg ttcttgggct cggtcaggac
       61 cttgaccagg tcgtcgcgcg acagggcggc caggctgacc acgaccggca tccgacccac
      121 gaactcggga atgagcccgt atcgaagcag gtcgtcgggc atgagctggt ccaggagcag
      181 acggcgctcc tcgggatttc gctgcgcctc ggagccaaag ccgatccccc gccgtccgac
      241 gcgctcctcg acgatccgct cgagcccctc gaatgcgccg ccgcagatga acaggatgtt
      301 ggtggtgtcg atctggatga agtcctggtg cgggtgcttc cgtccaccct ggggtgggac
      361 gttggcaacc gtcccctcca agatcttgag caacgcctgc tggacgccct cgccggagac
      421 gtcgcgggtg atggacgggt tgtccgtctt gcgtgcgatc ttgtcgatct cgtcgacgta
      481 cacgatcccc cgctgcgcgc gctggacgtc gaagtcggcg gcctggatga gccggagcag
      541 gatgttctcg acgtcctccc caacgtagcc ggcttcggtc aggctggtgg cgtcggcgat
      601 gcagaacggg acgtccagga cgcgggccag ggtctgggcc agcagggtct tgcccgagcc
      661 ggtgggcccg accagcagga cgttgctctt gcccagctcg acctcgtcaa ccgccgaccc
      721 ggcgttgacc cgcttgtagt ggttgtagac cgcgaccgac aggaccttct tggctgcgtc
      781 ctggctgatg acgtactggt ccagcgcttc cttgatcttc ctcgggctgg gcagcggggc
      841 gggcggggtc cggctggcgc ggggcgcctc cgccatctcc tcgtcgatga tctcctggca
      901 gaggttgatg cactcgtcgc agatgtacac gccctgcccg gcgatcagct tgcggacctg
      961 ctcctggctc ttcccgcaga acgagcagcg gtatggagcc ttggtcttgg tcgtcatcgc
     1021 cggccgagga tagcacgggc ctgttcagat tcccgatgga ccaatcggcc catcccggcg
     1081 cctacttctt ggcgcgggcg tccgcggcgg tggcttcctt ggcggtcgga accgtgcccc
     1141 cggcggcctg cttcatgctg atcagcgact ccccggtgcc cgcgaacacg gcatcgatga
     1201 tgccgtacgt cttggcttcc tccgagctca tgaagtagtc gcgttcggcg tccttcttca
     1261 ccttctcgta ctcctggccg gtgtggtcgc cgaggatgcg catgagccgt tcggtcaggg
     1321 tcagcatctc cttgacctgg atctcgacgt cgggcgtgtt gccccggaat ccgccggatc
     1381 cctggtggag catgatccgg gagttcggca gcgcgaaccg cttgcccttg gcgccggcgg
     1441 ccaggaggac tgcccccatg ctcgccgcct ggccgataca gatggtgctg accggagcct
     1501 tgaggtactg catcgtgtcg tagatcgcca ggccgctggt cacgatcccg ccgggcgagt
     1561 tgatgtacag catgatgtcc ttgtcctggt cctcgctctc gaggaacagg agctgggcga
     1621 tgatcaggtt ggcgacgtgg tcttcgatcg gctcgcccag gaagacgatc cgctccttga
     1681 gcagccgcga gtagatgtcg aacgcgcgct ctccgcgggc gctgctctcg atgaccatgg
     1741 ggacgagcat ctggttcact ccgcgctgga ttcggatttg gaatcggttt tggtttcggg
     1801 ttcgggctcc ggtgacggat cggccgtcga cgccgcctcg gccgccggtt cggcctcgga
     1861 gtccgcttgg gcggcgggcg ccgcccggtt gatgaggctg tcgacgagtg tacggttccg
     1921 gagcgtcatg cgcaggtacg accggccgcg gcgcgacgcg aggtactcac ggaggcgcgg
     1981 ctcgtccggg taggcggcga gctggcggtc cacctccgcg tcgatctccg cgtcggacgc
     2041 gtcgatgtcc tcctgctcgg caatggccga caggacgagg agggtcttga cccggcggct
     2101 cgcgggctcg cgaagctcag ccatcagctc ctcgggactt tgacgcgaca gctcgagata
     2161 ctggtcgatc ccgatccgct gctcggccag acgggcccgg agctcgtcgc gcatgatctc
     2221 gacctcgttc gcgatcatca cctccggcag ctcgaccgtc gcattcgcgg tcgcgaaatc
     2281 gatgatccgg tcgccgaact ggtggcgcgc ctcggcatcg gcccggcgca cgagggcatc
     2341 gcgaatctcg gcccggagct cggcgacgtt ctggccatcg ctcaccgatg cggcgaacgc
     2401 gtcgtcgagc tcgggcagga cccgttcgcg tagatcggtg agcgtgacgc tgaagtgggc
     2461 ggtcttgccc gccaggtcct cgacccgata gtcgtcgggg aagggcaggt cgaactcgcg
     2521 ggtttcctcc acggcgaggc cgaccacctg atcctcccag ccggggatca tccggtcctc
     2581 gcccaggatg agcggaagcc ggtccgcggt gccgccatcg aatggctcgc cgtcgatcgt
     2641 gccccggaac gacacggcca ccaggtcacc gttcgccgcg ccgcgattgt ccaccgggcg
     2701 gagggtcgcc tgctggccgc ggagctcctc gacgaccgtt tccacgtcct cgtcggtgac
     2761 cggcgggacc tcgagcccga acgagtagcc ggtgtaggcg cccagggtca cggtcgggcg
     2821 gacggcgacc gtcgccgaga acttcaccgg atcgccttcc cggacggtct cggtgtcgat
     2881 ctcgatctgc ggctggtcga tcgggatcag atcggtctgg tcgatggccg cgtcgtaacc
     2941 ctcgcccacg atgtgctcga ccgcctcggc gatcacctcg gctcggccga cgtagcgctc
     3001 gatcacgttg ggcggcgcct tcccgggtcg gaagccggga acccgtgtcc gggccgcgag
     3061 atgccgatag gcggtcacga aatggcggct cacctcgtcg gcgggcactt cgatctcgag
     3121 ccgcacccgg gagccgggct cgggccgcgt ttcgacggtg gtcatggagt cgcggtcacg
     3181 ggcagagcct cagttgatgg tgggcgaggc gagattcgaa ctcgcacgtc ttgcgacacc
     3241 agctcctaaa gctggcgcgt ctgccgttcc gccactcgcc ccggttgaga tgctcggcgg
     3301 ccggccgagt ctatcagcgg gctccctcgg ttggcgccgg cgcgcgggcc ggaccggcga
     3361 cctccgatcg tcccgaccac ccggtgtaac cggacggccc agtcgcggtc ttgggcttgg
     3421 cgctgggcgc cttggcgtcg atggccgcct ctgtcgccgg cggcggcatg ggagggacga
     3481 tcggccccgg ccggcccagg agcgctctga ccgcgccccc ggccatgctc cgagcaacga
     3541 cctgcttggc gatgatgccg gcggcggtca ctgccgcaac ctcgaccagg gcccagaacg
     3601 cgcgctcggg ccctggtggg gtcagcgtct cgctgaccag gcgatccgcc ttcttgcgat
     3661 tcttgggatc ggctcgccag gccaggagcg caagcgccat ttggcccttc gcctcggcct
     3721 tcgactcccc gaacccggtc gcggacgcgt ccacgaagga gcggagggat gccggcaatg
     3781 cggcgtaggc cttctcgccg tcggcggcgg cgccgaacgc ccgcctgacg gcgtggacgg
     3841 tcttgctcgg ccccccgagg aagaagaagg cggcgccagc ggcgaggccg atggtccgcg
     3901 ccgggtgacg gccgacgatg gcccggatgt tcagcgactc gcgggtggcc gcttcgaggc
     3961 gagtcgcggt cccgcgcacg cgggcccgct gcgcctcgag ctctacccgc gtttcgtcca
     4021 gtgttgcgcc cactcaagat cctccttggc agcctcgatc gtcttttccg gcatgagacg
     4081 cacgtcgacc acccgacggt agccaagcca tccgaacagc gccgccagca ccagcacgac
     4141 gaacaggacg atgagagcgg tggcccacag cggcaggccc gtgatcgcgg cgatcgtgct
     4201 cacgatgagc aggatgacga gcacgacggc cacggcaaga aacatgaccg tcacgccgaa
     4261 gaagatcagc gcgatcttga tgtcattgat cccggcctcg acctcctggc ggccggcgga
     4321 cacgtacagc cggcccagct cggtcacctg ctcgaagagc tgacctagga ggctgcgcgg
     4381 gcccgcgccc gggggcgagt cggtcgggtt agtcgtcacg gactgccgat tctagcaatg
     4441 cggtcctagg accccgtccc ggaccattct cccgacctcc gctattctct ccggccagtg
     4501 tcccgtcgaa gaagcgccgc gccccagcgc cgctcccgcg ccagtgagga agccctccag
     4561 cgccgggcgg gctcgtcgtc ctcgcggctc ggtggctcgt cgctcgcaat cctcggcatc
     4621 ggggccttgg tcatcgtcgg gatcacggcc atcgccctgt tcgcccttct agcgcccggg
     4681 gccagcgcga cggtcgggac cccgcagacc atggacggcc gggagcacat cgaggagggc
     4741 accgccggcg ggccgtactc ctcggttccg gccgcgtccg gaccccattg gccgcagccg
     4801 ctggagtggg gcgtctatcc gagcgcgatc cgccaggagc ccgcgatcca caatcttgag
     4861 cacggcggaa tcgtgatctg gtaccaggcc gaccaggtga gtccggcgga cgtcaccggg
     4921 ctggcggagt acacgcggtc gtggaatggc tccgagcggt acaaggtcat cctcgcgcca
     4981 tgggctggtg aggatttcgg ctatccgatg gccatcctgg cctggacgtg gctcctctac
     5041 ctcgacgagg tcgacaccga cctcatggac cagttcatcg accagcatta cggggatgcg
     5101 ccagagccgg gcggaggccc cggtcctccc gccctgtagt tgcgtgcggc gtcaccggtc
     5161 gggcatcatc ggcgggggcg cctagctcaa cggcagagca agtgactctt aatcactagg
     5221 ttcagggttc gaatccctgg gcgctcacca gcactcatcg acgtcgtacc ggacgggccc
     5281 ggatcgggcc cgtcgctgtg cctgaatacc ggtcacgtga ggccttcgcc atgccccagg
     5341 ttcccatcga gcgggagcgg ctcgacaacg gtctacgggt gatcctgtcc gaggaccgac
     5401 ggacgccgat cgtcggcgtg aacctgtggt acgacgtcgg aagccgcaac gaggagcgcg
     5461 gccggaccgg gttcgcccat ctcttcgagc acatgatgtt ccaggggtcg gccaacgtcg
     5521 ccaagggcga gcactttgcc ctgatccagg ccgcgggcgg aacgctgaac gcctcgacct
     5581 ggaccgatcg gacgaactac ttcgaaaccc tcccatccca tgagctcgag ctggccctgt
     5641 ggctggagtc ggaccggatg gccagcctcc tgccggcgat gacccaggag aagctggaca
     5701 accagcgcga cgtcgtgaag aacgaacggc ggctgtcagt cgacaaccag ccgtacggca
     5761 ccgccgagga gcgcatgcag gcgctgatgt ggccggaggg tcacccgtac caccactcta
     5821 cgatcgggtc aatggaggat ctgagcgcgg catcgatgga cgacgtgagt cgcttcttcg
     5881 cgacgtacta cgcaccgaac aacgccgtcc tgaccgtcgc cggcgacttc gatcgggacg
     5941 cggcgatggc gaggatcgag cgtcatttcg ggccgatccc ggccaacccc accctgccgg
     6001 ctccgccgag cggtgacgtt ccggactcgc tgggcggcga ggtccgcgag gtcgtggccg
     6061 accaggtcga gctgccgcgc atccacctcg cctaccgcat tccgccgttc gggaccgacg
     6121 cccacgaagc gttcaacatc ctgagcgacg tcctgggcac gggccgtgcc tcgcggctct
     6181 accgctcgct ggtccgagaa cgacagctcg cggccgatgt ggccgccttc gtcttcccgt
     6241 gggcccatgg ctccaccgcg ctcggcatct gggccaccgc acgccccgag gtatcggtcg
     6301 agacgctcga ggcgcagctg ctggccgaga tcgaccagct gagcaccgat ggtccgaccg
     6361 acgaggagct ggcccgggtc cgcaccctgc atgccgccgc cgtcgaggcg gggctcgagc
     6421 agatcggtga gcgggcggat cggatcagcc agtacgcgtg tctgttcgac aagccggaca
     6481 tggtcaacac cgaggtcgcc cgctacgacg cgctgacggc cgccgacatc cgggcggcgc
     6541 tcggcgaccg gatcagtgcc gacaaccggc tggtgctcac ctacgtgccg gacgaggacc
     6601 agaccgaaga cggcgaggcc gcggcatgag cacgccgaat gcgcttcgcc cgcccgccac
     6661 cgagccgcgt ccgtaccact tcccggactt cagccacgag cggctgccaa acggattgac
     6721 ggtgtggacg gtcccgctct ccgaccgtgc gctggtgagc gtccatctca tcgtcgacgg
     6781 aggcgcggca gccgaatccg aggcggaggc cgggatcgca tcgctggccg cggagacgct
     6841 cgtcaccggc acgcgccggc tcgatggcca tgccttcgcc gaggccaccg aacgactcgg
     6901 gattgagatg tcggccgact catcgtggga cctcgctcgt gccggcttcc tggcggtggc
     6961 cggcagcttc gaggccgggc tcgagctcct ggccgagatg attcacgagc cacgcttcga
     7021 cgagggggag ttcgaccggc ttcgccagga gcggctggcg gacatcctcc aggcccggtc
     7081 cgagccgggc cgcctggctg acgagtactt cctccgtcag gtgtacccgg acgacgtgcc
     7141 ctatggccgc ctcggcgccg ggtcgcccga gtcggtcgag ccgctcacgg tggcccaggc
     7201 tcgggctcac caccagcggc actggcggcc ggatcgcgca cacctggtgg tggccgggcc
     7261 ggtcagcgcc acggaggcgc gtcagaccgt cgcatcggcc ttcggtcact gggaaggtga
     7321 gagtcccggc catcgctcga tccgggcggc cgaccgtggc ggccgacgaa tcgtcatcgt
     7381 cgaccgaccg ggatcggtcc agagcgagct gcgcatcggc catgtcggga tcgcgcgagg
     7441 cgatgcggac tacttctcgg ccatcgtcat ggcgtcgttg ctgggcggga ccttcagcag
     7501 ccgcctgaac cagcgcctcc gcgaggatct gggctacacg tacggcgccc gtgcggcgtt
     7561 cgacccgcgg cgctcggccg gaccattcag tgcccgagcg gccgtccaca ccgaggtcac
     7621 ggccgatgcc gtgcgcgaga cgctcgccat cctggacggg ctgcaggctg cgccaccatc
     7681 ggccgacgag cttcgcgggg tcaccgactt cctggtcggg atcttcccac tccggttcga
     7741 gacgaccgcc ggagcggccg ccggaatcga gccggtggcc gtctacgacc tggatcgcgg
     7801 gtggtgggcg acctaccgcg accgcatcga ggccgtcggc ccggcggagg tgcacgccgc
     7861 cgcccaggcc cacatccggc cggcggattc gctgatcctg ctcgcgggcg acgcggcggc
     7921 ggttcggccg tcgctggagg ccgcgaacct cggcccggtg gaagtcatcc ggccggcgga
     7981 cggcatgacg gacgcggagg gcgccgaagg ctagtcccgg ctcgccctcc cggtgcgccc
     8041 ggttccgttc cttcccgtcc cgtcggcgcc cactccgttt cggcgcgact tcacctgcgc
     8101 ccgctggagg cgtgcccgtg cccgagcgag gcggacgaac ggttcgcccc ggacgcgccc
     8161 cggatcgaga gcatcggggt ggtcgccccg cgccacggcg gcgaggaggg aggacgctcg
     8221 ttcgagctcc ccggcatcgg tgaggagctg gacccgggtg taccacccgt ccccgtcggt
     8281 caccggctgc gcgagcaagc gctcgaacag gatgtccgcc tggccgcggt tcccggcccg
     8341 ccgctgggcg aggtacgcgg ctcgcaggtc ggcaacggtc ggttccatcg caaacccggc
     8401 caggtagtcg aggaggaggg tcgcgttggc cgcccggtcc gcggatggac cgcggcggac
     8461 gtgcttctcg cgaaagtcgc ccagcaggac ccgaagctcg cgctggaccc aggcccgagc
     8521 tggcgcttcg ggcggccacc gctcgccggc ctcggccagg aacgccgcgc cggcgaggtg
     8581 ccggtcggtg agctggtacc cctcccgcag gcccgatcgg gcgagcgtgt cgaacgaggg
     8641 gtcgacctcg accaggcgct ccgcgatgcc gacccagtcc tcgagccgtc cgcgctcctg
     8701 gtagtaggcg gccgcgaagc ggagccagta ccgctcgcgg tggtcgcgcc aatcctggcc
     8761 gtcgatgtag tgctccacga tccaccggcg ctgggcctcc ggctggtcga ggaatacggc
     8821 ggccggcacg cggaagccgg cgacggagcg ccacgcgggc tgatgccacg ggctgggctc
     8881 ggccgccacg cgcgggcgcc gtgcccgccg cgcgcggacc ggggtcgtcc gaccgggctc
     8941 gcccgcggcc aggggccagg cgagggtggg tgcctcgtca cgctcgaggg cgttgtcgta
     9001 tgcccgtcgc cggattggat cgcgcagctc ggaccacgcc cggttcagca gcgaggtgcg
     9061 ccgcgtggcc agcgggatgt cggcgccact gcgatcggga tggtggcgca gcacggctcg
     9121 ccggaatgcc acgcgcagct catcgaacgt ggcggagggg tccgcgtcga gaacggcgta
     9181 gaaatcgaga tcctggtgga tgacgtccgg cgacgagggc atccgacgag tgtacgggtc
     9241 cgtgggatgg gtcgggattg ttagactcct cggcaatggc tgcgaccacc cgccctgcgc
     9301 ggccacgcgc cgggcacctc
//
LOCUS       DASYGU010000005         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1339183, whole genome shotgun sequence.
ACCESSION   DASYGU010000005 DASYGU010000000
VERSION     DASYGU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339183"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VGO32_00165"
     CDS             <1..590
                     /locus_tag="VGO32_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199198.1"
                     /translation="WRVDAATGVATAVADGPFGPEVTVLADGDVLAIRTSSVEAPTWA
                     AAERIDLDGRPAVPVAGTKDDSGLVYRATLLVDGSVALVRHRVGGGVDVVRVAPDGLE
                     MPLADSPHPGVTISPAGDRLAWAIDGTVWLGSVGDESAPLAVGRGTSGSFSPDGSLLM
                     VIGSDAVSVVDLAGARRSDLAGSACWLGGGRGCRP"
     gene            587..1576
                     /locus_tag="VGO32_00170"
     CDS             587..1576
                     /locus_tag="VGO32_00170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV7199199.1"
                     /translation="MSGIYRRPAVMAILVASLLAVPPRAAATDDFTLPFVNPAVPLNY
                     GVDRDPRVGYQLDWTGQLWHDNAAHFGRVYDQHTGIDYGLASLSLVASARNGTVVDIE
                     EGFGTNDHGAAGNFVRVRHPDGRDTLYYHLAQNGALVSVGQAVLAGQTIARSGCSGNC
                     SGPHLHFELLQLSGGVWRSVDPQGGHLWTTWPGRVPYLAAYTAESNSSTVAIKRLATS
                     SHWVEFRNTGGRSWSRDASGSRLLLGTWNPAARSSVFRAADWPSYWIATYLDQATVAP
                     DGIGRFTFGLRAPSTTGSYSEAFNLRVDPVSWFDHARLGGYYVPLYVYSGQQP"
     gene            complement(1583..2065)
                     /locus_tag="VGO32_00175"
     CDS             complement(1583..2065)
                     /locus_tag="VGO32_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEV7199200.1"
                     /translation="MAGADERFAAAAAAAGLDVTIHRFPEGTRTAEDAARAVGCAVGQ
                     IVKSLVFLADGSPVVALVSGADRLDTGRLATALNATTVHRADGDAVRDATGYAIGGVP
                     PFGHARPLPVLMDRGLLVHEVVWAAAGRPDAVFAVTPAALERASGALVTDLAELPSQP
                     "
     regulatory      2106..2212
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2204..2875
                     /locus_tag="VGO32_00180"
     CDS             2204..2875
                     /locus_tag="VGO32_00180"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV7199201.1"
                     /translation="MKAVIVADGAHAPLDRHALAGADLVIAADGGAEWLARIGAVPDR
                     LVGDLDSIDHDRVTTLTDRGVTIERHPVDKDASDLELSVAAAVAAGADDIVVLGALGG
                     DLDHLAGNLLLLGSELVAGRRLRLVHDRTTARMLIGPGELELDATTGSRVSIFAVGDA
                     ADGVTTRGLQWPLADARLAGGASLGLANVVRDPPAGVAVRAGRLLVVQIEPDESTPES
                     MEGET"
     gene            2872..3441
                     /locus_tag="VGO32_00185"
     CDS             2872..3441
                     /locus_tag="VGO32_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV7199202.1"
                     /translation="MRWRTVDIVVTAALGVAFGVVFWAWGLLFSAVAAPAFAPPLYIL
                     SGVWLIPAVLAPLIVRLPGAALLAETMAAAVSALLGSVWGLDTLMSGLMQGAGAELVF
                     ALALYRRWTLPITLLAAAGAGVGEWIHDMTFYFVGVPIEIQLAYGAWMLVSAVLVAGL
                     GSWYLVKLLRPTGVLAAFPAGQDVARSTG"
     gene            3455..>3886
                     /locus_tag="VGO32_00190"
     CDS             3455..>3886
                     /locus_tag="VGO32_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199203.1"
                     /translation="MTRPVVAASGWGWRHPGRSSWAVRHLDLAIGEGERVLLLGASGS
                     GKSTLLTHIAGLVDDRGGGEAEGSLGVLGGSPQAARARTAFLMQDPEAQLVMSRAGDD
                     VAFGLENRGVPTNDIWPRVRAALDTVGWRYGPNRPTDQLSGG"
BASE COUNT          510 a         1389 c         1386 g          601 t
ORIGIN      
        1 tgtggcgcgt ggacgcggcc actggcgtcg caaccgcggt cgcggatgga ccgttcggcc
       61 cggaggtcac cgtcctggcc gatggggacg tgctggccat ccgaacgtcg tcggtcgaag
      121 cgccgacctg ggccgcagcc gagcggatcg accttgatgg ccgacccgcg gtgccggttg
      181 cggggacgaa agacgattcc gggctcgtgt atcgcgcgac cctgctggtc gacgggtcag
      241 tcgcactcgt ccgccatcgg gtcgggggcg gcgtcgacgt cgttcgcgtt gctccagacg
      301 ggctcgagat gccgctggcc gacagcccgc atccgggagt cacgatcagc cccgccgggg
      361 accgtctggc atgggcaatc gatggcactg tctggctggg atcggtcggc gacgagtccg
      421 cgccgctggc cgtcggccgt ggcacgtcgg gcagcttctc tcccgatggg tccctgctca
      481 tggtcatcgg atccgacgcg gtcagcgtcg tcgacctcgc cggcgcgcga cgctcggacc
      541 tggccggatc ggcctgctgg ctgggcggtg ggcgggggtg tcggccatga gcggcatcta
      601 ccggcggccc gccgtgatgg ccatcctggt tgccagtctg ctcgcggttc cgccgcgggc
      661 tgccgccacg gacgacttca ccctgccgtt cgtcaacccg gccgtcccgc tgaattacgg
      721 cgtggaccgc gatccgcggg tcgggtacca gctggactgg accgggcagc tatggcatga
      781 caacgccgcc cactttggtc gggtctacga tcagcacact gggatcgact acgggttggc
      841 gtcgctcagc ctcgtcgcgt ccgcccgaaa cgggacggtg gtcgacatcg aggagggctt
      901 cggtacgaac gaccatggcg ctgccgggaa cttcgtgcgc gttcgccacc ccgacggccg
      961 cgacaccctg tactaccacc tcgcccagaa cggagcgctc gtgtcggttg ggcaggcggt
     1021 gttggccggg cagacgattg cccgctccgg gtgcagtggc aattgcagcg gcccgcatct
     1081 tcacttcgag ctgctccagc tgagcggcgg tgtctggcgg tcggtcgacc cgcagggcgg
     1141 ccacctgtgg accacatggc cgggccgagt cccatacctc gccgcctaca cggccgagtc
     1201 gaacagcagc acggtggcca tcaagcgtct cgccacgtcc agccattggg tcgagttccg
     1261 caacaccgga ggccgcagct ggtcgcggga cgcgtccggc agccggctcc tgctcggcac
     1321 ctggaatccc gccgcgcgct caagcgtgtt tcgggcagcc gactggccgt cgtactggat
     1381 cgcgacctac ctcgaccagg cgaccgtggc acctgacggg atcggccgat tcacctttgg
     1441 gctccgtgcg ccatcgacca cggggtcgta ctcggaggcc ttcaaccttc gcgtggatcc
     1501 ggtgtcctgg ttcgaccacg ccaggctggg cggctactac gtccccctgt acgtgtactc
     1561 cggccagcag ccgtgatccg cctcagggct gggagggaag ctcggccaga tcggtcacga
     1621 gtgcgccgct cgcgcgctcg agggcggccg gggtcaccgc gaacacggcg tccggccgac
     1681 cggccgcggc ccagaccacc tcgtggacca ggagcccgcg gtccatgagg accgggagtg
     1741 gccgggcgtg gccaaagggc ggaacaccac cgatcgcgta gccggtggca tcgcgaaccg
     1801 cgtcaccgtc cgctcggtgg accgtggtcg cgttcagcgc ggtggccagc cgcccggtgt
     1861 caagccgatc cgcgcccgaa acgagggcca cgaccgggct cccgtccgcc aggaagacga
     1921 gcgacttcac gatctggccg accgcgcagc caacggcgcg tgcggcgtcc tcggcggtcc
     1981 gcgtcccctc cgggaatcgg tggatggtca cgtccagccc cgccgctgcc gcggcggcgg
     2041 cgaatcgttc gtcagcgccg gccatgggtc cgatggtacg cgcagtgata cgctgcggcc
     2101 gacagggata tcgacagggg agcgcctcgg cgctgagagt gcggccaccc ggccgcagac
     2161 cctcgaacct gacctgggtc atgccagcgt agggagtcgc accgtgaagg cagtcatcgt
     2221 cgcggacggg gcgcatgcgc ccctcgatcg ccacgccctg gcgggtgccg acctggtcat
     2281 cgccgccgac ggcggagcgg agtggctggc acgcatcggc gccgtcccgg atcggctcgt
     2341 cggcgacctg gactcgatcg accacgaccg cgtcaccacc ctcaccgacc gcggcgtcac
     2401 catcgagcgt catccggtcg acaaggacgc ttccgacctc gagctgagcg tcgccgccgc
     2461 ggtcgccgcg ggcgcggacg acatcgtcgt gctcggcgcg ctgggcgggg acctcgacca
     2521 cctcgcgggc aatttgctct tgctcggctc ggagctcgtc gccggtcggc gactgcgcct
     2581 cgtccacgac cggaccacgg cccggatgct gatcggaccg ggcgagctcg agctggacgc
     2641 gaccaccgga tcgcgggtca gcatctttgc ggtcggcgac gccgccgacg gtgtcaccac
     2701 ccgcggcctc caatggccgc tcgccgatgc ccggctggcg ggcggcgctt ccctggggct
     2761 ggcgaacgtc gtccgcgacc caccggccgg cgtcgcggtc agagccggcc gcctgctcgt
     2821 cgtccagatc gagccggacg aatccacccc ggaatccatg gagggcgaga catgagatgg
     2881 cgaacggtcg acatcgtggt cacggccgcg ctgggcgtgg ccttcggtgt cgtcttctgg
     2941 gcctgggggc tgctgttcag cgcggtcgcg gcgccggcgt ttgcgccccc gctctacatc
     3001 ctgtccgggg tgtggctcat tcccgccgtc ctggcgccgc tcatcgtccg gctgccgggc
     3061 gccgcgctgc tggccgagac gatggccgcc gcggtgtcgg ccctgcttgg atcggtctgg
     3121 ggcctggaca cgctgatgtc ggggctgatg cagggcgctg gagccgagct cgtcttcgcc
     3181 ctggccctct atcggcgctg gacgctgccg atcaccctcc tggccgcggc cggcgccggc
     3241 gtcggcgagt ggatccacga catgaccttc tacttcgtcg gggtcccaat cgagatccag
     3301 ctcgcctacg gggcctggat gctggtcagc gcggtcctcg tcgccggcct gggctcctgg
     3361 tacctcgtga agctcctgcg cccgaccggt gtcctggccg ccttccccgc cgggcaggac
     3421 gtggcgaggt caaccggctg agccaggaag ggtcatgacc cgccccgtcg ttgcggccag
     3481 cggatggggt tggcgccatc cgggccggag cagctgggcg gtccgccatc tcgaccttgc
     3541 cattggcgaa ggggagcgtg tcctgctcct cggagcctcg ggttccggca agtcgaccct
     3601 tctcacccac atcgcgggcc tggtcgacga ccgtggggga ggggaggcgg aaggcagcct
     3661 gggcgtgctc ggcggaagcc cgcaggccgc ccgagcccga accgcgttcc tcatgcagga
     3721 ccccgaggcc cagctcgtga tgtcacgcgc cggggatgac gtcgcgttcg gcctcgagaa
     3781 ccgcggcgtc ccgaccaacg acatttggcc ccgtgtccgg gccgccctgg acaccgtcgg
     3841 ctggcgctac ggcccgaacc gaccgaccga ccagctctcc ggcggc
//
LOCUS       DASYGU010000006         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1082118, whole genome shotgun sequence.
ACCESSION   DASYGU010000006 DASYGU010000000
VERSION     DASYGU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082118"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VGO32_00195"
     CDS             complement(<1..166)
                     /locus_tag="VGO32_00195"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEV7199204.1"
                     /translation="MVKLDRVIRAVDAHAGGEPGRVIIGGVSDVPGATMLEKRNHLAE
                     HGDRLRHLMLR"
     gene            220..1266
                     /locus_tag="VGO32_00200"
     CDS             220..1266
                     /locus_tag="VGO32_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV7199205.1"
                     /translation="MRLGLIPENLIDRVGLATGFPPPAWTEAYAPLYARAIVAATELG
                     VFDAIGAGSLSAAEVATACATDPRATERLLNLLVSMRYLRFRDGRYRLAKSTARWMVS
                     DASDGIRDLVLMKRLEWRWMEGLENYLRSGTPVDVHATMSSEDWDAYQRGMRAQANIA
                     GPRLARLIPVPRGARDMLDIGGSHGYWSVLLCRRHPGLHSTVFDLPAAVEWAAPILAR
                     EGMGDRVVLKAGDALTDDLGREAYDLIIMFSLVHHFDDATNRLLVARAGEALRPGGRM
                     IIFEPLRPPPGRGVQAGPFFDLYFGMTSESGTWTLDEMTAWQAAAGLRPRSQPFALRL
                     IGEAGLAIADKPPA"
     gene            complement(1263..2873)
                     /locus_tag="VGO32_00205"
     CDS             complement(1263..2873)
                     /locus_tag="VGO32_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV7199206.1"
                     /translation="MPRNVRIASIGTALLLAACGSESPSPSPSASSAQSAAPSAPAPT
                     PDLSAYEGLWADATAETIGTTAEYTNRVELGDINGDGLPDILFANGGDYDGPSPDGPV
                     ASRVFINRGPGQPFIETPQVMAGITGYTRVIKVRDLNNDGYNDILLGNTWQTQSQLLL
                     NNGEGYTNATAQLPQVNLNVGDLDVGDVDADGDLDLVLVDWAAGSPFESGGGPVHLWL
                     NDGAAQFTEATAAQMPTTLIGFSWDLELVDVDNDWDLDLAVSAKVSDTSYLFENDGAG
                     SFTDVTGDALPHFANNYEFEPMDLNGDGYLDLVTINDGDNVTGNGGQEHVFANNGDGT
                     FSDMTESWWPDAANPSEDDNAEIFLDVDSDGDADFLLFSLTGVDRLLVNDGTGRLSLV
                     TTAIDAAASRGSLGLAVADLDGDGRLDIVESQGEVVGSFDERVYLATEAMAPDTAAPH
                     IWTDLATDASGTVTVHARVTDGLSPYVAGHPATVWLTWDGGDDAAGVLSWYGEYLFRI
                     TADVPAGATGLAICAADQSGNEACTEAN"
     gene            2948..3688
                     /locus_tag="VGO32_00210"
     CDS             2948..3688
                     /locus_tag="VGO32_00210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7199207.1"
                     /translation="MSWKDLFSGHAEDYARFRPSYPPALFAWLAEVSPGRGLAVDLGT
                     GNGQAAVSLADHFARVIGIEPSAEQLAHAATHPRVEYHVATAEATGLTDAAADLLLAA
                     QAFHWFERDGFFAEARRVVRPGGVLALVSYALSRITPEVDAVVDELYRSLDPYWEPER
                     RTVEDGYASVDVPFPALTAPAFAMESTWTVDDLVGYLGTWSALRKALAVGGRNPLDDV
                     GPQLAAAWGEEPRREVLWPLAVRAFRVD"
     gene            complement(3699..4064)
                     /locus_tag="VGO32_00215"
     CDS             complement(3699..4064)
                     /locus_tag="VGO32_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199208.1"
                     /translation="MSHSVEHLATLMDQLVDLLRRQGEEHWADWVEADARAVRARDGW
                     GLEHFLAAFGGGGSLWDLVFHPINGNAATDAEGRAATEQLHELLEEAHPIALDMSREL
                     NEAEEAAGPALEYSGEASN"
BASE COUNT          617 a         1431 c         1476 g          720 t
ORIGIN      
        1 cgcggagcat cagatggcga aggcggtcgc cgtgctcggc caggtggttc cgtttctcga
       61 gcatcgtcgc ccccggcacg tcggacacgc caccgatgat gacccggccc ggctctccgc
      121 cggcgtgcgc gtccacggcg cggatgaccc ggtccagctt caccacggca tggtggcaca
      181 ggcggtggcc cggacccggc gcggcgtacc atcggcccca tgcgacttgg gctgatcccc
      241 gagaacctga tcgaccgtgt tggccttgcc accggattcc cgccgccggc ctggaccgag
      301 gcctatgcgc cgctctatgc ccgggccatc gttgcggcca cggagctcgg agtgttcgac
      361 gcgatcggcg cgggtagcct gtccgcggcc gaggtggcca ccgcctgcgc gaccgatccg
      421 cgcgccacgg agcgactgct caacctgctg gtgagcatgc ggtacctgcg gttccgcgat
      481 gggaggtacc ggctcgccaa gagcaccgcc cgctggatgg tgagcgacgc gtccgacggg
      541 atccgcgacc tggtcctcat gaagcggctg gagtggcgtt ggatggaagg cctggagaac
      601 tacctgcgat ccggtacccc ggtcgacgtc cacgccacga tgtcgtccga ggactgggat
      661 gcctaccagc gggggatgcg agcccaggcc aacatcgcgg gacctcgcct cgcgcggctg
      721 ataccggtgc ccaggggtgc gcgcgacatg ctcgacattg gcggatcgca cggctactgg
      781 tcggtgttgc tgtgtcgccg ccaccccggg ctgcattcga ccgtatttga tctccccgcc
      841 gccgtcgagt gggcagcgcc cattctcgcg cgtgaaggga tgggcgaccg cgtggtgctg
      901 aaggccgggg atgccctgac cgatgacctc ggccgggagg cttacgacct gatcatcatg
      961 ttcagcctgg tgcatcactt tgacgacgcc acgaatcgcc ttctggtcgc cagggccggc
     1021 gaagcgcttc gtcccggcgg ccggatgatc atcttcgagc cgctccggcc cccgccaggt
     1081 cgcggcgtac aggcggggcc attcttcgac ctctacttcg gcatgaccag cgagtcagga
     1141 acgtggacac tggacgagat gaccgcctgg caggccgctg ccggactgcg gcctcgcagc
     1201 cagccattcg ccctccggct catcggggag gccggactgg cgatcgccga caagcctccg
     1261 gcctagttgg cctcggtgca ggcctcgttg cctgactgat cggccgcgca gatcgcgagt
     1321 ccggtggcgc cggccggaac gtcggccgtg atccggaaca ggtactcgcc gtaccacgac
     1381 aacacgccgg ccgcgtcgtc gccgccatcc cacgtgagcc acaccgtggc cgggtgtccc
     1441 gcgacgtatg gcgatagtcc gtcggtgacc cgggcgtgga ccgtgaccgt gccgctagcg
     1501 tcggtggcca gatcggtcca gatgtgtggg gcggctgtgt cgggggccat cgcctcggtc
     1561 gcgaggtaca cccgctcgtc gaaggagccg accacctcgc cctgcgactc gacgatgtcg
     1621 agccttccgt cgccgtcgag atcggcaact gccaggccca acgagccgcg gctggccgcc
     1681 gcgtcgatcg cggtggtgac cagcgacagc cggcccgtcc cgtcattgac gagcagccga
     1741 tcaacgccgg tcagtgagaa caggaggaaa tcggcgtcac catcggagtc gacgtccagg
     1801 aagatctccg cgttgtcgtc ctcgctcggg ttggcggcat ccggccacca tgattcggtc
     1861 atgtcgctga acgtgccgtc gccgttgttg gcgaacacgt gctcctggcc gccattgccg
     1921 gtgacgttgt ccccgtcgtt gatcgtgacc aggtccaggt acccgtcgcc gttgaggtcc
     1981 atgggctcga actcgtagtt gtttgcgaag tgggggaggg cgtcgccggt gacatcggtg
     2041 aagctcccgg ccccgtcgtt ctcgaacagg tagctggtgt cggagacctt ggccgagacg
     2101 gcaaggtcca gatcccagtc gttgtccacg tcaaccagct cgaggtccca cgagaagccg
     2161 atcagggtgg tcggcatctg ggcggccgtg gcctcggtga actgcgcggc gccgtcgttc
     2221 agccacaggt ggaccgggcc gccaccgctt tcgaacggcg atcccgccgc ccagtcgacg
     2281 aggacgagat ccaggtcgcc atcggcgtcc acgtcgccga cgtccagatc gccgacgttc
     2341 agattcacct gcggcagctg ggcggtggca ttcgtgtagc cctcaccgtt gttcaggagc
     2401 agctggctct gggtctgcca ggtgttgccc agcaagatgt cgttgtaccc gtcgttgttc
     2461 aggtcgcgga ctttgatgac gcgcgtgtat ccggtgatcc cggccatgac ctggggtgtc
     2521 tcgatgaacg gctggccggg accccggttg atgaacaccc ggctggcgac cggcccgtcc
     2581 ggcgagggcc cgtcgtagtc accgccgttg gcgaacagga tgtccggcag tccgtctcca
     2641 ttgatgtcgc ccagctccac ccggttggtg tactccgcgg tggtgccgat cgtttcggcc
     2701 gtcgcgtcgg cccacagtcc ctcgtatgcg ctcaggtcag gcgttggggc gggagccgat
     2761 ggtgcggcgg actgcgccga cgacgccgat ggcgatgggc tcgggctctc cgaaccgcac
     2821 gccgcgagca gcaacgcggt gccgattgag gcgatgcgga cgttccgtgg catgaggcga
     2881 ccattttcgg atggccacgc ccacgcgggc aatgtgtttt cacaccatcg ttgtctacag
     2941 tgagccgatg agctggaagg acctgttctc cggccacgcg gaggactacg cccgattccg
     3001 cccgtcctac ccgccggcgc tgtttgcgtg gctggcggag gtcagccccg gacgcggcct
     3061 ggcggtcgat ctgggcacgg gcaacgggca ggccgcggtg agcctggccg accacttcgc
     3121 gcgggtgatc ggcatcgagc ccagcgccga gcagttggcc catgccgcga cccatccgcg
     3181 ggtcgagtac cacgtggcca ccgcggaggc gacgggactg accgatgccg ccgccgacct
     3241 gctcctcgcg gcgcaggcat ttcactggtt cgaacgcgat ggcttcttcg ccgaggcgcg
     3301 acgggtcgtc cgccccggag gcgtgctcgc cctggtcagc tacgccctgt cgcggatcac
     3361 gcccgaggtg gacgccgtgg tggacgagct gtaccggagc ctcgacccgt actgggagcc
     3421 ggagcggcgg acggtcgagg atgggtacgc atcggtcgac gtgccgttcc cggcgctcac
     3481 agcacctgcc ttcgcgatgg agagcacgtg gacggtggac gatctcgtcg gctacctggg
     3541 gacgtggtcg gccctgcgca aggccctggc ggtcggaggt cggaatccgc tcgacgatgt
     3601 tggccctcag ctggcggcgg cgtggggtga ggagccgcga cgggaggtcc tctggccgct
     3661 cgcggtccgg gcattccgag tcgactgacc cggaacgatc agttcgaggc ttcccccgag
     3721 tactccagcg ccgggcccgc cgcctcttcc gcctcgttca gctcccgcga catgtccagc
     3781 gcgatcgggt gagcctcctc gaggagctcg tgcagctgct cggtggcggc ccgaccctcg
     3841 gcgtcggtcg ccgcattgcc gttgatcggg tggaagacga gatcccagag gctcccgcca
     3901 ccaccgaacg cggccaggaa gtgctcgagg ccccagccgt cgcgcgcccg gacggcgcgg
     3961 gcatcggcct ccacccagtc agcccagtgc tcctcgccct ggcggcgaag gaggtcgacc
     4021 agctggtcca tgagcgtggc gaggtgttca acgctgtgag acatggaccc ccatatcttg
     4081 gctcatgttg actcgagcgc gagcagcaga agcaggttga tcaccgtcgc cgctgccggg
     4141 acggccacga aatagaggag atgaccgatt gcggtgcggc tcacgattgg gtctttccgg
     4201 tcgcctgcgc agcttcgagt cgggcgatcg catcggtcag ctct
//
LOCUS       DASYGU010000007        11749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1545264, whole genome shotgun sequence.
ACCESSION   DASYGU010000007 DASYGU010000000
VERSION     DASYGU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545264"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..531
                     /locus_tag="VGO32_00220"
     CDS             190..531
                     /locus_tag="VGO32_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008714895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7199209.1"
                     /translation="MQIVPPDFDRMFGALGDATRRDIVRRAIDGEEGVAELATHYPMS
                     FAAVQKHVAILERAGLVTKERFGRRKVVRANLDGLLMARRLLDRYEELWLGRIDRMTE
                     LVTENPKETNR"
     gene            528..1034
                     /locus_tag="VGO32_00225"
     CDS             528..1034
                     /locus_tag="VGO32_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7199210.1"
                     /translation="MTVTSVRKDLEALTLTLDAEFDASAQRVWQLWSDPRQLERWWGP
                     PTYPATFTKHDLAPGSHVEYHMTGPSGDQPRGYWDIVEVAPPHRLVFLDGFANEDGTP
                     NADMPVNTALVTIQEIGNGRTRMSIESRYPSTAAMEQNLAMGMEQGLTEAVGQIDAIL
                     AESAVPQA"
     gene            1058..1942
                     /locus_tag="VGO32_00230"
     CDS             1058..1942
                     /locus_tag="VGO32_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7199211.1"
                     /translation="MTATTIEPTTRTLPALGAVITYDIRPNAETTEPILFLIGSPMGA
                     GGFGTLAGNFTDRTVVTYDPRGSERSVKDDPTTESTPDQHGDDLHRVIEALGAGPVDL
                     FASSGGAVNALALVAAHPENVRLLVAHEPPAAQVLPDREAALATTRAIHETYQKGGWG
                     AGMAHFIAVVGHKGPFPDGYADQPGPDPATFGMPTEDSGDRTDVMLGQNIITCTHYQP
                     DFDALRAASTRIVIAAGIGSEGEMANRAAHAVAERLGTELVDFPSDHGGFLGGEYGQT
                     GDPDAFAAKLREVLATEV"
     gene            complement(1953..3311)
                     /locus_tag="VGO32_00235"
     CDS             complement(1953..3311)
                     /locus_tag="VGO32_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV7199212.1"
                     /translation="MGRLFLYAAGVTSVILLSLIFAILAAAEQAPGSLASPAQSGEIV
                     GEITISAFDLGFEPSTVDVAEPGRYTVHFMNDGAVLHDITFEGATAQSAEPGEMIMFD
                     VTIPAGGTTFLCSVPGHASGGMTGIVTVAGVGPPTGGDDHGGPAPDSDVEPDPDAPPY
                     VLYPAEAPPRLEGTVHDIELVIEEKPMTVAQGDDGGFVQAVWTFNGTVPGPVIRVRVG
                     DTIRVHLVNTETSAVAHSIDFHASQVAWNDEMTSINPGEEKVYEWTADYAGVWMYHCG
                     TSPALHHIANGMYGMVIVEPAEGLPPVDAEIAVVQSEWYLGPQGEPASLTKAAAAAPA
                     PDYVVWNGVANQYLDNPIAVGTGDRVRVFVLNAGPSIDSSFHVVGTIFSSVIKEGVHL
                     TPGNPGNFGSQAVDLSPAQGAIIEMVMPEDGLYPMVTHAFNFVGRGALGLFQAGDGDP
                     LN"
     gene            complement(3331..3876)
                     /locus_tag="VGO32_00240"
     CDS             complement(3331..3876)
                     /locus_tag="VGO32_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199213.1"
                     /translation="MVKTGGSTRLQVSLRTGLEVDDIGRLLDAGGPDAWLGDAISAGP
                     AGRRRYATDLKAGHRIGTRLTVHKSAYVDIGSLDRTDQTLRAEVSWRSASLAPLFPVF
                     SGWLTARPGEIAIDGRYVPPGGALGSVADRALMHRAARYTADWLLGELARAASGSDGG
                     PGLPLRPGQVLNSHRNPSQSP"
     gene            4030..4539
                     /locus_tag="VGO32_00245"
     CDS             4030..4539
                     /locus_tag="VGO32_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEV7199214.1"
                     /translation="MTRILVTTASKHGSTREIGDAIAAVLESMGIEAIAKDVDQVATV
                     EDVDGVIVGSGVYAGHWLASATAFVDRFQVELQQRPVWLFSSGPLGDPARPAEDPAEV
                     PTAMASSGALGHRLLAGRLEKTDLGFAERAIVNVVRAPYGDFRDWPEIRGWALEIAGT
                     FTRVRANAG"
     gene            4804..5178
                     /locus_tag="VGO32_00250"
     CDS             4804..5178
                     /locus_tag="VGO32_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7199215.1"
                     /translation="METSRSRVAVIDEARSNDAFRRVLITGIYEQVVVMAIPPGGEIG
                     DEVHPETDQVLVFVEGEAEARLDGEITRVGGNDLVYVRAGTRHNFVNTGPSPLRLYTI
                     YAPPHHPPGTVHQTKADADAAD"
     gene            5233..6396
                     /locus_tag="VGO32_00255"
     CDS             5233..6396
                     /locus_tag="VGO32_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEV7199216.1"
                     /translation="MHGAAEAVDKSLRERDALDAAVQGIAGVLDLDRVLQLIVDRVRE
                     LADGEYAALGIIGPDESLERFITSGIDAAERNRIGALPTGRGLLGVIIRESQSLRLAD
                     LATDERRSGFPAHHPPMHSFLGVPVRIRDRAIGNFYLTNKRGAAEFSLDDQRLVERFA
                     LPAAIAIDTARLHGEVQRLAIMADRDRIGRDLHDGVIQRLYAVTLSLDDVPELMDEAP
                     GEARERVERSIQALQSAIGDIRAFIYDLRPVEVSATDLHGALVELAEEAEMNGATEVQ
                     VQIDGDAPIPSAQAIEMLAIAREALSNVGRHAAARHATIHLAISGDAARLTITDDGRG
                     FDVEAPRDGGHRGLGNMRDRAGALGGRLTVTSVAGEGTRVVAEIPGQSSDPVA"
     gene            6393..7076
                     /locus_tag="VGO32_00260"
     CDS             6393..7076
                     /locus_tag="VGO32_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7199217.1"
                     /translation="MTNPETAGPSAAPLRLLLVDDHEVVRQGLRALIERRPAFQVVAE
                     AGTVADAIAAARHFKPDLVLMDVRLPDGSGVEACRAIREDNPAIRVVMLTSYPDEEAV
                     LAAILAGASGYLLKEIRGRDLVAALESVGRGDSLLDPAVTERVLERVRRMAAGDAQDE
                     LAELTAQERKILMLVAEGKTNREVAAEVFLSDKTVKNYVSSILSKLGLQRRAQAAAFV
                     AKHRLGDGT"
     gene            7184..7762
                     /locus_tag="VGO32_00265"
     CDS             7184..7762
                     /locus_tag="VGO32_00265"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV7199218.1"
                     /translation="MQVVDRRHQLGVAVLRIAVGIIFLWAGLEKVIGGGGEWSAAGFL
                     GFGTGGTLGWPFVTGEPGEGEIFNPTNAFWVAVADNQTAMTVIDLLVPWGQIGIGVSL
                     ILGLLTRFGAAMGTLMMVMFFFAAWDFEFGIVNQHLTYAVVTFFLGYIGSGNFYGLDA
                     TVGERVSPTLRRWLFSGDLYRPVEGAPTPAPA"
     gene            7947..10718
                     /locus_tag="VGO32_00270"
     CDS             7947..10718
                     /locus_tag="VGO32_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7199219.1"
                     /translation="MTAHPGVVRPDRVATAPVQEAAATGVVPRPSTVTRRAALSMGLV
                     GTFALAGALGMAEAISWWNQGALTVASALVVLIVLEGRRNASPDDVPFQNALVPVVGL
                     WLVIQGLALFEQVTGAQVGSLIRFPLVLVFAIACAPIWRAASRGRLTGIEASAVYLDS
                     SIVFAAVAAGALLALGGTTLDGAQAIYGLLFAALFFGMIGAAAVLNLALTSVRAVGGW
                     IAAVTGLLIMGLGLSSYLAHADTIGWSPGIAAVSAGLIVTAYGGATWTRRIDPSEAYR
                     RRALQVREALPLLAAALSPLLWVLGEAMRDTVQREVSAAVAGSVAAVLILCVLRQTIL
                     LRERDRIVGLATSAAHRERVLHAELESSERRFRTLVTNSTDVVLIVGRDGRIAYQSPS
                     VERVLGHSPNSRLGHPVGVLAHPHDAEYFRSVLAELAALPGAERTLELRTRHADGSWR
                     ILEATAKNLLDDPAVAGIVINCRDVTERRALEEQLKHEALHDPLTTLANRALFLDRVG
                     HALSRRDKPDSTGVLFMDLDDFKTINDSLGHAAGDELLQAVAQRIRGNLRLEDTVARL
                     GGDEFAVLVEDADMEMVNQVGSRLLGALRAPFEIAGKQVHIGASVGAAMSGTDNRDAE
                     VLLRNADVAMYTAKSRGKGRLEIFEASMHAAAVTRLELKADLERALDRGEMRVRYQPI
                     FYLSDGALDGFETLVRWRHPDRGEVGPNDFIPLAEETGLIVPIGKWVLVQACRQAQAW
                     RLGGGDTVKVSVNLAARQLRDPEIVEMVAGVLAETGLEPDALTLELTESSIMQDDEGR
                     LQELRGLGIHLGLDDFGTGYSSLSYLSKFPIETLKIDRSFTSRLGGPDEETALVRSVI
                     QLATSMGMETVGEGIERPEQLERLRELGCTYGQGYLLARPMDAIKATTLATTGRMEAT
                     DPEATAAEA"
     gene            10757..>11749
                     /locus_tag="VGO32_00275"
     CDS             10757..>11749
                     /locus_tag="VGO32_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEV7199220.1"
                     /translation="MGYDYTKVKVESVEKETDAGLADADALVAQAVGADEVSFEARVR
                     PLELANARLVVAYGRGAFLGHAGVDAAVRDAGNAADERITKWRTALPFREDLYHAVRE
                     LAESPAAATLTPEQEHVLMLWMRDFRRAGQALEPANRAELETIRARLVELEVAFNRNI
                     AEYQDGIEVTREELAGTPDDFVERLTAGAQPDTFRVSLDYPELYPFLSYASNRARRQE
                     LYTKHWSRAVDVNRPLIEEALRLRQRAAELLGFPTWAHFAIEVKMAETPDAVAAFYDQ
                     IVPGLAAIRDRELLVLAERLRGDGYAGEVTASDWAYYDEQLKRDEFGVDANLVAE"
BASE COUNT         1855 a         4027 c         4022 g         1845 t
ORIGIN      
        1 catcccgtcc agcaggctga actccgagtg cacgtggagg tgcacgaacg agtcgccagt
       61 tcccgcgggt ggcatcctac cgatactagt cggccccgcc gcccgtggtt cggggatgcc
      121 gcgccggtca ggtcacacca ggggaatcca acttgacaag atccctcata caaccgtatg
      181 gttgtactaa tgcagatcgt tcctccagac ttcgaccgca tgttcggcgc cctgggcgat
      241 gccacgcgtc gggacatcgt gcgtcgcgca atcgacggtg aggagggcgt cgccgagctg
      301 gccacccact accccatgag cttcgcggcc gtccagaagc acgtcgcgat cctcgagcgg
      361 gcgggcctgg tcaccaagga acgcttcgga cgacggaagg tcgtccgcgc caacctcgac
      421 gggctgctca tggcgcggcg actgctggac cgatacgagg agctgtggct cgggcggatc
      481 gaccgcatga ccgagctcgt caccgagaac ccaaaggaga ccaaccgatg accgttacgt
      541 ccgtccgcaa ggacctcgaa gccctgaccc tgacccttga tgccgagttc gatgcctcgg
      601 cccagagggt ctggcagctg tggtccgatc cccgccagct cgagcgctgg tggggtccgc
      661 cgacctatcc ggccaccttc accaagcatg acctcgcgcc cggcagtcac gtcgaatacc
      721 acatgaccgg gcccagcggc gaccagccgc gcggctactg ggacatcgtc gaggtcgcgc
      781 ctccgcatcg gctggtcttc ctcgacggct ttgccaacga ggacgggacg ccgaacgccg
      841 atatgccggt gaacacggcg ctggtgacca tccaggagat cggcaacggc cgcacgcgga
      901 tgtcgatcga gagccgctac ccgagcaccg ccgcgatgga gcagaacctg gccatgggga
      961 tggagcaggg cctgaccgag gcggttggtc agatcgacgc catcctggcc gagagcgctg
     1021 tcccgcaagc ctgactggaa ccacgaggaa tacacgcatg accgcaacga cgatcgagcc
     1081 gaccactagg accctgccgg cgctgggtgc cgtcatcacc tacgacatcc ggcccaacgc
     1141 cgagaccacc gagcccatcc tcttcctaat cgggtcaccg atgggcgcgg gggggtttgg
     1201 cacgctggcg ggcaacttca ccgatcggac ggtcgtgacc tacgacccgc gtgggtcgga
     1261 acggagtgtc aaggacgacc ccacgaccga gtccacgccc gaccagcacg gggacgacct
     1321 ccatcgggtg atcgaagcgc tgggtgccgg accggtcgac ctcttcgcga gcagtggtgg
     1381 ggcggtcaac gccctggcgc tcgtggccgc gcatcccgag aatgttcggc tgctcgtcgc
     1441 ccatgagccc ccggccgccc aggtgctgcc cgatcgcgag gcggcgctgg ccacgacccg
     1501 cgccatccac gagacctacc agaagggtgg ctggggcgcc gggatggcgc atttcatcgc
     1561 cgtggtgggt cacaagggtc ccttcccgga tggctatgcc gaccagcccg ggcctgaccc
     1621 ggcgacgttc gggatgccca ccgaggacag cggcgaccgg accgatgtga tgctgggtca
     1681 gaacatcatc acctgcaccc actaccagcc cgacttcgat gcgctgcgag ccgcgtcgac
     1741 ccggatcgtg atcgcggccg ggatcggctc ggagggcgag atggccaatc gggcggccca
     1801 tgccgtcgcc gagcggctgg gaaccgagct ggtcgacttc cccagcgacc acggcgggtt
     1861 cctgggcggc gagtacgggc agaccggcga cccggatgcc tttgcggcca agctgcgcga
     1921 ggtccttgcc accgaggtct gatcaccaac ggtcagttca gcggatcacc gtctcccgcc
     1981 tggaacagtc caagggcccc gcgcccaacg aagttgaagg cgtgggtcac catcgggtat
     2041 aggccgtctt cgggcatgac catctcgatg atcgccccct gggccggtga cagatccacc
     2101 gcctgcgacc cgaagttccc cgggttgccg ggtgtcaggt ggaccccctc cttgatcacg
     2161 ctgctgaaga tcgtgcccac cacgtggaag ctgctgtcaa tgcttggccc ggcattgagg
     2221 acgaatacac ggacccggtc tccggtcccg accgcgattg ggttgtccag gtattggttc
     2281 gccacgccgt tccacacgac gtagtccggc gcgggagccg ctgcggccgc cttggtcaag
     2341 ctggccggct cgccctgggg tccgagatac cactcgctct gtacgaccgc gatctcggca
     2401 tccaccggag gcaggccttc ggccggctcg acgatgacca tcccgtacat cccgttcgcg
     2461 atgtggtgga gggccggcga cgtcccgcag tggtacatcc acacgcccgc atagtcggcg
     2521 gtccattcgt agaccttctc ctcgcccgga ttgatgctgg tcatctcgtc gttccaggcg
     2581 acctgcgagg catggaagtc gatcgagtgc gcaaccgcac tggtctcggt attgaccaga
     2641 tgaacccgga tcgtgtcgcc gacccggacc cggatcacgg ggccgggaac ggttccgttg
     2701 aaggtccaga ccgcctgcac gaatccgccg tcgtccccct gggcgacggt catcggcttc
     2761 tcctcgatga ccagctcgat gtcatgcacg gttccctcga gccgcggcgg cgcttcggct
     2821 gggtacagca cgtacggagg agcatcgggg tcgggctcga cgtcgctatc gggtgccggt
     2881 ccgccgtgat cgtcaccacc tgtcgggggg cccaccccgg caacggtgac gatgcccgtc
     2941 attcccccgc tcgcgtgccc aggcaccgag cacaggaacg tcgtcccgcc agccggaatc
     3001 gtcacgtcga acatgatcat ttctcccggt tccgccgact gcgcggtcgc gccctcgaag
     3061 gtgatgtcgt gcagcacggc tccgtcattc atgaaatgga ccgtgtatct gcctggctcg
     3121 gcgacatcga ccgtcgacgg ctcgaagccg aggtcaaacg cgctgatggt gatttcgcca
     3181 acgatctccc cggactgggc tgggcttgcc aaggatcctg gagcctgctc tgcggcggcg
     3241 aggatcgcga agatcagcga gagcaggatg acgctggtaa caccggcggc gtagaggaag
     3301 agacggccca tgtcgtggat ctccttgact tcagggggac tgagacgggt ttcggtgtga
     3361 attgaggact tggccgggac gcagtggcag gccgggtccg ccgtcgctgc ccgaagcggc
     3421 ccgggcgagc tcgccaagga gccaatctgc cgtgtaccgc gcagcgcggt gcatcagggc
     3481 ccgatcagcc accgagccca gcgcgcctcc gggcgggacg tatcggccgt ctatcgcgat
     3541 ctcgccgggt cgtgcggtga gccagccgga gaagaccggg aagagcgggg ccagtgatgc
     3601 cgaccgccag ctgacctccg cccgcagggt ctggtcggtg cggtcgaggg atccgatgtc
     3661 gacgtaggcg gacttatgaa ccgtcagccg ggtcccgatc cggtggcccg ccttgaggtc
     3721 ggtcgcgtat cggcgcctgc ccgctggtcc ggcggagatg gcgtcgccga gccatgcgtc
     3781 gggaccgccc gcgtcgagca gtcggccgat gtcgtcgacc tccagccctg tgcgcaggct
     3841 tacctggagt cgcgtggaac cgccggtctt gaccatccgc ccaaggctgc gtgcttcgcg
     3901 gtgggcgcgg ataggtgcga gtggcccgta tccacgggcc gttcggccgg tgacgggcag
     3961 cttctgcagg ggcaccgttg atgctgagct taacttcccg tccggggacg attgaaaggc
     4021 aggatggcga tgacacggat cctggtgacg acagcctcga agcacggatc gactcgcgag
     4081 atcggggacg cgatcgcggc ggtcctcgag tccatgggga tcgaggcaat cgcaaaggac
     4141 gtcgatcagg tcgcgaccgt cgaggacgtt gacggcgtga tcgtggggag cggcgtgtac
     4201 gcgggccact ggctcgcgtc cgccacggcg ttcgtcgacc gcttccaggt cgagctccag
     4261 caacgcccgg tgtggctctt ctcgagcggc ccgctcggcg accccgcgcg acccgcggag
     4321 gatccggccg aggtgccgac ggcgatggcg tcctccggcg cgctcggcca tcgcctcctc
     4381 gcgggtcggc tggagaagac ggatctgggt ttcgcggaac gggcaatcgt caacgtcgtg
     4441 cgagccccgt acggcgattt ccgcgactgg ccggagatcc gaggctgggc actcgagatc
     4501 gcgggcacgt tcacgcgggt gcgtgcgaat gcaggatgag ccgcgttcag tcgccgggga
     4561 gccaggtcgg gttccggacg caccctggat cgtgtgcggc cgctcgactc gtgacattct
     4621 cgacggctgg gtacgctgtc cgcagcaggg aatgctcccc gtaaatgcct gcctggagtg
     4681 ccacctcctg gtcacgatca cgggcgagcg ggatccgcgg aaagcgtgtt cgacagaggt
     4741 atgaccgatt ctcgacgggg cgaccagatt gccgccgatc atggaatcgc gaaggagaca
     4801 ggcatggaaa cttcacggag ccgggtggcc gtcatcgacg aggcacgaag caatgacgca
     4861 ttccggcgcg tcctgatcac cgggatctac gagcaggtcg tcgtcatggc gatcccgccg
     4921 ggcggcgaga tcggggacga ggtccacccg gagactgacc aggtactcgt gttcgtggaa
     4981 ggcgaggccg aagcccggct cgacggcgag atcacccgtg tcggcggaaa cgacctggtg
     5041 tacgtccggg ccggaacgcg acataacttc gtcaacaccg gcccatcgcc gcttcggctg
     5101 tacaccatct acgcgcctcc tcatcacccc ccgggcacgg tgcatcagac gaaggccgac
     5161 gctgacgctg cggactgagc tcggtgccgt gtccggcgta cccgactgag gcgtcctgct
     5221 accgtttcgg ccatgcacgg tgccgccgag gccgtcgaca agtctctccg cgagcgtgac
     5281 gcgctcgatg cggcggtgca gggcatcgcc ggcgtcctgg acctcgatcg cgtcctgcag
     5341 ctgatcgtcg atcgtgtccg ggagcttgca gacggcgagt acgccgcgct cgggatcatc
     5401 ggcccggacg aatccctcga gcgtttcatc accagtggca tcgacgccgc tgaacggaac
     5461 cggatcggcg ccctgcctac cggtcgcggc ctgctgggcg tgatcattcg cgaaagccag
     5521 tcgcttcggc ttgccgacct ggcgaccgat gagcggcgca gcgggttccc cgcccatcac
     5581 ccaccgatgc acagcttcct gggggtcccg gtgcggatcc gggatcgggc gatcggcaac
     5641 ttctacctca ccaacaagcg cggtgccgcc gagttcagcc tggacgacca gcgcctggtc
     5701 gagcgcttcg cgctccccgc cgcgatcgcg atcgacaccg ctcggctgca cggcgaggtc
     5761 cagcggctgg cgatcatggc cgatcgcgat cgaatcgggc gggacctgca tgacggggtc
     5821 atccagcgcc tgtatgcggt gacgctctcg ctcgacgatg tgcccgagct catggacgaa
     5881 gcaccgggag aagcacgcga gcgcgtcgag cgatcgatcc aggcgctgca gtccgcgatc
     5941 ggcgacatcc gagcattcat ctacgacctt cgaccggtgg aggtgtccgc caccgacctc
     6001 catggcgcgt tggtcgagct ggccgaggag gccgagatga acggcgcgac cgaggtccag
     6061 gtccagatcg acggcgacgc gcctattccg tccgcgcagg ccatcgagat gctggcgatt
     6121 gcccgcgaag cgctgagcaa tgtcgggcgc cacgccgcgg cacggcacgc gaccatccat
     6181 ctggcgatct ccggcgatgc cgcgcgcctc acgatcaccg atgacggtcg cggattcgac
     6241 gtcgaggcgc cacgcgacgg cgggcatcgc gggctcggga acatgcggga ccgggctggc
     6301 gccttgggcg gtcgcctcac ggtgacctcc gttgccggag aagggacccg cgtcgtcgcg
     6361 gaaattcccg ggcagtcatc agatccagtc gcatgacgaa tcccgagacc gcgggtccgt
     6421 ccgccgctcc cctgagactc ctgctcgtcg acgaccacga ggtcgtacgc cagggtctcc
     6481 gggccctcat cgagcgccgc ccggcgttcc aggtcgtggc ggaagccggc accgtggctg
     6541 acgcgatcgc cgcggcccgg catttcaagc ccgacctcgt gctcatggac gtccgcctgc
     6601 ccgacggaag cggtgtcgag gcctgtcggg ccatccgcga ggacaacccc gcgatcaggg
     6661 ttgtcatgct gaccagctac ccggacgaag aagcggtgct ggccgccatc ctggccggag
     6721 cgagtggata tctcctgaag gagattcgag gccgggacct ggtggccgcg ctcgagtcgg
     6781 tcggacgtgg tgactcgctg ctcgatccgg ccgtcaccga gcgcgtgctc gagcgcgttc
     6841 gtcggatggc ggccggcgat gcacaggacg agctcgccga gctgaccgcg caggaacgga
     6901 agatcctcat gctggttgcg gagggaaaga cgaatcgcga ggtcgccgcc gaggtttttc
     6961 tgtccgacaa gacggtgaag aactacgtct cgtccatcct ctccaagctc ggcctccaac
     7021 gccgggccca ggcagccgcc ttcgtggcga agcaccggct cggcgacggg acctgacgga
     7081 ctcgaatttc gtgactttcg gcacggaatc gccaagaatg gctggaatac actctcgtcg
     7141 gtaagacaag gcatcgggcc gttttcgaaa ggagaccttg gccatgcaag tcgtcgaccg
     7201 cagacaccaa ctcggagtag ccgtactccg gattgcggtg ggcatcatct tcctgtgggc
     7261 gggactggag aaggtcatcg gcggcggcgg tgaatggtcc gcggccggct tcttgggctt
     7321 cggcaccggt ggaacgctgg gctggccgtt cgtgaccggt gagccgggtg agggcgagat
     7381 cttcaacccg accaatgcgt tctgggtagc cgtggccgac aaccagaccg ccatgaccgt
     7441 gatcgacctt ctggttccct ggggtcagat cgggatcggc gtcagcctga tcctgggtct
     7501 gctcacccgg ttcggggcgg ccatggggac gctgatgatg gtgatgttct tcttcgccgc
     7561 gtgggacttc gagttcggga tcgtgaacca gcacctgacg tatgccgtgg tcacgttctt
     7621 cctgggctac atcgggtccg gcaacttcta cggcctcgac gcgacggtcg gcgagcgggt
     7681 ctcgccaacg ttgcgccgct ggctcttctc gggcgacctc taccgtccgg tcgagggggc
     7741 accgactccg gcgcctgcgt aacgcgaaca gccaccgcgg ggagatggcg cccggcaacg
     7801 ggcgccatcg gtatgcctga ggtcgagtcc tagtacccgg cagtcgccgc gggtggcccg
     7861 cggaactcgg cgctggctcg gcatgacccg atggtcccaa tgggtgatgg cctcggattg
     7921 gccccctccg tagcctccaa catcagatga ccgcgcatcc gggggtggtc cgacccgacc
     7981 gggtggcgac ggcccctgtg caggaggcgg ccgccaccgg tgtggtgccg cgcccgagca
     8041 ccgtcacccg ccgcgcagcc ctctcgatgg gcctggtggg cacattcgcg ctcgccggcg
     8101 cgctgggcat ggccgaggcg atctcgtggt ggaaccaggg ggcactgacc gtcgcctccg
     8161 cgctcgtcgt cctgatcgtg ctcgagggtc ggcggaatgc cagcccggac gatgtcccgt
     8221 tccagaacgc gctcgtgccg gtcgtcggcc tgtggctcgt gatccagggc ctcgccctgt
     8281 tcgagcaggt caccggcgcg caggtcggct cgctcatccg cttcccgttg gtgctcgtct
     8341 tcgccatcgc ctgtgcgccc atctggcgcg ccgcctcgcg cggccggctg accgggatcg
     8401 aggcgtcggc cgtgtacctg gactcgtcca tcgtgttcgc cgcggtcgcc gccggcgcgt
     8461 tgctggccct cggcggaacc acactcgacg gcgcccaggc catctacggg ctgctgttcg
     8521 cggccctgtt cttcgggatg atcggtgcgg ccgccgtcct gaacctcgcc ctcacctccg
     8581 tccgtgccgt gggcggctgg atcgccgccg tgacgggcct gttgatcatg ggactggggc
     8641 tctcctcgta tttggcccac gccgacacga tcggttggag tccgggaatc gccgcggtca
     8701 gcgctggcct gatcgtgacc gcctacggtg gcgcaacctg gacccgccgg atcgacccca
     8761 gcgaggcgta ccgccgccgg gccctccagg tccgcgaggc gctgccactg ctggccgcgg
     8821 cgctgtctcc cctgctctgg gtcctgggcg aggcgatgcg cgacaccgtc cagcgcgagg
     8881 tgagcgccgc ggtggccgga tccgtcgccg ccgtcctcat cctgtgcgtg ctgcgccaga
     8941 cgatcctcct ccgcgagcgt gaccggatcg tgggcctggc cacctcggcc gcgcaccgcg
     9001 agcgggtgct ccacgccgag ctggagtcga gcgaacgacg gttccgcacc ctggtcacga
     9061 actccaccga cgtggtcctg atcgtgggcc gggatggccg gatcgcatac cagagcccct
     9121 cggtcgagcg ggtgctgggc cactccccga actcgcgcct ggggcatccg gtgggcgtcc
     9181 tcgcccaccc gcacgacgcg gagtacttcc gcagcgtcct cgctgagctg gcggccctcc
     9241 ccggggcgga gcgcacgctc gagctccgga cccgccacgc agacgggtca tggcggatcc
     9301 tggaggccac ggccaagaac ctgctcgatg acccggcggt ggccggcatc gtcatcaact
     9361 gccgcgacgt gaccgagcgg cgcgcgctgg aagagcagct caagcacgag gctctccacg
     9421 acccactgac gaccctcgca aaccgagccc tgttcctgga ccgggtgggc cacgccctgt
     9481 cgcggcgcga caagcccgac tcgaccggcg tcctgttcat ggacctggat gacttcaaga
     9541 cgatcaacga cagcctgggc cacgccgccg gcgacgagct gctgcaggcc gttgcccagc
     9601 gcatccgcgg caacctccgg ctggaggaca ccgtcgcccg gctgggcggt gacgagttcg
     9661 cggtcctggt cgaggacgcg gacatggaga tggtcaacca ggtcggcagc cggctgctgg
     9721 gcgccctccg cgccccgttc gagatcgccg gcaagcaggt ccacattggg gccagcgtgg
     9781 gcgcggcgat gagcggcacc gacaatcgcg atgccgaggt cctgctccgc aacgcagacg
     9841 tggcaatgta caccgccaag tcccgcggca agggccggct cgagatcttc gaggccagca
     9901 tgcacgccgc ggcggtcacc cggcttgagc tgaaggccga cctcgagcgc gccctcgacc
     9961 gcggcgagat gcgggtccgc taccagccca tcttctatct gtccgacggc gcgctggacg
    10021 gcttcgagac cctcgtccgc tggcgccacc ccgaccgcgg cgaggtcgga cccaacgact
    10081 tcatcccgct cgccgaggag acgggcctga tcgtgcccat cggcaagtgg gtgctggtcc
    10141 aggcctgccg ccaggcccag gcatggcgcc tgggcggggg cgacacggtg aaggtcagcg
    10201 tcaacctggc cgcccgccag ctgcgggatc cggagatcgt cgagatggtg gccggcgtgc
    10261 tggccgagac cggcctcgag cccgacgcgc tcaccctgga gctcaccgag tcgagcatca
    10321 tgcaggacga tgagggccgg ctccaggagc tgcgtggcct gggcatccac ctgggcctgg
    10381 acgacttcgg caccggctac tcctcgctca gctacctgtc caagttcccg atcgagaccc
    10441 tgaagatcga ccgatcgttc acctcccgtc tcggcgggcc ggacgaggag accgcgctcg
    10501 tccgctcggt catccagctg gcgacctcga tggggatgga gaccgtgggt gaaggcatcg
    10561 agcgccccga gcagctggag cgcctccgcg agctcggctg cacgtacggg cagggctacc
    10621 tgctcgcccg gccgatggac gcgatcaagg ccacgaccct ggccaccact ggccggatgg
    10681 aggccaccga ccccgaggcg accgccgccg aggcctgacc ctatcggccc gtgagccccg
    10741 cgggtaccat ccgggcatgg gatacgacta caccaaggtc aaggtcgaat cggtcgagaa
    10801 ggagaccgat gccggcctgg ccgatgccga cgccctggtc gcccaggcgg tgggcgccga
    10861 tgaagtctcg ttcgaggctc gcgtccggcc gctcgagctc gccaacgccc gcctggtggt
    10921 ggcctacggc cgcggggcgt tccttgggca cgcgggcgtg gacgccgcgg tccgggacgc
    10981 cggcaacgcg gctgatgagc ggatcacgaa gtggcgcacc gccctcccgt tccgcgagga
    11041 cctgtaccac gcggtccgcg agctggccga gtcccccgcc gccgccacgc tgacccccga
    11101 gcaggagcac gtccttatgc tgtggatgcg cgacttccgt cgcgcggggc aggccctgga
    11161 gccggcgaat cgagccgagc tggagacgat ccgcgctcgc ctggtggagc tggaggtcgc
    11221 gttcaaccgg aacatcgccg agtaccagga cgggatcgag gtcacccgcg aggagctggc
    11281 cgggacgccg gacgacttcg tggaacgcct gaccgccggc gcccaaccgg acacgttccg
    11341 cgtcagcctg gattaccccg agctgtaccc gttcctgtca tacgcctcga accgcgcacg
    11401 acggcaggag ctgtacacca agcactggag ccgcgcggtg gacgtcaacc ggccgctcat
    11461 cgaggaggcg ctccggctcc gccagcgcgc ggcggagctg cttggcttcc cgacctgggc
    11521 ccacttcgcg atcgaggtga agatggccga gacgccggac gcggtggccg ccttctacga
    11581 ccagatcgtc ccgggcctgg ccgccatccg cgaccgcgag ctcttggtcc tcgccgagcg
    11641 gttgcgcggc gacgggtatg ccggcgaggt caccgcctcg gactgggcct actacgacga
    11701 gcagctcaag cgcgatgaat tcggcgtcga cgccaacctg gtcgccgag
//
LOCUS       DASYGU010000008         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_722352, whole genome shotgun sequence.
ACCESSION   DASYGU010000008 DASYGU010000000
VERSION     DASYGU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722352"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VGO32_00280"
     CDS             complement(<1..1182)
                     /locus_tag="VGO32_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV7199221.1"
                     /translation="MPRTRIPATSMQQDAALELARRVGERIRVARRQAGMTQSQLAGD
                     RYTKAYISALETGITRPSMVALNFVADRLGLPASHFLGDSHPTWTRLEVDMRLASGEW
                     AGAAEGYRALVGTELSDLSRAEVLRGLAEAEARQDHGREAIAAGSEAVRIFAGMGRHA
                     DEALARYWMAYGTYLTDNEADARTQLQGLLAQVRDGLQVEPEFEMRVLTALAAVESRV
                     GEHARSLAYLEEARGLADDLDDRRRATFLFNLAITYRETGDVEAAIRVGQQGLALYRA
                     AGAPFESATIENDLALAYLAMGNLDRATEMATEAHEQFEAAGDDRFLAAVLDTEAQIA
                     LAGGKHDRALALAARSHQLARETGNRLVETVALITAARVLRARGDRAGAEQRYKAAAE
                     LA"
     gene            complement(1509..4166)
                     /gene="ligD"
                     /locus_tag="VGO32_00285"
     CDS             complement(1509..4166)
                     /gene="ligD"
                     /locus_tag="VGO32_00285"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEV7199222.1"
                     /translation="MPLETYRSKRDFAKTPEPAGAAPIETSGRFVVQRHRATRLHYDL
                     RLEMNGVLASWAVPRGPTLDPDERRLAMKTEDHPIEYLEFEATIPKGEYGAGDMIVWD
                     WGRYEPEETDAPAAAVAAGELKFRLHGERLRGRFTLVKTRGRTDDQGRTEGGDPWLLI
                     HKRDEHAEAGFDAETQARSVKTGRTNDELAAGREPRFEAPPPAPEAPIDLSAAREAPL
                     PEFVSPMLATAGERPYEDESWFHEVKWDGYRVQAVVRDGKVKLWTRNRSDGASYFPEL
                     AGPPSWINAREAVVDGEVVAFGPDGRPSFSALQERTGLMGLESRTGRRRPRSTEDKKR
                     SSDRSSPAPILYQAFDLLHLDGTSLLDVPLESRKRLLQRVLRPHPLVHYATHVAGEGS
                     AFLDAALAGGLEGVVAKRRTSRYRPGERSPDWVKIKVRSEQDVAVIGWLPRQGSTSDI
                     GSLIVAVMGPDGLVHAGQVGSGLDARTRRELLAAFASISRSDPPAAHAASVPGGRWVE
                     PKIVARVEFAEWTPDGLLRQPTFLGVLVDRDVASTVREDPPRRARRDTPAAPVTPPGA
                     IAAVSDAELAALDAMDGEGQWEIGGQVVRLTNLDKILFPSAEGDDPETAPVTKRDLAR
                     YYVTVGNTLVPHLRRHGLTLQRFPDGVSRKGFWQKDLPSHTPAWISRRELQDSEGVKQ
                     YVVVDRIATLAWLAQEAAIELHPWTATIEAPDRPRFALIDIDPGEATTWEEVVVLARL
                     FRTALGHLGVIGLPKTTGKRGIQIWVPIADRYSFDDTRAWVEGLSRAVGQIVPDLVSW
                     AWSKRDRGGRARLDFTQNAPIRTLVAPYSVRPAPGAPVSAPITWDELDDPLLGPERWT
                     VRSLPARLTEMGDLFAPALSLRQDLPPLG"
     gene            complement(4170..4847)
                     /locus_tag="VGO32_00290"
     CDS             complement(4170..4847)
                     /locus_tag="VGO32_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV7199223.1"
                     /translation="MAPLEGTRPPRPGAVICDLDGTLVDTVPTRIDAWLRTFAEFGID
                     AERDHVASLIGSDGKWLAGQVAERTGRELAPDRAESIDQRAGEIYSELNTDPRPLPGV
                     PEFLAALGETRLAWAIATSSRPDQVRASVTALGLDPWPTVVDGLAVEQAKPEPDLLFA
                     AAARLEVPPATCWCVGDSRWDVLAASAAGMAPIAVATGASSATELGQFGATTVVERLD
                     QLIPYLA"
     gene            4868..6094
                     /locus_tag="VGO32_00295"
     CDS             4868..6094
                     /locus_tag="VGO32_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7199224.1"
                     /translation="MPSEFRALWPLDPAVDFMNHGSFGAAPTAVLEAQADIRRRMEAE
                     PVAFFSRDLEGLLAAARSALGEFVGAEPDDLAFTDNATTGVNAVLRWLPLGAGDELLT
                     TDHEYNACRNVLARAADATGARLVVVPLPFPTEGPSDVLERILAAVTPRTRIALIDHV
                     TSATALVLPIEELVAELTARGVDTLVDGAHAPGMLDLQLDELEAAYYVGNLHKWVCAP
                     KGSAFIHVRRDRQAGFHPLVVSHGANSDRTDVSRFRLESDWTGTRDPSAWLSVPTALE
                     FIGGLLPGGWSAVRLRNRDLALRGRDLLCAALAIASPAPDAMLGSMASVPLPEESAAG
                     RVQGVDLYGDPIHDGLMARGFQTMITPWPARPDDGPWRRVIRISAALHNDLDQFERLA
                     NALLEVLTEQPAAATA"
     gene            6123..>6442
                     /locus_tag="VGO32_00300"
     CDS             6123..>6442
                     /locus_tag="VGO32_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7199225.1"
                     /translation="MPLDLALLNPDPMVELARWVADAEAAGHRLPNAFALATADPTGR
                     PMVRMVLLTDVDADGLRFHTNRDSAKGRDMAANPRAAAVFWWEGTNRQVRVSGVVAPL
                     DDAES"
BASE COUNT          917 a         2330 c         2250 g          945 t
ORIGIN      
        1 cgccagctcg gcggccgcct tgtagcgttg ctcggcgccg gcgcgatccc cgcgggcgcg
       61 caggacgcgg gccgcggtga tgagagccac ggtctccacc agccggttgc cggtttcccg
      121 ggcgagctgg tgagaccggg ctgccagtgc cagggcacga tcgtgcttcc ccccggcgag
      181 cgcgatctgt gcctcggtgt cgaggacggc ggccaggaat cgatcgtcgc cggcagcctc
      241 gaactgctca tgggcttcgg tcgccatttc cgtggcccgg tcaaggttgc ccatggccag
      301 gtaggccagg gcgagatcat tttcaatcgt cgccgactcg aacggcgccc cggccgctcg
      361 atagagcgcc agcccctgct ggcccacccg gatcgcggcc tccacgtcac cggtctcgcg
      421 gtaggtgatc gcgaggttga acaggaaggt cgctcggcgc cgatcgtcaa gatcgtccgc
      481 caggccccgg gcctcctcga ggtaggccag tgagcgcgcg tgctccccga cccgtgactc
      541 aaccgccgcg agggcggtga gcacgcgcat ctcgaactcg ggctcgacct gaagcccatc
      601 gcggacctgg gccaggaggc cctgcagctg ggtgcgcgca tccgcttcgt tatcggtcag
      661 gtaggtgcca tacgccatcc agtagcgcgc gagcgcctca tcggcatggc ggcccatgcc
      721 ggcgaagatg cgcaccgctt cggatccggc ggcaatcgcc tcacggccgt ggtcctggcg
      781 ggcctccgct tcggccagtc cgcggaggac ctccgcgcgg gacaggtcgc tgagctcagt
      841 cccgaccagg gcacggtagc cctcggccgc cccggcccat tcgcccgatg ccagccgcat
      901 gtcgacctcc agccgggtcc aggtcggatg cgaatcgccg aggaaatggc tggcgggcag
      961 gccgagccga tccgcgacaa agttgagggc gaccatcgat gggcgcgtga taccggtctc
     1021 gagggcgctg atgtaggcct tcgtgtaccg atcaccggcc agctggctct gggtcatgcc
     1081 cgcctggcgg cgcgcgaccc ggatccgctc accaacacgc cgcgccaatt cgagcgcggc
     1141 gtcctgctgc atggaagtcg ccgggattcg ggttctcggc atcgggcgtg agtctacacc
     1201 tgaaaggcaa gcacaagttg acctcgaggt cgaccgaatc acgtcgccga aggtactccc
     1261 ccgaccgaga gtcacggtac ccacgggtca ttgcccgtgc ggacctccgg ctgcgagtgt
     1321 ctgggcacgt ggttctcact cgccagggtg agccgcgccg gacgggagac accacatgag
     1381 ctccgcgaca accgggggtg gttggccccg ttagaccgat gcacgcgcgg gcaccgcgca
     1441 caggtctcca ccactgatcc agggggagga cgccacggtt gcacatcggc cgtggcgtct
     1501 tcgtgtgttc agcccagcgg cggcagatcc tgtcgcaggc tgagcgccgg ggcgaacagg
     1561 tcgcccatct cggtcaatcg ggccggaaga cttcggaccg tccatcgctc gggcccgagg
     1621 agagggtcat ccagctcgtc ccaggtgatg ggcgccgaga ccggcgcccc aggagcgggg
     1681 cggacgctgt acggggcgac cagcgtccga atgggggcgt tctgggtgaa gtccagccgt
     1741 gcccgaccgc cgcggtcacg cttggaccat gcccacgaca cgaggtccgg cacgatctgg
     1801 ccgaccgccc gggacaggcc ctcgacccat gcccgcgtgt cgtcgaacga gtagcggtcc
     1861 gcgatcggaa cccagatctg gatcccccgc ttgccggttg tcttcggcaa gccgatgacg
     1921 ccgaggtggc cgagggctgt ccggaacaga cgggcgagca cgaccacctc ctcccaggtc
     1981 gtggcctccc ccggatcgat gtcgatgagc gcaaagcggg gccgatcggg tgcctcgatg
     2041 gtggcggtcc atggatggag ctcgatcgcc gcctcctggg ccagccaggc cagggtcgcg
     2101 atccgatcca cgacgacgta ctgcttgacc ccctcgctgt cctgcagctc gcgccggctg
     2161 atccaggccg gcgtatgcga tggcaggtcc ttctgccaga accccttgcg cgagaccccg
     2221 tccggaaacc gctggagcgt gagcccgtgg cggcggaggt ggggcaccag cgtattgccc
     2281 acggtcacgt agtagcgcgc gaggtcgcgc ttggtgaccg gtgcggtctc agggtcgtct
     2341 ccctcggccg acgggaacag gatcttgtcc aggttcgtca ggcgcacgac ctgcccgccg
     2401 atctcccatt ggccctcgcc gtccatcgcg tcgagggcgg cgagctcggc gtcgctcacg
     2461 gcggcaatcg ctccgggcgg cgtcacgggc gcggccgggg tgtcgcgtcg ggcgcggcgg
     2521 ggcggatcct cgcggaccgt tgaggccacg tcccggtcga cgaggacgcc gaggaaggtc
     2581 ggttggcgga ggagcccgtc cggcgtccac tccgcgaact cgacgcgggc cacgatcttc
     2641 ggctcgaccc atcgaccgcc cgggacggag gctgcatgag ccgcgggcgg atcggaacgg
     2701 gagatggagg cgaacgccgc cagcagctcg cgtcgagtcc tcgcgtcgag cccgctcccg
     2761 acctggcctg cgtggacgag gccgtccggg cccatgaccg ccacgatgag cgagccgata
     2821 tcactcgtgc tcccctgccg cggcagccag ccgatgacgg ccacgtcctg ctccgagcgg
     2881 accttgatct tgacccagtc cggcgaccgc tcgccggggc ggtatcggct cgtgcgccgc
     2941 ttggcgacga cgccctcgag accgccggcc agggcggcgt ccagaaaggc agacccctcg
     3001 cccgcgacat gggtcgcata gtggaccagc ggatgcgggc ggagcacgcg ttggagcagg
     3061 cgcttgcgtg actcgagcgg gacgtcgagg agcgacgtgc cgtcgagatg aagcaggtcg
     3121 aaggcctggt acaggatggg agcgggcgag gaccgatcgg acgaacgctt cttgtcctcc
     3181 gtcgatcgcg gtcggcgacg gcccgtgcgc gactcgaggc ccatgaggcc ggtccgctcc
     3241 tggagggcgg agaagctcgg ccgaccgtcg ggaccgaagg ccacgacctc gccgtcgacc
     3301 acagcctcac gggcgttgat ccatgatggc ggcccggcga gctcggggaa gtacgaagcc
     3361 ccgtcggacc ggtttcgggt ccagagcttg accttgccgt ctcggaccac ggcctggacc
     3421 cggtacccat cccacttcac ctcgtggaac cagctctcgt cctcgtacgg ccgctcgccg
     3481 gccgtggcca gcatcggcga cacgaactcg ggaagcggcg cctcgcgggc ggccgacagg
     3541 tcgatgggcg cctccggcgc gggaggcggg gcctcgaacc gcggctcccg cccggccgcg
     3601 agctcgtcgt tcgtccgtcc cgtcttcacg ctccgggcct gggtctcggc gtcgaacccg
     3661 gcctccgcgt gctcgtcgcg cttgtggatg aggagccatg gatccccgcc ctccgttcga
     3721 ccctggtcat cggtgcgacc ccgggttttg accagggtga agcggccgcg aagccgctct
     3781 ccgtggagcc ggaacttcag ctcgccggcg gccaccgcgg ccgcgggcgc atcggtctcc
     3841 tccggctcgt acctgcccca gtcccacacg atcatgtccc cggcgccgta ctcgcccttc
     3901 gggatcgtgg cctcgaactc gagatactcg atcgggtggt cctcggtctt catcgccagc
     3961 cggcgctcgt ctggatcgag ggtcgggccg cggggcacgg cccagctggc cagcaccccg
     4021 ttcatctcga gccggaggtc gtagtggagt cgcgtcgcgc ggtggcgctg gacgacgaac
     4081 cgaccgctgg tctcgatcgg cgccgcgccg gccggctccg gcgtcttcgc gaagtcgcgc
     4141 ttggaccgat acgtctccag gggcatcggt caggccagat acgggatgag ctggtcgagc
     4201 cgctccacga ccgtggtggc accgaactga cccagctcgg tggcgctcga ggcgccggtg
     4261 gccaccgcga tgggcgccat cccggccgcg ctggcggcca gcacgtccca gcgtgagtcg
     4321 cccacgcacc agcacgtcgc gggcggtacc tcgagccgtg cggcggcggc gaacaggagg
     4381 tcgggctcgg gcttcgcctg ctcgaccgcg aggccatcga ccacggttgg ccacggatcg
     4441 aggccgagcg ccgtgaccga cgcccggacc tggtccggtc gggaggacgt cgcaatcgcc
     4501 caggccagcc tcgtctcgcc gagcgcggcg aggaactcgg gcaccccggg caggggccgg
     4561 gggtcggtgt tgagctcgga gtagatctcc ccagcccgct ggtcgatgga ttcggcacgg
     4621 tccggcgcca gctcgcggcc ggtccgctcc gcgacctgcc cggcgagcca cttcccgtcc
     4681 gagccgatga gggacgccac gtggtcgcgc tcggcatcga tcccgaactc ggcaaaggtc
     4741 cgcagccagg cgtcgatccg ggtcgggacg gtgtccacca gggtcccgtc gaggtcgcag
     4801 atcacggcgc cggggcgcgg tggccgggtg ccttccagcg gagccatcgg tctacgatag
     4861 ccgccgcatg ccgtccgagt tccgcgcgct gtggccgctg gatcccgccg tcgacttcat
     4921 gaaccacggc tcgtttgggg ctgcccccac cgcggtgctc gaggcgcagg cggacatccg
     4981 ccggcgcatg gaagcggagc cggttgcctt cttctcgcgc gacctcgagg gcctcctggc
     5041 cgcggctcgg agcgcgctcg gcgagttcgt cggcgccgag cctgacgacc ttgccttcac
     5101 cgacaacgcc acgaccgggg tcaatgccgt cctccgctgg ctgcccctcg gcgccggaga
     5161 cgagctgctg accaccgacc acgagtacaa cgcctgccgc aacgtgctcg cacgtgccgc
     5221 agacgcaacc ggcgcgcgac tcgtggtcgt cccgctgccg ttccctaccg agggcccatc
     5281 cgacgtgctc gaacggatcc tggccgcggt cacgccgcgg acccggatcg cgctgatcga
     5341 tcacgtcacc tccgccaccg cgctcgtgct gcccatcgag gagctcgtgg ccgagctgac
     5401 cgcccgaggg gtggacacgc tggtcgacgg tgcccacgcg ccgggcatgc tcgacctcca
     5461 gctcgacgag ctcgaggccg cgtattacgt gggcaacctg cacaagtggg tctgcgcccc
     5521 gaaggggtcg gcgttcatcc acgtgcgccg tgatcgacag gccggattcc acccgctggt
     5581 cgtgagccat ggcgcgaact cggatcggac cgatgtgtct cgtttccgac tggagtccga
     5641 ctggaccggg acccgggacc cgagcgcgtg gctgtccgta cccaccgccc tcgagttcat
     5701 cggcgggctg ctccccggcg gctggtccgc cgtgcgcctg cgaaatcgcg atctcgcgct
     5761 ccgaggccgt gacctactct gcgccgccct cgcgatcgcg tcccccgccc ccgacgcgat
     5821 gctgggctcg atggccagcg tcccgctccc cgaggaatcc gcagcggggc gcgtgcaagg
     5881 cgtggacctg tacggcgatc cgatccacga cggcctcatg gcgcggggct tccagaccat
     5941 gatcaccccg tggcctgctc ggccggacga cggcccgtgg cgacgcgtga tccggatcag
     6001 cgccgccctg cacaacgacc tcgaccagtt cgagcggctc gccaacgccc tgctcgaagt
     6061 cctcaccgag cagcccgccg cggccacggc ctgagccgcg agccttccga cgccacccga
     6121 cgatgcccct ggacctggcg ctcctcaatc ccgacccgat ggtcgagctc gcccgctggg
     6181 tcgccgatgc cgaagccgcc ggccaccggt tgcccaacgc atttgcgctg gccaccgccg
     6241 acccgaccgg ccgacccatg gtccggatgg tgctcctcac cgacgtggat gccgacggac
     6301 tccggttcca caccaaccgg gacagcgcga agggccggga catggccgcg aacccccgcg
     6361 cggcggccgt cttctggtgg gagggaacga accgccaggt gcgggtcagc ggcgtggtcg
     6421 ccccgctgga cgacgccgaa tc
//
LOCUS       DASYGU010000009         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1648454, whole genome shotgun sequence.
ACCESSION   DASYGU010000009 DASYGU010000000
VERSION     DASYGU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648454"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2356
                     /locus_tag="VGO32_00305"
     CDS             <1..2356
                     /locus_tag="VGO32_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEV7199226.1"
                     /translation="IWSVDAEGAFSPAGEKQAFEQVMDLFAARLWQYPDAHIYHFAPY
                     EPTALKRLMGRHATREDAVDRLLRGAAMVDLYRISRQAFRASVESYSIKKLEPLYGFA
                     RDVALRDATSSIVVFEEWLELSEGDRPAADHLERIERYNHDDVASTRQLRDWLEERRV
                     ELATRTAQVIPRKTVQDPTPSPTLNARQETVAALAARLLRDVPETERTPAEQGRWLLA
                     QLLSWHRRENKSAWWLYFHLKNDLTDAERIEANEPLAGLEFDGEVRKEKRSTVYRYRF
                     PRQEYDIKVGGSVEDPATGLSPGEVVAIDDVNCTIELKHAGGKPHPTAIIPADDYQTG
                     VMEDALLRLGGWVADHGVEADGPHRAALDLLLRLPPRVAPEHGLPLRAVDEGGLEAAR
                     RLAAVLDRSTLAIQGPPGSGKTYTGARMALDLVRRGRRIGIMSNSHKVIGNFLTTVIE
                     AADEIQTTVPIIQKVSKLPDGLVHPSVTVTDQNPDVIAALHAGAVVVAGTAWLWSRED
                     MASAVDVLFVDEAGQVALANVLASAAGAASLVLLGDPQQLDQPTQGAHPPGADRSALA
                     HLLDDHSTMPDHLGLFLDTTWRLHPDLCDYTSEVFYEDRLEPEAGLGVQVVRGPPPMA
                     GTGPRLVLVRHQGNDNDSVEEADAVASLGRSLVEGGGTWVNEDGVERPVGWDDVLIVA
                     AYNAQVGEIRKRLPDRARVGTVDKFQGQEAPIAIYSMASSSVEDAPRGMKFLYSRNRL
                     NVATSRARCVALVVASPELLRVRARTPEEMRLANALARFAEMAR"
     gene            complement(2366..3247)
                     /locus_tag="VGO32_00310"
     CDS             complement(2366..3247)
                     /locus_tag="VGO32_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEV7199227.1"
                     /translation="MTERWVCPRCYTSAEESATVCPNCGTPRGAALASATGSSGAEHE
                     AGPPPEATPPESAPPPDAATPQPDPSSPTTASASGERWVCLRCFVSNEGLAAECANCG
                     LLRGAEPPADDPGTWAAAQQAQAAGGGRGFPWRWVGFGVVALLVLGGSILFAARRDDS
                     GEITGAGTLSVQDLQVGDCFDLEDERTADTTEVSTVRAIPCDQPHTYEMYFAGDYPAG
                     ELPTAPDEPYSEWEEGSCVGSFESYVGIDFDSSRWYFSSLTPTDESWDAGDRLLQCFL
                     HNLEETAVTGSAEGTAE"
     gene            complement(3244..3822)
                     /locus_tag="VGO32_00315"
     CDS             complement(3244..3822)
                     /locus_tag="VGO32_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEV7199228.1"
                     /translation="MADDQEPARDPPIHVDWVDQPLVGDDLPGRLGVTILPGKRGKSI
                     RYPGLVYRRDARTDLDRLRRLGVGLLVLLVEDHELERWGDPQLEDHAAAAGLRVRRLP
                     IPDGAAPPSVTAVDRLLAEMTAARATEDVVIACMGGVGRSGTIAACALVAAGQTPDGA
                     IAHLRVVRHPTAVETREQVAFVATYAQHVENR"
BASE COUNT          627 a         1426 c         1422 g          561 t
ORIGIN      
        1 gatctggtcg gtggatgccg aaggcgcctt cagcccggcc ggcgagaagc aggccttcga
       61 gcaggtgatg gacctcttcg ccgcgcgcct gtggcagtac cccgatgccc atatctacca
      121 cttcgcgccg tacgagccca ccgcgctcaa gcggctgatg ggccgccacg cgacgcgcga
      181 ggatgcggtg gaccgcctcc tccggggcgc agcgatggtc gacctctatc gcatctcgcg
      241 ccaggcgttc cgggcatccg tcgagagcta cagcatcaag aagctcgaac cgctgtacgg
      301 attcgcgcga gacgtcgcgc tgcgcgacgc aacctcgagc atcgtcgtct tcgaggaatg
      361 gctcgagctg agcgaggggg atcggccggc ggcggaccac ctcgagcgca tcgagcggta
      421 caaccatgac gacgtggcca gcacccggca gctgcgggac tggctcgagg agcgccgcgt
      481 cgagctcgcg accaggaccg cgcaggtgat cccgcgcaag acggtccagg accccacgcc
      541 cagcccgacg ctcaacgccc ggcaggaaac ggtggcggcc ctcgccgcgc gtctcctccg
      601 cgacgtgccg gagacggaac ggacaccggc ggagcagggt cgctggctcc tcgcccagct
      661 gctgtcgtgg catcggcgcg agaacaagag cgcgtggtgg ctctacttcc acctcaagaa
      721 cgacctcacc gatgccgaac ggatcgaggc caatgaaccg ctcgccggcc tcgagttcga
      781 cggggaggtt cggaaggaga agcgctcgac cgtgtaccga tatcggttcc cccgccagga
      841 atacgacatc aaggtcggtg ggtcggtcga ggacccggcc accggcctga gcccgggtga
      901 ggtggtcgcg atcgacgacg tgaactgcac gatcgagctc aagcacgccg gcggcaagcc
      961 ccacccgacc gcgatcattc cggccgacga ctaccagacc ggggtcatgg aggacgcact
     1021 cctccgcctc ggcggatggg tcgcggacca cggcgtcgag gccgatggac cgcatcgggc
     1081 ggccctcgac ctgctgctcc ggctgccgcc ccgggtggcg ccagagcacg ggcttccgct
     1141 gcgcgcggtg gacgagggtg gcctcgaggc cgcgcgacga ctcgccgccg tcctggatcg
     1201 gtccacgctc gcgatccagg gcccgccggg atcgggcaag acgtacaccg gagcccgaat
     1261 ggcgctggac ctcgtgcgtc gcggccggcg gatcgggatc atgtccaaca gccacaaggt
     1321 catcggcaac ttcctgacca cggtcatcga ggcggcggac gagatccaga ccacggtccc
     1381 gatcatccag aaggtcagca agctgcccga cggtctcgtc cacccatcgg tcacggtcac
     1441 cgatcagaac cccgacgtca tcgccgcgct gcacgccggc gctgtggtcg tcgccgggac
     1501 ggcgtggctc tggtcgcgcg aggacatggc cagcgccgtc gacgtcttgt tcgtggacga
     1561 agcgggccag gtggcgctgg cgaacgtgct cgcctcggcc gccggagcag cctcgctcgt
     1621 gctcctcggt gatccgcagc agctggacca gcccacccag ggggcgcatc cgcccggcgc
     1681 cgatcggtcg gccctggccc acctgctcga cgaccactcg acgatgcccg accacctcgg
     1741 cctgttcctc gacacgacat ggcggcttca tccggacctg tgcgactaca cctcggaggt
     1801 cttctacgag gatcggctcg aaccggaggc cggcctcggc gtgcaggtcg tccgcggacc
     1861 gccgccgatg gcaggaaccg ggccacgcct cgtcctcgtc cggcaccagg gcaacgacaa
     1921 cgactcggtc gaggaggccg atgcggtggc gtccctcggg cggtcgctgg tggagggtgg
     1981 aggaacctgg gtgaacgagg acggcgtgga gcggccggtc ggctgggacg acgtgctcat
     2041 cgtggccgcg tacaacgcgc aggtcggcga gatccgcaaa cgcctgccgg acagggcccg
     2101 ggtggggacg gtcgacaagt tccagggcca ggaggcgccg atcgcgatct actcgatggc
     2161 gtcgtcatcg gtcgaggacg cgccgcgggg catgaagttc ctctacagcc gcaaccgtct
     2221 caacgtcgcg acctcgcgcg cgcgctgcgt cgcgctggtc gtggcgtcgc cggagctgct
     2281 ccgggtgcgg gctcgcacgc ccgaggaaat gcgactcgcc aacgcgttgg cgcggttcgc
     2341 cgagatggcg cgctaagccg cctcgctact cggccgtgcc ctccgccgac ccggtgaccg
     2401 ctgtctcctc gaggttgtgg aggaagcact ggagcaggcg atcaccggca tcccacgact
     2461 cgtcggttgg ggtcagcgag ctgaagtacc accgcgagga gtcgaagtcg atcccaacgt
     2521 aggactcgaa cgatccgacg cacgaaccct cctcccactc gctgtagggc tcatccgggg
     2581 cggtcggcag ctcgccggcg gggtagtcac cggcgaagta catctcatat gtgtggggct
     2641 ggtcgcacgg gatggcgcgt acggtcgaga cctcggtcgt atcggcggtc cgctcgtcct
     2701 cgagatcgaa gcagtctccg acctggaggt cctggacgct gagcgtgccg gcgccggtga
     2761 tctcgccact gtcgtcacgc cgggctgcga acaggatcga gccaccgagg acgaggaggg
     2821 cgacgacccc gaagccgacc cagcgccacg ggaatcctcg gccaccgccg gcggcctgtg
     2881 cctgctgcgc cgcggcccag gtcccgggat cgtcggccgg aggctccgcc ccacggagca
     2941 ggccgcagtt cgcgcattcc gccgccaatc cctcgttcga gacgaagcag cgcaggcaga
     3001 cccagcgctc gcccgaggcg gatgccgtgg ttggcgatga cggatcgggc tggggtgttg
     3061 cggcgtccgg tggcggcgcc gactcgggtg gagtcgcctc aggcggcggg cccgcctcgt
     3121 gctcggcgcc ggacgaaccc gtggcagacg cgagtgccgc gccgcggggc gttccgcaat
     3181 tggggcacac cgtggccgac tcctcggcgg aggtgtagca ccggggacag acccaacgct
     3241 cggtcatcgg ttctccacat gctgggcata ggtcgccaca aacgcgacct gctccctggt
     3301 ttcaaccgcg gtagggtggc gaaccacccg gaggtgggca atagccccat cgggtgtctg
     3361 gccggcggcc acgagcgcgc aggccgcaat cgtgccggat cggccgacgc cgcccatgca
     3421 ggcgatgacc acgtcctccg tcgcgcgcgc cgcggtcatc tcggccagca gcctgtccac
     3481 cgcggtcacc gatggcggcg cggcgccatc gggaatcggc agccgtcgga cccggaggcc
     3541 ggcggccgcg gcgtgatcct caagctgggg atccccccag cgctcgagct cgtggtcttc
     3601 caccaggagg accagcaggc ccacgcccag cctccgcaaa cggtcgagat cggtccgggc
     3661 gtcccggcgg tagaccaggc ccgggtagcg aatcgacttc ccgcgcttgc cgggcaggat
     3721 agtcaccccc agccgccccg gcagatcgtc gcccaccagc ggctggtcga cccagtcgac
     3781 gtggatcggc ggatcccgag cgggctcctg gtcatcggcc atgccgggat cgtaccgggc
     3841 actccgcgta gagtggaggc cgatgagccg ccgacctcgc acccaggccg tgccgcggac
     3901 ggacgggtcc cgatcgacgc tccggccccg gctccccgag ggcgggatcc gcctacggtg
     3961 gctcgggcgc ggctcgacca tcttcctcgc ggctgggctg gtcctgatcg tggtaggcct
     4021 ggcgtccgtc gtggcc
//
LOCUS       DASYGU010000010         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1597343, whole genome shotgun sequence.
ACCESSION   DASYGU010000010 DASYGU010000000
VERSION     DASYGU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597343"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..2214)
                     /locus_tag="VGO32_00320"
     CDS             complement(193..2214)
                     /locus_tag="VGO32_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7199229.1"
                     /translation="MADRPDELRPITALFADISGSTGLGERLAPDEVKALIGECVTRM
                     SRAVEEYGGTVQAYQGDGICAYFGVPVAHEDDPERAARAALRIVELMAAYGREVEAAW
                     GIAELGVRVGVNSGRAGVGLVGSGDPQVVALGDATNVAARLESQAVPGTIVVGEETAR
                     RLAGRFVTEPLGEIMVKGRAEAVATWRLVGPEPVGEVPVSQPLVGREAEMGRLNAMLD
                     RLVSGQGQVTLVVGEAGIGKTRLLAELRGRAENRVTWLEGHSRSYGARSPFGPFVEIL
                     RQWLHDEGGPELAVRTRLRARLGADGARLLPELARLLGIRLDPEGDGDLADLPPADLG
                     ARLRSAYVEWLRLVGAGGPACVVIEDVNWADDSSADLAADVMALTDRAAIGLIVTLRP
                     DPATRGWGLRGQALTEYSHRLTTLDLEPLSVAATEELAALLLPGEGLDPATRRTLGEV
                     SEGNPLFLEELVARLQETGALAADHHAWTLTVPRQVLPPALEGLLVARIDYLSPEVRA
                     VAQAAAVLGREFTRRTLERMIIGDGTLDASLDALIRGGVIVERGRFPAPSYAFRHGLI
                     LEAVLSTLTDARRAELHLAAARAYEEGDAADIEAHLEELAHHYARANDYGKALTYLER
                     GAERAAELGATDEAGRLWLRALRAANKIEDGEAAERIRTRISNAPADKA"
BASE COUNT          315 a          883 c          839 g          333 t
ORIGIN      
        1 tcggtcgcgg tcggcgcgga cggagtgtgg gtcgccaacg cggcgggcgg atcggtgact
       61 cgcatcgacc ccgcgacgaa cgaagtggcg accacgatca cgatcggcgg ggagccggtg
      121 ggggtcgcgg tttcgggaac cacggtgtgg gtcgccgtcg cggcgccctg atccagcgct
      181 ggtctcgccc gactacgcct tgtccgccgg tgcgttgctg atcctggtcc ggatccgttc
      241 ggcggcctcg ccgtcctcga tcttgttggc ggctcgcaat gcccggagcc agagccgccc
      301 cgcctcgtcg gtggctccca gctccgccgc tcgttcggca ccgcgttcga gataggtgag
      361 cgccttgccg tagtcgttgg cccgcgcgta gtggtgggcc agctcctcga gatgggcttc
      421 gatgtccgcc gcgtcgccct cctcgtacgc ccgggccgcg gcgaggtgga gctccgcccg
      481 ccgcgcatcg gtcagcgtgc tgaggacggc ctccaggatg aggccgtgac ggaatgcgta
      541 tgacggagcc gggaagcgcc cgcgttcgac gatcacgcct ccccggatga gggcatccag
      601 gctggcatcc agggtgccgt ccccgatgat catccgttcc agggtccggc gggtgaactc
      661 acgacccagg acggccgccg cctgggcgac cgcgcgcacc tcgggcgaca ggtagtcgat
      721 cctggccacc agcagcccct cgagcgcggg cggcaggacc tggcgaggga cggtcaaggt
      781 ccatgcgtgg tggtcggcgg ccagcgcccc ggtctcctgc aggcgggcga cgagctcctc
      841 caggaacagg ggattgccct cgctgacctc gcccagggtg cgccgggtgg ccggatcgag
      901 accctccccg ggaagcagca gcgcggccag ctcctcggtg gcggccacgc tgagcggctc
      961 caggtcgagc gtcgtcagtc gatggctgta ctcggtcaac gcctggcccc gcaggcccca
     1021 gccccgcgtg gctgggtccg gccgaagggt cacgatcaga ccgatcgcgg cacgatcggt
     1081 cagggccatg acgtcggccg ccaggtcggc cgacgagtcg tccgcccagt tcacgtcctc
     1141 gatgaccacg cacgcggggc cgccggcgcc aacgaggcgg agccattcca cgtacgcgga
     1201 ccggaggcgg gcgccgaggt ccgccggcgg gagatcggcg aggtccccgt ctccctcggg
     1261 gtcgagacga atgcccagca gccgggccag ctccggcagc agcctggcgc cgtccgcgcc
     1321 caatcgcgcg cggaggcggg tgcggaccgc gagctccggt ccgccttcgt cgtgcagcca
     1381 ctggcggagg atctcgacaa acggcccgaa tggtgatcgg gcgccgtacg agcggctatg
     1441 gccctcgagc caggtcacac ggttctccgc ccgcccacgg agctccgcga gcaatcgggt
     1501 cttgccgatg cccgcctccc cgaccaccag ggtgacctgt ccctggccgc tgacgaggcg
     1561 gtcgagcatc gcgttcaggc ggcccatctc ggcctcgcgt ccgaccagcg gctggctgac
     1621 gggcacctcg ccgaccggct ccgggccgac aagtcgccac gtcgccaccg cttcggcgcg
     1681 gcccttgacc atgatctcgc ccaatggctc ggtcacgaac cgtcccgcca ggcggcgggc
     1741 cgtctcctcc ccgaccacga tcgtgccggg aacggcctgc gactcgagcc gggcggcgac
     1801 gttcgtcgcg tcgccgaggg ccacgacctg ggggtcgccg gatccgacca gcccgacccc
     1861 ggcccgaccg ctgttgactc cgacccggac gccgagctcg gcaatgcccc acgcagcctc
     1921 gacctcacgc ccgtaggcgg ccatcagctc gacgatccgc agggcggccc gcgccgcccg
     1981 ctcggggtcg tcttcgtggg caaccgggac accgaagtac gcgcagatgc cgtcgccctg
     2041 gtacgcctgg accgtgccgc cgtattcctc gaccgcccgg ctcatgcggg tcacgcattc
     2101 gccgatgagc gccttcactt cgtccggagc gagccgttcg ccgaggcccg tggagccgga
     2161 gatatccgcg aagagcgcgg tgatggggcg cagctcgtcc ggtcgatccg ccatgcgcgg
     2221 atgctagtgc cccggctgga cctcggcacc ctcggcgagg atggaccgat acatctccgg
     2281 gtcgatgttg cccccgctga gcacgatcac ccgcgacccg cggaccggcc ccgcggcgcc
     2341 cgtcatgtgc gcggccatgc cggcggcgcc
//
LOCUS       DASYGU010000011         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_671762, whole genome shotgun sequence.
ACCESSION   DASYGU010000011 DASYGU010000000
VERSION     DASYGU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671762"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..1241
                     /locus_tag="VGO32_00325"
     CDS             567..1241
                     /locus_tag="VGO32_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEV7199230.1"
                     /translation="MTSTFDETTALGAWPGPAAARDWFARQAGASDSSGRGTYDAGRT
                     VTEHDGVVDVFIHSEGDTRYVAALVAVIGDTVGQRITICMRADRIPGYKLAFLLWPSQ
                     GDGNDRGEIDFPEGKFEIGATAHAFMHYDGAAAQDGWDTGVSTADWHVYTIEWNPGSA
                     ASQSDDVCAFYLDGQLVGRSTGAPVPDVPMHYVMQMETFLAGQALPAPASGHVLIDWI
                     TIATPG"
     gene            complement(1238..1834)
                     /locus_tag="VGO32_00330"
     CDS             complement(1238..1834)
                     /locus_tag="VGO32_00330"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV7199231.1"
                     /translation="MFYTSGWVHVICGCMFCGKTDEMLRLLRRFAIAGRQVVLIKPRV
                     DTRTAEEAVVSRSGAQHRAVSVEGSADIEAMVGNADIVAIEEGQFWDADLPAVVERLA
                     EAGKQVLVTGLDQDYRGDPFGPMPMLMALADQVTKLTAICMVCGEPATRTQRLIDGLP
                     APADSPLIMVGGMGDEKYEARCRLHHEVPVADATAQSG"
     gene            complement(1856..2731)
                     /locus_tag="VGO32_00335"
     CDS             complement(1856..2731)
                     /locus_tag="VGO32_00335"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199232.1"
                     /translation="MTRLLIIGSPVAPGPAEAVQRAALAAAGRDVEIERWERRAHQLS
                     DALGELVQPDVMGAMIGSPHKEKAASLIGLLADEARASGAVNAVVNRDGRLHGYNTDA
                     AGVRAGLESILPESASRWPNQAVVLGAGGGARAVVSVLLEAGLGRIAVFNRHLHRAEA
                     VVAHFDRAARQTDLRALPWHETIIEAELSKAPLLVNASGIGAETEESPVPADLLAHDL
                     FVLDLVLNRAETPLMREAAARGGTVANGQGSFLVAQAAAFQLWTGEEPPADVMKTALA
                     AHLGLPEEGLAVVGD"
     gene            2843..2934
                     /locus_tag="VGO32_00340"
     tRNA            2843..2934
                     /locus_tag="VGO32_00340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2877..2879,aa:Ser,seq:gct)
     gene            2946..3021
                     /locus_tag="VGO32_00345"
     tRNA            2946..3021
                     /locus_tag="VGO32_00345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2979..2981,aa:Arg,seq:gcg)
BASE COUNT          471 a         1083 c         1011 g          465 t
ORIGIN      
        1 ccttcgcgtt ccctcggact gcccagatgg ccagcccgcc acaacaccga agctccggcc
       61 cagccgttcc cgacgaccgg gccatccgcg aaggaacgcc gcccatgcag gacatccgac
      121 ctcgacgcct gctcaacgcc gtattggcgg gtaccctcgt ggccgtcgcg gtggcgacgt
      181 atgcggctgc ggacgatgca cccctcggga ccaccctcgg ccgaccggac agcctccttc
      241 ccgcgcccgc atcggcacca cccggtatcg cgatggatgg ttcgccgtca cacgctccgt
      301 cgcgaggcag cgcttcggtt cgcacgccaa tcgggccatg gctgggcgaa tcaaccacgt
      361 cttccgcccc ggagcccacc tcccacgatt ggccgactcg agccgatatc ggcaccgccg
      421 acgagtcggc ggccccgacc gaaccggtaa cgccgacctc ggaacccccg atcgatgtcc
      481 cattccagcc gcagccaccg cccccgaccg cgatgagcgc gagctgcgcc gcggttccgg
      541 gcgagccggc cggctggaca cgccgggtga cgtcgacctt cgacgagacc accgcccttg
      601 gggcgtggcc agggcctgct gctgcccgcg actggttcgc gcgccaggcc ggcgcaagcg
      661 actcgagtgg ccgcggaacg tacgacgcgg gccgaaccgt gaccgagcac gacggcgtcg
      721 tggacgtctt catccactcc gagggcgaca ctcggtacgt ggccgcgctg gtggcggtca
      781 tcggcgatac cgtcggtcag cggatcacga tctgcatgcg ggccgaccgc atccctggat
      841 acaagctggc gtttctcctg tggcccagcc agggggatgg caacgaccgg ggggaaattg
      901 acttcccgga aggaaagttc gagatcggcg ccactgccca cgcgttcatg cactacgacg
      961 gtgccgcggc ccaggacgga tgggataccg gcgtctcgac ggccgattgg cacgtgtaca
     1021 cgatcgagtg gaacccgggc agtgcggcga gccagtcgga cgacgtctgt gcgttctacc
     1081 tggacggcca gctcgtcggg cgatcaacgg gtgcgcccgt acccgacgta ccgatgcatt
     1141 acgtcatgca gatggagacc ttcctcgccg ggcaggccct gcccgcgcca gcctccggcc
     1201 acgtcctgat cgactggatc acgatcgcca ccccgggcta gccggattgc gcggttgcgt
     1261 cggcgaccgg gacctcgtgg tggagacggc agcgggcctc gtacttctcg tcgcccatcc
     1321 cgccgaccat gatcaggggt gagtcggccg gtgccggcag accatcgatc aaccgctgcg
     1381 tgcgggtcgc gggctcgccg cagaccatgc agatggccgt cagcttggtg acctggtcgg
     1441 ccagcgccat gagcatgggc atcggtccga aggggtcacc gcggtagtcc tggtccaacc
     1501 cggtgacgag cacctgcttg ccggcctccg ccagtcgttc gacgacggcg ggcagatcgg
     1561 catcccagaa ctgcccctcc tcgatggcca cgatgtcggc attcccgacc atcgcctcga
     1621 tgtccgccga tccctccacg gacacggccc gatgctgcgc cccagaacgc gacacgaccg
     1681 cctcttcggc ggtcctggtg tcgacccgcg gcttgatgag caccacctgc cgcccggcaa
     1741 ttgcgaagcg gcggagcagg cggagcatct cgtcggtctt gccgcagaac atgcacccgc
     1801 agatgacgtg gacccagccc gatgtgtaga acatcacggt ctgcctgccc ccggctcagt
     1861 cgccgacgac ggcaagtccc tcttcgggca gccccaggtg cgcggccagc gcggtcttca
     1921 tcacgtcggc cggtggctcc tccccggtcc acagctggaa cgccgctgcc tgggcgacga
     1981 ggaacgaccc ctgtccgttg gcgaccgtcc ctccacgggc ggcggcctcg cgcatgagcg
     2041 gcgtctcggc ccggttcagg acgaggtcga ggacgaacag gtcgtgggcc agcagatccg
     2101 ccgggaccgg cgattcctcg gtctcggcac caatgcccga cgcgttgacc agcaggggcg
     2161 ccttggacag ctcggcctcg atgatcgtct cgtgccaggg cagggcgcgc aggtctgtct
     2221 gtcgtgcggc ccggtcgaaa tgtgcgacca ccgcctcggc ccgatggagg tggcggttga
     2281 acaccgcgat ccggccgaga ccggcttcca gcaggaccga tacgaccgcg cgggcacccc
     2341 cgccggcacc cagcacgacg gcctggttcg gccatcgtga cgcgctctcg ggcaggatgg
     2401 actccagccc ggcgcggacg ccggcggcat cggtgttgta gccatggagc cggccgtcgc
     2461 ggttgaccac cgcgttcacc gcgccgctgg cgcgggcctc atcggccagc agaccgatga
     2521 gcgacgcggc cttttcctta tgcggtgagc cgatcatggc gcccataacg tccggctgaa
     2581 ccagctcgcc gagggcgtcg ctcagctggt gggcgcgacg ttcccagcgc tcaatctcga
     2641 cgtcccgacc ggcagccgcc agcgccgccc gctggaccgc ttcggcgggt cctggggcga
     2701 cgggggagcc gatgatgagt aagcgcgtca cgggtcgggt tctcgttcaa aagcagtgtc
     2761 cgggagccgt cagcagggca cgtcgggcca accgggaagg gccggttcca gaggggcaga
     2821 tgctatcatc ggcgccgctt ccggagaggt cgcatagtgg cctagtgcgg cggtttgcta
     2881 aaccgttgaa ggggatttcc tcttccgagg gttcgaatcc ctccctctcc gccagatcgt
     2941 ctcgggtgcc cgtagctcag cggatagagc gttaggttgc ggacctaaag gtcggaggtt
     3001 cgagtcctct cgggcacgcc actcctgtcg
//
LOCUS       DASYGU010000012         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1546046, whole genome shotgun sequence.
ACCESSION   DASYGU010000012 DASYGU010000000
VERSION     DASYGU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546046"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGO32_00350"
     CDS             complement(<1..547)
                     /locus_tag="VGO32_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV7199233.1"
                     /translation="MKIGIDLGTANILVYVQGKGVVLNEPSVVAISEDDNRLVAVGEE
                     AREMIGRTPGNVRAIRPMRDGVIADYLITEAMLHYVINKVARVRIFRPDVMISVPSGV
                     TSVEKRAVRDAALKAGARNAFLIEEPLAAAIGANVPISGPSGNMIIDIGGGTTEVAVI
                     ALGGIVVSHSVRVGGNRFDEAI"
     gene            complement(606..1883)
                     /gene="murA"
                     /locus_tag="VGO32_00355"
     CDS             complement(606..1883)
                     /gene="murA"
                     /locus_tag="VGO32_00355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV7199234.1"
                     /translation="MDRFVIEGGVPLRGEVRVAGAKNAVLKMMAAAILTDEPVVLRNV
                     PQISDVTIMRETMADIGFGWTDVDGHGLELRDRGPEWLFVPLEAAMKMRSSFILLGPL
                     LARHGRVIISNPGGDRIGRRPVDLHVEAMHALGAEIDYRNGYYFARASQLRGARISFP
                     YVTVMGTENALMAACMATGTSVIENAAQEPEVDDLVSMLRAMGARIERTAAHVLEVEG
                     VDSLYGVEHAVLGDRIEAGTFAVAAASSGGEVTVSGFDPSHLGAFNDVLTQMGVEHEP
                     FAANGDSGLRVRGAPAGSYRPVAIETQAYPGLATDLQAPLAVLMTQAAGESTIHETIY
                     SDRLEYTAELVKMGAEIEVADSRHARIVGPTALHGRTVQIPDLRAGATLVVAALGAEG
                     TSTISGVEHVDRGYEAFESKLVGLGARIGRVSD"
     gene            complement(1892..2218)
                     /locus_tag="VGO32_00360"
     CDS             complement(1892..2218)
                     /locus_tag="VGO32_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199235.1"
                     /translation="MLTCAHCGARSGGLPVRIEIVRESGPIGLAGEPDLAGRPDFTRR
                     FAFCSSAHLYEWLRENQYMLCNRQPTQVEEGMQPLLPSRHMGGRLGLCDGHHLGGHEW
                     KEAWPA"
     gene            complement(2232..2420)
                     /locus_tag="VGO32_00365"
     CDS             complement(2232..2420)
                     /locus_tag="VGO32_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199236.1"
                     /translation="MTSEQAKPFRRMQEFRKISRDYPFICPVDFKPHRGSPPGSDPNL
                     DPEFVARHREDDGDHYLE"
     gene            complement(2435..2845)
                     /locus_tag="VGO32_00370"
     CDS             complement(2435..2845)
                     /locus_tag="VGO32_00370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV7199237.1"
                     /translation="MTLRLEVVSPDGRIFTDDVDSVVVPGIEGELGILPQHTSLVTAL
                     GTGELRIRRAGTVQFMLISGGFVEVRPDKVVVMALVAENSEEIDAAAAASARKAAEAD
                     LESAHDPVDVARVRAALQTALMRERIATRRSQRG"
     gene            complement(2870..>3065)
                     /locus_tag="VGO32_00375"
     CDS             complement(2870..>3065)
                     /locus_tag="VGO32_00375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707387.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV7199238.1"
                     /translation="MSQPMFVAAQFTGRDGKYVERKETVRGFREILDGKHDQLPEQAF
                     YMVGSIDEAVAQAEQMASTG"
BASE COUNT          457 a         1072 c         1042 g          494 t
ORIGIN      
        1 tgatcgcttc gtcgaagcgg ttgccgccga cccgaaccga gtgcgagacc acgatcccgc
       61 ccagggcgat gaccgcgacc tcggtcgtgc cgccgccgat gtcgatgatc atgttccccg
      121 acggtccgct gatcgggacg ttggccccga tggccgcggc cagtggctcc tcgatgagga
      181 acgcgttgcg tgcgcccgcc ttcagggcgg cgtctcggac ggcgcgcttc tcgacgctgg
      241 tcacgccgga gggcacgctg atcatgacgt cggggcggaa gatgcggacg cgggcgacct
      301 tgttgatcac gtagtgaagc atggcctcag tgatgaggta gtccgcaatg accccatcgc
      361 gcatcggccg gatagcccga acgttccccg gcgtacggcc gatcatctcc cgggcctcct
      421 cgccgacggc caccagtcga ttgtcgtcct ccgatattgc caccaccgat ggctcgttca
      481 ggacgacgcc cttgccctgg acgtagacaa ggatgttggc ggtgccgagg tcgatcccga
      541 tcttcatgcg cggccgatcg aagcgttggg aatgggcccg gagtatatcg gccggtccgc
      601 ggcgctcagt ccgacacccg cccgatccgg gcgccgaggc ccacgagctt cgactcgaat
      661 gcctcgtagc cgcgatcgac atgctcgacc cctgagatcg tgctcgttcc ttcggcgcca
      721 agggcggcca cgaccagggt cgcgccggcg cggaggtcgg ggatctggac cgtccgcccg
      781 tggagcgcgg tgggtccgac gatgcgcgca tgccgggaat ccgcgacctc gatctcggcc
      841 cccatcttca ccagctcggc ggtgtactcg aggcgatcgg agtagatcgt ctcgtgaatc
      901 gtgctctcac cggcagcctg ggtcatcagc accgccagcg gtgcctggag gtcggtggcc
      961 agcccggggt acgcctgggt ctcgatcgcg accgggcggt agctgcccgc cggcgcgcca
     1021 cggacccgga gtcccgaatc gccgttggcc gcgaacggct cgtgctcgac gcccatctgg
     1081 gtgagcacgt cgttgaatgc gcccagatgg ctcgggtcga acccggagac ggtcacctcg
     1141 ccgccgctcg acgccgcggc cacggcgaag gtgccggcct cgatccgatc gccgagtacg
     1201 gcgtgctcga cgccgtacag cgaatccacc ccctccacct cgaggacgtg cgcggcggtc
     1261 cgctcgatgc gggcgcccat cgcccggagc atggacacca ggtcatcgac ctccggctcc
     1321 tgggccgcgt tctcgatgac cgaggtgccg gtcgccatgc atgcggccat cagcgcgttc
     1381 tcggtgccca tcacggtgac gtaggggaac gagatccgcg ccccacgcag ctggctggcc
     1441 cgcgcgaagt agtagccgtt gcggtagtcg atctcggcgc cgagggcgtg catggcctcg
     1501 acgtggaggt ccaccggacg gcggccgatc cggtcccctc cggggttgct gatgatcacc
     1561 cgcccgtgac gggccagcag cggcccgagc aggatgaacg aggagcgcat cttcatcgcc
     1621 gcctcgagcg gcacgaacag ccattccggg ccccggtcgc gcagctcgag gccgtgcccg
     1681 tcgacatcgg tccagccgaa cccgatgtcg gccatcgtct cgcgcatgat cgtcacgtcg
     1741 ctgatctggg gcacgttgcg gagcacgacc ggctcatcgg tcagaatcgc ggcggccatc
     1801 atcttgagca cggcgttctt ggctcccgca acacggacct cgccgcgaag cgggacgcca
     1861 ccttcgatca cgaagcggtc catcgctcgc cctatgcggg ccacgcctcc ttccactcgt
     1921 gaccgcccag atggtgaccg tcacacaggc ccaaccggcc tcccatgtgt cgtgaaggca
     1981 gcagcggctg catcccctcc tccacctgcg tcggctgccg attgcagagc atgtactggt
     2041 tctcgcgcag ccactcgtac aggtgcgcgc tggagcagaa ggcgaagcgg cgcgtgaagt
     2101 ccggccggcc ggcgaggtct ggctcgcccg ccaggccgat cggcccgctc tcgcggacga
     2161 tctcgatccg gaccggcagc ccccccgagc gggcgccgca gtgcgcgcag gtcaacatgg
     2221 tcgggtgcgc gtcactcgag gtagtggtcg ccatcgtcct cgcggtggcg ggcgacgaac
     2281 tcggggtcga ggttcggatc ggagccgggc ggcgaaccgc gatggggctt gaagtcgacc
     2341 gggcagatga acgggtagtc gcggctgatc ttccggaact cctgcatccg gcggaacggc
     2401 ttcgcctgct cgctcgtcac ccgtgcagcg tagatcagcc ccgctggctc cgtcgggtgg
     2461 cgatccgctc ccgcatgagg gcggtctgaa gggccgcccg aacgcgggcc acgtcgacgg
     2521 ggtcgtgggc cgattccagg tcggcttcgg ccgccttgcg ggccgaggcc gctgcggccg
     2581 cgtcgatctc ctcggagttc tccgccacga gggccatcac gaccaccttg tccggtcgga
     2641 cttcgacgaa gcccccgctg atcagcatga actggaccgt ccccgctcgg cggatgcgga
     2701 gctccccggt acccagcgcg gtgacgagcg aggtgtgctg gggaaggatg ccaagctcgc
     2761 cttcgatccc gggcacgacc accgaatcca cgtcatcggt gaagatccgg ccgtccggcg
     2821 agacgacctc gagtcggagg gtcatcggtc agtcacgggt gtcggctcgt cagcccgtgg
     2881 atgccatctg ctcggcctgg gcaacggcct cgtcgatcga gcccaccatg tagaacgcct
     2941 gctcggggag ctggtcgtgc ttgccgtcga ggatctcgcg gaagccgcgg acagtctcct
     3001 tgcgctcgac gtacttccca tcgcgcccgg tgaactgggc ggcgacgaac atcggctggc
     3061 tcatg
//
LOCUS       DASYGU010000013         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_671794, whole genome shotgun sequence.
ACCESSION   DASYGU010000013 DASYGU010000000
VERSION     DASYGU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671794"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /gene="dnaE"
                     /locus_tag="VGO32_00380"
     CDS             complement(<1..1848)
                     /gene="dnaE"
                     /locus_tag="VGO32_00380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV7199239.1"
                     /translation="MPPAGTGDSFVHLHVHSEFSLLDGMSRLSDLTNRAAELGMPAVG
                     LTDHGALYGAVAFHQAATNAGVKPIIGVETYVAPRGHTEKDPRLDANPSHLILLARNA
                     TGYRNLLALITKAHLDGYYYKPRIDKALLAEHADGLIGTSACLGGEVLKKLAEGDERG
                     ARQAADDYRSILGDGNFFVEVQDHGLPEQRALHPKLVDLARDLNLPLLATNDTHYTLP
                     EQAEAHDLLLCIQTGSNVATPNRLKFETTEFAVKAADEMRRLFNGELPEAMDNTLRVA
                     EMVDLTLEFDRLRLPDFPVPEGDTAASWLRRECERGLAERYGDVTPDIKARLEYELGI
                     IDRMGYSAYFLIVADFTRFAREQGIFTTCRGSAPGSIVTYSLGITPVDPLAYGLPFER
                     FLNPDRVTMPDIDIDFQDSRRGEVIDYVTRKYGADRVAQIITFGTLGAKAAIRDTGRA
                     MGLTYAEADRVAKAIPNELNISLERAIETSPQLREQMAADDRIDKLIGAAKQLEGIAR
                     HASTHAAGIVISREPLTDIMPLQRATDGETTMTQFEMHACEALGLLKFDFLGLINLTI
                     LADAVELIGRHRGVAIDVDNLPLDDTKTFELLSTGETTGIFQLEGSGMRR"
BASE COUNT          267 a          632 c          684 g          331 t
ORIGIN      
        1 ccggcgcatg cccgagccct cgagctggaa gatgccggtc gtctcccccg tcgacaacag
       61 ctcgaatgtc ttcgtgtcgt ccagcggcag gttgtcgacg tcgatcgcga ccccgcggtg
      121 acgaccgatg agctcgaccg catcggccag gatggtgagg ttgatgaggc ccaggaagtc
      181 gaacttcagg agacccagcg cctcgcaggc gtgcatctcg aactgggtca tcgtggtctc
      241 gccgtcggtc gcacgctgga gcggcatgat gtcggtcagc ggctcgcggc tgatcacgat
      301 ccccgccgcg tgagtcgacg cgtggcgggc gatcccctcc agctgcttgg cggccccgat
      361 gagcttgtcg atccggtcgt cggccgccat ctgctcgcgg agctgggggc tggtctcgat
      421 cgcccgctcc aggctgatgt tcagctcgtt ggggatcgcc ttcgccaccc ggtccgcctc
      481 ggcgtaggtc aggcccatcg cccgtccggt gtcgcggatg gccgccttcg ctcccagcgt
      541 gccgaaggtg atgatctggg ccactcgatc ggcgccgtac ttgcgggtga cgtagtcgat
      601 gacctcgccc cgccggctgt cctggaagtc gatgtcgatg tccggcatcg tcacccggtc
      661 ggggttcagg aaccgctcga acgggaggcc gtaggccagc gggtccaccg gcgtgatccc
      721 gagggagtag gtgacgatgc tccccggcgc cgatccccgg caggtcgtga agattccctg
      781 ctcgcgggcg aagcgcgtga agtcggccac gatcaggaag taggccgaat accccatccg
      841 gtcgatgatc ccgagctcgt actcgagcct cgccttgatg tcgggcgtga cgtcgccgta
      901 gcgctcggcc agcccgcgct cgcactcgcg gcgcagccag ctggcggccg tgtcgccctc
      961 gggaaccggg aagtccggca gccggaggcg gtcgaactcg agcgtgaggt caaccatctc
     1021 ggccacgcgc agggtgttgt ccatggcctc ggggagctcg ccgttgaaca ggcggcgcat
     1081 ctcgtcggcc gccttgaccg cgaactcggt ggtctcgaac ttcaggcggt tgggcgtcgc
     1141 cacgttggag ccggtctgga tgcagagcag gaggtcgtgc gcttccgcct gctcgggcag
     1201 ggtgtagtgg gtgtcgttcg tcgcgagcag gggcaggttc aggtcccggg caaggtccac
     1261 gagcttgggg tggagcgccc gctgctcggg caggccgtga tcctggacct ccacgaagaa
     1321 gttgccgtcg cccaggatgg accggtagtc gtcggccgcc tgccgggcgc cgcgctcgtc
     1381 cccctccgcc agcttcttca ggacctcccc gccgaggcag gcggaggtgc cgatcagccc
     1441 atcggcgtgc tcggccagca gcgccttgtc gatccggggc ttgtagtagt agccgtccag
     1501 gtgcgccttg gtgatgagcg cgagcaagtt gcggtagccg gtcgcgttgc gcgcgaggag
     1561 gatgaggtgg ctcgggttgg cgtcgagccg gggatccttc tcggtatggc cgcgcggagc
     1621 gacgtaggtc tcgaccccga tgatcggctt cacgcccgcg ttggtcgcgg cctggtggaa
     1681 agcgacagcc ccgtagaggg ccccgtgatc ggtcaggccc accgccggca tgcccagctc
     1741 cgcggcccga ttggtgagat ccgacagccg ggacatcccg tccagcaggc tgaactccga
     1801 gtgcacgtgg aggtgcacga acgagtcgcc agttcccgcg ggtggcatcc taccgatact
     1861 agtcggcccc gccgcccgtg gttcggggat gccgcgccgg tcaggtcaca ccag
//
LOCUS       DASYGU010000014         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1134701, whole genome shotgun sequence.
ACCESSION   DASYGU010000014 DASYGU010000000
VERSION     DASYGU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134701"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..774)
                     /locus_tag="VGO32_00385"
     CDS             complement(58..774)
                     /locus_tag="VGO32_00385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV7199240.1"
                     /translation="MSHLVVVALVVGSVSVGVLTARGIISIDGMGLQIVAPARGAISE
                     QNAAAQDAPVTIELEPETPVTAEAADGDVMAEIRTLPTPAPEVPVAIILPKPPPVRSG
                     SVGPSLPSAGPTGYVDGDGSLTWPVPGGYVSQYFWSGHLGLDVAHAAGGAVVAADTGT
                     VTQAGWLNNGGGMVITIAHADGRTTQYNHLGTILVSAGQGVVRGQQIAAVGCSGWCTG
                     PHVHFVVIVNGVIVNPLRFL"
     gene            complement(968..2347)
                     /locus_tag="VGO32_00390"
     CDS             complement(968..2347)
                     /locus_tag="VGO32_00390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV7199241.1"
                     /translation="MRLRRELRRIRPLRPGLAIGLILVLVLAGCVGSASPSVSAQPST
                     SINPSAPASAGPGEVIPAPGSSSRIFAPNPRAIVVAIDPGHGGCLDWGVPNPYDNTVE
                     RSEKTLTLGISLALREHLEAEGVTVVMTRDTDVALAGDDYPPLGCEGDPFRDVNGDGE
                     AGFGPEVSESTRTRDELAAHIDLVNLARADVLLSIHINSFTENGVVIEVAGTETYWTD
                     ETAWGVPHSERLASAIQAAVVAAIGAIAPYERQDRGIDAVNYYVIAPATTTGDPSEPR
                     RGSLMPGVLAEVGSMSLAAEADLLASAEGQEAIAVAITDALVTWFAGRELGVRIDLAA
                     PGGAAGVAPAVVPGTGPPYWAPSVPDPAALAIRLTNTGTAPWPAGTMLVGGWGSATEP
                     YLARPPELVPLATAVPPLAPGESVDLEASMPPIPAGEGAIGWVTIRVGDVVLSESGIP
                     ALQFVPGRP"
     gene            complement(2344..3627)
                     /gene="rho"
                     /locus_tag="VGO32_00395"
     CDS             complement(2344..3627)
                     /gene="rho"
                     /locus_tag="VGO32_00395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV7199242.1"
                     /translation="MTLGELQEKSIEELRDLATDYGIEGDSLEHSDLVLKLLDAVPLA
                     QTSQREEMVVGEVVGVLEIVDEGFAFLRREGVMPSPDDIYVSSSIVRRFGLRTGDRVV
                     GQSRPPKDQERYWGLARVDTVNGIDPELARRRPNFDSLVPVFPDEMFDLEIDQKNLTQ
                     RLINLIAPVGKGQRGLILSPAKAGKTTVLKNIAHGITENHPDALLMVALIGERPEEVT
                     DMQRSVKGEIFASTFDEPTENHTRVAEMTLEIAKRQVETGRDVVILLDSITRLARAYN
                     LALPASGKTLSGGVDPAALYPPKRFFGAARNIEGGGSLTVIATCLVDTGSRMDDVIYE
                     EFKGTGNMELVLDRKLSEKRIFPALDVLRSGTRREELLLDDNTLKMIWTMRRMLSALG
                     PNEGIELLMQRLGKTKNNSEFLVQLSKSVDASEGR"
BASE COUNT          572 a         1261 c         1327 g          557 t
ORIGIN      
        1 cacgtggatt cgaacgcggt ccgcgaacat cgtggcccga ccgtgaggcc ggagcggcta
       61 caggaagcgc agcgggttga cgatgacgcc gttgacgatg accacgaagt ggacgtgagg
      121 accggtgcac cacccggagc acccgaccgc cgcgatctgc tggccccgaa ccacgccctg
      181 ccctgcgctg accaagatcg tgccgaggtg gttgtactgg gtcgtccgcc cgtccgcatg
      241 ggcgatggtg atcaccattc cgccgccgtt gttcagccag ccggcctgcg tgacggtccc
      301 cgtgtccgcg gccaccacgg cgccgccggc ggcgtgagcg acgtcaaggc caaggtgccc
      361 gctccagaag tattgggaga cgtagccgcc cggcaccggc caggtgaggg acccgtcgcc
      421 gtcgacgtag ccggttgggc ccgccgacgg gagggaaggt cccacggagc cggagcggac
      481 cggcggcggc ttgggcagga tgatcgcgac cgggacctcg ggtgccgggg tcggcagggt
      541 gcggatctcg gccatgacgt cgccgtcggc ggcctcggcc gtgaccggag tctcgggttc
      601 gagctcgatc gtgaccgggg cgtcctgggc ggccgcgttc tgctcggaga tggcgcctcg
      661 tgccggggcg acgatctgga ggcccatccc gtcgatgctg atgatgcccc gtgcggtcaa
      721 cacgccgacg ctgacgctgc caaccaccaa cgcgacgacc acgaggtggg acaaggcccg
      781 cgcaacggcg gtatagctcc gacgacgacg gcgcaccggc cgggacgagt caaccaatag
      841 cgacggctcc tgggcgtgtt cttctcaaaa cagcacgacg ccgatgtgcg atcccgcaga
      901 atcgcctggg cacgggcgag gcgtcgtgcc ccgcgatcct agcaagggca tcaaaggcag
      961 gtcaacctta cggacgtccg ggcacgaact gcaaggccgg aatgccggat tcggacagga
     1021 cgacgtctcc gacccgaatc gtgacccatc cgatggcgcc ttcgccggcc gggatgggcg
     1081 gcatggacgc ctccaggtcg accgattccc ccggggccag gggcggcacc gcggtggcca
     1141 gcgggaccaa ctccggcggt cgagcgaggt acggctcggt cgcgctcccc cacccaccca
     1201 cgagcatcgt cccggccggc catggagccg tccccgtgtt cgtgaggcgg atggcgagcg
     1261 ccgccggatc ggggacggag ggtgcccagt acggcgggcc ggtgcccggg acgacggccg
     1321 gggccacgcc ggcggcgccg cccggcgccg ccagatcgat gcgaacccca agctcgcgac
     1381 cggcgaacca ggtgaccagc gcgtccgtga tcgcgacggc gatcgcctcc tgcccctcgg
     1441 ccgaggccag cagatcggcc tccgcggcca gcgacatcga gcccacctcg gccaggacgc
     1501 cgggcatgag gctgccgcgc cgcggctcgc tcgggtcgcc ggtcgtggtg gccggggcga
     1561 tgacgtagta gttcaccgcg tcaatgccgc ggtcctggcg ctcgtagggc gcgatcgccc
     1621 cgatcgcggc caccaccgcg gcctggatcg cgctggccag tcgctcggag tgcggcacgc
     1681 cccacgccgt ctcatcggtc cagtaggtct ccgtgccggc gacctcgatc acgaccccgt
     1741 tctcggtgaa cgagttgatg tggatgctga gcaggacatc ggcccgggcc aggttgacca
     1801 ggtcgatgtg ggccgcgagc tcgtcgcggg tgcgggtgga ctccgacacc tcgggcccga
     1861 acccggcctc cccatcgccg ttcacatcgc ggaacgggtc gccctcgcat ccgagcggcg
     1921 gatagtcgtc cccggccagt gcgacgtcgg tgtcgcgggt catgaccacg gtcacgccct
     1981 ccgcctcgag gtgctcgcga agggccagcg agatgcccag ggtcagcgtc ttctcggacc
     2041 gctcgacggt gttgtcgtac gggttgggaa cgccccagtc caggcagccg ccgtgccccg
     2101 gatcgatggc caccacgatc gcacgaggat tgggggcaaa gatccgggag ctcgagccgg
     2161 gggccgggat gacctcaccc gggccggcgc tggccggcgc ggatgggttg atgctggtcg
     2221 acggctgggc cgagaccgat gggctggccg aaccgacgca gccggcaagg acgaggacga
     2281 ggatcaggcc gatggccaga ccgggacgga gtggacggat ccgccgcagc tcgcggcgca
     2341 gcctcatcgg ccttcggagg cgtccacgct cttcgacagc tgaacgagga actcggagtt
     2401 gttcttcgtc ttgcccagtc gctgcatgag cagctcgatc ccctcgttcg ggccgagcgc
     2461 gctcagcatc cggcgcatgg tccagatcat cttcagggtg ttgtcgtcga ggagcagctc
     2521 ctcgcgacgg gtgccggagc gaagcacgtc gagggccggg aagatccgct tctcgctgag
     2581 cttccggtcg aggacgagct ccatgttgcc cgtgcccttg aactcctcgt agatgacgtc
     2641 gtccattcgc gaaccggtat ccaccaggca cgtcgcaatg acggtgaggc tcccccctcc
     2701 ctcgatattc cgcgccgcgc caaagaagcg cttgggcgga tacagggccg cgggatccac
     2761 gccgccggac agggtcttgc ccgatgcggg gagggccagg ttgtacgcgc gcgccaaccg
     2821 ggtaatggag tccagcagga tcaccacgtc acggccggtc tcgacctggc gcttggcgat
     2881 ctcgagggtc atctccgcca cgcgggtgtg gttctcggtc ggctcgtcga aggtggaggc
     2941 gaagatctcg cctttcaccg accgctgcat gtcggtgacc tcctccggtc gctcgccgat
     3001 gagggcgacc atgaggagcg cgtcggggtg gttctcggtg atcccgtggg cgatgttctt
     3061 caggacggtc gtcttccccg ccttggccgg ggacaggatc agcccgcgct ggcctttgcc
     3121 gaccggcgcg atcaggttga tgagccgctg ggtgaggttc ttctggtcga tctcgaggtc
     3181 gaacatctcg tcggggaaga ccgggaccag cgaatcgaag ttcggacggc ggcgtgccag
     3241 ctcgggatcg atcccgttca cggtgtcgac ccgggccagg ccccagtacc gttcttggtc
     3301 cttcgggggt cggctctggc cgacgacccg gtcgccggtg cgcaggccaa atcggcggac
     3361 gatgctggac gacacgtaga tgtcatccgg cgagggcatg acaccttcgc ggcgaaggaa
     3421 ggcgaagccc tcgtccacga tctcgagaac gccgaccacc tcgccgacga ccatctcctc
     3481 ccgctgggac gtctgcgcca ggggcaccgc gtcgagcagc ttcaggacga ggtcggagtg
     3541 ctcgaggctg tcgccctcga tcccgtagtc ggtcgcgagg tcacgcagct cctcgatgga
     3601 cttctcctgg agctcgccca gggtcagggc cggctcatcg agcgcctgcg ggtacggacc
     3661 ggcatagacc gatgaatacc cgcccgcgag gtcgcggcgg cgctggaagt tgccgcg
//
LOCUS       DASYGU010000015         3779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_569033, whole genome shotgun sequence.
ACCESSION   DASYGU010000015 DASYGU010000000
VERSION     DASYGU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569033"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..973)
                     /locus_tag="VGO32_00400"
     CDS             complement(35..973)
                     /locus_tag="VGO32_00400"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEV7199243.1"
                     /translation="MKTSRSALLVLLTALVLILAACQQASTGAKPEEVPAGVGGEGRD
                     VPGGAPTVVDEDGNFSGDPGAPAEADRSIIRTGEITVEVDNVPETVGEVRALAVELDG
                     FISNSFQGEFEDSATLTMRIPADRFDDAIAAIHDLDGEVKAEATNEEDVTAVVIDIEA
                     RLENLRAAEEEYRNLLDRAETVEDILAIQNQLFAVRGEIESYQAQLEYYTDQVDLATL
                     TVSIIPVPEPVQAATEDFDPTAIAKEAVASLVSFGQWLVSAGIWLLIVGLPALIVFGI
                     VAFVAWKIFRRVRPTKGSQAPQTPEAPAAGAGTPVT"
     gene            1061..2278
                     /locus_tag="VGO32_00405"
     CDS             1061..2278
                     /locus_tag="VGO32_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199244.1"
                     /translation="MNRPNRTLLALVAAVASASTAYIAVWVVSSLVVLEITGSALAAG
                     VPSALAGLATAFGAVGVANLGIRWNRPRAVATGFVAALIGCGLAVASILLASLPWLFA
                     ASLAFGLGNGALQQTRFAAADTVAADRRAWALGIAVWGSTVGAVFGPNLVGVAEALAL
                     PIGADPLVLSLGIIGLLFGTAVLFALLAGRWTARLPDEPGGERQAGWAGRRIGSLLRD
                     PHRRSALAALLGAQVAMVLIMTMTPLHIHAGGGDLVFVGLVISAHTLGMFALAPLSAR
                     LVSRQGATPVALAGMVVLAVAAVLSAVAPPHDGPLLALALFLLGYGWNLSFVAGSAML
                     VEGAPSAAQTQRQGASDFLVFLAGATASIMSGALLAVVGFAAMSLLGGAFLVVPAAII
                     LRERTRGPEPAPA"
     gene            2326..>3779
                     /locus_tag="VGO32_00410"
     CDS             2326..>3779
                     /locus_tag="VGO32_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV7199245.1"
                     /translation="MVGPDAYCELHVHSNFSFLDGASHPEDLVARAARIGMPALAITD
                     HAGLYAAVRLWKAADATRTEDARGASLSPVVPILGVELAIPRDERELRLARRGRRMAL
                     PLRGTKASRGWPGEHHAGPAPGDHLVLLACDPAGYAALSGIVSAGHLAGEKAFPVFEA
                     AALEAALDEAKGHLIGLSGCRNGEIPRRLLAGERAMARMAADRWAGHFPDGDFCVELS
                     HHLLPDDDWLVAELASLADEAGLPTVVTNEVHYAEAAGHRLQDVLVAIRHGASLDDTR
                     ELRLQNAEYRLKTGPQLALIGAGLPDPRARRAWHDGMAQAAAIGQACRLDLGFERYRF
                     PGFTVPDGETPFSYLYQLAHDGLRRRYRPITKQALEQLTHELGVIDRTNLSEFFLIVW
                     DLMEFARRNKIIGQGRGSAGDSIVAYCLGITKVDPIEHKLLFERFINEARALPDIDID
                     FDVNRREEVIQYLYRRYGVDHAAMVCNVITYRARS"
BASE COUNT          499 a         1341 c         1311 g          628 t
ORIGIN      
        1 ggtggacgag ccgggcgcta tcggtcccgc cggatcaggt gaccggcgtt cccgctcccg
       61 cggctggcgc ttccggggtc tgtggcgcct gcgaaccctt cgtcgggcgg acccggcgga
      121 agatcttcca ggccacgaac gccacgatcc cgaacacgat cagggccggg aggcccacga
      181 tcaggagcca gatgccggcg ctgaccagcc actgaccaaa gctcacgagc gaggcgaccg
      241 cttccttggc gatggccgtc gggtcgaagt cctcggtcgc cgcctgaacg ggttcaggga
      301 ccgggatgat ggagaccgtc agcgtcgcca ggtcgacctg gtcggtgtag tactcgagct
      361 gtgcctggta cgactcgatc tcgccgcgaa ccgcgaagag ctggttctgg atggcgagga
      421 tgtcctcgac cgtctccgcc cggtcgagga ggttgcggta ctcctcctcg gcggcgcgaa
      481 gattttcgag ccgcgcctcg atgtcgatga caacggcggt aacgtcttcc tcgttggtgg
      541 cctccgcctt cacttcgccg tcaaggtcgt ggattgcggc aatggcgtcg tcgaagcggt
      601 cggccgggat ccgcatggtg agggtcgcgg agtcctcgaa ctcgccctgg aacgagttgc
      661 tgatgaagcc atccagctcg acggcgaggg ctcggacttc gcccacggtc tcggggacgt
      721 tgtcgacctc gaccgtgatc tcgccggtcc ggatgatgga ccgatcggcc tcggccggcg
      781 cgccggggtc gccggagaag ttgccgtcct cgtcgacgac cgtcggtgcg ccgccgggga
      841 cgtcacgacc ctcgccgccg actcccgcag gcacttcttc gggcttggca cccgttgagg
      901 cctgctggca ggcggccagg atgaggacca gtgcggtgag caggaccagg agcgcggatc
      961 gtgaggtctt cacaggggtc tccagggttt ggttatcagc ccccagacgc cgcaggtcga
     1021 aacgcggttc ccaaccgtgg tcggcccata tgatcccgag gtgaatcggc ccaaccgcac
     1081 gttgctcgcg ctcgtggccg ccgtggcctc ggccagcacg gcctatatcg ctgtctgggt
     1141 cgtgagctcc ctggtcgtcc tcgagatcac cgggtccgcg ctcgcggccg gcgtacccag
     1201 cgcccttgcc gggttggcga ccgccttcgg cgccgtcggc gtcgcgaacc tggggatccg
     1261 ctggaaccgg ccgcgggccg tggccaccgg cttcgtggcg gcgctcatcg gatgcggttt
     1321 ggccgttgcc tccatcctcc tcgcatccct cccttggctg ttcgcggcca gtctcgcgtt
     1381 cgggctcggc aacggggcgc tccaacaaac ccgattcgcg gccgccgata cggtcgccgc
     1441 cgaccgccgg gcgtgggcac tgggcatcgc ggtgtgggga tcgacggtgg gcgcggtgtt
     1501 cgggccgaac ctggtcgggg tggccgaggc gctcgcgctg ccgatcggcg ccgacccgct
     1561 cgtgctgtcg ctcgggatca ttggcctcct gttcgggacc gccgtcctgt tcgccctcct
     1621 cgccgggcgc tggacggctc gcctaccgga tgaaccaggc ggggagcggc aagccggctg
     1681 ggcaggccgc cggatcggct ccctgttgcg ggatccgcac aggcggtcgg cgctcgcggc
     1741 gctgcttggc gctcaggtcg cgatggtgct gatcatgacc atgacgccgc tccacattca
     1801 cgccggtggt ggggacctcg tgttcgttgg actcgtgatc agcgcccaca cgctcggcat
     1861 gttcgcgctg gccccgctct cggcccgact ggtcagccgg cagggggcca cgcccgtcgc
     1921 gctcgccggg atggtcgttc tcgccgtcgc cgcggtcctg tcggctgtgg ccccgcctca
     1981 cgacgggccc ctcctcgcgc ttgccctctt cctcctcggc tatggctgga acctgagctt
     2041 cgtggccggc agcgcgatgc tggtcgaggg cgcaccgtca gcggcccaga cgcagcgcca
     2101 gggagcgagc gacttcctgg tctttctcgc cggggcgacg gccagcatca tgtccggcgc
     2161 gctgctggcg gtcgtcggtt ttgcggcgat gtcgctcctg ggcggggcgt tcctcgtcgt
     2221 cccggccgcg atcatccttc gcgagcggac gcgcggtccg gagcccgccc ccgcttgagc
     2281 tagggtctgg acatatagaa caatcgttct atactcgacc gtccgatggt tggaccggac
     2341 gcgtactgcg agctccacgt tcacagcaac ttctcgttcc tcgacggggc aagccacccg
     2401 gaggatctcg tggcccgggc ggcccggatc gggatgccgg cgctcgccat caccgaccac
     2461 gcggggctgt atgccgcggt ccggctgtgg aaggcagccg acgcgacccg gaccgaagac
     2521 gcccgcggcg cgagcctctc cccggtggtg ccgatccttg gcgtggagct cgccattccc
     2581 cgcgatgagc gcgagctccg cctcgcgcgg cgcgggcggc gcatggcgct cccactccgc
     2641 ggcacgaagg ccagccgcgg ctggccgggg gagcatcacg ccggtcccgc ccccggcgac
     2701 cacctcgtcc tgctggcttg cgacccggcc gggtacgccg ccctgtcggg catcgtgtcc
     2761 gccggccacc tggccggcga gaaggcgttc cccgtgttcg aggcggccgc gctggaggcg
     2821 gcgctggacg aggcgaaggg ccatctcatc gggctgagcg gctgccggaa cggagagatc
     2881 ccgcggcgac tgcttgccgg ggagcgggcc atggcccgga tggcggcgga ccggtgggcc
     2941 ggccacttcc ccgacgggga cttttgcgtg gagctgtccc accacctgct ccccgacgac
     3001 gactggctcg tggcggagct cgcctccctc gccgacgaag cgggcctgcc caccgtcgtc
     3061 accaacgagg tccactacgc cgaggcggcc ggccatcggc tccaggacgt gctggtcgcc
     3121 atccgccacg gcgcctcgct cgacgacacg cgcgagctcc gcctccagaa cgccgaatat
     3181 cggctcaaga ccgggcccca gctcgcgctc atcggggccg gactacccga tcctcgcgcc
     3241 cggcgtgcct ggcacgacgg gatggcgcag gcggccgcca tcggtcaggc ctgcaggctg
     3301 gacctgggct tcgagcgcta tcggttcccc ggcttcaccg ttccggacgg cgagacgccc
     3361 ttctcgtacc tgtaccagct tgcccatgac ggcttacggc gccggtatcg gcccattacc
     3421 aagcaggcgc tcgagcagct gacccacgag ctgggcgtca tcgaccgcac gaacctgtcc
     3481 gagttcttcc tgatcgtgtg ggacctcatg gagttcgcgc ggcggaacaa gatcatcggc
     3541 caggggcggg ggagtgctgg cgactcgatc gtggcctact gcctgggcat caccaaggtc
     3601 gatccgatcg agcacaagct cctgttcgag cgcttcatca acgaggcgcg ggccctgccc
     3661 gacatcgaca tcgacttcga cgtcaaccgg cgcgaggagg tcatccagta cctctaccgc
     3721 cgatacggcg tcgaccacgc ggccatggtc tgcaacgtca tcacctaccg cgcccggtc
//
LOCUS       DASYGU010000016         9757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1546201, whole genome shotgun sequence.
ACCESSION   DASYGU010000016 DASYGU010000000
VERSION     DASYGU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546201"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VGO32_00415"
     CDS             <1..1295
                     /locus_tag="VGO32_00415"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7199246.1"
                     /translation="YECPNVRIRGRAMATNTPPNGAFRGFGAPQTEFAAETQVNRIAE
                     RLRISPLALRRRWVYREGGVTPTGQVLRESVAGLEVLEAAAQASEYEGVAGHTKKARE
                     QRREDARTARGIGLALAWHGAGFTGSGEVHLASVASLELTNDGEIRILTASTEMGQGT
                     KTIFPQLVGETLGIDQADVEIAPQDTSIVPDSGPTVASRTAMVVGGLLIKAAEKMRTE
                     VEQAVGGPFADTYRSFARVHGPLRIDEQFTPYPGVEFDDTTYTGDAYPCFGWAAAVVE
                     VDVDLDTGEVAVTDVVSADEIGRVIHQVLAEGQVEGGSLQAVGYATIEELKLVDGRYQ
                     NDRLATYLIPTAMDAPRITSLLLEHPYSGAPHGAKGVGELPMDVVAPAVVAAIHDATG
                     FWITDLPASSERVLAALTGSAPPAPPGISAEQPPDRAA"
     gene            1300..1794
                     /locus_tag="VGO32_00420"
     CDS             1300..1794
                     /locus_tag="VGO32_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV7199247.1"
                     /translation="MTYRLTVNGVPSEVEAPGMRRLLDVLREDLGLTGTKEGCGEGEC
                     GACSVLVDGMVVDSCLVPLCQVNGSTVDTVEGLGAPGALDVLQAAFLETGGAQCGICT
                     PGMLMAGEAFLASGAEPTEDAVREAIAGNLCRCTGYTKIIDAIVLAAQRRQDGPLPPP
                     DQPA"
     gene            1798..2655
                     /locus_tag="VGO32_00425"
     CDS             1798..2655
                     /locus_tag="VGO32_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV7199248.1"
                     /translation="MPAEPPVESPATLADAYRLLADGGSWRPVAGGTDLLVQITGELG
                     PPPDRVLDIWALDELRGIALDGDELVIGALTTYTDLRQSALVGELVPALAAASATIGA
                     AQIQNRGTVGGNLVNASPAGDTLPIWLATDTQIVLGSAGGERSVAADEFFTGYRQTAR
                     REDELVLRVRVPLLPRRHVRFRKVGTRRAQAISKVVLSLSWLTAEDGAWHDTRLALGS
                     VAATPIRARATEAVLDGRHPVRATAAAAVAALEAEIKPIDDVRSTADYRKLVTGRVLR
                     RLIRQEGGW"
     gene            2661..2957
                     /locus_tag="VGO32_00430"
     CDS             2661..2957
                     /locus_tag="VGO32_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199249.1"
                     /translation="MHEPKGSRLIGAVLLVVIIVVVFIGLWAFLSMAMVQGGFPSGSV
                     VFTIGAIFLGAVAIATAVWLIRLVQARNEAGGVADQIPVPPPDDDTTAAPDLDG"
     gene            2982..5750
                     /locus_tag="VGO32_00435"
     CDS             2982..5750
                     /locus_tag="VGO32_00435"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7199250.1"
                     /translation="MIEPRSGTVTLLFADIAGSTRMLAELGDGYGAVLREYRRLVEEA
                     AMAEHGSLLDTAGDGVFYSFPSARGALSAALAAQRSLRDHPWPADTRVEARIGIHTGE
                     PLTSDESLVGMDVHRAARICAAGHGGQILLSMTTHDLLRGTALPDATLRDLGEHRLKD
                     LPHPERLYQATAPDLPSEFPALRSLDNWPNNLPRQLSSFVGRAAALDDVRARLAETFL
                     LTLVGPAGVGKTRLGCEVALQALDQFPDGAWVVELGALTDGALLAETVASTLRIKEQP
                     GTPLLQTLADHLEARRLLLLFDDCEHLIDATAEVIGPLLQAGGDSRILVTSREPLAIP
                     GESLYPVATLELPAPEGATVDDAADTEAVRLFVDRAVAVQPTFVLTAENTAAVIQICH
                     RLDGIPLAIELAAARVKALPPAQIASRLDDRFRLLTGGSRMALPRHRTLKAALDWSFD
                     LLTDAERALLVRLSVFAGGFGLDAIEEVCGGDVVDTDDVLDLLTRLIDRSLVVAEISA
                     GEARYRLLDSVQQYAAELLAADDPEGAINVRHQAFVVELVERLGAAMLAGGPPGADAP
                     FRQLVAERENVRAALQRADDDPSAAAQELRLAANLGRYWEIGGLLIEGRSWLTRALAR
                     TDGEVSDLRANALTSLGTLAIQQGDFAAATTAHEKALATQRQLNNPNAVAYAASNLAN
                     SWAERGELDRARELYEEALAILAALSDPRASALAKLNLADVADRQGHQDEARRLVDES
                     VETFRAERDPIGIALSQGRGATFSLRHGDRADARRRHDEALEIYRMFGDGRGVARTLM
                     FIGDIVALEGDMDEAERLYREAIDQRWALGDRGGLATACDRLARASALADPERSARLI
                     GFADAQRDAIGASLPSQDATERDELIGSLDGRLGTAEFGGLRHAGRRLPLVTVLLPSP
                     APAGGSGA"
     gene            complement(5747..5998)
                     /locus_tag="VGO32_00440"
     CDS             complement(5747..5998)
                     /locus_tag="VGO32_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEV7199251.1"
                     /translation="MPLYLDTHNHVAGLTGDAVAQAHARDLEVQGKYNVSYLRYWYDE
                     ATGKVYCLVEAPSAEAAEEVHREAHGLLADLIQEVTEGA"
     gene            6074..6679
                     /locus_tag="VGO32_00445"
     CDS             6074..6679
                     /locus_tag="VGO32_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV7199252.1"
                     /translation="MRRPTRDEVHVFPTTADFRAWLEANHATADELWVGYYRKSTGKT
                     AMTYPEAVEEALCFGWIDGLTFRIDDEVHANRYTRRRKGSSWSAPNIAKVAELAAAGR
                     MHPAGARAFEERDRKKDAPYMRDHPLRQQLPAELEARIAANPAAWAYWQAQRPGFRRG
                     AAFWIQSAKQETTRERRLASLIEESAAGRVPNALRRPGEDQ"
     gene            6745..8298
                     /gene="aceB"
                     /locus_tag="VGO32_00450"
     CDS             6745..8298
                     /gene="aceB"
                     /locus_tag="VGO32_00450"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEV7199253.1"
                     /translation="MTRTGGAHHEEVLTAHALAFVAGLHRSFAVRRQHLLTDRAARQA
                     RFDAGERPDFLPNTAHVRGDLGWRVAPAPADLDDRRVEITGPVEPKMMINALNSGARV
                     FMADFEDALSPTWENVVTGQWAVREAVRRRLTFQTDEKAYALNPEIATLVIRPRGWHL
                     DEANVLVDGAPISASLFDFGLMFFHNAREQLVRGSGPYFYLPKLESHLEAALWNDVFL
                     AAQAALDIPRGSIRATVLIETILAAFEMEEILYELREHAAGLNAGRWDYIFSIIKKFR
                     SRPEMVLPDRAQVTMAVPFMRAYQQLLVQTCHRRGAHAIGGMSAFIPNRREPEVTEKA
                     LDQVRADKERESGDGSDGTWVAHPDLVPLVREIFDGVLGERPNQKDHLPEAIVTSDEL
                     LNVEVAGGRVTEVGVRTNITVALQYLASWLAGNGAAAINNLMEDAATAEISRSQLWQW
                     RVRGTPLEDGRPMTAEVYASIRDEELSRLEADMPGFRWTDAASLLDDLVLSDDFAEFL
                     TTAAYPRLG"
     gene            complement(8221..9501)
                     /locus_tag="VGO32_00455"
     CDS             complement(8221..9501)
                     /locus_tag="VGO32_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008414128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEV7199254.1"
                     /translation="MNEPEIESGQRVTPLELFFDLVFVFAITQVTGFLADDPTAGGLF
                     RGLLLLAALWWAWAAYAWLTNTLNPEEGVVRLAVFAAIGAMLIVALATPEAFGTEGVV
                     FGIAYFAVRALHLVLYAIAGKGDPELLGAVLRITPTAIAGPLMLVVAGFLDGAAQIAL
                     WVAALAIDYLGALVGRARGWRVSPEHFVERHGLVVIIALGESVVAIGIGAAGQPLDAG
                     TIIAALLGITVIATLWWSYFDWVSFMVLARLEEASGTERAALARDLYSYLHLPMVAGI
                     VLFAFGLKTTIAHVSEPLLAIPAVGLCGGLALYFLAHVATRVRIGGGLGRGRPIATIV
                     LIGLTPVATVVPALAALALVTAVCVALIAYEALRYRYARTWIRSRRGHFTLDEARNLG
                     RMGGGAVVPSAEPRIGSRGEELGEVVGQDEIVEK"
     gene            complement(9501..>9757)
                     /locus_tag="VGO32_00460"
     CDS             complement(9501..>9757)
                     /locus_tag="VGO32_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEV7199255.1"
                     /translation="IEVGRDADLVLFDPAADRVIDQDDLHHTSDFTPFEGMAVPGSVR
                     SVLVRGRAVVEAGEFLGERRRGRFLERSLPGGAGGSMRGG"
BASE COUNT         1519 a         3441 c         3391 g         1406 t
ORIGIN      
        1 cgtatgaatg cccgaacgtc cggatccgtg gtcgggcgat ggccaccaac accccgccca
       61 acggggcctt ccgcggattc ggtgccccgc agaccgagtt cgcggccgag acccaggtca
      121 accgcattgc cgagcgactc aggatctccc cactcgccct ccgtcggcgg tgggtgtacc
      181 gcgagggtgg cgtcacgccc accggccagg tgcttcgcga gagcgtggcc gggctggagg
      241 tcctcgaggc agccgcccag gcgagcgagt acgaaggcgt ggcaggacat accaaaaagg
      301 cgcgcgagca acggcgcgaa gacgcccgga ccgcccgcgg catcggtctg gcgctcgcct
      361 ggcacggggc cgggttcacc ggctccggag aggtccacct cgccagcgtg gcttcgctcg
      421 agctcacgaa cgacggtgag atccggatcc tcaccgcctc gaccgagatg ggccagggca
      481 cgaagacgat cttcccgcag ctggtcggcg agacgctcgg catcgaccag gcggacgtcg
      541 agatcgcgcc ccaggacacc tctatcgtcc ccgactccgg gccgacggtc gcctcgcgga
      601 cggccatggt cgtgggcggc ctcctcataa aagctgccga gaagatgagg accgaggtcg
      661 agcaggcggt cggtggcccg ttcgccgaca cctatcggtc cttcgcccgc gtacatggcc
      721 cgcttcggat cgacgagcag ttcacgccat atcccggtgt cgagttcgac gacacgacct
      781 acaccggcga cgcgtaccca tgctttggct gggccgcggc ggtggtcgag gtcgacgtgg
      841 acctcgatac cggcgaggtc gcggtgaccg atgtcgtgag cgcggacgag atcggccggg
      901 tcatccacca ggtcctggcc gagggccagg tcgagggcgg ctcgctccag gccgtgggct
      961 acgcgacgat cgaagagctc aagctggtgg acggtcgcta ccagaacgac cggctggcga
     1021 cctacctgat tccgaccgcg atggacgcgc cgcggatcac ctccctcctc ctcgagcatc
     1081 cgtacagcgg tgcgccgcat ggcgcgaagg gtgtgggcga gctgccgatg gacgtcgtcg
     1141 cgccggcggt ggtcgccgcc atccacgacg cgacgggctt ctggatcacg gacctgccgg
     1201 catcgtccga gcgggtgctg gccgccctga ccggctcggc accgccggcg ccgccgggaa
     1261 tcagcgccga gcagcccccg gaccgggcgg cgtaatggca tgacctaccg gctgaccgtc
     1321 aacggcgtcc cgtccgaggt cgaggccccg ggcatgcggc gcctgctcga cgtcctccgc
     1381 gaggatctcg gcctgaccgg caccaaggag ggctgcggcg aaggcgagtg cggcgcgtgc
     1441 tcggtcctgg tcgacggcat ggtcgtggac tcctgcctcg tgccgctgtg ccaggtcaac
     1501 gggtcaaccg tcgacacggt cgagggcttg ggcgcgcccg gtgctctcga cgtcctgcag
     1561 gccgcattcc tcgagaccgg cggcgcgcag tgcgggatct gcacgccggg aatgctgatg
     1621 gccggtgaag ccttcctggc gtcgggcgcg gagcccaccg aggacgccgt ccgcgaggcg
     1681 atcgccggca acctgtgtcg gtgtaccggc tacaccaaga tcatcgacgc gatcgtgctg
     1741 gctgcgcagc ggcgccagga cgggccgctc cctccgccgg atcagcccgc ctgaccgatg
     1801 cccgccgagc cgcccgtcga gagcccggcg accctggccg atgcgtatcg gctcctggct
     1861 gatggcggga gctggcgccc ggtggcaggt gggaccgacc tcctggtcca gatcaccggc
     1921 gagctcgggc caccaccgga ccgcgttctg gacatctggg cgctggacga gctccgcggg
     1981 atcgcccttg acggtgacga gctggtcatc ggcgccctca ccacctacac cgacctgcgc
     2041 cagtccgcgc tcgtgggcga gctcgtgccc gcgctcgcgg cggcatccgc gaccatcggc
     2101 gccgcacaga tccagaaccg gggcacagtc ggcgggaatc tcgtgaacgc gtccccggcg
     2161 ggcgatacgc tccccatctg gctcgcgacc gatacccaga tcgtgctggg cagcgccggc
     2221 ggggaacgat cggtggcggc ggacgagttc ttcaccggct accggcagac ggcgcgacgg
     2281 gaggacgagc tcgtgctccg ggtgcgcgtc ccactgctcc ctcgccgcca tgtccgattc
     2341 cggaaggtgg ggacccgtcg cgcgcaggcg atcagcaagg tcgttctgtc cctctcgtgg
     2401 ctcactgccg aagacggggc gtggcacgac acgcgcctcg cccttggatc cgtcgcggca
     2461 acccccatcc gggcacgggc cacggaagcc gtcctcgacg gacggcatcc ggtcagggcg
     2521 accgccgcgg ctgccgtcgc ggccctcgag gcggagatca agccgatcga cgacgttcgg
     2581 tcgacggcgg actaccgaaa gctggtcacg ggccgcgtgc tccgtcgcct catccggcaa
     2641 gaaggaggtt ggtgaccctc atgcatgagc cgaagggatc tcgactcatc ggcgccgtgc
     2701 tcctggtggt gatcatcgtg gtggtgttca tcggcctgtg ggcatttctg tccatggcca
     2761 tggtccaggg tggttttccg agcggttcgg tcgtgttcac gattggcgcg atcttcctcg
     2821 gcgcggtggc cattgccacc gcggtctggc tgattcgcct ggtccaggct cgcaacgaag
     2881 ccggaggagt tgcggatcag attccggtgc caccgcctga cgacgacacg accgcggcac
     2941 ccgacctgga cggatgagca ggccatgacc gggtccgccg catgatcgag ccccgttccg
     3001 gaacggtcac cttgctgttc gccgacatcg ccggctcgac gcgaatgctg gccgagctcg
     3061 gcgatgggta cggcgcggtg ctccgcgagt accggcggct ggtcgaggag gcggcgatgg
     3121 ctgagcacgg gtcgctgctc gataccgccg gcgatggcgt cttctactcc ttcccatctg
     3181 cgcgcggcgc cctgagcgct gcgctggccg cccagcgcag cctgcgcgac cacccatggc
     3241 ccgcggacac ccgagtcgag gctcggatcg ggatccacac gggcgagccg ctcacgagcg
     3301 acgagtcgct cgtcgggatg gacgtccacc gggcggcccg gatctgcgcg gccggtcacg
     3361 gcggccagat cctgctctcg atgacgaccc acgacctgct ccgcggcacg gcgctgcctg
     3421 acgctaccct gcgcgacctc ggagagcacc gactcaagga cctgccccac cccgagcggc
     3481 tgtaccaggc caccgcaccg gatctgccct cagagttccc agccctgcgc agcctcgaca
     3541 actggccgaa caacctgccc cgccagctgt cgagcttcgt cgggcgggcc gccgcgctgg
     3601 acgacgtgcg cgctcggctg gcggaaacct tcctgctgac cctcgttggc cccgcgggag
     3661 tgggcaagac ccgcctgggt tgcgaggtcg ccctccaggc cttggaccag ttccccgacg
     3721 gcgcatgggt ggtcgagctt ggcgcgctga ccgatggcgc gctgctcgcc gagaccgtcg
     3781 cctccaccct gcgcatcaag gagcagcccg ggacgccgtt gctccaaacg ctggccgatc
     3841 atctcgaggc ccgtcgcctg ctgctcctgt tcgacgactg cgagcacctc atcgacgcga
     3901 cggccgaggt catcgggcct ctgctccagg ctggcggcga cagccggatc ctggtcacca
     3961 gtcgcgagcc gttggccatc ccgggcgaga gcctgtatcc ggtcgcgacc ctcgagctgc
     4021 ccgcgcccga gggcgcgacg gtcgacgacg ccgccgacac cgaggcagtc cggctgttcg
     4081 tggatcgggc ggtggcggtc cagcccacgt tcgtgctgac cgccgagaac accgccgccg
     4141 tcatccagat atgccatcgg ctggacggca tcccgctggc catcgagctg gcggcggcgc
     4201 gggtcaaggc cctgccgccg gcacagatcg caagccgact ggacgaccgg ttccgcctgc
     4261 tgaccggggg cagccggatg gccctcccgc gccatcgcac cctgaaggcg gccctggact
     4321 ggagcttcga cctcctgacc gatgccgagc gcgcgctcct cgttcggtta tccgtgttcg
     4381 cgggcggttt tggcctggac gcgatcgagg aggtctgcgg cggagacgtg gtcgacaccg
     4441 atgacgtcct ggacctgctg acccggttga tcgaccggtc cctggtggtc gcggagatct
     4501 cggccggcga ggcgcgctat cgcctgctgg actcggtcca gcagtacgcc gccgagctcc
     4561 ttgcggcgga tgatcccgaa ggggcgatca acgtccgcca ccaggcgttc gtggtcgagc
     4621 tggtcgagcg cctcggcgcg gccatgctgg ccggaggccc gccgggagcg gacgcgccat
     4681 tccggcagct ggtcgcggag cgcgagaacg tgcgcgccgc ccttcaacgc gccgacgacg
     4741 acccgtccgc cgcggcccag gagctgcgcc tggccgccaa ccttggccga tactgggaga
     4801 tcgggggact gctgatcgag ggtcgcagct ggctgacccg cgccctggct cgaaccgacg
     4861 gcgaggtatc cgatctccgc gccaatgcgt tgacctcact cggcaccctc gccatccagc
     4921 agggcgactt cgcggcggcc accaccgcgc acgagaaggc gctcgccacc cagcgacagc
     4981 tgaacaatcc gaacgcggtg gcctacgccg ccagcaacct ggccaattcg tgggcggagc
     5041 gcggtgagct cgaccgcgcg cgggagctgt atgaggaggc gctggccatc ctcgccgcgt
     5101 tgagcgatcc gcgtgcatca gcccttgcca agctcaacct ggccgatgtc gccgatcggc
     5161 agggccacca ggacgaagcg cgacgcctgg tcgacgaaag cgtggagacg ttccgggccg
     5221 agcgtgaccc tatcggtatc gccctcagcc aggggcgggg ggcaacgttc agcctccgcc
     5281 acggtgaccg tgccgacgcg cgtcgccggc acgatgaggc gctcgagatc taccggatgt
     5341 tcggtgacgg gcgcggcgtg gcgcggaccc tcatgttcat cggcgacatc gtcgcgctcg
     5401 agggcgatat ggacgaggcg gagcgcctgt accgggaggc catcgaccag cggtgggcgc
     5461 tcggcgaccg cggtgggctg gcgacggcct gcgaccggct ggcccgggcc tcggccttgg
     5521 ccgatcctga acgatccgcg cggctgatcg gcttcgccga cgcccagcgc gatgcgatcg
     5581 gcgcctcgct cccgtcccag gacgccaccg aacgcgacga gttgatcggg tccctcgatg
     5641 gtcggcttgg cacggctgaa ttcggaggtc tccgccacgc tgggcgacgg ctccccctgg
     5701 tcaccgtcct gctgccgagc cccgcgccgg cgggtggatc gggagcctag gctccctccg
     5761 tgacttcctg gatgaggtcg gcgaggagac cgtgggcctc gcgatggacc tcttcggcag
     5821 cctcggcact cggcgcctcg accaggcagt acaccttgcc agtcgcctcg tcgtaccagt
     5881 agcggagata cgagacgttg tacttgccct gcacctcgag gtcgcgggcg tgcgcctggg
     5941 cgacggcgtc cccggtcagg ccggcgacgt ggttatgggt gtcaagatag aggggcatgc
     6001 tggaaggcgt ctcccggtgc gtggtcgcga cgcaacgcat tcgcggccac gaatggtagc
     6061 cctcgcctga tcgatgcgca gaccaacgcg cgacgaggtc catgtctttc cgacgacggc
     6121 cgatttccgc gcttggctgg aggcgaacca cgccacggcc gacgagctgt gggtcggcta
     6181 ctaccgcaag agcaccggca agaccgcgat gacctatccg gaggcggtgg aggaggcgct
     6241 ctgcttcggc tggatcgacg gtctcacgtt ccgcatcgac gacgaggtcc acgccaatcg
     6301 gtatacccgc cgccggaagg gcagcagctg gagcgcgccg aacatcgcca aggttgccga
     6361 gctcgccgcc gcgggccgga tgcatccggc aggggcgcgc gcattcgagg aacgcgatcg
     6421 caaaaaggac gcgccgtaca tgcgcgacca tccgctgcgg cagcagctgc cggccgagct
     6481 cgaggcccgg atcgcggcca acccggccgc ctgggcgtat tggcaagcgc aacggcccgg
     6541 attccggcgt ggggcagcgt tctggatcca gtccgccaag caggaaacga cccgggagcg
     6601 gcgcctggcc tccctgatcg aggaatcggc ggccggacgc gtgcccaacg cgctccgtcg
     6661 gccgggcgag gaccagtgac cgccggcccg gtgtcgtagg ctgcgcccgt gaccacggcc
     6721 atcagcgttc cacccggcgt cgaggtgacg cgcaccggcg gcgcgcacca cgaggaggtc
     6781 ctgaccgcgc atgccctggc cttcgtcgcc gggctccacc ggtcgttcgc ggtccgtcga
     6841 cagcacctgc tgacggatcg agccgcgcgg caggcccggt tcgatgccgg cgagcggccg
     6901 gacttcctgc ccaacacggc gcacgttcgc ggcgaccttg ggtggcgggt cgccccggcg
     6961 ccggcggacc tcgatgaccg gcgggtggag atcaccggcc cggtcgaacc gaagatgatg
     7021 atcaacgccc tgaactccgg cgcgcgggtg ttcatggccg atttcgagga cgccctctcc
     7081 cccacctggg aaaacgtcgt gaccggtcag tgggccgttc gcgaggcggt tcggcggcgg
     7141 ctgaccttcc agaccgatga aaaggcgtac gcgctgaacc ccgagatcgc gacactcgtg
     7201 atccgacctc gcggctggca ccttgacgag gcgaacgtgc tcgtcgacgg cgccccgatc
     7261 tcggccagcc tgttcgactt cggcctgatg ttcttccaca acgctcgcga gcagctcgtt
     7321 cgcggctccg gtccgtactt ctacctcccg aagctggaat cccatctcga ggccgcgctg
     7381 tggaacgacg tgttcctcgc cgcccaggcc gcgctcgaca tcccgcgcgg gagcatccgg
     7441 gcgaccgttc tgatcgagac catcctcgcc gcattcgaga tggaggagat cctgtacgag
     7501 ctccgcgagc acgcggcggg cctgaacgcc ggccgatggg actacatctt cagcatcatc
     7561 aagaagttcc gctcacggcc cgagatggtg ctccccgacc gcgcgcaggt gaccatggct
     7621 gtgccgttca tgcgcgccta ccagcagctg ctggtccaga cctgtcaccg acgtggcgcg
     7681 cacgcgattg gcggcatgag cgccttcatc cccaaccggc gcgagccgga ggtcaccgag
     7741 aaggcgctcg accaggttcg ggccgacaag gagcgggagt cgggcgacgg gtcggacgga
     7801 acgtgggtgg cgcacccgga tctcgtgccg ctcgtgcggg agatcttcga cggtgtcctg
     7861 ggcgaacggc caaaccagaa ggaccacctt ccggaagcca tcgtgacctc ggacgagctc
     7921 ttgaacgtgg aggtcgccgg cggccgcgtg accgaggtcg gcgtccggac gaacatcacc
     7981 gtcgccctcc agtacctcgc ctcgtggctg gccggcaacg gggcagccgc catcaacaac
     8041 ctgatggagg acgcggccac ggccgagatc agccgctcgc agctgtggca gtggcgcgtc
     8101 cgcggcacgc cactagagga tggccgacca atgaccgcgg aggtctacgc ctcgatccgt
     8161 gacgaggagc tgagccggct cgaagcggac atgcccggct tccgctggac cgatgccgcc
     8221 tcacttctcg acgatctcgt cctgtccgac gacttcgccg agttcctcac cacggctgcc
     8281 tatccgaggc tcggctgacg gcacgaccgc tccaccgccc attcgcccga gattgcgggc
     8341 ctcgtccagg gtgaaatggc cgcgtcggct ccggatccag gtccgcgcgt accggtaccg
     8401 gagcgcctcg taggcgatca gcgcgacaca cacggccgtg accaacgcga gcgcggcgag
     8461 ggcgggcacg accgtggcca ctggagtcag tccgatcagc acgatcgtag cgattggtcg
     8521 accgcgcccc aggcccccac cgatccgcac ccgcgtcgcc acgtgggcga ggaagtacag
     8581 cgcgaggccg ccacacaggc caacggccgg aatggcgagc aagggttcgc tgacgtgagc
     8641 aatggtcgtc ttcagcccga acgcgaacag gacgatgccc gcgaccatcg gcaggtgcag
     8701 gtaggagtac aggtcgcggg cgagcgcggc tcgctcggtc ccggacgcct cttccagcct
     8761 ggccaggacc atgaacgaaa cccagtcgaa gtacgaccac cagagcgtgg cgatcaccgt
     8821 gatcccgagg agggcggcga tgatcgtgcc ggcgtccagg ggttgcccgg cggcgccgat
     8881 cccgatcgcg acgacggact cgcccagggc gatgatgacc accagaccgt ggcgttcgac
     8941 gaagtgctcc ggcgagacgc gccatccgcg cgcgcgaccg accagggcgc cgaggtagtc
     9001 gatcgcgagc gcggcgaccc acagcgcaat ctgcgcggct ccgtcgagga aaccggccac
     9061 gaccagcatg agcgggccgg cgatggccgt cggcgtgatt cgcaggacgg cgcccagcag
     9121 ctccgggtcg ccctttcccg caatggcgta caggacgagg tggagggcgc gcaccgcaaa
     9181 gtacgcgatc ccgaagacga cgccctcggt gccgaaggcc tcgggtgtgg cgagcgcgac
     9241 gatgagcatg gccccgatcg ccgcgaacac cgcgaggcga acaacgcctt cctccgggtt
     9301 gagcgtgttc gtgagccagg cgtaggcggc ccacgcccac cacagggcgg cgagcagcag
     9361 caggccgcgg aacagcccgc cggccgtcgg gtcatcggcc aggaacccgg tgacctgggt
     9421 gatggcgaag acgaagacga gatcgaagaa cagctcgagc ggcgtgaccc gttgaccgga
     9481 ttcgatctct ggctcgttca tcagccgcct cgcatcgaac cccctgcgcc gccgggcagt
     9541 gatcgctcga ggaaccgacc tcgcctccgc tccccgagga actctcccgc ctcgacgaca
     9601 gctcggcctc gaacgaggac cgagcggaca ctgcccggga cggccatgcc ctcgaacggg
     9661 gtgaagtcgc tcgtgtggtg gaggtcgtcc tggtcgatca cccgatccgc ggccgggtcg
     9721 aacaggacga ggtccgcatc gcgaccgacc tcgatcg
//
LOCUS       DASYGU010000017         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1752291, whole genome shotgun sequence.
ACCESSION   DASYGU010000017 DASYGU010000000
VERSION     DASYGU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752291"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VGO32_00465"
     CDS             <1..387
                     /locus_tag="VGO32_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199256.1"
                     /translation="PDRGSRVEQKILHLLANVTADGIQPLAEVVAANLPQFRRGMTVG
                     IVTGATTRDWVRPLASLGRRGVGAIVVLLDLDSFATAARDEPGSRAGEISAVRHALNE
                     YGIANVLMHAGDAPERVLVTKERVRA"
     gene            380..2695
                     /locus_tag="VGO32_00470"
     CDS             380..2695
                     /locus_tag="VGO32_00470"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEV7199257.1"
                     /translation="MLDEPIEERRQIPGPREGWMSVVLLGVMLLSLCWAIQAAGWLEQ
                     LEFLVPVALFGLVAGLAFGTSRLSIAIAIPISAAFGAGIILWSVGGEFHPELSQLSRL
                     DQLRAAGIDAALTAYRYGAIQQGIVLAVVMGILMWVTAYTAAFAVYRRHQVLDAILMV
                     GAFLVINLVATVRDLFGYLVLFALAALLLWLRAALVERRSSWQLRRVSENLDVPASIM
                     RSGVLFTAVAIGLAWLLTSVAVAAPLTSAVKSLDQLWLDFAAEASGFFQGLNSANARP
                     VSSGFGPSMTVGPSWSVSDDPVLTLIGAERPYYLAAVTYDHYTGRGWSWTDAHQRSVD
                     AEADVFPAWTPDRPLIEEGFQPVTVTVQLLSPQGRDLYAPGFPVRFFAPTVVTEPGTG
                     PIFGGIEAAGSLETGQMYDVTAIVSSDVTEAQLASAPTDYEPEVTALYLDTTGITDRT
                     REMATTVVSDVPADPYHQAKALATFLRSDAFEYDTSVILPQDASQDMVDYFLWESRRG
                     FCTYYASAMVMMARSLGIPARLTVGYGPGSGVEDGSFLVTQKDAHAWAEIYFPGYGWQ
                     IFESTRSINPRFSRASGDPNAVIPVPDRSGVDFQGPFPPGYDSPTKLQGQGPTASFAP
                     IPGGFQAGEESPAEEARATNGWVFLVLIGLAAGFGVFRWFSARRRFRFMSPGDRGWAR
                     LNLAARRAGIGRQPSETFYEYAGWLEAELPSRAEEIRTIADGKVWSTYSGRSMSERAI
                     EAIERAWDRLRFPLTSLAIRRRLATFFGRPV"
     gene            complement(2697..>3195)
                     /locus_tag="VGO32_00475"
     CDS             complement(2697..>3195)
                     /locus_tag="VGO32_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV7199258.1"
                     /translation="DFAEARALAAKGQTPWTPAVSVLYGLRLGVAALVDEGPARTWAR
                     HAAIAAGASAGLEALGFRLVATPETRSATVTAAWLPDGLEWVAFDDALRARGLIVAGG
                     QGKWAGQILRFGHMGDVTWDEIDAALATTAEALTELGRSADGVAAREAGRAAYESAAR
                     AVVAA"
BASE COUNT          432 a         1153 c         1096 g          514 t
ORIGIN      
        1 cctgaccgcg gctcccgggt ggagcagaag atcctccacc tcctggccaa cgtcacggcc
       61 gacggtatcc agcccctggc cgaggtcgtg gccgccaacc tcccgcagtt ccggcgcggg
      121 atgaccgtag gcatcgtgac cggcgccacg acccgcgact gggtgcggcc gctcgccagc
      181 ctcggacgac ggggggtggg cgcgatcgtg gtcctcctcg accttgactc gttcgcgacc
      241 gctgctcgtg acgagcccgg gagtcgggcg ggggagatca gtgccgtgcg ccacgcgctg
      301 aacgagtacg gcatcgccaa cgtgctcatg cacgccggag acgcgcccga gcgggtgctc
      361 gtgaccaagg agcgggtccg tgcttgacga gccgatcgag gaacgccgcc agatccccgg
      421 accacgcgaa ggctggatga gcgtcgtcct gctcggcgtc atgctgctca gcctgtgctg
      481 ggcgatccag gccgccggtt ggctcgagca gctggagttc ctggtgccgg tcgccctgtt
      541 cgggctggtg gccggcctcg cgttcggaac gagccggctt tcgatcgcga tcgcgatccc
      601 gatctcggcc gcgttcggag ccgggatcat cctgtggtcg gtcggcggcg agttccatcc
      661 cgagctgagc cagctgtcac ggctggacca gctccgggcc gcgggcatcg atgccgcgct
      721 cacggcgtac cgatacggcg ccattcagca ggggatcgtg ctcgcggtcg tgatggggat
      781 cctgatgtgg gtgaccgcct ataccgccgc gttcgcggtc taccgccggc accaggtgct
      841 cgacgcgatc ctcatggtcg gggcattcct ggtcatcaac ctggtggcca ccgtccgcga
      901 cctgttcggc tacctcgtgt tgttcgccct cgcggccctg ctcctgtggc tccgcgcggc
      961 cctcgtcgag cggcgctcga gctggcagct gcgccgggtg agcgagaacc tcgacgtgcc
     1021 ggcttcgatc atgcgcagcg gcgtgctgtt caccgcggtc gccatcggcc tggcgtggct
     1081 ccttacctcg gtcgcggtcg ccgccccgct cacctcggcg gtcaagagcc tggaccagct
     1141 gtggctcgac ttcgccgccg aggccagtgg ctttttccag ggcctgaaca gcgcgaacgc
     1201 gcgtcccgtg tcatccggat tcgggccgag catgaccgtc ggtccgtcgt ggagcgtcag
     1261 cgacgacccg gtcctcaccc tgatcggcgc cgagcggccg tactatctgg cagccgtgac
     1321 ctacgaccac tacaccggcc gcggctggtc gtggactgat gcccatcagc gctcggtgga
     1381 cgccgaggcg gacgtcttcc cggcctggac gccggaccgt ccgctcatcg aggagggttt
     1441 ccaaccggtg acggtcacgg tccagctcct cagcccgcag ggccgcgatc tgtatgcccc
     1501 gggcttcccg gtccgcttct ttgcgccaac cgtggtcacc gagccgggca ccggcccgat
     1561 ctttggcgga atcgaggcgg cgggctcgct cgagaccggc cagatgtacg acgtcaccgc
     1621 gatcgtgtcc tccgacgtga ccgaggcgca gctggcttcg gcgcccaccg actacgagcc
     1681 cgaagtgacc gcgctgtacc tcgacacgac cggcatcacc gaccggaccc gcgagatggc
     1741 aaccaccgtc gtgtcggacg ttccagcaga cccgtaccac caggccaagg ccctggccac
     1801 gttccttcgg agcgatgcct tcgagtacga cacctcggtc atcctgcccc aggacgcctc
     1861 gcaggacatg gtcgactact tcctctggga gtcgcggcga gggttctgca cgtactacgc
     1921 ctcggcgatg gtgatgatgg cccgcagcct ggggattccg gcccgactga ccgtgggcta
     1981 cgggccgggc tcgggagtcg aggatgggtc attcctcgtc acccagaagg acgcccacgc
     2041 ctgggccgag atctacttcc cgggctacgg ctggcagatc ttcgagtcga cccgctcgat
     2101 caacccgcgg ttctcgcggg ccagcggtga ccccaacgcg gtcatcccgg tccccgacag
     2161 gagcggcgtg gacttccaag gccccttccc gccgggctac gactcgccga ccaagctcca
     2221 gggacagggc ccgactgcca gcttcgcgcc gatccccggt ggcttccagg ccggcgagga
     2281 atcgccggcc gaggaggcgc gggccacgaa tggctgggtc ttcctcgtgc tgatcggcct
     2341 ggccgccggg ttcggcgtat tccgctggtt ctcggcgcgc cgtcgattcc ggttcatgtc
     2401 acctggtgac cgcggctggg cccggctcaa cctcgccgcc cgacgggcgg gcatcggccg
     2461 gcagccgtcg gagacgttct acgagtacgc cggttggctc gaggccgagc ttccgtcccg
     2521 cgcggaggag atccggacca ttgccgatgg caaggtatgg tcgacctatt cagggcggtc
     2581 gatgagcgag cgggcgatcg aggccatcga gcgggcctgg gatcggctcc gattcccgct
     2641 gaccagcctg gccatccgtc gccggctggc caccttcttc gggcggccgg tctagttcag
     2701 gccgcgacca cggcccgggc cgcggattcg tacgccgccc gaccggcttc cctggcggcg
     2761 acgccgtcag cgctccggcc cagctcggtg agggcttccg ccgtggtggc cagcgcggca
     2821 tcgatctcgt cccaggtcac gtcgcccatg tggccgaagc ggaggatctg accggcccac
     2881 ttgccctggc cgccggccac gatcagaccg cgcgcccgga gggcgtcatc gaaggcgacc
     2941 cattccaggc catcgggcag ccaggcggcg gtgacggttg ccgaccgggt ctccggcgtc
     3001 gccaccagcc ggaagccgag cgcctccagc ccggccgagg ctccggcggc gatggccgca
     3061 tgccgtgccc aggtccgagc gggtccctcg tccaccagcg cggccacgcc caggcggagt
     3121 ccgtacagga cgctgacggc gggagtccac ggcgtctggc ccttggccgc cagcgcacgc
     3181 gcctcggcga agtcc
//
LOCUS       DASYGU010000018         5331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_826372, whole genome shotgun sequence.
ACCESSION   DASYGU010000018 DASYGU010000000
VERSION     DASYGU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826372"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="VGO32_00480"
     CDS             <1..2087
                     /locus_tag="VGO32_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7199259.1"
                     /translation="FRALVERLPAALYIAGFGPEATWNYVSPRIEALLGFSAGDWMAD
                     ASLWMRQMHPDDRDRVQREEDEDWSREPGVVSASEYRMVARDGRVVWIRDEAVIIADE
                     HGVPSFFRGYIIDITAQKNAEQALRQSEEQTRRIIDTASYAYIAMGRDGSVIDWNQAA
                     EQTFGWTREEILGKPLADRIIPTDQRDPHRQGLSQFMKTGEGPVLGTRLEVNALHRDG
                     REFPVELTIWAVGTGDSASFSALVHDITLRKELERQLQHQAFHDSLTGLANRALFADR
                     LAHAMARQRARRSLAVVFLDLDDFKTVNDSLGHAAGDQLLTAVAERLRGVMRPEDTVA
                     RFGGDEFAILLEETTREAARRVAERVLAALRSPFDLQGKQVFARGSAGVAITQSGSSA
                     DDLLRHADLAMYTAKARGKGRIEFFEPRMHLAVIRRQELRTELEHAISRQDFVLHYQP
                     FVDLRNGRLTGLEALVRWRHPERGLIEPAEFIPAAEETGLIVPLGAWVAGEATRQLAE
                     WDAAGVLPRTNGQRPLISVNVSTRQLADPGLVPMISQALKDASLEPNRLLLEITESTL
                     MADVADTEETLVLLRGIGVQLAMDDFGTGYSSLSYLERLPVDLLKIDRSFTASLGDDR
                     EIPVLLRSIIKLGQTLGLQVLAEGIERADQVVRLRELGCRLGQGYHFSPPLPADAVPE
                     AVAHHWESALTA"
     gene            2216..2437
                     /locus_tag="VGO32_00485"
     CDS             2216..2437
                     /locus_tag="VGO32_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199260.1"
                     /translation="MTTITAPTTNTGSVLIGAIVGAAAMLILMAVIALAAVNVPALLA
                     EPAPAPAPMTNAANSDDPTPLSGILEFAD"
     gene            complement(2523..5207)
                     /gene="acnA"
                     /locus_tag="VGO32_00490"
     CDS             complement(2523..5207)
                     /gene="acnA"
                     /locus_tag="VGO32_00490"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEV7199261.1"
                     /translation="MTTQPLPDPSAARATLRVGDRSYRYYDLTALGIPDLDRLPYTLK
                     ILVENAARHAASRSGLVTADDLATLAAWRPDTDPGTTELPFLPARVILQDFTGVPCVV
                     DLAAMRDAIAEMGGDPDRVNPLVPADLVIDHSVQVDQFGSDAAFLINVEREYERNGER
                     YALLRWAQGAFANFRVVPPGTGIVHQVNLEYLADVVTVRPDPDGTLTAFPDTLVGTDS
                     HTTMINGLGVVGWGVGGIEAEAALLGQPLYQPLPAVVGFRLDGALRPGTTATDLVLTV
                     TEMLRAHGVVGKFVEFHGAGLSRLGLADRATISNMSPEFGATATLFPIDDETLRYLRM
                     TGRSDEVVARVEAYAKAQHLFRTDASPEPMFSESLFLDLAHVVPSLAGPKRPQDRVSL
                     PGLPDNFRTAYADALDRSATVRLNGTTDSIRSGSVLIAAITSCTNTSNPSVMVAAGLV
                     AQKAVARGLTVPKWVKTSFAPGSKVVPEYLGAAGLMDPLEALGFHLVGFGCTTCIGNS
                     GPLAEPIAAAVGETDLVGVSVLSGNRNFEGRIHPLTRASYLASPPLVVAFALAGRIDL
                     DLTTEPLGVDADGASVFLSDLWPTVEEVDAIVAERVRSDIFRREYASVFDGDDQWRAL
                     PVPAGGRYAWDASSTYVARPPFFDGLGAEPAPLTDIEAARVLAWMGDSVTTDHISPAG
                     SIPQSSPAGKWLIEHGVEPRDFNSYGARRGHHEVLARGTFANIRLRNRLVPDAEGNVT
                     EHFPSGERTTIYEASQRYQATGTPLVVLAGREYGSGSSRDWAAKGPLLQGVRAVIAES
                     YERIHRSNLLGMGILPLQFLPGETGESLGLTGREALTIRGIAEGLSPRQQLSIEARAE
                     DGTVRTFAAVARLDGPVDVEYYRQGGVLQTVLRRLATD"
BASE COUNT          848 a         1914 c         1821 g          748 t
ORIGIN      
        1 ccttccgcgc cctcgtcgaa cggctgccgg cggcgctcta catcgccgga ttcggtcccg
       61 aggcgacctg gaactacgtg agcccccgga tcgaggccct gctcgggttc tccgccggag
      121 actggatggc cgacgcgagc ctgtggatgc gccagatgca ccccgatgac cgcgaccggg
      181 tccagcgcga ggaagacgag gactggagcc gagagccggg cgtcgtgagc gcgagcgagt
      241 accgcatggt cgcgcgcgac ggacgcgtgg tgtggatccg ggacgaggcg gtgatcattg
      301 ccgacgagca cggcgtcccc tccttcttcc gcggctacat catcgacatc accgcccaga
      361 agaacgccga gcaggcgctc cgccagagcg aggagcagac ccgccggatc atcgacaccg
      421 cgagctacgc gtacatcgcg atgggccgcg acggctcggt catcgactgg aaccaggccg
      481 cagagcagac cttcggctgg acgcgcgagg agatcctggg caagccgctc gcggatcgga
      541 tcattccgac cgatcagcgg gacccgcatc gccagggact cagccagttc atgaagaccg
      601 gggagggacc ggttctcggc acccggctgg aggtcaacgc cctccaccgc gacggccgcg
      661 aattcccggt cgagctgacc atctgggcgg tgggcaccgg cgattccgcc agcttctcgg
      721 cgctcgtcca cgacatcacc ctccgcaagg agctcgagcg ccagctccag caccaggcct
      781 tccatgactc gctgaccggc cttgccaacc gcgccctgtt cgcggaccgg ctcgcgcacg
      841 caatggcccg ccagcgggcg cgccgctcgc tggccgtcgt gttcctcgac ctggacgact
      901 tcaagacggt caacgacagc ctgggccacg ccgcgggcga ccagctcctg accgcggtcg
      961 cggagcgcct gcgcggcgtg atgcgaccgg aggacaccgt ggcccgcttc ggcggcgacg
     1021 agttcgcgat cctgctcgag gagacgaccc gcgaggccgc gcggcgggtc gccgagcgag
     1081 tcctcgccgc cctccgctcc ccgttcgacc tccagggcaa gcaggtgttt gcccgtggca
     1141 gcgccggcgt ggcgatcacc cagtccggct cgtcggccga tgacctgctc cgccacgccg
     1201 acctcgccat gtacaccgcc aaggcgcggg gcaagggccg gatcgagttc ttcgagcccc
     1261 gcatgcacct ggccgtcatt cgccggcagg agctgcggac cgagctcgag cacgcgatca
     1321 gccgccagga cttcgtgctc cactaccagc cgttcgtgga tctccgcaac gggcggctga
     1381 ccggcctcga ggcgctcgtc cgctggcggc acccggagcg cggcctgatt gagccggccg
     1441 agttcatccc ggctgccgag gagaccgggt tgatcgtgcc cctgggagca tgggtcgccg
     1501 gcgaagccac ccgacagctc gccgagtggg acgccgcagg ggtgctcccc cgcaccaacg
     1561 gccaacggcc gctgatcagc gtgaacgtgt ccacccggca gctcgcggat ccaggcctgg
     1621 tcccgatgat cagccaggcc ctcaaggacg ccagcctgga acccaaccgc ctcctgctcg
     1681 agatcaccga gagcacgttg atggcggacg tcgccgacac cgaggagacg ctcgtcctgc
     1741 tgcgcgggat cggcgtccag ctggcgatgg acgacttcgg aacgggatac tcctcgctga
     1801 gctatctcga acggctgccc gtggacctgc tcaagatcga ccgctcgttc accgcgtcgc
     1861 tcggtgacga tcgcgaaatt ccggtcctcc tccgctcgat catcaagctg ggccagacgc
     1921 ttggtctcca ggtgctggcg gagggcatcg agcgcgcgga ccaggtcgtc cggctgcgcg
     1981 agctcggctg ccgcctgggc cagggctacc acttctcgcc gcccttgccg gccgacgccg
     2041 ttcccgaggc agttgcccac cactgggaga gcgcgctcac cgcctgaccc aagcgccggc
     2101 ggcctccgct gtcggcagac cgacaccgac acggtcgccc actcgctatc ggcccgctgc
     2161 gcgcccccgc atcctgcggt catcaacttc agccgccaat tcggagacca gaccaatgac
     2221 caccatcacc gcccccacca ccaacaccgg cagcgtcctg atcggcgcca tcgtcggcgc
     2281 agccgcgatg ctgatcctca tggccgtgat cgccctggcc gccgtgaacg ttccggccct
     2341 cctggcagag ccggccccgg cccccgcacc catgaccaac gccgccaaca gcgacgaccc
     2401 caccccgctc tccgggatcc tggagttcgc tgactgacgc ttccgctctg gccctccgag
     2461 ccgcgggaag tacgagaccc gggagcaatc ccgggtctcg gcctgtcagt cgcccgacat
     2521 ggtcagtcag tggccaggcg ccggagcacg gtctggagta caccaccctg gcggtagtac
     2581 tccacgtcaa cgggcccatc gagccgagca acggccgcga aggtccgcac ggtcccatcc
     2641 tcggcccggg cctcgatcga cagctgctgg cgcggcgaca gcccctccgc gatcccgcgg
     2701 atggtgaggg cctcccgccc cgtgaggcca agcgactcgc cggtctcacc tgggaggaac
     2761 tgcagcggca ggatccccat ccccagcagg ttggagcgat ggatccgctc gtatgactcg
     2821 gcgatcaccg cccgcacgcc ctgcaggagc ggccccttgg ccgcccagtc gcgcgaggag
     2881 cccgagccgt actcgcggcc ggcgagcacc accaacgggg ttccggtcgc ctgataccgc
     2941 tgcgaggcct cgtagatcgt cgtccgctcc ccgctcggga agtgctcggt cacgttgccc
     3001 tcggcatcgg ggacgagccg gttgcggagc cgaatgtttg cgaatgtgcc gcgcgccagc
     3061 acctcgtggt gcccgcgccg cgccccatac gagttgaagt cgcgcggttc gacgccatgt
     3121 tcgatcagcc acttcccggc cgggctgctc tgcgggatgg accccgccgg cgaaatgtgg
     3181 tcggtggtga ccgaatcccc catccaggcc agcacccggg ccgcctcgat atcggtcagc
     3241 ggcgccggct cggcacccag cccgtcgaag aacggcggcc gcgcgacgta ggtcgaggac
     3301 gcgtcccagg cgtaccggcc acccgcaggc accggcagcg cgcgccattg gtcgtcgcca
     3361 tcgaagaccg atgcgtactc gcggcggaag atgtccgatc gcacccgctc ggcgacgatc
     3421 gcatcaacct cctcgacggt gggccacagg tcggagagaa agaccgatgc gccatcggca
     3481 tcgacgccca gcggctcggt agtgaggtca aggtcgatcc gcccggccag cgcgaacgcg
     3541 accaccagcg gcggtgaggc caggtacgag gctcgggtca gcggatggat gcggccctcg
     3601 aagttccggt tgccggacag gaccgatacg cccacaaggt cggtctctcc caccgcggcc
     3661 gcgatcggct cggcaagcgg gccggagttg ccgatgcagg tcgtgcagcc gaatccgacc
     3721 aggtggaagc caagcgcttc caggggatcc atcaagccgg ccgctccgag gtattcgggc
     3781 accaccttgg atccgggcgc gaacgaggtc ttcacccatt tcggcacggt cagcccgcgg
     3841 gcgacggcct tctgggccac aaggccggcg gcgaccatga ccgatgggtt cgaggtgttc
     3901 gtgcacgagg tgatggcggc gatgagcacc gagcccgacc gaatcgaatc ggtcgtcccg
     3961 ttcagccgca cggtggcaga tcggtccagg gcatcggcgt aggccgtccg gaagttatcc
     4021 ggcaggccgg gcagcgagac ccggtcctgc gggcgcttcg gcccggccag cgaggggacg
     4081 acatgggcca ggtccaggaa gagcgactcg ctgaacatcg gctcggggga ggcatcggtt
     4141 cggaacaggt gctgcgcctt ggcatacgcc tcgacccggg cgacgacctc gtccgaccgc
     4201 ccggtcatcc gcaggtagcg gagcgtctcg tcgtcgatcg ggaacagggt ggcggtggcc
     4261 ccgaactcgg ggctcatgtt gctgatcgtg gcccggtcgg ccaggcccag gcgcgacagg
     4321 cccgcgccgt gaaactcgac gaacttgccc accacgccgt gggcgcgaag catctcggtc
     4381 acggtcagga ccaggtcggt ggccgtggtg cccggacgca acgccccgtc gagccggaag
     4441 cccacgaccg cggggagtgg ctggtacagc ggctggccca ggagcgcggc ctcggcctcg
     4501 atcccaccca cgccccagcc gacgacgccc aggccattga tcatggtcgt gtgggaatcg
     4561 gtcccgacca gcgtgtcggg gaacgcggtc aacgtgccgt cggggtcggg gcggacggtg
     4621 acgacgtcgg ccaggtactc gaggttgacc tggtggacga tgccggtccc cggcggaacg
     4681 acccggaagt tggcgaacgc gccctgcgcc cagcgcagga gggcgtagcg ctcgccgttg
     4741 cgctcgtact cgcgctcgac gttgatcagg aacgcggcgt ccgacccgaa ctggtccacc
     4801 tggaccgagt ggtcgatgac gaggtccgcg gggacgaggg ggttgacccg gtccgggtcg
     4861 ccgcccatct ccgcgatcgc gtcgcgcatg gccgcgaggt ccacgacgca cgggacgccg
     4921 gtgaagtcct ggaggatgac ccgggccggg aggaacggca gctcggtcgt gcccggatcg
     4981 gtgtccggtc gccatgccgc cagcgtggcc aggtcgtcgg cggtgaccag gccactccgc
     5041 gatgccgcgt gccgggcagc gttctcgacc aggatcttga gcgtgtaggg cagacgatcc
     5101 aggtccggga tgcccagggc ggtgaggtcg tagtaccgat acgagcggtc cccgacgcgc
     5161 agcgtcgcgc gcgcagccga aggatcgggt aatggttggg tcgtcacgat cgcgcctcct
     5221 cagtcacggc tgatcggcgc cggacgggtg cgcccgtccc gggtcgagat tcgccttcga
     5281 gtatacgccg cacgattcca gcccaggcga gagtcgaccg tcaagtctgg a
//
LOCUS       DASYGU010000019         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1546663, whole genome shotgun sequence.
ACCESSION   DASYGU010000019 DASYGU010000000
VERSION     DASYGU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546663"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="ybeY"
                     /locus_tag="VGO32_00495"
     CDS             complement(<1..512)
                     /gene="ybeY"
                     /locus_tag="VGO32_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV7199262.1"
                     /translation="MEPGVTLHEKVGRPRLIEAVEPLPAETALEIATAAVAETVRGAL
                     STREAEVEVTLCGDVEMTRLNFDHMGERGPTDVLSFPLHEWSVERGHSHLADEDGRLP
                     ERGPLVLGDVVIDLDQAVRQAAEGDWSVAEEIALLAIHGTLHLLGHDHDDAEGETAMR
                     EAEHEALTVL"
     gene            complement(563..2665)
                     /locus_tag="VGO32_00500"
     CDS             complement(563..2665)
                     /locus_tag="VGO32_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEV7199263.1"
                     /translation="MFITRTLAPRDRTVTGATVARLVAVTALVGVALFAVLAIDLIPN
                     TVSLDAGDVAPEDILAPRAVSFVSTSETEAARADAEADVEPIYVEIRPQAEIRSRQLA
                     AYDAEVTAVQAILADRDAGNLSPAETQDGLTEAAPTLRASQVAILASLPTARWNAVAA
                     EARRVLDQAQAGAVREDALAEARQGAREAVSVDLAPGEREMAGDLAADHIAANLAFSS
                     EATAAARQEARAAVEPVRVTVQADEAIVRAGDPITTLDLEKLTELGLTEPEGELLPLI
                     GYSLMAALLAILLIGFLWRFQPAIWHRPRSLTLFLLILLVSAIAIRLAGDGTIVAYLV
                     PTSAAVMLTGLLLGGAAGGAMAIGLAIVAGLVNAATLGPVMYVAAGGFASLVIILQAE
                     RLNAFVRAGLVLAMVNVSVVVAFGLLDGLDGTGIAQLAVAGMVNAALSVILAVGAFAV
                     LGNVFGILTVFQMLELANPANPLLRRLLLETPGTYHHSVMVGNLAERAAETIGADPLL
                     ARVAAYYHDIGKMKNPLAFIENQAGGPNVHDDLSPETSARIIAAHVRDGVDLAQEYGL
                     PPQVAGFIPQHHGTAVMGYFAGKAIQEGEVPDADAFRYPGPKPQTREAAIVMLADGVE
                     ASVRSLPRKDDADIREMVDKIVDSRLADGQLDESNLTLRDIGQIRGSFVEQLLGMYHQ
                     RISYPENVHPIERDSRSA"
     gene            complement(2678..>3098)
                     /locus_tag="VGO32_00505"
     CDS             complement(2678..>3098)
                     /locus_tag="VGO32_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV7199264.1"
                     /translation="AMKAGDAPRVSTLRMAMAANHNRQIELGHALTDTEVIEVLDRQV
                     KQRRESIELFRGGGRPELADNEESEIAILREFLPEPLSGEELERLAREAVAASGASGP
                     ADMGKVMSVLVPQTKGRADGKEVSDLVRRLLSEASAG"
BASE COUNT          446 a         1078 c         1102 g          472 t
ORIGIN      
        1 tggaggacgg tgagcgcctc gtgctccgct tcccgcatgg ccgtctcgcc ctcggcgtcg
       61 tcgtggtcgt ggccgagcag gtggagcgtg ccgtggatgg cgaggagcgc gatctcctcc
      121 gcgacgctcc agtcgccctc cgccgcctgg cggacggcct ggtcgaggtc gatcaccacg
      181 tcgcccagga ccagcggtcc gcgctctggc agtcgcccgt cctcgtcggc gaggtgcgag
      241 tggccgcgct cgacgctcca ttcatggagc gggaaggaga ggacgtcggt cggcccgcgc
      301 tcacccatgt gatcaaagtt caggcgggtc atctccacgt ccccgcagag cgtgacctcc
      361 acctctgcct cccgggtgga aagcgcgccg cgcaccgtct cggccacggc ggcggtcgcg
      421 atctcgagcg cggtctcggc tgggagcggc tccaccgcct cgatcagccg ggggcgaccg
      481 accttctcgt gcagagtgac cccgggctcc atcggtcaat cctaggccag gcccggcacc
      541 ggctcaagga gtccgctggg gctcaggcgc tgcgcgagtc ccgctcgatg ggatggacat
      601 tctccgggta gctgatccgc tggtggtaca tccccagcag ctgctcgaca aaggagccac
      661 ggatctggcc gatatcgcgc agagtgaggt tgctctcgtc cagctgtccg tcggcaagcc
      721 ggctgtccac gatcttgtcg accatctcgc ggatgtcggc atcgtccttg cggggcagag
      781 agcggaccga tgcctcgacc ccatcggcga gcatgacaat ggcggcttcc cgggtctgcg
      841 gcttgggacc ggggtagcgg aaggcgtccg catcgggcac ctcaccctcc tggatggcct
      901 tgccggcgaa gtagcccatg accgccgttc cgtggtgctg tggaatgaac ccggcgacct
      961 gcggcggaag gccatattcc tgggccaggt ccacgccgtc gcggacgtgg gcggcaatga
     1021 tccgggcgct cgtttccggg ctcaggtcgt cgtggacgtt cgggccgccg gcctggtttt
     1081 cgatgaatgc gagcgggttc ttcatcttgc cgatgtcgtg gtaataggcc gccacccggg
     1141 ccaggagcgg gtcggcgcca atggtctccg ccgcccgttc cgccaggttg ccgaccatca
     1201 ccgagtggtg atacgtcccc ggcgtctcga gcaggaggcg gcggaggagt gggttggccg
     1261 ggttggcgag ctcgagcatc tggaacacgg tcaggatccc gaatacgttg cccagcaccg
     1321 cgaacgcgcc caccgcaagg attacggaca gggcggcgtt gaccatgccg gcgaccgcca
     1381 gctgggcgat gccagtcccg tccagcccat cgagcaggcc gaaggccacg accaccgaga
     1441 cgttgaccat ggcgagcacc agcccggccc ggacgaaggc gttcagccgc tcggcctgga
     1501 ggatgatcac gagcgaggcg aacccgccgg ccgccacgta catcaccggc cccagggtgg
     1561 cggcgttcac gaggccggcc acgatcgcca ggccgatggc catcgccccg cccgcggccc
     1621 cgcccaggag gaggcctgtg agcatgaccg cggcggaggt tggcaccagg taggccacga
     1681 tcgttccgtc gccggccagc cggatggcga tggcgctgac cagcaggatg agcaggaaca
     1741 gggtgaggct ccggggccga tgccagatgg ccggctggaa acgccacagg aagccgatga
     1801 gcaggatggc gaggagggcg gccatcaggc tgtagccgat gagcgggagg agctcgccct
     1861 cgggctcggt cagccccagc tcggtgagct tctcgagatc gagcgtcgtg atcgggtcac
     1921 cggcgcgaac gatcgcctca tccgcctgga ccgtgacccg gaccggctcc accgccgcgc
     1981 gcgcctcctg gcgggcggcc gcggtcgcct cgctgctgaa cgcgaggttg gccgcgatgt
     2041 ggtcggccgc caggtcaccg gccatctccc gctcaccagg ggcaaggtcg acgctgaccg
     2101 cttcgcgggc accctgccga gcctcggcca gcgcgtcctc ccgcacggcg ccggcctgtg
     2161 cctggtccag gacccggcgg gcttcggccg caactgcgtt ccaacgcgcc gtgggcagac
     2221 tggccaggat ggcgacctgc gaggcacgga gcgtcggagc cgcctcggtc agcccgtcct
     2281 gggtctcggc cggcgacagg ttccccgcgt cgcggtcggc gaggatggcc tggaccgcgg
     2341 tcacctccgc gtcatacgcc gccagctggc gcgagcggat ctcggcctgg ggccggatct
     2401 cgacgtagat cggctcaacg tccgcctccg cgtccgcgcg ggccgcctcg gtctcggagg
     2461 tggagacgaa cgacaccgcg cgtggggcga ggatgtcctc gggcgcgacg tcgcccgcat
     2521 cgagcgacac ggtgttcggg atgaggtcga tggccagaac ggcgaagagg gccacgccga
     2581 cgagcgcggt gaccgcgacc agccgcgcca cggtggcacc ggtcacggtc cggtcccgcg
     2641 gcgcgagagt cctggtgatg aacatgggtc cgggatgcta ccccgcagac gcctcggata
     2701 acagccggcg aacaaggtca ctgacttctt tcccatccgc ccgacccttg gtctggggga
     2761 cgagcacgct catgaccttg cccatgtccg ccggtccgct ggctccgctc gcggccacgg
     2821 cttctcgggc cagtcgttcc agctcctcgc ccgacagcgg ctcgggcagg aattcgcgga
     2881 ggatcgcgat ctccgactcc tcgttgtcgg cgagctcggg ccggccgccg ccgcggaaca
     2941 gctcgatgct ctcgcggcgt tgcttgacct ggcggtcgag gacctcgatc acctcggtgt
     3001 cggtcagcgc gtggccgagc tcgatctgcc ggttgtggtt cgccgccatg gccatccgca
     3061 gggtgctgac ccgcggagcg tcgcccgcct tcatggca
//
LOCUS       DASYGU010000020         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1186802, whole genome shotgun sequence.
ACCESSION   DASYGU010000020 DASYGU010000000
VERSION     DASYGU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186802"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="prfB"
                     /locus_tag="VGO32_00510"
     CDS             join(<1..63,65..1120)
                     /gene="prfB"
                     /locus_tag="VGO32_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158066129.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV7199265.1"
                     /translation="DEIRSEAQRLARLVADTAVRLDLPTRQAELEALTTQSSDPNLWD
                     DPARAQTILRRADELRDEIAFWTDLERQATDLADLTELAEAEPDGGETMRADLQASLS
                     ALQASWAKAEHRLLLGGPYDERPAIISIYAGAGGTESQDWAEMLLRMYLRWAERQRFK
                     TEILDQSEGEEAGLKSATATIDGRWAYGRLRSERGVHRLVRISPFDSQKRRHTSFALV
                     EVMPEVPADAEIEIRDDDLKIDTYRSSGAGGQHVNKTDSAVRITHLPTGIVVTCQNER
                     SQMQNKERAMAVLRAKLVERAEEEREAELAKLRGVHVEPGWGNQIRSYVLQPYTMVKD
                     LRTGVETSDPTAVLDGDLDPFIEGYLRGQIGTDGNGAA"
     gene            1150..1833
                     /gene="ftsE"
                     /locus_tag="VGO32_00515"
     CDS             1150..1833
                     /gene="ftsE"
                     /locus_tag="VGO32_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV7199266.1"
                     /translation="MIRMVDVAVTYPNGKTALTNVDLEVARGDFIFLVGPSGAGKSTM
                     IRLLVREQVATKGRVYLAGRDLARVSRAQVHRLRRRIGVVFQDFRLLPNKTVRENVAF
                     ALEVTGAPLAEIRHRVPRLLSLVGLEDQSEQLPTQLSGGEQQRCAIARALVHDPAILI
                     ADEPTGNLDPVTGWEIVQLLLEINGLGTTLIMATHNQEIVTALRRRVVALEGGRVVRD
                     EVGAAYRRG"
BASE COUNT          322 a          675 c          696 g          282 t
ORIGIN      
        1 gacgaaatta ggagcgaggc ccagcgcctg gcgcgcctcg tcgccgatac cgcggtgcgt
       61 ctttgacttg cccacccggc aggccgaact cgaggcgctg accacccagt cgtcggaccc
      121 caacctgtgg gacgacccgg cccgggcgca gacaatcctg cggcgggccg atgagctccg
      181 cgacgagatc gccttctgga ccgatctcga gcggcaggcg acggatctcg ccgacctgac
      241 cgagctcgcc gaggccgagc cggacggggg cgaaaccatg cgcgccgatc tccaggcatc
      301 gctctccgcg ctgcaggcca gctgggcgaa ggccgagcac cggctgctgc tgggtgggcc
      361 gtacgacgag cggcccgcga tcatctcgat ctacgcgggc gcgggcggca ccgagagcca
      421 ggactgggcc gagatgctgc tccggatgta cctgcgctgg gccgagcgcc agcggttcaa
      481 gaccgagatc cttgaccaga gcgagggcga agaggcgggc ctcaagagcg ccacggcgac
      541 catcgacggc cggtgggcct acgggcggct gcggagtgag cggggggtcc atcggctggt
      601 ccggatcagt ccgttcgact cccaaaagcg ccgccacacc tcgttcgcgc tggtcgaggt
      661 catgcccgaa gtgccagccg atgccgagat cgagatccgc gacgacgacc tgaagatcga
      721 cacctatcgg tccagtggtg cgggcgggca gcacgtgaac aagaccgatt ccgcggtgcg
      781 catcacgcac ctcccgaccg ggatcgtggt gacctgccag aacgagcgga gccagatgca
      841 gaacaaggag cgcgcgatgg ccgtcctgcg ggccaagctc gtcgagcggg ccgaggagga
      901 gcgtgaggcg gagcttgcca agctccgcgg cgtgcacgtg gagccgggct ggggtaacca
      961 gattcggagc tacgtcctcc agccatacac catggtcaag gacctgcgaa ccggggtcga
     1021 gacctcggac ccgaccgccg tccttgacgg cgacctcgac ccgttcatcg agggctacct
     1081 gcgggggcag atcggaaccg atggaaacgg ggccgcgtga ccaggactgt cgccacccct
     1141 gacgagccga tcatccggat ggtggacgtg gcggtcacct atccgaacgg caagacggcg
     1201 ctgaccaacg tcgacctcga ggtcgcgcgg ggggacttca tcttcctcgt cggcccgtcc
     1261 ggcgcaggca agtcgacgat gatccggctc ctcgtccggg agcaggtcgc gaccaagggc
     1321 cgggtgtacc tcgccggccg cgacctcgcc cgggtgagcc gggcccaggt ccaccgcctg
     1381 cgccgccgga tcggtgtcgt gttccaggac ttccgcctcc tgccgaacaa gaccgtccgc
     1441 gagaacgtgg cgttcgcgct ggaggtgacc ggcgcgccgc ttgccgagat ccggcaccgc
     1501 gtgccgcggc tgctctcact ggttggtctc gaggaccagt cggagcagct cccgacccag
     1561 ctgtcgggcg gcgagcagca gcggtgcgcg atcgcgcggg ctctcgtcca cgaccctgcg
     1621 atcctcatcg ccgacgagcc gaccggcaac ctggacccgg tcaccggctg ggagatcgtc
     1681 cagctcctgc tcgagatcaa cgggctgggg acgaccctga tcatggccac ccacaaccag
     1741 gaaatcgtca ctgcgctccg ccgtcgggtt gttgcgctcg agggcgggcg cgtggtccgc
     1801 gacgaggtgg gagcggccta ccgccgcggc tagcccggta ggatgcgcgc gacatgtcgt
     1861 tcctgaggtt cctgggtttc accctggttc gggccggcca gggcttctgg cgcaaccgga
     1921 tgatgtcgct ggccgccacc gcgaccgtga tcctcatgct cgtgctgctc tcggg
//
LOCUS       DASYGU010000021         6888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_723859, whole genome shotgun sequence.
ACCESSION   DASYGU010000021 DASYGU010000000
VERSION     DASYGU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723859"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..752)
                     /locus_tag="VGO32_00520"
     CDS             complement(267..752)
                     /locus_tag="VGO32_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199267.1"
                     /translation="MTLWVVKGGRGGIREERFLARSIIGIGWGELGDLSRYPDRDTLK
                     AAYRGAHPERSENHVSTQVAQLWAFVSRMKVGDAVVVPFLGQPEIAVGEIRGPYRWIR
                     EGEPDMPHVRDVTWQVTDLPRIAFDLDLLYSFGGSVSVCSVRRNDAERRVRAMMAGRS
                     R"
     gene            complement(754..2076)
                     /locus_tag="VGO32_00525"
     CDS             complement(754..2076)
                     /locus_tag="VGO32_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV7199268.1"
                     /translation="MNEASGRWLVRLSLFGAVIGIPAALLAVVFFTLVHELEHLLWTD
                     LPAALGETTAPWYLVVGLPVVGAIIVAATRILLPGNGGHSPMDGISAIPTSPRAIPGV
                     ALAALGTLGFGLVLGPEAPVMALGSAVGVALTSLVRTTPRETGVLSTAGQFSAISSLF
                     GGPLVAGVMLTEGGIGAGRALIGALLPGFVAAALGYLIFVGVGAYTGTAPPGLTVPDL
                     EPYIGTTVPDLLMAVALGVAIAVVMVGVRRLAEWIHDGGQRLLGESNAGVTIGLVAGG
                     LAVGLLALGAGGLGANPQDVLFSGQQSIPAVVAQPSVMALVILLVAKALAYVISLASG
                     FRGGPIFPALFIGIGVADFAVQLFGMSPTLAIAVGAAAAMTAQTRLVVTSMVFSALLV
                     GSAGADAIPAAVLATVAAYVTATAFDRRTSEAAAPNIPASDATPPAAG"
     gene            complement(2153..2986)
                     /locus_tag="VGO32_00530"
     CDS             complement(2153..2986)
                     /locus_tag="VGO32_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199269.1"
                     /translation="MTTRSVGGVGGAGVVGLSVLALLFLAVPVVGLVVRAVAGGAIVE
                     AVTSPAVRDALVLSLATTAVSLALVVIFGTPLALLLARGTFRGSSVVESIVDLPIVLP
                     PAVAGLALLLMFGRSGVLGGALELAGIRIPFTTLAVVLAQTFVAAPFFIRTARTGISS
                     VDRQLQDAARDLGAEESSVFRRVTLPLAAPALATGMVLCWSRALGEFGATIMFAGNLP
                     GVTRTLPLVVYSEFQSGDLEASIAAAAILILAAFGVLVAVRRLRWGVTPTVRTGGIDR
                     P"
     gene            complement(2983..3822)
                     /gene="modA"
                     /locus_tag="VGO32_00535"
     CDS             complement(2983..3822)
                     /gene="modA"
                     /locus_tag="VGO32_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7199270.1"
                     /translation="MTRRATPRTLLAVLAMALVLAACQPSASANPSASVDEPSAAASV
                     EDVELTILGAASLQDALDEIDVEYEAAVPGTNLVIATDSSAALRTQIEEGSPADVYLS
                     ADTSNPDTLVEAGIGVETVEYAGNSVALVVPMDNPAGIESPADLATAGIQIIAAGAEV
                     PITSYVAEVVANLAGLPDYPAGFADAYAANVVSEEDNVRGVLTKIELDEGDAGFVYQT
                     DAESSADVQIIEIPEDANTHAVYGGCVISDSNRLEAAEAFLTWLAGPDGQAMLAEFGF
                     IGP"
     gene            complement(3819..4217)
                     /locus_tag="VGO32_00540"
     CDS             complement(3819..4217)
                     /locus_tag="VGO32_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEV7199271.1"
                     /translation="MEVRIGEAAEMLGVSVETLRRWEHDKQLRTVRTAGGQRSVPLAE
                     VTRLLTERRRTETDRPIVAQSARNRFPGVVTRIQRDKVVAVVEVQAGPHRLVSIMTTE
                     AVDDLDLQVGKEAVCVVKSTNVIVEVPADR"
     gene            complement(4252..4638)
                     /locus_tag="VGO32_00545"
     CDS             complement(4252..4638)
                     /locus_tag="VGO32_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV7199272.1"
                     /translation="MLPALHPRPTALLAVALLALSLVACAASDTPFETQTVTITPAPE
                     FDPADITIPVGTTVIWSNTDGVAHSIQFLDGELPTDPLMVEPGNTFEFTFQEAGLIHY
                     QCGTHPDEMTGTITVTPADLPSDPNT"
     gene            4730..5422
                     /locus_tag="VGO32_00550"
     CDS             4730..5422
                     /locus_tag="VGO32_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7199273.1"
                     /translation="MAPRSRPTEHDIDRVGLLAEPVRRQLYELVASTPEAVSRDHAAS
                     ALDIGRPLAAFHLDRLVEGGLLEVEYRRRSGRSGPGAGRPAKFYRRAADRQVDISLPA
                     RRYRLAAEILADGLDRAAEPAAMDGVRDAARATGRRLAHDAIGSQPPRAALMQVLTDG
                     GFEPFEEQGVIRLRNCPFDALVQDHRDLTCGLNLTLLESVAAELPGTGLTPVRRPRDG
                     LCCVEFVPERAT"
     gene            complement(5419..6264)
                     /locus_tag="VGO32_00555"
     CDS             complement(5419..6264)
                     /locus_tag="VGO32_00555"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7199274.1"
                     /translation="MAKLTDKQRARMPRGAFAYIDSKGVRRLPIFDEPHVRSALSRFN
                     QVQFESEAARDEARTRLLKAARKYGVVPVGFIQSQLRTERRRGQTDTASRRGAALPTG
                     VVTFLMTDVEDSTSLLSELEEGYGAVLSDIRRIIRDKVRAAGGHEVEVHADEFLGAFA
                     HAPDALAAAVAIQRRMTDKAWPTKKPVRVRAGIHRGHPTLTDGAYFGLSVHATARVCQ
                     VAHGGQVLVSRTAHEAMVELVPAEVGFTNLGEHRLRGFPRPDVLYQVTAPGLPTRFRA
                     LRTTL"
     gene            complement(6267..>6888)
                     /locus_tag="VGO32_00560"
     CDS             complement(6267..>6888)
                     /locus_tag="VGO32_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV7199275.1"
                     /translation="LSAIQPILAAHDVTVTDDDVLERYARHESGLEAGPYQSYRGVLA
                     GSLGGVADELGAEVTAEELAGFSESVADWPPFEDSTDALHRLANRCRLGVITNCDDDL
                     FAASARRLEVNFEWVVTAEQAQDYKPSIAPFVLALRALDVPKERVLHVAQSLYHDHAP
                     AKELGMTTVWVNRRHDRPGSGATPPADATPDLEVPDLASLAEQAGV"
BASE COUNT         1096 a         2363 c         2463 g          966 t
ORIGIN      
        1 attcgtcggt ggctggattg ggagcgaggc gcttggcgcg ctgagcacat ggggccccga
       61 gtttgaaggc ctgtacatcc tgcctgcgat catcggcggg atcgcgctgg gtggctttgt
      121 ggacttcttt gtccgccgcg ccaccggtgg aagctacctg gagccacggc cgatctagcc
      181 gttcgctcat tcctgttggc agtggccgtc acgacgacgg ccactgcccc tctattgggc
      241 tggccaagct aaccctgctc tgcggctcat cgcgatcttc cggccatcat cgcccgcacc
      301 cgccgctccg cgtcgtttcg ccggacggag cagacggaca ctgagccgcc gaacgagtag
      361 agcaggtcta ggtcgaacgc gatccgcggc aggtcggtga cctgccaggt cacgtcgcga
      421 acatggggca tgtccggctc gccttcgcgg atccatcggt agggaccgcg aatctcaccg
      481 accgcgatct ccggctgccc caggaaggga accaccaccg cgtcaccgac cttcatccgc
      541 gagacgaatg cccacagctg agcgacctgg gtgctgacgt gattctccga gcgctcgggg
      601 tgcgccccgc ggtaggcggc cttcagcgtg tcgcgatccg ggtagcggga caggtcgccg
      661 agctcacccc agccgatccc gatgatcgaa cgggcgagga accgctcctc gcggataccg
      721 ccgcggccgc ccttcacgac ccacagggtc atgtcagccc gcggcaggcg gcgtggcgtc
      781 cgaagccggg atgttgggtg cggccgcttc ggaggtccgg cggtcgaagg cggtggccgt
      841 gacgtaggcg gcgaccgtgg ccagcaccgc ggccgggatg gcgtcggcgc ccgccgagcc
      901 aacgagcagg gcggagaaca ccatggaggt caccaccagt cgggtctggg cggtcatggc
      961 cgccgcagcg cccaccgcga tggcgagggt tggtgacatc ccgaacagct gtaccgcgaa
     1021 gtcggcaacg ccgatgccaa tgaagagggc cgggaagatc ggcccgccgc ggaagccgga
     1081 cgccaggctg atgacgtagg ccagcgcctt ggccaccagc aggatgacga gggccatgac
     1141 cgatggctgg gcgacgacgg cggggatgga ctgctggccc gaaaacagga cgtcctgcgg
     1201 gttcgccccc agtccgccgg cgccgagggc gaggagcccc acggccagtc ccccggccac
     1261 caggccgatc gtcacaccgg catttgattc gcccaggagc cgctgcccgc catcgtggat
     1321 ccactccgcg aggcggcgga cgcccaccat cacgaccgca atagcgacgc cgagcgcgac
     1381 cgccatcagc aggtccggga ccgtcgtgcc aatgtatggc tccaggtccg gcacggtgag
     1441 ccccggcggc gctgtgccgg tgtacgcgcc cacgccgacg aagatcaggt agcccagggc
     1501 agcggccacg aacccgggca gcagggcccc aatgagcgcc ctgccggccc cgatcccgcc
     1561 ctcggtcagc atcaccccgg ccacgagggg cccgccgaac aggctcgaga tggccgagaa
     1621 ctggccggcg gtggacagga ccccggtctc gcgcggagtg gtccggacca gcgaggtcag
     1681 ggccactccc accgcggagc ccagggccat gaccggggcc tccgggccga ggaccaggcc
     1741 gaagcccagc gtgcccaggg ccgccagcgc gacaccgggg atcgcgcggg gcgaggtggg
     1801 gatggcgctg atcccgtcca tcggggagtg gccgccgttc cccggcagga ggatccgcgt
     1861 cgcggcgacg atgatcgcgc cgacgaccgg caggcccacg accaggtacc acggggccgt
     1921 ggtctcgccc agtgcggcgg gcagatcggt ccacagcagg tgctccagct cgtggaccag
     1981 ggtgaagaag accaccgcca gcagggccgc cgggattccg atcacggccc cgaagagcga
     2041 gagccgcacc agccagcgcc ctgacgcctc gttcatcgcg tctcctcgtc aggtaggtcc
     2101 gcatgatgcg cggatcggtc gacccaaatg gtcgaggtgc cgcgcgactg ggtcagggtc
     2161 ggtcgatccc tcccgtccga acggttggcg tgacgcccca gcggagtctg cggacggcga
     2221 ccagcacgcc gaaggcggcc aggatgagga tcgctgcggc ggcgatggag gcctccaagt
     2281 cgccggactg gaactcggag tacacgacca ggggcagggt ccgggtcacc cccggcaggt
     2341 tgccggcgaa catgatcgtg gctccgaact cgcccaacgc tcgggaccag cacaggacca
     2401 tcccggtcgc cagggccggg gccgccagcg gcagagtgac ccggcggaag accgatgact
     2461 cctccgcgcc gaggtctcgg gcggcgtcct gcagctgccg gtccacgctg ctgatcccgg
     2521 ttcgcgcggt acggataaag aacggggcgg ccacgaacgt ctgggccagc acgaccgcaa
     2581 gggtcgtgaa cgggatccgg atcccggcca gctcgagggc cccgcccagc accccgctcc
     2641 gcccgaacat gagaagcaga gccagcccgg ccacggccgg tgggagcacg atcggcagat
     2701 ccacgatcga ctccaccacg ctcgagccgc ggaacgtccc gcgggcgagg agcagggcca
     2761 gcggcgtgcc aaagatcacg accagagcga ggctgaccgc ggtcgtggca aggctcagga
     2821 cgagggcgtc ccggaccgct ggcgacgtga cggcctcgac gatggccccg cccgcaacgg
     2881 cacggacgac gagaccgacg accggaacgg ccaggaacag gagtgccagg accgacaggc
     2941 caacgacgcc ggcgcctcca accccgccga ccgaccgagt ggtcacggtc cgatgaagcc
     3001 gaactccgcc agcatcgcct gcccgtcggg gccggccagc caggtcagga atgcttcggc
     3061 cgcctcgagg cgattggagt ccgagatcac gcagccgccg tagaccgcgt gggtgttcgc
     3121 gtcctcggga atctcgatga tctggacatc ggccgaggac tccgcatcgg tctggtacac
     3181 gaacccggcg tcgccctcgt ccagctcgat cttggtcagg acgccgcgga cgttgtcctc
     3241 ctcggacacg acgttggcgg catacgcatc ggcgaagccg gccgggtagt ccggcagccc
     3301 ggcgaggttg gccacgacct ccgcgacgta cgaagtgatc ggcacctcgg cacccgcggc
     3361 gatgatctgg atgcccgcgg tggccaggtc ggccggggac tcgatcccag ccggattgtc
     3421 catcggtacg accagggcca cggagttccc ggcgtactcg accgtctcca cgccgatgcc
     3481 cgcctcgacc agggtgtcag ggttggaggt gtccgccgag aggtacacgt cggctggaga
     3541 cccctcctcg atctgggtcc gcaatgcggc cgacgagtcg gtcgcgatga ccagattggt
     3601 tcccggcacc gccgcctcgt actccacgtc gatctcatcc agggcgtcct ggagcgaggc
     3661 tgcaccgagg atcgtgagct cgacgtcttc gaccgaagcc gccgcagatg gttcgtccac
     3721 gctggccgat gggttggccg atgccgatgg ctggcaggca gccaggacga gcgccatggc
     3781 caggacggcg aggagcgtgc ggggggtggc tcgacgtgtc atcggtctgc ggggacctcc
     3841 acgatcacgt tcgtggactt cacgacgcag acggcctcct tcccgacctg gagatcgaga
     3901 tcgtcgacgg cctcggtggt catgatgctg accagccggt gcgggccggc ctgcacctcg
     3961 accacggcga cgaccttgtc gcgctggatg cgggtcacca caccggggaa gcggttacgg
     4021 gcggactggg ccacgatcgg tcgatcggtc tcggtccggc gccgctccgt gaggaggcga
     4081 gtgacctcgg caagcggaac gctccgctga ccgccggcgg tccgaaccgt cctcagctgc
     4141 ttgtcatgct cccatcggcg gagggtctcg accgatacgc cgagcatttc ggcggcttct
     4201 ccgatccgaa cctccatacg gaggaatgta cgccgctgga gtcaggaatg tctaggtatt
     4261 gggatccgag gggaggtctg ccggggtgac cgtgatcgtt ccggtcatct catccgggtg
     4321 ggtgccgcac tgatagtgga tcaggcccgc ctcctggaac gtgaactcga aggtgtttcc
     4381 cggttcgacc atgagtggat cggtcgggag ctcgccatcg aggaactgga tggagtgggc
     4441 cacgccgtcg gtgttcgacc agatcaccgt ggtgcccacc gggatcgtga tgtctgctgg
     4501 atcgaactcc ggcgcgggcg tgatggtgac cgtctgcgtc tcgaacgggg tgtccgacgc
     4561 cgcgcaggcc acgagcgaga gggccaggag ggcgaccgcc agcagggcgg tgggacgagg
     4621 gtgcagagcg ggcagcatca gggacgtgat ggtacgcccc ctcgcttggc cagagcacgg
     4681 tcctcccgac tggctcgctc tagaacgagt ccatactatt ggtagaccga tggcaccgcg
     4741 atcacgaccg accgagcacg atatcgaccg ggtcgggctc ctggccgagc ccgtccgccg
     4801 tcagctgtac gagctcgtcg cgagcacgcc cgaggccgtg agccgggacc acgccgcgtc
     4861 ggccctcgat atcggtcgac cgctggctgc gttccacctc gatcgcctgg tcgagggcgg
     4921 gctgctggag gtcgagtacc gacggcgttc cgggcgatct gggcccggcg cgggccggcc
     4981 ggcaaagttc taccggcgag ccgcggaccg gcaggtggac atctcgctgc cggcacggcg
     5041 gtaccgcctg gcggccgaga tcctggccga cggcctggac cgcgccgcgg agcccgcggc
     5101 gatggacggc gttcgcgacg ccgcgcgggc gacggggcgc cggctggccc atgacgcgat
     5161 tggcagccag ccgccgcggg cggcgctgat gcaggtgctc accgatggcg ggttcgagcc
     5221 gttcgaggag cagggcgtga tccggctgcg gaactgcccg ttcgacgctc tggtccagga
     5281 ccaccgcgac ctgacctgcg gcctgaacct cacgctgctg gagtcggtgg ccgcagagct
     5341 gcccggcacg gggctcaccc ccgtgcggcg gccacgcgac gggctgtgct gcgtggagtt
     5401 cgtgccggag cgcgcgacct agagcgtcgt ccggagtgcg cggaagcgcg tgggcagtcc
     5461 cggcgcggtg acctggtaca ggacatcagg ccggggaaag ccgcggagcc gatgctcgcc
     5521 caggttggtg aatcccacct cggcggggac gagctcgacc atcgcctcgt gggcggtccg
     5581 cgagacgagc acctgccccc cgtgcgcaac ctggcagacg cgggcggtgg cgtgcacgga
     5641 cagaccgaag tacgcgccat cggtcagggt cggatgaccg cggtggatgc ccgcccgaac
     5701 ccgcaccggt ttcttggtgg gccacgcctt gtccgtcatt cggcgctgga tggcgacagc
     5761 ggccgccagc gcatccggcg cgtgggcgaa cgcgccaagg aattcgtcgg catggacctc
     5821 cacctcatgg ccgccggcgg cccggacctt gtcgcggatg atgcgccgga tatcggacag
     5881 gaccgccccg tatccctctt ccagctcact cagcagcgag gtcgagtcct cgacatcggt
     5941 catcaggaag gtcaccaccc cggtcggcag ggcggccccg cgccgggatg ccgtatcggt
     6001 ctggccccgt cggcgctcgg tccgcagctg cgactggatg aatcccaccg gcaccacccc
     6061 gtacttgcgg gccgctttca ggagtcgcgt ccgggcctcg tctcgcgccg cctcggactc
     6121 gaactggacc tggttgaacc gcgacagggc cgaacggacg tgcggctcgt cgaagatcgg
     6181 cagccggcgc acgcccttgg agtcgatgta cgcgaacgcg cctcggggca tccgcgcgcg
     6241 ctgcttgtcg gtgagcttgg ccacgcctag acccctgcct gctccgcgag cgaggccagg
     6301 tccggtacct cgaggtcggg cgtcgcatcg gccggcggcg tcgcgccgga cccgggccgg
     6361 tcgtgacggc ggttgaccca caccgtggtc atcccgagct ccttggccgg ggcgtggtcg
     6421 tggtacaggc tctgtgccac gtggaggacg cgttccttgg gcacgtccag ggcccggagg
     6481 gcgaggacga acggggcgat gctcggcttg tagtcctgcg cctgctcggc cgtgaccacc
     6541 cactcgaaat tgacctccag ccgccgggcg gaggcggcga acaggtcgtc gtcgcagttg
     6601 gtgatgacgc ccagccgaca ccgattcgcc agccggtgga gcgcatcggt cgaatcctca
     6661 aatggaggcc agtccgcgac cgactcgctg aatccggcaa gctcttcggc cgtcacctcc
     6721 gcgccgagct cgtcggcgac gccaccgagc gagccggcaa ggacgccgcg gtacgactgg
     6781 tacggcccgg cctcaagccc cgattcgtgc cgagcgtagc gttcgaggac gtcatcgtcg
     6841 gtcacggtca cgtcgtgcgc ggcgaggatc ggctggatcg cggagagg
//
LOCUS       DASYGU010000022         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1649926, whole genome shotgun sequence.
ACCESSION   DASYGU010000022 DASYGU010000000
VERSION     DASYGU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649926"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VGO32_00565"
     CDS             <1..1300
                     /locus_tag="VGO32_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEV7199276.1"
                     /translation="TKHWSRAVDVNRPLIEEALRLRQRAAELLGFPTWAHFAIEVKMA
                     ETPDAVAAFYDQIVPGLAAIRDRELAVLAERLHRDGHAGEVAAWDWAYYDEQLKRDEF
                     GVDANLVAEYLPMAACLEGMFALTGEVLGLEYRRIPDPKVWHPSVELYEIRDRASGAV
                     IAEFYADWFPREGKFGHAAAFPLVIGHRNPDGSYERPVSAILANFTPPSGDRPSLIQH
                     NELETMFHEFGHILHMSLTKAENVRVSGGETEIDFVEAPSQIMEHWTYAPEVISRFAR
                     HYETREPMPAELLEQLVAARYVDIGIKTMFQVFYGQIDLGIHDGQGEQDLDAALRRAY
                     VHTGMPYPDGTFLLSGFGHPMGGYDAGYYGYLWSEVIGDDLFSRFEREGVTSPAVGAD
                     YRREILEKNGSRPAAELVHNFLGRDFTPDAWFHLRNMDQT"
     gene            complement(1297..2091)
                     /locus_tag="VGO32_00570"
     CDS             complement(1297..2091)
                     /locus_tag="VGO32_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7199277.1"
                     /translation="MAADAEELRAFALDLADAADAITLPAFLAGVAASPKPDGTPVTE
                     ADTAVEALIRTRIGQRYPTHAMLGEEFGEQPGPAGADGTRWVVDPIDGTVNFARGIQV
                     WATLIAVERDGELLASVVAAPALGQRWSAFRGGGATTSRSGVERPIRVSSVSRLEDAH
                     VVHAGLGAFWDDGRGEGAAAVLRHSARDRGIGDFWGYMLVAQGSADAMFELGVKPWDL
                     AAPALVVMEAGGRFTDLDGGENRTGPSALATNGALHAELLEALARS"
BASE COUNT          355 a          821 c          757 g          319 t
ORIGIN      
        1 caccaagcac tggagccgcg cggtggacgt caaccggccg ctcatcgagg aggcgctccg
       61 gctccgccag cgcgcggcgg agctgctggg cttcccgacc tgggcccact tcgcgatcga
      121 ggtcaaaatg gccgagacgc cggacgcggt ggccgccttc tacgaccaga tcgtcccggg
      181 cctggccgcc atccgcgacc gcgagctggc ggtcctcgcc gaacggctgc atcgcgatgg
      241 ccacgcgggc gaagtcgccg cctgggactg ggcctactac gacgagcagc tcaagcgcga
      301 tgagtttggg gtcgacgcca acctggtcgc cgagtacctg cccatggcgg cctgcctgga
      361 gggcatgttc gccctcaccg gcgaggtgtt gggcctcgag taccgccgca tcccggaccc
      421 gaaggtctgg catccatcgg tcgagctgta cgagatccgc gaccgcgcca gcggcgccgt
      481 catcgccgag ttctatgccg actggttccc acgcgagggc aagttcgggc atgcggccgc
      541 gttcccgctg gtcatcggcc accggaaccc ggacgggagc tacgagcgac cggtgagcgc
      601 gatcctggcc aacttcactc cgccgtcagg cgatcggccc tcgctcatcc agcacaacga
      661 gctggagacg atgttccacg agttcgggca catcctccac atgtcgctga ccaaggcgga
      721 gaacgtgcgg gtcagtggtg gggagaccga gatcgacttc gtggaggctc cgtcccagat
      781 catggagcac tggacgtacg caccggaggt catcagccgg ttcgcgcgcc attacgagac
      841 ccgtgagccc atgccggccg agctgctgga gcagctggtg gccgcgcggt acgtggacat
      901 cggaatcaag accatgttcc aggtcttcta cggccagatc gacctgggga tccacgacgg
      961 ccagggggag caggacctcg acgcggccct tcgccgcgcg tacgtccaca ccggaatgcc
     1021 gtacccggat ggcacgttcc tgctgtccgg cttcgggcac ccgatgggcg gctacgacgc
     1081 gggctactac ggctacctgt ggtcggaggt catcggcgac gacctgttca gccggttcga
     1141 gcgcgaaggc gtcacctcgc cggcggtggg cgccgactac cgacgcgaga tcctcgagaa
     1201 aaacggcagc cggccggcgg cggagctggt ccacaacttc ctcggccgcg acttcacgcc
     1261 cgacgcgtgg ttccacctgc ggaacatgga ccagacctag ctccgcgcaa gcgcctcgag
     1321 tagctcggcg tgaagggcgc cgttggtggc cagcgccgac gggcccgtgc ggttctcgcc
     1381 gccgtccagg tcggtgaaac ggccgccggc ctccatcacg accagggcgg gcgcggcgag
     1441 gtcccagggc ttgacgccca gctcgaacat ggcgtcggcg gagccttggg ccacgagcat
     1501 gtagccccag aagtcaccga tcccccgatc ccgcgcggaa tggcgcaaca ccgcagccgc
     1561 cccctcgcca cggccgtcgt cccagaacgc accgagcccg gcatgaacga cgtgggcatc
     1621 ctccaatcgg ctgaccgatg acacgcggat cggccgctca acgccgctcc gcgaggtggt
     1681 cgctcccccg ccacggaacg cggaccagcg ctggcccagt gccggcgccg ccacgaccga
     1741 cgccagcagc tccccatctc gctccacggc gatgagcgtg gcccacacct ggatcccgcg
     1801 ggcgaagttc acggtcccgt cgatcggatc caccacccag cgcgtcccgt ccgcgccagc
     1861 cgggccgggc tgctccccga actcctcgcc cagcatggcg tgggtcgggt agcgctggcc
     1921 gatccgggtc cggatcaggg cctcgaccgc ggtatcggcc tcggtcaccg gcgttccgtc
     1981 gggcttgggg ctggccgcaa ccccggccag gaaggcgggc agggtgatcg catcggccgc
     2041 atcggccagg tcgagggcga acgcgcgcag ctcctcggcg tcagccgcca tgaggatcgg
     2101 agtccagccg gcgccgcagc tcctcgttca cgagcttggc atcggccttg ccgcccatcc
     2161 gggccatgac cttgcccacc aggtagccgt agacctgctg cttgccggac cggtactccc
     2221 ccaccgcgtc ggcctcggtc gccaggacgg ca
//
LOCUS       DASYGU010000023         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_518211, whole genome shotgun sequence.
ACCESSION   DASYGU010000023 DASYGU010000000
VERSION     DASYGU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518211"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VGO32_00575"
     CDS             complement(<1..1164)
                     /locus_tag="VGO32_00575"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV7199278.1"
                     /translation="MTSTETSPAVDTALAYLREHADEHIEGLNEFLRMESVSADPTKK
                     DEVRRTAQWIADAFTAIGFDNARLTETRSNPVVTADWLHAGPDKPTVLVYCHYDVQPP
                     DPLDEWVRPPFEPRYEDDILYARGSGDDKGQLYMHLRGAEAWLKGVGHLPLNLRVVFE
                     GDEEHLSEPVEEFIEAHPELLAADVCIVSDSDMHDDDGTPAITYGIRGISYWEIRVQG
                     PFQDIHSGTYGGGVDNPANVLVKMLASIVDGDGRITVPGFYDKVRDLTDADREAYAAV
                     PFDMDAFMEATGVTEPMAGETGWTLPERLSGRPTFDIDGIWGGYQGDGPKTIIPGFAA
                     AKISSRLVPDQDPREIEQMMIDHLRTIAPPTVTVTVRSLSNGAPAITPLDHPAV"
     gene            complement(1188..1460)
                     /locus_tag="VGO32_00580"
     CDS             complement(1188..1460)
                     /locus_tag="VGO32_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199279.1"
                     /translation="MLPFIWALMVFAVVGGFVMIVAYWLDIQDRSDLSAGAKIRWSAG
                     ILIFPVTVPLYAFFGGGHWPTLLKVAAFIPAAALTLFLLFVFGVLG"
     gene            complement(1470..2249)
                     /locus_tag="VGO32_00585"
     CDS             complement(1470..2249)
                     /locus_tag="VGO32_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7199280.1"
                     /translation="MTDRGAAGGREIGAHLLGVTVTWRYRLEWILVRILVRSLFRVRA
                     EGLGRWPAAPFQLSINHHSGWDPLLVLAISPLRPRITWFGPKEVNFSKGFRNRVMGFF
                     GGMIPFNPSKTNLGSSVRAVQRVFDSGGVLAIAAEGTLGFRESELLPLQDGATVFAAL
                     SGVPVLPGVIVGSSTLWLRKEITIRFGEPIAVSRGSSREARAEIAEQVRAGMAALLPE
                     REPVYPQRRPGSVFLTDLFNGPGERARWRKHMAEVAEPASD"
     gene            complement(2246..3472)
                     /locus_tag="VGO32_00590"
     CDS             complement(2246..3472)
                     /locus_tag="VGO32_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199281.1"
                     /translation="MYWLGILTIQFGLGSQIVPAMVEDFVGERQSGAGLAIVNAVAVI
                     ASIVVQPTVGVISDYTITRWGRRKPYIFIGGLLDMLFLYGIATSDTFVALVAFFFLIQ
                     VSSNFAQGPFQGYVPDLVPARQVGMASGLMGLMIVTGSIVGSGIAMFGLRTGGSLFAA
                     TMALGLVEIVAMLIVVTRVHEGPTGPKRTMSWPKVALSAWGTDILREKSVLWLLAVRL
                     LFLGAAAAATSLGLYYFQRTQGLPREEATQFVFFATVIVAGMSALASIPGARLSDRFG
                     RKAVIYGGMGLTAVGLVGWTLAPSPAAAVVIFVPAGIGIGAFLSVDWALMTDVIPKHT
                     SARYMGILNVGTALATPVFLIVGGLTMDAVGNAMGRAAGPVAAILVALGFLALSALAL
                     TQVNAQRRELTEPVAA"
     gene            3628..>4123
                     /gene="lgt"
                     /locus_tag="VGO32_00595"
     CDS             3628..>4123
                     /gene="lgt"
                     /locus_tag="VGO32_00595"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV7199282.1"
                     /translation="MFIDPVAFRIGPIAIHWYGIIFAVAAIAAAWLASREAKRRGMDT
                     ERVWSMLLVAAIGGIIGARLYHVIHEWDLIYRDNPALILQVWNGGLGIPGGVAGGMIA
                     LAIYTRVNHLPFLRWIDVGAPALLLAQAIGRFGNFVNQELYGPPTDLPWGIPIDAEHR
                     VPPWN"
BASE COUNT          709 a         1385 c         1389 g          640 t
ORIGIN      
        1 gacggccggg tggtcgagcg gggtgatggc cggcgcgccg ttcgagaggc tccggacggt
       61 gaccgtgacc gtcggcgggg cgatcgtccg caggtggtcg atcatcatct gctcgatctc
      121 gcgaggatcc tggtccggca cgagccggga gctgatcttg gccgcggcga agcccggaat
      181 gatcgtcttc ggcccatcgc cctggtagcc gccccagatg ccgtcgatgt cgaacgtcgg
      241 ccggccggac agacgctcgg gcagggtcca ccccgtctcg cccgccatcg gctcggtgac
      301 cccggttgcc tccatgaagg cgtccatgtc gaagggcacc gcggcgtacg cttcccggtc
      361 cgcatcggtc aggtcgcgca ccttgtcgta gaagccggga accgtgatcc ggccgtcccc
      421 gtccacgatc gaggccagca tcttcacgag aacgttcgcc gggttgtcga ccccgccccc
      481 gtacgtgccc gagtgaatgt cctggaacgg accctggact cggatctccc agtaggagat
      541 gcctcggatg ccgtaggtga tcgcgggtgt gccgtcgtcg tcgtgcatgt ccgagtcgga
      601 cacgatgcag acgtcggcgg ccaacagctc ggggtgggct tcgatgaact cctcgaccgg
      661 ttcgctcaga tgctcctcgt caccctcgaa cacgacccgc aggttgagcg gcaggtggcc
      721 gacgcccttc agccatgcct cggcgccgcg gaggtgcatg taaagctgcc ccttgtcgtc
      781 gccagacccg cgcgcgtaca ggatgtcgtc ctcgtaccgc ggctcgaacg gcggccggac
      841 ccactcgtcg aggggatcgg gcggctggac gtcgtagtgg cagtagacca ggacggtggg
      901 cttgtccggc ccggcatgga gccagtcggc ggtgaccacc gggttgctcc gggtctcggt
      961 cagccgggcg ttgtcgaacc cgatggcggt aaaggcgtcg gcaatccact gtgccgtgcg
     1021 ccggacctcg tccttctttg tcgggtcggc gctgaccgat tccatgcgca ggaactcgtt
     1081 caggccctcg atgtgctcat cggcgtgctc gcgcaggtag gccaatgcgg tgtcgaccgc
     1141 ggggctggtc tcagtcgatg tcatggtccg atggtatccg cgcagcctca gccgaggacg
     1201 ccgaagacga agagcaggaa cagggtcagg gccgcggccg ggatgaaggc cgcaactttc
     1261 aggagggtgg gccagtgccc tccgccgaag aacgcgtaca gcggcacggt caccgggaag
     1321 atgaggatgc ctgccgacca gcggatcttc gcgcctgcgc tcagatcgct tcgatcctgg
     1381 atatcgagcc agtaggccac gatcatcacg aacccgccga ccacggcgaa gaccatcagg
     1441 gcccagatga acgggagcac gcgcggatcc tagtcggagg ccggctcggc gacctcggcc
     1501 atgtgcttcc gccaccgcgc gcgctcccca ggaccattga acaggtcggt caggaacacg
     1561 ctcccggggc ggcgctgcgg atagaccggt tctcgctcag gcagcagcgc cgccatcccg
     1621 gcccggacct gttcggcgat ctccgcacgc gcctcgcggc tgctaccgcg cgagaccgcg
     1681 atcggctcac cgaatcggat tgtgatctcc tttcggagcc agagcgtgga actgcccacg
     1741 atgacgccgg gtagcacggg aacacccgac agcgccgcga agaccgtcgc gccgtcctgc
     1801 agtggcagga gctcggactc gcggaagccc agcgttccct cggccgcgat cgccagcacg
     1861 ccaccggagt cgaagacccg ctggacggct cggaccgatg agcccaggtt cgtcttcgac
     1921 ggattgaacg ggatcatgcc gccaaagaag cccatgaccc ggttccggaa gcccttggag
     1981 aagttcacct ccttggggcc gaaccaggtg atccgcgggc ggagcgggct gatcgccagg
     2041 acgagcaggg ggtcccagcc cgagtgatgg ttgatgctga gctggaaggg tgccgctggc
     2101 catcgcccga ggccctctgc tcggacccgg aacagtgaac ggaccaggat ccggaccagg
     2161 atccactcga gccgataccg ccacgtgacg gtcacgccga ggagatgggc gccgatctcc
     2221 cgcccgccgg ctgccccccg atccgtcacg cggcgactgg ctccgtcagt tcgcgacgct
     2281 gcgcgttgac ctgggtgagc gccagggcgg aaagggccag gaagccaagg gccaccagga
     2341 tggcggcgac cgggcctgct gccctgccca tcgcattccc gacggcgtcc atcgtgaggc
     2401 cgcccacgat caagaacacc ggtgtggcca gagctgttcc aacgttcagg atgcccatgt
     2461 agcgggccga cgtgtgcttg gggatgacat cggtcatcaa ggcccagtcc acggacagga
     2521 aggcgccgat gccgatgccg gccggcacga atatcacgac cgccgccgcg ggactcggag
     2581 cgagcgtcca gcccaccagg ccaacggcgg tgagtcccat gccgccatag atgactgctt
     2641 tgcgtccgaa tcggtcggag agccgcgcgc ccgggattga cgcgagcgcg gacatgcccg
     2701 ccacgatcac cgtggcgaag aagacgaact gggtcgcttc ctccctgggc aggccttgcg
     2761 tccgctggaa gtagtacaga cctagcgagg tcgccgccgc ggcggcgccc aggaacagga
     2821 ggcgcacggc aagcagccaa aggacgctct tctcgcgcag gatgtccgtg ccccatgccg
     2881 acagcgccac ttttggccag gacatcgtcc gcttcggtcc ggttggccct tcgtggactc
     2941 gggtgacgac gatgagcatg gccacgatct ccacaaggcc gagggccatc gtggcggcga
     3001 acagtgaacc gccggtccgc agtccgaaca tcgcaatccc cgacccgacg atcgagccgg
     3061 tgacgatcat cagacccatc agcccactgg ccatgcccac ctgtcgggcg gggacgaggt
     3121 ccgggacata cccctggaat ggtccctgcg cgaagttcga ggagacctgg atgagaaaga
     3181 agaacgcgac cagcgcgaca aaggtgtccg aggtcgcgat gccgtacagg aacagcatgt
     3241 ccagcaggcc gccgatgaag atgtacggct ttcggcgccc ccagcgggtg atcgtgtagt
     3301 cgctgatgac cccgaccgtg ggctgaacga cgatcgacgc gatcaccgcg accgcgttca
     3361 cgatggcgag gccggccccc gactgacgct cgcccacgaa gtcttcgacc atggccggca
     3421 cgatctggct tccgaggccg aactggatgg tcaggatccc gagccagtac accgacaggg
     3481 tcgccagcgt tcgcagggga agggccgctc ggtcgccgct gatggcggct ctcctctagg
     3541 gcgtgacctc gaacgatacg cgcaggcctg atggttggga aggtcgttcc gtcggatcga
     3601 aacagccggc gggtagaatc cgcgcgcatg ttcatcgatc cggtcgcctt ccgcatcggt
     3661 cccatcgcca tccactggta cggaatcatc tttgccgtcg ccgcgattgc ggccgcgtgg
     3721 ctggccagcc gcgaggcaaa gcggcgcggg atggacaccg agcgggtgtg gtccatgctc
     3781 ctcgtggccg ccatcggcgg gatcatcggg gcgcggctgt atcacgtcat ccacgaatgg
     3841 gacctgatct accgcgacaa cccggccctg atcctccagg tctggaacgg cgggctgggc
     3901 atccccggcg gcgtcgccgg cggcatgatc gcgctcgcga tctacacccg ggtcaaccac
     3961 cttcccttcc tgcgatggat cgatgtcggt gcgccggccc tgctcctggc ccaggccatc
     4021 ggtcgctttg gcaacttcgt caaccaggag ctgtacggac cgccaacgga cctgccgtgg
     4081 ggcatcccga ttgatgccga gcaccgggtc ccaccctgga aca
//
LOCUS       DASYGU010000024         6397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1032800, whole genome shotgun sequence.
ACCESSION   DASYGU010000024 DASYGU010000000
VERSION     DASYGU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032800"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1060
                     /locus_tag="VGO32_00600"
     CDS             116..1060
                     /locus_tag="VGO32_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7199283.1"
                     /translation="MSGQPLTAAAARRRWLLPAAFLLLGTIWGSSFVWIKIAIEELPP
                     ATLVAERLMLGAVAMLILLAFTRPARPTRGQVGHLAVMGFVNAGLPIFLISWGEQFID
                     SGTAAVLNSLVPIFSLLIAGLILRTERWSLFRVMGILIGFAGATVLASREFAFNPGPA
                     AIGGAVAVAAAALSYAIGASYGKYRIESTDRYVVAGGTLVFAALYVTIVAILADGGLT
                     LPTQPATIVGLLWLGLLGSFVAYVLYFFLLSHLGATVSTMTTYMFPVVGVTLGVLVLG
                     ESLDARLLLGTGLVVLGIAIVGLRYDSVVSLATRVARR"
     gene            1057..2301
                     /locus_tag="VGO32_00605"
     CDS             1057..2301
                     /locus_tag="VGO32_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199284.1"
                     /translation="MSGIEEPPDSVPTPSPSPAEETVWPARDFETTHSWDVKASGNRR
                     RPTAAEQAVPWLIGLVLALAGMLIVLLALIFSSNEGLLPAYGAASPSPTPGETATPRP
                     TPTPEPSAEPSPSVAATPIPEPTPAFPPLEIVFMQRTSEAGPIHLFSHDFAGSAALVP
                     QARDNRGVSTYDWAPSGAYGLAVVAGNPLALTPGNSARDLGDGFDAVTFAPDSDTAYA
                     IRATLAGANDRAELLRMDYSSGAVASLHTWVYPHPTTFQEDVVSEAQFADDGGFERVY
                     VLENGTVIVWILGAPQVYSFDPVSGVASTTDHMPFLWSPNGTLRIAVTESGSNSRFSV
                     RGIAGEERGALNVTGRVSHVRWSTASNQIVFTLTRATSSGGVAQDLYVWDLVTGHAGV
                     RLTQDLRSMGGEYRGSPDRWRL"
     gene            2298..2642
                     /locus_tag="VGO32_00610"
     CDS             2298..2642
                     /locus_tag="VGO32_00610"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEV7199285.1"
                     /translation="MIQEIVNPIAVELDETARVMRIRWDDGHLGVWGWTELRRACPCA
                     LCAGEGSLPGVVTLETMFPPEAVTMERVKWIGRYALAPIWADGHDTGLFTYNKLRLSC
                     ACDEHRAAGNED"
     gene            2670..3524
                     /locus_tag="VGO32_00615"
     CDS             2670..3524
                     /locus_tag="VGO32_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEV7199286.1"
                     /translation="MTFRKGIRLDPRQVRDLRGSRGGLAIGGGLGGVVLVVIVLLLGG
                     SPQDASTLTTVLSDLTVGTQQENDLTEECQTDVDANTREDCRIIGYVNSIQAYWGEAV
                     DGYQLAPTTFFTDAVSTGCGQATSAVGPFYCPVDATVYIDLGFFDLLESRFGAEGGPF
                     AEAYVIAHEYGHHVQNLIGVLQESGGAGAESGAVRTELQADCFAGVWAANAVDTGYLV
                     PLTTQQVAQALDAAAAVGDDRIQAQTQGQVNPETWTHGSSAQRQEWFQVGLESGDPNA
                     CNTFEADL"
     gene            3525..4373
                     /locus_tag="VGO32_00620"
     CDS             3525..4373
                     /locus_tag="VGO32_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV7199287.1"
                     /translation="MVSLASGFTLALGGGGARGYAHIGVAEALAERGLAPSRIIGTSM
                     GSVIGAGMAAGLSADQMVAMAARYDPWRMARRPARLAVFDHRPLMEQLVAEVGHPRIE
                     DLPIPYAAASYDLVTGRHELITSGSLADAVARSCAISVVFSPIEDGDVVWADAGIWEP
                     VPISLARRWSTDPVVAVRVIGSKPSWFARPPFLWSMQAGARLFGTQPRGERLGARRFV
                     GLLADRITDPVVRERADVLIVPNLIGVSWINFADVERPRRRGYEAARRVLDAVDAAEP
                     AEEAVV"
     gene            4370..5263
                     /locus_tag="VGO32_00625"
     CDS             4370..5263
                     /locus_tag="VGO32_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV7199288.1"
                     /translation="MSGGLLLVHAHPDDEVFSTGGAIAMALAEGRRVDLVVCTGGEEG
                     EVHDPDLDPTEAQPRLREIRAAELECALTALSAGAAAPDLLHLHMLGHRDSGMMGTPP
                     NDRPDAFWQADLATAAEPVVRLIREARPAVAIHYDETGGYGHPDHIQAHRIAVAALEA
                     AADASRSPEAGPSHRVGKRYETAFGSNKWLDLMTAMRERGIPLPWGYDEVLDAGEEAD
                     ERPGTVEPTVTTTVDVSAWLAAKRTAMACHRTQRQDFGWAIDLPDDLAGRAFGTERFV
                     LVERDGQPPPPNTVESSLFDR"
     gene            5303..>6397
                     /locus_tag="VGO32_00630"
     CDS             5303..>6397
                     /locus_tag="VGO32_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7199289.1"
                     /translation="MNCPACGTANEAGRKFCGECGTRLSAACPACGTANPPGMKFCGE
                     CGTALTDAPPAPAVAVEAGPPGPIAIGTERRLVTVLFGDLVGSTTLAEDRDPEETRNL
                     LSRYFETASEIIGRYGGTVEKFIGDAVMAVWGVPTAFEDDAERAVRAGLELIAEVYRI
                     LDAEQPLQMRVAVLTGEAAASIGAVGQGIVAGDLVNTASRLQGMAPAGTVLVGESTYR
                     ATRDAIAFEDVGDQVLKGKSSPVPAWRAVQVLGMRGGARRRSALEPPFVGRDEELRLV
                     KELFHATIRESKPRLVTIIGQAGIGKSRLGWEFEKYIDGVNQDFYWHAGRSPSYGEGI
                     SYWALAEMVRERAGIAETDDVPTARTKLTAC"
BASE COUNT          926 a         2245 c         2215 g         1011 t
ORIGIN      
        1 gttgcgatcg acctcgccca ggctggggtc gatctggcag gcagccaccc gaatcgtcat
       61 cgactcaatg ctctgctagg ctcccgcggt ccatggaccc gagtgtagcc gcgccatgag
      121 tggccagcca ctgaccgccg ctgcggccag acgccgctgg cttctgccgg cggcattcct
      181 attgctgggg acgatctggg ggtcgtcctt cgtctggatc aagatcgcga tcgaggagct
      241 gccgccagcc acgctcgtcg cggagcggtt gatgctcggc gcggtcgcga tgctgatcct
      301 gctcgcgttc acccgccccg cccggccgac ccgcggccag gtcggccatc tcgcggtcat
      361 ggggttcgtc aatgccggac tgccgatctt cctgatcagc tggggcgagc agttcatcga
      421 ctccggcacc gccgccgtcc tgaactcgct ggtcccgatc ttctcgctcc tcatcgccgg
      481 gctcatcctc cgcacggagc gctggtccct cttccgggtg atgggcatcc tgatcggctt
      541 tgccggcgcg acggtgctgg ccagccgtga gttcgccttc aaccccggac cggccgcgat
      601 cggtggggcc gtggccgtgg ccgcggcggc gctgagctat gccatcggcg cgagctacgg
      661 aaagtaccga atcgagagca cggaccgata tgtggtcgcc ggtggaacgc tcgtgttcgc
      721 cgcgctctac gtcacgatcg tggcgatcct cgcggacggc ggcctgaccc tgcccaccca
      781 acccgcgacg atcgtcggcc tcctgtggct cggcctcctc ggctcgttcg ttgcctacgt
      841 cctgtacttc ttcctcctga gccacctggg cgcgacggtg tcgacgatga cgacctacat
      901 gttcccggtg gtgggtgtca ccctgggcgt cctggtcctg ggcgagagcc tcgacgcgcg
      961 gctgctgctc gggacgggcc tcgtcgtgct cgggattgcg atcgttggcc ttcgatatga
     1021 ttcggtcgtg agcctcgcaa cccgcgtggc gcggcggtga gcgggatcga agagccaccg
     1081 gacagcgtcc ccaccccgtc cccgtctccg gcggaggaga cggtctggcc cgcgcgcgac
     1141 ttcgagacga cgcactcgtg ggacgtgaag gcatccggca accgtcgccg accgaccgcg
     1201 gccgagcagg ccgtcccgtg gctcatcggc ctcgttctgg ccctggccgg gatgctgatc
     1261 gtgctcctcg ctctaatctt ctccagcaac gagggtctcc tgccggccta cggcgcggcc
     1321 agcccgtccc ccacgccggg cgagacggcg actccgcgac cgacccccac ccccgagccc
     1381 agcgccgagc cctccccgtc ggtggccgcg acccccattc ccgagcccac gccggccttc
     1441 ccgccgctcg agatcgtgtt catgcagcga acctcggagg ccggaccgat ccacctgttc
     1501 agccacgact tcgccggatc ggccgccctc gtcccccagg cccgggacaa ccgcggcgtg
     1561 tcgacctacg actgggcacc cagtggggca tacggcctcg cagttgtcgc cggcaacccg
     1621 ctcgccctga ccccgggcaa ctcggcccgt gacctgggcg acggattcga cgccgtcacc
     1681 tttgcgcccg actccgatac ggcatacgcg atacgggcca ccctggccgg ggcgaatgac
     1741 cgggccgagc tgctgcggat ggactactcg agcggtgccg tggcctcgct gcacacctgg
     1801 gtgtatccgc atcccaccac cttccaggag gacgtcgtgt ccgaggccca gttcgccgat
     1861 gacggcggct tcgagcgcgt gtacgtcctc gagaacggga cggtcatcgt gtggatcctc
     1921 ggcgccccgc aggtctactc cttcgacccg gtctccggcg tggcaagcac gaccgaccac
     1981 atgccgttcc tgtggtcgcc gaacggcacg ctccgaatcg cggtcaccga gtctggatcc
     2041 aactctcggt tcagcgtgcg cgggatcgcg ggtgaagagc gcggtgccct gaacgtgacc
     2101 ggccgggtga gccacgtccg ctggtcaacg gccagcaacc agatcgtgtt caccctcacc
     2161 cgtgcgacat cgagcggtgg cgtggcgcag gacctgtacg tgtgggacct cgtgaccggg
     2221 catgccgggg ttcggctcac ccaggacctg cgctcgatgg gtggcgagta ccgcggctcg
     2281 ccggaccgct ggaggctgtg atccaggaaa tcgtcaatcc catcgcggtc gagctcgacg
     2341 agacggcgcg ggtcatgcgc atccgctggg acgacgggca ccttggcgtc tggggctgga
     2401 cggagctccg gcgcgcctgc ccgtgcgcgc tgtgcgcggg cgaggggtcg ctcccgggcg
     2461 tggtcaccct cgagacgatg tttccgcccg aagcggtgac catggagcgg gtgaagtgga
     2521 tcggccgata cgcgctggcc ccgatctggg ccgacggtca cgacaccggc ctgttcacct
     2581 acaacaagct ccggctgtca tgcgcctgcg atgagcatcg ggccgccggc aacgaggact
     2641 gaaggcctga accgccaccg tttccggaca tgacatttcg caagggcatt cgcctcgacc
     2701 cccggcaggt ccgcgacctg cgcggcagcc gcggcggcct cgccatcggt ggcggcctgg
     2761 gcggggtggt gctcgtcgtg atcgtgctcc tgctcggcgg ctcgccgcag gacgcatcga
     2821 ccctgaccac ggtcctgagc gacctgaccg tcgggaccca gcaggagaac gacctcaccg
     2881 aggaatgcca gaccgatgtc gacgccaata cccgcgagga ctgccggatc atcggctacg
     2941 tgaacagcat tcaggcgtac tggggcgagg ccgtcgacgg ctaccagctc gccccgacca
     3001 cgttcttcac cgacgcggtt tcgaccggct gcggccaggc aaccagcgcc gtcggaccct
     3061 tctattgccc ggttgatgcc acggtctata tcgacctcgg attcttcgac ctgctcgagt
     3121 cccggttcgg ggcggaggga ggtccattcg ccgaggccta cgtcatcgcc cacgagtacg
     3181 gccaccatgt ccagaacctg atcggcgttc tgcaggagag tggcggggcc ggcgcggaga
     3241 gcggagccgt tcggaccgag ctccaggccg actgcttcgc cggcgtgtgg gccgcgaacg
     3301 cggtcgatac cgggtacctc gtgccattga ccacccagca ggtggcccaa gcgctcgacg
     3361 cggcggcggc ggtgggggat gatcgcatcc aggcccagac ccagggtcag gtgaatccgg
     3421 agacatggac ccacgggtca tcggcccagc gccaggaatg gttccaggtc ggactggaga
     3481 gcggtgatcc caatgcctgc aatacgttcg aggcagacct gtagatggtg agcctggcgt
     3541 cggggttcac cttggcgctg ggcgggggcg gtgcgcgggg atacgcccac atcggggttg
     3601 ccgaggccct tgccgagcgt ggactggccc cgagccggat catcgggacg tccatgggtt
     3661 cggtgatcgg cgccggcatg gccgcaggcc tgagtgccga ccagatggtg gccatggccg
     3721 cccggtatga cccgtggcgg atggcccgac gtccggcccg cctggcggta ttcgaccatc
     3781 gcccgctcat ggagcagctg gtggcggagg tcggccaccc ccggatcgaa gacctgccga
     3841 tcccctatgc ggccgcgtcc tacgacctcg tcaccgggcg ccatgagctc atcacgtccg
     3901 gctcgctggc cgatgccgtc gcccgcagct gcgcgatctc ggtcgtgttc tcgccgatcg
     3961 aggacgggga cgtggtgtgg gccgatgccg ggatctggga gcccgtgccc atctcgctcg
     4021 cccgccgctg gtcgaccgat ccggttgtcg cggtccgggt catcggctcc aagccttcgt
     4081 ggtttgcccg tcccccgttc ctctggtcga tgcaggccgg tgcccggctg ttcgggaccc
     4141 agccccgcgg cgagcggctg ggtgcccgcc gattcgtcgg cctcctggcc gaccggatca
     4201 cggacccggt cgtccgcgaa cgggccgacg tcctgatcgt ccccaacctg atcggcgtca
     4261 gctggatcaa cttcgctgac gtcgagcggc cacgccggcg cggctacgag gctgcgcgcc
     4321 gcgtgctcga cgcggtcgac gcggcggagc cggccgaaga ggcggtcgtg tgagcggcgg
     4381 actgctcctc gtccacgccc accccgacga cgaggtcttc tccaccggcg gtgcgatcgc
     4441 catggccctg gccgaggggc ggcgggtgga cctcgtggtc tgcaccggcg gcgaggaggg
     4501 agaggtccac gatccggacc tcgacccgac cgaggcccag ccacgactcc gcgagatccg
     4561 ggcggccgag ctcgagtgcg cgctgaccgc cctcagcgcg ggcgccgccg cgccggacct
     4621 cctccacctc catatgctcg ggcaccgcga cagcgggatg atgggcaccc cgccgaacga
     4681 tcggccagac gcgttctggc aggcagacct cgcgaccgcc gccgaaccgg tcgtccgact
     4741 catccgcgag gcccgtccgg cggtcgcgat ccactatgac gagaccggcg gctacggcca
     4801 ccccgaccac atccaggccc atcggatcgc ggttgcggcg ctcgaggcgg ccgcggacgc
     4861 cagccggtcc cccgaagctg gtccttcgca ccgggtgggc aagcggtacg agaccgcatt
     4921 cggctcgaac aagtggctcg atctcatgac ggccatgcgg gagcgcggca tccccctgcc
     4981 gtggggctac gacgaggtcc tcgatgccgg cgaggaggcc gacgagcggc cgggaaccgt
     5041 tgagcccacg gtcacgacca ccgttgacgt gtcggcctgg ctggccgcca aacggaccgc
     5101 catggcctgc catcgaaccc agcgccagga cttcggctgg gccatcgacc ttcccgacga
     5161 cctggccggc cgggccttcg gcacggagcg cttcgtcctg gtcgaacggg acggtcagcc
     5221 gccaccgccg aatacggtgg aatcgagcct gttcgacagg tgatcggccc gctacagttg
     5281 gggttctgat gggtctcctg ccgtgaattg ccctgcctgc ggcaccgcca acgaggccgg
     5341 gcggaagttc tgtggcgagt gcggcacccg cctgagcgca gcatgtcccg cctgcgggac
     5401 cgcgaaccca ccgggaatga agttctgcgg cgagtgcggc accgccctga ccgatgcccc
     5461 cccggcaccg gcggtggccg tcgaagccgg tccgccgggg ccgatcgcga tcggcaccga
     5521 acgccgcctc gtgaccgtgc tcttcggcga tctggtgggt tcgaccacgc tggccgagga
     5581 tcgcgatccg gaggagacgc ggaacctcct gagccggtac ttcgaaaccg cctcggagat
     5641 catcggtcgg tatggcggca cggtcgagaa attcatcggt gacgccgtga tggcggtgtg
     5701 gggcgtgccg actgcgttcg aagatgacgc ggaacgggcg gtccgcgccg gccttgagct
     5761 cattgcggag gtctaccgca tcctggacgc cgagcagccg ctccagatgc gcgtcgcggt
     5821 gctcaccggt gaagcggcgg cctcgatcgg ggcggtcggc cagggcatcg tcgctgggga
     5881 cctggtgaac acggcaagcc ggctgcaggg aatggcgccc gcgggcacgg tcctcgtcgg
     5941 ggagtcgacc tatcgcgcga ctcgcgacgc catcgccttc gaggatgtcg gcgaccaggt
     6001 gctgaaggga aagtccagtc ccgtgccggc gtggcgagcg gtccaggtgc ttggcatgcg
     6061 tggcggcgca cgccgccggt ccgcgctcga gcccccgttc gtggggcggg acgaggagct
     6121 ccggctggtc aaggagctct tccacgcgac catccgcgag tcgaagccgc ggctggtcac
     6181 gatcatcggc caggcgggca tcggaaagag ccggctgggc tgggagttcg agaagtacat
     6241 cgatggcgtg aaccaggact tctactggca cgccggtcga tcaccgtcct atggcgaagg
     6301 catcagctac tgggccctgg ccgagatggt ccgtgagcga gcggggatcg cggagaccga
     6361 cgacgtaccg accgcgcgaa cgaagctgac ggcgtgc
//
LOCUS       DASYGU010000025         4381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_673076, whole genome shotgun sequence.
ACCESSION   DASYGU010000025 DASYGU010000000
VERSION     DASYGU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673076"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VGO32_00635"
     CDS             complement(<1..1838)
                     /locus_tag="VGO32_00635"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV7199290.1"
                     /translation="MDLTRLRAAPAAVVQRITRTGEPSSMPGPELADLIAEARRHRPT
                     INVALIERAHEVATAAHEGQLRASGEPYITHPTAVAFELATLQMDAETLAAGLLHDVP
                     EDTGYPLADIEKRFGREVSRLVDGVTKLSKFSSARSTEEQQAENIRKMFMAMAEDARV
                     VIIKLADRLHNMRTLQYLAPEKQERIARQTMEIYAPLAHRLGMWQIKWELEDLAFKVL
                     DPDTYARLGGLLADSRKARETFINRSITILRKELAAVGIEADISGRPKHIYSIVKKME
                     RKGAEFHEIYDLHAIRVLVEDVKDCYGALGVVHAIWRPIPGQFDDYIAMPKANLYQSL
                     HTAVIGPDAKPLEVQIRTGLMHEVAEAGIAAHWRYKEGSRADRRYDEKLAWVRQLMEW
                     QREVSDATEFVEGLKLDVFQDQVFVFTPKGDVKDLPAGATPLDFAYRIHTDVGHRTIG
                     AKVNNRLVPLDYRLRNGDIVEIVTTKASHGPSRDWLAIVQTNQAREKIRAWFKRQQRD
                     ENIAQGRELLDRELRRLAHETLATIDAARLTEIATQLHFRELDDFYAAVGYGAVSPAS
                     VVSRLGIHDDVAISLPEVAPPAAPSTAGVRVKGVGDLLVRFANCCSP"
     gene            complement(1886..2974)
                     /locus_tag="VGO32_00640"
     CDS             complement(1886..2974)
                     /locus_tag="VGO32_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV7199291.1"
                     /translation="MNRPPADRQALAAWPVTDAATWDAFVTTAEHRAFPQLWAWGDLR
                     LEAGWRPIRIAVGSDRDRPRAGVQLLLRRVPLSGWSLAYAPRGPIGTLDDPLVREALV
                     AALWSLAEDERIGRVRADPETTDHDPFGASLLQPPWQAAPKVQPPTTMLIDLTTPEDA
                     LWSALGRKHRQYVSKAGREGVTVQRLDPDANDSATAAALAEFDRIYRHTGDRAGFALR
                     VPAYYRRVWDTFSVDGRARLSFAVRDGERVATLFHLVCGDRVAEVYGGMTEAGAASRA
                     NYLLKWEAIRAMQAEGLRTYDLWGLATGGIRRFKEGFGGTEVTWVGARDLPLHRTGNA
                     ILALALAGHGARRRLESIGRRSATPTVD"
     gene            complement(2955..>4381)
                     /locus_tag="VGO32_00645"
     CDS             complement(2955..>4381)
                     /locus_tag="VGO32_00645"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV7199292.1"
                     /translation="VTALTAQSAEATPGTAFFAIPGRVHDGWTFIPEAVANGAVAIIA
                     ERETPGLVVPQILVADARHAVADAADAWFGRPSEQLRVLGITGTDGKSTTAFLLLDVL
                     LAAGRRPGMVGTIDTRIGDTQTASTSRTTTPEALELQALLATMVEAGNDSVVIEATSH
                     GLAQSRVRNCRFRVGVVTTITSEHLEFHGTLEAYREAKAILVEESPVAVLNADDENFA
                     FLRGKAAGSVISFGIESEADVRASEIDLGPHGSRFHVSGPRWEGPVTLRLPARFNVGN
                     ALAALAVAEAEGLDPAMAAEALASVSGVPGRMERIDLGQPFGVIVDYAHTADSLAKVL
                     RELRPLTAGRLIAVFGSAGDRDRTKRAPMGRVAAELADLVVITDEDPRGEAPWDVNEE
                     IAVGARTAGAQDRETMWIIDDRRAAIAHALRLAEAGDMVLLAGKGHEPSIIYGDDRRW
                     WDEREVARQELRAIGFQTDEPAAG"
BASE COUNT          605 a         1509 c         1567 g          700 t
ORIGIN      
        1 gggctgcagc agttcgcgaa ccggacgagg aggtccccca cgcccttgac ccggaccccg
       61 gctgtgctcg gcgcagccgg cggcgcaact tccggcaggg agatggcaac gtcgtcgtgg
      121 atcccgagcc ggctgacgac cgacgccggg ctgaccgccc cgtagccgac cgcggcatag
      181 aagtcgtcca gctcgcggaa atggagctgg gtcgcgatct cggtcagccg ggcggcatcg
      241 atcgtggcca atgtctcgtg ggccagacgg cggagctccc ggtcgagcag ctcgcgtccc
      301 tgggcgatgt tctcgtcgcg ctgctggcgc ttgaaccagg cccggatctt ctcgcgcgcc
      361 tggttggtct ggacgatcgc cagccagtcg cgtgacggtc cgtgcgaggc cttggtggtc
      421 acgatttcga cgatgtcccc gttgcgcagc cggtagtcga gcgggacgag ccgattgttg
      481 accttggcgc cgatcgtgcg atgcccgaca tcggtgtgga tccgatacgc gaagtcgagc
      541 ggagtcgcgc cggcgggcag gtccttcacg tcgcccttgg gcgtgaacac gaacacctga
      601 tcctggaaga cgtcgagctt gaggccctcc acgaactcgg tcgcgtcgga cacctcgcgc
      661 tgccactcca tgagctggcg gacccaggcc agcttctcgt cgtagcggcg gtcggcgcgg
      721 ctgccttcct tgtagcgcca gtgggcggcg atcccggcct cggccacctc gtgcatgagc
      781 ccggtccgga tctggacctc gagtggcttc gcgtcggggc cgatcaccgc ggtgtggagc
      841 gactggtaca ggttggcctt gggcatcgcg atgtagtcgt cgaactggcc cgggatcggt
      901 cgccagatgg cgtgcacgac ccccagcgcg ccgtagcagt ccttcacgtc ctcgacgagg
      961 acgcggatgg cgtggaggtc gtagatctcg tggaactcgg cccccttgcg ctccatcttc
     1021 ttgacgatgc tgtagatgtg cttgggtcga ccgctgatat cggcctcaat gccgaccgcc
     1081 gcgagctcct tgcggagaat cgtgatcgag cggttgatga acgtctcccg ggccttccgg
     1141 ctgtcggcca gcaggccacc gaggcgggca taggtgtccg ggtcgaggac cttgaaggcg
     1201 aggtcctcga gctcccactt gatctgccac atgcccagcc gatgcgcgag cggggcgtag
     1261 atctccatcg tctgccgcgc gatccgctcc tgcttctcgg gcgcgaggta ctggagggtc
     1321 cgcatgttgt ggagccgatc ggccagcttg atgatcacga cccgcgcgtc ctcggccatg
     1381 gccatgaaca tctttcggat gttctcggcc tgctgctcct cggtcgatcg ggcactgctg
     1441 aacttggaca gcttggtgac cccgtcgacg aggcgcgata cctcccggcc gaaccgcttc
     1501 tcgatgtcgg ccagcgggta gccggtgtcc tccggcacat cgtggagcag gcccgccgcc
     1561 agggtctcgg cgtccatctg gagcgtggcc agctcgaagg cgaccgcggt cgggtgggtg
     1621 atgtacggct cgccggaggc gcgcagctgg ccctcgtgcg ccgcggtggc cacctcgtgg
     1681 gcgcgttcga tgagggcgac gttgatggtc ggccgatggc gccgcgcctc ggcgatgagg
     1741 tcggccagct ccgggcctgg catgctcgac ggctcgccgg tccgcgtgat gcgctggacg
     1801 acggcggccg gcgcggcgcg gaggcgtgtc agatccatgg tcgtttcgat cgtagcacgg
     1861 gctcctgagc ctgccccgcg cggcgctagt cgaccgtggg cgtggccgat cggcggccga
     1921 tgctctcgag acggcggcgc gccccgtgcc cggccagggc gagggccagg atcgcgttgc
     1981 cggtccggtg gagcgggaga tcgcgggcgc cgacccacgt gacctcggtc ccgccgaagc
     2041 cctccttgaa gcgacggatg ccaccggtcg ccaggcccca caggtcgtag gtacgcagcc
     2101 cctcggcctg catcgctcgg atcgcctccc atttcaggag gtagttggcc cgagaagcgg
     2161 cgcccgcctc ggtcatccca ccgtagacct cggccacgcg atcgccacag accaggtgga
     2221 acagggtcgc cacccgctct ccgtcgcgga cggcgaagga caggcgcgcc cgaccgtcga
     2281 ccgagaaggt gtcccaaacc cgacggtagt aggcgggcac gcggagcgcg aacccggccc
     2341 gatcgcccgt gtggcggtag atccggtcga actcggccaa cgccgccgcg gtggccgagt
     2401 cgttggcatc tgggtcgagt cgctggacgg tgaccccttc tcggccggcc ttcgacacgt
     2461 actgccggtg tttgcggccg agcgcggacc acagcgcgtc ctcgggcgtg gtgaggtcga
     2521 tcagcatggt cgttggcggc tggaccttgg gcgcggcctg ccacggcggt tggaggagcg
     2581 aggcgccgaa cggatcatgg tcggtggtct cgggatcggc acggacccgc ccgatccgct
     2641 cgtcctcggc cagggaccag agcgcggcga cgagtgcctc tcggacgagc gggtcatcga
     2701 gcgtgccgat cggcccgcgc ggcgcgtagg ccagcgacca gccgctcagt ggcactcggc
     2761 ggagcaggag ctggacgccg gctcggggcc gatcgcggtc cgagccgacg gcgatccgaa
     2821 tcggccgcca tcccgcctcg agacggaggt ccccccacgc ccatagttgg gggaaggcgc
     2881 ggtgctcggc ggtcgtcacg aaggcgtccc acgtggcggc atcggtcacc ggccaggccg
     2941 ccagggcctg ccggtcagcc ggcggccggt tcatcggtct ggaatccgat ggcgcgcagc
     3001 tcttggcggg cgacctcgcg ctcgtcccac catcggcggt cgtcgccgta gatgatgctc
     3061 ggctcgtggc ccttgccggc caggaggacc atgtccccgg cctcggccag ccgcagcgcg
     3121 tgggcaatcg ccgcccgccg atcgtcgatg atccacatcg tctcgcggtc ctgggcgccc
     3181 gccgtccgtg cgcccacggc gatctcctcg ttcacgtccc acggcgcctc tccgcgtgga
     3241 tcctcgtcgg tgatgaccac caggtcggcc agctcggccg cgacccggcc catcggcgcg
     3301 cgcttcgtgc ggtcgcgatc ccccgccgac ccgaagaccg cgatcagccg ccccgcggtg
     3361 agcggccgga gctcgcgcag caccttggcc agcgaatccg cggtatgggc gtagtccacg
     3421 atcacgccga acggctggcc gaggtcgatg cgctccatgc gaccgggcac tccgctgacc
     3481 gaagcgagcg cctcggcggc catcgccgga tccaggccct cggcctcggc caccgccagc
     3541 gcggccaacg cgttgcccac gttgaatcgg gcggggaggc gcagcgtcac ggggccttcc
     3601 catcgcggtc ccgagacgtg aaagcgggag ccgtggggtc cgagatcgat ctcggacgcc
     3661 cggacgtcgg cctcggactc gatcccgaag ctgatcaccg agcctgctgc cttaccgcga
     3721 aggaacgcga agttttcgtc atcggcattg agcacggcca ccggagactc ctcgaccagg
     3781 atggccttcg cctcgcggta ggcctcgagc gtgccgtgga attcgaggtg ctcgctggtg
     3841 atggtggtca cgactccgac ccggaaccgg cagttgcgca cccgcgactg ggcgaggcca
     3901 tgggaggtgg cttcgatcac gaccgagtca ttgccggcct cgaccatggt cgcaagcagc
     3961 gcctggagct cgagcgcttc gggcgtcgtc gtccggctgg tcgatgcggt ctgggtgtcg
     4021 ccgatccggg tgtcaatggt cccgaccatg ccgggccgcc gaccggctgc caacaggacg
     4081 tcgagcagga ggaacgcggt cgtcgacttg ccgtcggtgc cggtgatgcc cagcacccgg
     4141 agctgctcgg atggccgccc gaaccaggcg tccgccgcgt cggccaccgc gtggcgggcg
     4201 tcggcaacca ggatctgggg cacgaccagg ccgggtgtct cgcgttcggc aatgatcgcg
     4261 actgccccgt tggccacggc ttcggggatg aacgtccacc cgtcgtggac ccggccggga
     4321 atcgcgaaga atgccgtccc gggcgtcgcc tcggcgctct gggcagtgag cgcggtgacc
     4381 t
//
LOCUS       DASYGU010000026         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_261513, whole genome shotgun sequence.
ACCESSION   DASYGU010000026 DASYGU010000000
VERSION     DASYGU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261513"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VGO32_00650"
     CDS             complement(<1..702)
                     /locus_tag="VGO32_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199293.1"
                     /translation="MTDPSPGTVPSASGSVVTFEHVTKRYGDGPPAVTDLSLEIDAGE
                     ICVLVGPSGCGKTTTMKMVNRLVEPTSGRITIDGQDVASLPPVELRRRIGYVIQHVGL
                     FPHMTVGDNVAVVPRLLGWAAPRVAERVEELLDLVGLDPGAYRARYPAALSGGERQRV
                     GVARAMAADPTVLLMDEPFGAVDPIRRERLQNEFLRLQARLRKTIVFVTHDVDEAIKM
                     ADRIVILQVGGIVAQY"
     gene            complement(699..2711)
                     /locus_tag="VGO32_00655"
     CDS             complement(699..2711)
                     /locus_tag="VGO32_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199294.1"
                     /translation="MNRKAAFVVAAGIGIGLVAAWVWPPLAVLTVWPLLVIVPGWAIV
                     AWATRGGARLASTGRLGLAIVLSVAVSAHLVWWLSTAAGGYGREVVFVAAALLAVPIP
                     LAASRRSGAWQVGARDRWRAAVRALRRRPGAFGLALVALGFVGLVLARGLWQVNADGV
                     DVGGSNWSDLGVHLSIAQSVNAGNFPPDVPFFAGEPLVYHWFADFHAAILAEAAGLFA
                     IPVFIVQSALLAGALALVVHGLALTLIRGRLARRAAWVAVFLVVFGGGFGWIRLVGDL
                     GNELGAAWDLLMTHAYDNQWLSDWPYFSIPSVMTTGLLVHRATTVGLPLMAGAVLMAV
                     VGIPSARRRATGFVDRPRLVVAAGIAAALLAPFHFFFFPVVPMLVLGWVVIGRRFGRN
                     VLRLAVLFAAPFLLAVPFALGALGQATSSGWLEPVIGWPTAPLKDGPLAVLFFYVTNL
                     GIPFVLAVAAIALRRTPSRAFLALWIGLLFLIPNVVQVSYVSFDMNKYFQAMWIAVGI
                     AAGALIARWPAPVIALVLALSAISPMLSSVHHAFSQNGLMSADALAAAEWIAVQTPER
                     SVFVTDSWIISPTDPAGRLRLTSFGPYIANLGYDPGPREEQIAEIRCGGDPIRAAEVM
                     ADLGATYVFPAGGPGCERPTDFGSSALFEEAYANGSVTIYRLSSAP"
     gene            2748..3308
                     /gene="folE"
                     /locus_tag="VGO32_00660"
     CDS             2748..3308
                     /gene="folE"
                     /locus_tag="VGO32_00660"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV7199295.1"
                     /translation="MSFEDAVRTILTEIGEDPERQGLRETPERVRRMFEQITDGYRVD
                     PDSILEGAQFDVDYDEMVVVRDIELYSLCEHHLVPFFGHAHVGYLPRGKVIGLSKIPR
                     VIDMFAHRLQVQERMTSEIAEFLMTRLEPKGVGVVLQTTHLCTAMRGVRRPGATMVTS
                     AMLGTFRRDPKTRSEFLAFIGSSFET"
     gene            3359..3793
                     /locus_tag="VGO32_00665"
     CDS             3359..3793
                     /locus_tag="VGO32_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV7199296.1"
                     /translation="MTGDASMTPEGSARPIRVLLAKPGLDGHDRGAKVLARGLRDEGF
                     EVIYTGLRQTPEMVADAAEQEDVDVVGLSILSGAHMTLLPRIVELVRAKGMDDVLIAA
                     GGIIPDADISKLKAAGVAEVFGPGTTIGQVATYFRDHARPRG"
BASE COUNT          674 a         1320 c         1347 g          486 t
ORIGIN      
        1 gtactgggcc acgatcccgc cgacctgcag gatgacgatc cggtcggcca tcttgatcgc
       61 ctcgtccacg tcatgggtca cgaacacgat cgtcttgcgg agccgggcct ggaggcggag
      121 gaactcgttc tggagccgct cccgtcggat ggggtcgacc gcaccgaacg gctcatccat
      181 gagcaggacg gtgggatcgg ccgccatcgc ccgggccaca cccacccgct gacgctcgcc
      241 gccggagagc gctgccggat atcgcgcgcg gtatgcgccc ggatcgaggc ccacgaggtc
      301 gagcagctcc tcgacccgct cggcgacacg gggcgcggcc cagcccagca gacgaggcac
      361 gaccgccacg ttgtcgccga ccgtcatgtg cgggaacagg ccgacgtgct ggatgacgta
      421 gccgatccga cggcggagct cgaccggggg caggctggcg acatcctggc cgtcaatcgt
      481 gattcgtccc gaggttggct ccaccagccg gttgaccatc ttcatggttg tcgtcttccc
      541 gcatccggaa ggtccgacca ggacgcagat ctcgcccgca tcgatctcga gcgacaggtc
      601 ggtgaccgcg ggtggcccat cgccgtatcg cttggtgacg tgttcgaagg tcaccaccga
      661 gccgctcgcg gacggcaccg tcccgggcga cggatcggtc atggcgccga tgataggcgg
      721 tagatcgtga cgctcccgtt ggcgtaggcc tcctcgaaca gcgcgctgga cccgaagtcg
      781 gtcgggcgtt cgcaaccggg gccgccggcg gggaacacgt acgtcgcgcc caggtcggcc
      841 ataacctccg ccgcgcggat tgggtcgccg ccgcatcgga tctcggcgat ctgctcctcg
      901 cgcgggccag ggtcgtagcc gagattggcg atgtacgggc caaaggaggt gagccgcaga
      961 cggccggcgg ggtcggtggg ggagatgatc cagctgtcgg tcacgaacac cgaccgctcc
     1021 ggggtttgca cggcgatcca ctcggcagcg gcgagcgcat cggcgctcat gaggccgttc
     1081 tgcgagaacg cgtggtggac ggagctcagc atcggcgaga tggcgctgag ggccagcacg
     1141 agggcgatta ccggggcggg ccagcgggcg atcagcgcgc cggcagcgat cccgacggcg
     1201 atccacatcg cctggaaata cttgttcatg tcgaacgaga catagctgac ctggacgacg
     1261 ttcgggatga ggaagaggag accgatccac agcgcgagga aggcccggga cggggtccgc
     1321 cggagggcaa tggctgccac ggcgaggacg aacggaatgc ccaggttggt gacgtagaag
     1381 aacaggaccg ccagcggccc gtccttcaat ggcgcggtgg gccagccgat gaccggctcc
     1441 agccagcccg agctcgtggc ctgccccagg gcgcccagcg cgaacgggac ggcgagcagg
     1501 aacggcgccg caaacagcac ggcaaggcgg agcacgttcc ggccgaaccg ccggccgatg
     1561 accacccaac ccagcacgag catcgggacg accgggaaga agaagaagtg gaatggggcc
     1621 aggagcgccg ccgcgatgcc ggccgcgacc accaaccgtg gtcgatcgac gaagccggtg
     1681 gctcgacggc gcgcgcttgg gatcccgacc acggccatca gcacggctcc cgccatcagc
     1741 ggcagcccga cggtcgttgc ccggtggacg aggagcccgg tggtcatgac cgatgggatg
     1801 gagaagtacg gccaatcgga cagccattgg ttgtcgtagg catgggtcat gagcaggtcc
     1861 caggcggcgc cgagctcgtt gcccaggtcc cccaccaggc gaatccaccc aaacccgccc
     1921 ccgaacacga ccaggaagac ggcgacccag gccgcgcgtc gcgccagccg gccacggatc
     1981 agggtcagcg ccaggccatg gaccaccagc gcgagggcgc cggcgagcag cgccgactgg
     2041 acgatgaaga cggggatcgc gaagaggccc gcggcctcgg ccaggatggc cgcgtggaaa
     2101 tcggcaaacc agtgatacac gagtggctcg ccggcgaaga acgggacgtc gggcgggaag
     2161 ttccccgcat tcaccgattg ggcgatcgac aggtggacac caaggtcgct ccagttggat
     2221 ccccccacgt ccaccccgtc ggcattgacc tgccacaggc cgcgggcgag gaccagtccg
     2281 acgaacccga gcgcgaccag cgccagcccg aacgcgcccg gccgccgacg gagcgcccga
     2341 acggcggctc gccaccgatc gcgagcgccc acctgccatg cgccggacct ccgcgatgcc
     2401 gcgaggggaa tggggacggc gagcagggct gccgcgacga acacgacctc ccgcccgtag
     2461 ccgccggccg cggtggacag ccaccagacg agatgggcgg agaccgccac cgagagcacg
     2521 atcgccaggc cgagccgtcc ggtcgacgcc aggcgcgcgc cgccgcgggt cgcccacgcg
     2581 acgatcgccc agcccggaac gatgaccagc agtggccaca ccgtgagcac ggcgagtggc
     2641 ggccagaccc acgccgccac cagcccgatg ccgatgcccg ccgccactac gaatgccgcc
     2701 ttccggttca cggagaggac gataccgggc tgctagcatc ggtggccatg agcttcgagg
     2761 acgcggtccg gacgatcctg accgagatcg gggaggaccc cgagcgccag ggcctgcgcg
     2821 agaccccgga gcgggtacgg cggatgttcg agcagatcac cgatggctat cgggtcgacc
     2881 ccgactccat cctcgagggc gcccagttcg acgtcgacta cgacgagatg gtggtcgtcc
     2941 gcgacatcga gctgtactcg ctgtgcgagc accacctcgt gccgttcttc ggccacgccc
     3001 acgtgggata cctgcctcgg ggcaaggtca tcggcctatc caagattccg cgagtgatcg
     3061 acatgttcgc ccaccggctc caggtccagg agcggatgac ctcggagatt gccgagttcc
     3121 tgatgacccg gctcgaaccc aagggggtcg gggtcgtgct tcagaccacg cacctgtgca
     3181 ccgccatgcg cggcgtccgc cgaccggggg cgacgatggt cacctccgcc atgctgggca
     3241 ccttccgtcg agacccgaag acccggtccg agttcctggc cttcatcggc tcgtcgttcg
     3301 agacctgacc gatggcgccc ggagccggcc agaccgccgg actagaatcg cggcgctcat
     3361 gactggtgac gcctcgatga cgccggaggg ctccgcgcga ccgattcgcg tcctgctggc
     3421 caagcccggg ctggacggcc acgaccgcgg ggccaaggtc cttgcccgcg gcctgcgcga
     3481 cgaggggttc gaggtgatct acaccggcct ccgccaaacg cccgagatgg tggccgacgc
     3541 cgccgagcag gaggacgtgg acgtcgtggg cctctccatc ctgtccggcg cccacatgac
     3601 cctcctgccc cggatcgtgg agctggtccg ggccaagggc atggatgacg tcctgatcgc
     3661 ggccggcggg atcatccctg acgccgatat ctcgaagctc aaggccgccg gggtggccga
     3721 ggtgttcgga ccggggacca ccatcggcca ggtcgcgacc tacttccggg accacgcccg
     3781 cccgcgtggc tgacctcgcg gaacggctgc gggaggcgca ggagggg
//
LOCUS       DASYGU010000027         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1393410, whole genome shotgun sequence.
ACCESSION   DASYGU010000027 DASYGU010000000
VERSION     DASYGU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393410"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VGO32_00670"
     CDS             complement(<1..1617)
                     /locus_tag="VGO32_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEV7199297.1"
                     /translation="MLAKYLIAAGIEVANPNQVSGTIELDEPVTSLAVAPQRTYLGWP
                     ASVVFTSDGTDTIEARNATTGDPIAAWDAPDRVTSLVFDQDTSRLLVGLRSDGTIATF
                     DLTTFLTQQGERAPPPASVGIDTGMDGVLQIVSSNSETVLAVRGPGEVAVFERVTGVE
                     LSRRDIDVDAISYVAAVGGDQPVSARVIVLDLDAGTVQTLDATTMSTESSQRPSSAPL
                     GALMTSGQGTSQLTWVPIGPLPATDEHPAVSGGLAIYRGGTIIPDATVPLPGPGLALG
                     WDPVANLVYVAGESAVWVIEPHGDNRSGYGVYDEAPFAGTPAALGFDVSDTSQSDDHG
                     QLIVAAADDDGAALVRVDISQNAFAWRLAATAFGALLAGLVYLLTALLFRRRSIAILA
                     GVFVAIDLMSFAMSRIAMNDIFVALFIMAGYALFWPIWGGRWKRSAWWVLPLVGVMIG
                     LAAASKWVGWYALIGLWFLILVRSQLGRFVVIGATGFAAVAVGAGAPWPFTLIVFAIL
                     GLGLAVAWVRPVRLESAELWAVPATALVVGA"
BASE COUNT          268 a          571 c          584 g          222 t
ORIGIN      
        1 ggccccgacc acgagggcgg tggccggcac ggcccacagc tcggccgact ccaggcggac
       61 gggccggacc caggcgacgg cgagccccag acccaggatc gcgaacacga tgagcgtgaa
      121 tggccacggt gcgccagcgc cgacggcgac cgccgcaaag ccggtggcgc cgatgacgac
      181 gaaccggccg agctgcgatc ggacgaggat gaggaaccac agaccgatga gcgcgtacca
      241 gcccacccac ttcgaggcgg cggccaggcc gatcatgact ccgacgaggg gcaggaccca
      301 ccaggcagat cgtttccatc ggccccccca gatgggccag aagagggcgt agccggccat
      361 gatgaacagc gcgacgaaga tgtcgttcat cgcgatccgg ctcatcgcga agctcatgag
      421 gtcgatggcc acgaataccc cggccaggat cgcgatcgag cgacgccgga agagcagggc
      481 ggtcagcagg tagaccagcc cggccaggag cgccccgaag gcggtggccg ccagccgcca
      541 ggcaaacgcg ttctggctga tgtcgacccg gaccagggcc gccccgtcgt catccgcggc
      601 ggcaacgatc agctggccgt gatcgtcgct ctgggaagtg tcgctgacgt cgaatcccag
      661 cgccgccggg gtgccggcga agggagcctc gtcgtagacc ccgtatccgg atcggttgtc
      721 gccgtgcggc tcgatgaccc acaccgccga ctcgccggcg acatacacga gattggcaac
      781 cggatcccac ccgagggcca ggcccggacc gggaagcggg acggtcgcgt cggggatgat
      841 cgtcccgccg cggtagattg ccaggccgcc gctgaccgcg ggatgctcgt cggtcgccgg
      901 caacgggccg atcgggaccc aggtcagctg gctcgtcccc tgcccgctgg tcatcagcgc
      961 gccgagtggt gccgaggacg gccgttggct ggactcggtg ctcatcgtgg tcgcgtccag
     1021 cgtctggacc gtgccggcgt cgaggtcgag cacgatcacc cgcgcactga ccggctggtc
     1081 cccgccgacg gcggcgacgt acgagatcgc atccacgtcg atgtcccggc gggacagctc
     1141 gacgccggtc acccgctcga agaccgccac ctcgcccggc ccgcgtacgg cgagcacggt
     1201 ttcgctgttg gaggacacga tctggagcac gccgtccatc ccggtgtcga tgccgacact
     1261 cgccggtggc ggcgctcgct cgccctgctg ggtaaggaac gtggtgaggt cgaacgtggc
     1321 gatcgtgccg tctgagcgaa ggccgaccag caggcggctg gtgtcctggt cgaagacgag
     1381 gctcgtgacc ctgtccggcg catcccaggc cgcgatcggg tcgccggtag tcgcgttcct
     1441 ggcctcgatc gtgtcggtcc cgtccgatgt gaagacgacc gacgcgggcc agcccaggta
     1501 cgttcgctgc ggcgccacgg ccaggctggt cacgggctcg tccagctcga tcgtgccgct
     1561 cacctggttc gggttcgcca cctcgatccc ggccgcgatg aggtacttgg ccagcatcgg
     1621 gtgggtccac tcgtaggggt cccgg
//
LOCUS       DASYGU010000028         7939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_158859, whole genome shotgun sequence.
ACCESSION   DASYGU010000028 DASYGU010000000
VERSION     DASYGU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158859"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VGO32_00675"
     CDS             complement(<1..538)
                     /locus_tag="VGO32_00675"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEV7199298.1"
                     /translation="MPWPAVAAAVIGLVGVGAERLASTWPEDEAQHRPASWRTAVMGL
                     VSAAAAYAVVAVSHLPAWAVLVHLVMLAFMIGLVATDLEQRRLPHLLLDPLIVVAVGF
                     AFVNPALETSLAIAGAAAAVVFLGILAFIVRGGLAVGDLYLVVPIGLTLGWPLIWSGL
                     FTAALLVGAVSLFLVVTGR"
     gene            complement(541..2022)
                     /locus_tag="VGO32_00680"
     CDS             complement(541..2022)
                     /locus_tag="VGO32_00680"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEV7199299.1"
                     /translation="MRLRAEATDPASVEANVLAVPIYKEDEPFGEDLANLDATAGGVI
                     RQAIDFGRFNILEDYFALIDGGDLPVEHILIVNGIRRGRGPWRARRMAGVATRALQGK
                     GAARMALWLRDGEDADGFAAAAVGAVAGTYRPMDLYGRTRDTDAMKRIVEEVVLLGAD
                     QAELDRASIMADGVAWCRDLSNRASNDLYPEKMAEEVRALEADGCTVEVLHVPEMRQL
                     GMNALLGVGQGAEHPPVLIAVKLPGWAAGGDRKLAIVGKGVCFDSGGISLKPADKMDE
                     MKHDKSGAAAVLSAARTLARLNPNAPVMAVAPMVENMPGGRAQRPGDVVKAMNGKTIE
                     ITNTDAEGRLILADALHWAETQGATHLVDVATLTGVAAMAFGELVSAYFAKPGDWGDQ
                     VKAAADATGEWLWEMPLATEYRTQLDTPYADIVNSGSRDGSLIKSAVFIHEFVTRPWV
                     HLDVAGTAWMAADKATFPKGAVGVAMTTLVRLGLDFAAGEQVA"
     gene            complement(2101..2973)
                     /locus_tag="VGO32_00685"
     CDS             complement(2101..2973)
                     /locus_tag="VGO32_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199300.1"
                     /translation="MKRIVAALAVLVAVCLLFGAAPADTGTADPVRVAALQRRAVTLA
                     AEADATLLEAEALMRTGSRDGGRAQAAVLSGDEDPATFADAAALSFESAAGPIDAARE
                     PLVALGWTLLALDPETAPPTLGLTGADLLAIGAQWRATALPLSALAELRRDAEATLTS
                     LGEALAALDDDDPEAALVALAEAEASLALVRAFDTDVSTLPFWIETVDALLTATGDIA
                     RAAQAGDAAALAEAQAAYDAAADDAGRADQALTIALGEATTQVTSAATAATAEAIHAV
                     EATRAALAGVSILP"
     gene            2994..4055
                     /gene="add"
                     /locus_tag="VGO32_00690"
     CDS             2994..4055
                     /gene="add"
                     /locus_tag="VGO32_00690"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEV7199301.1"
                     /translation="MATDHTDLVETLRRLPKAELHQHLDGAVRPETAVELAVEAGIDL
                     TLEQARQRLVAPERCRDQADLLSYFELPLQLLQTPAALRRVATELVQDMATDGVTYGE
                     IRWAPRLHLEEGLGVGDVLEAVAGGVAEARTAGGASAPLIGLVVTAMRSHPPGANVEL
                     AHRAASFGPPVVGFDLAGPEAEYPAPPHAAAFRAAAAAGLGLTAHAGETPGTERIWEV
                     LEFGISRVAHGAPAVDDERLLETLRARSITLDLCPTSNVQAGIVPSLAAHPITRLHRA
                     GVSVTLSTDDRTVSRTTLSGEMADVAIATGMTAEELAEIAINGFRRGFSSPLELAEPR
                     AEAARAWHAWTATRNPLPA"
     gene            complement(4072..4935)
                     /locus_tag="VGO32_00695"
     CDS             complement(4072..4935)
                     /locus_tag="VGO32_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV7199302.1"
                     /translation="MSDHGDPPSTARPPLRLVILDMDGVLYRGDRAIPGAANLVGLLH
                     EAGVAVRYATNNSMFTRDQYAERLAAMDIPAAANEIVTSTSATIEHLRRHQPDVRAVL
                     AVGAAGMVTELDAAGYSVVPIAAAASAAADAQPFDAVLVGLDPDFDEGRLAVAAAAVR
                     AGARFVATNADARYPTPSGFRPGAGAMVAAIAEASGVAPLVIGKPEPAMFAAILEAAG
                     VEPAAALVIGDNPDSDIAGAHRSGIRSVLVLTGVTDADQAVGLTGDRRPDHVVADPAA
                     AWDLLEGWLAP"
     gene            5096..6256
                     /locus_tag="VGO32_00700"
     CDS             5096..6256
                     /locus_tag="VGO32_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7199303.1"
                     /translation="MRKRNYAFSAMFVSLALVLGACSGGQISTGAGLCREVGDAVAAE
                     PSTPESTGTSDLVIGLVTDVGTLDDAGFNQYSWEGALQGAAIIGAPEPEAIVSTDSSQ
                     YEDNINSFVDAGANLIVTVGFAAGAATQAAATENPDIHFVGVDQFQVNTDFGGEWEPL
                     PNYESLIFSEQQAGYLAGILAGSISESGEVAALGGSGTIPPVVNYMRGFENGVHSVNA
                     DATVHLKYVSNDLTTAFNDPTTGKTFADEFLAVNENVDLLFAVAGKTGNGMLDSVVEH
                     GIYGFGVDVDQWVSLPDAAICIVTSAEKHLTLGVSESITSFAAGDVRSNNVFYGADNN
                     GIGLAPYYQFSDLVTDDIQAAIDDAFDAMASGDLNPCGDPSTCYAGEEDPGT"
     gene            6379..>7939
                     /locus_tag="VGO32_00705"
     CDS             6379..>7939
                     /locus_tag="VGO32_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199304.1"
                     /translation="MTDATNSATTNRGQDAPVLEMRGITKQYPGVTACDGISLDLRAG
                     EIHALLGENGAGKTTLMNILFGLAVPGSGDILLNGEVTEIASPHDAIARGISMVHQHF
                     MLVPVLSVAENILLGEEPMKRKIFLDRAGSRARIRELGERFGWEIDPDRKVGQLSVGW
                     QQRVEILKALYRDTKVLILDEPTAVLTPQETREIFDVLRRLRDEGYAIVFISHKLYEV
                     LEIADRITVIRRGKVVGSRKPSETNEDDLAELMVGREVQLTVDRGTSHPTEPVLTVEA
                     LHVNDDRGREVVHDVSLTIRKGEIFGVAGVAGNGQDELVEALTGLRKPSSGSVQLAGQ
                     DVTGAGPRRLHQAGVGFVPGDRHRFGLVLSFPLTDNLVLNDYYHPPYSRGGVRNEDAI
                     TRDAEDAIARYDVRTPSATVTASTLSGGNQQKVIVAREFEGDLKLLVLDQPTRGLDVG
                     SIEFIHRQTISRRDTGVGILLVSAELDEILELSDRIAVMYRGRLVATVDGATAEKERI
                     GLMMATGEVPDQ"
BASE COUNT         1245 a         2811 c         2752 g         1131 t
ORIGIN      
        1 cccggccggt gacgaccagg aacaggctca ccgcgccgac caacagggcg gcggtgaaca
       61 ggccgctcca gatcagcggc caccccaggg tgagaccgat gggcaccacg aggtacaggt
      121 caccgaccgc gagcccaccg cggacgatga acgcgaggat gcccaggaag accaccgcgg
      181 cggcggcccc ggcaatggcc aggctggtct cgagggcggg gttcacgaag gcgaacccga
      241 ccgcgaccac gatcagcggg tcgaggagca ggtgcgggag gcggcgttgc tcgaggtcgg
      301 tggccaccag gccgatcatg aacgcgagca tcacgaggtg gacgagtacc gcccacgcgg
      361 gcaggtggga cacggccacc acggcatacg ccgcggccgc cgagacaagc cccatcacgg
      421 ccgtccgcca gctggcaggt cggtgctgcg cctcgtcttc cggccaggtg ctggccaggc
      481 gctccgcgcc cacaccgacg agaccgatta cggccgccgc gacggcgggc cagggcacgc
      541 tcaggcgacc tgttccccgg ccgcgaagtc caggcccagg cgaacgagtg tggtcatcgc
      601 gactcccacc gcgcccttcg ggaaggtggc cttgtcggcg gccatccagg ccgtgcccgc
      661 cacatccaga tggacccagg ggcgggtgac gaattcatgg atgaagaccg cgctcttgat
      721 cagcgagcca tcccgcgagc cgctgttgac gatgtccgcg tacggcgtgt cgagctgggt
      781 ccggtactcg gtcgcgagcg gcatctccca cagccactca ccggttgcat ccgccgccgc
      841 cttgacctgg tcgccccagt cccccggctt cgcgaaatag gcgctcacca gctcgccgaa
      901 ggccatggcc gcgaccccgg tgagggtggc gacgtccacc aggtgggtgg cgccctgggt
      961 ttcggcccag tggagcgcat cggccaggat cagccggcct tcggcatcgg tgttcgtgat
     1021 ctcgatggtt ttcccgttca tggctttgac cacgtcaccg gggcgctggg cgcggccgcc
     1081 gggcatgttc tcgaccatgg gggcgaccgc catcaccggg gcgttggggt tgagccgggc
     1141 cagcgttcgg gccgcggaca ggaccgcggc ggcgccggac ttgtcgtgct tcatctcgtc
     1201 catcttgtcc gccggcttga ggctgatgcc accggagtcg aagcacacac ccttgcccac
     1261 gatggccagc ttccgatcac cgcccgctgc ccagccgggc aacttcaccg cgatgaggac
     1321 cggtggatgc tccgcgccct ggcccacgcc cagcagcgcg ttcatgccca gctgacgcat
     1381 ctcgggcacg tggaggacct ccaccgtgca accgtcggct tccagggcgc gaacctcctc
     1441 ggccatcttc tcgggataca ggtcattcga ggcgcggttt gacaggtcgc ggcaccacgc
     1501 gacgccgtcg gccatgatcg aggcccggtc gagttccgcc tggtccgctc cgagcaggac
     1561 gacctcctcg actatccgct tcatggcatc ggtgtcgcgg gtccgaccgt agagatccat
     1621 cggccggtac gtccccgcca ccgcgcccac cgccgcggcc gcgaaaccat cggcgtcctc
     1681 tccgtcgcgc aaccagagcg ccatccgtgc cgcacccttg ccctgcagcg cacgggtcgc
     1741 gaccccggcc attcgccggg cgcgccacgg ccctcggccg cgacgaatgc cgttgacgat
     1801 gaggatgtgc tcgaccggca ggtctccgcc atcgatcagc gcgaaatagt cctccaggat
     1861 gttgaaacgg ccaaagtcga tggcctgccg gatgacaccg cccgcggtcg cgtccaggtt
     1921 ggcgaggtcc tcgccgaacg gctcgtcctc cttgtagatc gggaccgcca ggacgttcgc
     1981 ctcgaccgat gccgggtccg ttgcctccgc gcgaagccgc atctgctctc ctcggtgcgt
     2041 gtctgtcggt ggccaaggtg cgcgtcgcgg ggccatgctc gggggcgtcg cgcctgaagg
     2101 tcaggggagt atagacacgc ccgccagcgc cgcccgcgtc gcctccaccg cgtggatcgc
     2161 ctcggccgtt gccgccgtcg ccgcggacgt gacctgggtc gtggcctcgc ccagcgcgat
     2221 ggtcagcgcc tggtccgccc gtcccgcgtc atccgccgcc gcgtcgtacg ccgcctgggc
     2281 ctcggccagc gccgccgcgt cgcccgcctg ggcagcccgg gcgatgtcgc cggtggcggt
     2341 cagaagcgca tccacggtct cgatccagaa cggcagggtg gacacgtcgg tgtcgaaggc
     2401 ccgcacgagc gccaacgagg cttccgcctc ggccagcgcc accagggcgg cctccgggtc
     2461 atcgtcatcc agggccgcga gggcctcacc cagcgaggtg agcgtcgcct ccgcgtcgcg
     2521 ccggagctcc gccagggcgg agaggggtaa cgcggtcgca cgccactggg cgccgattgc
     2581 gagcagatcg gcgccggtca ggcccagggt cggcggtgcg gtttccggat cgagcgcgag
     2641 gagcgtccag cccagggcca cgagcggctc gcgcgcggcg tcgatcgggc cggccgccga
     2701 ctcgaacgac agggcggcgg catcggcaaa cgtggccgga tcctcgtcgc cggacaggac
     2761 cgcggcctgc gcccgaccgc catcgcggct gccggtccgc atcagcgcct cggcctcgag
     2821 gagggtcgca tcggcctccg cggccagcgt caccgcgcgg cgctgaaggg cggccacccg
     2881 gaccgggtcc gcggtcccag tgtcagctgg cgcggccccg aacaggaggc agacggcgac
     2941 cagcacagcc agggcggcca cgattcgttt cacgcggctc ctatcatgcc ccgatggcca
     3001 ccgaccacac cgacctggtc gagaccctgc gtcggctgcc caaagccgag ctgcatcagc
     3061 atctcgacgg cgccgtgcgc ccggagacgg cggtggagct cgccgtcgaa gcgggcatcg
     3121 acctcactct cgagcaggca cggcagcgac tggtggctcc agagcgctgc cgcgatcagg
     3181 cggatctgct cagctacttc gagctcccgc tccagctcct ccagacaccc gccgccctgc
     3241 gccgggttgc caccgagctg gtccaggaca tggccaccga tggggtgacc tacggcgaga
     3301 tccgatgggc gccgcggctg cacctggagg aggggctggg agtgggcgac gtgctcgagg
     3361 cggtggccgg cggcgtggcc gaagcgcgga ccgcgggtgg ggcgtcggcg ccgctgatcg
     3421 ggctggtggt gaccgccatg cgctcccatc cgcccggcgc gaacgtggag ctcgcccatc
     3481 gcgccgcgag cttcggtccg ccggtggtcg gctttgacct cgccggcccg gaagcggagt
     3541 acccggcccc gccgcatgcc gccgcgttcc gggcggcggc cgccgccggc ttgggactca
     3601 ccgcgcacgc cggggagaca cccggcacgg aacggatctg ggaggtcctc gagttcggca
     3661 tcagccgggt ggcccacggg gcgccggccg tcgacgacga gcggctgctg gagacgctcc
     3721 gcgcccggag catcaccctg gacctgtgcc ccaccagcaa cgtccaggcc ggcatcgtgc
     3781 cgtcgctcgc cgcacatccc atcacccggc tccaccgggc gggggtgagc gtgaccctgt
     3841 ccaccgacga ccggacggtg agccggacca ccctcagcgg ggagatggcg gacgtggcga
     3901 tcgccactgg catgaccgcc gaggagctgg cggagatcgc gatcaacggc ttccggcgag
     3961 ggttcagctc tccgctcgag ctggcggagc cgcgggccga ggcggcacgc gcctggcacg
     4021 cctggaccgc gacccggaac ccactccccg cctgacgcgt cggcggcccg gtcagggagc
     4081 cagccagccc tccagcaggt cccacgccgc cgccggatcg gcgacgacat ggtccgggcg
     4141 acggtcgccg gtcaatccga ccgcctggtc cgcatcggtc accccggtca ggaccaggac
     4201 gctccggatc ccggaccgat gcgccccggc gatgtcggag tcggggttgt cgccgatgac
     4261 cagcgccgcg gcgggctcca cgccggccgc ctccaggatc gcggcgaaca tggccggctc
     4321 gggcttgccg atgaccagcg gcgcaacgcc gctggcctcg gcgatggcgg ccaccatggc
     4381 gccggctccg ggacggaagc cgctgggcgt ggggtagcgg gcgtccgcgt tggtggccac
     4441 gaaccgggca ccggcgcgta cggcagccgc ggcgacggca agccggccct cgtcaaagtc
     4501 gggatccagg ccgacgagca ccgcgtcgaa cggctgggca tcggccgcgg cgctggccgc
     4561 cgcagcgatg ggcacgaccg agtagcccgc ggcgtcgagc tcggtgacca tcccggccgc
     4621 tccgaccgcc agcacggccc gaacgtctgg ctgatgccgc cgcaggtgct cgatggtggc
     4681 cgaggtggag gtcacgatct cgttcgcggc ggccgggatg tccatcgcgg ccaggcgctc
     4741 ggcgtactgg tcgcgggtga acatcgagtt gttcgtcgcg tagcggaccg cgacgccggc
     4801 ttcgtgcagc aggcccacga ggttggccgc gccgggtatc gcccggtcgc cgcgatacag
     4861 gaccccgtcc atgtccagga tgaccagccg aaggggcggc cgagcggtcg agggcgggtc
     4921 gccgtggtca gacatgggcg acacggtaga cgacagcgga aaacccttga gggcgttcgg
     4981 gcgggaagat atgatgccgc cgactcgcgt gggctctgcc ctcatgggcc ccgagcgcag
     5041 ccgcgccatc gccgcggcga gatccatccc gttacggagg gcggaaggag aacgcatgcg
     5101 gaagcgcaat tacgcattca gcgcgatgtt cgtgtcgctt gcgctggtat tgggcgcctg
     5161 ttctggcggt cagatcagca cgggggcggg cctctgccga gaggtagggg acgccgtcgc
     5221 ggctgagccg tcgactccgg agtccaccgg caccagcgac ctggtcatcg gcctggtcac
     5281 cgacgtcggc acgctggacg acgcgggctt caaccagtac agctgggaag gcgcgctgca
     5341 gggcgccgcg atcatcggtg ctcccgagcc ggaggccatc gtctccacgg actcgtcgca
     5401 gtacgaagac aacatcaact cgttcgtgga cgccggggca aacctgatcg tcacggttgg
     5461 cttcgcagct ggcgctgcga ctcaggccgc ggcgacggag aacccggaca tccacttcgt
     5521 gggggtcgac cagttccagg tcaacaccga cttcggtggc gagtgggagc cactcccgaa
     5581 ctatgagtcg ctcatcttca gcgagcagca ggctggctac ctggccggca tcctggccgg
     5641 ttcgatttcg gagagcggcg aagtggccgc cctcggcgga tcgggcacca tcccgccggt
     5701 cgtgaactac atgcgcggct tcgagaacgg cgtgcactcg gtgaacgccg acgccaccgt
     5761 gcacctgaag tacgtcagca acgacctgac caccgccttc aacgacccga ccacgggcaa
     5821 gaccttcgcc gacgagttcc tcgcggtgaa cgagaacgtc gacctcctgt ttgcggtcgc
     5881 cggcaagacc ggcaatggca tgctggattc ggtcgtcgag catggaatct acgggttcgg
     5941 cgtggacgtc gaccagtggg tctccttgcc cgatgccgcc atctgcatcg tgaccagtgc
     6001 cgagaagcac ctgaccctgg gcgtcagcga gtccatcacg tcgttcgcgg cgggcgacgt
     6061 ccgcagcaac aacgtgttct acggtgcgga caacaacggc atcggcctgg cgccgtacta
     6121 ccagttctcg gacctcgtga ctgacgacat ccaggcggcg atcgacgacg cgttcgacgc
     6181 gatggcgagc ggcgacctga atccgtgcgg cgatccgtcc acgtgctacg ccggtgaaga
     6241 ggaccccgga acctagtagc tctcgccgag taggacccta gcccaccgag cgggccgatc
     6301 cggactggat cggcccgctc acgcatatcc cccgatcttc acgtttgccc gtaaccaggg
     6361 tgatgcatcg ccgctgacat gaccgacgcc accaacagcg ccaccacgaa ccgcggccag
     6421 gacgctcccg tgctcgagat gcgcggcatc accaagcagt acccgggcgt cacggcttgc
     6481 gacggcatca gcctcgacct gcgagcgggt gaaatccatg cgctgctcgg cgagaatggc
     6541 gcggggaaga ccaccctgat gaacatcctc ttcgggctgg ccgtccccgg ctccggtgac
     6601 atcctcctga atggggaggt cacggagatc gcgagcccgc acgatgccat tgctcgcggg
     6661 atcagcatgg tgcaccagca cttcatgttg gtcccagtct tgtcggtggc cgagaacatc
     6721 ctcctcggcg aggagccaat gaagcggaag atcttcctcg accgagccgg ttctcgggcg
     6781 cggattcgcg agctcggcga gcggttcggc tgggagatcg acccggaccg caaagtcggc
     6841 cagctgtccg ttgggtggca gcagcgggtg gagatcctca aggcgctcta ccgcgacacc
     6901 aaggtgctca tcctcgacga gcccaccgcg gtgcttaccc cgcaggagac gcgggagatc
     6961 ttcgatgtcc ttcgccgtct ccgcgacgag gggtacgcca tcgtcttcat ctcccacaag
     7021 ctgtatgagg tcctggagat cgccgatcgg attacggtca tccggcgggg caaggtggtg
     7081 ggcagccgca agccgtccga gaccaacgaa gatgatctgg ctgagctgat ggtcggccgt
     7141 gaggtccagc tcaccgtcga ccgcgggacg agccacccga ccgagccggt cctcaccgtc
     7201 gaggccctcc acgtcaacga cgatcgtggg cgcgaggtcg tgcacgacgt gagcctcacc
     7261 attcggaaag gcgagatctt cggggtggcc ggcgtggcgg gtaatggaca ggatgagctg
     7321 gtcgaggccc tcacgggcct gcgcaagccg tcatccggat cggtccagct ggcgggccag
     7381 gacgtaacgg gcgccggacc gcggcgcctg caccaggcgg gcgtgggctt cgtccccggc
     7441 gaccggcatc gattcgggct ggtgctcagc ttcccgctca ccgacaacct ggtcctgaac
     7501 gactactacc accctccgta cagccgaggc ggcgtccgca acgaggacgc catcacgcgg
     7561 gatgcggagg acgcgatcgc ccgctacgac gtgcggaccc catccgccac cgtcaccgca
     7621 tccaccctct caggcgggaa ccagcagaag gtcatcgtgg ctcgcgaatt tgagggcgac
     7681 ctcaagctgc tcgtcctcga ccagccgacc cgtggcctcg atgtcggttc gatcgagttc
     7741 atccaccgcc agaccatctc tcgccgtgat accggagtcg gcatcctcct cgtctccgcc
     7801 gagctggacg agatcctcga gctgtccgac cggatcgcgg tgatgtaccg cggtcggctc
     7861 gtcgcgacgg tcgatggggc gaccgcggag aaggaacgga tcggcctgat gatggcgacc
     7921 ggcgaggtcc ccgaccagg
//
LOCUS       DASYGU010000029         7090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_158913, whole genome shotgun sequence.
ACCESSION   DASYGU010000029 DASYGU010000000
VERSION     DASYGU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158913"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VGO32_00710"
     CDS             <1..1361
                     /locus_tag="VGO32_00710"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199305.1"
                     /translation="QDDRIHGVLSTYAGSGFKVAYLSEDEQTALRDQVIVPYFQAEQV
                     GDGLLAAMGVIADEVTPGSTAALNFYRQANAVIGIPGGILVLLFTLGTAFVVWRRQGD
                     DPDVIDSPSVLMAGPPADMTPPLATVLTEGRATGNAIRTLMVELAGTGLIRFRNLERV
                     KDVKRDDDPDPLTDPAIEIVGVTVNREQPTGVLAEAFRELVTLGGSTGELRRESLWAV
                     NETLEPTKRRLEEEGVRLGWLTRRPTPEITKWIGIGVLEAIAGGGAIFLGYAIPMSGL
                     TLVGGALVIGGLATAAFGTQMSKRTENGAYVDAMLKAFRRTLQKTLEQARSMAEVAQE
                     PTVRVLADTPDKAVVWGVALGLHQQVGEVLAREMADPQMRAAAQSSGSYYPMWIGSSF
                     AGDSGGGGGGSLFSGSGTPDVGGMLGTIGSVGSAPASSSSGGGGFGGGGGGGGGGGSS
                     GF"
     gene            1611..2264
                     /locus_tag="VGO32_00715"
     CDS             1611..2264
                     /locus_tag="VGO32_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199306.1"
                     /translation="MSLRLDPRHLATLRNLAAEAGVRPGELVTIWVTERLDAERRGAS
                     SPSDVGPVLESLNERIETLSRRISALEGGKTSQADDGPADSTPEPAPARRRGRPPKAA
                     TAAAAAAANGDAGTAVPTDAPTKKGKRKKQAAGKTKHARGKGKNPAVPLHEEIAAVLA
                     ERGPMTAGDLAKAVSERGVYAPPRSAKALDATAVSVRVSNPRYRSRFTRADGKIGLA"
     gene            complement(2261..2464)
                     /locus_tag="VGO32_00720"
     CDS             complement(2261..2464)
                     /locus_tag="VGO32_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEV7199307.1"
                     /translation="MSVARVTELSCTSPDSFEDAVRQGVERAQRTLRGVRGAWVKEQR
                     VNINSDGSIEFQANILVTFVLED"
     gene            complement(2501..3427)
                     /locus_tag="VGO32_00725"
     CDS             complement(2501..3427)
                     /locus_tag="VGO32_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="phosphotriesterase homology protein; PhP; YhfV;
                     member of a family of proteins related to
                     phosphotriesterase (PTE); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEV7199308.1"
                     /translation="MTVSGPIPPESVGFALLHEHTRVSLWHIPGRFDYWELAADELTI
                     ADELRDYRRRGGRTLVDLTVPGIGRDPERLRRLASRTGVQIVMGCGWYRGPYYPAEAL
                     IDRRSVDSLADELVSEFEHGVGETGIQPGIIGEIGTDKPWISALEERVHRAAARAAIR
                     TGMTVTTHGVQSAVGEAQLDIFLSEGVDPGRVVIGHADSWPDLDHYLAILDRGANLQF
                     DFLGHRFDPVDEQREPRIVELIVELLERGYGAQILLSQDVCHDMQLKVNGGFGYTYLQ
                     QHFLPALRTAAVGEGELERIMVENPRRILTLP"
     gene            3635..4228
                     /locus_tag="VGO32_00730"
     CDS             3635..4228
                     /locus_tag="VGO32_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199309.1"
                     /translation="MNQRPTGVTILAVLAFIGGAFGILGALALLGLGVAFAGVAGGGL
                     SFIFGIVFLALSILYLWIGWGFWNLKRTAWSLGLVVFGAGIVISLIEMLLGYASITNL
                     IVTIVINGIVIYYLLTPGVKAAFGVEGDLQSAVMSGLPMGGTASTSAPAATPPAAPAP
                     PAAPPPAEPPASTWSGSGESSGGSTPSAGTDDSDPNA"
     gene            4327..5595
                     /locus_tag="VGO32_00735"
     CDS             4327..5595
                     /locus_tag="VGO32_00735"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV7199310.1"
                     /translation="MNEPEPSYSGTQEWAAAQAEVDDVAARLKLDDGMHRVLRVAKRE
                     LEVHFPIQFDDGRVDAVTGWRVHHNINRGPSTGGVRYTADLTLDLVRANAMWNTWKAA
                     IVEIPYGGAFGGVVVDPKQLSAHEVEGLTRRYTTEISPLIGPSQDIPMPDVNTDEQTM
                     AWMMDTYSMQRGYTIRGVVTGKPPAIGGTRGQRDAVSLGALRAIQAVARTQGLSVDGA
                     RVVMQGFGRVGTILAEMMAGAGARIVAIADDRHAIRNPSGIEVGPAVAHMAAHDSAEG
                     LPGGEPMDRADVFAEPCDILVSAGLQGQISATNAPRIEARIVAEAANGATSPEADLAL
                     YDNGVTVIPDLLCTAGGFVVGYLEWVQDLQSFFWSDAEVTAELDRVMNDAAAAVMAAA
                     DRHHVDLRTAALMVGIGRAAEATTLRGLYP"
     gene            5602..6966
                     /locus_tag="VGO32_00740"
     CDS             5602..6966
                     /locus_tag="VGO32_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7199311.1"
                     /translation="MAEQHDDRTASHDQPERYGGDGLDLDRDRLTLRFESFELERTFQ
                     VDLGLRLRLQHRVGLFLATGLWITAGLMLIVIYGVDPLEIALAISVPMAFVIGGLAII
                     GRFETWDAQQVVNGIINLVGGMSMIFISTRVADVPEALGTVLILNAIFAFSVVRMGFV
                     IGLAAELPVLIWVAALAWIGTYPEIGWFTVFLLASGLGVAAYGAYMLESSARARFLQR
                     HDLARQGEALAREKAKSDRLLHTMLPASIADRLRDEPRVIADAFPDATVVFSDIVGFT
                     PIGSDMAPVDLVTVLNELFGRFDELAARHGLEKIKTIGDGYMAVAGVPLAMDDHADRA
                     VRFGLAIHRATNDLGAELSMPLQLRVGIHSGPLVAGVIGRDKLAYDLWGDTVNVASRM
                     ESQGMPGRVQISEATALRLNEEVSLEPRGTIEVRGRGPMRVLLVNEPDQTDGAVPVAE
                     TARA"
BASE COUNT         1107 a         2433 c         2475 g         1075 t
ORIGIN      
        1 tccaggacga ccgcatccac ggcgtcctga gcacgtacgc gggcagcggg ttcaaggtcg
       61 cctacctgtc cgaggacgag cagacggctt tgcgcgacca ggtgatcgtt ccgtacttcc
      121 aggccgagca ggtgggagac ggcctgctgg cggcgatggg cgtcatcgcc gacgaagtca
      181 cgcccggatc gaccgcggcc ctcaacttct atcggcaggc aaacgccgtc atcggcattc
      241 cgggcgggat cctcgtcctg ctcttcacgc tggggaccgc gttcgtcgtg tggcggcgcc
      301 agggagacga tccggatgtc atcgactcgc catcggtcct gatggccggg cccccggccg
      361 acatgacccc gccgctggcc acggtcctga ccgagggccg agcgacgggc aacgcgatcc
      421 gcacactcat ggtcgagctc gccgggaccg ggctcatccg gttccgcaac ctcgagcggg
      481 tgaaggacgt gaagcgcgac gacgatccgg atccgctgac cgacccggcc atcgagatcg
      541 tcggcgttac cgtgaatcgc gagcaaccga cgggcgtcct ggccgaggcc ttccgcgagc
      601 tggtcaccct cggtgggtcg accggtgagc tgcgacgcga atcgctgtgg gcggtcaacg
      661 agacactcga gccgaccaag cgtcggctcg aggaggaggg cgtccggctc gggtggctca
      721 cccgccggcc gacacccgag atcacgaaat ggatcggcat cggcgtcctg gaggcaatcg
      781 cgggtggcgg ggcgatcttc ctcggttacg cgatcccgat gagcggcctc accctcgtcg
      841 ggggcgccct cgtcatcggc gggctggcca ccgctgcgtt cgggacccag atgtcgaagc
      901 ggaccgagaa tggggcctac gtcgacgcca tgctcaaggc gttccggcgc accctccaga
      961 agacgctcga acaggctcgc tccatggcgg aggtggcgca ggagccgact gttcgcgtcc
     1021 tcgccgacac gccggacaag gcggtcgtgt ggggagtcgc cctcgggctc catcagcagg
     1081 tcggcgaggt cctcgcgcgc gagatggcgg acccccagat gcgagccgcc gcccagtcgt
     1141 cgggctccta ctacccgatg tggatcgggt cgtcattcgc cggcgattcc ggcggtggcg
     1201 gcgggggcag cctcttctcg ggatcgggca cgccggacgt gggtgggatg ctcgggacca
     1261 ttggcagcgt gggctccgcg cccgcgtcgt catccagcgg cggaggcggc ttcggtggcg
     1321 ggggcggcgg aggcggcgga ggcgggagca gcggcttcta aatcattaca aactttgcgc
     1381 acatttcaaa tgttgggtat cgtggggcca tggcatacga ccgcgaccga cgaccacctc
     1441 gcccccgccg cccggatgac cggcgaccgg gtggccctga ccgataccgc cccgccgacc
     1501 gcgggccaga ccgtggcccc gaccgcggag gcgaccgtcc ttttcgcccc cgtccgccct
     1561 atcagtccgg cccgcggcca ccgcgcagcg gtccgcccga tgagggcggg atgtccctcc
     1621 gcctcgatcc ccgccacctg gcgaccctgc gcaacctggc cgccgaggcc ggcgttcggc
     1681 ccggtgagct ggtcacgatc tgggtcaccg agcggctcga cgcggagcga cgcggagcgt
     1741 cctccccgag cgacgtcggc ccggtcctcg agtccctgaa cgagcgaatc gagaccctgt
     1801 ctcgtcggat cagcgcgttg gaaggcggga agacctctca ggctgacgac gggcccgccg
     1861 attcgacgcc cgaaccggcc cccgcccgtc gacgcggacg gccgcccaag gccgcgacag
     1921 ccgcggccgc ggccgcggcc aatggagacg ccggaaccgc cgtacccacc gatgcgccga
     1981 ccaagaaggg gaagcggaag aagcaggcgg ctggaaagac gaagcacgcc cgcggcaagg
     2041 gcaagaaccc ggccgtgccg cttcacgagg agatcgcggc cgtcctggcc gagcgtggtc
     2101 cgatgacagc gggcgatctc gcgaaggccg tctcagagcg gggtgtgtac gcaccaccgc
     2161 ggagcgcgaa ggcgctggat gccaccgcgg tcagcgtccg ggtgtcgaac ccgcgctatc
     2221 ggtcgcgctt cacgcgagcg gacgggaaga tcggcctggc ctagtcctcg aggacgaacg
     2281 tgaccaggat gttggcctgg aactcgatcg agccgtcgct gttgatgttg acccgctgct
     2341 ccttgaccca tgcgcccctg accccacgca acgtgcgctg ggcgcgctcg acgccctgac
     2401 gcacggcgtc ttcgaagcta tcgggtgagg tgcagctcag ctcggtgacc ctggcgaccg
     2461 acatcggata gtcctccaac gagacgggtg gagcgccagt ctacgggagg gtcaggatcc
     2521 gtcgtgggtt ctcgaccatg atccgctcga gctcaccctc tccgaccgcc gcggtcctga
     2581 gcgccggcag gaaatgctgt tggagatagg tgtagccgaa gcccccgttc accttcagct
     2641 gcatgtcgtg gcacacatcc tggctgagca ggatctgggc gccgtagccc cgctccagga
     2701 gctcgacgat gagctcgacg atccgcggct cgcgttgctc gtccaccggg tcgaaccgat
     2761 ggccgaggaa gtcgaactgg aggttcgcgc cccgatcgag gatggcgaga tagtgatcga
     2821 ggtccggcca cgagtcggca tggccgatga ccacccggcc ggggtcgaca ccctcggaca
     2881 ggaagatgtc cagctgcgcc tcgcccaccg ccgactggac gccgtgggtg gtgaccgtca
     2941 tcccggtccg gatcgcggcc cgcgccgcgg cccggtgaac gcgctcttcg agcgcgctga
     3001 tccacggctt gtcggtcccg atctcgccga tgatgccggg ctggatgccc gtctcgccga
     3061 cgccgtgctc gaactcgctg accagctcgt ccgccaggct gtccaccgat cggcggtcga
     3121 tgagcgcctc ggccggataa tagggtcccc gataccagcc gcagcccatc acgatctgga
     3181 cgccggtccg gctggcgagg cggcggagcc gctccggatc gcggccgata cctggaacgg
     3241 tgaggtcgac cagggtccgg ccgccgcggc gccgatagtc gcgcagctcg tcggcgatcg
     3301 tgagctcgtc ggccgccagc tcccagtagt cgaaccggcc cgggatgtgc cacagggaga
     3361 cccgtgtgtg ctcgtggagg agcgcaaaac caacggattc gggcggaatc ggcccggaga
     3421 cagtcatcac ccggggagcc atggcggtac ctcggttcgg gcgccggtcg gatggagcga
     3481 accgccggcg tcgcttggat gggttgaccg cggcatggtg ccactcggca cccgagtcgc
     3541 gcaaaagact gttgccgctg gccgtcaaag tgctacaaca cctcggaacg gccgcccgat
     3601 tcgacggcca ggaccatagg aggtcattgg agcgatgaat cagcggccaa ccggggtcac
     3661 catcctggcc gtcctcgcct tcatcggcgg ggcattcggc atcttgggtg cgcttgccct
     3721 cctggggctg ggcgttgcgt ttgccggcgt tgccggaggc gggctcagct tcatattcgg
     3781 gatcgtgttc ctcgccctca gcatcctgta cctgtggatc ggctggggct tctggaacct
     3841 caagcgcacc gcatggagcc ttggcctcgt ggtgttcggg gccggcatcg tgatctcgct
     3901 gatcgagatg ctcctgggct acgccagcat cacgaacctg atcgtaacga tcgtgatcaa
     3961 cggcatcgtg atctactacc tgctcacgcc gggtgtgaag gcggccttcg gagtcgaagg
     4021 cgacctccag agcgcggtca tgagcgggct gccgatggga ggcaccgcct ccacgtcggc
     4081 cccggcggcg actccgccgg cggcacctgc gcctccggcc gctccgcctc cggcagagcc
     4141 accggccagc acgtggagcg gctcgggcga gtcttcgggc ggctccaccc caagcgcggg
     4201 caccgacgac agcgacccca acgcgtagtc gccgaaccag cggtcgcgcg cgccgcgcga
     4261 accgtggcgt gggcgcccga cgggtaaccg tcgaggcgcc cacatcggtc tcttgagcac
     4321 gctgccatga acgaacccga gccctcgtac agcggcacgc aggaatgggc cgccgcccag
     4381 gctgaggtcg acgacgttgc cgcgcgtctg aagctcgacg acgggatgca tcgcgtcctt
     4441 cgcgtcgcca agcgcgagct ggaggtccac ttcccgatcc agttcgatga cggacgggtg
     4501 gacgcggtca ccggctggcg ggtccatcac aacatcaacc gcgggccatc gaccggtggc
     4561 gtgcgataca ccgcggacct caccctggac ctcgtgcggg ccaacgcgat gtggaacacg
     4621 tggaaggcgg ccatcgtcga gattccctat ggcggcgcgt tcggtggcgt agtggtcgat
     4681 cccaagcagc tgtccgccca cgaggtcgag ggcctgaccc gccgctacac gaccgagatc
     4741 agcccgctca tcggcccgtc ccaggacatc cccatgccgg acgtgaacac cgatgagcag
     4801 acgatggcgt ggatgatgga cacgtactcc atgcagcgcg ggtacacgat ccgaggcgtg
     4861 gtgaccggca aaccacctgc gatcggtggc acgcgaggtc agcgtgatgc cgtcagcctg
     4921 ggggccctcc gcgccatcca ggcggtcgcc aggacccagg gcctgtcggt cgacggcgcc
     4981 cgggtcgtga tgcagggctt cggtcgggtg ggcaccatcc tggccgagat gatggccggg
     5041 gccggcgccc ggatcgtggc catcgccgac gaccgtcatg ccatccgcaa cccgtccggg
     5101 atcgaggtcg ggccggcggt ggcgcacatg gccgcccacg acagtgccga gggactgccc
     5161 ggtggtgagc ccatggaccg cgccgacgta tttgcggagc cgtgcgacat cctggtctcg
     5221 gccggtcttc agggtcagat ctcggccacg aacgcaccgc ggatcgaggc ccggatcgtg
     5281 gccgaggccg cgaacggggc gacatcgccg gaggccgatc tcgccctcta cgacaacggg
     5341 gtgacggtga tccctgatct gctgtgcacc gccggcggct tcgtggtcgg ctacctcgaa
     5401 tgggtccagg acctgcagtc gttcttctgg agcgatgccg aggtgaccgc tgagctcgac
     5461 cgggtgatga acgacgcggc tgccgcggtc atggcagccg ccgatcgcca ccacgtggac
     5521 ctccggacgg cggcgctgat ggtcggcatc ggccgggccg cggaggcaac cacgttgcgc
     5581 ggcctatacc cttagggccg ggtggcagag cagcacgacg accgcaccgc gtcacacgac
     5641 cagcccgaac gctacggtgg cgacgggctg gatctcgatc gtgaccggct gacccttcgc
     5701 ttcgaaagct tcgagctcga gcgcacgttc caggtcgacc tcggcctgcg actccgcctc
     5761 cagcaccggg tcggcctgtt cctcgccacc ggcctgtgga tcaccgccgg gctcatgctg
     5821 atcgtgatct acggcgtcga tccgctggag atcgcgctcg ccatctcggt gccgatggcg
     5881 ttcgtgatcg gtgggctggc gatcatcggc cggttcgaga cctgggacgc gcagcaggtc
     5941 gtgaacggga tcatcaacct cgtcggcggg atgtccatga tcttcatctc gacccgggtg
     6001 gcggacgtgc ccgaggccct gggcacggtc ctcatcctga acgcgatctt tgccttcagc
     6061 gtggtacgga tggggttcgt catcggcctg gcagccgagc tgccggtcct catctgggtc
     6121 gccgccctcg cctggatcgg gacctatccc gagatcgggt ggttcacggt cttcctgctc
     6181 gccagcggcc tgggggtcgc cgcctacggg gcatacatgc tcgagtcgtc cgcgcgcgcc
     6241 cgattcctcc agcggcacga cctcgctcgg cagggagagg ccctggcccg cgagaaggcg
     6301 aagtcggacc ggctgctcca caccatgctt cccgcctcga ttgccgaccg cctccgggac
     6361 gagccgcggg tcatcgccga cgcgttcccg gacgccaccg tcgtcttctc cgacatcgtg
     6421 ggcttcaccc cgatcggctc ggacatggca ccggtcgatc tcgtgaccgt cctgaacgag
     6481 ctgttcggtc gcttcgacga gctcgccgcg cgccacggcc tggagaagat caagaccatc
     6541 ggcgacggct acatggcggt cgcgggcgtg ccgctggcga tggacgacca cgccgatcgg
     6601 gccgtccggt tcgggctggc catccatcgc gccacgaacg accttggcgc ggagctgagc
     6661 atgccgcttc agctgcgggt gggcatccac agcgggccgc tggtggccgg ggtcatcggg
     6721 cgcgacaagc tcgcctatga cctgtggggc gacacggtca acgtggccag ccggatggag
     6781 tcgcagggca tgcccgggcg ggtccagatc agcgaggcga ccgcgttgcg cctgaacgag
     6841 gaggtcagcc tcgagccgcg cggcacgatc gaggtgcgcg gtcgggggcc gatgcgcgtc
     6901 ctgctcgtca acgagcccga ccagaccgat ggcgcggtcc cggtcgccga aacagcacgg
     6961 gcatgaatcc ggcaggtcgc ggccgatgag caggaacgag cggaagccgc ggttcacgac
     7021 ccagtccgga gtcgagatca accgcctcta ttcgccggag gacctagccg gctgggatgc
     7081 ggcccgagac
//
LOCUS       DASYGU010000030         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_467655, whole genome shotgun sequence.
ACCESSION   DASYGU010000030 DASYGU010000000
VERSION     DASYGU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467655"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGO32_00745"
     CDS             <1..494
                     /locus_tag="VGO32_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7199312.1"
                     /translation="EAFVAGDLDGAWLPEPWVTRMVDEAGGHVLVDERDLWPDGQFVT
                     THLIVATAFLEAHPDVVKRLLEGHVAATQAVNADPTHAQEVVAAAIADLSGTEVAVGL
                     LASAWDKMTFTIDPIANSLTTSAENAVELGLLDSADLEGIYDLSLLNEVLADAGASPI
                     PGP"
     gene            491..1282
                     /locus_tag="VGO32_00750"
     CDS             491..1282
                     /locus_tag="VGO32_00750"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199313.1"
                     /translation="MTATAARSLDAAPGQGAGARLVIDDVHKRYGPVGNHLLALGGIS
                     LTVNPGEFVCLVGASGCGKSTLLNLIAGLDQPTAGTIESEGRLGLLFQESALFPWLTA
                     AQNVNLALRLADVPAATRDEEAANLLRLVHLQGFGDKRPHELSGGMRQRVALARALAQ
                     RAHLLLMDEPFGSLDAITRDLLHDELERLWMELSFTTLFVTHDVREAVRLGDRVVILS
                     SRPGRVVEEVPVDLPHPRVGVDDDMTEIASMITRRLRQEVQRHGR"
     gene            1272..2135
                     /locus_tag="VGO32_00755"
     CDS             1272..2135
                     /locus_tag="VGO32_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7199314.1"
                     /translation="MAAETQAAERELAGLDSLELPIEPRKSFGERIWSSAWPILVAVT
                     IVLLLWELAVLLELRPTYVLPDPITVLSRLLTDLTTNDLILAVSITLRRAATGFALAL
                     VIGTLIGLAIVRFRLLRAGIASLITGLQTMPSIAWFPLAILVFGLSEAAILFVVILGA
                     APSVANGLISGVDYIPRVLIRAGKVLGAHGLAAYRFVILPAAFPSFISGMKQAWAFAW
                     RSLMAGELLVIIAGQPSIGVRLQLSRELSDAPGLLATMIVVLLVGVVVDLAVFGTMER
                     VVRRRWGLLEA"
     gene            complement(2155..2646)
                     /locus_tag="VGO32_00760"
     CDS             complement(2155..2646)
                     /locus_tag="VGO32_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV7199315.1"
                     /translation="MRVSARADYALRAVAELAAGDGSPVKREQLAERQGIPLDFLEHV
                     LLELKHAGIVMSQRGSTGGFRLGRPAADISLADVIRAVDGPMANVRGRRPEEVDYPGP
                     AEHLQDVWIAVRASLRDLLESVSVEDLVSGELSEQIKALTLRPDAYRSLGRVRGPSRA
                     EVP"
     gene            complement(2681..2756)
                     /locus_tag="VGO32_00765"
     tRNA            complement(2681..2756)
                     /locus_tag="VGO32_00765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2721..2723),aa:Ala,seq:cgc)
     gene            2840..>3200
                     /locus_tag="VGO32_00770"
     CDS             2840..>3200
                     /locus_tag="VGO32_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199316.1"
                     /translation="MTNAAADAPAIRTEGLTKSYGRKRGIIDVDLEVRQGEIFGYLGP
                     NGAGKSTTIRLLLDFIRPSSGTASVLGQPAHGSATKLRRQIGYLPGEMALYPRMTGRD
                     LLRYFAALRGLKDLSHAE"
BASE COUNT          466 a         1147 c         1061 g          526 t
ORIGIN      
        1 tggaggcgtt cgtcgccggc gacctcgacg gagcgtggct ccccgagccc tgggtcaccc
       61 ggatggtcga cgaagccggc ggccatgtcc tcgtcgatga gcgtgacctg tggcccgacg
      121 gccagttcgt gacgacccac ctgatcgtgg caacggcatt cctggaggcg caccccgacg
      181 tcgtgaagcg gctgcttgaa ggccacgtcg cggccaccca ggcggtcaac gcggatccga
      241 cccacgccca ggaggttgtg gccgcagcca tcgccgacct gtcggggacg gaggttgcgg
      301 tcggcctgtt ggccagcgcg tgggacaaga tgacgttcac catcgatccc attgcgaact
      361 ccctgacgac gtcggcggag aacgccgtcg agctcggcct cctcgactcg gccgacctgg
      421 agggcatcta tgacctgtcc ctcctgaacg aggtcctggc cgacgcgggc gcaagcccga
      481 tccctggccc atgacggcaa cggccgcgcg atcgctggac gcggcccccg ggcagggagc
      541 cggggcgcgg ctggtcatcg acgacgtcca caagcgctac gggcccgtcg gcaaccatct
      601 cctggccctg ggcggcatct cgctcaccgt gaatccgggc gagttcgtct gtctcgtcgg
      661 cgcatcgggc tgcggcaaga gcacgctcct gaacctcatc gccggcctgg atcagcccac
      721 tgcgggaacg atcgagagcg aagggcgcct cggtctcctg ttccaggagt ccgccctatt
      781 cccgtggctg accgccgccc agaacgtgaa cctggcgctt cgtctggcgg atgtgccggc
      841 ggccacccgg gacgaggagg cggccaatct cctccgactg gtccacctcc agggattcgg
      901 ggacaagcgc ccacacgagc tgtcgggtgg gatgcggcaa cgggtcgccc tggcccgtgc
      961 cctcgcccag cgggcccacc tgctcctcat ggacgagccg ttcggcagcc tggacgccat
     1021 cacccgggac ctgctccacg atgagctcga gcggctgtgg atggagctca gcttcaccac
     1081 cctgttcgtc acccacgacg ttcgcgaggc cgttcggctg ggtgatcggg tcgtgatcct
     1141 ctccagccgg ccgggccgcg tcgtcgagga agtgccggtt gacctgcccc acccgcgagt
     1201 cggggtcgac gatgacatga ccgagatcgc gtccatgatc acgcggcgcc ttcgacagga
     1261 ggtccagcgc catggccgct gagacccagg ccgccgagcg cgagctcgcc ggcctcgaca
     1321 gcctcgagct gccgatcgag ccccggaagt cgttcggcga gcggatctgg tcgtccgcat
     1381 ggcccatcct ggtggccgtc accatcgtcc tgctcctgtg ggagctcgcg gtcctgctcg
     1441 agctccggcc aacctacgtc ctgccggatc cgatcacggt cctctctcgt ctcctgaccg
     1501 acctgacgac caacgacctg atcctggccg tgagcatcac gctccgccgc gccgcgacgg
     1561 gattcgccct ggcgctggtc atcggcacgc tcatcggtct tgccatcgtc cgattccggc
     1621 tgctgcgtgc cggcatcgcc tccctcatca ccggcctcca gaccatgccg tccatcgcct
     1681 ggttcccgct ggccatcctg gtcttcgggc tgtcggaggc ggccatcctg ttcgtcgtga
     1741 tcctgggcgc cgcaccatcg gtggccaacg gtctcatctc cggcgtggac tacatccccc
     1801 gcgtcctgat ccgcgccggg aaggtcctgg gtgcccatgg cctcgccgcg tatcggttcg
     1861 tgatcctgcc ggcggcattt ccatcgttca tcagcggtat gaagcaggcg tgggccttcg
     1921 catggcggtc cctcatggcc ggtgagctgc tggtcatcat tgccgggcag ccgtcgatcg
     1981 gcgtccggct ccagctgagc cgcgagctgt cggacgcgcc gggcttgctg gccacgatga
     2041 tcgtcgttct gctggttggg gtggtggttg acctggccgt gttcgggacc atggagcggg
     2101 tcgtccgccg tcgctggggt ctcctcgagg cgtaacgggc cggaacgggt cggcctatgg
     2161 cacctcggcg cggcttggac cgcggacccg gcccaggctg cggtacgcat ccggccgcag
     2221 ggtcagcgct ttaatctgct ccgacagctc cccgctgacc aggtcctcga ccgagaccga
     2281 ctcgaggaga tctctcaggc tcgctcggac tgcgatccac acgtcctgga ggtgctcggc
     2341 cggaccgggg tagtcgacct cctccggacg gcggccacgg acgttggcca tcgggccgtc
     2401 cacggcgcga atgacgtccg ccaagctgat gtccgctgcc ggtcgaccga ggcggaaccc
     2461 accggtcgag ccgcgctggc tcatgacgat gccggcgtgc ttcagctcga ggaggacgtg
     2521 ctcgaggaag tcgagcggga tgccctgccg ttcggcgagc tgctcgcgct tgacgggact
     2581 cccatcaccg gcagcaagct ctgccacggc gcggagggcg tagtcggccc gggcggagac
     2641 gcgcactgcc gagcattgtg ccactgcgat cggtgggcaa tggtggagat gaggggactc
     2701 gaacccctga cccccgcgat gcgaacgcgg tgctctccca gctgagctac atccccacgg
     2761 ggcatgtgac gaggcgcccg gcaggatagc acgacccctc tcacgacctg ctcgggcgcc
     2821 ccatagaatg aacggaccga tgacgaacgc cgccgcagac gcgcctgcca tccggaccga
     2881 gggactgacc aagtcctacg gtcgcaagcg cggcatcatc gacgtcgatc tcgaggtccg
     2941 gcagggcgag atcttcggct atctcgggcc caacggcgcc ggcaagtcca cgaccatccg
     3001 cctgctcctg gacttcatcc gcccgtcgag cggcacggca agcgtgctcg gccagcccgc
     3061 ccacggatcc gcgaccaagc tccgacggca gatcggctat ctccccggcg agatggccct
     3121 ctacccacgg atgaccgggc gcgacctgct ccgctacttc gccgccctgc gcggcctgaa
     3181 ggatctgtcg cacgccgagg
//
LOCUS       DASYGU010000031         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1496595, whole genome shotgun sequence.
ACCESSION   DASYGU010000031 DASYGU010000000
VERSION     DASYGU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496595"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGO32_00775"
     CDS             <1..777
                     /locus_tag="VGO32_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199317.1"
                     /translation="ADVFVGHDEVLSRHGIWTPDHAGRRPLRPVLAPTPESLIVAEQL
                     SAQPALAERPLFARLDLSVRSAEVLAIVGPNGSGKSTLARLLGGLLRPQVGTVTASEP
                     MAPGRAGSVIWQWPARSLVSRIGSVFQDPEHQFLTGRVSDELMLGPIRAGLAETEAHR
                     RATELADRLRLDPVWDANPFTLSGGEKRRLSVATALATAPRVVILDEPTFGQDRRTWD
                     EMLTLLAGLADSGRAVVMATHDRALVDALADRTLRLGPED"
     gene            781..1566
                     /locus_tag="VGO32_00780"
     CDS             781..1566
                     /locus_tag="VGO32_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEV7199318.1"
                     /translation="MQLGRPAMANPAAFLPSANPIAKLAAAVFVMVVAFASRDPVTPA
                     ILLAGEGLALLLTGIHPRALVRMVGPLIVVAGMLGLLNALLVGLPVQGPAAGEPDRLA
                     TGLGLGLRIAAIALAGVLALATTDPADLAAGLIGHLHIPPRLAVGALASLRLVPILGT
                     EWQTIGLARRARGVDAGRNLLVAGRLALQAVVTLLVSTIRRASRLATAMDARGFDSGL
                     PRTMARPPRMRRTDWWLVVAAAGLALGAVAVSVGLGTWTFIFG"
     gene            1595..2575
                     /locus_tag="VGO32_00785"
     CDS             1595..2575
                     /locus_tag="VGO32_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7199319.1"
                     /translation="MARNEELSIAAGRDTLRGTLTLPEPREVDDAGDDRWPAALLVAS
                     FLPRDRDGRLDRARHAGWFADPGPDDGILARLADALAARGVASLRYDKRGCGQSDGEW
                     ETAGAFTLVDDARDAIAALRGHDAVDAVRIGIVGHGEGGWLALSVAAADPGIGPLTLI
                     GSPARGLRDVLRRAVAERSRRDRGSHEEPRHPFVAALDRGIEELVERAHRGETEMGLT
                     LTAGQRVTLGLAGWEQAMQIPARALATLQQRSVTVVHGAADRWVDPDEATLLAAALMP
                     TAAPRRITVAAAGHDLTEATPELFRDLAADLAARLQPRRLPTVLLSIGLR"
     gene            2588..3052
                     /gene="bcp"
                     /locus_tag="VGO32_00790"
     CDS             2588..3052
                     /gene="bcp"
                     /locus_tag="VGO32_00790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEV7199320.1"
                     /translation="MSYPQVGKKAPDFAMHADDGSLVTSESLAGRRYVLYFYPKDDTT
                     GCTAQACSLRDNWEVAEALDAEIFGVSPDSVASHVKFREKYGLRHRLLSDTGHAVADA
                     YGTWVEKSYRGRTYLGVERTSFVIGADGRIEHVLPRVKPMEHLGQLVEVLTG"
BASE COUNT          384 a         1209 c         1123 g          462 t
ORIGIN      
        1 gcggacgtct tcgtcgggca cgacgaggtc ctctcccgcc acggcatctg gactccagac
       61 cacgccggcc gccggccgct gcggcccgtt ctggccccca cgccggagtc cctgatcgtg
      121 gccgagcagc tgtcggccca accggccctc gccgagcggc cactcttcgc acgcctcgac
      181 ctgtcggtcc gctcggccga ggtgctggcc atcgtcgggc ccaatgggtc gggcaagtcg
      241 accctcgccc gcctcctcgg cggactgctc cggccgcagg tcgggacggt cacggccagc
      301 gagccgatgg ccccgggccg cgccgggtcg gtgatctggc agtggcctgc ccgcagcctc
      361 gtgagccgca tcggctccgt cttccaggat ccggagcacc agttcctcac cggtcgcgtg
      421 tccgacgagc tcatgctcgg accgatccga gccggactgg ccgagaccga agcccaccgg
      481 cgggcgacgg agctggccga tcgcctccgg ctcgacccgg tctgggatgc gaatccgttc
      541 acgctgtcag gcggcgagaa acgacggctc tcggtggcca ccgcgctggc caccgcgccc
      601 cgggtggtca tcctcgacga gcccacgttc ggccaggacc ggcgtacgtg ggacgagatg
      661 ctgaccctcc tggcaggcct ggcggacagc ggccgagcgg tggtgatggc cacccacgat
      721 cgggcgctgg tcgatgccct cgccgaccgg accctccggc tcggcccgga ggactgaccg
      781 atgcagctgg gccgccccgc catggccaat ccggccgcgt tcctgccatc agccaatccg
      841 atcgccaagc tcgccgcggc cgtgttcgtg atggtcgtgg ccttcgcctc ccgcgacccg
      901 gtcacgcccg cgatcctgct cgccggtgag gggctcgccc tgctgctcac cggcatccat
      961 ccccgcgcgc tcgttcggat ggtgggcccg ctgatcgtgg tggccggcat gctcgggctg
     1021 ctgaacgcgc tgctcgtcgg gttgccggtc caggggcccg cggccggcga gccggaccgg
     1081 ctcgccaccg gcctgggcct cggcctgcgg atcgcggcga tcgcgctcgc cggcgtgctg
     1141 gccctcgcca ccaccgaccc ggcagacctc gccgcgggcc tcatcggcca cctccacata
     1201 ccgccccgcc tcgccgtcgg cgcgctcgcc tcgctgcgcc tggttcccat tctgggcacg
     1261 gagtggcaga cgatcgggct cgcccgtcgc gcgcggggcg ttgatgcggg ccgcaacctg
     1321 ctggtggccg gtcgcctggc gctgcaggcg gtcgtgaccc tgctggtgtc caccatccga
     1381 cgggcctccc ggctggcgac ggccatggac gcccgcggct tcgacagtgg cctgccgcgg
     1441 accatggccc gtccaccccg gatgcgccgc accgactggt ggctggtggt cgccgccgca
     1501 ggactggcgc tgggcgcggt ggcggtgagc gtgggcctcg ggacctggac gttcatcttc
     1561 ggctgaccaa gggggcggtg gcatgatggc caccatggcc cggaacgagg agctctcgat
     1621 cgccgcgggc cgcgacacgc tccgcggcac gctcacgctc cccgagccga gggaggtgga
     1681 tgacgccggc gacgaccgct ggccggctgc gctgctcgtg gcaagcttcc tgccccgcga
     1741 ccgcgacggc cggctcgacc gcgcacgcca cgccggctgg ttcgcggacc cgggacccga
     1801 cgacggcatc ctggcccgcc tggccgacgc gctcgcggcc cgcggggtgg ccagccttcg
     1861 gtacgacaag cgcgggtgcg gccagtcgga cggcgaatgg gagaccgccg gcgcgttcac
     1921 gctggtggac gacgcccgcg atgccatcgc cgcgcttcgg ggtcacgacg cggttgacgc
     1981 cgtccggatc gggatcgttg ggcatggcga gggcggctgg ctggcgctga gcgtcgccgc
     2041 ggccgatccg ggcatcggcc cgctgaccct gatcgggtct ccggcccgcg ggcttcggga
     2101 cgtgctccgc cgagccgtgg cggagcggtc gcgtcgagat cgcgggtccc acgaggaacc
     2161 gcgccatccg ttcgtggctg ccctcgatcg cgggatcgag gagctcgtcg agcgggcgca
     2221 ccgcggcgag accgagatgg gtctcaccct gacggccggc cagcgcgtca cgctcggcct
     2281 ggccggctgg gagcaggcca tgcagatccc ggctcgcgcc ctggccaccc tccagcagcg
     2341 atcggtcacc gtggtccatg gcgcggccga tcgctgggtc gatcccgacg aggcgaccct
     2401 gctcgccgcg gcgttgatgc ccaccgcagc gccccgtcgg atcacggtgg ccgccgccgg
     2461 ccatgacctg accgaggcga ctccagagct cttccgcgac ctcgccgccg acctcgcggc
     2521 ccggctccag cctcgccgcc tgcccaccgt cctgctgtca atcggcctgc gctaggatgg
     2581 cccggccgtg agctaccccc aggtgggcaa gaaggccccg gatttcgcca tgcacgccga
     2641 cgacggcagc ctcgtcacct ccgagagcct cgccgggcgt cgctatgtcc tgtatttcta
     2701 tcccaaggac gacacgaccg gttgcacggc ccaggcctgc tcgctccgcg acaactggga
     2761 ggtggccgag gcactcgacg cggagatctt cggcgtctcg ccggacagcg tggccagcca
     2821 cgtgaagttc cgcgagaagt acggcctccg ccatcggctc ctgtccgaca cgggccacgc
     2881 ggtcgccgac gcctacggca cctgggtcga gaagtcctac cgcgggcgga catacctcgg
     2941 ggtcgagcgg acgagcttcg tcatcggcgc tgacggtcgg atcgagcacg tcctcccccg
     3001 ggtcaagccg atggagcacc tcggacagct ggtcgaggtc ctgaccgggt gaagggccgc
     3061 gaggtggccg ccaccgtggc ggtcgccctg gtcggcctcg ctgtcatcgc cgccgtcctg
     3121 ggcctcgtgg ccctcaccaa tccgattcca tgatctgggt cggcatcggt ctgtgcgt
//
LOCUS       DASYGU010000032         6710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1136822, whole genome shotgun sequence.
ACCESSION   DASYGU010000032 DASYGU010000000
VERSION     DASYGU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136822"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGO32_00795"
     CDS             <1..407
                     /locus_tag="VGO32_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199321.1"
                     /translation="AIAMTSDLLHEAGLAERADLWVGDAAVESLEPHDVVVLDKVICC
                     YPDADALIARSTAAAERIYAFAVPESRGFWGLVARSRWFFYGILEWIHGDRLPRYIHR
                     RTDVEAKVMAAGFRPLHTGRRFVWFVGVYARN"
     gene            complement(404..2512)
                     /gene="ligA"
                     /locus_tag="VGO32_00800"
     CDS             complement(404..2512)
                     /gene="ligA"
                     /locus_tag="VGO32_00800"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV7199322.1"
                     /translation="MTETAGKAGKAAGKADAKRAAELREALEDANYRYHVLDAPNIDD
                     AQYDAFLRELDELEAAHPELLTPDSPTQRVGAAPSAGFAEVRHSVPMLSLSNAFGADE
                     LREFDARVRKGLGLTDDDPGVTYVCELKIDGLAMSLRYEGRTFVRGATRGDGFTGEDV
                     TANLRTVRTIPLTLRADPPGEALEVRGEVFMPRTAFEALNAQLEAEGKPKYANARNTA
                     AGSVRQKDPKATSSRRLSVWMYSLVGVPGLESQSASLALLRELGFAVNPHVRQARGVD
                     EVLAFVEEWQDKRHDLEYETDGVVVKVDEVAQQETLGFVSRSPRWAVAYKFPAEQVTT
                     RLESIEVYVGRKGALTPVAHVAPVFVGGTTVRNATLHNIDEIRRKDLRVGDTVRLQRA
                     GDVIPEVLGPVVEARTGKETVWEMPTTCPACGTEVVRDEGEVIARCPNPFCPAQRLGG
                     LIHFTGRGGMDIEGAGYAVVVQLVDKGLVSEPADLYRLTMEQIEGLDRFALKSAENLF
                     GSIQRARQRPLYRIVNGLGIRHVGEQTAIDLASWLARDVPPAEGESDADWTGRVAERL
                     TNASAEELTSVFGVGKVVAESIALYFRDEHTGEMLRHLVEAGVTAERPAPGVAATEDG
                     PLAGKTVVVTGTLPTFSREDAEAAVRAAGGHPAGSVSAKTDYLVAGEKAGSKLAKAES
                     LGVPVLDEEGFRRLLERGEP"
     gene            complement(2505..3008)
                     /locus_tag="VGO32_00805"
     CDS             complement(2505..3008)
                     /locus_tag="VGO32_00805"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7199323.1"
                     /translation="MTNVDPQVQALATDLVEARDAFRQALADMDPALLTTPGLVGVWS
                     ARELVAHMGYWAGHAAEAIHLAEQGRLDEFEAEVPSVDERNEVVARVAAETDMTTVQS
                     REELAFEAFRQRILTVDPDWLGERDADGDTLEEIIKYDGVDHFREHTNDIRAWFAAGG
                     EPSSADD"
     gene            complement(3036..4151)
                     /locus_tag="VGO32_00810"
     CDS             complement(3036..4151)
                     /locus_tag="VGO32_00810"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEV7199324.1"
                     /translation="MTSRRAPRIGIVTGGGDAPGLNTVIRAVVMAADGRGWETVGILG
                     GFEGLLPPSAHRPLTIRDTDALLFRGGTILGTSSRGRFTDHRRTGGPPTIAPAIVAEA
                     ARATNQMGLEALVCIGGDGSLRIAQALSVAGVPVVGVPKTIDNDLPGTDSTFGFQSAV
                     AVAVDALDRLASTAESHRRVMVLEVMGRHAGWIATHAGVAGGANMILIPEIRFDWDVI
                     GAFIQGRWQAGMSHTLIVVAEGARAIGGRYVTRSGSGGPPGERRLGGIGPRVAAEVQK
                     RTGREARALTLGHLQRGGAPNAWDRQLCARYGVAAVEAVANGQTGTMIALRGGAILPV
                     PLSQGVGRIRTVPVDGELVLSARALGISFGDESASAL"
     gene            complement(4155..>6710)
                     /locus_tag="VGO32_00815"
     CDS             complement(4155..>6710)
                     /locus_tag="VGO32_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV7199325.1"
                     /translation="AAMPPDSTAERALPTTRAIVAPLNPPQREAVEHTDGPLLILAGA
                     GSGKTRVLAHRVAHLVASGVPPYRIVAVTFTNKAAGEMRDRIAGLIGPEATREATIGT
                     FHSICARILRREGDRIGLTSGFTIYDRADQLAVIKNVLRQLDLDDKRFAPPALLSFIG
                     QRKDELADPATAARQADNFWEEQAARVYEAYQRDLAESDAADFDDLLLRVVFLFEQHP
                     DVLARYQRRWQYILVDEYQDTNRTQYRLCALLAAVHRNLAVVGDDDQSIYSWRGADLR
                     NILDFERDWPKAKVVKLEQNYRSTQTILDAAHAVVSRVEGRKDKRLWTDRGSGSAIQV
                     FDAYNEYEEAEFVARQVERLASSADRRRGWSSLLTTRADDEPAAGESGVDGAAGPMKF
                     GDIAVAYRVNAQSRVLEEAFMRFGIPYQLVGGVRFYERREVKDALGYARLARNLGDRV
                     ALERVINVPARGIGDKTVSELRAWADHRGATMWAAVEAAESNANLATRARTAIAGFAE
                     LMRGLAALAAAEPTSAVLEECLERSGLRAVLDDGTEEGTDRWANLVELRNHAAEFDEL
                     EPPEGLARFLEEVALVSDQDTLEERPERVTLITLHAAKGLEFPVVFMVGMEEGLLPYA
                     RALDDERELEEERRLTYVGMTRAKDRLYLVHARHRSAWGSGGVEVEPSRFLADLPEDL
                     LEVGSGRRGPISQGWVREESGAPTRARRERGGTGATWTGAPEWRGGGLDDEASESTTV
                     WGRARPGLEPLRPERLPDLVIPERSALPDVDLGSGTFGRVGDHADGSTEAVDATYATL
                     TWHAGDKVRHRRFGDGIVVSSQWVSGDEEVTVAFAGQGVRRLVGSYAGLERMA"
BASE COUNT          935 a         2532 c         2209 g         1034 t
ORIGIN      
        1 aagcaatcgc gatgacctcc gacctcctcc acgaagctgg gctggccgag cgtgcggacc
       61 tgtgggtcgg cgacgcggcg gtggagtcgc tcgagcccca tgacgtggtc gtcctggaca
      121 aggtgatctg ctgctatccc gatgcggatg cgctcattgc ccgctccacg gccgcggcgg
      181 aacggatcta tgcgtttgcc gtcccggagt cgcgtggctt ctggggtctc gtggcccgtt
      241 cccggtggtt cttctacgga atcctcgagt ggatccacgg cgaccggctg ccgcgctaca
      301 tccaccgccg gaccgacgtc gaggccaagg tcatggccgc cggcttccgg ccgctccata
      361 ccgggcggcg attcgtctgg ttcgtcgggg tctacgcccg gaactagggc tcgccgcgct
      421 ccagcaggcg gcggaatccc tcctcgtcca gcaccggcac gcccagcgac tcggccttgg
      481 ccagcttgga gcccgccttc tcacccgcga ccaggtaatc ggtcttggcg ctgaccgacc
      541 cggcaggatg accgcccgcg gcccggacgg cggcttcggc gtcctcgcgg ctgaacgtgg
      601 gcagggtgcc ggtcacgacc acggtcttgc cggccagcgg accatcctcc gtggccgcca
      661 ccccgggcgc gggccgctcg gcggtgacgc cggcctcgac caggtggcga agcatctccc
      721 cggtgtgctc gtcacggaag tagagggcga tgctctccgc cacgaccttc ccaaccccga
      781 acaccgacgt cagctcctcg gccgaggcat tcgtcagccg ttccgcgacc cgcccggtcc
      841 agtccgcgtc cgattcgccc tcggccggcg gcacgtccct cgccagccag ctggccaggt
      901 cgatggccgt ctgctccccg acatgacgga taccgagccc gttcacgatc cgatacagcg
      961 gccgctgccg cgcccgctgg atggagccga acaggttctc cgcgctcttg agcgcaaatc
     1021 ggtccagccc ctcgatctgc tccatcgtga gccggtacag gtccgcgggc tcgcttacca
     1081 gccccttatc cacgagttgg acgacgaccg cataccccgc gccctcgatg tccatcccgc
     1141 cgcggcccgt gaagtggatc aggccgccca gccgctgggc ggggcagaac gggttcgggc
     1201 agcgggcgat gacctcacct tcgtcgcgca ctacctccgt cccgcacgcc gggcacgtgg
     1261 tgggcatctc ccacaccgtc tccttgccgg tccgggcctc gaccaccggc cccagcacct
     1321 ccgggatgac gtcgcccgcg cgctggagac gaaccgtgtc gcccacccgc aggtccttgc
     1381 gccggatctc gtcgatgttg tgcagcgtcg cattccgcac cgtcgtgccg cccacgaaca
     1441 ccggcgcgac gtgcgcgacc ggagtcagcg cccccttccg gccgacgtac acctcgatcg
     1501 actcgagccg ggtggtgacc tgctcggcgg ggaacttgta ggcaaccgcc cagcgcgggg
     1561 agcgcgagac gaagcccagc gtctcttgct gcgccacctc gtccaccttc acgaccacgc
     1621 catcggtctc gtactccaag tcgtggcgct tgtcctgcca ctcctccacg aaggccagca
     1681 cctcgtccac gccgcgcgcc tggcgaacgt ggggattcac cgcgaacccg agctcgcgca
     1741 ggagcgcgag cgacgccgac tggctctcga gtccgggcac gcccaccagc gagtacatcc
     1801 agaccgatag gcgacggctg gacgtcgcct tgggatcctt ctgccggacc gatcccgcgg
     1861 cggtattccg cgcgttcgcg tacttcggct tgccctcggc ctcgagctgg gcgttcagcg
     1921 cctcgaatgc cgttcgtggc atgaacacct cgccccgcac ttcgagcgcc tcccccggcg
     1981 ggtccgcgcg cagggtgagc gggatggtcc gaaccgttcg caggttggcg gtcacgtcct
     2041 cgccggtgaa gccgtctccg cgggtcgcgc cccgcacaaa ggttcggccc tcgtatcgca
     2101 ggctcatggc cagcccgtcg atcttcagct cacacacgta ggtcacgccc ggatcgtcat
     2161 cggtcaggcc caggcccttg cgcacccgcg cgtcgaactc gcggagctca tcggcgccga
     2221 aggcgttgga cagcgacagc atcggtaccg agtggcggac ctcggcgaag ccggccgacg
     2281 gggccgcgcc cacccgctgg gtcggggaat cgggagtcag gagctcgggg tgggcggcct
     2341 ccagctcgtc tagctcgcgg aggaaggcgt cgtactgggc gtcgtcgatg ttcggcgcgt
     2401 cgagcacgtg ataccgatag ttcgcgtcct ccagcgcctc gcgcagctcc gcggcgcgct
     2461 tcgcatcggc cttcccggcg gcctttccgg ccttcccggc ggtctcagtc atccgccgat
     2521 gagggctcac ccccggcggc gaaccaggcc cgtatgtcgt tcgtgtgctc gcggaagtgg
     2581 tcgacgccgt cgtacttgat gatctcctcc agcgtgtcgc cgtccgcgtc ccgctcgccc
     2641 agccaatcgg ggtcgacggt caggatccgc tgccggaagg cctcgaaggc gagctcttcg
     2701 cggctctgga cggtcgtcat gtcggtctcg gccgcgaccc gggcaacgac ctcgttccgc
     2761 tcgtccacgc tcgggacctc ggcctcgaac tcgtccagcc ggccctgctc ggccaggtga
     2821 atggcctccg ccgcgtgacc ggcccagtag cccatgtgcg cgaccagctc gcgcgccgac
     2881 cacacgccca ccagcccggg cgtggtcagg agggccgggt ccatgtccgc gagtgcctgc
     2941 cggaaggcat cccgcgcttc caccaggtcg gtcgccaggg cctgtacctg ggggtcgaca
     3001 tttgtcatcg ccgccgatgg tagcgcgggg ctgccctaga gggccgacgc cgactcgtcg
     3061 ccgaagctga tgccgagggc gcgggccgac agcaccagct ccccgtcgac cgggaccgtg
     3121 cgaatccggc cgacgccctg ggacagcggc acgggcagga ttgcccctcc ccggagtgcg
     3181 atcatcgtcc cggtctgccc attggccacc gcctcgaccg cggccacgcc gtagcgagcg
     3241 cagagttggc ggtcccacgc gttcggagcc ccacctcgct ggagatggcc gagggtcagg
     3301 gcccgcgctt cccgccccgt ccgcttctgg acctcggccg ccacccgggg accgatcccg
     3361 cccagccgac gttcgccggg cggcccgcca ctcccggatc gcgtcacgta tcgaccgccg
     3421 atggcccgcg cgccctccgc caccacgatc agggtgtggc tcatccctgc ctgccatcgc
     3481 ccctggatga acgccccgat cacgtcccag tcgaaccgaa tctcgggaat gaggatcatg
     3541 ttcgcaccgc ccgcgacccc ggcatgggtt gcgatccagc ccgcatgccg acccatgacc
     3601 tcgagcacca tcacccggcg atggctctcc gcggtgctgg ccaggcggtc cagcgcatcg
     3661 accgccaccg cgacggccga ctggaagccg aacgtgctgt cggtccccgg cagatcgttg
     3721 tcgatcgtct ttggcacgcc caccaccggc acgccggcca cagacagcgc ctgtgcgatc
     3781 cgcagcgaac catcaccgcc gatgcagacg agagcctcga ggcccatctg gttcgtcgcg
     3841 cgtgccgcct ccgcgacgat ggccggcgcg atcgtgggcg gacccccggt gcgccgatgg
     3901 tcggtgaacc ggcctcggct ggacgtgccg aggatggtgc ccccacggaa gaggagggca
     3961 tcggtgtcgc ggatggtgag cggccggtgc gccgatggcg gcaggagccc ttcgaacccg
     4021 ccgaggatgc caaccgtctc ccagccgcgt ccgtccgcgg ccatgaccac ggcccggatg
     4081 accgtgttca ggccgggcgc gtcaccgccg ccggtgacga tcccgatccg tggcgctcgg
     4141 cggctcgtca cggcctaggc catccgctcg aggccggcat aggagccgac cagacgacgg
     4201 accccctgcc cggcgaaggc aaccgtgacc tcctcgtcgc cgctcaccca ctgcgagctg
     4261 accacgattc catcgccaaa gcgacgatgg cggaccttgt cgccggcgtg ccaggtcagc
     4321 gtcgcgtagg tggcatcgac cgcctccgtc gacccgtcag catggtcgcc gacccggccg
     4381 aacgtgccgc tgccgagatc cacgtccggc agggcagagc gctccgggat gacgaggtcg
     4441 ggcaggcgct ccgggcgaag aggctcgagc ccgggacggg cccggcccca aaccgtggtg
     4501 gattcgctcg cctcgtcatc aagcccacca ccgcgccatt ccggcgcccc ggtccacgtg
     4561 gccccggttc caccgcgctc ccgccgagcg cgggttggcg ccccggactc ctcgcgcacc
     4621 cagccctggc tgatcggccc ccgccgtccg ctgcccactt cgagcaggtc ctcgggcaga
     4681 tcggccagga agcgagaagg ctcgacctcg actccgccac tcccccacgc ggaccgatgt
     4741 cgagcgtgga cgaggtacag gcggtccttg gcgcgggtca tgcccacgta cgtgagccgt
     4801 cgctcctcct cgagctcccg ctcgtcgtcg agggcgcggg catagggcag caggccctcc
     4861 tccatcccga ccatgaacac gaccgggaac tccagaccct tcgccgcgtg gagcgtgatc
     4921 agcgtcaccc gctcgggccg ctcctccagc gtgtcctggt ccgacacgag cgcgacttcc
     4981 tccaggaagc gggccagtcc ctcgggcggc tccagctcgt cgaactcggc tgcgtggttc
     5041 cggagctcga ccaggttcgc ccaccgatcg gtcccctcct cggtcccgtc gtccagcacc
     5101 gcccgcaggc cgcttcgctc gaggcattcc tcgagcacgg ccgaggtggg ctcggcggcc
     5161 gccagcgcgg ccaggccgcg catgagctcg gcaaagccgg cgatcgcggt tcgggcccgc
     5221 gtcgcgaggt tggcgttgct ctccgcggcc tcgaccgcgg cccacatcgt ggccccgcgg
     5281 tgatcggccc aggcgcggag ctcgctcacg gtcttgtcgc cgattccccg ggccgggacg
     5341 ttgatcaccc gctccagtgc caccctgtca cccaggttcc gcgccagccg ggcgtagccc
     5401 agtgcgtcct tcacctcgcg ccgctcgtag aaccggacgc cgcccaccag ctggtaaggg
     5461 atcccgaacc tcatgaatgc ctcttccagg acccgggact gggcgtttac ccggtatgcg
     5521 accgcgatgt ccccgaactt catcggtcct gccgcaccgt caacccccga ctcccccgcc
     5581 gccggttcgt catcggcccg ggtggtgagc agcgacgacc atccccgccg acggtccgcg
     5641 gacgaggcca ggcgctccac ctggcgggcc acaaactcgg cctcctcgta ctcgttgtac
     5701 gcatcgaaga cctggatcgc gctgcccgaa ccccgatcgg tccatagccg cttgtccttc
     5761 cggccctcca cccgcgacac gaccgcgtgc gccgcatcca ggatcgtctg ggtcgatcgg
     5821 tagttctgct cgagcttgac gaccttggcc tttggccagt cccgctcgaa gtcgaggatg
     5881 ttgcgcaggt ccgcgccgcg ccaggagtag atgctctggt cgtcgtcacc gaccacggcc
     5941 agattgcggt ggaccgcggc cagcagggcg cagagccggt actgggtccg gttcgtgtcc
     6001 tggtactcgt cgacgaggat gtactgccag cgccgctggt agcgggccag gacgtcggga
     6061 tgctgctcga acaggaatac gacccggagc aggaggtcgt cgaagtccgc ggcgtcgctc
     6121 tcggccaggt cccgctggta ggcctcgtac acgcgggccg cctgctcctc ccagaagttg
     6181 tcggcctggc gggcggcggt ggccggatcg gccagctcgt ccttgcgctg accgatgaac
     6241 gacaggagcg ccggcggcgc gaaccgcttg tcgtcgaggt ccagctgacg gaggacgttc
     6301 ttgatgaccg cgagctgatc ggcgcgatcg tagatcgtga agccggaggt caggccgatc
     6361 cgatcgccct cccgacggag gatccgggcg cagatcgaat ggaacgtgcc tatcgtcgcc
     6421 tcgcgggtgg cctcaggacc gatgagcccc gcaatccggt cgcgcatctc ccccgccgcc
     6481 ttgttcgtga atgtgaccgc cacgatccga tacgggggca cgccggaggc caccaggtgc
     6541 gcgactcggt gggcgaggac ccgggtcttc ccgctccccg cgccggccag aatcaggagg
     6601 gggccgtcgg tgtgctccac cgcttcccgc tggggcgggt tgagcggcgc gacgatcgcc
     6661 cgagtggtgg gcaatgcgcg ctcggcggtg gagtcggggg gcatcgcggc
//
LOCUS       DASYGU010000033         7296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_571332, whole genome shotgun sequence.
ACCESSION   DASYGU010000033 DASYGU010000000
VERSION     DASYGU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571332"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGO32_00820"
     CDS             complement(<1..414)
                     /locus_tag="VGO32_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7199326.1"
                     /translation="MRSVTYSMSVSLDGYIVGPDGGFDWTVPSAEVFRFWIDEIRGIG
                     VHLLGRRLYETMLYWETADQDRSLDDAELEWAALWNPLPKVVFSTTLSAVEGNARLVS
                     GGLAEEIEQLRAEPGEGDIAIGGATLAAEAAALDLI"
     gene            complement(507..583)
                     /locus_tag="VGO32_00825"
     tRNA            complement(507..583)
                     /locus_tag="VGO32_00825"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(547..549),aa:Glu,seq:ctc)
     gene            complement(670..1764)
                     /locus_tag="VGO32_00830"
     CDS             complement(670..1764)
                     /locus_tag="VGO32_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEV7199327.1"
                     /translation="MKVSWPQVLAWRLSRQYLDPIGTASVEDVVRRLGAVQAQVPASA
                     ELAVRLRRADSTAGEVDAALAEGRIIRAWGMRGAVHLLTPEDGGAYLALKAAKRQWEL
                     PSWQSFYGLAPGDWEPFRQAVREALADGPMTPSELGAAVTARPAFRHLDFAFARHWHT
                     ILKPLFSQGVVCFGAPRGAQPTFQRLEDNPRWAGLPEPDEAGRRVVETYLRAYGPATI
                     QHIRYWQTAGERKVRDWLVALGDRRARVDLDGETAYVLVDDLDELGATRPTKVVRLLP
                     GYDPWVMGPGTDDSHVVPPARRALISRQAPFVIAGGVVSGTWARTDDEVSISWFSEAG
                     RPPRQGLGEEVDRLATVFGRPLRLTLRPAS"
     gene            complement(1761..3035)
                     /locus_tag="VGO32_00835"
     CDS             complement(1761..3035)
                     /locus_tag="VGO32_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199328.1"
                     /translation="MNPLDLTAVRRRYLVLIGLRWFQTGLLIPVIVLLMVSRGLSLVE
                     IGLVGSVQGLVILILELPTGGLSDSLGRRPTLLLAYVFALAGLATLYIADSVGGFVLA
                     TTLTAIFRALDSGPLEAWYVDATHAMDPEARIESGLAAGSSVLSLGIAAGALLSGALV
                     ALDPLPAVETLALPVLVALALAVVNLGAVLLLLTETRPTSGFRAIASSVRAVPTVVRE
                     GLMLLRRSRVLAAVVAVELFWGFAIIAFETLFPLRLGEVVGSDTEAAALMGPASSAAW
                     FAAAGGAALIAVVSRRIGVARAAIAMRILQGLTIIAMGLVAGVVGLITAYLACYLTHG
                     ASNPLHSTLLHREVDASHRTTVLSLNSMVFHPAAAIGAIVLTAIASETSLSTAMLVAG
                     VACAAAAPLYIPAFRAERRRTEAAVPAVTAGP"
     gene            3188..5509
                     /gene="aceA"
                     /locus_tag="VGO32_00840"
     CDS             3188..5509
                     /gene="aceA"
                     /locus_tag="VGO32_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HEV7199329.1"
                     /translation="MAVIESVAAPVDASSVGEQDEAAKALQAEIAATRLWMDGDRFSD
                     TTRLHTARQVVEQRGTITTDYPIARAAADKLHARLRELFEERRAVTTFGPYSPGQAVA
                     MKRAGIEAIYLGGWATSAKGSADEDPGPDLASYPLGQVPDEAAGLVRALLTADRNQRF
                     ARSRMTDAERQAVPEVDFSPMIIADADTGHGGDAHVRNLIRRFVEAGVPGYHIEDQKP
                     GAKKCGHQGGKVLVPSDEQIKRLNAARFQLDVMGVAGIVVARTDAEAANLLDGTADER
                     DQPFLLGVTNREVPSYKVGYLAILRRLHRAGATDINGHLLYAVPDEEYAQAEAWLEQV
                     GLLAEIDDVGLAHAADPAANPEHTIDTVADRFLTEWQSRANLKTYGQAVADAMAFRAD
                     EGIELAMSVDNWLAFAATASGRAARSRARDMGLDVTWDPELAQTPEGYYQVEGGIPYA
                     IAKSLAAAPFADVLWMETKTADLADARAFAEAIHAVYPDKMLAYNLSPSFNWDTTGMT
                     DEEMRQFPEELARLGYVFNFITYGGHQIDGLAAEEFTTALLEDGMLALARLQRRFRLL
                     ESPYRTPQTLVGGPRADAALLAASGQTATTKAMGVGSTQAQHLVQTEVPPKVLAGWLA
                     SWAEHHHPGGELAVSLRPHTAGSDVLELSITDQAGEKLANVVFTTIADRRGRTILSVR
                     DQNIFDPTLRRKRLMTLLHLFLIYRYRADSIHYLVPTDDNRRQCERMLEMGILAAVAD
                     EVGDIIVAEVGTATVQALVQPDSEERARLVSGA"
     gene            complement(5541..5963)
                     /locus_tag="VGO32_00845"
     CDS             complement(5541..5963)
                     /locus_tag="VGO32_00845"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV7199330.1"
                     /translation="MATQKGMYGQTPTAPTDPDAWLAALDDVRRPQLEELDARIRKVA
                     PGLSRYVDRGFLSYGRYHYQGKSRSGDWMCIALASNKQYISLYAGPIGLEPYGARLPK
                     ANLGRGCIRFKRVSDVDLEVIDEVIRASAATDGQSITY"
     gene            complement(5966..6832)
                     /locus_tag="VGO32_00850"
     CDS             complement(5966..6832)
                     /locus_tag="VGO32_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7199331.1"
                     /translation="MVQAIKRDEVFIEPEGIHVETHQPERRSRKPPLLLVHGVLTGSW
                     LWASFAAYFAERGWEAHALNLRGHFTSDLTELAETRMRDYANDIGVAARQLGRPPILI
                     GWGMGALAALLYAETRPVLGLVLLAPSPPAAALPRRPADHELKSVPPVYDAEWWGWRA
                     PQEELQAWMPDMSTEDLSKMQELLVGARESGLARQERMLGVDIDPNRIQVPTLVIGAG
                     LDDVIHPSEARRTADLLGATYEYIPGASHFGLVMGSETWPLVAGSILGWLEERRHGMM
                     AAQADLVGAGRR"
     gene            complement(6839..>7296)
                     /locus_tag="VGO32_00855"
     CDS             complement(6839..>7296)
                     /locus_tag="VGO32_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199332.1"
                     /translation="QAVPVAPEALGERMADIRDAEEAVFGLVLPDGEGWLVIGDVDAA
                     ADRMRREPGSEPARTLDLALLHAAVLGDRLGISAADVAAGEMLLYTRSEADARGRVAR
                     GEVAAAILVRPTRLEELTAVAAAGDVMPQKSTFFYPKLLTGMVFYPLEG"
BASE COUNT         1223 a         2527 c         2519 g         1027 t
ORIGIN      
        1 gatcaggtcc agcgcggccg cttcagcagc gagggtcgcg cctccgatcg cgatatcgcc
       61 ctcgcccggc tcggcccgca actgctcgat ctcctccgcc aggccgccgg agaccaggcg
      121 ggcattgccc tccaccgccg acagcgtcgt ggaaaacacc accttgggga gcggattcca
      181 gagcgcggcc cactcgagct ctgcgtcgtc gagcgatcga tcctggtcgg cggtctccca
      241 gtacagcatc gtctcgtaca gccgtcgtcc cagcagatgg acgccaatcc ctcgaatctc
      301 gtcgatccag aagcgaaaga cctccgcact gggcaccgtc cagtcgaagc cgccgtccgg
      361 cccgacgatg tagccgtcaa gtgagacgct catcgaataa gtcacgctgc gcatcagcag
      421 tcctcctcgc gtacggcttc tacgacctga gcgctgtcgc cgcgtgcaac gcctgacccg
      481 atcggatcag gcctcgtgag ccaagttggt accgcatacc ggattcgaac cggtgatctc
      541 cgccttgaga gggcggtgtc ctaggcctct agacgaatgc ggcacggcgt ctctggcgtc
      601 cgggctggac cgggcgggag tatagcaagg gcgctcgaga gctccccaga gacctcttgg
      661 cgaatgcatt caggatgcgg gtcgcagcgt caggcggagc ggccgaccga agaccgtggc
      721 caggcgatcc acctcctcgc cgaggccctg cctaggaggc cgcccggcct cggaaaacca
      781 gctgatcgaa acctcgtcat cggtgcgtgc ccaggtcccg gacaccacgc ctccagcgat
      841 gacgaacggc gcctgacggc tgatcagcgc tcgccgggct gggggcacga cgtggctgtc
      901 gtcggtaccc ggacccatca cccacgggtc gtacccgggg aggagacgca caaccttcgt
      961 cggtcgggtc gcgcccagct cgtcgaggtc gtcgacgagg acgtaggcag tctcgccgtc
     1021 gaggtcaacg cgcgcccggc ggtcgcccag ggcgacgagc cagtcccgca ccttcctctc
     1081 accggcggtc tgccaatacc gaatgtgctg gatggttgcc ggtccgtagg cgcggagata
     1141 ggtctcgacg acccgcctcc cggcttcgtc gggctcgggg aggccggccc atcgcgggtt
     1201 gtcctcgagc cgctgaaacg tcggttgggc cccgcgcggg gcgccgaagc acacgacacc
     1261 ctgcgagaac aacggcttga ggatggtgtg ccagtgcctc gcgaaggcaa agtcgaggtg
     1321 ccgaaaggcc gggcgggcgg taaccgcggc tcccagctcc gatggcgtca tcggtccgtc
     1381 cgccagcgcc tctcgcaccg cctgccggaa aggttcccag tcgcccgggg ccagcccata
     1441 gaagctctgc cagctgggca gctcccactg ccgcttggcg gccttcagcg ccaggtaggc
     1501 gcctccgtcc tccggggtca ggagatgaac ggcgccacgc atcccccagg ccctgatgat
     1561 tcgaccctct gccagcgcgg catcgacttc gcccgcggtc gagtccgccc ggcgcaatcg
     1621 cacggccagc tcagccgatg cgggcacctg ggcctggacc gcacccagcc ggcggaccac
     1681 gtcctcgacg gacgcggtcc cgatgggatc caggtactgc cggctgagtc gccaggcgag
     1741 gacctgtggc cagctgacct tcacggcccg gcagtcacgg caggcacggc ggcctcggtc
     1801 cgtcgccgtt cggcccggaa cgccgggatg tacaagggcg ccgcggcggc gcacgccacg
     1861 ccggcaacga gcatggcggt cgagagcgag gtttcgctgg cgatcgcggt cagcacgatc
     1921 gccccgatcg cggcggcggg gtggaacacc atcgagttca gcgaaagaac cgtggtccgg
     1981 tggctggcat ccacctcgcg gtggagcagg gtcgaatgca acgggttcga ggcgccgtgg
     2041 gtcaggtagc aggccaggta cgcggtgatc agtcccacca ccccggcgac caggcccatg
     2101 gcgatgatgg tcaggccctg caggatgcgc atcgcgatcg cggcccgggc gacgccgatc
     2161 cgccggctca cgaccgcaat caacgccgca ccgcccgccg ccgcgaacca cgcggccgag
     2221 ctcgcgggtc ccatcagggc ggcggcctcg gtgtcgctgc caacgacttc gcccaggcgg
     2281 agcgggaaca aggtctcgaa cgcgatgatc gcaaaccccc agaacagctc gacggcgacc
     2341 acggccgcca gcacccgcga ccggcggagc agcatgaggc cttcgcggac gacggtcggg
     2401 actgcgcgca cggaggacgc gatggcgcgg aatcccgagg tggggcgggt ctccgtcagg
     2461 aggagcagca ccgcccccag gttgacgacc gcgagcgcga gcgccacgag gaccggcagg
     2521 gcgagggtct ccacggccgg caacggatcg agtgcgacca gggcacccga caggagcgcg
     2581 ccggccgcga tgcccaggct gaggaccgag ctgccggcag ccaggcccga ctcgatccgc
     2641 gcctccgggt ccatggcgtg ggtcgcgtcc acgtaccacg cctccaacgg cccgctgtcg
     2701 agcgcccgga agatcgcggt cagggtcgtg gccaggacga atcctcccac cgagtcggcg
     2761 atgtacagcg tcgcgaggcc ggccagggcg aaaacgtacg ccagcagcag cgtgggccgg
     2821 cggcccaggg aatcggacag cccgccggtc ggcagctcca ggatcaggat gacgagaccc
     2881 tggaccgacc cgacgaggcc gatctcgacc agcgacaggc cgcgcgagac catgagcagg
     2941 acgatgaccg ggatcagcag gccggtctgg aaccagcgca agccgataag gaccagatag
     3001 cggcggcgga cggccgtcag gtcaagcggg ttcatggaga gggaaccgcg cgaaccatcg
     3061 gttggttgcc aggcaggagc gccgagaggc aggtgccagc gcgcaccgga gacacggcga
     3121 tcgtcttgac cagcaccgga ccgtgggcca cgctatggcg catcgtgatc gcgattctga
     3181 cgcccgcatg gcagtaatcg aatcggtcgc cgcgccagtt gacgcatcgt cagttggtga
     3241 acaggacgag gcggcgaaag cgctccaggc ggagatcgcg gcgacgcgcc tctggatgga
     3301 cggcgatcga ttctcggaca cgacgcggct gcataccgca cgccaggtcg tcgagcagcg
     3361 cggcacgatc acgaccgact acccgatcgc ccgggcggct gccgacaagc tccacgcgag
     3421 gctgcgcgag ctgttcgagg aacgccgcgc ggtcacgacc ttcggaccgt attcgccggg
     3481 ccaagcggtg gcgatgaagc gggccggcat cgaagcgatc tacctcggcg gctgggccac
     3541 ctcggccaag ggatcggccg atgaggatcc cggccctgac ctggcgagct acccattggg
     3601 ccaggttccg gatgaggccg ccggactggt ccgcgcgctc ctgaccgccg accggaatca
     3661 gcggttcgcc cgatcgcgga tgaccgatgc cgaacggcag gccgtcccgg aggtcgactt
     3721 cagcccgatg atcatcgccg acgccgacac cggccacggt ggtgacgctc acgtccgcaa
     3781 cctgattcga cgcttcgtgg aggccggcgt gcccgggtac cacatcgagg accagaaacc
     3841 gggcgccaag aagtgcgggc accagggcgg gaaggtgctc gttccgagcg acgagcagat
     3901 caagcgcctg aacgccgccc gcttccagct cgatgtcatg ggggtcgcgg gcatcgtcgt
     3961 cgcgcgcacc gacgccgagg ccgcgaacct gctcgatggc acggcggacg agcgcgatca
     4021 gccgttcctg ctcggtgtca ccaatcgcga ggtgccgtcg tacaaggttg gttacctggc
     4081 aatcctgcgg cgattgcacc gggctggcgc cacggacatc aacggccatc tgctgtacgc
     4141 ggtccctgac gaagagtacg cacaggccga agcctggctg gagcaagtgg gcctgttggc
     4201 cgagatcgat gacgtcggtc tggcgcacgc cgccgacccg gccgccaacc cggagcacac
     4261 gatcgacacg gtggccgacc ggttcctcac cgagtggcag tcgcgggcca atctcaagac
     4321 gtacggccag gctgtcgcgg acgcgatggc cttccgggcc gacgagggca tcgagctggc
     4381 gatgagcgtc gacaactggc tggccttcgc cgcgaccgcc tcgggtcgcg cggcccgatc
     4441 acgcgcgcgc gatatgggcc tcgacgtgac gtgggatccc gagctggccc agacgccgga
     4501 ggggtactac caggtggagg ggggtatccc gtacgcgatc gcaaagtcct tggcggctgc
     4561 accgttcgcg gacgtgctct ggatggagac caagacggct gacctcgcgg atgcccgggc
     4621 gttcgcggag gcgattcatg ccgtgtatcc ggacaagatg ctggcctaca acctgtcgcc
     4681 gtcattcaac tgggacacga ccggcatgac cgatgaggag atgcggcagt tccccgagga
     4741 gctggcgcgg ctgggctacg tgttcaactt cattacgtac ggcggccatc aaatcgacgg
     4801 cctcgcggcc gaggagttca cgaccgcgtt gctcgaggac ggcatgctgg ccctcgctcg
     4861 cctccagcgg aggttccgcc ttctggagtc gccctatcgg accccgcaga cgctcgtcgg
     4921 gggcccacgc gccgatgctg cgctgctggc tgcgtctggc cagacagcca cgaccaaggc
     4981 gatgggcgtg ggctcgaccc aggcccagca cctcgtccag accgaggtgc ctccgaaggt
     5041 gctcgccgga tggctggcga gctgggcgga acatcaccac cccggcggcg aacttgccgt
     5101 atcgctgcgg ccgcacaccg ccggctccga tgtgctggag ctgagcatca ccgatcaggc
     5161 gggcgagaaa ctggcgaatg tcgtgttcac caccatcgcc gatcgacgcg gccggacgat
     5221 cttgtcggtg cgcgatcaga acatcttcga tccgaccctg cgacgtaagc gcctcatgac
     5281 cctgctccat ctcttcctga tctatcgcta tcgcgccgat tccatccact acctcgtgcc
     5341 caccgacgac aaccggcgcc aatgcgagcg gatgctggag atgggcatcc tcgcggcggt
     5401 ggccgacgag gtgggcgaca tcatcgtggc cgaggttggc acggcgacgg ttcaggctct
     5461 cgtccagccc gacagcgaag agcgagcgcg gctcgtcagc ggcgcctgac ccaggcgagg
     5521 ccgatcgcgg cctgcaggcg ctagtaggtg atcgactggc cgtcggtggc ggccgaagcg
     5581 cggatgacct cgtcgatcac ctcgagatcg acgtcggaca cgcgcttgaa tcggatgcaa
     5641 cctcggccca ggttcgcctt gggcagcctc gcgccgtacg gctcgaggcc gatgggaccg
     5701 gcgtacagcg agatgtactg cttgttcgag gcgagggcga tgcacatcca gtcgcccgat
     5761 cgggacttgc cctggtagtg gtagcggccg tagctcagga atccgcggtc gacgtatcgg
     5821 ctcaggccgg gagccacctt ccggatcctc gcatccagct cctcgagctg cggacggcgg
     5881 acgtcatcca gcgccgccaa ccaggcgtcg ggatcggtcg gggcggtcgg cgtctggccg
     5941 tacatgccct tctgcgtagc catggtcagc ggcggccggc gcccaccagg tcggcctggg
     6001 cggccatcat cccgtgccgt cgctcctcga gccagccgag gatcgacccc gcaacgaggg
     6061 gccatgtctc cgagcccatg accagcccga agtggctggc gccggggatg tactcgtagg
     6121 tcgcgccgag cagatcggcc gtgcgacgcg cctcggacgg gtggatgacg tcgtccagcc
     6181 ccgcgccgat gacgagggtc gggacctgga tccggttggg atcaatgtcc acgcccagca
     6241 tccgctcctg gcgggcgagt ccagactcgc gcgccccgac gagcagctct tgcatcttcg
     6301 acaggtcctc ggtcgacatg tccggcatcc aggcctggag ctcctcctgc ggggcgcgcc
     6361 agccccacca ttcggcgtcg tacaccggcg ggacggactt cagctcgtgg tccgcgggtc
     6421 ggcggggcag ggccgcggcc ggcggcgagg gggccagcag gacaagcccg agcaccgggc
     6481 gggtctccgc gtacaggagg gcggcgaggg cgcccattcc ccagccgatg aggatcggcg
     6541 gccgacccag ctgccgcgcg gcgaccccga tgtcattcgc gtagtcgcgc atccgggtct
     6601 cggccagctc ggtcaggtcc gaggtgaagt ggccgcgcag gttgagggcg tgggcctccc
     6661 aaccgcgctc ggcgaaatag gcggcaaagc tcgcccacag ccaggatccg gtcaggacgc
     6721 cgtggacgag gaggagtggc ggtttgcggc tgcgccgctc gggctggtgg gtctcgacat
     6781 ggatgccctc gggctcgatg aagacctcgt cacgcttgat cgcctggacc acggatgctc
     6841 aaccctccag cggatagaag accatgccgg tcagcaattt gggatagaag aacgtcgatt
     6901 tctgcggcat cacgtccccg gcagcggcca ccgcggtcag ttcctccaac cgggtcgggc
     6961 gcaccaggat cgcagccgcc acctcgcccc gggctacacg accgcgggca tcggcctcgc
     7021 ttctagtgta gaggagcatc tccccggcgg ccacgtccgc cgccgagatg ccgagccgat
     7081 cgccgagcac cgccgcgtgc aggagcgcga ggtccagcgt tcgggccggt tcggaccctg
     7141 gttcgcgtcg catccggtcg gccgcggcat ccacgtcgcc gatgaccagc cacccttcgc
     7201 cgtcgggcaa caccagcccg aacacggctt cctcggcgtc gcgaatgtcc gccatccgct
     7261 cccccagcgc ctccggcgcg acggggacgg cctgga
//
LOCUS       DASYGU010000034         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1085778, whole genome shotgun sequence.
ACCESSION   DASYGU010000034 DASYGU010000000
VERSION     DASYGU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085778"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGO32_00860"
     CDS             complement(<1..271)
                     /locus_tag="VGO32_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006295357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7199333.1"
                     /translation="MRDELTPREVALELGVTVRTVQRWIATGRLAGSRVGGRMRVPRS
                     ALAAVTGAEPAAGPREIHALLIANRGEIAARIGRTAERLGIRVIRV"
     gene            complement(305..631)
                     /locus_tag="VGO32_00865"
     CDS             complement(305..631)
                     /locus_tag="VGO32_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199334.1"
                     /translation="MGLILVGSGVYSFVEALFLTQSMQFGLIVGPILVLAGLPGVIAF
                     GLLWREGRGWRLAVGWSAAMLLVAIWSILSVILSPATHGPPPVSVVWAIASAVALVTL
                     LIARTR"
     gene            complement(685..1464)
                     /locus_tag="VGO32_00870"
     CDS             complement(685..1464)
                     /locus_tag="VGO32_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7199335.1"
                     /translation="MERLGPTARVTLDRPEVRNAFNAELIAALTATFVTLGEESPDAL
                     RVVVLAGDGPVFCAGADVAWMRTAASLDVDANEADAGRLATMLETIDTCPVPVVTRVH
                     GAALGGGMGLCAVSDIVVSTADATFGFTETRLGILPAVIGPFALAKIGESHARALFLA
                     GSRFGAERAREIGLVHEVVPDEAAADARVAELVEELLAAGPTAVREAKGLIRRLRGQP
                     APQARALTVAAIARQRTSPEGQEGLSAFLEKRGPAWKDTTR"
     gene            complement(1479..2480)
                     /locus_tag="VGO32_00875"
     CDS             complement(1479..2480)
                     /locus_tag="VGO32_00875"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEV7199336.1"
                     /translation="MAQPLLHRQPLHHRLLQHRPTRRLPRHRQPPHLLHGQTRPLHRQ
                     PLHRKRRRLRRTDPGPPTRRSAPTTNDEERPLPGSHARGVPTGTLEESAVALYEAAVA
                     ARDAEGGDDPAPLRAFYRAIGGATLLLPVPPGTEQEAQAAVDRAVDDEEEVEVGVLLG
                     RGTDGAPVSVAFGSYAALAAWSPLGSTSLALPARIALTNLANAGMPVILDPAGPIPYR
                     FEPDEIAALAAGRMPGADEPLFSANGGSSIRVRLPGPEARDTERRLASELADWPGLHE
                     AYLVEADTGSGWGLLLGLVGGGGPMPPLPEDVEALWLAEPLLGSVRAVSDPFHRGHR"
     gene            complement(2734..>3011)
                     /locus_tag="VGO32_00880"
     CDS             complement(2734..>3011)
                     /locus_tag="VGO32_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199337.1"
                     /translation="NRYLPWRRRRWSELNAADRVIRQSAATRTVEVTDAEVERVLATR
                     ALYRLDYEDLLDQAPDPIGDFVGGRYDRLARAELESVGLRQPSRGDG"
BASE COUNT          455 a         1094 c         1071 g          391 t
ORIGIN      
        1 ggacccggat gacgcgtatc ccgagccgct ccgccgtccg cccgatccgc gccgcgatct
       61 ccccgcggtt ggcgatcagg agcgcgtgga tctcgcgcgg gccggccgcc ggctcggccc
      121 cggtgacggc tgccagcgcg ctgcgcggaa cgcgcattcg gccgccgacc cggctgcctg
      181 ccaggcgccc ggttgcaatc cagcgctgga cggtgcggac cgtgaccccc agctccaggg
      241 caacctctcg aggggtcagc tcgtcgcgca cgtcgcgatt gtcgcagggg tcaactcgag
      301 cttgtcagcg ggtcctcgcg atgagcaggg tgaccaatgc cacggcggac gcgatggccc
      361 agaccacgga aacgggcggt ggaccgtggg tagctggcga caggatcacg ctcaggatgc
      421 tccagatcgc cacgagcaac atggcagcgc tccagcccac cgccagccgc cacccacggc
      481 cctcccgcca gaggagcccg aacgcaatga cgccagggag gccggccagc accaggatgg
      541 gcccgacaat caggccgaat tgcatcgact gcgtgaggaa cagcgcctcg acgaaggaat
      601 agacaccgct gccgacgagg atcaggccca tcagcccgac cagcccggca gccaaccgca
      661 tggagccgga gagcccagtc gcggtcaacg cgtcgtgtcc ttccaggccg gaccgcgctt
      721 ctcgaggaac gcagaaagcc cttcctggcc ctcggggctc gtgcgctgtc gggcgatggc
      781 ggcgacggtc agcgcccggg cctggggggc gggctggcca cgcaaccggc ggatgagtcc
      841 ctttgcctcg cggacggcgg tcggtcccgc ggcgagcagc tcctcgacca gctcggcgac
      901 ccgcgcgtcg gccgccgcct catccggaac gacctcgtgg acgagtccga tctcgcgtgc
      961 ccgctcggct ccaaaccgac tcccggccag gaacagggcg cgggcgtggc tctcgccgat
     1021 cttggccagc gcgaacgggc cgatcaccgc cggcaggatg cccaggcggg tctcggtgaa
     1081 tccgaacgtc gcgtcggcgg tgctgaccac gatgtccgac accgcgcaca ggcccatccc
     1141 gccacccagc gcggcgccat gcacccgggt cacgaccggc acagggcagg tgtcgatggt
     1201 ctcgagcatg gtggccagcc gtccggcatc ggcctcattg gcgtcgacgt cgagactcgc
     1261 cgccgtgcgc atccatgcca cgtcggctcc ggcgcagaac accggcccgt caccggccag
     1321 gaccacgacg cgcagcgcgt ccggggactc ctcgcccaga gtgacgaacg tggccgtcag
     1381 cgcggcgatg agctccgcgt tgaacgcgtt tctgacctcc ggccgatcca gcgtcacgcg
     1441 ggccgtcggc ccgaggcgct ccacccggag cggcgcgctc aacggtggcc gcggtgaaag
     1501 ggatcagaaa cggcccggac gctgccgagc aggggctcgg cgagccacag ggcctcgacg
     1561 tcttcaggca gcggcggcat cggtccgcct ccgccgacca ggcccaggag caacccccag
     1621 ccggagccgg tatcggcctc gacgaggtat gcctcgtgca gccccggcca gtccgcgagc
     1681 tcagacgcca gccggcgctc ggtgtcgcgc gcctcgggac ctggaaggcg gacccggatc
     1741 gagctcccgc cgttcgcgct gaacagcggc tcgtcggccc ccggcatgcg accggcggcc
     1801 agtgccgcga tctcgtcggg ctcgaaccga tacggaatcg gcccggcggg atccaggatg
     1861 accggcatgc cggcgttcgc gaggttggtc aaggcgattc gcgccgggag tgcgagggag
     1921 gtagacccca gcggcgacca ggccgccagc gcggcatagg acccgaatgc cacgctgacc
     1981 ggggcgccgt cggtcccgcg gccgaggagt acgccgacct cgacctcctc ctcgtcatcc
     2041 accgcccggt cgaccgcggc ctgcgcctcc tgctcggtcc cgggcgggac gggcaggagg
     2101 agggtggctc ccccgatcgc gcggtagaag gcgcggagcg gggcgggatc atcgccgccc
     2161 tcggcatcgc gggccgcgac ggcggcctcg tacagcgcga ccgcgctttc ctccagggtc
     2221 ccggtcggga ccccgcgagc gtgggaaccg ggaagcggcc gttcctcgtc gttagtcgtt
     2281 ggtgcgctcc ggcgggtcgg tgggccaggg tcggttctcc gcagccggcg ccgcttccgg
     2341 tgcagcggct ggcggtgcag cggccgtgtc tggccatgca ggagatgcgg aggctgtcga
     2401 tggcgcggca ggcggcgcgt cggccggtgc tggagcaacc ggtggtggag cggctggcgg
     2461 tgcagcaggg gctgcgccaa cagcggctgg agccgccgcg gcactcgcct cggccggctc
     2521 cgcggccttt cggccacgac gctgcgcgcc gaacaggacc gtgatcaggc ctacggcggc
     2581 gacgatgacc ccggcccagg gctgatacgg atccacgatc tccatcagct ggaattggaa
     2641 gcccaggttc ggcaggatga gcgacccgac ccccaggatc acgagcagga ctccgagact
     2701 gatcattggg ctgaccctcc gttttggcgc agtctagcca tcccccctgc tcggctgacg
     2761 caatccgacg ctttccaact cggcccgtgc caggcggtcg tagcggccac cgacgaagtc
     2821 gccgatgggg tccggggcct ggtccagcag gtcctcgtag tccaggcgat acagggctcg
     2881 ggtggccaag actcgctcaa cctccgcgtc cgtgacctcc accgtgcgcg tggctgctga
     2941 ttggcgaatc acccggtcgg cggcgttgag ctcgctccag cgacgacgac gccagggcag
     3001 ataccgattc a
//
LOCUS       DASYGU010000035         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1497060, whole genome shotgun sequence.
ACCESSION   DASYGU010000035 DASYGU010000000
VERSION     DASYGU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497060"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGO32_00885"
     CDS             complement(<1..512)
                     /locus_tag="VGO32_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV7199338.1"
                     /translation="MTTDNPTPPELDPEDRPHGHSVYEPSEFLGAPASEGIPESEVSL
                     AQRFLNVRTIGSLVFGLAMMFLLFRVVLGIDFGTTIAIIQGANPALLLAALVVYYLTF
                     PLRGGRWWYILRKVGTHIRYWVATEILFLSWFVNCLVPAKLGDLYRAFLLKGNYGGSA
                     SRTVGTIFIER"
     gene            complement(509..1267)
                     /locus_tag="VGO32_00890"
     CDS             complement(509..1267)
                     /locus_tag="VGO32_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV7199339.1"
                     /translation="MTAAVAVGAMTRESTGSDSRSLAFADFHCHTRHSPDSRLSEDRF
                     LELAVERGLTHVAVTDHNTIEGSVAVRSRAADLGLDDRLTVILAEEVSTADGEIVALF
                     IESTIPRDLTAEATADAIHEQGGLVSVPHPYDPFRRSHITPDALDRLAATDRIDLIEV
                     FNSRVTLGRHNEDAAEFAARHHLPRVACSDSHTAMEVAMSFNALPAFTTAAELKEVLP
                     ENEWHGNRTTKLVHLGTRWAVASKWLARKTGPDR"
     gene            complement(1264..>2237)
                     /locus_tag="VGO32_00895"
     CDS             complement(1264..>2237)
                     /locus_tag="VGO32_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV7199340.1"
                     /translation="GTANILVYVQGKGVVLNEPSVVAISEDDNRLVAVGEEAREMIGR
                     TPGNVRAIRPMRDGVIADYLITEAMLHYVINKVARVRIFRPDVMISVPSGVTSVEKRA
                     VRDAALKAGARNAFLIEEPLAAAIGANVPISGPAGNMIIDIGGGTTEVAVIALGGIVV
                     SHSVRVGGNRFDEAITSFVRRKYNLMIGERTAEEVKIRIGSALPLDEPLQMEVKGRDL
                     IAGLPRTIPMTSGEVTEAIEQPLQQIISAVRNVLEQTPPELASDIIDKGMVMSGGGSL
                     LRNLDALLTQVTGIPCHVAENALFSVAVGTGRALEQFATFRKSLVSS"
BASE COUNT          357 a          726 c          783 g          371 t
ORIGIN      
        1 cgctcgatga agatggtccc caccgtccgc gacgcggagc cgccgtagtt gcccttcagc
       61 aggaaagcgc ggtagaggtc accgagcttg gccggcacca ggcagttgac gaaccacgac
      121 aggaacagga tctcggtcgc gacccagtac cgaatgtggg taccgacctt gcgcaggatg
      181 taccaccacc tcccgccccg gagcgggaag gtcaggtagt agacgacgag ggcggccagg
      241 aggagggcgg gattcgcgcc ctggatgatc gcgatcgtcg tgccgaagtc gatgcccagg
      301 acgacccgga agagcaggaa catcatggcc agcccgaaga cgagcgagcc aatcgtccgc
      361 acattgagga atcgctgggc cagcgagacc tcgctctcgg ggatcccttc cgatgccgga
      421 gcgccgagga actcggaggg ctcgtacacc gagtgaccgt gtggccggtc ttcggggtcg
      481 agctcgggcg gcgtggggtt gtcggtcgtc atcggtccgg cccggtcttc ctcgcgagcc
      541 acttggaggc cacggcccac cgcgtgccga ggtgcacgag cttggtcgtc cggttgccgt
      601 gccactcgtt ttcgggcagg acctccttga gctcggcggc ggtcgtgaag gcgggcaggg
      661 cgttgaagct catggccacc tccatcgcgg tgtgggagtc cgagcacgcc accctgggca
      721 ggtggtgacg ggcggcgaac tccgccgcgt cctcgttgtg gcggccaagc gtgacccggc
      781 tgttgaagac ctcgatgaga tcgatccggt ccgtggcggc cagccggtcg agcgcgtccg
      841 gtgtgatgtg cgagcggcga aaggggtcgt acgggtgcgg aaccgaaacg agcccaccct
      901 gctcgtggat cgcgtccgcg gtcgcctccg ccgtcaggtc ccgcgggatg gtcgactcga
      961 tgaacagggc cacgatctcg ccgtccgcgg tggacacctc ctcggccagg atcaccgtca
     1021 gccggtcgtc gaggcccagg tcggctgcgc gggaccggac cgcgaccgaa ccttcgatcg
     1081 tgttgtggtc ggtaaccgcg acgtgggtca ggccccgttc gacggcgagc tcgaggaacc
     1141 gatcctcgct gaggcgggaa tcggggctgt ggcgggtatg gcaatggaag tcggcaaagg
     1201 ccaggcttcg actgtcgctg cccgtgctct cccgggtcat ggcgcccacg gcgacggcgg
     1261 cggtcatgag ctcaccaggc tcttgcggaa ggtcgcgaac tgctcgagcg cccggccggt
     1321 gccgaccgcc accgagaaca gcgcgttctc cgcgacgtgg cagggaatcc cggtcacctg
     1381 ggtcagcagc gcgtcgaggt tccggagcag gctgccgccg ccggacatga ccatgccctt
     1441 gtcgatgatg tcgctggcca gctctggcgg ggtttgctcc aggacgttcc gaacggcgga
     1501 gatgatctgc tggagtggct gctcgatggc ctcggtcacc tcgccgctgg tcatcgggat
     1561 ggtgcgtggc aggccggcaa tcaggtcacg gcccttgacc tccatctgga gcggctcgtc
     1621 gagcgggagg gccgagccga tccggatctt cacctcctcc gccgtacgct ccccgatcat
     1681 caggttgtac ttgcgccgga cgaacgaggt gatcgcctcg tcgaagcggt ttccgccgac
     1741 ccgaaccgaa tgcgatacca cgatccctcc cagggcgatg accgccacct cggtcgtgcc
     1801 gccgccgatg tcgatgatca tgttgccggc cggcccgctg atcgggacat tggcgccgat
     1861 ggccgcggca agtggctctt cgatcaggaa cgcgttgcgg gcgcccgctt tcagggccgc
     1921 gtcgcgcacg gcgcgcttct cgacgctggt cacgccggag ggcacgctga tcatgacgtc
     1981 gggccggaag atgcggacgc gggcgacctt gttgatcacg tagtgaagca tggcctcagt
     2041 gatgaggtag tccgcaatga ccccatcgcg catcggccgg atagcccgaa cgttccccgg
     2101 cgtacggcca atcatctccc gggcctcctc gccgacggcc accagtcgat tgtcgtcctc
     2161 cgatattgcc accaccgatg gctcgttcag gacgacgccc ttgccctgga cgtagacaag
     2221 gatgttggcg gtgccga
//
LOCUS       DASYGU010000036         6364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1497189, whole genome shotgun sequence.
ACCESSION   DASYGU010000036 DASYGU010000000
VERSION     DASYGU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497189"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..102
                     /locus_tag="VGO32_00900"
     CDS             19..102
                     /locus_tag="VGO32_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEV7199341.1"
                     /translation="MAGTVSAVHVRPGDQVQRGDLLAEVSA"
     gene            115..1017
                     /locus_tag="VGO32_00905"
     CDS             115..1017
                     /locus_tag="VGO32_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEV7199342.1"
                     /translation="MTHVDVRVYEVGPRDGLQNEAHPIPTETKLGFIAKLADAGLREI
                     EATSFVAPTAIPQLADADELMAALDRRPGVRYPVLVPNSRGLERAIAANADAVCVFTA
                     ASETFTQHNIGMSIAESLAAFEPVVAHAKREGWWIRGYLSTAFGCPYEGAVAERAVVD
                     VAERLLALGVDELSIGDTIGVAGPADVRRVVGALVGAGIGPDRLAMHFHDTRGTAVAN
                     VATAIGLDIRCFDASTGGTGGCPYAPGAAGNLATEDLVYLLDREGLRHGLDLDGILGA
                     ARFIADALGKPLASKVGQAGGWPA"
     gene            1028..1993
                     /locus_tag="VGO32_00910"
     CDS             1028..1993
                     /locus_tag="VGO32_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV7199343.1"
                     /translation="MADAQADRRPVILDVDTGIDDMVALLIAATAPELNLRGVTCVAG
                     NVELQHVARNTGTLLQMVGRGDVPVSIGAARPLTRRLRTAPETHGPTGTGYAELRGEQ
                     RALETTDFTAVPAARYLEAHLTRHPGEMTIVATGPLTNLASAANNGVDLAGGAREVIW
                     MGGALSVRGNTTEHGEWNACVDPEAAEICLSGGAVDRIFPLDATQQVLFTLDDLDRLP
                     DNDIANVVRDALRFYVGYHRVADGIDGCYLHDPVTLVGSLLRPDLVTGSRDRRLACDT
                     NADPAVAGTIRPADDDPRPMLNVVTGINGPAMREEVLERLTRAVA"
     gene            2028..3158
                     /locus_tag="VGO32_00915"
     CDS             2028..3158
                     /locus_tag="VGO32_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV7199344.1"
                     /translation="MTTEEQVEAQASSGWLRVALGVVAFLFTVVAGIAAFGQFGGGTV
                     DPNTGADATAANSFALVLAVAMLVGMTVTLVLERIQTGHFGSLSRQFTLRVIVLMPIA
                     IAINIVLGQTVAAALKIPIYLDSIGTILVGVLAGPIAGAVTGALGNILWAYVIPPPFQ
                     YPPAAAFAVVAAVIGIAAGLAGRYGLMRPRTNRSTGQLVIAGVLVALIIIGLAWLASV
                     GYGLIFDTDINPLPTTDSELFLVLGYIALLLVVAAVLGVFALLFGRRDLTAAYAVVTG
                     VATGIVAALISAPIAAGVFGGVTGAGTDFLVAAFRQAGVDIYAATTGQGLISDPIDKA
                     TTFVIVYLITVAMAVRLKARFPQGEYLIEDTDAEVPAPERTG"
     gene            3194..3958
                     /locus_tag="VGO32_00920"
     CDS             3194..3958
                     /locus_tag="VGO32_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEV7199345.1"
                     /translation="MPPTPTAWHRLNPLTRLVAATVTAVNATLLGGVVGPVGLTLFAV
                     LLPAALAGVLPRLLRVGLILALPLAISVFVVNLFFYPSGSTVLFRIGPITATAEGLAF
                     AIETLVRLLAITGALALFYLTTPIGSLVVDLERRGVSPRLAFVVSASVRTVPAILERA
                     GQIADAQRARGLDTEGSPWRRVRGLVPLVGPVLLGSIGDVEDRAMALEARGFSRPGRR
                     SLLWAPGDRTAERVARWLLALSVPVLIVAGFAGWLP"
     gene            3981..4778
                     /locus_tag="VGO32_00925"
     CDS             3981..4778
                     /locus_tag="VGO32_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199346.1"
                     /translation="MLTLDGVTYRYAGSRRPSLTDISLTLGPGEVVGLLGPNEAGKST
                     TCLVAAGLAPRAVGGSLTGRAEVDGRRLADVPPGEMAGLVGIGFASAGLSGVCSTVYE
                     EVAFGPMNLGLPRAEVMRRTEEALETVAAGHLAERDPEHLSGGQRQLIALAGVLAMQP
                     AHLVLDEPTAMLDPSGTRLVGAAIAALAAGGASILVAEQKTDLLADICTRAVVLAEGR
                     VVLEGSAESVLTDPVLETIGVDPPTRVRLARLATTAGLDPERLAVPA"
     gene            4775..5608
                     /locus_tag="VGO32_00930"
     CDS             4775..5608
                     /locus_tag="VGO32_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199347.1"
                     /translation="MIDLSLDQVVHEYAGGVRALDGLNLRIGAGEAVALVGQNGSGKT
                     TLVRHLNGLLRPTAGKVLVGDRDAAELRVAALARQVGLVFQDPDRQIFAGTVRREVEF
                     GPRNVGLRGPGLDDAVSAALSAVGLEGEQATNPYDLGAARRKLLAIASVLAMQTPVLV
                     LDEPTTGQDRAGVALVQGLVSDVVGTGRTVIAVSHDLRFVAETFPRVVVLRAGRVVLD
                     SSPKEVFAPPNRETLASTGLEPPWAAQLAGRLGLGPVATEAELVAAVARAGAGAGAGA
                     G"
     gene            complement(5605..6042)
                     /locus_tag="VGO32_00935"
     CDS             complement(5605..6042)
                     /locus_tag="VGO32_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199348.1"
                     /translation="MTSAPRSRDDATRAALRETRIVANRVSFSSGTRREVNRALDTLE
                     SQLGRNQVNATDTARALELLNRAHPSLLFGLLRDRAVAERYGAALRELGLRGVGQRLD
                     EVPHSVMAVPIPGPTNGRRHRDELPAEERVDAVGRALPPPRAP"
     gene            6043..>6364
                     /locus_tag="VGO32_00940"
     CDS             6043..>6364
                     /locus_tag="VGO32_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199349.1"
                     /translation="MGILRAEGDWQALVARALAAVERPIPVDPRFVPRRAVPDPALAA
                     EDARRFDRSRFPTARDAATLLLIYPAEAELVIPLTVRHAALASHPGEISLPGGAVEEG
                     DASLE"
BASE COUNT          828 a         2274 c         2302 g          960 t
ORIGIN      
        1 cacgcgatca gcgcgccatt ggccggcacg gtcagcgccg tccatgtccg gcccggcgac
       61 caggtccagc gcggcgacct cctggccgag gtgtcagcgt aggatgcggc ggccatgacc
      121 cacgtcgacg tccgggtcta cgaggtcggc ccgcgagacg ggctgcagaa cgaggcccac
      181 ccgatcccga ccgagacgaa gctcggcttc atcgcgaagc tcgccgacgc gggcctgcgc
      241 gagatcgagg cgacctcgtt cgtggcgccg accgccattc cgcagctggc cgatgccgac
      301 gagctgatgg ccgccctcga tcgacgcccg ggggtccgct acccggtcct cgtccccaat
      361 tcgcgcggcc tggagcgcgc gatcgcggcc aacgccgacg ccgtctgtgt cttcaccgca
      421 gcctcagaga cgttcaccca gcacaacatc gggatgtcga tcgccgaatc actggccgcc
      481 ttcgagccgg tcgtggccca cgccaagcgc gaggggtggt ggatccgggg ctacctatcc
      541 accgccttcg gctgcccgta cgagggagcg gtcgccgagc gggcggtggt ggacgtggct
      601 gagcggctgc tggccctagg cgtggacgag ctgagcatcg gtgacacgat cggggtggcc
      661 ggaccggccg acgtccgtcg ggtggtcggc gccctggtcg gtgccggcat cggtcccgac
      721 cggctggcga tgcacttcca tgacacgcgc ggcacggcgg tcgccaacgt ggcgaccgcc
      781 atcggcctgg acatccgctg cttcgacgcc agcaccgggg gcaccggcgg ctgcccgtac
      841 gctcccggcg cagccggcaa cctggcgacc gaggacctcg tgtacctgct cgaccgggag
      901 gggctccgcc atgggctcga cctggacggg atcctgggcg cagcccggtt catcgcggat
      961 gcgctgggca agccgctcgc ttccaaggtg ggtcaggccg gcggctggcc agcgtaggat
     1021 gcgaccgatg gcggacgccc aggccgatcg gcgaccggtg atcctcgacg tcgacaccgg
     1081 gatcgacgac atggtggccc tgctcatcgc cgccaccgcc cccgagctga acctccgcgg
     1141 cgtgacctgc gtggccggca acgtcgagct ccagcacgtg gcccgtaaca ccgggaccct
     1201 gctccagatg gttggtcgcg gggacgtacc ggtgtcgatc ggcgcggcgc ggccgctcac
     1261 ccgtcgcctg cggaccgccc ccgagacgca tggccccacc ggcaccggct atgccgagct
     1321 gcggggcgag cagcgggcac tggagacgac cgacttcacc gccgtgcccg ccgcccgcta
     1381 cctcgaggcg catctcaccc gccacccggg ggagatgaca atcgtggcca ccggaccact
     1441 gaccaacctc gcctccgccg cgaacaatgg ggtggacctc gccggtggag cccgcgaggt
     1501 gatctggatg ggcggcgcgc tgtccgtccg gggcaacacc accgagcacg gcgaatggaa
     1561 cgcatgcgtc gaccccgagg cggccgagat ctgcctgtcg ggcggcgccg tggaccgcat
     1621 ctttccgctc gacgcgaccc agcaggtctt gttcaccctg gacgacctcg accgcctgcc
     1681 ggacaacgac atcgccaacg tcgtgcgcga cgcgctccgc ttctacgtgg gctatcaccg
     1741 ggtcgcggac gggatcgacg gctgctacct gcacgatccg gtgaccctgg tcggttcgct
     1801 gctccgtccc gaccttgtca cgggcagccg cgaccgccgg ctggcgtgcg acacgaacgc
     1861 ggacccggcc gtggccggca cgattcggcc cgcggacgac gatccgcggc cgatgctgaa
     1921 cgtcgtcacc ggtatcaacg gaccggcgat gcgcgaggag gttctcgagc gcctgacgcg
     1981 ggcggtcgcc tgaccccgcc gccgctgaac caaaggagac ggaaccgatg acaaccgagg
     2041 aacaggtgga ggcacaggcc agctcgggct ggctccgggt agcgctgggg gtggtcgcat
     2101 tcctgttcac ggtcgtggcc ggcatcgcgg ccttcggcca gttcggcggg ggcacggtcg
     2161 acccgaacac cggagccgat gcgacggcgg cgaactcgtt cgctctcgtc ctcgccgtgg
     2221 ccatgctggt cgggatgacg gtgaccctgg tcctggaacg gatccagacc gggcacttcg
     2281 gatcgctttc gcggcagttc accctccggg tcatcgtcct catgccgatc gcgatcgcga
     2341 tcaacatcgt gctcggccag accgtggccg cggcgctgaa gatcccgatc tacctcgact
     2401 cgatcggcac gatcctggtc ggggtcctgg caggaccaat cgccggcgcc gtgaccggcg
     2461 ccctgggcaa catcctgtgg gcgtacgtca ttccgccgcc gttccaatac ccgccggcgg
     2521 cggcgttcgc ggtcgtggcg gcggtgatcg ggatcgcggc cggccttgcc ggtcggtacg
     2581 gactcatgcg cccccgcacg aatcggtcca ccggccagct cgtgatcgcc ggcgtgctcg
     2641 tcgcgctcat catcatcggc ctggcctggc ttgcatcggt cggctacggg ctgatcttcg
     2701 acacggacat caatccgctg cccaccacgg acagcgagct gttcctggtc ctgggctaca
     2761 tcgcgctcct gctcgtggtc gcggccgtgc tcggcgtctt cgcgctactc ttcgggcgac
     2821 gcgacctgac ggcggcgtac gcggtcgtga ccggggtggc aaccggcatc gtggccgcgc
     2881 tgatcagcgc cccgatcgcg gccggcgtgt tcggcggcgt caccggcgcc ggtaccgact
     2941 tcctggtggc cgcgttccgc caggccgggg tcgacattta cgccgcgacc accggtcagg
     3001 ggctgatcag cgatccgatc gacaaggcga cgaccttcgt gatcgtgtac ctcatcacgg
     3061 tcgcgatggc cgtccggctg aaggcccgat tcccacaggg cgagtacctc atcgaggaca
     3121 ccgacgccga ggtgcccgcg ccggagcgca cgggctgacc gaggccgccg agccggcggc
     3181 gccggccttc ctgatcccgc cgacgcccac cgcctggcat cggctcaacc cgctgacccg
     3241 cctcgtggcg gccaccgtca ccgcggtcaa cgcgaccctg ctcggtgggg tcgtcggtcc
     3301 tgtgggcctc accctgttcg cggtccttct acccgctgcg ctggccggcg tcctgccccg
     3361 cctgctcagg gtggggctga tcctggccct ccccctcgcg atcagcgtgt tcgtggtcaa
     3421 cctgttcttc tacccgtccg gctccaccgt gctgttccgg atcggtccga tcacggccac
     3481 cgccgagggt ctcgcgtttg cgatcgagac cctggtccgc ctcctggcga tcaccggtgc
     3541 tttggccctg ttctacctga ccaccccgat tggctccctg gtcgtggacc tcgagcggcg
     3601 gggggtgagc ccgcggctgg cgttcgtggt cagcgcctcg gtgcggaccg tgcctgccat
     3661 cctggaacgg gcggggcaga tcgccgatgc tcagcgagcc cgcggcctgg acacggaagg
     3721 cagcccgtgg cggcgggtcc gcggcctcgt gccgttggtc gggccggtgc tgctggggtc
     3781 gatcggcgat gtggaggacc gggccatggc cctcgaggcc cgcggcttct cccgccccgg
     3841 ccgccgctcc ctgctgtggg cacctggcga ccggaccgcc gagcgggtcg cgcgctggct
     3901 gctcgcgctg tcggttccgg tcctgatcgt ggccgggttc gccgggtggc tgccatgact
     3961 gttcgtctta ggtggctccc atgctgaccc tggacggggt cacctaccgc tacgccggca
     4021 gccgtcgccc gtcgctgacc gatatctcgc tgacgctcgg gccgggcgag gtcgtgggcc
     4081 tgctcggccc caatgaggcg ggcaaatcca cgacctgcct ggtcgcggcc ggcctcgcgc
     4141 cgcgggcggt gggcggctcc ctgaccggcc gcgccgaggt cgatggccgg cgcctggcag
     4201 acgtgccgcc gggtgagatg gccggtctgg tcggcatcgg cttcgcgtcc gccggcctga
     4261 gcggggtctg ttcaacggtc tacgaggagg tcgccttcgg accgatgaac ctgggcctgc
     4321 cgcgggccga ggtcatgcgc cgcacggagg aggccctcga gaccgtggcc gccgggcacc
     4381 tcgccgaacg cgaccccgag cacctgtccg gcggacagcg ccagctcatc gcgctggcgg
     4441 gcgttctggc catgcaaccg gcgcacctgg tcctggacga gcccaccgcc atgctcgatc
     4501 cgagcgggac gcggctcgtc ggtgcggcga tcgctgccct ggcggcgggc ggggcatcga
     4561 tccttgtagc cgaacagaag accgatctgc tggccgacat ctgcacgcgg gccgtggtcc
     4621 tggccgaagg ccgggtggtg ctggagggct cagcggagtc ggtactgacc gatcccgtgc
     4681 tcgaaacgat cggcgtggat ccgccgacgc gagtgcggct cgcgcggctg gccacgaccg
     4741 ccgggctcga ccccgaacga ctggcggtgc cggcatgatc gatctcagcc tcgaccaggt
     4801 cgtccacgag tacgccggcg gcgtgcgagc cctcgacggg ctcaacctgc ggatcggcgc
     4861 gggcgaggcg gtggccctgg tcggtcagaa cgggtcgggc aagacgacgc tcgtccgcca
     4921 cctcaatggg ctgctccggc ctaccgccgg caaggtgctg gttggtgacc gggatgccgc
     4981 cgagctgcgt gtggcagccc tcgcgcgcca ggtgggactc gtgttccagg atccggaccg
     5041 ccagatcttc gccgggacgg tgcgccgcga ggtcgagttc gggccacgga acgtgggact
     5101 gcggggtccg gggctggacg acgccgtgag cgcggcgctg tccgcggtcg gcctcgaagg
     5161 cgagcaggcg acaaacccgt acgacctggg tgccgcgcgt cgaaagctgc tggccatcgc
     5221 gtcggtgctg gccatgcaga cgccggtgct cgtcctggac gagccgacaa ccgggcagga
     5281 ccgggccggg gtggccctcg ttcaggggct cgtgtcggac gtcgtcggga cgggccggac
     5341 ggtcatcgcg gtcagccatg acctgcggtt tgtggccgaa accttcccgc gggtggtcgt
     5401 cctgcgcgcg ggacgcgttg tcctcgacag ttcaccgaaa gaggtcttcg ccccgccgaa
     5461 ccgggagacg ctggccagca ccggtctcga gccgccgtgg gcggcgcagc ttgcggggcg
     5521 gctgggactc ggacccgtgg cgacggaggc agaactcgtg gcggcggtgg cgagagcggg
     5581 agcgggagcg ggagcggggg cgggctaggg tgcgcggggc ggcgggagcg cccggccgac
     5641 cgcgtccacc cgttcctcgg ccggaagctc gtctcgatgt cggcggccgt tcgtgggccc
     5701 cggaatcggc acggccatca ccgaatgggg cacttcgtcg aggcgctgcc ctacaccgcg
     5761 caggcccagc tcgcggagcg cggccccata gcgctcggcc acggcccggt ctcggagcag
     5821 gccgaacagc agggatggat gtgcccggtt cagaagctcg agggcgcgcg cggtatcggt
     5881 cgcattcacc tggttccggc caagctgcga ctcgagcgta tcgagcgccc ggttcacctc
     5941 gcgacgagtg ccgctgctga agctcacccg gttggccaca atgcgggtct cgcgcagggc
     6001 ggcccgggtg gcgtcgtctc ggctgcgcgg cgcggaggtc atatgggcat cttacgggct
     6061 gagggcgact ggcaggccct ggtggctcgt gcgctggcgg ccgtcgagcg gcccatcccg
     6121 gtggacccgc ggttcgtgcc ccgccgtgcg gtgccggacc ccgcgctcgc cgccgaggac
     6181 gcgcggcgct tcgaccgatc ccggttcccg accgcccggg atgccgccac gttgctcctc
     6241 atctaccccg ccgaggcgga gctggtcatc ccgctcaccg tgcggcacgc cgcgctggcc
     6301 tcccatccag gggagatcag cctcccgggt ggagccgtcg aggagggcga cgcgagcctg
     6361 gagg
//
LOCUS       DASYGU010000037         5805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_571693, whole genome shotgun sequence.
ACCESSION   DASYGU010000037 DASYGU010000000
VERSION     DASYGU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571693"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VGO32_00945"
     CDS             <1..523
                     /locus_tag="VGO32_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEV7199350.1"
                     /translation="GTADRLAAMGPEHGFEVVTIDPELDDGPISSTRIREALVAGDVV
                     IATRLLGRWPALNGIVVRGDGRGRELGFPTANLRFDYHPVVPALGIYAGVANGIGALV
                     SIGRRPVFHTDGAVVVEANLLDWDGDLYDKPLRVELVARLRDEQPFASIDALVEQMHR
                     DEGEARAALISVL"
     gene            563..832
                     /gene="rpsO"
                     /locus_tag="VGO32_00950"
     CDS             563..832
                     /gene="rpsO"
                     /locus_tag="VGO32_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV7199351.1"
                     /translation="MPLDPATKVAVANDFATHDGDTGSPEVQIALLTERIKSLTDHLR
                     SYPNDNASRRGLLKLVGQRRRLLAYLVRTDVDRYRTVIARLGLRR"
     gene            1010..3604
                     /gene="pnp"
                     /locus_tag="VGO32_00955"
     CDS             1010..3604
                     /gene="pnp"
                     /locus_tag="VGO32_00955"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV7199352.1"
                     /translation="METGKLAEQADGSVLLRYGDTVVLATAVASEPREGIDFFPLTVD
                     YEERMYAAGKIPGGFIKRESRPSEAAILAMRLTDRPIRPLFPKGYKNDVQVVLTVLSA
                     DQENDPDILAVNGASAALSVSGIPFLGPVGAVRVGRINGEFVANPTTSQLEESEMDLV
                     VAGTRDAIMMVEAGAKILPETVMLDAITWGHNELQKSIELQEKLVTNSGVQKRTPFLG
                     PKADSIVKLGKALSKPGTEFVILDVETTAMKPENGYIVDIAALKVRDGAVVDRFESLV
                     NPGRSIIGHQVHGLTDEDVSSAQTAAEVLPKFVAWAGNAPVVAHNVGFDLPFVLRHLP
                     NDVSWEPNAVFDTLELAYQLYPDAGGYKLADLMRFIFGKDHAAAHRAMPDAEATAELF
                     TSLTGNLVERLDAIRADIAAEVRRGKEAYNRAEQGQALEDVRRRHGIGSALMDVLTKA
                     SVRELVLSENIRIDGRDTTTIRPISVEVGVLPRTHGSGLFTRGQTQALSIATLGPSSD
                     VQRLDTISPETEKRYMHHYNFPPYSVGESRPMRGPGRREIGHGALAERALVPVLPPVD
                     VFPYVIRVVSEVVSSNGSTSMASTCGSTLALMDAGVPISAPVAGAAMGLITDPDTNRY
                     AVLTDITGKEDAYGDMDFKVAGTAEGVTALQMDIKVAGITPEILRDALEQARTARLFI
                     LDKMTAVISASRSELSPYAPRITTIKIPVDKIRDVIGAGGKVIRQITAETGTQINVED
                     DGTIQIAAVDGEAAEKAVRWIEGLTRDVEVGKEYLGKVTRIMNFGAFVEILPGKEGLV
                     HISQLADYRVARVEDVVTIGDELMVVVTEIDRMGRVNLSRRAAMERHMAHSEAPAGAA
                     VGGDAERD"
     gene            3620..4309
                     /locus_tag="VGO32_00960"
     CDS             3620..4309
                     /locus_tag="VGO32_00960"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV7199353.1"
                     /translation="MSGEADLAAIAVEVRACTRCPLAGGRTMAVPGEGNTLSDVLLVG
                     EGPGQREDATGRPFVGAAGQLLEEMLRSIGWERQDVFITNVVKCRPPGNRDPEPIEIS
                     ACSPYLDRQEQALDPVLIVTLGRFSLQRYLPGARISEAHGRLRRSGSRFVLPMYHPAA
                     ALRSTGLRETLARDFRALPTALLEAREAISAEQADPESSQSEPMGPGDAVGPGDTSLP
                     THDDDQQMTLF"
     gene            4319..>5805
                     /locus_tag="VGO32_00965"
     CDS             4319..>5805
                     /locus_tag="VGO32_00965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV7199354.1"
                     /translation="MAGTLRIIPLGGVGEVGKNITVIEADDEIVVVDCGLAFPDPDML
                     GIDLVVPDPTYLAERRDAVRGIILTHGHEDHTGGLPYVLPRISGTPIYASKLTIGLVT
                     GKLKEHKLMGATTLNVVEPGVEFAVGRFRVIPFRVNHSIPGGVGFVIRHERGTVIHTG
                     DFKFDHTPPDGTRADLGLIAEWGKIGVDFLLSDSTRAEREGYTLSEATVGESLRQLIG
                     AAEGRVIMATFASNIGRVQQAIDAAAEHGRKVVLIGRSMEQNSAIAIELGYLDGRGIL
                     VKKDQLERLKPEDLMVVTTGSQGEPMSGLTRMSNRDHRNITIVPGDTVILSASPIPGN
                     EEAVARTIDNLFKEGAMVHYEPMTHCHVSGHGSREELKLMLGLAKPRHFIPVHGEYRM
                     LVQHALLAEGAGVAVDRIFVLQNGQVVEFDGERGRLAGSVQAGAVLVDGLSAVDGVDG
                     VILRDRRALAMDGVVTVALTVASATGRPVSGPDLTGRGFLSDPDDP"
BASE COUNT          964 a         2033 c         1940 g          868 t
ORIGIN      
        1 cggaactgcc gatcgcctgg ccgccatggg tccggagcac ggcttcgagg tggtcacgat
       61 tgaccccgag ctcgacgatg gcccgatctc gtcgacccgg atccgcgagg cgctcgttgc
      121 cggcgacgtg gtcatcgcca cccgcctcct tgggcgttgg ccggctctca acgggatcgt
      181 cgtccgcggc gacggtcgag gccgcgagct cggctttccc accgccaacc tgcgattcga
      241 ctaccacccg gtcgtgcccg cgctgggcat ctacgccggc gtggcgaacg gcatcggcgc
      301 cctggtcagc atcggcaggc ggccggtctt ccatacagat ggtgccgtcg tggtcgaggc
      361 caacctgctc gactgggatg gcgacctgta cgacaaaccg ctgcgcgtgg agctggttgc
      421 gcgtcttcgc gacgagcagc cgtttgcatc aatcgacgca ttggtggagc agatgcatcg
      481 cgacgaggga gaggcgcggg ccgcgctgat ctcggtgctc tgatacgatg cgcacgttca
      541 tgagtttgaa atggaggaat gagtgccgtt agacccggcc acgaaggtcg ctgtcgccaa
      601 tgatttcgcc acccacgacg gcgacaccgg ttcgcccgag gtgcagatcg cgctcctgac
      661 cgagcggatc aagtccctga cggaccatct gcgcagctat cccaacgaca atgcctcgcg
      721 ccgcgggctt ctgaagctcg tcgggcagcg ccgccgactc ctggcatacc tcgtccgcac
      781 ggacgtggat cgctaccgga cggtcatcgc tcgcctcggc ctgcgtcggt aacgtcacca
      841 acccactgac ccggctcagc cgggtttcga ctcgttcgcg tccaccgcga cgcaccccat
      901 ccgagccgcg ccaccctgat tggattggcc gcgccggaag gggcccacac actggaggaa
      961 cctcttggct atcaaggtag aaactgaaat cggcgggcag accctgtcca tggagacggg
     1021 caagctcgcc gagcaggccg atggatcggt cctgctccgc tacggcgaca cggtcgtcct
     1081 ggccaccgcc gtcgcatccg agccccgcga ggggatcgac ttcttcccgc tcaccgtgga
     1141 ttacgaggag cggatgtacg ccgcgggcaa gatccccggc ggattcatca agcgcgagtc
     1201 gcgcccgtcg gaggcggcca tcctggccat gcgcctcacc gaccgcccga ttcgccccct
     1261 gttcccgaag ggctacaaga acgacgtcca ggtcgtcctg accgtcctca gcgcggatca
     1321 ggagaacgat cccgacatcc tggccgtgaa tggtgcctcc gccgcgctga gcgtgagcgg
     1381 gatcccgttc ctgggccccg tcggcgcggt ccgcgtcggc cggatcaacg gcgagttcgt
     1441 ggccaacccg accaccagcc agctcgaaga gagtgagatg gacctggtcg tggccggcac
     1501 ccgcgacgcg atcatgatgg tcgaggccgg ggccaagatc ctgcccgaaa cggtcatgct
     1561 cgacgcgatc acctggggcc acaacgagct ccagaagtcg atcgagctgc aggagaagct
     1621 ggtcaccaac tccggtgtcc agaagcggac cccgttcctc ggccccaagg ccgattccat
     1681 cgtcaagctg gggaaggcgc tcagcaagcc cgggaccgag ttcgtgatcc tcgacgtcga
     1741 gaccactgcg atgaagccgg agaacggcta catcgtggac atcgccgccc tgaaggtgcg
     1801 cgatggcgcg gtcgtggacc gcttcgagtc actcgtcaac cccggtcgct cgatcattgg
     1861 tcaccaggtc cacggtttga ccgatgagga cgtctcctcg gcccagaccg cggccgaagt
     1921 gcttcccaag ttcgtcgcct gggcgggcaa cgccccggtc gtggcccaca acgtcgggtt
     1981 cgacctgccg ttcgtgctcc gtcacctccc gaacgacgtg agctgggagc cgaacgcggt
     2041 cttcgacacc ctcgagctgg cataccagct ctatccggat gccggcggct acaagctcgc
     2101 cgacctcatg cgattcatct tcggcaagga ccacgccgcc gcccaccggg ccatgcccga
     2161 cgcggaggcc accgccgagc tgttcaccag cctgaccggc aacctggtcg agcggctgga
     2221 cgccatccgg gccgacatcg ccgccgaggt gcggcgcggc aaggaggcct acaaccgggc
     2281 cgagcagggc caggccctgg aggacgtccg gcgccgccat ggcatcggct ccgccctcat
     2341 ggacgtcctg accaaggcca gtgtccggga gctcgtcctg tccgagaaca tccggatcga
     2401 cggccgcgac acgacgacca tccgaccgat ctcggtcgag gtcggggtcc tgccccggac
     2461 tcacggctcc ggcctgttca cccgtggcca gacccaggcc ctgtcgattg ccaccctcgg
     2521 cccgtcgagc gacgtccagc gactggacac gatctcgccc gagaccgaga agcggtacat
     2581 gcaccactac aacttcccgc cctactcggt gggggagtct cgaccgatgc ggggccccgg
     2641 ccgccgcgag atcggccacg gtgcactcgc ggagcgcgcc ctcgtgccgg tcctaccccc
     2701 ggtggacgtg ttcccgtatg tcatccgcgt cgtcagcgag gtcgtctcgt ccaacggctc
     2761 gacctcgatg gccagcacgt gtggcagcac cctcgctctg atggacgctg gtgtgccgat
     2821 ctcggccccc gtggccggcg cggcgatggg cctcatcacc gaccccgaca ccaaccgcta
     2881 cgcggtcctg accgacatca ccggcaagga agacgcgtac ggcgacatgg acttcaaggt
     2941 ggccggcacc gccgagggcg tgaccgcgct ccagatggac atcaaggtgg ccggcatcac
     3001 gccggagatc ctccgcgacg ccctggagca ggcccgcacc gcgcggctgt tcatcctgga
     3061 caagatgacc gcggtcatca gtgccagccg ctccgagctg agcccgtatg caccacggat
     3121 cacgaccatc aagatcccgg tcgacaagat ccgcgacgtc atcggcgccg gcggcaaggt
     3181 gatccgccag atcaccgccg agaccggcac ccagatcaac gtcgaggacg acggcacgat
     3241 ccagattgcc gccgtggacg gcgaagcggc cgagaaggcc gtccgctgga tcgagggcct
     3301 gacccgcgac gtggaggtcg gcaaggagta tctcggcaag gtcacccgga tcatgaactt
     3361 cggggccttc gtcgagatcc tgcccggcaa ggaggggctg gtccacatca gccagctcgc
     3421 cgactaccgg gtggctcggg tcgaggacgt cgtgaccatc ggcgacgagc tgatggtggt
     3481 cgtgaccgag atcgaccgca tgggccgcgt caacctgagt cgtcgggccg cgatggagcg
     3541 gcacatggcg cactcggaag ccccggccgg cgctgccgtc ggtggcgacg cggagcgcga
     3601 ctaggccccg gcgcccccga tgagcgggga ggccgatctc gccgcgatcg cggtcgaggt
     3661 gcgggcctgt acccgctgtc ccctggccgg cggccgcacg atggccgtcc ccggggaagg
     3721 gaatacgctg agcgacgtgc tgctggtcgg ggagggaccc ggccagcggg aagacgccac
     3781 cgggcgccca ttcgtgggcg ccgcggggca gctcctggag gagatgctgc gaagcatcgg
     3841 ctgggagcgg caggacgtgt tcatcacgaa tgtggtgaag tgccggccgc cgggcaaccg
     3901 tgacccggag ccgatcgaga tcagtgcctg cagcccgtac ctggaccgcc aggagcaggc
     3961 gctggacccg gtcctgatcg tgaccctggg ccggttctcg ctccagcgct acctgcccgg
     4021 ggcgcgcatc tcggaggctc acggccggct ccgccgctca ggcagccggt ttgtgctgcc
     4081 gatgtaccat cccgctgccg cgctgcggtc cacgggcctg cgcgagacgc tggcgcgaga
     4141 cttccgggca ctgcccaccg cgctgctcga ggcccgcgag gccatctcgg cggaacaggc
     4201 cgatcccgag tcgtcacaat ccgagccgat ggggccgggc gacgcggtcg gaccgggtga
     4261 cacgtcgctc ccgacgcatg acgacgatca acaaatgacg cttttctgaa aggacctgat
     4321 ggcaggaacc ctccgaatca ttccgctggg cggtgtcggc gaagtcggca agaacatcac
     4381 cgtgatcgag gccgacgacg agatcgtcgt cgtcgactgc ggcctggcgt ttcccgaccc
     4441 cgacatgctc ggcatcgacc tggtcgtgcc cgacccgacc tacctcgccg aacggcggga
     4501 cgccgtgcgc gggatcatcc tgacccacgg ccacgaggac cacaccggcg gcctgcccta
     4561 cgtcctgccc cggatcagcg ggacgccgat ctatgcctcc aagctgacca tcggcctggt
     4621 caccggcaag ctcaaggagc acaagctcat gggcgcgacc accctgaacg tggtcgagcc
     4681 gggggtcgag ttcgcggtcg gccggttccg ggtgatcccg ttccgggtca accactcgat
     4741 cccgggcggc gtgggcttcg tcatccgcca cgagcgcggg accgtgatcc acaccggtga
     4801 cttcaagttc gaccacaccc cgccggacgg cacccgggcc gacctggggc tcatcgccga
     4861 gtgggggaag atcggcgtgg acttcctcct gtccgactcg acccgtgccg agcgagaggg
     4921 ctacaccctg tcggaggcca cggtggggga gtccctccgc cagctgatcg gcgcggcgga
     4981 aggccgggtg atcatggcca ccttcgcgtc caacatcggt cgcgtccagc aggccatcga
     5041 cgccgcggcg gagcacggcc gaaaggtcgt gctcatcggt cggagcatgg agcagaactc
     5101 ggcgatcgcg atcgagctgg gctatctcga cgggcgcggc atcctcgtga agaaggacca
     5161 gctcgagcga ctcaagcctg aagacctgat ggtggtgacc accggctcgc agggtgagcc
     5221 gatgtcgggc ctgacccgga tgagcaaccg cgaccatcgg aacatcacga tcgtgcccgg
     5281 ggacacggtc atcctctccg cctcgcccat ccccggcaat gaggaggccg tggcccgcac
     5341 catcgacaac ctgttcaagg aaggagcgat ggtccactac gagccgatga cccactgtca
     5401 tgtctcgggc cacgggtcgc gcgaggagct caagctcatg ctcggccttg ccaagccgcg
     5461 ccacttcatc ccggtccacg gcgagtaccg gatgctcgtc cagcacgcgc tgctggccga
     5521 gggagcgggc gttgccgtcg accgcatctt cgtcctgcag aatgggcaag tggtcgagtt
     5581 cgacggggag cggggtcggc tggcgggcag cgtccaggcc ggcgccgtgc tggtggacgg
     5641 gctgtcggcg gtggacggtg tggacggggt catcctccgc gaccggcgcg cgctggccat
     5701 ggacggggtc gtgaccgtgg ccctgaccgt cgcgtccgcc accggccgtc cggtgagcgg
     5761 ccccgacctc accggccgtg gcttcctgtc cgacccggac gaccc
//
LOCUS       DASYGU010000038         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_262966, whole genome shotgun sequence.
ACCESSION   DASYGU010000038 DASYGU010000000
VERSION     DASYGU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262966"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VGO32_00970"
     CDS             complement(<1..413)
                     /locus_tag="VGO32_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7199355.1"
                     /translation="MTARDRIRNFSIVAHIDHGKSTLADRLLEATHTIDPRQMTSQVL
                     DSMDLEREKGITIKARAVRLLYTAADGEEYILNLIDTPGHVDFSYEVSRSLQACEGAV
                     LVIDASQGIEAQTLANAQLAMEQGLVIIPVINKVDL"
     gene            complement(466..2319)
                     /locus_tag="VGO32_00975"
     CDS             complement(466..2319)
                     /locus_tag="VGO32_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV7199356.1"
                     /translation="MRTPAVATPGAEVTPDAELLPRVRPLPRTAVGAAVAALVFAAML
                     AIPTLAAAGMGVVFDGRVMPRVSVAGVDVGLLTAAEAGERLRSELPDLTAGELVITVD
                     GVSTTVPLSRVDRAHDFDGLVAAAMAVGRRGDAWNDGQTRLATLVVGANVDDAVALDS
                     GQLDRLVIETATGAFVAPVDATVVTNPDGTYSIQPPAAGHRIHPDQIRAALSAALLQP
                     AVSNPAVTFDSEPIPFVVTQREAAEAAAAARAMFAAPLALTDGDDAYPVAPADIQAAI
                     SFGTRSDERYGVILDRTALSAAAATLGAAVRRAPTDAGFTFGAAGPTAVKPAIRGRDL
                     NVPVTVNAIVAGLEQRGAGIEVPGVALSASFVEPTLTTAAAQEILPKLIRLSTWTTYY
                     VPAAGNGWGNNISIPARDLDGMVILPGEDFDFWRDIGPVTFERGYSYGGAIIGGRSTG
                     DQAIGGGICSTSTTLFNTALRAGLEMNSRANHYYYIDRYPMGLDATVFATDYFSQTMS
                     FTNDTESPLIIKSYASPGVVRFDLWGMPLNRTVSFTNPVTWNPTFGHDTVEYTSALPK
                     GVSQRVEYIHNGFSVSVTRKVTDNTTGEVIHDDTYVSHYQTVNGVVQVGTR"
     gene            2444..2722
                     /gene="rpsT"
                     /locus_tag="VGO32_00980"
                     /pseudo
     CDS             2444..2722
                     /gene="rpsT"
                     /locus_tag="VGO32_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
     gene            complement(2968..>4123)
                     /gene="eno"
                     /locus_tag="VGO32_00985"
     CDS             complement(2968..>4123)
                     /gene="eno"
                     /locus_tag="VGO32_00985"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV7199357.1"
                     /translation="GTHEAVELRDGDASRFGGKGVLRAVANVRGEIAGAVTGRDATDQ
                     PGLDQALIDLDGTPNKARLGANAVLGVSLATAHAAAAAAGQPLYRYLGGERAATLPVP
                     MVNILNGGRHAAGGTDFQEFMVVPLGAATFSQGIRWTAEIFHALAALLRARGLPTAVG
                     DEGGYGPALPSNEAAIELVTEAIGRAGYRAGEQVAIALDPAASELFGNGTYALASEGR
                     TLDASGMIDLWADWTDRYAIVSLEDGLAEDDWDGWTALTERLGATVQLVGDDLLVTNQ
                     ARLERAVAEQAANAILVKVNQVGTLTEAIDAVRTAEAAGWGAVVSHRSGETEDTTIAD
                     LAVAMGMGQIKTGSMSRSERTAKYNRLLRIEEELGERAVYAGRAPFRRAG"
BASE COUNT          610 a         1454 c         1445 g          614 t
ORIGIN      
        1 aggtcgacct tgttgatgac cgggatgatg accaggccct gctccatggc cagctgggca
       61 ttggccaggg tctgggcctc gatcccctgg ctggcatcga tgaccagcac cgcgccctcg
      121 caggcctgga gggaccggct gacctcgtag gagaagtcga catggccggg cgtgtcgatc
      181 aggttgagga tgtactcctc gccatcggcg gcggtgtaca gcaggcgaac ggcccgggcc
      241 ttgatcgtga tccccttctc gcgctcgagg tccatcgagt ccaggacctg gctcgtcatt
      301 tggcgcgggt cgatggtgtg agtcgcctcg aggagtcgat cggcgagcgt gctcttgccg
      361 tggtcgatgt gagccacgat cgagaagttg cggatgcggt cgcgggcggt cacggcgggc
      421 aagtctagcc cggcgtccct atcggtctgg ttgccggcgg gcgggtcagc gggttccgac
      481 ctggaccacc ccgttcacgg tctggtagtg gctgacgtac gtgtcgtcgt gaatgacctc
      541 gccggtcgtg ttgtcagtca ccttgcgggt gacgctcacg ctgaagccgt tgtggatgta
      601 ctcgacccgc tggctcacgc ccttgggcag ggctgaggtg tactcgaccg tgtcgtggcc
      661 gaaggtcggg ttccaggtga ccgggttcgt gaagctcacc gttcggttga gcggcattcc
      721 ccacaagtcg aagcgaacga cgcccggcga ggcgtaggac ttgatgatga gcggcgattc
      781 ggtgtcattc gtgaagctca tcgtctggct gaagtagtcg gtcgcgaaca cggtcgcatc
      841 caggcccatg gggtagcggt cgatgtagta gtagtggttc gcccgtgagt tcatctcgag
      901 ccccgctcgc agagcggtgt tgaacagggt ggtggatgtc gagcagatcc cgccgccgat
      961 cgcctggtca ccggtgctcc gcccgccgat gatcgcccca ccgtacgagt agccccgctc
     1021 gaacgtgacc ggaccgatgt cgcgccagaa gtcaaagtcc tcaccgggca gaatgaccat
     1081 cccgtccagg tcacgggcgg ggatcgagat gttgttgccc cagccgttgc ccgccgccgg
     1141 gacgtagtac gtggtccacg tcgacaggcg gatcagcttg ggcagaatct cctgcgccgc
     1201 agcggtggtg agggtgggct ccacgaacga cgcgctgagg gccaccccgg ggacctcgat
     1261 cccggctccc cgttgctcga ggccggccac gatcgcgttg accgtcaccg gcacgttcag
     1321 gtcacggccg cggatcgcgg gcttcaccgc agtcgggccg gccgcgccga acgtgaaccc
     1381 cgcatcggtc ggggcacgcc ggaccgcggc gccgagggtc gccgctgccg cgctcagcgc
     1441 cgtacggtcg aggatgaccc cgtaacgttc gtccgagcgg gtaccgaacg agatcgcggc
     1501 ctggatatcc gcaggcgcga ccgggtacgc gtcgtcgccg tcggtcaggg ccagtggtgc
     1561 ggcgaacatc gcccgcgcgg ccgcggccgc ttccgccgcc tcgcgctggg tgaccacgaa
     1621 cggaatcggc tcggagtcga aggtcaccgc cggattggac acggccggct ggagcagggc
     1681 cgcggagagg gccgcccgga tctggtcggg atggattcgg tggcccgcgg cggggggctg
     1741 gatcgaatac gtgccgtcag ggttggtcac gaccgttgcg tcgaccggcg cgacgaaggc
     1801 gccggtcgcg gtctcgatca cgagccggtc cagctggccc gagtcaaggg ccaccgcgtc
     1861 atcgacattc gcgcccacga ccagggtcgc gagccgcgtc tggccgtcgt tccaggcatc
     1921 gccccggcga ccgaccgcca tggccgccgc gacgaggcca tcaaaatcgt gcgcccgatc
     1981 gacgcgactc agcggtacgg tggtgctgac gccgtccacg gtgataacca gctcgccggc
     2041 ggtgaggtca ggcagctccg atcgaaggcg ctcgccggct tccgccgcgg tcagcaggcc
     2101 cacgtcgacg ccggccaccg acacgcgcgg catgacgcgc ccgtcgaaaa cgacgcccat
     2161 cccggccgcg gccagcgtcg gaatggcgag catggcggcg aacacgagcg cggccacggc
     2221 agcgccaacc gccgtccgcg ggagcggacg gacacggggg aggagttccg cgtcgggcgt
     2281 gacttcggcg ccgggggtcg caaccgcggg ggttcgcatc gatgtgccgc ggattgtcgc
     2341 cgaactgcct gtcgcgtgtc gacgcgacct cggtgctata ctccgccgcc gatcggcccc
     2401 caggccgaga gacccccatt tcactcacga ggactgcctg ttcgtggcac gcaaagcccg
     2461 cgcctggaag ggtgcccgca ctccatccgc gctcaaacgc gcccgccagt cgctccgtcg
     2521 gcgcgcggtg aacaagcgca cccggagcga ggccaagacc ctcgtccagc aggccgccag
     2581 cgtggcgacc ggccgctccg agggcgatga ggccgtggct gtcagcgccg ccatcagcgc
     2641 gctggacaag gccgccgaga aggggatcat ccatccgaac aacgccgccc ggcgcaagag
     2701 ccggttgatg gccaaggtca acgccgtcca cgctcaggat gcccacgcgg gggcgggagc
     2761 caagcgccgg accacggccg gcaaggccga ccagcgcaag aaggtcgccg cggccaaggc
     2821 ctccaaggcc gccgccgcac gctccgccga ccggccgcgc acggctgccg gcaaggcccg
     2881 cgtcgctgtg acccgcgcca cccgagagac gaccgccgcg cagcgacggt ccgccaagca
     2941 ggacgccccc gagaccgagc ccgagtccta gcccgctcgc ctgaacggcg cgcggccggc
     3001 gtacacggcc cgctccccaa gctcctcttc gatccgcagc aggcggttgt acttcgccgt
     3061 ccgctcggat cggctcatgc tgccggtctt gatctggccc atgcccatcg cgaccgcgag
     3121 gtcggcgatg gtcgtgtcct cggtctcgcc cgaccgatgg ctgaccacgg cgccccagcc
     3181 ggcggcctcc gcggtccgca ctgcgtcgat cgcctcggtc agggtgccga cctggttgac
     3241 cttgaccagg atcgcgttcg cggcctgctc ggcgacggcc cgctcgagtc gtgcctggtt
     3301 ggtcaccagc agatcgtcgc ccaccagctg cacggtcgca cccaaccgct cggtgagggc
     3361 cgtccagccg tcccagtcgt cctccgccag gccatcctcc aggctgacga tcgcgtaccg
     3421 atcggtccag tccgcccaca gatcgatcat gccgctggcg tcgagcgtcc gaccctcgct
     3481 ggcgagggcg taggtcccgt ttccgaacag ctcgctggcg gccgggtcga gggcgatcgc
     3541 gacctgctca ccggcccggt atccggcgcg gccgatggcc tccgtgacga gctcgatggc
     3601 cgcctcgttg ctgggcagcg ccggcccata gccaccttcg tcgccaaccg cggtcgggag
     3661 gccgcgcgcg cgaagcagcg ccgcgagcgc gtggaagatc tcggccgtcc agcggatgcc
     3721 ctggctgaag gtggccgccc ccagcgggac gaccatgaac tcctggaagt cggtcccacc
     3781 ggcggcgtga cgtccaccgt tgaggatgtt caccatcggt accggcaagg tcgccgcccg
     3841 ctccccgccc agataccgat acaacggctg gccggcggcg gccgcggcgg cgtgggcggt
     3901 ggccagcgag actccgagca ccgcatttgc gccgagccgc gccttgttcg gcgtcccgtc
     3961 gagatcgatc agcgcctggt cgaggcccgg ctggtcggtg gcatcgcgtc cggtgacggc
     4021 gcccgcgatc tcgccccgga cattcgccac ggcccggagc acgcccttgc cgccgaatcg
     4081 cgaggcatcg ccgtcgcgga gctcgacggc ctcgtgggtc ccg
//
LOCUS       DASYGU010000039         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_57492, whole genome shotgun sequence.
ACCESSION   DASYGU010000039 DASYGU010000000
VERSION     DASYGU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57492"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGO32_00990"
     CDS             complement(<1..758)
                     /locus_tag="VGO32_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7199358.1"
                     /translation="MEIAPNVHAVPMKSAGAFLITEDRLTLIDAGLAGSKPMLVRYLA
                     DIGRSIDELDRIVCTHGHPDHVGGVREIADGEPIEVFIHPADLEGVSHTVREALAPTA
                     GGSLRGRLLQAVTPHPGTATSLNDGEVLPALGGLEAHTISGPMEIAPNVHAVPMKSAG
                     AFLVTEDRLTLIDAGLAGSKPMLVRYLADIGRSIDELDRIVCTHGHPDHVGGVREIAD
                     GEPIEVFIHPADLEGVSHTVREALAPTAGGSLRGR"
     gene            complement(792..>1726)
                     /gene="rsgA"
                     /locus_tag="VGO32_00995"
     CDS             complement(792..>1726)
                     /gene="rsgA"
                     /locus_tag="VGO32_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV7199359.1"
                     /translation="DGVAPLSVPTLTGTVLRARSGFFTVSTESGTYECQLRGRLKRER
                     QSEDLVVIGDEVTLTPLTDTDGVIEEVAPRRSRFSRRQPGNRGSWKEDLMVANLDQLV
                     AVFACADPAPHMRMIDRFLVIAEYNEVPTLIVANKIDLSSTAEAQALFAAYERIGYPV
                     VYASAKTGQGVDELRARLAGRISLVAGPSGVGKSTLLNVVHPGLNLATGPISEALHKG
                     RHTTTSSELLPVAGPMGGHVADSPGLRQVGLWEIPVTEIAWCYPDLRPFLGACRFADC
                     SHGPEPGCAITAAVAAGQISAARHDSYRRLVESV"
BASE COUNT          262 a          571 c          617 g          276 t
ORIGIN      
        1 cggccgcgca ggctgccacc tgcggtcggg gccagcgcct cgcgaacggt atggctgacg
       61 ccctcgagat cggcggggtg gatgaagacc tctatgggct ccccgtcggc aatctcgcgg
      121 acgccgccga cgtggtccgg atgaccgtgg gtgcacacga tccgatccag ctcgtcgatg
      181 gaccggccga tatcggccag atagcgaacg agcatgggct tcgatccagc cagccctgcg
      241 tcgatcaggg tcagtcggtc ctcggtgacg aggaacgcgc ccgcgctctt catcgggacg
      301 gcgtggacgt tcggggcaat ctccatcggc ccgctgattg tatgggcctc caggccgccc
      361 agcgcgggca ggacctcccc gtcgttcagg gacgtggccg tcccggggtg cggggtgacg
      421 gcctggagca ggcggccgcg caggctgcca cctgcggtcg gggccagcgc ctcgcgaacg
      481 gtatggctga cgccctcgag atcggcgggg tggatgaaga cctcgatggg ctccccgtcg
      541 gcaatctcgc ggacgccgcc gacgtggtcc ggatgaccgt gggtgcacac gatccgatcc
      601 agctcgtcga tcgaccggcc gatatcggcc agatagcgaa cgagcatggg cttcgatccg
      661 gccagccctg cgtcgatcag ggtcagccgg tcctcggtga tgaggaacgc gcccgcgctc
      721 ttcatcggga cggcgtggac gttcggggca atctccatcg gcccgctgat tgtatgggcc
      781 tccaggccga attagaccga ttccaccagg cgtcggtagg aatcgtgccg ggcggcgctg
      841 atctgcccgg cggccaccgc cgcggtgatc gcacagcccg gctccgggcc atggctgcaa
      901 tccgcgaacc ggcaggcgcc caggaacggt cggaggtccg ggtagcacca cgcgatctcg
      961 gtgaccggga tctcccacag gccgacctgg cgcaggcccg gcgagtcggc gacgtgcccg
     1021 cccatcggtc ctgccacggg gaggagctcg gacgaggtcg tcgtgtgccg tcccttgtgc
     1081 agcgcctcgc tgatcggccc ggttgccagg ttcaggccgg ggtgcacgac gttgagcagg
     1141 gtgctcttgc ccacgcccga tggtccggcg accaggctta tccggcccgc gaggcgcgcc
     1201 cgcagctcgt caacgccctg gccggtcttc gcgctggcgt agacgaccgg gtagccgatt
     1261 cgctcgtacg cggcgaacag tgcctgtgcc tccgcggtgg agctgaggtc gatcttgttg
     1321 gcgacgatga gcgtcggaac ctcgttgtac tcagcgatca cgaggaagcg gtcaatcatc
     1381 cgcatgtggg gagccgggtc cgcgcaggcg aagaccgcca ccagctggtc gagattggcg
     1441 accatcaggt cctctttcca cgacccgcgg ttgccgggct ggcggcgcga gaagcgactc
     1501 cgccggggag cgacctcctc gatgacgccg tcggtatcgg tcagcggggt gagggtgacc
     1561 tcgtcgccta tgacgaccag gtcctccgac tggcgttcgc gctttaggcg gcctcggagc
     1621 tggcactcgt acgtgccgga ttcagttgaa acagtgaaga acccgctgcg cgcgcgcagc
     1681 acggtcccgg tcagggtggg aacagacaag ggtgcgactc catcag
//
LOCUS       DASYGU010000040         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_468921, whole genome shotgun sequence.
ACCESSION   DASYGU010000040 DASYGU010000000
VERSION     DASYGU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468921"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGO32_01000"
     CDS             <1..558
                     /locus_tag="VGO32_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV7199360.1"
                     /translation="DGVELDVDVVSDGTAWCVPGILEQRDPPGIHSGDSLAVLPPQRI
                     SRSAQERAAEAAGRIALALGVRGILNVQAIVQGERVVVIEANPRASRTVPIVAKATGR
                     DVVAAAVRCALGSSLAGAGLTPGLAPDGPMVAVKAPIDSLWRLPGVTAELGPEMRSTG
                     EVLGLGEDYAGALLSAEEAVAAHRP"
     gene            complement(577..1239)
                     /locus_tag="VGO32_01005"
     CDS             complement(577..1239)
                     /locus_tag="VGO32_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199361.1"
                     /translation="MPATQSRSAQDRAKARRRARQVAKGDQPLPEEETAAAASTANEP
                     RPPNFLARLFPPAPPLPGKGDPMAGFQYTGRFRRLVAGAWLLAHHPLTWGVAGAGWAI
                     LQLALLVTARQGMIAFGITLGQYGLLIGAGWFGWWRPWLFGTAAGVFGVLLHAVIAGM
                     VTLQSGETITAALAVTGSLAALVLLYGLVGAFAGFYGGYLRRRMAIPTGKNAKGPKPK
                     RR"
     gene            1284..>1998
                     /locus_tag="VGO32_01010"
     CDS             1284..>1998
                     /locus_tag="VGO32_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEV7199362.1"
                     /translation="MPQDPGQRHPGGVGLVLLRPRSLMHLDPARAHRPISGGLAFAVL
                     LLGALVTAGVRAAPLEGDPAPLASVDHVVISEVMTGGTSASDEFVELYNPGSTARSLD
                     GLELVYVTATGATVTRKAVWDPGAQIAGRSHLLVANEAGIFAGVADLTYANGLAATGG
                     SMALRGVAASTAVDAVGWGNATGTWHEGTVASAPSAGSSLERLPGGSQGSGQDSDQNA
                     VDFIVRGAPDPRNAASLPVP"
BASE COUNT          264 a          695 c          765 g          274 t
ORIGIN      
        1 gacggggtgg agctggacgt ggacgtcgtc tcggacggga cggcatggtg cgtgcccggc
       61 atcctcgagc agcgcgaccc gccgggcatc cattcgggcg actcgctggc cgtccttccc
      121 ccgcagcgca tctcgcgttc cgcccaggag cgtgccgccg aagcggccgg gcggatcgcg
      181 ctggcgctcg gggtgcgcgg catcctcaac gtgcaggcca ttgtccaggg cgagcgggtg
      241 gtcgtgatcg aggccaatcc acgggcgtcg cggacggtcc cgatcgtggc caaggccacc
      301 ggccgggacg tcgtggcggc cgccgtccga tgcgcgctcg gatcgtcgct ggcgggggcc
      361 gggctgacgc cgggcctggc tcccgatgga ccgatggtcg cggtcaaggc accgatcgac
      421 tcgctctggc gcctgcccgg ggtcaccgcc gagctcggtc ccgagatgcg ctcgaccggc
      481 gaggtgctgg gtctgggcga ggactacgcg ggtgcgctgc tgtcggcgga ggaggccgtc
      541 gccgcccatc ggccataggg aggccgcgcg acccggctag cggcgcttgg gcttgggccc
      601 ctttgcgttc ttgccggtcg ggatcgccat ccgccgccgg aggtagcccc cgtagaagcc
      661 ggcaaaggcg ccgaccagcc catagagcag gacgagggcc gcgaggctac cggtaacagc
      721 cagcgcggcg gtgatggtct cgcctgactg gagcgtgacc atacctgcga tcacggcgtg
      781 caggaggact ccgaacacgc cggccgcggt gccgaacagc cagggccgcc accagccgaa
      841 ccaaccggcg ccgatcagga ggccgtactg gcccagggtg atgccgaagg cgatcatgcc
      901 ctgccgggcg gtcaccagca gcgcgagctg gaggatggcc cacccggccc ccgcgacgcc
      961 ccaggtcaag ggatggtggg cgagcagcca cgccccggcg acgaggcgac gaaaccggcc
     1021 ggtgtactgg aagcccgcca ttggatcgcc cttgcccggc agcggcgggg ctggaggaaa
     1081 gagcctggcc aggaagttcg gggggcgcgg ctcgttggcg gtcgaagccg cggccgcggt
     1141 ttcctcctcg ggcagtggct gatcgccctt cgccacctgt cgggcgcggc gacgcgcttt
     1201 ggcgcggtcc tgtgcggagc gggattgcgt ggcgggcatc ggccgcggat tgtacgcgtg
     1261 agctacgcgc acggtaaggg ggcatgcccc aggatcccgg ccagcgccac ccgggcggcg
     1321 tcggactcgt tctcctccga ccgaggtcgc tcatgcacct ggaccctgcc cgcgctcatc
     1381 gtcccatctc gggagggctc gccttcgccg tgctcctcct cggcgccctg gtcacggccg
     1441 gggtccgcgc ggcgcccctg gagggggatc ccgccccgct cgcctcggtg gaccacgtcg
     1501 tcatctccga ggtcatgacc ggcgggacga gtgcgtcgga cgagttcgtg gagctgtaca
     1561 accccggctc gaccgcccgg tcgctggacg ggttggagct cgtctacgtg accgcgaccg
     1621 gggcgacggt cacgcggaag gcggtgtggg atcccggcgc ccagatcgcg ggccggtccc
     1681 atctgctggt cgccaacgag gccggaatct tcgccggcgt cgcggacctg acctacgcca
     1741 acgggctcgc ggcgaccggc ggcagcatgg ccctgcgcgg cgtcgcggcc tcgacggcgg
     1801 tggacgcggt tggctggggc aacgcgaccg gcacctggca cgaaggcacg gttgcgtcgg
     1861 ccccttcggc cggctcgagc ctggagcggc tgccgggggg aagccagggt tcgggccagg
     1921 acagcgatca gaacgccgtc gacttcatcg tgcgtggggc gcccgatccg cggaacgcgg
     1981 catcgttacc ggtcccga
//
LOCUS       DASYGU010000041        10757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_983317, whole genome shotgun sequence.
ACCESSION   DASYGU010000041 DASYGU010000000
VERSION     DASYGU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10757
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983317"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGO32_01015"
     CDS             complement(<1..434)
                     /locus_tag="VGO32_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199363.1"
                     /translation="MASQQRSGPAITVKDLTISFSGPAGPLTALGGVSGEVAGGELLS
                     VIGPNGSGKSTLLRALAGLLLPTAGRIGLPPDGSPPRVGDGRVGLVFQQPRLLGWRTT
                     LDNVTLPLELEGTAATERRAAGERALAQVGLTDVAGLRPRELS"
     gene            complement(776..1459)
                     /locus_tag="VGO32_01020"
     CDS             complement(776..1459)
                     /locus_tag="VGO32_01020"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV7199364.1"
                     /translation="MLEFIDQTVIPFFLDLYDALGYIGVAVGVALETFIPIIPSEVIV
                     PMAGWKIAQSGADPTVTEWLTDAPWTFIGVMVAATIGAAVGSLFGYLIGAWGGRPLLD
                     RYGRYVHIQADDLDRADAWFARYGGWAVFFARFVPLLRALISYPAGVARMPLGRYMVF
                     SVAGSIPWNLALVTAGWVLGENYEALYEAIRPFEILTYVVVVLVGAFVLIRWARGRGT
                     PREIDRPTG"
     gene            complement(1469..2323)
                     /locus_tag="VGO32_01025"
     CDS             complement(1469..2323)
                     /locus_tag="VGO32_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7199365.1"
                     /translation="MRRFPDPTSRLVSSRGAQYRLGGAVPSPAAAFLVALILLPTLFW
                     LVALKRTTTYLQPEEWLMQNIVVPFQAWMEANLSPVDAVLTAISALGSGWFVALGFAV
                     CGLVALAKGRRDLALLLALGTLAFPMEWALKFFVAMPPISIGELIASMFNVGGFGLDD
                     IAVFPAGHALRSMVFYGLVAFCIARLSSDRRQGMIAYAVAAVLIASIGLVRIYLNLHY
                     PLDVLGGWIAGASLVSILVAIHVLNVDEQMRVTERQRVATAGTTAETAEAAASASAAA
                     SAAPTVEA"
     gene            2344..3861
                     /gene="hutH"
                     /locus_tag="VGO32_01030"
     CDS             2344..3861
                     /gene="hutH"
                     /locus_tag="VGO32_01030"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEV7199366.1"
                     /translation="MAPQPPIQLTGRDLTVADVVSVARGGRSVAVADEARQHMAVSRA
                     VIERVVASGETVYGVTTGFGDLADVRISPEQTATLQRNLVRSHAAGFGDPLPTEVVRA
                     MLLLRAAALAVGLSGVRPELVDLLVAMLNAGVHPVIPSRGSVGASGDLAPLAHLAQVV
                     IGEGEAEVGGAALHGADALAAAGLEPMELQAKEGLALLNGTQLMAAIGALALTDAGIL
                     VRTADVTGAMSLEAMLGTAAAFDESLMWARPHPGQLASAARLRGLLADSEIGDSHRSS
                     PHKVQDPYSLRCMPQVHGAVADALSPLDDAVSVELNAATDNPLVFPDGRVVSGGNFHG
                     EPLALALDHARLALTALATISERRTARILDARLSGLPAFLAADPGIESGLMLAQYSAA
                     AAVNELKTTAHPAAADTIPTSANQEDHVSMGATSALLLRDAVDRATTVLAVEALCAAR
                     GLDLRAPLRPGRGVAAAHAAVRRVVPPLDGDRSPAPDITALSEVIRAGSLAAVAG"
     gene            3865..4590
                     /locus_tag="VGO32_01035"
     CDS             3865..4590
                     /locus_tag="VGO32_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199367.1"
                     /translation="MDGLTFSLVDEAAIDAIPPPARQGARCQTCDYWERLEGSREGDD
                     TTTRRSAKKAALMAGARLAGSYGMVATDASGEILGYAQFGPISAYPRAQLIRDRYAEL
                     PESPAPWVMTCLQVVPDAPKRVGIGQALAEAVCTELDRRGVIAVETYLERASDPWLPS
                     GGPAQAYEAAGFVRAAGDEQFPVYRRELAGEAEVGWDDLLAKARPPVEGDAWPIPLPK
                     GPKEEDVFRLPPKPDRPNPFGDD"
     gene            4594..5322
                     /locus_tag="VGO32_01040"
     CDS             4594..5322
                     /locus_tag="VGO32_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEV7199368.1"
                     /translation="MIEAFVWAFIAASALLLGAVAGLAMKINRTVIGLALAFGAGALI
                     SAVAYELTAEAFEQGGGPLLAAGLAAGALTFFTGNRWLHTHGTKRRRLTVKEQGSQGQ
                     GLSIVLGAMLDGIPESVVLGASLIGGGAVSPAFLVAVLLSNVPEGFSASADLHQEGRS
                     SRWILGLWFAVVLVSATSAALAFLLLDEAGPAVPFVQALAAGGLLTMLVDTMIPEAFE
                     DGGDLAGLVTVFGFTLAFLLSALT"
     gene            5346..6548
                     /locus_tag="VGO32_01045"
     CDS             5346..6548
                     /locus_tag="VGO32_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199369.1"
                     /translation="MRGRISILAVTLLVAGCQSQTPSGSASPSSTASETQSAAPSVAA
                     SATDPGPAAWSETGSFNDTGGFSLVIDIAQNGTVVVAVGTQYVAPIPNFGFPPDHDGR
                     LWISEDGYTWSDAAPAGVFDGVSLNQVLATPAGGFVAFGFGPAQRAWGSADGETWTEI
                     PSPFPANSTVTSLASGAQGYLTLVDGPARQLWFSGDGAAWAATYALADQAVSIGAGDE
                     GFAVAGTANPGTAQAAPFGIASSDGSGWVPAATPPSVASGAVHVAPHAGDWYAMANGD
                     LTATTPVSFSANGLDWAQSGSMPLAEVQADATTTCREFVTALHGSTPWLVATSRLSFP
                     CSEGGFVVHGTQRASTDGTTWTDLPFAAGTPGQTGSGSAVFATVMVNGRLVLAGQSNS
                     AATFWIGE"
     gene            6604..8289
                     /gene="hutU"
                     /locus_tag="VGO32_01050"
     CDS             6604..8289
                     /gene="hutU"
                     /locus_tag="VGO32_01050"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEV7199370.1"
                     /translation="MTTRAEPRSGPRTVRAPRGTELSTKGWQQEAALRMLMNNLDPDV
                     AEDPDHLIVYGGTGRAARSWEAFDAIVRELRRLENDETLLIQSGKPVGVFRTTPDAPR
                     VLIANALLVPHWATWENFRELERAGLTMYGQMTAGSWIYIATQGIIQGTYETFAECGR
                     QHFGGSLRGTATLTAGLGGMGGAQPLAVTMNEGAVLAIEVDPARAERRHVAGYVDEVT
                     TDLDDALAKVDGWRARGEARSIALVANAADVEPELVRRGWRPDVVTDQTSAHDPLGGY
                     VPGGIGLPDALALREASPDEYVARAKASIADHVRAMLAFQQAGSVVFDYGNNIRAFAR
                     EAGVADAFAIPGFVPAFIRPLFCEGKGPFRWAALSGDPNDILKTDAALAELFPDDASL
                     QRWLRLAEGKVPFQGLPARICWLGYGERARAGLRINDMVRSGELAAPIVIGRDHLDAG
                     SVASPYRETEGMRDGSDAIADWPVLNALLNTAAGATWVSVHHGGGVGIGYSLHAGMVI
                     VADGTDAAAARLERVLTTDPGMGVIRHADAGYERALEVARERGVRVPMAEGEA"
     gene            8366..8824
                     /locus_tag="VGO32_01055"
     CDS             8366..8824
                     /locus_tag="VGO32_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEV7199371.1"
                     /translation="MRHRSPIALVSVALFVAACASPGTGGMTSVFDLANGQCFNDAGE
                     TIVDEVEVVDCANAHDYEIYAVDDYPAESGADYVGEEEIGTFVQTTCLDGFEEFVGMT
                     YEESELDIYYLAPTEDSWGSGDREVLCAAYLPSDTGDPEPVEGTLEGSNR"
     gene            complement(8821..9921)
                     /locus_tag="VGO32_01060"
     CDS             complement(8821..9921)
                     /locus_tag="VGO32_01060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV7199372.1"
                     /translation="MSEPARPALAGFQPETVDDPTLINLHDYEQAAVARLPAGALAYY
                     RGGANDERTLTENRAAFDRRWIVPRVLRDVSTVDTMVEVLGRRWPSPLFLCPTAFHLM
                     AHPDGELGTARAAAARGITMTLSTSSSTDLAAVAEIGGPRWFQVYLLADPGARRALVE
                     RAIASGYEAMVLTVDLPRIGRRERDIRDRFAIPDDIHVPNPAVAAGVPTEHGLSVPFV
                     ERITWDDVAWLTGFGRPVVIKGILHPDDARMAVEHGVAAIQVSNHGGRQLDGAIASLE
                     ALPAVLQAVGGRVPVLLDGGVRRGSDVVIALALGATAVGIGRPTLWGLAVDGEAGVGR
                     VLDLLTAEIELAMALAGAPSPASLTPDLVVSP"
     gene            10020..>10757
                     /locus_tag="VGO32_01065"
     CDS             10020..>10757
                     /locus_tag="VGO32_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199373.1"
                     /translation="MSFDPSAPLPGAPDPWAGSEMPALRDGPPWAMTEMIASEPALAG
                     RLVPRLTADPALGRVADAVREAAARGETITVTGCGTSHHAALAIASLIRGGLADNGQR
                     TDGVRAAQAFELTGSPPTPGVVIAVSHEGGTEATNRSLAHAGGRDAPTALITVSDRSP
                     GAALARDVLTTGEQDQSWCHTVGYLSPLVVGACLRAAVRGETLSAHAVADQVAAGSDS
                     SAAEGAAAALAGADRLLIVGSGADWAAA"
BASE COUNT         1583 a         3761 c         3844 g         1569 t
ORIGIN      
        1 gacagctcgc ggggccgaag gccggcgaca tcggtcagtc caacctgggc cagtgcacgc
       61 tcccctgccg ctcgccgttc cgttgctgca gtgccctcca gctcgagcgg gagggtcacg
      121 ttgtccaacg tcgttcgcca ccccaggagg cgtggttgct ggaagacgag accaacccgg
      181 ccatcaccca cgcgcggcgg cgatccatcg ggcggcagac cgatgcgtcc cgcggtcggc
      241 agcagcagtc cggccagcgc cctcaggagg gtgctcttgc cggatccatt gggaccgatg
      301 accgacagca gctccccgcc agccacctcc ccgctcaccc cgcccagggc ggtgagcggc
      361 ccggcagggc cggagaaaga gatcgtcagg tccttgaccg tgatcgcggg gccggatcgt
      421 tgttggcttg ccattggtct cccatgcgcc agcgcagggg ggcgtgggag cggcgaggcc
      481 atcggtccag cgcgccgcgg atcccgggcg gcgcgcagcg ccgatcggtc tcgcgggtcg
      541 tcggaggaac gcctcgtgct cgccctctcc catccggact gtgaccgtcg gccctgtctg
      601 cccggctgat gccggggtca ggtcggcccc gcgaggtggt tccggcggcc ttctccgtgc
      661 cgccgacctc gaggggttcg cgggctggcc cgactcagtc agaccgaccg ccggtgggga
      721 gtttcacccc gccccgagag cgatgaattc gttcagttgc cctttgatcg tgccactacc
      781 cggtaggccg gtcaatctca cgcggtgtgc cacggcctcg ggcccatcgg atgaggacga
      841 aggcaccgac caggacgacc acgacgtagg tcaagatctc gaacgggcgg atggcctcgt
      901 acagggcctc gtagttctcg cccagcaccc agccggcggt gacgagggcc aaattccacg
      961 ggatcgagcc ggccaccgag aagaccatgt agcggccgag cggcattcgg gccacgcctg
     1021 ccgggtagct gatcagggcg cggagcagcg ggacgaaccg ggcgaagaac accgcccagc
     1081 cgccgtagcg ggcgaaccag gcgtccgccc ggtcgagatc atcggcctgg atgtggacgt
     1141 agcggccgta tcggtccagc agaggtcgcc caccccaggc gccgatcaga tacccgaaca
     1201 gcgagccgac cgccgcgccg attgtcgccg ccaccatgac cccgatgaag gtccagggcg
     1261 catcggttag ccattcggtt accgtggggt cggcccctga ctgggcgatc ttccagccgg
     1321 ccatggggac gatgacctcg gacgggatga tcggaatgaa tgtctcgagc gccacgccga
     1381 cagcgacacc gatgtagccg agggcgtcgt acaggtccag gaagaacgga atgaccgtct
     1441 ggtcgatgaa ctccagcacg tccggccgtc aggcctcgac ggtgggtgct gccgaggcgg
     1501 cagcgctcgc cgatgccgcc gcctccgccg tttcggcggt ggtcccagct gtcgccacgc
     1561 gctgccgttc ggtgacgcgc atctgctcgt ccacgttcag gacgtggatg gccaccagga
     1621 tcgagaccag cgatgcgccc gcgatccagc cgccgagcac gtcgagcggg tagtgcaggt
     1681 tcaggtagat ccggaccagg ccgatgctcg cgatgaggac cgcggcgacc gcgtacgcga
     1741 tcatcccctg ccgccggtcg gacgagaggc gcgcgatgca gaacgcgacg aggccgtaga
     1801 agaccatgga ccggagggca tggccggcgg ggaagacggc gatgtcatcg agcccgaagc
     1861 caccgacgtt gaacatggac gcgatgagct caccgatcga aatgggtggc atggcgacga
     1921 agaacttcag cgcccactcc atcgggaaag cgagcgtgcc gagcgcgagc agcagagcca
     1981 ggtcgcggcg gcccttggcc aacgcgacca gcccgcagac cgcgaagccg agggccacga
     2041 accagcccga ccccagggcg ctgatcgcgg tcagcaccgc gtccaccggg gacaggtttg
     2101 cctccatcca cgcctggaat gggacgacga tgttctgcat cagccattcc tcgggctgga
     2161 ggtacgtcgt cgtccgcttg agggcgacga gccagaacag ggtcgggagc aggatcaggg
     2221 cgaccaggaa tgccgcggct gggctcggga cggcgccgcc gagccggtac tgcgcacctc
     2281 ggctcgacac caggcggctg gtcgggtccg ggaacctccg cacgtccgta gtatagaaac
     2341 gaggtggctc cccaaccccc catccaactg accggtcgcg acctgaccgt cgccgatgtc
     2401 gtatcggtgg cgcggggcgg tcggtcggtc gcggtcgcgg acgaggcccg ccagcacatg
     2461 gcggtgtccc gcgccgtgat cgaacgggtc gtggcgtcgg gcgagacggt gtacggggtc
     2521 acgaccggct ttggcgacct cgccgacgtg cggatctcgc ccgagcagac ggccaccctc
     2581 cagcgaaacc tggtgcggag ccacgcggcc ggcttcggtg atccgctccc gaccgaggtc
     2641 gtgcgcgcca tgctcctgct ccgggccgcc gccctggcag tgggcctatc cggcgtccgg
     2701 ccagagctcg tggacctcct cgtcgcgatg ctcaatgcgg gcgtccaccc cgtgattccg
     2761 tctcggggca gcgtcggcgc ctccggtgac ctcgctccgc tcgctcacct ggcccaggtg
     2821 gtgatcggcg agggtgaggc ggaggtggga ggcgcggccc tgcacggcgc cgatgccctc
     2881 gcggcagccg gcctggagcc gatggagctg caggcgaagg aaggtctcgc cctcctgaac
     2941 gggacccagc tgatggccgc catcggcgcg ctggcactga ccgatgcggg aatcctcgtc
     3001 cggaccgcgg acgtgaccgg cgccatgagc ctggaagcga tgctcggaac ggctgccgca
     3061 ttcgacgagt cgctcatgtg ggcgcggccg cacccgggcc agctcgcgtc cgccgcgcgg
     3121 ttacggggcc ttctcgcgga cagcgagatt ggggacagtc atcggtcgag cccgcataag
     3181 gtccaggacc cgtactcgtt gcgctgcatg ccgcaggtcc acggcgccgt ggccgacgcc
     3241 ctatcgccgc tcgacgacgc ggtctcagtc gagctaaacg cggccaccga caaccccctg
     3301 gtctttcccg acggccgggt ggtgagcggc ggcaacttcc acggcgagcc gctggccctg
     3361 gccctggacc atgcccggct cgcgctgact gcgctggcca cgatctccga gcggcgaact
     3421 gcgcggatcc tggacgcccg cctttcgggc ttgcccgcct tcctggccgc ggacccgggg
     3481 atcgagagcg ggctgatgct ggcccagtac tccgctgcgg ccgcggtcaa cgagctgaag
     3541 acgaccgccc atccggccgc ggccgacacg atcccgacca gcgccaacca ggaggaccac
     3601 gtcagcatgg gcgccacttc tgccctgctc ctccgggacg cggtggaccg ggcgaccacc
     3661 gtcctggcgg tcgaggcgct gtgcgccgcc aggggtctcg acctgcgcgc cccgttgcgg
     3721 ccaggacgag gtgtggccgc cgctcatgcc gcggtgcgcc gcgtcgtccc gccgctggac
     3781 ggagaccgat cgccggcgcc cgatatcacg gccctgtcgg aggtgatccg agccggctcg
     3841 ctggcagcgg ttgccggctg accgatggac gggctcacct tctcgctggt cgacgaggca
     3901 gccatcgatg cgatcccacc gcccgcgcgg cagggagcgc gctgccagac gtgcgactac
     3961 tgggagcggc tggaaggcag ccgggagggg gatgacacga ccacgcggcg gtccgcgaag
     4021 aaggcggcgc tcatggccgg ggcgcggctg gccggctcgt acgggatggt cgccacggac
     4081 gcgagcggcg agattctggg gtatgcccag ttcgggccga tcagcgccta tccgcgtgcc
     4141 cagctcatcc gggatcgata cgcggagctt cccgagtcgc ccgccccgtg ggtcatgacc
     4201 tgcctgcagg tcgtccccga cgcgcccaag cgtgttggga tcggtcaggc gctggcggag
     4261 gcggtgtgca ccgagctcga ccgccgcggg gtgatcgcgg tcgagaccta cctcgagcgc
     4321 gcctccgatc cgtggctgcc gtccggcgga cccgcccagg catacgaggc cgccggcttc
     4381 gtccgggcgg ccggggacga gcagttcccg gtctaccgcc gcgagctggc cggcgaagcc
     4441 gaggtgggct gggatgatct gttggccaag gcgcgtccgc ccgttgaggg cgatgcgtgg
     4501 ccgattccgc tgccaaaggg gccgaaggag gaggacgtgt ttcgcctccc gcccaagccg
     4561 gatcgaccga acccgttcgg cgatgactga ccgatgatcg aggcattcgt ctgggcgttc
     4621 atcgcggcgt cggcactctt gctgggcgcg gtggctgggc tcgcgatgaa gatcaaccgg
     4681 acggtgatcg gattggccct cgcgttcggc gccggggccc tgatcagcgc ggtggcgtac
     4741 gaactcaccg cagaggcgtt cgaacagggc ggcggcccgc tcctggccgc cgggctggca
     4801 gccggcgcgc tgacgttctt caccggcaat cgatggctcc acacgcatgg caccaagcgg
     4861 aggcgcctca ccgtgaagga gcaggggagc cagggtcaag gactgtccat cgttctcggc
     4921 gcgatgctgg acggcatccc cgagtcggtc gtcctgggcg cttccctgat cggcggcgga
     4981 gccgtctctc cggcattcct ggtcgccgtc ctgctgtcga acgtgccgga ggggttctcc
     5041 gcctccgccg acctccacca ggaagggcgt tcgtcacgct ggatccttgg cctgtggttc
     5101 gcggtggtgc tggtgtctgc gacttcggcc gcgcttgcgt tcctcctcct cgacgaggcc
     5161 ggacccgccg tcccgttcgt ccaggctctt gccgccggcg gcctgctcac catgctggtg
     5221 gacaccatga tcccggaggc attcgaggac ggcggcgacc tcgctggcct ggtcaccgtg
     5281 ttcggattca cgctcgcctt cctgctgtca gccctgacct gaccgggcgt atgctggccg
     5341 cggcgatgcg cggtcggatt tcaatcctcg cggtaactct cctcgtcgcc ggctgccaat
     5401 cacagacccc gtccgggtcg gcgtcgccct cgagcaccgc cagcgaaacc cagagcgccg
     5461 caccctcggt cgcagccagc gcgaccgatc cggggcctgc ggcatggtcg gagacgggtt
     5521 cgttcaatga caccggcggc ttctccctgg tcatcgacat cgcccagaac ggcaccgtgg
     5581 tcgtggcggt cggcacgcag tacgtggctc cgatcccgaa cttcggcttc ccgccggacc
     5641 acgacggacg gctctggatc tcggaggatg ggtacacctg gtcagacgcc gcgccggccg
     5701 gggtcttcga cggcgtctcc ctgaatcagg tgctcgccac gccggccggt ggcttcgtgg
     5761 cgtttggatt cggtccggct cagcgcgcct gggggtccgc ggacggcgag acctggacgg
     5821 agatcccgag tccattcccg gccaactcaa cggtcacgag tctcgcgtcg ggtgcccagg
     5881 gatacctgac cctcgtcgat ggcccggcgc gccagctgtg gttctccggt gacggtgccg
     5941 catgggcagc cacctatgcg ctggccgacc aggccgtctc catcggtgcc ggcgacgagg
     6001 gcttcgccgt ggccggaacg gcgaacccgg gcactgcgca ggccgcgccg ttcgggatcg
     6061 cgtcgtccga tggatccggc tgggtgccgg cggcgacgcc accgtcagtg gcttccgggg
     6121 ccgtgcacgt ggccccgcac gcgggcgact ggtatgcgat ggcgaacggc gacctgacgg
     6181 cgaccacgcc ggtctcgttc tcggccaacg gcctggattg ggcgcagtcg ggttcgatgc
     6241 cgctggccga ggttcaggcg gacgcgacaa ccacctgccg tgagttcgtc accgccctcc
     6301 atggttccac gccatggctg gtcgcgacct cgcgactttc gttcccgtgc agcgaaggcg
     6361 gcttcgtcgt ccatggaaca cagcgggcat caaccgatgg cacgacctgg acagacctgc
     6421 cattcgctgc cggaacccca ggtcagacgg gatcgggctc ggccgtcttc gccaccgtca
     6481 tggtgaatgg ccgccttgtc ctggccggcc agtccaacag cgcggccacg ttctggatcg
     6541 gcgaatagcc ggcgggtcac aggtacagcg gtcgccctcg aattcgaagc ctatgatcgg
     6601 tcggtgacga ccagagccga gccccgatcc ggcccgcgaa ccgtccgcgc gccacgcgga
     6661 acggagctgt cgaccaaggg ctggcagcag gaagccgccc tccggatgct catgaacaac
     6721 ctcgatccgg acgtggccga ggatcccgac cacttgatcg tgtacggcgg caccggacgc
     6781 gcggcacgct cgtgggaggc cttcgacgcc atcgtccgcg agctcagacg gctcgagaac
     6841 gacgagacgc tgctcatcca gagcgggaag ccggtcggcg tctttcgcac caccccggat
     6901 gcgccgaggg tcctcatcgc gaacgcgctc ctcgtccccc actgggcgac ctgggagaac
     6961 ttccgcgagc tcgagcgggc cggcctgacg atgtacggcc agatgaccgc cgggtcgtgg
     7021 atctacatcg cgacccaggg aatcatccag ggcacgtacg agacgttcgc cgagtgcggg
     7081 cggcagcatt tcggcggctc gctgcgcggg acggcgaccc tcaccgcggg gctgggcggg
     7141 atgggtggtg cgcagccgct ggccgtgacc atgaacgagg gtgcggtgct ggccatcgag
     7201 gtcgacccgg cacgggcgga gcgccggcac gtcgccggtt acgtggacga ggtgacgacc
     7261 gatctcgacg acgcccttgc aaaggtggat ggctggcgag cccgcggcga ggcgcggagc
     7321 atcgcgctgg tggccaacgc cgccgacgtg gagccggagc tcgtgcgccg cggctggcgc
     7381 ccggacgtgg tcaccgacca gacatccgcg catgaccccc tcggcggcta cgtgcccggg
     7441 ggcatcggcc tgccagacgc cctggccctc cgcgaagcca gcccggacga gtacgtcgcg
     7501 cgggcgaagg cctccatcgc cgaccacgtg cgggcgatgc tcgccttcca gcaggccggg
     7561 tcggtcgtct tcgactacgg caacaacatc cgcgccttcg cgcgggaagc cggcgtggcc
     7621 gacgccttcg ccatcccggg ctttgtcccg gccttcatcc ggcccctctt ctgcgagggc
     7681 aagggcccgt tccgctgggc cgcgctgtcg ggcgatccga acgacatcct gaagaccgat
     7741 gccgctctcg ccgagctctt cccggacgat gcctccctcc agcgctggct gcgcctcgcc
     7801 gaggggaagg tcccgttcca gggcctgccc gcccggatct gctggctggg ctacggcgag
     7861 cgggcccgag ctggccttcg gatcaacgac atggtccgct ccggcgagct ggccgcgccc
     7921 atcgtcatcg gtcgcgacca cctcgacgcg gggagcgtgg ccagcccgta tcgcgagacg
     7981 gagggcatgc gcgacgggtc ggacgcgatc gcggactggc cggtcctcaa cgccctgctg
     8041 aacacggcgg ccggcgccac ctgggtcagc gtccaccacg gtggtggggt ggggatcggc
     8101 tactcgctcc acgcgggaat ggtcatcgtc gccgacggga ccgatgcggc cgcggcccgg
     8161 ctggaacggg tcctgaccac ggaccccggg atgggcgtca tccgccacgc ggacgcgggc
     8221 tacgagcgcg cgctggaggt cgcccgcgag cgcggtgtac gagtaccgat ggccgagggc
     8281 gaggcctgac ttccggaccc catccgcgca ggtggcgttg ccgcataatc gggacgctct
     8341 gatccactcc cacaaggaga ccttcgtgcg ccatcgctcg cccattgccc tcgtctcggt
     8401 tgccctgttc gtcgccgcct gcgcgagccc gggaaccggt gggatgacct ccgtcttcga
     8461 cctcgccaat ggccagtgct tcaacgacgc cggcgagacg atcgtcgacg aggtcgaggt
     8521 cgtggattgc gcaaacgccc atgactacga gatctacgcc gttgatgact accccgccga
     8581 atcgggcgcc gactacgtgg gcgaagagga gatcgggacc ttcgttcaga ccacctgcct
     8641 ggacggcttc gaagagttcg tggggatgac ctacgaggag tcggagttgg acatctacta
     8701 cctcgccccg accgaggatt cgtggggcag cggtgaccgc gaggtcctct gcgcggcgta
     8761 ccttccgagc gataccggag atccggagcc ggtcgaagga acgctggagg gaagcaaccg
     8821 ctagggcgag accaccaggt ccggcgtaag actcgccggc gaaggggcgc cggccagggc
     8881 catcgcgagc tcgatctccg cggtcagcag gtccagcaca cggcccacgc cggcctcgcc
     8941 gtcgacggcc agcccccata gcgtgggacg gccgatgccg acggccgtgg cgcccaacgc
     9001 gagcgcgatc acgacatcgg agccgcgtcg gacgccaccg tccaggagca ccggaacgcg
     9061 gccgccgacc gcctgcagca ccgccggcag cgcttcaagg ctggcaatcg cgccgtccag
     9121 ctgtcttccg ccgtggttgc tgacctggat ggccgccacg ccgtgctcga ccgccatccg
     9181 cgcgtcgtcg gggtggagga tgcccttgat cacgaccggg cggccgaagc cggtcagcca
     9241 cgccacatcg tcccaggtga tgcgctcgac gaatgggacg ctcagcccgt gctcggtggg
     9301 aacgccggcc gcgaccgcgg ggttcgggac gtggatatcg tcggggatgg cgaaccgatc
     9361 gcggatgtcg cgctcgcgac gcccgatgcg gggcaggtcg acggtcagca ccatcgcctc
     9421 gtagcccgag gcgatcgccc gctcgacgag ggcgcgccgg gcgcctggat cggcgagcag
     9481 gtagacctgg aaccagcgag gcccgccgat ctcggccacc gccgcgaggt cggtgctcga
     9541 cgaggtggac agggtcatcg tgatcccgcg ggccgccgcc gcgcgggcgg tgcccagctc
     9601 cccgtcgggg tgggccatga ggtggaaggc ggtcggacat aggaacaggg gcgagggcca
     9661 gcgacggccg aggacttcca ccatggtgtc gacggtggac acgtcgcgaa ggacgcgggg
     9721 cacgatccat cggcgatcga atgcggcgcg gttctcggtc agcgtccgct cgtcgttcgc
     9781 gccgcctcgg tagtaggcga gcgccccggc cggcaggcgc gcaaccgcgg cctgctcata
     9841 gtcgtggagg ttgatcaggg tcgggtcgtc aaccgtttcg ggctggaatc cggccagcgc
     9901 cggccgcgcc ggctcgctca tcgcgccccg accgcggacc gggaagtctt gcgggtatcc
     9961 accgaccgat ggtagcgcgg gcgggttcgg ggagccgagt ggcgggtagc atcgcgggca
    10021 tgtcgttcga cccctctgcg ccgctccccg gcgcccccga cccgtgggca ggatccgaga
    10081 tgccggcgct gcgcgacggc cccccgtggg cgatgaccga gatgatcgcg tccgagccgg
    10141 cactggccgg ccggctcgtt ccgagactga ccgccgatcc ggcgctcggg cgggtggccg
    10201 acgcggtccg cgaggcggcg gctcgcggcg agacgatcac ggtcactggc tgcggcacga
    10261 gccatcacgc cgccctcgcg atcgcgagcc tcatccgagg tgggttggcc gacaacggtc
    10321 agcggaccga tggcgttcgt gccgctcagg cctttgagct gacgggctcc ccaccaacgc
    10381 ccggggtggt gattgcggtc tcgcacgagg gtgggaccga ggcgacgaac cgatccctcg
    10441 cgcacgccgg cggtcgcgac gcgccgacgg cgctgatcac cgtcagcgac cgctcgcccg
    10501 gggcggccct ggctcgtgac gtgctcacca ctggggagca ggaccagtcg tggtgccata
    10561 cagtgggcta cctctcgccc ctcgtcgtgg gcgcttgctt gcgcgccgcc gtgcgcgggg
    10621 agacgctcag tgcccacgcg gtggccgacc aggtggcggc cggatccgat tcgtccgccg
    10681 cggagggggc cgccgccgca ctggccggcg cggatcggct cctgatcgtg ggctccggcg
    10741 cagactgggc ggccgcc
//
LOCUS       DASYGU010000042        11922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_623233, whole genome shotgun sequence.
ACCESSION   DASYGU010000042 DASYGU010000000
VERSION     DASYGU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623233"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1989)
                     /gene="gyrB"
                     /locus_tag="VGO32_01070"
     CDS             complement(13..1989)
                     /gene="gyrB"
                     /locus_tag="VGO32_01070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV7199374.1"
                     /translation="MTAQPSPPARRRTRASASYTAADIQVLEGLQAVRKRPGMYIGST
                     DARGLHQLVWEVVDNSIDEAMANMATRIDVTIGDDGHLEVVDDGRGIPVDKHSTGKSA
                     LEVIMTVLHAGGKFGGSGYKVSGGLHGVGVSVVNALSSEMRVEVLRDSKRYVQEFRRG
                     APIKPVRQDGGEAEANRGIDWTRHHGTRTRFTPDPEIFEELDFSWETIATRLRESAYL
                     NKGLWIRLVDERTDHEKNFLFQGGVTSFVRHLNRHRVALHQQPIYVERVVDGTSVEVA
                     LQYNDGFAETVLPFANNIHTVDGGTHVTGFRAALTGSLNDWARKASVLSEKDANLSGD
                     DVREGLTAVISVKLTDPQFEGQTKAKLGNADIKGIVQTVVSDGIVQHLEENPADGRRI
                     IEKCVTASRAREAARKARDLVIRKGALDGMALPGKLADCQERDPALSELYIVEGDSAG
                     GSAKQGRDRRNQAILPLRGKLLNVEKARLDKVLSSENVRPLIIALGAGIGEQFDIAKL
                     RYHRICLLSDADVDGAHITTLLLTFFYRHMPEVIENGYLYLCQPPLYRVSTGKQTRYA
                     KDEKEREGALKELSRDGKGRNVTVQRFKGLGEMNPDQLWETTMNPETRTLLQVAVRDA
                     ADADETFSTLMGERVEPRRDFIRGEARKVRNLDI"
     gene            complement(1986..2903)
                     /locus_tag="VGO32_01075"
     CDS             complement(1986..2903)
                     /locus_tag="VGO32_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV7199375.1"
                     /translation="MVDRTPRDFGLGRGLDALIPPPAGAEDGRQVPLNRVVANPHQPR
                     ARFEPDELADLAASISAHGVLQPVVVRGLANGDYQLIAGERRVRAARIAGLTQIPAVI
                     RDPSDEEMVELALVENLQRTDLNPLEEAMGFRLLIDRFGMPHEAVATRVGRSRAAVTN
                     ALRLLDLAPETQEALLDGQISEGHARALAGLTVLELQVAVLAIVIEKGLSVRQTEELV
                     RRRRRQARPDAVRPEHEDVQDLENRLRALLETKVAITRTRRGGRISIEFGSDEELDRL
                     MTWILRNVPERIPTPVPVGAGIESGGDFE"
     gene            complement(2896..3654)
                     /locus_tag="VGO32_01080"
     CDS             complement(2896..3654)
                     /locus_tag="VGO32_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV7199376.1"
                     /translation="MARVTACTNQKGGVGKTTTVINLAAYLALSGIRTLVIDLDPQGN
                     ASTGLGADRRDRGTIYDALINRVAISELARPTAIPGLDLVAATPSLAGAEVELVGIPA
                     REHRLAASLAGVDGEYDRVLVDCPPSLGLLTVNALTAADGVLIPIQAEFYAMEGLGNL
                     VNTIRMIRSSLNPRLEIEGVVITMFDRRTRLSYQVADEVRRHLDGSVYDTVVPRSVRL
                     SEAPSHGLPIALYDPASHGAHAYEALAAEVAARG"
     gene            3889..4359
                     /locus_tag="VGO32_01085"
     CDS             3889..4359
                     /locus_tag="VGO32_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199377.1"
                     /translation="MTGVIHASLAPLLGMGDLRPNLILAAVVTATALFGLGTGAVWAF
                     VAGLTANLLTTDPLGTLPLSLLLVAAMVAAVGRGVGRTGPLLALFGGLVGSFLLDVVG
                     LGGVLLEGGSLPISQVPELARLIIPTAVVNGALAVALFLATRMIVGRFGYEPAT"
     gene            4359..6272
                     /gene="mrdA"
                     /locus_tag="VGO32_01090"
     CDS             4359..6272
                     /gene="mrdA"
                     /locus_tag="VGO32_01090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7199378.1"
                     /translation="MGSEFEHADLPRSGVRFLAFGVITALLFTIIAGRLFQLQVVEGS
                     VRAEQAEAAHAVDVAIPAERGLVFDRLGRPLVVNVPTWTISVRPADLPDGRRVDVLRR
                     VAMVAGVSAADLEDRLDAFSGSPFDLVPLIDDVDRDAALILAEETARLPGIELQVVPR
                     RQYLNERGQVDGTLLANVVGYTGPINREELETLDDEGYLPDDPIGRTGIEAAFESELR
                     GEYGSVRWERDATGRLVKVIEETSQPVAGRNLVLTIDSRLQRIATESLLWGMREANVS
                     QGVTIVMNPQTGEILAMVSLPSYDNNAFAGGISDELYQSYLADPNLPLRNHAIADIYP
                     PGSTFKLVTGLAALEEGVTSPTKRWPTYGCYQIPGAPTGDCLSDWNRQGFGNLNMVEA
                     FAISSDTFFYQMAVHLGVDRLAKWAYELGFGAPTGIDLPSEASGVIASTDWAHSQGRT
                     GVFTGELAQAGIGQNVIAVTPMQLLNAYAALANGGHLMRPQIVLGEADDEGNLVEQFE
                     PEVLHEVAANPANLQTMRIGARQVITTGHAYNISDLRLPGTLSGKTGTAEFGLPDAQG
                     VLPFHSWFVAYVPSRPGATDASLAVLTFTYRATVIGNVSTEVVKYFLQQYFELDQDLR
                     LDPVTLRLLSGGN"
     gene            6276..7409
                     /locus_tag="VGO32_01095"
     CDS             6276..7409
                     /locus_tag="VGO32_01095"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV7199379.1"
                     /translation="MGVAVSTIEVHGSRRWRDFDWQLTLYLLLLIGFGIVLGISAAWN
                     EAGSAGGIPQPVKTVIWTLLGFSLMTVAASVDYHWLRTFAIPIYLFTLGLLVVNLVAG
                     TAISGAQLSVTIAGLDFQFSEISKVLMVVVLAAFLAGRGQRIGRLSTILGAGLLVALP
                     TALVLRQPDLGTALVFVAILGGMLFISGASIGWMALLLGVAVVMTPLALGRLADYQTA
                     RLLCFLDPAADPQGACYQLVQALNAVGSGGWLGQGLTAGDANQLGFLPVQTTDFIFTI
                     VAEELGLAGSLILLALFGLLLWRITIIGWRAKDGLGRLVAMGLATMLLFQVVVNIGMV
                     IGLLPVTGIPLPLITYGGSSTVSLLFGMGLVQSVRMRTRTETF"
     gene            complement(7417..8577)
                     /locus_tag="VGO32_01100"
     CDS             complement(7417..8577)
                     /locus_tag="VGO32_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7199380.1"
                     /translation="MEWVARHRLGLFVAFAIAVVLIALWLARSALPVFGIAIALAFLL
                     DPPVTALQRRRVPRLAGILIVYVVVIVVVVGLGWLLLPPLFEQLQRFLEELPTLASQL
                     MAWEQAIVDWLANLPLPDPIREALDTIIASGQQTVIGLLEAIVGPLLSFVARTAGFIV
                     GLVVVPVWLLYVLKDRERLPDGVLRMLPVGWRTDTAHAMGLTTGLIGRWIRGQILLGA
                     VIFTATLIGLLVLALIGFSEFADFAVLLALVAGILEFVPIIGPIIAAVPAVLIGASIS
                     PAAALAAIALYTVVQQLENHLLVPKVMGDAVDLHPAVVILSLIMGASLFGLWGAILAA
                     PVVALARDLYRYIFRRLEGGTPDEARTFATAGPAPEPAAAGPPPGPSDVAVA"
     gene            8636..9346
                     /locus_tag="VGO32_01105"
     CDS             8636..9346
                     /locus_tag="VGO32_01105"
                     /inference="COORDINATES: protein motif:HMM:NF021610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HEV7199381.1"
                     /translation="MTADADAPRQRWQILYARSEPALRMRQQDITAEFQRVLTEAELP
                     VSQTAAKRPRARIRLAAVTPPSLELKGDVIEVWFDELVARDRLITAGEALADGLSIVD
                     AREVWHGFPSASSQVRGGEYEVEARAPEGIGADEVRAAVVQLLAAPTLPGLRRRGESE
                     RKSDANERDLRPYVEDLELLSFDDAEHVAHLRMQLRLDPSGAGRPRDVVDALDLGLVM
                     TRAIRDRLLFVDTPAIAR"
     gene            9409..9906
                     /gene="rplU"
                     /locus_tag="VGO32_01110"
     CDS             9409..9906
                     /gene="rplU"
                     /locus_tag="VGO32_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV7199382.1"
                     /translation="MYAVVDNGGKQYRVEAGTTLVIDRLDAEAGASVTFDRVLLIVDD
                     DDVTVGTPTVEGAAVHATVVEHGRGPKIIVFKFRPKAHYRRKTGHRSELTKVHIDEIA
                     TASSGRRKAAKAEAAETAPAETPTKAAAKASTKTAATKAATAKPAAPRRKPAAKPTKA
                     APAKD"
     gene            9910..10167
                     /gene="rpmA"
                     /locus_tag="VGO32_01115"
     CDS             9910..10167
                     /gene="rpmA"
                     /locus_tag="VGO32_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV7199383.1"
                     /translation="MAHKKAGSSSKNGRDSAGKRLGVKRGDGQLVMAGTIIVRQRGST
                     FHPGPNVGMGRDYTLFATTEGRVRFTHETRAKKRVSVVAES"
     gene            10171..10377
                     /gene="rpmE"
                     /locus_tag="VGO32_01120"
     CDS             10171..10377
                     /gene="rpmE"
                     /locus_tag="VGO32_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEV7199384.1"
                     /translation="MKPDIHPQYHQAEVHCACGTTFTVGSTAESIRVEVCSNCHPFYT
                     GTQNIVDTAGQVERFQRRMERATR"
     gene            10407..11402
                     /locus_tag="VGO32_01125"
     CDS             10407..11402
                     /locus_tag="VGO32_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEV7199385.1"
                     /translation="MSRLVQHLAFLIPEQLIAAGPFVIEAVPGTTRYRMPDFFYGGQA
                     LIEGVMMRGRSTAAMSVRMPDGEIRTSSEPLPRALSGGRWLKVPFVRGVLVLYETLIL
                     GTRMLMRSAALAAEGEEIEIGRGTLAVTMIISLGFAIGLFFVLPLLLSEVAEEAAGSD
                     LVANLAEGGIRLVIFVIYLALIGLMADVRRVFAYHGAEHKAISAHEADAPLEPESVDR
                     FSTAHTRCGTTFVLIVVVISILFFSVIPRADVPLPLLMLSRIVLVPVVAAVAYELVRF
                     GARHFGNRVVRAIYTPGLWLQSLTTRPPDHEMLEVSISALERVLAEDQAPASAPA"
     gene            11399..>11922
                     /locus_tag="VGO32_01130"
     CDS             11399..>11922
                     /locus_tag="VGO32_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEV7199386.1"
                     /translation="MTDQLDELVARHQELTDLMSRPETATVPGQLAKYGKELARLDPI
                     VAALREHEANARSLTSARGLLEDGDPEMRSMARDEIASLEARQAKLAAELRRLLVPRD
                     PNDERDVILEIRAGTGGDEASLFAADLYRMYGLYAERRGWQVELLSTSEGGSGGLKEV
                     IAEVNGDAAYSRLK"
BASE COUNT         1838 a         4046 c         4069 g         1969 t
ORIGIN      
        1 tgggaatccc tctcagatat cgaggttgcg gaccttgcgg gcctcgcctc gaatgaagtc
       61 acggcgcggt tccactcgct cgcccatgag cgtgctgaag gtctcgtccg cgtccgccgc
      121 gtctcggacg gcgacctgga ggagggtgcg ggtttcgggg ttcatcgtgg tttcccacag
      181 ctggtcggga ttcatctcac ccagaccctt gaatcgctgc acggtgacgt tgcgaccctt
      241 tccgtccctg gacagctcct tgagcgcacc ctcccgctcc ttctcgtcct tcgcgtagcg
      301 agtctgcttc ccggtgctga cccgatagag cggtggctgg cagaggtaca ggtacccgtt
      361 ctcgatgacc tcgggcatat gccgatagaa gaaggtcagg agcagggtcg tgatatgcgc
      421 accgtcgaca tcggcgtccg acaggaggca gatccggtgg tagcggagct tggcgatgtc
      481 gaactgctca ccgatgccgg cgccgagggc aatgatcagc ggccggacgt tctcgctgga
      541 caggaccttg tccagccgcg ccttctcgac gttcagcagc tttccgcgga ggggcaggat
      601 ggcctggttt cgacggtccc gtccctgctt ggccgagccg ccggccgagt ctccctccac
      661 gatgtacagc tcgctcaggg ccgggtctcg ctcctggcag tcggcgagct tgccggggag
      721 ggccatcccg tccagggcac ccttgcggat gaccaggtcg cgagccttgc gcgccgcttc
      781 acgggcacgc gacgcggtga cgcatttctc gatgatccgc cggccgtccg cgggattctc
      841 ctcgaggtgc tggacgatcc cgtccgagac cacggtctgg acgatgccct tgatgtccgc
      901 gttgccgagc ttggccttgg tctggccctc gaactgggga tcggtgagct tgacgctgat
      961 cacggcggtg aggccctcgc ggacgtcgtc gccgctgagg ttggcgtcct tctcggacag
     1021 gacgctggcc tttcgcgccc agtcgttcag ggagccggtc agggccgcac ggaatccggt
     1081 cacgtgcgtc ccgccgtcga cggtgtggat gttgttggcg aacggcagga ccgtctccgc
     1141 gaagccgtcg ttgtactgga gggcgacttc gacgctcgtg ccatccacca cccgctcgac
     1201 atagatcggc tgctggtgca gcgccacccg gtggcggttc aggtggcgga cgaaggaggt
     1261 gactccgccc tggaagagga agttcttctc gtgatcggtc cgctcgtcca cgaggcggat
     1321 ccacagcccc ttgttcaggt aggccgactc ccgcagacgg gtcgcgatcg tctcccagct
     1381 gaagtcgagc tcctcgaaga tctcggggtc cggggtgaat cgggttcggg tcccgtgatg
     1441 gcgggtccag tcgatgcccc ggttggcttc cgcctcgccg ccgtcctgcc ggaccggctt
     1501 gatcggtgcg ccgcggcgga actcctgcac gtaccgtttg ctgtcgcgca gcacttcgac
     1561 gcgcatctcg gaggacagcg cattgactac gctcacgccc acgccgtgga ggccgccaga
     1621 caccttgtat ccgctcccgc cgaacttccc gcccgcgtga agcacggtca tgatcacttc
     1681 gagcgcgctc tttccggtcg agtgcttgtc gaccggaatg ccccgaccgt cgtcgacgac
     1741 ttccaggtgg ccgtcatctc cgatcgtcac gtcgatccgg gtggccatgt tggccatcgc
     1801 ctcgtcgatg ctgttgtcca cgacctccca gacgagctgg tggaggccac gggcatcggt
     1861 cgagccgatg tacatgccgg ggcgcttgcg gacggcctgc agcccttcga ggacctggat
     1921 gtcggccgcg gtgtacgagg ccgatgcgcg cgtgcggcgc ctggcgggcg ggctgggctg
     1981 ggcggtcatt cgaagtcacc cccggactcg atcccggccc cgacgggcac cggtgtgggg
     2041 atgcgctccg ggacattgcg caggatccag gtcatcaggc ggtcgagctc ctcgtccgat
     2101 ccgaactcga tgctgatccg gccgcctcgt cgcgtccgcg tgatggcgac cttggtctcg
     2161 agcagggcac ggaggcggtt ttcgagatcc tggacgtcct cgtgctccgg gcgcaccgcg
     2221 tccggtcgtg cctggcgccg acggcgtcgg acgagctctt cggtctggcg gaccgagagg
     2281 cccttctcga tcacgatcgc aagcaccgcg acctgcagct cgagcacggt gagccccgcg
     2341 agcgcgcggg catggccctc gctgatctgc ccgtcgagga gcgcctcctg cgtctcgggt
     2401 gccagatcga ggagtcgcag ggcattggtc acggcagccc tcgagcggcc gacgcgggtc
     2461 gcgaccgcct cgtgtggcat cccgaaccgg tcgatcagga gtcggaagcc catcgcctcc
     2521 tcgagtgggt tgagatcggt ccgctggagg ttttcgacga gggccagctc caccatctct
     2581 tcgtcgctcg gatcccgaat cacggccgga atctgggtca ggccggcgat tcgggcggcg
     2641 cgaactcgac gctcgcccgc gatcagctgg taatcaccgt tggccaggcc gcggacgacc
     2701 accggctgga gcacgccgtg ggcggaaatg gatgcggcga ggtcggcaag ctcatccggc
     2761 tcgaaccggg cgcgcggctg gtgtgggttg gcaaccaccc ggttcaaggg gacctggcgt
     2821 ccgtcctcgg cgccggcagg cggcgggatg agcgcatcga gaccgcgtcc cagcccgaag
     2881 tcgcgcggcg tgcggtcaac cacgagcggc gacctccgcc gccagtgcct cgtacgcatg
     2941 agccccatgc gacgccgggt cgtagagcgc gatcgggaga ccatggctcg gggcctcgct
     3001 cagccgaacg ctgcgtggta cgacggtgtc atacacggag ccatccagat gacgccggac
     3061 ttcgtccgca acctggtacg agagccgggt ccggcggtcg aacatcgtga tcacgacccc
     3121 ctcgatttcg agacgcgggt tgagggagct gcggatcatc cggatcgtgt tcaccaggtt
     3181 tcccagtccc tccattgcgt agaactcggc ctggatcggg atcaggaccc catcggcagc
     3241 cgtgagcgcg ttcaccgtga gcaggccgag tgacggcgga cagtccacca gcacccgatc
     3301 gtattccccg tcgaccccag ccaggctcgc cgccaggcgg tgctccctcg ccgggatccc
     3361 gaccagctcc acctcggcgc cggccaaact cggtgtggca gctaccaggt cgagccccgg
     3421 gatggccgtc ggccgagcca gctcggagat tgcgacccgg ttgatcaaag cgtcgtagat
     3481 cgtgccccga tctcgacgat cggctccgag cccggtcgag gcattgccct ggggatccag
     3541 gtcgatgacc agagtacgga tgcccgagag cgccaggtac gccgcgagat tgatgacggt
     3601 ggtcgtctta ccgacgccgc ccttctggtt ggtacaggcc gtgaccctag ccacgaacac
     3661 tccgttgcga gttcctgtgg gcccgtcaat tgtaccagtt tggccccctt tcagcctgtt
     3721 accagcggct caacgtttca cgtgaaacat gccactttgg tcctgcgttg agttcgaggc
     3781 gaccccaatc gggacctcca cccgccctat actgccggga gccgcccagt agtccgcagc
     3841 gaccccgaga atcgaccgat aaacgtcatc agcgcgcttc ttgccttcgt gactggcgta
     3901 atccatgcca gcctcgcccc gctgctgggc atgggagatc tgagacccaa tctcatcttg
     3961 gcggcggttg tcaccgcgac cgcgctgttc ggcctcggta ccggagcggt ctgggccttc
     4021 gtcgccggct tgaccgccaa cctcctcacg accgacccat tgggcacgct gccgctcagc
     4081 cttctgctcg tggccgcgat ggtggccgcg gtcgggcgcg gcgtcgggag gaccggcccg
     4141 ctgctcgccc tgttcggcgg cctggtgggt agcttcctgc tcgacgttgt cgggctgggg
     4201 ggcgttctgc tcgagggtgg atcgcttccg atcagccagg ttcccgagct cgctcgtctg
     4261 atcatcccca ccgcagtcgt caacggggcg ctggcggtcg ccttgttcct ggcgacccgg
     4321 atgatcgtgg gtcggttcgg ctatgagccc gcgacctgat gggatcggaa ttcgagcacg
     4381 ccgacctgcc gcggtccggg gtccgcttcc tggcgttcgg ggtcatcacc gcgctgctgt
     4441 tcaccatcat cgccgggcgc ctgttccagc tccaggtggt cgaaggctcg gtcagggccg
     4501 agcaggccga ggcagcccac gcggtcgacg tggcgatccc cgccgagcga ggactggtct
     4561 tcgaccggtt gggtcgaccg ctggtcgtca acgtgcctac ctggaccatc tcggtccggc
     4621 ccgcggacct gccagatggg cgccgggtcg atgttctccg tcgcgtggcg atggtggctg
     4681 gcgtcagtgc ggccgacctc gaggatcggc tcgacgcgtt cagtgggtcc ccgttcgacc
     4741 tcgttccgct gatcgacgac gtcgaccgag atgccgcgct gatcctggcc gaggagaccg
     4801 cacggctgcc ggggatcgag cttcaggtcg tgccgcgccg gcagtacctg aacgaacggg
     4861 gacaggtgga cggcacgctg ctcgccaatg tggtggggta caccgggcct atcaaccgcg
     4921 aggagctcga gacgctcgac gacgaggggt accttcccga cgaccccatc ggccggaccg
     4981 gaatcgaggc cgcgttcgag tcggagcttc gcggggagta cgggtcggtg cgctgggagc
     5041 gggacgcgac cggccggttg gtcaaggtca tcgaggagac gagccaacct gtcgcggggc
     5101 gcaacctggt gctgaccatt gactcccgcc tgcagcggat cgcgaccgag tccctgctgt
     5161 ggggcatgcg cgaggccaac gtcagccagg gcgtcacgat cgtcatgaat ccccagaccg
     5221 gcgagatcct ggccatggtc tcgctccctt cgtacgacaa caacgcattc gcggggggca
     5281 tctccgacga gctgtaccag tcgtacctcg ccgatccgaa tctcccgctg cgcaaccacg
     5341 cgattgccga catctacccg ccgggctcga cgttcaagct ggtcaccggg ctcgccgcgc
     5401 tggaagaggg ggtgacctcg cccaccaagc gttggccgac ctacggctgc taccagatcc
     5461 ccggggcgcc gaccggagac tgtctgagcg actggaaccg tcagggcttc ggcaacctca
     5521 acatggtcga ggcgttcgcc atcagctcgg acacgttctt ctaccagatg gccgttcatc
     5581 tgggcgtcga ccggctggcg aagtgggcct atgagctcgg gttcggggcg ccgaccggta
     5641 tcgacctccc cagtgaggcg agcggcgtga tcgcctccac cgactgggcc cacagccagg
     5701 gacggaccgg cgtcttcacc ggcgagctgg cccaggcggg gatcggccag aacgtcatcg
     5761 cggtcacccc gatgcagctc ctgaacgcgt acgcggcgct cgcgaacggc ggccacctga
     5821 tgcgaccgca gatcgtcctt ggcgaggctg acgacgaggg caatcttgtc gaacagttcg
     5881 agcccgaggt cctgcacgag gtggccgcca acccggccaa cctgcagacg atgcggatcg
     5941 gggctcggca ggtcatcacc accggtcacg cctacaacat cagtgacctc cggctccccg
     6001 gcaccctgtc cggaaagacg ggcaccgcgg agttcgggct gccggacgcc cagggcgtgc
     6061 tgccgttcca ctcatggttc gtggcgtacg tgccctccag gccgggcgca accgacgcct
     6121 cgctcgcggt gctcaccttc acctaccgcg cgacggtcat cggcaacgtg tccaccgagg
     6181 tcgtgaagta cttcctgcag cagtatttcg agctcgacca ggatctccgc ctggatccag
     6241 tcactctgcg gctgctgagc ggcggcaact gaccgatggg cgtcgcggtt tccaccatcg
     6301 aggtccacgg atcacgccgc tggcgcgatt tcgactggca gttgacgctg tacctgctgc
     6361 tcctcatcgg cttcgggatc gtgctcggaa tctcggcggc ctggaacgag gccgggtccg
     6421 cggggggtat cccccagccg gtgaagacgg tcatctggac ccttctcgga ttcagcctga
     6481 tgacggtggc ggcgtcggtc gactaccact ggctgcggac gtttgcgatc ccgatctacc
     6541 tgttcaccct gggcctgctg gtcgtaaacc tcgtggccgg cacggcgatc tcgggcgccc
     6601 agctgtcggt cacgatcgca ggcctggact tccagttctc cgagatctcg aaggtcctca
     6661 tggtcgtcgt gctcgcagcg tttctggcgg gtcgaggaca gcggatcggc cggctgtcca
     6721 ccatcctggg cgccgggctg ctggtcgccc tgccgacggc cctggtcctc cgccaaccgg
     6781 acctggggac cgcgctcgtg ttcgtcgcga tcctgggcgg gatgctgttc atcagcgggg
     6841 cgagcatcgg ctggatggcg ctcctgctcg gggtggcggt ggtgatgacc ccgctcgcgc
     6901 tcggccgact tgcggactac cagacggccc gcctcctctg cttccttgac cccgccgcgg
     6961 atccgcaggg tgcgtgctac cagctcgtcc aggcgctgaa cgccgtcggg tcgggcggct
     7021 ggctgggcca gggcctgacc gcgggcgatg ccaaccagct gggattcctg ccagtccaga
     7081 ccaccgactt catcttcacc atcgtcgccg aggagctggg cctggctggc agcctcatcc
     7141 tcctcgccct gttcgggctt ctgctgtggc ggatcacgat catcggttgg cgggcgaagg
     7201 acgggctcgg ccggctcgtg gcgatgggcc tggccaccat gctcctgttc caggtggtcg
     7261 tgaacatcgg gatggtgatc gggctgctgc cggtgaccgg catcccgttg ccgctgatca
     7321 cctacggcgg ctcgtccacc gtgtccctgc tgttcggtat gggccttgtt caatcggtcc
     7381 ggatgcgtac ccgcaccgag acgttctagt cgagcatcag gcgacggcca cgtcagaggg
     7441 gcccggcggc ggaccggccg ccgcgggttc cggcgcgggt ccggcggtcg cgaaggtccg
     7501 ggcttcgtcg ggcgtcccgc cctcgagccg gcggaagata tagcggtaca ggtcacgggc
     7561 gagggcgacc accggtgcgg cgaggatcgc gccccagaga ccgaacaggc tcgcgcccat
     7621 gatcagtgac aggatgacca ccgccgggtg gagatccacc gcgtcgccca tcaccttggg
     7681 cacgaggaga tggttctcca gctgctgcac gaccgtgtac agggcaatcg ccgccaacgc
     7741 cgcagccggg gagatcgagg ctccgatcag gacggccggg acggcggcga tgatcggacc
     7801 gatgatcggc acgaactcga ggatgccggc cacgagcgcg aggagcaccg cgaagtcggc
     7861 gaactcgctg aacccgatga gggcgagcac caggagaccg atgagcgtgg cggtgaagat
     7921 caccgccccg agcaggatct ggccccggat ccagcggccg atgagcccgg tggtcaggcc
     7981 catagcgtgg gcggtatccg tgcgccagcc gaccggcagc atgcggagga cgccgtcggg
     8041 caggcgctcg cggtccttga gcacgtagag cagccagacc gggaccacga ccagcccgac
     8101 gatgaaaccg gcggtccgcg cgacgaagct caggagcgga cccacgatcg cctccaagag
     8161 accgatcacc gtctgctggc cgctggcaat gatcgtgtcc agcgcttcgc ggatggggtc
     8221 ggggagcggg aggttcgcca gccagtcgac gatcgcctgc tcccaggcca tgagctgaga
     8281 tgcgagggtc gggagctcct cgaggaatcg ctggagctgc tcgaacagcg gcggcaggag
     8341 cagccagccg aggccgacca cgaccacgat cacgacgacg tagacgatga ggatcccggc
     8401 caggcgggga acgcgtcgtc gctggagggc ggtgaccggc gggtcgagga ggaaggcgag
     8461 ggcgattgcg atcccgaaca ccggcaacgc agagcgggcg agccacaggg cgatcaggac
     8521 gaccgcgatg gcgaaggcca cgaacaggcc gaggcggtgg cgcgccaccc attccatggc
     8581 gcgatcctaa ccgagcggct tgccgggaac cgggagcggg taccatcggg cgcatatgac
     8641 cgccgatgcc gacgccccgc gccagcggtg gcagatcctc tatgcacgca gcgagcccgc
     8701 gctccgcatg cggcagcagg acatcaccgc cgagttccag cgggtgctga cggaggctga
     8761 gctgcccgtc tcgcagacgg ccgccaagcg gccccgcgcc cgcattcggc tggcggcggt
     8821 cactccgccc agtctcgagc tcaagggcga tgtcatcgag gtctggttcg acgagctggt
     8881 ggcccgggac cgcctgatca cggccggcga ggcgctcgcg gacggcttgt ccatcgtgga
     8941 cgcgcgggag gtatggcacg gattcccgtc agcctcatcc caggtgcgcg gcggcgagta
     9001 cgaagtcgaa gcccgcgccc cggagggaat cggcgccgac gaggtgcgtg ccgcggtcgt
     9061 ccagctgctg gcggccccca ccctgcccgg cctccgccgg cgcggagaga gcgagcggaa
     9121 gtccgacgcc aacgaacggg acctgagacc atacgtcgag gacctcgagc tgctcagctt
     9181 cgacgatgcg gagcacgtcg cgcacctccg catgcagctt cggctcgacc ccagcggtgc
     9241 cggccggccg cgcgacgtgg tggacgccct cgaccttgga ctggtcatga cccgggcgat
     9301 ccgcgaccgc ctcctcttcg ttgacacccc tgcgatcgcc cgataaactc cgggatcccg
     9361 tgggactccc tgtggcgtcc cgctgtgcgc gaaaagacgg agactgccat gtacgccgtg
     9421 gtcgataacg gcggaaagca atatcgggtc gaggccggca cgacgctggt catcgatcgc
     9481 cttgacgccg aggctggtgc ctcggtgacg ttcgaccgcg tcctgctcat cgtcgacgac
     9541 gatgatgtga cggttggaac cccgacggtc gaaggcgccg cggtccatgc gacggtggtt
     9601 gagcatgggc ggggacccaa gatcatcgtg ttcaagttcc gccccaaggc gcactaccgg
     9661 cgcaagaccg gtcaccgctc ggagctgacc aaggtccaca tcgacgagat tgcgaccgcc
     9721 tccagcgggc gtcgaaaggc tgccaaggcc gaagcggccg agaccgcccc ggccgagacg
     9781 ccaactaagg cagcggcgaa ggcatcaacg aagacggcgg ccacgaaggc agcgacggcc
     9841 aagcccgcag cgccccgacg aaagccggcg gccaagccca ccaaagccgc tccggcgaag
     9901 gactgatcaa tggcgcacaa gaaggcaggc tcatcctcga agaacgggcg cgacagcgcc
     9961 ggcaagcgcc tgggcgtgaa gcgtggcgac ggccagctgg tcatggccgg gacgatcatc
    10021 gtccgccagc gcgggagcac gttccacccc ggccccaacg tcgggatggg ccgtgactac
    10081 accctgttcg cgaccaccga agggcgcgtt cgcttcaccc acgagacgcg cgccaagaag
    10141 cgcgtcagcg tggtcgcgga gtcctgaccg atgaagcccg atattcatcc gcagtaccat
    10201 caggccgagg tgcactgcgc ttgcgggacc accttcaccg tgggatcgac cgccgagtcg
    10261 atccgggtcg aggtttgctc gaattgccac ccgttctaca ccggcactca gaacatcgtc
    10321 gacaccgcgg gccaggtgga gcggttccag cggcggatgg agcgcgccac gcgctgaccc
    10381 ggtctgaccg accgaagccg ggtcgcatgt cgcggctcgt ccagcacctc gccttcctca
    10441 ttcccgagca gctgatcgcg gctgggccgt tcgtcatcga ggccgtgccc gggacgacgc
    10501 gctaccggat gccggacttc ttctacgggg gccaggcgct catcgaaggc gtcatgatgc
    10561 gcggccgttc gacggcggcc atgagcgtgc ggatgccgga cggggagatc cgcacctcct
    10621 ccgagcccct gcccagggcg ctcagcggcg gccgctggct gaaggttccc ttcgtgcgcg
    10681 gcgtcctcgt cctgtacgag accctgatct tgggcacccg gatgctcatg cggtcggcgg
    10741 ccctcgccgc cgagggggag gagatcgaga tcggccgcgg gaccctggcc gtgacgatga
    10801 tcatttctct cggcttcgcc atcggcctct tcttcgtcct tccgctgctc ctgtcggagg
    10861 tcgccgaaga ggcggcgggc agcgacctgg tggccaacct ggccgagggt gggatccgcc
    10921 tcgtcatctt cgtgatctac ctggccctca ttgggctgat ggccgacgtg cgccgcgtgt
    10981 tcgcatacca cggcgcggag cacaaggcga tcagcgcgca cgaggccgat gcgccgctcg
    11041 agccggagtc ggtcgaccgt ttcagcaccg cccacacccg ctgcgggacg acgttcgtcc
    11101 tgatcgtggt cgtgatcagc atcctgttct tctcggtcat cccgcgcgcc gacgtcccgc
    11161 tgccgctgct catgctctcg cgtatcgtcc tcgtcccggt cgtggcggcc gtcgcctacg
    11221 agctcgtccg cttcggcgcg cggcactttg gcaaccgcgt ggtccgcgcg atctacacgc
    11281 cgggcctctg gctccagtcg ctgaccaccc gaccgcccga ccacgaaatg ctcgaggtca
    11341 gcatcagcgc cctggagcgg gtgctcgccg aggaccaggc accggccagc gcgccggcat
    11401 gaccgatcag ctggacgagc tcgtcgcccg tcaccaggag ctgaccgatc tcatgtcccg
    11461 ccccgagacg gcgaccgttc ctgggcagct ggcgaaatac ggcaaggagc tcgcacggct
    11521 ggacccgatc gtcgccgcgc tccgcgagca cgaggccaat gcccgatcgc tgacgtcggc
    11581 gcgggggctg ctggaggacg gcgatccgga gatgcggtcc atggcccgcg acgagatcgc
    11641 ctccctggaa gcgcggcagg ccaagctcgc ggccgagctt cgtcggctgc tcgtgccccg
    11701 cgaccccaac gacgaacggg acgtgatcct cgagatccgg gccgggaccg gcggggacga
    11761 agcctcgctg ttcgccgccg acctgtaccg gatgtatggc ctctacgccg aacgacgcgg
    11821 ctggcaggtc gagctgctgt cgacctcgga gggcgggtcc ggcggtctca aggaggtcat
    11881 cgccgaggtc aatggcgacg cggcgtattc gcggttgaag tt
//
LOCUS       DASYGU010000043         6789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1189233, whole genome shotgun sequence.
ACCESSION   DASYGU010000043 DASYGU010000000
VERSION     DASYGU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189233"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VGO32_01135"
     CDS             complement(<1..1267)
                     /locus_tag="VGO32_01135"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEV7199387.1"
                     /translation="MPLVRPLRGLGYALDRYGATEIPARVRRPGEPAAHPGRVADLTD
                     LVSPPYDVISDSQRATLLGRDPRNAVRLELSAEAAPHKAAAATLTSWREDGTLTVRSR
                     AAFYAYRYGAPETAADEPAEVRGILARIRLEPWGPGIRPHEHTMPGPKADRLGLLHAT
                     RTQLSPILVLYIDQSDRSDGLVERQVFEEWRARDGDGLLHRAGVIDDDQGLAAYLSRR
                     KLVVADGHHRYETALAYQADVRASGQARAAAPGSLAADWVMAVLVNAAREHLEIRPTH
                     RLLRKVDAERLRSLVHGPDPLFQAVPVAPEALGERMADLRDAEEAVFGLVLPDSEGWL
                     VIGDVDAAADRMRRELGSAPARTLDLALLHAAVLGDRLGISAADVAAGEMLLYTRSEA
                     DARGRVARGEVAAAILVRPTRLEELTAVAD"
     gene            complement(1277..3493)
                     /locus_tag="VGO32_01140"
     CDS             complement(1277..3493)
                     /locus_tag="VGO32_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199388.1"
                     /translation="MADRPVARFRRVLVGAVVATTLVLSAVGPVAAVDPIEGIPLPEE
                     PAAVQLAEIATEAQSFDLDGDGADELVAVVARDDAGGLAAIQAWSVEADGAVGPTNKV
                     PLRRSASVDELLSGRGRLGIDRDDMIAMRLTEPAKFLVVRRDGSDVLLVAAMGTETDI
                     DAPIPCCLTVWEVVLDDAGSLDLLLVADTQRHAVQVAAVDFDDDDTDELLVTEGLPTS
                     SAGASLLRWDGVDRFGRAGFEVTSIAGCCFVADVADTDGLPGEEALLLSFPEDGTTSL
                     HRLSVRASTIGIEDAALDEVGSARALPLASGPAIVTADFSSQALTVWSWPAGGRPQQI
                     ARRVTGGQIAAVLGTGADTRIVIFADPSSGSILVVPGNLGGGSGPTATVSRDSRASIF
                     ASNGVQLSDSILPTPFFGVLPDGLPNAPDVFVFGGTRITPDPDPEGLIAAAPLALLPD
                     TVPVARVGPDGGWTAIVSRTPDFAIFASGRPEAAEDMLTPTIAGPLHLASTASLLEVE
                     DDLGRLTPTFMGVAPDVDHPPILMVGNEAVDVEIHGPPGTLVRWVIRSVEESASVAAD
                     GVARIRLMEPAGPDAPDGSGSNVGIQVVTPSGHAYSGSWRINVYRQPPDLGIRDQPSL
                     VDFNPTLTGRTLPGSTLTINGIAVPIAGDGSFTVPVEVGILPTELRVVVTDPVGNRTE
                     RIITRVWPVDYRQLPFVPIAVLLTLAAGAILYLRKPDAKPGRGRPDDGATFEEIGG"
     gene            complement(3486..5471)
                     /locus_tag="VGO32_01145"
     CDS             complement(3486..5471)
                     /locus_tag="VGO32_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7199389.1"
                     /translation="MAVRARGPIFGTDSAAAAWRPSAEYLDRSRLRRFLTDVGCADLE
                     ELQERAEADPAWFWSAAVADIGVRFDPKPMAALDVSHGPEWARWWVGAGFNWVDAGVD
                     QPAGDAPDETALAWEGEDGSVRRYSRAALRADVDRAARAMAALGVGRDDRVGVFMPMI
                     PETVVAVLAVGKLGAVYTPIFSGYGAPAVASRLADCGARLLVTADGFWRRGKAVDLKA
                     VADAALAGAPTVQHSLVVRRLGDVAPRAEWTAGRDVAWDEALASAGDEPLRSVRTDAN
                     DPYMLIYTSGTTGKPKGAVHVHGGFPLKSAVDLAHCFDLQRGDTLFWFTDMGWMMGPW
                     AVAGALLLGATLVIYEGTPDHPGPDRLWDLVERHRITHLGISPTAVRALMTHGSEPVA
                     AHDRSSLRVLGSTGEPWNPDPWWWLFRAVGEERVPIINYSGGTEISGGILGCTTITPI
                     APCSFAGPCPGMAADVVDADGLPVRGEVGELAIRQPWPGMTQGFWRDPDRYLEAYWSR
                     LPGTWVHGDWARIDPDGFWYISGRSDDTIKVAGKRIGPAEVESAAVAHPLVVEALAVG
                     VPDEVKGESIVVFAVVRSSAEASDALATAIGQTVVDGLGKSFKPAAVHLVPGLPKTRN
                     GKILRRVARAAYLGMDPGDISALEDAATLEPIRAAHG"
     gene            complement(5504..6655)
                     /locus_tag="VGO32_01150"
     CDS             complement(5504..6655)
                     /locus_tag="VGO32_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7199390.1"
                     /translation="MDFALSSAHEDIRRTVRDFADRRIAPQADEMEVRGEFPAELVRE
                     AASLGLLGVPYPVELGGTGLDNLAYAITIEELSRVSGSMGIIVSAHTSLGAGPLYLAG
                     TPDQQERFLRPLASGEKLGAYGLTEPGAGSDSRGTKTRARRDGGDWVIDGAKQFITNA
                     GVADIYIVVARTGEAEDDSQITAFIVEKGTPGFTFGRLEEKMGLHASATGELLFADCR
                     VPADNLLGDEGEGDKLFLRTLDGGRIGIGAMALGLAQAAYEAASAYAKERKQFGRAIG
                     SFQGVAFKIADMATEIDAARLLIYRAAWLKDQGKPFTTEAAMAKLYASEVAARATNAA
                     IQVHGGYGYVREYKVERYLRDAKLTEIGEGTSEIQRLVIARNLLGIRAM"
BASE COUNT         1014 a         2449 c         2374 g          952 t
ORIGIN      
        1 catcggccac cgcggtcagt tcctccaacc gggtcgggcg caccaagatc gcggccgcca
       61 cctcgccccg ggctacacga ccgcgggcat cggcctcgct cctagtgtag aggagcatct
      121 ccccggcggc cacgtccgcc gccgagatgc cgaggcggtc ccccagcacc gcggcgtgca
      181 ggagcgcgag gtccagcgtt cgggccggtg cggacccgag ctcgcgtcgc attcggtcgg
      241 ccgcggcgtc cacgtcgccg atgaccagcc acccctcgct gtcgggcagg accagcccga
      301 acacggcctc ctcggcgtcg cgaaggtccg ccatccgctc ccccaaggcc tccggcgcga
      361 cggggacggc ctggaacaac ggatccgggc cgtggacgag ggaccgcagc cgctccgcgt
      421 ccaccttccg caggagccga tgagtgggcc ggatctcgag atgctcccgc gccgcgttga
      481 cgaggacggc catgacccag tcggcggcca gcgatccggg agcggcggcg cgtgcctgcc
      541 cggaagcccg gacatcggcc tggtaggcga gcgcggtctc ataccgatgg tgcccgtcgg
      601 ccaccaccaa tttccggcgc gacagatagg cggcgagccc ctggtcgtcg tcgatgacgc
      661 cggcccgatg gaggagcccg tcgccgtccc gcgcccgcca ttcctcgaac acctggcgtt
      721 cgaccaggcc gtccgatcgg tccgattggt cgatgtacag gaccaggatc gggctgagct
      781 gggtccgggt ggcgtgcagg aggcccagcc ggtcggcctt ggggccgggc atcgtgtgct
      841 cgtgaggccg gatgcccggc ccccatggct cgaggcgaat gcgagccagg atgccgcgca
      901 cctcggcggg ctcgtcggcg gcggtctccg gtgcgccgta ccgataggcg tagaacgccg
      961 cgcgcgatcg caccgtcagc gtgccgtctt cgcgccacga ggtcagggtc gcggcggccg
     1021 ccttgtgcgg tgcggcttct gcactgagct cgagccgcac ggcgttgcgt ggatcgcggc
     1081 ccaggagcgt ggctcgttgg gagtcgctga tgacgtcgta tggggggctg acgagatcgg
     1141 tcaggtcggc cacacgaccc ggatgagcgg ccggctcccc cggacgacgg acccgggcgg
     1201 gaatctcggt ggcgccgtac cggtcgaggg cgtagccgag cccgcggagc gggcgcacca
     1261 ggggcatgga ccgatgttag ccgccgatct cctcgaacgt ggctccgtcg tccggccgcc
     1321 cgcgaccggg cttcgcatcg ggcttgcgga ggtacaggat tgcgcccgcg gccagcgtca
     1381 gcagcaccgc gatcgggacg aacggcagct gccggtaatc gaccggccag acccgggtga
     1441 tgatccgctc ggtgcggttg ccgaccggat cggtgactac gacccgcagc tcggtgggaa
     1501 ggatccccac ctcgacgggg accgtgaacg acccgtcccc ggcgatcggg accgcgatcc
     1561 cgttgatcgt cagcgtcgat ccgggaagcg tgcggccggt cagggtgggg ttgaaatcca
     1621 ccaggctcgg ctggtcgcgg atgcccaggt ccgggggctg gcggtagacg ttgatccgcc
     1681 atgacccgga gtacgcgtgg ccactgggag tgacgacctg gatcccgaca ttggagccgg
     1741 agccgtccgg ggcatcgggg ccggctggct ccatcaggcg aatgcgggca acgccgtcgg
     1801 cggccacgct cgccgattcc tccacggatc gaatcaccca cctcacgagc gttccgggtg
     1861 gcccgtgaat ctccacgtcc acggcctcgt tgccgaccat caggatcggc gggtgatcca
     1921 cgtccggcgc aacgcccatg aaggtcgggg tcaggcgacc gaggtcatcc tccacctcca
     1981 gcagggaggc ggtcgaggcg aggtggagcg gccccgcaat cgtgggagtc agcatgtcct
     2041 ccgcggcctc gggccggccc gaggcgaata tggcgaagtc gggcgtgcgc gacacgatcg
     2101 ccgtccagcc gccgtccggg ccgacccgcg ccaccggaac cgtgtccggg aggagggcaa
     2161 ggggcgcggc cgcgatcagg ccctccggat ccggatcggg cgtgatcctc gtgcccccga
     2221 acacgaacac gtccggcgcg ttcggaaggc cgtcgggcag gacgccgaag aagggcgtgg
     2281 ggaggatgga gtcgctgagt tggacgccat tcgacgcgaa gatcgaggcg cggctgtcac
     2341 ggctgacggt ggccgtcggc ccgctgccgc caccgagatt gcccgggacg acgaggatcg
     2401 accccgaaga tgggtccgca aagatgacga tccgggtatc ggcaccggtg ccaagaacgg
     2461 cggcgatctg tccgcccgtc acgcggcgag cgatctgctg aggtcggccg ccagcgggcc
     2521 acgaccagac ggtgagcgcc tgggaggaga agtccgccgt cacgatcgcc ggcccggacg
     2581 ccaggggcag ggctcgggcg gacccgacct cgtccagcgc ggcgtcctcg atgccaatcg
     2641 ttgaagcacg cacgctcagc cgatggaggc tcgtcgtccc gtcctccggg aatgagagca
     2701 gcaacgcctc ctccccgggc aggccatcgg tatccgcgac atcggccacg aagcagcatc
     2761 cggcgattga tgtgacctcg aagccggccc gcccgaatcg gtccacgcca tcccagcgca
     2821 gcaggctggc gccggccgat gacgtgggca gcccttcggt gacgagcagc tcgtccgtgt
     2881 cgtcgtcatc gaagtcgacg gctgccacct gcaccgcgtg gcgctgcgta tccgcgacca
     2941 gcaggaggtc aagcgagccg gcgtcgtcga gcaccacctc ccagaccgtc aggcagcacg
     3001 ggatgggcgc gtcgatgtcg gtctcggtcc ccatggcggc aacgagcagg acgtcgctgc
     3061 cgtcgcgccg gacgacgagg aacttcgccg gctcggtcag gcgcatggcg atcatgtcgt
     3121 cccggtcgat gcccagccgc ccgcgaccgg acagcagctc atcgacgctc gccgagcgtc
     3181 ggagcggcac cttattggtt ggtccgaccg cgccatcagc ctctaccgac caggcctgga
     3241 ttgcggccag gccgccggca tcatcgcggg ccacgaccgc caccagctcg tccgccccgt
     3301 cgccgtcgag gtcgaacgat tgcgcctcgg tcgcgatctc ggcgagctgg accgcggctg
     3361 gctcctcggg cagcgggatc ccctcgatcg ggtccacggc agccacgggg ccgaccgcgc
     3421 tcaggacaag cgtggtcgcg acgactgcgc caacgaggac acggcggaag cgagccacgg
     3481 gtcgatcagc catgggcggc acgtatcggc tcgagggtgg ccgcgtcctc gagcgcggag
     3541 atatccccgg ggtccatgcc caggtacgcc gcgcgggcca cgcgacggag gatctttccg
     3601 ttgcgagtct tcggaaggcc ggggacgaga tggacggccg ccggcttgaa cgacttgccc
     3661 aggccatcca ccacggtctg cccaatcgcg gtggccaggg cgtcggacgc ctcggcgctg
     3721 ctgcgaacga ccgcgaagac gacgatcgac tcccccttca cctcgtccgg caccccgaca
     3781 gccagcgcct cgacgacgag cgggtgggcc accgcggccg actccacctc cgccggaccg
     3841 atccgcttgc cggcgacctt gatcgtgtcg tccgagcggc cgctgatgta ccagaaacca
     3901 tcgggatcga tccgcgccca gtcgccgtgg acccacgtcc cgggcagccg ggaccagtac
     3961 gcctcgaggt agcggtcggg gtcacgccag aagccctggg tcatccccgg ccatggctgg
     4021 cggatggcca gctcccccac ctcgccgcgg accgggagtc cgtcggcatc gaccacgtcg
     4081 gccgccatgc ccgggcacgg gccggcgaac gagcagggcg cgatgggcgt gatcgtggtg
     4141 cagcccagga tcccgccgct gatctcggtg ccgccggagt agttgatgat cggtacgcgc
     4201 tcctcgccga ccgcgcgaaa cagccaccac cacgggtcgg gattccacgg ctccccggtc
     4261 gagcccagga cacggaggga ggatcggtca tgagccgcca ccggttccga gccgtgggtc
     4321 atcagcgccc ggacggcggt ggggctgatc ccgagatggg tgatccgatg ccgttcgacg
     4381 aggtcccata gccggtccgg tccgggatgg tcgggcgtgc cctcgtagat gaccagcgtg
     4441 gcgcccagga gcagggcgcc ggccaccgcc cacgggccca tcatccagcc catgtccgtg
     4501 aaccagaaca gggtgtcgcc acgctggagg tcgaagcagt gggcgaggtc caccgcgctc
     4561 ttgagcggga atccgccgtg aacgtggacc gcgcccttgg gctttccggt cgtgccgctg
     4621 gtgtagatca gcatgtaggg gtcgttggca tcggtgcgga ccgagcgcag tggttcgtca
     4681 cccgcggaag ccagcgcctc gtcccaggcc acgtcccgac ccgccgtcca ctcggcccgc
     4741 ggtgccacgt caccgagccg gcggacgacg aggctgtgct gaacggtggg cgccccggcc
     4801 agcgccgcgt ccgccaccgc cttcaggtcc accgccttcc cccggcgcca gaatccgtcc
     4861 gcggtgacga gcagcctggc cccgcagtcc gccagacggc tggccaccgc gggcgccccg
     4921 tagccggaga agatcggcgt gtagaccgcg cccagcttcc caaccgccag gaccgcgacc
     4981 acggtctcgg gaatcatggg catgaagacg cccactcgat cgtcccggcc gacgcccagc
     5041 gccgccatcg cgcgagccgc gcggtcgacg tccgcccgca gcgccgcccg cgagtagcga
     5101 cggaccgatc cgtcctcgcc ctcccaggcc agggcggtct cgtcgggcgc gtcgcccgcc
     5161 ggctgatcca cgccggcgtc gacccagttg aacccggcgc ccacccacca gcgagcccat
     5221 tcggggccgt gtgacacgtc gagcgcggcc atcggcttgg ggtcgaatcg gacgccgatg
     5281 tcggccaccg cggccgacca gaaccatgcc gggtcggcct ccgcccgctc ctggagctcc
     5341 tccaggtcgg cgcagccgac atcggtcagg aaccggcgca gccggctccg atcgaggtat
     5401 tccgccgaag gtcgccaggc tgccgcggcc gaatcggtcc cgaagatcgg acctcgcgct
     5461 cgaaccgcca tgtgtctgtt cccgccctcg tcgatcgtcc gacctacatc gcccggatgc
     5521 cgagcaggtt gcgggcgatc accaggcgtt ggatctcgct cgtcccctcc ccgatctcgg
     5581 tcagcttcgc gtcgcgcagg tagcgctcga ccttgtactc gcggacgtag ccgtacccgc
     5641 catggacctg gatggcggca ttcgtggccc gcgccgccac ctcggaggca tacagcttgg
     5701 ccatcgccgc ctcggtcgtg aacggcttgc cctggtcctt gagccacgcc gcgcggtaga
     5761 tgagcaggcg ggcggcgtcg atttcggtcg ccatgtccgc gatcttgaac gcgacgccct
     5821 ggaacgagcc gatcgcccgc ccgaattgct ttcgctcctt cgcgtaggcc gatgcggcct
     5881 cgtaggccgc ctgggccagg cccagggcca tggccccgat cccgatcctg ccgccgtcca
     5941 gggtgcggag gaacagcttg tcgccctcgc cctcgtcgcc cagcaggttg tcggccggga
     6001 cccggcagtc cgcgaacagc agctccccgg tggccgacgc atggagcccc atcttctcct
     6061 cgagacggcc gaacgtgaag ccgggcgtgc ccttctccac gatgaaggcg gtgatctggg
     6121 aatcgtcctc cgcctcgccg gtccgggcca cgacgatgta gatgtcggcc acgccggcgt
     6181 tggtgatgaa ctgcttggcc ccgtcgatca cccagtcgcc gccgtcgcgc cgcgcccggg
     6241 tcttcgtccc ccgcgagtca ctacccgcgc ccggctcggt caggccgtag gcgccgagct
     6301 tctcgccgct ggcaagcggg cggaggaacc gctcctgctg gtccggggtt ccggccaggt
     6361 acagcggtcc cgctccgagc gatgtatggg ccgagacgat gattcccatc gagccggaga
     6421 cgcgggacag ctcttcgatc gtgatcgcat aggccaggtt gtccagcccg gtaccgccca
     6481 gctcaaccgg gtacgggacg ccgagcaggc ccagcgacgc cgcctcgcgg acgagctccg
     6541 ccgggaattc gccacggacc tccatctcat cggcctgcgg ggcgatgcgc cggtccgcga
     6601 agtcgcggac cgtgcggcgg atgtcctcgt gggcagacga gagcgcgaaa tccatcggtc
     6661 cgccgagtct atcagcggcc ttgtgcccgg acctatactg ccgccacgct gggagggtgc
     6721 gcggttgccc gacgttgacg tccgcctgga ccatgtaacc aagtcattcg gcgagatggt
     6781 cgccgtcga
//
LOCUS       DASYGU010000044         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_623555, whole genome shotgun sequence.
ACCESSION   DASYGU010000044 DASYGU010000000
VERSION     DASYGU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623555"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VGO32_01155"
     CDS             <1..479
                     /locus_tag="VGO32_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7199391.1"
                     /translation="IYTWRTMLGGNGFVDWLLAPFGLEGPGLNSLVGSWLVLTYLWLP
                     YMILPIQAGLERIPGSLLEASADLGARSGRTFRRVVIPLVLPSIAAGSIFTFSLTLGD
                     YITPDLISNTQFIGNVIYSNSSLGSLPLAAAYSMVPVAIMVVYLYVVRRLGAFEAL"
     gene            487..1287
                     /locus_tag="VGO32_01160"
     CDS             487..1287
                     /locus_tag="VGO32_01160"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199392.1"
                     /translation="MTESRLARFALRAMTALVLVFVYTPLVLVAIYAFNPSIVQTWPP
                     SGFTLQWFGEALTNPDVLAAFATSVVAATMATTIALVLGTLASLAVDRFAFFGRESVS
                     LLLLLPLALPGVVTGIALSATFQTVDVRFGLATVIVGHATFCVVIAYNNVLARLRRSP
                     RSFEEASADLGADTWQTFRRVTLPVMRTALLAGGLLAFALSFDEVIVTNFTAGPATET
                     IPLFIFRSIQRPNELPIVNVVALILVLLSVIPVYIAQRISGEGTGSVR"
     gene            complement(1284..1691)
                     /locus_tag="VGO32_01165"
     CDS             complement(1284..1691)
                     /locus_tag="VGO32_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7199393.1"
                     /translation="MAQPELPIYARHVFVCTAGEWCPAVDGDGLGVHAALKAEAEGAG
                     LGGDVRINRSGCFSQCGNGPMVVVYPDGVWYAAVTPADAREIVERHLVGGEPVERLRY
                     RPGSTGAHKLERDADSRPIGRTAPWPSDRGELG"
     gene            1849..2034
                     /locus_tag="VGO32_01170"
     CDS             1849..2034
                     /locus_tag="VGO32_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7199394.1"
                     /translation="MIQNEGGRLLTATEVAEMLHLHVNTVKRLGDRGELPFFRVVKRG
                     DRRFRYEDVVEFLHRAK"
     gene            2149..3303
                     /locus_tag="VGO32_01175"
     CDS             2149..3303
                     /locus_tag="VGO32_01175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV7199395.1"
                     /translation="MSDPLAVLTEARRLLDTGYPEQAIATVADLTQSHDPEVAGAAWM
                     VAGTAKYRIDDEAGALQAWMAAAQSEGAASWIGWRSVAEQQVRDGDLNAAIEAYKESD
                     RRAPPEERPAIASRLAWLAKETGHDATARREFNRSRAQYATYTPIVSWALIGANVGVF
                     AIDAFLGGLAGLGLMSGGGGPLMDWGYVSADAVAAGEWWRILTSAFLHFGILHLVLNM
                     WALYLFGPLLEQLYGHIEYLVIYLLCAAGGSVLTILVAPEQAAVGASGAIFGLLGLAF
                     AVSRRRHLALPHQTRAVLGQIGSLLVINLAFTFFVPGISITGHLGGLAVGLVLGWLLP
                     PSPAFTLAGQWQAASGAVIEGTSMYLRVVVYLGMAALLAVGTLFVMGNFV"
     gene            3323..4093
                     /locus_tag="VGO32_01180"
     CDS             3323..4093
                     /locus_tag="VGO32_01180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7199396.1"
                     /translation="MCHDTHARPPLPPIRGGAVDAREVELTSADGTRFAAHSAVAAHP
                     GGPGIVILPDVRGLHPFYEDLANRLAEAGVHALALDLYARTAGPGRRGADFEYMPHVD
                     RTQHPSIAADVHAGVESIRTPAFGGAERVFTMGFCFGGRASFAQAAGGHGLAGVIGFY
                     GSVAGPGRAGIPAPIDLVSSYECPVLGLFGGADEGIPAESIAAFDEALTAAGIEHRLI
                     SYAGAPHSFFDRSAAEHADAATDAWRQVLEFVGVPLPD"
BASE COUNT          561 a         1517 c         1366 g          731 t
ORIGIN      
        1 agatctacac ctggcgcacg atgctcggcg gcaacgggtt cgtggactgg ctcctcgccc
       61 cgttcgggct cgagggaccc ggcctgaact cgctggtggg cagctggctg gtgctgacct
      121 acctctggct gccgtacatg atcctgccca tccaggccgg gttggagcgg atccccggat
      181 ccctgctcga ggcatccgcc gatctcggtg cccgctccgg ccggacgttc cggcgggtgg
      241 tgatcccgct cgtcctgccc tcgatcgccg cgggctcgat cttcaccttt tcactgaccc
      301 tgggtgacta catcaccccc gacctcatct cgaacacgca gttcatcggc aacgtcatct
      361 acagcaattc gagccttggc agcttgccac tggccgccgc ctattccatg gtcccggtcg
      421 cgatcatggt cgtctacctg tacgtcgtac gacggcttgg ggccttcgag gccctgtgag
      481 cgaccgatga ccgaatcgcg cctcgcccgc ttcgcactcc gcgccatgac cgcgctcgtc
      541 ctcgtcttcg tgtacacgcc cttggtcctg gtcgcgatct acgccttcaa cccgtccatc
      601 gtccagacct ggccgccttc ggggttcacc ctgcagtggt tcggcgaagc gctgaccaac
      661 cccgatgtcc tcgccgcctt cgccacctcc gtggtggccg ccacgatggc caccacgatc
      721 gcgctggtcc tgggaacgct cgcctcactg gccgtcgatc gtttcgcctt cttcggccgc
      781 gagtcggtct cattactgct cctcctgccc cttgccctgc ccggggtggt gaccggcatc
      841 gcgctgtcgg ccaccttcca aacagtcgac gtccggtttg ggctggcgac ggtgatcgtc
      901 ggccacgcga cgttctgcgt ggtcatcgcc tacaacaatg tccttgcccg gctccggcga
      961 tccccgcggt cgttcgagga ggcctccgcg gacctcgggg cagacacgtg gcagacgttc
     1021 cgacgggtga ccttgccggt catgcgcacg gcgctgctgg cgggcggcct gctggcgttc
     1081 gccctctcgt ttgacgaagt catcgtgacc aacttcacgg ccggccccgc gactgaaacg
     1141 atcccgctgt tcatcttccg cagcatccag cgtccgaatg agctgccgat cgtgaacgtt
     1201 gtggccctca tcctggtcct gctttccgtg atcccggtct acatcgccca gcggatcagc
     1261 ggtgagggca ccgggtccgt ccgctagccg agctcgcctc ggtcgctggg ccacggcgcg
     1321 gtccgaccga tgggccggct atcggcgtcc cgttcgagct tgtgggcccc ggtcgagccg
     1381 ggccgatagc ggaggcgttc caccggctcc ccgccgacca ggtgccgctc gacgatctcc
     1441 cgggcgtccg ccggggtgac ggcggcatac cagacgccat cgggatagac gacgaccatc
     1501 ggtccgttgc cgcactggga gaagcatccg gagcggttta tgcggacatc gccgcccagg
     1561 ccggccccct cggcctccgc cttgagcgcg gcatggacgc cgagcccgtc gccgtccacg
     1621 gcggggcacc actcgcccgc ggtgcacacg aacacgtggc gagcgtagat cgggagctca
     1681 ggttgggcca tcgggtctcc gaatgtactc ccggatttca ccaaacgtac cgggggtccg
     1741 cagaacaccg gaaccctact atccgctcac gagccgcgcc gaccagcacc ctccgagccg
     1801 gcgcggctca tacctcacca aagtggcgtg ggagaacagc acaccaagat gattcagaac
     1861 gaaggcggca gactgctgac ggcgaccgaa gtggccgaaa tgctgcatct gcacgtcaac
     1921 accgtgaagc ggctgggcga ccgtggcgag ctgccgttct tccgtgtcgt gaagcggggc
     1981 gaccgtcgct tccgatacga agacgtcgtg gaattcctcc accgggccaa gtagcccgat
     2041 tcctcaccgc ccggggcgac gggcgggctg gctcccctcc aggggggcgc agacgaccgc
     2101 cggacactgt ccggcggtcg ttcgctttgc gcggcatact acgccggcat gtccgacccc
     2161 ctcgccgtcc tcaccgaggc gcgccggctc ctcgacacgg ggtatccgga gcaggcgatt
     2221 gccacggttg cggatctcac ccagtcccat gatcccgagg ttgccggcgc cgcctggatg
     2281 gtggctggga ccgccaagta tcgaatcgac gacgaggccg gcgcgctcca ggcctggatg
     2341 gctgccgccc agtccgaggg ggctgcctcc tggatcggct ggcggtcggt cgccgagcag
     2401 caggttcgcg acggtgacct gaacgcggcc atcgaggcgt acaaggagtc cgatcgccgg
     2461 gcgccacccg aggagcggcc cgcgatcgcc agccggctcg cctggctggc caaggagacc
     2521 ggccacgacg ccactgcccg ccgcgagttc aaccgctcac gcgcgcagta cgcgacctac
     2581 accccgatcg tgtcgtgggc cctcatcggc gccaacgtgg gcgtcttcgc gatcgatgcg
     2641 tttctgggtg gcctggccgg gctggggctg atgtccggcg gcggcggccc gctcatggac
     2701 tggggctacg tcagcgcgga cgcggtcgcg gctggggagt ggtggcggat cctgaccagc
     2761 gccttcctcc acttcggcat cctgcacctg gtcctcaaca tgtgggcgct atacctcttc
     2821 ggcccgctcc tcgagcagct gtacgggcac atcgagtacc tggtcatcta tctcctctgc
     2881 gcggccgggg ggtcggtcct gaccatcctc gtcgccccgg agcaggcggc cgtgggcgcg
     2941 agcggcgcga tcttcggcct cctcggtctc gcctttgccg tgtcccggcg ccggcacctg
     3001 gcgcttcccc accagacccg cgccgtgctg ggccagatag gctcgctgct cgtgatcaat
     3061 ctcgcattca ccttcttcgt gccggggatc agcatcaccg gccaccttgg cggcctggcc
     3121 gttggcctcg tcctcggctg gctgctccca ccgtcgccgg cgttcaccct cgccggacag
     3181 tggcaagccg ccagcggggc ggtgatcgag ggcacgtcca tgtacctgcg ggtggtcgtg
     3241 tacctgggga tggcggccct gctcgcggtt ggcaccctgt tcgtgatggg caacttcgtc
     3301 tgattccacg gagcgccgcc tgatgtgcca cgacacccac gcccgcccgc cactgccgcc
     3361 catccggggc ggggcggttg acgcccgcga ggtggagctg acctcggccg atggcacacg
     3421 gttcgccgcc cattccgccg tggccgcgca tcccggggga cccgggatcg tgatcctgcc
     3481 cgacgttcgc ggcctccatc cgttctacga ggatctcgcc aaccgactgg cggaggccgg
     3541 cgtccatgcg ctcgccctgg atctgtacgc gcgcaccgcg ggtccgggcc ggcggggagc
     3601 tgacttcgag tacatgcccc acgttgaccg gacccagcac ccctccatcg cggctgacgt
     3661 ccatgcgggc gtggagtcga tccggacgcc agcgttcggg ggcgccgagc gggtcttcac
     3721 gatgggcttc tgcttcggcg gccgggcgtc gttcgcccag gcagcgggcg gtcacggact
     3781 cgccggcgtg atcgggttct acggctcggt ggccggtccc ggacgggccg gcattccggc
     3841 tcccatcgac ctcgtctcgt catacgagtg tcctgtcctg gggctgttcg gcggagcgga
     3901 cgagggcatc ccggccgaga gcatcgccgc cttcgacgag gccctgaccg cggccgggat
     3961 cgagcatcga ctgatcagct atgccggcgc cccccactcg ttcttcgacc gctcggcggc
     4021 cgagcatgcc gacgccgcca ccgacgcgtg gcgccaggtg ctcgagttcg tcggggttcc
     4081 cctgcccgac tgaccgccgg atccggcgga cgcggcggct agaatcgcgg accgatggtc
     4141 gacttctccc tgaccgatga gcagcgcgag gtgca
//
LOCUS       DASYGU010000045         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_315348, whole genome shotgun sequence.
ACCESSION   DASYGU010000045 DASYGU010000000
VERSION     DASYGU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315348"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGO32_01185"
     CDS             <1..515
                     /locus_tag="VGO32_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HEV7199397.1"
                     /translation="APTASGGHFIGHEVAGAQLVEHRLEALALSRADIERATHLVRHH
                     MILYGPEWTDAAVRRFIGRVGPGQPMADLFTLRRADTAASAGPGARDAAAAELEARVA
                     ALQGSAVLTVGELAVNGSDLMGELNLAPGPEVGRLLDVLLQAVLDDPSRNEPGALLTL
                     AREAATETNA"
     gene            691..1809
                     /gene="galE"
                     /locus_tag="VGO32_01190"
     CDS             691..1809
                     /gene="galE"
                     /locus_tag="VGO32_01190"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV7199398.1"
                     /translation="MRILVVGGAGYVGSTSVETFVEAGHDVTVYDNLKTGHRDAVIRG
                     ARLIVGSLSDQNRLERVLREHRIQAVLHCAARSLVSESMEHPELYYRENVSNGVKLLE
                     ALRERRVPRIVFSSTAAVYGEPKRVPIAEADRTEPINPYGETKLAFEGAMRWYAACHG
                     FRAISLRYFNVAGATERNGEDHDPETHLIPIVLKVAAGELPDVRIFGQDYNTPDGTCI
                     RDYIHVRDLASAHLLALEATGEAEPSFEVYNLGSAAGFSVREVIEAARKVTGRAIPAR
                     VVKRRVGDPPSLVASSRRARRDLGWQPREASLEQMLADAWAWRLSHPTGYAKRKPRTP
                     RHGRSGGPDGRTDGDGDGVGPADEGGVRPERVAVPVTG"
     gene            complement(1806..2243)
                     /locus_tag="VGO32_01195"
     CDS             complement(1806..2243)
                     /locus_tag="VGO32_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV7199399.1"
                     /translation="MTAATARRLRRAGQRVTTQRLAVAAALAGSPRQVTAQELWERMR
                     PRHRRLGRATVFRTLEALSEAGLARRLEQDGHVYAYVACRPAHHHHLACRVCGTVEEV
                     GEELVADISRRARRELGFEIEDARLDFYGVCRRCAAGGAGGAA"
     gene            complement(2240..2986)
                     /locus_tag="VGO32_01200"
     CDS             complement(2240..2986)
                     /locus_tag="VGO32_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEV7199400.1"
                     /translation="MPPLLVALSATAVLSALTGGVLALRLVRQVGYVIAIGAGIRIGA
                     AFFDLLPESVELMGDRVGLAMAFAALGFVAFYVIEKVTVLHLGHETATELDHDEVAHQ
                     HIGLIGAVAMSIHSLLDGVAMAAAFQASPELGFVVGLVVVIHRFGDGIGIVSLLLASR
                     VPRPTAMRWVFLVSLAPVLGAAVTAFFVIPDELLGALLATFAGFFLYVGAAELLPEAH
                     RNEGSRWVIAATLTGVALIAFMSFGLEPQL"
     gene            3163..>3512
                     /locus_tag="VGO32_01205"
     CDS             3163..>3512
                     /locus_tag="VGO32_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7199401.1"
                     /translation="MPVPVDTVDESDSALTDAVMAAAAGYRFGDIEVPATIARALEQM
                     GYVTPTEVQARAIPPLRAGGDFIGQAQTGTGKTAAFGIPIVEQVDPSDKSVQALVLTP
                     TRELCQQVTDEIGR"
BASE COUNT          557 a         1287 c         1200 g          468 t
ORIGIN      
        1 aggccccgac cgcgagcggc ggccatttca tcggtcacga ggtcgcgggc gcccagctgg
       61 tcgagcaccg actcgaggcg ctggccctgt cccgcgccga catcgagcgg gcaacccacc
      121 tggtccgcca ccacatgatc ctgtatggcc ccgaatggac cgatgctgcc gtgcgccgct
      181 tcatcgggcg tgtcggtccc gggcagccca tggccgacct gttcaccctg cgtcgggcgg
      241 acaccgcggc gtccgccggc cccggggcgc gggacgcggc ggcggccgag ctcgaggctc
      301 gggtggccgc gctccaggga tcggccgtgc tcaccgtcgg ggagctggcg gtcaacggct
      361 cagacctcat gggtgagctc aacctcgctc cgggacccga ggtcggccgc ctcctcgacg
      421 tcctccttca ggccgtgctc gacgatccgt ctcgcaacga gcccggcgcg ctgctcacgc
      481 tggctcgcga agcggcgacc gaaacgaacg cgtgaaatcg gtcggccggt gcgccgaggc
      541 accgccatcc gaagacggaa cacccggagc gtgggaaggc cactgctaca atcggcggcc
      601 ggacccatcg gaggagccct tccccgaatg agcgtcagtg cccgacgggc ttgcccgccc
      661 gtccatcggt cagtgttcta ggttgcccgc gtgcggattc ttgtcgtcgg tggagcaggc
      721 tacgttggct cgacgagtgt cgagaccttc gttgaggctg gccacgacgt caccgtctac
      781 gacaacctga agaccggcca ccgcgacgcg gtcatccgtg gcgcccggct catcgtcggc
      841 agcctgagcg accagaaccg cctcgagcgc gtccttcgcg agcatcggat ccaggccgtc
      901 ctgcactgcg ccgcgcgttc gctggtcagc gagtcgatgg agcatccgga gctgtactac
      961 cgcgagaacg tctcgaacgg agtcaagctg ctggaggcgc tccgcgagcg acgggtgccc
     1021 cggatcgtgt tctcctccac ggccgcggtg tacggcgagc ccaagcgggt tccgatcgcg
     1081 gaagcggacc ggaccgagcc gatcaacccg tatggcgaga cgaagctcgc gttcgagggc
     1141 gccatgcgct ggtacgcggc atgccacggg ttccgtgcca tcagcctccg gtacttcaac
     1201 gtggcgggcg cgaccgaacg caacggcgag gaccatgacc cggagacgca cctcatcccg
     1261 atcgtcctca aggtggcggc tggagagctg cccgacgtcc ggatcttcgg ccaggactac
     1321 aacaccccgg acgggacctg catccgcgac tacatccacg tccgcgacct ggcctcggcc
     1381 cacctcctcg ccctcgaggc gaccggcgag gcggagccgt cgttcgaggt ctacaacctc
     1441 ggctccgcgg cggggttctc cgtccgcgag gtcatcgagg ccgcccgcaa ggtcaccggc
     1501 cgtgccatcc cggcccgggt cgtgaagcgc cgtgtgggcg accccccgag cctggtggcc
     1561 tcgtcccggc gggcccgacg tgatctcggc tggcagccgc gcgaggcatc actcgagcag
     1621 atgctcgccg atgcctgggc gtggaggctc tcccatccca ccggctacgc gaagcggaag
     1681 ccgcgaacgc cgcgccacgg ccgatcgggg ggccccgatg gacggaccga cggcgacggg
     1741 gacggcgtgg gcccggccga cgagggcggt gtccgacccg agcgggtcgc cgtgccggtg
     1801 accggctagg ccgccccgcc cgccccgccg gccgcgcatc gtcggcagac gccgtagaag
     1861 tccagccgcg catcctcgat ctcgaatccg agctcgcgcc gcgcgcgccg gctgatgtcg
     1921 gccaccagct cttcgccgac ctcctccacc gtcccgcaca cccggcaggc gaggtggtgg
     1981 tgatgggccg gtcggcaggc gacataggca tacacatgac cgtcctgctc gaggcggcgc
     2041 gcgagaccgg cctcgctgag ggcttcgagt gtgcggaaga ccgtcgcccg acccagccgt
     2101 cggtggcgcg gccgcatccg ctcccatagc tcctgggcgg tgacctgccg cggcgagccg
     2161 gccagcgcgg cggccaccgc cagccgttgg gtcgtgaccc gctgaccggc tcgacgcagc
     2221 cgccgggcgg tggccgcggt cacagttgcg gctccagccc aaagctcatg aacgcgatca
     2281 gcgcgacgcc ggtgagggtc gcggcgatga cccaccgtga gccctcgttg cgatgagctt
     2341 cgggcaggag ctcggccgcg ccgacgtaca ggaagaagcc ggcgaacgtg gccagcagcg
     2401 cgccgaggag ctcgtccgga atcacgaaga aggccgtgac ggcggcaccg aggaccggag
     2461 ccaggctgac gaggaagacc cagcgcatgg cggtcggccg cgggacccgg ctggcgagca
     2521 gcaggctgac gatcccgatc ccgtcgccga agcggtggat aacaaccacc aggcccacca
     2581 cgaacccgag ttcggggctg gcctggaacg cggccgccat cgcgacgccg tcgagcaggc
     2641 tgtggatcga catcgccacc gcaccgataa ggccgatgtg ctggtgcgcg acctcgtcgt
     2701 ggtccagctc ggttgcggtc tcgtgtccga ggtggaggac ggtcaccttc tcgatcacgt
     2761 agaacgccac gaatcccagc gcggcgaagg ccatcgccaa cccgacccgg tctcccatca
     2821 gctccaccga ttcgggcagc aggtcgaaga aggcggcgcc gattcgaatg ccggccccga
     2881 tcgcgatgac gtacccgacc tgtcggacga ggcgcagggc caggaccccg ccggtcaacg
     2941 cggagaggac ggcggtggcc gatagggcaa ccaggagcgg gggcatacct gatacgcagt
     3001 atcaaacacg gcgagggcgc aggtcaagcg cagggggcca ccgtgctacg atcggcccac
     3061 ttcattcacc accaatcgga gccctgtttg ccccccaccc cgaccacgcc ccgccgccga
     3121 actcgcagcc gctcacacca atcgcccgcc cctgacggcc gcgtgcccgt ccccgtcgac
     3181 accgtcgacg agtccgattc cgccctgacc gatgccgtca tggccgccgc cgcgggctac
     3241 cgcttcggcg acatcgaggt gccggccacg attgcccggg ccctcgagca gatgggctac
     3301 gtgaccccca ccgaggtcca ggcccgcgcc atcccgccgt tgcgtgcggg cggcgacttc
     3361 atcggccagg cgcagaccgg cactggcaag accgcggcct ttggtattcc gatcgtcgaa
     3421 caggtggatc cgtcggacaa atcggtccag gccctggtcc tgacccccac ccgcgagctg
     3481 tgccagcagg tcaccgacga gatcgggcgc at
//
LOCUS       DASYGU010000046         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_366739, whole genome shotgun sequence.
ACCESSION   DASYGU010000046 DASYGU010000000
VERSION     DASYGU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366739"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..213)
                     /locus_tag="VGO32_01210"
     CDS             complement(61..213)
                     /locus_tag="VGO32_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199402.1"
                     /translation="MNIIKTIAIAIGAILAALIIVPLTVLAGLFVWLKLTEEVDDEPW
                     EIDDAI"
     gene            complement(210..797)
                     /locus_tag="VGO32_01215"
     CDS             complement(210..797)
                     /locus_tag="VGO32_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199403.1"
                     /translation="MRIYEGSPRQDWEEVLRAIGAWADREFLKELLFLELDGGFLVQG
                     ITSSMGGQWSESGTFAKQTHELTDDQVGDLIGAAQEQRGSAGVDHAEVGIANFYEQAL
                     RIIGKWLDSTQPRDVFLFEQGGSFVVRTFMVGAHGKVGHQLAEFTRDEIVAMIESAPL
                     DRGRGSAAAPAPAASAAPPPAAAAPAPAGESETET"
     gene            complement(824..1744)
                     /locus_tag="VGO32_01220"
     CDS             complement(824..1744)
                     /locus_tag="VGO32_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEV7199404.1"
                     /translation="MSAPWISLSDEVRDALEAGRPVVALESSLIAQGLPAPHNLETAR
                     AAEAAVRDQGAIPATSAFVNGRAIVGADAATLERLADPAERPLKAGSRDIGPVIASRA
                     LASTTVSAALRVAHVAGIRMMATGGIGGVHRDAARTFDISADIDELAATPVVVVCSGA
                     KAILDLPATMELLESRRVPVVGLGTDELPAFYSRSSGLRLAHVADGPAAAAGIVAAHM
                     AVPGAGGMLLVNPPPEAVAIPAEDVADWIAEAMAAADAEGVRGPAVTPFLLRHVAEAS
                     AGRALRANIGLIVDNARAAAEVAVSMRGVS"
     gene            complement(1744..2682)
                     /locus_tag="VGO32_01225"
     CDS             complement(1744..2682)
                     /locus_tag="VGO32_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEV7199405.1"
                     /translation="MTNRPTRVTVIGDSTLDITVLDAVPVPGRDHPAQIIAGPGGQGA
                     NVAVRLARQGVIVTLVTAVGTDPAGQVLSATLEAEGVRVVSAAAEVGRSGAVVALVDR
                     AGERAMLSDRLPLDSAVQDAPAVRAALTDADWIHLSGYPLADPQGGAALAKLAGSRPD
                     RQRCSIAGGSFHDDAALTARIRTARPDLVVFDRAEAATILGSAAPAGDRAAGALATAL
                     VAALGGVAIVTDGASGAAAAATEGAVEVRGRARSVLDATGAGDAFAASLIAALSGEGW
                     PPSLAVLQHALENAAARGADVAGAVGAQARLGSEAP"
     gene            complement(2679..3080)
                     /locus_tag="VGO32_01230"
     CDS             complement(2679..3080)
                     /locus_tag="VGO32_01230"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEV7199406.1"
                     /translation="MAEGRPTYTIDEANALLPQVRAVLLQLAVEKAKLETAVNALAAH
                     GSSETNGNAAHADAMARSEAEMTQIGGGMRALVEHLESLGIELRDLEQGLVDFPGERD
                     GRPVWLCWVLSEPAVAYWHPIDQGFVSRQPW"
     gene            3172..4233
                     /gene="speB"
                     /locus_tag="VGO32_01235"
     CDS             3172..4233
                     /gene="speB"
                     /locus_tag="VGO32_01235"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEV7199407.1"
                     /translation="MDDALAGRGKNDPAPPDDADLLHEWDDFNLPSYLGSTTFQKLPL
                     VVDADALAARRPDVVILGAPLDDGVSHRPGARFGPRAIRTASYHSGDIHSLQVGVDPF
                     DWLDVVDGGDAPVVPGRLERGHAVIRARVLEIARTGAIPIVLGGDHSITYPSASAVAE
                     AVAPRRLGIVHFDAHADTGDNTWGNLLSHGTPMRRLIQSGAVAGRNFVQVGLRGYWPP
                     AETLAWMREQEMRWHLMTEIESRGAEAVVHDAIAEALDGPDVVYLSVDIDVIDPGMAP
                     GTGTPEPGGMLTRELLRAIRQVVGAVDLVGMDVVEVAPAYDVSDVTAAAAHRCVLEAI
                     SALAGRRRDAREPARPTVR"
     gene            complement(4240..>4592)
                     /locus_tag="VGO32_01240"
     CDS             complement(4240..>4592)
                     /locus_tag="VGO32_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199408.1"
                     /translation="TPTPVPAPDGFIARAEACDEQPTDSTCDSSGATNDGHVWILVSF
                     RHGQATDVIGVSIQDESGSVVDQGSFPLNFCGASTDCAGYTYFEFSNLADGDYDVVAT
                     RNEDEVATTTFTVE"
BASE COUNT          648 a         1641 c         1642 g          661 t
ORIGIN      
        1 atgtggacga tcgcctgggg gctggccagc gggccggccg tctagggccg gagcgccggc
       61 ctagatcgcg tcgtcgatct cccagggctc gtcgtcgacc tcttcggtca gcttgagcca
      121 gacgaagagg ccggcgagga cagtcagcgg gacgatgatg agggcggcca ggatggcgcc
      181 gatggcgatg gcgatggtct tgatgatgtt catgtctcgg tctccgattc acctgccggt
      241 gcgggagccg cggcggcggg cggtggtgcg gcagaggcag ccggggctgg cgccgccgcg
      301 gatccccgtc cgcgatcaag aggcgccgac tcgatcatag cgacgatctc gtctcgggtg
      361 aattcggcca gctggtgacc caccttcccg tgagccccga ccatgaatgt tcggaccacg
      421 aaggacccgc cctgctcgaa caggaagacg tcacgcggct gggtgctgtc aagccatttg
      481 ccgatgatcc gcagggcctg ctcgtagaag ttggcgatgc ccacctcggc gtggtcgacc
      541 cccgctgacc cacgctgctc ctgcgcggca ccgatgaggt cgccgacctg gtcgtcggtc
      601 agctcgtggg tctgcttggc aaacgtgccg gactcggacc attgcccgcc catcgaggac
      661 gtgatgccct ggacgagaaa cccgccgtcg agctcgagga agagcagctc cttcaggaac
      721 tcgcgatccg cccacgctcc aatagcgcgc aggacctctt cccagtcctg gcgcgggctc
      781 ccctcgtaga tgcgcatcgc gtcctccggc tgatggcccg cggctaggat accccgcgca
      841 tcgacacggc cacctcggcc gccgcccgag cgttgtccac gatcagaccg atattggcgc
      901 gcagcgcgcg cccggcggat gcctcggcga cgtggcgcag caggaacggg gtgaccgccg
      961 ggccgcgcac gccctccgcg tcggcggcgg ccatggcctc cgcgatccag tcggcaacat
     1021 cctcggccgg gatggcgacc gcctcgggcg gcgggttgac gaggagcatt ccccccgccc
     1081 ccggcaccgc catgtgggct gccacgatcc cggccgccgc cgccggccca tccgccacgt
     1141 gggccagtcg caggccgctg gagcggctgt agaacgcggg cagctcatcg gtgccgaggc
     1201 ccacgaccgg cacgcggcga gactcgagca gctccatcgt cgccggcagg tcgaggatcg
     1261 ctttcgcgcc gctgcacacc acgacgaccg gggtggcggc cagctcgtcg atgtcagcgc
     1321 tgatgtcgaa cgtgcgggcc gcatcgcgat ggacgccgcc tatgcccccg gtggccatca
     1381 tccggatgcc ggccacgtga gcaacccgga gggcggcgct gaccgtggtc gaggccaggg
     1441 cgcgtgaggc gatgaccgga ccgatgtcgc gcgagccggc tttgagcggc cgctctgcgg
     1501 ggtctgcgag gcgctcgagc gtggccgcgt ccgccccgac gatcgcgcgg ccgttcacga
     1561 aggccgaggt ggcggggatc gcgccctggt cgcggaccgc ggcctcggcc gcccgagcgg
     1621 tctccaggtt gtgtggcgcg ggcagtccct gggcaatcag cgacgactcg agcgcgacca
     1681 ccggccgacc ggcctccaga gcgtcgcgca cctcgtcgct caacgagatc cagggggcgg
     1741 acatcacggc gcttccgagc cgaggcgggc ctgggccccg accgcgcccg cgacgtcggc
     1801 gccgcgcgcg gcggcgttct cgagggcgtg ttggaggacg gcgagcgatg gtggccagcc
     1861 ctcgccagac agcgcggcaa tgagcgaggc cgcgaacgca tcccccgcgc cggtcgcgtc
     1921 cagcaccgat cgggcccgcc cgcggacctc gaccgccccc tcggtcgcgg cggccgcggc
     1981 cccggacgcg ccgtcggtca cgatcgcgac cccaccgagg gcggccacca gcgccgtcgc
     2041 cagcgcgccg gccgctcgat cccctgccgg tgccgcggag ccgaggatcg tcgcggcctc
     2101 cgcccggtcg aacacgacga ggtcaggtcg ggcggtccgg atacgggccg taagagccgc
     2161 atcgtcgtgg aacgagccac ccgcgatgct gcatcgctgc cggtcaggtc gcgacccggc
     2221 caatttcgcg agcgcggcac caccctgcgg gtcggcgaga ggatagcccg acaggtggat
     2281 ccagtccgca tccgtcaggg ccgcacggac cgctggcgca tcctggacgg ccgagtcgag
     2341 cggtaggcgg tcggagagca tcgcgcgctc gccggcacga tccaccaggg cgaccaccgc
     2401 accgctgcgc ccaacctcgg cggccgcgct cacgaccctg accccttccg cctcgagcgt
     2461 ggcggacagc acctgcccgg ccggatcggt cccgaccgcc gtgaccaggg tgacgatcac
     2521 gccctgccgg gccagtcgaa cggcgacgtt cgcgccttgc cccccgggcc cggcaatgat
     2581 ctgcgccggg tggtctcgtc cgggtacggg gaccgcgtcg agcacggtga tgtcgagggt
     2641 gctgtcgccg atgaccgtca cccgcgtcgg ccggttggtc accacggctg gcggctgacg
     2701 aatccctggt cgatcggatg ccagtaggcg acggccggct cggacaggac ccagcacagc
     2761 catacggggc gaccgtctcg ctcaccggga aagtcgacaa gcccctgctc gagatcgcgc
     2821 agctcgatgc cgagcgactc gaggtgctcg accagcgccc gcatgccgcc gccgatctgg
     2881 gtcatctcgg cctctgaccg ggccatggca tcggcgtggg ccgcattgcc gttggtctcg
     2941 ctgctcccgt gtgcggccag cgcattgacc gcggtctcga gctttgcctt ctccaccgcg
     3001 agctggagga gcaccgcgcg aacctgcggc aggagcgcat tcgcctcgtc gatggtgtac
     3061 gtcgggcggc cttcggccat cgccggatgg tacccccctc tgctacgatc gaagcgcccg
     3121 gcgggccagc tccgcgccgc gacggcgccc ccggtgcgag gcccagatgc catggacgat
     3181 gctctggccg gtcgcggcaa gaacgacccg gctccgcctg acgacgcgga cctcctgcac
     3241 gagtgggacg acttcaacct gccgtcctac ctgggcagca cgaccttcca gaagctgccg
     3301 ctggtggttg acgccgacgc gctcgcggcg cgacgacctg atgtcgtgat cctgggcgcc
     3361 cccctggacg atggcgtgag ccaccggccg ggcgcacgat tcgggccgcg cgcgatccgg
     3421 accgcctcgt accactcggg cgacatccac tcgctccagg tgggcgtcga cccgttcgac
     3481 tggctggacg tagtggacgg tggcgacgcc ccggtggtcc cgggccgcct cgagcgtggc
     3541 catgccgtga tccgggcgcg ggttctggag atcgcgcgaa ccggcgcgat cccgatcgtg
     3601 ctgggcggtg accactcgat cacctacccg tcggccagcg cggtggcgga ggcggtggct
     3661 ccccggcgac tggggatcgt gcatttcgac gcccacgccg acaccggcga caacacgtgg
     3721 ggaaaccttc tgtcccatgg gacgccgatg cgacggctca tccagtcggg tgccgtcgcc
     3781 ggccgcaact tcgtccaggt cgggctccgc ggctattggc cgccggctga gacgctggcc
     3841 tggatgcgcg agcaggagat gcgctggcat ctcatgaccg agatcgagtc acgcggcgcc
     3901 gaggccgtgg tccacgacgc gatcgcggaa gccctggacg gtcctgatgt cgtgtacctc
     3961 tcggtggata tcgacgtcat cgatccgggc atggcgccgg ggaccgggac cccggagccg
     4021 ggcgggatgc tgacccgtga gctgctccgc gcgatccggc aggtcgtcgg cgccgtggac
     4081 ctggtgggga tggacgtggt cgaggtggct cccgcctacg acgtgtccga cgtcaccgcc
     4141 gcagccgccc atcggtgcgt gctcgaggcg atcagtgcgc tcgccggaag gcggcgcgat
     4201 gcccgggagc cggctcgccc gaccgtccgc tgaaggcggc tactcgaccg tgaatgtggt
     4261 cgtcgcgacc tcgtcctcgt tgcgggtggc caccacgtcg tagtcgccgt cggccaggtt
     4321 gctgaattcg aagtatgtgt agccggcgca gtcggtgctg gccccgcaaa agttcagggg
     4381 gaaactcccc tgatcgacga ctgagcccga ctcgtcctgg atgctgaccc cgatcacgtc
     4441 cgttgcctgg ccgtggcgga aactcaccag gatccagaca tggccgtcat tcgtggcacc
     4501 gctgctgtca caggtcgagt cagtgggctg ctcgtcgcag gcctcggcac gggcgatgaa
     4561 cccgtccggg gcggggacgg gggtcggggt cg
//
LOCUS       DASYGU010000047         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_469907, whole genome shotgun sequence.
ACCESSION   DASYGU010000047 DASYGU010000000
VERSION     DASYGU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469907"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VGO32_01245"
     CDS             complement(<1..710)
                     /locus_tag="VGO32_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7199409.1"
                     /translation="MTDITAPTPAVGDGGPDMVQLLTPEGQRVANAAAADYAHYVEDL
                     TDEDMRGLYRDLVLIRRVDTEATALQRQGELGIWASLLGQEAAQVGSGRAMKKQDYAF
                     PTYREHGVAWCRGVDPLNLLGLFRGVNHGGWDPNEKNFHLYTIVIGAQTLHATGYAMG
                     IQRDGAVGTGDPDRDAAVIAYFGDGATAQGDVSESFVFAAVNNAPIVFFCQNNQWAIS
                     EPNDRQTRIPLYQRARGFG"
     gene            1104..>1719
                     /locus_tag="VGO32_01250"
     CDS             1104..>1719
                     /locus_tag="VGO32_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEV7199410.1"
                     /translation="MDGWQALADGHGIVSASAAQSAGLTTRELRLLVRSEALMRLDRG
                     WYALADVLGGPADSPWDRRRRLHALRARAVIRAYEGRVAASHHTALVLCDLPTFAADL
                     RQVHVTRVDDGQFRRRPGLTVHEQTHGATPSDGRVGVATAIMGTGRINGQMAGLIAAD
                     AALHRQLVTHDDLVAAARSLVGPASALARRVALLADGRAESPGET"
BASE COUNT          230 a          586 c          642 g          261 t
ORIGIN      
        1 ccgaacccgc gggcgcgctg gtagagcggg atgcgggtct gccggtcgtt cggctcggag
       61 atggcccact ggttgttctg gcagaagaag acgatcgggg cgttgttgac ggcggcgaag
      121 acgaacgact cgctgacgtc gccctgggcg gtcgcgccgt cgccgaagta ggcgatgacg
      181 gcggcgtcgc ggtcggggtc cccagtgccg accgctccgt cgcgctggat ccccatggca
      241 tacccggtcg cgtggagcgt ctgcgcgccg atgacgatcg tgtagaggtg gaagttcttc
      301 tcgttggggt cccagccgcc gtggttgacg ccgcggaaga ggccgagcag gttgagcggg
      361 tcgacgccgc ggcaccaggc gacgccgtgc tcgcggtagg tcgggaatgc gtagtcctgc
      421 ttcttcatcg cgcggccgga gccgacctgg gcggcctcct gaccgagcag tgacgcccag
      481 atgccgagct cgccctggcg ctgcaacgcg gtggcctcgg tgtcgacgcg ccggatgagg
      541 acgaggtcgc ggtagaggcc gcgcatgtcc tcgtcggtga ggtcctcgac gtagtgggcg
      601 tagtccgcgg cggccgcgtt ggcgacgcgc tggccctcgg gggtgagcag ctgaaccatg
      661 tccgggccgc cgtcgccgac ggccggcgtc ggggcggtga tgtccgtcat ctcgctcacg
      721 ggagctcctt ctgtgggcgg ccgatccagg gcgcccgcgt tccaggccgc ggctccaccc
      781 ggggtcaccg gagggggcca cggcaactcg atgcgagagc ggtctgggta cgcccgcact
      841 cgccgatgag ccgttcgagc caaatctatc cgggcaccct ggaaatctca caatgcggac
      901 gccccggccc acgcccacgg cctgtcaggc ccttttggtc acgttgtgga ctgggtggtc
      961 agggtgcagc ccgatgggtg cgtcgatacc ccgaccagaa cgcctgatag ccgatgtcca
     1021 cagcccgtcg ccactccgtc acaccgtcca cagcgccacg ccgtggtctg gagcggcggt
     1081 tcggcctccg ccaccgttga cccatggacg ggtggcaggc actggccgat gggcacggca
     1141 tcgtgagtgc ctcggcggcg cagtcggccg ggctgaccac gcgagagctc cgcctcctcg
     1201 tgcgctcgga ggctctgatg cggctcgacc ggggctggta cgcgctggcc gatgtgctcg
     1261 gcgggccggc ggactcgccg tgggaccggc ggcgccggct tcacgcactc agggcgcgcg
     1321 cggtcattcg ggcctacgag ggacgggtcg ccgccagcca ccacacggcc ctcgtgctgt
     1381 gtgacctgcc cacctttgcc gccgacctcc ggcaggtgca cgtgacgcgg gtcgacgatg
     1441 gccagttccg tcgccgaccc gggctgaccg tccatgagca gacccacggt gcgaccccct
     1501 cggacggcag ggtcggtgtg gccacggcga tcatgggcac cggccggatc aacgggcaga
     1561 tggcagggct catcgcggcc gacgcggcgc tgcatcgaca gctggtgacg cacgatgacc
     1621 tcgtggcggc ggcgcgaagc cttgtcggac ccgcatcggc cctggcgcgc cgcgtcgcgc
     1681 tcctcgcgga tgggcgcgcg gagtcaccag gagagaccc
//
LOCUS       DASYGU010000048         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1241836, whole genome shotgun sequence.
ACCESSION   DASYGU010000048 DASYGU010000000
VERSION     DASYGU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241836"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1638)
                     /locus_tag="VGO32_01255"
     CDS             complement(106..1638)
                     /locus_tag="VGO32_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7199411.1"
                     /translation="MIAAHLPRLGTAMLAGFAVLMVAMSWWQVVTASALEARPDNPAL
                     IAAHRSQPRGTIFDREGVVLASTQVIDDLARRTYLDAASTHLLGYTSLRYGTAGVERA
                     WDELLIGLRDPNPLHDVVNDVLQRPPEAQDLVLTVDHRLQVLASDLLSGSVGAVVAID
                     PATGAVLALASSPTFDATAISGDPATAEEPWLALQQDPNLPLVNRALNGVYVPGSVMK
                     VLTAGSALDAGVITPDTTFPTQPQEEVDGLVVNGFTIVEHDLAGVTPALWDLSEAMQI
                     SSNIYFAHVGLALGQEPYLAGAEAFGFCDDLRVGPAGHALSVSASSVTALADPGCEPL
                     VDDAELASAAFGQGRVVATPVQMALVAATIANGGTMLDPFVVSEVHVPDPDGGAGTTV
                     AGFDTPGRRQVISRVAADQVRSAMVDAVHGPLGAPYAGAANVANYGIAGVETAGKTGT
                     AERGEGLPPHSWFIGFAAAQPGATPEIAVAVIIEGGGPGSASAAPIGGRVMAEWLRLS
                     GF"
     gene            complement(1635..2963)
                     /locus_tag="VGO32_01260"
     CDS             complement(1635..2963)
                     /locus_tag="VGO32_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV7199412.1"
                     /translation="MYRLAARRTELSLLAPILLLVPLGVLVTNIAQSDTAEIGPMAVA
                     IAFIGLVAVAHFALAWYGHRGDELLLPAVAGMGALGVIMLNRLPQDLAGNAALNLGMV
                     GTQLVWFGVGLGAMVLIAIGLRDDGLLRHYKYSWGLFAALLLVITFLFGTEINQARLW
                     IVVGPFGFQPSEAIKVMLVVFLAGYLAENRAVLAGASLRLGPLTLPPLPYLAPMLIVF
                     AAVMLVVVVSRDLGTALMFFGIFLTMLFVATGRRAYVLFGMILFVLGATVAYLLFPHV
                     QVRIDAWLDPWADPSGSGYQAIQALYAFARGGLVGEGLGQGLPTINGVLPIPAVHTDM
                     IFAAIGEELGLVGGVALLGLVAVVMARGLRIAILARDDFSTMLAAGLTAGLVLQSLII
                     IGGNLKVVPLTGITLPFVSYGGSSLLASFVVVGLLLSISHRSMVAGDRDR"
     gene            complement(2966..3409)
                     /locus_tag="VGO32_01265"
     CDS             complement(2966..3409)
                     /locus_tag="VGO32_01265"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7199413.1"
                     /translation="MTNDILRLILLALQLGFLALLVLIVLRFARSLRADLRQAAAIEA
                     ASRTGIGRLVVLESPGGEPAVDRVLHIGPITTLGRDVNSTIYLDDEFASGTHAVLTFR
                     GRSWYAEDRGSTNGTWVNGHRIERPVALTFGDELTIGRVRFRLER"
     gene            complement(3423..>4113)
                     /locus_tag="VGO32_01270"
     CDS             complement(3423..>4113)
                     /locus_tag="VGO32_01270"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEV7199414.1"
                     /translation="RMGAAPQPVTLAKRIARAMDTNKRFGEDGVIVPNRYVVHLSPTD
                     YASFESYHGSLEDDLAHEVLTRSRREGYRLMARPRVILQADGLVPRGDVRVAAAVADN
                     DAAPIVERQMADQTSVLARPGHAVPDPNTASRAYLVVQTDGGTAARFDLGGALIAIGR
                     ATDNDVILDDPQVSRHHCQLKVQHGAYGFVDLQSRNGSTVNGHAVAEIALADGDRIRI
                     GNTNVDFRLHG"
BASE COUNT          679 a         1379 c         1493 g          562 t
ORIGIN      
        1 gttcaagggc gggctcctga tgatgggggt tgcgtcaccg gacaataccc cagccggtgg
       61 ccgagcggtt cacgaccggt aggacgcgga cggaccgggt gcggttcaga atccgctcag
      121 gcgaagccac tcggccatca cccggccgcc gatcggggcc gcggacgcgg atcccgggcc
      181 gccgccctcg atgatcaccg caacggcgat ctcgggggtc gccccgggct gcgcagcggc
      241 aaagccgatg aaccacgaat gcggaggaag gccttctccc cgctcggcgg taccggtctt
      301 gcccgcggtc tccacgcccg cgatcccgta gttcgcgacg ttcgccgcgc ccgcatacgg
      361 cgcgcccagc ggcccgtgga ccgcgtcgac catggccgac ctgacctggt ccgcagcgac
      421 ccggctgatg acctgccgcc gccccggggt gtcgaatccg gccaccgtgg ttcccgcacc
      481 accgtccgga tcgggcacgt ggacctcgct gaccacgaat gggtcgagca tggtgccgcc
      541 gttcgcgatg gtggccgcga ccagcgccat ctggaccggc gtcgcgacca cccggccctg
      601 gccgaaggcg gccgaggcca gctccgcgtc gtccaccaac ggctcgcagc cgggatcggc
      661 gagggcggtc accgacgagg ccgatacgga aagggcgtgg ccggcgggtc caacccgcag
      721 gtcgtcgcag aacccaaagg cctcggcacc ggccaggtac ggctcctgcc cgagcgcgag
      781 tccgacgtgg gcgaagtaga tgttgctcga gatctgcatc gcctcggaca ggtcccacag
      841 ggccggtgtc acgccggcga gatcatgctc gacgatcgtg aagccgttca ccaccaggcc
      901 gtccacctct tcctggggct gggtcgggaa cgtcgtgtcg ggggtgatca ccccggcgtc
      961 cagggcggag ccggcggtca ggaccttcat caccgagccg gggacgtaga ccccgttcag
     1021 ggcacggttg acgagcggca ggttggggtc ctgctgaagg gcgagccacg gttcctcggc
     1081 cgtcgccgga tcaccgctga tcgcggtcgc gtcgaacgtc gggctggagg ccagggccag
     1141 gaccgcaccg gttgccgggt cgatcgccac gaccgcgccc acgctgccac tgagcaggtc
     1201 gctcgccagc acctggagtc ggtggtcgac ggtgagcacg aggtcctggg cctcgggcgg
     1261 gcgctgcagg acgtcgttga ccacgtcatg gagggggttc ggatcgcgca gaccgatgag
     1321 cagctcgtcc caggcccgct cgactccggc cgtcccgtag cgcagcgacg tgtagcccag
     1381 caggtgggtg gaggccgcgt cgaggtatgt ccggcgcgcg agatcgtcga tcacctgggt
     1441 cgaggccagc accacgccct cccggtcgaa gatcgttccc cgaggctggc tgcggtgggc
     1501 ggcgatgagc gcggggttgt ccggtcgtgc ctcgagggcc gatgcggtga ccacctgcca
     1561 ccaggacatc gcgaccatca gcacggcgaa gccggcgagc atggcggttc cgagtcgggg
     1621 cagatgggcg gcgatcatcg gtcccgatct ccggccacca tcgaccgatg gctgatggac
     1681 aggagcaggc ccaccaccac gaagctggcg agcaggctcg acccgccgta gctcacgaag
     1741 ggcagggtga tgccggtcag gggcacgacc ttgaggttgc cgccgatgat gatcaggctc
     1801 tggagcacga gaccggcggt gaggccggcg gccagcatcg tgctgaagtc gtcgcgcgcg
     1861 aggatcgcga tccggagccc gcgggccatg accacggcca ccaggccgag cagcgcaacg
     1921 ccgccgacga gcccgagctc ctcgccgatg gcggcgaaga tcatgtcggt gtggacggcg
     1981 ggaatcggca gtaccccatt gatggtcggc aacccctgac ccagcccctc cccgacgagc
     2041 ccgccgcggg cgaaggcgta cagcgcctgg atggcctggt agcccgagcc gctcgggtcg
     2101 gcccacgggt cgagccacgc gtcgatccga acctggacat gcgggaagag caggtacgcg
     2161 accgtcgcac cgaggacgaa caggatcatg ccgaacagga cgtaggcccg acgcccggtg
     2221 gccacgaaca gcatggtcag gaagatgccg aagaacatga gcgcggtgcc caggtcgcgg
     2281 ctgacgacca cgacgagcat caccgcggcg aacacgatga gcatgggcgc gaggtacggc
     2341 aggggtggca gggtcagcgg gccgagccgc agcgaggcgc cggccagcac ggcccggttt
     2401 tcagccaggt agccggcgag gaagacgacg agcatgacct tgatcgcctc ggacggctgg
     2461 aagccgaagg ggccgaccac gatccacagc cgcgcctggt tgatctcggt tccgaacagg
     2521 aaggtgatca ccaacaggag cgccgcgaac aggccccagc tgtacttgta gtgccgcagt
     2581 aagccgtcat cccgaagacc gatcgcgatc agcaccatcg cccccaggcc caccccgaac
     2641 cacaccagct gcgtgccgac catgcccagg ttgagcgccg cgttgccggc gaggtcctgg
     2701 ggcaggcggt tcagcatgat cacgcccagg gcgcccatcc cagctacggc cggcaggagc
     2761 agctcatcgc cgcggtggcc gtaccaggcc agcgcgaaat gggccacggc gacgagcccg
     2821 atgaacgcga tcgcgaccgc catcggtccg atttcggcgg tgtccgactg ggcgatgttg
     2881 gtcacgagca cgcccagtgg cacgagcagg aggatgggcg cgagcaggga aagctccgtc
     2941 cggcgcgcgg cgagccgata catgctcagc gctcgaggcg gaagcgcacg cggccgatgg
     3001 tcagctcgtc accaaaggtc agggcgaccg ggcgctcgat gcggtggccg ttgacccacg
     3061 tcccattcgt gcttccgcgg tcctcggcgt accacgagcg tccacggaac gtgagcacgg
     3121 catgcgtccc gctcgcgaac tcgtcgtcga ggtagatggt gctgttcacg tcgcggccga
     3181 gggtcgtgat cgggccgatg tgcagcacgc ggtcgacggc cggctcgccg ccgggcgact
     3241 cgagaactac gagccgcccg ataccggtgc gggatgccgc ctcgatcgcc gccgcctgcc
     3301 ggaggtctgc gcgcagggat cgcgcgaacc gaagcacgat caggaccagg agggcgagga
     3361 atccgagctg gagcgcgagg aggatcagcc gaaggatgtc gttggtcacc ggcagaggtt
     3421 ggtcatccgt gcagccggaa gtcgacgttc gtgttaccga tccggatccg gtcgccgtcg
     3481 gccagggcga tctccgcaac cgcatgcccg ttcacggtcg atccgttccg gctctggagg
     3541 tcgacgaacc cgtatgcgcc gtgctggacc ttgagctggc agtgatggcg actcacctgg
     3601 ggatcatcga ggatgacgtc gttgtcggtg gcccggccga tcgcgatcag cgcaccgccg
     3661 aggtcgaagc gggcggccgt gccgccatcg gtctggacca cgaggtaggc ccgtgaggcc
     3721 gtgttcgggt cgggtaccgc gtggcccggg cgggcgagga cgctggtctg gtcggccatc
     3781 tggcgctcga cgatgggcgc cgcgtcgttg tcggccaccg cggcagcgac ccggacgtcg
     3841 ccacgcggga cgaggccgtc ggcctgcagg atgacccgtg gccgagccat gagccggtac
     3901 ccctcgcgac gggatcgggt caggacctcg tgggcgaggt cgtcctcgag tgagccgtgg
     3961 tacgactcga acgaggcgta gtcggtgggg ctgagatgga cgacatagcg attcgggacg
     4021 atcacgccgt cctcgccgaa tcgcttgttc gtgtccatgg cgcgcgcgat ccgcttggcc
     4081 agggtcaccg gctgcggtgc cgctcccatg cgt
//
LOCUS       DASYGU010000049         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1036171, whole genome shotgun sequence.
ACCESSION   DASYGU010000049 DASYGU010000000
VERSION     DASYGU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036171"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGO32_01275"
     CDS             complement(<1..363)
                     /locus_tag="VGO32_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEV7199415.1"
                     /translation="MRTATHNLSVAGVIAAKDLRAELRGRQAVTSTLFLAAVTMLIFG
                     FALGPDEDRLAVAGPGLLWLAIILSGILAVGRLHHLETDDGAFELLGLYPISRTGIYL
                     GKALGGLAAMLVIGALVLP"
     gene            complement(360..1145)
                     /gene="ccmA"
                     /locus_tag="VGO32_01280"
     CDS             complement(360..1145)
                     /gene="ccmA"
                     /locus_tag="VGO32_01280"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEV7199416.1"
                     /translation="MPPTTIHVRPTGVPDETTPGVETRDLARLFRRSPALAGITIQVV
                     SGRAVAILGANGAGKTTLLRVLASALRPSYGSAAVDGIDVADRPEEIRPRVAFLPHAT
                     GLYDDLTAAENLGFAAALMNVPAPDRFDRVRAALDRVQLTAERDSRVGRFSAGMRRRL
                     ALGRILLGHPRLVLLDEPYAALDAEGMAIVDELLDEWRANGATVLVATHATERLGERM
                     DGWMRLENGLLVDLGGVGVSGRVAARPATDLPASPPLAAGFRS"
     gene            1235..1618
                     /locus_tag="VGO32_01285"
     CDS             1235..1618
                     /locus_tag="VGO32_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV7199417.1"
                     /translation="MPTPRSTRDIRASFGLVLVLVGTLSLAACSSPASSPASGADHTV
                     TIVGTSFGDDFTIPVGGTVVFVNDAGTNHTVTNGVDGEPNEDPLFDFELADGETSDPI
                     TFDDPGTYDVTCRIHHSMHLTITVE"
     gene            1736..2053
                     /locus_tag="VGO32_01290"
     CDS             1736..2053
                     /locus_tag="VGO32_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7199418.1"
                     /translation="MLASNGETAATAALTVLGQKWVLRIVHSLGERTQRFCELQDALG
                     GANSATLSQRLKLLEDEGLISRRVISSVPPWVEYSLTPKGRELHEALAPIDRWADRWA
                     TSA"
     gene            2064..>2506
                     /locus_tag="VGO32_01295"
     CDS             2064..>2506
                     /locus_tag="VGO32_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7199419.1"
                     /translation="MSHPVAEPAPGEHVDALALVRDTVAAAGSLDDASQAAVDVLHAR
                     FPHYDWVGIYWVDPSGTELVLGPWTGPEATEHTRIPIGTGICGAAAASGTTEIIDDVN
                     ADPRYLACFTTTRSEIVVPIFHEGVVIGEIDIDGSDEAAFGPTDAR"
BASE COUNT          386 a          909 c          862 g          349 t
ORIGIN      
        1 gggcagcacc agggcgccga tgaccagcat cgccgcgagc ccgcccagcg ctttgcccag
       61 atagatcccc gtccggctga tggggtacag ccccaggagc tcgaacgcgc cgtcatcggt
      121 ctcgaggtgg tggagccgac caacggccag gatcccgctc aggatgatgg ccagccacag
      181 gagcccaggc ccggccaccg ccaggcggtc ctcgtccgga ccgagcgcga acccgaagat
      241 gagcatggtc acggcggcca ggaagagggt ggaggtcacc gcctgccggc cccgcagctc
      301 ggcgcggagg tccttggcgg cgatcacgcc ggccaccgac aggttgtggg tggcggtcct
      361 cacgaccgga acccggcggc caggggcggc gaagcgggca ggtcggtggc cggccgggcc
      421 gcgacccgtc cgctgacgcc gactccaccg aggtcgacca agagcccgtt ctcgagtcgc
      481 atccagccgt ccatccgctc ccccagccgc tcggtggcgt gggtcgcgac caggaccgtg
      541 gctccgttcg cccgccactc gtcgagcagc tcgtcgacga tggccatgcc ctcggcgtcg
      601 agggccgcgt agggctcatc gaggagaaca agccgcgggt ggccgagcag gatccggccg
      661 agcgcgagac ggcggcgcat gccggccgag aatcggccaa cccggctgtc ccgctcggcg
      721 gtcagctgga cccggtcgag tgctgctcgg acacgatcaa accgatccgg ggcaggcacg
      781 ttcatgagcg cggcggcgaa gcccaggttc tcggcggcgg tgaggtcgtc gtacaggcct
      841 gtggcgtgtg gcaggaaggc gacccggggg cgaatctcct ccggccggtc ggcgacgtcg
      901 ataccgtcca ccgcggccga cccgtacgac ggccggagcg cgctggccag gacgcggaga
      961 agcgtggtct tgcccgctcc attggctccg agaatggcga ccgcccgacc cgaaaccacc
     1021 tggatcgtga tcccggccag cgcggggctg cgtcggaaga gccgggccag gtcgcgagtt
     1081 tcgactcccg gcgtggtctc gtcagggacc cccgttgggc gaacgtggat ggtggtcggc
     1141 ggcatgcctc atcccgtggg cggaggggcg ggtggcagca gtgtgcggcc tgccccacaa
     1201 ttgcgtcaaa tacgccgcag gagctccgca accgatgcct actccccgct cgacacgcga
     1261 tattcgcgcc tcattcggcc tcgtcctggt cctcgtcggc accctgtcgc tggcggcctg
     1321 cagcagtccc gcgtcctccc ccgccagcgg ggccgaccac acggtcacca tcgtcgggac
     1381 ctcgttcggc gatgacttca ccatcccggt gggtgggacg gtcgtgttcg tgaacgacgc
     1441 cgggaccaac cacacggtga ccaacggggt cgatggggag cccaacgagg atccgctgtt
     1501 cgacttcgag ctggccgatg gcgagaccag cgacccgatc acgttcgacg atcccggcac
     1561 gtacgacgtg acctgccgga tccaccacag catgcatctg accatcaccg tcgagtagtc
     1621 cgaaggccgg ccgccgagtt tgaccctccc cggggccggt gctaccgttc ggcgcctcat
     1681 ggacgctggc cttcaggcgc cgctgcccac accccacgga cgagcccatc gccgcatgct
     1741 ggcgagtaac ggtgagaccg cagcgacggc tgccctgacc gtcctcggcc agaagtgggt
     1801 cctgcgcatc gtccactccc tcggcgagcg gacccagcgc ttctgcgagc tgcaggatgc
     1861 cctcggcggc gcgaactcgg ccaccctttc ccagcggttg aagctcctcg aggatgaggg
     1921 gctcatctcg cgccgggtga tctccagcgt cccgccgtgg gtggagtact ccctgacccc
     1981 caagggtcgc gagctgcacg aggccctggc ccccatcgac cgctgggcgg accgatgggc
     2041 cacctcggcc tgatccccga acgatgagcc acccggtcgc cgagccggcg cccggagagc
     2101 acgtcgacgc cctcgccctc gtccgtgaca cggtcgcggc cgcgggcagc ctggacgatg
     2161 ccagccaggc ggcggtggac gtccttcacg cccgattccc gcattacgac tgggtcggca
     2221 tctactgggt cgacccgtct ggcaccgagc tcgtcctcgg gccgtggacc gggcccgagg
     2281 ccaccgagca cacccggatc ccgatcggaa ccggcatctg cggcgcggcc gcggcgagcg
     2341 gcacgaccga gatcatcgat gacgtgaatg ccgacccccg ctatctcgcc tgcttcacga
     2401 ccacccgctc ggagatcgtg gtcccgatct tccacgaggg cgtggtcatc ggcgagatcg
     2461 acatcgacgg ctcggacgag gcggcctttg ggccgaccga cgcgcg
//
LOCUS       DASYGU010000050        10138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_521883, whole genome shotgun sequence.
ACCESSION   DASYGU010000050 DASYGU010000000
VERSION     DASYGU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521883"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..489)
                     /locus_tag="VGO32_01300"
     CDS             complement(73..489)
                     /locus_tag="VGO32_01300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEV7199420.1"
                     /translation="MTRLLIHTDGAARGNPGPAGAGALLQDAETRETVAEIAEFLGER
                     TNNYAEWTAVELALIRARELDADAVDLRTDSELVARQISGRYRVKHPDLKPIHARVMT
                     LLNGFGAYTVGHVPRERNKDADRLSNVAIDDRPGSV"
     gene            complement(486..1187)
                     /locus_tag="VGO32_01305"
     CDS             complement(486..1187)
                     /locus_tag="VGO32_01305"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV7199421.1"
                     /translation="MQVELRIRRFNPEADEASHWESYDLEADPTDRVLDLLHQVKWYQ
                     DGTLTFRRSCAHGVCGSDAMLINGRNRLACATLVRDAGETISVEPMRGLPVVKDLLVD
                     QEEFWAKYRSAMPYLVNDEVPEDGLERRQSPFDRAKYEDTSRCILCAACTTACPVFWT
                     NPAYVGPAAIVNAHRFIFDSRDTAHEERLELMADADGVWRCRTVFNCVEACPRGINVT
                     RAILEVTQAIEDRPA"
     gene            complement(1191..2969)
                     /gene="sdhA"
                     /locus_tag="VGO32_01310"
     CDS             complement(1191..2969)
                     /gene="sdhA"
                     /locus_tag="VGO32_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEV7199422.1"
                     /translation="MVGRGPSAAPRIHVLDAIVVGAGGAGLYAALELKRGLGEAARIG
                     VVSKLYPTRSHTGAAQGGVCAALGNTEEDHWEWHWWDTVKGGDYLVDQDAAEVMCRDA
                     VQTIRDLEHLGLPFDRTPDGLIDQRRFGGHSRNHGEAPVRRSCYSADRTGHAILQTLY
                     QQCIRHEVAFFDEYQVLDLLMPDGPAGRVSGVLALQLSSGELTVFLAPAVLFATGGFG
                     MVYKVTSNAHTLTGDGVAIAYRRGLGLEDMEFFQFHPTGMYKLGFLLSEAMRGEGGIL
                     LNGEGERFMERYAPTMKDLASRDVVSRSIYQEVAAGRGVNGQDFVHLDVRHLGAEILD
                     HKLPDMAGFIRTYFGLEPLTDLVPIQPTAHYAMGGIPTDVDGRVLADVAGRVVEGFYA
                     AGECACVSVHGANRLGTNSLLDILVFGRRAGMAMTAQVRDAATPTRDLEAEARAAGRL
                     ADLLGSTSGERPATIRTELREMMFRDCGVYRSAAGLASAATAVASLRERAQRLRLDDH
                     GSRYNTDLTDALELGYLLDCAEAMVTSAAARTESRGGHAREDFPDRDDDHWMRHTFAT
                     RDGKGTVALTYKPVTVTEFQPAPRVY"
     gene            complement(2992..3432)
                     /locus_tag="VGO32_01315"
     CDS             complement(2992..3432)
                     /locus_tag="VGO32_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEV7199423.1"
                     /translation="MSVGRTVWLGNPPARRARPPAGSGRERFWWWFMRLSGLALVILA
                     LGHMFIMHVLVQLSGQEISWAFVTSRWGTPFWRIYDALLLVLALIHGVNGARVVISDL
                     VPRGGIRAVLVGGLALLAFAWLVLGLMVVVFFNPETAPPVGPFS"
     gene            complement(3437..3835)
                     /gene="sdhC"
                     /locus_tag="VGO32_01320"
     CDS             complement(3437..3835)
                     /gene="sdhC"
                     /locus_tag="VGO32_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV7199424.1"
                     /translation="MTVGSWLAQAQARVVGYRVSWSQLAWFGHRLSGLGVLLYLFMHI
                     VETSTVVLGPDVYNTTIGLFRNPVIRAAEILLMAALVYHSLNGLKIILIDFVPAATAW
                     YRPLTYGVIIATVVAMVPMSLIMFAPFAPW"
     gene            complement(3832..4857)
                     /locus_tag="VGO32_01325"
     CDS             complement(3832..4857)
                     /locus_tag="VGO32_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEV7199425.1"
                     /translation="MTYEVTLIPGDGIGPEIAAATCEVLEATGLDFRWDEQTAGEATL
                     TSEGNPLPDRVVESIRRTRVALKGPITTPVGTGFRSVNVALRQALTLYASVRPARSIR
                     GIESRYENVDLVIVRENTEDLYAGFERSISEDEAESIKIITRKASVRIARYAFEYAVA
                     NGREMVTAVHKANIMKLTDGLFLECCRTVAAEYEGRIGFQDRIVDNMCMQLVQKPETY
                     DVLVAPNLYGDIISDLAAGLVGGLGIAPGANIGEDAAVFEAVHGSAPKYAGQDRANPT
                     ALILSGALLLRHIGETDAGVAVEQAVREIIGEGSKVTADLGGRTGTRAYGKAVAGRVR
                     ALRGAAA"
     gene            4931..6076
                     /gene="sucC"
                     /locus_tag="VGO32_01330"
     CDS             4931..6076
                     /gene="sucC"
                     /locus_tag="VGO32_01330"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEV7199426.1"
                     /translation="MKLQEFRSKEILARHGIPTLPGEVTTTPAGARTIAERLAVPVVV
                     KAQVLAGGRGKAGGVKLAATPDEAEARATDILGLTIAGGPVRTVLVTPAVDIGHEYYL
                     SAVLDRGAKAVTIIASAEGGIEIEEVAHSQPDAVLRHSLDPLAGLQPADVETVTAFMG
                     IPEVHRSTFGSILEGLVAAFFGSDAELAEVNPLAVTGDGRLLALDAKIVVDDSALPRH
                     PDIEAMRDLSEEEPAEIEAREAGISYIKLDGTIGCMVNGAGLAMTTMDLVKLAGGEPA
                     NFLDIGGGARHERVAAAFGIILADPNVRAVLINIFGGITRGDEVAHGIVEARAGLDRE
                     VPMVVRIVGTNADEAALILEQANLQIAYSLDEAAELAVAAAGAGGSR"
     gene            6073..6948
                     /gene="sucD"
                     /locus_tag="VGO32_01335"
     CDS             6073..6948
                     /gene="sucD"
                     /locus_tag="VGO32_01335"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEV7199427.1"
                     /translation="MSILVNQETRLLIQGITGREGQFHGQAMLDYGTPVVSGVTPGKG
                     GQRTFNNRVPVFNTVEEAIRETGANTSVIYVPAAFAPDAMLEAAAAGIGLIFCITEGI
                     PALDMLRVYHTLRQQGVRLIGPNCPGATSVGQAKVGIIPGNIHRRGTVGVVSRSGTLT
                     YEVVQALTDEGLGQSTCVGIGGDPIIGSNFIEILDLFEADPETDAVVLIGEIGGDEEE
                     RAAAHIAEHLSKPVTAFIAGRTAPPGRRMGHAGAIISGGAGTAESKREALRKAGVRVA
                     DSPLQIPALLRGPVG"
     gene            complement(6945..7400)
                     /locus_tag="VGO32_01340"
     CDS             complement(6945..7400)
                     /locus_tag="VGO32_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7199428.1"
                     /translation="MSDERVVPTGLGPLDHVAIVVRDIDESLPRYQALFGMVAPDGVQ
                     VVTAQAVRICFLPTGPPPSARIELIQPLDAESGVARFLASRGEGLHHVCFGTSDLTAE
                     LDRLEAAGAELIDHAPRPGAEGPVAFVHPRTLNGVLWELLQTGGPPEDG"
     gene            complement(7397..9022)
                     /locus_tag="VGO32_01345"
     CDS             complement(7397..9022)
                     /locus_tag="VGO32_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV7199429.1"
                     /translation="MTRGDDPGDPAGRTPRHDRTTSGISIGDVYRSGDVAGGLEDRLG
                     EPGAYPYTRGIHASGYRGRPWTMRQYAGFTSAEESNRRYRDLLAGGQTGLSVAFDLPT
                     QMGYDSDDPRVAGEVGRVGVPIDSVHDMERLLDGIDLGTVSTSMTINATAAILLALYE
                     AVGRRQGIEPGRLRGTVQNDILKEYVARGTYIFPPEPSLRLATDLIAYCRAELPHFNP
                     ISISGYHMREAGATAVQELAFTFGNAITYADAAIARGLSFDDFAPRLSFFFASFNDLF
                     EEVAKFRAARRIWARVARERYGATDPRSMTLRFHVQTAGSTLTAQQPETNTVRTTVQA
                     LAAVLGGAQSLHTNALDEALGLPSPEAARLALRTQQVLAHESGLTAPIDPLGGSWYVE
                     ALTDALDERATAELARLDELGGPLVALESGYQANAIADAAYADQQAFESGEAVVVGVN
                     AFTDADEDGDAGVRPQPQFIDPAAEQLQAQRTAAVRAARDPATAADALAALGSAARGT
                     ENLMPRIRACVEADVTLGEIADGLRAAWGEHRP"
     gene            complement(9033..9521)
                     /locus_tag="VGO32_01350"
     CDS             complement(9033..9521)
                     /locus_tag="VGO32_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEV7199430.1"
                     /translation="MTDDPQAGEILVEGSATTPAQAVPLSWVDRDRGLGFVHHGPRPA
                     LVVVDGGPTEWVVTIRGRRIAVSVHSRRDQLIASAQQQAGAQRGPMEVRATLPGLVLH
                     VLVEVGQVVDAGDPLVTIEAMKMQNEIRAPRAGRVASIAVAAGQTLAAGASLVQLVDP
                     EP"
     gene            complement(9518..>10138)
                     /locus_tag="VGO32_01355"
     CDS             complement(9518..>10138)
                     /locus_tag="VGO32_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199431.1"
                     /translation="ELVTGLDLVACQLRIAAGEVLPPEIMDPGLRGHAIEVRVYAEDP
                     WNGFGPTAGRIGAWRMPDGPGVRVDAGVEAGYELPTAYDPLLAKIMVHAADRDASVAR
                     LRRALDETGIGGMATTLGFHRWLVDQPTFTEGGYDTDFVTDAWAGGPSLTADDATLAA
                     AVVTATRLGGGHRRRDPLPPDEAPEGWAADGERAWSRLARTEAVEP"
BASE COUNT         1504 a         3557 c         3584 g         1493 t
ORIGIN      
        1 cggactttcc tcgaacgccg cctttcggcg atcgcgtccg cggccgtctg gccgcctcca
       61 gggagcgcga gtctagaccg atcccggtcg gtcgtcgatg gcgacgttgc tcagccgatc
      121 ggcgtccttg tttcgctcgc gtgggacgtg gccgaccgtg taggcgccaa agccgttcag
      181 gagggtcatc acccgggcat ggatgggctt gagatccgga tgcttgaccc ggtagcgacc
      241 cgagatctgg cgggcaacga gctccgaatc ggtccgcagg tcgaccgcat ccgcgtcgag
      301 ctctcgcgcc cggatgagcg ccagctcgac cgccgtccac tccgcgtagt tgttcgtccg
      361 ctctccgagg aactcggcga tctcggccac cgtctcccgc gtctccgcgt cctggagcag
      421 ggcccccgcg cctgccggtc cggggttgcc gcgtgcggcg ccatcggtgt ggatcagcag
      481 gcgggtcaag cgggccggtc ctcgatggcc tgggtcacct ccaggatcgc gcgcgtgacg
      541 ttgatccccc gcgggcatgc ctcgacgcag ttgaacaccg tgcggcagcg ccagacccca
      601 tccgcatcgg ccatcagctc gaggcgctcc tcgtgggcgg tgtcgcggct gtcgaaaatg
      661 aaccgatggg cgttcacgat ggccgccggg cccacgtacg ccggattcgt ccagaacacc
      721 gggcacgccg tggtgcaggc ggcgcacagg atgcagcggc tggtgtcctc gtacttcgcc
      781 cggtcgaagg gcgactgtcg ccgctccagg ccgtcctcgg gcacctcgtc gttcacgaga
      841 tacggcatcg cgctccggta cttggcccag aactcttcct ggtcgacgag gaggtccttg
      901 accaccggca ggccgcgcat cggctcgacc gaaatggtct cgccggcgtc gcgaacgagc
      961 gtcgcgcagg ccagccggtt gcggccgttg atgagcatgg catcggaccc gcacacgccg
     1021 tgagcgcagg agcggcggaa ggtgagtgtc ccgtcctggt accacttcac ctggtgcaga
     1081 aggtcgagca cccggtcggt cggatcggcc tcgaggtcgt acgactccca gtgcgacgcc
     1141 tcgtcggcct ccgggttgaa gcggcggatc cgcagctcga cctgcaccgg tcagtacacc
     1201 cgcggtgccg gctggaactc ggtcaccgtc accggcttgt aggtcagggc cacggtgccc
     1261 ttgccatcgc gtgtggcgaa ggtgtggcgc atccagtggt cgtcgtcgcg gtccgggaag
     1321 tcttcccgcg catggccacc gcggctctcg gtccgggccg cggccgaggt caccatcgcc
     1381 tcggcgcagt cgagcagata gccgagctcg agggcatcgg tcaggtcggt gttgtaccgg
     1441 ctgccgtggt cgtcgagccg gaggcgctgg gctcgctcgc ggagggaggc cacggccgtg
     1501 gcggccgatg ccaggccagc cgccgatcgg tacacgccgc agtcgcggaa catcatctcg
     1561 cggagttcgg tccggatggt tgccggccgc tcgccgctgg tgctgcccag gaggtccgcc
     1621 agccggcccg cggcgcgcgc ttcggcctcc aggtcacggg tcggggtcgc ggcatcgcgg
     1681 acctgggcgg tcatggccat cccggcccgg cgaccgaaga cgagaatgtc gagcagcgag
     1741 ttcgtcccca gccggttcgc gccatggacg ctgacgcagg cgcattcgcc cgcggcgtag
     1801 aagccctcca ccacccgacc ggcgacgtcg gccagcaccc ggccatccac gtcggtcggg
     1861 atgccgccca tggcgtagtg ggccgtgggc tggatgggca ccaggtcggt cagcggctcg
     1921 aggccgaagt aggtccggat gaagccggcc atgtccggca gcttgtggtc caggatctcg
     1981 gcgccgagat gacggacgtc gagatggacg aagtcctggc cgttcacccc gcgcccggca
     2041 gccacctcct ggtagatgga ccggctgacc acgtcccggc tggcgaggtc cttcatcgtc
     2101 ggtgcgtagc gctccatgaa ccgttcgccc tcgccgttga ggagaatgcc cccttcgccg
     2161 cgcatcgcct ccgacaggag gaagccgagc ttgtacatgc cggtcgggtg gaactggaag
     2221 aactccatat cctccaggcc gaggccgcgt cggtaggcaa tcgccacccc atcgccggtc
     2281 agggtgtggg catttgaggt gaccttgtag accatcccga atccgccggt cgcgaacaga
     2341 acggcgggag caaggaacac ggtcagctcg ccgctcgaga gctgcagcgc cagcaccccg
     2401 ctcacccgac ccgcgggtcc gtcgggcatg agcaggtcca ggacctggta ctcatcgaag
     2461 aacgcgacct cgtggcggat gcactgctgg tacagggtct ggaggatggc gtggccggtg
     2521 cggtcggcgg agtagcacga ccggcgaacc ggtgcctcac cgtggttccg agagtgcccc
     2581 ccgaaccgac gctggtcgat caggccgtcg ggcgtgcggt cgaacggcag gcccaggtgc
     2641 tcgaggtcgc ggatggtctg gaccgcgtcg cggcacatga cctccgccgc gtcctgatcg
     2701 acgaggtagt ccccgccctt gaccgtgtcc caccagtgcc actcccagtg gtcctcctcg
     2761 gtgttgccca gtgcggcgca gaccccgccc tgggcggcac cggtgtggga gcgagtcggg
     2821 tacagcttgg aaacgacgcc gatccgcgcc gcttccccga ggccgcgctt cagctcgagg
     2881 gcggcataca ggcccgctcc cccggcaccg accacgatcg cgtccaggac gtggatgcgc
     2941 ggcgccgcgg acgggccgcg gccgaccatg gggacagtca cgccgggcag ctcatgagaa
     3001 gggcccgacc ggcggcgcgg tttccgggtt gaagaacacg acgaccatca ggcccaggac
     3061 cagccaggcg aacgccagca gggcgaggcc accgaccagc acggcccgga tccctccgcg
     3121 cggaacgagg tcgctgatca caacccgggc gccgttcacg ccatggatga gggcgagcac
     3181 gagcagcagc gcgtcgtaga tccgccagaa gggagtcccc caccggctgg tcacgaacgc
     3241 ccagctgatc tcctggcccg agagctggac cagcacatgc atgatgaaca tgtggcccag
     3301 ggcaaggatc accagggcca gcccgctcag ccgcatgaac caccaccaga accgctcgcg
     3361 cccagagccg gccggcggtc gcgcgcggcg cgccggcggg tttccgagcc agacggtccg
     3421 acccacgctc atcggatcac cacggcgcga agggggcgaa catgatcagg gacatgggca
     3481 ccatggccac caccgtggcg atgatcacgc cataggtcag cggccgatac cacgccgtcg
     3541 cagccggcac gaagtcgatg aggatgatct tcagcccgtt cagggagtgg tacacgagcg
     3601 cggccatgag caggatctcg gcggcgcgga tgaccgggtt ccggaacaag ccgatggtgg
     3661 tgttgtagac gtccggcccg aggaccacgg tcgaggtctc gacgatgtgc atgaagaggt
     3721 agagcaggac gccgagaccc gacagccgat ggccgaacca ggcgagctgc gaccagctga
     3781 cccggtagcc gaccacccgg gcctgcgcct gggccagcca cgaaccgacg gtcatgcggc
     3841 cgcccctcgg agcgcacgga cccgcccggc cacggccttc ccataggccc gcgtcccggt
     3901 cctgccgccg aggtcggcag tcaccttcga tccctcgccg atgatctcgc ggacggcctg
     3961 ctccaccgcg acgccggcat cggtctcgcc gatgtggcgc aggaggaggg ctccggacag
     4021 gatcagggcg gtggggttcg cgcggtcctg gccggcgtac ttgggggcgg agccgtggac
     4081 ggcctcgaag accgccgcgt cctccccgat gttcgctccc ggggcgatcc cgagcccgcc
     4141 gaccagcccg gcggcgaggt cgctgatgat gtccccgtac aggttcggag ccaccagcac
     4201 gtcatacgtt tccggcttct ggacgagctg catgcacatg ttgtccacga tccggtcctg
     4261 gaacccgatc cgaccctcgt actcggccgc gaccgtgcgg cagcactcca ggaacagccc
     4321 gtcggtcagc ttcatgatgt tggccttgtg gacggcggtc accatctcgc gtccgttggc
     4381 caccgcatac tcgaacgcgt agcgggcgat ccggaccgat gccttccggg tgatgatctt
     4441 gatgctctcc gcctcgtcct cgctgatcga acgctcgaag cccgcgtaca ggtcttcggt
     4501 gttctcccgg acgatgacga ggtccacatt ctcgtaccgc gactcgatgc cgcggatgct
     4561 tcgggcgggc cggacgcttg cgtacagggt cagcgcctgg cggagcgcga cgttcacgct
     4621 ccggaacccg gtacccaccg gggtcgtgat cggccccttg agcgcgaccc tcgtccggcg
     4681 aatgctctcg acgactcggt ctgggagcgg gttgccctcg ctggtgagtg tcgcctcgcc
     4741 ggctgtctgc tcgtcccagc ggaagtccag tccggttgcc tcgagcacct cgcacgtggc
     4801 agccgctatc tcggggccaa tcccgtcacc ggggatcagg gtcacctcat aggtcacgac
     4861 ccaagtgtag gcagccgcga ccgcagccgg tggccggtcg acagcggggc tggtcgggta
     4921 cgctcggggc gtgaagctcc aggagttccg cagcaaggaa atcctcgccc gccacgggat
     4981 tccgaccctc cccggcgagg tgacgactac cccggcgggc gcccggacga tcgccgagcg
     5041 gctcgccgtg ccggtcgtgg tcaaggccca ggtcctggct ggcggccgag gcaaggcagg
     5101 tggtgtgaag ctggcggcca cgcccgacga ggcggaggcc cgggcgaccg acatcctggg
     5161 gctgaccatc gccggggggc cggtgcggac cgtgctcgtc acgccggccg tcgacatcgg
     5221 acacgagtac tacctgtcgg cggtgctcga tcgtggcgcg aaggcggtga ccatcatcgc
     5281 cagcgcggag ggtggaatcg agatcgagga ggtcgcccac agccagccgg acgccgtcct
     5341 ccgccactcc ctcgacccgc tggccgggct ccagcccgca gatgtcgaga ccgtgaccgc
     5401 gttcatggga atcccggagg tccatcggtc caccttcggc tccatcctcg agggcctggt
     5461 cgccgcgttc ttcggctcgg atgccgagct ggccgaggtc aacccgctgg cggtgaccgg
     5521 tgacggccgg ctgctcgctc tcgacgccaa gatcgtggtc gacgactccg cgctgccccg
     5581 acacccggac atcgaggcca tgcgcgatct gtccgaggag gagccggccg agatcgaggc
     5641 gcgcgaggcg ggtatcagct acatcaagct ggacgggacg atcggctgca tggtgaacgg
     5701 cgccgggctg gcgatgacga cgatggatct cgtcaagctg gccggcggag agccggcgaa
     5761 ctttcttgac atcggtggcg gcgcccgcca cgagcgcgtc gcggccgcct tcggcatcat
     5821 cctggccgat ccgaacgttc gcgccgtgct gatcaacatc ttcggcggga tcacccgcgg
     5881 cgacgaggtg gcccacggca tcgtcgaggc ccgggccgga ctggaccgcg aggtcccgat
     5941 ggtcgtccgg atcgtgggca ccaacgccga tgaggcggcc ctcatcctcg agcaggcaaa
     6001 cctccagatc gcgtacagcc tggacgaggc cgccgagctg gcggtggcgg cggccggggc
     6061 cggtggctcc cggtgagcat cctcgtcaac caggaaacgc ggctcctcat ccagggcatc
     6121 accggccgcg agggccagtt ccatggccag gccatgctcg actacgggac gccggtggtt
     6181 tcgggtgtca cgccggggaa gggcgggcaa cggacgttca acaaccgggt acctgtgttc
     6241 aacacggtcg aggaggcgat ccgcgagacc ggggccaaca cgagcgtcat ctacgtcccg
     6301 gcggccttcg cccccgacgc gatgctcgag gcggcggcgg ccggtatcgg cctcatcttc
     6361 tgcatcaccg agggcatccc ggccctggac atgctgcgcg tctaccacac cctccgccag
     6421 cagggcgtcc ggctgatcgg gcccaactgt cccggtgcca cgtccgtcgg ccaggccaag
     6481 gtcgggatca tcccgggcaa catccaccgg cgcggcacgg tcggtgtggt cagccgctcg
     6541 ggaacgctga cctacgaggt ggtccaggcc ctgaccgatg agggacttgg ccagtcgacc
     6601 tgcgtcggca tcggcggtga tccgatcatc ggctccaact tcatcgagat cctcgacctg
     6661 ttcgaggctg acccggagac cgacgcggtc gtgctcatcg gagagatcgg tggcgacgag
     6721 gaggagcggg ccgcggcgca catcgcggag cacctgtcca agccggtgac cgcgttcatc
     6781 gccggacgca cggccccacc gggccgccgg atgggccacg cgggcgcgat catctccggc
     6841 ggagccggca cggccgagtc gaagcgagag gcgcttcgga aggcgggcgt ccgggtcgcg
     6901 gattcgccgc tccagattcc ggcgctgctc cgcgggccgg tcggctagcc gtcttcgggc
     6961 ggaccgcccg tctggagcag ctcccagagc acgccgttca gcgtccgcgg atggacgaac
     7021 gcgaccgggc cttcggcccc tggtcggggc gcgtggtcga tgagctccgc accggccgcc
     7081 tcgaggcgat ccagttccgc ggtgagatcg gaggtcccga agcagacgtg gtgcaggccc
     7141 tccccgcggc tcgcgaggaa gcgggcgaca ccgctctcgg cgtcgagggg ctggatgagc
     7201 tcgatcctgg cggatggcgg cggaccggtg ggcaggaagc agatccgaac ggcctgggcg
     7261 gtcacgacct gcaccccgtc cggcgcgacc atcccgaaca gcgcctggta gcgcggcagc
     7321 gactcgtcga tgtcgcggac cacgatcgcg acgtggtcca gcggaccgag cccggttggg
     7381 accacccgct cgtcgctcac ggccggtgct cgccccacgc cgcgcgcagc ccgtcggcga
     7441 tctcgcccag ggtcacgtcc gcctccacgc aggcccggat tcggggcatg aggttctccg
     7501 tcccgcgcgc cgcggagccg agcgcggcga gcgcgtcggc agcggtggcc gggtcccggg
     7561 ccgcgcgcac ggccgccgtg cgctgggcct ggagctgctc ggcggcggga tcgatgaact
     7621 gcggctgggg tcggacgcca gcgtccccgt cctcatcggc atcggtaaac gcgttcacgc
     7681 ccaccaccac ggcctcgccg gattcgaagg cctgctgatc ggcgtacgcc gcgtccgcga
     7741 tcgcgttcgc ctggtatccc gactcgaggg caaccagcgg cccgcccagc tcgtccagcc
     7801 gggccagctc ggccgtcgct cgctcgtcga gcgcatcggt cagggcctcg acgtaccacg
     7861 agcctcccag cgggtcgatg ggcgcggtga gcccgctctc gtgggccagc acctgctggg
     7921 tgcggagggc cagccgggca gcctcggggc tgggcaggcc cagcgcctcg tccagggcgt
     7981 tggtatggag tgactgcgcg ccgcccagga ccgccgccag cgcctggacc gtggtccgca
     8041 ccgtgttcgt ctccggctgc tgcgcggtca gagtggaccc cgccgtctgg acgtggaagc
     8101 ggagggtcat gctccgcggg tcggtggcgc cgtagcgttc gcgtgcgacg cgcgcccaga
     8161 tgcgccgtgc ggcgcggaac ttggccactt cctcgaacag gtcgttgaac gaggcaaaga
     8221 agaaggacag gcgcggggcg aagtcgtcga aggagaggcc tcgggcaatg gcggcatcgg
     8281 cgtaggtgat cgcgttgccg aaggtgaatg ccagctcctg gaccgcggtc gccccggcct
     8341 cacgcatgtg gtagccgctg atgctgatcg ggttgaagtg gggcagctcg gcccggcaat
     8401 aggcgatcag gtcggtggcc agccgcagcg acggctccgg cgggaagatg tacgtcccgc
     8461 gcgcgacgta ctccttgagg atgtcgttct ggaccgttcc gcgcaggcgt ccgggctcga
     8521 tcccctgccg tcgcccgacc gcctcgtaca gggccagcag gatggcggcc gtggcgttga
     8581 tcgtcatcga ggtgctgacc gtgcccaggt cgatgccgtc gagcagccgc tccatgtcgt
     8641 ggaccgagtc aatgggcaca ccgacccggc ccacctcgcc ggccacccgc gggtcgtcgc
     8701 tgtcgtagcc catctgggtc ggcaggtcga atgccaccga gaggccggtc tgccctccgg
     8761 caaggaggtc gcggtaccgg cggttcgact cctcggccga ggtgaagccg gcgtactggc
     8821 gcatcgtcca cggccgtccg cggtagcccg aggcatggat gccacgggtg tagggatagg
     8881 caccaggctc gccgagccga tcctcgagcc cgccggcgac atcgccggac cgatagacgt
     8941 cgccgatgga gatgcccgag gtggttcgat cgtgccgcgg cgtgcggccc gcggggtctc
     9001 ccggatcgtc gcccctggtc acgccgccga ttctacggtt cgggatcgac cagctggacg
     9061 agcgaggcgc cggcggccag tgtctgaccg gcggccaccg caatggacgc cacgcgcccg
     9121 gctcgtggcg cgcggatctc gttctgcatc ttcatggcct cgatcgtgac cagcgggtcg
     9181 ccggcatcga ccacctgccc gacctcgacc aggacgtgga ggacgagccc gggaagcgtg
     9241 gcccgcacct ccatcggccc gcgctgcgca ccggcctgct gttgcgcgga ggcgatgagc
     9301 tggtcccggc gggaatggac gctgaccgca atccgccggc cgcggatggt gaccacccat
     9361 tcggtcggtc ccccgtcgac caccacgagg gctgggcgcg gtccgtggtg cacgaagccc
     9421 agcccacggt cgcgatccac ccagctgagt ggtaccgcct gtgcgggggt cgtggcgctg
     9481 ccctcgacga ggatctcgcc cgcctgcgga tcgtcggtca cggctcgacc gcctccgtcc
     9541 tcgccagccg gctccatgcg cgctcaccgt cggcggccca accctcgggt gcctcgtcgg
     9601 gcggcaacgg gtctcgcctt cggtgcccgc cgccgaggcg ggtcgcggtc accacggcgg
     9661 ccgccagcgt cgcgtcatcg gcggtcagcg acggacctcc cgcccaggca tcggtcacga
     9721 agtcggtgtc gtacccaccc tcagtgaacg tgggctggtc cacgagccac cgatggaagc
     9781 cgagcgtggt cgccatcccg ccgatgccgg tctcgtccag ggcgcggcgg agtcgggcca
     9841 cgctcgcatc gcggtccgcc gcgtggacca tgatcttggc caggagcggg tcgtaggcgg
     9901 tgggaagctc gtagccagcc tccaccccgg cgtccacccg cacgcctggc ccgtcgggca
     9961 tgcgccatgc gccgatccgg cccgcggttg gaccgaaccc gttccacgga tcctcggcgt
    10021 agacgcggac ctcgatagca tggccgcgaa ggcccggatc catgatctcc ggcgggagga
    10081 cctccccggc cgcgatgcgg agctggcagg ccacgaggtc cagtccggtg accagctc
//
LOCUS       DASYGU010000051         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1396240, whole genome shotgun sequence.
ACCESSION   DASYGU010000051 DASYGU010000000
VERSION     DASYGU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396240"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VGO32_01360"
     CDS             <1..939
                     /locus_tag="VGO32_01360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEV7199432.1"
                     /translation="LTGSTVFTEIATVFSGGLMNGAVLVALGFLTVGATFKAAAVPFH
                     FWTPDAYQGAPTPITGFLSVGPKLGAFALLLRLFTEALGPLRADWLDVFVVLAVVTMT
                     FGNIVALTQTNVKRMLAYSSIAHTGYIIAGLAAYAAAPTPEVATQAVEAVVFYLVGYS
                     VMNLAAFAVVGMLQRDVRRYGGLASFAGLASRQPALAAAMTVLLLSLTGIPPTVGFFA
                     KLYILLSTVDAGLVWLAVAIALNAALAAFYYLRVVVYMYMRDPEAEPAEIERSGVGRL
                     ALVLSVAGVLLLGIFPGILLGWLPVEWVTAASGLVP"
     gene            1148..1657
                     /locus_tag="VGO32_01365"
     CDS             1148..1657
                     /locus_tag="VGO32_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEV7199433.1"
                     /translation="MAATPAPTAIPADWVATQLQIESVGINVAVQRLPSSQPLGTCCA
                     FLLPNTSDPGRGTNSYIAGHALNTLLKGLWNVQLGAEVRVLMSDGLVLRYRVTEVRPN
                     VSCPDPDQPPMTVTPPLALQHAPPGCVEGARWTAATDHEQLTLQTSQGFNRNWGELVV
                     IALPIEEPT"
     gene            complement(1680..>2381)
                     /locus_tag="VGO32_01370"
     CDS             complement(1680..>2381)
                     /locus_tag="VGO32_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199434.1"
                     /translation="VILGVHVIRQLWIQLRLLGLLALPPLAAIVAVIVQGQLGPVAGP
                     QPARLTLAIGFAVAAVLSAALIGTGFSEEVRSGAAAWLVVRAVPRTALIGAWLVVPSL
                     AVVVAYALAGVLAGLALPPTGQSADPVAIFISVVAAAAPAIPLAAAAITIGVNAPGRV
                     TALATVIGAVVLAIPLVLLGQAAVHPGSGYWLVAGIVPGDRPLTVGLQSIGLCLALAA
                     LAWFVAARRFLRRDL"
BASE COUNT          335 a          880 c          811 g          355 t
ORIGIN      
        1 ctgaccgggt cgaccgtgtt caccgagatc gcgaccgtgt tctcgggcgg gctcatgaac
       61 ggcgcggtgc tcgtcgcgct cggcttcctg accgtgggcg ccacgttcaa ggccgccgcg
      121 gtgccattcc atttctggac cccggacgcg taccagggag ccccgacgcc gatcacgggc
      181 ttcctgtcgg tcggccccaa actcggcgcc tttgccctgc tcctccgcct gttcaccgag
      241 gcgctcgggc cgctgcgcgc ggactggctg gacgtgttcg tggtcttggc cgtggtgacg
      301 atgaccttcg gcaacatcgt ggccctgacc cagacgaacg tgaagcggat gctcgcctac
      361 tcgtccatcg cgcacaccgg ctacatcatt gccggcctcg ccgcgtatgc cgcggccccc
      421 accccggaag tggccaccca ggcggtcgag gcggtcgtgt tctacctggt cggctactcg
      481 gtcatgaacc tggccgcctt cgcggtggtg ggcatgctcc agcgggacgt gcgtcgctac
      541 ggcggcctcg cgtcattcgc cggtctcgca tcgcgccagc cggcgctcgc tgcggcgatg
      601 accgtcctcc tgctgtcgtt gaccgggatc ccgcccaccg tcggcttctt cgccaagctg
      661 tacatcctgc tgtccacggt cgatgcgggg ctcgtctggc tggcggtggc catcgccctg
      721 aacgcggcgc tggcggcgtt ctactacctg cgggtggtgg tctatatgta catgcgcgat
      781 ccggaggccg agccggccga gatcgaacga tccggagtcg gccgcctcgc cctggtgctc
      841 agcgtggccg gtgtcctcct cctcgggatc ttccccggca tcctgctcgg ctggcttccg
      901 gtcgagtggg tgaccgccgc ctccggcctg gtgccatgag ggacccctcc tgaacgcgcg
      961 ctcccgcggg ctcgcccttc cgctgaccct ggcggtcctg gggatcgccc tgatcctggt
     1021 cggccagctg ccgctggacg agaccgcgac ccccagcctg ccgcccatcg tcacgccgac
     1081 cgccaccccg aacccaaccc ccgagcccac ggccgcggct tcgctgtctc cgtctgtgtc
     1141 tccgtcggtg gctgccaccc cagcgcccac cgcgattccc gccgactggg tcgcgaccca
     1201 gctccagatc gagtcggtcg ggatcaatgt cgcggtccag cgcctcccgt ccagccagcc
     1261 gctggggacg tgctgtgcat tcctcctgcc caacacctcg gaccccggtc gcgggacgaa
     1321 ctcatacatc gccggtcatg ccctgaacac gctcctcaag ggtctgtgga acgtccagct
     1381 gggggccgag gtgcgggtcc tgatgtcgga cggcctggtc ctccgctacc gggtgaccga
     1441 ggttcggccg aacgtgtcat gcccggatcc cgaccagccg ccgatgaccg tcacgccgcc
     1501 cctcgccctc cagcacgcgc cgccgggctg cgtggagggc gcgcgctgga cggcggccac
     1561 cgaccatgag cagctcacgc tccagacctc gcagggcttc aaccggaact ggggcgagct
     1621 ggtcgtcatc gccctgccga tcgaggagcc gacctaggtc agactcgctc cggcgggagt
     1681 cagaggtcgc ggcgcaggaa ccggcgggcg gcaacgaacc aggccagggc tgccagggcc
     1741 aggcacagac cgatggactg cagcccgacc gtgagcggac gatcgccggg cacgatcccg
     1801 gccaccagcc agtagccgct gcccggatgg accgcggctt ggccgaggag caccaatggg
     1861 atggccagca cgaccgcgcc gataacggtc gccagcgccg tcacccggcc gggcgcgttg
     1921 accccgatcg tgatcgcggc cgcggcgagc gggatggcag gcgccgccgc agccaccacg
     1981 ctgatgaaga tcgccaccgg gtcggcggat tgccctgttg gcgggagcgc gagtccggcc
     2041 aggaccccgg ccagggcgta ggccacgacc acggccaggc tgggcaccac gagccaggcg
     2101 ccgatgagcg cggtgcgcgg gaccgcccgg acgaccagcc atgccgcggc gccggatcga
     2161 acctcctcgc tgaagccggt cccgatcaag gcggcgctca gcacggcggc gacggcgaag
     2221 ccaatggcga gggtgagtcg ggcgggttgg ggacccgcga cggggccgag ctggccctgc
     2281 acgatcacgg ccacgatcgc ggcaagggga ggcagggcca ggagaccaag cagccgcagc
     2341 tggatccaca gctggcgaat gacatggacg cccaggatca c
//
LOCUS       DASYGU010000052         7480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_933584, whole genome shotgun sequence.
ACCESSION   DASYGU010000052 DASYGU010000000
VERSION     DASYGU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933584"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..156
                     /locus_tag="VGO32_01375"
     CDS             22..156
                     /locus_tag="VGO32_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199435.1"
                     /translation="MPATYRDTDAPLVTQVSDYVSGMTDRFAQAASDRLFGAGPSRSG
                     "
     gene            complement(148..510)
                     /locus_tag="VGO32_01380"
     CDS             complement(148..510)
                     /locus_tag="VGO32_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199436.1"
                     /translation="MNWRILGCGLLAAAVFIGLGLLALNLAIGRAGCPPTIQWGDRLY
                     DAVGEPTNVPVVGDGEPVLLGFTVAGALTREVYGPEGSTPSADPGHQPHEIALACGDA
                     TFQTYRFSAVLHTAEPSP"
     gene            complement(507..812)
                     /locus_tag="VGO32_01385"
     CDS             complement(507..812)
                     /locus_tag="VGO32_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV7199437.1"
                     /translation="MARDRATVTSGQRLTARVIGRVQGVGYRWWVVQRASALGLVGWV
                     RNADDERSVELVAEGDANALDAFEAELTAGPGGARVDAVEASRGSASGGWVRFEIRR"
     gene            complement(788..1897)
                     /locus_tag="VGO32_01390"
     CDS             complement(788..1897)
                     /locus_tag="VGO32_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199438.1"
                     /translation="MSTTAASPGFLAIGQPVAERLARRMLTEDAAPPTILVHGPAGSG
                     KGAFVDDLLATVLCEAIDPLGRPCNQCAGCWQARSRTHPDLVIASPARWREDRGTGES
                     IVGVARRWLAEVAGAPIASRWRVIVIEQADGANEQVQNALLKALEEPGDRQLFILVAD
                     DPRLLLPTIRSRVQSVRVGRVPSAELAEWLVSSAHVTDEQAVSLARLADGRVGRAMAF
                     ANAPDRVEWRRRTQHELLGLLERGRADRMASVRELLDEASQSALAIAPAVGDDETATR
                     TPTAVQRAAALAITDAWVDLARDLAVAAAGAPRLAASSELVPDLATAAQGRDARGWAD
                     AALAIERIRDALEENVSPRLALETAMLAWPEIAPR"
     gene            2114..2686
                     /locus_tag="VGO32_01395"
     CDS             2114..2686
                     /locus_tag="VGO32_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV7199439.1"
                     /translation="MSRTIEINEKDLDSRAQALAEAFGQAVEEAAERANAAWRETQPA
                     RHEAAKAIEEQGRQLGKMSRRLWRKEIRPRLQRFWNRRTVAIGAAGAAGAAVPAMLED
                     AAVELGLRPRRSHWGAFVAGIIIGGLTGVLVAMLTASKPGVETREELAARARDAAEAA
                     GEWIPVGISGANGNGGTGATPEATEVETEG"
     gene            complement(2683..4113)
                     /locus_tag="VGO32_01400"
     CDS             complement(2683..4113)
                     /locus_tag="VGO32_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV7199440.1"
                     /translation="MSKNVAVFVDVANLYYAARGQDVDVDYVALLKHATKGRDLIRAY
                     AYSGLDPENENQKKFLDFLGKNGYRVVAKDIRKFGDGRVKANLDIELVVDLFRLADRM
                     DVAVIVSGDGDFAPAIRALQDVGVRCEVISFKPNTSSDLFAVADEFHDAMKISAIGRQ
                     KETKGVGAPEMPAKEVNPDVGFREASPSVAAAALAAIRSDGDEPAGRGRGRGTRRPST
                     AAGRGGRGRTRSADAEPVASASATSEAAAPAAGAPHADNDSDTESGRRRRRRGGRGRG
                     RRGRRGDGPETAESTNGEPAEIRWDEIDSLDELDGTTAVSSPDQELGFAEADAATLAE
                     LGLSAQPVGVKDGDDPVGAPAAKPRTRTRSPSTKTAAKAATATTADDGGTTPRRSSST
                     KASTSAASKPAAKPKSSTAKKTADAAPAKTTRSRAPRAPKGDAAASKADAGRPAGDGS
                     DQGIWQRFTSGRTTKKGTTSEPPTEG"
     gene            4498..5802
                     /locus_tag="VGO32_01405"
     CDS             4498..5802
                     /locus_tag="VGO32_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7199441.1"
                     /translation="MEKFLKYSAGFVMLALVLAACTPSGSAAPSGSAAASETGAEEPA
                     AVCEALETGCFEVPAGEPLTVASALSITGDTAVLGIDTNVGIQVAQQERGQVMGRDVE
                     VLHEDAGCGDAATGQTAAEAIVANTAVLGVIGTTCSRTAIPAMPVLEEAGLIMISPSN
                     TAPGLTNPDHEQYAGPNYFRVAYNDKVQGAAVATYACDELGATTAATIHDGSPYAEQL
                     QQVFADEFNSICGGETVSQDAINVGDTDFRQLLTTISTANAGAAPDILFFPVFDPEGP
                     AIANQTQEIPALANTTLIGADGVKDQGFIDVAGDTAEAVGMLFSGPDLNFGTRYTDEF
                     VPAYEAIAGTPPTAPYHAHGYDSYNILMDAIEFATVGDDDAGTTYISKEGIRAYVAGL
                     TDYDGLTGTLTCDEFNDCGSPYVAIAQLEEGVFVNVFSTRPE"
     gene            5939..6985
                     /locus_tag="VGO32_01410"
     CDS             5939..6985
                     /locus_tag="VGO32_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7199442.1"
                     /translation="MRRLARISLVDVLLWILGGGIVLFVIVGSWLTLQSTHLSFEDWR
                     SLVIFGVAQGSVYALIALGYTLVYGVLLMINFAHGDVFMFGAMTSFFVADALAEAGFL
                     NANPILGYALVLAVATLSSMTVAVLLERIAYRPLRRAPRLVPLITAIGASLFISNSVR
                     GLYGEQVKAWPRIDALEGTIDIIGIPFLKTQAVVIVAAIVLMSFLVWFVNRTKTGRAM
                     RAVSENKDTAALMGIDVDRTIVITFATGGILAGAAGVLYGIAFYQVHWFMGFLPGIKA
                     FTAAVLGGIGNIVGAMLGGLTLGVLESIAPTLLLDGFGVPTPNQIKDVVAFTVLVLVL
                     IFRPYGILGRPEGK"
     gene            6986..>7480
                     /locus_tag="VGO32_01415"
     CDS             6986..>7480
                     /locus_tag="VGO32_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199443.1"
                     /translation="MLDRASFRRALTAGLTAGVAMLYLCAVGLVEAFIEREVVTGYVT
                     LGRVMLFAPPLLVGYWIGGKVTGGSRQVLSGLVAGLAGGVLFAMGFLIATALSPGIRE
                     VLLRITPALLSFIAFGLDPLPGAVLNVAVAAALGLIGAGSHALPARVRRPMAAGVGAV
                     VLLSM"
BASE COUNT         1087 a         2596 c         2567 g         1230 t
ORIGIN      
        1 cacctcgccc atcccaacga gattccggcc acctaccgtg acaccgatgc ccccctcgtc
       61 acccaggtct ccgactacgt gtccggcatg accgaccggt ttgcccaggc ggcctccgac
      121 cgcttgttcg gggcggggcc atcgcgatca gggtgagggt tcggcggtgt ggaggacggc
      181 gctgaaccga taggtctgga acgtcgcgtc accgcaggcc agcgcgatct cgtggggctg
      241 gtggcccggg tccgccgacg gtgtactgcc ttccggtccg tagacctcgc gcgtgagggc
      301 acccgcaacc gtgaatccca gcagcacggg ctcgccatcc cccacgaccg gcacgttggt
      361 cggttccccc accgcgtcat agagccggtc gccccactgg atggtgggcg gacagccggc
      421 ccggccgatg gccaggttga gcgcgagcag gcccagaccg atgaacaccg ccgcggccag
      481 caggccacag ccgaggatcc gccagttcat cggcggatct cgaatcggac ccagccgccg
      541 ctcgccgatc cgcgactggc ctcgaccgcg tccacccggg caccgcccgg cccggctgtg
      601 agctccgcct cgaacgcgtc gagggcgttg gcgtcgcctt ccgcgaccag ctcgaccgag
      661 cgttcgtcat cggcgttccg gacccagcca acgagcccca gcgccgaggc ccgttggacg
      721 acccaccacc ggtagccgac tccctgcacg cggccgatca cccgagcggt caggcgctgg
      781 cccgacgtca ccgtggcgcg atctcgggcc acgccagcat cgcggtctcc agcgcgaggc
      841 gtggactgac gttctcctcg agcgcgtccc ggatccgctc gatcgcgagg gccgcatcgg
      901 cccagccccg cgcgtctctc ccctgcgcgg cggtagcgag atccggcacg agctcggagg
      961 acgccgccag ccgaggcgcg ccggcggccg ccactgccag gtcgcgggcc aggtcgaccc
     1021 acgcgtcggt gatcgcgagc gcggccgcgc gctggacggc ggtcggggtt cgcgttgccg
     1081 tctcgtcgtc cccgactgcg ggcgcgatcg cgagcgcgct ttgggaggcc tcatcgagca
     1141 gctcacggac ggaggccatc cggtcggcgc ggccgcgctc caggaggccg agaagctcgt
     1201 gctgtgttcg gcgccgccac tcaacccggt ccggcgcgtt cgcaaaggcc atggcacggc
     1261 ccacccggcc gtccgcgagg cgggccagcg agacggcctg ctcgtcggtc acgtgggccg
     1321 agctcacgag ccactcggcc agctcggcgc ttggcacacg gccaacgcgg actgactgga
     1381 cgcgggaacg gatggtgggc aggagcaacc ggggatcgtc ggcgacgagg atgaacagct
     1441 gccgatcgcc gggctcctcg agcgccttca gcagcgcgtt ctgcacctgc tcgttggcgc
     1501 cgtccgcctg ctcgatcacg atgacccgcc agcggctggc gatcggcgca ccggcaacct
     1561 cggccagcca acggcgggcg acgccgacga tgctctcccc ggtcccgcgg tcctcgcgcc
     1621 agcgcgctgg cgaggcaatc accaggtcgg gatgggtccg cgaccgcgcc tgccagcatc
     1681 ccgcgcactg gttgcatggt cggccgagag ggtcgatcgc ctcacacagg acggtggcaa
     1741 ggagatcgtc cacgaatgcg cccttgcccg agccggcagg cccgtggacc aggatggtgg
     1801 gcggtgcggc gtcctcggtg agcatccgtc gagcgaggcg ttcggcgacc ggctgaccga
     1861 tggccagaaa tccgggcgag gccgccgtcg tgctcacggc ggcgagtata ggggccgtaa
     1921 gccgatggtc aggaacggct ggcaggatcg ggggacatgc ctcgggggtc gcccgggcac
     1981 ggcccgggcg accggttggc aaccaggttc gccgtgcgga acagacggcc cccggggcgc
     2041 cagatggcga aatcgcacct gcgatcggtc ggtgccatac tggtcctcaa tacaacgcag
     2101 ctcgagggcg gacatgagca ggacgatcga aatcaacgag aaggatctgg actcgcgcgc
     2161 gcaggcgctt gccgaggcat tcgggcaggc tgtcgaagag gctgcggagc gcgcgaacgc
     2221 cgcctggcgg gagacccagc cggcgcgcca cgaagccgca aaggccatcg aagagcaggg
     2281 tcgccagctc ggaaagatgt cgcgccgcct gtggcggaaa gaaattcgcc cccgcctcca
     2341 gcgcttctgg aatcgacgga cggtggcgat cggcgcagcc ggggcagccg gcgcagccgt
     2401 tccggcaatg ctcgaggatg ccgcggtcga gctcggcctc cggcctcgaa ggagccattg
     2461 gggcgcgttc gtggccggga tcatcatcgg cggcctgacc ggcgtgctgg tggcgatgct
     2521 gaccgcctcc aagccggggg tcgagactcg cgaggagctc gccgcccggg ctcgcgacgc
     2581 ggccgaagcc gcgggtgagt ggatcccggt tgggatctcg ggtgcgaacg gcaacggcgg
     2641 gaccggtgcc acgcccgagg cgaccgaggt cgagaccgaa ggctagccct cggtgggggg
     2701 ttcggaggtc gtacccttct tggtggtccg tcctgacgtg aagcgctgcc agatcccctg
     2761 atctgacccg tccccggcgg gacgccctgc gtccgccttg ctcgccgccg catcgccctt
     2821 cggggctcgt ggagcccgag atcgggtggt ctttgccggg gcagcatccg ccgtcttctt
     2881 ggccgtcgag gacttcggct tggccgcagg cttggacgcc gcgctggtcg aagccttcgt
     2941 gctcgaggag cgtcgcgggg tcgtcccgcc gtcatcggcg gtcgttgcgg ttgccgcctt
     3001 cgcggcggtc ttggtcgagg gggaccgagt ccgcgtccgg ggcttggctg ctggcgcacc
     3061 gaccgggtca tccccgtcct tcactccgac aggctgcgca ctcaggccca gctcggcgag
     3121 cgtggccgcg tcggcctcgg caaagcccag ctcctggtcg gggctcgaca ccgctgtcgt
     3181 gccatccagc tcgtccaggc tgtcgatctc gtcccaccga atctcggcag gctcgccatt
     3241 cgtggattcc gcggtctcgg gaccgtcgcc acgccggcct cgccggccgc gtccacgtcc
     3301 tccgcgtcgt cggcggcggc ggccgctctc cgtgtccgaa tcgttgtcgg catgcggcgc
     3361 gccggccgcc ggtgcggcgg cttcgctcgt ggcgctggcg ctcgcaaccg gttccgcgtc
     3421 tgcggatcgt gttcgtcccc gtcccccacg gcctgcggcc gtgcttgggc ggcgggtccc
     3481 ccggccgcga ccccggccgg cgggctcgtc gccatcagat cgaatggccg ccagcgcggc
     3541 cgcggcgacc gacggcgatg cctcgcggaa gccgacgtcg ggattgacct ccttggccgg
     3601 catctccggc gcaccgacgc ccttcgtttc cttctgacgg ccgatggcgc tgatcttcat
     3661 cgcgtcgtgg aactcgtcgg ccacggcgaa gaggtccgag ctggtgttgg gcttgaagct
     3721 gatcacctcg caccgaacgc cgacgtcctg aagggcgcgg atggcagggg cgaagtcgcc
     3781 gtcccctgac acgatgaccg cgacgtccat gcggtccgcc agccggaaaa ggtcgaccac
     3841 cagctcgatg tcgaggttgg ccttgacccg tccgtcgccg aacttgcgga tgtccttggc
     3901 caccactcgg tagccgttct tgccgaggaa gtccaggaac ttcttctggt tttcgttctc
     3961 ggggtcgagc cccgagtatg cgtaggcgcg aatcaggtcg cgccccttgg tggcgtgctt
     4021 cagcagcgcc acatagtcaa cgtccacgtc ctggccgcgg gcggcgtagt aaaggttggc
     4081 aacgtcaacg aagacggcca cgttcttgct cacgtgagca tttctccttg tgtgtgatga
     4141 gggtggcccc atggcgggat cccggctgcc ttcgcgctct gaggggtccc gagaggggac
     4201 tcatcggcgc gtctccggca gcgcccgccg cttcgcgaac cgttgagggt tccgcggcgg
     4261 cggcgggatc ggtgatctcc acctgggagg ccgagaggaa cccagcatac cacgcgccac
     4321 gggtggtccc gccaaagggg caccgggaat ggccgttgaa ccacctcggg ccaccggggc
     4381 tacccatcgg tgatcgagcc atcatcttgc gcacccggag gggcgcccgt atgatgcccg
     4441 gcacacttcc gggtggaacg ctgcgcgttt ccctggattc atttggagga ggattggatg
     4501 gagaagtttc tcaaatacag cgctggcttt gtgatgctgg cgctggttct ggcggcgtgt
     4561 accccgagcg gcagcgccgc tcccagcggg tccgcagccg ccagcgagac tggtgcggaa
     4621 gagcccgccg cggtgtgcga agccctcgag acaggatgct tcgaagtacc ggccggcgag
     4681 cccctgaccg tcgccagcgc cctctcgatc acgggtgaca cggctgtgct gggcatcgac
     4741 accaatgtcg gtatccaggt tgcgcagcaa gagcgcggcc aggtcatggg tcgcgacgtg
     4801 gaggttctcc acgaagacgc gggctgtggt gacgccgcta ccggccagac ggctgctgag
     4861 gcaatcgtgg ccaacaccgc cgttctcggc gtcattggta cgacgtgctc gcgaaccgcg
     4921 atcccggcca tgccggtgct cgaggaagcg ggcctgatca tgatctcgcc gtcgaacacg
     4981 gcgcccggtc tgaccaaccc ggatcacgag cagtacgctg gcccgaacta cttccgggtc
     5041 gcctacaacg acaaggtcca gggcgcagcc gttgccacgt acgcgtgcga cgagctgggg
     5101 gcgacgaccg cggccaccat ccacgatggc agcccgtacg ccgagcagct gcagcaggtg
     5161 tttgctgacg agttcaacag catctgcggc ggcgagaccg tctcgcagga cgcgatcaac
     5221 gtgggagaca cggacttccg tcagctcctg accacgatct cgaccgcgaa tgcaggcgcg
     5281 gcgcctgaca tcctgttctt cccggtgttc gacccggagg gaccggccat cgccaaccag
     5341 acgcaggaga ttcctgctct tgccaacacg accctgatcg gcgctgacgg cgtcaaggac
     5401 cagggcttca tcgacgtggc cggcgacacc gccgaggccg tcggcatgct cttctccggt
     5461 ccggacctga acttcgggac ccggtacacg gacgagttcg tcccggccta cgaggcgatc
     5521 gcaggcacgc cgccgaccgc tccgtaccac gcgcatggtt acgactcgta caacatcctc
     5581 atggatgcga tcgagttcgc gaccgtcggt gacgacgacg cgggcacgac gtacatcagc
     5641 aaggaaggga tccgggccta cgtggccggc ttgaccgact acgacggact caccggtacc
     5701 ctcacctgcg atgagttcaa cgactgtggg tcgccgtacg tggcgatcgc tcagctcgag
     5761 gaaggcgtct tcgtcaacgt gttcagcacc cgaccggagt aaccggctcg gtagcgaacc
     5821 aacgcttcgg ggcgggtggg ccgacaacgg ctcacccgcc atctgtatag tccgcgggac
     5881 tgtctgattc ccggccccgc acgcccattc ccactccctt gcccggagag agaaactggt
     5941 gagacgcctc gctcggatct ccctggtcga cgttctcctg tggatcctcg gtggcggcat
     6001 cgtcctgttc gtcatcgtcg gcagctggct gaccctccag tcgacccacc tgtcgttcga
     6061 agactggcgc tcgctggtca tctttggcgt cgcccaggga agtgtttacg cgctcatcgc
     6121 gctcgggtac accctggtgt acggcgtcct gctgatgatc aacttcgccc atggcgacgt
     6181 cttcatgttc ggggccatga cctcgttctt tgtggccgat gccctggccg aagccggctt
     6241 cctgaatgcc aacccgattc tcgggtacgc cctggtcctc gccgtcgcca ccttgagctc
     6301 gatgacggtc gccgtgcttc tcgagcgcat cgcctatcgc ccgctgcgac gcgctccgag
     6361 actggtgccg ctcatcaccg ccatcggggc ctcgctgttc atcagcaaca gcgtgcgtgg
     6421 cttatacggc gagcaggtca aggcctggcc gcggatcgat gccctcgagg gcacgatcga
     6481 catcatcggc attcccttcc tgaagaccca ggccgtggtc atcgtggccg cgatcgtgct
     6541 gatgtcgttc cttgtctggt tcgtgaaccg gaccaagacc ggacgagcga tgcgcgccgt
     6601 gagcgagaac aaggacaccg cggcactgat ggggatcgac gtcgaccgca cgatcgtgat
     6661 cacgttcgcg accggcggga tcctggccgg agccgccggc gtgctgtacg gaatcgcctt
     6721 ctaccaggtc cactggttca tgggattcct gcccgggatc aaggcgttca ccgcggccgt
     6781 cctgggcggc atcggcaata tcgtgggtgc catgctcggc ggcctgaccc tgggcgtcct
     6841 ggagtccatc gcccccaccc tgctgctgga cggcttcggg gtgccgacgc ccaaccagat
     6901 caaggacgtc gtcgcattca ccgtcctcgt gctggtgctc atcttccggc cgtatggcat
     6961 cctcggccga ccggagggca agtagatgct ggatcgcgcg tcgttccgac gagccttgac
     7021 cgcgggcctg accgccggcg tggcgatgct ctacctgtgc gcggtgggcc tggtcgaggc
     7081 gttcatcgaa cgcgaggtcg tcacgggcta cgtgaccctg ggccgggtga tgctgttcgc
     7141 gcccccgctg ctggtcgggt actggatcgg tgggaaggtg accggcggct cgcggcaggt
     7201 tctctccggg ctggtcgccg ggctcgcggg cggcgtgctg ttcgcgatgg gcttcctcat
     7261 cgccacagcg ctgtcgccgg gaattcggga agtcctcctg cggatcacgc cggccctgct
     7321 ctcgttcatc gccttcgggc ttgaccccct ccccggtgcc gtccttaacg tggcggtcgc
     7381 ggccgccctc ggcctgatcg gcgcggggag ccacgcgctc cccgctcgcg ttcggcggcc
     7441 gatggccgct ggcgtcggcg cggtcgtcct gctgagcatg
//
LOCUS       DASYGU010000053         8419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1550891, whole genome shotgun sequence.
ACCESSION   DASYGU010000053 DASYGU010000000
VERSION     DASYGU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550891"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGO32_01420"
     CDS             <1..222
                     /locus_tag="VGO32_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199444.1"
                     /translation="AVFHEFVSLSIQHLVNEIGGRMLERWPQLAEVSFESQNRTWDPG
                     ETNPDDPRVKTYADPRPPIGRIGLVMRRS"
     gene            complement(244..2985)
                     /locus_tag="VGO32_01425"
     CDS             complement(244..2985)
                     /locus_tag="VGO32_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEV7199445.1"
                     /translation="MESTIVDVAEQRRLNDARELQIPWKKWGPYLSERQWGTVREDYS
                     EDGSAWDYFGHDQARSRAYRWGEDGLGGLSDDRQRLCFALALWNERDPFLKERLFGLT
                     NGQGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQREYPYQDLLETNRQRTREDPEYEL
                     LDTGAFDEDRYFDVFLEYAKESPEATLIRITVHNRGPESATLRVMPTLWFRNTWSWDP
                     DPPRPVLRQAEPGVIEATHHELGTMRLTCEGSPELLFTENESNSARLWGQVNATSYVK
                     DAFHEFVVAGRADAVNPALEGTKAAAHYALEIPAGGSQTVQLRLAPGSEDAALAGFDE
                     TFNQRIADADEFYERIAPAALTGDERLIHRQALAGMLWSKQYYYFDVDRWLQDHGSHP
                     IIESTRAGIRNTEWFHMLNADVISMPDKWEYPWYAAWDLAFHTLSLALVDFDFAKEQL
                     LLMLRSLYAHPNGQIPAYEWNFGDVNPPVHAWATLSLYKTERALGREDRQFLQRAFQG
                     LMMNFNWWVNRKDPDGRNVFAGGFLGLDNIGVFDRSAQLPTGGTLEQADGTAWMAFYC
                     QNMLEIALILSEHDPMYEEVAYRYAEHFIWISYAMDRMGDQQDEMWDEEDGFFYDLLR
                     LPEGPAQRLKVRSLVGLLPLCASTVFEGDALERYPKLMELIALFRKRHPNLLSYVAPT
                     SEGFVGYKGRRLLSILNKRKLERVLSYMLDENEFLGPYGIRSLSRYHLDHPFVFPVGE
                     LEFSVKYLPGESDSGMFGGNSNWRGPVWMPVNALLLRALLNLYSFYGDEFTVECPTGS
                     GHHMTLFEVAQEISRRLASIFLREPSVNPGEIGRRPVNGGSATFQRDPNWQDLILFYE
                     YFDGDTGAGLGASHQTGWTGLIARVLDMFARVEAKDLELDLGHVSARLVIEQVGGER"
     gene            complement(3063..3500)
                     /locus_tag="VGO32_01430"
     CDS             complement(3063..3500)
                     /locus_tag="VGO32_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV7199446.1"
                     /translation="MTLSELATIEKVLRETETWAVVGCSSNPNRPSHGVARTLIRSGY
                     RMIPVNPRETEVHGQRAYADLFAAQGDAVAAGHPIEVVDIFRNSALAGIHVDEAIEIG
                     ARAVWMQLDVIDEEAAERAQAAGLWVVMDRCPAIELPRLSLGA"
     gene            complement(3497..4072)
                     /gene="ruvA"
                     /locus_tag="VGO32_01435"
     CDS             complement(3497..4072)
                     /gene="ruvA"
                     /locus_tag="VGO32_01435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV7199447.1"
                     /translation="MIGSLRGPVTHIGLDYVIIELGGVGYRVVVAPALIGRLRTGSEA
                     SLFIHHLVREDQQALFGFAKPEELAFFELLMTVAGVGPRLALAITSAHPVTRLQMAIV
                     TDDLDVLTSVSGVGLKTAQRIVLELREKIHAAGIAVGPGGAADSDVVAALESLGYTAS
                     EARRAAGSVAAIEGGLDERIRAALQELARSR"
     gene            complement(4069..4545)
                     /gene="ruvC"
                     /locus_tag="VGO32_01440"
     CDS             complement(4069..4545)
                     /gene="ruvC"
                     /locus_tag="VGO32_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV7199448.1"
                     /translation="MIALGIDPGTAACGFGIVASDGSNLRFVDAGTVRTDPALTDSAR
                     LAELEAALDQLVAQHQPDRVAVERLFFQRNVQTAMAVGQARGVALLVAARHGLPVTEP
                     TPNEVKLAVCGNGAADKQQVAVMVGRLLGVHLNGAGDDATDALAIAIGALAPASLA"
     gene            complement(4542..5306)
                     /locus_tag="VGO32_01445"
     CDS             complement(4542..5306)
                     /locus_tag="VGO32_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7199449.1"
                     /translation="MSGHSKWSQIKRQKGANDAKRGALFTKLTREIITAARQGGPDTE
                     ANYRLRMAVDKARASSMPADNIKRAIERATGSGADAEQYEEITYEGLGPENVAVIVAT
                     MTDNKNRTAAEIRAVFTRAGGSFAPVSWQFEHRGLLSVPLNGNDPDEVSLAAIDAGAA
                     DVGTPDGGFILVVTDPAGLEGVRTALTDAGFPPETAEVSMEPTTRVEIGDEKGARHVL
                     DFVERLEDLEDVQTVYANFDIPDALMEQLEASVVGG"
     gene            5386..6435
                     /locus_tag="VGO32_01450"
     CDS             5386..6435
                     /locus_tag="VGO32_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEV7199450.1"
                     /translation="MDSVGTPALLAILAGGAAATWVAGIFLSRATDGIDDHFDLGQAL
                     GGMLLLGIAGTLPEIAITVSAALSGHLDLAVGNLLGGIAMQTLVLVILDATSRNRRPL
                     SNLSDSLEPLHEAALVIGVVGIAVMGTQLAPSATIGPMSPASLAIVIVWLLGIFALNR
                     NRRSPRWQSADALAAPAPAPVPDTPKASVGRSRPIGGTLLMFGIASAVTLVAGVLLEQ
                     SGNALADDFGINGAIFGATVLAAITALPEISTGLAAVRMGQVGLAMGDIFGGNAIQVT
                     LFLLADLLAGQAVLTTASVESIWLACLGIVVTAIYLNGLITRPARKVLGLGPDSLAVA
                     VAYLFGVIGLLYLTT"
     gene            complement(6437..>8419)
                     /locus_tag="VGO32_01455"
     CDS             complement(6437..>8419)
                     /locus_tag="VGO32_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7199451.1"
                     /translation="GLRRRGWSGTASAAAVTGAAVLVGGLLLLLLALAFAPYIPTLVS
                     AFQTGVESVRQQVAASGSPELAVQIAAAAANFQAWLSDNFASLVGSVANAFTVGLLAL
                     FLTFFLLQDGEKGWGRAIEVTDGWRRERIDDAGHTALTRVGGYLRGTAVLSAVVAVTD
                     LVYLTVLGVPLAAPLAILAFVGGFVPYIGGLIATGVILLAAWGAVGPQTAIVLLVLIA
                     VTRGIVSNVLRPVVYGSRVGLHPGIILLVLPAGAAVAGIVGVFAAVPVAVFVASFTAS
                     IIAALEPEVAPDRNPIVPHWLDRVAQWGWRLLAILVVGIGMILILGTVPLVVAPLLLA
                     GILAASVVPLVAALRRRGWSNQRAAMVVTGGSVLAIVAIMVVAVVALGPGLSDAISGA
                     EEGAADAGDATDGVLGWLETASTQVGTSIAGIVTGVLSGVSSLVVVLVLASLLTFYVL
                     RDGRRGWDRITQPLGDWQRTELETTMTDSIGVLGGYMGGTAVISAVGAVSQLLIMLIL
                     GLPFAVPVAVLSFFACFIPYVGGFVTTGLAFLIAVAFGDTTTILIMGIYTLVFNIVQG
                     NVVTPLVYRRAVHLHPAIILLAIPAGGALAGILGMFMAVPIIALIATARLPIMRLLSG
                     EPATTDSAEGVIATGSPPATAPAKSGSAPEPAAEAG"
BASE COUNT         1423 a         2878 c         2775 g         1343 t
ORIGIN      
        1 gcggtcttcc acgagttcgt gagcctctcg atccagcacc tggtcaacga gatcggcgga
       61 cgcatgctcg aacggtggcc gcagctggcc gaggtcagct tcgagtcgca gaaccggacc
      121 tgggacccgg gcgagacgaa cccggacgac ccgagggtga agacctacgc cgacccgcga
      181 ccgcccatcg gccgcatcgg cctcgtcatg cgtcggagct gaacaggagc gccgcgccga
      241 cccttagcgc tcaccaccca cctgctcgat caccagccga gcggatacgt gaccaaggtc
      301 gagctccagg tccttggcct cgactcgcgc gaacatgtcg agcacgcgag cgatcagccc
      361 ggtccagccc gtctggtggc tcgcgccgag gcccgccccg gtgtccccgt cgaagtactc
      421 gtagaacagg atcaggtcct gccagttggg gtcgcgctgg aacgttgccg acccgccgtt
      481 gacaggtcgc cgtccgatct cgccggggtt gaccgacggt tcccgcagga agatgctcgc
      541 cagccggcga ctgatctcct gagccacctc gaagagcgtc atgtgatgcc cggatccggt
      601 cgggcattca accgtgaact cgtcaccgta gaagctgtag agattcagca gggccctgag
      661 cagcagggca ttgaccggca tccagacagg cccccgccag ttggagttcc caccgaacat
      721 cccgctgtcc gattcacccg gaaggtactt caccgagaat tcgagctcgc cgaccgggaa
      781 tacaaacgga tggtcgaggt ggtaccgaga cagggatcga atgccgtagg gaccgagaaa
      841 ctcgttctca tcgagcatgt aggaaaggac ccgctcgagc tttcgcttgt tgaggatcga
      901 aagcagacgg cgtcctttgt aaccgacaaa cccttcgctc gtgggtgcca cgtacgagag
      961 cagatttgga tgtcgcttcc tgaacagggc gatgagctcc atgagttttg gataccgctc
     1021 caacgcgtcg ccctcgaaca cggtggacgc gcacagaggc aggagcccca ccagggaccg
     1081 gactttcaac cgctgcgccg gcccttctgg tagccgtaac aaatcgtaga aaaagccgtc
     1141 ctcctcatcc cacatctcgt cctgctgatc ccccatccgg tccatggcat acgagatcca
     1201 gatgaaatgt tcggcgtacc gatacgccac ttcctcgtac atcggatcgt gctcggagag
     1261 gatcagcgcg atctcgagca tgttctggca gtagaaggcc atccatgccg tcccgtccgc
     1321 ctgctcgagg gtgccacccg tcgggagctg ggcgctccgg tcgaagaccc cgatgttgtc
     1381 gaggccgaga aagccgcccg caaagacgtt ccggccatct gggtccttgc ggttgaccca
     1441 ccagttgaaa ttcatcatca ggccctggaa cgcccgctgc aggaattgcc gatcctcacg
     1501 gccaagcgcg cgctccgtct tgtacagcga cagcgtcgcc cacgcatgga ccggcggatt
     1561 gacatcgccg aagttccatt cgtaggcggg aatctgcccg ttcgggtggg cgtacaggct
     1621 gcggagcatg agcaggagct gctccttggc gaaatcgaaa tcgacaagcg caagggacag
     1681 ggtgtggaag gcgaggtccc aggccgcgta ccacgggtac tcccacttgt ccggcatcga
     1741 gatcacgtcc gcgttcaaca tgtggaacca ctcggtattc cggatgcccg cccgggtcga
     1801 ttcgatgatc ggatggctcc cgtggtcctg cagccagcgg tccacgtcga agtagtagta
     1861 ctgcttggac cagagcatcc cggccagtgc ctggcgatga atgagccgct catcgccggt
     1921 cagggcggcg ggtgcaatcc gctcgtagaa ctcgtcggca tcggcgattc gctggttgaa
     1981 cgtctcatca aatccggcga gagcggcgtc ttcgctgcct ggtgccagac ggagctgcac
     2041 ggtctggctt ccaccggcgg gtatttccag cgcgtagtga gcggcggcct tggttccctc
     2101 cagagcggga ttgaccgcgt ctgcccggcc cgcgaccacg aactcatgga atgcgtcctt
     2161 gacatacgac gtggcattga cctggcccca caggcgggcg ctgttgctct cgttctcggt
     2221 gaacagcagt tccggcgaac cctcgcaggt caggcgcatg gtgccgagct cgtggtgagt
     2281 ggcctcgatg acgccgggct cggcctggcg gagcacgggc cgtggcggat ccggatccca
     2341 tgaccacgtg ttgcggaacc acagcgtggg catgacacga agggtggccg attccggtcc
     2401 acggttgtgc acggtgatgc ggatgagcgt ggcttccgga ctctccttgg cgtattcgag
     2461 aaaaacgtcg aagtatcggt cctcgtcgaa cgcgccggta tccagcagct cgtactccgg
     2521 gtcttcccgc gttcgctgcc ggttggtctc cagcaggtcc tgatagggat actcgcgctg
     2581 cggatacttg tagagatatt tcatgtagga gtgcgtgggt gtgctatcga ggtagaagta
     2641 gtactccttc acgtcctcgc catggtttcc ctggccattg gtcagcccga acaatcgctc
     2701 cttcaggaag gggtcccgct cattccacag ggcgagcgcg aagcacagcc gctgccggtc
     2761 gtcgctgaga ccgccgagcc cgtcctcgcc ccagcggtag gcccgtgagc gggcctggtc
     2821 gtgcccgaaa tagtcccacg cgctgccgtc ctcgctgtag tcctcgcgga ccgtgcccca
     2881 ttggcgctcg ctgagatacg gtccccactt cttccacggg atctgcagct cgcgcgcgtc
     2941 attcagccgc ctctgctcgg cgacgtcaac aattgttgat tccactgatg gcctccgcct
     3001 cctgttgcgc gccaacgttc acagtccggc aataggccgg ggttaagcgg ctgccgagga
     3061 cgtcatgcgc cgagactcag ccgcgggagc tcgattgccg ggcagcggtc catcaccacc
     3121 cacaggcccg ccgcttgggc gcgctcggcc gcctcctcgt cgatgacgtc caactgcatc
     3181 cagaccgccc tggcgcctat ctcgatcgcc tcgtcgacgt ggatcccggc cagggccgag
     3241 ttgcggaaga tgtcgaccac ctcgatcggg tggccggccg cgaccgcgtc accctgggcc
     3301 gcgaacaggt ccgcgtacgc ccgctggccg tgcacttcgg tttcgcgcgg gttgaccggg
     3361 atcatccggt agccgctccg aatcagcgtt cgcgcaacac cgtgactggg tcggttgggg
     3421 ttcgacgagc agccgaccac cgcccaggtc tcggtctcgc gcaggacctt ctcgatcgtc
     3481 gccagctcgg agagggtcat cgggagcgtg cgagctcctg gagcgcggcc cggatccgct
     3541 cgtccaaccc gccctcgata gcggccacgc tgcccgccgc ccgacgggcc tccgaggcgg
     3601 tgtagcccag cgattcgagc gccgcgacga cgtccgagtc ggccgcgccg cccggtccga
     3661 ccgcgatccc cgcggcgtgg atcttctccc gcagctcgag cacgatccgt tgcgcggtct
     3721 tcaggccgac accggagacg gaggtcagga cgtccagatc atcggtgacg atggccatct
     3781 gcagccgggt gacgggatga gcgctggtga tggcaagggc cagccgcggg cctacgccgg
     3841 ccacggtcat cagcagctcg aagaacgcga gctcctcggg cttggcgaac ccgaacagtg
     3901 cctgctggtc ctcgcggacc aggtggtgaa tgaacaggct ggcctccgag cccgtccgca
     3961 gccggccgat cagtgcgggt gccacgacca cccggtagcc gacgccgccc agctcgatga
     4021 tcacgtaatc cagcccgatg tgggtcaccg ggccgcggag cgagccgatc acgcgaggct
     4081 cgccggagca agggcgccga tggcgatcgc cagcgcatcg gtcgcgtcat cgcccgcacc
     4141 gttgagatgc acccccagca gtcgcccaac catgaccgcc acctgctgct tgtcggccgc
     4201 gccgttgccg cacaccgcga gcttcacctc gttgggcgtg ggctcggtga ccggcagccc
     4261 gtggcgggcg gccacgagca gggccacgcc tcgtgcctgc ccgacggcca tcgcggtctg
     4321 cacgttgcgc tggaagaaga ggcgctcgac cgccacccgg tccggctggt gttgggcgac
     4381 gagctggtcc agcgcggcct cgagctcggc caatcgcgcg gagtcggtca gcgccgggtc
     4441 ggtccggacc gtgccggcgt ccacgaaacg cagattggaa ccatccgacg ccacgatccc
     4501 gaacccgcag gcggccgttc cggggtcgat cccgagggcg atcatccgcc tacgaccgat
     4561 gcctcgagct gctccatcag cgcatcggga atgtcgaagt tggcgtacac cgtctgcacg
     4621 tcctcgaggt cctcgagccg ctcgacgaag tcgagcacgt ggcgtgctcc cttctcgtcg
     4681 ccgatctcga ccctggtcgt cggctccatg gacacctcgg ccgtctccgg cgggaagccg
     4741 gcatcggtca gggccgtccg cacgccctcg aggcctgccg gatcggtcac gaccaggatg
     4801 aacccgccgt ccggggtgcc cacgtcggcc gcgcccgcat cgatggcggc cagcgacacc
     4861 tcgtcggggt cgttcccgtt gagcggcacg gacagcaggc cgcggtgctc gaactgccac
     4921 gacaccgggg cgaacgagcc gccggcccgg gtgaagacgg cgcggatctc ggcggcggtc
     4981 cgattcttgt tgtcggtcat ggtggcgacg atgacggcca cgttctcggg gccgagtccc
     5041 tcgtacgtga tctcctcgta ctgctccgca tcggcgccgg agccggtcgc gcgctcgatc
     5101 gcgcgcttga tgttgtcggc gggcatcgag ctggcgcgcg ccttgtccac ggccatccgg
     5161 aggcggtagt tggcctcggt gtccgggcca ccctgccggg cggcggtgat gatctcgcgg
     5221 gtcagcttgg tgaacagcgc cccgcgcttg gcgtcattcg cgcctttctg gcgcttgatc
     5281 tgcgaccact tgctgtgtcc ggacatcgcc taagactacg acgcggaacc gagcggcgct
     5341 gcatgcggat gaccgacggc ttggtccgac aaatacaatt cggcgatgga ctcagtcggg
     5401 acgcccgcac tgcttgcgat ccttgccggg ggcgcggcgg caacgtgggt cgcgggcatc
     5461 tttctctcac gggcgaccga cggaatcgac gaccacttcg acctcggcca ggcgctgggc
     5521 gggatgctcc tgctcggcat cgccggaacg ctccccgaga tcgcgatcac ggtctccgcc
     5581 gcgctgagtg gccaccttga cctcgccgtc ggcaacctgc tcggcgggat cgcgatgcag
     5641 acgctggtgc tcgtgatcct cgacgccaca tcgagaaacc ggcgaccgct gtcgaatctg
     5701 tccgactccc tcgaaccgct gcacgaagcg gccctggtca tcggcgtggt gggcatcgcg
     5761 gtgatgggca cccagctcgc gccatcggcc acgatcggcc cgatgagccc cgcatcattg
     5821 gcgatcgtga tcgtctggct cctcggcatt ttcgccttga accgcaaccg ccggtcgcct
     5881 cggtggcaat ccgccgatgc gctcgccgcg ccagcgccgg ctcccgttcc cgacaccccg
     5941 aaggcttcgg ttggccgatc ccgaccgatc ggcggaacgc tcctgatgtt cggaatagct
     6001 tctgcggtga cgctcgtcgc gggcgtcctg ctggagcaga gcggcaacgc gctcgcggat
     6061 gacttcggca tcaacggcgc cattttcggg gcgacggtcc tggccgcgat caccgcgctg
     6121 ccggagatca gcaccggact cgccgcggtt cgcatgggcc aggtcggcct cgcgatggga
     6181 gacatcttcg ggggcaacgc catccaggta acgctgttcc tcctggccga cctcctggct
     6241 ggtcaggcgg tgctgacgac cgcgagcgtg gagtccatct ggctcgcatg cctgggcatc
     6301 gtcgtcaccg cgatctatct gaacggcctg atcacccggc ccgcgcgcaa ggtcctgggc
     6361 ctcggcccgg actcgctcgc tgtagccgtg gcctatctgt tcggggtgat cggcctgctg
     6421 tacctcacga cctaggctat ccggcttccg cggcgggctc tggcgcggaa cctgacttgg
     6481 ccggtgccgt ggcgggcgga ctgcccgttg cgatgacgcc ttcggccgag tccgtggtcg
     6541 ctggctctcc gctcagcagg cgcatgatcg ggagccgggc ggtggcaatc agggcaatga
     6601 tcgggacggc catgaacatg cccaggatcc cggccagggc tccgccggcc ggaatggcga
     6661 gcaggatgat cgccgggtgg aggtggacgg cccggcgata gacgagtggc gtcaccacgt
     6721 tgccctggac gatgttgaaa accagggtgt agatgcccat gatcaggatc gtggtcgtat
     6781 ccccgaacgc gaccgcgatg agaaacgcga gaccggtcgt cacgaatccg cccacgtacg
     6841 ggatgaagca ggcgaagaac gacaggacgg cgacgggcac cgcaaacggc aggcccagga
     6901 tgagcatgat caggagctgg gagaccgcgc cgacggccga gatgaccgcc gtgccgccca
     6961 tgtagccgcc gaggaccccg atcgagtccg tcatcgtggt ctcgagctcg gtgcgctgcc
     7021 agtcgccgag cggctgggtg atgcggtccc agccgcggcg gccgtcgcgg aggacataga
     7081 acgtgagcag gctggccagg acgagcacca cgaccaggga agacaccccg gacaggacgc
     7141 cggtcacgat cccggcgatc gatgttccga cctgcgtcga ggcggtctcg agccacccga
     7201 gaacgccatc ggtcgcgtcg cccgcgtcgg cagccccctc ctccgcgcca ctgatcgcgt
     7261 cgctcagtcc ggggccaaga gcaacgaccg cgacgaccat gatcgccacg atggcgagca
     7321 ccgacccgcc ggtcaccacc atcgccgccc gctggttgct ccagccgcga cgacggagcg
     7381 ccgcgacgag gggaaccacc gacgcggcca ggatccccgc caggaggagc ggcgccacga
     7441 ccagggggac ggtgcccagg atcaggatca tcccgatgcc gacgaccagg atcgcgagga
     7501 ggcgccatcc ccattgggcc acgcggtcca gccaatgcgg gacgatggga ttcctgtcgg
     7561 gtgccacctc gggctcgagg gcggcgatga tcgacgcggt gaacgaagcg acgaacacgg
     7621 ccaccggaac cgcggcgaac acgcccacga tgccggccac cgccgcgcct gcagggagga
     7681 cgagcaggat gatcccgggg tggagaccca cgcgcgaccc gtagaccacc ggtctgagca
     7741 cgttcgacac gatcccgcgc gtgacggcaa tgagcaccag gagaacgatg gctgtctgag
     7801 ggccgaccgc cccccacgcg gccagcagga tgacgccggt cgcgatcagg ccgccgatgt
     7861 agggcacgaa cccgccgacg aaggcgagga tcgcgagcgg ggctgccagc ggaacaccga
     7921 gcacggtcag gtacaccagg tcggtcaccg ccaccaccgc cgatagcacc gctgtgccgc
     7981 gcaggtatcc accgacccgg gtcagggccg tgtgtccagc atcatcgatc cgttcgcgac
     8041 gccagccatc ggtcacctcg atcgcgcggc cccatccctt ctcaccgtcc tggagcagga
     8101 agaaggtcag gaagagggcc agcaagccga ccgtgaacgc attcgcaacg ctgccgacca
     8161 gcgacgcaaa gttgtcgctg agccaggctt ggaagttcgc tgccgccgca gcaatctgca
     8221 ccgcgagctc gggcgaaccg gaggccgcca cctgttgccg gaccgactcg acgcccgtct
     8281 ggaaggcaga cacgagagtc ggaatgtacg gcgcgaacgc gagcgccagg aggaggagca
     8341 gcaggccccc gacgaggacc gcggcccccg tcaccgcggc cgcactcgcg gtccctgacc
     8401 agccacggcg acgcagacc
//
LOCUS       DASYGU010000054         6207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_573829, whole genome shotgun sequence.
ACCESSION   DASYGU010000054 DASYGU010000000
VERSION     DASYGU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573829"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..638
                     /locus_tag="VGO32_01460"
     CDS             216..638
                     /locus_tag="VGO32_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199452.1"
                     /translation="MTRPPAWRFAGIFIVLGLAVAACSPASDQPSAEQALCDALATFG
                     DSVQAIADTDPATDSVEDIQAAADAATEAWGGVQTAVAAVDEADDTAVDAAWSDLADQ
                     IGNFATDVPVADAVDQISGGVDEVQAAYDEMRDGLGCQ"
     gene            1395..3023
                     /locus_tag="VGO32_01465"
     CDS             1395..3023
                     /locus_tag="VGO32_01465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7199453.1"
                     /translation="MNSPSVAASARATGLPAPRRRWLRTVRIGLAAVVVAFLLAYAVG
                     QRGHTTWTRGTILAVEQDLVGAIWPLAQSFDSPRGYGYTGDNPYPHLIQGDFGGTAFY
                     PPGYPFLLWITARALGDPMRATQLINAVAGVLFILASYWLAVGWTGKRGLALLSAAFV
                     AGGAVFLTLAYAASTDLLAGAASTAALACTAWALRSPTRRRWVLLAGITLGLAFLLRR
                     QTAVLLAPLIILVVMSARGRPREAVIRAGMLVGVFGLLALPDLLIQNSLHGSPLYGAH
                     GKNIWNAVVNRYDWPAWHSVPNTISALSVIVSAPAAFVGNWFGNLLYYAERLWLGPAT
                     LLALPGAYLLWRRRWLGKMTAAILGSAAFVVIVASVSERGDRHALILVPTLAVPAAAA
                     LWYAVSRRWRLPVAAAIVILAASVGIAHTAVNITVDLRVPHLDEVVAALHEDGAQPDD
                     VLTSFNSAHYAFYAGIGFVPLSGEVYTWDDLRRFMDEHGVRYYVARGIGFHVRRLTES
                     LEPTAAPDWIDPIWVSQPDRDNGRIVLYRYDGGP"
     gene            complement(3036..4580)
                     /locus_tag="VGO32_01470"
     CDS             complement(3036..4580)
                     /locus_tag="VGO32_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199454.1"
                     /translation="MISIAPIAFPVLLLFGYLSALLMVVHALASHLPGRRVGPFLVVP
                     LVVAIGLTVVLVVLFAGESGRLLSRFAGGGLASGEFRLPPSATYIAIGAAFLIAVAVV
                     HFGASEIPGWAVRRVQRQSAATAEDSDGGATVADVLRAVLLGGAGVAIVGTSFVFGQT
                     AAATTRQGQGAAVVEEFPLDGGPTGLVITEDHASAYVTLGDGRIVRMELDQDGLPGTN
                     VEVVAEGLSFPRGPAIVDGELFVADLGTLACPDPFPQCWTPEPAEELDRINASSASVL
                     AFEIQTDGSLGEPREVLTDLPVVNTEHAPNSITLGPDGYLYMPIAGPDRLPLDLALAE
                     DITHPNADLLGTIVRFLPAGGDVEVIASGIRNIYSLTFDPDGHLYGVDNDGQTGRGWR
                     HEQLIEVVDGADYGYPAHGTFDGTSTQEPLWILDTIGSTGTDWSAVDGATGVLVGSAS
                     RLDLVRLDFDGDGPYVADERSAVTMLQGLNGFVTGIEAVGDGRYLMSVLRFEGQNALL
                     VVDIGE"
     gene            4725..4799
                     /locus_tag="VGO32_01475"
     tRNA            4725..4799
                     /locus_tag="VGO32_01475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4757..4759,aa:Met,seq:cat)
     gene            4820..5758
                     /gene="tilS"
                     /locus_tag="VGO32_01480"
     CDS             4820..5758
                     /gene="tilS"
                     /locus_tag="VGO32_01480"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV7199455.1"
                     /translation="MPSRAVAEAATRLPIPDGATMVLAVSGGPDSMALLHGARAVAPS
                     HSWQLVVAHLDHGLRPDSADDATFVTDAADELGLPWRLRRTDVAAAAAADRLGIEGAG
                     RVARYAFLDEVADGLGSATLVVTAHSADDQAETVLMNLARGSGLHGLRGIPARRGRVI
                     RPLLATRRRALRTALDEAGIAYRLDPTNADPAHARARVRADLVPAMESLNPAAVDAVV
                     RFAALAADDDALLDALAADELRRRGTDGALDWHDPPRPALGRRVLRLAIGDPAPSADR
                     IEAVLEAAAGPRGGITIELGRGRTAEIRGRRIVIGL"
     gene            5873..>6207
                     /locus_tag="VGO32_01485"
     CDS             5873..>6207
                     /locus_tag="VGO32_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199456.1"
                     /translation="MNSRLVRSSILIVGALFAMALLWTYLMDTQEGPAYTYSDLLTDA
                     AAGNVTGVTQDGTTLTVSFEGSDTTKTAIVPSDQVNVFAEVCAASGATGLADCDITYE
                     AVAESATGQ"
BASE COUNT          925 a         2174 c         2081 g         1027 t
ORIGIN      
        1 caccattccc cacaggaaat ccccgaacga cattgagtac tcctcacatt gggccggttg
       61 ggcggcccgg cccgtagttg gctgattccg agtgtagcag ccgatctcgc gtgcgcgaag
      121 agggctctcg gtcgccgcgg agcccctcgt ggcgctgcct gctacgatcc taggctccag
      181 ggattgaatt cccatacatc aggagggcac accccgtgac acgaccacct gcctggcgat
      241 tcgccggcat cttcatcgtc cttgggctcg cggtcgccgc atgctccccg gcctcggacc
      301 agcccagcgc ggagcaggcg ctgtgcgatg cactggcaac cttcggcgac agcgtccagg
      361 ccattgccga cacggatccg gcgaccgact ccgtcgagga catccaggcg gccgcggacg
      421 cagccaccga ggcgtggggc ggagtccaga ctgccgtcgc cgcagttgac gaggccgatg
      481 acaccgcggt cgacgccgcg tggagtgacc ttgccgatca gatcggcaac ttcgccaccg
      541 acgtccctgt ggcggatgcg gtcgaccaga tcagcggcgg cgttgacgag gtccaggctg
      601 cctacgacga gatgcgcgac ggccttggct gccagtagtc ggatctcgcg ggagccaacc
      661 cgcagctgaa ccggaatggc cgggctgaac agcccggcca ttcatttgag gtaggaccga
      721 cgcgctgaat ggtccgaacg gcgcccagcg tgcctgcgac gcgactccaa gttgaccgtt
      781 gcttctggtc aacacgagtc ctacgatgct gctcctcgac gtgcttcgat tgacccgcca
      841 tcggagggtt gcccagtggt cccgacaccc gctgcgtgga ggcagcatgg cgcgctggcc
      901 gggttcaccg cggcgctcct cctcgtctcc gcggtgctgg gtatcgtcat cgggcgcaag
      961 ctgatggccc gcctgggcac gcgcgtgggg cgcacggttg ggttcgtccc gtggacgggc
     1021 cggccggtca ccatccctcg gttggatcct ctgagcgagt tcgtggcaac ggccgaatgg
     1081 gatgctgcga tcgcccgccc gcctttgatc ttgccggccg gaccggcgcc gcagaccagc
     1141 gatgctccac ctcgcctact ggtcaaggtc gccaccacgg accgaggctg gcacgagccc
     1201 gcccagcccg accggccgtt gcggagttgg cttggctggc caacgcgttg gcaccgagaa
     1261 ggtcacgcgg cttcgcctga tatcggttgg gctgtcggca ccaagggcat tcagtagcgc
     1321 acattcactg accggccatc cgccgcgtgc caagggaaag cgcggttttg gccaatccgt
     1381 atcctatggc ccctatgaac tcgccgagcg tcgcagccag tgcgcgcgcg actggactac
     1441 cggcgcctcg gcgacggtgg ctgcgcacag tccgaatcgg tctcgccgcc gttgtggtcg
     1501 cgttcctcct ggcctacgcg gtcggccaga gggggcatac gacctggacc aggggcacga
     1561 ttctcgcggt cgaacaggac ctggtcggag ccatctggcc gttggcccag agcttcgact
     1621 cgccccgagg gtacggatac acgggcgata acccctaccc gcacctgatc cagggagact
     1681 tcggcgggac cgcgttctat ccgcccgggt acccattcct gctgtggatc accgcccgcg
     1741 cgctcgggga tcccatgcgc gcgacgcagc tcatcaacgc ggtggctggt gtgctcttca
     1801 ttctggcgag ctattggctt gccgtgggct ggacgggcaa gcgcggcctc gcattgctgt
     1861 ccgcggcctt cgtggctggc ggagcggtct ttctgactct cgcctatgcg gccagcaccg
     1921 atctcctggc gggtgcggcc tccacggctg ccctggcctg caccgcctgg gcgctgcgaa
     1981 gcccgactcg tcgccgctgg gtgctgctgg ccggcatcac cctcggtctc gcctttctgc
     2041 tccgccggca gacggcggtg ctgctcgcgc cgcttatcat cctcgtcgtc atgtctgccc
     2101 gcggacgacc tcgcgaggcc gtcatccgcg cgggcatgct cgtgggcgtc tttgggctgc
     2161 tggccttgcc cgacctgctg atccagaaca gcctccacgg cagccccctg tatggcgcgc
     2221 acggcaagaa catttggaac gcggtcgtca accgctacga ctggccggcc tggcactccg
     2281 tcccgaacac gatctcggcg ctgagcgtca tagtttccgc accggcggcc ttcgtcggca
     2341 actggttcgg gaatctcctg tactacgcgg agcggctgtg gctcgggccg gcgacgctcc
     2401 tcgccctgcc cggggcatac ctgctctggc gccgtcggtg gctgggcaag atgacggccg
     2461 caatcctcgg cagcgctgcc ttcgtggtca tcgtggcgtc cgtctctgag cgcggcgatc
     2521 gccatgccct gatcctggtt ccgaccctgg cggtgccggc ggccgctgcc ctctggtacg
     2581 ccgtgtcgcg cagatggcgc cttcccgtgg ccgccgccat cgtgatcctc gccgcatcgg
     2641 tgggaatcgc ccacaccgcg gtgaacatca ccgtggacct gcgagtgccg cacctggatg
     2701 aggtcgtggc ggccctccat gaggacggtg cccagccgga cgacgtgctc acctcgttca
     2761 attcggcgca ctacgcgttc tatgcgggca tcggcttcgt gcccctgtcg ggcgaggtct
     2821 acacctggga cgacctccgc cggttcatgg atgagcacgg cgtccggtac tacgtggcac
     2881 gcgggatcgg attccacgtc cggcgactga cggagtccct cgagcccacg gcagccccgg
     2941 attggatcga tccgatctgg gtctcgcagc ccgatcgcga caacgggcga atcgtgctct
     3001 atcggtacga cggcggcccc tgagcgggtc gtgcgctatt cgccgatgtc caccaccagg
     3061 agcgcgttct gcccttcgaa cctgaggacc gacatcaggt agcgcccgtc gccgacggcc
     3121 tcgataccgg tgacgaaccc gttgaggccc tggagcatgg tcaccgccga ccgctcgtcc
     3181 gcgacgtatg gcccgtcgcc gtcgaaatcc agccggacga gatcgagccg actcgcgctg
     3241 ccgaccagca cgccggtcgc cccgtcgacc gcggaccagt cggtgccggt gctcccgatg
     3301 gtgtccagaa tccagagcgg ttcctgcgtg gaggtgccgt cgaacgtgcc gtgtgcggga
     3361 tagccgtaat cggcaccgtc gacgacctcg atcagctgct cgtgccgcca gccgcgaccc
     3421 gtctgaccat cattgtccac gccatacagg tgaccatcgg gatcgaacgt caggctgtag
     3481 atgttcctga tccccgacgc gatgacctcg acatctccac cagccgggag aaaccgcacg
     3541 atcgtgccca acaggtcggc attcggatgg gtgatgtcct ccgccagggc aaggtcgaga
     3601 gggagcctgt ctgggccggc gatcggcatg tacaggtagc cgtcgggacc gagcgtgatc
     3661 gagttgggcg cgtgctcggt gttcacgacc ggcaggtcgg tcagcacctc tctgggctca
     3721 ccgagcgagc catcggtttg gatctcaaat gccagtacgc tcgccgagct ggcgttgatc
     3781 cggtcgagct cctcggccgg ttcaggcgtc cagcattgcg ggaacggatc cgggcaggcc
     3841 agggtaccga ggtcagccac gaacagctcg ccatccacga tcgccgggcc gcgcgggaag
     3901 ctcagacctt cggccacgac ctcgacattc gtccccggca ggccgtcctg gtcgagctcc
     3961 atgcggacga tgcgcccgtc gccgagggtc acgtatgccg acgcatggtc ctcggtgatc
     4021 acgaggccgg tcggcccccc gtcgagcgga aactcctcga ccaccgcagc accctgtccc
     4081 tggcgtgtgg tcgcggccgc cgtctggccg aagacaaatg atgttccgac gatggccact
     4141 ccggccccgc ccaggaggac ggcgcggagg acgtcggcaa ccgtagcccc gccatctgaa
     4201 tcctccgccg tcgcggcgct ctggcgctga acccgccgga cggcccaacc cgggatctcg
     4261 ctcgctccga agtggacgac cgcgaccgcg atcaggaagg cggctccgat ggcgatgtac
     4321 gttgccgagg gaggcaggcg aaattcgccg gatgcgaggc ccccacccgc gaagcgggag
     4381 aggagccggc cgctttcgcc cgcgaacagg accaccagaa cgacggtcaa cccgatcgcc
     4441 acgaccagcg gcacgacgag gaacgggcca acccgtcgac ccgggaggtg ggaggccagg
     4501 gcatgcacga ccatgagcag ggccgacaga tagccgaaga ggaggagaac ggggaaggcg
     4561 atgggcgcaa tcgagatcac ggtcgcggca tggtagcgcc tcgccgtcgc accgaccgtt
     4621 gcccgtccgg tttggcgccg cgggatttgc cgcgtcatgc cgcggtggat atgctccacg
     4681 ggcgctcagg ctgcctccat cggacatccg ggcgcggcga gcacggcgga gtagctcagt
     4741 ggtagagcag gggactcata agcccttggt cggtggttcg aatccgccct ccgctaccag
     4801 tctcctccgg accgacgacg tgcccagccg tgccgtcgcc gaggcggcga cgagactgcc
     4861 gattcccgac ggcgcgacca tggtcttggc cgtttcgggc gggcccgatt cgatggccct
     4921 cctccacggc gcccgggcgg ttgcgccttc gcacagctgg cagctggtcg tcgcccatct
     4981 ggaccacggc ctgcgacctg actcggccga tgacgcgacc ttcgtgaccg atgccgcgga
     5041 tgagctcggc ctgccgtggc ggctgcgtcg gaccgatgtc gccgcggcgg cagctgcgga
     5101 ccgactcggg atcgagggcg ccggccgtgt cgcccgctac gcgttcctgg acgaggtcgc
     5161 cgacgggctc ggctccgcga ccctggtggt taccgcccat tccgctgacg accaggccga
     5221 gactgtgctc atgaacctcg cccgtggcag cggcctgcac gggctgcggg gcatcccagc
     5281 gcgtcgcggg cgtgtgatcc gtccactcct ggccacgcgt cgcagggcgc tccggacggc
     5341 gctcgacgag gccgggatcg cctaccggct ggacccgacc aacgccgatc cggcgcatgc
     5401 ccgcgcgcgg gttcgcgccg acctggtgcc cgcgatggag tcactcaatc cggctgccgt
     5461 cgacgccgtg gtgcggttcg ctgccctggc ggctgacgat gatgcgctgc tggacgcgct
     5521 cgcggccgac gagctccggc ggcgggggac cgacggtgcg ctcgactggc acgaccctcc
     5581 gcggccggcc ctcggccgga gggtgctgcg cctcgcaatt ggcgatccgg cgccatccgc
     5641 ggaccgcatt gaggcggtgc tcgaggccgc agcgggtcct cggggcggga tcaccatcga
     5701 gcttggccgt ggccggacgg cggaaatccg cggacgacgg atcgtcatcg gtctgtaagc
     5761 cgatcggcct agcccattcg agcccatttg tcgcctatag agggcatccc tatactctgg
     5821 gcccggtcgc tgaccgttcc ccctccgtgc actggagcat tgccgccgtc ccgtgaatag
     5881 ccgtctcgtc cgcagcagca tcctcatcgt tggcgccctg ttcgccatgg ccctgctgtg
     5941 gacgtacctg atggatacgc aggagggtcc ggcctacacc tacagcgacc tcctgaccga
     6001 tgcggcggcg ggcaacgtca ccggtgtcac tcaggacggg acgacgctca ccgtgtcgtt
     6061 cgagggctcg gacacgacca agaccgcgat cgttccgtcg gaccaggtga acgtctttgc
     6121 cgaggtctgc gccgcctccg gcgcgaccgg gctcgccgac tgtgacatca cctacgaggc
     6181 ggtggccgag agcgcgaccg gccaatg
//
LOCUS       DASYGU010000055         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_882595, whole genome shotgun sequence.
ACCESSION   DASYGU010000055 DASYGU010000000
VERSION     DASYGU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882595"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VGO32_01490"
     CDS             complement(<1..1052)
                     /locus_tag="VGO32_01490"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV7199457.1"
                     /translation="MTNQPTVAEAPNIWAVAQQQFDQAADKLDLDSGLRNVLRVPKRE
                     LTVHFPVTMDDGHVEVFTGYRVQHNLSRGPAKGGIRYHQDVTLDEVRALAMWMTWKCS
                     VVNIPYGGGKGGVIVDPKQLSLRELEGLTRRFATEISPLIGPDRDIPAPDVNTNAQTM
                     AWIMDTYSMHNGYTISGVVTGKPIAVGGSLGRNEATARGAVFCLQQASKVLDLPLAGA
                     TVAIEGYGNAGSIAAQLLAAEGSTIVAVSDSTGGIHNPAGLDPAKVGAWKQEHGTVVG
                     FPGSDEVTNEELLTLACDILIPAALENQISGPIAERIQAKIVAEAANGPTMPEADDIL
                     YERGVFLIPDILCNAGG"
     gene            1259..2209
                     /locus_tag="VGO32_01495"
     CDS             1259..2209
                     /locus_tag="VGO32_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199458.1"
                     /translation="MVRSPNTRGLRIAAPAATLFILLAAVAGPVAAHHPGGSWGTAVS
                     AESPPGTSSELNTSFNDGCPIEGPDGRTLYTATTRPGAVGGGGIDIWVATRPNTSAPF
                     GAPVPLPAPINSPADDFCPTPIIGGGLFFVSSRAVPGACGLPPTGDIYFAWNRPGAGW
                     TEPRNLGCQADGGPNSALGEAGPSYFFARGHGMLYFSSGPDIYQSVQRWNGSFGPATP
                     VAELNDPVAGDFRPNVRLDGLEVVFDSNRLGSFGFQDIYVATRARTSDPWSTPANLGL
                     NINTAAGSETRASFSLFANRLYVGRTPGPEGGTDIFVSTR"
     gene            complement(2282..>2661)
                     /locus_tag="VGO32_01500"
     CDS             complement(2282..>2661)
                     /locus_tag="VGO32_01500"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7199459.1"
                     /translation="GLTPIARITGYAQADVAPEWLFEAPIEGVQRLVDKVGIPLADFD
                     LIEINEAFAAQVLADGNALGFDWDKVNVNGGAVALGHPIGASGARVLTTLLYALRHRG
                     GTRGLATLCLGGGGAVALAVELV"
BASE COUNT          404 a          911 c          917 g          429 t
ORIGIN      
        1 ccgcccgcgt tgcacaggat gtccgggatc aggaacacgc cacgctcgta caggatgtcg
       61 tcggcctcgg gcatggtcgg cccgttggcg gcctcggcca cgatcttggc ctggatccgc
      121 tcggcgatcg gcccgctgat ctggttctcg agcgcggccg ggatgaggat gtcgcaggcc
      181 agggtcagga gctcctcgtt ggtgacctcg tcggaacccg ggaaaccgac gaccgtgccg
      241 tgctcctgct tccatgcacc gaccttggcc gggtcgaggc cggccggatt gtggatgccg
      301 ccggtcgagt cgctgaccgc gacgatggtc gagccctcgg cggcgagcag ctgggcggcg
      361 atcgagccgg cgttgccgta gccctcgatg gcgaccgtgg ccccggccag cggcaggtcg
      421 aggaccttcg aggcctgctg gaggcagaac acggcgccgc gggcagtggc ctcgttccgg
      481 cccagcgacc cgccgactgc gatcggcttg ccggtcacca cgcccgagat cgtgtagccg
      541 ttgtgcatcg agtaggtgtc catgatccag gccatggtct gagcgttggt gttcacatcg
      601 ggcgccggga tgtcccggtc gggaccgatg agcgggctga tctcggtcgc gaaccgccgg
      661 gtcaggccct cgagctcacg gagcgagagc tgcttggggt cgacgatgac gccgcccttg
      721 ccaccgccgt acgggatgtt cacgacggag cacttccagg tcatccacat ggccagcgcc
      781 cggacctcgt ccagggtgac gtcctggtgg tagcggatgc cgcccttggc cggacctcgc
      841 gacaggttgt gctggacgcg gtagccggtg aacacctcga catgcccatc gtccatcgtc
      901 accgggaagt ggaccgtcag ctcccgcttc ggaacgcgga ggacgttgcg aaggccggag
      961 tcgaggtcga gcttgtcagc ggcctggtcg aactgctgct gggcgaccgc ccagatgttc
     1021 ggggcctcgg cgacggtcgg ctggttggtc atggggggcg gatgctctcc ttggcagagg
     1081 gtttgtcacg gaacggtacg ggtggggcgt acccggcgaa aaggcgcgcc gattatacgg
     1141 gccgccaggc ggcatcggtc tagaccgcct ccggattatc gaaccatctt cctcacgccc
     1201 ggcatctacc ccctgagtgg acgcgttcca aggcgtccac ctgcggaggt gcacggacat
     1261 ggtcagatca cccaacactc ggggacttcg gatcgcggcc ccagcggcca cgctcttcat
     1321 cctgctggcg gccgtcgccg gaccggtggc ggcccatcac ccgggcgggt cgtgggggac
     1381 ggcggtcagc gccgagagcc cgccgggcac gagtagcgag ttgaacacgt ccttcaacga
     1441 cggctgcccc atcgaagggc ctgatggccg aaccctgtac accgcgacga cccgtccggg
     1501 cgccgtgggc ggtggcggca tcgacatctg ggtcgccacc cgtccgaaca cgtcggctcc
     1561 gtttggggct cccgtgccgc tgccggcccc gatcaacagc ccggccgacg acttctgccc
     1621 gaccccgatc attggcggcg gtctgttctt cgtcagctcg cgcgcggtcc ccggggcatg
     1681 cggccttccg cccactggcg acatctactt cgcgtggaac cggcctggag cgggctggac
     1741 cgagcccagg aacctcggtt gccaggctga tggtgggcca aacagcgcgc tcggcgaggc
     1801 cgggccgtcg tacttcttcg cccgaggcca cgggatgctg tacttctcga gcggaccgga
     1861 catctaccag tccgtccagc ggtggaacgg cagcttcgga ccggcgacgc cggtggcgga
     1921 gctcaatgac ccagtcgccg gggacttccg gccgaacgtc cgcctggacg gtttggaggt
     1981 cgtgttcgac tcgaaccgcc tcggctcgtt cggcttccag gacatctacg tcgcgacgag
     2041 ggcccgtacc agcgacccat ggtcaacccc agcgaacctg ggcctcaaca tcaatacggc
     2101 cgcgggctca gagacccgtg cttcgttcag cctattcgcg aaccggctct acgtcgggcg
     2161 aacgcccggg ccggaaggcg gtacggacat cttcgtcagc acccgctgac ctaacccaag
     2221 agcggtcctg gagaggtggc gttcgcgcca cctctcctca tttcacaggg aggaccgacc
     2281 gtcagacgag ctctaccgcc agggccaccg ctcccccgcc gccgaggcac agggtcgcca
     2341 gaccccgggt gccgccccga tgacggagcg cgtacaggag ggtggtcaga accctggccc
     2401 ccgacgcccc gatcggatgg ccgagggcaa ccgcgccacc gttgacgttg accttgtccc
     2461 agtcgaaccc cagggcattg ccatcggcca ggacctgggc cgcgaacgct tcgttgatct
     2521 cgatcaggtc gaagtcggcc agcgggatgc cgaccttgtc caccagccgc tggacgccct
     2581 cgatcggggc ctcgaacagc cattccgggg cgacgtcggc ctgggcgtag ccggtgatcc
     2641 gggcgatggg cgtgaggcca t
//
LOCUS       DASYGU010000056         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1499660, whole genome shotgun sequence.
ACCESSION   DASYGU010000056 DASYGU010000000
VERSION     DASYGU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499660"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGO32_01505"
     CDS             <1..455
                     /locus_tag="VGO32_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEV7199460.1"
                     /translation="PRVGLWGRSRTLGRGGRIAAISGRTGRITLEADGARLVVPDADV
                     EIIDEPGSTPPARDLEADELRRKAAADLSPALDLRGERVEAALERLAAYLDQALLAGA
                     DEAVIIHGAGTGALRRAIRDTLAEHPRVRAQRSGRREEGGDGVTVAEL"
     gene            complement(452..3247)
                     /locus_tag="VGO32_01510"
     CDS             complement(452..3247)
                     /locus_tag="VGO32_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV7199461.1"
                     /translation="MTSSPPPSEGYPLPPRTGAAPRMRVQPLPALIFATFGVLAGGIV
                     VLAVMLYASYASSLPDVTELEDYAPDEGSVVMSADEQELATFAETNRRVVDYDDIPTV
                     IVDATVAAEDHTYWENPCVDPRAIVRAMLQNFSAGEVVSGASTICQQLVRSVLLPPDL
                     MADPDRQLERKIKEAMLALELDSAYPGQEGKEQIMEFFLNEMYYGNNGYGIWAAVDRY
                     YGKDFLQIQGTPTEPGASPAPSVAPTPAPSASGSPEPAPPDPNEVTAGEAAMLAGLLR
                     APSELDPTQYGVPAVDEADQPIPGVLEVPSDAQPVIIRDDVLSDMVLLDYLTDAERNE
                     TIEAPTYVLVPETPAYLAPHFVYATRREVADLLGSEDIIDRGGLTIETTLEYEGYQVA
                     AEKWAQVVYDMDRLTDAELTARYGAAAMGWIDQLQGRNIGNDAIVSLNYRTGAVVAYV
                     GSANFNGEATPQHQPQYDVVGQAFRQSGSAFKPITYATGFETGTITPATMFMDVETEI
                     VPGYEVHDADNNERGPVRVRDALKYSLNIPVTKAQQMIGTERVVEQAERLGLEWDPNQ
                     DPNVASLTLGTIGVRMIDLAAAYGALANGGIYREPYLVQRIVDRDGVVIYDRETDGPD
                     QVQAISPEAAYLTTDILADNTDPAENPLWGPRFQLLTDAGRRPATLKTGTTTDFKDLQ
                     AFGFLAADADPTIDEGAIMTGVWVGNSDFSAIDSVFAADGPTFIWHDYMAEVTALNAL
                     PIRDFVRPEAIVDREVDAMSGMAPGEFTTTTLTELFMASGPGLTTDDTHVELIIEAAT
                     GKIWQEGCGDFVPATPGPTSSPPPSGEPVPDPGPIEPRLRIFLDLSGWDDQNPAWEQS
                     NVAWIGTWRGRESSVPRFPIVRLDRTLAPTETCTPGAVPTSTPTPEATPTPEATPTPA
                     PTDTPAPTPTPVESPAP"
     gene            3315..>3824
                     /gene="tyrS"
                     /locus_tag="VGO32_01515"
     CDS             3315..>3824
                     /gene="tyrS"
                     /locus_tag="VGO32_01515"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV7199462.1"
                     /translation="MDRDVAIEAVLSRRVAQVVEADALRGMLRGDRPLRVKLGIDPTS
                     ADLHIGHAVVLERLRAFQELGHIAVLVVGDTTAQIGDPSEKNVTRPMLTREEVHANAE
                     TYLAQFHLIVDPARTEVRWQSEWFDEFRLREVFALMSRFTLARMIERDTFAKRLAEGA
                     PVSMHETMYP"
BASE COUNT          543 a         1243 c         1419 g          619 t
ORIGIN      
        1 agccgcgagt cgggctatgg ggtcggagcc ggaccctggg ccggggtggg cgcatcgcgg
       61 cgatcagcgg ccgaacgggg cggatcacgc tcgaggccga cggcgcccgg ctggtcgtgc
      121 ccgacgcgga cgtggagatc atcgacgagc ccggatccac gccgccggca cgtgatctcg
      181 aggcggacga gctgcgccgc aaggcggcgg ccgacctgag cccggcactc gatctccgcg
      241 gcgaacgggt cgaggccgcg ctcgagcggc tggccgccta cctggaccag gcgttgctcg
      301 ccggcgcgga cgaggcggtg atcatccacg gggccgggac gggcgccctg cgacgggcaa
      361 tacgggacac gctggcggaa catccgcgcg tgcgtgctca gcggagcggt cggcgcgagg
      421 aaggcgggga cggtgtgacg gtcgccgagc tctagggagc tggactctcc acgggcgttg
      481 gggtgggagc gggtgtatcg gtcggggccg gcgtcggcgt ggcctcgggt gtcggggtcg
      541 cctccggggt cggcgtggac gtcggcaccg caccaggcgt gcatgtctcg gtgggggcga
      601 gggttcgatc gaggcggaca atcgggaagc gcggcacgct cgactctcgt ccccgccagg
      661 tgccgatcca ggccacgttc gactgctccc aggccgggtt ctggtcgtcc cagccggaca
      721 gatcgaggaa tatccgcagc cggggctcga tgggcccggg atcgggaacc ggctctccac
      781 tcggcggcgg actggaggtc gggcccgggg tggccggcac gaagtcaccg cagccctcct
      841 gccagatctt cccggtagcc gcctcgatga tcagctccac gtgcgtgtca tcggtggtca
      901 ggcctgggcc gctggccatg aacagctcgg tcagggtcgt ggtcgtgaac tcgccgggtg
      961 ccatgccgct catcgcatcg acctcgcggt ccacgatcgc ctcgggccga acgaagtccc
     1021 ggatcggcag cgcgttgagg gccgtgacct ccgccatgta gtcgtgccag atgaaggtcg
     1081 gcccgtccgc ggcgaagacc gagtcgatcg ccgagaagtc gctattgccg acccacaccc
     1141 cggtcatgat cgcgccttcg tcgatcgtgg gatccgcgtc ggcggccagg aaaccgaatg
     1201 cctggagatc cttgaagtcg gtcgtggtgc cggtcttgag ggtcgcgggc cgccggccgg
     1261 catcggtcag cagctgaaag cgaggacccc agagcgggtt ctcggccgga tcggtgttgt
     1321 cggccaggat gtcggtggtc aggtaggccg cctcggggga gatggcctgg acctggtcgg
     1381 ggccgtccgt ctcgcggtcg tagatgacga ccccgtcccg gtcgacgatg cgctgaacga
     1441 ggtatggctc gcgatagatg ccgccgttgg ccagcgcgcc gtaggcagcg gccaggtcga
     1501 tcatccggac cccgatcgtg cccagggtca gggacgcgac gttcgggtcc tggttgggat
     1561 cccattccag tcccagccgc tcggcctgct cgaccacgcg ctcggtgccg atcatctgct
     1621 gcgccttggt caccgggatg ttcagcgaat acttcagcgc gtcgcgtacg cggaccgggc
     1681 cgcgctcgtt gttgtccgcg tcatggacct cgtagccggg cacgatctcg gtctcgacgt
     1741 ccatgaacat ggtggccggc gtgatcgtgc ccgtctcgaa gccggtcgcg taggtgatcg
     1801 gcttgaaggc cgagccggac tggcggaatg cctggcccac gacgtcgtac tgcggctgat
     1861 gttggggcgt ggcctcgccg ttgaagttcg cgctgccgac ataggcgacg accgccccgg
     1921 tccggtagtt cagcgagacg atggcgtcgt tgccgatgtt ccggccctgg agctggtcga
     1981 tccagcccat ggcggccgcg ccgtaccgcg cggtcagctc cgcatcggtc aggcggtcca
     2041 tgtcgtatac gacctgcgcc cacttctcgg cggcgacctg gtagccctcg tactcgaggg
     2101 tggtctcgat ggtcaggccg ccgcggtcaa tgatgtcctc cgagccgagc aggtcggcca
     2161 cctcgcgccg ggtcgcgtac acgaagtgcg gggccaggta tgccggggtc tcgggcacca
     2221 gcacataggt gggcgcctcg atggtctcgt tccgctccgc atcggtcagg tagtcgagca
     2281 ggaccatgtc gctcaagaca tcgtcacgga tgatcaccgg ctgcgcgtcg ctcgggacct
     2341 cgagcacgcc cgggatcggc tggtcggcct cgtccacggc cgggacgccg tactgggtgg
     2401 ggtccagctc ggacggggcg cggagcaggc cggccagcat ggcggcctca cccgcggtca
     2461 cctcgttcgg gtcgggcggt gcgggctcgg ggctgcccga ggcgctcggc gccggcgtgg
     2521 gggcgacgct cggcgcgggg ctggcgcccg gctcagtcgg tgttccctgg atctggagga
     2581 agtctttccc gtagtagcgg tccacggcgg cccagatccc gtatccgtta ttgccgtagt
     2641 acatctcgtt caggaagaac tccatgatct gctccttgcc ctcctggccc gggtaggccg
     2701 agtcgagctc aagcgcgagc atggcttcct tgatcttccg ttccagctgg cggtcggggt
     2761 cggccatcag gtccggtggc aggaggacgc tgcggacgag ctgctggcag atggtcgatg
     2821 caccggacac gacctcgccg gcggagaagt tctggagcat ggcccgcacg atggctcgcg
     2881 ggtcgacgca cgggttctcc cagtaggtgt ggtcctcggc ggcgaccgtc gcgtccacga
     2941 tcaccgtcgg gatgtcgtcg tagtcgacga cccgtcggtt cgtctcggcg aaggtggcca
     3001 gctcctgctc gtcggccgac atgacgaccg agccttcgtc gggggcgtag tcctcgagct
     3061 ccgtcacgtc gggcagcgag ctggcgtagg aggcgtagag catcacggcg agcaccacga
     3121 tcccgcccgc gagcacgcca aacgtggcga agatcaatgc ggggagtggc tggacgcgca
     3181 tccgcggcgc ggcgccggtc cgagggggca atgggtagcc ctccgacggc ggaggtgacg
     3241 aggtcacggc ggcgaactct agcagagcgg ccccgatggc gcccgatggc gaacgggccg
     3301 ggtaccatcg gcgcgtggat cgtgacgtgg cgatcgaagc cgttctcagc cgccgggttg
     3361 cacaggtggt ggaggccgat gccctccggg gcatgctccg cggagaccgc ccgctgcggg
     3421 tcaagctcgg gatcgatccc accagtgccg accttcacat cggtcacgcc gtcgtcctcg
     3481 agcgccttcg ggcgttccag gaactcggcc acatcgcggt cctggtggtg ggggacacga
     3541 cggcccagat cggggatcca tcggagaaga acgtcacccg gccgatgctg acccgcgagg
     3601 aggtgcacgc caatgcggag acctacctcg cccagttcca cctgatcgtg gatcccgccc
     3661 ggaccgaggt gcgctggcag tcggagtggt tcgacgagtt ccggctgcgc gaggtcttcg
     3721 cgctcatgag ccggttcacg ctggcgcgca tgatcgagcg cgacacgttc gccaagcgcc
     3781 tggccgaggg cgccccggtc tcgatgcacg agacgatgta cccg
//
LOCUS       DASYGU010000057         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_677242, whole genome shotgun sequence.
ACCESSION   DASYGU010000057 DASYGU010000000
VERSION     DASYGU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677242"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VGO32_01520"
     CDS             complement(<1..766)
                     /locus_tag="VGO32_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV7199463.1"
                     /translation="MTSEPNGRMIAGRYRLISPEGEGGMATVWRAMDEQLEREVAVKI
                     LRPQFGADPGFAARFKNEARAAGSLSHPNIVQVYDFGTDTESGDQYIVMQLVEGQDLA
                     TVLKERGPLEVDEAVAIGASVADALDAAHRQGLIHRDIKPGNILITPGGRTLVTDFGI
                     ARAVAEASMTVTGTTIGSVHYFSPEQAAGEEVGPTSDIYALGIVIYEMLSGRRPFEGE
                     TAAGVALKRLNEPPPPLSTGVRPIPAPLEAVVMQSLA"
     gene            complement(773..1678)
                     /gene="galU"
                     /locus_tag="VGO32_01525"
     CDS             complement(773..1678)
                     /gene="galU"
                     /locus_tag="VGO32_01525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV7199464.1"
                     /translation="MTAISEEVRITPRVRKAVIPAAGYGTRFLPATKAIPKEILPLVD
                     RPVILYAVEEAVASGIEQIIIVVGSGTSVIEEHFDSHPALERWLEDHGDIERLRAVRR
                     QSEIAAIAYVRQKEPLGLGHAVLMAKELVGDEPFAVLLPDDVMVNPGGAPVMAQLIEA
                     HTAHRGSVIAITKVPPTDTSRYGIVRTTRGEGRLWELADLVEKPPADEAPSDLGVLGR
                     YVLSPRVFDKLEHTQTGAGGEIQLTDAIRALIADQPVYGYEFTGYRHDAGTRLGWLEA
                     NVALALESDLADDFRAYLRGLDLRD"
     gene            1789..>1994
                     /locus_tag="VGO32_01530"
     CDS             1789..>1994
                     /locus_tag="VGO32_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199465.1"
                     /translation="MVRSLWRTLMGPSRDARMRYISRPLPRDLPDAQVVEALDRALAV
                     NDDPYYLVETLPAALREVTGRDFEV"
BASE COUNT          277 a          676 c          720 g          321 t
ORIGIN      
        1 gggccagcga ctgcatgacc accgcctcga gcggcgcggg gatgggccgg acgccggtgg
       61 acagcggcgg gggcggctcg ttcagccgct tgagcgccac accggccgcc gtttcccctt
      121 cgaacggacg gcggccgctc agcatctcgt agatgacgat gccaagggcg tagatgtcgg
      181 acgtcggccc gacctcttcg ccggcggcct gctcggggct gaagtagtgg accgagccga
      241 tcgtcgtgcc ggtcacggtc atcgacgcct cggccaccgc ccgcgcgatg ccgaagtcgg
      301 tgaccagggt cctaccgccg ggcgtgatca ggatgttgcc gggcttgatg tcccgatgga
      361 tcaggccctg gcggtgtgcg gcgtcgagcg catcggccac cgaggcaccg atggccaccg
      421 cctcgtccac ctcgagcggt ccgcgctctt tgaggacggt ggccaggtcc tggccttcga
      481 ccagctgcat gacgatgtac tggtcgccgc tctcggtatc ggtcccgaag tcatagacct
      541 ggacgatgtt cgggtgggag agcgatccgg ccgcccgggc ctcgttcttg aaacgggccg
      601 caaagcccgg gtcggcgccg aactgggggc ggaggatctt caccgccacc tcgcgctcga
      661 gctgctcgtc catcgcgcgc cagaccgtgg ccattccgcc ctcgccctcg gggctgatga
      721 gccgatagcg tcccgcgatc atgcggccgt tgggttcgct ggtcatggcc ctctagtctc
      781 gcaggtcgag gccgcgaagg taggcacgga agtcgtccgc caggtccgac tcgagcgcca
      841 gcgcgacgtt ggcctccagc cagccgagcc gggtcccggc gtcgtgccgg tagccggtga
      901 actcgtagcc gtagaccggc tggtcggcga tgagcgcccg gatcgcgtcg gtgagctgga
      961 tctcgccgcc cgccccggtc tgggtgtgtt ccagcttgtc gaacacccgc gggctcagga
     1021 cgtaacggcc caacacgccc aggtccgacg gcgcctcgtc ggccgggggc ttctcgacga
     1081 ggtcggccag ctcccacagc cggccctcgc cacgggtggt cctgacgatc ccgtagcggc
     1141 tggtgtcggt gggcgggacc ttggtgatcg cgatcaccga cccccggtgc gcggtgtggg
     1201 cctcgatcag ctgggccatg accggggcac cgccggggtt gaccatcacg tcgtcgggca
     1261 ggagcacggc gaatggctcg tcgccgacca gctccttggc catcagcacc gcgtggccca
     1321 ggccgagcgg ctccttctgg cggacgtagg cgatggcggc gatctccgac tggcgtcgga
     1381 cggcccggag gcgttcgatg tccccgtggt cctcgagcca gcgctcgagg gcggggtgcg
     1441 agtcgaaatg ctcctcgatg acgctcgtgc cggagccgac cacgatgatg atctgctcga
     1501 tccccgaggc cacggcctcc tcgaccgcgt acaggatcac cggccgatca acgaggggca
     1561 ggatctcctt ggggatggcc ttggtcgccg gcaggaaccg ggttccatag ccggcggcgg
     1621 gaatgaccgc ctttcggaca cgcggagtga tgcgaacttc ctccgagatg gctgtcagtc
     1681 gtgcgggtcg gtcggaccgg gcgggtggac ggacgcgctc acgcggcggc cagtataccg
     1741 atgggtgggc gacgggctga cggcgcgcat cggtacaatc ccggtccgat ggttcggagt
     1801 ctgtggcgca cgctcatggg tcccagccgg gatgcccgga tgcggtacat cagccgcccg
     1861 ctgccccgcg acctgcccga cgcccaggtg gtcgaggccc tcgatcgggc actggccgtg
     1921 aacgatgatc cgtactacct ggtcgagacg ctgccggccg ccctgcgcga ggtcaccggt
     1981 cgggacttcg aggt
//
LOCUS       DASYGU010000058         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1500094, whole genome shotgun sequence.
ACCESSION   DASYGU010000058 DASYGU010000000
VERSION     DASYGU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500094"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGO32_01535"
     CDS             <1..328
                     /locus_tag="VGO32_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199466.1"
                     /translation="LQEQQAALLREFVGNAEDALRLTGDLVLLDESDDQLVMRGDGWF
                     ETTLNLADMAPRSAAEPMEAHRDGLTVTDAKTVAEFYDPADVLQWVTEALNERYPAVD
                     FSAVYD"
     gene            354..983
                     /gene="pyrE"
                     /locus_tag="VGO32_01540"
     CDS             354..983
                     /gene="pyrE"
                     /locus_tag="VGO32_01540"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV7199467.1"
                     /translation="MSRGVPLPANLPAASMEDLFRDAGALREGHFRLKSGLHSPRYLE
                     KFLVLQYPTLASELCRRMAHEVASLAPNVVVGPTTGGVLLAFETARHLSAILGWEVRG
                     LFAEPSTLAGARELRRGFEVSRGDTIVLVDDILTTGASLRETGEAVERAGGQATAAVV
                     MVDRSAEPVDIGIPIRSVGRIEIPVWEPADCPLCDAGDWLETPGSAGTT"
     gene            complement(980..1861)
                     /locus_tag="VGO32_01545"
     CDS             complement(980..1861)
                     /locus_tag="VGO32_01545"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEV7199468.1"
                     /translation="MTDPGAAAPAPATADEPRTHPVLIGGAEMAEIDEAAQKLGLTQD
                     ALMESAGAAVAEVALTELARLAEPVLGPGGPLAERLLTVVLCGTGNNGGDGLVAARRL
                     AAGGNRVIAVLVGEAGRIAGAAAAHNWAVLQAMAATGSMQLFVAPSADLLASLRARLS
                     PASLLVDALLGSGASGPLREPVASAVQLVNAIRTHAAAAGRPCRVLAVDTPTLIDLTG
                     GERSDPVIAADLTVTFHRAKAGFALDPEARRLAGRYLVAPIGIPVEAEAGIVPADGEY
                     PPARITEVTWQEPIARA"
     gene            complement(1858..2382)
                     /locus_tag="VGO32_01550"
     CDS             complement(1858..2382)
                     /locus_tag="VGO32_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV7199469.1"
                     /translation="MSQGTPPPVETGHEPIPRWIVGIGAAAVLIGVISLSTGLEGTNP
                     DLVGPGPSEAAPPSGPAPSGEPPPSGEPPPSSPPGDAVALIEAAGCQACHGENLEGQG
                     AFPSLHGIADGPKSDNLQDLGTEFPDTWPNLWIDGTGPEVAGIDRGGMPVFGEGLLTP
                     EEITTIVDFLLTLE"
     gene            complement(2386..>2611)
                     /locus_tag="VGO32_01555"
     CDS             complement(2386..>2611)
                     /locus_tag="VGO32_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199470.1"
                     /translation="AAGLYHVDLVADGLTTAEINASLLPIHLVAWAGLGLLGLAAAGH
                     ATSAYLLAAHGRPVHTAVPGAAPAAAPAGH"
BASE COUNT          350 a          930 c          983 g          348 t
ORIGIN      
        1 gctccaggag cagcaggcgg cgctcctgcg cgagttcgtg ggcaacgcgg aggatgccct
       61 gcgcctgacc ggagacctcg tcctgctcga cgagtccgat gaccagctcg tcatgcgagg
      121 cgacggctgg ttcgagacga cgctcaacct ggcggacatg gcgccgcgat cggcggcgga
      181 gccgatggag gcgcaccggg acgggttgac cgtgaccgat gccaagacgg tggccgagtt
      241 ctacgacccg gcggacgtac tccaatgggt gactgaggcg ctgaacgagc gctacccggc
      301 ggtcgacttc tccgcggtct acgactgagc cggcagcgag gaagcctgga cccgtgtccc
      361 gcggcgtgcc actccccgcg aacctgcccg ccgcctcgat ggaggacctg ttccgcgatg
      421 ccggggcgct ccgcgagggc cacttccgcc tgaagagcgg cctgcactcc ccgcgctatc
      481 tcgagaagtt cctcgtcctc cagtacccga ccctggcgtc cgagctgtgc cggcgaatgg
      541 cacacgaggt ggccagcctg gcgccgaacg tcgtggtagg gcccaccacc ggcggggtgc
      601 tgctcgcgtt cgagaccgcg cgacacctgt cggcgatcct cggctgggag gtccgcggcc
      661 tgttcgccga gccgtccacc ctggctggcg cccgcgagct gaggcgcggg ttcgaggtca
      721 gccgcgggga cacgatcgtg ctcgtcgacg acatcctgac caccggcgca tccctgcgag
      781 agacgggtga ggcggtcgag cgggccggag gccaggccac ggccgcggtg gtgatggtcg
      841 accgctccgc cgagccggtg gacatcggca tcccgatccg gtcggtggga cggatcgaga
      901 tcccggtctg ggagccggcg gactgcccgc tgtgcgacgc gggggactgg ctggagacgc
      961 cggggagcgc cggaacgacc taggcccggg cgatcggctc ctgccaggtc acctcggtga
     1021 tccgggccgg cgggtactcc ccatcggccg ggacgattcc ggcttccgcc tcgaccggga
     1081 tcccgatcgg cgcgacgagg taccggcccg cgagccgccg ggcctcgggg tccaaggcga
     1141 acccggcctt cgctcggtgg aaggtcacgg tcaggtccgc ggcgatgacc ggatcggagc
     1201 gttcgccacc ggtcagatcg atcagagtgg gcgtgtccac ggccagcacc cggcacggac
     1261 gccccgcagc tgcggcgtgg gtgcggatcg cattcaccag ctggacggcg ctcgcgaccg
     1321 gttcgcggag cgggccgctg gcgccgctgc ccagcagggc gtcgaccagc agggacgcgg
     1381 gcgagaggcg cgcccggagg ctcgccagca ggtccgcgga cggcgccacg aacagctgca
     1441 tcgagccggt ggcggccatg gcctggagga ccgcccagtt gtgcgccgcg gccgctcccg
     1501 cgatccgccc ggcctccccg accaggaccg cgatcacccg gttgccgcca gccgccagcc
     1561 gtcgggcggc caccagcccg tcaccgccgt tgttgcccgt tccgcacagg accacggtca
     1621 gcaaccgctc ggcgagcggg ccacccggcc cgagcacggg ctctgccagc cgcgcgagct
     1681 cggtgagcgc aacttccgcg acggcggccc cggcgctctc catcagcgcg tcctgggtca
     1741 ggcccagctt ctgggcggcc tcgtcgattt cggccatctc ggcacctccg atgaggaccg
     1801 gatgcgtgcg tggctcatcg gccgtcgcgg gagcgggcgc ggcagcgccc ggatcggtca
     1861 ttcgagcgtc agcaggaagt cgacgatggt cgtgatctct tcgggcgtca gcaggccctc
     1921 gccgaacacg ggcatgccgc cgcggtcgat gccagccacc tcgggtccgg tcccgtcaat
     1981 ccagaggttg ggccacgtgt cgggaaactc ggtgcccagg tcctgcaggt tgtcgctctt
     2041 cggcccgtca gcgatgccgt ggaggctcgg gaacgcgccc tggccctcca ggttctcgcc
     2101 gtggcacgcc tggcagccgg cagcctcgat gagcgcgacc gcgtcccccg gcgggctgga
     2161 cggtggcggt tcgcccgagg gaggcggttc gcccgagggc gccggaccgc tcggcggcgc
     2221 ggcctcgctc ggtcccggac cgacgaggtc ggggttggtg ccctcgaggc ccgtgctcag
     2281 cgagatcacg ccgatgagga ccgccgccgc cccgatgccc acgatccagc gcggaatcgg
     2341 ctcgtggccg gtctcgacag gcggcggcgt gccctgcgac atcggtcagt ggccagccgg
     2401 tgcggcggcg ggcgcggccc cgggaacggc ggtgtggacc ggtcgtccgt gggctgccag
     2461 caggtaggcg gatgtcgcat gcccggcggc cgcgagcccg aggagcccga ggccggccca
     2521 cgcgacgagg tggatcggga gcagcgaggc gttgatctcg gcggtggtca ggccatcggc
     2581 caccaggtcg acgtggtaca ggccggcggc g
//
LOCUS       DASYGU010000059         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_677347, whole genome shotgun sequence.
ACCESSION   DASYGU010000059 DASYGU010000000
VERSION     DASYGU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677347"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VGO32_01560"
     CDS             complement(<1..766)
                     /locus_tag="VGO32_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV7199471.1"
                     /translation="MTGETGGRVIAGRYRLISPEGEGGMATVWRAMDEQLEREVAVKI
                     LRPQFGADPGFAARFKNEARAAGSLSHPNVVQVYDFGTDNESGDQYIVMQLVEGQDLA
                     TVLRERGPLEVDEAVAIGASVADALEAAHRQGLIHRDIKPGNILITPGGRTLVTDFGI
                     ARAVAEASMTVTGTTIGSVHYFSPEQAAGEEVGPTSDIYALGIVIYEMLSGRRPFEGE
                     TAAGVALKRLNEPPPPLSTGVRPIPAPLEAVVMQSLA"
     gene            complement(773..1651)
                     /gene="galU"
                     /locus_tag="VGO32_01565"
     CDS             complement(773..1651)
                     /gene="galU"
                     /locus_tag="VGO32_01565"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV7199472.1"
                     /translation="MTPRVRKAVIPAAGYGTRFLPATKAIPKEILPLVDRPVILYAVE
                     EAVASGIEQIIIVVGSGTSVIEEHFDSHPALERWLEDRGDIELLRAVRRQSEIAAIAY
                     VRQKEPLGLGHAVLMAKELVGDEPFAVLLPDDVMVNPGGAPVMAQLIEAHTAHRGSVI
                     AITKVPHADTSRYGIVRTTRGEGRLWELADLVEKPPVDEAPSDLGVLGRYVLSPRVFD
                     KLEHTQTGAGGEIQLTDAIRALIADQPVYGYEFTGYRHDAGTRLGWLEANVALALESD
                     LADDFRAYLRGLDLRD"
     gene            1790..2329
                     /locus_tag="VGO32_01570"
     CDS             1790..2329
                     /locus_tag="VGO32_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199473.1"
                     /translation="MVRSLWRTLMGPSRDARMAFISRPLPRDLPDAQVVEALDLAMAV
                     NPDPYYLVETLPAALREVTGRDFEVLDRSVQDVTGTHLRRAVMIRDGDVGHWPGYDAM
                     RYSLLASPEEAAAARAALSEPASRMDGWAPPGPVAEGGMDGADEPVESSAESAESAVT
                     GEAAPPADEAAPAPAEPRA"
BASE COUNT          330 a          828 c          875 g          348 t
ORIGIN      
        1 gggccagcga ctgcatgacc accgcctcga gcggcgcggg gatgggccgg acgccggtgg
       61 acagcggcgg gggcggctcg ttcagccgct tgagcgccac accggccgcc gtttcccctt
      121 cgaacggacg gcggccgctc aacatctcgt agatgacgat gcccagggcg tagatgtcag
      181 acgtgggccc gacctcctcg ccggcggcct gctcggggct gaagtagtgg accgagccga
      241 tcgtcgtgcc agtgaccgtc atggaggcct cggccaccgc ccgcgcgatg ccaaagtcgg
      301 tgacgagcgt ccgaccgccg ggcgtgatca ggatgttgcc gggcttgatg tctcgatgga
      361 tgaggccctg gcggtgggcg gcctcgagcg catccgccac cgaggctccg atcgcgaccg
      421 cctcgtccac ctcgagcggt ccgcgttcgc ggaggacggt ggccaggtcc tggccctcga
      481 ccagctgcat gacgatgtac tggtcgccgc tctcgttgtc ggtcccgaag tcgtacacct
      541 ggaccacgtt cgggtgggaa agggacccgg cggcgcgggc ctcgttcttg aatcgggcgg
      601 caaagcccgg gtcggcgccg aactgggggc ggaggatctt gaccgccacc tcgcgctcga
      661 gctgctcgtc catggcccgc cagacggtgg ccatcccgcc ctcgccctcg gggctgatca
      721 gccgatagcg tcccgcgatc acgcggccgc cggtttcgcc ggtcatggcc cgctagtctc
      781 gcaggtcgag gccacggagg taggcacgga agtcgtccgc caggtccgac tccagggcca
      841 gcgcgacgtt ggcctccagc cagccgagcc gggtcccggc gtcgtggcgg tagccggtga
      901 attcgtagcc gtagaccggc tggtcggcga tgagcgcgcg gatcgcgtcg gtgagctgga
      961 tctcgccgcc ggcaccggtc tgggtgtgct ccagcttgtc gaacacccgc gggctcagga
     1021 cgtagcgacc cagcacgccg aggtccgacg gcgcctcgtc gaccggcggc ttctcgacca
     1081 ggtccgccag ctcccacagc cgtccctcgc cccgggtggt ccggacgatt ccatagcggc
     1141 tggtgtcggc gtgcgggacc ttggtgatcg cgatcaccga cccacggtgg gcggtatgcg
     1201 cttcgatcag ctgggccatg accggggcac cgccgggatt gaccatcacg tcgtccggga
     1261 ggagcaccgc gaacggctcg tcgccgacca gctccttggc catgaggacc gcgtgtccta
     1321 ggccgagcgg ctccttctgg cggacatagg cgatggccgc gatctcggac tggcggcgga
     1381 ccgcgcggag gagctcgatg tcgccgcggt cctcgagcca gcgctccagg gcggggtgcg
     1441 agtcgaagtg ctcctcgatg acgctcgtgc cggagccgac cacgatgatg atctgctcga
     1501 tccccgacgc caccgcctcc tcgaccgcgt acaggatcac cggccgatca acgagcggca
     1561 ggatctcctt ggggatggcc ttggtcgccg gcagaaagcg ggttccatag ccggcagccg
     1621 ggatgaccgc ctttcggacg cgcggagtga tgcgaacgtt cctccgaggt gggtgtcagt
     1681 cgtgcgggtc ggtcggaccg ggcgggtgga cggacgcgct cacgcggcgg ccagtatacc
     1741 gatgggtggg cggcgagccc gcggcgcgca tcggtacaat cccggtccga tggtccgcag
     1801 tctgtggcgc acgctcatgg gtcccagccg ggatgcccgg atggcattca tcagccgccc
     1861 gctgccccgc gacctgcccg acgcgcaggt ggtcgaggcc ctcgacctgg ccatggccgt
     1921 gaaccccgat ccgtactacc tggtcgagac cctgcccgcc gccctgcgcg aggtcaccgg
     1981 gcgggacttc gaggtcctgg accggtccgt ccaggacgtg accggcacgc atctccgccg
     2041 cgcggtgatg atccgcgacg gcgacgtggg ccactggccc ggctacgacg cgatgcgcta
     2101 ctcgctgctg gccagcccgg aggaggccgc cgcggcgcgc gcggcgctgt ccgagccggc
     2161 gagccggatg gacggctggg caccaccggg cccggtggcc gaaggcggca tggacggggc
     2221 agacgagccg gtcgagtcct cggcggagtc ggcggagtcg gcggtcacgg gcgaggcagc
     2281 cccgcccgcc gacgaggctg cgccggcgcc cgcggaaccg cgggcctaga tcagccaatg
     2341 ggccggcagt cccttccgcg cgaccagtgg ctcgagatcg g
//
LOCUS       DASYGU010000060         5663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_677554, whole genome shotgun sequence.
ACCESSION   DASYGU010000060 DASYGU010000000
VERSION     DASYGU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677554"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..870)
                     /locus_tag="VGO32_01575"
     CDS             complement(115..870)
                     /locus_tag="VGO32_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7199474.1"
                     /translation="MSTSLNGRVALVTGAAHGIGAATVRLLAEHGAAVVATDVLDEEG
                     EALASELTAKGRAVTYHHLDVADESAWATVIGQTVADHGRLDVLVNNAGIGTLADVEQ
                     ETVEGWDQLTAINQRGVWLGMRAAVAEFRKVGGGSIINLSSIFGAVGGFGGSIAYHAS
                     KGAVRLMTKNAAIRFAKEGIRVNSVHPGFIDTPMVAQVKGTEIEQLIINNTPMGRMGR
                     SEEVAEVIAFLASDAASYMTGSEVYVDGGWTAQ"
     gene            1071..1472
                     /locus_tag="VGO32_01580"
     CDS             1071..1472
                     /locus_tag="VGO32_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7199475.1"
                     /translation="MDKILVAYDGTEPADRALATAIDLAKAFDATVGVVSVVPVHPGR
                     SPIDPWDDPAVHAEQLKRADIILRAAGVEATLHQQGGDPARVIEQEAEAGGYDTIVIG
                     SRGQNAVERVLQGSVSEHVATHATATVVITR"
     gene            complement(1565..1822)
                     /locus_tag="VGO32_01585"
     CDS             complement(1565..1822)
                     /locus_tag="VGO32_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199476.1"
                     /translation="MTYLQDPYRPEDEDAGESTPVGPGWTSSTFNPKVIDFGHYAGRT
                     IEEIAETDQPFLYWLARHPSGARYRTEIARVLADRRAPVEY"
     gene            complement(1869..3272)
                     /locus_tag="VGO32_01590"
     CDS             complement(1869..3272)
                     /locus_tag="VGO32_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199477.1"
                     /translation="MTHIDLTDVEPGLPAGTGSVFRNRNFVFLWSAQVLSQLASNMVL
                     AALMAVVVGATGSNTANALLILTFLVPAVVFSALAGVLVERSDARWIMILSNLGRAFG
                     CALFIALGANVFAILVLNFAISTISAFFGPAELIAITRLVERRHLMAANSIFILTVNA
                     TFGIGFGVIGPLLLTTLGPNAVYVTVSVMFALAAVAIIPLPSVKPEKTPRPFDAGKTM
                     THVLEELREGIAFVQNTPRISWSLRYLVIGASIIGVLGALGPGYATGVLNLSAEDIFF
                     IMGPAGLGAVMGILFINSFGQYIPRRLLIDVGLMLTAAMLLAIALVGPLTDILRPTLA
                     PIEEDLPDLLGPLLSVIALVIVIAFGAGIAYALIAIPAQTSLQEELPPDVRGRIFGIL
                     NTLLSVASFFPVLVAPVIADLVDIMAPGFGIPFVLLALALFMVWVGVVSWRNNAALGL
                     HQRVPPDQIPGQSPDQV"
     gene            complement(3269..3478)
                     /locus_tag="VGO32_01595"
     CDS             complement(3269..3478)
                     /locus_tag="VGO32_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEV7199478.1"
                     /translation="MAVPVELRLGDRITLRKPHPCGSHEWSVVRLGADIGLVCARCGH
                     RVLLDRLDVERRYTGHLEHGPESVS"
     gene            complement(3478..4821)
                     /locus_tag="VGO32_01600"
     CDS             complement(3478..4821)
                     /locus_tag="VGO32_01600"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV7199479.1"
                     /translation="MVGRETAEAALARIEPGSEGEATVTERDLALTRFAGSMIHQNLA
                     EHDATLRVRVRRDARTGVAVTNRLDAAGIEEVVARAAAIARHATPEADPPPFANETGS
                     DSDLGWVEATAEADPRERAEGARAVIDAGVARDLTVSGAYSIEAQRMTVANSTGLASS
                     NGVTQAKLVTVMIGPAGETGYAQAIDTDLRAIDPHAVGSEAADRTARAAEPGDLGPGE
                     YPVVLGEYAVAEVLEYLAFMAFSGLALEEGRACVELGQPAFGPNVTIWDDGLDTTGIP
                     STIDFEGVPKRRVALVTDGVARETVHDGATAYRAGVPRTGHGLPAPNTFGPLCWNLFM
                     APGDTARDRLLDGIDRGLLITRFHYVNIVHPKKGILTGMTRDGTFLIEDGAVAGPVRN
                     LRFTQSIPDAFSRISAIAAETRLVGAEYTGIASRVPAIRIDGWNFTGVTANEATV"
     gene            complement(4815..>5663)
                     /locus_tag="VGO32_01605"
     CDS             complement(4815..>5663)
                     /locus_tag="VGO32_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEV7199480.1"
                     /translation="AGFEAIREMDLGAHGQRIAEEAVALLDAPQCPSGEMDVILEASQ
                     VAIQVHESCGHPTELDRVLGMEASFAGTSFLTTDRLDRFQYGSPVVNIDADATAVGGL
                     GTFGWDDEGVRGQNVPLVSAGRFVGYLTSRETAPVIGRQSMGSSRSASWRNLPIIRMT
                     NVSLRPGLAGSLEDLIADTDNGILLGTNRSWSIDERRLNFQFGTQIGWLIKNGKRAGI
                     VRNPTYTGITPRFWGSCDAICGPDEWEQWGEPYCGKGEPMQTAAVGHGAAPARFRGVQ
                     VGVGRW"
BASE COUNT          923 a         1912 c         1951 g          877 t
ORIGIN      
        1 atcgtgtggc cgtcccggat ctcggcgacc gcgcgacgtg gcgaaacggc tcgcatggcc
       61 tgcctcagat cctcattcgg ccagcggtgg ttgccagcac tgaactcacc tcgtctattg
      121 cgcggtccaa ccgccgtcga cgtagacctc cgagccggtc atgtaggacg ccgcgtccga
      181 ggccaggaac gcgatgacct cggccacttc ctctgaccga cccatgcggc ccatcggggt
      241 gttgttgatg atcagctgct cgatctcggt gccctttacc tgggccacca tcggggtgtc
      301 gatgaatcca gggtgaaccg agttcactcg aatcccttcc ttggcgaagc gaatcgccgc
      361 gttcttggtc atcagtcgaa ccgcaccctt gctggcatgg taggcgatcg atcccccgaa
      421 gccgccgacc gccccgaaga tggagctgag gttgatgatc gagccgcctc ccaccttccg
      481 aaactctgcc accgcggccc gcatcccgag ccacacgccg cgctggttga tggcggtgag
      541 ctgatcccag ccttcgactg tttcctgctc gacatcggcc aacgtaccga tcccggcgtt
      601 gttgaccagg acgtccagcc gaccgtggtc ggcgaccgtt tgcccgatga ccgtggccca
      661 cgccgattca tcggcgacgt cgagatggtg gtacgtgacc gcgcgaccct ttgcggtgag
      721 ctctgacgcg agggcctcgc cctcttcgtc gagaacgtcg gtggcgacaa ccgccgcgcc
      781 gtgctcggcg aggaggcgta ccgtcgccgc tccaatcccg tgggccgcgc ccgtgaccag
      841 tgcgacccgc ccattcagtg acgtgctcat ttcatgtctc ctcttcaggc gggatgggcc
      901 cgcgttcacg gcgatcgtat ggggccctgg ggagtggcct aagggccggc cggaccctct
      961 cgctgtgcct gatggccctg tcgccgttct tgctgagcgc agacgatgac cggtgagccg
     1021 gcttgagtga gcgaggtctc tcgcgggccc tcacaggaag gagccaaatc atggacaaga
     1081 tcctcgtcgc atacgacgga accgagcctg ccgaccgtgc attggccacg gccatcgatc
     1141 tggccaaggc attcgacgcc accgtcggcg tcgtcagcgt ggtcccggtc catccggggc
     1201 ggtcaccgat cgacccttgg gacgacccgg cggtgcacgc cgagcagctg aagcgggcgg
     1261 acatcattct ccgggcagcc ggggtggagg cgaccctcca ccagcaaggc ggcgaccctg
     1321 cgcgggtgat cgagcaggaa gccgaagctg gcggctacga cacgatcgtg atcggaagcc
     1381 gggggcagaa cgccgtcgag cgcgtgctcc agggcagcgt gtcggagcat gttgcgacgc
     1441 acgccacggc aaccgtcgtg atcacgcggt aaaccccgag cagcagtagg gcgggccggt
     1501 cgcggacttc cgcggtcggc cgctccgtgg cggtatcccc cgattgctca ggaggagggc
     1561 gggctcagta ctcgacgggt gcccgcctat cggcaagcac ccgggcgatc tcggtccggt
     1621 accgcgcgcc cgacggatgt cgggccagcc agtacaggaa gggctgatcg gtctccgcga
     1681 tctcttcgat cgtcctgccg gcgtaatggc cgaagtcgat caccttcggg ttgaaggtgg
     1741 aactggtcca tccaggaccg acgggcgtgc tctcgccggc gtcctcgtct tccgggcggt
     1801 acgggtcctg caggtaggtc acggtcccca tcgtggccga atggtacccc cagtcggcaa
     1861 ggtccggatc aaacctgatc aggcgactgg cccggaatct ggtccggcgg cacgcgctgg
     1921 tggagaccca gcgcggcgtt gttccgccat gagacgacgc cgacccagac catgaaaagg
     1981 gccagggcca gcagaacgaa cgggatcccg aagcccggtg ccatgatgtc caccaggtcg
     2041 gcgatgaccg gggcaacgag gaccgggaag aacgaggcca ccgacaggag cgtgttcagg
     2101 atgccgaaaa tccgcccgcg gacgtcgggc ggcagctcct cctggagcga ggtctgggcc
     2161 gggatcgcga tcagggcgta cgcgatgccc gcgccgaatg cgatgacaat gacaagggcg
     2221 atgaccgaca ggagagggcc cagcaggtcg ggcaggtcct cctcgatcgg cgccagggtc
     2281 ggccggagga tatcggtgag aggcccgacc agggcgatgg ccaggagcat ggccgcggtc
     2341 agcatcagcc cgacgtcgat cagcagccgc cgggggatgt actgcccgaa cgagttgatg
     2401 aacaggatgc ccatcacggc gcccagccca gccgggccca tgatgaagaa gatgtcctcc
     2461 gcgctcaggt tgagcacccc ggtggcgtag cccggcccca gggcgccgag cacgccgatg
     2521 atcgacgcgc cgatgaccag gtagcggagc gaccacgaga tgcgcggcgt gttttggacg
     2581 aaggcaatcc cctcgcgcag ctcctcgaga acgtgggtca tcgtcttgcc cgcatcgaac
     2641 ggccgcggcg tcttctccgg cttgacgctg ggcagcggga tgatcgccac cgcggccagg
     2701 gcgaacatga ccgagaccgt gacgtagacc gcgttcgggc cgagggtggt cagcagcaaa
     2761 ggaccgatca ccccgaaccc gatcccgaac gtcgcgttca cggtgaggat gaagatcgag
     2821 ttggcggcca taagatggcg gcgctcgacg aggcgggtga tcgcgatcag ctcggccggg
     2881 ccgaagaacg cgctgatcgt cgagatcgcg aagttcagga ccaggatcgc gaacacgttc
     2941 gcgccgagcg cgatgaacag cgcacagccg aacgcccgtc cgaggttgga caggatcatg
     3001 atccagcgcg catcgctgcg ctcgaccagc acgccggcca gcgcgctgaa gaccacggcg
     3061 gggacgagga aggtcaggat caggagcgcg ttggccgtgt tcgagccggt cgcgccgacc
     3121 accaccgcca tcagcgcggc aaggaccatg ttgctcgcca gctggctcag gacctgggcc
     3181 gaccacagga agacgaagtt ccggttgcgg aagacactgc cggtgcccgc cggcaggccg
     3241 ggctcgacgt cggtcaaatc gatgtgggtc atgacaccga ctccggtccg tgctccaggt
     3301 gtccggtgta gcgccgctcg acgtcgagcc ggtcgagcag gacgcgatgc ccacaacggg
     3361 cgcagacgag gccgatgtcg gcccccagcc gaacgaccga ccactcgtgg ctcccgcatg
     3421 gatgcggctt gcgcagcgtg atccgatccc ccagccgcag ctcgaccggg accgccatca
     3481 gacggtggcc tcgttggctg tgacgcccgt gaagttccac ccgtcgatcc ggatggccgg
     3541 aacgcgactc gcgatgccgg tgtactcggc cccgaccagc cgggtttcgg ccgcgatcgc
     3601 ggagatgcgc gagaacgcgt ccgggatgga ctgggtgaag cggaggttgc ggaccggccc
     3661 cgccaccgcg ccgtcctcga taaggaacgt cccgtcgcgg gtcatcccgg tcaggatccc
     3721 cttcttggga tggacgatgt tcacatagtg gaagcgggtg attagcagcc cacggtcgat
     3781 tccgtccagc aggcggtctc gggccgtgtc gccgggcgcc atgaacagat tccaacacag
     3841 tggaccgaac gtgttcggcg ccggcagccc gtggccggtg cggggtacgc cggcccgata
     3901 ggcggtcgcg ccgtcgtgga cggtctcgcg ggccacgccg tcggtgacga gcgcgacccg
     3961 gcgcttcggc acaccctcga agtcgatcgt cgacgggatg cctgtcgtgt cgagcccgtc
     4021 gtcccagatc gtgacgttcg ggccaaacgc gggctgaccg agctccacgc acgcgcggcc
     4081 ctcttcgagg gcgagaccgg agaacgccat gaacgcgagg tactcgagca cttcggcgac
     4141 cgcatattcg cccagcacga ccggatattc gccggggccg agatctccgg gctccgccgc
     4201 gcgagccgtg cgatcggcgg cttccgaccc gacggcgtgg ggatcgatcg cgcggagatc
     4261 ggtatcgatg gcctgggcgt agcccgtctc ccccgccggg ccgatcatga ccgtcacgag
     4321 cttggcctgg gtcacgccgt tcgagctggc aagaccggtg gagttggcca cggtcatgcg
     4381 ctgggcctcg atcgagtacg cgccgctgac ggtcaggtcg cgggccacgc cggcatcgat
     4441 gaccgcgcgg gcgccttcag cgcgctcccg cggatcagct tccgcggtgg cctccaccca
     4501 gccgaggtcg gagtccgatc ccgtctcgtt ggcgaacggc ggcgggtctg cttctggcgt
     4561 ggcatggcgg gcgatcgcgg cggcccgggc cacgacttcc tcgatcccgg ccgcatcgag
     4621 gcgattcgtg acggcgaccc cggttcgggc gtcgcgccgc acacggaccc gcagagttgc
     4681 gtcgtgctcg gccaggttct gatgaatcat cgagccggcg aatcgggtca gggcgaggtc
     4741 ccgctcggtg acggtggcct ctccctcgct gccgggctcg atccgggcaa gtgcggcctc
     4801 ggccgtttcg cggcctacca tcggccgacc ccgacctgga caccgcggaa gcgggccggt
     4861 gccgcgccgt ggcccaccgc cgcggtctgc atcggctccc ccttgccgca gtacggttcg
     4921 ccccactgct cccactcgtc cggcccgcag atcgcgtcgc atgagcccca gaaccgaggc
     4981 gtgatgccgg tgtaggtcgg gttgcggacg atgcctgctc gcttcccgtt cttgatcagc
     5041 cacccgatct gggtcccgaa ctggaagttc agccggcgct cgtcgatcga ccacgagcga
     5101 ttggtgccga gcaggatccc gttgtcggtg tcggcgatga ggtcctccag cgaccccgcg
     5161 aggccgggcc gcaggctcac gttggtcatc cggatgatcg gcaggttccg ccagctcgcc
     5221 gaccggctac tgcccatcga ctggcggccg atcaccgggg ccgtctctcg cgaggtgagg
     5281 tagccgacga atcggccggc gctgaccagg ggcacgttct ggccgcgcac cccctcgtcg
     5341 tcccagccga acgtgccgag accgccgacg gcggtggcat cggcgtcgat gttgaccacc
     5401 ggcgagccgt actggaatcg atcgaggcga tcggtggtca ggaagctcgt cccggcgaag
     5461 gaggcttcca tgccgaggac ccgatccagc tcggtggggt ggccgcatga ttcgtggacc
     5521 tggatcgcga cctgcgaggc ctcgaggatg acgtccatct cgcccgacgg gcactgcggt
     5581 gcgtcgagca gggcgacggc ctcctccgcg atgcgctgcc catgggcccc caggtccatc
     5641 tcccggatgg cctcgaaacc ggc
//
LOCUS       DASYGU010000061         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_574870, whole genome shotgun sequence.
ACCESSION   DASYGU010000061 DASYGU010000000
VERSION     DASYGU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574870"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGO32_01610"
     CDS             complement(<1..515)
                     /locus_tag="VGO32_01610"
                     /inference="COORDINATES: protein motif:HMM:NF026154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEV7199481.1"
                     /translation="MTFKEFSGKNVEEAIRAAMKEFGADLGDLDIEILAQGSRGILGV
                     GSDEARILAAPKSAVAEASSLVADAPASAPAPPRGRSRAPAPEPQPDVDLAAEMAETA
                     EEASAGQTDETAPTADADLAEAGGLTPVMADEATAPADDRPRSRGRGRDGGSGRGSRE
                     SRPPRSDEARNG"
     gene            complement(512..1642)
                     /locus_tag="VGO32_01615"
     CDS             complement(512..1642)
                     /locus_tag="VGO32_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEV7199482.1"
                     /translation="MEPLFAAGFLGIESFNPPWDIIFLPLFNALIGLYEILFHDLALA
                     IIVLTIVIRTLLAPLFIRQIRSQKEMQRMQPLVKEIQRKHKGNQRKISEETAALYKEH
                     GVNMFGGCLPLVLQLPILFALYQTLTRASSVITFQPSENQQASFTAFLAVSPDVVPLG
                     NNMYQVPIDGSCVVPQDFHQFLPLNCQLITPFKLDAPVDTIVEWLTNPFTGVSLNLAT
                     VDHAFAIYLPIGAGFAISMLAVLAALLQFIQVRMTSPRGGAGDDPTATTTQTMTYLFP
                     IMTIVWGGIFPSGLVLYWGVYTAYLIIQQYLILGWGNLFPLFGWQPRWAPSPDAGDLA
                     ATKPKSPTPPPTNGSGSTPEPARPGGQRRSGRRRRGREGRKR"
     gene            complement(1652..1858)
                     /gene="yidD"
                     /locus_tag="VGO32_01620"
     CDS             complement(1652..1858)
                     /gene="yidD"
                     /locus_tag="VGO32_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV7199483.1"
                     /translation="MRLIGMGLIRAYQALTFWMPPTCRYTPSCSAYTYQAIDRYGLFR
                     GSWMGAKRIGRCHPFHPGGYDPVR"
     gene            complement(1863..2201)
                     /gene="rnpA"
                     /locus_tag="VGO32_01625"
     CDS             complement(1863..2201)
                     /gene="rnpA"
                     /locus_tag="VGO32_01625"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV7199484.1"
                     /translation="MPAYPTLRRRADFEAVVRSGSLSATRLLVLRARRTDGPITRIGL
                     ATPASLGGAVERNRVRRRVRALVRARHRQMGAGWDLLVIMKPDARSATYDELGAALDA
                     ALSHAGVSAE"
     gene            complement(2219..2353)
                     /gene="rpmH"
                     /locus_tag="VGO32_01630"
     CDS             complement(2219..2353)
                     /gene="rpmH"
                     /locus_tag="VGO32_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV7199485.1"
                     /translation="MKRTYQPKKRARKRNHGFRARMASKGGRRVLARRRAKGRSRLSA
                     "
     gene            2646..>3259
                     /locus_tag="VGO32_01635"
     CDS             2646..>3259
                     /locus_tag="VGO32_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEV7199486.1"
                     /translation="MDAKQVWRAVLGELQVSLSAANFETWLKDTSLEEIDEHLFRISA
                     PNGFTRDWLDNRYRTLVSQTLARVVGRSVRVEFVVADAPGPAPEQASDELTPVPASTP
                     SGNAVNLNARYTFGNFIVGSANRLAHAAALSVAERPGHAYNPLFLYGGVGLGKTHLMH
                     AIGHAVAGKFPRKRILYATSEKFTNDFINSIREQKMEDFRSRYRR"
BASE COUNT          506 a         1076 c         1149 g          528 t
ORIGIN      
        1 ccgttgcgcg cctcgtcgga gcggggtggg cggctctcgc gtgatccgcg gcccgagcca
       61 ccatcacgcc cgcggccacg gctgcgcggt cggtcgtcgg cgggagccgt ggcctcgtcg
      121 gccatgaccg gggtcagacc gccggcctcg gccaggtcgg cgtccgcggt cggtgccgtt
      181 tcatcggtct ggcctgccga ggcctcctcg gccgtctcgg ccatctcggc cgcgaggtcg
      241 acgtcgggct gcggctcggg agccggagcc cgggatcgtc cgcgaggggg cgcgggcgcg
      301 gatgccggag cgtcggccac caggctcgaa gcctcggcca ccgccgactt gggagcggcc
      361 aggatgcggg cctcatcgct gcccacgccc aggatgccgc ggctcccctg cgccaggatc
      421 tcgatgtcga ggtcgcccag gtccgcgccg aactctttca tcgcggcgcg aatggcttcc
      481 tccacgttct ttccggagaa ttccttgaag gtcatcgctt acggccctcc cggcctcgtc
      541 gtcgccgacc ggagcgtcgc tgtccgccgg gccgggctgg ctccggggtg ctgccgcttc
      601 cattggtcgg aggtggtgtt gggctcttcg gcttcgtggc cgcgagatcg ccggcgtcag
      661 ggctcggcgc ccatctcggc tgccagccga agagcgggaa tagattcccc cagcccagga
      721 tcaggtactg ctggatgatc aggtaggcgg tgtacacacc ccagtacagg acgagtccgc
      781 tggggaagat gccgccccac acgatggtca tgatcgggaa caggtaggtc atcgtctggg
      841 tggtggtggc ggtcggatca tcgccggccc cgccgcgcgg cgaggtcatc cgaacctgga
      901 tgaactggag cagcgcggca aggaccgcga gcatggagat cgcgaagccg gctccgatgg
      961 gcagatagat cgcgaacgca tggtcgacgg tcgccaggtt gagcgacaca ccggtgaacg
     1021 ggttggtcag ccactcgacg atggtgtcga cgggggcgtc gagcttgaac ggggtgatga
     1081 gctggcagtt caggggcagg aactggtgga agtcctgggg gaccacacac gaaccgtcga
     1141 tcgggacctg gtacatgttg ttgccaaggg gcacgacgtc ggggctcact gccaggaatg
     1201 cggtgaagct cgcctgctgg ttctcactcg gctggaaggt gatgacgctc gaggcccggg
     1261 tcagggtctg gtacagggcg aacaggatgg gaagctggag cacgagcggc aggcagccgc
     1321 cgaacatgtt gaccccgtgc tccttgtaga gcgccgcggt ttcctcgctg atcttccgct
     1381 ggttgccctt gtgcttgcgc tggatctcct tgaccagcgg ctgcatccgc tgcatctctt
     1441 tctgggagcg gatctggcga atgaagagcg gggcgaggag cgtccggatg acgatggtca
     1501 ggacgatgat ggccagcgcc aggtcgtgga acaggatctc gtacagcccg atgagggcgt
     1561 tgaacagggg gaggaagatg atgtcccagg gcgggttgaa ggactcgatc cccaggaatc
     1621 cggcggcgaa cagtggctcc acggcgcgtt cctagcggac cgggtcgtag ccgccggggt
     1681 ggaacggatg gcagcggccg atccgcttgg cgcccatcca tgatccgcgg aacagcccgt
     1741 accggtcgat ggcctggtag gtgtaggccg agcacgacgg ggtgtagcgg caggtgggcg
     1801 gcatccagaa cgtgagcgcc tggtaggcgc gaatgagacc catgccgatc aggcgcacgc
     1861 ggtcactccg cgctgactcc ggcatgcgac agcgccgcgt cgagggcggc tcccagctcg
     1921 tcgtatgtcg cgctccgcgc gtccggtttc atgatcacca gcaggtccca gcccgctccc
     1981 atctgtcggt gacgcgctcg gaccagtgca cgcacgcggc gtcgtacccg atttcgttcc
     2041 acggcccctc ccagggaggc cggggtcgcg agaccgatcc gggtgatcgg tccgtcagtt
     2101 cgtcgagccc gcaggacgag caaccgagtg gcgctgaggc tcccggagcg gaccacggcc
     2161 tcaaagtcgg cccggcgacg gagcgtcgga taggccggca ttgccgtggg ccccgaggtc
     2221 aggcgctcag ccggctgcgc cccttcgccc ggcggcgggc cagcacacga cgaccgccct
     2281 tggaagccat gcgagcgcga aagccatgat tccgctttcg ggcgcgcttc ttgggctggt
     2341 aggtccgttt catcgtcgtg ggctttccct ttgttcagcc agagcggcgg ccggtcgccg
     2401 aacatgcgcg aggctggctt ttcgccagcc tcatagacgt cataggcggg tcgggcgtcg
     2461 cgtggcggcg attctaaccc acccggccaa gaatcgttgc cgggccgatg gcactggcgt
     2521 acactacgcc gacccccgga gccagacgcc cagcccacgg gccgtgcccg ccccggagga
     2581 gcaccagatt aaggatttcg cccggttcgt ggctcccgcc atgccggtct ggaggcgtct
     2641 gtcgaatgga tgcgaaacag gtgtggcgcg ccgtgctcgg cgagctgcag gtcagcctct
     2701 cggccgcgaa cttcgaaacg tggctgaagg acacctccct cgaggagatc gacgagcacc
     2761 tgttccgcat ctcggccccg aacggcttca cccgtgactg gctggacaac cgctaccgga
     2821 ccctggtcag ccagaccctc gctcgcgtcg tcggccgatc ggtccgcgtc gagttcgtcg
     2881 tcgccgacgc ccccggcccc gccccggagc aggccagcga cgagctgacc cccgtgccgg
     2941 cctccacgcc gagcggcaac gcggtcaacc tgaacgcccg ctacacgttc gggaacttca
     3001 tcgtcggcag cgccaaccgc ctggcccacg ccgccgccct gtcggtggcc gagcggccgg
     3061 gtcatgccta caacccgctg ttcctgtacg gcggggtggg cctgggcaag acccacctca
     3121 tgcacgccat cggtcatgcg gtggccggca agttcccgcg caagcggatc ctgtacgcga
     3181 ccagcgagaa gttcacgaac gacttcatca actccatccg cgagcagaag atggaggact
     3241 tccggagccg atatcggcg
//
LOCUS       DASYGU010000062         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1552107, whole genome shotgun sequence.
ACCESSION   DASYGU010000062 DASYGU010000000
VERSION     DASYGU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552107"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VGO32_01640"
     CDS             complement(<1..961)
                     /locus_tag="VGO32_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199487.1"
                     /translation="MTNPVGAHLNGGLAVADAETAMREIATRIGDRVNRIPDGETGER
                     NNWIFFQVDRLRSLPGVETVGVFDPTRGYDGLPKLRITDQSVVAEFFESFNPGYADAY
                     LASYGTFTSLRGDGTIPGNVRFQVQYPTPFATAAGWFVPEQVPAVLPAYTAAMLRDLN
                     RLLAEIPPEDIAVQWDVAVEFGALTGSFPGFPDLTSAEAGRDLGRLLDAVPQDIPAGL
                     HLCYGDYQHVHFKQPESLALQIELIGHVAANARRTVNWFAITVPQNETRPEFFAPLAD
                     LRIKDGTEVYFGIVPYHVAEQPEGTSAAQIALIDAHVASWRPHS"
     regulatory      complement(969..1059)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1130..1205
                     /locus_tag="VGO32_01645"
     tRNA            1130..1205
                     /locus_tag="VGO32_01645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1163..1165,aa:Gly,seq:tcc)
     gene            1222..2367
                     /locus_tag="VGO32_01650"
     CDS             1222..2367
                     /locus_tag="VGO32_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7199488.1"
                     /translation="MTRLRAAVIGTGFVGPHHVDAIRRTGYADVAVLVGSNPDRTARR
                     AADLGVDHWSIDTDAVLADSTIDVVHICTPNHTHVSLGQAVLEAGKHLVLEKPIAVDR
                     AGAEALVEAAARSNRHAMVALTYRGYPMVQQARSMVAEGHLGEIRLVRGGYIQDWLAD
                     AADYNWRVDPEVGGRSRAIADIGTHWFDTAEFVTGLRVKTVQADLATFIPTRSRSLDG
                     GTAFSTARGPAEEVAVASEDAATMLLRFKGGARGACVVSQVSTGNKNAFSLHVDGSHG
                     SLTWEQELPERLTVRERSEERLYVRDPGGQQASAGVPWLPAGHPEGWGEALRDLLRPF
                     YAAIAGGSPPVGDGAPYPTLTVGARSIAFVEAALASSTAGAWTAIEA"
BASE COUNT          394 a          807 c          918 g          396 t
ORIGIN      
        1 cggagtgcgg ccgccacgag gcgacgtgcg cgtcgatgag ggcaatctgg gcggcactgg
       61 tgccctcggg ctgctccgcc acgtggtacg gcacgatccc gaagtagacc tccgtcccgt
      121 ccttgatccg caggtcggca agtggagcga agaactcggg tcgcgtctcg ttctgcggga
      181 cggtgatcgc gaaccagttg acggtgcgcc gggcgttggc ggcgacgtgg ccgatgagct
      241 cgatctggag agcgaggctc tcgggctgct tgaagtggac gtgctggtag tcgccatagc
      301 aaaggtggag gccggcaggg atgtcctgtg gcacggcgtc gagcagccga ccaagatcgc
      361 gacctgcctc cgcagacgtc aggtccggaa agccggggaa cgagccggtc agcgcgccga
      421 attcgacggc gacgtcccac tgcacggcga tgtcctcggg tgggatctcc gccagcaggc
      481 ggttgaggtc acggagcata gccgccgtgt atgccggcag gacggccggg acctgctcgg
      541 gcacgaacca accggcggcg gtcgcaaagg gtgttgggta ctggacctgg aagcggacgt
      601 tcccggggat ggtgccatct ccgcggaggg aggtgaaggt tccgtagctc gcgaggtagg
      661 catcggcgta gccgggattg aatgactcga agaactcggc aacgacggac tggtcggtta
      721 tgcggagctt gggcaggccg tcgtatcccc gcgtgggatc gaaaacgcct accgtctcga
      781 cgccgggcag cgatcggagc cgatcgacct ggaagaagat ccagttgttg cgctccccag
      841 tctcaccgtc ggggatgcga ttgacccggt cgccgatccg tgtggcgatc tcgcgcatgg
      901 cggtttcggc atcggcgacg gccaatccgc cgttgaggtg cgcgcctacg gggttggtca
      961 tgacagcact cccatcgttc ctcgcagggg caccgctccc atagggtcgg ttgccgggcc
     1021 gtcaacgagc gaggtctctc aggccactct ggataggtgt cacgcgtacg tgacgggccc
     1081 atggtacccg ctggccccac agaacggggc gattacaatc ggcgagccgg cgggagtagc
     1141 tcagttggca gagcgtcagc cttccaagct gaatgtcgcg ggttcgatcc ccgtctcccg
     1201 ctccacctct acgatcacga ggtgacccgc ctccgcgccg ccgtgatcgg gaccggcttt
     1261 gtcggtccgc atcatgtcga tgccatccgg cgaaccgggt acgcggatgt tgcggtcctg
     1321 gtcgggtcga acccggatcg gacagcgcgg cgcgccgcgg atctcggagt ggaccattgg
     1381 tcgatcgata cggatgccgt cctggccgac tcgacgatcg atgtcgtcca catctgcacc
     1441 ccgaaccaca cccatgtctc gctcgggcag gcggtgctcg aggccggcaa gcatcttgtc
     1501 ctcgagaagc cgatcgctgt ggatcgagca ggagccgagg cgctggtcga ggccgcagct
     1561 cggtcgaacc ggcatgcgat ggtggcgctc acctaccgcg gctatccgat ggtccagcag
     1621 gcccggtcga tggtcgccga aggccacctt ggcgaaatcc ggctcgtccg cgggggatac
     1681 atccaggact ggctggcgga cgcggccgac tacaactggc gcgtggatcc ggaggttggc
     1741 ggtcgatccc gggcgatcgc tgacatcggg acgcactggt tcgacaccgc cgagttcgtg
     1801 accggcctcc gggtgaagac cgtgcaggcc gacctggcga ccttcatccc aacccgttcg
     1861 cggagcctcg acggtggcac ggccttcagc acggcgcggg gtccggcgga ggaggtggcg
     1921 gtggcttccg aggatgccgc caccatgctg ctgcgcttca agggcggcgc cagaggagcc
     1981 tgcgtcgtga gccaggtctc gaccgggaac aagaacgcgt tctcgctcca cgttgacggc
     2041 tcacacgggt cgctgacctg ggagcaggag ctgcccgagc gcctcaccgt tcgggagcgc
     2101 agcgaggaac ggctctacgt ccgtgacccg ggcgggcagc aggcgagcgc cggcgtcccg
     2161 tggctcccgg ccggccaccc ggaggggtgg ggcgaggcgc tgcgcgacct tctccggccg
     2221 ttctacgcgg ccatcgccgg cgggagtccg ccagtggggg acggggcgcc ctacccgacc
     2281 ctcacggtcg gcgcacggag catcgccttc gtcgaggccg cgctggcgtc gtcaacggct
     2341 ggtgcttgga ccgccatcga ggcctgagcc atgacggaga aggctcccga ggtccgggtc
     2401 agctgcgcgg ccgagcccgg cggctgggcc tgcacggtcc gggtgggcgg cgatccggcc
     2461 accacgaccc accatgtgcg agtcgcgacc gaggacctgg agcggctggg ccggc
//
LOCUS       DASYGU010000063         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_523639, whole genome shotgun sequence.
ACCESSION   DASYGU010000063 DASYGU010000000
VERSION     DASYGU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523639"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VGO32_01655"
     CDS             <1..405
                     /locus_tag="VGO32_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV7199489.1"
                     /translation="VPDQDPREIEQMMIDHLRTIAPPTVTVTVRSLSNGAPAITPLDH
                     PAVSAASRASEKAFGKAPIFHRSGGSIPYLPALERHTGIKSVMLGWSSPNGNFHAPNE
                     WMPMANYRGGLDCIVYLWDEIGKMTAAEMRGD"
     gene            619..1623
                     /locus_tag="VGO32_01660"
     CDS             619..1623
                     /locus_tag="VGO32_01660"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV7199490.1"
                     /translation="MPPPLAPPLVEAAPTGPVFGGFWIRFAAYIIDGLILGLISIVID
                     VVLFNLISSASTLQSVGSVIGLVIGFLYFAWSWVRTGSTPGMSLLGLQVVRDADGGPI
                     TWGKAFIRYVGIIISTLVLFIGLIWVAFDKRKRGWHDLMAGTVVIRKPEVPITGGRKV
                     LMIGAIGCGCLLPILAIIGIVGLIFMGPIVQDIFNRVGDDLARASSGEIVEPVPISFE
                     SLRVGDCFNIVNPDDPATVGLQAVPCADEHVFEVFFAEDMPAGAYPGEEAVTTFANDN
                     CLPAFEEYVGVSFDESVWYASPLGPTEVDWDDGIQRVTCQLHNQEGTPVTGSARGSAE
                     "
     gene            complement(1639..>2199)
                     /locus_tag="VGO32_01665"
     CDS             complement(1639..>2199)
                     /locus_tag="VGO32_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV7199491.1"
                     /translation="RELLRSAGHDTADWAIVASGPNAASPHHTPGERIITEGDAIVLD
                     IGGTANGYSSDTTRTAFVGVPPEEFQDLFAVLQEAQAAACDAVRPGIAARDLDRVARE
                     IITDAGYGPLFNHRTGHGIGLETHEEPYLVESNPEPLREGDAFSIEPGIYVSDRWGAR
                     IEDIVVCTADGGDRLNRSSRDLRVVA"
BASE COUNT          336 a          748 c          738 g          377 t
ORIGIN      
        1 gtgccggacc aggatcctcg cgagatcgag cagatgatga tcgaccacct gcggacgatc
       61 gccccgccga cggtcacggt caccgtccgg agcctctcga acggcgcgcc ggccatcacc
      121 ccgctcgacc acccggccgt cagcgccgcg tcacgggcct cggagaaggc gttcggcaaa
      181 gcgccgatct tccatcggtc cggcggcagc attccgtacc tgcccgccct cgagcggcac
      241 accggcatca agagcgtaat gctgggctgg tcgtcaccga acgggaactt ccacgccccc
      301 aacgagtgga tgccgatggc gaactaccgt ggcggcctcg actgcatcgt ctacctgtgg
      361 gacgagatcg ggaagatgac cgctgccgag atgcgcggcg actgaccgga gattctgctg
      421 ggcatttccg cacccgaggc ttagggtgtt ttgatgaacg agccgcccaa ctcgcccccg
      481 aagccgtctg aggccgagcc agaacccgac gccgatccca cggagtccgc gccggccgcc
      541 ccggatcagg tgagcggaga agagggcgct ccgccagcga cgaccgaggc tgaatcggtg
      601 ccgcatacgg ctgaacccgt gccgccgccg ttggcgccgc cactggtcga ggcggccccc
      661 acgggtcccg tgttcggcgg attctggatc cggttcgccg cgtacatcat cgacggcctg
      721 atcctgggac tcatatcgat cgtgatcgac gtggtgctct tcaacctcat ttcctccgcg
      781 agcaccctcc agtccgttgg atccgtgatc ggactcgtga tcgggttcct gtacttcgcg
      841 tggtcctggg ttcgaactgg tagcacgccg gggatgagcc tgcttggcct tcaggtggtt
      901 cgcgacgccg acggcggccc gatcacctgg gggaaggcat tcatccggta tgtcggcatc
      961 atcatctcga cccttgtcct gttcatcggt ctgatctggg tcgcgttcga caagcgcaag
     1021 cggggatggc acgacctcat ggccgggacc gtcgtgatcc gcaagcccga ggtgccgatc
     1081 accggcggcc gcaaagtgct catgatcggg gccatcggtt gcggctgcct gctgccgatc
     1141 ctggccatca tcggcatcgt gggcctcatc ttcatggggc cgatcgtgca ggacatcttt
     1201 aaccgcgttg gcgatgacct tgcccgcgcg tccagcgggg agatcgtcga gccggtcccg
     1261 atcagcttcg agtcgctccg ggtgggcgac tgcttcaaca tcgtcaatcc ggacgaccca
     1321 gccacggtcg ggctgcaggc ggtcccgtgc gccgacgagc acgtgttcga ggtgttcttc
     1381 gccgaggaca tgccagccgg cgcgtatccg ggcgaagagg ccgtgaccac gttcgcgaac
     1441 gacaactgtc tcccggcctt cgaggagtac gtcggcgtgt ccttcgacga gtctgtctgg
     1501 tacgcctcgc cgctcgggcc gaccgaggtg gactgggatg acggcattca gcgggtcacg
     1561 tgccagctcc acaaccagga ggggacgccg gtcaccggct ccgctcgcgg ctcggccgag
     1621 tagccctgtg cgagtggtct aggccaccac ccggaggtcg cggctcgagc ggttgaggcg
     1681 atcgccgccg tccgcggtgc agaccacgat gtcctcgatc cgggcgcccc agcggtctga
     1741 gacgtagatc ccgggctcga tgctgaacgc gtccccctcg cggagcggct cggggttcga
     1801 ctcgaccagg tatggctcct cgtgggtctc gagaccgatc ccgtgaccgg tccggtggtt
     1861 gaacagcggc ccgtagccgg cgtcggtgat gatctcgcgc gcaacgcggt ccaggtcgcg
     1921 agcggcgatc cccggccgca ccgcgtcgca ggcggcggcc tgggcctcct gcaggacagc
     1981 aaacaggtcc tggaactcct ccggcggcac cccaacgaac gcggtccggg tcgtgtcgct
     2041 cgaatagccg ttggccgtcc cgccgatgtc cagcacgatg gcgtcgccct cggtgatgat
     2101 ccgctcgccc ggcgtgtgat gcgggctggc ggcgttgggt ccggacgcga cgatcgccca
     2161 gtccgcggtg tcatgaccgg ctgatcggag cagctcgcg
//
LOCUS       DASYGU010000064         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_472170, whole genome shotgun sequence.
ACCESSION   DASYGU010000064 DASYGU010000000
VERSION     DASYGU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472170"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1261
                     /locus_tag="VGO32_01670"
     CDS             77..1261
                     /locus_tag="VGO32_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7199492.1"
                     /translation="MDHLAEVLFQLFVLLAATKIGDELFKRIGQPPLVGEILAGVIVG
                     PALLGWYQITPETELFAQIGAVLLLFEVGVHTRVGDLVRVGGTAVTVAVLGVVLPFIG
                     GFAVATAMGFSIEGRVFLAAALTATSVGITSRALDQLGALKTGSGRIVLGAAVIDDVL
                     AMLIVAVAVGMASGGVDAGDILLTFGLAAAFIVIVLVAGTGILSRRRSLLTEPRFAET
                     PLLPGMILMLGLAALSAAIGLAAIIGAFLAGMVVGESSEREALEKETAPIAALFTPFF
                     FGFVGAQIDITVFGTPSTVALLLGITALAIVTKFAGAFLGALRVGRERALLIGWAMVP
                     RGEVEIIVAGLALAAGAFGPDLYLVVVGMVVITSLVVPPFMPRLVRRAEPGIGIAQAA
                     AE"
     gene            complement(1330..>1608)
                     /locus_tag="VGO32_01675"
     CDS             complement(1330..>1608)
                     /locus_tag="VGO32_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7199493.1"
                     /translation="DRGSIWEAGHLAWMMNEINKLVWPAEAGIGHLDEGELTQTVDTA
                     SAAGILTGPPDDAAFNSAIWEEALSGIDGDTTGDDFAAPEVPITPGGE"
BASE COUNT          186 a          539 c          572 g          311 t
ORIGIN      
        1 gcccgtatcg ccggtccaag gtggagactg cgcaaaccgg tgcgcagttt ttctgtattc
       61 ggacgaggtt gagtccgtgg atcacctggc cgaggtcctg ttccagctct tcgtcctgct
      121 ggccgcgacc aagatcggcg acgagctgtt caagcgcatc ggccagccgc cgctggtcgg
      181 ggagatcctg gccggcgtga tcgtcggccc cgcgctgctg ggctggtacc agatcacgcc
      241 cgagaccgag ctgttcgcgc agattggcgc cgtcctgctg ctgttcgagg ttggcgtcca
      301 cacccgggtc ggagacctgg tgcgggtcgg cgggacggcc gtgacggtcg ccgtgctggg
      361 tgtcgtcctg ccgttcatcg gtggcttcgc ggtggcgacc gccatgggct tctcgatcga
      421 gggccgcgtg ttcctggccg ccgccctgac cgcgacctcg gtgggcatca cctcgcgggc
      481 gctggaccag ctcggggcgc tgaagacggg ctcgggcagg atcgtgctgg gtgcggcggt
      541 catcgacgac gtgttggcca tgctgatcgt ggccgttgcg gtggggatgg cgagcggcgg
      601 cgtggacgcc ggcgacatcc tgctgacgtt cgggctggcg gccgcgttca tcgtgatcgt
      661 cctggtggcg ggtaccggca tcctgtcccg acgtcgctcg ctcctgaccg agccgcgctt
      721 cgcggagacc ccgctcctcc cgggcatgat cctcatgctc ggtctggccg cgctgagcgc
      781 agccatcggc ctggcggcga tcatcggtgc cttcctggcc gggatggtgg tcggcgagtc
      841 gtcggagcgg gaagcgctcg agaaggagac ggcgcccatc gccgcgctct tcaccccgtt
      901 cttcttcggc ttcgtgggtg cccagatcga catcacggtg tttggaaccc catcgaccgt
      961 cgccctcctg ctgggcatca ccgcgctcgc gatcgtcacc aagttcgccg gcgcattcct
     1021 cggggcgttg cgggttggcc gcgaacgggc cctgctcatc ggctgggcca tggtcccgcg
     1081 cggtgaggtg gagatcatcg ttgccgggct ggccctggcg gccggcgcat tcggtccgga
     1141 cctctacctg gtggtggtgg ggatggtggt catcacgtcc ctggtggtcc cgcccttcat
     1201 gccgcggctc gtgcgccggg ccgagccggg gatcggtatc gcgcaggcgg ccgcggaata
     1261 gcaggaggcc cctcgcggac gaggggcctc catcggcttc agattgtggg gcttcatccc
     1321 actcgtcggt tactcgccgc cgggcgtaat cgggacctcc ggcgcggcaa agtcatcgcc
     1381 ggtcgtatca ccgtcgatcc ccgaaagcgc ctcttcccag atggcgctgt tgaacgccgc
     1441 gtcgtccggt ggtccggtca ggatcccggc cgcgctggcc gtatccacgg tctgggtgag
     1501 ctctccctca tccaggtggc cgatgccggc ctcggccggc cagaccagct tgttgatctc
     1561 gttcatcatc caggccaggt ggcccgcttc ccagatgctg ccccgatc
//
LOCUS       DASYGU010000065         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_369952, whole genome shotgun sequence.
ACCESSION   DASYGU010000065 DASYGU010000000
VERSION     DASYGU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369952"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VGO32_01680"
     CDS             complement(<1..658)
                     /locus_tag="VGO32_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7199494.1"
                     /translation="MTVVRGEPLIIAPDRLPASVLELVHRTVERRPNKEALRWKSGSG
                     WSSWTYSQLWEQIAATSIGLRGMGLAAGDRVVILSRSRPEWVVADLACMALGAVTCPI
                     YPADPPARMARIVREVDARFVIVEDERLLQRLRSGFEDGELPGSVVLFDSNESAGSRS
                     LAAVAGEGATGSARGAWDRMWRSARPDQVATIVHTIGTDGIPLGVVVRHGNLVHSFHA
                     I"
     gene            complement(655..>1681)
                     /locus_tag="VGO32_01685"
     CDS             complement(655..>1681)
                     /locus_tag="VGO32_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV7199495.1"
                     /translation="YLFGMPLMFFLAGAATWLAVERRGIGGHTGLRLRRLLIPLVLGL
                     IIFGPPQAWIAYVAAGGEAGPIEFLGTYVANLNPTLNPRWFGDVGHHLWFLGFLFLYV
                     LVTLPLLSWLRTRRAAGAETFIGRVASARGGLIWLAVPIAAVQLVLRPLFPDYRDWAD
                     FALWLGYFVIGIGAMADPRVMPAILGRRRATLWLAPVVAFAYLPVVLFGSPIDIEHAP
                     GFTLAGMAYIIWRTALGWVMTLVFVGVAATYFTARPRFLGWASAMVLPFYVIHHPVTV
                     LVAALVVPLDLGLYLKYGLIVLVAGTVTVALCVALDLATAALAGRLGRGPSTRVSVSE
                     PSAEVQP"
BASE COUNT          311 a          588 c          588 g          194 t
ORIGIN      
        1 cgatggcgtg gaacgagtgg accaggtttc cgtggcgcac caccacgccg agcgggatcc
       61 catcggttcc gatggtgtgc acgatggtcg ccacctggtc gggccgggct gatcgccaca
      121 tacggtccca cgccccgcgg gcagatccgg tggcgccctc gccggcgacc gccgccaggc
      181 tgcgcgatcc cgccgactca ttcgagtcga acagcaccac cgatcccggc agttccccat
      241 cctcgaaccc ggagcgcaga cgctggagga gccgttcgtc ctcgacgatc acgaaccggg
      301 cgtcgacctc gcggacgatc cgcgccatgc gggccggcgg gtcggcgggg tagatcgggc
      361 aggtgacggc cccgagcgcc atgcaggcga ggtcggcgac gacccactcc gggcgcgatc
      421 ggctcaggat gacgacccgg tcccccgcag caagacccat cccgcggagc ccgatcgacg
      481 tggctgcgat ctgctcccat agctgggagt aggtccagct cgaccagcca gagccgctct
      541 tccatcggag ggcctccttg ttcgggcggc gctccaccgt gcggtgcacg agctccagca
      601 cgctggccgg cagccgatcg ggcgcgatga tcagaggctc tccgcgcacc acggtcatgg
      661 ctgcacctcg gccgaaggct cggacaccga tacccgggtc gatggtccac ggcccagcct
      721 cccggcgagc gcggcggtgg ccaggtccag cgccacgcac agcgcgacgg tgactgtgcc
      781 ggccacgagc acgatcaggc cgtacttcag gtacaggccc agatcgagtg ggacgaccag
      841 ggcggctacc aggacagtga ccgggtggtg aatgacgtag aagggcagca ccatcgcgct
      901 cgcccagccc aggaatcgcg ggcgcgcggt gaagtacgtg gcggccacgc ccacgaagac
      961 gagggtcatg acccagccga gcgcggtccg ccagatgatg tacgccatcc cggccagcgt
     1021 gaatcccggg gcatgctcga tgtcgatcgg cgacccgaac aggacgaccg ggagataggc
     1081 aaaggccacg accggagcca gccacagggt cgcccgtcgg cggccgagga tggcaggcat
     1141 gacccgcggg tcggccatcg cgccgatccc gatcacgaaa tagccgagcc acagcgcgaa
     1201 atcggcccag tcccggtagt cggggaacag cggtcggagc accagctgaa cggcggcgat
     1261 gggcaccgcg agccagatca ggccgccgcg ggcgctggcc acgcgaccga tgaacgtctc
     1321 ggccccggcc gcccgtcgag tccggagcca cgacaggagc ggcagcgtca cgaggacgta
     1381 gaggaacagg aagccaagga accacaggtg atggcccacg tcgccgaacc agcgcggatt
     1441 cagggtggga ttcaggttcg cgacgtacgt gcccaggaac tcgatcggcc ccgcctcccc
     1501 gcccgcggcg acgtacgcga tccaggcctg cggcggacca aagatgatca ggcccaggac
     1561 gagcgggatg agcagccgcc gaaggcggag cccggtgtgg ccgccgattc ctcgccgctc
     1621 gacggctagc caggtcgccg caccggccag gaagaacatg agcggcatcc cgaacaggta
     1681 g
//
LOCUS       DASYGU010000066         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_781354, whole genome shotgun sequence.
ACCESSION   DASYGU010000066 DASYGU010000000
VERSION     DASYGU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781354"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1891)
                     /locus_tag="VGO32_01690"
     CDS             complement(92..1891)
                     /locus_tag="VGO32_01690"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV7199496.1"
                     /translation="MSRFTTLAAVAVAMTFLLVVVGAIVRATDSGLGCPEWPTCHGSW
                     IPPFDDPHAIIEWSHRTVAALTGAVVALLALVAVVRHRDWPALVVGSVAAAGLVVFQA
                     WLGKITVETDLSGEIVTAHLATAMALFALVLFVWVRSRYPRQLSASGGWSPFTILAAL
                     AALATYGLMLLGSHVTATGTALVFDDWPFMDGGLLPTLDPGVLPHVLHRWATIAVGLL
                     VAGVVVAARRGREPAPLPAMATTAGVLYVVQIAVGGAQVLTGLAPWTQVLHVALAAGI
                     FGLLTALVMTADYVGRLPATSTVEGEPRAQARSDVLRAYVALTKPRIIELLLVTTVPT
                     MVLAQGGIPPIPLIGAVLIGGTLAAGGANAINQYLDRDIDQRMRRTRNRPLPRHAVEP
                     TAALVFGLVLSVVSFAWLAVTVNLLSAILAASAIAFYAVVYTAVLKRSTPQNIVIGGA
                     AGCVPVLVAWAAVTNTVALPAIVLFAVIFVWTPPHFWALALRYQSDYEAAGVPMLPVV
                     SGEVETARQILLYSLVLVVVSLVLLPAAGMGLVYLVSAAGLGVGFVVWALRVKRSPGA
                     GPAAIGLFRYSTLYLTLLFAAVAADTLVRAAIG"
     gene            complement(1891..2778)
                     /locus_tag="VGO32_01695"
     CDS             complement(1891..2778)
                     /locus_tag="VGO32_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEV7199497.1"
                     /translation="MSATTSRSSLTDRLPRGGLFAPGSVSWRVDRESVVLAGGTCALL
                     LQIAHPAVAAGVDAHSDFRADPFARLRRTLGASWSIVFGDRAAAERAIRRINAIHQKV
                     GGVVPETGRPYRALDPALLMWVHATLVDTALRMYDRFVGPLDATEMDVYHREAAEVAI
                     RLGVPEAILPGSVAEMRAWMDGLIAAGEVQVGPTALALLPAILYPTRFPPHSVWDVAH
                     LASISVLHPAIRRQYGLHWNRRRERGMVRLAAASRRILPLLPPAVRYVPPARAGERAR
                     LSWPRTGAIRPSSADLSAE"
     gene            complement(2744..3205)
                     /locus_tag="VGO32_01700"
     CDS             complement(2744..3205)
                     /locus_tag="VGO32_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV7199498.1"
                     /translation="MADLSPSGVVEGIHLHGDRGEPMRVVADAELVAGHGLVGDRMAG
                     LGIPGTHVTFIGAEGVEAMVAETGIPLAPHQTRRNILVRGVDVPGLVGRRFQVGDAVC
                     YGVKECHPCIHLESVTRAGVREGLSGRGGLRADVLAGGQIRVGDHITILPD"
BASE COUNT          566 a         1174 c         1245 g          383 t
ORIGIN      
        1 gctcgcgagg aggcgcgaca ggacgccgag gcggcccagc aggccgcgct cgattcgggc
       61 tccggctgac cccctcggct ctggactgac ttcagccgat ggcggcgcgg accagcgtgt
      121 cggccgcgac cgcggcgaac aggagggtca ggtacagggt tgagtagcgg aacaggccga
      181 tggccgcggg cccggctccg ggtgagcgct tgacccgcag cgcccagacg acgaagccca
      241 cgcccaggcc ggcggcggaa acgaggtaga cgaggcccat cccggcggcg ggcagcagga
      301 ccaggctgac cacgaccagc accagcgagt acagcaggat ctggcgggcc gtctccacct
      361 cgccgctgac caccggcagc atcggaaccc cggctgcttc gtagtcggac tggtagcgca
      421 gcgcgagtgc ccagaaatgg ggcggggtcc agacgaagat cacggcgaac aggacgatcg
      481 cgggcagtgc caccgtgttg gtaaccgccg cccatgcgac gagcacgggc acgcaaccgg
      541 cggcgccgcc gatgacgatg ttctggggcg tggaccgttt caggacggcg gtatagacga
      601 ccgcgtagaa ggcgatcgcg ctggcggcca ggatcgcgct gagcaggttg acggtgaccg
      661 ccagccacgc gaacgacacc acactgagga cgaggccgaa gacgagggcg gcggtcggct
      721 cgaccgcgtg gcgcggcagg ggtcggttcc gcgttcgccg catccgctgg tcgatgtcgc
      781 ggtcgaggta ctggttgatc gcgtttgcgc cgccggcggc cagcgtcccg ccgatcaaca
      841 ccgcaccgat caacgggatg ggcgggatcc cgccctgggc caggaccatg gtggggactg
      901 tggtcacgag caggagttcg atgatccgcg gcttggtcag cgccacgtag gcacgcagaa
      961 cgtcactccg agcctgcgcc cggggctcgc cctcgacagt ggaggtggcc ggcagtcgac
     1021 ccacgtagtc ggcagtcatg accaacgcgg tcagcagccc gaagatcccg gccgccagcg
     1081 cgacatggag gacctgggtc cacggcgcga gaccggtgag gacctgtgcg ccaccgaccg
     1141 cgatctggac gacgtacagc accccggcgg tggtggccat cgccggcaac ggggccggct
     1201 cgcggccgcg gcgagcggcc acgaccacgc ctgcgaccag cagcccgacc gcgatcgtcg
     1261 cccagcggtg caggacgtgg ggcaggacac cgggatccag ggtcgggagg aggccgccgt
     1321 ccatgaacgg ccagtcgtcg aagacgagcg ccgtcccggt cgcggtcacg tgcgacccga
     1381 gcagcatgag gccgtaggtc gcgagcgccg cgagcgcggc gaggatggtg aacgggctcc
     1441 atccgcccga cgcggacagc tggcgcgggt accggctgcg gacccagacg aagagcacga
     1501 gcgcgaagag cgccatggca gtcgccaggt gggcggtcac gatctccccg gacaggtcgg
     1561 tctcgaccgt aatcttgccc agccacgcct ggaagacgac caggcccgcg gcggccacgc
     1621 tgccgaccac cagggccggc caatcacgat gccggacgac ggcgaccagg gcgaggaggg
     1681 cgaccacggc cccggtgagg gcggccacgg tccggtggct ccactcgatg atcgcgtggg
     1741 gatcgtcgaa tggcgggatc cacgacccgt ggcaggtcgg ccattccggg cagccgagcc
     1801 cggaatcggt ggcgcgcaca atggcgccga ccaccaccag caggaatgtc atggccacgg
     1861 ccaccgcggc gagggtcgtg aatcgggaca tcactcagcg ctgagatcgg cgctcgatgg
     1921 gcggatggcg ccggtgcgcg gccatgatag acgggcccgc tcgccggccc gcgcgggcgg
     1981 cacataccga accgcgggcg gcaggagagg caggatccgg cggctggcgg cggcgagccg
     2041 gaccatgccc cgctcccggc gccggttcca gtgcaggccg tactgccgcc ggatggccgg
     2101 gtggaggacc gagatcgagg ccaggtgcgc cacgtcccat accgagtgtg gcgggaatcg
     2161 ggtcgggtac aggatcgcgg gcagcagggc gagggcggtt ggcccgacct ggacctcacc
     2221 ggccgcgatc aggccgtcca tccaggctcg catctcggcc actgagcccg gcaggatggc
     2281 ctccggcacg ccgagccgga tcgctacctc ggccgcctcg cggtggtaga cgtccatctc
     2341 ggtcgcgtcg agcgggccca cgaaccggtc atacatgcgc agggcggtgt cgaccagggt
     2401 ggcgtggacc cacatcagca gggccgggtc caaggcgcgg tacggtcgcc ccgtctcggg
     2461 caccaccccg ccgaccttct ggtggatggc attgatccgg cggatggccc gctccgccgc
     2521 cgcgcggtca ccgaacacga tcgaccagct cgcgcccagc gtccgccgca gccgggcgaa
     2581 tggatcggcc cgaaagtccg aatgcgcgtc gacgccggcc gccaccgccg ggtgggcgat
     2641 ctggaggagc agggcacagg tcccgccggc gagcaccacg ctctcgcggt ccacccgcca
     2701 cgagaccgaa ccgggcgcga acagcccgcc ccggggcagc cgatcagtca gggaggatcg
     2761 tgatgtggtc gccgacacga atctgccccc cggccaggac atcggcccgg agtccgcccc
     2821 gaccggacag cccttcgcgg acgccggccc gggtcaccga ctcgagatgg atacaggggt
     2881 ggcactcctt gaccccgtag cacaccgcgt ccccgacctg gaaccgacgg ccgacgaggc
     2941 cgggcacgtc cacgccccgg accaggatgt tgcgtcgggt ctgatgcggc gcgagcggga
     3001 tgcccgtctc ggcgaccatg gcctccaccc cttcggcgcc gatgaaggtc acgtgggtgc
     3061 cgggaatgcc gagcccggcc atccggtcgc ccaccagccc gtgccccgcc accagctcgg
     3121 catcggccac gacgcgcatc ggctcgcccc ggtcgccgtg gaggtggatg ccctccacca
     3181 cgccgctggg gctcaggtca gccacggcaa ggtgatgggc agaaggatgt tcaggagaag
     3241 cccgaggatg agcatcgcgc ccgcccgacg ggtgtggatg aacgccccgc ccacgaacca
     3301 cagcggccag ccgacgtacg cgttgggcgg ggtcacgggc ttgggtacgg cgaactggcg
     3361 gagaacct
//
LOCUS       DASYGU010000067         4632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1604596, whole genome shotgun sequence.
ACCESSION   DASYGU010000067 DASYGU010000000
VERSION     DASYGU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604596"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..934)
                     /locus_tag="VGO32_01705"
     CDS             complement(143..934)
                     /locus_tag="VGO32_01705"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEV7199499.1"
                     /translation="MTIAASPSGTTPAVVPPPDLDSRSASLPAPVARWLRHARPNLSI
                     VPQTIYLEGAARFKRGRLPYLPLDIRIWTRLGFDRVSELEVRIAGLTLMRGLDAFVDG
                     HGFTRVGSELSTGPSVDQGAFHVMFLETLLVPGAWPADIRWEPVDADSARVITPFVGG
                     LEKARVTFDLRTGLPAAYSTDRYRTAGEGKLRWTATVADWHDFGTLFYPRLVEARWAD
                     QPQPWLTMRIERASIDVPMDEHLERARRVLRERVEGSSPGVSRFS"
     gene            complement(1034..1420)
                     /gene="rplL"
                     /locus_tag="VGO32_01710"
     CDS             complement(1034..1420)
                     /gene="rplL"
                     /locus_tag="VGO32_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV7199500.1"
                     /translation="MSKDQILEAIDGMTVLELSELVKAFEERYGVTAAAPVAAAAAPA
                     AGGGDGAGPAASEEQTEFDAVLTDVGPNKILVIKAVRELTGLGLKEAKDLVDAAPKAV
                     KEGVARDEADAVKEKLAEAGASVEVK"
     gene            complement(1477..2235)
                     /gene="rplJ"
                     /locus_tag="VGO32_01715"
     CDS             complement(1477..2235)
                     /gene="rplJ"
                     /locus_tag="VGO32_01715"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV7199501.1"
                     /translation="MPTDAKRQAVSELTEMLRGSSALAVADYRGLTVSEMHTVRRSLR
                     SNGVSLKIAKNRLLKIAADEAGVAELKTLLDGPTAIAATSGDEVSLARALQDAFRPYK
                     MVTIRGGLLSGQPVSASDLQRLASLPSREVLLARLAGGMAAPLTGMAGVLAGNLRNLI
                     GVLNAVVDAKRATDVGVAGDAAATEASPPEAAESAAESDSADVAPTEAIATEDAASTA
                     DESPPEESPVAESPAEESSAEADSATDDDPPPTE"
     gene            complement(2429..3136)
                     /gene="rplA"
                     /locus_tag="VGO32_01720"
     CDS             complement(2429..3136)
                     /gene="rplA"
                     /locus_tag="VGO32_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV7199502.1"
                     /translation="MPAHGKKYRAAAALLEEGRRYPIEEACELLPRVSTAKFDATVEA
                     HLRLGIDPRHADQLVRGTVVLPHGTGRSTRVIVFAQGEKAQDALRAGADEVGGEELVK
                     KIDDGWFDFEVAVATPDMMGVVGRLGKKLGPRGLMPNPKAGTVTFDIERAVGEVKSGR
                     IEFKVDRAGIVHAPVGRASFSPDQLQANVAALVDAINRAKPSGAKGTYLRTLTLAPTM
                     GPGVAVDIPSALAAAQA"
     gene            complement(3136..3561)
                     /gene="rplK"
                     /locus_tag="VGO32_01725"
     CDS             complement(3136..3561)
                     /gene="rplK"
                     /locus_tag="VGO32_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV7199503.1"
                     /translation="MAKKVRAILRVQGQAGKATAAAPVGPALGQHGINILEFLKAFNE
                     KTAHLSGYTVAAEITVYEDRSFTFTTMAPLAAALLKRAAGVESGSAEPNREKVGSVTQ
                     AQLRELAEMKMSDLNANSPEQAMKIIAGTARSMGIEVRD"
     gene            complement(3607..4179)
                     /gene="nusG"
                     /locus_tag="VGO32_01730"
     CDS             complement(3607..4179)
                     /gene="nusG"
                     /locus_tag="VGO32_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV7199504.1"
                     /translation="MSREAPQPEDPDAAERTTKQWYVIHTYSGYENKVKTNLEHRIES
                     MGVEDQIYQVLVPMEEEIEIRNGQRQTVNKKVFPGYVLVEMIMTDESWYVVRNTPGVT
                     SFVGSGNRPTALREGEVKAILKQMGVEAPKFKVQFQKGQSVRVTDGPFAEFIGTVDEV
                     HPERNKVRVLVSIFGRETPVELDFLQIEKL"
     gene            complement(4446..>4632)
                     /gene="secE"
                     /locus_tag="VGO32_01735"
     CDS             complement(4446..>4632)
                     /gene="secE"
                     /locus_tag="VGO32_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV7199505.1"
                     /translation="IARTRNYLTESWAELRKVAWPTRRTVVNLTLIVIAVSALVGAYI
                     AILDAILFFGMDQLLTR"
BASE COUNT          649 a         1568 c         1608 g          807 t
ORIGIN      
        1 cacggctcgg agcgagcgcc gagcggccgg cggagtcggc caggggcatg cagaactcct
       61 cgaaggggca gagtggaccg atgcggaagc agcgtgggtc gcggacacgc gaaagaggag
      121 tatagcagtc ggcgccgctt cgtcaactga agcgggatac gccgggcgat gatccttcca
      181 ccctctcgcg cagcacccgt cgcgcccgct cgaggtgctc gtccatggga acgtcgatgc
      241 tcgcgcgctc gatccgcatc gtgagccacg gctgcggctg gtcagcccac cgggcctcga
      301 ccagcctcgg gtagaacaac gttccgaagt cgtgccaatc ggccaccgtc gcggtccacc
      361 gcaacttccc ctcccccgcc gtccggtagc gatcggtgct gtacgcggct ggcagaccgg
      421 tcctgaggtc gaaggtcacc cgggccttct cgagaccgcc cacgaacggg gttatcacgc
      481 gcgccgaatc ggcatcgacc ggctcccacc gaatgtcggc tggccaggcg ccgggcacga
      541 gcagggtttc caggaacatg acgtggaagg ccccctgatc gaccgatggg ccggtgctca
      601 gctcggatcc aacccgcgtg aagccgtgac catccacgaa tgcatcgagg ccccgcatga
      661 gggtgaggcc ggcgatgcga acctcgagct cgctgacgcg gtcgaagcca agtcgagtcc
      721 agatccgaat gtcgagcgga aggtagggca ggcgcccgcg cttaaagcgc gccgctccct
      781 ccagatagat ggtctgtggc acgatcgaca ggttcggacg ggcgtgacgc aaccatcggg
      841 ccacgggtgc cggcagcgaa gctgaacgcg aatcgagatc aggcgggggc accacggcgg
      901 gtgtggtccc gctgggtgaa gcggcgattg tcatcggatg gtctcccgtc ggcagctatc
      961 gaccggagca tcctggaatc gaaacgcccg ggccagtgcc gtacggcacc cgcccgcggg
     1021 cgcatcggca gggctacttg acctcgacgc tcgcgccggc ctccgccagc ttctccttga
     1081 cggcgtccgc ctcgtcgcga gcgacccctt ccttcaccgc cttgggggcg gcatcgacca
     1141 ggtccttggc ctccttgagg cccaggccgg tcagctcgcg gacggccttg atgacgagga
     1201 tcttgttcgg gccgacgtcg gtcaggaccg cgtcgaactc ggtctgctcc tcggaggcgg
     1261 ccgggccggc gccgtcgccg ccaccggcgg ccggggcagc ggcagccgca accggggcgg
     1321 cggcagtcac gccgtagcgc tcctcgaagg cctttaccag ctcgctcagc tcgaggacgg
     1381 tcatcccgtc aatcgcctcg aggatctggt ctttgctcat tgtcttggtg gccatggtgg
     1441 tatcggttct ccttcttcgg tcagctcagg tgctggtcat tcggttggcg gtgggtcgtc
     1501 gtcagtggcg gaatccgcct cagcggagct ctcctcggcg ggactctccg cgacaggact
     1561 ctcctcaggg ggcgattcgt ccgcggtcga ggctgcgtct tcggtggcaa tcgcctccgt
     1621 gggggccacg tcggcggagt ccgattcggc ggccgattcc gcggcctcgg ggggactcgc
     1681 ctcggtcgcg gcggcgtcac cggcaacacc aacatcggtc gcgcgcttgg cgtcgacgac
     1741 ggcgttcagg acgcctatga ggttgcgcag gttgccggcc aggacgccgg ccattccggt
     1801 caggggcgcc gccatgcctc cggccagtcg ggcgaggagc acctcgcggc tgggcagact
     1861 ggcgagtcgc tggaggtcgg atgcgctcac cggctggccg gagagcagtc cgccgcggat
     1921 ggtcaccatc ttgtacggac gaaacgcgtc ctggagcgca cgggccaggc tgacctcgtc
     1981 gccggaggtt gcggcgatgg ccgtcggccc gtcgaggagc gtcttgagct cggccacccc
     2041 ggcctcgtcg gccgcgatct tcaggagccg gttcttggcg atcttcaggc tcacgccatt
     2101 cgagcgcagc gaccgtcgaa cggtgtgcat ctcggagacg gtcagtccgc ggtagtcggc
     2161 cacggccagg gccgaactgc cacggagcat ctcggtgagc tcgctcaccg cctggcgctt
     2221 ggcatcggtt ggcatgtgct ggtgctccac ctacgaaaga gtcctcgccg cggcgaggac
     2281 cagacccact cggtgggtgg agcgtcctgc ctcggcgtgc cccggccggt gcctcaccgg
     2341 ccggtgatta gtccgggccg gagcccggat cacatcgctg tctggggcta ttgagttgtg
     2401 ctcccgggcg tgcccgctcc cccgtcggtc aggcctgggc ggcggcgagg gccgatggaa
     2461 tatcgaccgc gacgccgggt cccatggtgg gggctagggt caaagtccgc aagtacgtgc
     2521 ccttggcacc ggacggcttg gcgcggttga tggcgtccac cagggccgcg acgttggcct
     2581 gcagctgatc cggcgagaac gaggcgcggc ccaccggggc gtggacgatc cccgcccggt
     2641 cgaccttgaa ctcgatgcgg cccgacttca cctcgcccac ggcgcgctcg atatcgaacg
     2701 tgacggtccc cgccttcggg ttgggcatca ggccccgtgg gccgagcttc ttcccgaggc
     2761 ggccgacgac gcccatcatg tccggggtcg cgacggcgac ctcgaagtcg aaccagccat
     2821 cgtcgatctt cttgaccagc tcttcgccgc cgacctcgtc cgcgccggca cggagcgcgt
     2881 cctgcgcctt ctcgccctgg gcaaagacga tgacccgggt cgagcggccg gtgccgtgtg
     2941 gcaggaccac ggtgccgcgg accagctggt ccgcgtggcg tgggtcgatc ccgagccgga
     3001 ggtgcgcctc caccgtggcg tcgaacttcg cggtcgacac cctgggcagc agctcgcagg
     3061 cctcctcgat cggatagcgg cggccctcct ccaggagtgc ggccgcggcg cggtacttct
     3121 tcccgtgggc aggcatcagt cgcgcacctc gatacccatg cttcgcgcgg tgccggcaat
     3181 gatcttcatc gcctgctcgg ggctgttcgc gttcagatcg ctcatcttca tctcggccag
     3241 ctcgcggagc tgggcctgcg tgacggagcc gaccttctcc cggttgggct cggccgaccc
     3301 gctctccacg ccggcggctc gcttcagcag cgcggcagcg agcggggcca tggtcgtgaa
     3361 cgtgaacgac cgatcctcgt agacggtgat ctcggccgcg acggtgtagc ccgacaggtg
     3421 ggcggtcttc tcgttgaagg ccttcaggaa ctcgaggatg ttgatgccat gctggcccag
     3481 ggccgggccc accggggcgg cggcggttgc cttgccggcc tggccctgga ccctgaggat
     3541 ggcgcgtact ttcttggcca cgggtattcc tccgaggtac gagcggggcg ggttgcgccc
     3601 ctccctctac aacttctcga tctgcaggaa gtcgagctcc acgggcgtct cgcgcccgaa
     3661 gatgctgacc agaacccgga ccttgttccg ctccgggtgc acttcgtcca cggttccgat
     3721 gaactcggcg aacggcccgt cggtgacgcg cacgctctgg cccttctgga actggacctt
     3781 gaacttgggc gcttcgacgc ccatctgctt gaggatggcc ttgacctcgc cctcgcggag
     3841 cgcggtcggc cggttgcccg agcccacgaa gctcgtgacg ccgggcgtgt tgcgcacgac
     3901 ataccacgac tcgtcggtca tgatcatctc gaccagcacg tagccgggga agaccttctt
     3961 gttgacggtc tgccgctggc cgttgcggat ctcgatctcc tcctccatgg gcacgaggac
     4021 ctggtagatc tggtcctcga cacccatcga ttcgatgcgg tgctcgaggt tggtcttcac
     4081 cttgttctcg tagcccgagt aggtgtgaat cacgtaccac tgcttggtgg tccgctccgc
     4141 ggcgtcggga tcctccggct ggggcgcctc gcgggacagg cgcgcggcct ggcgggcctc
     4201 cgcgatctcg accggtgtcg gggcgggctc cgccgctgcg ggctcgaccg cagcctcggc
     4261 cggggcgggc tcctcagcag ccggttcggc ttcgacagcc tccatctcga cggtgtcctc
     4321 ggcctgcgca gcctcgttgg tcggctcgtc cgtctcgtcg ggaagcaacg cctcgatggg
     4381 ctcgatgggt tcggcggtct cggtcgtatc ggtctggtcg gtggtcattg gttcggtctc
     4441 ggatgtcatc gggtgaggag ctggtccatg ccaaagaaca ggatcgcgtc caggatcgcg
     4501 atgtaggccc cgaccagcgc ggacaccgcg atcacgatca gggtcaggtt cacgaccgta
     4561 cggcgggtgg gccacgcgac cttgcgcagc tcggcccagc tttcggtcag gtagttgcgg
     4621 gtcctggcga tc
//
LOCUS       DASYGU010000068         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_987537, whole genome shotgun sequence.
ACCESSION   DASYGU010000068 DASYGU010000000
VERSION     DASYGU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987537"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGO32_01740"
     CDS             <1..311
                     /locus_tag="VGO32_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199506.1"
                     /translation="TALGLLPAILYPTRFPPRSVWDMAHLASTSVLHPTIRRQYGLIW
                     NRRREQGVGRLAAASRRIVPVLPPAVRYVPPARAVQLPRLSWPRTGAIRPSSADLSAE
                     "
     gene            311..>1773
                     /locus_tag="VGO32_01745"
     CDS             311..>1773
                     /locus_tag="VGO32_01745"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV7199507.1"
                     /translation="MSRFTTLAAVAVAMTFLLVVVGAIVRATESGLGCPEWPTCHGSW
                     IPPFDDPHAIIEWSHRTVAALTGAVVALLALVAILRHRDRPALVIGSVAAAGLVVFQA
                     WLGKITVETDLSGEIVTAHLATAMALFALVLFVGVRSRYPRQLSASGGWSPFTILAAL
                     AALATYGLMLLGSHVTATGTALVFDDWPFMDGGLLPTLDPGVLPHVLHRWATIAVGLL
                     VAGVVVTARRGREPAPLPAMATTAGILYVVQIAVGGAQVLTGLAPWTQVAHVALAAGI
                     FGLLTALAVTAHYLGRLAATSVAEGEPRAQARTDVLRAYVALTKPRIIELLLVTTVPT
                     MVLAQGGIPPIPLIGAVLIGGTLAAGGANAINQYLDRDIDERMRRTRNRPLPRHAVEP
                     TAALVFGLVLSVVSFAWLTVTVNLLSAILAASAIVFYAVVYTAVLKRSTPQNIVIGGA
                     AGCVPVLVAWAAVTNTVALPAIVLFAVIFVWTPPHFWA"
BASE COUNT          202 a          679 c          585 g          307 t
ORIGIN      
        1 ccacggcgct tggcctgctg cccgcaatcc tgtacccgac ccgattcccg ccacgctcgg
       61 tgtgggacat ggcgcacttg gcctcgacgt cggtcctgca cccgaccatc cggcgccagt
      121 acggcctgat ctggaaccgg cgtcgcgagc agggggtggg ccggctggct gccgccagtc
      181 gtcggatcgt gcccgttctg ccgcccgcgg ttcggtatgt gccgccggcc cgggccgtcc
      241 agctgccccg tctatcatgg ccgcgcaccg gcgccatccg cccatcgagc gccgatctca
      301 gcgctgagtg atgtcccgat tcacgaccct cgccgcggtc gccgtggcca tgaccttcct
      361 gctggtggtg gtcggcgcca tcgtgcgcgc caccgagtca gggcttggct gcccggaatg
      421 gccgacctgc cacgggtcgt ggatcccgcc atttgacgat ccccacgcga tcatcgagtg
      481 gagccaccgg accgtggccg ccctcaccgg tgccgtggtc gccctcctcg ccctcgtggc
      541 catcctccgc catcgtgatc ggccggccct ggtcatcggt agcgtggccg ccgccggcct
      601 ggtcgtcttc caggcgtggc tgggcaagat cacggtcgag accgaccttt ccggcgagat
      661 cgtgaccgcc catctggcga ccgccatggc gcttttcgcg ctcgtgctct tcgtgggggt
      721 ccgcagccga tacccgcgcc agctgtccgc gtcgggtgga tggagcccct tcacgatcct
      781 cgcggcgctc gcggcgctcg cgacctacgg cctcatgctg ctcgggtcgc acgtgaccgc
      841 gaccgggacg gcgctcgtct tcgacgactg gccgttcatg gacggcggcc tcctcccgac
      901 cctggatccc ggcgtcctgc cccacgtcct gcaccgctgg gcgacgatcg cggtcggcct
      961 gctggtcgcc ggcgtggtgg tgaccgcccg tcgcggccgg gagccggccc cgctgccggc
     1021 catggccacc actgccggga tcctgtacgt ggtccagatc gcggtcggcg gcgcacaggt
     1081 cctcaccggt ctcgcgccat ggacccaggt cgcgcacgtc gcgctggcgg ccggcatctt
     1141 cgggttgctg accgcgctgg ccgtgaccgc gcactacctg ggccggctgg cggccacgtc
     1201 cgtcgcggag ggcgagccgc gggcacaggc ccggacagat gtcctgcgcg cctacgtggc
     1261 gctgaccaag ccgcggatca tcgagctcct gctcgtgacc acggtcccga ccatggtcct
     1321 ggcccagggc gggatcccgc ccatcccgtt gatcggcgcg gtgctgatcg gcgggacgct
     1381 ggctgccggt ggcgcgaacg cgatcaacca gtacctcgat cgggatatcg acgagcggat
     1441 gcgtcgaacc cgcaatcgac ccctgccgcg tcacgccgtg gagccgaccg ccgccctcgt
     1501 cttcggcctc gtcctcagcg tcgtgtcgtt cgcctggctg accgtcaccg tcaacctgct
     1561 cagcgcgatc ctggccgcca gcgcgatcgt gttctatgcg gtcgtctaca ccgccgtcct
     1621 gaagcggtcc acgccccaga acatcgtcat cggcggcgcc gccggatgcg tgcccgtgct
     1681 cgtcgcatgg gccgcggtca ccaacacggt tgcgctgccc gcgatcgtcc tgttcgcggt
     1741 gatcttcgtc tggaccccac cccatttctg ggc
//
LOCUS       DASYGU010000069         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1708409, whole genome shotgun sequence.
ACCESSION   DASYGU010000069 DASYGU010000000
VERSION     DASYGU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708409"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VGO32_01750"
     CDS             complement(<1..990)
                     /locus_tag="VGO32_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199508.1"
                     /translation="MSGNLKRLRPYDRPRKSRTSVLPAGGDGAVMGYLGFGALWLLTA
                     TGLGTFAALQLVMSSFEFTLALPFGYSFELTPDTVSAGFRHAFVWGWLTNAALGAIFF
                     VTPRITGRPLSHTRIGVVGMVLWNIGFGAGLTLLYIPQVAETGTLTGFPVIVNLVLIL
                     ALVLVNVTFWTSVAGSAALFVGVAWFGLALFALAGMTFEAMVVSVLNLDPAINAVLDA
                     MWTRGIALFWLLAVPIGALHYLIPRLTGQPLASGTLGWFGLGSWAVLGVVSLFGAAVS
                     PSLPYALVSFGNAATIMLLVPATAIIGNLTMTLRGRWSLVLSPGPMATAVASLV"
     gene            1093..1647
                     /locus_tag="VGO32_01755"
     CDS             1093..1647
                     /locus_tag="VGO32_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV7199509.1"
                     /translation="MTDPASARDTLRPLRRARQVREFTDEPLTDDELTAITDVARWTG
                     SSRNTQPWRFLVIRAPATIVRLESVGLPQTRSLKTATAVLAIVLSDDEERRISHAYDD
                     GRVAERILIAAGMLDLAAGIAWVRPDVLSAVREILGLPEDLMVRTLMALGHPTAEARK
                     PKSAPGEARLPRDEVVFEERLPDD"
     gene            complement(1663..1995)
                     /locus_tag="VGO32_01760"
     CDS             complement(1663..1995)
                     /locus_tag="VGO32_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEV7199510.1"
                     /translation="MAYDKDLAERLRARLAGLPAVAEREMFGGIAFLVGGNMAVGVTG
                     DELMVRVGKDAHDATVSLPGARIFDLSSSRPMIGWLVVAPEGFATEAEFDSWVQRGVD
                     FAESLPPK"
     gene            complement(2062..2136)
                     /locus_tag="VGO32_01765"
     tRNA            complement(2062..2136)
                     /locus_tag="VGO32_01765"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2102..2104),aa:Met,seq:cat)
     gene            complement(2181..2507)
                     /locus_tag="VGO32_01770"
     CDS             complement(2181..2507)
                     /locus_tag="VGO32_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEV7199511.1"
                     /translation="MNIDEMLQGARDTMTVKRVYGEPIEKEGLTLIPAAEVMGGGGGG
                     SDEANNGGGGFGVSARPVGMWIIRGDEVEWEPSVDATKMALRGMLVAIIGLLVFRSVV
                     KTVVRR"
     gene            complement(2529..>2739)
                     /locus_tag="VGO32_01775"
     CDS             complement(2529..>2739)
                     /locus_tag="VGO32_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199512.1"
                     /translation="WTRYGVQVVGWQTGFNPVVAMELLDRGAWAGTGILGPEAFDPDP
                     YLAMLDRDGIHWGMHEMEPGAHRPT"
BASE COUNT          482 a          973 c          898 g          386 t
ORIGIN      
        1 cacgagtgac gccacggccg tcgccatcgg gcccggcgac aggacgaggc tccatcggcc
       61 gcggagggtc atggtcaggt tgccgatgat cgcggtcgcg ggcaccagga gcatgatcgt
      121 ggccgcgttg ccgaagctta cgagcgcgta cgggagcgac gggctgaccg ccgctccgaa
      181 caacgacacg acgccgagca ccgcccagga gccgagcccg aaccagccaa gtgttccgct
      241 ggccaacggt tggcccgtga gtcgggggat gaggtagtgg agcgccccga tcgggaccgc
      301 gaggagccag aagagggcga tgccgcgagt ccacatcgca tcaagcacgg cgttgatggc
      361 agggtccagg ttgagcacgg atacgaccat cgcctcaaag gtcattccgg ccagcgcgaa
      421 caacgcgagc ccgaaccagg ccacgcccac gaagagcgcc gctgagcccg cgacgctcgt
      481 ccagaacgtg acgttgacca ggaccagggc caggatcagg acgagattga cgatcaccgg
      541 gaagccagtg agcgtcccgg tctcggcgac ctgggggatg tagagcaggg tcaggccggc
      601 cccgaacccg atgttccaga ggaccatgcc caccaccccg atccgggtgt gggacagcgg
      661 tcgaccggtg attcgcgggg tgacgaagaa gatcgcgccc agggccgcgt tcgtgagcca
      721 accccacacg aacgcgtgcc ggaagccggc agagacggtg tccggcgtca gctcgaagct
      781 gtagccgaag gggagggcga gggtgaactc gaagctcgac atcaccagct ggagggcggc
      841 gaaggtgccg agcccggtgg cggtcaggag ccagagcgcc ccgaaaccga ggtatcccat
      901 caccgcgcca tcacccccag cggggagcac tgacgtgcgg gacttccgcg gccggtcgta
      961 ggggcggagc cgcttcaggt ttccggacat ccgagcgcgc gaacctccag gctgagaccc
     1021 cgatcggggc ggttccgcac aactgggcca cgcatcgcga cccggcggca ttgtaacggg
     1081 cacaatcggc ccgtgaccga tccggcctcc gcccgcgaca ccctccgtcc gctccgccgc
     1141 gcccgccagg tccgagagtt cacggacgag cccctgaccg atgacgagct gaccgcgatc
     1201 accgacgtcg cccgctggac cggcagcagc cgcaacaccc agccgtggcg cttcctggtc
     1261 atccgtgccc cggctacgat cgtccgcttg gagtcggtcg ggctccccca gacccgttca
     1321 ctgaagaccg ccacggcggt gctcgccatc gtgctttccg acgacgagga acgccggatc
     1381 tcgcatgcat acgacgacgg tcgagtcgcg gaacggattc tcatcgcggc gggcatgctc
     1441 gatctcgcgg ccgggatcgc atgggtccgg cccgacgttc tttccgccgt ccgcgagatc
     1501 ctgggcctgc ccgaggatct aatggtccga accctgatgg ccctcggcca cccgacggcc
     1561 gaggcacgga aacccaaatc tgccccgggc gaagcccgac tgccccggga cgaggtcgtc
     1621 ttcgaggaac gcctgcccga cgactgagcg cagtcagtgc ggctacttcg gcggcaggct
     1681 ctccgcgaaa tccacgccgc gctggaccca ggaatcgaac tcggcctcgg tcgcgaaccc
     1741 ctcgggcgcg accaccagcc agccgatcat cgggcggctg gacgacaggt cgaagatccg
     1801 ggccccgggc agcgagaccg tcgcgtcgtg agcgtccttg cccacccgga ccatgagctc
     1861 gtcgccggtc acgcccaccg ccatgttgcc gcccacgagg aacgcaatcc cgccgaacat
     1921 ctcccgctcg gcaaccgccg gcagcccggc gagccgggct cgaagccgct ccgcgagatc
     1981 cttgtcatac gccacgtcga atgctccgga aaaggcgaaa gccgcccggt cggaggcggc
     2041 cttcgcgcgt ggtaagcgga ttggttgcgg gggacagatt cgaactgccg accttcgggt
     2101 tatgagcccg acgagctacc gctgctccac cccgcgactt cctagggtac cggtcggttg
     2161 gcgatcggtc aatcgtctga tcagcggcgg acgacggtct tcaccaccga gcgaaagacg
     2221 agcaggccga tgatcgccac cagcatcccg cgcagtgcca tcttggtcgc gtcgaccgat
     2281 ggctcccact cgacctcgtc gccgcggatg atccacatcc cgaccggccg ggcgctgacc
     2341 ccgaagccgc cgcctccgtt gttcgcctcg tcgctcccgc ccccgccccc gcccatgacc
     2401 tcggcagcag gaatgagggt caggccttcc ttctcgatcg gctcgccgta gacgcgcttc
     2461 acggtcatcg tgtcgcgggc gccctgcagc atttcgtcaa tgttcatcgg tctgttctcc
     2521 tcgccggttc aggtgggccg gtgggctccg ggctccatct cgtgcatacc ccagtggatg
     2581 ccatcacggt caagcatggc cagatatggg tcggggtcaa atgcctccgg tccgaggatc
     2641 ccggtaccgg cccacgcgcc gcggtccagg agctccatgg cgacgaccgg gttgaacccg
     2701 gtttgccacc cgacgacctg caccccatag cgggtccat
//
LOCUS       DASYGU010000070         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_628075, whole genome shotgun sequence.
ACCESSION   DASYGU010000070 DASYGU010000000
VERSION     DASYGU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628075"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..360
                     /locus_tag="VGO32_01780"
     CDS             169..360
                     /locus_tag="VGO32_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HEV7199513.1"
                     /translation="MARVCEICGKRPITGWNAQSVGMNRKRAGRRWIPNLQTLTTVRD
                     GRTVTVRACSRCRRTATKA"
     gene            complement(357..812)
                     /gene="dtd"
                     /locus_tag="VGO32_01785"
     CDS             complement(357..812)
                     /gene="dtd"
                     /locus_tag="VGO32_01785"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV7199514.1"
                     /translation="MRLVVQRVSGASVRTGSRELGSIGRGAVVLVGIAPTDDDEIVDR
                     MATKLIGLRYFTDAEGRTNLALGDIGGALLVVSQFTLLADVRHGRRPGFTDAAPPEVA
                     APLVDRFVATLRAAGITVETGEFGALMDVGLVNEGPFTLVVDSAESLSG"
     gene            complement(817..>2010)
                     /locus_tag="VGO32_01790"
     CDS             complement(817..>2010)
                     /locus_tag="VGO32_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEV7199515.1"
                     /translation="GVQTGTDGTYALDGVPTEGTLIYKAPGYQLGVIPVDEEMTKDVT
                     LPPFVARALYAPSAVFEAAGRLDAMLDLIDRTEVNAMVIDVKETGGWLYYSTDLPEAV
                     SAGALMSTPVFDLEELLPALKERGIYTIARVVVMKDNTLGQSRPELAVRNTSTGQPWT
                     DWGGGTWLDPGNPGVAEYIAAIAGDLAAKGFDEVQLDYIRFFSDGPYELAETNLPHTQ
                     SFRLPTIQRVMRVISSELAWTRTFLGADVFPIAFIATDDQGIGQRPEVIMPYVDYFSP
                     MVYPSHYYPGVFDIAEPNEHPYEMIDHTLEIMNAEAAGLPLRIRPWIQDFGYAEFRAY
                     TAADVQAEKQAVADNGGAGWMIWNASAIFTEAALGGPLEGEAPVPVTAADPPAAAPAS
                     PSGSL"
BASE COUNT          301 a          692 c          702 g          315 t
ORIGIN      
        1 gtcgaggcgc tccggagaca cggccgggat cggtccccca ccccggcgcc cggcggtcgc
       61 cgcgcgccgc gggcctgatt gcatgcctcg atcgtagcgt gctagcatcg ccaccggtcc
      121 ggctgcccgc agggtgggcg ccggcttctt cattgaatgg agtcctgaat ggcccgcgtc
      181 tgcgagatct gcggcaagcg cccgatcacc ggctggaacg cgcaatcggt cggcatgaac
      241 cgaaagcgag ctggtcgacg ctggattccg aaccttcaga cgctgacgac ggtccgcgac
      301 ggacgcacgg tcaccgtgcg cgcctgctcg cgctgccggc gcaccgccac caaggcctag
      361 ccggacaggg actcggccga gtccacgacc agggtgaacg gcccctcgtt caccagcccg
      421 acgtccatca gggcaccgaa ctcgcctgtc tcgaccgtga tccccgctgc ccgcagggtg
      481 gccacgaacc ggtcgacgag cggcgcggcg acctctggtg gtgccgcatc ggtgaacccc
      541 ggacgccggc cgtggcggac gtcggcgagc agcgtgaact ggctgacgac gagcagcgcg
      601 ccaccgatgt cgccaagcgc gaggttcgtc cggccctcgg catcggtgaa gtagcgcagg
      661 ccgatcagct tggtggccat ccggtccacg atttcgtcgt catcggttgg cgcgatcccg
      721 accagcacga cggcgccgcg tccgatggac cccagctcac ggctcccggt gcggacgctg
      781 gccccggaca cgcgctggac gaccaggcgc atgggtctag agtgagccgg atgggctggc
      841 tggcgcggca gccggtggat cggccgccgt gaccgggacg ggcgcctccc cctcgagcgg
      901 tccgcccagc gcggcctcgg tgaagatcgc gctcgcgttc cagatcatcc atcccgcgcc
      961 cccgttgtcg gccacggcct gcttctcggc ctgcacgtcg gccgcggtgt aggcccggaa
     1021 ctcggcgtag ccgaagtcct ggatccaggg ccggatgcgg agcgggaggc ccgcggcctc
     1081 ggcgttcatg atctcgaggg tgtggtcgat catctcgtac gggtgctcgt tgggctcggc
     1141 gatgtcgaac acgccggggt agtagtggct cgggtagacc atcgggctga agtagtcgac
     1201 gtacggcatg atcacctccg gccgctggcc gatgccctgg tcgtccgtgg cgatgaacgc
     1261 gatcgggaac acgtcagcgc caaggaaggt ccgggtccag gccagctccg aggagatgac
     1321 ccgcatcacg cgctggatgg tcggcagccg gaatgactgg gtatggggca ggttcgtctc
     1381 cgccagctcg tacggaccgt cgctgaagaa gcggatgtag tccagctgga cctcgtcgaa
     1441 gcccttcgcc gccaggtcgc cggcgatggc cgcgatgtac tcggccacgc ccgggttgcc
     1501 gggatcgagc catgtgccgc cgccccagtc ggtccacggc tggccggtgg acgtgttgcg
     1561 gacggcgagc tcgggacgag actggcccag ggtgttgtcc ttcatgacca ccacccgggc
     1621 gatggtgtag atcccgcgct ccttgagtgc ggggaggagc tcttccaggt cgaacaccgg
     1681 cgtgctcatc agcgcgcccg cgctgaccgc ctcgggcaga tcggtcgagt agtacagcca
     1741 gccaccggtc tccttgacgt cgatgaccat cgcgttgacc tcggtccggt caatgagatc
     1801 gagcatggca tccagcctcc cggccgcttc gaacaccgcc gacggcgcgt acagcgcccg
     1861 cgcgacgaac ggcgggagcg tgacgtcctt ggtcatctcc tcgtcgaccg gaatgacgcc
     1921 gagctggtat ccgggcgcct tatagatgag cgtcccttca gtgggcacgc cgtccagcgc
     1981 gtaggtcccg tcggtgccgg tctggacccc
//
LOCUS       DASYGU010000071         6554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1142536, whole genome shotgun sequence.
ACCESSION   DASYGU010000071 DASYGU010000000
VERSION     DASYGU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142536"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGO32_01795"
     CDS             complement(<1..738)
                     /locus_tag="VGO32_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7199516.1"
                     /translation="MTAGDPDANIRERRQLAEVAVAQAARGEWEAAAETNRQLLELGG
                     DVDAYNRLGKALSELDRHAEALEAYQAALERDKLNRIAERNIERLEALLTAEDAPRGD
                     RHATRAPAALFIEEMGKTGSARLINLAPAKQLAPLSAGDAIELKATDGELEARVRGIK
                     VGQVEPRVANRLLDLMANGNRYEAALTHVKDEDLRLIIRETFADPANFGRVSFPGAAA
                     GRSSDVRPYMKGSALRYDDDEESEESEE"
     gene            complement(873..1661)
                     /locus_tag="VGO32_01800"
     CDS             complement(873..1661)
                     /locus_tag="VGO32_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199517.1"
                     /translation="MSAVADGRRLFRTWRVVGSRAVKRYGPLVLPLLMAALVAAGWAV
                     DPVALAAVLALELALAGLGTAALLGAASSTTGYARYAIFAVAAVSVTLAGRLLPPGIG
                     PLAAPLAWGFLFWVLYIERHGPAMPVYRLSLDLTLVITVFSATAGMSGVIPPELWLLG
                     MVLFGALIAVPCLRAAEARGALGVPAVGHAALHLLVVLQVATALLLLDLPQPVGPALV
                     ALTFHSWSGAADALESGVSTRAVVLEYGSLAAIGLIVALFLSGA"
     gene            complement(1658..3409)
                     /gene="polX"
                     /locus_tag="VGO32_01805"
     CDS             complement(1658..3409)
                     /gene="polX"
                     /locus_tag="VGO32_01805"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEV7199518.1"
                     /translation="MPRPRSVRRSDAGGLSNAELAATFREIGDMLELMGEVPYKAVAY
                     RRAADAIERYPADLTPLVASGRMPKIAGVGAAIGARLTELATTGHLEYHERLREQIPP
                     GLVDVLRIGGVGPKTARQLHLELGIGSIEDLQAAAEAGRVRTVSGMSARTEQNILEGI
                     ARLEQQADQRLLLHEADRLAGELIEGLRKVASIVQIEAAGSLRRRRATIGDLDLLASS
                     DDPAALIAAFGRLPTVNRVIASGTDKASVTLAAEGDRRVDLMLCTPDAWGTHLVHFTG
                     SKEHNVALRERALDRGLSLSEKGFKVVDDGALLLAADEAEVYARLDLPWIAPELREGE
                     GEIAAAVDGRLPRLVERDDVRGDTHTHSDWTDGVDTIETMARAAQGRGLEYMVLTDHS
                     PSLGVTRGLSPERIIEQKHELDRLNGELAPFRILHGTEMEIRSDGSLDYPDELLAAFD
                     VVVASIHTGRSQPTEQLTRRALAAIENPHVDIIAHPTGRIVNRRDPVSYDWPQVFEAA
                     ARTGTRLEINGSPRLDLDERLARDAAKAGVQLTLASDAHRTEELVYLDYAVGVARRAW
                     LEPDQLANTSGIEGWNR"
     gene            complement(3498..3659)
                     /locus_tag="VGO32_01810"
     CDS             complement(3498..3659)
                     /locus_tag="VGO32_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199519.1"
                     /translation="MSRGLTYMALIASIRQRELLEEAHRERYTTEVPRRRRGRIFGMS
                     LPHVLRRTR"
     gene            3798..4451
                     /locus_tag="VGO32_01815"
     CDS             3798..4451
                     /locus_tag="VGO32_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEV7199520.1"
                     /translation="MTQIRDLMPEPPSGDLWVDFVNTLVYTHDEPADRVASAETLVGW
                     LRQQRLLSERAAGAERAALRDEPDEAQRRLERFRALRDLIRNMATEMATGGRPSRPNV
                     RELNHVLRDGLHYHQLEMDDDGSEYGLARVGDRLDQARATIAGTLAEFVATGPVDRLR
                     ICANAGCREIFIDRSRTGRRRWCDMRTCGNQAKAARHRARLRVIGTPEPLPAATVGH"
     gene            4679..6034
                     /gene="mgtE"
                     /locus_tag="VGO32_01820"
     CDS             4679..6034
                     /gene="mgtE"
                     /locus_tag="VGO32_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV7199521.1"
                     /translation="MSSVETRELLAEVQALIREREEEGVRGLAERIGPPEWADLVPQL
                     EPREVAVLMQWLPDEEVVGLLEELPPVEAARIIRTLAQPEAAELLEEMDPDDAADVIE
                     ELPDTTADEILIRMSPDDAAEIRRLAAYEPDTAGGLMTPDFVAVPADATAEQAIAAIR
                     QLADDAETANYVYVLDPDRRLLGVLSSYRLLLSPPETPVTELMAPDTVRVQVGADQEA
                     AARLLTDRNLLAIPVVDDADRLLGIITEDDVADVIQAEATEDIERLGGSQPLNLPYRL
                     ASVPLLVRKRVFWLLLLFVAAGYTGTVLQNFQSELTEVVALSFFIPLLIGTGGNIGSQ
                     TVTLIVRSMALNEVAMKDIWWIVFKEMRVAFVLGAVMAVAAFVRAYLLDVSPDIGIVV
                     ALTVVAICIWAATVAAALPLVLRRLRVDPAVVSAPLITTIVDGTGLIIYFEIARLVLG
                     L"
     gene            complement(6042..>6554)
                     /locus_tag="VGO32_01825"
     CDS             complement(6042..>6554)
                     /locus_tag="VGO32_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7199522.1"
                     /translation="KFTQILGNVNPVAGPYFNFSIPEPTGVVGVIAAEDSSLLGLVSR
                     LAPVIVSGNAAVLLSSESRPLPAITLAEVIATSDVPGGVVNVITGLRRELVPHLAAHM
                     DVNALDGFGLSPADVPAVEEAATDNVKRFSRPPTGGLDRYDWLSATAQSPYVIGEFVE
                     IKTVWHPIGV"
BASE COUNT          979 a         2275 c         2321 g          979 t
ORIGIN      
        1 ctcctcggac tcctccgact cttcgtcgtc atcgtaccgg agggcactgc ccttcatgta
       61 cggtcggacg tcactgctcc ggccggcggc cgctcccggg aacgaaaccc gcccgaagtt
      121 agccggatcg gcgaacgtct cgcggatgat cagccgtaga tcctcgtcct tgacatgcgt
      181 cagcgccgct tcataccgat tgccattggc catcaggtcc agcagccggt tggccacccg
      241 cggttccacc tgcccgacct tgatgccgcg cacgcgagct tccagctcgc cgtccgtggc
      301 cttgagctcg atcgcgtcac cggcggacag cggggcgagc tgcttggccg gcgcgaggtt
      361 gatcaggcga gcgctgccgg tcttgcccat ctcctcgatg aacagcgcgg ccggggcgcg
      421 ggtcgcatgc cggtcgccgc gcggggcgtc ctcggcggtg agcagggcct cgagccgctc
      481 gatgttccgc tccgcaatgc ggttcagctt gtcgcgttcg agcgcggcct ggtaggcctc
      541 cagcgcctcg gcatggcgat ccagctcgga cagggccttg cccagccggt tgtaggcatc
      601 cacgtcgccg ccgagctcga gcagctggcg gttcgtctcg gccgcggcct cccattcgcc
      661 gcgggcagcc tgagccacgg ccacctcggc cagctggcgg cgctcgcgga tattggcgtc
      721 aggatccccg gcggtcatgg gcgcgcgtct ctccccacgg cgaagtcgat cggcgataag
      781 tcatgggcgg acggtgccgc gtgcagcagg cggaaccgtc ggcgggcgac agtttgtaac
      841 accgagcccg cggagtgtca acgaattcgg gatcaggccc cgctgaggaa cagggccacg
      901 atcaggccaa tggccgccag ggagccgtat tcgagcacga ccgcgcgggt cgagacccct
      961 gattcgagcg cgtccgcggc gccgctccac gagtggaagg tgagggccac cagggccggg
     1021 ccgaccggct gcgggaggtc caggagcaga agcgcggtcg ccacctggag gacgacgagc
     1081 aggtggaggg cggcgtggcc gacggcgggc acgccgagcg cgccgcgtgc ctcggccgcg
     1141 cgcagacagg gcacggcgat cagggccccg aacaggacca tcccgagcaa ccacagctcg
     1201 ggagggatga ccccgctcat gcccgccgtg gcggagaaga cggtgatgac cagggtcagg
     1261 tccagcgaga gccggtagac cggcatggcc gggccgtggc gctcgatgta caggacccag
     1321 aacaggaatc cccaggccag cggagcggcg agcggaccga tgccgggcgg cagcagccgc
     1381 ccggcgagcg tgactgacac cgccgcgacc gcgaagatcg cgtaccgggc gtagccggtg
     1441 gtcgagctgg ccgcgcccag cagggcggcg gtgccgaggc cggccagcgc cagctcgagg
     1501 gcgagcacgg ccgccagggc gaccgggtcg acggcccacc cagcggcgac cagggcggcc
     1561 atcaggagcg gcaggaccag cggcccatac cgcttcacgg cgcgcgagcc gaccacccgc
     1621 caggtgcgga acaggcgccg gccgtcggcg acggcgctca tcggttccaa ccctcgatgc
     1681 ccgacgtgtt ggcgagctgg tccggctcca gccaggcgcg acgggcaacc ccgaccgcgt
     1741 agtcgagata gacgagctct tcggtccgat gcgcgtcgct ggccagggtg agctggacgc
     1801 cggccttcgc cgcgtctcgg gccagccgct cgtcgagatc gagccgcggg ctgccgttga
     1861 tctcgagccg ggtcccggtc cgagcggccg cctcgaagac ctggggccag tcgtaggaca
     1921 ccgggtcgcg gcggttcacg atccggccag tcgggtgggc aatgatgtca acgtgggggt
     1981 tctcgatggc cgccagcgcg cggcgggtca gctgctcggt gggctggctg cgaccggtat
     2041 ggatgctcgc caccaccaca tcgaacgcgg ccagcagctc gtcgggatag tcgagcgagc
     2101 cgtcggagcg aatctccatc tcggtcccgt gcaggatccg gaacggagcg agctcaccgt
     2161 tcaggcggtc cagctcgtgc ttctgctcga tgatccgctc cggggagagg ccgcgcgtga
     2221 cccccagcga cggggagtgg tcggtgagga ccatgtactc caggccacgg ccctgggcgg
     2281 cgcgggccat cgtctcgatc gtgtccaccc cgtcggtcca gtccgagtga gtgtgggtat
     2341 cgccgcggac gtcgtcgcgc tccaccagcc ggggcaggcg cccgtcgacc gcggccgcga
     2401 tctcgccctc cccctcgcgc agctcggggg cgatccacgg caggtcgagc cgggcgtaga
     2461 cctccgcctc gtcggcggcg aggagcagcg ctccgtcatc gacgaccttg aacccctttt
     2521 cgctgagcga cagcccacgg tccagggcgc gctcgcggag ggcgacgttg tgctccttgc
     2581 tgccggtgaa gtggacgagg tgggtgcccc acgcgtcggg cgtgcacagc atgagatcga
     2641 cccgtcggtc gccctcggcg gccagggtca cgctggcctt gtccgtgccc gacgcgatga
     2701 cccggttgac ggtggggagc cgcccgaacg cggcgatcag ggcagcggga tcgtccgagg
     2761 aggcgaggag gtccaggtca ccgatcgtgg cccggcgtcg ccgcagcgag ccggcggcct
     2821 cgatctgcac aatactggcc accttgcgca ggccctcgat cagctccccc gccaaccgat
     2881 cggcctcgtg aaggagcagc cgctggtcgg cctgctgctc gaggcgggca atgccctcga
     2941 ggatgttctg ctcggtccga gccgacatcc cgctcacggt tcggacacgg ccggcctcgg
     3001 cggccgcctg gaggtcctcg atggagccga tccccagctc gaggtgaagc tggcgcgcgg
     3061 tcttgggccc cacgccgcca atgcgcagga cgtcgaccag gcccggcggg atctgctcgc
     3121 ggagccgttc gtgatactcg agatggccgg tggtcgcgag ctcggtcagc cgcgccccga
     3181 tggccgctcc gacgccggcg atcttcggca tgcggccgga ggcgacgagc ggggtgaggt
     3241 cggcggggta gcgctcgatg gcgtcggccg ctcgccggta ggcgaccgcc ttgtacggca
     3301 cctcgcccat gagctcgagc atgtcaccga tctcgcgaaa ggtggccgcc agctcggcgt
     3361 tggagagccc gccggcgtct gaccggcgga cggaacgggg gcgaggcatg gcggccattc
     3421 taaggagcgc gccgattcgt tcccggaaac agcaacgccg ggcccgtcgg cccggcgttg
     3481 aatggttggt ggttgtctta tcgcgtccgg cgcagcacgt gcggcaggct catcccaaag
     3541 atgcgacccc ggcgacgccg cgggacttcg gtcgtgtacc gctcccggtg cgcctcctca
     3601 agcagctcgc gctgacgaat ggatgcgatc aaggccatgt aggtcagacc gcgactcatt
     3661 cgatacctcc tgtttcctct gcagttggga cgccggcgtt cccggtgtac cagcagtaac
     3721 tgttcacaat gcccttatcg gttacgattc taggggtaga caaataactt gtcaactggc
     3781 ctataatggt tactccgatg acccagatcc gagacctcat gcccgagcct ccctcgggcg
     3841 acctgtgggt cgacttcgtg aacaccctcg tctacaccca tgacgagccc gccgatcggg
     3901 tggccagcgc cgaaacgctg gtgggctggc tgcgccagca gaggctgctg tccgagcggg
     3961 ccgccggcgc cgagcgggcc gcgctccgcg acgagccgga cgaggcgcag cgtcgcctcg
     4021 agcgattccg ggccctgcgc gatctcatcc ggaacatggc caccgagatg gccaccggcg
     4081 gacgtccgag ccgcccaaac gtgcgcgagc tgaaccacgt cctccgtgac ggcctccact
     4141 atcaccagct cgagatggac gacgatgggt cggagtatgg gctcgcccgg gtcggagatc
     4201 ggctggatca ggcgagggcc accatcgccg ggaccctggc ggaattcgtc gcgaccggcc
     4261 cggtcgaccg cctccggatc tgcgcgaacg ccggttgccg cgagatcttc atcgaccgat
     4321 cacgaaccgg gcggcgccgg tggtgcgaca tgcggacctg tggcaaccag gccaaggccg
     4381 cgcgacaccg tgcgcgactg cgggtaatcg gtactcccga gccccttccc gcggcgacgg
     4441 tcgggcacta gaatcggggc agttccccgt atttcgagga cccatcgacc cagtggacgc
     4501 tggttgtagt acccgacatt ccgcgtgccg gcggccggcc ggtgccgagg tgagcgccta
     4561 gcgttcctcc tcgcctcccc ggcgaccgac ggccacccgg cgcgcgctcc acacgacgtc
     4621 tgcgcgacga ggtggtcgtt ccatgtccgc ctcgcccgcg ccgagaggag gtgatccgat
     4681 gagctcagtc gagacccgcg agctgctggc cgaggtgcag gcgctcatcc gcgagcgcga
     4741 ggaagagggc gtgcgcggcc tcgccgagcg catcggtccg ccggagtggg ctgacctcgt
     4801 cccccagctc gagccccggg aagtggccgt cctcatgcag tggctgcccg acgaggaggt
     4861 ggtcggcctc ctcgaggagc tgcccccggt cgaggcggca cggatcattc gcaccctggc
     4921 gcagccggag gcggccgagc tgctcgaaga gatggatccc gacgacgcgg cggacgttat
     4981 cgaggagctt cccgatacga cggccgacga gatcctgatc cggatgagcc ccgacgacgc
     5041 ggctgagatc cgccgactgg cggcctacga gccggacacc gccggcgggc tcatgacccc
     5101 tgacttcgtg gccgtccccg cggacgcgac ggccgagcag gcgatcgccg ccattcgcca
     5161 gctggcggac gacgcggaga ccgccaacta cgtctacgtc ctcgatcccg atcggcggct
     5221 gctgggcgtc ctgtcgtcgt accggctcct gctcagccca ccggagacgc ccgtgaccga
     5281 gctgatggcg ccggacaccg tgcgcgtcca ggtcggcgcc gaccaagagg ccgcggcccg
     5341 actgctcacg gaccgaaacc tcctggcgat cccggtggtg gacgacgcgg accgcctgct
     5401 cgggatcatc accgaggacg acgtggccga tgtcatccag gccgaggcaa ccgaggacat
     5461 cgagcgcctc ggtggttcgc agccgttgaa cctgccctat cgcctcgcga gcgtgccgct
     5521 cctggtccgg aagcgcgtct tctggctgct cctcctgttc gtggccgccg gctacaccgg
     5581 gaccgtgctc cagaacttcc agtccgagct gaccgaggtc gtcgccctct cgttcttcat
     5641 cccgctcctg atcgggaccg gcggcaacat cggttcgcag acggtcaccc tgatcgtgcg
     5701 ctcgatggcc ctcaacgaag tggccatgaa ggacatctgg tggatcgtgt tcaaggagat
     5761 gcgggtcgcc ttcgtccttg gcgcggtgat ggccgtggcc gcgttcgtgc gggcctacct
     5821 cctcgacgtg tcgcccgaca tcgggatcgt ggtcgcgctg acggtggtgg ccatctgcat
     5881 ctgggcggcc accgtggccg ccgccctgcc gctcgtcctc cgccggctgc gcgtggaccc
     5941 cgcggtcgtg tccgcgccgc tgatcaccac gatcgtggat ggcaccgggc tgatcatcta
     6001 tttcgagatc gcccggctgg tgctgggcct ctgaccgtcg gctagacgcc gatggggtgc
     6061 cagacggtct tgatctcgac gaactcgccg atgacgtacg gcgactgggc tgtggccgac
     6121 agccagtcat agcggtccag gccgccggtc ggtggccgcg agaagcgctt cacgttgtcg
     6181 gtggccgctt cctcgaccgc cggcacgtcc gcgggtgaca ggccgaagcc gtccagcgcg
     6241 ttgacgtcca tgtgcgcggc gaggtgcggg acgagctccc gtcggaggcc ggtgatgacg
     6301 ttcacgaccc cgcccggcac gtcggacgtg gcaatgacct cggcgagagt gatggccggc
     6361 agtgggcggg attcgctgga cagcaagacc gccgcgttcc ccgacacgat gaccggcgcc
     6421 aggcggctca cgagcccgag cagcgaggag tcctcggcgg cgatgacccc caccacaccc
     6481 gtgggctcgg ggatcgagaa gttgaagtag gggccggcca ccgggttcac gttgcccagg
     6541 atctgggtga actt
//
LOCUS       DASYGU010000072         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1091553, whole genome shotgun sequence.
ACCESSION   DASYGU010000072 DASYGU010000000
VERSION     DASYGU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091553"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VGO32_01830"
     CDS             complement(<1..1025)
                     /locus_tag="VGO32_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEV7199523.1"
                     /translation="MAIVATETRRRFSLPDNELPTHYYNIAADLPEPLPPPLHPGTKE
                     PIGPEMLAPLFPMELIRQEVSAERFIEIPEPVRDVYRIYRPSPLIRALGLERALDTPA
                     RIYYKYEGVSPAGSHKPNTAVAQAYYNQAEGVRRLTTETGAGQWGSALAFAGSLFGIE
                     TKVYMVKVSYEQKPFRRSFIETFGAQVVASPSTETNAGRQILAADPDSTGSLGIAISE
                     AIEEAASREDTKYSIGSALNHVLLHQTVIGQEAIKQMEMAGEEPDVIIGCAGGGSNFA
                     GLSFPWLGRTFRGGKTYRVIAAEPEAAPSLTRGTYAYDFGDTAQMTPLIKMHTLGHDF
                     VPEPIHAG"
     gene            1530..1931
                     /locus_tag="VGO32_01835"
     CDS             1530..1931
                     /locus_tag="VGO32_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199524.1"
                     /translation="MTREEHRTVVTDDPMRYPTRQVASEIHDIVESQPSGGEVARRVI
                     VLVFGIVQILLVLRIVLLLVAANQAQPLVNAILTLSQLFVAPFEGILGTDALSSNASI
                     LDVAAVVALIGWSVLELVILWAVNIFRRESA"
     gene            2106..2477
                     /locus_tag="VGO32_01840"
     CDS             2106..2477
                     /locus_tag="VGO32_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEV7199525.1"
                     /translation="MCYDGECPLCKESVARIKGVYHGRRIRWIPYQELQRDHPQLAEA
                     IGSRDLSSALHVIEADGTTVSSGAAAVLRIADVVPRLRIFARLGRLPVVKLLVEPMYG
                     FVARHRHRLSRLLGSEGSSAD"
     gene            complement(2519..>3970)
                     /locus_tag="VGO32_01845"
     CDS             complement(2519..>3970)
                     /locus_tag="VGO32_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199526.1"
                     /translation="LGFQLTVPQIVAAMLTCAVIEVALTFRQTRSFVWPASAMLTGSG
                     VALIMRVVGTPAHDPWNTFGWYIFAAVAGVSLLTKYVIKYRGSHVFNPSNIGLVLAFV
                     ILGSTRVEPLDFWWGPLDFWMLLAYAVIIVGGLLITRRLHLLALAGTYWVTLAVGLWI
                     LAASGHCMTAQWAFAPVCGVDYWRVIATSPEVLIFLFFMITDPKTIPASNVGRVVFGV
                     LVAVTGVLLMAPQTDEFGTKVGLLASLVIVCAARPLLDRVMPEPRSTADDLRAFAVRA
                     AGGPGAGFPRRVGGAAAAGVAIVVLGVGIVAAGTPARGTVVPDASEILDRLPSAVDPS
                     TFPPITVEQDVQDWNHEIAGPGAQELLLTLAQNLELENQALLAADAEILSAVDHGDRL
                     AEMRGRLDEATSSGTTMVAHYQFDEVNVTLLEPFGVQTGLSLGLEATGTVTTETYVDG
                     KLQGTDTAPYQHTFVMRQATGARWLNVDVLPGT"
BASE COUNT          622 a         1321 c         1376 g          651 t
ORIGIN      
        1 ccggcatgga tcggttcggg cacgaagtcg tggccgagcg tgtgcatctt gatcagcggc
       61 gtcatctggg cggtatcgcc gaagtcgtag gcataggtgc cgcgggtcag gctcggggcc
      121 gcctcgggct cggcggcgat gacgcgatac gtcttcccgc cccggaaggt ccggcccagc
      181 cacgggaacg acagcccggc gaagttggag ccaccgccag cgcagccgat gatcacgtcc
      241 ggctcctcgc cggccatctc catctgcttg atcgcctcct ggccgatgac ggtctgatgg
      301 aggaggacgt ggttcagcgc gctgccgatc gagtacttcg tgtcctcgcg gctggccgct
      361 tcctcgatgg cctcgctgat cgcgatcccc aggctgccgg tcgagtccgg gtcggcggcc
      421 aggatctggc gaccggcgtt cgtctcggtc gacggcgagg ccacgacctg ggccccaaag
      481 gtctcgatga agctccgccg gaacggcttc tgctcgtagg agaccttgac catgtagacc
      541 ttggtctcga tcccgaacag cgaaccggcg aaggccaggg cgctccccca ctggccggca
      601 ccggtctcgg tcgtcaggcg ccggacccct tcggcctggt tgtaataggc ctgggccacc
      661 gccgtgttcg gcttgtgcga gccggccggc gacacgccct cgtacttgta gtagatgcgg
      721 gccggggtat cgagcgcgcg ctcgaggccc agtgcccgga tcagcgggga ggggcgatag
      781 atgcggtaca cgtcccggac cggctccggg atctcgatga accgctcggc gctgacctcc
      841 tgcctgatga gctccatcgg gaacaggggc gccagcatct ccggaccgat gggctctttc
      901 gtgcccgggt ggagtggagg cgggagcggc tcgggcaggt cggccgcgat gttgtagtag
      961 tgggtcggca gctcgttgtc agggagcgag aaccgacgcc gggtctccgt ggctacgatg
     1021 gccattggtg gaacctccag agaacaacgc gacccctccg tcccaaagga cgagggggtc
     1081 tctcgtggtg ccacctttgt ttcccggtgt tctcgaccgg gctcattcga ccacggctgc
     1141 ggttgcccgc cgcgatggcc tcggctttgg tatcggtgcc tctccgccgg tggctactgt
     1201 ccgctggacc gaagtcgtcg ttccgcgagg ttcgcgcccg gagctcacgg gtccattcct
     1261 cggtcgcgcc gccgccgggg tctcactgct cccggctccc tggaggctcg cctgaccggg
     1321 tactcgtccc gatcaacgcc atattcaact gcggcgcgaa cgctacgccc ggtgtgcaga
     1381 gggtgtcaat cggtgaggcg cacctggtgc gagctcgagg caactgatgc ggttttgtca
     1441 tgcccacgac cgtgcccgca accgcccgcc cggtccccct cgggcacgat gaccagtctg
     1501 ttgcagccct cgggctgagg aggaccatcg tgaccagaga agagcatcgc accgtcgtca
     1561 cggacgaccc gatgcgctac cccacccggc aggtcgcctc ggagatccac gacatcgtcg
     1621 agtcccagcc gagtggcggc gaagtcgccc gacgggtgat cgtgctcgtc ttcgggatcg
     1681 tccagatcct gctcgtgctg aggatcgtcc tgctgctggt ggccgccaac caggcgcagc
     1741 cgctggtgaa cgcgatcctg accctcagcc agctgttcgt cgctccgttc gaggggatcc
     1801 tgggcaccga cgcgttgagc tccaatgcct cgatcctaga cgtggccgcg gtggtcgcgc
     1861 tgatcggctg gagtgtcctc gaactggtca tcctgtgggc ggtgaacatc ttccggcgcg
     1921 agtctgcctg aggtggacgt gatcgcaggc gccgtcatcg tggccgcgat tgccgtccca
     1981 gcgctcatcc tggcgctcat ggagatccgc catcttcgtc agatcgcgtg gctcagagcg
     2041 cgaatcggaa tcgtctagcc gaggtgggga cgaagggtcg cttgccagcg accggccaag
     2101 tgacggtgtg ctacgacggg gaatgcccgc tgtgcaagga atcggtggcc cgcatcaaag
     2161 gcgtgtacca cgggcgcagg attcgatgga tcccctacca ggaacttcag cgtgaccatc
     2221 cccagctcgc tgaggccatc gggtcgcgag acctcagctc ggcgctccac gtcatcgagg
     2281 ccgacggaac gaccgtgagc tcgggagcgg cggccgtcct ccgcatcgcg gacgtcgtgc
     2341 cccggctgag gatcttcgcg cgcctgggcc ggctcccggt cgtgaagctg ctggtcgaac
     2401 cgatgtacgg attcgtcgct cgtcatcgcc atcggctcag ccgactgctg ggctcggaag
     2461 ggtcgtccgc agactgacct gggcgactga cctggaagca gatccgcggc ctgagccgtc
     2521 acgtgcccgg caggacgtcc acgttgagcc agcgagcgcc ggttgcctgg cgcatgacga
     2581 acgtgtgttg gtacggagcc gtgtcggttc cctgcagctt cccgtccacg taggtctcgg
     2641 tcgtcaccgt cccggtcgct tcgaggccca ggctgagacc ggtctggaca ccgaacggct
     2701 cgaggagggt gacgttcacc tcgtcgaact gatagtgggc gaccatcgtc gtcccgcttg
     2761 acgtcgcttc gtcgaggcgg ccccgcatct cggccagccg gtccccgtgg tcgaccgcac
     2821 tcaggatttc cgcgtccgcg gcgaggagcg cctggttctc gagctcgagg ttttgggcga
     2881 gggtcaggag gagctcctgg gcgccgggac cggcgatctc gtgattccag tcctggacgt
     2941 cctgctcgac ggtgatgggt ggaaaggtcg aagggtccac ggcgctcggc agccggtcca
     3001 ggatctcgga cgcgtcgggc accaccgtgc cgcgggcagg ggtgcccgcc gccacgatgc
     3061 cgaccccgag caccacgatc gccactcctg ctgcggctgc gcctcccacc cgtcgcggga
     3121 agccggcccc cggaccgccc gccgcccgga ccgcgaaggc gcgaaggtca tcggccgtcg
     3181 agcgcggctc gggcatgacc cggtccagga gcgggcgggc cgcgcagacg atcaccaggc
     3241 tggcgaggag accgaccttg gtgccgaact catcggtctg cggcgccatg agcaggaccc
     3301 cggtcacggc caccagcacc ccgaacacga cccgtcccac gttgctggcc gggatcgtct
     3361 tggggtcggt gatcatgaag aacaggaaga tgaggacctc gggcgaggtg gcgatcacgc
     3421 gccagtagtc gacgccgcac accggcgcga acgcccactg ggcggtcatg cagtgaccgg
     3481 aggcggcgag gatccagagc ccgacggcca gggtcaccca gtacgtgccg gccagcgcca
     3541 ggaggtggag gcgccgggtg atgagaaggc cgccgacgat gatcaccgca taggccagca
     3601 gcatccagaa atcgagcggc ccccaccaga aatcgagcgg ctcgacccgg gtgctgccca
     3661 ggatcacgaa ggccaggacc aggccgatgt tcgacgggtt gaagacgtgc gaaccgcggt
     3721 acttgatgac gtacttggtc agcagcgaga cgcccgccac cgcggcaaag atgtaccagc
     3781 cgaaggtgtt ccacgggtcg tgggcaggcg tgccgacgac tcgcatgatc agggccacgc
     3841 cactgcccgt gagcatcgcg ctggcgggcc acacgaacga gcgcgtctgc cggaaggtga
     3901 gggcgacctc gatcaccgcg caggtcagca tcgcggccac gatctggggc acggtcagct
     3961 ggaagcccag
//
LOCUS       DASYGU010000073         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_577237, whole genome shotgun sequence.
ACCESSION   DASYGU010000073 DASYGU010000000
VERSION     DASYGU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577237"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="ftsA"
                     /locus_tag="VGO32_01850"
     CDS             complement(<1..635)
                     /gene="ftsA"
                     /locus_tag="VGO32_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV7199527.1"
                     /translation="MDRETVLVGIDPGSTKVTTLIGEVTPAGDVNVVGYGIAPSIGIK
                     KGMVANIEQTVQSIATSIEKAERLSGYKIGSAFVAVGGSHISSQNSRGVVAVSGHRRE
                     VSKEDIARATEAARAVQVPSNREILHVIPRGYIVDGQEGIKDPLGMSAVRLEVETHIV
                     AGASTSLQNLSKCVNSAGVQIDELVIASLAAAEATLTDTEKELGVIVADIGG"
     gene            complement(664..1005)
                     /locus_tag="VGO32_01855"
     CDS             complement(664..1005)
                     /locus_tag="VGO32_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEV7199528.1"
                     /translation="MSSNLRMWLPLVGLGLGILLGLLLNVSVSPELARYTAVAILAGL
                     DSILGAVRAELDGQYDNRVFLSGFVANTAVAVVLTFVGDRLGIDLYLVALIAFGLRIF
                     QNVALIRRHFL"
     gene            complement(1052..2002)
                     /locus_tag="VGO32_01860"
     CDS             complement(1052..2002)
                     /locus_tag="VGO32_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199529.1"
                     /translation="MSDRPLWERTRPLGRSRKTGAASTDPVRRPTTTRVRGRGRPARG
                     AAPRESTPSPRRRRRRINWTRITAAVLCLAFIGGASWLAAGPWLQIRAVTYAGADWTG
                     RAALDAVTDPIRGQSLLMVDAQATADELATLPGVQTADVDIGLFGDVHVSLVEGDAVV
                     RWRTDAAQLLVAEDGTVVGVLSLTATASPALGDLPVVEDQREGSHDLSVGDQLAAHEV
                     DAALLVSGLTPAQLGSQAAVLTVAIDQTFGFVVSAPQAGWQAAFGFYGVDPDDTPDVM
                     QARLQSQASGVRTLLAGHPEAGLAWIDARNPGRVYFRARG"
     gene            complement(1999..>2990)
                     /locus_tag="VGO32_01865"
     CDS             complement(1999..>2990)
                     /locus_tag="VGO32_01865"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEV7199530.1"
                     /translation="IYIDAAGGWHLPTGPAPPLGPDDSLARQLGAETITEHSARLEAG
                     DAALPVPLGGAALQAQAAVRTLAEAIDVAFLAVHGPFGEDGTLQGFLELAGIPYTGAG
                     VLASAVAMDKVVFKDLMRAHGLPVVAYTSYGRRAWRTAPERVIADALARIGPKSVVKP
                     ARLGSSVGMSLAHDPDELPSAIETAFGWDSKVIVEAYLPAAREFECGVLGNDQPVVFE
                     PGEVVSHHELYDYEAKYVPGLADVVVRADIDPALADRLRELARAAYAAVDARGMARID
                     FLAADDEVYISEMNTIPGFTATSMFPKQAEMAGIEFGDLVARLIELAEEEPPR"
BASE COUNT          454 a         1055 c         1048 g          433 t
ORIGIN      
        1 ccgccgatgt cggccacgat cacgcccagc tccttctcgg tatcggtcag ggtcgcctcg
       61 gccgcggcga gcgaggcgat caccagctcg tcgatctgga ccccggccga gttcacgcac
      121 ttgctcaggt tctggagcga ggtgctggcc ccggccacga tatgcgtctc gacttcgagc
      181 cggaccgcgc tcatgccgag cggatccttg atcccctcct gcccgtccac gatgtagccg
      241 cgcggaatga cgtgcaggat ctcgcgattg gacgggacct gtacagcgcg tgccgcctcg
      301 gtggcgcggg cgatgtcctc cttggagact tcacggcgat ggccgctgac cgccaccacg
      361 cctcggctgt tctgggacga gatgtggctg ccgccgacgg ccacgaacgc ggatccgatc
      421 ttgtagccgg acagacgctc ggccttctcg atgctggtcg cgatcgactg caccgtctgc
      481 tcgatgttgg cgaccatccc cttcttgatc ccgatcgagg gagcgatccc gtagcccacc
      541 acgttcacgt cgccggcggg cgtgacctcg ccgatgaggg tggtgacctt cgtgctgccg
      601 gggtcgatcc cgacgaggac cgtttctctg tccaaactca gcctccctga agcgcggccg
      661 accttacagg aagtggcggc ggatcaatgc cacgttctgg aagatgcgaa gaccgaaggc
      721 gatgagggcg acgagataca ggtcgatgcc gagccgatcg ccgacgaagg tgagcacgac
      781 cgcgaccgcg gtgttggcca cgaagccgct gaggaacacg cggttgtcgt actggccgtc
      841 gagctcggcc cggacggcgc cgaggatcga gtcgagcccg gccaggatgg cgacggccgt
      901 gtagcgggcc agctcggggc tcacgctcac gttcaggagc aggcccagca gaatgccgag
      961 ccccagccca acgagaggaa gccacatccg gaggttcgag gacaccgcgc ctggtcggga
     1021 tccgggcgcc gccgccgaac ggcgagcggt ctcagccccg ggcacggaaa tataccctcc
     1081 ccggattgcg ggcatcgatc caggccagtc cggcctcggg atggccggcc aacagggtcc
     1141 gcactccact tgcctgggat tgaagccgag cctgcatgac gtcgggcgtg tcgtcggggt
     1201 cgacgccgta gaacccgaac gcggcctgcc agccggcctg cggggccgag accacgaacc
     1261 cgaaggtctg gtcgatggcc acggtcagga cggccgcctg cgatcccagc tgggccggag
     1321 tcaggcccga gaccaggagc gcggcgtcca cctcgtgcgc ggcgagctgg tccccgacgg
     1381 agaggtcgtg cgagccctcc cgctgatcct cgaccaccgg caggtcgccc agggccgggc
     1441 ttgcggtcgc ggtcagggac aggacgccga ccaccgtccc gtcctcggcc accagcagct
     1501 gggccgcatc ggtccgccag cggacgacgg cgtcgccctc caccagcgac acatggacgt
     1561 ctccgaacag gccgatgtcc acgtccgcgg tctgcacccc gggcagcgtc gcgagctcat
     1621 cggccgtggc ctgcgcgtcg accatcagga ggctctgccc tcggatcggg tcggtcaccg
     1681 cgtccagggc cgcacgcccg gtccagtcgg cgccggcgta ggtcaccgcc cgaatctgaa
     1741 gccaggggcc ggccgccagc cagctggccc cgccgatgaa cgcgaggcac agcaccgccg
     1801 ccgtgatccg ggtccagttg atccgccgac gccgacggcg cggagacggt gtcgactcgc
     1861 gcggcgcggc gccacgcgcc ggccggccac ggccgcgaac ccgcgtggtg gtcgggcgtc
     1921 gcaccggatc ggtggacgcc gcaccggtct tccgagaccg gcccagcggg cgggtgcgct
     1981 cccacagcgg ccggtcgctc atcggggcgg ctcctcctcg gccagctcga tgagccgggc
     2041 gacgaggtct ccgaactcga tgccggccat ttccgcctgc ttggggaaca tcgaggtggc
     2101 cgtgaagccc gggatcgtgt tcatctcgga gatgtagacc tcgtcgtcgg cggcgaggaa
     2161 gtcgatccgg gccatgcccc gggcatcgac ggcggcgtat gcggctcgag ccagctcgcg
     2221 gagccgatcg gcgagggcgg ggtcgatgtc ggcgcggacc acgacatcgg ccaagccggg
     2281 aacgtacttc gcctcgtagt cgtacagctc gtgatggctg acgacctctc ctggctcgaa
     2341 gacgaccggc tggtcgttgc ccagcacgcc gcactcgaac tcgcgcgccg cgggcaggta
     2401 cgcctccacg atcaccttcg agtcccagcc gaacgcggtc tcgatcgccg atggcagctc
     2461 gtcgggatcg tgggcgaggc tcatccccac gctgctgccg agacgtgcgg gtttgaccac
     2521 cgatttcggc ccgatgcggg ccagcgcatc ggcgatcact cgttcgggtg ccgtccgcca
     2581 ggccctgcgg ccgtacgacg tgtaggcgac gaccgggagg ccgtgggcgc gcatgaggtc
     2641 cttgaacacg accttgtcca ttgccaccgc actggccagc acgccggccc cggtgtacgg
     2701 gatcccggcc agctcgagaa agccctggag cgtgccgtcc tcgccgaatg ggccgtgaac
     2761 ggccaggaac gcgacgtcga ttgcctccgc cagggtgcgg accgcggcct gggcttgaag
     2821 ggccgcaccg ccgagcggca cgggcagcgc ggcgtcccca gcttccagcc gggcgctgtg
     2881 ctcggtgatc gtttccgccc cgagctggcg ggccaggctg tcgtccgggc caaggggcgg
     2941 agccggtccc gtcggcaggt gccagccacc ggcagcgtcg atgtagatga
//
LOCUS       DASYGU010000074         5058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_165422, whole genome shotgun sequence.
ACCESSION   DASYGU010000074 DASYGU010000000
VERSION     DASYGU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165422"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VGO32_01870"
     CDS             <1..1016
                     /locus_tag="VGO32_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEV7199531.1"
                     /translation="AAKGFGAEGIGLARTEHMLLGDRRELVERVVTNTGRDEALARIE
                     EVTRHDFLEILDEMDGLPVVVRLLDPPLHEFLPDLVELSTRAAVSDDRGQHDPGLEHL
                     LAVVRRWHEVNPMLGLRGVRLLAVVPELLDAQVRALGEAVAELLSLGRHPRLEIMVPL
                     VCDVEELRRARTRIEVTLTDVSRRKGLDLAFPIGVMIELPRAALTSGDLAQSAEFFSF
                     GTNDLTQTTWGISRDDAETSFLGLYRQQGLLSFDPFDTLDERGVGRLMELSCSEGRAA
                     RPGLGLGLCGEHGGDPASVRFCADLGLDYVSCSPPRVPVARLEAGRAAVLAEMSGAGS
                     DTR"
     gene            2062..3780
                     /locus_tag="VGO32_01875"
     CDS             2062..3780
                     /locus_tag="VGO32_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEV7199532.1"
                     /translation="MTLPDSPGARQRGWLRALPAMRGLLPIDRSHVPADLLAGLTLAA
                     LGIPEVLGYAKIAGMPVVTGLYTMLLPMAVFAVLGSSRHLVVAADSATAVILAAALTG
                     LAVAGSPRYVQLAGLAALLAGAMLLLTRLARLGFLANFLSRTLLVGFLTGVGIQVAAG
                     QLPDMLGVTAAGEQTPARLLDTLRALPHLSRADVAVSAGVILVVLAARKVTRRIPGPL
                     IPGALIAVILAIAVSRAADLAGRGVAVVGPVPGGLPSFGLPALGWQDAAALLGPAASM
                     FVVILAQSAATSRAYAVKYEEAFSEDTDLVGLGAANTVAAFSGTFVVNGSPTKVQMVD
                     SAGGRSQLSQFTAAAVVLLVLLVLTGPLAYLPAAALAAVVFLIAIGLIDVAGMRLILA
                     ARRHEFAVALLTTAAVVGLGVEYGIVIAVIASIIEHLRHSYNPRNGVLAKSPAGHWQT
                     APVAPGARTEEGLVVYRFGTSLYYANAFRLLDDVTGLVGHGGPLRWIVLDCAAIGDID
                     YTASAVLTTVIEHAHQRHIRFVVTTVLGPVREQLDRYGISAALGPNAYYDTPGEALEA
                     FHAAEG"
     gene            complement(4324..4998)
                     /locus_tag="VGO32_01880"
     CDS             complement(4324..4998)
                     /locus_tag="VGO32_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7199533.1"
                     /translation="MWFHTFALGHGIYTPGLARDHVYRLSVLGADRFAGRSVLDVGAF
                     DGFYSFLAERRGARRVVAVDNEQYVDWVQARFGISLTGGAGFHAIAGVLDSNVDYRRM
                     DALDVRSFGERFDLVLCFGILHRVTDPIALLRALADVLTPAGELIIETYGSHLSGDIP
                     AVEVHEPGEVYQRDDFVYWGFPAEGLRRLARIAGLTDVEVMAAPDIDGHPRLLAILRS
                     GEPSGP"
BASE COUNT          774 a         1807 c         1689 g          788 t
ORIGIN      
        1 aggcggccaa gggtttcggc gccgagggca tcggcctggc ccgtaccgag cacatgctgc
       61 tgggcgaccg gcgcgagctg gtcgaacggg tcgtgaccaa cactggccgt gatgaggccc
      121 tggccagaat cgaggaggtg acccgacacg acttcctcga gatcctcgac gagatggacg
      181 ggctcccggt cgtggtccgg ctgctcgacc caccgctaca cgagttccta cccgacctcg
      241 tcgagctgtc gaccagggcg gcggtcagcg acgaccgcgg ccagcacgac cccggcctgg
      301 aacacctgct cgcggtggtc cggcgctggc acgaggtcaa ccccatgctc ggtctgcggg
      361 gggtgcggct gctcgccgtc gtccccgagc tgctcgacgc ccaggtccgc gccctcgggg
      421 aggcggtcgc cgaactgcta tcgctagggc gccacccgcg cctggagatc atggtgccgc
      481 tggtgtgcga tgtggaggag ctgcgccggg cccgcacccg aatcgaggtg acgctgaccg
      541 acgtgtcccg ccgcaaaggc ctggacctgg ccttccccat cggcgtgatg atcgagctgc
      601 ccagggcggc cctgacctcg ggcgacctcg cccagagcgc cgagttcttc tcgttcggca
      661 ccaacgacct gacacagacc acgtggggga tctcccggga cgacgccgag accagcttcc
      721 tcggcctcta ccggcagcag ggcctgctgt cattcgaccc cttcgacacc ctcgacgaac
      781 gcggggtcgg ccggctgatg gaactctcgt gctctgaagg acgtgcggcc cgcccaggcc
      841 tcggcctggg cctatgcggt gagcacggcg gcgacccagc ctcggtgcgg ttctgcgccg
      901 acctcggact cgactacgtc tcctgctcac ctccgcgggt gccggtcgcc cggctggaag
      961 ccggccgggc agccgtcctc gccgagatgt cgggcgccgg ctcggacacc cgctgaacgg
     1021 accttcccgc cgagggtgaa actccatctc tcggacccct ccggggctta caaccagcgc
     1081 gtggattctc tttgctgcgc acccacccac ggtcggccgg ctagccggaa cttcagcaac
     1141 tcagcgacga agatgcggat tctagggctc catgccacgg ccatgactcc ccgtcgtcac
     1201 cgccggcgac cacggggaag ccgaccagtc cgtccgtgct tgtcgttgac ccccagcgtg
     1261 cgaatctccg ccgcacgccg gttctcgtcg gcgtcctcga gccgggtcat gagccagacg
     1321 gggatgcgcg agcggacgag gtggacccat cgactatcgc gcagaactgc ggcccgactg
     1381 tggcagcgaa cgcgtggccg ccgtggtggg ccgcctccgc gtcgacggcg cggacccctg
     1441 atcgccgcgg gcatcaacca gggccacaat ggcctgcgcc cgagacattt ccctcctgcc
     1501 gaatgaggta gggcatcgtc gcgtccccgt cgtggcccac ccgcagcatc tggctcacct
     1561 caccgtggtc tcgatccaca ggcgctgatg ggcgggtccg ttcgcagagc cgatcgttgt
     1621 cgatggcgat tccggcgggc cgccgccaac gcaacgacaa tcgcctggcc atcagcgttg
     1681 agctcgaaag ggcgtatttg aggggcctcc tcgaatccga tggattaggt gggcactacc
     1741 aacatggcgc cgggaggtaa cccacgaagg agcggaggcc ccgctcctac ctcgcatcag
     1801 ggcgcggccg gacaccatcg atcgaggctc tgaaaccgct acccgcggtt tcggccatcc
     1861 cgcgtattcg gggatgcgcg gcgaggccgc tcgcggtcgt cgttgcgaag gagtccccgt
     1921 agctcccccg cggtgtccgc ctccagggtc cgagcgtcct ggaggcgggc ggcgagaatc
     1981 tggaggctgc tgtgaccgca ggaaacgcgt ggcggcggga tgatgaacga acccgaaacc
     2041 gcaggggaac cggggggtgc tgtgacgttg ccggattcgc ctggggcccg ccagcgaggc
     2101 tggctgcggg cgctgcccgc gatgcggggg ctgctgccga tcgaccggtc ccacgtaccc
     2161 gcggatctgc tcgcgggctt gacgctggcc gccttgggca tccccgaggt cctcgggtac
     2221 gcgaagatcg ccgggatgcc ggtggtgacc ggcctgtaca cgatgctgct gccgatggcc
     2281 gtgttcgccg tcctcggatc gtcgcgccac ctggtggtgg ccgcggattc cgcgaccgcg
     2341 gtcatactcg ccgccgcgct taccggtctc gcggtggctg ggtcgccacg ctacgtccag
     2401 ctggccgggc tcgcggccct gctcgccggg gcgatgctgc tgcttacccg gctggccaga
     2461 ctggggttcc tggcgaattt cctctcccgc accctgctgg tcgggttcct taccggcgtc
     2521 ggcatccagg tggctgccgg ccagcttccc gacatgctcg gggtgaccgc ggcgggcgaa
     2581 cagacaccgg ccaggctcct ggacacgctg cgcgcgctcc cgcatctcag tcgggcagac
     2641 gtggcagtgt cggccggggt catcctggtc gtgcttgcgg cgcgcaaggt cacccgcagg
     2701 atcccggggc cgctgatccc gggggcgctg atcgcggtga tcctcgcgat cgcggtaagc
     2761 cgggccgcgg atctggccgg ccgcggcgtg gccgtagtcg ggccggtccc aggcggcctt
     2821 ccgagcttcg ggctgccggc cctgggctgg caggacgccg ccgcgctcct cggccccgcg
     2881 gcgtcgatgt tcgtggtcat cctggcccaa agcgcggcca cctcccgtgc ctacgccgtc
     2941 aagtacgagg aggcgttcag cgaagacacc gacctggtcg gcttgggcgc cgccaatacc
     3001 gtcgccgcat tctccggcac cttcgtcgtc aacggcagcc cgaccaaggt gcagatggtc
     3061 gacagcgcgg gcgggcgcag ccagctgtcc cagttcaccg ccgccgcggt ggttctcctt
     3121 gtcctactgg tgctcaccgg gccgctcgcc tacctgccgg ctgccgcgct agcggcggtg
     3181 gtcttcctca tcgctatcgg gctcatcgat gtcgccggga tgcggctcat cctggccgcc
     3241 cggcgccacg agttcgccgt ggccttgctc acgacggccg cggtggtggg gctgggggtc
     3301 gagtacggca tcgtgatcgc cgtcatcgcc tcgatcatcg aacacctgcg ccacagctac
     3361 aacccacgca acggcgtgct ggcgaagtca cccgccgggc actggcagac cgccccggta
     3421 gccccaggtg cccggaccga ggaagggctg gtggtctacc ggttcggcac cagcctctac
     3481 tacgccaacg ccttcagact cctcgacgac gtcaccggcc tggtcggcca cggcggcccg
     3541 ctgcgctgga tagtgctcga ctgcgcggcc atcggagaca tcgactacac cgcatccgca
     3601 gtcctcacca cggtcatcga acacgctcac caacggcaca tccgcttcgt cgtcaccacc
     3661 gtcctcggcc cggtgcgcga gcagctcgac cgctacggca tcagcgccgc cctcggcccc
     3721 aacgcctact acgacacccc cggcgaggca ctcgaggcct tccacgccgc agaagggtaa
     3781 gtggtgcgta ctaggcggtg agtaacggtg cacgcaccgg gactccgcgg gacgtgtgag
     3841 ccgaaggagc agggcacggc cgtcctggaa tggcggagcc ccgagattcg agaatcgtcg
     3901 gggcttcgtc tgtcaggatg gaacgacgtc ctgacctccg gttcaggttt catcaatccg
     3961 ctcgcctcgt ccaaggcgct cactgcggtg tcgacccgca gccgcggatt ccgattcctt
     4021 cggaccgcca gcgttgccgt tggcgtacct caagcatctc tcgaccgagt cgaccgcaat
     4081 agtgacctaa gtcccgaccc ttccttccga cggacctgat gcaaagaccc atcggccccc
     4141 ggggtggaca aggtacccgg ggagccgatc ggtcgatcct ccgtcggccg acggctacaa
     4201 cccagaccca ccgtggcctt ctgcgggacc tgcgggctta gcgtctgtga ctgcgggtag
     4261 ccgtgaggag cctcgcccaa cgtgaacccc atcccagggc aaccgaagaa agcaacgccc
     4321 ctactacgga cccgagggtt ctccggatcg gaggatggct aggagccggg gatggccgtc
     4381 gatgtctggc gcggccataa cttcgacatc ggtcagcccg gctatgcgag caagccgacg
     4441 cagtccctcg gcggggaaac cccaatagac gaagtcgtcg cgctgataca cctcgccggg
     4501 ctcgtggacc tcgacagccg ggatgtcgcc cgacaggtga gaaccgtagg tctcgatgat
     4561 cagctccccg gccggcgtca ggacgtccgc caacgcccgc agcaacgcga tcgggtcggt
     4621 cacccgatgc aggatcccga agcacaggac caggtcgaag cgttcgccaa agcttcggac
     4681 gtcgagcgcg tccatccgcc ggtagtcgac gttcgagtcg aggacgccgg caatcgcgtg
     4741 gaagccggca ccgccggtca gagagatgcc gaaccgggcc tggacccagt cgacgtactg
     4801 ctcgttgtcg accgcgacaa cgcgacgcgc gccccgccgc tcggccagga agctgtagaa
     4861 gccgtcgaac gccccaacgt caagcacact gcgcccggcg aagcggtccg cgcccagcac
     4921 cgaaagacgg tagacgtgat cccgggccag gcccggcgtg tagatcccgt gccccagcgc
     4981 gaaggtgtga aaccacaacg gaaccggccg gtgtcgctcc ttcgaccgtc tcccgggcgt
     5041 tctgtcgtcc cggccggg
//
LOCUS       DASYGU010000075         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1348781, whole genome shotgun sequence.
ACCESSION   DASYGU010000075 DASYGU010000000
VERSION     DASYGU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348781"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGO32_01885"
     CDS             <1..814
                     /locus_tag="VGO32_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV7199534.1"
                     /translation="LAAAEAAGLPAPAAVGVASPGPLDPQRGVVHQPPNLPGWDEVPL
                     AHRLSDAFGVPAFLERDTKVAMLAEWRHGAAVGARHAIYVTVSTGIGGALVVDGHLVD
                     GPDSIAGEVGHLTVDLDGPPDGEGAPGHVEGIASGAALARDGAALLERGEAPGLAALV
                     SSGTPVDAAAVCAAADRGDGACRLLLDRAWSAIGATCAGLVNLLNPEVIVLGGAIADH
                     RPDLHAAVRAEIAVRAFPIPAARAQVRASRFGENVSLVGCWPLIHDTEVQGP"
     gene            811..1821
                     /gene="gap"
                     /locus_tag="VGO32_01890"
     CDS             811..1821
                     /gene="gap"
                     /locus_tag="VGO32_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV7199535.1"
                     /translation="MTTRVAINGFGRIGRQTLRAILERHPDELEVVALNDLAPTDTNA
                     HLFRYDSTYGRYQGTVEAGEGEITVDGHAIRAFSEKDPAALPWTDLGVQIVVESTGVF
                     TDGEKAKAHLQAGATKVIISAPARNEDLTVVLGVNENRYDSARHHIVSNASCTTNGLA
                     LPAKVVFDTFGIDHGLMTTVHSYTNDQPVLDVFHKDLRRARSAGQNIIPTTTGAAKAL
                     ALVIPELRGRFDGFSLRVPTATVSVIDFVAQTARPVTAEAANAAFREAAAGPMHGLLG
                     YTEEPLVSMDFKGDDHSSIVDGLSTMVTGDRLLKVIAWYDNEWGYSCRVADLAAFMGA
                     RL"
     gene            1818..3035
                     /locus_tag="VGO32_01895"
     CDS             1818..3035
                     /locus_tag="VGO32_01895"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV7199536.1"
                     /translation="MTVGKFKPLHKQTVRDVSVGGKRVFLRSDLNVPLDDGRITDDTR
                     IRASLPTLVYLLEQGAIVIMASHLGRPHGKVNDAYRLKPVADRLSQLLGRPVRMTGDA
                     LGPGVQDAINKLRPGDLILLENLRFHAGEEANDPAYAEALAGFADLYVDDAFGSAHRA
                     HASTEGITHFLPAVAGILLEREVNALSRLLNKPPRPFHTVIGGAKISDKLDVLRVLVG
                     RCQAILVGGGMANTFLAARGLEMGKSLVETEQLENASAIMAEARRRRMRLILPTDVLV
                     AAQVHHRAPREVVSVNAVPKDWMVVDIGPHTVAKYRQALQAAKTIFWNGPMGIFEVAP
                     FAEGTNEIARAIAERTAKGVITVVGGGDSVAAVQQLGLSEQMSHVSTGGGASLEFVEG
                     RTLPGVTALLDRA"
     gene            3032..>3619
                     /gene="eno"
                     /locus_tag="VGO32_01900"
     CDS             3032..>3619
                     /gene="eno"
                     /locus_tag="VGO32_01900"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919866.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV7199537.1"
                     /translation="MTDRAIRRVHAREILDSRGNPTVEVEVTLEGGVTGRAAVPSGAS
                     TGTHEAVELRDGDASRFGGKGVLRAVANVRGEIAGAVTGRDATDQAGLDQALIDLDGT
                     PNKARLGANAVLGVSLATAHAAAVATGQPLYRYLGGERAATLPVPMVNILNGGRHAAG
                     GTDFQEFMVIPLGAATFSQGIRWTAEIFHALGALLH"
BASE COUNT          544 a         1295 c         1235 g          545 t
ORIGIN      
        1 cctggcggct gccgaggccg ccggtctgcc cgctcccgcg gcggtcgggg tggcgtcgcc
       61 cggccccctc gatccgcagc gtggcgtcgt ccaccagccc ccgaaccttc ccggctggga
      121 cgaggtcccg ctcgctcacc gcctgtcgga cgcgttcggg gtgcccgcgt tcctcgaacg
      181 ggacacgaag gtcgcgatgc tcgccgagtg gcgccacggc gccgcggttg gtgcgcggca
      241 cgcgatctac gtcaccgtct cgaccggcat cggcggggcg ctggtcgtgg acggccacct
      301 ggtcgatggt ccggattcga ttgccggcga ggtcggccat ctgaccgtgg acctcgacgg
      361 gccacccgac ggcgagggcg ccccgggaca cgtggagggg attgcctccg gcgcggcgct
      421 ggcccgtgat ggcgcggccc tcctcgagcg cggtgaggca cccggattgg cggcgctcgt
      481 ctcgtcgggc acgcccgtgg acgcggcggc ggtgtgcgcg gcggccgata ggggagacgg
      541 cgcgtgccgc ttgctcctgg accgcgcatg gagcgcgatc ggggcgacct gcgccgggct
      601 cgtcaacctg ctcaaccccg aggtaatcgt gctgggcggg gcgattgccg accaccgtcc
      661 cgacctccac gctgcggttc gggccgagat cgccgttcga gcgtttccca ttccagcagc
      721 ccgggcgcag gtccgggcct cccgattcgg cgagaacgtt tccctcgtcg gctgctggcc
      781 gctcatccac gatacggagg ttcagggccc atgaccaccc gggttgcgat caacggattc
      841 ggccgcatcg gccgccagac cctccgcgcc atcctcgagc gccatcccga cgagctcgag
      901 gttgtggctc tcaacgatct ggcgccgacc gatacgaacg ctcatctgtt ccgttacgac
      961 tcgacctacg gccgctacca gggcacggtg gaagccggcg agggcgagat caccgtcgac
     1021 ggccacgcga tccgcgcctt ctccgaaaaa gatccggccg ccctgccgtg gacggatctc
     1081 ggggtccaga tcgtggtcga gtcgaccggc gtgttcaccg atggcgagaa ggcgaaagcc
     1141 catctccagg cgggagcgac caaggtcatc atcagcgctc cggcccggaa cgaggacctc
     1201 accgtggtcc tgggcgtaaa cgagaaccgg tacgactccg cgcgtcacca catcgtcagc
     1261 aacgcctcgt gcacgaccaa cggtctcgcc ctgccggcca aggtcgtgtt cgacacgttc
     1321 ggcatcgacc acgggctgat gaccaccgtc cactcgtaca ccaacgacca gccggtgctg
     1381 gacgtgtttc ataaggacct gcgccgcgcg cggtccgccg gtcagaacat catccccacc
     1441 accaccggcg ccgcgaaggc gctggccctc gtcatccctg agctgagggg ccgcttcgac
     1501 ggcttttcgc tgcgcgtgcc caccgccacg gtcagcgtca tcgacttcgt ggcccagacc
     1561 gcccggccgg tgaccgccga ggcggccaac gccgccttcc gcgaggcggc cgccggaccc
     1621 atgcacggcc tgctgggcta caccgaggag ccgcttgtca gcatggactt caagggtgac
     1681 gaccactcgt cgatcgtgga cgggctctcg accatggtca ccggcgatcg gctgctcaag
     1741 gtcatcgcct ggtacgacaa cgagtggggc tacagctgcc gggttgccga tctcgcggcg
     1801 ttcatgggcg cccgcctgtg acggtcggga agttcaagcc gctccacaag cagaccgtcc
     1861 gcgacgtctc cgtcggcggc aagcgcgtct tcctccgctc cgatctgaac gtcccgctgg
     1921 acgacgggcg gatcaccgac gacacccgga ttcgggccag cctgcccacc ctcgtatacc
     1981 tgctcgagca gggcgcgatc gtgatcatgg ccagccacct tggtcggccg cacggaaagg
     2041 tgaacgacgc gtaccggctc aagccggtgg cggatcggct cagccagctg ctcggtcgcc
     2101 cggtgcgcat gacaggcgat gccctcggcc ccggggtgca ggacgcgata aacaagctcc
     2161 gccccggtga cctgatcctc ctcgagaacc tgcgcttcca cgccggcgag gaggcgaacg
     2221 atccggccta tgccgaggcg ctggccgggt tcgccgacct gtacgtggac gacgcgttcg
     2281 gctccgccca tcgggcccac gcctcgaccg agggcatcac ccatttcctg cccgctgtcg
     2341 ccggcatcct cctcgagcgc gaggtcaatg ccctgtcgcg gctgctcaat aagccgcccc
     2401 gcccattcca cacggtcatc ggcggggcga agatctccga caagctcgac gtcctccgcg
     2461 tcctcgtcgg ccgctgccag gcgatcctgg tcggcggcgg gatggcgaac acgttcctcg
     2521 ccgccagggg gctcgagatg ggcaagagcc tggtcgagac cgaacagctc gaaaacgcgt
     2581 ccgcgatcat ggccgaggcc cgccggcggc ggatgcggct cattctgccc actgacgtgc
     2641 tcgtcgccgc ccaggtccac catcgggcgc ctcgcgaggt cgtatccgtc aacgcggtgc
     2701 caaaggactg gatggtggtc gacatcggtc cccacaccgt ggccaagtat cgccaggcgc
     2761 tccaggcggc taagaccatc ttctggaatg gaccgatggg aatcttcgag gttgccccgt
     2821 tcgccgaggg cacgaacgag attgcccggg ccattgccga gcgaacggcc aagggcgtga
     2881 tcacggtggt gggcggtggc gactcggtcg ccgcggttca gcagctcggg ctcagcgagc
     2941 agatgagcca cgtgagcacc ggcggcgggg cgtcgctcga gttcgtcgag ggacggaccc
     3001 tgcccggcgt caccgcgctc ttggaccggg catgaccgat cgcgctatcc ggcgcgttca
     3061 cgcccgcgaa atcctcgact cgcggggcaa cccgaccgtc gaggtggagg tcacccttga
     3121 aggtggcgtc acggggcgag cggccgttcc gagcggggca agcaccggga cccacgaggc
     3181 cgtcgagctc cgcgacggcg atgcctcgcg attcgggggc aagggcgtgc tgcgggccgt
     3241 ggcgaatgtc cggggcgaga tcgcgggcgc cgtcaccgga cgcgatgcca ccgaccaggc
     3301 gggcctcgac caggcgctga tcgatctcga cgggacgccg aacaaggccc ggctcggcgc
     3361 gaatgcggtg ctcggagtct cgctggccac cgcccacgcc gccgcggtcg ccaccggcca
     3421 gccgttgtat cgctatctcg gcggggagcg ggcggcgacc ctgccggtgc cgatggtgaa
     3481 catcctcaac ggtggacgtc atgccgccgg tgggaccgac ttccaggagt tcatggtcat
     3541 cccgctgggg gcggccacct tcagccaggg catccgctgg acggccgaga tcttccacgc
     3601 gctcggcgca ctgcttcac
//
LOCUS       DASYGU010000076         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_628706, whole genome shotgun sequence.
ACCESSION   DASYGU010000076 DASYGU010000000
VERSION     DASYGU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628706"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGO32_01905"
     CDS             <1..193
                     /locus_tag="VGO32_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199538.1"
                     /translation="LEADRQAETNIRAHSPATNGGTTQQIWVVDRNPFPLVIVGGFGS
                     DEQAGRDTIEEILASIDLS"
     gene            219..295
                     /locus_tag="VGO32_01910"
     tRNA            219..295
                     /locus_tag="VGO32_01910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:253..255,aa:Arg,seq:tct)
     gene            355..1023
                     /locus_tag="VGO32_01915"
     CDS             355..1023
                     /locus_tag="VGO32_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV7199539.1"
                     /translation="MSEKRTTARAFHDADGVVDWRVLFSGAHAYFPVGSFAEAARFVA
                     AIADVAEVVGHFPDVDVRPEGVTVRTASGEYGALSHRDVELARQISAEARKLRIGPDP
                     SQVQVVGIAVAQDAGSDVGPFWAAVLGYRSLGPKGDVVDRHRRNPYVSFQSLRPPKPG
                     RGRTHIDVSVPADQAEARVAAALAAGGRLADGTHVPDWWTLASPENHGVDIAAWPDVE
                     DGED"
     gene            complement(1025..>1996)
                     /locus_tag="VGO32_01920"
     CDS             complement(1025..>1996)
                     /locus_tag="VGO32_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV7199540.1"
                     /translation="SLGTLPADHLLVARAEALRAEMGLDSLLALDAADTFEPSVHLRP
                     MNASPLTFLASLLDAERAAADHRIVAVIGNSLGWYSALAVGGALDFDDGFRLVQGIAQ
                     LQEEAATEGLGGGQLIYPRVGADWQPVAEYTEALELALDGAETDGAEKPDRAFTSVDL
                     GAYVVLAASEAGIARLLDVLPPVRVGERQYPIRLAHHGPYHTPLLADVASAAQARFST
                     LDWRAPSATLIDGRGVRFTPWATNPVDLAWYTLGDQITTPYRFAASARVALREYAPDV
                     LVLAGPGNTLGGVTGQQVVAEGYRGIRTRADFESAQTEHPLVLSMRS"
BASE COUNT          326 a          679 c          692 g          299 t
ORIGIN      
        1 tttggaggcg gataggcagg cggagaccaa tatccgcgcc cactcgcctg caacgaacgg
       61 cggcacgacc caacagatct gggtcgtaga ccggaacccg ttcccgctcg tcatcgtcgg
      121 agggttcggt tccgacgagc aggccggacg cgacacgatc gaggagatct tggcgtcgat
      181 cgatctaagc tgaccgctcc ccggtaagat tttgggacgt ccccgtagct cagtggacta
      241 gagcggctgc cttctaagca gcgggtcgca ggttcgaatc ctgccgggga cgccaaaccc
      301 ctcccctgtc cgaatcgctt cccccacatc tccacgcctg cctagcattg ccccatgagc
      361 gaaaagcgaa cgacggctcg ggccttccac gacgccgatg gcgtcgtgga ctggcgggtc
      421 cttttctctg gcgcccacgc ctacttcccc gtcggatcgt tcgcggaagc cgcacggttt
      481 gtggccgcga tcgccgacgt tgcggaggtc gtgggccact tccccgacgt ggacgtgcga
      541 cccgagggag tcaccgtccg gaccgcctcc ggggagtacg gagctctgag ccatcgtgat
      601 gtcgagctgg cacgccagat ttcggcagaa gctcggaagc tgcggatcgg gccggacccc
      661 tcccaggtcc aggtcgtggg cattgcggtg gcacaggatg caggcagcga cgtaggtccg
      721 ttctgggccg cagtcctcgg gtacaggagc ctcggaccga aaggagacgt cgtcgatcgg
      781 caccgacgta acccgtacgt gtcgttccaa agcctccgcc ctcccaagcc ggggcgtggc
      841 cggacccaca tcgacgtcag cgtgccggcc gatcaggccg aagcacgagt cgcggcagca
      901 ctcgcagcgg gcggacgcct ggcggacggt acgcatgtgc ccgattggtg gacactcgcg
      961 tcgcccgaga accacggggt cgacatcgcg gcctggccgg acgtcgagga cggtgaggac
     1021 tgagtcagct ccgcatggac aggacgagcg gatgctcggt ctgcgctgat tcgaagtcgg
     1081 cccgcgttct gatgccccga tagccctcgg ccaccacctg ctggccggtc acgccgccca
     1141 gcgtgttccc cggcccggcc aggaccagga cgtcgggtgc gtattcgcgg agcgcgacac
     1201 gggcgctggc cgcgaagcgg tatggcgtgg tgatctggtc gccgagggtg taccaggcga
     1261 ggtcgaccgg gttggtggcc cacggggtga accggactcc acgaccatcg atcagggtcg
     1321 ccgatggcgc acgccagtca agggtcgaga atcgggcctg cgccgcgctc gcgacgtcgg
     1381 ccagcagggg cgtgtggtac ggcccgtgat gggccagccg gatcgggtac tggcgctcgc
     1441 ccacgcgcac cggcggcagc acgtcgagca gacgcgcgat gcccgcctcc gaggcggcca
     1501 gcaccacgta cgcgccgaga tccacgctgg tgaacgcccg gtccggcttc tcggctccat
     1561 cggtctcagc tccgtcgagc gccagctcga gcgcctcggt gtactcggcc accggctgcc
     1621 aatcggcgcc gacacgcggg tagatcagct ggccaccacc caagccctcg gtcgccgcct
     1681 cctcctggag ctgggcgatg ccctggacca gtcgaaaccc gtcgtcgaaa tccagtgcgc
     1741 cgcccacggc cagcgcgctg taccagccga gggagttgcc gatgacggcc acgatccggt
     1801 ggtcggccgc cgcccgctcg gcgtcgagca gcgaggcgag gaacgtgagc ggcgatgcat
     1861 tcatcggccg gagatggacc gaaggctcga atgtgtcggc cgcgtcgagg gcgagcaggc
     1921 tgtcgagtcc catctccgcg cgcagcgcct cggcccgggc gacgagcaga tggtcagctg
     1981 gaagcgtgcc gagcga
//
LOCUS       DASYGU010000077         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1400244, whole genome shotgun sequence.
ACCESSION   DASYGU010000077 DASYGU010000000
VERSION     DASYGU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400244"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VGO32_01925"
     CDS             complement(<1..972)
                     /locus_tag="VGO32_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199541.1"
                     /translation="MNDEPRSQPRRGRPRRRPDGGGGGGGPRRHGAPRGNFQRRRDLA
                     GGYSSVYAGGYPQALDEPALTLGELQEKSIEELRDLATEYGIEGDSLEHSDLVLKLLD
                     AVPLAQASTREEMVAGEVIGVLEIVDEGFAFLRREGVMPSPDDIYVSSSIVRRFGLRT
                     GDRVVGQSRPPKDQERYWGLARVDTVNGIDPELARRRPNFDALVPVFPDEMFDLEIDQ
                     KNLTQRLINLIAPVGKGQRGLILSPAKAGKTTVLKNIAHGITENHPDALLMVALIGER
                     PEEVTDMQRSVKGEIFASTFDEPTENHTRVAEMTLEIAKRQVETGRDV"
     gene            complement(1063..2658)
                     /locus_tag="VGO32_01930"
     CDS             complement(1063..2658)
                     /locus_tag="VGO32_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199542.1"
                     /translation="MTRTAGRRSATRRRAAGAFDLAAYEAEVEAFCGEQGEAQYANLA
                     GFRPDLDLAPVYGRHAGLFSTTAIDALREAASRADAEALRARRLLWFAVAGHLEQAVA
                     VTTDRIATAESTAVIVWRRERIGYRLAPIRIADISDRSERNALDERYRLAMEAINPLR
                     EERWTTLHARARELGAADYVALVADTSGLDPDGVAAGASQFLRDSETPYYAALRRYLA
                     LIEIEGGDGTVADLGHLLRGRGWDNYFDGSRLVGALATTMAGLGIDIANQPGTTIDLE
                     PRPAKSPRAFCVPIRVPGDVRLVVQPRGGHADFDGTLHELGHLEHFAHINAKLPPADR
                     YIGDETVTEGWAFLFQYLMSEPEWLARELRMPPDAIAGWLDFSAFRKLYFLRRYSAKL
                     LYELRLHRGGDWIVHRAEYAGLLGLLTGVRTPDTAFLADVDDGLYAARYLRAWLFEGS
                     VSAALRDQFGGTWWRSAGAGDWLRETWRAGLGPSAEDVVAALGYDHLDWRPVLRQIRA
                     QLIGEMSGYGGPNITTRAGSRKV"
     gene            complement(2666..>3494)
                     /locus_tag="VGO32_01935"
     CDS             complement(2666..>3494)
                     /locus_tag="VGO32_01935"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV7199543.1"
                     /translation="KYTELPDAYISVSEALKHAGLHHRVDVKVRWIGAEHFERVEPEE
                     VARELDGVAGVLVPGGFGYRGVEGMIRAIRWARENEIPYLGLCLGLQCAVIEFARSAL
                     GTDDANSSEFNVFTEHPVIDLMPDQADVTEKGGTMRLGLYPARLDEETRVRAAYGAEV
                     VYERHRHRFEVNNGYRERLGEAGMLWSGVSPDERLVEFIELRDHPWFVATQAHPELKS
                     RPNRPHPLFRDFVGAAASRLGLPTAPAEDSVGDQKPAPTRRRATRTRAASGAGRTEP"
BASE COUNT          517 a         1202 c         1235 g          540 t
ORIGIN      
        1 gacgtcccgg ccggtctcca cctggcgctt ggcgatctcg agggtcatct cggccacgcg
       61 ggtgtggttc tcggtcggct cgtcgaaggt ggaggcgaag atctcgccct tgaccgaccg
      121 ctgcatgtcg gtgacctcct ccggccgctc gccgatgagg gccaccatca gcagcgcgtc
      181 ggggtggttt tcggtgatgc cgtgggcgat gttcttcagg accgtggtct tgcccgcctt
      241 ggccggcgac aggatcaggc cacgctggcc cttgccaacc ggcgcgatca ggttgatgag
      301 ccgctgggtc aggttcttct ggtcgatctc gaggtcgaac atctcgtcgg ggaagaccgg
      361 gaccagcgcg tcgaagttcg gccggcggcg ggccagctcg ggatcgatcc cgttgaccgt
      421 gtcaacccgg gccaggcccc agtagcgctc ctggtccttc ggggggcggc tctggccgac
      481 gacccggtca ccggtgcgca gcccgaagcg acggacgatg cttgatgaca cgtaaatgtc
      541 gtccggtgaa ggcatgacgc cctcgcggcg gaggaaggcg aagccctcgt ccacgatctc
      601 cagcacgccg atcacctcgc cggcaaccat ctcctcgcgg gtggatgcct gggccagggg
      661 aaccgcgtcc aggagcttca ggacgaggtc cgagtgctcg aggctgtcgc cttcgatccc
      721 gtactcggtg gcgaggtcgc gcagctcctc gatagatttc tcctgcagct cgcccagggt
      781 cagggccggc tcgtcgagcg cctgcgggta gccaccggca tagaccgatg aatacccgcc
      841 cgcgaggtcg cggcggcgct ggaagttgcc gcgcggagcg ccgtgccggc gcggaccgcc
      901 gccaccacca ccaccatcag ggcggcggcg cggtcgtccg cgtcgcggct ggctgcgagg
      961 ttcgtcattc atgggggaga catctccatc agggaacagg ggagcgcatt gcgggccaca
     1021 tggccgcgtg gggctccagg aggggtgatc gtcggcgtcc ggtcagacct tgcggcttcc
     1081 ggcccgggtc gtaatgttcg gcccgccgta gccactcatc tcgccgatca gctgcgcgcg
     1141 gatctggcgc aggaccggcc gccaatcgag atgatcatac ccgagcgcgg caacgacgtc
     1201 ctcggcagat ggtcctagtc cggcccgcca cgtttccctc agccagtcgc ccgcaccagc
     1261 gctccgccac cacgtgccac cgaactgatc ccggagcgcc gcggacacgc tgccctcgaa
     1321 cagccaggcg cgcaggtagc gcgcggcata gagtccgtcg tcgacatcgg ccaggaaggc
     1381 ggtgtccggg gtccgcacgc cggtcagcag gccgagcaga cccgcgtact ccgcgcggtg
     1441 gacgatccag tccccacccc gatgcagccg cagctcgtac aggagcttgg ccgaatatcg
     1501 gcgcaggaaa tacagcttgc ggaaggcgct gaagtcgagc cagccggcga tcgcgtcggg
     1561 cggcatgcgc agctccctcg ccagccattc cggctcggac atcaggtact ggaacaggaa
     1621 tgcccagccc tcggtcacgg tctcgtcacc gatgtagcga tccgccggcg ggagcttggc
     1681 gttgatatgg gcgaaatgct cgagatggcc tagctcgtgg agcgtgccgt cgaagtccgc
     1741 gtgaccgccc cgcggctgga cgacgagccg gacgtcgccc ggaacccgga tcgggacgca
     1801 gaaggcgcgg ggcgacttgg ccggacgcgg ctcgaggtcg atcgtcgtcc cgggctggtt
     1861 cgcgatgtcg atcccgagcc cggccatggt cgtggccagg gccccgacta gccgcgagcc
     1921 atcgaagtag ttgtcccatc cccgaccgcg gaggagatgg ccgaggtcgg ccaccgttcc
     1981 gtcgccgccc tcgatctcga tgagggcgag gtatcggcgg agggcggcgt agtacggcgt
     2041 ctcggaatca cggaggaact gggacgcgcc ggcggccacg ccgtccggat cgagtcccga
     2101 agtgtccgcc acgagggcca cgtagtcggc ggctccgagc tcgcgcgctc tagcatggag
     2161 cgtggtccag cgctcctcac gcagtgggtt gatcgcctcc atcgccagcc ggtagcgctc
     2221 gtcgagcgcg ttccgctcgg agcgatcgct gatgtccgcg atccggatcg gcgccagccg
     2281 gtacccgatc cgctcgcggc gccagacgat caccgcggtg gactcggccg tggcgatgcg
     2341 gtcggtggtg accgcgacgg cctgctcgag atggccggcg accgcgaacc acaggagacg
     2401 cctggcacga agggcctccg cgtccgccct ggacgcagcc tcacggaggg catcgatcgc
     2461 ggtcgtcgag aagaggcccg cgtgacggcc gtagaccggc gcgaggtcga ggtcaggtcg
     2521 gaagccggcc aggttcgcgt actgcgcttc tccctgctcc ccgcagaacg cctccacctc
     2581 ggcctcgtag gccgccaggt cgaaggcgcc cgccgcacgg cgccgagtcg cgctgcggcg
     2641 cccggcagtc cgggtcatgt gtcggtcagg gttcagtccg gcccgcgccg gaggccgccc
     2701 gcgtcctggt cgctcggcgc ctggtcgggg ccggcttctg gtcaccgacc gaatcctcgg
     2761 ccggggcggt gggcaggccc agccggctgg ccgcggcgcc gacgaagtcg cggaacagcg
     2821 gatggggccg attgggcctg ctcttcagct cggggtgggc ctgggtcgcg acgaaccacg
     2881 gatggtcgcg gagctcgatg aattcgacca gccgctcgtc gggactgacc ccgctccaga
     2941 gcatgcccgc ctcacccagc cgctcccggt acccgttgtt gacctcgaac cgatgccggt
     3001 ggcgctcgta gacgacctcc gcgccatacg cggcacggac ccgtgtctct tcgtcgaggc
     3061 gggccgggta gaggccgagc cgcatggtgc cgcccttctc ggtgacatcg gcctgatcgg
     3121 gcatgagatc gatcaccggg tgctcggtga agacgttgaa ctcgctgctg ttcgcgtcgt
     3181 cggtgccgag ggctgatcga gcgaactcga tgaccgcgca ctggagaccg aggcacagcc
     3241 cgaggtacgg gatctcgttc tcgcgagccc agcgaatggc gcggatcatc ccctccaccc
     3301 cgcggtagcc gaagccaccg ggcacgagga cgccggccac gccgtcgagc tcgcgggcaa
     3361 cctcctcggg ctcgacccgt tcgaagtgct cggcgccgat ccagcggacc ttgacgtcca
     3421 cccggtggtg gaggcccgcg tgcttgagcg cctcgctgac gctgatgtag gcatctggca
     3481 gctcggtgta cttg
//
LOCUS       DASYGU010000078         5191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1812275, whole genome shotgun sequence.
ACCESSION   DASYGU010000078 DASYGU010000000
VERSION     DASYGU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812275"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VGO32_01940"
     CDS             complement(<1..407)
                     /locus_tag="VGO32_01940"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HEV7199544.1"
                     /translation="MASPVWVPTLERTDLPDPPALDGLRELAENLWWTWHPEAQRLFA
                     RIDPQGWARARNPIRLLRETDADRWATLAADERFVADTSTIVRRLHDDLENGADGLGG
                     DLGGPVAYLCAEFALHESLPVYSGGLGVLAGDLL"
     gene            478..2370
                     /locus_tag="VGO32_01945"
     CDS             478..2370
                     /locus_tag="VGO32_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199545.1"
                     /translation="MDSYSVNEAAAVLGIPEGRVWELLARGVLAGSTEVGGDMRVVLR
                     GATLPDAAKPAAPNAFRQDANGGNGENGGGHGELTPFRELLTEFRNLTERYGQALLAL
                     GESRGEVAALRGRVDLLEARIEHRLPWTEAPRAGWNAEALSEPESSDAEPADPIETEE
                     LSSVDALPPEEVVEAARDEPMAEDVVAEDPIAEEVVAEVVAEDATADEVVAEGVVDED
                     PIAEEVVAEEGAGMADAETEARAEQRETDEAASDEQHTEEVKPRVSARSRSREAVSGF
                     AEALARAEDPTTAEVLDGEESLPGGEDIADAIAAYRDEVAADAAPVVRVGYSTATPEP
                     DWIAEEDLLVIGAAGDRATDVLSPEAPEFQVVGAEDAPEEDALTGDVPAEETPPADVL
                     AREVDTETAAATIDEDEPAAWGAAPDASLEAETPASQVVESDEDQPPATSDSDYAWLD
                     DEPEAEPTPRIAALAIGEPDESSAVAQQVTPDQPQARTPIEIAAEPPASDDVALLVGP
                     VAFSEPPQIPREPAFWEPGARARAPFAVPHMDAWQASASEAMRVATEPEASRPEASRP
                     EAPWPEVEVGWGSTPAPTGASASEASPSETSPSASPAPVRPKPAVRRRRGPAARAIRR
                     LRILLD"
     gene            2385..2987
                     /locus_tag="VGO32_01950"
     CDS             2385..2987
                     /locus_tag="VGO32_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7199546.1"
                     /translation="MAAYDPRTALLVVDVQNDFADPDGGLSVPGGATIVPKLNREIRA
                     AIEAGASVIYTQDWHPSSTPHFAKDGGIWPVHCVGETWGAAFHPELLVAGPSVRKGVD
                     GGDGYSGFSVRDPASGDEHATELESLLLERGVERVVIGGLATDYCVRETTLDAIERGF
                     TATVLTDAVAAVDLQPGDGAAALDAMAAAGALLAASDDGG"
     gene            2993..3682
                     /locus_tag="VGO32_01955"
     CDS             2993..3682
                     /locus_tag="VGO32_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199547.1"
                     /translation="MDVLGVPARRLGNGMVAFGAVGLVVTVILAVAWLGGFVALQDLD
                     DRLEADREAVATALANTATLMDSTAAALEDVTASMGNMGAALTDASDLLDRLATTTDS
                     LATSLNVSILGQRPFAGAAENLETIASDLDTFAGHSAALAEDLETLEPSLGTLAGDLR
                     TVQESVATLATRVQDFGGIERLVGVVRLYVLLSAALAVWLAVLAGGCIWVGRQLQQAA
                     ITGSGEPTPTS"
     gene            complement(3648..3842)
                     /locus_tag="VGO32_01960"
     CDS             complement(3648..3842)
                     /locus_tag="VGO32_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199548.1"
                     /translation="MRRAIPVFVGVLIALTGAVWILQGLGVIVSSSLMTNDRTWVVLG
                     AVAVVAGAGLSWWGWARRSR"
     gene            3985..4356
                     /locus_tag="VGO32_01965"
     CDS             3985..4356
                     /locus_tag="VGO32_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV7199549.1"
                     /translation="MSISHSGGSRVTYSDQVLACVDCGTEFVFSASEQSFFAEKGFTS
                     PPKRCPSCRAQRRASGGYGGSSSSSSYGSGGGYGGGYDRGPREMHDAVCARCGKETQV
                     PFKPTGTRPVYCSDCFRLVRG"
     gene            4493..>5191
                     /locus_tag="VGO32_01970"
     CDS             4493..>5191
                     /locus_tag="VGO32_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7199550.1"
                     /translation="MSADQEQDLWSELGQQLRVDSIRSSSAAGSGHPTSSMSAADLMA
                     VLLAGHLRYDFDRPEDPRNDHLIFSKGHASPLLYAMYVAAGAIDEAEMLTFRQQGSRI
                     EGHPTPILPWVDVATGSLGQGLPIGVGVALAGKLLDHLPYRVWVLCGDSEMAEGSMFE
                     AMEHAAHAGLDNLTAIIDVNRLGQTGETMHGWDLDAYLRRAQAAGWHALAIDGHDVVQ
                     IDRAYADAAATELRP"
BASE COUNT          801 a         1770 c         1828 g          792 t
ORIGIN      
        1 agcaggtctc cggccagcac ccccagcccg cccgagtaga ccggcagcga ctcatggagg
       61 gcgaattcgg cgcagaggta ggcgaccggt ccgcccagat ctccgcccag gccgtccgca
      121 ccattctcga ggtcgtcgtg cagacggcgg acgatcgtgg acgtatccgc cacgaatcgt
      181 tcgtcggcgg cgagggttgc ccagcggtcg gcatcggtct cccggagcag ccgaatcggg
      241 ttgcgggcgc gggcccagcc ctggggatcg atccgggcaa agaggcgctg tgcctccggg
      301 tgccacgtcc accacaggtt ctcggccagc tcgcgcaggc cgtccagggc cggcggatcg
      361 ggcaggtccg tgcgttcgag ggtcggaacc cagacgggtg aggccatcgg cgccggatga
      421 tatcggtccg cgactcgtca tggcggttcg cgacgcggta cgctacgact ccccctgatg
      481 gacagctact cggtcaacga ggcggcggct gtcctcggca tccccgaggg acgtgtctgg
      541 gagcttctcg ctcgcggcgt ccttgctggg tctaccgagg ttggcggcga catgcgggtc
      601 gtactgcgcg gcgcaaccct gcccgacgca gcgaagccgg ctgctccgaa cgcgtttcgg
      661 caggacgcga atggcggaaa cggcgagaac ggcggtggcc atggcgagct cacgcccttc
      721 cgcgagctgc tcaccgagtt tcggaatctg accgagcgct acggccaggc gctgctcgct
      781 ctgggcgagt cgcggggcga ggtcgccgcc ctgcgcggcc gggtggatct gctcgaagcg
      841 cgcatcgagc atcggttgcc gtggaccgag gccccgcgtg cgggatggaa tgcggaggcc
      901 ctatccgagc cggagtcctc tgacgcggaa cccgcggatc cgatcgagac ggaagaactc
      961 agctctgtgg atgccctgcc ccccgaggaa gtcgtcgagg cggcacggga tgagccgatg
     1021 gccgaggacg tcgtcgccga ggacccgatt gccgaagagg tcgtcgccga ggtcgtcgcc
     1081 gaggacgcga ctgccgatga ggtcgttgcc gaaggcgtag ttgatgaaga tccgattgcc
     1141 gaagaggtcg ttgccgagga gggagccggt atggcggacg cggaaaccga ggctcgtgcg
     1201 gagcagcgcg agaccgatga ggccgcgagc gacgaacagc acaccgagga ggtcaagcct
     1261 cgagtgtcgg cccggagccg atcccgtgag gcggtctccg gattcgcgga ggcacttgcc
     1321 cgcgcggagg acccgaccac cgccgaggtt ctcgacgggg aggagtcgct ccccggcggt
     1381 gaggacatcg ccgacgcgat tgcggcctac cgcgatgagg tggccgccga cgcggccccg
     1441 gtggtccggg ttggctacag caccgcgact ccggaaccgg actggatcgc ggaggaagac
     1501 ctgctggtga tcggcgccgc gggcgatcgc gccacggacg tcctgtcgcc cgaagccccc
     1561 gagtttcagg tcgttggggc ggaggacgct ccggaagagg acgctctgac cggtgacgtc
     1621 ccggccgagg agaccccgcc tgccgacgtc ctggcccgtg aggtcgatac tgagaccgcg
     1681 gcggcaacga ttgacgagga tgagccggcg gcatggggcg cggctccgga cgcgtcgttg
     1741 gaggccgaga cacctgcatc gcaggtcgtc gagtccgacg aggatcagcc gcccgcgaca
     1801 tcggacagcg actatgcgtg gctggacgac gagcctgaag ctgagccaac gccacgaata
     1861 gcggccctcg ccatcggtga gcccgacgag tcgtcagccg tggcccagca ggtgacgccc
     1921 gaccaacccc aggcgcgcac gccaatcgag attgccgccg agccgccggc atctgatgac
     1981 gttgctctgc tggtcgggcc ggtcgcgttc tctgagcccc cgcagattcc gcgtgagccg
     2041 gcgttttggg agcctggagc tcgcgctcgg gcgccgttcg ccgtgccgca tatggacgcc
     2101 tggcaggcat cggcatcgga ggccatgcgg gtcgcgaccg agcccgaggc gtcccggccc
     2161 gaggcgtccc ggcccgaggc gccgtggccc gaggtcgagg tcggctgggg atcgacgcca
     2221 gcaccgaccg gcgcatccgc gagcgaagcg tcgccgtccg agacgtctcc gtccgcctcg
     2281 cctgcgccgg ttcgcccgaa acccgccgtc cgccgccggc gcgggccggc cgcccgcgcg
     2341 atccgccgcc tccgcatcct cctcgactga ccgccaccgc gcgcgtggct gcctacgacc
     2401 cgcgcaccgc gctcctggtg gtcgacgtgc agaacgactt tgccgatccc gatggcggcc
     2461 tgagcgttcc cggcggggcg acgatcgtcc cgaaactgaa ccgcgagatc cgcgcggcta
     2521 tcgaagcggg ggcgagcgtg atctacacac aggactggca cccgtccagc acgccgcatt
     2581 tcgcgaagga cggcgggatc tggccggtgc actgcgtcgg cgagacttgg ggtgccgcat
     2641 tccatccgga gctgctcgtc gcgggcccgt cggttcgcaa gggggttgat ggtggggacg
     2701 gctattcggg cttctcggtc cgagatccgg cctcagggga cgagcacgcg accgagctgg
     2761 aatccctcct cctcgagcgt ggcgtcgaac gggtcgtcat cggcggcctg gccaccgact
     2821 actgcgtgcg cgagaccacg ctggacgcga tcgagcgcgg attcacggcg acggtcctga
     2881 ccgatgccgt cgccgcggtc gatctccagc ccggtgatgg ggccgccgca cttgacgcga
     2941 tggccgcggc tggcgcgctt ctggccgcgt ccgacgacgg aggatgaccc cggtggacgt
     3001 gctgggcgtg cctgcccgac ggcttggcaa tgggatggtc gcgttcggcg ccgttggcct
     3061 ggtggtgacg gtcatcctgg ccgttgcctg gctcggcggg ttcgtcgcgc tccaggatct
     3121 ggacgaccgc cttgaggccg atcgcgaggc ggtcgcaacc gcgttggcca ataccgccac
     3181 gttgatggac agcaccgcgg cggccctcga ggacgtgaca gcgtcgatgg gcaacatggg
     3241 cgcggctctg accgacgcgt cggacctgct cgatcgactg gcgacgacca cggacagtct
     3301 ggccacctcg ctgaacgtca gtatcctcgg ccagcggccg ttcgccggcg ccgccgagaa
     3361 cctcgagacc attgcctccg atctggacac gttcgccggc cattcggccg ccctcgccga
     3421 ggacctcgag accctcgagc cgtctcttgg caccctggcc ggcgatctgc ggaccgttca
     3481 agaatcggtg gccaccctcg ccacccgggt acaggacttc ggcggtatcg agcggctggt
     3541 cggggttgtc cggctttacg ttctgctgtc cgccgccctc gcggtctggt tggcggtcct
     3601 cgccggcgga tgcatctggg tcggccgcca gcttcagcag gcggcgatca ccgggagcgg
     3661 cgagcccacc cccaccagct gaggccggcg ccggcgacga cggcgaccgc gccgagcacg
     3721 acccaggtcc ggtcgtttgt catgaggctg gacgacacga tcacgcccag cccctggaga
     3781 atccacaccg ccccggtgag agcgataagc acgccaacaa agacggggat cgccctgcgc
     3841 atgcgccgat ggtagacttt tacccggtcc ggaccgctcg tccggcgtcc aggcctttcc
     3901 cctcccctcc accacggttg tttcgctggc cacggggtag tggcaagacc cgtggcctgc
     3961 caccgagcga gcgacaacca ggccatgtcc atctctcaca gtggagggtc aagggtgacc
     4021 tattccgatc aggttctcgc gtgtgttgac tgcgggaccg agtttgtctt ttccgcctcg
     4081 gagcagagct tcttcgcgga gaaaggcttc acatcccccc cgaagcgatg cccatcgtgc
     4141 cgcgcccagc gccgcgcgtc gggtggctac ggtggcagca gcagcagcag cagctacggc
     4201 tcaggcggtg gttatggcgg tggctatgac cgcgggccac gcgagatgca tgacgccgtg
     4261 tgtgcacggt gcggcaagga gacccaggtc cccttcaagc caacgggcac ccgtccggtg
     4321 tactgcagcg actgcttccg actcgttcga ggctagttag accgatggcg ccggccgggc
     4381 cggcgcgcaa ttcggcgaaa gcggcgccca accgggcgcc gctttcatat ttctgcaaca
     4441 tcgatcatcc aggcttcgca ggcaaagcca tctcgatggg tatcattcga tcgtgagcgc
     4501 cgatcaggag caagacctct ggtccgagct gggccagcag ctccgcgtgg actccatccg
     4561 gtcgtcgagc gcggcgggat ccggacaccc cacctcgtcc atgtccgccg cggacctgat
     4621 ggcggtcctc ctcgctggtc acctgcgcta cgacttcgac cgtcccgaag acccgcgaaa
     4681 cgaccacctc atcttcagca agggccacgc cagcccattg ctgtacgcga tgtacgtcgc
     4741 ggccggcgcc atcgacgagg ccgagatgct cacgtttcgc cagcagggca gccggatcga
     4801 agggcatccc acgccgatcc tgccctgggt ggacgtcgcg accgggtcgc tcggccaggg
     4861 ccttccgatc ggtgtgggcg tggccctcgc cggcaagctg ctcgatcacc tgccctaccg
     4921 ggtatgggtg ctgtgcggtg acagcgagat ggccgagggt tcgatgttcg aggccatgga
     4981 gcatgcggcg cacgcgggcc tggacaacct gaccgcgatc atcgacgtca atcgcctcgg
     5041 ccagaccggc gagacgatgc acggctggga tctcgatgcc taccttcgtc gcgcgcaggc
     5101 ggcgggatgg catgcgctcg cgatcgatgg ccatgacgtg gtccagatcg accgcgctta
     5161 cgccgacgca gccgcgaccg agctgaggcc g
//
LOCUS       DASYGU010000079         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_886083, whole genome shotgun sequence.
ACCESSION   DASYGU010000079 DASYGU010000000
VERSION     DASYGU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886083"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..949)
                     /locus_tag="VGO32_01975"
     CDS             complement(284..949)
                     /locus_tag="VGO32_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7199551.1"
                     /translation="MDPVKAPRRYDASRRREQARRTHDSVLGAARQRLLDHGYADTTV
                     AMIAADAGTSVDTIYKAFGGKAGLVRTLWRQALEGEEPTPAPTRSDQLSQTETDPVRV
                     LHGWGRFTTELAPRAAPILLLVKAAAATDSDMALLLADSEQQRRTRMRHNARRLQRRG
                     WLRAGVTLAQATDILWTYSSAELYDLLVLRSGWPVERYGAFIGDALVAALLPPTEGGS
                     RSD"
     gene            1009..1425
                     /locus_tag="VGO32_01980"
     CDS             1009..1425
                     /locus_tag="VGO32_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7199552.1"
                     /translation="MTDALEVVNRLVAATNAHDLDALVACFDDDYVNETPAHPLRGFR
                     GPAQVGENWRAIFAGIPDISVRVTASARHGNEVWSEWRMDGTRHDGARHTMAGVIIFG
                     VTDDRISSARFYLEPVETTTGDVSTAIGRVVSERAS"
     gene            1422..2276
                     /locus_tag="VGO32_01985"
     CDS             1422..2276
                     /locus_tag="VGO32_01985"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7199553.1"
                     /translation="MILVVGGTGMLGRDLVSRFLSAGHRVRVLTRDRTRAAGLATEVT
                     VGDVLDPASLLEGVRGCDTVVAAMHGFLGGRRAGPEQVDETGTANLVAAAAAEGAPKF
                     VLLSIAGARADHPIGLFRAKYAAEQYLRASALDWTIVRPSAYMQTWAGIVGAQLPDGP
                     ATVFGRGKNPINFVAVDDVAAVVERAATDPTLSRMSVDAPGPQNLTMNQFAEHLGATR
                     IRHIPRSALTVLATAAKPIAPAFARQARTALVMDSLDMTADPAAAHARFPDLRWSRLA
                     EILDTVNR"
     gene            complement(2685..3389)
                     /locus_tag="VGO32_01990"
     CDS             complement(2685..3389)
                     /locus_tag="VGO32_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199554.1"
                     /translation="MSSIRSMKYYADSSSRRQNQILADVLVLIWVVACIWAGWNVYNG
                     IQQVRPAATELSSAGDSIRTNMSSAASGVGSIPFGVGDALRGPFDAISGAGQTLADAG
                     TSLGMTIDEFSRMAGLFVALVPIILVAGPWALIRYRFVRRATNAQQWLTRAGSLEMFA
                     LRALVTLPLDRLEPLGPDVVSGFFRGDQATVRALAALELEAYGVSPSDADETPAIAPG
                     PNAAGPDAPGPDAPAT"
     gene            complement(3495..3659)
                     /locus_tag="VGO32_01995"
     CDS             complement(3495..3659)
                     /locus_tag="VGO32_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199555.1"
                     /translation="MRAGGSHVETLVRDRDAEELFGVNAMDPTSGTAIARAGQEQARA
                     LSEQLAALWY"
     gene            4096..4443
                     /locus_tag="VGO32_02000"
     CDS             4096..4443
                     /locus_tag="VGO32_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199556.1"
                     /translation="MTSPGQWGKNKPRNLQEALAGVRTSLKMIDDAKKPSGAVGAHVA
                     TIEDAAAGLLDVINDSSSGHTVRNRVAQFDAGTRAAKNAVNAEIGRGNMSVGPAAALL
                     GSIDNLRELAQNL"
BASE COUNT          811 a         1676 c         1654 g          729 t
ORIGIN      
        1 cgccgaaaac cgggtgggcg acgccggtgg tgtccgggtt ctcgcggctg aggtggatcg
       61 cctgaacgag gctggacgcg cacgaaccca cacccaccaa ggccgcgcgg atcatcgatc
      121 gttcccctct gtggcactgg atcccgacgt cgtcgagcta cgtaagactg gcacgagcgg
      181 accactgcgc gaaggcttgt ggtgccggta gtgccaaggg ccttgcgtgc ccgagcactc
      241 aggccctcag cttgccgccc cgtgttttgc cggttgggtc tcctcagtcg ctgcgacttc
      301 cgccttcggt tggtggcagc agggcggcga cgagggcgtc accgatgaac gcgccgtatc
      361 gttcgaccgg ccaaccggac ctgagtacca gcaggtcgta gagctcggcc gagctgtagg
      421 tccagaggat gtccgtcgcc tgggcgaggg tgacgccggc acggagccag ccgcggcgtt
      481 gcaggcggcg cgcgttgtgc cgcattcggg tccggcgctg ctgctcggag tcggcgagga
      541 gaagcgccat gtccgagtcg gtcgcggctg cggccttgac gaggagcaag atgggcgccg
      601 cgcggggggc gagctcagtg gtgaaccggc cccagccgtg caggacgcgg acgggatcgg
      661 tctcggtctg gctgagctgg tcggaacggg tcggcgcggg cgtgggctct tcgccttcca
      721 gggcttgtcg ccacagcgtc cggaccaggc cggccttgcc gccgaacgcc ttgtagatcg
      781 tgtcaaccga cgtccccgcg tccgccgcga tcatcgccac cgtcgtgtcc gcatacccgt
      841 ggtcgaggag gcgctgccgg gccgctccca gcacggagtc gtgcgttcgg cgggcttgtt
      901 cgcggcgccg gctggcgtcg tagcgacggg gggccttgac gggatccata ttccgatcca
      961 tagttctaga atgaataagc cgagcgtatc aacctcaggc cggacgcgat gaccgacgcg
     1021 ctggaggtcg tcaaccgact ggtcgccgcg acgaacgccc acgacctgga cgctctggtc
     1081 gcgtgcttcg acgacgacta tgtgaacgag acgcccgcac acccactgcg cgggttccgg
     1141 ggacctgcgc aggtagggga gaactggaga gccatcttcg ccggcatacc ggatatctcg
     1201 gtccgcgtga ccgcgtctgc gcggcacggc aatgaggtct ggtcggaatg gcggatggac
     1261 ggtacccgac acgacggcgc ccgccacacg atggccgggg tgatcatctt cggtgtgacg
     1321 gacgaccgga tatcgtccgc caggttctac ctcgaacccg tcgagaccac gaccggtgat
     1381 gtatccaccg cgatcggtcg cgtggtgtcc gagcgcgcgt cgtgatcctg gtcgtcgggg
     1441 gcacggggat gctcggccga gatctcgtct cccgattcct gtctgctggg caccgggtac
     1501 gggtgctcac gcgcgaccgt acgcgtgcgg cggggctggc cacggaggtc accgtgggcg
     1561 atgtcctcga ccctgccagc ctcctcgagg gggtgcgggg atgcgacact gtcgtcgcgg
     1621 cgatgcacgg attcctcggc ggacgccgcg caggcccgga acaggtcgac gagaccggca
     1681 cggcgaatct cgtcgccgcg gccgccgccg agggcgcccc gaagttcgtg ctcctgtcca
     1741 tcgccggggc ccgcgccgat caccccatcg ggctgttccg ggccaagtac gccgccgaac
     1801 agtacctccg cgccagcgcc ctggactgga ccatcgtgcg gccgagcgcc tacatgcaga
     1861 cctgggcggg gatcgtcggg gctcagctgc ccgacgggcc tgccaccgtc ttcggccgcg
     1921 ggaagaaccc gatcaacttc gtcgcagtgg acgacgttgc cgcggtcgtc gaacgcgccg
     1981 ccaccgatcc gaccttgtca cggatgagtg tggacgcacc cggaccgcag aacctgacga
     2041 tgaaccagtt cgccgaacac ctcggcgcca ccaggatcag gcacatcccc cgatccgctc
     2101 tgaccgttct cgccacggcg gcgaagccga tagcgccggc gttcgcgcga caggcgcgca
     2161 ccgcgctcgt gatggactcc ctcgacatga ccgccgaccc cgcagccgcc cacgcccgct
     2221 tcccggatct gcggtggtct cggctcgcgg agatcctgga caccgtcaac cgttaagccg
     2281 ctcgaaccgg cagcgacgag gtgcgcactg cagcagtcat ggtggcgttc ttctcagccc
     2341 tagaccggcg aataaggccc ctgccccgtt aacggggcag gggccaggtc gcctgtctca
     2401 acacagagcg cgcccgaagg gattcgaacc cccaaccttc tgatccgtag gaaaccggcc
     2461 ggcgccgtgc taccgctcga gcggtccgcc gccgtgagcc tgaggtccca cgtagtgagt
     2521 ggccatcgac ctgcgggaag agccgcgaag ctcgacgaca tcctcggcca ggccctcagc
     2581 ccggccgacg cgtcctccgc caaacccgcg aagccaaccg acccggccgc agtcggcacc
     2641 gtcctcgacg gagccctcgg cggtctgctc ggccacggaa agcgctaggt agcgggggcg
     2701 tccggaccgg gggcgtccgg tccagcggcg ttgggtccgg gggcgatggc cggcgtctca
     2761 tcggcgtcac ttgggctgac gccgtaggcc tcgagctcga gagcggccag cgcgcggacg
     2821 gtcgcctggt ctccgcggaa aaacccgctg accacatcgg gaccgagcgg ctccagccgg
     2881 tccagcggca gcgtgaccag agcccgcagg gcgaacatct ccagcgaccc ggctcgcgtc
     2941 agccactgct gggcgttggt cgcccggcgc acgaaccggt acctgatcag cgcccacggc
     3001 ccggcgacga gaataatggg gaccagcgcc acgaacaggc cagccatacg cgagaactcg
     3061 tcgatcgtca tgcccaggga cgtgcccgca tcggccaggg tctggccggc gccagagatc
     3121 gcgtcgaagg ggccgcgcag ggcgtcgcca acaccgaacg gtatcgatcc gacaccgctg
     3181 gcggcactgg acatgttggt ccggatggag tccccggccg aggaaagctc ggtggccgcg
     3241 gggcgcacct gctggattcc gttgtagacg ttccagcccg cccagatgca cgccaccacc
     3301 cagatgagga ccagcacatc ggcgaggatc tggttctgcc gacgcgagga cgagtcggcg
     3361 taatacttca tcgatcggat cgagctcact gacatcgtcg aactcatgac tggctccctt
     3421 tggtggtcgt cccggtgcga taaccacgct cctccgctga ggaccattgc ggggcaaaaa
     3481 gggcaaaagg gcgttcagta ccaaagcgcg gcgagctgct ccgacaaggc gcgcgcctgc
     3541 tcctggccgg ctcgcgcaat cgccgtcccg gaggtcggat ccatggcgtt gacgccgaac
     3601 agctcctcgg cgtcgcgatc ccggacgagc gtctcgacgt ggctgccgcc cgccctcaac
     3661 tgctcgacct tccgccgcca gccacaaacc tcaggccgcg cacttcgacc gccattgcgg
     3721 cgtcaacctc gtggatgcgc cgcgagcacc ggggagtggc gccgttaccg catcggcgcc
     3781 cgcgcccaaa tacgggtcgg cgacggtgac caccccgcca gacgcccttt gccctcctaa
     3841 cggttgcccc gaggcgtcca gactccggcc acgccgggcc ggccgcggcc tcggccggcg
     3901 cgtccggttt cgttgagttc gggccggtaa cagccgtgga caacggacgc cgagcgcggc
     3961 catcatgccc gtgagggact tgaagacgtc cagctgacca gacggatcac ggacctcgtc
     4021 cggcgctgcc tcctacgacg acctaaccct tactaccacc accaaacaga gggagtcccg
     4081 atgacggaca tgccgatgac cagccccggc cagtggggga agaacaagcc gaggaacctg
     4141 caggaggcgc tcgccggtgt gcggacctcg ctgaagatga tcgacgacgc caagaagccg
     4201 agtggcgcgg tcggcgccca tgtcgccacg atcgaggatg cggccgcggg tctcctggac
     4261 gtcatcaacg acagcagtag tggtcacacg gttcgcaacc gcgttgccca gttcgacgct
     4321 gggaccagag cggcgaagaa cgccgtcaac gccgagatcg gacgcggcaa catgtccgtc
     4381 ggcccggcgg ctgcgctcct cggcagcatc gacaacctcc gagagctcgc gcagaacctc
     4441 tgacgacaga agtcatcacg accttacggc ccagcacaac tgagcttgac cgccagcctg
     4501 gaagagggaa tcttgcaaaa gtgggaccca agcgagtttc gacgggagcg cactcagttg
     4561 gccgagctgg cttccacaca tgtgcgggcc ggcagggcaa ccgcaaacga atgtggcggg
     4621 ccgggctatt cgcccgatgt cacggtgtca accgttcaga tcggctgatc tcccgccggt
     4681 tggcaccttc gagactgcac ctaggcgttt gtgaggatcg ccgcttggcc tcccgggtgg
     4741 cgaggaccga ccgtgcatgc ccggtgcggc cgcggtgatc gaggcggccg tcgccgggga
     4801 agctcgggtg actatcgtcc ctcttcatcg tcgccagcgc gctgggtctg cggtccgtac
     4861 gcagaggaag
//
LOCUS       DASYGU010000080         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1041092, whole genome shotgun sequence.
ACCESSION   DASYGU010000080 DASYGU010000000
VERSION     DASYGU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041092"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VGO32_02005"
     CDS             <1..603
                     /locus_tag="VGO32_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV7199557.1"
                     /translation="ERLQNEFLRLQARLRKTIVFVTHDVDEAIKMADRIVILQVGGIV
                     AQYDTPDRLLAAPASDFVERFVGADRGLKRLSLARVRDLTLEPVITATVGEPRSAVRA
                     RLTDNAGWVLVTDERRHPIGWVDAADLAGDGPVDAAMAVPGSSLLQPESTLRDALSTM
                     LASSAQVGVVVDEREELLGVVSVAAISATMQDGAVPDGGS"
     gene            600..1316
                     /locus_tag="VGO32_02010"
     CDS             600..1316
                     /locus_tag="VGO32_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199558.1"
                     /translation="MNDQPFFRIDWVIDNLDVLAQATIEHIVLTLIAVIGGIAIAFPV
                     TIFIQRRRRLIGPVLGFTGILYAIPSLALFAVLIPFTGIGLLTAEIALISYTLLILIR
                     NGVAGLDGVDPDVLEASTGMGLSRRQRLWRVELPLATPVIMAGLRIAVVTTIGLVAVT
                     ALIGQGGLGFVIIVLGIRRQFETAIVVGVLLSILLAVLADRGLVLLERALTPWTRART
                     AAGPSASSEPPVAGSTLGAS"
     gene            1320..2057
                     /locus_tag="VGO32_02015"
     CDS             1320..2057
                     /locus_tag="VGO32_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199559.1"
                     /translation="MEIVGEVVAWLTDPAHWTGSDGIPARFSEHVVLSGVSLAVGLAI
                     ALPLGLAIGHTRRWAGAVVAITNIGRAVPSVGILGIAFVLTLPLVAALNLRNIGTPAA
                     LIALIALAIPPIVVNAYVGIRDADPDVVEAARGMGMSERQVLRRVEVPLGLGVILAGI
                     RTASIQVIATATLGAILSTGGLGRYIVDGFAQRDYPQMVSGALLVMVLVFSSEAIFAL
                     VQRRTVSPGLAIDRTDRLPEPAPGFGL"
     gene            2161..>2827
                     /locus_tag="VGO32_02020"
     CDS             2161..>2827
                     /locus_tag="VGO32_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7199560.1"
                     /translation="MRKTRMLAAGAALVLLVSACQPGGSESPSPSAPSSGAPSTQAEL
                     PTVIVGSANFDEAAVLAEIYAQALEAAGFTVERNLYTGPRETTLPALESGELNLMPEY
                     IGSLLSVGLGGEASGDPETTFADLQDALAEVGLVAFDFSEAADQDAFAVTSETAKAND
                     LVTVTDVAPIAADLTWGLPPECETRPTCGIGLNDLYGIDMAAIPVETIDPCGPAMAEA
                     LNTG"
BASE COUNT          387 a         1020 c          960 g          460 t
ORIGIN      
        1 gagcggctcc agaacgagtt cctccgcctc caggcccggc tccgcaagac gatcgtgttc
       61 gtgacccatg acgtggacga ggcgatcaag atggccgacc ggatcgtcat cctgcaggtc
      121 ggcgggatcg tggcccagta cgacacgccc gatcgactgc tcgccgcgcc ggccagcgac
      181 ttcgtcgagc ggttcgtggg ggccgaccgg ggtctgaagc gcctctccct cgcccgcgtg
      241 cgcgacctca ccctggagcc ggtcatcacc gcgaccgttg gcgagccgcg atccgcggtc
      301 cgggcccgtc tgaccgataa cgccggctgg gtcctcgtca ccgacgagcg gcgccacccg
      361 atcggctggg tcgacgcggc cgacctcgcc ggcgacggtc cggtggatgc ggcgatggcc
      421 gtgccgggct cgtcgctcct ccagccggag tccaccctcc gcgacgcgct gtccaccatg
      481 cttgcgtcgt cggcccaggt cggggtggtg gtcgatgaac gcgaggagct gctcggcgtg
      541 gtcagcgtgg cggccatctc ggccacgatg caggacggcg ctgtgccgga cggagggtca
      601 tgaacgacca gccgttcttc cggatcgact gggtcatcga caacctcgac gtgctcgcgc
      661 aggccaccat cgagcacatc gtcctgaccc tgatcgcggt catcggcggg atcgccatcg
      721 ccttcccggt gaccatcttc atccaacgcc gccggcggct catcggcccg gtgctgggct
      781 tcaccgggat cctgtatgcc atcccgagcc tggccctctt cgccgtgctc atcccgttca
      841 ccggcatcgg cctgctcacc gccgagatcg cgttgatcag ctacaccctg ctcatcctca
      901 tccgcaacgg ggtggcgggc ctggacggcg tggaccccga cgtcctcgag gcgtcgaccg
      961 ggatgggcct cagccgacgg cagcgcctgt ggcgggtcga gctgccgctg gcgaccccgg
     1021 tgatcatggc cggactccgg atcgcggtgg tgaccaccat cggcctggtg gcggtgaccg
     1081 ccctcatcgg ccagggcggg ctgggcttcg tgatcatcgt cctgggcatc cggcgccagt
     1141 tcgagaccgc gatcgtcgtc ggcgtgctgc tgtctatcct gctggcggtc ctcgctgatc
     1201 gcggcctcgt ccttcttgag cgggcgctca cgccgtggac ccgagcccgc accgccgccg
     1261 gtccgtccgc gtcgtccgaa cctccggtgg ccggttcgac gctcggggcg agctgagcca
     1321 tggagatcgt cggcgaggtc gtggcctggc tcacggatcc ggcccactgg accggatcgg
     1381 atggcatccc ggcgcggttc agcgagcacg tcgtcctgtc cggcgtctcc ctggcggtgg
     1441 gcctcgccat cgccctgcct ctcgggctgg caattggcca cacgcggcgc tgggcgggag
     1501 cggtggtcgc gatcacgaac atcgggcggg ccgtcccctc ggtgggcatc ctgggcatcg
     1561 ccttcgtcct gaccctgccg ctggtcgccg ccctcaacct gcgcaacatc ggaacgccgg
     1621 cggcgctcat cgcgctcatc gcgcttgcca tcccgccgat cgtggtcaac gcgtatgtcg
     1681 gtatccgcga tgcggacccc gacgtggtcg aggccgcccg gggcatgggc atgagcgagc
     1741 gacaggtcct ccgacgggtg gaggtgccgc tgggcctggg cgtcatcctc gcggggatcc
     1801 gcaccgcatc gatccaggtc atcgccaccg ccaccctggg cgccatcctg tccaccggtg
     1861 gcctggggcg ctacatcgtg gacggcttcg cccagcggga ctacccgcag atggtgagtg
     1921 gggcgctgct ggtcatggtc ctggtgttca gcagcgaggc catcttcgcc ctcgttcagc
     1981 ggcgaacggt ctcgccagga ctcgcgatcg atcggacgga ccgcctcccg gagcccgcgc
     2041 ccggcttcgg cctctaacca gcgtaccggc tacactggta actggtagac tgcccccacg
     2101 gtccgcgtcc agcggcccag gtcggcgcga gccgatgaag atccaccaga ggaggaccca
     2161 atgcggaaga cccgcatgct tgctgctggg gcggctctgg tactgctcgt cagtgcctgt
     2221 cagccgggtg gcagcgaaag cccaagcccg tcagctccga gttccggcgc gccctcgacc
     2281 caggccgaac tccccaccgt catcgtcggc tccgccaact tcgacgaggc ggccgtgctc
     2341 gccgagatct atgcccaggc cctcgaggcg gctggattca cggtcgagcg caacctgtac
     2401 accggaccgc gggagaccac cctcccggcg ctcgagagtg gcgagctgaa cctcatgccc
     2461 gagtacatcg gcagcctgct gagtgttggg ctcggcggtg aggcgagcgg cgaccctgag
     2521 accacgttcg ccgacctgca ggacgccctg gccgaggtgg gcctggtggc gttcgacttc
     2581 agcgaggcgg ctgaccagga tgcgttcgcg gtcacctccg agaccgccaa ggcgaacgac
     2641 cttgtgaccg tcaccgacgt cgcgccgatc gcggccgatc tcacctgggg cctgccgccc
     2701 gagtgcgaga cccgaccgac gtgcgggatc ggcctgaacg acctgtacgg gatcgacatg
     2761 gcggccattc cggtcgagac catcgacccg tgtgggcctg ccatggccga agcgctcaac
     2821 acgggcg
//
LOCUS       DASYGU010000081         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_886847, whole genome shotgun sequence.
ACCESSION   DASYGU010000081 DASYGU010000000
VERSION     DASYGU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886847"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGO32_02025"
     CDS             <1..499
                     /locus_tag="VGO32_02025"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988654.1"
                     /note="E3 component of alpha keto acid dehydrogenase
                     complexes LpdC; forms a homodimer; binds one molecule of
                     FAD monomer; catalyzes NAD+-dependent oxidation of
                     dihydrolipoyl cofactors that are covalently linked to the
                     E2 component; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV7199561.1"
                     /translation="TGVPHLYAIGDVVPGYALAHVASHESIVAVETIAGLDVEPVRMD
                     LMPRVTFCRPEIASVGLSEAEAAAAGTSVKVGSFPFRALGKATIVGEVDGFAKFVADA
                     TTGRLLGAHIIGPHAGDLLAEPTFARLVEGTAEEIAMSVHAHPTLTEVLAEAALAVDG
                     AALHV"
     gene            503..1612
                     /locus_tag="VGO32_02030"
     CDS             503..1612
                     /locus_tag="VGO32_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV7199562.1"
                     /translation="MTDADAETKVRRVLGLDTTNGQADGSPPTPATNTAPSADTAPHR
                     ALGLTDEDVLAMYRQVLIARAVDERMWLMQRAGKIAFIISVQGHEVAQVGTAWPMRKG
                     QDWMAPFYRSIAAAMTFGMTPEDIISAHLAKGDDISSGGRQMPGHYGAAPHNILSVSS
                     PVGTQVLHAVGLAMGAWVRGDDVVAVTQFGEGTANQGEIHEAMNFAGVHKLPVVFICE
                     NNGYAISVPLERQVGGGSVAARAAGYGFPGVEVDGGDLLACYAAGREAHERARTGGGP
                     TLIEAHVVRLTSHSSDDDQRRYRDAADVEALKERDPIPRLAASLREWGVLDDAGEEAL
                     KAEVKTLIAESAARAEARPEPDATTILRHVYADGS"
     gene            1614..>2441
                     /locus_tag="VGO32_02035"
     CDS             1614..>2441
                     /locus_tag="VGO32_02035"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV7199563.1"
                     /translation="MAELNILEAIRRAMDEELAADERVMVLGEDVGRKGGVFGATDGL
                     WAKYGDKRVLDTPLSEGLIAGAAMGAALYGLRPIAEMQFADFVYPAFNQIVSEIARMR
                     FRSNGAFGVPMVIRMPYGGGVHGALYHSQSNETYFTSTVGLKVVAPGTPADAYGLLKA
                     AIRDPDPVMFFEHKKTYRLFKAEVPDDGDGVVPIGSAVVARPGTDLSIYCYGYMRYCA
                     LEAAETLAGEGIAAEVVDLRTLRPLDTETVLASVTKTGRALVVHEANQFGGFGAEVAA
                     "
BASE COUNT          377 a          834 c          879 g          351 t
ORIGIN      
        1 caccggcgtt ccgcacctgt acgcgatcgg tgacgtcgtc cccggctatg ccctcgccca
       61 cgtcgccagc cacgagtcga tcgtggcggt cgagaccatc gccggcctgg acgtggaacc
      121 ggtccggatg gacctcatgc cgcgggtcac gttctgccgg cccgagatcg cgagcgtggg
      181 tctgagcgag gccgaagcgg cggcggccgg cacctcggtc aaggtcggca gcttcccgtt
      241 ccgggcgctg ggcaaggcca cgatcgttgg cgaggtcgat ggcttcgcga aattcgtggc
      301 cgacgccact accggccggc tgctcggggc gcatatcatc gggccccacg ccggcgacct
      361 gctggcggag ccgaccttcg cccgcctggt ggaggggacg gccgaggaga tcgcgatgtc
      421 ggtgcatgcc catccaaccc tgaccgaagt cctggccgag gccgccctgg cggtcgacgg
      481 cgcggccctc cacgtctgac cgatgaccga cgctgacgcc gagaccaagg tccgccgcgt
      541 gctcggcctg gatacgacca acggccaggc cgatggctcg ccgccgacgc ctgcaacgaa
      601 cacggcgcca tccgcggaca cggccccgca tcgggccctc ggactgaccg acgaggacgt
      661 gctggccatg taccgccagg tgctcattgc ccgcgcggtg gacgagcgga tgtggctcat
      721 gcagcgagcc ggcaagatcg cgttcatcat cagcgtccag ggccacgagg tggcgcaggt
      781 cggcaccgcg tggccgatgc gcaagggcca ggactggatg gcgccgttct atcggtccat
      841 tgcggcggcc atgaccttcg gcatgacccc ggaggacatc atcagcgccc acctggccaa
      901 gggcgacgac atctcgtccg gcgggcggca gatgcccggc cactacggcg cggccccgca
      961 caacatcctc agcgtcagct caccggtcgg aacccaggtc ctccatgcgg tgggcctggc
     1021 catgggggcg tgggttcggg gtgatgacgt ggtcgcggtc acccagttcg gcgagggcac
     1081 ggcgaaccag ggcgagatcc acgaggccat gaacttcgcg ggcgtccaca agctgccggt
     1141 cgtgttcatc tgcgagaaca acggctatgc gatcagcgtt cccctcgagc ggcaggtggg
     1201 cggcggttcg gtcgcggcca gagccgcggg ctacggattc ccgggcgtgg aggtggacgg
     1261 cggggacctg ctggcctgct acgcggccgg ccgcgaggcc cacgaacggg cccggaccgg
     1321 tggcggtccg accctcatcg aggcccacgt cgtgcgcctc acctcgcact cctccgacga
     1381 tgaccagcgc cgctaccgcg acgccgccga tgtggaggcg ctgaaggagc gcgatccgat
     1441 cccccgcctg gcagccagcc tgcgcgagtg gggcgtgctg gacgatgcgg gggaagaggc
     1501 gctcaaggcc gaggtcaaga ccctgatcgc cgagtctgcg gccagggccg aggcgcggcc
     1561 cgagcccgac gccaccacca tcctgcgcca cgtgtacgcg gacggttcct gatatggccg
     1621 agctgaacat cctcgaggcc attcgtcggg caatggatga agagctggcc gccgacgagc
     1681 gggtcatggt cctgggcgag gacgtgggcc ggaagggcgg cgtgttcggc gccacggacg
     1741 gactgtgggc gaagtacggc gacaagcgcg tgctggacac cccgctcagc gaggggctca
     1801 ttgccggcgc ggcgatgggc gcggcgctgt acggactgcg gccgatcgcc gagatgcagt
     1861 tcgcggactt cgtctacccc gccttcaacc agatcgtcag cgagatcgcc cgaatgcgct
     1921 tccggtccaa cggggcgttc ggggtaccga tggtcatccg catgccgtac ggcggcggtg
     1981 tccacggtgc gctgtatcac agccagtcga acgagaccta cttcacgtcc accgtgggcc
     2041 tgaaggtggt cgcgccggga accccggccg acgcctacgg cctcctgaag gctgccatcc
     2101 gcgacccgga cccggtgatg ttcttcgagc acaagaagac gtatcggctg ttcaaagccg
     2161 aggtgcccga tgatggcgac ggagtggtgc cgatcggctc ggcggtggtc gcccggccgg
     2221 gcaccgacct gtcgatctac tgctacggct acatgcgcta ctgcgcgctc gaggcggcgg
     2281 agaccctggc cggggagggc atcgcggccg aggtcgtgga cctgcgcacc ctgcggcccc
     2341 tggataccga gactgtcctc gcctcggtca cgaagaccgg tcgcgcgctg gtcgtccacg
     2401 aggccaacca gttcgggggc ttcggggcgg aggtcgccgc g
//
LOCUS       DASYGU010000082         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1247210, whole genome shotgun sequence.
ACCESSION   DASYGU010000082 DASYGU010000000
VERSION     DASYGU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247210"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGO32_02040"
     CDS             complement(<1..270)
                     /locus_tag="VGO32_02040"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7199564.1"
                     /translation="MTFQSADSLATRDLRLLRDLYRALADPTRLRVCAILGESGPLPV
                     NELGSRFGLSQPLISWHLRILRTAGLVETRPHGREKLCALRPGAFE"
     gene            405..1208
                     /locus_tag="VGO32_02045"
     CDS             405..1208
                     /locus_tag="VGO32_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199565.1"
                     /translation="MPISTPDALWSSPAARRLRPVAIEIEGLARRVEGVPLLDGVDLA
                     IPAGARVLIAGVAPESPSLLLRIMAGLARPDRGRVVLAGLHRSATIPEGWARRIGFVG
                     SDPGIPLWMTPAESLQLAARLAGIDGPQRDRLIDESLQRYQLGAIRDRPLRHAGRIIA
                     ERTALAAALLPDPEVLLLDEPLRAHSPPDRLRLLRIPGERRTVLIASQYPAQEGGIVD
                     RIVLIRDGRIALHAPITELDAQRLPLSLRGMTALADLVMAGRPTEDPES"
     gene            1205..>1520
                     /locus_tag="VGO32_02050"
     CDS             1205..>1520
                     /locus_tag="VGO32_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199566.1"
                     /translation="MILGVHVIRQLWIQLRLLGLLALPPLAAIVAVIVQGQLGPVAGP
                     QPARLTLAVGFAVAAVLSAALIGTGFSEEVRSGAAAWLVVRAVPRTALIGAWLVVPSL
                     AVV"
BASE COUNT          189 a          537 c          550 g          244 t
ORIGIN      
        1 ctcgaacgcg ccggggcgca gggcgcacag cttctcccgg ccgtgtggcc gggtctccac
       61 caggccggcc gtgcgcagaa tgcgcagatg ccagctgatg agcggctgcg acaagccgaa
      121 ccgggacccg agctcgttga cgggcagtgg cccggactcg cccaggatcg cgcacacccg
      181 caggcgggtg gggtcggcga gcgcccggta caggtcacgg agcagccgca ggtcgcgcgt
      241 cgcgagggag tcggcggact ggaaggtcat gtcgtgggaa atcctcgtga gagcctgggg
      301 aggctcaagg gtgtctttat ataaggcgcc gcttatggta gcgcgggatt ctcggatgtc
      361 aagtggattt ctgggcctcg gccaacacgc tagagtcgcc tgagatgccg atctccacgc
      421 ccgacgccct gtggtcatcg ccggccgcgc gccgtctgcg gccggtggcc atcgagatcg
      481 aggggctggc tcgacgggtg gagggtgtac cgctgctcga cggtgtggat ctggcgatcc
      541 ccgccggcgc gcgcgtgctc atcgccggcg tggccccgga gagtccatcc ctgctcctcc
      601 ggatcatggc gggcctcgcc cgtcccgatc ggggccgggt cgtcctggcg gggctccacc
      661 gatcggcgac catccccgag ggctgggcgc gacgaattgg gttcgtcggg tcggaccccg
      721 gcattccgct gtggatgaca ccggccgaga gcctgcagct ggcagcgcgg ctggccggga
      781 tcgacggccc gcagcgcgac cgtttgatcg acgagtcgct gcagcggtac cagctgggcg
      841 cgatccggga ccgacccctc cgccacgcgg gtcgaatcat cgccgagcgg accgcgttgg
      901 cggccgccct gctccccgat cccgaggtcc tgctcctcga tgagccactc cgcgcccact
      961 ccccgccgga ccggttgcgg ttgcttcgga tcccggggga gcgacgaacg gtgcttatcg
     1021 ccagccagta cccggcccag gagggcggca tcgtggaccg gatcgtgctc attcgcgatg
     1081 gccggatcgc gcttcacgcg ccgatcaccg agctggacgc gcagcgcctc cccctgtcgc
     1141 tgcgcgggat gacggccctc gcggacctcg tgatggccgg ccgccccaca gaggaccccg
     1201 aatcgtgatc ctgggcgtcc atgtcattcg ccagctgtgg atccagctgc ggctgcttgg
     1261 tctcctggcc ctgcctcccc tcgccgcgat cgtggccgtg atcgtgcagg gccagctcgg
     1321 cccggtcgcc ggcccccaac ccgcccggct gacccttgcc gttggcttcg cggtggccgc
     1381 cgtgctgagc gccgccctga tcgggaccgg tttcagcgag gaggttcggt ccggcgccgc
     1441 ggcatggttg gtcgtccggg cggtcccgcg taccgcgctc atcggcgcct ggctcgtggt
     1501 gcccagcctg gccgtggtcg
//
LOCUS       DASYGU010000083         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_630017, whole genome shotgun sequence.
ACCESSION   DASYGU010000083 DASYGU010000000
VERSION     DASYGU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630017"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..703
                     /locus_tag="VGO32_02055"
     CDS             266..703
                     /locus_tag="VGO32_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199567.1"
                     /translation="MNCQPIDMSLPSGEPLDLTGSWEGNDLGPYQLRQFGDCLWWVGQ
                     NNILSVLFFGHLRTDFTVRGRWATIAASDHDVGGTRNSADLYVGSGVLVLDVVVGEDG
                     TNADVSLTRTAIIDSPDFGPGYEYGVTTWTRVDDTPDHPIPGT"
     gene            763..1539
                     /locus_tag="VGO32_02060"
     CDS             763..1539
                     /locus_tag="VGO32_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV7199568.1"
                     /translation="MRLPLFPLHVVAFPHLPLPLHIFERKYRQMTADLLAEGNEYGGR
                     FVVTMISAGREVASKPGTGQLTVRRVGTICEIRRADRFADGRYALLSVGVGRARIRKV
                     DHSGLYATADVTPLDERRGEDAESLVPNVQVALDGYLATIRGFLADRDAAEMEDLLAD
                     AEFDIDPDLLHESSAGDVKRVTRMDRLITPIKLPSDPLAASYAVAGVLQVELSRKQRL
                     LEAPDAAARLRAELAILQREAQLLGTASLAPMGRFEYHAN"
BASE COUNT          251 a          572 c          541 g          275 t
ORIGIN      
        1 aaggcgaccg gcaacggcca ccgcgagatc gggcagtccg cactcgactt ctacaccgag
       61 tggaagagcg tctacatcga ctattccggg aagctccagc gggcccagat cgacatggag
      121 ttcgtcgatc aggactaggg ccctggccgt cctggcggtt gcggggatgg tgatcgcctg
      181 ttcgccgtcg gcctcggaac ctggttcggc gactccgtcc gcaatcgcgc aggcctcgcc
      241 cggatcggtg gcctccgagg atgaggtgaa ctgccagccc atcgacatga gcttgccgtc
      301 tggtgagccg ttggacctca cgggaagctg ggaggggaac gacctgggtc cgtatcagct
      361 gcgccaattc ggcgattgcc tgtggtgggt cggccagaac aacatcctgt ccgtcctgtt
      421 cttcggacac ctgcggaccg atttcacggt gcggggtcga tgggccacga tcgcggccag
      481 cgatcacgac gtgggtggca cgcgaaattc agccgacctg tatgtcgggt cgggcgtcct
      541 ggtcctggac gttgtggtcg gcgaggacgg gaccaacgcg gacgtctcgc tcacgagaac
      601 cgcgatcatc gattccccgg acttcgggcc cggctacgag tacggcgtca ccacctggac
      661 gcgggtcgac gacacgcccg atcacccgat cccgggcacc tgaggcggct gcccaaatgg
      721 gctaatgggc cacatcggcc cttcggagta cgatgacggc ccatgcggct gcccctgttc
      781 ccgctccatg tcgtggcctt cccgcacctg ccgcttccgc tccacatttt cgagcgcaag
      841 taccggcaga tgaccgctga cctcctcgcc gagggcaatg agtacggcgg gcgattcgtg
      901 gtgaccatga tctcggccgg ccgcgaagtg gcatccaagc cgggcaccgg ccagctcacc
      961 gttcggcggg tgggaacgat ctgcgagatc cgccgtgccg accggtttgc ggacgggcgc
     1021 tacgccctcc tgagcgtggg cgtgggccgg gcgcgcatcc gcaaggtcga ccattcgggg
     1081 ctgtatgcga ccgccgacgt gacgccgctc gacgagcgac ggggcgagga tgcggagtcg
     1141 ctcgtcccga acgtccaggt cgcgctcgat ggctacctgg ccaccatccg tggattcctc
     1201 gccgatcggg acgcggccga gatggaggac ctcctggccg acgccgagtt cgacatcgac
     1261 ccggacctgc tccacgaatc gtcggccggc gatgtgaagc gggtgacccg gatggatcgg
     1321 cttatcaccc ccatcaagct tccgtcggac ccgctggccg ccagctacgc ggtcgcgggc
     1381 gtcctccagg tcgagctgtc acgcaagcag cgactgctcg aggcgcccga tgcggccgcc
     1441 cgtctccgcg ccgagctggc catcctgcag cgcgaagcac agctcctggg caccgcctcc
     1501 ctcgccccga tgggccggtt cgagtaccac gccaactaga ccgatcccgg acggcctccg
     1561 tcgtccggcc gcgttcctcc tcgccgtctt cctcgcggtg gccggctgcg cctcaccatc
     1621 ggcctcgccg tctccctcg
//
LOCUS       DASYGU010000084         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_527541, whole genome shotgun sequence.
ACCESSION   DASYGU010000084 DASYGU010000000
VERSION     DASYGU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527541"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGO32_02065"
     CDS             <1..404
                     /locus_tag="VGO32_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7199569.1"
                     /translation="QEGFVRAWRALPSFRSGAEPRPWMLRIVANAARNRRRGSSRRSA
                     LGLRLAEDPLSDGAAPSPEGAVLAAERREALLAALDRLSADDRLVIGLRWFVELDEAT
                     MAAALDVPRGTVKSRLSRAMQRLRAEVGTDV"
     gene            397..1179
                     /locus_tag="VGO32_02070"
     CDS             397..1179
                     /locus_tag="VGO32_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199570.1"
                     /translation="MSDRFDLDQALDAALTDLSTAIGFPPTPAMASAVANEVRSIARD
                     QAPRWRPTSLPYRRAMLLATAAVLILVGAVGAIGIGIGAIQIRFADGSPLPTPLASVP
                     NRGFGQPATLADAQDAVPFDVRVSADPALGEPDAVYLAAVPEGGTVTLAWGERPGYPV
                     DARGVGLVVTEFAADIGPDTFEKMILEGTSVEPVTVNGRPGWWIEGGVHAFFYRDASG
                     RMVDTTLRLVSSALIWEEDGLALRVEGAPDLAAAMRAAASLE"
     gene            1255..2418
                     /locus_tag="VGO32_02075"
     CDS             1255..2418
                     /locus_tag="VGO32_02075"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV7199571.1"
                     /translation="MKIAVPRETAEGEARAALTPQIAGQLVASGNEVLVQSGAGDAAH
                     FGDAAYADAGATIVPDVAALYGQADLVLRVGRPTNEEIDMLRPDAVLIGTLGTLSNPA
                     LAERLAERGITAISMDAIPRITRAQSMDSLSSQSTVGGYKAVLIAANRLPKFFPLLTT
                     AAGTVRPARGLIMGAGVAGLMAIGTARRLGAVVEAFDVRPVVKEQVESLGATFVEVPM
                     TDEEKASAQTTGGYAREMNEDYQRRQSELIAERVQAADFVITTALIPGRPAPKLITAE
                     MVRTMQPGSVIVDMAAEAGGNCELTEPGREVVVDGVTIIGLTNLPATVPTHASQMYAK
                     NVQTLVDHLVHEGKLTLDLEDEITKGATITHGGKIVHEATAAALAARSGDAKP"
     gene            2415..2753
                     /locus_tag="VGO32_02080"
     CDS             2415..2753
                     /locus_tag="VGO32_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV7199572.1"
                     /translation="MTEALDPIALVDALFILVLAAFVGYEIVSKVPTMLHTPLMSGAN
                     AVHGVIVVGAIIVTATAESPLQLGLGLVGIILGTINVVGGFVVTDRMLEMFRGRRDGG
                     RGEDSSGDKA"
     gene            2750..>3354
                     /locus_tag="VGO32_02085"
     CDS             2750..>3354
                     /locus_tag="VGO32_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV7199573.1"
                     /translation="MTLLELATYAVYLVASVSFILALKFLASPRTARTGNLLGAGGMA
                     LVVAWTIATEPGLLDNLWVLVVGGLVGTVIGVIGARRVPMTAMPQMVAIFNGAGGGAA
                     AVVAVSEFLTATSESGGELLPTIPVIATLLGAVIGSLSLTGSVIAFGKLQGFIAARPV
                     TYPGSRFVAAALLAALLIMAVALAFVTNDVTLFLVFVALAL"
BASE COUNT          463 a         1208 c         1157 g          526 t
ORIGIN      
        1 cccaggaagg gttcgtgcgc gcgtggcgcg ccctgcccag cttccggagc ggggccgagc
       61 cgcggccgtg gatgctccgg atcgtggcca atgccgcgcg aaaccgacgg cgtggctcgt
      121 cccggcggtc ggccctcggc ctccgcctgg cggaggaccc cctgtcggac ggtgcagccc
      181 catcgcccga gggggcggtc ctcgccgctg agcgtcgcga ggcgctgctg gcggccctcg
      241 accgcctgtc cgccgacgac cggctggtca tcggactgcg ctggttcgtg gagctggacg
      301 aggccaccat ggctgccgcc ctcgacgttc cccgcgggac ggtgaaatcc cgcctgtcgc
      361 gggccatgca gcggttgcgg gcggaggtgg ggaccgatgt ctgaccgatt cgacctcgac
      421 caggccctcg acgccgctct caccgacctg tcgaccgcga tcggcttccc gccaacgccg
      481 gccatggcgt ccgctgtggc caacgaggtt cggagtatcg cccgcgatca ggcgccgcga
      541 tggcgaccca cgagccttcc gtaccgccgc gcgatgctcc tggccaccgc ggccgtcctg
      601 atcctggttg gtgcagttgg tgccatcgga atcgggatcg gcgcgatcca gatccgattc
      661 gccgatggca gtccgctgcc gacgccactc gcgtcggtgc ccaaccgcgg cttcggccag
      721 ccggcgaccc tggccgacgc ccaggacgcc gtcccgttcg acgtgcgcgt ctccgctgac
      781 ccggctctcg gcgagccgga tgcggtgtac ctggccgccg tcccggaggg tgggaccgtg
      841 accctggcct ggggcgaacg gcccggctac ccggtcgatg cgcgcggggt cggcctggtc
      901 gtgaccgagt tcgcggcgga tatcggtccg gataccttcg agaagatgat cctggagggc
      961 acgagcgtcg agccggtcac cgtcaacgga cgaccggggt ggtggatcga gggtggcgtc
     1021 cacgccttct tctaccgcga cgcgagcggc cggatggtgg acaccacgct ccgcctagtg
     1081 agctccgccc tcatctggga ggaggatggg ctcgcgctcc gtgtggaagg ggccccggac
     1141 ctggctgccg ccatgcgcgc ggccgcgtcg ctggagtaga ctctcgcccg gccgtggccc
     1201 cgatcgggcg cggtcgtctc ttctgtccac ccgcgctctg ggtcctgccc cgccatgaag
     1261 atcgccgtcc cgcgcgagac cgccgaaggc gaagcgcgtg ccgccctcac gccgcagatc
     1321 gccggccagc tcgtggcgtc cggcaacgag gtgctggtcc agtccggggc cggcgacgcc
     1381 gcacacttcg gcgacgcagc ctacgccgac gcgggcgcca ccatcgtgcc cgacgtggcc
     1441 gccctctacg ggcaggcgga cctcgtgctc cgagttggcc gcccgaccaa cgaggagatc
     1501 gacatgctcc ggccggacgc agtcctgatc gggacgctgg gaacgctttc caatcctgca
     1561 ctggccgagc ggctcgccga gcgcggcatc acggcgatca gcatggacgc cattccgcgc
     1621 ataacccgcg ctcagtccat ggattcgctg tccagccaga gcacggtcgg cggctacaag
     1681 gcggtcctga tcgccgcgaa ccggctgccc aagttcttcc cgctgctgac gaccgcggcc
     1741 gggacggtcc gtccggcccg aggactgatc atgggcgccg gcgttgccgg actgatggcc
     1801 atcggtacag cccgccgtct gggcgcggta gtcgaggctt tcgacgtccg tcccgtggtc
     1861 aaggagcagg tcgagagcct gggcgcgacc ttcgtcgagg ttccgatgac cgacgaggag
     1921 aaagcctccg cccagacgac cggcggctac gcccgcgaga tgaacgagga ctaccaacgg
     1981 cggcagtccg agctcatcgc ggagcgcgtg caggccgccg acttcgtgat caccaccgcc
     2041 ctgatccccg gccggccggc gccgaagctg atcaccgccg agatggtgcg gaccatgcag
     2101 ccgggcagcg tgatcgtgga catggccgcc gaagccggcg gcaactgcga gctgaccgag
     2161 ccgggtcgag aggtggtcgt ggacggggtc acgatcatcg gcctcacgaa cctgccggcc
     2221 accgtgccca cccacgccag ccagatgtac gccaagaacg ttcagaccct cgtcgatcac
     2281 ctcgtccatg agggcaaatt gaccctggat ctcgaggacg agatcaccaa gggggcgacg
     2341 atcacccacg gcggcaagat cgtccacgaa gcgaccgcag ccgcgctggc cgcccggtcg
     2401 ggagacgcga agccatgacc gaggccctcg atcccatcgc cctggtcgac gcgctgttca
     2461 tcctcgtgct ggccgcgttc gtcggctacg agatcgtgag caaggtcccg accatgctcc
     2521 acacaccgct catgtcgggc gccaacgcgg tccatggcgt gatcgtggtc ggcgcgatca
     2581 tcgtgaccgc aaccgccgag tcgccgctcc agctgggcct cggccttgtc gggatcatcc
     2641 tcggtacgat caatgtggtc ggcggtttcg ttgtcaccga ccggatgctc gagatgttcc
     2701 gcggccggcg ggacggcggt cgcggcgaag actcgtcggg ggacaaggcg tgaccctgct
     2761 cgagctggcg acctacgccg tctacctcgt cgcctcggtc agcttcatcc tggccctgaa
     2821 attccttgcg tcgccccgca ccgcacggac cgggaacctg ctgggggcgg gcggcatggc
     2881 cctcgtcgtg gcatggacca tcgccaccga gcccggcctg ctcgacaacc tgtgggtgct
     2941 ggtcgtgggt gggttggtgg gcaccgtgat cggcgtcatc ggcgcgcgcc gggtgccgat
     3001 gaccgcaatg ccccagatgg tcgccatctt caacggcgcc ggcggtggcg ccgctgccgt
     3061 ggtggccgtg tcggagtttc tgaccgccac cagcgagtcc ggcggggagc tgctgcccac
     3121 catcccggtc atcgccaccc tcctgggcgc ggtgatcggc tcgctgtcgc tgaccggatc
     3181 ggtcattgcc tttggcaagc tgcagggctt catcgccgcc cgcccggtga cctacccggg
     3241 ctcccggttc gtggccgcgg ccctgctggc cgcgctcctg atcatggccg tggccctggc
     3301 cttcgtgacc aacgacgtca ccctgttcct cgtcttcgtg gccctggccc tgat
//
LOCUS       DASYGU010000085         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_630416, whole genome shotgun sequence.
ACCESSION   DASYGU010000085 DASYGU010000000
VERSION     DASYGU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630416"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /gene="tsf"
                     /locus_tag="VGO32_02090"
     CDS             <1..438
                     /gene="tsf"
                     /locus_tag="VGO32_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV7199574.1"
                     /translation="YIHHGSRLGVLLELNCETDFVARTDDFQQLARELAMQVAGLSPE
                     WVSREDVPAEIVAAQREKFAEEAAASGRPADRVDEIVQGKLDKWFESVCLLELPYRDT
                     DRRIGDLITEKVGILGENIRVARFARMAVGESVADQDGTSPQA"
     gene            435..1178
                     /gene="pyrH"
                     /locus_tag="VGO32_02095"
     CDS             435..1178
                     /gene="pyrH"
                     /locus_tag="VGO32_02095"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV7199575.1"
                     /translation="MSSHPAAGDEPRRALLKLSGEALMGARPYGIDREVAQRIAAEAA
                     SAHGEGVQVAIVVGGGNIFRGLAAASTGMDRSTADYMGMLATVMNGLALQDALEQAGA
                     PTRVMSAIAMNEICEPYIRRRAMRHLEKGRVVIMVAGTGNPYFTTDTAATLRAVEINA
                     EVILKATRVDGVYSADPEQDPSAKRYAQIGYTEVLSNRLQALDATAVSLAMDNRMPIV
                     VFDMTVDGNILRALRGESIGTLISDEVPA"
     gene            1175..1732
                     /gene="frr"
                     /locus_tag="VGO32_02100"
     CDS             1175..1732
                     /gene="frr"
                     /locus_tag="VGO32_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV7199576.1"
                     /translation="MSHQALQDVEPKMQRAVEAMERDFAGIRTGRASSAIVERITVEY
                     YGTETPLNQIAGISTPDAHLIVIQPWDRSALPAIEKAITKSDIGLTPNVDGTVVRLHV
                     PPLSEERRHELVKQVRRRLEDARVEIRNFRREAADALKHALKDGSLGQDEEHRELEAL
                     QRLTDGFVEMAEARGERKEAEIMEV"
     gene            1729..>1841
                     /locus_tag="VGO32_02105"
     CDS             1729..>1841
                     /locus_tag="VGO32_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV7199577.1"
                     /translation="MNDLSTTEGPRHVAIIMDGNRRWAASRGLPGIAGHVQG"
BASE COUNT          321 a          613 c          655 g          252 t
ORIGIN      
        1 tacatccacc acggatcgcg actcggggtc ctgctcgagc tgaactgcga gacggacttc
       61 gtggcccgga ccgacgactt ccagcagctc gctcgcgagc tcgcgatgca ggttgccggc
      121 ctgtcgccgg agtgggtctc gcgcgaggac gtcccggccg agatcgtggc tgcccagcgc
      181 gagaagttcg ccgaggaggc ggccgccagt ggccgtcccg cagaccgagt cgacgagatc
      241 gtccagggca agctggacaa gtggttcgaa tcggtctgcc tgctcgagct gccataccgc
      301 gacacggatc gccggatcgg cgacctgatc accgagaagg tcggcatcct gggcgagaac
      361 atccgcgtcg cgcggttcgc tcggatggcg gtgggggaga gcgtcgcgga ccaggacggc
      421 acgtcgccgc aggcgtgagc tcgcaccccg cggccggcga cgagcccagg cgggcgctcc
      481 tcaagctgtc gggcgaggcc ctcatgggcg cccgtcccta cggcatcgac cgtgaggtcg
      541 cccagcggat tgccgcggaa gcggccagtg cccacggcga gggtgtgcag gtcgcgatcg
      601 tggtcggcgg gggcaacatc ttccgcggcc tggccgcggc atcgaccggc atggaccgct
      661 ccacggccga ctacatgggc atgctggcca cggtcatgaa cggcctcgcc ctccaggacg
      721 cgctggagca ggcgggggct ccgacccggg tgatgagcgc gatcgcgatg aacgagatct
      781 gcgagccgta catccgccgg cgggcgatgc gccacctcga gaagggccgg gtcgtgatca
      841 tggtcgccgg gacagggaac ccgtacttca cgaccgacac cgctgccacc ctgcgcgcgg
      901 tcgagatcaa cgccgaggtc atcctgaagg ccacccgggt ggacggggtt tattccgccg
      961 atcccgagca ggatccgagc gccaagcggt acgcccagat cggctacacc gaggtcctct
     1021 ctaatcggct ccaggcgctc gatgccaccg cggtcagcct ggcgatggac aaccggatgc
     1081 cgatcgtcgt cttcgacatg accgtggatg gcaacattct ccgtgccctg cgaggtgagt
     1141 cgatcggaac cctgatcagc gacgaggtgc cggcatgagc catcaggcat tgcaggacgt
     1201 cgaacccaag atgcagcgcg cggtcgaggc catggagcgc gatttcgccg ggattcgcac
     1261 cggccgggcg tcgagcgcga tcgtcgagcg gatcacggtc gagtactacg ggaccgagac
     1321 cccgttgaac cagattgcgg gcatctcgac ccctgacgcg cacctgatcg tgatccagcc
     1381 gtgggaccgg agcgcgctcc ccgccatcga gaaggcgatc accaagagcg acatcggcct
     1441 gacgccgaac gtggatggca cggtcgtccg gctccacgtg ccgccgctgt cagaggaacg
     1501 ccggcatgag ctcgtgaagc aggtgcggcg ccggctggag gacgcgcggg tggagatccg
     1561 caacttccgc cgggaggccg cggacgccct caagcatgcg ctcaaggatg ggagccttgg
     1621 ccaggacgag gagcaccgcg agctcgaggc cctccagcgc ctgaccgatg gcttcgtcga
     1681 gatggccgag gcacgcggcg agcgcaaaga agcggagatc atggaggtct gaacgacctg
     1741 tccacaaccg agggcccgcg ccacgtcgcg atcatcatgg acggcaaccg ccgctgggcg
     1801 gcgagccgcg gtctcccggg catcgcgggc cacgtccagg g
//
LOCUS       DASYGU010000086        14931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_270617, whole genome shotgun sequence.
ACCESSION   DASYGU010000086 DASYGU010000000
VERSION     DASYGU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270617"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..69
                     /gene="infA"
                     /locus_tag="VGO32_02110"
     CDS             <1..69
                     /gene="infA"
                     /locus_tag="VGO32_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006688844.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV7199578.1"
                     /translation="DTVRVELSPYDLTRGRITYRMR"
     gene            66..179
                     /gene="rpmJ"
                     /locus_tag="VGO32_02115"
     CDS             66..179
                     /gene="rpmJ"
                     /locus_tag="VGO32_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV7199579.1"
                     /translation="MKVSASVKPRCERCKVIRRSGVVLVICRIPKHRQRQG"
     gene            189..560
                     /gene="rpsM"
                     /locus_tag="VGO32_02120"
     CDS             189..560
                     /gene="rpsM"
                     /locus_tag="VGO32_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV7199580.1"
                     /translation="MARIAGVDIPREKRVIISLTYIHGIGPATAAKVVTAASVSPDTR
                     VRDLTEDEVTRIREIVDKRYRVEGDVRREVSMNIKRLMEIGAYRGVRHRRNLPSRGQR
                     TKTNARQRRGSRRTVGVRRKK"
     gene            581..976
                     /gene="rpsK"
                     /locus_tag="VGO32_02125"
     CDS             581..976
                     /gene="rpsK"
                     /locus_tag="VGO32_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV7199581.1"
                     /translation="MAERKQKTRAKKREKKNVPAGHVHIQATFNNTIVTITDPGGGVI
                     AWGSAGLVGFKGSRKSTPYAAAQTAELAARKAMEHGVRQVEVFVKGPGAGREQAIRSL
                     QAAGLEVTAITDVTPIPHNGCRPPKRRRV"
     gene            980..1606
                     /gene="rpsD"
                     /locus_tag="VGO32_02130"
     CDS             980..1606
                     /gene="rpsD"
                     /locus_tag="VGO32_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV7199582.1"
                     /translation="MSRYTDPVCRICRREGLKLFLKGSRCYSKKCSFERRSTPPGMNP
                     TRRRKVSEYGLQMREKQKVRRSYSVVERQFRNYFRDAARQKGMTGENLLRMLEMRLDN
                     VVYRMGFATSRAQARQLVTHGHFNVNGRGTKSPGHQIAVGDRIEVRDTRRGRTYFQTA
                     SDTMRAAQVPDWVSADPSKLSGTVIAAPTREQMPMEFNEQLVVEYYSR"
     gene            1622..2641
                     /locus_tag="VGO32_02135"
     CDS             1622..2641
                     /locus_tag="VGO32_02135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV7199583.1"
                     /translation="MIEPESTATPRIEEIEAQGAAGRFEIQPLAAGYGVTLGNALRRV
                     LLSSLDGAAVTSIQVANVYHEFSTIPAVKEDVTQIVLNMKKIRLRSFAPHPVTLKLAK
                     HGAGLVTAGDIAESADVEIVNPDQELLTLDSDAGSIDMDITIETGVGYRAAEHTEEMP
                     IGVIPVDAIFSPVRRVNFSVENTRVGQMTNFDKLTLEIETDGTTTPQAALASAAGILV
                     REFSRFAEIGRPSVGTGDDVPAPLSASLLDAPIEELDLPMRAYNSLKRNNITKIGQLL
                     ALGDDELLRMRNFGKKSLDDMKERLALRGFIVPEDGTPAADEADLPAAAELDEAVAAE
                     LTREA"
     gene            2665..3006
                     /gene="rplQ"
                     /locus_tag="VGO32_02140"
     CDS             2665..3006
                     /gene="rplQ"
                     /locus_tag="VGO32_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV7199584.1"
                     /translation="MAGRKFSRPTAHREHMLANLAVSVLRYERLKTTEAKAKEVRGMV
                     DRLIVTAKKGDLPARRQLVAQLPNEPLIIEKLMGELAAKYSDRGSGFTRITRLGPRAG
                     DSAPMVQIELV"
     gene            3042..3827
                     /gene="truA"
                     /locus_tag="VGO32_02145"
     CDS             3042..3827
                     /gene="truA"
                     /locus_tag="VGO32_02145"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV7199585.1"
                     /translation="MPPEARRCARAVIEYDGTAFAGSQRQPRQRTVQGELEDALNKLT
                     GERITVRFAGRTDAGVHATGQVIAFCLPSRPRGERISWAELRRRLNAALTSDVVVRAL
                     RSAPPGFDPRREATMRTYRYRIREGGDRRPTDRHRTLEIPDHLDVAAMSDAAARLVGE
                     RDFAALGSDPRGQTDRHLMAVTVRRRGTLVEVTVTGNAFLRRMVRSLVAVLLAVGRGQ
                     MAPAEVERVLEARQRALHGRAVQACGLTLERISFERTGDVNRT"
     gene            3824..4270
                     /gene="rplM"
                     /locus_tag="VGO32_02150"
     CDS             3824..4270
                     /gene="rplM"
                     /locus_tag="VGO32_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV7199586.1"
                     /translation="MKTYAVKASEIDRGWWLIDATDQPLGRLATRVATLLSGKHKPTW
                     SPHLDTGDHVVVVNAARIRVTGNKLTQKRYYRHSNYPGGLREETLEDLLARKPERVIE
                     VAVKGMLPSNKLGNAMYRKLKVYPGAEHPHKAQRPQGAAEHEQEAS"
     gene            4267..4659
                     /gene="rpsI"
                     /locus_tag="VGO32_02155"
     CDS             4267..4659
                     /gene="rpsI"
                     /locus_tag="VGO32_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV7199587.1"
                     /translation="MSVNYLYGTGRRKTSVARVRLLPGDGSITVNGKPAEEYFGGETQ
                     VAPIRVPFTVTDTEGRYAASVLVAGGGTSGQAGAIRHGIARALLAGHPDFRPTLREAG
                     LLTRDPRAKERKKYGLKRARKAPQYTKR"
     gene            4748..5737
                     /gene="cdaA"
                     /locus_tag="VGO32_02160"
     CDS             4748..5737
                     /gene="cdaA"
                     /locus_tag="VGO32_02160"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEV7199588.1"
                     /translation="MPESISEFLNTFLRGSWLSIVDIVVVAILIYWLFVLIRGTRAVR
                     ILIGLSIVALVYLAAQALDLVLLSTLLQAGAVVGLFAVVVVFQPELRRGLEQIGRMGS
                     LNRLFVSSEVSAADTVAREVSRAARLLAGSRHGALMVIERETGLHDLTAESGIPINAD
                     LHAELLATIFYHGTALHDGAVVISGNRIVAAGVLLPLSQNVLDSERYGTRHRAAIGIS
                     EQTDALIVVVSEETGSISLVVRGRIERNLSEDRLRRRIFSLIRPQAPREAAPLLRRTL
                     EGRWGMPEEEPAEAPIDPEDRVVPSVAPPAKTPAETPDKAEATEPVASGERRA"
     gene            5737..6948
                     /locus_tag="VGO32_02165"
     CDS             5737..6948
                     /locus_tag="VGO32_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEV7199589.1"
                     /translation="MALLLGNWPLKLGALLLSILLYAGLVYSGSFTEQQMTGVPIRAT
                     NQPLNTYLLTGELGTVDVSYRASASVAGSVTISTFSASVDLAGYDMAQAGEPQLLAVE
                     VRSLDEDVTVLDAAPLEVSVILDRLAVRSVRVVVERGEIPPGLEVGSSEVMPATVQAQ
                     GPESRLREVTAALARVRIDSSGINVEEQVDLVPVDVDGQPVAAVELSPGTVTVQIEVS
                     TAETSRTVPVAPDLVGSPPTGLTLNGVSVEPAAVTLFGPPDVLAGVVSVATAPIDLAS
                     ITADGTIDVAIILPDETQLSAGAESSVQVTIDVDVEFASRTILAGPVCQNVGAGLTCE
                     PQVAQISVTVTGTRSAVAALTPAQVTPVLNMAGLTEGPHQVTPTVNLPAGITLVQPLG
                     SVAVNLTAGGP"
     gene            6967..8298
                     /gene="glmM"
                     /locus_tag="VGO32_02170"
     CDS             6967..8298
                     /gene="glmM"
                     /locus_tag="VGO32_02170"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV7199590.1"
                     /translation="MGRLFGTDGIRGVANVDLTPTLAYDLGRALGHYVDGAGRSVVVG
                     RDTRRSGEMLAAALAAGLTSVGTDVLDIGVVPTPCLAYVAEHGEHVAGVMVSASHNPP
                     DDNGLKVVSGGRKMDDEAEERLEHLIFQAEALPGPTNAGLGRVRHDPTAVEGYRAHLA
                     AIAGDSLAGLRIGVDAAHGSASVFGPDTLEALGAEVTAIHVDPDGTNINIGGGATEPT
                     ALADLVKARGLDMGFAFDGDADRLIAVSESGTIVDGDGVMGICALQRLAAGTLPESTL
                     VVSVMSNDGLVRAVEAAGGRVIRAPVGDRHIVEAMERSGAMLGGEQSGHIIFRDLAST
                     GDGLLTAIQLVAAIRAGGGASLGELAARIPRLPQVVINSGVRHKDQWQVDPLFADAVA
                     RATARLGSRGRILVRASGTEPKLRIMVEGEEPEEIATIARELADLATARLN"
     gene            8317..10152
                     /gene="glmS"
                     /locus_tag="VGO32_02175"
     CDS             8317..10152
                     /gene="glmS"
                     /locus_tag="VGO32_02175"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV7199591.1"
                     /translation="MCGIVGYVGPRDATPILLEGLRRLEYRGYDSAGIAVLTDAGTVF
                     VEKKAGKLSNLTDQLNGSSPHGHPGIAHTRWATHGAPTDLNAHPHADCTGKLALIHNG
                     IIENYVELRDRLAAAGHTFTSETDTEVLAHLIEERYEGDLVEAVRQALGQVQGAYAIG
                     VMHTDHPDLIVGARMNVPLIVGLGEGEGFLASDVPAILEHTRRIVILHEGDIAAVTPD
                     GTRVVGLDGDELERDVTEITWNLEAAEKGGFAHFTLKEIYEQPHAIQEAIRARVDGRG
                     GIHLPELAGIEDKLREADRIYLVACGTAHYASNVGAYLFQQWTGLPAVSVIGSEMRYA
                     PPPITDRTVVISVSQSGETADTIAPTRLARERGATVLVVTNVVGSALTRDGDATAYLQ
                     AGPEIGVASTKAFVTQVLVLQMIALHLAGLRGTMTSSQLATFGLGLRALPRAAVAALD
                     CATQVRDIARRWAEVNVQDVMYIGRGVGFPIAMEGALKLKELSYVHAEGYAAGELKHG
                     PIALLDADTPLVAIVTRGSVYDKVLSNVSEARARQAPVVAVATVGDEQIGAHAEDVLL
                     VPETLEELSPVISILPLQLLAYEVAVSRGTDVDQPRNLAKSVTVE"
     gene            10174..10554
                     /gene="acpS"
                     /locus_tag="VGO32_02180"
     CDS             10174..10554
                     /gene="acpS"
                     /locus_tag="VGO32_02180"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV7199592.1"
                     /translation="MSRHEIGVDLVDIDRIAQTLQRHPQRFRKRVLTEREDRYCGNKI
                     ERVAGRWAAKEAVSKVLGLGVRGVGWREIEVLPNFAGQPQVLLHDRAAARAARLGIGE
                     VTVSISHERRMAVAVAVAERLSES"
     gene            10561..11469
                     /locus_tag="VGO32_02185"
     CDS             10561..11469
                     /locus_tag="VGO32_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV7199593.1"
                     /translation="MAEAGTTLTQGWVAAHLPPRPAISHKGTFGRVLVVAGSLEYSGA
                     ALLTGLGAARGGAGLVCLATPESVGLRLLGLVPELTGLLLPEEAVGLVAPAGWRQLTA
                     AAAQYDAVVVGPGLGRHASTLRRAARLLGELRRPVVIDADGLTALAARERWWRTVTAP
                     AVLSPHPGEFARLLRDGNAPPDDDDAARETAANQAARLWEHVLVLKGANTVVASPDGQ
                     ILRSAVATPALATAGSGDVLAGVIGAMLAAGLSPLDAAGVGVAVHAAAGVLAEQRMGT
                     SGVLASDIAGLLPEATSALRRGAPDR"
     gene            11466..12599
                     /gene="alr"
                     /locus_tag="VGO32_02190"
     CDS             11466..12599
                     /gene="alr"
                     /locus_tag="VGO32_02190"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV7199594.1"
                     /translation="MTLIAMGAGHRAWLDIDHAALAANLATLRGRAGPDRQVIAVVKA
                     NAYGHGAIEVAQTLVEQGAERVAVATLSEAAELRASGQDAPLVLLWTVGADEAAEVVA
                     LGLEPVVDSTAYVDALATQAVAAGRRIGVHLKIDSGLGRQGIEPEHALELAARIVASP
                     GLVLTGTMTHLAAAADVAFTARQIDTFDSVLSNLRAGGIDAGIVHVGGTGAILADVGT
                     IATGIRPGIGLYGLRPGTADARAAGLTPVLSLRARPVRVFEVAAGTPIGYGMRWTAAR
                     RSRLATLPLGYGDGWPRAHLNNGWALVGGRRSPMVGAISMDGLVVDVTDAGPVTGDDE
                     FVLIGRQGDDEITADEVAEQRGTIGYEVTTMLRERLPRRHHRP"
     gene            12596..14257
                     /locus_tag="VGO32_02195"
     CDS             12596..14257
                     /locus_tag="VGO32_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199595.1"
                     /translation="MTTVRNPGATPAEPPGEARPRLAPTPVRVTPETELTLLLDVGSA
                     WTKAALVGRAGGRWRIVSGTAQPTAWGESVLVEDLVGRLTPHADARLARRLANLVREA
                     PRITCRSPRRAGRLAVVGDTTAGVESAMAVAVRSGWTVEAGVGGDDRKSDAERFTLLR
                     GVEVDAWLAVPSGGHGDRLWGLMAGARGPSSAPVVVVAADDQEDRFTALFGTGTRVIS
                     SVIDDRLAEALFDRLTAMNGLGQPRSATPVAFGRAMEALARGLGLTVLGIDAGSSWLA
                     WHHATGRDMGRTAVIAATPDQPLPSGSRAAAEVLPADVDEFTTTDAMASLAARRSAIP
                     ASPVEAAIAQAIGIDRLAAARRWLGGCPPVDLLVGGGRLLVGSSHPADAAMILLDGLR
                     PPGITQLALDPWGICGPLGGLPDADIDEALETLRDDLLMPLGTAVVSRGGQSGQLAFR
                     ARLHRPGWPDSTAVEVRAGSMTVLPLERGAAGELEIELERGVHIGPPVRGQRLRASVT
                     GGAVGIVLDARDDPIQLPTRPGDARSMIQAWRDALRRETRPGGTG"
     gene            14254..>14931
                     /locus_tag="VGO32_02200"
     CDS             14254..>14931
                     /locus_tag="VGO32_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199596.1"
                     /translation="MSGTDPYRHRIRLPAGAEPTVAVGDEVQPGDPVASSTRPGTSVS
                     VPLARPLHRGRDEVVDLLVVAPGGRVRVDQPIARTPDRDVRSPVDGIMLAYDPSKGDA
                     IVLGLEPSDPILSDVRGIVMEVNRGAVTIAVPAVMLAGVAGSGAAVHGPLTIAVTNAE
                     APLEPAMIDADAAGRIMVGGSWASAEVITRARAVGVAGIIVGGLHARELAEFTGLQHR
                     RSLLGTAA"
BASE COUNT         2288 a         5282 c         5218 g         2143 t
ORIGIN      
        1 gacaccgtcc gcgtggagct cagcccctac gacctcaccc ggggtcgaat cacgtacagg
       61 atgcgatgaa ggtttctgcc tcggtcaagc cccgctgcga gcgctgcaag gtgattcggc
      121 gctcgggtgt ggtgctggtc atctgccgaa tccccaagca ccgccagcgg cagggctagg
      181 agcaggtcgt ggcgcgcatt gctggcgttg acattccccg cgagaagcgg gtgatcatct
      241 cgctcaccta catccacggc atcggcccgg ccaccgcggc caaggtcgtg accgcggcca
      301 gcgtgtcacc tgacacccgc gtccgggacc tgaccgagga cgaggtcacg cgcatccgcg
      361 agatcgtaga caaacggtac cgggtcgaag gcgacgtccg ccgcgaagtg agcatgaaca
      421 tcaagcgact gatggagatc ggcgcctacc gcggcgttcg ccaccggcgc aacctcccat
      481 cgcgcggcca gcggacgaag accaacgccc gtcagcgccg cggctcgcgc cgaaccgtcg
      541 gcgtgcggcg caagaagtag ccccgtagca agcgagaccc atggctgagc gaaagcaaaa
      601 aacgcgcgcg aagaaacgcg agaagaagaa cgtcccggcc gggcatgttc acatccaggc
      661 cacgttcaac aacacgatcg tgaccatcac cgatccgggt ggtggcgtga tcgcctgggg
      721 aagcgccggg ctggtcgggt tcaagggctc gcgcaagagc accccgtacg ccgcggcaca
      781 gaccgccgag ctcgccgccc gcaaggcgat ggagcacggc gtccgccagg tggaggtctt
      841 cgtgaagggg ccgggcgccg gccgcgagca ggccatccgc tccctccagg ccgccggcct
      901 cgaggtcacc gcgatcaccg acgtgacgcc catcccgcac aacggctgcc gcccacccaa
      961 gcggcggcgg gtctaggcca tgtctcgtta caccgatccg gtctgccgga tctgccgccg
     1021 cgagggcctc aagctcttcc tgaagggcag ccgctgctac tccaagaagt gctcgttcga
     1081 gcggcggagc accccgcccg ggatgaaccc cacccggcgt cggaaggtca gcgagtacgg
     1141 gctccagatg cgcgagaagc agaaggtccg tcgcagctac tcggtcgtcg agcgccagtt
     1201 ccggaactac ttccgtgacg cggcgcgcca gaagggcatg accggcgaga acctgctccg
     1261 gatgctcgag atgcgcctcg ataacgtcgt gtaccgcatg ggcttcgcca cctcccgagc
     1321 ccaggctcgc cagctggtga cccacggcca tttcaacgtg aacgggcgcg ggaccaagtc
     1381 ccccggccac cagatcgcgg tcggcgaccg gatcgaggtg cgggacacgc gccgcggtcg
     1441 gacgtatttc cagacggcgt ccgacacgat gcgcgccgcc caggtcccgg actgggtgag
     1501 cgccgatccg tccaagctgt ccggaaccgt cattgccgca ccaacccgtg agcagatgcc
     1561 catggagttc aacgagcagc tcgtcgtcga gtactactcg cgctaggagt tccccttgct
     1621 gatgatcgaa cccgagtcca ccgccactcc gcgtatcgaa gagatcgagg cgcaaggcgc
     1681 cgccggtcgc ttcgagatcc agcccctggc cgccggctac ggcgtcacgc tgggtaacgc
     1741 cctgcgccgc gtcctgctct cgtcacttga tggagcagcc gtgacctcga tccaggtggc
     1801 gaacgtgtac cacgagttca gcacgatccc ggccgtcaag gaagacgtga cccagatcgt
     1861 cctgaacatg aagaagatcc gactgcggtc cttcgccccc cacccggtga ccctcaagct
     1921 cgccaagcac ggggccgggc tggtgacggc cggcgacatt gccgagtccg cggacgtcga
     1981 gatcgtgaac cccgaccagg agctgctcac gctcgactcc gacgcggggt cgatcgacat
     2041 ggacatcacg atcgagaccg gcgtgggcta ccgcgccgcc gagcacaccg aggagatgcc
     2101 gatcggggtc atcccggtgg atgccatctt ctccccggtt cgccgggtga acttctcggt
     2161 cgagaacacc cgggtcggcc agatgacgaa cttcgacaag ctgaccctcg agatcgagac
     2221 cgatgggacg acgacgccgc aggcggcgct ggcctccgcg gccgggatcc tggtccgcga
     2281 gttctcgcgc ttcgccgaga tcggccgccc gtcggtgggc accggcgacg acgtcccggc
     2341 tccgctgtcg gccagcctgc tggacgcccc gatcgaggag ctcgacctgc cgatgcgggc
     2401 ctacaacagc ctcaagcgca acaacatcac gaagatcggc cagctcctgg cgctcggcga
     2461 cgacgagctg ctccggatgc ggaacttcgg caagaagagc ctcgacgaca tgaaggagcg
     2521 cctggccctc cgcggcttca tcgttcccga agatggcacg ccggcggccg acgaggccga
     2581 cctgccggcc gcggccgagc tcgacgaggc cgttgccgcc gagctgaccc gcgaggcgta
     2641 ggaaacacct cgatgccgca tcgcatcgcc ggacgcaagt tcagcaggcc cacggcccat
     2701 cgcgagcaca tgctcgcgaa tctcgccgtg tccgtcctgc gctacgagcg cctgaagacc
     2761 accgaggcca aggccaagga ggtgcgcggc atggtggatc ggctcatcgt gaccgccaag
     2821 aagggcgatc taccggcccg acggcagctg gtggcccagc tgcccaatga accgctcatc
     2881 atcgaaaagc tgatgggtga actggctgcc aagtattccg atcgggggtc ggggttcacg
     2941 cgcatcacgc gcctgggacc gcgggccgga gactcggcgc cgatggtgca gatcgagctc
     3001 gtctaaggcg ggctcgatcg gccgattaca accgaagacc gatgccaccc gaggcgcgcc
     3061 gttgcgcccg ggcggtgatc gagtacgacg ggacggcgtt cgccggctcg cagcgccaac
     3121 cgcgacagcg gacggtgcag ggtgagctgg aggacgcgct taacaaactg accggcgaac
     3181 ggatcacggt ccgattcgcc gggcgcaccg atgccggcgt gcatgccacg ggccaggtga
     3241 tcgccttctg cctgccgagc cgcccccgtg gggagcggat ctcgtgggcg gagctgcggc
     3301 gacgcctcaa cgcggcgctc acgtcggatg tggtggtccg cgcgctgcgg tccgcgcctc
     3361 cggggttcga cccgagacgc gaggccacaa tgcggacgta ccgctatcgg atccgggagg
     3421 gcggcgaccg ccgtccaacc gaccggcatc ggacgctcga gatccccgat cacctggatg
     3481 tggccgccat gagcgatgcc gcagcccggc tggtggggga gcgcgacttc gcggccctcg
     3541 gcagcgaccc gcgtggccag accgatcgcc acctgatggc ggtgacggtc cggcgacgag
     3601 ggaccctcgt cgaggtgacg gtcacgggga acgcgttcct tcgccggatg gtgcgaagcc
     3661 tggtggcggt gctgctcgcg gtcggtcggg gccagatggc ccccgccgag gtggagcggg
     3721 tgctcgaggc ccggcaacga gccctccatg ggcgcgcggt ccaggcctgt gggctcaccc
     3781 tcgagcggat cagtttcgaa cgaacaggag acgtgaaccg aacgtgaaga cttacgcggt
     3841 caaggccagc gagatcgacc gaggctggtg gctgattgac gccaccgatc agccgctcgg
     3901 ccggttggcc acgcgtgtcg ccacgctgct ctccggcaag cacaagccaa cctggtcacc
     3961 gcatctggac accggcgacc acgtcgtggt cgtgaacgcg gcgcggatcc gggtgaccgg
     4021 caacaagctc acccagaagc gctactaccg tcactcgaac taccccggtg gcctgcggga
     4081 ggagaccctc gaggatctcc tcgcccggaa gccggagcgc gtgatcgagg tcgcggtcaa
     4141 ggggatgctg cccagcaaca agctcggcaa cgcgatgtac cgcaaactga aggtctaccc
     4201 cggagccgaa cacccgcaca aggcgcagcg cccgcaggga gccgccgagc atgagcagga
     4261 ggcgtcgtga gcgtcaacta cctgtacgga accggccgcc gcaagacgtc ggtggcccgc
     4321 gtccgtctcc tgccgggcga cggcagcatc acggtcaacg gcaagcccgc cgaggagtac
     4381 ttcggcggcg agacccaggt ggcgccgatc cgcgtcccgt tcacggtgac cgacaccgag
     4441 ggccgctacg cggccagcgt gctggtcgcc ggtgggggca cgtcgggcca ggccggagcc
     4501 atccgccatg gaatcgcccg tgcgctgctt gccggccatc cggatttccg acccacgctc
     4561 cgcgaggccg ggctcctgac ccgcgacccg agggccaagg agcggaagaa gtacggcctc
     4621 aagcgcgctc gcaaggcgcc gcagtacacg aagcgctgat catgccggct cccggaccga
     4681 tccgggggcg cccgagaagg cctttggtaa catcctcggc agaccgcagg ggagctcctg
     4741 tctgatcgtg ccggagagca tcagcgaatt cctgaataca ttcctgcgcg gttcgtggct
     4801 ctcgatcgtc gacatcgtcg tcgtcgccat cctgatctac tggctgttcg tgctcattcg
     4861 gggcacccgc gcggtccgca tcctgatcgg cctttcgatc gtggcccttg tctacctggc
     4921 ggcgcaggcc ctcgatctcg tgctcctctc caccctcctc caggcgggcg ccgtggtcgg
     4981 cttgttcgcg gtcgtggtcg tgttccagcc cgagctgcgc cgcggtctgg agcagatcgg
     5041 ccggatgggc tcgctcaatc ggctgttcgt gtcatcggag gtgtcggccg cggacacggt
     5101 tgcgcgcgag gtctcacgag cggcgcgcct gctggccggg tcgcgccacg gtgcgctgat
     5161 ggtgatcgag cgggagacgg gactgcacga cctgaccgcc gagtcgggca ttccgatcaa
     5221 cgccgacctc cacgccgagc tgcttgccac catcttctat cacggcaccg cgctccatga
     5281 tggcgcggtc gtcatctccg gcaacaggat cgtggcggcg ggcgttctgc tcccgctcag
     5341 ccagaacgtg ctcgacagcg agcgctacgg cacccgtcat cgggccgcga tcgggatcag
     5401 cgagcagacc gatgcgctca tcgtggtggt cagcgaggag accggctcca tctcgctcgt
     5461 ggtacgcggt cggatcgagc gcaacctgag cgaggatcgg ctccgccgtc ggatcttcag
     5521 cctcatcagg ccacaggcgc cgcgcgaagc ggcaccgctc ctccggcgca cgctcgaggg
     5581 gcgctgggga atgccagagg aagaaccggc cgaggcaccc atcgaccccg aggaccgggt
     5641 cgtcccatcc gtcgcgccgc cggccaagac gcccgccgag acgccggata aggccgaggc
     5701 gaccgagccg gtggccagcg gagaacgccg cgcctgatgg ccctgctgct cggcaactgg
     5761 cccctcaagt tgggtgccct gctcctctcg atcctgctgt acgcaggcct cgtgtactcc
     5821 ggctcgttca cggagcagca gatgaccggg gtcccgatcc gtgccacgaa ccagccgttg
     5881 aacacgtacc tgctcaccgg ggagctcggg accgtcgatg tcagctatcg ggcctcggcc
     5941 agtgtcgccg ggagcgtgac gatctcgacc ttcagcgcct ccgtcgacct cgccgggtac
     6001 gacatggcgc aggccggcga gccgcagctc ctcgcggtcg aggttcgctc cctcgacgag
     6061 gacgtgacgg tcctcgacgc cgcgccgctc gaggtgtcgg tcatcctcga ccggttggcg
     6121 gtccgatcgg tccgcgtcgt cgtagaacgt ggagaaattc cccccggact ggaggtgggc
     6181 agctccgagg tgatgcccgc cacggtccag gcgcagggtc cggagagccg gcttcgcgag
     6241 gtcaccgcgg ctctggcccg agtccgcatc gactcgtccg gcatcaacgt cgaagagcag
     6301 gtcgacctcg tgccggtgga cgtggacggg cagccggtcg ccgccgtcga gctgtccccg
     6361 ggcacggtca ccgtgcagat cgaggtgagc accgccgaga cctcgaggac ggtgccggtc
     6421 gctcctgacc tggtcggcag tccgccgacc ggcctcaccc tcaacggggt cagcgtcgag
     6481 cccgccgccg tgaccctgtt cggtcccccg gacgtcctgg ccggggtcgt gagcgtggcc
     6541 accgccccga tcgacctggc cagcatcacg gccgacggca cgatcgacgt cgccatcatc
     6601 ctgcccgacg agacgcagct gtcggccgga gccgagtcct cggtccaggt cacgatcgac
     6661 gttgacgtcg aattcgcaag ccgcacgatt ctcgccggcc ccgtatgtca gaacgtgggt
     6721 gcgggcctca cctgtgagcc ccaggtggcc cagatctcgg tcacggtcac gggcacgcgc
     6781 tcggcggtcg cggctctgac ccccgcccag gtcacccccg tcttgaacat ggccggcctt
     6841 accgagggac cccaccaggt gaccccgacc gtgaacctcc ccgccggcat caccctcgtc
     6901 cagcccctcg gatcggtggc ggtgaacctc actgccggcg gtccctgacc gcgaccggca
     6961 gctgaggtgg gccgcctatt cggcactgac ggcatccgcg gagtcgccaa tgtcgacctg
     7021 accccgaccc tggcctatga cctcggccgc gcgctgggcc actacgtgga cggcgccggc
     7081 cggagcgtgg tggtcgggcg cgatacgcgc cgctcgggcg aaatgctcgc cgcggccctg
     7141 gctgccgggc tgacctcggt cggcaccgac gtgcttgata tcggcgtggt gcccacgccg
     7201 tgcctggcct acgtggcgga gcacggcgag cacgtggcag gggtgatggt ttccgcctcc
     7261 cacaaccctc cggacgacaa cgggctgaaa gtcgtgtccg gggggcggaa gatggatgac
     7321 gaggccgagg agcggctgga gcacctcatc tttcaggccg aggccctgcc cgggcccacg
     7381 aacgcgggcc taggtcgtgt tcgtcatgac ccgaccgcgg tcgaaggcta tcgggcacac
     7441 ctggcggcga tcgccggcga tagtctcgcc ggcctgcgga tcggggtcga cgccgcccat
     7501 gggtccgcat cggtgttcgg gcccgacacg ctcgaggccc tcggggcgga ggtgaccgcc
     7561 atccacgtcg atcccgacgg cacgaacatc aacatcggtg gaggcgcgac ggagcccacc
     7621 gcgctcgccg acctggtgaa ggcccggggc ctggacatgg gcttcgcatt tgacggcgat
     7681 gccgaccggc tgatcgcggt gagcgagtcc ggcacgatcg tggacggcga cggcgtgatg
     7741 ggcatctgcg ccctccaacg gctggctgcg ggaacgctgc ccgagtcgac cctggtcgta
     7801 tcagtcatga gcaatgacgg gctggtgcga gccgtggagg cggccggtgg ccgggtcatc
     7861 cgggccccgg tgggggaccg gcacatcgtg gaagcgatgg agcgctcggg agccatgctc
     7921 ggaggcgagc agtcggggca catcatcttt cgcgatctgg cctcgaccgg cgacgggctg
     7981 ctgaccgcca tccagctcgt cgccgcgatc cgcgccggag gtggggcgag tctcggcgag
     8041 ctggccgcca gaattccgag gcttccgcag gtggtgataa actccggcgt ccgccacaag
     8101 gaccagtggc aggtcgaccc gctttttgcc gatgccgtgg cgcgggcgac agcccggctg
     8161 ggcagccggg gccggattct cgtccgcgcc tcgggtactg agcccaagct gcggatcatg
     8221 gtcgaaggtg aggagcccga agagatcgcc accattgccc gagaactcgc cgacctggcg
     8281 accgcacggc tcaactagca gggcccggag ggaaccatgt gcgggatcgt gggatatgtc
     8341 ggaccgcgtg acgcgacgcc gatcctgctc gagggcctgc ggcgcctcga atatcgcggc
     8401 tacgactcgg ccggaatcgc ggttctgact gatgccggaa ccgtgttcgt ggagaagaag
     8461 gcgggcaagc tctcgaacct caccgaccag ctgaacgggt ccagcccgca cggccatccg
     8521 ggtatcgcgc acacccgctg ggcgacccac ggtgccccga ccgatctgaa cgcccacccc
     8581 catgctgact gcaccggcaa gctggcgctc atccacaacg ggatcatcga gaactacgtc
     8641 gagctccggg accggctggc tgccgccggg cacacgttca cctcggagac cgataccgag
     8701 gtcctcgccc acctgatcga agagcggtac gagggcgacc tggtcgaggc ggtccgccag
     8761 gccctgggcc aggtccaggg cgcctacgcg atcggggtca tgcacaccga ccaccccgac
     8821 ctcatcgtcg gcgcccgaat gaacgtgccg ctcatcgtcg ggctcggcga gggagaaggc
     8881 tttctggcca gtgacgtgcc cgccatcctg gagcacacgc gccggatcgt catcctccac
     8941 gaaggggaca tcgcggccgt gacgcccgac ggcacgcggg tcgtgggcct cgatggtgat
     9001 gagctcgaac gagacgtgac cgagatcacg tggaacctgg aagcggccga gaagggcggg
     9061 ttcgcccact tcacgctgaa ggagatctac gagcagccgc atgccatcca ggaggccatt
     9121 cgcgcccggg tcgacggacg cggcggcatc catctccccg agctggcggg catcgaggac
     9181 aagcttcgcg aggcggaccg gatctacctc gtggcctgcg gtacggccca ctacgcttcg
     9241 aacgtagggg cctacctgtt ccagcaatgg acagggctgc ccgcggtctc ggtcatcggc
     9301 tcggagatgc gctacgcccc gccaccgatc accgatcgca cggtcgtcat cagcgtcagc
     9361 cagtcgggcg agaccgcgga cacgattgcg cccacccgac tggcgcgtga gcgtggcgcc
     9421 acggtccttg tcgtgaccaa cgtggtgggc agcgcgctga cccgcgacgg cgacgcgacg
     9481 gcgtacctcc aggccggccc cgaaatcggg gtggcctcga ccaaggcctt cgtgacccag
     9541 gtcctcgtcc tccagatgat cgccctccac ctggccggtc tgcgcgggac gatgacgtcc
     9601 tcgcagctgg ccaccttcgg gctcgggctc cgtgcgctgc cccgagcggc cgtggccgcg
     9661 ctcgactgcg cgacgcaggt tcgcgacatc gcccgacgct gggccgaggt caacgtccag
     9721 gacgtcatgt acatcggtcg cggagtcggc ttcccgatcg cgatggaggg cgcgctcaag
     9781 ctcaaagagc tgtcgtatgt gcacgcggag ggctatgccg cgggcgagct caagcacgga
     9841 ccgatcgcgc tgctcgacgc cgacacgccg ctggtggcga tcgtcacccg cgggtcggtc
     9901 tacgacaagg tcctgtcaaa cgtgtccgag gcccgggccc gccaggcacc ggtggtcgcg
     9961 gtcgccacgg tcggcgacga gcagatcgga gcccatgcgg aggacgtcct cctcgtcccg
    10021 gagacgctcg aagagctgtc cccggtcatc agcattctcc cgctgcagct gctcgcctac
    10081 gaggttgccg tttcccgagg cacggacgtc gaccagccgc gcaacctcgc caaatcggtc
    10141 accgtcgagt agacgacggg tgggcgccgc cccatgagcc gccacgagat cggagttgac
    10201 ctggtcgaca tcgatcggat cgcccaaacc ctccagcgcc acccgcagcg cttccgcaag
    10261 cgggtcctga ccgagcgcga ggaccgatat tgcggcaaca agatcgaacg ggtcgccggc
    10321 cgctgggcgg cgaaggaggc ggtgagcaag gtcctcggac tgggcgtgcg cggtgtcggc
    10381 tggcgcgaga tcgaggtgct gcccaacttc gccggccagc cgcaggtcct gctccacgat
    10441 cgcgcggcgg ctcgcgccgc gcgccttggt atcggcgagg tcaccgtgtc gatctcccac
    10501 gagcggcgga tggcggtcgc ggtcgcggtc gccgagcgtc tgtccgaaag ctgattgccg
    10561 atggcggagg cgggcaccac gctgacccag ggctgggttg cggcccacct gccgcctcga
    10621 cccgcgatca gccacaaggg aaccttcggc cgcgtcctcg tcgtcgccgg atcgctcgaa
    10681 tattccggcg cggcgctcct gaccggcctg ggtgcggcac gcggcggggc agggctcgtc
    10741 tgcctggcga cacccgaatc cgtcggtctg cgcctgctgg gcctcgtccc ggagctgacc
    10801 ggcctgctcc tgcccgagga ggccgtgggc ctcgtggccc cagccggctg gcgccagctg
    10861 accgccgccg ccgcccaata cgacgccgtg gtggtcggtc cggggctggg acgccatgcc
    10921 tcgaccctcc ggcgcgccgc ccgtctgctc ggcgagctcc ggcgcccggt ggtgatcgat
    10981 gccgatggcc tgaccgcgct ggctgcccgc gagcgctggt ggcgtaccgt gactgcgccg
    11041 gccgtcctga gcccgcaccc gggcgagttc gcgcgccttc tgcgcgatgg aaacgcgcca
    11101 cccgacgatg acgacgccgc tcgcgaaacc gcggccaacc aggccgcccg gctgtgggag
    11161 cacgtcctcg tcctgaaggg cgccaacacg gttgtggcct caccggacgg acagatcctg
    11221 cgctccgcgg tggccacgcc cgccctggct acggccggca gcggcgacgt cctggctggg
    11281 gtcatcggcg ccatgctggc cgccggcctg tcgcccctcg acgcggcggg ggtcggcgtg
    11341 gcggttcatg cggctgccgg ggtcctcgcc gagcagcgaa tggggacctc cggcgtcctt
    11401 gcctcggaca tcgccgggct tctaccggag gccacgtccg ccctccgacg gggggccccc
    11461 gaccggtgac cctcatcgcg atgggcgccg ggcaccgggc gtggctggac atcgaccacg
    11521 cggccctggc cgccaacctg gccacgctcc gtggccgggc gggcccggat cgacaggtca
    11581 tcgcggtcgt gaaggccaac gcctacggcc atggggcgat cgaggtcgcg cagactctcg
    11641 tcgagcaggg ggcagagcgc gtggcggtgg cgaccctgtc cgaggccgcc gagctgcggg
    11701 ccagcggcca ggacgctccg ctcgtcctgc tctggaccgt cggcgcggac gaagcggccg
    11761 aggtggtcgc cctcggcctg gaaccggtgg tcgacagcac cgcgtacgtg gacgccctgg
    11821 ccacccaggc ggtcgcggcc ggacgtcgaa tcggcgtgca tctgaagatc gacagcgggc
    11881 tcggccggca ggggatcgaa cccgagcacg cgctcgagct ggcggcccgc atcgtggcca
    11941 gcccgggtct ggtcctcacc gggacgatga cccatctcgc ggccgccgcc gacgtggcgt
    12001 tcacggcgcg gcagatcgac acgttcgatt cggtcctctc aaacctccgc gcgggcggaa
    12061 ttgacgcagg aatcgtgcac gtcggcggga cgggcgccat ccttgccgac gtgggcacca
    12121 tcgccacagg gatccggccg ggaatcgggc tctacgggct ccgcccgggc acggcggacg
    12181 ctcgcgccgc cgggctgacg cccgtgttga gcctccgcgc ccggccggtc cgcgtcttcg
    12241 aagtcgcggc cgggaccccg atcgggtacg gcatgcgctg gaccgcggcg cgccgatcac
    12301 ggctcgccac gctgcctctc ggctacggcg atgggtggcc gcgagcccat ctgaacaacg
    12361 gctgggcgtt ggttggtggc cggcggagcc cgatggtcgg cgcgatcagc atggacggac
    12421 tggtggtgga cgtgaccgat gccgggccgg tcaccggcga cgacgagttc gtgctcatcg
    12481 gacgccaggg ggacgacgaa atcaccgccg acgaggtggc cgagcagcgc ggcacgatcg
    12541 gatacgaggt gacgacgatg ctccgtgagc gcctcccccg gcggcaccac cggccatgac
    12601 caccgtgcgc aaccccgggg cgacgccggc cgagcccccg ggcgaggcgc ggccgcgcct
    12661 ggcgccgaca ccggtccgag tgacacccga gaccgagctc accctcctgc tggacgtggg
    12721 cagcgcatgg acgaaggcgg ccctcgtcgg gcgagccggc ggtcgatggc ggatcgtgag
    12781 cgggacggcc cagcccacgg cgtggggtga atcggtcctg gtcgaggacc ttgtcggccg
    12841 gctgacgccg catgccgatg cgcgcctcgc gcgccgcctg gcgaacctcg tccgcgaggc
    12901 cccgcggatc acctgccgat cgccacgccg cgctggtcgg ctggccgtgg tcggggacac
    12961 gaccgccggc gtcgagagcg ccatggcggt cgccgtccgg agcgggtgga cggtcgaggc
    13021 aggcgtcgga ggcgatgacc gcaaatcgga cgccgagcgg ttcaccctcc tgcgtggtgt
    13081 cgaggtggat gcctggctcg cggtgccgtc cggggggcac ggcgatcggt tgtggggcct
    13141 gatggccggt gcgcgaggtc catcctccgc cccggtggtg gtcgtggctg ccgacgacca
    13201 ggaggatcgg ttcacggccc tgttcgggac cgggacccgc gtaatctcgt cggtcatcga
    13261 cgaccgcctc gccgaagccc tgttcgatcg gctgaccgcg atgaatgggc tcggccagcc
    13321 ccggtcggcc actccggtcg ccttcggacg ggcgatggaa gcccttgctc ggggtctcgg
    13381 cctgaccgtc ctcggtatcg acgccgggtc ctcctggctg gcatggcacc acgccaccgg
    13441 tcgcgacatg ggccgaactg ccgtgatcgc cgccacgccc gaccagccgc tgccaagtgg
    13501 ctcgcgagcc gcggcggagg ttctcccggc cgacgtggac gagttcacga ccaccgatgc
    13561 gatggcaagc ctcgccgccc ggcgatccgc gatcccggcc tccccggtcg aggccgccat
    13621 cgcacaggcg atcggcatcg accggctcgc cgccgctcgg cggtggctcg gcggctgccc
    13681 cccggtcgat ctgctggtcg gtggcggccg gctcctggtc ggctcgtcgc acccggccga
    13741 tgccgccatg atcctgctcg atgggctccg gccgccgggc atcacccagc ttgcgctcga
    13801 tccgtggggg atctgcgggc cgctgggcgg actgccggac gcggacatcg acgaggcgct
    13861 cgagaccctc cgtgatgacc tgctcatgcc gctcggtacg gcggtcgtgt cgcgcggtgg
    13921 gcagtcggga cagctcgcct tccgtgcgcg actccaccgt cccggctggc ccgactccac
    13981 cgcggtcgag gtccgggcag gatcgatgac cgtcctgccc ctcgagcgcg gcgcggcagg
    14041 cgagctcgag atcgagctcg agcggggcgt tcatattggt ccccccgtcc ggggccagcg
    14101 cctgcgggcg tcggtgaccg gcggggccgt gggcatcgtg ctcgatgcgc gcgacgaccc
    14161 gatccagctt cccacgcgcc ccggcgatgc ccgttccatg atccaggcct ggcgcgacgc
    14221 gctccgtcgc gagacgcggc cggggggcac aggatgagcg ggaccgatcc gtatcggcat
    14281 cggatccgcc ttccagcagg cgccgagccc accgttgcgg tcggtgacga ggtccagccc
    14341 ggcgacccgg tggcctcctc gacgcgaccg gggaccagcg tctcggtgcc gctggcccgc
    14401 ccccttcatc gcggccggga cgaggtcgtt gatctcctgg tcgttgcgcc cggcggccgg
    14461 gtgcgggtgg accagcccat cgcccgcacc cccgaccgcg acgttcgctc accggtggac
    14521 gggatcatgc ttgcctacga cccgtcgaag ggcgacgcca tcgtgcttgg cctggagcca
    14581 tccgatccga tcctgagtga tgtccgaggg atcgtgatgg aggtcaaccg cggcgccgtc
    14641 acgatcgccg ttccggccgt catgctggcc ggggtggcgg gcagcggggc ggcggttcac
    14701 ggtccactca cgatcgcggt caccaacgcc gaagcgcccc ttgagccggc catgatcgac
    14761 gccgatgccg cgggccggat catggtcggt gggtcgtggg cctcagccga ggtcatcact
    14821 cgggcccggg cggtcggcgt ggccggcatc atcgtcggcg gcctccacgc ccgcgagctg
    14881 gccgagttca ccggcctcca gcatcgccgc agcctgctcg gcaccgccgc c
//
LOCUS       DASYGU010000087         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1505353, whole genome shotgun sequence.
ACCESSION   DASYGU010000087 DASYGU010000000
VERSION     DASYGU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505353"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGO32_02205"
     CDS             complement(<1..233)
                     /locus_tag="VGO32_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV7199597.1"
                     /translation="MVAHRGASQAAPENTMEAFRLGVEAGADAIEVDVHLTSDGELAV
                     IHDATLDRTTDRTTTVATATMAAIREADAGWAFP"
     gene            complement(239..1585)
                     /locus_tag="VGO32_02210"
     CDS             complement(239..1585)
                     /locus_tag="VGO32_02210"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199598.1"
                     /translation="MSAARDVTALAPARWWRGLGGLTRSGSSVEGLSWALYDFANTIF
                     SFAIVSFAMGPWTTRALGEANGTLAFTVAGSLSVLLNAIVSPALGAVSDRTGRRKRYL
                     LVFTVLCIVPTAVIGFVNIWLGLLAFAIANFAFQAALIYYDAILPDVAKPEKRGRLSG
                     IGVGLGYMGTIVSGLLLRYTTDDEGLITAASFVLVASLFAIFAIPIFLVVKERPITGA
                     IPFRLADALRSWRQLGVTWQHAGEQPGLRRFIVGRFFYSDGVNTAIAVMSLFTINAIG
                     FTEGEALYVLLGLTVVAVIASFGWGYLADRWGPKRTLLIVLGSWVLGLLIMGLVTTKP
                     AFLVAGAILGSGLGGTAVTDRLLLLRLAPPERVGEMFGLFGLAGKFSAVIGPVVFGVI
                     IFLLVDSLGTVAYQVAILSLLGLMVLGYWIVRGVPEPPPEAGDAAQAALAGSPPSP"
     gene            1669..3987
                     /locus_tag="VGO32_02215"
     CDS             1669..3987
                     /locus_tag="VGO32_02215"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7199599.1"
                     /translation="MFAALGRFDYRFRRWIPPIGLVLVIGLSVWASQAGGELSQGGWQ
                     IEGSESSATAELLADRFGTQATSLFVILRDPAGDAASEAFADVVDHAVAPLADDPAVD
                     DITTYADTGDPRLLSTDGTTTFAVVRLNSPEEEALDDTRRLVELVEQPAGVDEMWVTG
                     VPVVTIEYNEIIESDLQKAEVISFVAAGIILLVVFGTVVGAGLPLVIAGLSLLATFAA
                     ISLLANQLDMSIFVTNLATMIGLALAIDYSLFLVSRFREELHHHPVDIAVERTMATVG
                     KAVFVSGVAVAVGLAALIVFEADALRSMGIGGVVVVVATLLFGMTVLPALLAMLGPRV
                     NRFRVPLPKAIRSVEVDPDEADRRRGHGFWSRVAARVMRRPVLIGGPILVLLLLAGTP
                     FFRIQLSTGGNLEDLPDTPAKEGFIVLRDEFPGGDTDPMIAAVTYDGVADLAAGELTP
                     DRLADLRAYVDEVSVLPDIDGVQSVLDPPPGLPAEQYLAALAAPVEAWPAGLSDWVAQ
                     TVADDTTKVTIFSTRLPDTDAGRELVAEVRDVADPSGSTVGVTGLAARSADFMDSFRS
                     SVPVAVAIVIAVTLIVLFLTFGSVFLPIKAVLMSLISITASFGALVWIFQEGHLSGLL
                     NFEATGSLGAWLPILMFAILFGLSMDYEVLLLSRIRERWVATGNNTQAVAEGIGITGG
                     TITGAAMIMVVVFSAFAISDVLFLKAIGFSMALGVLIDATAVRGVLVPAFMRVMGRWN
                     WWAPAWVQRAVARLGLYEGPAPPPASQEKAPA"
     gene            4040..5215
                     /locus_tag="VGO32_02220"
     CDS             4040..5215
                     /locus_tag="VGO32_02220"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7199600.1"
                     /translation="MTLPLEGIIVLDCSRVLAGPHATMLLADLGADVWKLEPPGGDET
                     RAWGPPFWGDPADRRSAYFAAVNRDKRSLVVDLKTEAGHDVFDRLASRADLLVHNYLP
                     SAATRLGMDPARLRERHPHLVVSVVRGFPGDGPLADRPAYDLVAQAWSGQMAVTGEPG
                     GGPIKFGVGLLDLLAGLEAAIAALAALAARERGAAPAQVSVSLVEAAVAGLANVLGNH
                     LATGAEPRRWGTGHPDIVPYQVFAARDGHVVVAVGNDPQFARLCRLLGVEPRPEWATN
                     PERVVQRDEVLAALTGPIGTWARDDLVTALVAADIPGGPVHAVGEAIAAMEDIETDWV
                     STVDGVRLPASPIRVDGERLPISRPPPRLGEHTDEILRTVGVAPDDLKVLRRQNVIA"
BASE COUNT          780 a         1830 c         1799 g          826 t
ORIGIN      
        1 ggaaaggccc agcccgcatc ggcctcgcgg atggcggcca tcgtcgcggt cgcgaccgtc
       61 gtcgttcgat cggtcgtccg gtccagggtg gcgtcgtgga tgaccgccag ctccccgtcg
      121 gaggtgaggt ggacgtcgac ctcgatcgca tcggcgccgg cctcgacccc gagccggaag
      181 gcctccatcg tgttctccgg ggccgcctgc gacgcccccc ggtgggcgac caccagggtc
      241 acgggctcgg tgggcttccc gcgagcgctg cctgggcggc atcgccggcc tcgggcggtg
      301 gctcggggac gccgcgcacg atccagtagc ccaggaccat gaggcccagg agcgagagga
      361 tcgctacctg gtaggcgacc gtgcccagcg aatcgaccag caggaagatg atgaccccga
      421 agaccaccgg gccgatcacc gcgctgaact ttccggccag accgaacagt ccgaacatct
      481 cacccaccct ctcgggcggc gcgaggcgga ggaggagcag tcgatcggta accgcggtgc
      541 cgccgaggcc cgatccaagg atcgcgccgg ccaccaggaa tgcaggtttc gtggtcacga
      601 ggcccatgat gagcagtccg aggacccatg accccaggac gataaggagg gtccgctttg
      661 ggccccagcg atccgcgagg tagccccagc cgaatgaggc gatcaccgcg accacggtca
      721 ggccgaggag cacgtagagc gcctcgccct cggtgaagcc gatcgcgttg atcgtgaaca
      781 ggctcatcac cgcgatggcc gtgttcacgc cgtcggagta gaagaaccga ccgacgatga
      841 agcggcggag acccggctgc tccccggcgt gctgccacgt gacacccagc tgccgccacg
      901 agcggagtgc gtcggccaac cggaacggga tggcaccggt gatcggcctc tccttgacca
      961 cgaggaagat ggggatcgcg aagatggcaa acagcgacgc gaccaggacg aagctggcgg
     1021 cggtgatgag cccctcgtcg tcggtcgtgt agcgcagcag cagccccgac acgatcgtcc
     1081 ccatatagcc caggcccaca ccgatccccg acaggcgccc ccgcttctcg ggcttggcga
     1141 cgtccggcag gatcgcgtcg tagtagatga gcgcggcctg gaacgcgaag ttcgcgatcg
     1201 cgaatgcgag cagcccgagc cagatgttga cgaagccgat gaccgcggtg gggacgatgc
     1261 acaggaccgt gaacacgagc aggtagcgct ttcgacgccc ggttcggtca ctcacggcgc
     1321 ccagcgcggg cgagacgatc gcgttgagca ggaccgacag actcccggcc acggtgaagg
     1381 ccagcgtccc gttcgcctcg cccagcgcgc gggtggtcca cggccccatc gcaaagctca
     1441 cgatcgcgaa cgagaagatc gtgttcgcga agtcgtacag ggcccacgac aggccctcga
     1501 ccgagctgcc cgaacgggtc agaccaccca gcccgcgcca ccagcgggct ggagccaggg
     1561 ccgtcacgtc gcgcgccgcc gacacatgcg gcgccgatca tagcccccac ccgaacgctc
     1621 ccttggcgct tccggcggga aacgagatgg tgggctagag tgacggccgt gttcgccgcc
     1681 ctcggccgct tcgactaccg attccggcga tggatcccac ccatcggcct ggtgctggtc
     1741 atcggtcttt cggtctgggc cagccaggcc ggaggcgagc tttcgcaggg cgggtggcag
     1801 atcgagggct cggagtcgtc ggccaccgcc gagctcctgg ccgaccgatt cggcacgcag
     1861 gcgacctcgc tcttcgtgat cctgcgtgac cccgccgggg acgcggcttc ggaggccttc
     1921 gcggacgtgg tcgaccatgc ggtcgcgccc ctggccgatg acccggcagt cgacgacatc
     1981 acgacctacg ccgacaccgg cgatccgcgc ctgctctcga ctgacggcac cacgaccttc
     2041 gcggtggtcc gcctgaactc gcccgaagag gaagctctag acgacacgcg ccgcctggtc
     2101 gagctcgtcg agcaaccagc cggcgtcgac gagatgtggg tcaccggcgt gccggtggtg
     2161 acgatcgagt acaacgagat catcgagtcg gacctccaga aggcggaggt catcagcttc
     2221 gtcgccgccg gcatcatcct gctcgtcgtc ttcgggacgg tggtgggcgc cggcctccca
     2281 ctcgtcatcg ccggcttgtc gctgctggcc accttcgccg cgatcagcct gctggcgaac
     2341 cagctcgaca tgtcgatctt cgtgaccaac ctggccacca tgatcggtct ggcgctggcc
     2401 atcgactact cgctcttcct cgtcagccgc ttccgggaag agctccacca ccacccggtc
     2461 gacatcgcgg tcgagcgcac gatggcgacc gtcggcaagg ccgtgttcgt ctccggcgtc
     2521 gcggtggccg ttggcctcgc cgccctcatc gtgttcgagg ccgacgccct gcgatcgatg
     2581 ggcatcggtg gggtggtggt ggtcgtggcc accctgctgt tcggcatgac cgtcctgccc
     2641 gccctcctcg cgatgctcgg cccacgcgtg aatcggttcc gtgtcccgct gccgaaggcg
     2701 atccgctcgg tggaggtgga ccccgacgag gccgatcgcc ggcgcggcca tggcttctgg
     2761 tcgcgcgttg cggctcgggt gatgcgtcgg ccggtgctca tcggtgggcc gatcctcgtc
     2821 ctccttctcc tggccggaac ccccttcttc cggatccagc tgtcgaccgg cggcaacctc
     2881 gaggacctgc ccgatactcc ggccaaagaa gggttcatcg tcctccgcga cgagttcccc
     2941 ggtggtgata ccgatccgat gatcgccgcc gtgacctacg acggcgtcgc cgacctggcc
     3001 gccggcgagc tgacgcccga ccgcctggcc gatctccggg cctacgtcga cgaggtgagc
     3061 gtactgccgg acatcgatgg ggtccaatcg gtcctcgatc cgccgccggg cctgcccgca
     3121 gagcagtacc tcgccgccct ggcggccccg gttgaggcct ggcccgcggg cttgtccgac
     3181 tgggtggccc agacggtggc ggacgatacg accaaggtca cgatcttctc gactcgcctg
     3241 cccgacaccg acgccggccg cgagctcgtg gccgaagtcc gcgacgtcgc cgatccatcc
     3301 gggtccaccg tgggcgtcac cgggctggct gcgcgctcgg ccgacttcat ggacagcttc
     3361 cgatccagcg tgccggttgc cgtcgcgatc gtgatcgcgg tcactctgat cgtcctgttc
     3421 ttgacgttcg gatcggtgtt cctgccgatc aaggccgtgc tcatgagtct catctcgatc
     3481 acggccagct ttggcgcgtt ggtctggatc ttccaggaag ggcacctctc gggtctgctg
     3541 aacttcgagg ccacggggtc gctcggcgca tggctcccga ttctcatgtt cgccatcctg
     3601 ttcgggctgt cgatggacta cgaggtcctg ctcctgtccc gcatccggga gcgctgggtg
     3661 gccaccggca acaacaccca ggcggtggcc gaggggatcg ggatcacggg cgggaccatt
     3721 actggggcgg cgatgatcat ggtcgtcgtg ttctcggcgt ttgccatctc ggacgtcctg
     3781 ttcctcaagg ccatcggctt ctcgatggcc ctcggcgtgc tcatcgatgc gaccgccgtg
     3841 cgcggcgtcc tggtcccggc cttcatgcgg gtcatgggcc gctggaactg gtgggcaccc
     3901 gcctgggtcc agcgggccgt cgcacggctc gggttgtacg aaggtcccgc gccaccgcct
     3961 gcgagccagg agaaggcccc cgcgtgactc gcgtcggcgc tagccttggc gcgccgatgt
     4021 cggatgttca gctgaccgcg tgaccctgcc tctcgagggg atcatcgtcc tcgactgctc
     4081 gcgcgtgctc gccggaccac acgcgacgat gctgcttgcc gatctgggcg cggatgtctg
     4141 gaagctggag ccgcccggtg gcgacgaaac gcgtgcatgg ggaccgccat tctggggcga
     4201 cccggcggac cggaggagcg cctacttcgc cgcggtcaac cgcgacaagc gcagcctcgt
     4261 agtcgacctc aagaccgagg cggggcatga cgtctttgat cggcttgcgt cgcgcgcgga
     4321 cctgctggtc cacaactatc tcccctcggc ggccacccgg ctggggatgg atcccgcgcg
     4381 actgagggag cgtcaccctc acctcgtggt ctcggtggtg cgcggcttcc cgggggacgg
     4441 cccgctcgcc gaccggcccg cctacgacct cgtggcccag gcgtggagcg gccagatggc
     4501 agttaccggc gagcccggcg gcggaccgat caagttcggg gtcggcttgc tggacctgct
     4561 tgccggcctg gaggccgcga tcgcggccct tgccgcgctg gccgcccggg agcgaggagc
     4621 cgcgccggcg caggtgtcgg tgagcctggt cgaggcagcc gtggcgggcc tggccaacgt
     4681 gctgggcaac cacctcgcga ccggcgccga gccgcgccgg tgggggaccg gccacccgga
     4741 catcgtcccg taccaggtct tcgcggcccg ggatggccac gtggtcgtgg ccgtgggcaa
     4801 cgacccccag ttcgcccggc tgtgccgctt gctcggcgtg gaaccgcgtc cggagtgggc
     4861 caccaacccg gagcgggtcg tgcagcgcga cgaggtactc gcggcgctga caggcccgat
     4921 cgggacgtgg gcccgcgacg atctggtcac ggccctggtc gccgccgata tcccgggcgg
     4981 gccggtccac gccgttggcg aggccatcgc cgcgatggag gatatcgaaa ccgactgggt
     5041 ctcgaccgta gacggagttc gcctgccggc ttcacccatc cgcgtcgacg gggagcggct
     5101 gccgatcagt cgaccaccac cgcggctggg cgagcacacg gacgagatcc tgcgaacggt
     5161 tggcgttgcg ccggatgacc tgaaagtact acgccgccag aacgtgatcg cctagcgcac
     5221 gcatcggctg gcggg
//
LOCUS       DASYGU010000088        14229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_580151, whole genome shotgun sequence.
ACCESSION   DASYGU010000088 DASYGU010000000
VERSION     DASYGU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580151"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VGO32_02225"
     CDS             complement(<1..340)
                     /locus_tag="VGO32_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199601.1"
                     /translation="MLVAVVLLGLVVACSFTPAGSSVTAAAALDLVRGQDPLFTGVMA
                     HNPEAIGQVGWFEQTETEDGWEIVIRIGWEDCPAGCISEHRWTYTVTAAGEVTLVEES
                     GDPLPTDTGVR"
     gene            complement(396..1316)
                     /locus_tag="VGO32_02230"
     CDS             complement(396..1316)
                     /locus_tag="VGO32_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV7199602.1"
                     /translation="MRRFLLWCARNEWLRVHIPRLPGAQRAVRRFMPGEDMDDALKAA
                     GALQAKGIPSIFTHLGENLTDLSQADEVAGHYHELITRFARWGLRPDVSPKLTQLGLD
                     LDPDATFEHALGVARHTREAGGRFWIDMEDSSYVDRTLDIYERLLAAQPGIGVCIQAY
                     LRRTPEDIERLRPLRASIRLVKGAYNEPAERAFRSKTEVRKAYRDLSIEMLADASAGR
                     MTLGLATHDVELIEEIAAVASGSGIGRDAFEVQMLYGIRADQQRRLRDEGYRVRVLIA
                     YGEYWYPWYVRRLAERPANLWFAVRQMIPW"
     gene            complement(1372..1764)
                     /locus_tag="VGO32_02235"
     CDS             complement(1372..1764)
                     /locus_tag="VGO32_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV7199603.1"
                     /translation="MSRSWRTGALLTVGLATLAVACSPGASERPAASAPAGSTTVNVV
                     LTNAGCTPDVDSVPAGTIMFEATNDGGNAVDEVELLAGEDVLAEAENLAPGLSGSFTV
                     DLEPGSYLLLCPGAEVPETPFEVVEASS"
     gene            complement(1761..2618)
                     /gene="efeU"
                     /locus_tag="VGO32_02240"
     CDS             complement(1761..2618)
                     /gene="efeU"
                     /locus_tag="VGO32_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEV7199604.1"
                     /translation="MLATFVIGLREGLEAALIVGIVAAFLRQAGRADALRFVWIGVAL
                     TVAACIALAFGLQLWSTNLPYKEQEALETVLGFAAVGMVTYMILWMRSHARGLRGNLE
                     AATASALATGSTVALVTMAVFAVLREGLETAVFLLAAFNASESALAAGLGALLGVVVA
                     AAVGYGIYRGGVRLNLARFFRITGVVLVFVAAGLVASSLHSAHEAGWIVIGQTAVADL
                     RWLVQPGALLSALFTGVLGIPAEPTVIEAIGYFAYLVPMLIVVLRPARPPQPTSPTMV
                     EAGSGAATR"
     gene            complement(2687..3400)
                     /locus_tag="VGO32_02245"
     CDS             complement(2687..3400)
                     /locus_tag="VGO32_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV7199605.1"
                     /translation="MASTAASQEYLVTLRNLISSAQPATVTALARRLHVSPQAASEMI
                     HRLQGEGLVDHGDGRQLTLTRRGRAQSDATFRRHALMEWLLTGVIGMGWAESDQEAHR
                     LQASISDALGKQLDEFLGHPETCPHGNPIDAATARRRPPGQPLSSFASGAEATIYRIT
                     EEAEEDEALLSYLEARALQPGAPVVVRASSGVVDAITVDGPRGSATLGLRPASLILAL
                     PGAADPALFHRLPTLRSAD"
     gene            3510..4862
                     /gene="xylA"
                     /locus_tag="VGO32_02250"
     CDS             3510..4862
                     /gene="xylA"
                     /locus_tag="VGO32_02250"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEV7199606.1"
                     /translation="MPSDFFTEVTAPIPFAGLDSAEPLSFKVYEADRLVIGRRMEDHL
                     RIAVCLWHSFNWPGSDVFGAGTFDRPWLTGDAEPMAAARDKVAAAFEFLTKLGVPYFC
                     FHDRDVAPEGRTFSETQGNLEQMIAEIEGHMARTGKKLLWGTANLFSHPRYAAGASTN
                     PDPEVFAYAAAQVKLMLEATHRLGGANYVLWGGREGYETLLNTDLAREEDQFARFLTL
                     VAEHKHRIGFTGTLLIEPKPQEPTKHQYDYDCATVAGFLQRHGLTDEYRVNIEVNHAT
                     LAGHSFHHEVLVALATGIFGSVDINRGDYQNGWDTDQFPNSVDELAPVLAEILQAGGF
                     TTGGFNFDTKLRRQSLDRSDLFHAHIGGMDTLARSLLVAADMVEQRTLDRFRTDRYAG
                     WTAELGESILGGRMSLADLAAAVDERKLDPQPRSGRQELLENLVNQRIWAAERSPRTE
                     "
     gene            4882..6366
                     /gene="xylB"
                     /locus_tag="VGO32_02255"
     CDS             4882..6366
                     /gene="xylB"
                     /locus_tag="VGO32_02255"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEV7199607.1"
                     /translation="MGHVLGIDVSTTATKAILVDETGAVRGVGTAEYPTSVPQPRWSE
                     QDPTQWWEGSVSAIQSILASSRVPAEEVAAVGLTGQMHGAVLLDAADAVLRPAILWND
                     QRTNAECDLIREAVGRDRLIEITGNDALTGFTAPKLVWVRDHEPDVWRRVAHVLLPKD
                     YVRLRLTGDRAVDKADGAGTILFDLAARDWSPEVLEALEINRAWMPTTYEGPEVTGKL
                     TADAARATGLRAGTPVVAGGGDQAANAVGVGVVAEGTMALSLGTSGVVFAATSEPRIE
                     PEGRVHAFCHAIPGRWHMMSVMLSAAGSLRWFRDALAPGLSFEELTAGAAEVPAGSDG
                     LTFLPYLSGERSPHPDPFARGAFVGLTLAHDRRHMVRAVLEGVAYGLRDGLDLMVEAG
                     TLAPDQIRASGGGTASGLWRQILADVLEAEIATVETQEGAAYGAGLLAAVGARWHPNV
                     ESATAALVKATPVAAPGLARARYIEGHAAYRSLYPALRPLFPRA"
     gene            complement(6389..7213)
                     /locus_tag="VGO32_02260"
     CDS             complement(6389..7213)
                     /locus_tag="VGO32_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199608.1"
                     /translation="MTVEILPPSTPPSPAAPRAPLVDMRDIRVSFGGVHAVDGVNIDL
                     HEGEVVGLVGGNGAGKSTLMKVLSGAQSADAGEIHIRGEVASISSPRDAKTYGIECIY
                     QTLALADNLDCPANIFLGRELVSRVGSLDDDAMEAASRTVLNRLNPNFKNFAAPVVSL
                     SGGQRQAVAIGRAIHFEAKILIMDEPTAALGPAETAQVRDVIKQLKEEGIGIFLISHD
                     IHDVFDLADRISVMYHGRIVDTVEKGSVTTDEVLAMIILGKKAAEASREELAEIQI"
     gene            complement(7225..8562)
                     /locus_tag="VGO32_02265"
     CDS             complement(7225..8562)
                     /locus_tag="VGO32_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007205343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7199609.1"
                     /translation="MTGAEAQLQPPAYQRVLGAIREFLRRIPVALRRAEIDTRLFGML
                     VALAIIWVGFNVVSDGGFLTARNLWNLSVQSASIGIMATGMVLVIVSRNIDLSVGSLL
                     GFLGYTMAMVQIEWIPRALGLGFDQPYTWLVALVVGLVLGATLGAITGLIIGYGGVPS
                     FIVTLGGLLVFRGLIFSYAQGQTMAPLDGIFRLLGGGPEGSLGETLSWAVGVVIAVAI
                     VINAVRSRRRKRHYGFQVRPMWAEIALNGFAIAAVLGAVWVANAYSWPEALAAQFAEE
                     RGIPIPPGGLIIPTGIAYPVLILLAVTIVMTYVATRRRFGRNVYAMGGNPEAARLGGI
                     NTRKTIVTIFAVMGVLCAISAAVQTARLNAAVTGLGGQNELDVISAAVIGGTSFAGGI
                     GSIPGAVLGAVVMQSLRSGMLLLNVDSPTQDIIVGFVLVIAVGLDAYLRRRAG"
     gene            complement(8664..9764)
                     /locus_tag="VGO32_02270"
     CDS             complement(8664..9764)
                     /locus_tag="VGO32_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012178670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7199610.1"
                     /translation="MSFRRVAAILSVFAILLVACQPSSSGSPAESAAGSAGESAAAGG
                     DCVVGVSWNNYQEERWAKWDEPAIQEALGAGNAEYVSTDAGSSEEQQANDVENLLSQG
                     ANVLIILAQSGEAILPSVQTAIDQGVPVIAYDRLIEDPATLYITFDNVGVGRLQAEVI
                     FGLVPEGNYAIIKGNSADANADFLRAGYEEVIGDAVTSGAITIVGETYTDNWDPATAQ
                     TNMEQILTDANNEVDAVLSENDGMAGGVVAALEAQGMAGTTPVSGQDGDLAALNRVAL
                     GTQSVSVWKDARDLGTAAGVAALALCGDPDITAVPNTAQFDSPGGNSMTSILLTPLAI
                     TQDNLNAVVDAGWIDEETLCQGVTAGSVDACP"
     gene            10032..10739
                     /locus_tag="VGO32_02275"
     CDS             10032..10739
                     /locus_tag="VGO32_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7199611.1"
                     /translation="MDRLNGKVALVTGASRGIGAAVAQSLADAGARLALGSRSGDDPG
                     IEGALTMPADVRDPASVEALVAATVDQFGGLDILVVNAGVGAYGPFLDLPAEHIEEMI
                     DVNVKGAIYAIRAALPVLLKSDAADIVMIASEAGRRGLPYEAVYCASKFAQVGLTAAL
                     DHELREQGVRCTNVCPGGVATDFAMGRGRTPDMPQLSGMMRPEDVAETVLFVVTRPRT
                     HRILEVAFRPVTETSWG"
     gene            10745..11731
                     /locus_tag="VGO32_02280"
     CDS             10745..11731
                     /locus_tag="VGO32_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7199612.1"
                     /translation="MTNLRWGILSTADIGRTKVIPGIQKGNRCDVVAIASRDEGRARE
                     TARALGIPRAHGSYEALLADAEVDAVYIPLPNHLHAEWTLAAARAGKHVLCEKPLATT
                     AADAERMIEVCRAEGVQLMEAFMYRLHPSWVAVRELVAGGRIGQLVAVDSWFSYFNDD
                     PANIRNIAAVGGGALYDIGCYSVNLSRMLFGSEPTGIHAVLRRDPVGGTDVLTSGLLE
                     FGDGVATFTCSTRAEDDQRVEIYGTTGRISIGIPFNIPPDRPTEVFVTAGGNPPVDPD
                     TEVLTFATADPYTVEAEYFAAAVLEDRPVPVPPEDAVANLHVIEQILAVARS"
     gene            11756..13450
                     /locus_tag="VGO32_02285"
     CDS             11756..13450
                     /locus_tag="VGO32_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEV7199613.1"
                     /translation="MSDDPSGRPVRVLLGVAIAALLGLAVVALLGRATFGSQPEALPA
                     PRFIDDTDRAGFDHRYDGEFAFFVGGGVAVFDCDGDRKPDIYFAGGSEPAALYRNESE
                     VGGPLRFVPVVSPVTDLDDVTGAYPLEVDGDGVIDLAVLRRGGNVLLRGIGACAFEAA
                     NDAWSFDGGTAWTAAFSATWETTTGWPTLAFGNYLDQSSENPERLCADNVLVRPKPDG
                     AGFAAPVPLSPGWCSLSILFSDWDRSGRRDLRVSNDRHYYSATGDGEEQLWRISRAEP
                     PHLYTEAEGWQRVQIWGMGIASYDLTGDGYPEYFLTSQADNKLQTLEDGPTEPRFRDM
                     ALEAGVTATRPYVGDTTLPSTAWHAEFQDVNNDSFVDLFVGKGNVEAMPEYAAADPSN
                     LLIGQTDGTFVEGAETAGIVSYDRARGAALADFNLDGMLDLVVVYRREPVRVWRNAGP
                     AYSVGTAQAPASHWLAVQLGQEGTNTQAIGAWVEVRIGDRVIQREMTVGGGHASGQLG
                     WTHFGVGPATEVEVRVQWPDGEVGPWLPVDADQFVLVERDADAAEPWVPAAGPTQP"
     gene            13471..>14229
                     /locus_tag="VGO32_02290"
     CDS             13471..>14229
                     /locus_tag="VGO32_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEV7199614.1"
                     /translation="MAELSLPEFGSSPSTPGIPTELYEQRVGRLRARADEAGFDRLVV
                     YADREHSANLAYLTGFDPRFEEAVLVVGPDDPPAILVGNECYAMAGAAPLAMRRHLLQ
                     DLSLPSQPRDRSRPLADLLAEEGIGPGSRVGVIGWKTYGDAEASDAPAYLVDEIRRLT
                     GPAGSVTNATGLLIDAGDGLRVMNEVEQLAAFEHAACHTSSGVRRLLFGLQPGMTEQD
                     AVGLLAWNGSPLSCHLMLSSGPRATLGLLSPGDRT"
BASE COUNT         2274 a         4862 c         4960 g         2133 t
ORIGIN      
        1 cccggacccc ggtgtcggtg gggagcgggt caccggactc ctcgacgagg gtgacctcac
       61 ccgccgcggt gacggtgtag gtccaccggt gctcgctgat gcatcccgcc gggcagtcct
      121 cccagccgat gcggatgacg atctcccagc cgtcctcggt ctcggtctgt tcgaaccagc
      181 cgacctggcc gatggcctcg ggattgtgcg ccatgacccc cgtgaagagc gggtcctggc
      241 cgcgcacgag gtcaagcgcc gcggcagcgg tgaccgaaga cccggccggg gtaaacgagc
      301 aggcgacgac aagcccgagc aacacgacgg cgaccaacaa ccggatgagc gtcatgttcg
      361 ctggacgccc gcaggcgtgg tgaggttccg gaccgtcacc agggaatcat ctggcgcacg
      421 gcgaaccaca ggttcgccgg ccgctcggcc aggcggcgga cgtaccacgg ataccagtac
      481 tcgccgtagg cgatgagtac ccgcacccgg tagccctcgt ctcggaggcg acgctgctgg
      541 tcggctcgga tgccgtacag catctggacc tcgaacgcgt ctcgcccgat ccccgaccca
      601 gaggcaacgg ccgcgatctc ctcgatcagc tccacgtcgt gggtggccag gcccagggtc
      661 atccgcccgg ccgaggcatc ggccagcatc tcgatcgaca ggtcacgata ggccttcctg
      721 acctccgtct tggaccggaa ggcccgctcg gcgggctcgt tgtacgcgcc cttgaccagc
      781 cggatcgatg cccggagcgg ccgaagccgc tcgatgtcct cgggagtccg gcgcagatag
      841 gcctggatgc agaccccgat ccctggctgg gccgcgagca ggcgctcata gatgtccagc
      901 gtccgatcca cgtaggagga gtcctccatg tcgatccaga accgcccgcc cgcctcgcgg
      961 gtgtgccggg caacccccaa cgcgtgctcg aaggtcgcat cgggatcgag atccaggccc
     1021 agctgggtca gcttgggcga tacgtcgggg cggagccccc atcgggcgaa tcgggtgatg
     1081 agctcgtgat agtggcccgc gacctcgtcg gcttggctga gatcggtcag gttttcgccc
     1141 aggtgggtga aaatcgacgg aatgcccttc gcctgcaggg cgccggccgc tttgagcgca
     1201 tcgtccatgt cctcgccggg catgaaccgc cgcacggcgc gctgggcgcc gggcagccgg
     1261 ggaatatgga cgcgaagcca ctcgtttcgg gcgcaccaca gcaggaatcg gcgcacgcgt
     1321 cagcctgtgc tacgggaagc ggcccagtgt acgggaggcc gctcccgccg atcaggacga
     1381 ggcttcgacg acctcgaacg gcgtttcggg cacctcggcg cccgggcaga gcaggaggta
     1441 gctgcccggc tccagatcga ccgtgaagct cccggagagg ccgggcgcca ggttctcggc
     1501 ctcggcgagc acgtcctcgc ccgccagcag ctcaacctcg tcgaccgcgt tgccgccgtc
     1561 gttggtggcc tcgaacatga tcgtgccggc cggcacggag tcgacgtccg gcgtgcagcc
     1621 ggcgttggtc agcaccacgt tgaccgtggt cgaccccgcc ggcgcgctag ccgcgggacg
     1681 ttccgacgca ccgggcgagc acgccaccgc gagggtcgcc agaccaacgg tcagcaaagc
     1741 gccggtgcgc cacgaacggc tcatcgggtt gcggctcctg atcctgcttc gaccatggtg
     1801 ggcgaggtcg gctgcggtgg tcgagcgggc cgcaacacga cgatgagcat ggggacgagg
     1861 taggcgaagt agccgatggc ctcgatcacg gtcggctcgg ccgggatgcc gagtacgccg
     1921 gtgaacaggg ccgacagcag ggcgccgggc tggaccagcc agcggaggtc cgcgaccgcg
     1981 gtctggccga tgacgatcca tccggcctcg tgggccgagt ggagcgacga ggcgaccagg
     2041 ccggccgcca cgaagacgag gaccacgccc gtgatccgga agaaacgggc gaggttgagt
     2101 cggacgcccc cgcggtagat gccatacccg accgcggccg cgaccacgac cccaagcagg
     2161 gctcccaggc cggcggcgag ggcgctctca ctggcgttga acgcggcgag caggaagacg
     2221 gcggtttcca gaccctcgcg aaggaccgcg aagacggcca tcgtcacgag cgccaccgtg
     2281 ctgccggtgg ccagggccga cgccgtggcc gcttcgaggt tgccccgcag gccacgggcg
     2341 tgcgagcgca tccacaggat catgtaggtc accatgccca ccgcggcgaa gccgaggacg
     2401 gtttcgaggg cctcctgctc cttgtacggc aggttcgtcg accacagctg gagcccgaag
     2461 gcgagggcga tgcaggcggc cacggtcagg gcgacaccga tccacacgaa gcgcagtgcg
     2521 tcggctcggc cggcctggcg caggaacgcg gcaacgatgc cgacgatcag cgcggcctcg
     2581 agtccctcac ggagaccgat gacgaaggtg gcgagcacgg cgcgagccta gacggtcccc
     2641 cgctccggtg tcaaggagcc ttgtgattct tcttcagggt cccctgtcaa tcggcgctcc
     2701 ggagcgtcgg caggcggtgg aagagggcgg gatccgccgc tccgggcagg gccaggatca
     2761 atgacgccgg acgcaggccc agggtggcgg agccgcgcgg tccgtcgacc gtgatcgcgt
     2821 ccaccacgcc actgctggcc cggacgacga ccggggcccc gggctggagg gcacgggcct
     2881 ccaggtacga gaggagcgcc tcgtcctcct ccgcctcctc ggtgatccgg tagatcgtcg
     2941 cctccgcacc ggaggcgaac gagctgagcg gttggccggg aggccgtcgc cgggccgtcg
     3001 cggcgtcgat cgggttgccg tgggggcatg tctccgggtg gccgaggaat tcgtcgagct
     3061 gcttcccgag cgcatccgag atggacgcct gcagccgatg cgcctcctga tctgactccg
     3121 cccagcccat accgatcacg ccggtcagga gccactccat cagagcgtga cggcggaacg
     3181 tggcgtcgga ctgagcccga ccgcggcggg tgagggtcag ctggcgcccg tcgccatggt
     3241 caaccaggcc ctcgccctgg aggcggtgga tcatctcgga ggcggcctgc ggagagacgt
     3301 ggagccggcg ggcgagggcg gtgacggtgg ccggctgcgc ggagctgatc aggttgcgca
     3361 gggtgacgag gtactcctgc gaagcggccg tcgatgccat ggctcactca ggatagtcgg
     3421 gccacccctc cgaccggggg tcatcgggcc ccatcggcgg ggctttcctt gggctccggc
     3481 cggccgtcgc cgtgtgctac ggtcgaagca tgccgagtga tttcttcacg gaggtgacgg
     3541 caccgatccc attcgccgga ctggactccg ccgaaccgct ctcgttcaag gtctacgagg
     3601 ccgatcgact cgtcatcggc aggcggatgg aggaccacct tcggatcgcg gtctgcctgt
     3661 ggcactcgtt caactggccc ggctcggacg tgttcggtgc cgggaccttc gaccggccat
     3721 ggctgacggg tgacgcggaa ccgatggcgg ccgcgcgcga caaggtcgcg gccgcgttcg
     3781 agttcctgac caagctcggc gtcccctact tctgcttcca cgaccgcgac gtcgcgccgg
     3841 agggtcggac cttctccgaa acgcagggca acctggagca gatgatcgcc gagatcgagg
     3901 gccatatggc ccggaccggg aagaagctgc tgtggggcac ggcgaacctg tttagccacc
     3961 cacggtacgc ggctggcgca tcgacgaacc ccgatccgga ggtcttcgca tatgccgccg
     4021 cccaggtcaa gctgatgctc gaggccacgc atcggctcgg tggcgcgaac tacgtgctgt
     4081 ggggggggcg ggagggttac gagaccctcc tgaacaccga cctggcccgc gaagaggacc
     4141 agttcgctcg gttcctgacc ctggtcgccg agcacaagca tcggatcggc ttcaccggaa
     4201 cgctccttat cgagcccaag ccgcaggagc caacgaagca ccagtacgac tacgactgtg
     4261 ccaccgtggc cggattcctc cagcgacacg ggttgaccga cgagtaccgg gtgaacatcg
     4321 aggtcaacca cgcgaccctg gccgggcaca gtttccacca cgaggtcctc gtggcgctgg
     4381 cgaccgggat cttcggcagc gtggacatca accgcggcga ctaccagaac ggatgggaca
     4441 ccgatcagtt cccgaactcg gtcgacgagc tggcgccggt cctggccgag atcctgcagg
     4501 caggcggctt cacgaccggt ggcttcaact tcgacaccaa gctccgccgc cagagcctgg
     4561 atcggtccga cctgttccat gcccacatcg gcggtatgga caccctcgca cggtcgctcc
     4621 tcgtggcggc cgacatggtg gagcagcgga cgctcgatcg gttccggacg gatcggtacg
     4681 ccggctggac cgctgagctg ggcgagtcga tcctcggcgg ccgaatgagc ctcgccgacc
     4741 tcgccgccgc cgtggacgag cggaagctgg acccccaacc ccgatctggg cgccaggagc
     4801 tgctcgagaa cctcgtcaac cagcgcatct gggccgcgga acgctcgccg cggaccgagt
     4861 gaccgcgcga cactgagcgc gatgggtcac gtcctcggga tcgacgtctc gacgacggcg
     4921 accaaagcca tcctggtcga cgagaccggc gccgtccgcg gggtgggcac ggcggagtac
     4981 ccgacgtcag tccctcagcc acgctggagc gagcaggatc cgacccaatg gtgggaagga
     5041 tcggtgtccg cgatccaatc gattctggcc tcgagtcgcg tcccagccga ggaggtggcc
     5101 gccgtcggcc tcaccgggca gatgcacggg gccgtcctgc tggacgcggc tgacgccgtc
     5161 ctgcgcccgg ccatcctgtg gaacgaccaa cggaccaacg ctgagtgcga cctgatccgc
     5221 gaggccgtcg gccgcgatcg gctcatcgag atcaccggca acgacgcgct gaccgggttc
     5281 accgcgccca agcttgtctg ggtccgtgac cacgaacccg acgtgtggcg ccgtgttgcc
     5341 cacgtgctcc tgcccaagga ctacgtccgc ctgcggctga ccggcgaccg cgccgtggac
     5401 aaggccgatg gggcgggcac gatcctcttc gacctcgctg ctcgcgactg gtcgcccgag
     5461 gtgctcgagg cgctggagat caaccgcgcc tggatgccaa cgacgtacga aggccccgag
     5521 gtcaccggga agcttaccgc tgacgcagcc cgggccaccg gtctgcgcgc gggcaccccg
     5581 gttgtcgctg gtggcggcga ccaggcggcc aacgcggtcg gagtcggcgt cgtggcagag
     5641 ggcacgatgg cgctctcgct cggaacatcg ggggtcgtat tcgccgctac ctccgagccg
     5701 cggatcgagc ccgagggtcg tgtccatgcc ttctgccacg ccatccccgg ccgctggcac
     5761 atgatgtcgg tgatgctctc agcagcgggc agcctgcggt ggttccgcga cgcgctggcg
     5821 ccgggcctgt cgttcgagga gctcaccgcc ggagcggctg aggtgcccgc aggaagcgac
     5881 gggcttacct tcctccccta tctgtcaggt gaacggagcc cgcacccaga tcccttcgcc
     5941 cgcggcgcgt tcgtcggtct caccctggct catgaccgcc gtcacatggt gcgtgccgtc
     6001 ctcgagggcg tcgcctacgg cctgcgcgac ggcctggacc tgatggtcga ggccggaacg
     6061 ctggcgcccg atcagatccg ggcttcaggt ggggggacgg ccagcggcct ctggcggcag
     6121 atcttggccg acgtccttga ggcggaaatc gcaacggtcg agacccagga gggagcggcc
     6181 tacggcgcgg gcctcttggc cgcggtcggc gcacgttggc atccgaatgt cgagtcggca
     6241 accgccgccc tcgtcaaggc cactccggtc gcggcgccag gactggctag agcccgctac
     6301 atcgaggggc acgctgcgta tcggtctctc tatccggcgc tccgcccgct cttcccacgg
     6361 gcctgaccgg cggcgaggcc gccggaggtc agatctggat ctcggccagc tcctcgcggg
     6421 aggcctcagc ggccttcttg ccgaggatga tcatggccag gacctcgtcg gtcgtcacac
     6481 tacccttctc aacggtgtcc acgatcctgc cgtgatacat gacgctgatc cgatccgcga
     6541 ggtcgaagac gtcgtgaatg tcatggctga tcaggaagat cccgatgcct tcctccttca
     6601 gttgcttgat cacgtcgcgg acttgcgccg tctccgccgg tccgagggcc gcggtcggct
     6661 cgtccatgat caggatcttg gcctcgaagt ggatcgcccg cccgatggcc acggcctggc
     6721 gctggccgcc agagagggac accaccggcg cggcgaagtt cttgaagttc gggttcaggc
     6781 ggttcaggac cgtcctcgaa gccgcttcca tcgcgtcgtc gtcgagcgag ccgacccggg
     6841 agacgagttc gcggccgagg aagatgttgg ccgggcagtc caggttgtcc gccagggcaa
     6901 gggtctggta gatgcactcg atgccgtagg tcttggcatc gcgcgggctg ctgatcgagg
     6961 ccacctcgcc ccggatgtgg atctcccccg cgtcagcgga ctgagcgccg gagaggacct
     7021 tcatcagggt cgacttgccg gccccgttgc cgccgaccag cccgaccacc tcgccctcgt
     7081 gcaggtcgat gttcacccca tcgacggcgt gcacgccgcc gaaggacacc cggatgtcgc
     7141 gcatgtccac caggggcgcc cgcggcgccg ccgggctagg gggcgtcgac ggcggcagga
     7201 tttcgacggt catcagctcg gatcctaccc ggctcggcgg cgcaggtatg cgtcgaggcc
     7261 cacggcgatg acgaggacga agccgacgat gatgtcctgg gtcggcgagt cgacgttgag
     7321 caggagcatg cccgaccgca gcgactgcat gaccaccgcc cccaggacgg cgcccgggat
     7381 cgagccgatt ccgccggcga acgacgtccc cccgatcacg gccgccgaaa tgacgtcgag
     7441 ctcgttctgc ccgcccaggc cggtgaccgc cgcgttgagg cgagcggtct ggaccgcggc
     7501 gctgatcgcg cacaggacgc ccatcaccgc aaagatcgtg acgatcgtct tgcgggtgtt
     7561 gattccgccc agtcgcgccg cctcggggtt gccgcccatc gcatacacgt tgcggccaaa
     7621 ccggcgccgg gtggcgacat aggtcatcac gatcgtgacc gcgagcaaga tgagcaccgg
     7681 gtaggcgatg ccggtcggaa tgatcaggcc gccgggcggg atcgggatgc cgcgctcctc
     7741 ggcaaactgc gcggcgaggg cctcaggcca ggaataggcg ttcgcgaccc acacggcgcc
     7801 gagcacggcg gcgatggcga acccgttgag cgcgatttcg gcccacatcg gacggacctg
     7861 gaagccgtag tgccgtttcc gccggcgcga gcggacagcg ttgatgacga tcgcgacggc
     7921 aatgacaacg cccaccgccc agctcagggt ctcccccagg gacccctccg gaccgcctcc
     7981 gagaagccgg aagatgccgt ccaggggcgc catggtctgg ccctgcgcgt agctgaagat
     8041 cagtccgcga aagacgagca ggccgcccag ggtgacgatg aatgacggga cgcccccgta
     8101 gccgatgatg agccccgtga tggcgcccag cgtcgccccg agcacgagac ccacgacgag
     8161 ggcgacgagc cacgtgtagg gctggtcgaa cccaaggccg agcgccctgg gaatccactc
     8221 gatctgcacc atggccatcg tgtagccgag gaagccgagc agcgaaccga ccgagaggtc
     8281 gatgttccgc gacacgatga ccagcaccat gccggtggcc atgatcccga tcgaggcgct
     8341 ctgaaccgag aggttccaga ggtttctggc cgtgaggaag ccgccgtccg aaaccacgtt
     8401 gaagccgacc cagatgatcg ccagggccac cagcatgcca aagagacggg tgtcgatctc
     8461 ggcgcggcgg agggcgaccg ggatcctgcg caggaactcg cggatcgccc ctagcacgcg
     8521 ttggtaggcg ggtggctgga gttgggcctc agcaccagtc atggcggatt cctctcaccg
     8581 gggcgatcgg aataggcgag cgccctgcgc cgcggaagcc gcgacgcagg gcgcgtttgg
     8641 ttcagccgga gctcagcgcg tccttacggg cacgcatcca ccgagcctgc ggtcacaccc
     8701 tggcacaggg tctcctcgtc gatccagccg gcgtccacga ccgcgttcag gttgtcctga
     8761 gtgatcgcga gcggagtcag caggatcgag gtcatcgagt tgcccccggg gctgtcgaac
     8821 tgcgcggtgt tcggcacagc cgtgatgtcc ggatcaccgc acagggcaag cgccgcgaca
     8881 ccagctgcgg tgcccaggtc gcgcgcgtcc ttccagacgc tcacgctctg agtgcccagc
     8941 gcgacccggt tcagggcggc cagatcgccg tcctgtccgg acaccggagt tgttccagcc
     9001 atgccctgcg cttcgagcgc agccacaacg ccgcccgcca tgccgtcgtt ctcagacagc
     9061 accgcgtcga cttcgttgtt ggcgtcggtc aggatctgct ccatgttggt ctgggcggtg
     9121 gccggatccc agttgtcggt gtaggtctca ccgacgatgg tgatcgcacc cgaggtaacg
     9181 gcatcgccga tgacctcttc gtagccggct cgcaggaagt cggcgttggc atcagccgag
     9241 ttgcccttga tgatcgcgta gttgccttcg ggaaccaacc cgaagatcac ctcggcctgc
     9301 agacgtccga cgcccacgtt gtcgaaggtg atgtacagcg tagccgggtc ctcgatcagg
     9361 cggtcgtagg cgatgaccgg aacgccctgg tcaatcgcgg tctgcaccga gggcaggatg
     9421 gcctcgccgc tctgggcaag gatgatcagc acgttggctc cctgggagag caggttctcg
     9481 acatcgttgg cctgctgctc ctcggacgag ccagcgtccg ttgagacata ttcggcgttg
     9541 ccggccccca gggcttcctg gatggcgggc tcgtcccact tagcccagcg ctcttcctgg
     9601 tagttgttcc acgacacgcc gacgacgcag tcgccgccag ctgccgcgct ttcaccagcg
     9661 ctcccggcgg cactctccgc ggggctgccg ctcgacgatg gctggcaggc taccagcagg
     9721 atcgcgaata ccgagagtat tgccgctact cggcgaaagg acatgtgctc ctccttcgat
     9781 gtgctgcgct tgaccaatcg gcatccgcca ggctaatcag ccggggcgca gatgccgcgc
     9841 gcgctccccc actcgtgcac tgtacagcct ctcgcggaca cgggctagac cacgcgtcca
     9901 tgcagcgggc tccggccagc cgccgcgccg agctgctgga ggcggtggca accgctaatg
     9961 tctgtcgccg gagccgcatc gtctccgcat gggcgggacg tgagcgcagc cgtgaccggg
    10021 aggacggacc gatggacaga ctgaatggga aggtcgcgct ggtcactggc gcaagccgag
    10081 ggatcggcgc cgcagtggcg cagtcactgg cggacgccgg ggctcgcctg gcgctcggat
    10141 cgcggagcgg ggatgaccct gggatcgagg gcgcgctgac gatgcccgcc gacgtccgag
    10201 atccagcgtc ggtcgaggcg ctggtggccg ccaccgtcga tcagttcggc ggcctcgaca
    10261 tcctcgtcgt gaacgccggc gtcggcgctt acgggccgtt cctcgacctg ccggccgagc
    10321 acatcgagga gatgatcgac gtcaacgtga agggcgccat ctacgccatc cgtgccgcgc
    10381 tccccgtcct gctgaagagc gacgccgccg acatcgtgat gatcgccagc gaggccgggc
    10441 gacgcggcct tccctacgag gcggtctact gcgcctcgaa gttcgcccag gtcggcctga
    10501 cggccgccct ggaccacgag ctgcgggagc agggcgtccg gtgcacgaac gtgtgcccgg
    10561 gtggcgtcgc caccgacttc gcgatggggc ggggccgaac ccccgacatg ccccagctca
    10621 gcgggatgat gcgaccggag gacgtggccg agacggtgct gttcgtggtt accaggccac
    10681 ggacccatcg catcctggag gtggcattcc ggccggtcac cgagacctcc tggggctagg
    10741 atcgatgacc aatctccggt ggggcattct gtcgacggcc gacatcggtc gcacgaaggt
    10801 catcccggga atccagaagg ggaaccggtg cgatgtggtg gccatcgcct cgcgggatga
    10861 gggacgtgct cgcgagaccg cccgtgccct ggggatccca cgggcgcacg ggtcgtacga
    10921 ggcgctcctc gcggacgccg aggtggacgc ggtctacatc ccgctcccca accacctcca
    10981 cgccgaatgg acgctggcgg cggcgcgagc cggcaagcac gtcctgtgcg agaagccgct
    11041 ggccacgacc gcggcggacg ccgagcggat gatcgaggtc tgccgggcgg agggcgtgca
    11101 gctgatggaa gcgttcatgt accggctcca tccgtcgtgg gtggcggtcc gcgagttggt
    11161 cgccggcggg cggatcggtc aactggtggc cgtcgacagc tggttctcgt acttcaacga
    11221 cgatcccgcc aacatccgga acatcgccgc cgtcggtggc ggcgcgctgt acgacatcgg
    11281 ctgctacagc gtgaacctgt cccgcatgct gttcgggtcc gagccgaccg gcatccacgc
    11341 cgtgctccga cgcgaccccg tcggcgggac cgatgtgctg accagcggcc tgctcgagtt
    11401 cggcgacggc gtggccacgt tcacctgctc gacccgagcg gaggacgacc agcgggtcga
    11461 gatctacggg acgaccggcc ggatctcgat cgggatcccg ttcaacatcc cgcccgaccg
    11521 cccgaccgag gtcttcgtga ccgctggcgg caacccgccc gtcgacccgg acaccgaggt
    11581 tctgaccttc gcgacggccg atccctatac cgtcgaggcg gaatatttcg cggcggcggt
    11641 cctcgaggac cgaccggtgc cggtcccgcc agaggacgcc gtcgccaatc tgcacgtcat
    11701 cgagcagatc ctggccgttg cccgcagctg accccggccg ggtggagcgg aggtcatgag
    11761 cgacgacccg tcgggtcggc cagtgcgcgt ccttctgggc gtagccatag cggcgctgct
    11821 gggcctggcc gtcgtagcgc tgctcggtcg ggcgaccttc ggctcgcaac cggaagccct
    11881 gccggcgccc cgtttcatcg acgacaccga tagggctggg ttcgaccatc ggtacgacgg
    11941 cgagttcgcg ttcttcgtcg gcggcggagt cgccgtgttc gactgcgacg gagaccggaa
    12001 gccggacatc tacttcgccg ggggcagcga accagctgcc ctctaccgca acgagagcga
    12061 ggttggcggt ccgttgcggt tcgttccggt ggtgagcccg gtcaccgatc tcgacgacgt
    12121 gaccggcgcc tatccgctgg aggtcgatgg ggacggcgtg atcgacctgg cggtcctgcg
    12181 gcgcggcgga aacgtgctcc tgcgggggat cggggcctgt gcgttcgagg ccgcgaatga
    12241 cgcctggagc ttcgatggcg ggaccgcgtg gaccgctgcc ttcagcgcca catgggagac
    12301 cacgaccgga tggccgacgc ttgcgttcgg gaactacctc gaccagtcaa gcgaaaaccc
    12361 cgagcgcctc tgcgccgaca acgtgctggt tcggccgaaa cccgatggcg ctggtttcgc
    12421 cgcccccgtc ccgttgagtc cgggctggtg ctcgctctcg atcctgttca gcgactggga
    12481 ccggtcgggg cggcgggatc tgcgcgtctc caacgaccgc cactactaca gcgcgaccgg
    12541 cgacggcgag gagcagctgt ggcggatcag ccgggccgag ccgccgcacc tgtacaccga
    12601 ggcggagggc tggcagcggg tccagatctg gggaatgggc atcgcgagct acgacctcac
    12661 cggcgacggc tacccggagt acttcctgac cagccaggcg gacaacaagc tccagaccct
    12721 ggaggacggt cccacggagc cgcgcttccg cgacatggcc ctcgaggcgg gggtcaccgc
    12781 aacccgcccc tacgtcggcg atacgacgct gccgtcgacg gcgtggcacg cggagttcca
    12841 agatgtgaac aacgattcgt tcgtcgacct gttcgtcgga aaaggaaacg tggaggccat
    12901 gccggagtac gcagcggctg acccgagcaa ccttctgatc gggcagaccg acggcacctt
    12961 cgttgaggga gcggagacgg ctggcatcgt cagctacgac cgcgcccgtg gagccgcgct
    13021 cgccgacttc aacctcgatg gcatgctcga cctggtcgtg gtctaccgcc gggaaccggt
    13081 ccgcgtctgg cgcaacgccg gccccgccta ttccgtgggc acggcccagg cgcctgcgag
    13141 tcactggctg gcggtccagc tcggccagga gggaacgaac acgcaggcca tcggggcctg
    13201 ggtcgaggtg cgcatcgggg atcgcgtgat ccagcgagaa atgaccgtgg gcggcggaca
    13261 cgcgagcggg cagctggggt ggacccactt tggcgtcggg ccggccaccg aggtcgaggt
    13321 ccgcgtccag tggccggacg gcgaggtggg gccctggctc ccggtcgacg ccgaccagtt
    13381 cgtcctcgtc gaacgcgacg ccgacgcagc cgagccgtgg gtacccgccg cgggaccaac
    13441 ccagccgtga gccaccaccg gcgtgcgcgc atggccgagc tcagcctccc cgaatttggt
    13501 tcgtcgccga gcacgccggg catcccgacc gagctctacg agcaacgggt cggacgcctg
    13561 cgagcgcgag cggacgaggc ggggttcgac cgactcgtgg tgtacgcgga ccgggagcac
    13621 agcgcgaacc tggcgtatct caccggcttc gatccccgct tcgaggaggc ggtgctcgtc
    13681 gtcgggccgg atgacccgcc cgcgatcctg gtcggcaacg agtgctacgc gatggcgggc
    13741 gcggcaccgc tcgcgatgcg gcgccacctc ctccaggatc tcagcctgcc gtcccagccc
    13801 cgcgaccggt cacggcccct ggccgacctc ctggccgagg agggaatcgg tccggggtcc
    13861 cgggttggcg ttatcggttg gaagacatac ggcgacgccg aggccagcga cgcgccggcg
    13921 tacctggtcg acgagatccg gcgtctgacc ggccccgcgg gatcggtcac gaacgcgacc
    13981 gggctgctca tcgacgccgg agacgggctg cgggtcatga acgaggtcga gcagctcgct
    14041 gccttcgagc atgcagcgtg ccacacctcg agcggggtcc gccgcctgct gttcggcctg
    14101 cagccgggaa tgaccgagca ggatgcggtc ggcctcctgg cgtggaacgg ctcccctctc
    14161 tcctgccacc tcatgctctc gagtgggccc cgcgccacgc tcggcctact tagcccgggc
    14221 gatcgcacc
//
LOCUS       DASYGU010000089         9773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_888939, whole genome shotgun sequence.
ACCESSION   DASYGU010000089 DASYGU010000000
VERSION     DASYGU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888939"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGO32_02295"
     CDS             <1..430
                     /locus_tag="VGO32_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV7199615.1"
                     /translation="SLVEFVDGSYKAQLGLPDMRLPIQYALSYPERHPGPARTAAPED
                     WPPLTFAPLDPVRFPAYATVRAAAEAGGNRGTVVNAADEVAIAAFLNGALPFPAIATT
                     IEQAVNRWGNDSEPSLAEIQDTDREVRTALEAELGIGATA"
     gene            430..1554
                     /locus_tag="VGO32_02300"
     CDS             430..1554
                     /locus_tag="VGO32_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEV7199616.1"
                     /translation="MDVVLTILAFLAIIVVLVVVHELGHFAVAKWSGVAVQEFGVGFP
                     PRIGSVVFRGTRYSLNWIPLGGFVKMLGEDGEIEVERLRERGLSDTAIEKVMEGALSR
                     RRIPVRLAVLLAGVAMNFVVGALLFSMAYSQPTPEYHGPVTVTTIQSGSPADGVLEEG
                     DVITGADGQTFELSADMTAYVREQAGEPVTLQIMRGTEQLDIEVTPRVLTDEQVQAGE
                     GAVGFGWESEEIVMGPPLATGPVDALAQGFSTAARVAAEIPGGLLRAVAGILGLAPST
                     GDVAGPIGIAQQTGRLLDAPLVSQLFFIGLLSINLAVLNTLPFPPLDGGRVLIVLIEA
                     IRRRKMSAEREALIYFTGFVVLIALVILISIQDIARLPGS"
     gene            complement(1739..2824)
                     /locus_tag="VGO32_02305"
     CDS             complement(1739..2824)
                     /locus_tag="VGO32_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEV7199617.1"
                     /translation="MRRSAAGWLRTAIPVIVLGVVLTTLMLLGTVALRSAGVVCAATA
                     GCPNPPGPPAYGPAAGSVYRLAGPDRYRTAAAVSMDQWSPGVYGLFVASGENFPDGLA
                     AAAAAGARGIPLLLVRRDSIPAEIAAELERLDPGWIWVIGGTSVVSLAVEQALAAYTS
                     GEVERLWGADRYATAVAISERLFSATEEVFIATGTSFPDALVGSAAAGYLEAPLLLVK
                     RDTIPSVVVQDLERLIPQKIIILGGTGVISSQVEAALQAYAPDVTRIGGVDRYDTAVK
                     ISEATFTFGSQRVYVVTGKNFPDALVACAAAGDWNSPVLLVPGTSLPYPVATEVTRLD
                     PDSAVVVGGTSAVSPAVEQALIPLVGP"
     gene            complement(2915..3211)
                     /locus_tag="VGO32_02310"
     CDS             complement(2915..3211)
                     /locus_tag="VGO32_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199618.1"
                     /translation="MRRVAVLGIAAVLVVAFAVLWVVPVVAGPTIDCGPFDEATCERI
                     VAEIIASHDDPAEVLPDSYRPPFLPVTSVVLSWTEGSVDYEIHWLFGGVGVISD"
     gene            3304..3498
                     /locus_tag="VGO32_02315"
     CDS             3304..3498
                     /locus_tag="VGO32_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199619.1"
                     /translation="MRAWLVKYKIPVAAISGTILGLVLFIGPGHAELNPANVILATSC
                     GAIFGVSWWAAARSYEPPEE"
     gene            3576..4082
                     /locus_tag="VGO32_02320"
     CDS             3576..4082
                     /locus_tag="VGO32_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199620.1"
                     /translation="MAERWLCKRCFTTADESVEACPNCGLARGAEAPSAAASTIPEDE
                     EPIKAAAQPPPESRVAFVPLNAPLPPIPRPSTSCQQCGSPLFAQWWNPTKSGYECRRC
                     GSLTVGQPPAGATKARTGCFIGCAAIIGFFGILAGIGGNFQGGVGLVAFAGLVGIAGL
                     LLSRRSTG"
     gene            4248..5387
                     /gene="nusA"
                     /locus_tag="VGO32_02325"
     CDS             4248..5387
                     /gene="nusA"
                     /locus_tag="VGO32_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEV7199621.1"
                     /translation="MNRDFIGALLQLNAEKGVPQDILLQTLEQAIESAYRRNAEAPEN
                     VVVKIDPETGEISVFREYEVVAGNEEVTDPELQKTVAEAVAIEEGAGVGSRVRVPENV
                     TQQQLGRIAAQTAGQVYRQRLREAERDVVYHQYVSREGEILTGVVTRTTESAVVLDMS
                     RGVEAVLSVSEQLPGEHYRIGQHLKVFVLEVRRTTRGPVLVVSRTHRGFLRRLMEMEI
                     PEIYNGSVVIRGIAREAGSRSKVAVESRQPGVDAKGAAVGQRGTRIQAIVAELNGEKV
                     DVVLWNEDASQFVAEALSPAEVIEVRIDEEHRIANVIVPERQLSLAIGREGQNARLAA
                     KLTGWRIDIRSDAGVAAAGAGGTRTATSSEDGEAAEVAEDAEATA"
     gene            5368..5613
                     /locus_tag="VGO32_02330"
     CDS             5368..5613
                     /locus_tag="VGO32_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV7199622.1"
                     /translation="MPKRRPDHQPQRTCAVCRAVHTKRDMQRIVRTADGEAVMDASGR
                     LPGRGTYVCSDPACQTNPSRDKAIARALGIGVIHATA"
     gene            5600..7483
                     /gene="infB"
                     /locus_tag="VGO32_02335"
     CDS             5600..7483
                     /gene="infB"
                     /locus_tag="VGO32_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV7199623.1"
                     /translation="MPPRRYTQNRRGPRPPRKNRESTSSTETATLPRPSGPVEVGTTV
                     VVQDLAAALGVSTAAVISELIRNGIFATINQSIDYDTASLVAAEMGMELVAKGAAEAE
                     GEAQELVAPGGAEDGVRITPWGEDDPANLITRAPIVTIMGHVDHGKTSLLDAIRDTAV
                     ASGESGGITQHIGASEAVHGERRIVFLDTPGHEAFTAMRARGAQVTDIAVIVVAADDG
                     VMPQTKEAIDHVRAARVPMIVAVNKIDKPDANPDRVKQELADNGVLIEEYGGEIPAVA
                     VSAKQRQGIDDLLEVIGLVADLQDLKANPNREAYGTIIESKIEKGRGNVATVLVQTGT
                     LKVGDIVTVGKTYGRVRALQNAAARRVKEAGPATAVEIIGLQELPEAGDLLRVVPDEK
                     AARDAAEAVERHAAGSGASSLEELSAQIQSSEVKELRVILKTDVQGSLGAIRHALDAL
                     STTETRINILSEGAGDINESDVLLAAASNAVVVGFNTKLDPGAERAVEASGVDVRLYE
                     VIYKLTDDIQLALQGLLTPKLVEQIEGHAEVRQLIKAGKAGTIAGSYVLDGRMVRGGQ
                     ARLMRGGQLIVESRIESLRRFKDDVREVAASFECGIGLAGSPEYAEGDVIECFQMVSE
                     GSV"
     gene            7396..7836
                     /gene="rbfA"
                     /locus_tag="VGO32_02340"
     CDS             7396..7836
                     /gene="rbfA"
                     /locus_tag="VGO32_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV7199624.1"
                     /translation="MRHRPRRLAGIRRGRRHRVLPDGVGGVRLSKRTDRLDSQIRAEL
                     MQIFQRDMADPRVGFATVTRVHTSPDLTLAQVWVSVYGPDSDMSTTVNALTDAAGWLR
                     RRLGERMTMRKVPRLEFKADDSIASGDTVLRILRDLDTDSGADR"
     gene            7833..8810
                     /locus_tag="VGO32_02345"
     CDS             7833..8810
                     /locus_tag="VGO32_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV7199625.1"
                     /translation="MKEVVDAIRGAQRITVIGHENPDADTLGAALAIRIVGERLGIPT
                     EVVMADPVPIYLQFLPRVDEVKTTPGFEPDVAVIVDSGDLARTGTIATDHRAWLEQSR
                     IVNIDHHVSNPGFGAVNLVDPDAASTCEMVTFLLAELGVELDRDLATVLLAGIVNDTH
                     TFAHPNVTPRTLRAAASLVEAGASLAAINRAIYADKPNSTIAVWGRILAGVGFASGGR
                     IVHAALTEAMLTETGATRESSEGFIDLLSLSREADIVLLFKEQGQTQTRVSIRTTAVA
                     DAVAITSVFGGGGHARAAGCTVAAPIADARTQVLAECERELARADDRRH"
     gene            8776..9666
                     /gene="truB"
                     /locus_tag="VGO32_02350"
     CDS             8776..9666
                     /gene="truB"
                     /locus_tag="VGO32_02350"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV7199626.1"
                     /translation="MSSPAPMIGVINLDKPVGPTSHDMVGLVRRLTGLRRIGHAGTLD
                     PLASGVLPILVGPATRLSQELTGGRKRYDAVVRLGFRSQTDDAQGPIEPGGAPPDGDA
                     VRAALPEFEGTYEQRPPAFSARKAGGVTAYEAARKGSALDLAPREITIHAIRLLDMTP
                     ADGWLDVHLDLETGPGTYIRSVARDLGERLGCGGYLHALRRTEAAGLLAAEGMTPDGL
                     EAVAARGELAEALIPVERLLGLETVTLEAEGAERFTHGGELAWPGDGRRQVHGPSGLL
                     GIGELRDGRLHPRTVLVEGA"
BASE COUNT         1619 a         3360 c         3365 g         1429 t
ORIGIN      
        1 ctccctcgtg gagttcgtcg acggctcgta caaggcccag ctcggcctgc ccgacatgcg
       61 gctcccgatc cagtacgcgc tgtcctaccc cgagcgccat cccggcccgg cccggacggc
      121 cgctcccgag gattggccgc cgctgacctt cgcgccactc gatccggtgc gtttccccgc
      181 gtatgccacc gtgcgcgccg ccgccgaggc cggtgggaac cgggggaccg tcgtgaacgc
      241 cgctgacgag gtcgccatcg cggccttcct gaatggcgcg ctgccgttcc cggccatcgc
      301 gacgacgatc gagcaggccg tgaaccggtg ggggaatgac tcggagccca gtctggccga
      361 gatccaggac accgaccggg aggtccggac cgcgctcgag gccgagctcg gcatcggagc
      421 caccgcctga tggacgtcgt cctcaccatc ctggccttcc tggccatcat cgtcgtcctg
      481 gtcgtggtcc acgagctcgg ccacttcgcg gtggccaagt ggtccggggt cgcggtccag
      541 gagttcgggg tcggctttcc gccgcgcatc ggatcggtcg tgttccgcgg aacgcgctac
      601 tcgctgaact ggatcccgct cggcggcttc gtgaagatgt tgggcgagga cggcgagatc
      661 gaggtcgagc gcctccgtga gcggggcctg tccgacaccg ccatcgagaa ggtcatggaa
      721 ggcgcgctca gccgccgccg gatccccgtc cgtcttgccg tgctgctggc cggcgtagcc
      781 atgaacttcg tcgtgggcgc gctgctgttc tccatggcct acagccagcc gacacccgag
      841 taccacggcc ccgtaacggt cacgaccatc cagtccggaa gcccagccga tggcgtcttg
      901 gaggaaggcg acgtcatcac cggcgccgac gggcagacct tcgagctgtc ggccgatatg
      961 accgcctacg tccgtgagca ggcaggcgag ccggtcacgc tccagatcat gcgaggcacc
     1021 gagcagctcg acatcgaggt cacgccacga gtcctgaccg atgagcaggt ccaggccggc
     1081 gagggtgccg tcggcttcgg ctgggagtcg gaggagatcg tgatgggccc gccgttggcc
     1141 accggtccgg tggacgccct ggcccagggg ttctcgaccg cggcccgcgt cgccgcggag
     1201 atcccgggcg gcctcttgcg cgccgtcgcc ggcatcctgg gccttgcccc ctcgaccggg
     1261 gacgtcgccg gaccgatcgg catcgcccag cagaccggtc ggctgctgga tgcgccgctc
     1321 gtgagccagc tgttcttcat cggcctgctg tcgatcaacc tcgccgtcct gaacacgctg
     1381 ccgttcccgc cgctcgatgg cggccgtgtc ctcatcgtgc tcatcgaagc catccgccgc
     1441 cgcaagatgt ccgccgagcg cgaggcgctc atctacttca ccggcttcgt ggtcctcatc
     1501 gccctcgtca tcctgatctc gattcaggac atcgcgaggc tccccgggtc ctgatctgag
     1561 cacgaaagag ccctccggct aggcgctcgg agggctatgt accacccgct gtaccacttt
     1621 cagacgccgc gtcctggagc tcgtcgggat cagcttcacc gccgatgagc aagcgatgat
     1681 ctactaggcc caggccatct gggacgagcc cggccccggt cactagtaac gcgtggaatc
     1741 aggggcctac gaggggaatc agggcctgct cgactgcggg agacaccgca gacgttccgc
     1801 cgaccacgac cgccgaatcc ggatccaacc gtgtgacctc ggttgcgacc ggatagggga
     1861 gggaggtgcc gggcacgagc aagaccgggc tgttccagtc gcccgccgcc gcgcacgcca
     1921 cgagagcgtc cgggaagttc ttgcccgtaa cgacatacac ccgctggctc ccgaacgtga
     1981 acgtggcctc agagatcttc accgcggtgt cgtagcgatc caccccgcca atccgagtga
     2041 catccggggc atacgcttgc agggccgctt ccacttgcga ggagatcaca ccagttccgc
     2101 cgaggatgat gatcttctgg gggatcagtc gttccaggtc ctggacgacc acggagggga
     2161 tcgtatctcg cttcacgagt aacaagggcg cctcgaggta gccggcggcg gcactcccga
     2221 ccagggcatc cgggaagctt gtgcctgtgg cgatgaagac ctcttccgtc gccgagaaga
     2281 ggcgctccga gatggccacc gcggtcgcgt accgatccgc tccccacagc cgttcgacct
     2341 cgccggaggt gtaggcagcg agtgcctgct cgaccgccaa cgagacgacc gacgtgccac
     2401 cgatgaccca gatccagcct gggtcgagcc gctccagctc ggccgcgatc tcggccggta
     2461 tcgaatcgcg ccgcacgagc aacaggggga tcccgcgggc tcctgccgcg gccgccgccg
     2521 cgaggccgtc ggggaagttc tcgccactgg cgacgaacag gccgtacacc ccgggcgacc
     2581 actgatccat cgacaccgcg gcagcggttc gatagcgatc tggaccagcc agtcggtaga
     2641 ccgaaccggc ggcagggcca taggcgggcg gccctggcgg gttgggacag cccgcggtcg
     2701 cggcgcatac cacgccggcc gacctcagcg ccacggtgcc aaggagcatc agggtcgtca
     2761 gaacaacgcc gagaacgatt accggaatcg cagtccgaag ccagccggcc gccgaacgtc
     2821 tcatggccga agtctaggca gtcacccggc cgcaaggaat aggacccgcg gtagccgagg
     2881 tgggcgtcgg gagccggttg ggtatgttcg cgccctagtc gctgatgacg ccgaccccgc
     2941 cgaagagcca atggatctca tagtcgactg agccctcggt ccagctcagc accacactcg
     3001 tgacgggcag gaagggcggg cggtaagagt ccgggaggac ctcggcgggg tcgtcatgac
     3061 tcgcgattat ttccgccacg atccgctcgc aggtcgcctc gtcgaatggt ccgcagtcga
     3121 tcgtcgggcc tgctacgaca ggcacgaccc acaagacggc gaaggccacc acgaggacgg
     3181 ccgcgatccc gagtaccgct acgcgacgca tctcgccgag catcgtacgg tccgaatctc
     3241 gatccaggac atcgcccgcc tgccggggag ctgatcggat gctcgacgcc tagaattcga
     3301 cgcgtgcgag cgtggctggt caaatacaaa attccggtcg cggcgatcag cgggaccatc
     3361 ctgggcctcg tgctcttcat aggtcccggt catgccgagc tgaaccccgc gaacgtcatc
     3421 ctcgccacct cgtgcggagc gatatttggc gtctcctggt gggccgcggc ccggagctac
     3481 gagccgccgg aggagtaaat gccggagcct gaacgggcaa acccggatcg gccgtaaggt
     3541 aggaccccgc cgactcacgg agtactctga agtcgatggc ggagcgctgg ctctgcaaac
     3601 gctgcttcac gacggcggac gagagcgtcg aggcctgtcc gaactgcgga ctggcccgtg
     3661 gcgccgaggc cccgtcagcc gccgcctcca ccattcccga ggacgaagag cccatcaagg
     3721 cggcagcaca accgcctcct gagtcacgcg tggcattcgt tccgctaaac gctccgctgc
     3781 cgccgattcc ccggccttcc accagctgcc agcaatgcgg aagcccgctg tttgcccaat
     3841 ggtggaatcc aacgaagagc ggctacgagt gccgccgatg cgggagcctc accgtggggc
     3901 aaccaccggc cggcgccacc aaggccagga ccggctgctt catcggatgc gccgccatca
     3961 tcggcttttt tgggatcctg gccggtatcg gcggcaactt ccagggcggc gtgggtctgg
     4021 ttgcgttcgc tggcctggtg ggaatcgctg gactgctcct gtccaggcgt tccaccggat
     4081 agctggcgcg cccgaaccag gcgtggaatt ggcgatttca aggtccgctg atatactcgc
     4141 cacggtcaac ggaccgggtg tggaccaaac cgataagcac ttgatcttgg acgtgggtcg
     4201 gtctgccaac ccacgttctg tttttcttcg ggcagggagg tgccagcgtg aatcgcgact
     4261 tcatcggcgc cctgctgcaa ttgaacgccg agaagggcgt tccccaggac atcctcctcc
     4321 agacgctcga gcaagcgatt gaatcggcct accggcgaaa tgccgaagcg cccgagaacg
     4381 tcgtggtcaa gatcgacccc gagacggggg agatcagcgt ctttcgcgag tacgaggtcg
     4441 tcgccggcaa cgaggaagtg accgatccgg agctccagaa gacggtcgcc gaggcggtcg
     4501 cgatcgagga gggcgccggg gtcggcagcc gcgtccgggt cccggaaaac gtcacccagc
     4561 agcagctcgg ccgaattgcc gcgcagaccg cgggccaggt ctatcgccag cgcctccgcg
     4621 aggcggagcg cgacgtcgtg taccaccagt acgtcagccg cgagggcgag atcctgaccg
     4681 gggtcgtgac ccgcaccacc gagtccgcgg tcgtgctcga catgagccgc ggcgtggagg
     4741 cggtcctctc ggtcagcgag cagctgcccg gggagcacta tcgcatcggc cagcacctga
     4801 aggtcttcgt gctcgaggtc cgtcggacca cgcgcggccc ggtcctggtc gtaagccgaa
     4861 cccaccgcgg cttcctgcgg cggctgatgg agatggagat tcccgagatc tacaacggct
     4921 cggtcgtgat ccgcggcatc gcccgcgagg ccggcagccg ctcgaaggtc gcggtcgaga
     4981 gtcgccagcc gggcgtggac gccaagggtg ccgcggttgg ccagcgggga acccggatcc
     5041 aggccatcgt ggccgagctg aacggcgaga aggtcgacgt cgtgctgtgg aacgaggacg
     5101 ccagccagtt cgtggccgag gcgctctcgc cggccgaggt catcgaggtc cggatcgacg
     5161 aagagcatcg cattgcgaac gtgatcgttc cggagcgtca gctgtcgttg gccatcggcc
     5221 gcgagggaca gaacgcccgg ctggcggcca agctaaccgg ctggcggatc gacatccgct
     5281 ccgatgcggg cgtggccgcg gccggggccg gaggaacgcg gactgccacc tccagcgaag
     5341 acggggaagc tgcggaggtc gccgaagatg ccgaagcgac ggcctgatca ccagccgcag
     5401 cgcacctgcg cggtctgccg cgcggtgcac accaagcgag acatgcagcg catcgtccgg
     5461 accgcggacg gtgaggcggt gatggacgcc agcgggcgct tgcccgggcg tggcacgtac
     5521 gtgtgctccg atcccgcctg ccagactaac cccagccgcg acaaggccat tgcccgcgca
     5581 ctggggatcg gagtgataca tgccaccgcg taggtacacc cagaaccgcc gcggtccgcg
     5641 accgccgcgc aagaaccggg agtccacgag cagcaccgag accgccacgc tgccacgccc
     5701 gtctgggccg gtcgaggtgg gaaccaccgt ggtcgtccag gacctggccg cggcgctcgg
     5761 agtcagcacc gccgcggtca tcagcgagct gatccggaac gggatcttcg ccaccatcaa
     5821 ccagtcgatc gactacgaca ccgcctcgct ggtcgccgcc gagatgggca tggagctcgt
     5881 cgccaagggc gccgcggagg ccgagggcga agcgcaggag ctggtcgcgc cgggtggcgc
     5941 ggaggacggg gtccggatca cgccatgggg cgaggacgat cccgccaacc tgatcacgcg
     6001 cgccccgatc gtcacgatca tgggccacgt ggaccacggc aagacgagcc tgctggacgc
     6061 catccgcgac acggccgtcg cgtccggcga gtcgggtggc atcacccagc acatcggtgc
     6121 ctcggaggcg gtccacgggg agcggcggat cgtgttcctg gacacccctg gtcacgaggc
     6181 gttcaccgcc atgcgggccc gtggcgccca ggtgaccgac atcgcggtca tcgtggtcgc
     6241 ggccgacgac ggagtcatgc ctcagaccaa ggaagcgatc gaccacgttc gcgcggcgcg
     6301 cgtccccatg atcgtggcgg tcaacaagat cgacaagccc gacgccaacc ccgaccgggt
     6361 gaagcaggag ctggccgaca acggggtcct gatcgaggag tacggcggcg agatcccggc
     6421 cgtggcggtg tccgccaagc agcggcaggg gatcgacgac ctgctggagg tcatcggcct
     6481 ggtggccgac ctgcaggacc tgaaggccaa cccgaaccgc gaggcgtacg gcacgatcat
     6541 cgagtccaag atcgagaagg gccggggcaa tgtggccacc gtcctggtcc agaccggcac
     6601 gctgaaggtc ggcgacatcg tgaccgtggg caagacctac ggccgcgtcc gcgccctcca
     6661 gaatgcggcc gcccgccggg tgaaggaggc agggcccgcc accgcggtcg agatcatcgg
     6721 cctccaggag ctgcccgagg ccggcgacct gctgcgggtc gtgcccgacg agaaggccgc
     6781 ccgcgatgcc gccgaagcgg tcgaacgcca tgcggccggg tccggcgcgt ccagcctcga
     6841 ggagctcagt gcccagatcc agtcgtccga agtcaaggag ctgcgcgtca tcctcaagac
     6901 cgacgtccag ggatcgctgg gcgccatccg ccatgcgctc gacgcgctga gcacgaccga
     6961 gacccggatc aacatcctca gcgagggggc gggggacatc aacgaatccg acgtcctgct
     7021 cgcggccgcc tcgaacgcgg tggttgtggg cttcaacacc aagctcgacc ccggcgccga
     7081 gcgcgcggtg gaagcttcgg gcgtggatgt gcgcctgtac gaggtcatct acaagctgac
     7141 cgatgacatc cagctcgccc tccagggcct cctgaccccg aagctggtcg agcagatcga
     7201 gggccatgcc gaggttcgcc agctgatcaa ggccggaaag gcgggcacta tcgccggctc
     7261 atacgtgctc gacgggcgga tggttcgcgg gggccaggcc cggctcatgc ggggtggcca
     7321 gctgatcgtc gagtcgcgga tcgagtccct gcgccggttc aaggacgacg tgcgcgaggt
     7381 ggcggccagc ttcgaatgcg gcatcggcct cgccggctcg ccggaatacg ccgagggcga
     7441 cgtcatcgag tgcttccaga tggtgtcgga ggggtccgtc tgagcaagcg caccgatcgg
     7501 ctcgacagcc agatccgagc cgagctgatg cagatcttcc agcgtgacat ggccgacccg
     7561 cgggtcggct ttgcgacggt cacccgcgtt cacaccagcc cggacctgac ccttgcccag
     7621 gtctgggtat cggtctacgg ccccgattcc gacatgagca ccaccgtgaa cgccttgact
     7681 gatgccgccg gctggctccg ccgccggctc ggtgagcgga tgacgatgcg gaaggtgccg
     7741 cggctggagt tcaaggccga cgactcgatc gcaagcggcg acaccgtcct ccgcatcctc
     7801 cgcgacctcg acaccgactc gggtgcggac cgatgaagga ggtcgtcgat gccattcgcg
     7861 gggcgcagcg gatcacggtg atcggtcacg agaatcccga cgccgacacc cttggcgccg
     7921 cgcttgcgat ccggatagtg ggggagcggc tcgggatccc gaccgaggtg gtgatggcgg
     7981 acccggttcc gatctacctc cagttcctgc cccgggtcga cgaggtgaag accacccctg
     8041 gattcgagcc ggacgtggcg gtgattgtgg attccggcga cctggcccgg accgggacga
     8101 tcgccaccga ccaccgggcg tggctggagc agtcgcggat cgtgaacatc gaccatcacg
     8161 tcagcaaccc cggcttcggt gcggtgaatc tggtcgatcc ggatgccgcc tcgacctgcg
     8221 agatggtgac cttcctcctc gccgagctgg gcgtggagct ggaccgcgac ctcgccaccg
     8281 tgctcctggc cgggatcgtg aacgacaccc acaccttcgc ccaccccaac gtcaccccgc
     8341 gcacgctccg ggcggccgca tcgctggtcg aggctggggc gtcactggcg gccatcaatc
     8401 gcgcgatcta cgctgacaag ccgaactcca cgatcgcggt ctggggtcgg atcctggccg
     8461 gagtggggtt cgcgtccggg ggccggatcg tccacgcggc cctgaccgaa gcgatgctga
     8521 ccgagaccgg cgccacgcgc gagtcatcgg aagggttcat cgacctcctg agcctgtccc
     8581 gcgaggccga catcgtcctc ctgttcaagg agcagggcca gacccagacc cgggtcagca
     8641 tccgcaccac ggctgtggcc gacgcggtcg ccattacatc ggtctttggc gggggaggcc
     8701 atgcccgggc ggcgggctgc acggtggccg cccccatcgc cgacgcccgg acccaggtcc
     8761 tggccgagtg cgagcgtgag ctcgcccgcg ccgatgatcg gcgtcattaa cctcgacaag
     8821 ccggtcggcc ccacctcgca cgacatggtc ggcctcgtcc gacggctgac cgggctgcgc
     8881 cggatcggtc acgctgggac cctggacccc ctcgcctcgg gcgtgctgcc gatcctggtc
     8941 ggcccggcca cgcggctgag ccaggagctg accggcgggc ggaagcggta cgacgcggtg
     9001 gtccggctgg gcttccgttc acagaccgat gacgcccagg gaccgatcga gccgggtggc
     9061 gcaccgcccg acggggatgc ggtgcgggcg gcgctccccg agttcgaggg cacgtacgag
     9121 cagcggccgc cggcgttctc cgcgcggaag gccgggggcg tgaccgccta cgaggcggcg
     9181 cggaagggct cggcactgga cctggcgccg cgcgagatca cgatccacgc catccggctc
     9241 ctggacatga cccctgcgga tgggtggctc gacgtgcacc tcgatcttga gaccggcccg
     9301 gggacctaca tccgctcggt ggcccgcgac ctcggggagc ggctgggctg cggcggctat
     9361 ctccacgccc tgcgccggac cgaagcggcc gggctgttgg cggccgaagg tatgacaccc
     9421 gatggcctcg aggcggtggc cgcgagggga gagctggccg aggcgctcat tccagtcgag
     9481 cggttgctcg gtctcgaaac ggtcactctg gaggcggagg gcgccgagcg cttcacccac
     9541 ggcggcgagc tggcgtggcc cggcgacggc cgccgccagg tccatggacc atccggcctg
     9601 ctgggcatcg gcgagctccg tgacggccgg ctccatcccc gcaccgtcct ggtcgagggc
     9661 gcatgacgac cgccgcacca caccgggccc tcagcctgga cgaggtcgcc gcggcgtcga
     9721 tcggacccgc ggcggtggcg ctgggcgtat tcgacggcgt tcatcgcggg cac
//
LOCUS       DASYGU010000090         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_323884, whole genome shotgun sequence.
ACCESSION   DASYGU010000090 DASYGU010000000
VERSION     DASYGU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323884"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..980
                     /locus_tag="VGO32_02355"
     CDS             129..980
                     /locus_tag="VGO32_02355"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199627.1"
                     /translation="MVANKPEAPVVRLTGVSKSFAPAGSSEAPVVALSDISMDIHRGE
                     FVSLIGPSGCGKSTLLRLIGDLTEPSGGTVEINGKSAAQARLDREYGMVFQSPVLFDW
                     RTVEDNVRLPLELFGFSKSAQKEKIREMLDLVRLTDFAGHRPYQLSGGMQQRVAIARA
                     LAFSPSLLLMDEPFGALDEMTRERMNSEVLSIWARTGITVIFVTHSIPEAVFLSSRVA
                     VMSARPGRITRLVDVELPRPRGLDTRESPAYFSHITEVREALRAGEGDETDIASAERT
                     AAEGLVG"
     gene            981..1766
                     /locus_tag="VGO32_02360"
     CDS             981..1766
                     /locus_tag="VGO32_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199628.1"
                     /translation="MQAIRRYVPAALILVGVIVAWEVLVQVSDVQGFILPAPSAIGAA
                     LADNWTEGYAILPAAQVTLFEAMGGLILGTVLGVATAFVVSWFPASRDTVLPLAIAIN
                     AVPIIAFAPVANNWFGLLSPLSKMAIAATLVYFPIMISVLRGLTQVEPSALELMRSYG
                     AGPRVVTREVRVPNALPFFLTGLKVATTLSLIGAVVGEYFGGLSATLGRVVVAAASGL
                     KFDVTWAAILVVSLVGIVMYLMVVLLERLLIPWHPSVRGDRPA"
     gene            1872..3056
                     /locus_tag="VGO32_02365"
     CDS             1872..3056
                     /locus_tag="VGO32_02365"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7199629.1"
                     /translation="MRVTRVFGVLVVAASLFLAACQSSASESPAESAAGSAGASAEPS
                     QVTYEPASVSLQLQWTDQAQFAGYYAAVEQGYYDDVALTVEILPGGGTIVPQQVGSAP
                     DGPEFTIAWMPKVLEAREAGSDLVHLAQVFQRSATLSVAWADAGITEVTDWAGKRIGA
                     WPFGNELEVLATAEILGGLTDGEDFTRVEQDFDMNELIAAQDGCAASDSDCVDVAEAM
                     IYNEWAQLLETTDPNTGELFQPDQFEVFDFNELGTAMLQDGLYARQSWLDEGNNTEVA
                     MRFLQASFRGWMYCRDNPDDCVQYSIDRGSIWEAGHLAWMMNEINKLVWPAEAGIGHL
                     DEGELTQTVDTASAAGILTGPPDDAAFNSAIWEEALSGIDGDTTGDDFAAPEVPITPG
                     GE"
BASE COUNT          482 a         1035 c         1070 g          522 t
ORIGIN      
        1 tacaccgtgg cccccgaaaa gctgtggggc gcgatcctgt tctgctcatt tgtcggcgtc
       61 gccgcattcg ggctcgtgcg cctggtggag atgcgggtcg tccgcgtgcg gccgggaatg
      121 gaaccctgat ggtcgcgaac aagcccgagg cgccggtcgt gcggctgacc ggggtgtcca
      181 agtcgttcgc gcctgccggg tcgtcggagg cgccggtcgt ggccctcagc gacatcagca
      241 tggacatcca tcggggcgag ttcgtctcgc tcatcggtcc ctccggctgc ggcaagtcca
      301 ccctcctccg gctcatcggg gatctcaccg aaccgtcggg cgggacggtc gaaatcaacg
      361 gcaagagtgc cgcccaggca cggctggacc gcgagtacgg gatggtcttc cagtccccgg
      421 tgctgttcga ctggcgcacg gtggaggaca acgtccgcct gccgctcgag ctgttcggct
      481 tcagcaagtc ggcccagaag gaaaagattc gcgagatgct ggacctggtc cggctgaccg
      541 acttcgccgg ccatcggccc tatcagctct cgggcgggat gcagcagcgg gtggccatcg
      601 cccgggcgct cgccttctct ccatcgctcc tcctgatgga cgagccgttc ggcgccctgg
      661 acgagatgac ccgggagcgg atgaactcgg aggtgctcag catctgggcc aggaccggga
      721 tcacggtcat cttcgtgacc cactccattc ccgaggccgt gttcctgtcg agccgggtgg
      781 cggtcatgag cgcgcgtccg ggtcgcatca cccggctggt agacgtcgaa ctgccgcgcc
      841 cgcgtggcct ggacacccgc gagagccccg cctacttctc ccacatcacc gaggttcgcg
      901 aagccctccg ggccggcgag ggcgacgaga ccgatatcgc ctccgccgag cgaaccgcgg
      961 ccgaagggct cgtcggctag atgcaggcca tccgccgcta cgtcccggcc gcgctgatcc
     1021 tggtcggtgt gatcgtggcg tgggaggtct tggtccaggt ttccgacgtc caggggttca
     1081 tcctgcctgc cccgtcggcc atcggggccg ccctggccga taactggacc gagggctacg
     1141 ccatcctgcc cgccgcccag gtgacgctct tcgaggccat gggcggtctc atcctgggca
     1201 cggtgcttgg tgtggccacc gcgttcgtcg tctcctggtt ccccgcctcg cgcgacacgg
     1261 tcctgccgct cgcgatcgcc atcaacgcgg tgccgatcat cgcgttcgcg ccggtggcca
     1321 acaactggtt tggcctcctc agcccgctgt cgaagatggc gatcgcggcg accctcgtct
     1381 acttcccgat catgatcagc gtcctccgcg gcctgaccca ggtcgagccc tcggcgctgg
     1441 agctgatgcg gtcctacggg gccgggccac gggtcgtgac ccgcgaggtc cgcgtcccga
     1501 atgccctccc gttctttctg accgggttga aggtggcgac caccctgagc ctcatcgggg
     1561 cggtggtggg cgagtacttc ggcggcctgt cggccaccct cggacgggtc gtggtcgcgg
     1621 cggccagcgg cctgaagttc gacgtgacct gggcggccat cctggtcgtg tcgctggtcg
     1681 gcatcgtgat gtacctgatg gtcgtcctcc tcgaacgact gctcatcccg tggcacccgt
     1741 ccgttcgcgg cgacaggccc gcgtagcagg caggctcggc gcggaccggg ttccgagcag
     1801 cgaggctgag tagaatcagt ggtcacggat ggcccctgcc ctgccccggg ccgtcacatt
     1861 gaggagtgga gatgagggtc acacgagtct ttggcgtgct ggtcgtcgcg gccagcctgt
     1921 tccttgccgc atgtcaatca agtgcgagcg agagtccggc ggagagcgcc gcgggaagcg
     1981 ccggcgcgag cgccgagccg agccaggtca cctacgagcc ggccagcgtc agcctccagc
     2041 tgcaatggac cgaccaggcc cagttcgcgg ggtattacgc cgcggttgag cagggctact
     2101 acgacgacgt cgccctgacg gtcgagatcc tgcccggtgg cgggaccatc gttccgcagc
     2161 aggtaggttc ggctccggat ggtccggagt tcacgatcgc ctggatgccc aaggtcctcg
     2221 aggcacgaga ggccggatca gacctcgtcc acctggcgca ggtcttccag cggtccgcca
     2281 ccctctcggt ggcatgggcc gacgcgggca tcaccgaagt gacggactgg gcgggcaagc
     2341 gcatcggcgc ctggccgttc ggaaacgagc tcgaggtcct ggcaaccgcc gagatcctgg
     2401 gcggcctgac cgatggtgag gacttcaccc gggtcgagca ggacttcgac atgaacgagc
     2461 tcatcgcggc ccaggatggg tgcgcggcga gcgacagcga ctgcgtcgac gtcgccgagg
     2521 caatgatcta caacgagtgg gcgcagctgc tcgagaccac cgacccgaat accggcgagc
     2581 tgttccagcc ggaccagttc gaagtgttcg acttcaacga gctcggcacg gccatgctgc
     2641 aggacgggct gtacgcccgg cagtcgtggc tggacgaggg gaacaacacc gaagtcgcga
     2701 tgcggttcct ccaggcctcg ttccggggct ggatgtactg ccgggacaat cccgatgact
     2761 gcgtccagta ctccatcgat cggggcagca tctgggaagc gggccacctg gcctggatga
     2821 tgaacgagat caacaagctg gtctggccgg ccgaggccgg catcggccac ctggatgaag
     2881 gagagctcac ccagaccgtg gataccgcga gcgcggcggg gatcctgacc ggaccaccgg
     2941 acgacgcggc gttcaacagc gctatctggg aagaggcgct ttcggggatc gacggtgata
     3001 cgaccggcga tgactttgcc gcgcctgagg tcccgatcac gcccggcggc gagtaaccga
     3061 cgagtgggat gaagccccac aatctgaagc cgatggaggc ccctcgtcc
//
LOCUS       DASYGU010000091        10496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_529509, whole genome shotgun sequence.
ACCESSION   DASYGU010000091 DASYGU010000000
VERSION     DASYGU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529509"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..612
                     /locus_tag="VGO32_02370"
     CDS             31..612
                     /locus_tag="VGO32_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV7199630.1"
                     /translation="MTAVAQTPPSPSPWAITTRVIVYAAIGAALYAIFNWIAFAIQIP
                     GTENVSVRPHYGLLTFFGFSFGPVVGFLTGFVGNTVGDQLAYGSAFGSWWWSVANGLA
                     GLVAGLFPFVLASRMTSVGSKAAWSAVAGVVATIVGFLFIWIELIIQPELGFNTILTA
                     EYIPVIVGNSIAAAIITPILVLAWEPISEQLGR"
     gene            640..1479
                     /locus_tag="VGO32_02375"
     CDS             640..1479
                     /locus_tag="VGO32_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEV7199631.1"
                     /translation="MRAAFRYLGRGSFLARRDPRVLILVAVLFVFGASQLRDIRQLLL
                     VFALALGYYSLASIPWALVRVQWIYLLVVVSMFAAFNAILTGGQAGSFEGTDPHVLVE
                     LPFGLVLTAEGVSLALTQVVRYLSFAAVGFPVAYAIAPGDFGVALRRLGLGDRFSVMI
                     DLTVRFVPTLASELSETIDAQRVRGYEPLARRGGLRERVRRLGPLFVPATVGALVGAE
                     DTIDAMDLRGFATGKRTWYRELHMDGASWLILGAFVAFLVVATFLNASGGSEHYLLPI
                     FVP"
     gene            1745..2191
                     /locus_tag="VGO32_02380"
     CDS             1745..2191
                     /locus_tag="VGO32_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7199632.1"
                     /translation="MTTIDPSGPPPAPPTVNGVSHLPPHVQRALARPHRIDITTTGRQ
                     SGQPRRIELVFHVIDGRIIISGMPGRRDWYANLVADPVMTFHLTGPVQADLPSLARPI
                     TEPTERRQIMEAVTRNWNAEDRFDTFYRHSPLIEVTFPATPVAGAA"
     gene            complement(2221..3516)
                     /locus_tag="VGO32_02385"
     CDS             complement(2221..3516)
                     /locus_tag="VGO32_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV7199633.1"
                     /translation="MGRIALYSAAVTAVILLSLIWVAVAGGSSPTGTGASPTPSSAGG
                     AGILGEITISAFDLGFEPALVDVPKPGTYTVHFMNDGAIDHDLTFAGQPTQAAGPGEM
                     VMFEVTIPEGGTTFQCTVPGHADGGMTGTVTVTGVGGGGHEGMTAEEMRDVDAAVTAR
                     FPEETTAHGGQRLEPEILADGTKVFELTASVIQWEVEAGHFVEAWAYNEMVPGPEIRV
                     QLGDRIQIVLHNELEVPTTLHSHGLIVPNAMDGVPAITQDAILPGESFTYEFTVRNAG
                     SHMYHSHFMAQRQVPMGLLGAFVVEDPSEPVVDLDLPMILNDGPLGFTIDGKSFPATA
                     PIVATQGQLIRIRYMNEGLQIHPMHLHGIPQQVIARDGFMLEQPYFEDTVLVAPGQRV
                     DVLIRASELGVWAFHCHILSHAESDEGMFGMVTALIVNP"
     gene            complement(3697..4101)
                     /locus_tag="VGO32_02390"
     CDS             complement(3697..4101)
                     /locus_tag="VGO32_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HEV7199634.1"
                     /translation="MSVKWILRIESAAIGIVSVAGYLIIGGPPLLLIPALLAPDLSAL
                     GYLAGPRAGAVTYNLVHNWALPAALALLAILGHVLWPMAVAAILAAHVGFDRALGYGL
                     KLPTSFQDTHLGRIGRDRSQVDSGAGPGSLSD"
     gene            complement(4118..5212)
                     /locus_tag="VGO32_02395"
     CDS             complement(4118..5212)
                     /locus_tag="VGO32_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199635.1"
                     /translation="MAGRFEIFWHLFIASGKMFLRDRTAVFFSFFLPLVIMLIFGVLN
                     FGAGEALNLGVVDEADTESSRAVIAAFEEVPILEVIPGDLDEELAETSEGNRALVVVF
                     PAGWELVPPGSPTYQPLTAYMSEATPGEGQTALLVVNAVVAQGIVTAGGGSPEPLVVV
                     EEVAGRNLGYIDFLVPGILGLTIMQLGIFSVAFGFVQLKTTGTLRRLFATPTSPSYFL
                     SAQVGSRLVIALIQVLILLGVGIWFGVQLVGSVFVVLALSIIGSVVFLAMGFGIAGWA
                     KNENQAAPVANLISLPMMFLSGSFFPREAMPEFLQQVTRFLPLTYLNDALRAVINDGA
                     GLDVIGTDIIGLAVWGVISFVIATRLFKWE"
     gene            complement(5216..5983)
                     /locus_tag="VGO32_02400"
     CDS             complement(5216..5983)
                     /locus_tag="VGO32_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199636.1"
                     /translation="MTSPQPIIVARDLRKSYGALTAVDGVSFEVAEGEVFGILGPNGA
                     GKTTTLEMLEGMRPIDGGSATVDGIDVTADPRGVKARIGIQLQASAFFEELNLTELLV
                     LFGHLYHVRADAAALLAGVELTDKAKSQVKTLSGGQKQRFSIAAALVNDPRVLFLDEP
                     TTGLDPQARRHLWGVISDIRRRGHTVILTTHYMEEAETLCDRIAIMDGGKIVALDTPD
                     AMIDSLIGRGFTKERTERQANLEDVFIELTGHDLRED"
     gene            complement(5980..7176)
                     /gene="glgA"
                     /locus_tag="VGO32_02405"
     CDS             complement(5980..7176)
                     /gene="glgA"
                     /locus_tag="VGO32_02405"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEV7199637.1"
                     /translation="MRVLILTNEFPPSVYGGAGVHVGELTNALRGRVELDIRTFGDHA
                     HDEPGWRVHGYPAALDVSRAPDELRPVLSAFGRNLAMAADAPTADLVHCHTWYTHLGG
                     LVIGQAYGLPLVVTVHSLEPLRPWKREQLGGGYDASAWVEREALEGADGVIAVSDATR
                     RDVLDHFDVDPARIHVVHNGIDPVDFAPDPNRDTLRRFGIDPAAPYVLFVGRVTRQKG
                     ITTLVDAIPHLDPRIAVVLAAGQPDTPEIAREVEAGLAEARAVAPTRSIHWIPEMLDR
                     RTVRQMYSHAAVFVCPSAYEPFGLTNLEAMACGVPVVATRVGGIPEIVDHGRTGLLID
                     VPDEPATDGGADVALALAAAINDLVADPARRAAMGEAGRERAVEQFSWAGVANRTIAV
                     YESVVR"
     gene            7281..9596
                     /locus_tag="VGO32_02410"
     CDS             7281..9596
                     /locus_tag="VGO32_02410"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV7199638.1"
                     /translation="MTTSLPSAPLVEIVLPVEGMSCATCVNRIERFLRRADGVETATV
                     NLATERAAVRYDPARIDRAGIVGAIEAAGYDVRPDVRNQPAGVPSETDAAAEAATDTA
                     RAAERSALLRDGVAATAVGLAMMVATLWPGGLPLTMAQLNLVLLVPAMFVQLVLGRRF
                     TSRALRGLAHGELTMDTLVAMGTWAAFLYSAAIAVAPGPATAAGLPLDTFFETASIII
                     GLVLIGRWLEARARSEASGAVRRLLRRRPTTAHVIRDGTEEEVALDAVRVGDLVRVRP
                     GERIAVDGAVVEGASAVDESLLTGEAVPVDKAAGDRVTGATLNAQGVLVVRTERIGAD
                     TTLAQIARLVEQAQGSKAPIQHAVDRVVSWFVPAVAIVAALTFLTWMVFGPEPRLPVA
                     LASAIGVLIIACPCAMGLATPTALIVGAGRGADAGILLRDAAAVERAATITDVVLDKT
                     GTLTRGRAEVSVVLATKPGPDARHALLRDAAAAERGSEHPFASAIVRQAGAEGIDVEP
                     MVVTGFTAVAGRGVTADVEGRTVLIGTARFLAEAGIASATAHVLADAATNAEAEGRTA
                     VWVGRDGAVAGLIILADRLKPSATPAIARLERSGLRPWLLTGDRAAAARAVASELGLP
                     ADRVRAEVRPEGKAAAVAALQAEGRRVAMVGDGLNDAPALAQADLGIAIGTGADVAIE
                     ASDMTLVGDDLRGVATGIALARATMRIIRQNLAWAFGYNIILIPVAAGILYPFTGWTL
                     NPALAAGAMALSSVSVVANSLRLRRVRLVTD"
     gene            9611..9805
                     /locus_tag="VGO32_02415"
     CDS             9611..9805
                     /locus_tag="VGO32_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEV7199639.1"
                     /translation="MQTTTDTEPRDPVCGMSVDPVRAEANGLVAEHNGVTYLFCGRGC
                     LLDFRDDPDHYLDPGYVPHM"
     gene            9817..>10496
                     /locus_tag="VGO32_02420"
     CDS             9817..>10496
                     /locus_tag="VGO32_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7199640.1"
                     /translation="MGAERPVGPPWGHEWVGRFHEHVFDSAVLAGNPLSDPARRPLYV
                     YTPPSYEAEPDRRFPTVVVIQGLTGQVDMWRNRKAFSRNLPETVDMALAAGGQAPAVV
                     VFVDCWTSLGGSQFLDSPGTGNYLTYLCDEVVPWVDATYRTLPEAAHRAVAGKSSGGY
                     GAMVLPMLRPDRFGALATHAGDALFEACYLPDIRQAARALRDRYGGSYEQFWATFRSR
                     VGPIPNSD"
BASE COUNT         1581 a         3593 c         3630 g         1692 t
ORIGIN      
        1 cgaactgacc ccgaggagga gcattcgccc atgaccgctg tcgcacagac cccgccaagc
       61 ccgagtccct gggccatcac gacccgggtt atcgtgtacg ccgccatcgg cgcggcgctg
      121 tacgccatct tcaactggat cgccttcgcg atccagatcc cggggaccga gaacgtcagc
      181 gtgcggcccc actatggcct cttgaccttc ttcgggttct cgtttggccc ggtcgtcgga
      241 ttcctgacgg ggttcgtcgg gaacacggtt ggagaccagc tggcctatgg cagcgcgttt
      301 ggctcgtggt ggtggagcgt cgctaacggt ctggcgggcc tggtggcggg gctctttccg
      361 ttcgtactcg cgagccgcat gacaagcgtg ggcagcaagg cggcatggtc agcagtcgcc
      421 ggagtagtcg ctaccatcgt ggggttcttg ttcatctgga tcgagctgat cattcagcct
      481 gagctcgggt tcaacacgat cttgaccgcg gagtacatcc cagtcatcgt cgggaattcg
      541 atcgccgccg cgatcattac tccgatcctc gtcctcgcct gggagccgat ctcggaacaa
      601 ctcggccgct gatttcgtcg ggacgcggcc tggtggacca tgcgggccgc gttccgctat
      661 ctcggtcggg ggtcgtttct ggcccgccgg gacccccgcg ttctcatcct cgtcgcggtc
      721 ctgttcgtct tcggtgcaag ccaactacga gacatccggc agctcctcct cgtgttcgcc
      781 ctcgcgctcg gttactactc gctcgcttcc atcccgtggg cattggtacg agttcaatgg
      841 atctacttgc tcgtcgttgt ctcaatgttc gctgcgttca acgcgatcct gacaggcggt
      901 caggccggtt cgttcgaggg caccgacccc cacgtcctgg tcgagctccc attcggattg
      961 gtacttaccg ctgagggggt aagcctcgcg ctcactcagg tggttcgcta cctatctttc
     1021 gcggcggtcg ggttcccggt ggcatatgcg attgcgccag gcgatttcgg tgtcgccctc
     1081 cgtcgacttg gcctcggtga ccgcttctcg gtgatgatcg acctaactgt gaggttcgtc
     1141 ccgaccttgg ccagcgagct tagtgagacg atcgacgccc agcgcgttcg tggctacgag
     1201 ccgctagcgc ggcgcggcgg tcttcgggag cgggtgcgtc ggcttggacc gctcttcgtc
     1261 ccggcaacgg tcggtgcgct ggttggtgcc gaggacacca tcgacgcgat ggatctccgc
     1321 ggcttcgcga cggggaagcg cacctggtat cgcgaactcc atatggatgg cgcgtcatgg
     1381 ctgatcctcg gcgccttcgt cgctttcctc gtcgttgcaa cgttcctcaa tgcgagcggt
     1441 ggttccgagc actacctgct ccctatcttc gtgccctgac cgccggtctc ggcacgacgc
     1501 gggcggccgc cacgctcacg gtcgtggaca gggcgtggat aagtcggtca ccaaccggtc
     1561 aaactcgtgg ataagccgct tggcgaaccg cgaacggtcc cgtattgttc tggttgtccc
     1621 ggaggagccg gacgagtcgg gagattgcat caagcggccc gccaggttcc tccggggcgt
     1681 ttttgcgccc ggaagcattt cggcgtacag tctcgctctg aatctcatag cgagtcccgt
     1741 cccgatgacc accattgatc cgtccggtcc cccgcccgcg ccgccgaccg tgaacggtgt
     1801 ctcccatctc cccccgcacg tgcagcgcgc gcttgcccgg ccgcaccgga tcgacatcac
     1861 gaccaccggc cggcagagtg gacagccacg ccggatcgag ctcgtgttcc acgtcatcga
     1921 cggccggatc atcatcagcg gcatgcccgg tcgacgcgac tggtacgcca acctggtcgc
     1981 cgacccggtg atgaccttcc atctgaccgg tccggttcag gccgacctgc cgtccctcgc
     2041 ccgcccgatc acggagccca ccgagcggcg ccagatcatg gaggcggtca cgcgcaactg
     2101 gaacgccgag gaccggttcg acacgttcta ccggcattcg cccctgatcg aggtcacctt
     2161 tcccgcaacg ccggtcgccg gcgcggcctg acccccggag ataactccag gctctgtgag
     2221 ctacgggttg acgatcagcg cagtcaccat cccgaacatc ccttcgtcgc tctcggcgtg
     2281 ggacaggatg tggcaatgga atgcccagac gccgagctcg ctggcgcgga tcagaacatc
     2341 cacgcgctga ccgggagcca cgaggaccgt atcctcgaag tacggctgct cgagcatgaa
     2401 tccgtcccgc gcgatgacct gctgcggaat cccgtgcagg tgcatcggat ggatctggag
     2461 cccctcgttc atgtaccgga tgcggatcag ctgaccctgg gtcgcgacga tgggcgcggt
     2521 ggccgggaag ctcttgccgt cgatcgtgaa tccgagcggc ccgtcgttga ggatcatcgg
     2581 aaggtcgagg tccacgactg gctccgacgg gtcctcgacc acgaacgcac ccaggaggcc
     2641 catgggcacc tggcgctggg ccatgaagtg gctgtggtac atgtgtgaac ccgcgttccg
     2701 gacggtgaat tcgtaggtga agctctcgcc cgggagaatg gcgtcctggg tgatggcggg
     2761 cacgccgtcc atggcgttgg gaacgatcag cccgtgtgag tggagggtgg tcggcacctc
     2821 gagctcgttg tggagcacga tctggatgcg gtcaccgagc tggacccgga tctccggccc
     2881 gggcaccatc tcgttgtatg cccaggcctc gacgaagtgg ccggcctcga cctcccactg
     2941 gatgaccgac gcggtcagct cgaagacctt cgtgccgtcg gcgaggattt cgggctcgag
     3001 ccgctggcca ccgtgtgcgg tcgtctcctc cgggaaccgg gcggtgacgg ccgcgtcgac
     3061 gtcgcgcatt tcctcggcgg tcatgccttc gtgcccccca ccgccgacgc cggtcacggt
     3121 gaccgtgccg gtcatgccgc cgtcggcgtg gcccggaacc gtgcactgga acgtggtgcc
     3181 gccctcggga atggtcacct cgaacatgac catctcgccg gggcctgccg cctgcgtggg
     3241 ctggcccgcg aacgtgaggt cgtggtcaat ggccccgtcg ttcatgaagt ggacggtgta
     3301 cgtgcccggc ttcggcacgt cgaccagcgc gggttcgaag ccaaggtcaa aggcgctgat
     3361 cgtgatctcg ccgaggattc cggcaccgcc cgcgctggat ggcgtggggc tcgccccggt
     3421 ccccgtggga ctggagccgc cggccacggc gacccagatc aacgacagca ggatgacggc
     3481 ggtcacggcg gcgctataga gagcgatacg gcccatcggt gggggctcct tctgatctgt
     3541 tgcggagtgg atcggctccc cacagactga ccgacgaacg ccgagcggag tcaggtgcca
     3601 acggcccgaa atcgatgggc cgttcggccg gtggcgggac agccggtcag cgtgcagctg
     3661 ccgggtcgcg tggcgcgcgc accgcgccga tggcgctcag tcggagagcg accctggccc
     3721 ggcaccggag tcgacctgcg accgatcgcg accgatgcgt ccgagatggg tgtcctggaa
     3781 gctggtcggc agcttcagtc catagcccag cgcgcggtcg aagccgacgt gcgcggcgag
     3841 aatggcggcc accgccatcg gccacaggac gtggcccagg atcgcgagca gggcgagcgc
     3901 ggccggcagt gcccagttgt gaaccaggtt gtaggtgacc gcccccgcgc gcgggccggc
     3961 caggtagccg agcgccgaca ggtccggcgc gagcagggcg ggtatcagga gcagcggcgg
     4021 gccgccgatg atcaggtacc cggccacgct cacgatcccg atcgcggccg actcgatgcg
     4081 gaggatccac ttcacgctca cgggcgggga ccctgcctca ctcccacttg aacagccggg
     4141 tcgcgatcac gaacgagatg accccccaca ccgcgagccc gatgatgtcg gtgccgatca
     4201 cgtcgagacc ggcgccatcg ttgatgaccg ccctcagcgc gtcgttgagg taggtcagcg
     4261 gcaggaatcg ggtcacctgc tggaggaact cgggcatggc ctcgcgcggg aagaacgagc
     4321 ccgacaggaa catcatcggc aggctgatca ggttggcaac cggggccgcc tggttctcgt
     4381 tcttggccca gccggcaatc ccgaagccca tggccaggaa gacgaccgaa ccgatgatgg
     4441 acagggcgag caccacgaag acgctgccta ccagctggac cccgaaccag atccccaccc
     4501 cgagcaggat gaggacctgg atcaaggcga tgaccagtcg cgagccgacc tgggcggaga
     4561 ggaagtagct gggggacgtc ggcgtcgcga acaggcgacg aagggtgccg gtcgtcttca
     4621 gctgcacgaa cccgaaggcc acgctgaata tgccgagctg catgatcgtc aggcccagaa
     4681 tgccggggac caggaagtcg atgtagccga ggttgcggcc ggccacctcc tcgacgacga
     4741 cgagtggctc cgggcttccg ccgcccgcgg tcacgatgcc ctgcgcgacg accgcgttca
     4801 cgacaaggag tgcggtctgc ccttcgccgg gggtggcctc actcatgtac gccgtgagtg
     4861 gctggtaggt cggggagccg ggcgggacca gctcccagcc tgcggggaag acgaccacga
     4921 gcgcgcgatt gccctcgctg gtctcggcca gctcctcgtc caggtcgccg gggatgacct
     4981 caaggatggg cacctcctcg aacgcggcga tgaccgcccg gctcgattcg gtgtcggcct
     5041 cgtcgacgac gccgaggttc agcgcctccc cggccccgaa gttcaggacc ccgaagatca
     5101 gcatgatcac gagcggcagg aagaagctga agaacaccgc ggtccggtcg cgcaggaaca
     5161 tctttccgga cgcgatgaac aggtgccaga agatctcgaa ccggccggcc atcggtcagt
     5221 cctcgcgcag gtcgtggccg gtgagttcga tgaacacgtc ctcgaggttt gcctgccgct
     5281 ccgtgcgctc cttcgtgaat ccgcgaccga tcagggagtc gatcatcgcg tcgggcgtgt
     5341 cgagcgccac gatcttcccg ccgtccatga tcgcgatccg gtcgcagagg gtctcggcct
     5401 cttccatgta atgggtggtc aggatcacgg tgtgcccgcg ccgccggatg tcgctgatga
     5461 ccccccacag atggcgccgg gcctgggggt cgagcccggt cgtcggctcg tcgaggaaca
     5521 gcacccgggg gtcgttgacc agcgccgcgg cgatgctgaa gcgctgcttc tgcccgcccg
     5581 acagggtctt cacctggctc ttcgccttgt cggtgagctc gacgccggcg agcagcgcgg
     5641 ccgcgtcggc ccgcacgtgg tagaggtggc cgaacaggac aagcagctcg gtgaggttca
     5701 gctcttcgaa gaacgccgac gcctggagct ggatcccgat ccgggccttg accccgcgcg
     5761 ggtcggccgt gacatcgatc ccgtccacgg tcgccgaccc gccgtcgatc ggccgcatgc
     5821 cctccagcat ctcgagggtc gtggtcttgc cggccccatt cgggccgagg atgccgaaca
     5881 cctcaccctc ggccacctcg aagctcacgc cgtcgaccgc ggtgagtgca ccgtacgact
     5941 tgcgcaggtc gcgggcgacg atgatcggct gcgggctggt catcgcacga ccgactcgta
     6001 gacggcgatc gttcggttcg ccacgccggc ccagctgaac tgctcgacgg cgcgttcccg
     6061 accggcctcg cccatcgccg cccgtcgggc cgggtccgcg acgagatcgt tgatcgcggc
     6121 cgccagcgcg agcgcgacgt cagcgcctcc atcggttgcc ggttcgtcgg gcacgtcgat
     6181 gagcagcccg gttcggccat ggtcgacgat ctccgggatg cccccgaccc gggtggcgac
     6241 gaccggcacg ccgcaggcca tggcctcgag gttcgtgagg ccgaacggct catatgccga
     6301 tgggcacacg aagacggcgg cgtggctgta catctggcgg accgtgcgtc ggtcgagcat
     6361 ctcggggatc cagtggatgg accgtgtcgg cgcgaccgcg cgggcctcgg ccagcccggc
     6421 ctcgacctcc ctcgcgatct ccggggtgtc cggttgcccg gcggcgagaa cgaccgcaat
     6481 tcgcggatcg agatgcggga tcgcgtccac cagggtggtg atccccttct ggcgggtcac
     6541 ccggccgacg aacaggacgt agggcgccgc ggggtcgatc ccgaatcggc ggagtgtgtc
     6601 gcggttcggg tccggggcga agtcaaccgg gtcgatcccg ttgtggacga cgtggatgcg
     6661 ggccgggtcg acgtcgaaat gatcgaggac atcgcggcgg gtcgcatcac tgaccgcgat
     6721 cacgccatcg gccccctcga gcgcctcgcg ctcgacccag gccgacgcgt cgtagccgcc
     6781 gcccagctgc tcgcgcttcc acggccggag tggctcgagc gaatggaccg tgaccaccaa
     6841 cggcaggccg taggcctgac caatgaccag gccgccgagg tgggtgtacc aggtgtggca
     6901 gtggacgagg tcggcggtcg gcgcgtccgc cgccatggcg aggttgcggc cgaacgccga
     6961 caggaccggc cggagctcgt ccggagcgcg ggagacatcg agcgcggcgg ggtacccatg
     7021 cacccgccag ccgggttcgt cgtgggcatg gtcgccaaag gtgcggatat cgagctcgac
     7081 ccgtccacgc agcgcgttcg tcagctcgcc gacatggacc ccggcgccgc cgtagaccga
     7141 cggggggaac tcgttggtca ggatcaggac acgcaccaga ccagcctacc gtggttgtcc
     7201 gggcatcggc cgcctcgggg ccgcgttgac gctcgcccga tactccccca tagtatccgc
     7261 acgagccctt cccgagtcca atgaccacct ctctgccctc agctcccctg gtcgagatcg
     7321 tcctccccgt cgagggcatg agctgcgcga cctgcgtgaa ccgcatcgag cggttccttc
     7381 gtcgggcgga tggagtggag acggccaccg tcaacctcgc caccgaacgc gccgcggtgc
     7441 gatacgaccc ggcgcgcatc gaccgggccg ggatcgtggg cgccatcgag gccgccggct
     7501 acgacgtgcg gcccgatgtc cggaaccaac cggcgggtgt cccttcggag actgacgcag
     7561 cagcagaagc ggcaaccgac acggcgcgcg ccgcggaacg gtcggccctc ctccgcgacg
     7621 gggtggctgc caccgccgtc ggtctggcga tgatggtcgc gaccctgtgg cccggcggac
     7681 tgcccctgac catggcgcag ctcaacctcg tcctgctcgt cccggccatg ttcgtgcaac
     7741 tggtcctggg tcgtcggttc acgtcccgcg cgctccgcgg actggctcat ggggagctga
     7801 ccatggacac ccttgtcgcc atggggacct gggcggcgtt cctgtactcg gcggccatcg
     7861 ccgtggcccc tggccctgcg accgccgccg gtctgccgct cgacacgttc ttcgagaccg
     7921 cgtcgatcat catcgggctc gtgctcatcg gccgatggct ggaggcacga gcccggagcg
     7981 aggcctcggg cgcggtccgc cgactcctgc gtcggcgtcc gacaaccgcc cacgtcattc
     8041 gggacggcac cgaggaggag gtcgcgctgg acgccgtccg ggtgggcgac ctcgtccggg
     8101 tccggcccgg tgagcggatt gcggtcgacg gggcggtggt cgagggcgcg agcgcggtgg
     8161 atgaatcgct gctcaccggt gaggcggtcc cggtggacaa ggcggcaggc gaccgggtca
     8221 cgggcgcgac cctcaacgcc cagggcgtgc tcgtggtccg cacagaacgg atcggcgccg
     8281 atacgaccct cgcccagatc gctcggctgg tcgagcaggc acagggctcg aaggccccga
     8341 tccagcatgc ggtggaccgg gtggtcagct ggttcgtgcc ggcggttgcc atcgtggccg
     8401 ccctgacctt cctgacctgg atggtgttcg ggccggagcc gcgcctgccg gtggcactgg
     8461 ccagcgcgat cggtgtcctg atcatcgcct gcccatgcgc catgggcctc gctacgccga
     8521 ccgccctgat cgtgggcgcc ggtcgaggcg cggatgccgg catcctcctc cgcgatgcag
     8581 cggcggtcga gcgggcggcc accatcaccg acgtggtgct ggacaagacg ggcacgctga
     8641 cgcgcggtcg ggccgaggtc agcgtggtgc tggccaccaa acccggcccg gacgcccgcc
     8701 acgcgctgct ccgcgacgcg gcggcggcgg agcgcgggag cgagcatccg ttcgcctcgg
     8761 ccatcgttcg gcaggccggg gccgagggaa tcgacgtcga gccaatggtg gtgaccggct
     8821 tcacggccgt tgccggccgc ggggtgacgg cggacgtgga gggccgcact gtcctgatcg
     8881 gaacggcccg attcctggcc gaggcgggaa tcgcgtcggc gacggcccac gtcctggcgg
     8941 atgccgcgac caacgccgag gcggagggcc gtaccgcggt ctgggtcggg cgggatgggg
     9001 ccgtggccgg gctcatcatc ctggctgacc ggctgaagcc gtcggccacg ccggcgatcg
     9061 cccggcttga gcgatctggt ctccggccat ggctcctgac cggagaccgc gcggcggccg
     9121 ccagagccgt ggcgtccgag ctcgggctcc ccgccgaccg ggtgcgcgcc gaggtccggc
     9181 ccgagggcaa ggccgccgcg gtggccgcgc tccaggcgga gggccggcgg gtggcgatgg
     9241 ttggcgacgg cctgaacgat gcgccggccc tggcccaggc cgatctcgga atcgcgatcg
     9301 gcaccggcgc cgatgtcgca atcgaggcca gcgacatgac cctcgtcggc gacgacctgc
     9361 gcggggtggc gaccggcatc gccctggcgc gagccacgat gcggatcatc cgccagaacc
     9421 tggcgtgggc cttcggctac aacatcatcc tgatcccggt cgcggcgggg atcctgtacc
     9481 cgttcaccgg atggaccctg aacccggcgc tggccgccgg agccatggcc ctgtcgagcg
     9541 tgagcgtggt ggcaaactcg ctccggctgc gacgcgtgcg gctcgtcacc gactgaccca
     9601 aggaggaacg atgcagacga ccaccgatac cgagccacgg gacccggtct gcgggatgtc
     9661 cgtcgacccg gtgcgcgccg aggccaatgg cctcgtggcc gagcacaacg gcgtgaccta
     9721 cctgttctgt ggccgcggtt gcctgctcga tttccgcgac gaccccgatc actacctcga
     9781 ccccggctac gtcccgcaca tgtaggctgc gacccgatgg gcgcggaacg accagtgggg
     9841 ccaccgtggg gccacgagtg ggtgggccgg ttccacgagc acgtcttcga ctcggcggtg
     9901 ctggccggca accccctgag cgatccggcc cgccgaccgc tgtacgttta caccccgccg
     9961 tcctacgagg ccgagccgga ccgtcgattc ccgaccgtcg tcgtcatcca gggcctcacc
    10021 ggccaggtcg acatgtggcg caaccgaaaa gccttcagcc ggaacctgcc cgagacggtg
    10081 gacatggcgc tcgccgccgg gggccaggcg ccagcagtgg tcgtcttcgt ggactgctgg
    10141 accagcctcg gcggcagcca gttcctcgac tcgcccggaa ccgggaacta cctcacatac
    10201 ctgtgcgacg aggtcgtccc atgggtggac gcgacgtacc ggaccctgcc cgaggccgcg
    10261 caccgggccg tggccgggaa atcgtcaggc ggatacgggg ccatggtcct acccatgctc
    10321 cgcccggacc gattcggtgc gctggcgacc cacgccggcg acgcgctgtt cgaggcgtgc
    10381 tacctgcccg acatccggca ggcggcgcgg gcgcttcggg atcggtatgg cggctcgtac
    10441 gagcagttct gggcgacgtt ccggtcgcgc gtcggcccga tcccgaactc cgattt
//
LOCUS       DASYGU010000092         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_427580, whole genome shotgun sequence.
ACCESSION   DASYGU010000092 DASYGU010000000
VERSION     DASYGU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427580"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(781..868)
                     /locus_tag="VGO32_02425"
     tRNA            complement(781..868)
                     /locus_tag="VGO32_02425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Ser,seq:tga)
     gene            complement(918..1448)
                     /locus_tag="VGO32_02430"
     CDS             complement(918..1448)
                     /locus_tag="VGO32_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEV7199641.1"
                     /translation="MDIFIHSEGNARYVAAVVANLGDTYGQRISVCMRADRIPGYKLV
                     FMLWPANGSGNSRGEIDYPEGQLTPEGVGNAFMHYDPKPASNPKQDAYSTGMSTVDWH
                     VYTMEWNPGSSAQSDDYASFYVDGRLIGRSTGASVPDGPMHYLIQTETYVAGQALPPP
                     ASGHILFDWITIATPN"
BASE COUNT          302 a          719 c          847 g          372 t
ORIGIN      
        1 tacgttccgc ggccactcga gtcgcttgcg ccggcctggc gggcgaacca gtcgcgggca
       61 gcagcgggcc ctggccacgc cccgagggcg gtggtctcat cgaaggtcga cgtcaccctc
      121 cgtgtccagc cggccggctc gctcggaacc gcggcgcagc tcgcgctcat tgcggtcggg
      181 ggcggtggct gcggcgggtc ggcgacagcg atcgggggtt ccagggaggg cgtcacgggt
      241 tcggtcgggg ccggcgactc gtcggcggtg tcgatatcgg ctctagtcgg ccaatcgtgg
      301 gaggtcggct ccggcgcgga agacgtggtt gattcgccca gccatggccc gattggcgtg
      361 cgtaccgaag cgctgcctcg cgacggagcg tgtgacggcg aaccatccat cgcgataccg
      421 ggtggtgccg gtgcgggcgc gggaaggagg ctgtccggtc ggccgagggt ggtcccgagg
      481 ggtgcatcgt ccgcagccgc gtacgtcgcc gccgcgacgg ccacgagggt acccgccagt
      541 acggcgttga gcaggcgtcg aggtcggatg tcctgcattg gcggcgttcc ttcgcggatg
      601 gcccggtcgt ggggaacggc tgggcagtag cttcggtgtt gtggcgggct ggccatctgg
      661 gcagtccgag ggaacgcgaa gggaccctcg cacggtgggc aggaccgcgg agtgcgggga
      721 catggtaccc ggactgcgcc ccggctcggg ccagatctgg gcgcagccgg gacctccgcg
      781 tggcggaggg ggtgggattc gaacccacgg aggcttgcgc ctcaacggtt ttcaagaccg
      841 ccgcattcgt ccgctctgcc acccctccga gttcgattat cggcggccgc ggaggccgag
      901 gctagttcgg cacaacgcta gttcggggtg gcgatcgtga tccagtcgaa caggatgtga
      961 ccagatgccg gcggtggcag ggcctggccg gcgacgtatg tctcggtctg gatcaggtag
     1021 tgcatcggcc cgtccgggac gctcgcgccc gtcgaacggc cgatgagccg accgtcgacg
     1081 tagaacgatg cgtagtcgtc cgactgggct gaactccccg gattccattc catggtgtac
     1141 acgtgccagt ccacggtgga catgcccgtg ctgtacgcat cctgcttcgg attcgacgcc
     1201 ggcttggggt cgtagtgcat gaacgcgttg cccacgccct cgggcgtcag ctggccctcg
     1261 gggtagtcga tctcgccccg cgagttcccg ctcccgttgg ccggccacag catgaacacg
     1321 agcttgtagc ccgggatccg gtcggcccgc atgcacacgc tgatgcgctg gccgtaggtg
     1381 tcccccaggt tggcgacgac cgccgcgacg tagcgagcat tcccttcgga gtggatgaag
     1441 atgtccatga cgccgttgtg ttcactgacg gtcttaccac tgttgtacgt cccccgaccg
     1501 ctggagtcgg taccgccctg acggttccgc caggccgcgg cggcggccgg acccggccag
     1561 ctcccgatcg gcgtcgtttc gctgaagttg gatgtcacct gccgggtcca gcccgccggc
     1621 tcgcccggga tcgcggcgca gctggcgctg aagctcgtgg gcgggggagg cggcggtgcc
     1681 ggagggggcg gcggaggcgg cggtggggcg gccgccgggg gtggggcggc cgtcggcggt
     1741 ggggtcgggg cctgtgctgg accggtcgtt gtcccaggcg gtgtcgggcc gggggcctcg
     1801 ctggggtcag ccgtctccat cggtcccgaa ccaatggcgc tgggcatcct ggacggcgat
     1861 gctgatggcg gcgtggacga gctgccgtgc ggctccattg gcgaacccgg ttccgccgag
     1921 gcgaattccc ggacggctac ctgattctcg ccgaccgccg cgaacgtgaa ggtcgcgagc
     1981 acctcggcca tgagggctcc cgccagcgcg atgatgagca gtcgacgccg tccaacttcg
     2041 cgcatgggtt cggcctcccc gcggttcgca gtccccgcca ccaaaaccag gaccctggca
     2101 tccatgaacg tggatgccga ctggcgagcc aagcgctctc ggtggctgtg ccgagcctag
     2161 gagcgaccgt gcgcgaccga cataggccag gcgtactgat ccgaatggac tgccttgatg
     2221 ctggtcggag agcatccggc
//
LOCUS       DASYGU010000093         9092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1507346, whole genome shotgun sequence.
ACCESSION   DASYGU010000093 DASYGU010000000
VERSION     DASYGU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507346"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGO32_02435"
     CDS             complement(<1..396)
                     /locus_tag="VGO32_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199642.1"
                     /translation="MSPRLRRIVAVVTLLLALVLVAVLTLRALGVPIALGPGTATPMP
                     TPIASAEPSSGATPSLDEALAAIEADTADIRDLPAADIGPPEFVTRDELERRLAERFA
                     EDYPAEQQAVDNALYHALGILAPDQDYAAL"
     gene            601..1242
                     /locus_tag="VGO32_02440"
     CDS             601..1242
                     /locus_tag="VGO32_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7199643.1"
                     /translation="MEITWYGRACFRLKGKRAAVITDPCPPKTGFVAGKHDVDLLTIS
                     HDHPDHTYTRSISAGLTLTRPGEYEFADLLVTGVRTWHDGVEGKERGENVIFTFEVDG
                     VHVCHLGDLGHLLTDEQLHEIGPIDVLLVPAGGQFTISPAEAAEVVSQLEPKVVIPMH
                     YALDGGSKDLLGPETFLHELATEPIRLPKASLTPSTLPEETQVVLLEARGRTA"
     gene            complement(1239..2447)
                     /locus_tag="VGO32_02445"
     CDS             complement(1239..2447)
                     /locus_tag="VGO32_02445"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEV7199644.1"
                     /translation="MTSVRSRNPLAFLDADLDDLRAKGLYRRLRVVESEQAARCRIDG
                     REVITLSSNNYLGLTTHPKLRAAMIAATEELGAGSGAVRTIAGTMTIHQQLEARLAEF
                     KQVEATLTFQSGFTANTGTVPILAGEGAVIVSDELNHASIIDGIRLTKAERKVFPHAD
                     TDALRVILREIRAADGGAGRTILVITDGVFSMDGDIAPLPGIVEAAEEMEAIVYVDDA
                     HSSGVLGRNGRGTVDHFDLHGRVHVQVGTLSKAIGVVGGYVAGALSLREVLIHRARPF
                     LFSTSHPPGVAAACLAAIDVLEQEPERIERLWANARRFKDGLGRLGFDTGASETPITP
                     VIAGGGALAMKLSDRLFEEGVFAQGIGFPTVPEAKSRVRTIVTAEHTDEDLDQCIAAF
                     EKVGRELALI"
     gene            complement(2444..3469)
                     /gene="tdh"
                     /locus_tag="VGO32_02450"
     CDS             complement(2444..3469)
                     /gene="tdh"
                     /locus_tag="VGO32_02450"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEV7199645.1"
                     /translation="MRALAKTRPEPGLEIIDAPAPTPGPGEVQLRIEAASVCGTDRHI
                     YLWDRWAAENIQTPVILGHELAGRVAATGSDVTRVREGDLVGVESHLFDGTCVQCLAG
                     APHLCRDLRVIGAHVDGGFAELVVIPEANAIESNGLDPAVVALQEPMGNAVHAAFVEP
                     IGGRTVAVTGCGPIGLCAVGIAKAAGAAWVVATDTEPYRLELATKMGADLVLDGREPD
                     TVERVLAATGGDGVEVVLEMSGSAAALDQALRFVTRGGRISLLGIFGEPVAVDLSNLV
                     IEKGVRIHGVFGRRIYDTWERTQDLLRSGALDVAPILTHRFDLADWEQGFDLLGSRHA
                     GKVVLLP"
     gene            complement(3469..4419)
                     /locus_tag="VGO32_02455"
     CDS             complement(3469..4419)
                     /locus_tag="VGO32_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7199646.1"
                     /translation="MRLVAFTSADADGPPGRRLGIEKPDGILDLTDLLGSDLGTVLAG
                     ADPVAMLTDAEAETTYEGNALLPMTDVRLLAPLARPGKIVCVGLNYHDHCREQGVAVP
                     ERPTLFAKFANAITDPGIPVVRPRSTEMLDLECELAVVIGWRASQVSVGRAMDHVFGF
                     TILNDVTARDLQREDRQWLRAKGSDGFAPLGPAIVTRDEIGDPANLAIRSWVNGETWQ
                     ESSTADMIWDVPTLLSFISRSITLEPGDIVTTGTPAGVGHFHQPPSYLAAGDVMGCQI
                     AGIGALENPIVDELPRLDDHAAAAGVAELAMPTTASTTDG"
     gene            complement(4424..4849)
                     /locus_tag="VGO32_02460"
     CDS             complement(4424..4849)
                     /locus_tag="VGO32_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199647.1"
                     /translation="MEPATPVTRQRPAAGIVAWFVWVVALLGVTGLLLPRIIAFVDFS
                     ERAPFSLLGILMMAELAVVFFGLTLVLQHKRAGFGFAIGISLLPIPILAGLVPLLLAM
                     PPVAAAGWTTLMVPIGLAITVLLIVTLTRPGARAWFTED"
     gene            4893..5552
                     /locus_tag="VGO32_02465"
     CDS             4893..5552
                     /locus_tag="VGO32_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199648.1"
                     /translation="MPATLPLRPARRTTGRALVLIVVLVATSACIPLPTETPTPSPSP
                     TATPGPTPTPEPSASAEPTPEPYSLADPSATDPREIQVSVAPNLPTDGSGTITVTVVN
                     HSPSLIRELVLRWSTALDEHLLLAPFTPSSQRTCDGCPPLVQPWTKWVVGPGAQGEPA
                     GTTSLGWGPLLSSGGTLTIPVVTTRRQNGPVSFDLQILAGEALLTLEDGTPAWLRVSV
                     P"
     gene            5566..6267
                     /locus_tag="VGO32_02470"
     CDS             5566..6267
                     /locus_tag="VGO32_02470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7199649.1"
                     /translation="MMRGILPAWTEPDPELYRQLGEMVDPGRKVLAGLERIMPLSGKR
                     IADVGTGIGHYPMLLARRTARTYGIESDPALLEEARQRASAAHQPNIRMVEGEPTALP
                     LRDGAVDVVLHGLIEADDAWLPAIGEALRVIRPGGRLVVIGYYGRDDVGALLEPEVVA
                     AARHATQRRSGWWLRHGFQIKVVHARLDLRDVEVAHAILPRLYGDRARAFLMSAHPPQ
                     PELKLGLYHLAKAPA"
     gene            6415..8010
                     /locus_tag="VGO32_02475"
     CDS             6415..8010
                     /locus_tag="VGO32_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7199650.1"
                     /translation="MTATTPQMKITYATLSADNPELQEAFDQAVATARGELGRSYPML
                     IGAELRTSGQTFEHRSPIDTDLVVSRFPLGTRQDVRDAVAAARAAFPAWRDLGWRKRL
                     EIIRRAADLISERQFAYGALMTFEVGKNRLEALGDVEETADLLRYYGQQMEDHDGFVK
                     PMGSLSPAEHTRSILKPHGVWAVISPFNFPMALAGGPASGALVAGNTVVMKPSSDAPL
                     MGWKFVEVLREAGIPDGVVNFVTGPGDTVGAELEENPGIDGMVFTGSYEVGMRLYRSF
                     TRDYPRPIITEMGGKNPAIVMASADLDEAAEGVARSSFGFDGQKCSANSRVYVHRSVA
                     KEFVERLGEQARAIQVGDPTKRENWMGPVINERSLRKFTDAVEEARRDGGTIVAGGSV
                     LDTGDEARGYFPTPTVVSGLPASHRLFRDELFVPFVVVAEVESIDEALRLANDTPYGL
                     TAGIFSRDRSEIEQFLSTIQAGVTYVNRRSGATTGAWPGIQSFGGWKGSGSSGKSGLG
                     PYYVMQFMREQSQTVMGEEPPPA"
     gene            8007..8438
                     /locus_tag="VGO32_02480"
     CDS             8007..8438
                     /locus_tag="VGO32_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HEV7199651.1"
                     /translation="MTGSRRGTAVEADPATTQVVSSGWFAVELRYAYVVDDGAEGADP
                     YLVEDRVVLVDGADEEAAMGHAVELATGYEDEFETDDGLPGTIRFDGIVAIKELDDPP
                     AAGTEVWHEFMSPSGMRTDASGEPLPPVWPAEMAFPRAHDT"
     gene            8461..>9092
                     /locus_tag="VGO32_02485"
     CDS             8461..>9092
                     /locus_tag="VGO32_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016719629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEV7199652.1"
                     /translation="MGWQRDVYEGIRPLFFAADAESIHRWTVTAVRKAAEGSYGRTLL
                     SHLAADGRAGSWSARPVEHMGLQFRNPVGLGAGFDKDGEAIAGWGALGFGFAEVGTVT
                     PVGQPGNARPRVWRLPDDGALVNRMGFNNHGATALADRVARARPWLPPDFVVGVSIGR
                     GAGTGDDAADADYLAAARVVAPVADYLAINVSSPNTAGLLALEEPDRLAAL"
BASE COUNT         1395 a         3233 c         3145 g         1319 t
ORIGIN      
        1 gagggcggcg tagtcctggt cgggggcaag gatccccagg gcgtggtaca gcgcgttgtc
       61 cactgcctgc tgctccgcgg ggtagtcctc cgcgaaccgc tcggccaagc ggcgctcgag
      121 ctcgtcgcgg gtcacgaact ccggtggccc aatatcggcc gccggcaggt cacggatgtc
      181 ggcggtatcg gcctcgatgg cggccagcgc ctcgtcgagc gagggagtcg ccccgctgct
      241 tggctccgcc gacgcaattg gcgtgggcat cggcgtcgcc gtccccggac cgagcgcgat
      301 cgggacgccg agggcacgca gggtcagcac cgccacgagg acgagggcca ggagcagggt
      361 gacgacggcg acgatccgtc gaaggcgtgg gctcatcggt caggcattgt ggggcaaagg
      421 ttcaggtttc ttcacaaatg cgggggttgt cccttcacac atcgatccta ttctcgccgt
      481 gcgagtcaga gatccttccg gatttctcct ccaccctccc tccttcgggc gccggtgtca
      541 ttgggcgggc accggcgctc gtgctatccg gcgacgattc tggggtagca tcgcggaccc
      601 gtggagatca cctggtatgg acgcgcctgc ttccgcctga aaggcaagcg agctgccgtc
      661 atcaccgacc cctgcccccc caagaccggc ttcgtggcag gcaagcacga tgtcgatctg
      721 ctcacgatct cccacgacca tccggaccac acgtacaccc ggtcgatctc ggccggcctg
      781 acgctcaccc gacccggcga gtacgagttt gccgacctgc tggtgaccgg cgtccgaacc
      841 tggcacgacg gggtcgaggg caaggagcga ggggagaacg tgatcttcac cttcgaggtc
      901 gacggcgtcc acgtgtgcca cctgggcgat ctgggccacc tgctgaccga tgagcagctc
      961 cacgagatcg ggccgatcga cgtgctcctc gtgcccgccg gcggccagtt caccatctcc
     1021 cccgccgagg cggccgaggt cgtctcgcag ctggagccca aggtcgtcat tccgatgcac
     1081 tacgcgctcg atggcgggtc gaaggacctg ctcgggcccg aaacgttcct ccacgagctc
     1141 gccaccgagc cgatccgcct gcccaaggcc agcctgacgc cgagcaccct accggaagag
     1201 acccaggtgg tcctgctcga ggcgcgcggc cgcaccgcct agatcagggc cagctcccgg
     1261 ccgaccttct cgaacgcggc gatgcactgg tccaggtcct cgtcggtgtg ctccgcggtg
     1321 acgatcgtcc gtacccggct cttggcttcc ggcacggtcg ggaagccgat cccctgggcg
     1381 aagacgccct cctcgaacag ccggtctgac agcttcatgg cgagcgcgcc cccacccgcg
     1441 atgaccgggg tgatcggtgt ctccgaggca ccggtgtcga atcccagccg gccgaggccg
     1501 tccttgaacc ggcgggcgtt ggcccacagc cgctcgatcc gctccggctc ctgctccagg
     1561 acgtcgatcg ccgccaggca ggcggcggcc acccccggtg gatgggaggt cgagaacagg
     1621 aacggccggg cgcggtggat gagcacctcg cgcagggaca gcgcgccggc cacgtagccg
     1681 ccgaccactc cgatcgcctt ggacagggtg ccgacctgga cgtggacccg gccgtggagg
     1741 tcgaaatggt cgaccgtgcc gcgcccgttg cgacccagca cgcccgacga atgggcgtcg
     1801 tccacgtaca cgatcgcctc catctcctcg gccgcctcca cgatcccggg cagcggggcg
     1861 atgtcgccgt ccatcgagaa cacgccgtcg gtgatgacca ggatcgtccg cccagcgccg
     1921 ccgtcggcgg cccggatctc gcgcaggatg acgcgcagcg catcggtatc cgcgtggggg
     1981 aagaccttac gctcggcctt cgtcagccgg atgccgtcga tgatgctggc gtggttcagc
     2041 tcgtcgctga cgatgacggc cccctcgccg gccaggatcg gtaccgtccc cgtgttggcc
     2101 gtgaacccgg actggaaggt cagggtggcc tcgacctgct tgaactcggc cagccgcgcc
     2161 tcgagctgct ggtggatggt catcgtgccg gcgatcgtcc ggaccgcccc ggatcccgcg
     2221 cccagctcct cggtcgcggc gatcatcgcc gcgcgcagct tcgggtgggt ggtcaggccc
     2281 aggtagttgt tggaggacag cgtgatgacc tcccggccat cgatccggca acgggcggcc
     2341 tgctcgctct cgaccactcg gaggcggcga tacaggccct tggcccggag atcgtcgaga
     2401 tcggcgtcca ggaacgcgag ggggttccgc gagcgaaccg atgtcatggc aggagcacca
     2461 ccttacccgc atgccgcgac cccagcaggt cgaacccctg ctcccagtcg gcgaggtcga
     2521 accgatgggt gaggatgggt gccacgtcca gggcccctga ccgaagcaga tcctgtgtcc
     2581 gctcccacgt gtcgtagatc cggcggccga acacgccgtg gatccgcacc cccttctcga
     2641 tgaccaggtt cgagagatcc accgccaccg gctcaccgaa gatgcccagc aggctgatcc
     2701 gccccccgcg ggtcacgaac cggagcgcct ggtcgagcgc cgcagcggag ccgctcatct
     2761 cgagcaccac ctccaccccg tcaccgccgg tggcggccag cacccgctcc acggtgtccg
     2821 gctcacggcc atcgaggacg agatcggcgc ccatcttggt cgcgagctcg aggcggtacg
     2881 gctcggtatc ggtggccacc acccaggccg cccccgccgc cttggcgatg ccgaccgcgc
     2941 acaggccaat gggaccacag ccggtgaccg ccaccgtccg accgccgatc ggctcgacga
     3001 aggcggcgtg gaccgcgttg cccatcggct cctggagcgc gacgaccgcc ggatcgagcc
     3061 cgttcgactc gatcgcattc gcctccggga tgaccacgag ctcggcaaag ccgccgtcca
     3121 cgtgggcacc gataacccgc aggtcccggc acaggtgggg ggcacccgcg aggcactgga
     3181 cgcaggtccc gtcgaacagg tgcgactcga ctccaaccag gtcgccctcg cggacccgcg
     3241 tcacgtccga tccggtggcc gccacccgac cggccagctc gtggccgagg atgacgggcg
     3301 tctggatgtt ctcggccgcc cagcggtccc agaggtagat gtggcggtcg gtcccgcaca
     3361 cgctggccgc ctcgatccgc agctggacct caccaggccc cggggtcggg gccggcgcgt
     3421 cgatgatctc gagccctggc tccggtcggg tcttggccag ggcccgcatc agccgtcagt
     3481 ggtggacgcg gtggttggca tcgcgagctc ggcgaccccg gcggccgcgg catggtcgtc
     3541 cagccggggc agctcatcca cgatcgggtt ctcgagcgcg ccgatgcccg cgatctggca
     3601 gcccatcaca tcaccggcgg cgaggtagct cggcggctgg tggaagtgac ccacgcccgc
     3661 cggcgtaccg gtggtcacga tgtcacctgg ttccagggtg atcgaccggg agatgaacga
     3721 gagcagggtc gggacgtccc agatcatgtc cgcggtgctg gactcctgcc acgtctctcc
     3781 gttgacccag gagcgaatag cgaggttcgc gggatccccg atctcatcgc gggtcacgat
     3841 cgccggtccc agcggcgcga acccgtcgga tcccttggcc cggagccact gacggtcctc
     3901 gcgttggagg tcacgggccg tgacatcgtt gaggatcgtg aagccgaaga cgtggtccat
     3961 cgcccggccg accgagacct gcgaggcgcg ccagccgatg accacggcca gctcgcactc
     4021 gaggtcgagc atctccgtcg agcgaggacg gacgacgggg atgcccgggt cggtgatcgc
     4081 gttcgcgaac ttggcgaaca gggtcggacg ttccggcacc gccacgccct gctcgcgaca
     4141 gtggtcgtgg tagttcaacc caacgcacac gatcttgccc gggcgcgcga gcggggcgag
     4201 gagccggacg tcggtcatgg gcaggagggc attgccctcg taggtggtct ccgcctcggc
     4261 atcagtcagc atggcgaccg ggtccgcgcc ggcgaggacc gtgcccaggt ccgagcccag
     4321 gagatcggtc agatccagga tgccgtcagg cttctcgatg ccgagccgcc gtcccggtgg
     4381 tccatcggca tcggcggacg tgaaggctac gaggcgcatc agatcaatcc tccgtgaacc
     4441 aggcccgcgc gccagggcgc gtcagggtga cgatgagcag caccgtgatc gcgagcccta
     4501 tggggaccat cagggtggtc catcccgctg cggcgacggg cggcatggca aggagcaggg
     4561 gcacgagacc ggccaggatc gggatgggca gaagcgagat gccgatcgcg aacccgaagc
     4621 ctgcccgctt gtgctggagc accagggtga ggccgaagaa cacgacggcg agctcggcca
     4681 tcatcaggat cccgagcagc gagaacggcg cccgctcgct gaaatcgacg aacgcgatga
     4741 tccgggggag gaggaggccg gtcacgccca gcagggccac gacccagacg aaccaggcca
     4801 cgatccccgc ggcgggccgc tgacgggtga ccggggtcgc gggctccatg ccgggaatcg
     4861 tagcaagggg tcgtccacta ccatgcggcc tcatgccggc cacccttccg ctccgccctg
     4921 cgcgccgcac gacaggccgc gccctggtgc tgattgtggt gctcgtcgcc accagcgcct
     4981 gcattccgct cccgaccgag accccgacgc ccagtcccag cccgaccgcc acgccaggtc
     5041 cgacgcccac cccggagccg agcgccagcg cggagccgac ccccgaaccg tattcgctgg
     5101 ccgatccgtc cgccaccgac ccgcgcgaga tccaggtcag cgtagcgccg aacctgccca
     5161 cggacggatc gggcacgatc acagtgaccg tcgtcaacca ctccccgtcg ctcatccgcg
     5221 agctcgtcct ccgctggtcg accgcgctcg acgagcatct tctcctggcg ccgttcacgc
     5281 cgtcgtcgca gcggacgtgc gacggatgcc cgccgctcgt ccagccgtgg acgaagtggg
     5341 tcgtcggccc cggtgcccag ggcgagccgg cgggcacgac ctcgctgggc tggggcccac
     5401 tcttgtcatc gggtgggacg ctgacgatcc cggtcgtcac cactcggcgg cagaacgggc
     5461 cggtgtcgtt cgacctccag atcctggccg gcgaggcgct gctcaccctc gaggatggga
     5521 cccctgcctg gctccgggtc tcggtgccat gaggatgacc agccgatgat gcgcggcatc
     5581 ctgcctgcct ggacggagcc ggacccggag ctgtaccgcc agcttggcga gatggtcgat
     5641 cccggacgca aggtgctcgc cgggctggag cggatcatgc ccctctccgg gaagcggatc
     5701 gcggatgtcg gcaccggaat cgggcactac cccatgctgc tcgcacggcg gacggcccgc
     5761 acttacggca tcgagtccga cccggcgctg ctggaggaag cgcgccagcg ggcgagcgct
     5821 gcgcatcaac cgaacatccg gatggtcgag ggcgagccga ccgcgcttcc cctccgcgac
     5881 ggtgcggtgg acgtcgtcct ccacggcctg atcgaggccg atgacgcctg gctgccggcc
     5941 atcggcgagg cgctgcgggt gatcaggccc ggcggacggc tggtggtcat cggctactac
     6001 ggacgggacg acgtcggcgc gctgctcgag ccggaggtgg tggctgccgc gcggcatgcc
     6061 acccagcgtc ggtcaggctg gtggctgcgc catggattcc agatcaaggt cgtccatgcc
     6121 cgcctggacc tgcgtgacgt tgaggtggcc catgccatcc tgccccgcct ctacggcgac
     6181 cgcgcgcggg cgttcctgat gagcgctcat ccgccacagc cggagctcaa gctggggctc
     6241 tatcacctcg ccaaggcacc tgcctgaaac ggccacagct cgccatcggg tggggctatc
     6301 atcccgactt gggaggccag gttcttctcc ccccggagcc tggcctcccg ctgtgcccgg
     6361 atccggcggg accggtgcga taatcgcctc cgagccaacc caggagaccg atccgtgacg
     6421 gccacgacgc cccagatgaa gatcacgtac gccaccctga gcgcggacaa ccccgagctg
     6481 caggaggcgt tcgaccaggc cgtcgcaacg gctcgtggcg agctcgggcg ctcgtatccg
     6541 atgctcatcg gcgccgagct gcggacgtcg ggccagacct tcgagcatcg gtcacccatc
     6601 gacaccgacc tcgtcgtcag tcgcttcccg ctgggcaccc gacaggacgt ccgggacgcc
     6661 gtggcggcgg cccgcgcggc attccccgcg tggcgcgacc tgggctggcg aaagcggctc
     6721 gagatcatcc ggcgcgcggc cgacctgatc agcgagcgac agttcgcgta cggcgcgctc
     6781 atgaccttcg aggtgggcaa gaaccggctg gaggcgctgg gcgacgtcga ggagaccgcc
     6841 gacctcctcc gctactacgg gcagcagatg gaggaccacg acggcttcgt caagcccatg
     6901 ggctcgctgt cgcccgcgga gcacacccgc tccatcctca agccccacgg cgtgtgggcg
     6961 gtcatcagcc cgttcaactt cccgatggcc ctcgccggcg gccccgcgtc cggcgccctc
     7021 gttgccggca acacggtggt catgaagccg tcatccgatg cgccactgat gggctggaag
     7081 ttcgtcgagg tcctgcgcga ggccggcatc ccggacgggg tcgtgaactt cgtcaccggg
     7141 ccgggcgaca cggtcggcgc cgagctggag gagaacccgg ggatcgacgg gatggtgttc
     7201 accgggtcgt acgaggtcgg gatgcgcctg taccgcagct tcacccgcga ctacccccgg
     7261 ccgatcatca ccgagatggg tggcaagaac ccggccatcg tgatggccag cgcagacctg
     7321 gacgaggcgg cggagggcgt cgcgcgctcg tcgttcggat tcgacggcca gaagtgcagt
     7381 gccaactcgc gggtctacgt ccatcggagc gtggccaagg agttcgtcga gcgtctcggc
     7441 gagcaagccc gcgcgatcca ggtgggcgac cccaccaagc gtgagaactg gatggggccg
     7501 gtcatcaacg aacgctcgct gcgcaagttc accgatgcgg tcgaggaggc ccggcgcgac
     7561 ggcggcacga tcgtcgccgg cgggtctgtg ctcgacaccg gggacgaggc gcgcggctac
     7621 ttcccgaccc ccaccgtggt cagcggcctg cccgcgagcc accgcctgtt ccgcgacgag
     7681 ctgttcgtcc cgttcgtggt cgtggccgag gtcgaatcca tcgatgaggc ccttcggctc
     7741 gcgaacgaca cgccctacgg gctgacggcc gggatcttct cccgtgaccg gtccgagatc
     7801 gagcagttcc tgtccaccat ccaggccggg gtcacgtacg tgaaccggcg gagcggcgcg
     7861 acgaccggcg cgtggccggg catccagtcc ttcggcggct ggaaaggctc tggctcgtcg
     7921 ggcaagagcg gcctgggccc gtactacgtc atgcagttca tgcgcgagca gtcccagacc
     7981 gtcatgggcg aggagccgcc tccggcgtga ccggatcacg ccgcggcacg gcggtcgagg
     8041 ccgacccggc cacgacccag gtcgtcagca gcggctggtt cgcggtcgag ctgcgctacg
     8101 cgtatgtcgt cgacgacggc gcggaaggag cggaccccta tctcgtcgag gatcgggtgg
     8161 tcctggtcga cggcgccgac gaggaggccg ccatgggcca tgccgtcgag ctcgcgaccg
     8221 ggtacgagga cgagttcgag accgatgacg gcctgccggg caccatccgc ttcgacggca
     8281 tcgtcgcgat caaggagctg gacgacccgc cggcggccgg gaccgaggtc tggcacgagt
     8341 tcatgtctcc cagcgggatg cggaccgatg cctccggcga gcctctcccg ccggtctggc
     8401 cggcggagat ggcctttccc cgcgcccacg acacctgacc gatcagcctg accgcgaccc
     8461 atgggctggc agcgcgacgt ctacgagggg atccggccac tcttcttcgc ggctgacgcg
     8521 gagtccatcc atcgctggac ggtgaccgcc gtgcgcaagg cggcggaggg ctcctacggg
     8581 cggacgctgc tgagccatct cgccgccgac ggccgggccg gctcctggtc ggcgcggccg
     8641 gtggagcaca tgggtctcca attccgcaac ccggtgggac ttggcgcggg cttcgacaag
     8701 gatggggagg cgatcgccgg ctggggcgcc ctcggcttcg gcttcgccga ggtcgggacc
     8761 gtcacccccg tcggtcagcc cggcaacgcg cggccgcgcg tctggcgcct gcccgacgac
     8821 ggggcgctgg tcaaccggat gggcttcaac aaccacggcg ccacagcgct ggcagatcgc
     8881 gtggctcggg cccgaccgtg gctgccgcct gatttcgtgg tcggcgtatc catcggccga
     8941 ggagccggga ccggcgacga cgcggcggac gcggactacc tcgccgcggc gcgggtcgtg
     9001 gccccggtcg cggattacct cgcgatcaac gtcagctccc cgaacaccgc gggcctgctg
     9061 gccctcgagg agccggaccg cctcgcggca ct
//
LOCUS       DASYGU010000094         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_272585, whole genome shotgun sequence.
ACCESSION   DASYGU010000094 DASYGU010000000
VERSION     DASYGU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272585"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /gene="rnpB"
                     /locus_tag="VGO32_02490"
     ncRNA           <1..286
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGO32_02490"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(300..536)
                     /locus_tag="VGO32_02495"
     CDS             complement(300..536)
                     /locus_tag="VGO32_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199653.1"
                     /translation="MAVERSDAPQRTPIPRAGQIRRCSVRHVAITPVPGGREVQVSCL
                     LGGREHPLSLGTFVEARPICNACTAKGIWRADED"
     gene            650..1519
                     /locus_tag="VGO32_02500"
     CDS             650..1519
                     /locus_tag="VGO32_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199654.1"
                     /translation="MFRRGSKPAQSTADVDRQALFEKLALRPESVCPFLGLAAARSEY
                     EEQATDDHRCYAFGDPEPLSGEQQRNVCLQLGYANCPRYLRGVLVIPTDELEALRRPQ
                     QKVPPPPPPATGQTPAQGGDRRRWVVVLLLVLLIGGLGGFLIFGGRQPIAGGPSPTPE
                     PSTPATPASAEPSASVGASLQIPSPDPTPEPGDEFIGYEVTVVPGRNDIFRVDDAGDI
                     VESNHVFFDNFSRAPVDRVVASNDLLHWRVTEGEVLGWSYIRDESGRFFIREVYRGPD
                     GSLRYTVLSESDT"
     gene            1672..2121
                     /gene="mraZ"
                     /locus_tag="VGO32_02505"
     CDS             1672..2121
                     /gene="mraZ"
                     /locus_tag="VGO32_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEV7199655.1"
                     /translation="MERTPFLTGEYRHALDDRGRIAVPARFRNRMAEGATLTRWLDAC
                     LAVFPRDAWAELAEKLRSLPLTSTQAREFGRFMSSGAVDVELDKQGRLLVPSYLRTYA
                     GLEPGEVIVVGVLNRLEIWAPSAWEPYRSRIEDTPEALAEQLQDLGI"
     gene            2215..>2937
                     /gene="rsmH"
                     /locus_tag="VGO32_02510"
     CDS             2215..>2937
                     /gene="rsmH"
                     /locus_tag="VGO32_02510"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV7199656.1"
                     /translation="MGEGHLPVLVDEVVASLALRAGSSIADCTVGGGGHAERLLEAVS
                     PDGRLLGLDADEAAVREASRTLARFGDRAVIRHSNFSALEDVARAEGMVPLDGVLFDL
                     GLSSYQLADARRGFAFTADAPPDMRFDETSGVSALELIDRSSERELAGILETLGEERR
                     AGRIAKAILAARTSGRIVTATDLAAVVASAVPAGGADAGRIHPATRTFQALRIAVNRE
                     LEVLEAGLAASLRVLRPGGRLAV"
BASE COUNT          422 a         1027 c         1023 g          465 t
ORIGIN      
        1 cgcgatcgcc gaaaggcggc gttcgaggaa agtccgagct ccatagagca ggttcgcggg
       61 taacacccgt ccgccgtgag gcgaggacca gcgccacagt gacgatgccc gcgcaagcgg
      121 gagtgaaacg ggcaagctcg agccggagca agaccgaata ggagggcgat gcggccttcg
      181 ggccgcgaca ggtggctcgc cgagccctcg ggtaggtcgc ccagatggat ggccgtcacc
      241 gggcttcggt ccgggacaga actcggctta ccggccgctc tccagggatc tccccgaggt
      301 cagtcctcgt cggcccgcca gatgcccttc gccgtgcagg cgttgcagat cggccgggcc
      361 tccacgaacg tccccagcga cagcggatgc tcgcgacccc cgagcaggca gctgacctgt
      421 acttctcgtc cgccggggac cggcgtgatg gctacgtgtc ggacgctgca gcgacggatc
      481 tgtccggcgc gaggaatcgg agtccgttgc ggcgcgtcgc tgcgttcgac cgccatgaga
      541 tcacgagcct gcgcggcgga ccgggcgtgc tcaatggcgc gttcgtacca tggcccgacc
      601 tgtcgctcgg ctggtcgagt ccggacccgc ggagcggtat cctcgatcca tgttcaggcg
      661 cggcagcaag cctgcgcagt cgacggccga cgtcgatcgc caggcgctgt tcgagaaact
      721 cgccctccgg cccgaatccg tttgtccgtt cctcgggctg gcggcggcgc gctccgagta
      781 cgaagagcag gcgaccgacg atcaccggtg ctacgcgttc ggcgacccgg agccgctctc
      841 cggggagcag cagcggaacg tctgcctcca gctgggctac gcgaactgcc cgcgctacct
      901 ccgcggcgtt ctcgtcatcc cgaccgacga gctcgaagcg cttcggcgcc cgcagcagaa
      961 ggtcccgccg ccgccgccgc cggcaactgg ccagaccccg gcccagggcg gcgatcgccg
     1021 ccgctgggtg gtcgtcctgc tgctggtgct cctcattggc ggcctgggcg gcttcctgat
     1081 ctttggcggc cggcagccca tcgccggcgg accgagcccg actcccgagc catcgacgcc
     1141 ggcgacgccg gcttcggccg agccgagcgc ctctgtcggg gcgtccctcc agatcccgag
     1201 cccggatccc accccggagc cgggtgacga gttcatcggc tacgaggtga ccgtcgtgcc
     1261 aggccggaac gacatcttcc gggtggatga cgccggcgat atcgtggagt ccaaccacgt
     1321 cttcttcgac aacttcagcc gcgccccggt cgaccgggtg gttgccagca acgacctgct
     1381 gcactggcgc gtgaccgagg gcgaggtcct cggctggtcg tacatccgtg acgagtccgg
     1441 acggttcttc atccgcgagg tgtaccgcgg accggacggc tcgctgcgct acaccgtctt
     1501 gtcggagtcc gacacctagc ccgagattcg gtcccgccgc ggcccttccg gtccgtccag
     1561 cccggcctcc accacgcgcg aaaaacacct tgacgggtgg atgttgccct gtatgatggc
     1621 gccctgtggc tcaaagtggg gccaaatggg gaccagtggt agggtgatcg agtggagagg
     1681 accccgttcc ttactggcga gtaccgacat gcgctcgacg atcgcggtcg gatcgccgtc
     1741 cctgcccgct ttcggaatcg gatggcagag ggcgcgaccc tcacccgctg gctcgacgcc
     1801 tgcctggccg tcttcccccg cgacgcgtgg gccgagctgg ccgagaagct gcggagcctc
     1861 ccgttgacca gcacgcaggc ccgagagttc ggccgcttca tgtcatccgg cgcggtcgac
     1921 gtggagctgg acaagcaggg ccgcctgttg gtgccgagct acctgcgcac ctacgccggc
     1981 ctggagccgg gcgaggtgat cgtggtcggc gttctgaacc ggctcgagat ctgggcgcct
     2041 tcggcctggg agccgtaccg ctcgcggatc gaggacacac ccgaggccct ggcggagcaa
     2101 ttgcaggacc tggggatcta aggcccgtgg cgcgatgacg cacgtcacga ccagcccatt
     2161 catgcgtctc cgggccgctc ggcccgtcac cggaccctcg agggacagtc acgcatggga
     2221 gaagggcatc tgccggttct cgtcgacgag gttgtcgcct cgttggccct ccgtgccggc
     2281 agctccatcg cggactgcac ggtcggtggc ggtgggcacg ccgagcggct tctggaggcc
     2341 gtgtctccgg acggccgcct cctcggcctc gatgccgacg aggcggctgt tcgcgaggcg
     2401 tctcgtaccc tggcccgctt cggggatcgg gccgtcattc gccactcgaa cttcagcgcg
     2461 ctcgaagacg tcgcgcgggc cgagggcatg gtcccgctcg acggcgtcct gttcgacctt
     2521 ggcctctcga gctaccagct cgccgacgcg cggcgcggtt tcgccttcac ggcggacgcg
     2581 ccgccggaca tgagattcga tgaaacgagt ggtgtttcgg ctctcgagct catcgaccgc
     2641 tcctcggagc gagagctcgc cggaatcctc gagacgcttg gtgaagaacg ccgtgcaggc
     2701 cgcatcgcga aggcgatcct ggcggcgcgt acgtcgggcc gtatcgtgac ggccaccgac
     2761 ctggcggccg tggtggcgtc tgcagtcccg gccggggggg ccgacgcggg acgcatccac
     2821 ccggccacca ggaccttcca ggccctccgg atcgcggtga atcgggagct cgaggtgctc
     2881 gaagccgggc tggccgcatc gctccgcgtc ctgcgcccgg ggggccggct ggcggtc
//
LOCUS       DASYGU010000095         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1302224, whole genome shotgun sequence.
ACCESSION   DASYGU010000095 DASYGU010000000
VERSION     DASYGU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302224"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGO32_02515"
     CDS             complement(<1..417)
                     /locus_tag="VGO32_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7199657.1"
                     /translation="MPEYDTSTSYPLTLGEEPAFCPACGKPVVERVLEEDHRPRLVCP
                     DGHVTWRNPRLVVGTLPVRDGQVFLARRGIEPGIGLWSYPGGFLEVGESAQEGARRET
                     EEETDLRVEIGRLIGAYSRPHGGVVTLIYEAQVVGGE"
     gene            463..1878
                     /gene="cysS"
                     /locus_tag="VGO32_02520"
     CDS             463..1878
                     /gene="cysS"
                     /locus_tag="VGO32_02520"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7199658.1"
                     /translation="MAASPTPVRLHNSLTRRVEPLEPVHPGRVGIYTCGSTVYRYAHI
                     GNLRTYLFGDLLRRTLEYLGYEVTYVKNITDVGHMRDDATDTGEDRMVIAALEEGKSP
                     ADIAQFYTDAWLEDEALINIRPADVMPRATDHITEMLSLIEVLLDKGLAYESAGNVYF
                     DVSAFPAYGRLSRTRVEAARAGSRVEVESDKRDPADFALWKAAEAGRLMHWPSPWGEG
                     FPGWHIECSAMGMKYLGDRFDIHTGGIDLKFPHHEDEIAQSDGAIGRQVISAWLHGEF
                     LTLADAKMAKSAGNIIRVSELPEKGFEPLVFRYLALTAHYRSKLDFTEEAMHAAASGL
                     GRLRRAVAGVSESQTIDLDAEPMASYRARFVAAISEDLGIPTALAVAHAVAGDHALTP
                     AQRRAQLLDFDRVLGLGLDQPAALETVGLPEGAAPLLERRAAARAQRDFAAADALREE
                     LAAMGVEVRDTPDGQVATRRR"
BASE COUNT          304 a          683 c          629 g          262 t
ORIGIN      
        1 ctcgccgccc accacctggg cctcgtagat gagggtcacc accccaccgt gcggacggga
       61 gtacgcgccg atcaggcgac cgatctcgac tcgcagatcg gtctcctcct cggtttctcg
      121 gcgagccccc tcctgggccg actcgccgac ctccaggaag ccgcccggat agctccacag
      181 cccgatcccc ggctcgatcc cgcgacgagc gaggaacacc tggccgtcgc ggaccgggag
      241 cgtgcccacc accagacgcg ggttgcgcca cgtaacgtgg ccatcggggc agaccagtcg
      301 cgggcggtga tcctcctcca gcacccgctc gaccaccggc ttgccgcagg cgggacagaa
      361 cgccggctcc tcgccgaggg tcagcgggta gctcgtgctc gtgtcgtatt cgggcatcgg
      421 tcagatggta gtccgatccc gaccgataca atccgggccc tgatggccgc atcccccacg
      481 cccgtccgcc tccacaactc gctcactcgc cgggtcgagc ccctcgagcc ggtgcatccg
      541 ggccgggtcg ggatctatac ctgcggcagc acggtgtatc ggtacgcgca catcggcaac
      601 ctgcggacct acctgttcgg cgacctgctc cgccggaccc tggagtacct cggctacgag
      661 gtcacgtacg tcaagaacat caccgatgtc gggcacatgc gcgacgacgc gaccgatacc
      721 ggggaggacc ggatggtgat cgcggcgctg gaggagggga agagcccggc cgacatcgcc
      781 cagttctaca ccgatgcctg gctggaagac gaggcgctca tcaacatccg gccggccgat
      841 gtcatgccgc gggccaccga tcacatcacc gagatgcttt ccctcatcga ggtcctcctc
      901 gacaaggggc tggcctacga gtcggccggc aacgtctact tcgacgtcag cgcctttccc
      961 gcctacggcc ggttgtcccg cacccgggtc gaggccgccc gggccggcag ccgagtcgag
     1021 gtcgagagcg acaagcgcga tcccgccgac ttcgccctgt ggaaggcggc cgaggcgggc
     1081 cggctcatgc actggccatc cccctggggc gagggctttc ccgggtggca catcgagtgc
     1141 tcggcaatgg ggatgaaata cctcggggat cgattcgata tccataccgg cggaatcgac
     1201 ctcaagttcc cgcaccacga ggacgagatc gcgcagtcgg acggggcgat cgggcgccag
     1261 gtcatcagcg cctggctgca cggcgaattc ctgaccctgg ccgacgccaa gatggcgaaa
     1321 agcgcgggca acatcatccg ggtcagcgag ctgcccgaga aggggttcga gccgctcgtc
     1381 ttccgctacc tggcgctgac cgcccactac cggtccaagc tcgacttcac cgaggaggcg
     1441 atgcacgcgg ccgcgtcggg cctgggccgg ctgcggcgag cggtggcggg agtttccgag
     1501 agccagacga tcgacctcga cgccgagccg atggcgagct accgcgcccg gttcgtggcc
     1561 gccatcagcg aagacctggg catcccgacc gcgctggccg tcgcccacgc ggtggcgggt
     1621 gaccacgcgc tcacgcctgc ccagcggcgg gcacagctcc tcgacttcga ccgcgtcctg
     1681 gggctcggcc tggaccagcc ggcggcgctc gagacggtcg ggctgccaga gggcgccgca
     1741 ccgctcctcg agcgacgagc cgccgcgcgc gctcagcggg acttcgcggc cgccgacgcg
     1801 ctccgcgagg aactcgctgc gatgggggtg gaggtccggg acacgccaga cggccaggtc
     1861 gcgacccgcc gccgatag
//
LOCUS       DASYGU010000096         5296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1353586, whole genome shotgun sequence.
ACCESSION   DASYGU010000096 DASYGU010000000
VERSION     DASYGU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353586"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="jag"
                     /locus_tag="VGO32_02525"
     CDS             <1..797
                     /gene="jag"
                     /locus_tag="VGO32_02525"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEV7199659.1"
                     /translation="RAPAPEPQPDVDLAAEMAETAEEASAGQTDETAPTADADLAEAG
                     GLTPVMADEATAPADGRPRSRGRGRDGGSGRGSRESRPPRSDEPRNGDRPEPRPFVPA
                     KPMDELSESERATVDEAKSVLEELLRLMELPGTVEVALGTEAAKLNVVGDDLGILIGR
                     RGEKLASLQHIVNLIVARREGQLNRIMIDVENYRGRREDQLRDVADRAAKRVSQSGKI
                     IQLEAMPAMERRIVHLALAEHDTVRTQSVGVEPNRRVVILPANKVN"
     gene            805..1245
                     /locus_tag="VGO32_02530"
     CDS             805..1245
                     /locus_tag="VGO32_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199660.1"
                     /translation="MAPATGPVRAFGRLGPEGPWVGFESTPTGYQLVVGRTDGAILSD
                     RDLGPEGTRDALVAGAIGFYLSTLDDPPVELEATQADLGTLIRSLIQADPEGAWAHEA
                     EEALDSIDDGLPGDAVALRLQRLLPAGTDPFELLRRRADGPPAR"
     gene            complement(1253..1693)
                     /locus_tag="VGO32_02535"
     CDS             complement(1253..1693)
                     /locus_tag="VGO32_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199661.1"
                     /translation="MFSALNANDRMAAIAAVIVVITGVISVANSWGVLMFLPILAGAG
                     VLFIVFQARVAPTAKLPMTRGTLLLGLGAIAALIWAAVTLQYMGYILTPPILIFDSIQ
                     FIIGLVAAIVMAFAGWRAYQSEKGTAPAAPSSEAPPAPPAPPAA"
     gene            complement(1795..2153)
                     /gene="ssrA"
                     /locus_tag="VGO32_02540"
     tmRNA           complement(1795..2153)
                     /gene="ssrA"
                     /locus_tag="VGO32_02540"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2226..2687)
                     /gene="smpB"
                     /locus_tag="VGO32_02545"
     CDS             complement(2226..2687)
                     /gene="smpB"
                     /locus_tag="VGO32_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV7199662.1"
                     /translation="MSDEHTVALNRRARHDYNVEETVEAGLVLTGSEIKSIRAGRANL
                     AEAYARIEKGEAWLIGAHIAPYEQANQFNHEPTRTRKLLLHRRQIAELLGKTQTKGYT
                     LIPLRLYIRDGLAKLEIGLGRGRKAYDKRRLIAERDARRQLERETKVRVKL"
     gene            complement(2833..4269)
                     /locus_tag="VGO32_02550"
     CDS             complement(2833..4269)
                     /locus_tag="VGO32_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV7199663.1"
                     /translation="MPEPSGPPESDPQPIPTTAPVQDTNRSTTGRTIRYAAVFALAAL
                     VGTTLFIGGYLAASGGGVAGTSSCTAPSEAFAAFCEAYDQLHQEYVDQLDDDKLVEGA
                     LEGMFQYGVADPYSGYMSAEEFQNSLGDLQGTFTGIGAELAIENTENAGDLEACPTLS
                     ETCVLVVVAPIEGSPAEAAGIQSGDIIRAVDGESVEGSTVQDEVTKVRGPDGTDVTLT
                     VERDGEVFDVTITRAEIVVVEVESEMLNDTVGYIALHGFSANSAEQFAEHLGQLLDQG
                     AEQIVFDLRDNPGGYITAARDIASEFVDEGLLFTQESSGEDTIEWEATGDGQATDPAI
                     DVVVLINGGSASASEIVAAALDETDRATLIGEPTFGKNTVQIWDELVNGGGVRITISR
                     WFTPDHNSVAPDGVQPDITAEVPEDTPAGEDPVLDQALAYLATVADGADPSAHVQTDG
                     DGAAAIPVAGRGVGWVPEAGLWAAFAAC"
     gene            complement(4384..5277)
                     /locus_tag="VGO32_02555"
     CDS             complement(4384..5277)
                     /locus_tag="VGO32_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEV7199664.1"
                     /translation="MSFLRFLGFTLVRAGQGFWRNRMMSLAATATVILMLVLLSGLVI
                     VLGGLNSGLAYIESKVGVTARLVDDLEPAAIDQLVSEVSAIPGVATVEYISPETAMQR
                     LEAEYADRGQTLDTGGADISLYSSIEIALTDPSAARPVAAALGEREEIETITTRQEQY
                     DKLLGVTNIVRLAGLSAMILVGLTVVFMIVNTIRIAVFSRSREIEIMRLVGASDAFIR
                     WPFIVEGMLCGLLGAFVTVLLVALVWSPIQPLLIEIFQMPTAVGAQFYTFVAVLILSV
                     GLVVGTLGSWISVRAHLAAGA"
BASE COUNT          834 a         1802 c         1842 g          818 t
ORIGIN      
        1 cccgggctcc ggctcccgag ccgcagcccg acgtcgacct cgcggccgag atggccgaga
       61 cggccgagga ggcctcggca ggccagaccg atgaaacggc accgaccgcg gacgccgacc
      121 tggccgaggc cggcgggctg accccggtca tggccgacga ggccacggct ccggccgacg
      181 gccgaccgcg aagccgtggc cgagggcggg atggtggatc gggccgcgga tcacgcgaga
      241 gccgcccacc ccgctccgac gagccgcgca acggcgaccg gccggagcct cggccgttcg
      301 ttcccgccaa gccgatggac gagctctccg agtcggagcg cgcgaccgtc gacgaggcga
      361 agtcggtcct cgaggagctg ctccgcctga tggagcttcc gggcacggtc gaggtcgcgc
      421 tcggcaccga ggccgccaag ctgaacgtcg tcggtgacga cctcggcatc ctgatcggcc
      481 gccggggcga gaagctggcg agcctccagc acatcgtcaa cctgatcgtg gcccgtcgcg
      541 agggccagct gaaccggatc atgatcgacg tcgagaatta ccgaggtcga cgagaggatc
      601 agctccgcga tgtcgccgat cgcgccgcca agcgtgtctc gcagagcggg aagatcatcc
      661 agctcgaggc catgcccgcc atggagcggc gcatcgtcca cctcgcgctc gccgagcacg
      721 acacggtgcg cacccagagc gtcggcgtcg aaccgaaccg gcgggtcgtg atccttcccg
      781 ccaacaaggt caactagccg ggccatggcg ccggccacgg ggccggtccg cgcgttcgga
      841 cgcctcggcc cggaggggcc gtgggtggga ttcgagtcga ccccgacggg gtatcagctc
      901 gtcgtcggcc gcaccgacgg cgcgatcctc tccgatcgcg acctgggacc ggaggggact
      961 cgcgacgccc tggtcgcggg tgcgatcggg ttctacctct ccaccctcga cgatcctccg
     1021 gtcgagctcg aggccaccca ggccgacctg ggcacgctga tccggtccct catccaggcc
     1081 gatccggagg gtgcctgggc ccacgaggcc gaggaggccc tggactcgat cgacgatggg
     1141 ctgcccgggg acgcggttgc cctccgcctg cagcgcctgc tgccggccgg aactgatccg
     1201 ttcgagctcc tgcgccggcg agcggacggc ccgccggcgc gctgaacgcc ccctaggcgg
     1261 cgggcggagc gggtggcgcg ggtggcgcct cggacgacgg cgcggcgggg gcggtcccct
     1321 tctccgactg ataggcccgc cagcccgcga acgccatgac gatggcggcg accaggccga
     1381 tgatgaactg gatcgagtcg aagatgagga ttggcggagt gaggatgtag cccatgtact
     1441 ggagggtcac cgccgcccag atgagcgccg cgatggcacc gagtcccagc aggagcgtgc
     1501 cgcgagtcat cggcagcttg gccgtcgggg ccacccgtgc ctggaagacg atgaacagga
     1561 cgccggcgcc ggccaggatg ggcaggaaca tcaggacgcc ccatgaattg gcgacgctga
     1621 tgacgccggt gatgacgacg atcacggcgg caatggcagc catccgatcg ttcgcgttca
     1681 gggcggagaa catgggcgat cacctcgaac ggggttgatg ggcggcacga atccgcgcgc
     1741 cgcggaacgg taccgcgcag ggcatcggtc tatcaagacc gaaaacgaag gaaatggtgg
     1801 agatggggga gaatcgaact ccccgtccag gactgagtta ccgggtctca ctacgagcgt
     1861 gtccggctta cttgggtttc gggccggggg cccccaaccg gcggggtgct cctcgtccta
     1921 tccggccgcc tctcggctct ttgtcaccgc tcaccggagt cggcggtgcc gcatctcccc
     1981 tgatgacatc ttcgccgccc ggggagaggg gacggcgtcg atgctcactg actaggcagc
     2041 gagagcaaac tgacggttgc cagttgcggc ttgcccgcgg ttttacgagg ccatgcgggc
     2101 gacctcggct cgcgattccc ggttcctggg ccctgtcgaa acctgacatc cccacggaac
     2161 gccggcggtg gaccggccga tcaaggatac caaacggccc atccgatctc gatcgggccg
     2221 gttggtcaga gcttgacccg gacctttgtc tcccgctcca gctggcgtct cgcgtcgcgc
     2281 tcggcaatga gccggcgctt gtcgtatgcc ttgcgcccac gcccgagccc gatctcgagc
     2341 ttggccaggc cgtcgcggat gtagaggcgg agcgggatga gcgtgtagcc cttggtctgg
     2401 gtcttgccca gcagctcggc gatctggcgt cggtggagga ggagcttccg cgtccgcgtc
     2461 ggctcgtggt tgaactggtt ggcctgctcg tacggcgcga tgtgggcccc gatcagccat
     2521 gcctcgccct tctcgatgcg cgcgtaggcc tcggccaggt tcgcgcgacc cgcccggatg
     2581 ctcttgatct ctgacccggt cagcaccagc ccagcctcga ccgtctcttc gacgttgtag
     2641 tcgtggcggg cgcgacggtt cagggcgacg gtgtgttcgt cggacatcag ttggtcacgt
     2701 cagatggaaa gagccggcct cgcggccggc tcttcttcgc taccacgcga aggctgtgtc
     2761 agtgcaccgc ctcggtggtg gcgcgcttgg aactatccat ctgcacacca cctcctttcg
     2821 tggccgcgga tgctagcacg cggcgaacgc ggcccacaag ccggcttcgg gcacccaccc
     2881 cacgccgcgc ccggcgaccg ggatggcggc cgcgccgtcc ccatcggtct ggacatgggc
     2941 actggggtcg gcgccgtcgg cgacggtggc caggtaggcc agcgcctggt caaggaccgg
     3001 atcctctccg gccggggtgt cctccggcac ctcggccgtg atgtcgggct ggacgccgtc
     3061 aggcgccacg ctgttgtggt ccggggtgaa ccagcgcgag atcgtgatcc ggaccccgcc
     3121 gccgttcacc agctcgtccc agatctggac cgtgttcttg ccgaacgtgg gctccccgat
     3181 gagggtggcc cggtccgttt cgtcgagggc ggcggccacg atctcggacg ccgaagcgga
     3241 gccgccattg ataaggacca ccacgtcgat cgccgggtcg gtcgcctggc cgtcaccggt
     3301 ggcctcccat tcgatggtgt cctcgcccga ggactcctgg gtgaacagga ggccctcgtc
     3361 gacgaactcc gacgcgatgt cccgggcggc ggtgatgtag ccgccggggt tgtcgcgcag
     3421 gtcgaacacg atctgctcgg cgccctggtc gaggagctgg cccagatgct cggcgaactg
     3481 ctccgccgag ttggcgctga agccgtggag tgcgatgtag ccgacagtgt cgttgagcat
     3541 ctcgctctcg acctcgacca ccacgatctc ggctcgcgtg atggtcacgt cgaagacctc
     3601 gccgtcgcgt tcaacggtca gggtcacgtc cgtcccgtcg gggccacgga ccttcgtgac
     3661 ctcgtcctgg accgtgctcc cttccacgct ctcgccatcg acggcacgga tgatgtcgcc
     3721 ggactggatg ccggccgcct cggccggcga gccctcgatc ggcgcgacca cgaccagcac
     3781 gcaggtctcg gacagagtcg ggcaggcctc gagatcaccc gcgttctcgg tgttctcgat
     3841 cgcgagctcg gcaccgatgc cggtgaacgt gccctgcagg tcgcccagcg agttttggaa
     3901 ctcctcggca ctcatgtagc cgctgtacgg atcggccacg ccgtactgga acatgccctc
     3961 cagggcgccc tccaccagct tgtcgtcgtc gagctggtcg acgtactcct ggtggagctg
     4021 gtcgtaggcc tcgcagaagg cggcgaaggc ctcgctgggc gccgtgcagg acgaagtgcc
     4081 ggcgacgccg cccccgctgg cggccaggta gccgccgatg aacagggtgg tgccgaccag
     4141 ggctgccagg gcaaagaccg ccgcgtagcg gatggtccgg ccggtcgtcg accggttcgt
     4201 gtcctggacg ggggccgtgg tcgggatggg ctgaggatca gattcgggcg ggcccgaagg
     4261 ttcgggcaag cccgaagggg ggttgtcatc catagtgggg acggagagta gcaccggtcc
     4321 ggtcaaggcc cggtattggc cagatgccgt tctcgtaacc gatggtcgcg gaaccggtcg
     4381 aggtcaggct ccagccgcga ggtgggcgcg gacgctgatc cagctgccga gggtgccgac
     4441 cacgagcccg accgacagga tgagcaccgc aacgaacgta tagaactgcg cgcccacggc
     4501 ggtgggcatc tggaagatct cgatcaggag cggctggatc gggctccaca ccagcgccac
     4561 cagcaggacc gtgacgaacg cgcccagcag cccgcagagc atgccctcca cgatgaacgg
     4621 ccagcggatg aacgcgtcgc tggcgccgac gagccgcatg atctcgatct cgcggcttcg
     4681 cgagaagacc gcgatccgga tcgtgttcac gatcatgaac accaccgtca gcccgaccag
     4741 aatcatggcc gacaggccgg ccaggcgcac gatgttcgtc acccccagca gcttgtcgta
     4801 ctgctcctgg cgagtcgtaa tggtttcgat ctcctcgcgc tcgccgagcg cggcggcgac
     4861 cggtcgtgcc gcggacggat cggtcagcgc aatctcgatg ctgctgtaca ggctgatatc
     4921 ggcgccaccg gtgtcgaggg tctgaccccg gtcggcgtac tctgcctcga gccgctgcat
     4981 ggcggtctcg ggcgagatgt attccaccgt cgccacgccc gggatcgcgc ttacctcgct
     5041 caccagctga tcgatcgccg ccggctccag gtcatccacc agtcgggcgg tgaccccgac
     5101 cttgctctcg atgtaggcca ggccggagtt caggccgccg agcacgatca ccagtcccga
     5161 gagcagcacg agcatgagga tcacggtcgc ggtggcggcc agcgacatca tccggttgcg
     5221 ccagaagccc tggccggccc gaaccagggt gaaacccagg aacctcagga acgacatgtc
     5281 gcgcgcatcc taccgg
//
LOCUS       DASYGU010000097         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1713494, whole genome shotgun sequence.
ACCESSION   DASYGU010000097 DASYGU010000000
VERSION     DASYGU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713494"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1504
                     /locus_tag="VGO32_02560"
     CDS             98..1504
                     /locus_tag="VGO32_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV7199665.1"
                     /translation="MTLLELATYAVYLVASVSFILALKFLASPRTARTGNLLGAGGMA
                     LVVAWTIATEPGLLDNLWVLVVGGLVGTVIGVIGARRVPMTAMPQMVAIFNGAGGGAA
                     AVVAVSEFLTATSDSGGELLPTIPVIATLLGAVIGSLSLTGSVVAFGKLQGFIAPRPV
                     TYPGSRFVAAALLAALLIMGVALAFVTNDVTLFLVFVALALIGGVLIVMPIGGADMPV
                     VISLLNSYTGIAVAATGFVLGNYALLIAGTLVGASGAILTQLMCRAMNRSLLNVLFGA
                     FGTAGGAQVAGAAGDQNIRTINAEDAAVLMAYGKSVIIVPGYGMAVAQAQTPVRELMD
                     LLLARGVDVQFAIHPVAGRMPGHMNVLLAEANVPYERLKEMDEINDDFANADVALVIG
                     ANDVTNPAARTDTTSPIYGMPILNVDHAQNVIVLKRSMAAGFAGIDNPLYGDPKTAML
                     FGDAKATVQALVAGVKAA"
     gene            1533..2012
                     /locus_tag="VGO32_02565"
     CDS             1533..2012
                     /locus_tag="VGO32_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199666.1"
                     /translation="MSEPTPDCTLCRGIDGDPELGRVEVWRDDLWRLTAATDSEVPGF
                     AFLEPIRHIPHVTDLGGPEAETFGAVLGRVTTALKEATGTELVYVYVFGGGIPHFHVH
                     LAPHREGDALSTQLIRGEIVEEHLPSGATRVTSLDFPPIPQPELRAVADRVRGLLAD"
     gene            complement(2009..3286)
                     /locus_tag="VGO32_02570"
     CDS             complement(2009..3286)
                     /locus_tag="VGO32_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199667.1"
                     /translation="MGLFPNAWTVTLREFRLRVRSRVYLVVTIILAVVGMGLALLPVA
                     LGALGLDEPSSVSVYAASPDAEESAAALVASLNAMEDAGVEATLVDDDADARDAVESG
                     DLDGLVTVDRAADQDLTFDLFTDEGATSGLVFTVNSAASQVAVADRLVRAGVDPSQVG
                     EIFAPTAFEVTPVDPDAGGEPDNLAPRYLIATALVVLMFMAIITYGQWVATSVAEEKA
                     SRVMELLITAATPRQLLLGKVLGAGGAGLIQYIAVVGAALLGLVLAGPLSQALSGDSG
                     SPAPDLSALNAGVLLVVGLFFVGGFGLYATLYAALGSTASRQEEVQNITGPMIIVGVA
                     GYLIAFAALNVPDADWVKVLSFVPFFSPYLIPTRMVMGEIAPWEIGLSFLFLILGALA
                     ALWVAARIYSAGVLLYGQKAGLRSVWRAVRVNR"
     gene            complement(3286..4245)
                     /locus_tag="VGO32_02575"
     CDS             complement(3286..4245)
                     /locus_tag="VGO32_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199668.1"
                     /translation="MSLVVDTISKRFGDIVALDGVSFTAEPGRIFGLLGANGAGKTTS
                     MRIVLDILRADSGSVTWQGKPNIEVPRRTWGYLPEERGLYLQMTVIDVLRFFAALYGV
                     PERESTAEVEDWLERFRIPDYRDRKVQELSKGNQQKIQFIAAILHDPDVLIMDEPFSG
                     LDPVNVQLLKEAFLEMRRRGKVLIFSTHQMETVEELCESIVIVDRGRIVVGGHVRDVK
                     RAMNRQVVRIATDGHNGTDGLAWVESMPGVTLTRRGQDYLELHVHSDTDPSSLLNAAL
                     ERGEKVTRFEITDPSLEEVFIEHVGRPAELAEDRHLAPGQERS"
     gene            4317..4994
                     /locus_tag="VGO32_02580"
     CDS             4317..4994
                     /locus_tag="VGO32_02580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7199669.1"
                     /translation="MTEWNGRWVVFDVGETLVDETRVWSSWADVTGIPRLTFMAVLGA
                     TIARGGAHQSVFRELGLPHWEHHRDQLEASYGAIVGDDLYPDVRPTLSALTVAGYRLA
                     IIGNQPARRTADLQALDLPVEVIAMSDEMGVAKPDPAYFARVLELLGEPDPGVVAHVG
                     DRVDNDVLPAAAAGLHSIWIRRGPWGKIQQLPPDIVPALVIDSLVELPDGLEQAFDSL
                     PSPMTSV"
     gene            5091..>5342
                     /locus_tag="VGO32_02585"
     CDS             5091..>5342
                     /locus_tag="VGO32_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7199670.1"
                     /translation="MDELATVLFQLFVLLAATKLGDELFKRIGQPPLVGEILAGVIVG
                     PALLGWYQITPETELFAQIGAVLLLFEVGVHTRVGDLVRV"
BASE COUNT          803 a         1916 c         1783 g          840 t
ORIGIN      
        1 atgttgttgg tggcttcgtc gtcaccgacc ggatgctcga gatgttccgc ggccggcggg
       61 acggcggtcg cggggatggc tcgccgggag acaaggcgtg accctgctcg agctggcgac
      121 ctacgccgtc tacctcgtcg catcggtcag cttcatcctg gcgctgaagt tcctcgcgtc
      181 gccgcggacg gcgcgcaccg ggaacctgct gggagccggc ggcatggccc tcgtcgtggc
      241 atggaccatc gccaccgagc ccggcctgct cgacaacctg tgggtgctgg tcgtgggcgg
      301 actggtgggc accgtgatcg gtgtgatcgg tgcccgccgg gtgccgatga ccgcgatgcc
      361 ccagatggtc gccatcttca acggcgcggg cggcggagca gccgcggtcg tcgccgtgtc
      421 cgagttcctg accgccacca gcgattccgg cggcgagctg ctgcccacca tcccggtgat
      481 cgccaccctc ctcggcgcgg tcatcggctc gctgtcgctg accggatcag tcgtcgcctt
      541 cggcaagctg cagggcttca tcgccccccg cccggtcaca tatcccggct cccggttcgt
      601 ggccgccgcc ctgctcgccg cgctcctgat catgggcgtg gcgctcgcct tcgtcaccaa
      661 cgacgtcacc ctgttcctcg tcttcgtggc cctggccctg atcggcggcg tcctgatcgt
      721 gatgcccatc ggcggcgcgg acatgccggt ggtcatcagc ctcctgaact cgtacaccgg
      781 catcgcggtg gcggccacag gcttcgtgct gggcaactac gccctgctca tcgccggaac
      841 cctggtcggc gcgtcgggcg cgatcctcac ccagctcatg tgccgggcca tgaaccgctc
      901 gctgctcaac gtcctgttcg gggcgttcgg cactgctggc ggcgcacagg tcgccggcgc
      961 ggcgggggac cagaacatcc gaaccatcaa cgccgaggac gccgcggtcc tgatggccta
     1021 cggcaagagc gtcatcatcg tgccgggcta cgggatggca gtcgcccagg cccagacccc
     1081 ggtccgcgag ctcatggacc tgctcctggc gcgcggcgtc gacgtccagt tcgccatcca
     1141 cccggtcgcg ggccggatgc cgggccacat gaatgtcctc ctcgccgagg ccaacgtccc
     1201 gtacgagcgg ctcaaggaga tggacgagat caatgacgac ttcgccaacg cggacgtggc
     1261 cctggtcatc ggcgccaacg acgtgaccaa cccggcagcc cggacggaca cgacctcccc
     1321 gatctacggc atgcccatcc tgaacgtgga ccacgcccag aacgtgatcg tcctcaagcg
     1381 gagcatggcg gccggctttg cgggcatcga caacccgctg tacggcgacc cgaagacggc
     1441 gatgctgttc ggcgacgcca aggcgacggt ccaggccctg gttgccgggg tgaaggccgc
     1501 ctaagtcgcc aacccaggcg ccgcggttac cgatgagcga gccgacgccc gactgcacac
     1561 tctgccgcgg catcgatggc gaccccgagc tgggtcgggt cgaggtctgg cgcgatgacc
     1621 tgtggcggct cacggcggcc accgattcgg aggtcccagg cttcgccttc ctcgaaccca
     1681 tccgccacat cccacatgtc acggatctcg gcggccccga ggccgagaca ttcggcgccg
     1741 tcctgggcag ggtcacgacc gcgctgaagg aggcgacggg caccgagttg gtctacgtct
     1801 acgtctttgg cggcggaatc ccgcatttcc acgtccatct cgctccccac cgcgagggcg
     1861 acgcgctgag cacgcagctc atccgcggcg agatcgtcga agagcaccta cccagcggcg
     1921 cgacccgggt cacaagcctg gactttccgc cgatccccca gccggagctg cgtgcggtgg
     1981 ccgaccgcgt ccggggcctg ctcgccgact agcggttgac ccggaccgcg cgccacacgc
     2041 tgcgcagccc ggccttctgg ccgtacagca gcaccccggc cgagtagatc cgggcagcca
     2101 cccacagcgc cgccagtgcg ccaaggatca ggaacaggaa tgacaggccg atctcccacg
     2161 gggcgatctc gcccatcacc atccgggtcg ggatcaggta cgggctgaag aacggaacga
     2221 aggacaggac cttgacccag tccgcatcgg gcacgttcag tgcggcgaac gcgatcaggt
     2281 agccggccac gcccacgatg atcatcggcc cggtgatgtt ctggacctcc tcctgccggc
     2341 tcgcggtcga gcccagggcc gcgtacaggg tggcgtacag gccgaagccg ccgacgaaga
     2401 acagaccgac gacgagcagg acacccgcgt tcagggcgct gaggtccggc gctgggctgc
     2461 cgctgtcccc gctcaacgcc tggctcagcg gcccggccag cacgagcccc aggagcgccg
     2521 cgcccacgac cgcgatgtac tggatcaggc ccgcgccgcc cgcgcccagc acctttccca
     2581 gcagcagctg gcgcggagtc gcggccgtga tgagcagctc catcacccgg ctcgccttct
     2641 cctccgccac gctggtggcc acccactggc cgtaggtgat gatcgccatg aacatgagaa
     2701 ccaccagcgc ggtcgcgatc aggtagcgcg gggcgaggtt gtccggttcc cctcccgcgt
     2761 cggggtcgac cggcgtcacc tcgaacgcgg tcggcgcgaa gatctcgcct acctggctcg
     2821 ggtccacgcc ggcccgcacc aggcgatccg ccaccgcgac ctgcgacgcg gcgctgttta
     2881 ccgtgaacac caggccgctg gtcgcccctt catcggtgaa caggtcgaag gtcaggtcct
     2941 ggtcggcagc ccggtcgacg gtgaccaggc cgtcgaggtc gccggattcg accgcgtcgc
     3001 gagcgtcggc gtcatcgtcg acgagcgtgg cctccacgcc cgcgtcctcc atcgcgttca
     3061 acgacgcgac gagggccgcc gcggactcct cagcgtctgg cgaggcggcg tagaccgata
     3121 ccgatgacgg ctcgtcgagc cccaaagcac cgagcgcgac cggcagcagg gcgaggccca
     3181 tcccgaccac ggccaggatg atcgtgacca ccaggtacac ccggctccgg acccggagcc
     3241 ggaactcgcg cagggtcacc gtccaggcgt ttgggaagag gcccatcagc tccgctcctg
     3301 gcccggcgcc aggtggcggt cctcggccag ctcggctggc cggcccacgt gctcgatgaa
     3361 gacctcttcg agcgacgggt cggtgatctc gaaccgggtc accttttcgc cccgctccag
     3421 ggccgcgttc aggagcgacg acggatcggt gtcggagtgg acgtggagct cgagatagtc
     3481 ctggccccgc cgggtgagcg tcacgcccgg catggactcg acccatgcca ggccgtccgt
     3541 cccgttgtgg ccgtcggtgg cgatgcgcac gacctgccgg ttcatggcgc gcttcacgtc
     3601 gcggacgtgg ccgccgacca cgatcctgcc ccgatccacg atcacgatcg actcgcacag
     3661 ctcctcgacg gtctccatct ggtgcgtcga gaagatcagc accttgcccc gtcggcgcat
     3721 ctcgaggaag gcctccttca ggagctggac gttgaccggg tccagaccgc tgaacggctc
     3781 gtccatgatc aggacgtcgg ggtcgtggag gatggcggcg atgaactgga tcttctgctg
     3841 attgcccttc gacagctcct ggaccttccg gtcgcggtag tcggggatcc ggaaccgctc
     3901 cagccagtcc tccacctccg ccgtggactc ccgttcgggg acgccgtaca gcgccgcgaa
     3961 gaagcggagg acgtcgatga ccgtcatctg gagatacagg ccccgctcct cgggcaggta
     4021 gccccaggtc cgtcgcggca cctcgatgtt cggcttgccc tgccaggtca ccgagccgga
     4081 gtccgcgcgc aggatgtcca gcacgatccg catcgaggtc gtcttgcccg cgccgttcgc
     4141 gcccagcagg ccgaagatgc ggcccggctc cgcggtgaag ctcaccccgt cgagggcgac
     4201 gatgtcgccg aatcgcttcg aaatcgtgtc gaccacgagg ctcatggcgg gccgatgcta
     4261 cccgctcacc gtcgcactcg tgtcaacgcc tccggcgagc cggtagagtc cgacccatga
     4321 ccgaatggaa cggccgatgg gtggtcttcg acgtcggcga gacgctggtc gacgagaccc
     4381 gggtgtggtc ctcctgggcc gacgtgacgg ggattcctcg cctcaccttc atggccgtcc
     4441 tgggggccac catcgcccgc ggcggcgccc accagagcgt cttccgggag ctcggcctgc
     4501 cgcactggga gcaccaccgc gaccagctcg aggcgtccta tggagccatc gtcggggacg
     4561 acctgtaccc cgacgtccgg ccgacgctgt ccgcgctcac ggttgcgggc tatcggctgg
     4621 cgatcattgg caaccagccc gcccggcgaa cggcggatct ccaggccctg gacctgccgg
     4681 tcgaggtgat cgcgatgtcc gacgagatgg gcgtcgccaa gccggacccc gcctatttcg
     4741 cgcgcgtcct cgagctgctt ggcgagccgg acccaggcgt ggtcgcccac gtcggtgaca
     4801 gggtggacaa cgacgtcctg cccgcggcgg cggccgggct ccactcgatc tggatccgac
     4861 gtggcccgtg ggggaagatc cagcagctgc cgccggacat cgtcccggct ctcgtcattg
     4921 actcgctggt ggagctcccc gacggcctgg agcaggcatt cgattccttg cccagcccga
     4981 tgacatcggt ctagactcgc cggtgggcgg gaccgggccc gtatcgccgg tcgaggtgga
     5041 gactgcgcag ccggtgcgca gtttttctgt ttctggacga ggtgcagcca gtggatgaac
     5101 tggcgacggt cctgttccag ctcttcgttc tgctggccgc cacgaagctg ggcgacgagc
     5161 tgttcaagcg catcggccag ccgcctctgg tcggggagat cctggccggt gtgatcgtgg
     5221 gcccggcgct gctgggctgg taccagatca cgcccgagac cgagctgttc gcgcagatcg
     5281 gcgccgtcct gctcctgttc gaggttggcg tccacacccg ggtcggcgac ctagtgcggg
     5341 tc
//
LOCUS       DASYGU010000098         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1765126, whole genome shotgun sequence.
ACCESSION   DASYGU010000098 DASYGU010000000
VERSION     DASYGU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765126"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VGO32_02590"
     CDS             <1..420
                     /locus_tag="VGO32_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV7199671.1"
                     /translation="VLGTIRLQRTNRGIVASGRARTLARWTCIRCLDEFDEPVEVEIN
                     EEFLPTMDPTTGAVVVVDAADVQVARVDAHHEIDLAGVVNDELTLAEPMHPLCRPDCG
                     GLCPICGLRRDDGVHDHPDTEIDPRLAGLADWKPPAE"
     gene            493..696
                     /gene="rpmF"
                     /locus_tag="VGO32_02595"
     CDS             493..696
                     /gene="rpmF"
                     /locus_tag="VGO32_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV7199672.1"
                     /translation="MGVPKRKVSKSRKGERRAHLALRAPTLVECDHCHELKRAHTVCP
                     TCGWHNGREVVPPPAPTPTESGR"
     gene            708..1793
                     /gene="plsX"
                     /locus_tag="VGO32_02600"
     CDS             708..1793
                     /gene="plsX"
                     /locus_tag="VGO32_02600"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV7199673.1"
                     /translation="MRIAVDAMGGDLAPREIVRGAINYAATHADEVILVGDVPRIERE
                     IDEYGRGRPASVSFVDAPEVVEMGEAPAAGVRRKRSSIRVATDLVRDGAADAVVSAGS
                     TGASMAAAVFRLGRVEGIDRPALPAYFLTPSGPLVLLDVGANVDSDAQNLVQFAAMGS
                     LYAERVLHVTNPRVALLNIGEEPEKGDALTREAYGRFQGSDLNFVGNVEPHELASHPA
                     DVVVCDGFVGNVVLKLTEGLTSYIFRSIREDLLRGPVAPIAVLALRPGIDRLRHRFDY
                     EQFGGAPLLGVRGVSIVTHGRAKARMVENAIRVGSEAAAADIPAVIADWSRRHPALYA
                     GSPSANGAENSAPAPDEPAAVPIEPAP"
     gene            1839..2225
                     /locus_tag="VGO32_02605"
     CDS             1839..2225
                     /locus_tag="VGO32_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HEV7199674.1"
                     /translation="MVALFEAEFPPQQADAALTRLAAEWGAEPEVVAHAREIVAGVRR
                     HQAALDAEIATRAPKIPVSELGRIERTILRSALFEVLYSAAIPSAGTIRDAVSLARTY
                     AGESAARLIGGVLRGVARPESEGDQG"
     gene            2222..2464
                     /gene="acpP"
                     /locus_tag="VGO32_02610"
     CDS             2222..2464
                     /gene="acpP"
                     /locus_tag="VGO32_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV7199675.1"
                     /translation="MTDGTTFERLKKIIVEQLGVEPDDVTPEAAFVEDLNADSLDLVE
                     LIMSLEEEFGMEISDEDAEKIQKVSDAVEYIEERQQ"
     gene            2472..3176
                     /gene="rnc"
                     /locus_tag="VGO32_02615"
     CDS             2472..3176
                     /gene="rnc"
                     /locus_tag="VGO32_02615"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV7199676.1"
                     /translation="MSSPTISDLGERLGLVFHDTDAIGQAFVHASYANENPDLVAGNN
                     ERLEFLGDAVLGVIVSHFLYERFPEADEGGLTAFRASIVNRSSLATLAAEIELDRHLL
                     MGRGELESGGARRPSLLAGVFEALVGAVYLDLGFDATRAAFEPLLLTRLPDADQVDAP
                     KSAKSRLQEWSQQHGGPRPIYELVASEGPAHAQRFTVNVAVGTDLLASGSGSSRQRAE
                     ERAAQAALDHLAQVNA"
     gene            3173..>4191
                     /locus_tag="VGO32_02620"
     CDS             3173..>4191
                     /locus_tag="VGO32_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7199677.1"
                     /translation="MSRLKELKLHGFKSFAEPTRFVFQPGVTAVIGPNGSGKSNMADA
                     IRWVLGEQSNRSLRTRRSDDVIFAGSESRKPIGMAEVTLTFDNHDGWLPIDFEEVTIA
                     RRAYRSGDPEYLINGTRARLRDVVELLAGGRLGANELVVVGQGTVDAALSLRPEERRQ
                     LFEEAAGVKGLQVRRNEAQARLGRARDNLTRVSDLVRELKPQVKRLALQAEHQEAHDR
                     LGRRARALVLEHHARRAQTIQTSLGEARRRGAALAQEVEADRGSQAAGRQAVAAAEEQ
                     YWAAEQAGRQAAADWEAAREAVIRAESRHGALVERDIDLKAEAERTTRELGDMTERAG
                     TEAQP"
BASE COUNT          657 a         1477 c         1474 g          583 t
ORIGIN      
        1 gtgctgggca ccatccgcct ccagcgcacg aaccgaggca tcgtggcctc cggccgagcc
       61 cggaccctgg cccgctggac ctgcatccgc tgtctcgacg agttcgacga gccggtggag
      121 gtggagatca atgaggagtt cctgcccacc atggacccga cgaccggcgc ggtcgtggtg
      181 gtggacgccg cggacgtgca ggtggcacgg gtcgatgccc accacgagat cgacctggcc
      241 ggggtcgtga acgacgagct caccctggcg gagccgatgc acccgctgtg ccgtcccgac
      301 tgcggcggcc tgtgcccgat ctgcggcctt cgtcgcgacg acggcgtcca tgaccatccc
      361 gacaccgaga tcgatccgcg gctggccggg ctggccgact ggaaaccgcc cgccgagtga
      421 cgcatgtggt ccacgccccg gaccaccgct atactgcggc ctcctcatcc cgattctgca
      481 caggagagcc gcgtgggcgt tcccaagcgc aaggtctcga aatcccggaa gggcgaacgt
      541 cgcgcccatc tcgcgttgcg agcgccgacc ctggtcgagt gcgaccactg ccacgagctg
      601 aagcgcgcac ataccgtgtg cccgacctgc ggctggcata acggccgcga ggtggtgccg
      661 ccgccggcgc ccaccccgac cgagtccggc cgctgacggt cggcccgatg cgcatcgcgg
      721 tcgacgcgat gggcggagat ctcgctccgc gtgagatcgt ccggggggca atcaactacg
      781 ccgccaccca cgccgacgag gtcatcctcg tgggtgacgt gccgcgcatc gagcgcgaga
      841 tcgatgagta cggccgcggc cgtccggcga gcgtgtcctt cgtcgatgcg cccgaagtcg
      901 tcgagatggg ggaggcaccg gccgccggcg ttcgacggaa gcgctcctcg atccgggtcg
      961 cgaccgatct cgtccgcgac ggcgcggccg atgcggtggt cagcgccggc tcgaccggcg
     1021 ccagcatggc cgccgccgtc ttccgcctcg ggcgggtcga ggggatcgat cgaccggccc
     1081 tgcccgccta cttcctgacg ccctccggcc ccctcgtcct cctcgacgtg ggcgccaacg
     1141 tcgactcgga tgcgcagaac ctcgtccagt tcgccgcgat gggctcgctg tatgccgaac
     1201 gcgtgctcca cgtgaccaac ccgcgggtcg cgctgctgaa catcggcgag gagccggaga
     1261 agggcgatgc gctgactcgg gaggcctacg gccggttcca gggatcggac ctgaacttcg
     1321 tgggcaacgt cgagccgcac gaactggcca gccatccggc ggatgtggtc gtgtgcgacg
     1381 ggttcgtggg caacgtcgtc ctcaagctca ccgaggggct gacctcgtac atcttccgct
     1441 ccatccgtga ggacctcttg cgcggcccgg tcgcgcccat cgcggtcctc gcgcttcgcc
     1501 cgggcatcga ccggctccgt caccggttcg actacgagca gttcggcggc gccccactcc
     1561 tcggcgtccg tggggtgagc atcgtgaccc acggtcgggc caaggcacgg atggtggaga
     1621 acgcgatccg cgtcgggtcc gaggcggcgg cggccgacat cccggccgtc atcgcggact
     1681 ggagccgacg gcacccggcg ctgtacgccg gatcgccgtc cgccaacggt gccgagaact
     1741 cggcgccggc gccggacgag ccggccgccg ttcccatcga accggccccc taggtggccg
     1801 cacgcgagcg gcccccgaga cgggcccgca agctggcgat ggtggccctg ttcgaagccg
     1861 aattcccgcc tcagcaggcg gacgccgccc tgacccggct cgcggccgag tggggcgccg
     1921 agccggaggt ggtggcccac gcgcgggaga tcgtggccgg cgtgcgccgt caccaggccg
     1981 cgctcgacgc cgagatcgcg acccgggcgc cgaagatccc ggtctccgaa ctcggccgaa
     2041 tcgaacggac gatcctcagg agtgccctat ttgaggtgct atactccgcc gcgattccga
     2101 gcgccgggac gatccgcgac gcggtctctc tcgctcgaac ctacgctggg gaatcggccg
     2161 cccgactcat cggtggcgtg ctccgaggcg tggccagacc cgaatctgag ggagaccaag
     2221 ggtgaccgac ggcaccacgt tcgagcggct caagaagatc atcgtcgagc agctcggggt
     2281 cgaacccgat gacgtcacgc cggaggcggc atttgtcgag gacctgaacg ccgactcgct
     2341 cgacctcgtg gagctcatca tgagcctcga ggaagagttc ggcatggaga tctcggacga
     2401 ggatgccgag aagatccaga aggtctccga cgccgtcgag tacatcgagg aacgccagca
     2461 gtaggttccc cgtgagctca cccaccattt ccgacctggg tgagcgactc ggcctcgtct
     2521 ttcacgatac cgacgccatc ggccaggcat tcgtccatgc ctcgtatgcc aacgagaacc
     2581 ccgacctcgt cgccggcaac aacgagcgcc tcgagttcct cggcgacgcc gttctgggcg
     2641 tgatcgtcag ccacttcctg tacgagcggt ttcccgaagc cgatgagggc ggcctcaccg
     2701 cgttccgggc ctcgatcgtc aatcggtctt ccctggcgac gctcgccgcg gagatcgagc
     2761 tcgaccgcca cctgctgatg ggccgaggag agctcgagag cggcggcgcc cgacggccat
     2821 cgctcctggc cggggtgttc gaagcgctgg tgggagcggt gtacctcgac ctcgggttcg
     2881 acgcgacccg cgcggcgttc gagccgctcc tgctcacccg cctgccggac gccgatcagg
     2941 tagacgcacc gaagtcggcc aagagccggc tccaggagtg gagccagcag cacggcggcc
     3001 cacgtccgat ctacgagctg gtcgcgtccg agggcccggc ccatgcccag cggttcacgg
     3061 tcaacgttgc cgtcgggacc gacctcctgg catcgggatc ggggagcagc cgccagcggg
     3121 cggaggagcg cgcggcgcag gctgcgctcg accatctcgc ccaggtgaac gcgtgagccg
     3181 cctgaaggag ctcaagctcc acggcttcaa gagctttgcc gagcccaccc ggttcgtctt
     3241 ccagcccggg gtgaccgcgg tgatcggtcc caacggcagc ggcaagagca acatggcgga
     3301 cgccatccgc tgggtgctgg gcgagcagtc gaaccgatcc ctccgcaccc ggcgctcgga
     3361 cgacgtgatc ttcgccggct cggagagccg caagccgatc ggcatggccg aggtgacgct
     3421 caccttcgac aaccacgacg ggtggctccc gattgacttc gaggaggtca cgatcgcccg
     3481 gcgggcgtat cggtccggcg acccggagta cctcatcaac ggcacacgag cccggcttcg
     3541 tgacgtggtg gagctgcttg ccggcggacg gctgggcgcg aacgagctcg tggtcgttgg
     3601 ccagggcacc gtcgatgcgg cgctgtccct ccggcccgag gagcgacgcc agctgttcga
     3661 ggaagcggcc ggggtgaagg gactccaggt tcggcgcaac gaggcccagg cgcggctggg
     3721 gcgggcacgc gacaacctga cccgcgtgtc ggacctcgtt cgcgagctca agccgcaggt
     3781 caagcggctc gcgctgcagg ccgagcacca ggaagcccac gaccggctcg ggcgtcgtgc
     3841 ccgcgcgctc gttctcgaac atcacgcgcg gcgtgcacag accatccaga ccagcctggg
     3901 tgaggctcgg cgacgcgggg ccgcgctggc ccaggaggtc gaggccgacc ggggttcgca
     3961 ggccgccggg cgccaggccg tggccgcggc cgaggagcag tactgggccg ccgaacaggc
     4021 cggacggcag gccgcagccg actgggaggc cgcgcgcgag gccgtcattc gggccgagag
     4081 ccggcacggt gcgctcgtgg aacgggatat cgacctgaag gcggaggcgg agcgcacgac
     4141 gcgcgagctg ggcgacatga ccgagcgagc cggaaccgaa gcgcagcccg a
//
LOCUS       DASYGU010000099         8739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1559278, whole genome shotgun sequence.
ACCESSION   DASYGU010000099 DASYGU010000000
VERSION     DASYGU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559278"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGO32_02625"
     CDS             <1..438
                     /locus_tag="VGO32_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV7199678.1"
                     /translation="YGLIPEFVGRMPVVVSLAALSRDDLVKVLTEPKNAVVRQFAKFL
                     SLDRVELVFTDDALEATARLATERKTGARALRTIVEEALLEVMYDIPERTDVTKVVIT
                     ADTIEHGRPPMLVTETDETAPRRRGGRARDGATREDTTREESA"
     gene            563..1678
                     /locus_tag="VGO32_02630"
     CDS             563..1678
                     /locus_tag="VGO32_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199679.1"
                     /translation="MTATQRRSGFLGKWTADDQPPEEEQRAETDMPEPADTSTVEQTV
                     DPIAGVEPGVEPSDQFLSSLTAAMRRVADDARESSLADARAAIAAKIAAMRATATARS
                     DELRTRAEADVTGIGEWSRAEIVRIEAETQRKVEQRRAQLAEQLDEHDRRSSADIAAL
                     ESQVEQYEGELALFFSQLGEITDPDTFISAARRMPRLPQPEAEPEADAAVAGDASPAE
                     HEDRLRTLGIDRNGDTDMAEGDAAPTNGTHADADGAPSTADAEAPVTPDDPTPAQPFA
                     EPPPIVADATLDTTTTIAAVGLGSFGAVTAFKSALEKAEGVTAVRLSLGSGGEFVYTV
                     THRADLDLGDVSAAALPGATVERSADGVLQLSAGKHR"
     gene            1938..4634
                     /locus_tag="VGO32_02635"
     CDS             1938..4634
                     /locus_tag="VGO32_02635"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV7199680.1"
                     /translation="MADKDTSLASRYAPTSVEPRVYERWLASGAFTPAEEPTPGAVRF
                     VITMPPPNVTGSLHTGHALFVTAEDILIRYHRMRGDDTLWVPGVDHASIAAQFVLDKL
                     LAVEGLDRASLGREAYLDRMWAFMDETRDRISLQLRRLGASADWSRNRFTMDEGSARA
                     VRVAFKRLSDAGYVYRGEALGNWCPRCRTTISDLENIHRDEVGTLWTIRYHLVGGDGA
                     PDPEHWISVATTRPETLLGDMAVAVHPEDERYRDLVGREAVLPFLGRRLPIIADTAVD
                     PTFGTGAVKITPAHDPDDYETGKRHGLTPITILDEEARINEAGGEFAGLDRFDARQRI
                     VERLTALGDLEGEVAHPMVVGHCERCGTVIEPRLSVQWFIRTADLAARALASVREGRT
                     RIMPPRFEKVYAHWMENIRDWAVGRQLWWGHRIPAWFCPDGHFTISDEADGPSACETC
                     GRTATELTQETDIFDTWFSSGLWPFSTLGWPDDTPDLRRFYPTSVMETGYDILFFWVA
                     RMMMLGLFLTDAEPFRTVFLHGLVRAEGGVKMSKTKGNVTDPVELIDEIGADAVRLAL
                     TSGTSAGNDQRLTMAKLDGARNFANKLWNAARFVLGSEPIGDSAEEQSGPAGASMPER
                     WIRSRLAAITADTTQRLDRLDLGGYAAAATEFAWNDFCDWYLELAKIEMRDPGASSAA
                     RSRSWQTAVDVLADTLRLLHPVMPFVTEEIWSAIAPARQGAGAAEPDLLMTSPWPTAG
                     PRDAAAEAEFTSLAEVIRTTRTLRTEAGIPAGAIVELHIAAASTTARAALEDGVRYLE
                     PMARARTELLPADAPIPDGRGAATALGAVWLGDASEVAPTAAAGTDREAELRVQRDRV
                     RALLADAAFLERAPAAVVDRERVRLADLEERLRQIGTGDGSG"
     gene            4670..5392
                     /locus_tag="VGO32_02640"
     CDS             4670..5392
                     /locus_tag="VGO32_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199681.1"
                     /translation="MGFLRRNKPEPAPEPAPQVFAQVGGRQYSVALAYLARSGDAIRL
                     APGPNVVTSLPRIVEPLTLDGIEVVAPLPLELSSATPAVERLPELEQWILARRQAGPV
                     TRHALHVLEQTEAVDMTIDTLIVGLLHGETDTTGYPTFDAISGGLASHFDDLTGELIV
                     RAVLGWGGKGQRGDTERIGSRILSNLQQQVLVSGLQVSTADTPVHRDAGEGKVCGHCG
                     FDARGTGAFYCPKCGMKLGGAG"
     gene            5498..6763
                     /locus_tag="VGO32_02645"
     CDS             5498..6763
                     /locus_tag="VGO32_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7199682.1"
                     /translation="MERPRVLLAAHDDAAEIAQALVRAGFEVIGSEEAFEATIVEGHP
                     PEFAIIDADLAPSIVRRVHRRLNENGLVPTLILLGDEAGELDDVWQVSDEFVVKPVLP
                     DSLVYRLQAFLIRAGSPGTPSTTPGPTTDTGTEPGTEAEIPVMIGAHDPASARVIAVF
                     APKGGVGKTTVSVNLSVALRMQTRQRVLLLDADAGVGNVTAVIDVPAKRGLADMADSP
                     PELWTDDAFRSLIVNHEPSGLDVLTWGFEPADAERIGPDLMIAAVRWARQHYELVIVD
                     THPGYDDRTVAMLSVANEILLTVTPEVGPLRNSAQFLQLAHELGIDDTIRVVANRANH
                     GVAIRDMESALGRPVIATIVSAGATAVMAANAGTPLVIRFPKERITADIHRLARIVAG
                     TEEAASVIPSDKRRWSFATILRAGSATTN"
     gene            6831..7247
                     /locus_tag="VGO32_02650"
     CDS             6831..7247
                     /locus_tag="VGO32_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEV7199683.1"
                     /translation="MPTYDYRCRDCGHTIEIMHSILEDGPDACERCRGPVERVLHPTG
                     VIFRGSGFYVTDSRAGNTSSGGSSDGGGGSGSSKSGSGSKDASSDGGGGSGSGSKDAS
                     SDGGSGTGSGAKDSGAGAKDAGSGAKDSGPATGGSD"
     gene            complement(7249..8424)
                     /locus_tag="VGO32_02655"
     CDS             complement(7249..8424)
                     /locus_tag="VGO32_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199684.1"
                     /translation="MTELLTESFCERCGNRFELGTSEPMTTKQKTRGLVTGLRQFIMS
                     TDSLEDSIGDAMRAEEQTLAARQIDAFQETFNFCLDCRRYTCVSCWNDDAGRCRSCMP
                     VAGIDDVAGLDERLAGPALMPAPADGTPLLTTAAEPAWPAEEELPAPPLAWPSADEIP
                     PAVAAEAVEPTVEEPELEVAAEVEIPEPQVVPGFAAEEPALEVAAEVEIPEPETVAEP
                     EIVPEPQVVPGFAAEEPEAVEEPLDEPIAAAAELEPETPEPTPAEPPVRAPRRDLRDT
                     FVRGPLVPRPAPSAEPIPESLAARRSQLDALGLDDHGEGAVSVGQRDALPYRSSGAGS
                     AAGSAALAAVWDASTRALEAGAGPQRASIRPCGACGLTVSSTARFCRRCGAPQTLSA"
     gene            8540..>8739
                     /locus_tag="VGO32_02660"
     CDS             8540..>8739
                     /locus_tag="VGO32_02660"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEV7199685.1"
                     /translation="MGIFRRLEDFLERVVEAPAGRMGAAPQPVTLAKRIARAMDTNKR
                     FGEDGVIVPNRYVVHLSPTDYAS"
BASE COUNT         1367 a         3123 c         3002 g         1247 t
ORIGIN      
        1 tacgggctca ttcccgagtt cgtgggtcgg atgccggtcg tggtcagcct ggccgccctg
       61 tcgcgcgacg acctggtcaa ggtcctgacc gagcccaaga acgcggtcgt acgccagttc
      121 gcgaagttcc tgtcgctcga ccgggtagag ctcgtcttca ccgatgacgc gcttgaggcc
      181 accgcacggc tggccaccga gcgcaagacc ggtgcccggg cgctgcgcac gatcgtcgag
      241 gaggcgctgc tcgaggtcat gtatgacatc ccagagcgga ccgacgtgac caaggtggtc
      301 atcacggcgg acacgatcga gcacggccga ccaccgatgc tggtcaccga aaccgacgag
      361 accgcgccac gccgccgcgg tggccgggcc cgcgatgggg ccacccgcga ggacaccacc
      421 cgagaagagt cggcctgacc gattcggccg cgcggtggta ccaccgttcg gtctgcccgg
      481 taccaccggg cgggcgggga tgccggaggc ccggggctac gatgtccccg caccgcattc
      541 aactgaccaa cggagacggg tcatgacggc aacccagcgt cgctcagggt tcctcggaaa
      601 atggaccgcc gacgaccagc cgccagagga agagcagcga gccgaaacgg acatgcctga
      661 gcctgccgac accagcaccg tggagcagac ggtggacccg atcgccggtg tggaaccggg
      721 ggtcgagccc tccgaccagt tcctcagctc cctgactgcc gccatgcggc gggtggcaga
      781 cgatgcccgc gagtcgagcc tggccgacgc ccgcgccgcg atcgcagcca agattgccgc
      841 catgcgggcc accgccaccg cccggtccga cgagctgcgg acccgcgccg aggcggacgt
      901 caccggtatc ggcgagtggt cgcgagccga gatcgtccgc atcgaggccg agacccagcg
      961 caaggttgag cagcgccgcg cgcagctggc cgagcagctc gacgaacacg accgccggtc
     1021 gtcggccgat atcgccgcgc tcgagtcgca ggtcgagcag tacgagggtg agctcgccct
     1081 gttcttctcg cagctcggtg agatcaccga tccggatacg ttcatctccg ccgctcgtcg
     1141 catgccgcgc ctgccccagc ccgaggccga gcccgaggcc gatgccgcgg tcgccggcga
     1201 cgcgtcaccc gccgagcacg aggaccggct ccggaccctc ggcatcgacc gcaacggcga
     1261 taccgacatg gccgagggcg acgccgctcc gaccaacggc acccacgccg atgccgacgg
     1321 cgcgccgtcg actgccgatg ccgaagcccc cgtcacgccc gacgacccga cccctgctca
     1381 gccgttcgcc gaaccgccgc cgatcgtggc cgatgccacc ctcgacacca cgaccaccat
     1441 cgccgccgtc ggcctgggca gcttcggcgc ggtgaccgcg ttcaagtccg cgctcgaaaa
     1501 ggcggaaggc gtcaccgccg tccggctgag cctcggatcg ggcggggagt tcgtctacac
     1561 cgtgacccat cgtgccgatc tggacctcgg agacgtgtcc gccgccgcgt tgccgggcgc
     1621 gaccgtcgag cgcagcgccg acggcgtgct ccaactcagc gcgggcaagc accgctgaca
     1681 tcaaccaggg ggtagactcc gctcgacggc accgaccgtg aggagtaccc ctccgacggc
     1741 cgacagagac gcgggtcacg gctgagagcc cgctcggcaa gcgagggcga aggtcgcacg
     1801 cgagccgtcc gggattcgcc cgatagcgcg aaaccgagga tgtcgtcggc gcgccgggga
     1861 catcgaacga aggtggtacc acgggcccgc tcgtccttcg acgagcgggc tggttgtttt
     1921 tcacggagct ctgggccatg gcagacaagg acacatcgct cgcgtcccgc tacgcgccga
     1981 catcggtcga gccgcgcgtc tatgagcgct ggctggcgag cggtgcattc acccccgctg
     2041 aagagccaac gcctggggct gttcggttcg tgatcaccat gcccccgccc aacgtcacgg
     2101 gctcgctcca taccggccat gccctgttcg tgacggccga ggacatcctg atccgctacc
     2161 accgcatgcg cggagacgac acgctgtggg tgccgggcgt cgaccacgcc tcgattgccg
     2221 cccagttcgt gctcgacaag ctgctggccg tcgagggcct ggaccgggcg tcgctcggcc
     2281 gcgaggcgta cctggaccgg atgtgggcct tcatggacga gacccgcgac cgaatctcgc
     2341 tccagctccg ccggctcggc gcgagcgccg actggagccg aaaccggttc acaatggacg
     2401 agggaagcgc ccgcgcggtc cgcgtcgcat tcaagcgtct ttcggacgcg ggctacgtgt
     2461 accgcggcga ggccctcggc aactggtgtc cgcgctgccg gaccacgatc agcgacctgg
     2521 agaacatcca ccgggacgag gtcgggacgc tgtggacgat ccgctaccac ctcgtcggcg
     2581 gcgacggggc tccggacccc gagcactgga tctcggtggc cacgacccga cccgaaacgc
     2641 tcctcgggga catggcggtg gcggtccacc ccgaggacga gcgctaccgg gacctggtcg
     2701 gccgcgaggc cgtcctgccg ttcctcggcc gccggctgcc gatcatcgcc gacacggccg
     2761 tggatccgac cttcggcacc ggcgccgtga agatcacgcc ggcccacgat ccagacgact
     2821 acgagacggg caagcgacac ggcctgacgc cgatcacgat cctggacgag gaggcgcgga
     2881 tcaacgaggc cggcggcgag ttcgcgggcc tcgatcggtt cgacgcccgt cagcggatcg
     2941 tcgagcggct gaccgcgctc ggcgacctgg agggagaggt cgcgcacccg atggtggtcg
     3001 gccactgcga gcggtgcggg accgtgatcg agccacggct ctcggtccag tggtttatcc
     3061 ggaccgcgga cctggcggcg cgcgcactgg catcggttcg ggaagggcga acccggatca
     3121 tgccgccgcg gttcgagaag gtgtatgccc actggatgga gaacatccgc gactgggcgg
     3181 tcggccggca gctgtggtgg ggccatcgga ttccggcctg gttctgtccc gacggccatt
     3241 tcacgatcag cgacgaggcc gacgggccga gcgcctgcga gacctgcgga cggaccgcga
     3301 ccgagctgac ccaggagacc gacatcttcg acacctggtt cagcagcggg ctgtggccct
     3361 tctccaccct tggctggccg gacgacaccc cggacttgcg tcgcttctac ccgacctcgg
     3421 tgatggagac cggctacgac atcctcttct tctgggtcgc ccggatgatg atgctcggcc
     3481 tgttcctgac cgatgccgag ccgttccgca ccgtcttcct ccatggcctc gtccgtgccg
     3541 agggcggcgt gaagatgagc aagacgaaag gcaacgtgac cgatcccgtc gagctcatcg
     3601 acgaaatcgg cgccgacgcg gtgcgcctcg cgctgaccag cgggacctcg gccgggaacg
     3661 accagcggct gaccatggcc aagctcgacg gtgcccggaa cttcgccaac aagctgtgga
     3721 acgccgcgcg gttcgtgctc ggcagcgagc cgatcgggga ttccgcagag gagcagtcag
     3781 gacccgccgg tgcgtccatg cccgaacgct ggattcggtc tcgtctggcc gcgatcacgg
     3841 ctgacaccac acagcgtctg gaccggctcg atctcggcgg ctatgccgcg gccgcgaccg
     3901 aattcgcctg gaacgacttc tgcgactggt acctcgagct ggccaagatc gagatgcgtg
     3961 acccgggtgc ctcttccgcg gcccggtcga ggagctggca gacggcggtc gacgtcctcg
     4021 ccgacacgct tcggctcctc catccggtga tgcccttcgt gaccgaggag atctggtcgg
     4081 caatcgctcc cgcgcggcag ggggcgggcg ccgccgaacc ggatctgctc atgaccagcc
     4141 cgtggccaac ggcgggaccc cgggacgccg cagccgaagc ggagttcacc tcgctggccg
     4201 aggtgatccg gaccacccga accctgcgca ccgaggctgg aatcccggcc ggagcgatcg
     4261 tcgaactcca catcgccgcc gccagcacga cggcccgcgc cgcgcttgag gacggcgtgc
     4321 gatatctcga gccgatggcc cgcgcccgaa ccgagttgct gccggccgac gcgccgattc
     4381 ccgacggacg cggcgccgcc accgccctgg gcgccgtgtg gctgggcgac gcctccgaag
     4441 tggcccccac ggcggctgcg ggcaccgatc gcgaagcgga gctgcgggtc cagcgcgacc
     4501 gggttcgggc tctgctggcc gacgcggcct tcctggagcg tgccccggcg gcggtcgtcg
     4561 accgtgagcg ggtgcggctg gccgacctcg aggagcgtct gcgccagatc gggactggcg
     4621 acgggtcggg gtagaatcgc caccgatcac gcccgcagaa gaggtccgcg tgggattcct
     4681 ccgtcgcaac aagcccgagc cagcccccga gccagccccg caggtcttcg cccaggtcgg
     4741 cggacgtcag tactcggtcg ccctggccta cctcgcgcgg agcggtgacg cgatccggct
     4801 ggcacccggt ccgaatgtcg tcacctcgct gccgcggatc gttgagccgc tcaccctgga
     4861 cgggatcgag gtcgtcgcgc cgctccccct cgagctttcc tcggcgactc cggccgtcga
     4921 gcgcctgccc gagctggagc agtggatcct tgcccgccgt caggcaggac cggtgactcg
     4981 ccacgccctc catgtcctcg agcagaccga ggcggtggac atgacgatcg acaccctgat
     5041 cgtcggcctc cttcacgggg agaccgatac gaccggctat ccaaccttcg acgcgatcag
     5101 cggcggcctc gccagccact tcgatgacct gaccggcgaa ctcatcgtgc gggccgtcct
     5161 cggctggggc ggaaagggcc agcgagggga taccgagcgc atcggctcgc ggatcctcag
     5221 caacctccag cagcaggtcc tcgtctcggg gctgcaggtc agcaccgctg acacccccgt
     5281 ccatcgcgac gcgggcgagg gcaaggtctg cgggcactgc ggcttcgatg cccgcggcac
     5341 tggcgcgttc tactgcccga agtgcgggat gaagctcggc ggcgccggct agtccggccc
     5401 gaaacgaacc ggaggcggcc tcggtacctt agtcccattg ttcgcgtgag cgagcgccgt
     5461 cgacactggt gccccgcacc gcaccaaaag gcacctgatg gaacgacccc gcgtcctgct
     5521 tgctgcacat gacgatgctg ccgagattgc ccaggccctg gttcgtgccg ggttcgaggt
     5581 catcggcagc gaagaggcct tcgaagcgac gatcgtggag ggccacccgc ccgagttcgc
     5641 catcatcgac gccgacctcg cgcccagcat cgtccggcgg gtccatcgcc gcctgaacga
     5701 gaacggcctc gtgccgaccc tcatcctcct cggcgacgag gctggcgagc tggacgacgt
     5761 gtggcaggtc tcggacgagt tcgtggtcaa gcccgtcctg ccggattccc tggtgtatcg
     5821 gcttcaggcc ttcctcatcc gtgccgggtc gccggggacc ccgtccacga ccccgggccc
     5881 gaccacggac accgggaccg agcctgggac ggaggccgag atcccggtga tgatcggtgc
     5941 tcacgacccc gccagcgccc gcgtgatcgc cgtcttcgcg ccgaagggcg gcgtggggaa
     6001 gacaacggtc tccgtcaacc tgtccgtcgc ccttcggatg cagacccggc agcgtgttct
     6061 gctcctggac gccgacgcag gcgtcggcaa cgtgactgcg gtcatcgacg tccccgccaa
     6121 gcgaggtctg gccgacatgg ccgacagccc tcctgaactc tggaccgatg atgcattccg
     6181 gtcgctgatc gtgaatcacg agcccagcgg cctggacgtc ctgacctggg gcttcgagcc
     6241 ggccgatgcc gagcgcatcg gccccgacct catgatcgcc gccgtccgct gggcccgcca
     6301 gcactatgaa ctggtcatcg tcgacaccca tcccgggtac gacgaccgca ccgtcgccat
     6361 gctgtcggtc gccaacgaga tcctgctcac cgtcaccccg gaggtcgggc cgctgcgcaa
     6421 cagcgcccag ttcctccagc tcgctcatga gctcgggatc gacgacacga tccgcgtcgt
     6481 tgccaaccgc gcgaaccatg gggtggccat ccgcgacatg gaatccgccc tgggacgccc
     6541 ggtcatcgcg accatcgtgt ccgctggtgc caccgcggtc atggctgcta acgccggcac
     6601 gccgctcgtg atccgcttcc cgaaagagcg gatcaccgcc gacatccatc gcctggcccg
     6661 catcgtggcg ggcacggaag aggccgcgtc ggtcatcccg tccgacaagc gccgctggtc
     6721 gttcgccacc atccttcggg ccgggagcgc gacgaccaac tgaccacgcg gccccggaac
     6781 ggccgggtcg cgttgccgac cggcagcccg tcctgccatg atcccgcgac gtgccgacct
     6841 acgactaccg ctgccgggac tgcggccaca cgatcgagat catgcattcc atcctcgagg
     6901 acggacccga cgcgtgcgag cgctgtcgcg gtccggtcga acgggtcctc cacccgacgg
     6961 gggtgatctt ccgcgggagc ggcttctacg tcaccgattc ccgtgccggc aatacgtcca
     7021 gcggtgggag ctcggacggc ggtggtggct cgggcagctc gaaatcgggc tctggctcga
     7081 aagacgcgag ctcggacggc ggtggtggct cgggctctgg ctcaaaagac gcgagctcgg
     7141 acggcggcag tggcacggga tcgggcgcga aggactcggg cgccggcgcg aaagacgcgg
     7201 gctcgggcgc gaaggactcg ggccccgcga ccgggggcag cgactaggtc aggcgctcag
     7261 cgtctgaggt gcgccgcatc ggcggcagaa tcgggcggtc gaggatacgg tcaggccgca
     7321 ggcaccacag ggacgaattg aggcccgctg cgggccggct cccgcttcga gcgcacgagt
     7381 cgatgcgtcc cacacggccg cgagggcggc actgccggcg gcggagccgg caccactgga
     7441 tcgatatgga agcgcgtcgc gctgaccgac cgacacggcc ccctcgccat ggtcgtccag
     7501 gccgagggcg tcgagctggc tgcggcgcgc ggcgaggctc tcggggattg gttcggcgga
     7561 cggggccggg cgcggcacca gcggcccgcg gacgaacgtg tcccgcaggt cccggcgcgg
     7621 tgcgcggacc ggcggctcgg cgggtgtggg ctcgggggtc tccggctcga gctcggcagc
     7681 ggcggcaatc ggctcgtcga gcggctcttc gacggcctcc ggctcctcgg cggcgaatcc
     7741 gggcacgacc tggggctcgg gaacgatctc cggctcggca acggtctccg gttcgggaat
     7801 ctcgacctcg gccgccactt ccagcgccgg ctcctcggcg gcgaatccgg ggacgacctg
     7861 gggttcggga atctcgacct cggccgccac ttccagctcc ggctcctcga ccgtgggctc
     7921 caccgcctcg gccgcgaccg caggcgggat ctcgtcggcg ctcggccagg cgagcggggg
     7981 agcagggagc tcctcttccg cgggccatgc tggctctgcg gcggtggtca ggagcggtgt
     8041 cccgtccgcg ggagccggca tcaaagccgg cccggccaga cgctcgtcca ggcccgcgac
     8101 gtcgtcgatg ccggcgaccg gcatgcacga gcgacatcgg cctgcgtcgt cgttccagca
     8161 gctgacgcac gtgtaccgcc ggcagtccag gcagaagttg aacgtttcct ggaacgcatc
     8221 gatctggcgg gcggcgaggg tctgttcctc ggcccgcatg gcgtccccga tcgagtcctc
     8281 caggctatcg gtgctcatga tgaactggcg gaggccagtc accaggccac gcgtcttctg
     8341 cttcgtcgtc attggctcgg acgtgccgag ctcgaaacgg ttcccgcagc gctcgcagaa
     8401 ggattcggtc agcagttcag tcatggtcag gacccgttcg gaacgggcgg ccagtatagc
     8461 cacgaggccc gggcttcccg gatcgggcca aagtaccgcg aagggcgccg ttcgtacccc
     8521 gtgctagcat cgctggccga tgggcatctt ccggcggctc gaggacttcc tcgagcgagt
     8581 ggtcgaggcg cctgccggac gcatgggagc ggcaccgcag ccggtgaccc tggccaagcg
     8641 gatcgcgcgc gccatggaca cgaacaagcg attcggcgag gacggcgtga tcgtcccgaa
     8701 tcgctatgtc gtccatctca gccccaccga ctacgcctc
//
LOCUS       DASYGU010000100         8090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1816923, whole genome shotgun sequence.
ACCESSION   DASYGU010000100 DASYGU010000000
VERSION     DASYGU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816923"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VGO32_02665"
     CDS             <1..483
                     /locus_tag="VGO32_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7199686.1"
                     /translation="LPFFIYVFTRRSGYPLPVSVITAVQDRASNLAGLKVSESPMADV
                     APYLELGLPVLVGSEPLIVPALAAGADGTVSGLAAAFPDEVRAVLDAPGPSGEARLEV
                     LRGVMEAQPFVPSIKHVLGRRGVPVRADVRAPLRPLDPARVGVLDRALAELEPSAVRG
                     "
     gene            complement(466..1647)
                     /locus_tag="VGO32_02670"
     CDS             complement(466..1647)
                     /locus_tag="VGO32_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199687.1"
                     /translation="MTPAVILATTLFAIGVDNYIVVAILPEIAHEFGQPVENVGLLAS
                     AYAIPLALLAIPFGPVSDRLGRRFSIVLGMAIFLGAVVASGLAPSYPLLLAARIVNGF
                     GAAIVGPAVFAYAADLSSTEQRGRAMATIVAAYPVSTILGLPLGGLIAATFGWRAVFA
                     FIALVAVAALALVWRLPRDAPRAAAAETYLAGLRALVASRPTLAAVTVTLVWFMGSFG
                     LFVYLGTYFTESFGFSSLQVGLTLTIIGFVGLFATRVAARMIGRLGAKRVVMIGLACF
                     SVAAFLLPLTRVFLPLALLNMALWVFGVWFGLPAQQAIVSETLPAWRGTALAANTSAL
                     YLGGAIGPAMTGAILAVGGFAVAGPWSAAIGLMALVLAGLLLPSVVGPRTAVNPSAAD
                     G"
     gene            complement(1661..2167)
                     /locus_tag="VGO32_02675"
     CDS             complement(1661..2167)
                     /locus_tag="VGO32_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7199688.1"
                     /translation="MSNYVEIHGDGWRFQPRVAAVCVWEDHVLLQGALDGAFWVLPGG
                     RLLPLELTADALVRTMRWEIGQEVAVRRLLWVMEYVTPMGGQPVHEMGFYYAVDLPAN
                     SPLFDLTHDHAGVERGHDLVLRWSPVDALSDIPLFPEFLRTALRQMPDGPQHIVQVTP
                     SEAGLRPA"
     gene            complement(2179..3276)
                     /locus_tag="VGO32_02680"
     CDS             complement(2179..3276)
                     /locus_tag="VGO32_02680"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEV7199689.1"
                     /translation="MSLDVATIRSAPKVLLHDHLDGGLRPRTVIELAAETGYRDLPTT
                     DPDALAEWFYQGKPDSSLELYLEAFAHTVGVMQTPEALERVAAECVEDLAADGVVYAE
                     IRFAPELHLEKGMTLDGVVESVLAGVRRGAEGRSISIGILLSAMRQAARSVEIAELSI
                     RHRDRGVLGFDMAGPEAGYPPTRYLDAFHLIAHENFHFTLHAGEAFGLPSIWEALQWC
                     GAERLGHGVRIVDDITVHPDGHVALGRLAAYVRDTRVPLEMCPTSNVHTGAVRSLADH
                     PIDLLRRLRFRVTVNTDNRLMSGISLTDEFAALSRTFGIGLDEMEWLTLNGIKSAFLP
                     FDGRIRIINEQIKPGFARLRAEAAAAELATG"
     gene            complement(3273..3638)
                     /locus_tag="VGO32_02685"
     CDS             complement(3273..3638)
                     /locus_tag="VGO32_02685"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV7199690.1"
                     /translation="MGHSPEVDAWLAAYANPQKPVVARVREIILDADPRIGEVIKWST
                     PTFVYRGNLASFQPRATKFVSLLFHEGASIPGTHPRLDGGGDHARYMRIADLADADYA
                     AAELMAIIRAWCDARDAAG"
     gene            complement(3643..4266)
                     /locus_tag="VGO32_02690"
     CDS             complement(3643..4266)
                     /locus_tag="VGO32_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEV7199691.1"
                     /translation="MATRIALDGVGGQGVRLIAGILGSLLARMGKQVTVLLDYDSSVR
                     GSMSDAFVIFDDVPIDNPVVEEADILLRLADRGHLRISGRKVVTDLGLGLPGDEQIPF
                     ASLGSAHFGKELFGNMIALGRLLKLAGIEYDEGAIRDSLPRRYQDENVAAIRFGYDLT
                     EDEIARLAAAAPPSRFEMNDAATAPFHRRTGIHSGESITDATPAETG"
     gene            complement(4283..5317)
                     /locus_tag="VGO32_02695"
     CDS             complement(4283..5317)
                     /locus_tag="VGO32_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7199692.1"
                     /translation="MPEAAFAEMGFKSKLKMDLFPTIWCPGCGIGTIMIELAMVLDEL
                     GLDQSNTVIVTGIGCTGRMGSYMKYDAVYTLHGRTLPVAEAIKTVRPELTVIVVAGDG
                     DTASIGGNHLIHAIRRNARVTILCNNNEIYGLTGGQTGPTTPNGTKTLSSPGGNPNTP
                     INLQGIVRSSPHALYARTTVYHQPQMRTAIREAIQHDGFSFVDITSQCIENNGRRIGF
                     ANANEMLTFYRKTYKRAPKGAERLGPHELGILYPEGHAEHSNGALADEPAQTDGPAPI
                     NGAGATPAPRAERPVVSDDEKARKRAEAQKRLALMQRLDPAVKLQVAKGELTLPEALK
                     AAGIDPAELE"
     gene            complement(5333..6466)
                     /locus_tag="VGO32_02700"
     CDS             complement(5333..6466)
                     /locus_tag="VGO32_02700"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV7199693.1"
                     /translation="MRRLVQGNEAVFRGAIAAGASYYAGYPISPSTEILNIASGWAAE
                     HPEFGFLQAEDEIASANSVIGASLAGAKSFTATSGPGFSLMQEAIGYAQKVGVPSVFV
                     DVQRVGPATGMPTMPGQGDILQVRYGSSGDYTPIAFYPNSVPEAFNLAVAAFNAAEES
                     RSPVVLLSDTFVAHLNEVVDLEELEAGVSIIPRDLEPLASGGADPRFFAGVLMYEHGP
                     NAGEPATADADEYLRQYYAAKHRHREVADRYALYQHGWNTDAKTLVIGYGIVSRVAAP
                     LAEEYALFRPIRIHPMLTEELRAVAEGYETIVAVEANDGQYADMAELALRREVKRVPL
                     LGGRISLEAVNAGLARVLGDGPDEPPVPPAPNQRADRAPLGIG"
     gene            complement(6471..6827)
                     /locus_tag="VGO32_02705"
     CDS             complement(6471..6827)
                     /locus_tag="VGO32_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEV7199694.1"
                     /translation="MSADRLANALHFGTGTSTGHQDGVRWIDYQNISWNPVFCKRCDI
                     CVDICPKGTLVLRDDAILEEDNCILCGLCERYCPDLAIEMIPAAIAAYAAKGDAGAGD
                     AGTGNAVRADGPGDEG"
     gene            complement(6913..7878)
                     /locus_tag="VGO32_02710"
     CDS             complement(6913..7878)
                     /locus_tag="VGO32_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7199695.1"
                     /translation="MVPNASGAAGWKVAAALGIVYVVWGSTYLGIAVMVETLPPLVAA
                     GVRYTTAGVILLAALIVWSRLRGGRLERPTRQHWRSALLIGSLLLVGGNGGVVLGEQL
                     IATGIAALIIATTPIWMAIFEAVVAGRRPSRLAVAGLVAGLIGVAILVVPLEGSPPID
                     PLGLALVAGAAISWSIGSVYSRRIPMPTSPFQGAGLQMLAGGAVMLLIGLLRGELADV
                     NPDAFSTESLIALAYLILFGSLVAFTAYFWLLSHVSISVISTTAYVNPIVAVALGVIV
                     LSEPMTPRTWLAAAIIMGAVVAMVTGRPRTASEPEPAVEPEPNPA"
BASE COUNT         1265 a         2779 c         2850 g         1196 t
ORIGIN      
        1 ctcccgttct tcatctacgt cttcacccgc cgaagcggct acccgctgcc cgtgtcggtc
       61 atcaccgccg tgcaggaccg cgcttccaac ctggctgggc tgaaggtgtc ggagtcaccc
      121 atggccgacg tcgcgcccta cctggagctg ggactaccgg tgctcgtcgg atcggagccg
      181 ttgatcgtcc ccgccctggc cgctggcgcc gacggcacgg tcagcgggct ggcggccgcg
      241 ttcccggacg aggttcgggc ggtcctcgac gcacccgggc cgtcgggcga ggcacgcctc
      301 gaggtcctgc gcggggtgat ggaggcacag ccgttcgtcc cgtcgatcaa gcacgtcctc
      361 ggccgccgag gcgtgcccgt ccgcgccgat gtgcgtgccc cgctccgccc gctggatccc
      421 gcgcgggtcg gggtgctcga ccgcgcgctt gccgagctcg aaccctcagc cgtccgcggc
      481 tgacggattg accgctgtcc gtgggcccac cacggacggc aggagcaggc cggccagcac
      541 gagggccata aggccgatcg ccgcgctcca ggggccggcc accgcgaatc ctccgacggc
      601 caggatcgcg ccggtcatgg ccggaccgat cgcgccgccg aggtacaggg cgctcgtgtt
      661 cgccgcgagc gccgttccgc gccacgcggg gagggtttcg ctcacgatcg cctgctgggc
      721 gggcagtccg aaccagacgc cgaacaccca cagggccatg ttgaggagcg cgagcgggag
      781 gaagacccgg gtcaggggca ggagaaacgc ggccaccgag aagcaggcga gcccgatcat
      841 gaccacgcgc ttggcgccga gccggccgat catccgcgca gccacccggg tcgcgaacag
      901 gccgacgaag ccgatgatcg tcagggtcaa gccaacctgg agcgagctga agccgaacga
      961 ctcggtgaaa tacgtgccca ggtagacgaa cagtccgaag gaccccatga accacacgag
     1021 ggtcacggtc accgccgcca gggtcgggcg gctcgcaacc aaagcacgca gcccggccag
     1081 gtaggtttcg gcggcggccg cgcgtggggc atcgcggggc aggcgccata cgagggccag
     1141 cgccgcgacc gcgacgagcg cgatgaaggc gaaaaccgcg cgccacccga acgtggccgc
     1201 gatcaggccg ccgaggggca gacccaggat ggtcgagacc gggtaggccg ccacgatcgt
     1261 cgccatcgcc cgcccacgct gctcggtcga ggacaggtcc gccgcgtacg cgaacaccgc
     1321 cgggccgaca atggccgcgc cgaacccgtt gacgatccgg gccgccagga ggagtgggta
     1381 ggacggcgcc aggccggagg ccacgaccgc gccgaggaag atcgccatgc ccagcacgat
     1441 cgagaaccgg cggccgaggc ggtcgctgac cggcccgaac gggatggcca gcagcgcaag
     1501 cgggatcgcg tacgcgctgg cgaggagtcc gacgttctcg accggctgac cgaactcgtg
     1561 cgcgatctcg ggcaggatgg cgacgacgat gtagttgtcg accccgatcg cgaacagggt
     1621 cgtggccagg atgacggcgg gcgtcactcc tcgggcggcc tcaggccggc ctcaggccgg
     1681 cctcagaggg cgtcacctgg acgatgtgct gggggccgtc cggcatctgc cgcagcgccg
     1741 tccgcaggaa ttcggggaaa agcggtatgt ccgacaaggc atcgaccggg gaccagcgga
     1801 ggaccaggtc gtggccgcgt tccaccccgg cgtggtcgtg cgtcaggtcg aacagcgggc
     1861 tgttcgcggg caggtcgacg gcgtagtaga aacccatttc gtggaccggt tgacctccca
     1921 tcggggtcac atactccatc acccacagta gccgccggac tgccacctcc tgcccgatct
     1981 cccagcgcat ggtccgaacc agcgcgtcag cggtcagctc cagcggcagc aggcgcccgc
     2041 cgggcagcac ccagaaggcg ccgtcgagcg ccccctggag caggacgtgg tcctcccaga
     2101 cgcagacggc tgctacccgt ggctgaaagc gccacccgtc gccgtggatt tcgacgtagt
     2161 tcgacaagaa gcttgaggtc agccggtcgc cagctcggcg gccgcagcct cggcgcggag
     2221 gcgggcgaag ccgggcttga tctgctcgtt gatgatccgg attcggccat cgaacggcag
     2281 gaacgcgctc ttgatgccgt tcagggtcag ccactccatc tcgtccaggc cgatgccaaa
     2341 cgtccgcgac agcgcggcga actcatcggt cagggagatg ccgctcatca ggcggttgtc
     2401 ggtgttcacc gtcacccgga acctaagccg ccggaggagg tcgatcgggt ggtccgcgag
     2461 cgagcggacg gccccggtat ggacgttcga ggtcgggcac atctcgagcg ggacccgtgt
     2521 gtcacggacg tacgccgcca gccgccccag cgccacgtgg ccgtccggat gcacggtgat
     2581 gtcgtccacg atccgaaccc cgtggcccag ccgttcggcg ccgcaccact ggagggcctc
     2641 ccagatcgag ggcaggccga acgcttcgcc ggcgtggagc gtgaagtgga agttctcgtg
     2701 ggcaatgagg tgaaacgcgt cgaggtatcg ggtcggcggg tagccggcct ccggcccggc
     2761 catgtcgaag cccagcacgc cccggtcacg gtgccgaatg gacagctcgg caatctcgac
     2821 cgatcgggcc gcctggcgca tcgcgctgag caggatgccg atcgagatgc tccgtccctc
     2881 ggcgccgcgc cggaccccgg ccaggaccga ttcgaccacg ccatcgaggg tcatcccctt
     2941 ctcgaggtgg agctccggcg cgaaccggat ctcggcgtag acgaccccat cggccgcgag
     3001 atcctcgacg cactcggcgg cgacccgctc gagcgcctcg ggcgtctgca tgacgcccac
     3061 cgtgtgggca aaggcctcga gatagagctc gaggctcgag tcgggcttgc cctggtagaa
     3121 ccactcggcc agggcgtcgg ggtcggtggt gggcaggtcc cgatagcccg tctccgccgc
     3181 aagctcgatc acggtacgag gccggaggcc tccatccagg tggtcgtgga ggagcacttt
     3241 gggcgccgac cggatggtgg cgacgtcgag gctcatccgg cggcgtctcg ggcgtcgcac
     3301 caggctcgga tgatcgccat cagctcggcc gccgcgtaat ccgcatcggc gagatcggca
     3361 atccgcatgt aacgagcgtg gtcaccacca ccatcaagtc ggggatgggt gcccggaatc
     3421 gaggcgccct cgtggaacag gaggctgacg aatttcgtcg cccgcggctg gaagctggcg
     3481 agattgccgc ggtacacgaa ggtcggcgtg ctccacttga tgacctcacc gatccgcggg
     3541 tcggcgtcga ggatgatctc ccgaacgcgg gcgacgaccg gcttctgcgg gttggcgtac
     3601 gccgccagcc aggcgtcgac ctccgggctg tggcccacgg actcagccgg tctccgcggg
     3661 cgtggcatcg gtgatcgact ccccggagtg gatcccagtt cggcggtgga agggcgccgt
     3721 cgccgcgtcg ttcatctcga accgcgaggg cggcgcagcc gccgcgagtc gcgcgatctc
     3781 gtcctcggtc aggtcgtagc cgaagcgaat ggcggccacg ttttcgtcct ggtagcgccg
     3841 cggcagggag tcgcgaatgg ccccctcgtc atactcgatg ccggccagct tcagcagccg
     3901 gccgagcgcg atcatgttgc cgaacagctc tttgccgaag tgcgcggacc cgagcgaggc
     3961 gaacggaatc tgctcatcgc cgggcaggcc caggcccagg tcggtcacga ccttgcggcc
     4021 gctgatccgg agatgcccgc ggtccgcgag gcgcagcagg atgtcggcct cttcgaccac
     4081 cgggttgtcg atcgggacgt cgtcgaagat gacgaaggcg tcggacatgg agccgcggac
     4141 cgaggagtcg tagtcgagca gcacggtgac ctgcttgccc atgcgggcca ggaggctgcc
     4201 caggatgccc gcgatgagcc gcactccctg gccgcccacc ccgtcgagcg cgatgcgggt
     4261 tgccatccgg ccgcgcgccg ccctactcga gctcggccgg gtcgatcccg gccgccttga
     4321 gggcctcagg cagggtgagc tcgcccttgg caacctggag cttgaccgcc ggatcgaggc
     4381 gctgcatgag ggccaggcgc ttctgggcct ccgcccgctt gcgggccttc tcgtcatcgg
     4441 agacgaccgg acgctccgcc cgaggcgccg gagtcgcgcc ggcgccattg atcggcgccg
     4501 ggccatccgt ctgcgcgggc tcgtcggcaa gggcgccatt gctgtgctcg gcgtgcccct
     4561 cggggtagag gatccccagc tcgtgggggc cgagccgctc ggcccccttc ggggcgcgct
     4621 tgtacgtctt ccggtagaag gtgagcatct cgttcgcgtt ggcgaagccg atccgccggc
     4681 cgttgttctc gatgcactgc gacgtgatat cgacgaacga aaaaccgtcg tgctggatcg
     4741 cctcgcggat cgcggtccgc atctggggct ggtgatagac cgtcgtccgc gcgtagagcg
     4801 catgcggaga gctgcggacg atgccctgca ggttgatcgg ggtgttcggg tttccgccgg
     4861 gcgaggagag cgtcttcgtt ccattcgggg tggtcggccc ggtctggccg ccggtcagcc
     4921 cgtagatctc gttgttgttg cacaggatgg tgacccgagc gttccggcgg atggcgtgga
     4981 tgaggtgatt gccgccgatg gaggcggtat ccccgtcgcc cgccaccacg atcacggtga
     5041 gctcgggacg gacggtcttg atcgcctcgg ccacgggcag ggtccggcca tggagggtgt
     5101 agaccgcgtc gtatttcatg tacgagccca tccggccggt gcagccgatg ccggtcacga
     5161 tcacggtgtt cgactggtcc aggcccagct cgtcgaggac catcgccagc tcgatcatga
     5221 tcgtgccgat cccgcagcca gggcaccaga tggtcgggaa gaggtccatc ttgagcttgg
     5281 acttgaagcc catctcggcg aaggcggcct caggcatcgg tctcgctcat gctcagccga
     5341 tccccagtgg cgcgcggtcg gcgcgctggt tgggagccgg tgggaccggc ggctcgtcgg
     5401 ggccgtcgcc cagcacccgg gccagcccgg cgttgaccgc ctcgaggctg atccgtccac
     5461 cgaggagcgg cacgcgcttg acctcgcgcc gaagcgccag ctcagccatg tccgcgtact
     5521 gcccgtcgtt ggcttccacg gccacgatcg tctcgtagcc ctcggccacc gcgcggagct
     5581 cctcggtcag catggggtgg atccggatcg gccggaacag cgcgtactcc tcggccagcg
     5641 gcgcggccac ccgcgaaacg atcccgtagc cgatgaccag cgtcttggca tcggtgttcc
     5701 agccgtgctg gtacagcgcg tagcggtccg caacttcccg gtgccgatgc ttggcggcgt
     5761 agtactggcg caggtactcg tccgcgtcgg cggtggccgg ctctccggca ttcgggccgt
     5821 gctcgtacat caggaccccg gcgaagaagc gcgggtcggc cccgccgctg gccaacggct
     5881 ccaggtcgcg ggggatgatc gacaccccgg cctcgagctc ctccaggtcg acgacctcgt
     5941 tcaggtgggc gacaaaggtg tcggacagga ggaccaccgg cgagcgcgac tcctcggcgg
     6001 cattgaaggc cgccaccgcc aggttgaatg cttccggcac cgagttcgga tagaaggcga
     6061 tcggcgtgta gtcgccgctg ctgccgtagc gaacctgcag gatgtcgccc tgtcccggca
     6121 tcgtcggcat cccggtggcc ggtcccacgc gctggacatc gacgaacacg ctgggcacgc
     6181 ccaccttctg tgcgtagccg atggcctcct gcatgaggct gaagccggga ccgctggtgg
     6241 cggtaaatga cttggcgccg gcaagcgagg cgccgatgac cgagttcgcg ctcgcgatct
     6301 cgtcctcggc ctgcagaaag ccgaactccg ggtgctccgc cgcccagccg gacgcgatgt
     6361 tcaggatctc ggtcgaggga ctgatcgggt agccggcgta gtacgaagcc ccggcggcaa
     6421 tggccccccg gaacaccgcc tcgtttccct ggaccaggcg gcgcatcggt ctagccctcg
     6481 tctccaggcc cgtcggccct gactgcgttc cccgttccgg cgtctcccgc tccggcgtca
     6541 cccttggcgg cataggcggc gatcgcagcc gggatcatct cgatcgccag atccgggcag
     6601 tagcgctcgc agaggccgca caggatgcag ttgtcttctt cgaggatggc gtcgtcgcga
     6661 aggacgagcg ttcccttggg acagatgtcg acgcagatgt cgcagcgttt gcagaagacc
     6721 gggttccagc tgatgttctg gtaatcgatc cagcgcaccc cgtcctggtg gccggtcgag
     6781 gtgccggtcc cgaaatggag agcgtttgcc agtcgatccg cgctcatagg gggaccgatg
     6841 atagtcgcgt ggccccacga tcgggcagcg atgagccccg ccagcggccg gggcctgcac
     6901 ccgagcgccg gctcaggcgg gattcggctc cggttcgacc gccggctcgg gctctgacgc
     6961 tgttcgcggc cgaccggtga ccatcgcgac caccgcgccc atgatgatcg cggccgccag
     7021 ccaggtccgc ggcgtcatcg gctcactcag gacgatgacg cccagcgcga cggccacgat
     7081 cgggttcacg taggccgtgg tcgagatcac gctgatcgac acatggctca ggagccagaa
     7141 gtacgccgtg aacgcgacca gcgagccgaa caggatcagg tacgccagcg caatcagcga
     7201 ctcggtcgag aacgcgtccg ggttcacgtc cgccagctcg ccgcggagca gaccgatcag
     7261 cagcatcacc gcaccaccgg ccagcatctg aagcccggcg ccctggaacg gggaggtcgg
     7321 catcgggatc cggcgcgagt acaccgaccc gattgaccac gagatcgcgg cgcccgcgac
     7381 gagggcgagc ccgagtggat caattggcgg gctgccctcg agcgggacga cgaggatcgc
     7441 caccccgatc aggccggcca cgaggccggc gacggcgagg cggctcgggc gccggccggc
     7501 gacgaccgcc tcgaagatcg ccatccagat gggcgtggtg gcaatgatga gcgccgcgat
     7561 cccggtcgcg atcagctgct cgcccagcac gaccccgccg ttcccgccga ccaggagcag
     7621 gctcccgatg aggagagccg agcgccaatg ctggcgggtg ggccgctcga ggcgcccccc
     7681 acgaagccgg ctccacacga tcagcgcggc gagcaggatg accccggcgg tcgtatatcg
     7741 caccccggcc gccacaagtg gcggcagcgt ttcgaccatg accgcgatgc ccaggtaggt
     7801 cgatccccag accacataga cgatgccgag ggcggccgcg accttccagc cggcggcacc
     7861 ggatgcgttc gggaccatcg gcgcattgtg cccggcctcg gtagactgcg ccgagcatgc
     7921 gcattctgat cggcctgctc ggggcggtcg ccgtcgcgct ccttgtcctc ctcgtgcttc
     7981 cgctgttcgg cgtgtccatc ggctttgggc cgttcgccag cgtcgagccg tcggcgtcgg
     8041 ccagtgcgag cgcgagcgca tcggccagct cctccgggtc ggaggcggcg
//
LOCUS       DASYGU010000101         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_890670, whole genome shotgun sequence.
ACCESSION   DASYGU010000101 DASYGU010000000
VERSION     DASYGU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890670"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VGO32_02715"
     CDS             <1..667
                     /locus_tag="VGO32_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV7199696.1"
                     /translation="VDVHLTSDGELAVIHDATLDRTTDRTTTVATATMAAIREADAGW
                     AFPGEASEYPFRGTGMTVPTLPQVLAWLPEGLGLAVEIKAAPAADAVVAALAESAVRE
                     AGQVTVMSFLESAISRVRELAPKIPTGLLLVPGDSFERGLKWVVEHGHAAVLPFDPDL
                     GEDPAPNVALATSWGTRVGCYVVNEPERMQQLAAAGLWGFVTDVPDVARAALGPRPAA
                     GAE"
     gene            complement(669..1070)
                     /locus_tag="VGO32_02720"
     CDS             complement(669..1070)
                     /locus_tag="VGO32_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199697.1"
                     /translation="MHVFIIAPANQPGTLATALEAIAKRGVNVISGGGATWGNAGYIA
                     LQTNDDDGTRAALQESGTTFREVQAAAAWLDDQPGTFADAARRLGNAGVNIEAVFPIG
                     MKDGKVGILFGVDNADAAQAALGALTGAAAG"
     gene            1205..2089
                     /locus_tag="VGO32_02725"
     CDS             1205..2089
                     /locus_tag="VGO32_02725"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEV7199698.1"
                     /translation="MTYSIVARDPATGRFGIAVQSHYLGVGPVVPWLEAGVGAIATQA
                     QVDISFGPIGLELLRNGRTAEQTVAALLASDPTPQVRQLGVVDGEGHAFAHTGTDTIP
                     AAGHLIGEGFTVQGNLLERDTCWPAMKTAYESATAEGLPFSERLLRAMEAAEGEGGDV
                     RGRQSSAIMIVEGTLQPVPWKGRVLDARVDDHPDPVPELRRLVDLYEAYDLLDPEKGG
                     VSEAEGYAEARRRAPEAYELVFWMGIEHAKRGEIDDARRELAIAFAADARWRTTLRHL
                     ADAGREGMNPEMAAKLTE"
     gene            complement(2094..>2921)
                     /locus_tag="VGO32_02730"
     CDS             complement(2094..>2921)
                     /locus_tag="VGO32_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7199699.1"
                     /translation="DVENYPGFPDGILGPELMDLFRRQAERFGAHILEVDVTKVDFSE
                     RPFKLWVGDDAYLADAVIVATGASALWLGLDSEEQFRGRGVSACATCDGFFFRDRDVA
                     VVGGGDTALEEALFLTKFANKVTMLVRRDEFRGSKIMQDRAAAHPKIEIRWNREVAEV
                     LGDTTVQQLRLRDTGSGTEEVLDAQGMFVAIGYKPNTDLFVGQLDLDARGYCEVAGRG
                     PGETSSNIEGVFVAGDVHDHRYRQAVTAAGDGCKAAIDAERWLESIGEHIATTATNW"
BASE COUNT          432 a         1019 c         1036 g          434 t
ORIGIN      
        1 ggtcgacgtc cacctcacct ccgacgggga gctggcggtc atccacgacg ccaccctgga
       61 ccggacgacc gatcgaacga cgacggtcgc gaccgcgacg atggccgcca tccgcgaggc
      121 cgatgcgggc tgggcctttc cgggcgaggc gagcgagtac ccgttccgcg ggaccgggat
      181 gaccgtgccc accctgcccc aggtgctcgc ctggctgccc gaggggcttg gcctggcggt
      241 ggagatcaag gccgcgccgg cggcggatgc ggtggtcgcc gctctggccg aaagcgcggt
      301 ccgcgaggcc ggccaggtga ccgtcatgag cttcctggag tccgccatca gccgggttcg
      361 ggagctggcg cccaagatcc caaccgggct gctgctggtg ccgggcgaca gcttcgagcg
      421 cggactgaag tgggtcgtgg agcacggcca cgccgcggtg ctgccgttcg atccggatct
      481 cggcgaggac ccggccccga acgtggcgct ggccacgtca tggggcacgc gggtggggtg
      541 ctacgtggtg aacgagcccg agcgcatgca acagctggcc gcggccggcc tgtggggatt
      601 cgtcaccgac gtgccggacg tggcgagagc cgccctgggc cctcgccccg ccgcgggcgc
      661 ggagtaggtc agccggcggc cgcgccggtc agcgcaccga gagcggcctg tgccgcatcg
      721 gcgttgtcga ccccgaacag gatcccgacc ttgccgtcct tcatcccgat cgggaagacg
      781 gcctcgatgt tgacccccgc gttgccgagg cgtcgggcgg cgtcggcgaa cgtgcccggc
      841 tggtcgtcca gccaggcggc cgcggcctgc acctcgcgga aggtggtgcc gctctcctgg
      901 agcgccgccc gcgtcccatc gtcgtcattg gtctgaagag cgatgtagcc ggcgttgccc
      961 caggtggcac caccaccgga gatgacgttg acgccgcgct tggcgatggc ctccagggcg
     1021 gtggccaggg tgcccggctg atttgcggga gcgatgatga acacgtgcat ggaatctcct
     1081 tcctcgcgca aggccttggt ggatggctga gcgtacaccc ggccctgttg ccgggttgat
     1141 caagcgcact cgaaatgacc gataacccgg cgccaccaga tccctcggcg gctaccctcc
     1201 gcccatgacc tacagcatcg tggcccgcga cccggccacc gggcgatttg gcatcgccgt
     1261 ccaatcccac tacctgggag tcggtccggt cgtgccctgg ctggaggccg gcgtcggggc
     1321 cattgccacc caggcccagg tcgacatctc gttcgggccg atcggcctgg agctgctgcg
     1381 caatgggcgg accgccgagc agacggtcgc cgccctgctg gccagcgatc cgacaccgca
     1441 ggtacggcag ctgggcgtgg tcgatggcga gggccacgcc tttgcgcaca ccgggaccga
     1501 caccattccg gccgcgggcc atctcatcgg tgaaggcttc accgtccagg gcaacctcct
     1561 cgagcgcgac acgtgctggc cggccatgaa gaccgcctac gagtccgcca cggccgaggg
     1621 actgccgttc agcgagcggc tcctgcgggc gatggaggcg gcggaaggcg aaggtggcga
     1681 cgtccgcggg cggcagagca gcgcgatcat gatcgtcgag ggcacgctcc agcccgtgcc
     1741 atggaagggt cgtgtcctcg atgcacgggt cgacgaccac cccgacccgg ttcccgagct
     1801 gcgccggctg gtggacctgt acgaggccta cgacctgctc gaccccgaga agggcggggt
     1861 cagcgaggcc gagggctatg ccgaggcccg ccgccgggcg ccggaggcgt acgagctcgt
     1921 gttctggatg ggcatcgagc acgccaagcg cggggagatc gacgacgcgc gccgcgagct
     1981 ggccatcgcc tttgcggcgg atgcgcgctg gcggacgacc ctccgccatc ttgccgacgc
     2041 tggacgcgaa gggatgaacc ccgagatggc ggcgaagctg accgagtagt ccgctaccag
     2101 ttggtggcgg tggtcgcgat gtgctcgccg atggattcca gccagcgctc agcgtcgatc
     2161 gcggccttgc agccgtcgcc tgcggcagtc accgcctggc ggtagcgatg gtcgtggacg
     2221 tcgccggcca cgaacacgcc ctcgatgttg ctgctcgtct cacccggacc gcggccggcc
     2281 acctcgcagt agccccgggc gtcgaggtcc agctggccca cgaacaggtc ggtgttcggc
     2341 ttgtagccga tggcgacgaa catgccctgg gcgtcgagca cctcctccgt gccgcttccc
     2401 gtgtcgcgca gccgcagctg ctggaccgtc gtgtcaccga gcacctcggc cacctcgcgg
     2461 ttccacctga tctcgatctt ggggtgggcc gcggctcggt cctgcatgat cttcgagccg
     2521 cggaactcgt cgcgccgcac gagcatggtc accttgttcg cgaacttggt caggaacagc
     2581 gcctcctcga gcgcggtgtc cccaccgccg acgacggcca cgtcccggtc gcggaagaag
     2641 aacccgtcac acgtcgcaca ggcagacacg ccgcgtccgc ggaactgctc ctcggaatcg
     2701 aggcccagcc acagggcgct ggcgccggtg gccacgatca ccgcatcggc gagataggca
     2761 tcatccccca cccacagctt gaagggtcgc tcgctgaagt cgaccttcgt gacgtcgacc
     2821 tcgaggatgt gggccccgaa tcgctccgcc tggcggcgga agagatccat gagctcgggg
     2881 ccgaggatgc cgtccgggaa tccggggtag ttctcgacgt c
//
LOCUS       DASYGU010000102         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_325594, whole genome shotgun sequence.
ACCESSION   DASYGU010000102 DASYGU010000000
VERSION     DASYGU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325594"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..897)
                     /locus_tag="VGO32_02735"
     CDS             complement(13..897)
                     /locus_tag="VGO32_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199700.1"
                     /translation="MTAAATEAPPRISRRRRVIFFALFFVGLAVLWEGLKVLGGDPYR
                     VDGEVVWLPPMDIGMASDVNMPHVWVIFQRLWEPLTGGRDETLFGYLVDQTLYTLRAA
                     FIGFTIGGLLGIGLAVAFVHSGLVERAFVPYVVASQTIPIIALAPLIVYLVGQNVTSV
                     VIVATYLSFFPVTIAMLRGLRSPDPRALELMRSYAASKRAVLWKVRFPASLPYLFTAM
                     KITATASIVGAIVGEGSGSLQAGLGRALVFFSQQYTVAPEKLWGAILFCSFVGVAAFG
                     LVRLVEMRVVRVRPGMEP"
     gene            complement(948..2279)
                     /locus_tag="VGO32_02740"
     CDS             complement(948..2279)
                     /locus_tag="VGO32_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7199701.1"
                     /translation="MTVTPTRRLTAEEVVALNRKYTLFSWSAQAKINPIAIDHGEGIY
                     FWDPDGKRYIDFNSQLMSVNIGHGDRRVADAIAEQAHQLAFAAPQFATEVRGRLGELL
                     ATLTPGTLDKFFFTLGGAEANENALRLARMVTGRQKVIAHHRSYHGATAGSISVTGDP
                     RRWANEPGVPGVIRVLDPWRWGQTEPEPVDRQLEYLEEVIQYEGPHTIAAFILETVTG
                     TNGILIPPDGYLQGIRAICDRHGILMITDEVMAGFGRTGRWFAVDHWDVVPDLLTMAK
                     GLTSSYLPLGAVAMKPELAAEFDERVYYGGLTYSAHPVSCAAAIAAVGVLRDDDMIGN
                     AARLGPVMHDLLADIEARHPSVGTTRNIGLFGVIELVRDPRTREPMTAFNASSPEMAA
                     LDARLRADGLYTMQHWHQVFTNPPLCITEEQLRESFGIIDRALDETDAAVA"
     gene            complement(2353..3756)
                     /locus_tag="VGO32_02745"
     CDS             complement(2353..3756)
                     /locus_tag="VGO32_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV7199702.1"
                     /translation="MDDRERALQRAAELSLEFLAGLAERHVGARTDGNAIAAALAGPL
                     PEDGEDPVAVIEDMARRLDPGLVATAGPRYFGFVIGGAIPAAIGADWLTAAWDQNANL
                     HALSPAAAAAEQVTGGWMLDLLGLPPSASVGLPTGAGLANAVGLAAGRHAVLSREGWD
                     VEARGLYGAPEITVVIGEEAHATVLTSLQYLGLGRERVVKVPADDQGQMRADALAELV
                     AELTGPLIVVAQAGNVNTGGFDPMPAIADAVSAHPNTWIHVDGAFGLWAAVSPRLHHL
                     VSGVDRADSWATDAHKWLNLGYDCGFVAVRDSEAHRAAMSTFASYLMQNQTQRESWEY
                     VMDSSRRARGFTLYAALRSLGRSGVRALVERCCALASRMADSLASAEGVEVLNDVVLN
                     QVLVRFSPTDGPADPEAADAFTREVIRRVQDEGVAWMGGTTWHGLAAMRISVSNWSTT
                     EADADRTVDAILRAARS"
     gene            complement(3766..4359)
                     /locus_tag="VGO32_02750"
     CDS             complement(3766..4359)
                     /locus_tag="VGO32_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7199703.1"
                     /translation="MPWIPPAKIAFIGSHSVRKSNAVHAFASAVGRAGRSVEVSREVI
                     RFNPLGRNEGASAEAQLWVLMTQVQQELELGGRSDILVTDRAVVDNYAYFLRVTHGAD
                     PFGVEPLVRRWSQTYDLFVRLLPDVALMPDGVRSTNDAFRDEIEAILDRIVPTYVAAD
                     RCIRLPASEVTEGYDWNAVAEQLARIVGQRLRDPVPA"
BASE COUNT          711 a         1517 c         1539 g          643 t
ORIGIN      
        1 tgttcgcgac catcagggtt ccattcccgg ccgcacgcgg acgacccgca tctccaccag
       61 gcgcacgagc ccgaatgcgg cgacgccgac aaatgagcag aacaggatcg cgccccacag
      121 cttttcgggg gccacggtgt actgctgcga gaagaagacg agcgcgcgcc ccagcccggc
      181 ctgcaggctg ccggagcctt cacccacgat cgctcccacg atgctggccg tggccgtgat
      241 cttcatcgcg gtgaacaggt agggaagcga cgccgggaac cgcaccttcc acaggaccgc
      301 ccgcttggag gcggcatatg agcgcatgag ctccagggcg cgtggatcgg gggatcggag
      361 gccgcgcagc atggcgatgg tgaccgggaa gaaactcagg taggtggcca cgatcaccac
      421 gctggtcacg ttctggccga ccaggtacac gatcaacggc gcgagggcga tgatcgggat
      481 cgtctggctg gcgaccacgt acggcacgaa ggcccgctcg acgagaccgg agtggacgaa
      541 ggccacggcc agcccgatcc cgagcagacc gccgatcgtg aacccaatga atgcggcgcg
      601 cagggtgtag agggtctggt cgaccaggta gccgaacagg gtctcgtccc gaccgccggt
      661 caggggctcc cacaatcgct ggaagatgac ccagacgtgg ggcatgttca cgtcgctggc
      721 catcccgatg tccatcggcg gcagccagac cacctcgcca tcgacccgat acggatcgcc
      781 gccgagcacc ttcagcccct cccacaggac ggccagcccg acgaagaaca gggcgaagaa
      841 gatgacccgc cgacgccggc tgatgcgcgg cggcgcctcg gtggccgcgg cggtcatcgg
      901 tctttcataa ggcggtcaac cggccgccgg ccgggctggg cggtcgctca ggcgaccgcg
      961 gcatcggtct cgtccagcgc ccggtcgatg atcccgaacg attcgcgcag ctgctcctcg
     1021 gtgatgcaca ggggcgggtt ggtgaagacc tggtgccagt gctgcatggt gtacagcccg
     1081 tcggcccgca gcctggcgtc gagggcggcc atctccgggg agctggcgtt gaacgcggtc
     1141 atgggctccc tggtccgggg gtcgcggacg agctcgatga ccccgaacag gccgatgttg
     1201 cgggtcgtgc ccacgctcgg gtgacgcgcc tcgatatcgg ccagcaggtc gtgcatgacc
     1261 ggaccgagcc gagccgcgtt gccgatcatg tcgtcgtcgc gcaggacgcc gacggcggcg
     1321 atcgccgccg cgcagctgac cgggtgggcg gagtaggtca ggccgccgta gtagacgcgc
     1381 tcgtcgaact cggccgcgag ctcgggtttc atggccaccg cgccgagggg caggtacgag
     1441 ctggtcaggc ccttggccat ggtcagcagg tcggggacga cgtcccagtg atcgaccgcg
     1501 aaccagcgcc cggtgcgacc gaagccggcc atcacctcgt cggtgatcat caggatcccg
     1561 tgccggtcgc agatggcgcg gatgccctgg aggtagccat cgggcgggat gaggatgccg
     1621 ttcgtccccg tcaccgtctc gaggatgaac gccgcgatcg tgtgcgggcc ttcgtactgg
     1681 atgacctcct cgaggtactc gagctggcga tccaccggct cgggctccgt ctggccccag
     1741 cgccacgggt cgaggacccg gatgaccccg ggcacgcccg gctcgttggc ccaacggcgc
     1801 gggtcaccgg tgacactgat gctgcccgcg gtcgcgccgt ggtacgaccg atggtgggcg
     1861 atgaccttct ggcggccggt caccatccgc gccaggcgga gcgcattctc gttggcttcc
     1921 gcgccgccca gggtgaagaa gaacttgtcg agcgtgcccg gggtcagggt cgccagcagc
     1981 tcgcccagcc ggccgcggac ctcggtcgcg aactgggggg cggcgaaggc cagctggtgg
     2041 gcctgctcgg cgatggcgtc ggccacccgg cggtcaccgt ggccgatgtt gacgctcatg
     2101 agctggctgt tgaagtcgat gtaccgcttg ccatcgggat cccagaagta gatcccctcc
     2161 ccgtggtcga tcgcgatggg gttgatcttg gcctgcgccg accagctgaa gagcgtgtac
     2221 ttgcggttga gggcgacgac ttcctcggca gtcagccgac gggtgggagt gacggtcatg
     2281 cgtacctcgg gcaacggggc ggggcgtcgc gattacacca caacccgcat gccgcggaca
     2341 ccccgccccg ggctagctcc gggcagcgcg gaggatggcg tccacggtgc ggtcggcatc
     2401 ggcttccgtc gtcgaccagt tgctgacgct gatgcgcatc gccgccaggc catgccaggt
     2461 cgtcccgccc atccaggcaa ccccctcgtc ctggacgcgg cgaatgacct cccgcgtgaa
     2521 cgcgtccgcg gcctcgggat cggccggtcc gtcggtgggg gagaagcgaa cgaggacctg
     2581 gttcaggacc acgtcgttca ggacctcgac gccttctgcc gaggcgaggc tgtcagccat
     2641 gcggctcgcg agcgcgcagc accgctcgac gagcgcccgg acgccggacc gcccaaggga
     2701 gcgaagcgcg gcgtacagcg tgaaaccgcg cgcccggcgc gagctgtcca tgacgtactc
     2761 ccacgattcg cgctgggtct ggttctgcat gaggtacgag gcgaacgtcg acatggcggc
     2821 gcgatgcgcc tcgctgtccc gaaccgcgac gaatccgcag tcgtagccga ggttgagcca
     2881 cttgtgggcg tcggtcgccc aggaatccgc gcggtcgacg ccactcacga ggtgatgcag
     2941 ccgaggcgag accgccgccc acagtccgaa cgccccgtcg acgtgaatcc atgtgttggg
     3001 gtgcgcactc accgcgtcgg cgatggccgg catcggatcg aacccgccgg tgttcacgtt
     3061 gccggcctgg gcgacgacga tcaggggacc ggtgagttcc gcgaccagct cggcgagtgc
     3121 atcggcgcgc atctggcctt gatcatcggc gggaaccttg accactcgtt cccggcccag
     3181 gcccaggtac tggagggagg tcaggaccgt ggcgtgcgcc tcctcgccga tgaccaccgt
     3241 gatctccggt gccccgtaca ggccacgcgc ctcgacgtcc catccctcgc gcgagagcac
     3301 cgcatgacga ccggccgcca ggcccaccgc gttggcgaga cccgcccctg tcggcagacc
     3361 gacgctggcc gatggcggca gcccgaggag gtccagcatc cagcctcccg tcacctgctc
     3421 ggcggccgcc gcagccggcg acaacgcgtg gagattggcg ttctgatccc aggcagccgt
     3481 cagccagtcc gcgccgatgg cggcggggat cgcgccaccg atcacgaatc cgaaataccg
     3541 cgggccggcc gtcgccacga gacccgggtc gagccgccgc gccatgtcct cgatgacggc
     3601 cacggggtct tcgccatcct caggcagcgg ccccgcaagt gcggcggcaa tggcattccc
     3661 atcggtccga gcacccacgt gccgctcggc gaggccggcc aggaactcga ggctcagctc
     3721 cgcagcccgc tgaagggctc gctcgcggtc gtccatcggt tgagcctacg ccggaacggg
     3781 gtcccggagg cgctggccca cgatccgggc cagctgctcg gccaccgcat tccagtcgta
     3841 cccctcggtc acctcgctgg ccggcagccg gatgcaccgg tcggccgcca cgtaggtggg
     3901 cacgatccgg tccaggatgg cctcgatctc gtcgcggaac gcgtcgttcg tcgagcggac
     3961 gccgtcgggc atgagcgcca cgtccgggag gagtcggacg aacaggtcgt aggtctgtga
     4021 ccagcgccgg acgagcggct cgaccccgaa cggatcggcc ccgtgggtca cgcgcaggaa
     4081 gtacgcgtag ttgtccacga ccgcgcgatc ggtgacgaga atgtcggatc gcccgccgag
     4141 ctcgagctcc tgctggacct gggtcatgag cacccacagc tgcgcctcgg ccgacgcgcc
     4201 ctcgttgcgg cccagcggat tgaagcggat cacctcgcgg ctgacctcga cgcttcgccc
     4261 ggcgcggccc acggccgagg caaaggcatg gaccgcgttc gacttgcgga ccgagtgcga
     4321 gccgatgaat gcgatcttgg ccggcggaat ccagggcacg ggacagccta aacatttcct
     4381 tcatcgcaat cgatgtagcc tctgcacatg
//
LOCUS       DASYGU010000103         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1302815, whole genome shotgun sequence.
ACCESSION   DASYGU010000103 DASYGU010000000
VERSION     DASYGU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302815"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VGO32_02755"
     CDS             complement(<1..956)
                     /locus_tag="VGO32_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV7199704.1"
                     /translation="MIRPRFAWQLPPPIDPPPSLTGFSAPVATLLVRRGITTDEQAAR
                     FLNAGAHDLPPVSLMTDAEVALDRIERAIDAGESIAIWGDYDADGMTAVAVWVLALRR
                     LGVEPIRYVPSRLAEGYGLSVDGLGTLAAQGVRLVVTCDCGVGNAVEVAAAAELGLDV
                     VVTDHHVPLGPLPAAVAVVDPHRPDCTYPNQDLTGAGLAFRMATALLDRGGLTAPDLA
                     AVGALGTIADMAPMTGESRAIVRLGLAELAVTTHPGLRGLLARACEDPLHPTVRDVGF
                     GIVPRLNAAGRIADAELALDLLVATDAAEADRLLAELEAVHER"
     gene            complement(1025..2143)
                     /gene="secF"
                     /locus_tag="VGO32_02760"
     CDS             complement(1025..2143)
                     /gene="secF"
                     /locus_tag="VGO32_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEV7199705.1"
                     /translation="MYDIVGKRRWYYLFSAMITIPGVFFILIGGLKPSIDFTGGTVWQ
                     VRFLDDPTPAEVQAALVELGHEEATVTAVDNGFLEIRLAPIEFPIVGGIPTPTPSPLG
                     SPTASPSTSATATPSAAGSPAGSPGASPTATPTPSPTPTPTPSASPTPEPTPEPPPTI
                     QGTPIEELVTELNAQFGFSETRSLDTVGPIIGAELVRSSLIIIIIGEAFIFLYLWYRF
                     RSIRFGGAAIVALIHDVIVVVGAFAILGFFFGIEFDALFVTALLTVVGFSVHDTIVVF
                     DRIRENRLRHAGESFGAIVNHSILQTMGRSINTSLTVIATLAALLLLGPVTIQTFTLA
                     LLIGIISGTYSSIFNASQILVTWDEWAARRKARRTATR"
BASE COUNT          347 a          712 c          841 g          306 t
ORIGIN      
        1 cgctcgtgca ccgcctccag ctcggcgagg agccgatccg cctcggccgc gtcggtggcg
       61 accaggaggt ccagggcgag ctcggcatcc gcgatccgtc ctgcggcgtt caggcggggc
      121 acgatcccga atcccacgtc ccggacggtc ggatgcagcg ggtcctcgca cgcccgggcg
      181 agcagtccgc gcaagccggg gtgggtggtc accgccagct cggccaggcc gaggcgcacg
      241 atcgcgcggc tctcgccggt catcggcgcc atgtcggcga tcgttccaag cgcgccgacg
      301 gcggcgaggt cgggtgccgt caggccgccc cgatcgagga gcgcggtcgc catccggaag
      361 gcgagcccgg caccggtgag gtcctggttg gggtacgtgc agtccggccg gtgcggatcg
      421 acgacagcca ccgcggccgg cagcgggccc agcgggacgt ggtggtcggt gaccacgacg
      481 tcgaggccga gctcggcggc cgcggcgacc tcgaccgcgt tgcccacgcc gcagtcgcag
      541 gtcaccacca ggcggacgcc ctgcgcggcg agggtgccga ggccgtccac tgagaggccg
      601 tatccctcgg cgagtcggga ggggacgtat cggatgggct caactccgag ccggcggagg
      661 gcgaggaccc agacggccac cgcggtcatg ccatcggcgt cgtagtcgcc ccagatcgcg
      721 atcgactcgc cggcatcaat cgcccgctcg atgcggtcca gcgcgacctc ggcatcggtc
      781 atgagcgaca ccggcggcag gtcgtgagcg ccggcgttca ggaagcgggc ggcctgctcg
      841 tcggtcgtga tcccgcgtcg gacgagcagg gtggcgaccg gggcactgaa cccggtgagg
      901 ctcggtggcg ggtcgatcgg gggcgggagc tgccaggcga accggggccg aatcatgcag
      961 gcccatggta gaggcgggcg gtccgctccg gattaccgcc gcggcacggg cggtccgggc
     1021 gtggtcagcg ggtcgcggtg cggcgggcct ttcgccgggc cgcccattcg tcccaggtga
     1081 cgaggatctg gctcgcgttg aagatcgagg aatacgtccc gctgatgatg ccgatgagca
     1141 gggccagggt gaacgtctgg atcgtgaccg ggccgagcag gaggagggcg gcgagggtcg
     1201 cgatcacggt cagcgaggta ttgatcgagc ggcccatggt ctgcaggatc gagtggttca
     1261 cgatcgcgcc gaacgactcg ccggcatggc gcagccggtt ctcccgaatg cggtcgaaca
     1321 cgacgatcgt gtcgtggacc gagaagccca ccacggtcag cagggcggtc acgaacaggg
     1381 cgtcgaactc gatgccgaag aagaagccga ggatcgcgaa ggcgcccacc accacgatca
     1441 cgtcatggat cagggcgacg atggccgcgc cgccgaagcg gatgcttcgg aaccgatacc
     1501 acaggtacag gaagatgaac gcctcgccga tgatgatgat gatcagcgag ctccgcacca
     1561 gctcagcgcc gatgatcggc ccgacggtgt ccagcgaccg cgtctccgag aagccgaact
     1621 gcgcgttgag ctcggtcacc agctcctcga tcggcgtccc ctgaatggtg ggcggcggct
     1681 cgggcgtggg ctccggcgtc ggcgacgcgc tgggggtcgg ggttggcgtt ggtgacggcg
     1741 tcggcgtggc ggtcggtgag gcgcccggag acccggctgg gctgccggcc gcggaagggg
     1801 tcgcggtggc cgatgtcgat gggctcgcgg tcggcgatcc caacgggctc ggcgtgggag
     1861 tcgggatgcc gccgacgatc gggaactcga tgggcgccag tcggatctcc aggaatccgt
     1921 tgtccacggc cgtgaccgtc gcttcctcgt gcccgagctc gacgagcgcg gcctggacct
     1981 ccgcaggagt gggatcgtcc aggaatcgga cctgccagac cgtcccacca gtgaagtcga
     2041 tggacggctt gagaccgccg atcaggatga agaagacccc cgggatcgtg atcatggcgc
     2101 tgaagaggta gtaccagcgg cgcttgccga cgatgtcgta catcggtcag cgctccacgt
     2161 ggtagtactc gatccgccgg ccccactcgc gatgggtgac catccg
//
LOCUS       DASYGU010000104        10285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1611432, whole genome shotgun sequence.
ACCESSION   DASYGU010000104 DASYGU010000000
VERSION     DASYGU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611432"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="prfA"
                     /locus_tag="VGO32_02765"
     CDS             <1..738
                     /gene="prfA"
                     /locus_tag="VGO32_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV7199706.1"
                     /translation="AGTGGDEASLFAADLYRMYGLYAERRGWKVELLSTSEGGSGGLK
                     EVIAEVNGDAAYSRLKFESGVHRVQRVPTTESSGRIHTSTATVAVLPEAEEVEVEIDE
                     SDLRIETYRSSGPGGQSVNTTDSAVRITHVPTGVVVAIQDEKSQHKNRAKAMSVLRAR
                     LLEAQRARLAEERGEVRRAQVGTGERSEKIRTYNFPDDRVTDHRIGLTVHNLPGLLAG
                     DLDRVIEPLAEADQAARLAEAGADVAG"
     gene            725..1657
                     /gene="prmC"
                     /locus_tag="VGO32_02770"
     CDS             725..1657
                     /gene="prmC"
                     /locus_tag="VGO32_02770"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV7199707.1"
                     /translation="MSPAESPPSSGVTVGERTVGAALAHARDTLQGAGSPSARLDAEV
                     LVAWSMERERSWLLAHLDDQLPAEAATVLSSLVTRRAMGEPVAYIRGFKEWYGLRIAT
                     DDRALIPRPETELLVDAAVADIAERMVSDDEPIEVREVGTGSGAVSVALARRFRAALT
                     LGRLRLVASDVSADALELAADNLAAHGLAHLVTLAVADLLDPVASLPQADVVVANLPY
                     VRSAEVATGHGSLAWEPALALDGGADGLDLVRRLLSRFPDRLAGHGSALLEIGADQAE
                     PVVAEVAGLPGTWSAVTTRDLAGHERIIRLARDR"
     gene            1654..2277
                     /locus_tag="VGO32_02775"
     CDS             1654..2277
                     /locus_tag="VGO32_02775"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV7199708.1"
                     /translation="MTDRLPNTEEGIVQAAALISGGALVAFPTDTVHGVACRAGDAEA
                     LERLFVLKGRPPDRRIAWLVTDEAQATGMGAVFDDRARALTAALWPGGLTLVLPRAAD
                     SAPAESDATTLGVRAPDHPVTRALLAAAGPLPVTSANPHGFPECYTSDDVLIAFAGAA
                     DLAAVVDGESPGGVASSVLDLSGETPRLLREGAIGRDRLEALVGPID"
     gene            2283..2735
                     /gene="rpiB"
                     /locus_tag="VGO32_02780"
     CDS             2283..2735
                     /gene="rpiB"
                     /locus_tag="VGO32_02780"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEV7199709.1"
                     /translation="MRVAIGSDHAGFVLKTLLCRMLDEMGVEYRDFGTTNEEPVDYPD
                     FIAPVARAVARGEFDRGIVLGGSGTGEAIVANKVRGIRCVQAATPEVARLGREHNDAN
                     VLSLGARVTTADVAEACVREFLKGEFEGGRHVRRVAKIAALEAEESGR"
     gene            2732..4003
                     /locus_tag="VGO32_02785"
     CDS             2732..4003
                     /locus_tag="VGO32_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEV7199710.1"
                     /translation="MSLQLEPEERAMLAGDAGAGPALAMRLVVRAAEVLEADRLIPIT
                     GAHVDSCLYHGEATIDFVDRLVDGGATVRVPTTLNVGAVDLIHPELWRGDPEVGARGR
                     RLMEQYRALGCRPTFTCAPYQLADARPGLGEQVAWGESNAIVFANSVLGARTARYGDF
                     MDIAAAVTGRVPDAGLHRAEERRARVVLRLAEDIPLPLFDDDSLYPVIGLVVGRLVGG
                     RVAALVGLPPGIDEDRLKALGAAAASSGAVALLHVVGSTPEAPTLTDALQGGEQDDEQ
                     IVTLDALREARAAVGGEVGGSIVAASLGTPHASAAELVAIADLLAGRPVADGVELLVS
                     TSRDTLAGAEQSGVAARLRASGVELLVDTCSYISPILRSPSGPVMTDSGKWAYYAPGN
                     IGAAVVFGSRAECVASAVAGHVVRDPTWGTA"
     gene            4012..4401
                     /locus_tag="VGO32_02790"
     CDS             4012..4401
                     /locus_tag="VGO32_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEV7199711.1"
                     /translation="MVAPGSARAPALVLDEPLSLWGGLDPETGAICEPRHPQHGAVVT
                     GRILVMPAARGSSSSASVLAEAVRAGTAPAGIVLGEPDLILAIGAAVAEELYGVRVPI
                     AVASSATLAAITDGQAVDIGSGGVSAD"
     gene            4434..5696
                     /gene="glyA"
                     /locus_tag="VGO32_02795"
     CDS             4434..5696
                     /gene="glyA"
                     /locus_tag="VGO32_02795"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV7199712.1"
                     /translation="MDAWAALQAEDPEIWQVVQDEEHRARSNLELIASESYPSRAVLE
                     ATGSVLTAKYAEGYPRRRYYGGCEVVDRAEDLARDRAKALFGAEHANVQPHAGAQANM
                     AAFHALLEPGDTVLGLALDQGGHLTHGSPVNFSGQYYTFVPYHVDRTTHLIDLDEVRT
                     LAREHRPRLIVTGATAYPRHWDFAAFREIADEVGAKLMTDMSHFAGLVAGGVHPSPVP
                     HADVVTTTTHKTLRGPRGAMILCTEALAKDIDRAVFPAIQGGPLMHVVAAKAVAFHQA
                     MSPEFKADAARTVDNARALGSSLAERGASLVSGGTDTHLLLVDVTPFGVTGKEANAAL
                     GEVDITVNKNTIPYDPNPPMVASGIRVGTPCVTGRGMGAAEMAQIGEMIVSGIEARGD
                     AAAQAAVRERVVALAERFPVPGLAWTRA"
     gene            6022..6216
                     /locus_tag="VGO32_02800"
     CDS             6022..6216
                     /locus_tag="VGO32_02800"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEV7199713.1"
                     /translation="MNNWGIALDLGIRLGVAVMIGFFGGLLLDGVFGTRPLLTLVGTA
                     FGVAAAMYTIWDVARKAMRR"
     gene            6283..7050
                     /gene="atpB"
                     /locus_tag="VGO32_02805"
     CDS             6283..7050
                     /gene="atpB"
                     /locus_tag="VGO32_02805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV7199714.1"
                     /translation="MEFTNSMVGALLASAILLISTVFVRRRGTLVPGRFQSLIELPIE
                     WLAGVVKGTGGKRWAEFAGLVIAIFLFVLVANMIGLLPGVGVIGTVVETEHGEQELIP
                     LVRAAAADLNFTLGLALISFVAFVAWGIRANGLGGYLKELLIAEPAYMTPLLTPIHII
                     SELSRLISLSMRLFGNVFAGEVLLAVILSLVPLLVPIVFLGLELIFGFVQALVFALLT
                     MTYITLAIAEHRQHGTGDAHPGEAPDHSEASPAPSRA"
     gene            7098..7322
                     /gene="atpE"
                     /locus_tag="VGO32_02810"
     CDS             7098..7322
                     /gene="atpE"
                     /locus_tag="VGO32_02810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEV7199715.1"
                     /translation="MEFQLLAAGLAIGLGTIGPGIGIGIGFSKAMEAIGRNPEASGTL
                     FVPYILGLALTEAMAIYALVIGLILIFAVS"
     gene            7333..7830
                     /gene="atpF"
                     /locus_tag="VGO32_02815"
     CDS             7333..7830
                     /gene="atpF"
                     /locus_tag="VGO32_02815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV7199716.1"
                     /translation="MAFFEAFGVDVWKLAFQIVNFLVLLYLLNRFLFKPALRRIDERQ
                     GKLSQGLEDAAAAARDRELARAEREAALAEARQESDALVQRAAKTAEATSAEILAEAR
                     ASAEQLLTRARDEIAAEKEKALTEIRGEVASLALDAAGKLVGSEMDSPTQRRLVEQFL
                     SENRN"
     gene            7833..8369
                     /gene="atpH"
                     /locus_tag="VGO32_02820"
     CDS             7833..8369
                     /gene="atpH"
                     /locus_tag="VGO32_02820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEV7199717.1"
                     /translation="MARRETAARRYAEAAFQVAVADGALNRWQADFAAISEALDVPAL
                     KAVMVHPAVPFADKERVLRRSVSEVATGPMNLVLLMIRRGRPGAIPAMRSHFEELVRR
                     HRGIARAEVRTALPIDDQDRAALERTLEHLAGRQVEFTELVDPSLIGGISVRIGDRLY
                     DASVRSRLERLRARLTAV"
     gene            8380..10110
                     /gene="atpA"
                     /locus_tag="VGO32_02825"
     CDS             8380..10110
                     /gene="atpA"
                     /locus_tag="VGO32_02825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV7199718.1"
                     /translation="MAIRSDEITSIIRSEIAGFSEGMDVAGVGTIVEVGDGIAQVYGL
                     SGALASELLEFPGGVMGMAFNLEEETVGALILGDYTELKEGDQVKTTGRVVEIPVGDA
                     LIGRVVNPLGVPIDGKGALETDRFRPVERIAPGVIVRQPVDTPVQTGIKAIDSMIPIG
                     RGQRELILGDRQTGKTAIAVDTIINQRGQDLICVYVAIGQKMSTVAQLVAQLEENGAM
                     EHTIVVVAGASDPAPIQFLAPYAGAAIGEEFMDAGRDALCIYDDLSKHAWAYRQVSLL
                     MRRPPGREAYPGDIFYAHSRLLERAARMSEEQGGGSLTALPIIETQAGDLSAYIPTNV
                     ISITDGQIHMQTDLFNQGQRPAVNAGLSVSRVGGNAQIKAMRQVAGRLRLDLAQYREL
                     AAFAQFASDLDKATRDQLTRGEKLTEILKQPQFAPLSVERQVAIIWAGTNGYLDDVPT
                     PKIKEFETGLYRFLDQNHPDILPGIASEKSISDAATDVLKEALTSYRSDAGYGSTDGV
                     ESAESAAAEAPASGDGHDVDADAANADAPAAETPAAEAAAADAPVSVDPWPEKADDDA
                     PATPKEDPTA"
     gene            10110..>10285
                     /locus_tag="VGO32_02830"
     CDS             10110..>10285
                     /locus_tag="VGO32_02830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV7199719.1"
                     /translation="MASLRDIRRRIGSIRNIAQITRAMEMVAASRMKRAQESILAARP
                     FAEELDDVLARVAAA"
BASE COUNT         1524 a         3563 c         3655 g         1543 t
ORIGIN      
        1 gccgggaccg gcggggacga agcctcgctg ttcgccgccg acctgtaccg gatgtacggc
       61 ctctacgccg aacgacgcgg ctggaaggtc gagctgctgt ccacctcgga gggcgggtcc
      121 ggcggtctca aagaggtcat cgccgaggtc aacggcgacg cggcgtattc gcggttgaag
      181 ttcgagagcg gcgtccaccg cgtccagcgc gtcccaacca ccgagtccag cggccggatc
      241 cacacctcca ccgccaccgt ggcggtcctc cccgaggcgg aagaggtgga ggtcgagatc
      301 gacgagtccg acctgcggat cgagacctat cggtcaagcg gaccgggcgg gcagtcggtg
      361 aacacgaccg attcggcggt gcggatcacc cacgtcccca ccggcgtggt ggtggccatc
      421 caggacgaga agagccagca caagaaccgg gcgaaggcca tgtccgtcct ccgcgcgcgg
      481 ctgctggagg cgcagcgggc gcgcctggcc gaagagcgtg gcgaggtccg tcgcgcccag
      541 gtgggcaccg gggagcggtc ggagaagatc cggacctaca actttcccga tgaccgggtg
      601 accgatcatc gaatcgggct cacggtccac aacctgcccg gcctgctggc gggcgatctg
      661 gaccgggtca tcgagccctt ggccgaggca gaccaggccg cccgactggc ggaggcggga
      721 gccgatgtcg ccggctgagt cgccgccctc gtccggggtg acggttggcg agcggacggt
      781 gggcgcggcc ctggcccacg cccgggacac gcttcaggga gccggttcgc cgtccgcacg
      841 gctcgatgcc gaggtgctgg tcgcatggtc gatggagcgc gagcgatcgt ggctgctcgc
      901 ccacctcgat gaccagctgc ctgccgaggc ggctaccgtc ctatcgtcgc tcgtcacccg
      961 ccgagcgatg ggcgagccgg tcgcgtacat ccgcggcttc aaggagtggt acgggctccg
     1021 gatcgccacc gacgatcgcg ccctgatccc gcggccggag accgagctgc tggtggacgc
     1081 cgccgtggcc gacatcgccg agcgcatggt cagcgacgac gagccgatcg aggtccgcga
     1141 agtcggtacc gggagcggag ccgtgtcggt ggccctcgct cgacggttcc gggcggcact
     1201 caccctcggc cggcttcggc tggtggcgag cgacgtgtcg gccgacgcgc tggagctggc
     1261 cgcggacaac ctcgccgccc acgggctggc ccacctcgtc actctggccg tcgccgatct
     1321 gctcgatccg gtggcgtcat tgccgcaggc ggacgtggtg gtcgccaacc tgccctacgt
     1381 gcgaagcgcc gaggtggcca ccggccacgg cagcctggcc tgggagcctg ccctggcgct
     1441 cgacggcggg gccgatggct tggacctcgt ccgacggctg ctttcccgtt tccccgaccg
     1501 gctggcgggg cacggcagcg cactgctcga gatcggcgcg gaccaggcgg agccggtcgt
     1561 ggccgaggtc gccggcctgc ccgggacctg gtccgcggtc acaacccgcg atcttgcggg
     1621 gcacgaacgg atcatccggc tggcacggga ccgatgaccg accggttgcc gaacaccgag
     1681 gaaggaatcg tgcaggcagc cgcgctgatc tcgggcggag ccctggtcgc cttcccgacc
     1741 gacacggtcc acggcgtggc ctgccgtgcc ggcgacgcgg aggccctgga acggttgttc
     1801 gtgctgaagg gccgcccgcc cgatcgccgg atcgcgtggc tcgtgacgga cgaggcgcag
     1861 gcgaccggga tgggagcagt cttcgacgac cgggctcgcg cgctgaccgc ggcgctgtgg
     1921 cccggcgggc tgaccctcgt gctgccccgc gccgccgatt ccgcgcccgc ggaatcggac
     1981 gcgaccacgc tgggcgtccg cgcccccgac catccggtca cccgcgctct gctggcggcg
     2041 gccgggccgt tgccggtcac cagcgccaac ccgcacgggt tcccggagtg ctacacgtcc
     2101 gatgacgttc tcatcgcatt cgccggcgcg gccgatctcg cggccgttgt cgacggcgaa
     2161 tcgcccggcg gcgtggcgtc ctcggtcctc gacctgtccg gcgagacgcc gcggctgctg
     2221 cgggaaggtg ccatcggtcg ggatcggctc gaggccctgg tggggccgat agactgaccg
     2281 ccgtgcgcgt cgccatcgga tcggaccacg ccgggttcgt cctgaagacc ctcctgtgcc
     2341 ggatgctgga cgagatggga gtcgagtacc gcgacttcgg gacgacgaac gaggagccgg
     2401 tggactaccc cgatttcatc gcgcccgtgg cccgcgcggt ggcccgtgga gagttcgacc
     2461 gcgggatcgt gctggggggg agcggtacgg gagaagcgat cgtggccaac aaggtccgag
     2521 gcatccggtg cgtccaggca gccacgccag aggtcgctcg gttgggccgg gagcacaacg
     2581 acgcgaacgt gctgtcgctc ggggcccgag tgaccaccgc cgacgtcgcc gaggcctgcg
     2641 tcagggagtt cctgaagggc gagttcgagg gcggacggca cgtccgccgg gtggcgaaga
     2701 tcgccgccct cgaggcggag gagtccggcc gctgagcctc cagctcgagc ccgaggagcg
     2761 ggcgatgctc gccggcgacg ccggggccgg gcccgcactc gccatgcgcc tggtcgtccg
     2821 ggccgccgag gtgctcgagg ctgaccggtt gatcccgatc accggcgcgc acgtggattc
     2881 gtgcctctac cacggggagg cgaccatcga cttcgtcgac cgcctggtcg atgggggcgc
     2941 gaccgtccgg gtcccgacca ccctcaacgt gggagcggtt gatctcatcc atcccgagct
     3001 ctggcgcggg gacccggaag tcggggcgcg tggacggcgg ctgatggagc agtaccgcgc
     3061 gctcggctgc cggccgacgt tcacctgtgc cccgtaccag ctcgccgacg cccgtccggg
     3121 gctaggtgag caggtcgcct ggggcgagtc gaacgcgatc gtgttcgcga actcggtgct
     3181 gggcgcgcgc acggcccgct acggcgactt catggacata gcggccgccg tcaccggccg
     3241 agtgccggac gccgggctcc atcgtgccga ggaacgccga gctcgggtgg tcctccgcct
     3301 cgcagaggac atccccttac cgctgttcga tgacgacagc ctctacccgg tcatcggtct
     3361 cgtggtcgga cgcctggttg gcggtcgggt ggccgccctg gtcggactgc cgccgggcat
     3421 cgacgaggac cggttgaagg cgctcggcgc ggccgccgcc tcgtccggcg ccgtcgcgct
     3481 gctccatgtc gttgggtcca cgcccgaggc gcccacgctt accgacgcgc tccagggtgg
     3541 tgagcaggac gacgagcaga tcgtgaccct ggacgccctg cgcgaggctc gagccgccgt
     3601 cggcggggag gtcgggggct cgatcgtggc cgcgagcctg gggacgccac acgcgtccgc
     3661 cgccgagctg gtggcgatcg ccgacctgct cgccggacgg ccggtggcgg acggcgtcga
     3721 gctcctggtg tccacgtcac gcgacacgct cgccggcgcg gagcagagcg gggtggcggc
     3781 acggctccgc gcctccggcg tcgagctgct ggtggacacc tgctcataca tctcgccaat
     3841 cctccgctcg ccatcgggac cggtcatgac cgattccggg aagtgggcgt actacgcgcc
     3901 gggcaacatc ggcgccgcgg tcgtgttcgg cagccgggcg gaatgcgtgg cctcggcggt
     3961 ggccgggcac gtcgttcgcg acccaacctg gggcacggcg tgaccggccg catggtggcg
     4021 cccggatcgg cccgcgcccc ggccctcgtg ctggacgaac ccctgtcgct atggggcggg
     4081 ctggaccccg agaccggcgc catctgcgag ccccggcatc cccagcacgg cgccgtcgtc
     4141 accggccgga ttctggtcat gcccgccgcc cgcgggtcat cctcgtccgc ctcggtgctg
     4201 gcggaagcgg ttcgggcggg gacggcgccg gccgggatcg tcctcggcga gccggatctg
     4261 atcctcgcca tcggcgcggc tgtggccgag gagctgtatg gggtccgggt cccgatcgcc
     4321 gtcgcgtcgt cagccaccct ggccgcgatc accgacgggc aggcggtcga catcggttcg
     4381 ggcggagtgt ccgccgacta gactcgggct ccaccagccg aggagatgga ccgatggatg
     4441 cctgggccgc gctgcaggcc gaagaccccg agatctggca ggtcgtccag gacgaggagc
     4501 atcgcgcccg gtcgaacctc gagctgatcg cgtccgagag ctacccgagc cgcgccgtgc
     4561 tcgaagcgac cggatcggtc ctcaccgcga agtacgccga gggctacccc cgacggcggt
     4621 attacggtgg ttgtgaggtg gtggaccgcg ccgaggacct cgcccgcgat cgggcgaagg
     4681 ccctgttcgg ggcggagcac gccaacgtcc agccgcacgc cggcgcgcag gccaacatgg
     4741 cggcctttca cgccctgctg gagccgggcg acacggtgtt gggcctcgcc ctcgaccagg
     4801 gcggccacct gacccatggc tcgccggtca acttcagcgg ccagtactac acgttcgtgc
     4861 cgtaccacgt cgatcggacg acccacctca tcgacctcga cgaggtccgg acgctggccc
     4921 gcgagcaccg gcctcgactg atcgtcaccg gcgccacggc ctacccgcgc cactgggact
     4981 ttgccgcctt ccgcgagatc gccgacgagg tcggggccaa gctgatgacc gacatgtcgc
     5041 acttcgccgg gctggtggcg ggcggtgtgc acccgagccc ggtgccccac gcggacgtcg
     5101 tcacgacgac cacccacaag acgctccgcg ggccgcgggg agccatgatc ctgtgcaccg
     5161 aggcactggc caaggacatc gaccgtgccg tgttcccggc catccagggc ggcccgctga
     5221 tgcacgtcgt ggcggccaag gcggtcgcct tccaccaggc catgagcccg gagttcaagg
     5281 ccgatgcggc ccggacggtc gacaacgcgc gggcccttgg cagttcgctg gccgaacgcg
     5341 gcgcctcgct cgtatcgggc ggaaccgata cccatctgct cctggtcgac gtcacaccgt
     5401 tcggggtcac cggcaaggag gctaatgcgg cgctcggcga ggtcgatatc acggtgaaca
     5461 agaacacgat cccgtacgac cccaacccgc cgatggtggc gagcggaatc cgggtcggca
     5521 caccctgcgt gaccgggcgc gggatgggtg ccgccgagat ggcccagatc ggggagatga
     5581 tcgtgtccgg gatcgaggcg cgcggcgacg cggcggccca ggccgcggtt cgcgaacggg
     5641 tggtggcgct cgccgagcga ttcccggttc ccggtctggc gtggacccga gcctgagcga
     5701 ggctggcggc ccggacccgg gagggcgggc gcgaaaggca ggcgctatac tcgccgcggc
     5761 ttccgatcgc ccggagtcga gctgaatgcc caaatcgagc cccgggccgg ccaggatcct
     5821 gggtgacgtc actctgatca tggtggtccc gatgctcggc ggcgtggttc tcggtctcgt
     5881 gctcgatgca caggctggaa cctcgccgat tttcgtgctc agcggtctgg cgatcggcag
     5941 cctgacttcg gccatcggga tctggctgta cattcggacg cgaagaccgc gtccaccgag
     6001 cccggaccct ggcctggagc catgaacaac tgggggatcg cactcgacct ggggatccgg
     6061 ctcggggtgg cggtgatgat cggcttcttc ggcggcttgc tgctggatgg cgtgttcgga
     6121 acgcggcccc tgctgaccct ggtggggacc gccttcgggg tggccgccgc gatgtacacg
     6181 atctgggacg tcgcgcggaa agcgatgcgg agatagggca gagagggtca ccagctggtt
     6241 cacgtcgaca tcgccgcgga aacgctgttt acgatcggcc ccttggagtt cacgaacagc
     6301 atggttggcg cgctgctggc cagcgccatc ctgctgattt cgaccgtctt cgtccgccgc
     6361 cgtggcacgc tggttcccgg ccgcttccag tcactgatcg agctgcccat cgaatggctg
     6421 gccggtgtcg tgaaggggac cggcggcaag cgatgggccg agtttgccgg gctggtgatc
     6481 gcgatcttcc tgttcgtcct ggtcgcgaac atgatcgggt tgctgccggg agtcggggtt
     6541 attgggaccg tggtcgagac ggagcacggc gagcaggagc tcatccccct tgtccgcgcc
     6601 gcggccgctg acctgaactt cactctcggc ctggccctga tctccttcgt cgcgttcgtc
     6661 gcgtggggca ttcgggccaa cggcctgggc ggctacctca aggagctgct catcgccgag
     6721 ccggcgtaca tgaccccgct cctgaccccg atccacatca tcagcgagct ctcacggctg
     6781 atcagcctgt ccatgcggct gttcggcaac gtgtttgccg gcgaggtgct gctggcggtg
     6841 atcctgtcgc tcgtcccgct cctcgttccc atcgtcttcc tgggcctcga gctcatcttc
     6901 gggttcgtcc aggccctcgt cttcgcgctg ctcaccatga cctacatcac cctcgccatt
     6961 gccgagcatc ggcagcacgg cacaggagat gcccacccgg gcgaggcgcc cgaccactcg
     7021 gaggccagcc cggcaccgag tcgggcctga tacgcaggct ccgcccgtgg cggagcgcat
     7081 aggaggaacc gaacaccgtg gaattccagc ttctcgccgc cggtcttgcc atcggcctgg
     7141 gcaccatcgg tcccggcatc ggcatcggga tcggcttcag caaggccatg gaggccattg
     7201 gccgcaaccc cgaggcatcg ggcacgctgt tcgtgccgta catcctcggc ctggcactga
     7261 ccgaggcgat ggccatctac gccctcgtca tcggcctcat cctgatcttc gcggtcagct
     7321 aggcctccga ccatggcgtt cttcgaggcc ttcggggtcg acgtctggaa gcttgccttc
     7381 cagatcgtca acttcctcgt cctgctctac ctgctgaatc ggttcctgtt caagccggcc
     7441 ctgcgccgga tcgacgaacg acagggcaag ctcagccagg ggctggagga tgccgcggcc
     7501 gcggcccgag accgggagct ggctcgtgcc gagcgcgagg ctgcgctggc cgaggctcgc
     7561 caggagtcgg acgcgctcgt ccagcgggcg gccaagaccg ccgaggcgac gagcgcggag
     7621 atcctggccg aggcacgcgc ctccgccgaa cagctcctga cccgtgcccg agatgaaatc
     7681 gcggccgaaa aggagaaagc gctgaccgag attcggggag aggtcgcgag cctcgccctg
     7741 gatgcggccg gcaagctggt cggatccgag atggactcgc ccacccagcg tcggctggtg
     7801 gagcagttcc tgtcggagaa ccggaactga gcatggcgcg acgcgaaacc gccgcccgta
     7861 gatacgcgga ggccgccttc caggtggccg tcgccgacgg tgcgctgaac cggtggcagg
     7921 ctgatttcgc ggccatctcc gaggcgctcg acgttccagc cctcaaggcg gtcatggtcc
     7981 acccggccgt cccattcgcc gacaaggagc gcgtcctccg ccggtctgtg tcggaggtcg
     8041 cgaccggacc gatgaacctg gtcctgctca tgatccggcg tgggcgaccg ggggccatcc
     8101 cggccatgcg gagccacttc gaggagctcg tccggcgcca tcgcggcatc gctcgggcgg
     8161 aggttcgcac cgccctcccg atcgacgacc aggaccgggc ggccctggag cggacgctcg
     8221 aacaccttgc cggacgccag gtcgaattca ccgaactggt cgatcccagc ctcattgggg
     8281 gcatctcggt gcgaatcggg gatcggctct atgacgcgag cgtacggagt cgcctcgagc
     8341 ggctccgggc gcgcctgact gcggtctgag gagtactgga tggccattcg atccgacgag
     8401 atcacgtcga tcattcgcag cgagattgcc ggcttcagcg agggtatgga tgtcgccggt
     8461 gtcggcacca tcgtggaggt cggggacggg atcgcgcagg tctatggcct gtccggcgcc
     8521 ctggcgtccg agctgctcga attcccgggc ggggtgatgg ggatggcctt caacctcgag
     8581 gaggaaacgg tcggcgcgct catcctcggc gactacaccg agctcaagga aggcgaccag
     8641 gtcaagacca ccggacgcgt ggtggagatt ccggtcggcg atgcgctcat cggccgggtg
     8701 gtcaacccgc tcggcgtccc gatcgacggc aagggcgcgc tcgagaccga tcgcttccgg
     8761 ccggtcgagc ggattgctcc cggcgtcatc gtccgccagc cggtggacac gccggtccag
     8821 accgggatca aggccatcga ctcgatgatc ccgattggcc gtggccagcg ggagctgatc
     8881 ctcggcgacc gccagaccgg caagacggca atcgcggtgg acaccatcat caaccagagg
     8941 gggcaggacc tcatctgcgt gtacgtcgcg atcggccaga agatgtcgac cgttgcccag
     9001 ctcgtggccc agctggaaga gaacggcgcg atggagcaca cgatcgtggt cgtggccggc
     9061 gcgtcggacc cggcgccgat ccagttcctc gccccgtatg cgggtgccgc gatcggcgag
     9121 gagttcatgg acgccgggcg agacgccctg tgcatctatg acgacctgtc gaagcatgcg
     9181 tgggcctatc gccaggtttc gctgctcatg cgccggccgc cgggccgcga ggcctacccg
     9241 ggtgacatct tctatgccca ctcccggctg ctcgagcgcg cggcgcggat gagcgaggag
     9301 cagggcggcg gttcactgac cgccctcccg atcatcgaga cccaggccgg cgacctgtcg
     9361 gcctacatcc cgaccaacgt gatcagcatc accgacggcc agatccacat gcagaccgac
     9421 ctgttcaacc aggggcagcg gcccgcggtg aacgccggac tgagcgtgag ccgagtgggt
     9481 ggcaacgccc agatcaaggc catgcgccag gtcgccggcc gtcttcgtct ggacctggcc
     9541 cagtaccggg agctggccgc gttcgcccag ttcgcctcgg acctcgacaa ggcgactcgc
     9601 gatcagctga cccgcggcga gaagctgacc gagatcctca agcagcccca gttcgcgccg
     9661 ctctcggtgg agcggcaggt ggcgatcatc tgggccggca ccaatggcta cctcgacgac
     9721 gtgcccacgc ccaagatcaa ggagttcgag accgggttgt accggttcct cgatcagaac
     9781 caccccgaca tcctgccggg catcgcctcc gagaagtcga tctccgatgc ggccacggac
     9841 gtccttaagg aggcattgac ctcctaccgc agcgacgcag gctacggatc gacggatggc
     9901 gtcgaatccg cggagtccgc cgccgccgag gcgcccgcga gtggcgacgg ccacgacgtc
     9961 gacgccgatg ccgcaaacgc ggatgcaccg gctgccgaga caccggccgc cgaggccgca
    10021 gctgccgacg ccccggtatc ggtcgacccg tggcccgaaa aggcggacga cgacgcgccg
    10081 gcgaccccga aggaggatcc gaccgcctga tggcctccct ccgcgacatc cggcgccgca
    10141 tcggctccat ccggaacatt gcccagatca cccgggcgat ggagatggtg gccgcctcgc
    10201 gcatgaagcg cgcgcaggag tcgatcctcg ccgcccgccc gttcgctgaa gagctggacg
    10261 acgtgctggc ccgtgtcgcc gcagc
//
LOCUS       DASYGU010000105         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_582962, whole genome shotgun sequence.
ACCESSION   DASYGU010000105 DASYGU010000000
VERSION     DASYGU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582962"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGO32_02835"
     CDS             complement(<1..267)
                     /locus_tag="VGO32_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV7199720.1"
                     /translation="MSDQREPQFETLAVHAGFEPDETTGAVVPAIEQAVTFQQDAVGQ
                     PRNGYEYSRTGNPTRQLLERAVAELEGGTHGIAYASGLAATQNLL"
     gene            complement(310..1803)
                     /locus_tag="VGO32_02840"
     CDS             complement(310..1803)
                     /locus_tag="VGO32_02840"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV7199721.1"
                     /translation="MPRVNHWIGGKRVAGTSGRHGPVYNPATGEVAREVDFASVEEVD
                     AAVKAAHEAFPAWRATSLSRRTEIMFRIRNLVDQHRADIAAHLTAEHGKVPSDALGEV
                     ARGLENLEFATGVPQLMKGSFSEQASTGIDVYQIRQPLGVAAGITPFNFPAMVPMWMF
                     ANAIACGNTFVLKPSEKDPSASVYMAELLAEAGVPAGVFNVVHGDKVAVDRILEHPHV
                     ASVSFVGSTPIARYIYENGTKAGKRVQALGGAKNHMIVLPDADIEMAADAAVSAAYGS
                     AGERCMAISVVVAVGDVAQPLVEAIQARLPKIKVGPGTDPEAEMGPLITREHRDKVAS
                     YLESGPAQGAELVVDGREHPLYQDGQGFFLGVSLLDKVRPDMDCYQNEIFGPVLEVVR
                     ADTYTDAVEIIHDNPYANGTAIFTRDGGAARQFQFDIGVGMVGINVPIPVPVAYYSFG
                     GWKSSLFGDLHMYGPEGIQFFTRAKVVTARWPDPGTSSVDLGFPRTR"
     gene            2004..2438
                     /locus_tag="VGO32_02845"
     CDS             2004..2438
                     /locus_tag="VGO32_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEV7199722.1"
                     /translation="MADQSLYDRLGGIFAISAVVDHFSDALIKNPIVGQQSANPALRQ
                     WHSDQLGRLPGLKFMRTLWVAEVSGGPFTYQGTEPGRTALGLEQAHREFKITPAEFDE
                     VAAELARTLDTVGVPEQEKGEVLAAFAAHKDEVNEGSLAAAS"
     gene            complement(2541..3238)
                     /locus_tag="VGO32_02850"
                     /pseudo
     CDS             complement(2541..3238)
                     /locus_tag="VGO32_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
BASE COUNT          490 a         1080 c         1136 g          532 t
ORIGIN      
        1 cagcaggttc tgggtggccg ccaggccgct ggcataggcg atcccgtgcg tgccgccctc
       61 gagctcggcg accgcgcgct cgagcagctg gcgggtcggg ttgccggtgc gcgaatactc
      121 gtatccattg cgcggctggc cgaccgcgtc ctgctggaag gtgacggcct gctcgatggc
      181 cggcactacc gcgccggtgg tctcgtccgg ctcgaagccg gcatggacgg ccagcgtctc
      241 gaattggggt tcgcgctggt cgctcacctg ccggatgcta gcgggtgacg cgccccgccg
      301 atggagtggc tagcgggtgc gcgggaagcc gagatcgacc gaggacgtcc cggggtcggg
      361 ccaacgggcg gtcaccacct tggcccgggt gaagaactgg atgccctccg ggccgtacat
      421 gtggaggtca ccgaacagcg agctcttcca gccgccgaag ctgtagtagg caaccgggac
      481 cgggatcggg acattgatgc ccaccatccc gaccccgatg tcgaactgga actggcgggc
      541 cgccccgccg tcgcgggtga agatcgcggt gccgttggcg tatgggttgt cgtggatgat
      601 ctcgaccgca tcggtatacg tgtccgcgcg cacgacttcc agcaccgggc cgaagatctc
      661 gttctggtag cagtccatgt ccggccggac cttgtccagc agcgatacgc ccaggaagaa
      721 gccctgcccg tcctggtaca acggatgctc gcgaccgtcc accacgagct ccgcgccctg
      781 cgccgggccg ctctcgaggt aggacgcgac cttgtcgcgg tgctcgcggg tgatcagcgg
      841 ccccatctcg gcctcggggt ccgtgcctgg accgaccttg atcttgggca gtcgagcctg
      901 gatggcttcg accagcggct gggccacgtc ccccaccgcg acgaccacgc tgatcgccat
      961 gcaccgctcg ccggcggagc cataggcggc ggacacggcc gcatcggcgg ccatctcgat
     1021 gtccgcatcg gggagcacga tcatgtggtt cttcgcgccg cccagggcct ggacccgctt
     1081 gccggccttc gtcccgttct cgtagatgta tcgggcaata ggcgtggagc ccacgaagct
     1141 gaccgatgcg acgtgggggt gctcgaggat ccgatccact gccaccttgt cgccgtggac
     1201 cacgttgaac actccggctg gtaccccggc ctcggccagc agctccgcca tatagaccga
     1261 tgcggagggg tccttctccg aaggcttgag cacgaacgtg ttcccacagg cgatggcgtt
     1321 ggcgaacatc cacatcggga ccatggccgg gaagttgaac ggggtgatcc ccgcggccac
     1381 gcccagcggc tggcggatct ggtacacgtc gatcccggtc gacgcctgct cgctgaacga
     1441 gcccttcatc agctgcggga cgccagtggc gaactcgagg ttctcgaggc cgcgagcgac
     1501 ctcgcccagg gcgtccgagg ggactttgcc gtgctccgcg gtcaggtggg cggcgatatc
     1561 ggcccgatgc tggtcgacca ggttccggat ccggaacatg atctcggtcc ggcggctgag
     1621 ggaggtggcg cgccacgccg gaaaggcttc gtgcgccgcc ttgaccgcgg cgtcgacctc
     1681 ctcgacagac gcgaagtcca cctcgcgcgc gacctcgccg gtggccgggt tgtacaccgg
     1741 tccgtggcgg ccgctcgtgc cggcgacgcg cttgccgccg atccaatggt tgacccgcgg
     1801 caggtccgag ccctgtgccg atgccgactg tggcggttgg gcggtggtgg tcacgcgggc
     1861 agggtacccc tctgtgtgcg tcatcgctat tccgctctcg gggttgtctc tattactagc
     1921 gcgggtgtat tttagagacc gaacgcacgg ctgcgacgca tggggcgccg accgatgcga
     1981 gggatcgaaa gggtggcagc tcgatggcag accagagcct gtacgaccgt ctgggaggga
     2041 tcttcgcgat ctctgcggtg gtcgatcact tcagcgacgc gctgatcaag aacccgatcg
     2101 tcggccagca gtccgcgaat ccggccctgc ggcagtggca cagcgatcag ctcggccgcc
     2161 tgcccgggct gaagttcatg cggaccctgt gggttgccga ggtgagcggc ggtccgttca
     2221 cctaccaggg caccgagccg ggtcgcaccg ccctcgggct cgagcaggcg catcgcgagt
     2281 tcaagatcac gccggccgag ttcgacgagg tcgccgcgga gctcgctcgg accctggaca
     2341 ccgttggcgt gcccgagcag gagaagggtg aggtcctcgc cgccttcgcc gcgcacaagg
     2401 atgaggtcaa cgaaggatcc ctggccgcgg ccagctgagc cgggagttca ggcccgacgg
     2461 cggacgacca ccgtggggcg cgtgtcgcgc cagtcatagt cggctacagc ctcatcgggt
     2521 ccgatgaggc cggcctcgcg cagccacgcg tcgttgtaga gcttgccaag gtagttccgt
     2581 cccgaatcgg ggaggagaac cacgaacagc gcgttgggat cgtcaacgtc ccgggcgacc
     2641 tggagggcgg cccatagcgc agtcccaccc gactctccga cgagcatgcc ctcggccgcg
     2701 gtcgccgctc gggcggtcag gaacgcgtcg cgatcgggca cccggacgat gacatcggcg
     2761 acatcggtgt cataggtgtc gggccagaag tcctcgccga tgccctcggt caggtagggg
     2821 tggatcggtc ccccgctgaa gaccgagccg tcggtatcgg cgccgacgac caccaggtca
     2881 ggccgctgtt ggtggaggta tcggcccacg cctgagatgg tgcctccggt gcccacgccg
     2941 gccacgaaat gggtgatccg accctcggtc gcctcccaga tctcgggccc cgtggtcaag
     3001 tggtgggcct gcgggttggc cgagttggtg tactggtcgg gcttccaggc gccgggcgtc
     3061 tcgcgcgcca gccgatccga aacgcggtag tacgagcgct cgtcatcggg ttcgacctcg
     3121 gtggggcaga ccacgacctc ggccccgtag gcgcgcagca gggcacgctt ctcctccgac
     3181 tgcttgtcgg ccatgacgaa gatgcaccgg tagcccttca ccgcggcggc catggcca
//
LOCUS       DASYGU010000106        10002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_583428, whole genome shotgun sequence.
ACCESSION   DASYGU010000106 DASYGU010000000
VERSION     DASYGU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583428"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VGO32_02855"
     CDS             <1..467
                     /locus_tag="VGO32_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199723.1"
                     /translation="VAVGFGGYLLLNLFVYGDPFAFLQIQSEHWFKSFAWPWVGIGGV
                     VRRLGDPNLENVAVLGLAELATIGLGLVGTVYSLIHFRAAWTVWMAGSWVLFVSTAFV
                     LSVPRYAIAMFPLFAWFAVLAERRWLGIAIGIVSVLLLLWFSGRFALGSWAF"
     gene            455..1114
                     /locus_tag="VGO32_02860"
     CDS             455..1114
                     /locus_tag="VGO32_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199724.1"
                     /translation="MGLLMRPGRPARLGLVTVLALVAAAACGRPPPEGAVEVTLADGT
                     QVASWGDGEYGVVLIADDGETAADWAALATEIAANRMAVVALDSAAADPDTLAAAARW
                     LTDGGAERVATIASGDAGGTRLAEAAGDGAAVDQLILISGSLGDDQLAALGEPPKLFI
                     AAEGDEAGSLGAEQMTDAAAGAWNAVLLVPGSDRGAAILGGEGRDALIDGVVARLEER
                     R"
     gene            1317..1811
                     /locus_tag="VGO32_02865"
     CDS             1317..1811
                     /locus_tag="VGO32_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEV7199725.1"
                     /translation="MAASQRGPLEVIVEIPSGSRNKYEYDHENHRFVLDRVLYSSVHY
                     PCDYGFVDGTLADDGDPLDVLVVISEPTFPGCVVRARPVGVLDMRDDKGHDYKVLAVA
                     SDDPRWDETHTLGDLSPHRLREIENFFQIYKELEGRPVEVFGWLDADEAWRIIDESLV
                     AGQA"
     gene            1818..2963
                     /locus_tag="VGO32_02870"
     CDS             1818..2963
                     /locus_tag="VGO32_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7199726.1"
                     /translation="MDHAPRLGTGGRPLRLAYLGDPNSVHTRRWIGYFASHGHEVHLL
                     VGSHDTVAEGLPAAVMVHRFRREGRPRLPVLASLDVRRRLRGLLTEIDADVLHAHFLS
                     RYGWLGRVAGWHPYVVTLWGSDILITPWRSWRARIKGRLALVGADRITVASGHIGDAA
                     MRLGARPERIVRIHFGVDTDRFNPTRADPAVRGRLGLSGRIVFSPRALAPLYRHELVL
                     EAVAPMAEDVKVLVGRRRADAAYLDRLRARARELGMTDRFVVLDDVADEDLPALFAVA
                     DVVVSVPESDGLPLTVLEAMASAAPVVVSDLPGPRDPLLAHPRLIVPGGDAGALTTAL
                     DWVLGLAPAERDALGQALRAAVTGEYEYVRNMQAMEQVYRALVPDLV"
     gene            complement(2960..3856)
                     /locus_tag="VGO32_02875"
     CDS             complement(2960..3856)
                     /locus_tag="VGO32_02875"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7199727.1"
                     /translation="MLGASGFVGRHVLDVAAARTAEVTAVGRHDRRGDRSGRWMTGDL
                     AEASFARSLVGEVRPDVIINCAGVTSGSVEAMEQGNVTVVRNVLDAMREASAPPRLVH
                     LGSAVEYGAVERRMPMDESSPARPTGRYGESKAAATALVLGAPFADRMTVLRLFNVVG
                     GGAPRSSLLGSAIVKIRAAQAGDAGEIRLGDLSSHRDFVHVADVADAIVAAASATHIG
                     GLVLNVGSGVATRSRDLIRLLASVAGFAGEIVEVASAGPHGGHVPWQQADISRAAALL
                     DWRPAHTLQDAVRDAWEASAAG"
     gene            complement(3867..4607)
                     /locus_tag="VGO32_02880"
     CDS             complement(3867..4607)
                     /locus_tag="VGO32_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV7199728.1"
                     /translation="MPAVRHAVVLAGGLGTRLRPYTTSLPKPLVPIGGELPIVEIVLR
                     QLAARGFQRATLAIGHLGSLIQAVVGDGSRFGLEVDYAVEEQPLGTIGPILQILDRLP
                     EHFLVLNGDVLTDLDFGAVLAAHEGSGASLTITTFERVFETELGVLELDGDMVVGFRE
                     KPTLRFAVSMGVNGLSATTLSGYEPGTALGFDQLVLDLLGRGDLPRSFLHDGYWVDIG
                     RPEEYERANAEFAELRSVLLPAGVPGNG"
     gene            complement(4610..5596)
                     /locus_tag="VGO32_02885"
     CDS             complement(4610..5596)
                     /locus_tag="VGO32_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7199729.1"
                     /translation="MVAVTGAEGFIGSHLVERLVADGDRVRAMVQYNSFSARGWLDSL
                     GADVLAEVEIVAGDVRDSSSVLGFTDGAEVVYHLAALIAIPYSYRAPRSYVDTNVTGT
                     LNVLEAARAHQTPRVVHTSTSEVYGSAIRVPIDESHPLQAQSPYSASKIAADKLAEAY
                     VSSFSIPVVTLRPFNTYGPRQSTRAVIPTIVRQLAAGVTELRLGATAPIRDFTFVTDT
                     AAAFTAVGHAPANQVVGQVLNAGTGTGIAIGDLAHLIARLVDREVTVVSEGERMRPDA
                     SEVQELIADASALRDRTGWAPQLSLEDGLRVTCDWFAQPANLARYPSTEYTI"
     gene            5705..7165
                     /locus_tag="VGO32_02890"
     CDS             5705..7165
                     /locus_tag="VGO32_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV7199730.1"
                     /translation="MSRVIGRLVPDSAIYGLGGAANQTVAILLVPIYARQLGPAGVGV
                     AAVVNSTVALALMLVSLAIPQAFFRWFLSVAHDDRARSEVLSTTMGLRVVSSLLGAIV
                     VAIAVVPLTVLLYGSTEYVPVFLAIPVIVFFDSLNSIPLSYLRARRRARSYSIISFTR
                     AALGTVLILGFVVGSNLGVLGIVLGSGISAAVCAGIGLFVLRDADLRWGLNRDLARAM
                     LAFSLPLVPAAAAGWALNLSDRYILQGLTDADTVGVYALGYTGGMVVLAFVVQPFVLA
                     WAATSWEIAKEDDAERQYARVLTAFTVVAAFVGLAISALATDVLRLLVGPAFADSRLV
                     VPFSAFAYVLYGVYSVVAVGLNIRSQTRWVSLAMGIAAALSIGLNLLLIPLVGMLGAA
                     LATLVAYAVLAVTTAAFGQRYYPVPWDVTRVAGTMVAGMILAAAALLGPDLLLWRIAT
                     IAVFPLVLIATRIVSPDEIRAARRLIRRGGQDAPDS"
     gene            complement(7162..8319)
                     /locus_tag="VGO32_02895"
     CDS             complement(7162..8319)
                     /locus_tag="VGO32_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7199731.1"
                     /translation="MPNQATLVTMLLRNPFTADSRVEKEAASLTRAGYRVTVVAEGRP
                     GLPEQEERDGYAVVRVPRGPARLPGLRMREYRGRLVEAVVATRPDIVHAHDSDALDAI
                     ARVAERLRVPFVYDAHELWLGQENRGRSALYHGLFRAWYWWLQRRTVPRAAAVLTVSQ
                     PIAERLRKSYGVPVALVPNYPAEVGAVVRRDIRHLVTEPVPAGAPIVLYIGNIQLGRG
                     LEELVAALPECPGVVLVCLGAGRESGALPELADGLGVRDRIRFVSPVPADQVVSVAAS
                     ATIGVALTAPISENNRLALPNKLFQCMAAGIPVVATDAPHIRAIVEAAGAGVCVDPTS
                     PTAIASGLRRLLADSAEAEAMGRRGAAAVRDRFNWGQSEQALLEVYRRISG"
     gene            8347..9459
                     /locus_tag="VGO32_02900"
     CDS             8347..9459
                     /locus_tag="VGO32_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7199732.1"
                     /translation="MTSSEAAPSSWLVAGVPDAGSSGGSVRARHVFSALARRTGARVI
                     GAHGRRGLPALAVAVGSASGGWRRPLHVASTQLLPRRGLGLLRGKAQASVLDVHDHPR
                     LQAEGLGFSLDPTHRDALDRLFEVNAAAFDHLVVPSATFAELCRLPGDQVLVVSNGAD
                     TQLVRPAPAPAEPVVGMVSGAAPGRGIELLVEAMQLVRDEEPAATLRLALAATGPASA
                     AYLDSLRSALRDRAPWVGVQAVPYEELGAFLADTSLLVIPHPPNPYLDVATPVKLFDS
                     MAAGRPVVVTPRLETAAIVTATDAGVVANGDRADDLAEAILTLLRDAPRRDRLGASAR
                     RAAVAEYDWRVLAGRLADVLLARGPAAMRAGGPGTG"
     gene            complement(9456..>10002)
                     /locus_tag="VGO32_02905"
     CDS             complement(9456..>10002)
                     /locus_tag="VGO32_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV7199733.1"
                     /translation="LSEVAKSYMAGILAHARGMIAVLAPLVNSYKRLVPGYEAPVYIG
                     WARINRSALIRIPQISSGQLDSTRIELRCPDPSSNPYLAFAAMLAAGLDGVARKLPVP
                     DPVEENLYHLDEAKLASRKIRQLPGTLAEALDELAADEVIRDALGDHVFERFVEAKRE
                     EWDAYRMQVTGWETARYLEAF"
BASE COUNT         1394 a         3505 c         3512 g         1591 t
ORIGIN      
        1 tggtcgcggt gggcttcggc gggtacctgc tgctcaacct gttcgtgtat ggcgacccgt
       61 tcgccttcct ccagatccag agcgagcatt ggttcaagtc gttcgcctgg ccgtgggtcg
      121 ggatcggagg cgtcgtccgc cggctgggag atccaaatct cgagaacgtg gccgtgctcg
      181 ggttggccga gctcgcgacc atcggtctgg ggctggtggg aaccgtctat tcgctgatcc
      241 acttccgcgc ggcatggacg gtgtggatgg ccggcagctg ggtgctgttc gtgagcaccg
      301 cgttcgttct ctccgtgccg cgatacgcga tcgcgatgtt cccgctcttc gcctggttcg
      361 cggtgctcgc cgagcgacgg tggctgggca tcgcgatcgg gatcgtctcg gtcctcctgc
      421 tgctgtggtt ctcggggcgg ttcgcgctgg gctcgtgggc cttctgatgc ggcccggacg
      481 ccccgcgcgc ctgggtctcg tcaccgtcct cgccctcgtc gcggcggcgg cgtgcgggag
      541 gccacctccc gaaggcgcgg tggaggtgac cctggccgac ggaacgcagg ttgcatcgtg
      601 gggcgacggc gaatatggcg tcgtgctgat cgccgatgac ggagaaacgg cggccgactg
      661 ggcggcgctg gcgaccgaga tcgcggccaa ccggatggcg gtcgtggcgc tcgattcagc
      721 cgcggccgac cccgacacgc tggccgccgc ggctcgctgg ctgaccgatg gcggcgccga
      781 gcgggtggcg accatcgcct cgggcgacgc tggcgggaca cgcctcgccg aggccgccgg
      841 cgacggcgca gccgtcgatc agctcatcct catctcgggc agcctgggcg acgaccagct
      901 cgcggccctg ggcgagccgc ccaagctgtt catcgcggcc gagggggacg aggcgggatc
      961 gctgggggcc gagcagatga cggacgcagc ggccggtgcg tggaacgcgg tcctgctggt
     1021 gccgggctcg gaccgcggtg cggcgatcct gggaggcgaa ggccgcgacg cgctgatcga
     1081 tggcgtggtc gcccgactcg aggagcggcg ctaggtcgat ccgggccggg acaacgaagt
     1141 gtcccgaagg aaccgcggcg accgttgatg tcatctcggg tccggccagc tgccccttcg
     1201 ggacgttggc agaatggagc acgaatcggc tccgcgcaag cccccttttc cacgaccttt
     1261 tgcggttggc cgcgacttgt ccacaggctt tcggcggagc gggccatact gcgcccatgg
     1321 cagccagcca gcgcggcccg ctcgaggtca tcgtcgagat cccgtccggt agccggaaca
     1381 agtacgagta cgaccacgag aaccatcggt tcgtgctcga ccgggtcctg tacagcagcg
     1441 tccactatcc atgcgactac ggattcgtgg acgggaccct ggccgacgat ggggatccgc
     1501 tcgacgtcct ggtcgtcatc agcgagccca cgttccccgg ctgcgtcgtt cgcgcccggc
     1561 cagtcggggt gctggacatg cgcgacgaca agggacacga ctacaaggtc ctcgccgtcg
     1621 cctccgacga tccccgttgg gacgagacgc acaccctcgg cgacctctcc ccgcaccggt
     1681 tgagggagat cgagaacttc ttccagatct acaaggagct ggagggccgc ccggtggagg
     1741 tcttcggctg gctcgatgcc gatgaagcgt ggcggatcat cgacgaatcg ctggtggcgg
     1801 gacaggccta gagcggcgtg gaccacgctc cgcggctcgg gaccggcggg cggccgctgc
     1861 ggctggcgta cctgggtgac cccaacagcg tccacactcg gcgttggatc ggctacttcg
     1921 caagccatgg tcacgaggtg catttgctcg tcggttccca cgacactgtt gcggagggcc
     1981 tgccggcggc ggtcatggtc caccgatttc gtcgcgaagg caggccgcgg ctgccggtcc
     2041 tcgcctcgct cgacgttcgg cggcgtcttc gagggctcct gaccgagatc gacgcggacg
     2101 tccttcacgc ccacttcctg tcgcgatatg gctggctggg ccgcgtcgcg ggctggcatc
     2161 cctacgtcgt gaccctgtgg ggttccgaca tcctcatcac tccctggcgc tcatggcggg
     2221 cgcgaatcaa gggccgattg gccttggtcg gggccgatcg gatcacggtg gccagcggcc
     2281 atatcggcga cgcggcgatg cgtctgggcg cgcggccgga gcgcatcgtc cgcatccatt
     2341 tcggggtcga caccgatcgg ttcaacccga cccgggccga ccccgccgtg cgcggccgcc
     2401 tggggctgtc cggacggatc gtgttctcgc cacgggcgct ggcgccgctc taccgccacg
     2461 agctcgttct cgaggccgtg gccccgatgg ccgaggacgt gaaggtcctg gtgggccggc
     2521 gccgggcgga tgcggcgtac ctcgatcgcc tccgcgcgcg tgctcgggag ctgggcatga
     2581 ccgaccggtt cgtcgtcctg gacgacgtcg cggacgagga tctgcctgcc ctgttcgccg
     2641 ttgcggacgt ggtggtctcc gttccggaga gcgacggtct gccgctgacc gtgctcgagg
     2701 ccatggcctc ggcggctccg gtcgtcgtca gcgacctccc gggaccgcgg gacccgctcc
     2761 tggcgcaccc ccgcctcatc gtcccgggag gtgacgccgg ggcactgacc acggcgctgg
     2821 actgggtgct cggtctcgcg cccgccgaac gcgacgccct cgggcaggcg ctgcgggccg
     2881 cggtcaccgg tgagtacgag tacgtccgga atatgcaggc catggagcag gtgtaccgag
     2941 cgctggttcc ggacctggtc tagcccgctg ccgatgcctc ccaggcatcg cgaacggcat
     3001 cctggagggt gtgggctggg cgccagtcga ggagcgcggc agcccggctg atatcggcct
     3061 gttgccacgg gacgtgccct ccatgaggcc ccgcgctcgc gacctcgacg atctcccccg
     3121 cgaacccggc gacggaggcc agcagcctga tgaggtcacg tgaccgcgtc gccacgccgg
     3181 agcccacgtt caggaccagc ccgccgatat gcgtggcgga tgcggcggca acaatggcgt
     3241 ccgcgacgtc cgccacgtga acgaagtctc gatggctgga caggtcaccc agccggatct
     3301 cgccggcatc gccggcctgc gccgcccgga tcttgacgat cgccgacccg agcaggctgc
     3361 tgcggggcgc tcctccaccc acgacattga acagcctcag cacggtcatc cggtccgcga
     3421 aaggcgctcc gagcaccagc gcggtcgcgg cggccttcga ttcgccatat cggccggtcg
     3481 gtcgcgcagg actcgattcg tccatcggca tccgccgctc caccgcgcca tattcgacgg
     3541 cggaaccaag gtggaccagc ctgggcggcg cgctcgcctc gcgcattgcg tcgagcacgt
     3601 tccggacgac ggtgacgttg ccctgctcca tggcttccac cgacccagac gtgacgcctg
     3661 cacagttgat gatcacgtcc ggccggacct cgccgaccag cgaccgagcg aaggaggcct
     3721 cggccagatc cccggtcatc caccgcccgc tgcggtcccc acgccggtcg tgccgaccga
     3781 ccgcggtgac ttcggccgtc cgtgccgccg cgacgtcgag gacatggcgg ccgacgaagc
     3841 cggaggcccc gagcacgagg atccgctcag ccgtttccgg gcacccctgc gggcaggagc
     3901 acggatcgca gctccgcgaa ctcggcattg gcccgctcgt actcctccgg tcgcccgatg
     3961 tcgacccagt agccgtcgtg caggaagctt cgtggcaggt cgccgcggcc gaggaggtcc
     4021 aggaccagct gatcgaagcc gagggcggtt cccggctcgt agcccgacag ggtcgtggcg
     4081 ctcagaccgt tgacgcccat gctgaccgcg aagcgcaggg tcggcttctc gcggaagccg
     4141 acgaccatat cgccgtcgag ctcgaggacg ccgagctcgg tctcgaagac gcgctcgaac
     4201 gtcgtgatcg tgagcgacgc cccggagccc tcgtgcgcgg ccaggacggc gccgaagtcg
     4261 agatcggtca ggacgtcgcc gttcaggacg aggaagtgct ccggcaggcg gtccaggatc
     4321 tggaggatgg gaccgatcgt tccgaggggc tgctcctcca ccgcgtagtc gacttcgagc
     4381 ccgaagcggg acccgtcccc gacgacggcc tggatgaggc tgccgaggtg gccgatggcc
     4441 agcgtcgcgc gctggaagcc ccgcgccgcg agctggcgga ggacgatctc cacgatcggg
     4501 agctcccctc cgatcggaac cagcggcttg gggagggagg tggtgtacgg tcggagccgc
     4561 gtccccagac cgccggccag gacgaccgcg tggcgcacgg caggcatggt cagatcgtgt
     4621 actcggtcga cggatatcgt gcgaggttcg ccggctgtgc gaaccagtcg cacgtgacac
     4681 gaagcccatc ctcgagactg agctgcggcg cccatccggt ccgatcgcgg agcgcggacg
     4741 cgtccgcgat cagctcctgc acctccgaag cgtcgggccg catccgctcg ccctccgata
     4801 cgacggtgac ctcgcgatcc acgaggcgcg cgatgaggtg ggcgaggtcg ccgatcgcta
     4861 taccggtgcc ggtcccggcg ttcaggacct ggcccacgac ctggttggcg ggcgcgtgcc
     4921 ccaccgccgt gaaggcggcc gccgtgtcgg tcacgaacgt gaagtcgcga atcggagccg
     4981 tggcaccgag cctgagctcc gtgaccccgg cagcaagctg gcgaacgatg gtcgggatga
     5041 cggcacgcgt tgactgccga gggccgtagg tgttgaacgg gcggagggtc acgaccggga
     5101 tcgagaagct gctgacgtag gcctcggcca gcttgtcagc cgcaatcttc gaggcggagt
     5161 acggcgactg ggcctgcagc ggatgggact cgtcgatcgg gacccggatc gcggagccgt
     5221 agacctcgct ggtcgaggtg tgcaccaccc gaggtgtctg gtgcgcgcgc gccgcctcga
     5281 gcacgttgag ggtcccggtc acgttggtat ccacgtacga gcggggagcg cggtaggaat
     5341 acggaatcgc gatcagcgcg gcgaggtggt agacgacctc cgccccatcg gtgaatccga
     5401 gaaccgacga gctgtcgcgc acgtcaccgg cgacgatctc gacctcggcg agcacgtcgg
     5461 cgcccaggct atcgagccat ccgcgggcgc tgaacgagtt gtactggacc atcgcccgga
     5521 cccggtcacc atcggcgacc aggcgttcga ccagatgcga cccgatgaaa ccctcggcgc
     5581 ccgtgaccgc gaccagcgtg cccagatgtc cagcctcctg acggtggatt gaaccggaaa
     5641 ctaagtatcg cccagatatc ggtcgaggag gtccatacaa tcccgtcgtg agctgcaccc
     5701 ttccgtgagc cgcgtcatcg ggcggctggt cccggactcc gccatctacg ggctcggcgg
     5761 ggcagcgaat cagacggtgg cgatcctcct ggtcccgatc tatgcccgcc aactcggccc
     5821 cgcgggcgtc ggtgtcgccg cggtcgtcaa ctccacggtg gcattggccc tgatgctcgt
     5881 cagcctcgcc atcccgcagg cgttcttccg gtggttcctg agcgtggcgc acgacgatcg
     5941 agcgcgcagc gaggtcctga gcacgacgat gggcctgcgc gtcgtctctt ccctcctcgg
     6001 cgcgatcgtg gtcgcgatcg ccgtcgtgcc actgaccgta ctcctgtacg gcagcacgga
     6061 gtacgtaccg gtcttcctgg cgatcccggt catcgtcttc ttcgactcgc tgaacagcat
     6121 cccgctgtcc tatctccgcg cccggcgccg tgcccgcagc tactcgatca tcagcttcac
     6181 ccgggctgcg ctcggcaccg ttctgatcct cggtttcgtc gtcggatcga acctcggggt
     6241 cctgggcatc gtcctcggtt ccggcatctc ggctgccgtc tgcgccggga tcggcctgtt
     6301 cgtcctccgg gacgcggacc tgcgatgggg cctgaaccgt gacctggcgc gcgcgatgct
     6361 tgccttctct ctcccgctcg tcccggccgc cgcagccggc tgggcgctga acctgtccga
     6421 ccggtacatc ctccaggggt tgaccgatgc cgacacggtt ggcgtctacg ccctcggcta
     6481 caccggcggc atggtcgtgc tcgccttcgt cgtccagccg ttcgtgctgg cctgggcggc
     6541 aacctcgtgg gagatcgcga aagaggacga tgcggaacgc cagtacgccc gggtcctgac
     6601 cgcattcacc gtggtcgccg ccttcgtcgg cctcgccatc tcggccctcg cgaccgatgt
     6661 cctccggctg ctcgtcggcc cggccttcgc cgacagccgg ctggtcgtgc cgttcagcgc
     6721 cttcgcctac gtcctgtacg gcgtgtactc ggtcgtcgcg gtcgggctca acatccggtc
     6781 acagacacgc tgggtctcgc tggccatggg catcgcggcg gcgctcagca ttgggctcaa
     6841 cctgctgctc atccccctcg tcgggatgtt gggcgcggcc ctggcgaccc tcgttgccta
     6901 cgcggtgctc gccgtcacga cggcggcatt cggccagcgc tattacccgg ttccgtggga
     6961 cgtcacacga gtggcgggga ccatggtcgc cggcatgatc ctggctgccg ccgcgctcct
     7021 cggtcccgac ctgctcctgt ggcggatcgc caccattgcc gtgttcccgc tcgtcctgat
     7081 cgcgacccgg atcgtatccc cggacgagat ccgggccgcg cggcggctca ttcgccgcgg
     7141 gggccaggac gcgccggaca gctaaccgct gatccggcgg tatacctcga gcagggcctg
     7201 ttccgactgt ccccagttga accggtctcg gaccgcggcc gctccccggc gacccatcgc
     7261 ctccgcctcg gccgagtccg cgaggagccg gcgcagccct gacgcaatgg ccgtcgggct
     7321 cgtcggatcg acgcagacgc ccgcaccggc cgcttcaacg atcgcgcgga tgtggggcgc
     7381 gtcggtggcg accaccggga tgccggcggc catgcattgg aacagcttgt tcggcagggc
     7441 gaggcgattg ttctccgaga tgggcgccgt caaggccacg ccgatggtgg cggacgccgc
     7501 gacggacacc acctggtcgg cgggcaccgg cgacacgaac cggatgcgat cgcggacacc
     7561 gagcccgtcg gccagctcgg gcagggcgcc cgattctcga ccggcgccga gacagacaag
     7621 gacgaccccc ggacattcgg ggagggcagc gacgagctcc tccagcccgc gtccgagttg
     7681 aatattgccg atatagagga cgatcggtgc gccagcggga acgggctcgg tgacgaggtg
     7741 ccggatgtcg cggcggacca cggccccgac ctcggcggga tagttcggga ccagggccac
     7801 cggcacccca taggacttcc tgagtcgctc ggcaatcggc tggctgacgg tcaggaccgc
     7861 ggcggcccgg ggcacggtgc gtcgctggag ccaccagtac cacgccctga acaggccgtg
     7921 gtagagcgcg ctccgcccgc ggttttcctg ccccagccac agctcgtggg cgtcgtacac
     7981 gaacgggacg cggagccgct ccgcgaccct cgcgatggcg tccagggcat cgctgtcgtg
     8041 agcgtggacg atgtccggtc gggtggcgac cacggcctcg accagccggc ctcggtattc
     8101 gcgcatccga agaccgggca ggcgagctgg gccgcgcgga acgcgcacga ccgcatatcc
     8161 atcgcgctcc tcctgctcgg gcagtccggg tcggccctct gccacgaccg tcacccgata
     8221 gccagcccgg gtcaggctgg cggcctcctt ctctacccga gagtcggcgg tgaatgggtt
     8281 tcggagcagc atcgtgacca gcgtggcctg attgggcatc ggccggcaag cataatcggg
     8341 agccggatga catcgagcga agccgcgccc tcgagctggc tcgtcgcggg agtgccggac
     8401 gccggctcca gcggcggatc ggtccgcgcc cggcacgtct tctcggccct ggcccgacgg
     8461 accggtgctc gggtcatcgg agcccacggc cggcgcggcc tgcctgccct ggcggtggcc
     8521 gtcggatcgg catccggcgg ctggcgtcgg ccgttgcacg tggcctccac ccagctgctg
     8581 ccgcgacgtg gtttgggcct cctgcgcggc aaggcgcagg catcggtcct ggacgtccac
     8641 gaccatcccc ggctccaggc ggaggggctg ggattcagcc tggaccccac ccaccgcgat
     8701 gcgctcgacc gtctgttcga ggtcaacgcc gccgccttcg accacctggt cgtgccgtcc
     8761 gccacgttcg ccgagctctg tcgtctgccg ggggaccagg tcctcgtcgt ctccaacggt
     8821 gcggacacgc agctcgtgcg ccccgcgccg gcgccggccg agcccgtggt ggggatggtg
     8881 tccggggcgg ctcccggaag aggcatcgaa ctcctcgtcg aggccatgca gctcgtccgc
     8941 gacgaggagc cggccgccac cctgcgcctg gcgttggctg ccaccggacc ggcgagcgct
     9001 gcctacctcg actcgctccg ctcagcgctg cgggaccgcg cgccatgggt cggcgtgcag
     9061 gccgtcccgt acgaggagct gggagcattc ctcgcggaca cgtcgctcct cgtcatcccc
     9121 catccgccga acccgtacct ggatgtggcc accccggtca agctcttcga ctcaatggcg
     9181 gccgggcggc cagtggtcgt caccccgcgg ctcgagacgg cggcgattgt gaccgcgacc
     9241 gacgccgggg tggtggccaa tggggatcgc gcggatgacc tggcggaggc catcctcacc
     9301 ctcctccgcg atgcgccccg ccgcgatcgg ctcggcgcga gcgctcgcag ggcggcggtc
     9361 gcagagtacg actggcgcgt cctggccggc cggctggcag acgtcctcct cgcccgtgga
     9421 ccggctgcga tgcgagccgg gggaccgggg accggctaga acgcctcgag gtagcgggcc
     9481 gtctcccagc cggtgacctg catccggtag gcgtcccact cctcgcgctt ggcctccacg
     9541 aaccgctcga agacgtggtc gccgagcgca tcacggatga cctcgtcggc cgcgagctcg
     9601 tcgagtgcct cggccagcgt cccgggcagc tgccggatct tccggctggc gagcttcgcc
     9661 tcgtcgaggt ggtacaggtt ctcctcgacc gggtcgggaa cgggcagctt gcgggccacg
     9721 ccgtccaggc cggcggcgag catggccgcg aaggcgagat acggattgga tgacgggtcg
     9781 gggcagcgga gctcgatccg ggtcgagtcc agctggccgc tcgagatctg cgggatgcgg
     9841 atcagcgctg atcggttgat ccgcgcccag ccgatgtaca ccggcgcctc gtatcccggc
     9901 accagccgct tgtagctgtt cacgagcggg gcgaggacgg cgatcatgcc ccgcgcgtgg
     9961 gccaggatcc cggccatgta cgacttggcc acctcggaca gg
//
LOCUS       DASYGU010000107         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1200672, whole genome shotgun sequence.
ACCESSION   DASYGU010000107 DASYGU010000000
VERSION     DASYGU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200672"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VGO32_02910"
     CDS             <1..746
                     /locus_tag="VGO32_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7199734.1"
                     /translation="KNHMIVLPDADIEMAADAAVSAAYGSAGERCMAISVVVAVGDVA
                     EPLVDAIQKRLPRIKVGPGTDPEAEMGPLITREHRDKVASYLESGPAQGADLVVDGRE
                     HPLYQDGQGFFLGVSLLDKVRPDMDCYQNEIFGPVLEVVRADTYTDAVEIIHDNPYAN
                     GTAIFTRDGGAARQFQFDIGVGMVGINVPIPVPVAYYSFGGWKSSLFGDLHMYGPEGI
                     QFFTRAKVVTARWPDPGTSSVDLGFPRTR"
     gene            789..1955
                     /locus_tag="VGO32_02915"
     CDS             789..1955
                     /locus_tag="VGO32_02915"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV7199735.1"
                     /translation="MSDQREPQFETLAVHAGFEPDETTGAVVPAIEQAVTFQQDAVGQ
                     PRNGYEYSRTGNPTRQLLERAVAELEGGTHGIAYASGLAATQNLLYLLEPGQRILLGD
                     DVYGGTWRLAEKVWSRYGIQVDVADLRDLDAVAFALTDKTAMIWLESPTNPMLKVVDI
                     RAIVRLAATSGALTVVDNTFATPFLQRPLDFGADVVLHSATKYLGGHSDVVSGVVVTR
                     NDEVAEKLRYHQNAAGAVPSPFDCWLVLRGLRTLAIRMDRASANAGIIADWLTKRPEV
                     TAVHYPGLPDHPQEVLVRRQMRLPGAMLSFQVRGGALAANRIAGATRIFTLAESLGAV
                     ESLIEVPAVMTHASVAGSPLEVPADLIRLSVGIEAAEDLVADLEQALAAGATAD"
     gene            1972..2517
                     /locus_tag="VGO32_02920"
     CDS             1972..2517
                     /locus_tag="VGO32_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199736.1"
                     /translation="MAADEPRTPEPEPVREPTPDGPTELVETDDGELVERLSFGTATL
                     LLVWLFIVGAGTALLAAWGIAALVLGQRLGPAQLFGEFGFYVALYGATGPTVLWLTGR
                     AQGHGWPWFVATAIRIGLFMSGLTIAFGALVTLVLGNGITVGIAILGLTVLLATFVIS
                     ILWGLATWAADRWIAQARVST"
BASE COUNT          364 a          917 c          874 g          388 t
ORIGIN      
        1 cgaagaacca catgatcgtg ctccccgatg cggacatcga gatggcggcc gatgcggccg
       61 tgtccgccgc ctacggctcc gccggcgagc ggtgcatggc gatcagcgtg gtcgtcgcgg
      121 tcggcgacgt ggccgagccc ctcgtggacg caatccagaa gcgcctcccc cgaatcaagg
      181 tcggtcccgg cacggacccc gaggccgaga tggggccgct catcacccgc gagcaccgcg
      241 acaaggtcgc gagctacctc gagagcggcc cggcgcaggg cgcggacctg gtggtggacg
      301 gtcgcgagca cccgctctac caggacggcc aggggttctt cctgggggtc tcgctgctcg
      361 acaaggtgcg acccgacatg gactgctacc agaacgagat cttcggcccg gtgctggaag
      421 tcgtccgcgc ggacacctac accgatgcgg tcgagatcat ccatgacaac ccgtacgcca
      481 acggcaccgc gatcttcacc cgcgacggcg gggcggcccg ccagttccag ttcgacatcg
      541 gggtcggcat ggtgggcatc aatgttccga ttccggtccc ggtggcgtac tacagcttcg
      601 gcggctggaa gagctcgctg ttcggtgacc tccacatgta cggcccggag ggcatccagt
      661 tcttcacccg ggccaaggtg gtcaccgccc gttggcccga ccccgggacg tcctcggtcg
      721 atctcggctt cccgcgcacc cgctagccgc tccatcggcg gggcgcgcca cccgctagca
      781 tccggcaggt gagcgaccag cgcgaacccc aattcgagac gctggccgtc catgccggct
      841 tcgagccgga cgagaccacc ggcgcggtgg tgccggcgat cgagcaggcc gtcaccttcc
      901 agcaggacgc ggtcggccag ccgcgcaacg gatacgagta ttcgcgcacc ggcaacccga
      961 cccgccagct gctcgagcgc gcggtcgccg agctcgaggg cggcacgcac gggatcgcct
     1021 atgccagcgg cctggcggcc acccagaacc tgctgtacct cctcgagccc ggccagcgga
     1081 tcctgctcgg cgacgacgtg tacggcggca cctggcggct ggccgagaag gtctggagcc
     1141 ggtacgggat ccaggtggac gtggccgacc tccgcgacct ggacgcggtg gccttcgcgc
     1201 tcacggacaa gacggccatg atctggctcg aaagcccgac caacccgatg ctcaaggtgg
     1261 tcgacatccg ggccatcgtc cgtctggcgg ccacctccgg cgccctgacc gtggtcgaca
     1321 acaccttcgc cacccccttc ctccagcggc cgctcgactt cggggccgac gtcgtcctcc
     1381 actccgccac gaagtacctg ggtggccact cggacgtggt gagcggcgtg gtcgtgaccc
     1441 gcaacgacga ggtcgccgag aagctccgct atcaccagaa cgccgccggt gcggtgccca
     1501 gcccgttcga ctgctggctc gtcctgcgcg gcctccgaac gctggccatc cggatggatc
     1561 gggcctcggc caacgccggg atcatcgcgg actggctcac gaaacggccg gaggtcacgg
     1621 ccgtccacta tcccgggctg cccgaccacc cacaggaggt cctcgtccgg cgccagatgc
     1681 ggctcccggg cgcgatgctc tccttccagg tccgtggtgg agcgttggcc gccaaccgga
     1741 tcgccggcgc gacccggatc ttcaccctcg ccgagtcgct cggcgcggtg gaatccctga
     1801 tcgaggtgcc ggcggtcatg acccacgcct cggtcgctgg ctcgcccctc gaggtgccgg
     1861 cggacctcat ccggctgtcg gtgggcatcg aggccgccga ggacctggtg gccgacctcg
     1921 agcaggcact ggcggccggc gcgaccgcgg actgatcggc ggatctgacc gatggcggct
     1981 gacgagccga ggaccccgga accggagccc gttcgcgagc cgaccccgga cggaccgacc
     2041 gagctggtcg agaccgatga cggggagctg gtcgagcgcc tctcgtttgg tacggccacc
     2101 ctgctcctgg tgtggctgtt catcgtcggt gccggcaccg cgctgctcgc ggcgtggggg
     2161 atcgccgcgc tcgtccttgg tcagcgcctg ggtcccgcgc agctcttcgg cgagttcggc
     2221 ttctacgtcg ccctgtacgg ggccaccggc ccaaccgtgc tgtggctgac cggtcgtgcg
     2281 cagggccacg gctggccgtg gttcgtggcc accgcgatcc gcatcggcct gttcatgtcc
     2341 ggcctgacga ttgcgttcgg agcactggtg accctggtct tgggcaacgg gatcacggtg
     2401 gggatcgcga tcctgggcct caccgtgctg ctggccacct tcgtgatcag catcctgtgg
     2461 gggttggcca cgtgggctgc tgaccgctgg atcgcccagg cccgggtctc aacgtagcgg
     2521 cggcgggcgg cccggagccg ccc
//
LOCUS       DASYGU010000108         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1612942, whole genome shotgun sequence.
ACCESSION   DASYGU010000108 DASYGU010000000
VERSION     DASYGU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612942"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VGO32_02925"
     CDS             <1..1006
                     /locus_tag="VGO32_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEV7199737.1"
                     /translation="IAATAVGASEVILAVRRDWTAAIERLKGAVAEAEAAHLAGYLIL
                     GTDLSVQASVWEGSGAYVAGEETALINALQGDRGMPLIRPPYPAERGLWGVPTIVHNA
                     ETLAHAAWILGHSAAQFARVGPKSAPGTKLVTIAGAVRTPGLAEVPLGTPITEIIDLA
                     GGPSGTTKAAFIGGPGAGALAADELGSVGYGFETLAEAGGHIGSGSVLVTDAATCMVA
                     TARFLLDFSAREACGKAVPCRIGTKRLVEALDRILAATPRPNDLVLLRELSVKIADTA
                     LCRLESLAPRPMLTTLDRFADEYRAHAERGVCLAGSCSTDAVAPLLTPLAEAGGLRAW
                     "
     gene            1023..>2152
                     /locus_tag="VGO32_02930"
     CDS             1023..>2152
                     /locus_tag="VGO32_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV7199738.1"
                     /translation="MTFIDNENGKGTGNGNGHGQGKLEDYAPIWPVLPGTTPVTVTIN
                     GRQVRAEEGQTILQACRVAGIEIPTLCYEPKLSPYGACRICVVEVEGQENTPISCGTE
                     IADGMVITTHSERVIENRRLVLELIFSDHDAYCLPPCQFKCPTRVDIPGYLKANSEGN
                     WEEATRILKRSLPFPAILGRVCPAPCETHCRREEVDTAIAIRDSHRYCADRVLEVDAP
                     APLPWPKESDTGRRVAIVGSGPAGMTVAYYLQLRGHHCDVFEADPEQGGMLRYGIPEY
                     RLPKGWMDRELNHVWELGATFKPNMRLGRDFHLADLVDQGYDAIFVGIGCYKPNEMGI
                     PNEDADGTVNALENLYNSTRGIPVPQLKGARVVVIGGGFTAI"
BASE COUNT          334 a          772 c          745 g          301 t
ORIGIN      
        1 gatcgccgcc accgcggtcg gggcatcgga ggtgatcctc gccgtccgtc gcgactggac
       61 cgcggccatc gaacgcctca agggagcggt ggccgaggcg gaggccgccc acctggccgg
      121 ctacctcatc ctgggcaccg acctctcggt ccaggcatcg gtctgggagg ggtcaggggc
      181 ctacgtcgcg ggcgaggaaa ccgcgctcat caacgccctg cagggcgatc ggggcatgcc
      241 cctcatccgg ccgccctacc cggccgagcg cgggctgtgg ggcgtgccga ccatcgtcca
      301 caacgccgag accctggccc atgcggcctg gatcctgggt cacagtgcgg cccagttcgc
      361 gagggtggga cccaagtctg cacccgggac caagctggtg accatcgccg gcgcggtcag
      421 gacgccgggc ctggccgagg tcccgctcgg cacgccgatc acggagatca tcgatctcgc
      481 cggtggaccg tcggggacga ccaaggccgc gttcatcggc ggaccgggag ccggcgccct
      541 cgccgccgac gagctcggat cggtcggcta cgggttcgag acgctagctg aggcgggcgg
      601 tcacatcggc tcgggatcgg tgctggtcac cgatgccgcc acgtgcatgg tggccactgc
      661 tcgcttcctc ctagacttct cggcccgcga ggcatgcgga aaggccgtcc cgtgccggat
      721 cggtaccaag cgcctggtgg aggccctcga ccggatcctg gccgccacgc cccggccgaa
      781 cgacctcgtc ctcctgcgcg agctgtcggt gaagatcgcg gacaccgccc tgtgccggct
      841 cgagtcgctc gcgccgcggc ccatgctcac gaccctggac cgcttcgccg acgagtaccg
      901 ggcccatgcc gagcgcggcg tgtgcctcgc cggctcctgc tcgaccgatg ccgtggcccc
      961 gctgctgacc ccgctggcgg aagccggcgg gctgcgcgcc tggtgacggt aggattgggc
     1021 cgatgacgtt catcgacaac gagaacggca agggcaccgg caatggcaac ggccatggcc
     1081 agggaaagct cgaggactac gcccccatct ggccggtcct gcccggcacg acaccggtca
     1141 cggtcacgat caacggccgc caggtccgcg ccgaggaggg gcagaccatc ctccaggcct
     1201 gccgggtagc cggcatcgag atcccgaccc tgtgctacga gcccaagctg tccccgtacg
     1261 gtgcgtgccg gatctgcgtg gtcgaggtcg agggccagga gaacaccccc atctcgtgcg
     1321 ggaccgagat cgccgacggg atggtcatca ccacccattc cgagcgggtc atcgagaacc
     1381 ggcgcctcgt cctggagctg atcttctcgg accacgacgc gtactgcctc ccgccctgtc
     1441 agttcaagtg cccgacccgg gtggacatcc ccggctacct gaaggcgaac tcggagggca
     1501 actgggagga ggcgacccgg atcctcaagc ggtcgctccc cttcccggcc atcctcggcc
     1561 gggtctgccc ggccccgtgc gagacgcact gccggcgcga ggaggtggac accgccatcg
     1621 ccatccgcga ctcgcatcgg tactgcgccg accgcgtgct cgaggtggac gcacccgcgc
     1681 ccctgccgtg gcccaaggag tcggacaccg ggcgacgggt cgcgatcgtg ggctccggcc
     1741 cggcgggcat gacggtcgcc tactacctcc agcttcgcgg ccaccactgc gacgtgttcg
     1801 aggccgatcc cgagcagggc ggcatgctcc gttacggcat cccggagtac cgcctgccca
     1861 agggctggat ggaccgggag ctgaaccacg tgtgggagct gggcgcgacc ttcaagccga
     1921 acatgcgcct cggtcgggac ttccacctcg ccgacctggt ggaccagggc tacgacgcga
     1981 tcttcgtggg gatcggttgc tacaagccga acgagatggg gatcccaaac gaggacgcgg
     2041 acgggacggt caacgcgctc gagaacctgt acaacagcac ccgagggatc ccggtcccgc
     2101 agctcaaggg cgcgcgggtg gtggtcatcg gcggcggctt cacggccatc ga
//
LOCUS       DASYGU010000109         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_533022, whole genome shotgun sequence.
ACCESSION   DASYGU010000109 DASYGU010000000
VERSION     DASYGU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533022"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VGO32_02935"
     CDS             <1..833
                     /locus_tag="VGO32_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7199739.1"
                     /translation="PVDRYGQVEPAAVAEAIGPHTTMAVIGYANNEVGTIQPVAEIGA
                     ICRERGIRLYVDATQAAAALPLDVDALQVDLLGISAHKIEGPKGVGALFVRQGTNVIP
                     QQVGGSQERQRRAGTENVAGAVGLAAALRLVRDDPAAMATESDRQRVLRDGLLSRLQT
                     IGDGQLTGHPTARLPNSVSWVFEGVEGGDLVAALDLDGLAVSTGSACTSGSAEPSHVL
                     AAMGIPPERIRGSLRITLGRMTTASETEQAADLVTAAVARIRAAAAVPRETSAAPAGA
                     "
     gene            839..1924
                     /gene="mnmA"
                     /locus_tag="VGO32_02940"
     CDS             839..1924
                     /gene="mnmA"
                     /locus_tag="VGO32_02940"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV7199740.1"
                     /translation="MTRIVAAMSGGVDSSVAAALLARDARAGGEEVVGVWMRTHPDRG
                     EGWEPRRGCCSTEAADDARRVADRIGIPFFILNVEAEFGAQVIDAFADAYLDGTTPNP
                     CQACNQNIKFDLLARRAVGAYGADGVATGHYARVQERDGAWRLLRAHDEAKDQTYFLW
                     NLDQRQLAATRFPLGEMTKPEVRQLAADLELPTAATPESQEICFVPAGDYRALLAERR
                     AYEGEAGPIVDREGQRVGTHTGYAHYTIGQRRGLGLATGEARYVTEIRPDTNEVVVGD
                     RDALATRTVVADARHFVAGAAPADRFEAQVRIRHRSPDVACEVTLVGADRLLIETAEP
                     VWGPAPGQSAVLYRDDECLGGGRIART"
     gene            1926..2231
                     /locus_tag="VGO32_02945"
     CDS             1926..2231
                     /locus_tag="VGO32_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199741.1"
                     /translation="MVPLALWTLLVSAINLTLFVAIRGRWGRELPFLVIAAVIGTFAG
                     NFLAALLEVDVLVLGEFHLVGAVIGAQLALITTAAVATLLAGTRGSTGRRDGAGSGR"
BASE COUNT          311 a          801 c          817 g          307 t
ORIGIN      
        1 tcccggtcga ccgatatgga caggtcgagc cggcggccgt ggctgaagcc atcggtccgc
       61 acaccaccat ggccgtcatc ggctacgcca acaacgaggt gggcaccatc cagcctgtgg
      121 ccgagatcgg cgccatctgc cgcgagcgtg ggatccggct gtacgtggat gccacccagg
      181 ccgcggccgc gctgccgctg gacgtggatg cgctccaggt cgacctgctg ggcatctcgg
      241 cgcacaagat cgagggcccc aagggagtcg gcgcgctgtt cgtccggcag gggacgaacg
      301 tcatcccgca gcaggtcggt ggttcgcagg agcggcagcg acgggccggc accgagaacg
      361 tggccggggc ggtggggctg gccgcggcgc tccgcctggt ccgcgacgat ccggccgcca
      421 tggccaccga gtccgatcgc cagcgcgtcc tccgcgatgg actccttagt cgattgcaaa
      481 cgatcggtga cgggcagctg accgggcacc ccaccgctcg gctgccgaac agcgtgagct
      541 gggtgttcga aggcgttgag ggaggcgact tggtggccgc ccttgacctc gacggcctgg
      601 cggtatcgac cggcagcgcc tgcacgtcgg gcagcgccga gccgagccac gtcctggccg
      661 cgatgggcat ccctcccgag cgcattcgcg gctcgctgcg gatcacgctg gggcggatga
      721 cgaccgcgag cgagaccgag caggcggccg acctggtgac ggccgccgtg gcccgcatcc
      781 gggcggcggc tgcggtcccg cgcgagacct ccgcggcccc ggcgggggca tagaagccat
      841 gacccggatc gtcgccgcca tgagcggcgg ggtggactcg agcgtggcgg cggccctgct
      901 cgcccgcgac gcgcgggccg gtggggagga ggtggtcggc gtctggatgc ggacccatcc
      961 cgaccggggc gaaggctggg agccgcgccg cggctgctgc tcgaccgagg ccgcggacga
     1021 cgcccgtcgg gtcgccgatc ggatcggcat cccgttcttc atcctgaacg tcgaggccga
     1081 gttcggcgcc caggtcatcg acgccttcgc cgatgcctac ctcgacggca cgacccccaa
     1141 tccgtgccag gcgtgcaacc agaacatcaa gttcgatctc ctcgcccggc gggcggtggg
     1201 cgcctatggg gccgacggcg tggcgaccgg ccactacgcc cgggtccagg aacgcgacgg
     1261 ggcatggcga ctcctgcgcg cgcacgacga agcgaaggac cagacgtact tcctgtggaa
     1321 cctcgaccag cggcagctgg ccgccacccg ctttccactg ggggagatga ccaagccgga
     1381 ggtccgccag ctggccgccg atctcgagct gcccaccgcg gccacgcccg agtcgcagga
     1441 gatctgcttc gtcccggccg gcgactaccg cgccctgctc gccgagcggc gcgcctacga
     1501 aggcgaggcc ggcccgatcg tggaccgcga gggccagcgc gtcggcaccc acaccggata
     1561 cgcccactac acgatcggcc aacggcgcgg gcttggactg gcgaccggcg aggcgcgata
     1621 cgtgaccgag attcggccgg acacgaacga ggtcgtggtc ggggaccgcg atgcgctcgc
     1681 cacgcggacc gtggtcgccg atgcgcgcca cttcgtcgcc ggggcggcgc ccgccgaccg
     1741 cttcgaggcc caggtccgga tccgtcatcg gtcacccgat gtcgcctgtg aggtgaccct
     1801 ggtcggggcc gatcggctcc tgatcgagac cgccgagccg gtatgggggc cggcgccggg
     1861 ccagtccgcc gtcctctacc gcgacgacga gtgcctgggc gggggccgga tcgcccgtac
     1921 ctgacgtggt gccgctggcc ctctggaccc tgctggtttc cgccatcaac ctgaccctgt
     1981 tcgtcgcgat ccgcggccgc tggggccggg agctgccatt cctggtcatc gccgcggtga
     2041 tcggcacctt cgccggcaac ttcctcgccg cgctgctcga ggtggacgtt ctggtcctcg
     2101 gcgagttcca cctggtcggc gccgtcatcg gcgcccagct cgcgttgatc acgaccgcgg
     2161 cggtcgccac attgctcgcg ggaacccgcg gatccacggg ccgacgcgac ggggcgggtt
     2221 cgggccggta gaatcg
//
LOCUS       DASYGU010000110         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_585128, whole genome shotgun sequence.
ACCESSION   DASYGU010000110 DASYGU010000000
VERSION     DASYGU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585128"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGO32_02950"
     CDS             <1..658
                     /locus_tag="VGO32_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7199742.1"
                     /translation="YPDPTNFLDYHFNNDANLQFGTIDPSVTEPLTTGATTLDTEVRA
                     AAYAEANTAIKDTVPMVPIVHGASATAWLANVEGAHSSPLGNESMAHVTPADNQMVWM
                     QNAEPIALYCADETDGETLRLCEQVTEALYSYEVGGTTPIPALAEECVGNEDATEFTC
                     TLRQGVTFHNGAAFDAGDVVNSYAVQWDAAHPLHIGRTGSFEYWAGLFGAFLNPPPAE
                     "
     gene            776..1852
                     /locus_tag="VGO32_02955"
     CDS             776..1852
                     /locus_tag="VGO32_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199743.1"
                     /translation="MLQFVLRRMLLTVPVLIGVIFVVFAIARILPGDPCFVALGQRAT
                     PEACAAFDARYGLNEPIFVQFGIFVSDLFRGDLGESIAYGRPISLILIERLPVTIELT
                     LFALTIATFLGIALGVASAARRNSRTDVGTMMFANFGVSIPVFVLGLILIFFFAVVLK
                     DTPFALPPSGRLSAGVIIIPLAEAWGMQDLDGPLRVVIDFLGNMYVFSTLVTGQWAAF
                     GDAVVHLILPAIALATIPLALIARMTRSSLLEVLGQDYIRTARAKGVLERRVVLRHGL
                     RNAILPVVTVIGLTLGALLSGAVLTETIFGLTGIGKTITDSILGRDYPVIQAVTLITA
                     VIYLAVNLVVDVSYAFLDPRIRLE"
     gene            1849..2841
                     /locus_tag="VGO32_02960"
     CDS             1849..2841
                     /locus_tag="VGO32_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199744.1"
                     /translation="MKAPSTVPVGLEGLDEQAVAAMAIGEAAALTARPGNLWRDTAKG
                     ILRQRSAQVGLLLLTILITTAVLAPVIAPYDPEVAVGIEEGLSQADGEFAPCVHLFGC
                     PEEQPQHILGLDRNGRDVFSRVVWGSRVSLYVGVLTVGFAIVIGSVIGATAGFVGGGL
                     DNVLMRIMDILLVFPALVLAIAIVTVLGAGLINAQIAIGLVAIPIYARVMRASVLSVR
                     ERDFITASLALGESRRGILFRRIFPNSMTPLIVAGTLGVAGAILEVAALSFLGLGAQP
                     PLAEWGSMIALDRNQLFNAPWLILAPGIALTISVLGFNLFGDGLRDALDPRLNR"
     gene            2838..3917
                     /locus_tag="VGO32_02965"
     CDS             2838..3917
                     /locus_tag="VGO32_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199745.1"
                     /translation="MNVAEVTEIEEIPRRVGSAGQRLLDVRGLQTSFHTRDGVVRAVT
                     GVDFHVNRGEILGLVGESGCGKTVSALSILGLIAPPGRIERGEIMFDGVDLLTLEPEK
                     LRGLRGARISMIFQQPQSSLNPVYDVGRQIAEVLEIHRGMARKTARARAAELLRMVGI
                     PDPERRLESFPHALSGGMAQRVMIAMALACEPELLIADEPTTALDVTIQAQILDLMRA
                     LQADTGTAIILITHDLGVVAEMCDRVAVMYAGEIVEQTDTTTLFNDPKHPYTRGLIGS
                     VPTLGVLKDELTTIPGSVPNLVDLPAACRFAPRCLARVNQDVPYAEIHHPELRAVGDS
                     AVGHDVRCWIYHDLDGQPIAGAAVR"
     gene            3920..5062
                     /locus_tag="VGO32_02970"
     CDS             3920..5062
                     /locus_tag="VGO32_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7199746.1"
                     /translation="MATPNPNGALVQVENLTEYFPLRGGILQRKVGDVKAVDDVSFEI
                     RRGETLGLVGESGCGKTTVGRTLLRLIEPTAGKIIFDGNDITHVKGNDLKRYRRRMQI
                     VFQDPYASLDPRSPIATAIGEGLRIHGIGSAGERADKVSRMMDLVGLQPYHARRYPHE
                     FSGGQRQRIGIARALVLEPDLLVCDEPVSALDVSIQAQVLNLLRDLQRRLGLTYLFIA
                     HNMGVVEHISDRVAVMYLGALAEVADAKEMYRLPLHPYTIALLSAVPVAKPAERSARR
                     RIILRGDVPSPVNPPSGCRFHTRCWLRTQLGNPERCAVDRPVLRPLGTDHQVACHYAG
                     ELLEPDARQRRIAAAAAGSSGLASAEATDEAAPPTPPPVATDPVLT"
     gene            5073..5951
                     /locus_tag="VGO32_02975"
     CDS             5073..5951
                     /locus_tag="VGO32_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV7199747.1"
                     /translation="MEVRVALAQVAPRLGDVEANLDLAADRIREAAAEGAHLVVFPEL
                     SLTGYLLADLVPEVAMPITDPRLAEVSRQAPEALVALGFVEETNDHRYTNSAVLLRNG
                     EVIGHHQKVYLPTYGMFDEGRFTRTGDRIRTTAVGEPLGRIGLSVCEDFWHPSVAMIQ
                     AQDGASLLVNIAAGPARAPGSSAGLAAIAGWHKMQETYALLGTVAVAFCNRVGNEEGL
                     TFWGGSRLLGPNGAVLAEAPLYEEALVIGTLDTDDLRMQRYQLPILADERLELTRREL
                     DRIIAERAGLDPSEDA"
BASE COUNT          911 a         2202 c         2022 g          991 t
ORIGIN      
        1 ctaccccgat ccgaccaact tcctggacta ccacttcaac aacgatgcca acctgcagtt
       61 cggcaccatc gatccgtcgg tgaccgagcc cctgaccacg ggagcgacca cgctcgacac
      121 cgaggtccgg gcagcggcat atgctgaagc caataccgcg atcaaggaca ccgtgccgat
      181 ggttcccatc gttcacggcg cgtcggcgac cgcatggctg gcgaacgtcg agggggctca
      241 ctcctcgccg ctcggcaacg agtccatggc ccacgtgaca ccggctgaca accagatggt
      301 ctggatgcag aacgccgaac cgatcgccct ctactgtgcg gacgagacgg acggcgagac
      361 gctccgcctc tgcgagcagg tcaccgaggc cctgtactcc tacgaagtgg gaggcacgac
      421 cccgatcccg gctctggccg aggagtgcgt gggcaacgag gatgccaccg agttcacctg
      481 caccctgcgg cagggagtga cgttccacaa tggcgccgcc tttgacgccg gcgatgtcgt
      541 caactcctac gccgtgcagt gggatgctgc gcatccgctc cacatcggac gaacgggttc
      601 gttcgagtac tgggcgggcc tgttcggggc cttcctgaac ccgcctccgg ccgagtagcc
      661 acccgatacc gatgagcggg ctccggcccg ctcacggaga ccagcacgga cgccgccttc
      721 gggcggcgtc cgtcgctcta cccccatagg cgacagcgca ctacactcgc gcccaatgct
      781 ccagttcgtc ctgcgccgga tgctgctgac cgtcccggtt ctgatcggcg tcatcttcgt
      841 ggtgttcgcc atcgcccgga tcctgcccgg cgacccatgc ttcgtggccc tcggccagcg
      901 ggcgacgccg gaagcgtgcg cagcgttcga cgcgcggtac ggactgaacg agccgatctt
      961 cgtccagttc gggatcttcg tcagcgacct gttccggggt gatctcggcg aatcgatcgc
     1021 ctatggccgg cccatctcgc tgatcctcat cgagcggctg cccgtcacca tcgagctcac
     1081 gctcttcgcc ctgaccatcg ccacgttcct gggcatcgcc ctcggtgtgg cctcggccgc
     1141 gcggcgcaac tcgcgcacgg acgtcgggac catgatgttc gcgaacttcg gggtgtcgat
     1201 cccggtcttc gtgctgggcc tgatcctgat cttcttcttt gccgtggtcc tgaaggacac
     1261 gcccttcgcg ctgcctccca gcggccggct gtcggcgggg gtgatcatca tcccgctggc
     1321 cgaggcatgg ggcatgcagg acctggacgg tccgctgcgg gtggtgatcg acttcctcgg
     1381 caacatgtac gtgttcagca ccctggtcac cggccagtgg gcggcgttcg gcgacgcggt
     1441 cgtccacctc atcctgccgg ccatcgccct ggccaccatc cccctcgccc tcatcgcccg
     1501 aatgacccgc tccagcctgc tcgaggtcct cggccaggac tacatccgga ccgcgcgggc
     1561 gaagggggtc ctcgaacggc gggtggtcct gcgccacggc ctgcgcaacg cgatcctgcc
     1621 ggtggtcact gtcatcggcc tcaccctggg tgcgctgctg agcggcgcgg tgctgaccga
     1681 gacgatcttc ggcctgaccg gcatcggcaa gacgatcacc gactcgatcc tcggtcgtga
     1741 ctacccggtc atccaggcag tcaccctgat cacggccgtc atctacctgg ccgtgaacct
     1801 ggtggtcgac gtctcgtacg ccttcctcga cccacggatc cgcctcgaat gaaagcgccg
     1861 tcgacggtcc cggtcggcct cgaggggctc gacgagcagg ccgttgccgc catggccatc
     1921 ggcgaagccg cggcgctgac ggcgcggccg ggcaacctgt ggcgcgatac ggcgaagggc
     1981 atcctccgcc agcggtcggc gcaggtcggg ctcctgctgc tgacgatcct gatcacgacc
     2041 gcggtcctgg ccccggtcat tgccccgtac gatcccgagg tcgcggtagg catcgaggag
     2101 ggcctgtccc aggccgacgg cgagttcgcc ccgtgcgtcc acctgttcgg ctgtcccgag
     2161 gagcaaccgc agcacatcct gggcctcgac cgcaacggcc gcgacgtctt cagccgggtc
     2221 gtctggggat cccgcgtctc actgtacgtg ggggttctga ccgtcggctt cgcgatcgtc
     2281 atcggctcgg tcatcggcgc caccgcgggg ttcgtcggtg gcggcctcga caacgtgctc
     2341 atgcggatca tggacatcct cctggtcttc ccagccctgg tcctggccat cgcgatcgtg
     2401 accgtgcttg gcgcgggatt gatcaacgcc cagatcgcga ttggcctggt ggccatcccg
     2461 atctatgcgc gtgtcatgcg cgcatcggtc ctgtcggttc gcgagcgtga cttcatcacc
     2521 gcgtccctgg ccctgggcga gtcccggcgc gggatcctgt tccgccgcat cttcccgaac
     2581 tcgatgactc ccctcatcgt cgccggcacg ctgggcgtgg ccggcgccat cctcgaggtg
     2641 gccgccctct cgttccttgg cctcggtgcc cagccgccac tcgccgagtg gggatcgatg
     2701 atcgccctcg accgcaacca gctgttcaac gcgccgtggt tgatcctggc cccgggcatc
     2761 gccctgacca tcagcgtgct cggcttcaac ctgttcggcg acggcctccg cgacgcactc
     2821 gacccccgcc tcaatcgatg aacgtggccg aggtcacgga gatcgaggag atcccgcgcc
     2881 gggtcggcag cgccggccag cggctgctcg acgtgcgcgg cctccagacc tcgttccata
     2941 cccgcgatgg ggtggtccgg gcggtgacgg gcgttgactt ccacgtcaac cggggcgaga
     3001 tcctgggcct ggtcggggag tcaggctgcg ggaagacggt cagcgcgctg tccatcctgg
     3061 gtctcattgc gccgcccggt cggatcgaac ggggcgagat catgttcgac ggggtggacc
     3121 tgctgaccct cgagccggag aagctccgtg gcctccgcgg agcgcggatc agcatgatct
     3181 tccagcagcc gcagagcagc ctgaacccgg tctacgacgt gggccgtcag atcgccgaag
     3241 tgctcgagat ccatcgtggc atggccagga agaccgcccg agcgcgcgcc gccgagctgc
     3301 tccggatggt gggcatcccc gatcccgagc gaagactgga gtccttcccc cacgccctgt
     3361 ccggtggcat ggcccagcgg gtgatgatcg cgatggcgct ggcctgtgaa cccgagctcc
     3421 tgatcgcgga cgaacccacc accgccctgg acgtcacgat ccaggcccag atcctggacc
     3481 tcatgcgcgc gctccaggcc gacaccggga ccgcgatcat cctcatcacc cacgaccttg
     3541 gcgtggtggc cgagatgtgc gaccgggtgg cggtcatgta cgccggcgag atcgtcgagc
     3601 aaacggacac gaccaccctg ttcaacgatc cgaagcaccc gtacacccgc ggcctgatcg
     3661 gctccgtgcc gaccctcggg gtgctcaagg acgagctgac caccatcccg ggctcggtgc
     3721 cgaacctcgt ggacctgccg gccgcgtgcc gattcgcccc gcgctgcctg gcccgcgtga
     3781 accaggacgt cccatatgcc gagatccacc accccgagct ccgcgcagtc ggggacagcg
     3841 cggtgggaca cgacgtccgg tgctggatct accacgacct ggacgggcaa cccatcgcgg
     3901 gagcagcggt gcggtgacca tggccacgcc gaatccgaac ggtgccctgg tccaggtcga
     3961 gaacctgacc gagtacttcc cgctccgcgg cggcatcctc cagcgcaagg tcggtgacgt
     4021 gaaggcggtc gatgacgtga gcttcgagat ccgccgcggg gagacgctgg gcctggtggg
     4081 ggagtcgggc tgtggcaaga cgacggtcgg ccggaccctc ctccgcctga tcgagcccac
     4141 cgccgggaag atcatcttcg acgggaatga catcacccac gtcaagggca acgacctgaa
     4201 gcgctatcgg cgtcggatgc agatcgtgtt ccaggaccca tacgcgtcgc tcgacccacg
     4261 ctcgccgatc gccaccgcca tcggcgaagg actgcgcatc cacggcatcg ggtcggccgg
     4321 ggagcgggcg gacaaggtgt cccggatgat ggatctcgtc ggcctccagc cgtaccacgc
     4381 ccggcgctac ccgcacgagt tctcgggcgg ccagcggcag cggatcggga tcgcgcgggc
     4441 gctcgttctg gagcccgacc tcctcgtctg cgacgagccg gtcagcgcgc tcgacgtcag
     4501 catccaggcc caggtcctga acctgctccg cgacctccag cgtcggctgg ggctgaccta
     4561 cctgttcatc gcccacaaca tgggcgtggt cgagcacatc agcgaccggg tggcggtcat
     4621 gtacctcggc gccctggccg aggtcgccga cgcgaaggag atgtaccgcc tcccgctcca
     4681 cccgtacacc atcgccctgc tgtcggcggt gccggtggcg aagcccgccg aacggtcggc
     4741 ccgacggcga atcattctgc gcggcgacgt cccgtcgccg gtcaacccgc catcgggctg
     4801 ccgcttccac acccgctgct ggcttcggac ccagctcggc aatcccgagc ggtgcgcggt
     4861 cgaccggccc gtgctgcggc ccctcggtac cgaccaccag gtcgcgtgcc attacgcggg
     4921 ggaattgctc gagcccgatg cccgccagcg ccgcatcgcg gcggcggcgg ccggcagctc
     4981 cggcctggca tcggccgagg ccaccgacga ggccgctccg cccaccccgc caccggtcgc
     5041 gacggacccg gtcctcacat agacttcgcg acgtggaggt tcgcgtcgcg ctcgcccagg
     5101 tcgcgcctcg cctgggggac gtcgaggcca acctcgatct ggccgccgac cggattcgtg
     5161 aagcggccgc cgagggcgcc cacctggtcg tctttcccga gctctcgctg accggctatc
     5221 tcctcgccga cctcgttccc gaggtagcca tgcctatcac cgacccccga ctggccgagg
     5281 tgtcacgcca ggcgccggag gcgctggtcg cgctcggttt cgtggaggag accaacgacc
     5341 atcggtacac gaacagtgcc gtgctcctgc gtaacggcga ggtcatcggg caccaccaga
     5401 aggtctacct cccgacctac gggatgttcg acgaaggccg attcacccgg actggcgacc
     5461 ggattcgaac gacggcggtg ggggagcccc tgggacggat cggcctgagc gtgtgcgagg
     5521 acttctggca cccctcggtg gcgatgatcc aggcccagga cggggcgtcg ctgctcgtga
     5581 acatcgcggc cggtccggcc cgggcgccgg gcagcagcgc cgggttggcc gctatcgccg
     5641 gctggcacaa gatgcaggag acgtacgcgc tgctcgggac ggtggccgtc gcgttctgca
     5701 accgggtcgg caacgaggag gggctcacgt tctggggtgg ctcgcggctg ctcgggccca
     5761 acggagcggt actcgccgag gcgccgctgt acgaggaggc gctcgtgatc ggcacgctcg
     5821 acaccgacga cctgcgcatg cagcgttacc agctcccgat cctggccgat gagcggctcg
     5881 agctcacccg ccgcgagctg gaccggatca tcgccgagcg tgccgggctg gacccgtccg
     5941 aggacgcgtg accgcctccg ccagtcccga ggcggcgccg ctgccggaga tcgacgcggc
     6001 gcaggtgatc gaggtcatcg tgggctttgt tcgcagccag ctggagcaga ccggcttcga
     6061 gcggctggtc gtgggcctgt cgggcggggt ggacagtgcc acggtcgcgt acctcgccgc
     6121 ccgggc
//
LOCUS       DASYGU010000111         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1048253, whole genome shotgun sequence.
ACCESSION   DASYGU010000111 DASYGU010000000
VERSION     DASYGU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048253"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..975)
                     /locus_tag="VGO32_02980"
     CDS             complement(70..975)
                     /locus_tag="VGO32_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199748.1"
                     /translation="MSVQILGDAPVLDGSAVGQEWVMPAALTYTDEAYQLFGVTFDQE
                     NSVDPRGFYATSPDGLDWTVADGDPLAGISLNLSPPGPVPGSVLREDDGSWVMFFWGV
                     RAPLERGADLFRATAPAPTGPWTADPEPVLEGTIGAWDGAGLDFPSVVHTPDGYLMVY
                     AGSNLGAPNEGQFGVATSIDGAEWTKEPDPVIEPGFCGEFDSRSMAIPRLREVDDGYL
                     LFYNGLDPDLSTAEVGVASSLDGLTWMCASSQPVITGDDIPGSQGIHVIAVAAEAQGP
                     EFLVESLGDGSSSLWLGDVSMTGLP"
     gene            1219..1797
                     /locus_tag="VGO32_02985"
     CDS             1219..1797
                     /locus_tag="VGO32_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV7199749.1"
                     /translation="MSSWTHRLEVDSVVGEKVVAVDGRLGRAAAFTRLADDHLDAAYR
                     LAHAILGNRAEAEDATHDALVQAWRRWGQLHDHTRFEHWFDRILVNTCRSRLRQTNRR
                     LTRDLSPELAASGRDPTGQIDDRDQMRAALAQLSPDHRVVVALRFYRDLPISEIARRI
                     GVPEGTVNSRLHYALRHLRETLGDLEYPGADR"
     gene            1794..>2656
                     /locus_tag="VGO32_02990"
     CDS             1794..>2656
                     /locus_tag="VGO32_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199750.1"
                     /translation="MTNDQLEKRLHSWYLAEVRDDEAAPITLRGGLAEITTVPPAGLF
                     RDRRTFVLLAAAAVLAVAAIGSAVAIGSGFFRTPAPETDLLVPIFEPCEPALPEGVLL
                     TFDPAGRQADGPPSTVPRYVLYESGQTLRYEWSTATEWDAWRQRRMAPEGVRLLLDSV
                     QGVNQPSCEPLYLPEYASHAYAVTARSADDAVRQLYLGSGLFEVGFGTEAAHAAAADL
                     VTRFADPDLKLPPADWVDTDWQPYAPERYSVVAYFPTGMGDEPGSEAWDVTLPDGSTL
                     KTFGETTPGTEA"
BASE COUNT          428 a          922 c          891 g          415 t
ORIGIN      
        1 gtaccgaatg acctggccgg cgttaagggc ctgcctccgg gccggccctt cgaggtatcc
       61 cggtgttagc tatgggaggc cggtcatcga gacgtcgccg agccacagcg aagacgaccc
      121 gtctccgagc gactcgacca ggaactccgg accctgggcc tcggccgcga ccgcgatgac
      181 gtggattccc tggctgcccg ggatgtcgtc ccccgtgatc accggctgcg agctggcgca
      241 catccaggtc agcccatcga ggctcgaggc cacgccgact tcggcggtgg acaggtccgg
      301 gtccaggccg ttgtagaaga ggaggtagcc gtcgtccacc tcgcgcaggc gcggtattgc
      361 catcgagcgg ctgtcgaact cgccgcagaa accaggttcg atgaccggat ccggctcctt
      421 ggtccattcc gcgccatcga tcgaggtcgc gaccccgaac tgtccttcgt tcggcgcgcc
      481 gaggttgctg ccggcgtaca ccatcaggta gccgtcgggc gtatggacga ctgacgggaa
      541 gtcgaggccg gctccgtccc acgccccgat cgtgccttcc aggacgggct ccggatcggc
      601 ggtccacggt ccggtcgggg ccggcgccgt ggcccggaag agatctgccc cacgctccaa
      661 aggagcgcgg acgccccaga agaacatgac ccagctgccg tcgtcctcgc gcagcacgct
      721 gcccggtacc gggccaggcg ggctcaggtt gagggaaatg ccggccaaag ggtcgccatc
      781 ggcgacggtc cagtcgaggc cgtcaggcga ggtcgcgtag aagccccgcg ggtcgacgct
      841 gttctcctgg tcgaaggtga cgccgaacag ttggtaggcc tcatcggtgt aggtcagggc
      901 ggcgggcatc acccattcct gacccacggc gctgccgtcc agtaccggcg catcccccag
      961 gatctggacc gacactcccg ggccgagcga gggctcagtc gtcggagccg atggagccac
     1021 caatgccggt ggaccggcag atccgtcagg cgcgaccgac ggtgtcaggg tgcacgcggc
     1081 aaccgcgacc agggtcgccg ccgacggaag ggatcgcaag agtcgcattg ccatgactag
     1141 tcgtggggac cgttggttgg ttcacctgcc accaggcgct gagaatggag actgaagatt
     1201 tctcaccggc cgggcgtcat gtcatcgtgg acgcatcggc tggaggtgga tagcgtggtc
     1261 ggcgagaagg tcgtcgcggt cgacggacgc ctggggcggg ccgccgcctt cacccgcctg
     1321 gccgacgacc acctcgacgc cgcctaccgc ctggcgcatg ccattctcgg caatcgagct
     1381 gaggccgagg acgccaccca cgacgccctc gtccaggcct ggcgccgctg gggtcagttg
     1441 cacgatcaca cgcggttcga gcactggttc gatcggatcc tcgtcaacac ctgccgcagc
     1501 cggctgcggc agacgaaccg acgactgacg cgggacctct ctccagagct cgccgcttcc
     1561 ggacgtgatc ccacgggcca aatcgacgac cgcgaccaga tgcgcgccgc gttggcgcag
     1621 ctctcgcccg accatcgcgt ggtggtcgct ctccggttct accgcgatct ccccatctcc
     1681 gagatcgcga ggcgaatcgg ggtgcccgag gggaccgtca actcccgact ccactacgcg
     1741 ctgcgccacc tgcgcgagac gctcggtgat ctcgaatacc caggagcaga ccgatgacca
     1801 acgaccaact cgagaagcgc ctccactcct ggtacctcgc cgaggttcgc gacgacgagg
     1861 cggctccgat caccctgcgc ggtggcctgg ccgagatcac caccgtcccg cctgctggct
     1921 tgttccggga ccgacgcacg ttcgttctgc tggcggccgc ggcggtgctg gccgtcgccg
     1981 cgatcgggag cgccgtggcc atcggctcgg gtttcttccg cacaccggcg cccgaaaccg
     2041 acctgctcgt cccgattttc gaaccgtgcg agccggccct tcccgagggc gtcctgctga
     2101 ccttcgaccc ggcgggtcgg caggcggatg gaccgccatc gacggtcccg cgctacgtcc
     2161 tgtacgagag tggccagacc ctgagatacg aatggtccac ggccacggag tgggatgcat
     2221 ggcggcagcg acggatggcg cccgaggggg tacgactcct gctcgactcg gtccagggcg
     2281 tgaaccagcc gagctgcgag ccgctgtatc tcccggaata cgcttcgcat gcctacgcgg
     2341 tgactgctcg gtccgcagac gacgcagtcc ggcagctcta ccttggttcc ggactattcg
     2401 aggtcggatt cggcacggag gcggcgcatg ccgctgccgc cgatctcgtg acgcggttcg
     2461 ccgatccgga cttgaagctc ccaccggcgg actgggttga taccgattgg cagccctatg
     2521 cgccggagcg ctactccgtc gtggcctact tccccaccgg gatgggcgac gaaccgggct
     2581 cggaagcttg ggacgtcacg ctcccggacg gatccacgct caagacgttc ggcgagacga
     2641 cgcccggaac cgaggc
//
LOCUS       DASYGU010000112         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1150974, whole genome shotgun sequence.
ACCESSION   DASYGU010000112 DASYGU010000000
VERSION     DASYGU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150974"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..545
                     /locus_tag="VGO32_02995"
     CDS             246..545
                     /locus_tag="VGO32_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7199751.1"
                     /translation="MAIATPPAEAQRDRSAAVARALSDPKRLCVLENLADGGERSVGD
                     LSRNVGCQVPNMSQHLAVLRSAGIVASRRQGSTVLYRIADPSVLELVGALQRVAR"
     gene            583..924
                     /gene="trxA"
                     /locus_tag="VGO32_03000"
     CDS             583..924
                     /gene="trxA"
                     /locus_tag="VGO32_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV7199752.1"
                     /translation="MSSNVPEVTDATFEQLVTQAEKPVIVDFWAAWCGPCRLVAPEID
                     KLAEKYAGQVDVYKLDVDANPHTAAHFGIMSIPTVAYFQKDQPPRAAVGFRPMAALET
                     AFGLDTLAASA"
     gene            977..3010
                     /locus_tag="VGO32_03005"
     CDS             977..3010
                     /locus_tag="VGO32_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199753.1"
                     /translation="MFRRLRPVALVALALAVVGLVLPSAAPVALAGGGLVTVMDATYD
                     VMPEEARVHVTVDAESTSFERDAPEGPVYYAGLSMSIPAGSSNIAATAAGEPLPVTIT
                     PGTDETRLDIGFSEQVLLGDSYAYQLMFDMVDLGGASNRDFRIGHSVVAFPVWAFGSP
                     DADGSSVAVLLPPTFTPAVYGGPLVETTADDGSIHLTADTSNLPSWFAYVTAERPGIF
                     TATPFSVALGPDDATGQVHAWDDDPEWGARTVDLMLQGLPALYQLIGLDWPVVGDLRV
                     EESANRLGDYAGIYNRLTEKINVRYDADATVTLHEAAHVWFNNDLFRDRWIGEAWAEF
                     YAVQSADVIGVGGETFELTDDLLAAQIPLNDWEGIGAENRDVEAFAYAASFHLAELIF
                     ERTDIDGLRAVWRAAEDGEMAYQPIHGTAAPEQGVAFSSEDWQRLLDLLEERTAATYD
                     DLWTTWVVDPTENIMVGERAEARELYQSVVAAAGTWELPSTIRLNMGSWQFDAASAQL
                     DTADRVLDLAANLDLLADDLGLEPPDQLRAAFEGSAGLEAALTEANKELFALELIADA
                     THGLAAEQDPIEQIGLLFSEPTVALDAAETAWEAGDHGEATDQAEAVLTTLAEADDRG
                     RERVAVGGGILLVTLGGTALLVRRRRRDRNDDGAAIETPSDGPTDFPTDEPAD"
     gene            complement(3023..3394)
                     /locus_tag="VGO32_03010"
     CDS             complement(3023..3394)
                     /locus_tag="VGO32_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7199754.1"
                     /translation="MSSRVVHFELPADDPDRCAKFYADAFGWKVEKWGGPTDYWLVTT
                     GEATETGIDGGIGRRQGPDDQTTNTISVPDLDAAVAAVEAAGGTITRPKAAVPGVGWL
                     AYATDTEGTPFGIMQSDESAA"
     gene            3467..4783
                     /locus_tag="VGO32_03015"
     CDS             3467..4783
                     /locus_tag="VGO32_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199755.1"
                     /translation="MNRSIRAVLVGNFTLRFSTGLTGTLLLLYLADLPDYGGVVVSAT
                     TAGLMGALYFVAELILSPFFGVLADRFGTHRLMQPGPIMGAVAVIVTALTAIAGTAIT
                     ANVPVLAGTRFLEGAAAAASVPAALGYIAIATSRDQALRGRVIGRFEVATLGGVGIGS
                     VVAGFLYSALGPTAFYVNAAIYGITFLVLRYGVDDLPEHIPPAPGEETVPASLFHLGR
                     YRDVLRSRPIWFLAPTWIALNALLGSWGTQAIFQLVQEPSPEFSDQLLMQGIEPWQAG
                     LVLGVGLLVLFSGLLYWGTRFTRLRRTTIIGLGLGGGLLFVGALLALNHSTGQAAWFI
                     VATLVAAGIGVFVLAGATPAAVGFLSDISETYPGRRGVIMGLYSVFLALGQIIGAVVA
                     GVAAEWRGIDGLLVASAGLLIVALIPLGQLRAQEHHMPGSELAPAA"
BASE COUNT          716 a         1819 c         1640 g          830 t
ORIGIN      
        1 gcgtaggtca cggcggggtc gggcgcgaag tcgaagctcg tcccgcacgc gctgcacagc
       61 agctcgtccg agttgtagtc gacctcgaac gcgacctcgt cggcgcacca ggggcagatc
      121 aagtgaacag tcatggccgc atcgtgagcg tggcttgtga catctagcgc gtcgcaattc
      181 acgagactca cggattcccc gatgacttgc gcatttgcgc aactaccgat atcatgaacg
      241 gtgtcatggc cattgccact ccccctgctg aggcacagcg cgaccgttcg gccgctgtcg
      301 cccgggccct ctcggacccg aagcggctct gtgtcctcga gaatctcgcc gacggcggcg
      361 agcgatccgt cggtgacctg tcccgcaacg tcggctgcca ggtcccgaac atgagccaac
      421 acctggccgt cctgcggtcc gccggcatcg tcgccagccg ccgccagggc agcaccgtcc
      481 tgtatcgcat cgccgatccc tccgtcctgg agctggtcgg cgcgctgcag cgggtcgccc
      541 gctgacccgc attccgagtt cacgaaagga acccacatcc ccatgtcgtc caacgtcccc
      601 gaggtcaccg acgccacgtt cgagcagctc gtgacccagg ccgaaaagcc ggtcatcgtc
      661 gatttctggg ctgcctggtg cggcccgtgt cgtctggtcg ccccggagat cgacaagctg
      721 gcggagaagt acgccggcca ggtcgacgtc tacaagctcg atgtcgatgc caatccgcat
      781 accgcggccc atttcgggat catgagcatc ccgaccgtcg cctacttcca gaaggatcag
      841 cccccgcgcg ccgcggtcgg attccgaccg atggccgccc tggagaccgc gttcggcctc
      901 gacaccctcg ccgcctctgc ctgaccccgc cgagcacccg ttcgcgcccc gcctcggggc
      961 gcgctacggt gtagccgtgt tccgccggtt gcggccggtc gcgctcgtag ccctggcgct
     1021 tgccgtggtg ggattggtcc tgccctcggc cgctccggta gccctcgccg gcggcggact
     1081 ggtcacggtc atggatgcca cctatgacgt catgcctgaa gaggcccgcg tccacgtcac
     1141 ggtcgacgcc gagtcgacct cgttcgagcg cgacgcgccc gagggtccgg tctactacgc
     1201 cggcctgagc atgtcgatcc cggccggctc gagcaacatc gcagccaccg ccgccggcga
     1261 gccgctcccc gtgaccatca cgcccggcac cgacgagaca cgcctcgaca tcggcttctc
     1321 cgagcaggtc ctcctcggtg acagctacgc ctaccagctc atgttcgaca tggtcgacct
     1381 cggcggagcg tcgaatcgcg acttccggat cggccacagc gtagtggcct tcccggtgtg
     1441 ggcctttggc agccccgacg ccgatggcag cagcgtcgcc gttctcctgc ctcccacctt
     1501 cacgccggcg gtctacggtg ggccactggt cgagaccacc gctgacgatg gatcgattca
     1561 cctgaccgct gacacgtcga acctcccgag ctggttcgcg tacgtcaccg ccgagcgacc
     1621 cggcatcttc accgccactc cgttctcggt tgcgctgggc ccggacgacg cgaccggcca
     1681 ggtccacgcc tgggacgacg accccgagtg gggcgcccgg accgtcgacc tcatgctcca
     1741 gggccttccc gcgctgtacc agctcatcgg gctcgactgg ccggtggtcg gcgaccttcg
     1801 ggtcgaggag tcggcaaacc gcttgggcga ctacgcgggg atctacaacc ggctgaccga
     1861 gaagatcaac gtccgctacg acgccgatgc cacggtcacc ctgcatgaag ctgcccatgt
     1921 ctggttcaac aacgacctgt tccgcgaccg ctggatcggc gaggcctggg ccgagttcta
     1981 cgcggtccag tcggcggacg tgatcggcgt cggtggagag accttcgaac tcaccgatga
     2041 cctgctcgcg gcacaaatcc cgctcaacga ctgggagggc atcggggccg agaaccggga
     2101 cgtggaagcg ttcgcctacg ccgcgtcgtt ccacctggcg gagctgatct tcgagcggac
     2161 cgacatcgac ggcctgcggg ctgtctggcg ggccgccgag gacggggaga tggcgtacca
     2221 gccgatccac gggaccgccg ccccggagca aggcgtggcg ttcagctcgg aagactggca
     2281 gcgattgctg gacctgctcg aggagcgcac ggccgccacc tacgacgacc tgtggaccac
     2341 ctgggtcgtg gatccaacgg agaacatcat ggtcggcgaa cgggccgagg cccgcgagct
     2401 gtaccagtca gtcgtcgccg ccgcaggcac gtgggagctg ccgagcacga tccggctcaa
     2461 catgggcagc tggcagttcg acgcggcgag cgcccagctg gacacggccg atcgcgtgct
     2521 ggacctggcc gccaacctcg acctcctggc cgacgacctc ggcctcgagc cgcccgatca
     2581 gctcagggcc gcgttcgagg ggtccgcggg gctggaggcg gccctgaccg aggccaacaa
     2641 ggagctgttc gcgctcgagc tcatcgccga cgcgacccac ggcctcgcgg ccgagcagga
     2701 tccgatcgag cagatcgggc tcctgttctc cgagccgacg gtcgcgctcg acgcagcgga
     2761 aaccgcctgg gaagcgggtg accacggaga ggcgaccgat caggccgaag ccgtgctcac
     2821 caccctggcc gaagccgacg accgtggacg cgagcgggtg gccgtcggtg gcgggatcct
     2881 gctcgtgacc ctgggtggca ccgcgctcct ggttcgccgc cgccgccgcg accgaaatga
     2941 tgacggagcc gctatcgaga ccccatcgga cggcccgact gacttcccga cggacgagcc
     3001 cgccgactga ggaagtcgtg ctctaggcgg ccgactcgtc ggactgcatg atcccgaacg
     3061 gcgtgccctc ggtatcggtc gcgtaggcga gccagccgac gccgggcacc gctgccttgg
     3121 gtcgggtgat cgttccgccc gcggcctcca ccgccgcgac cgccgcgtcg agatccggca
     3181 cgctgatcgt gttcgtcgtt tggtcgtccg ggccctggcg acggccgatc ccgccgtcga
     3241 tccccgtctc ggtcgcctcg ccggtggtga ccagccagta gtcggtgggg ccgccccact
     3301 tctcgacctt ccagccgaag gcatcggcgt agaacttcgc gcagcggtcc ggatcgtcgg
     3361 ccggaagctc gaaatggacg actcgggaag acacggccac ctccggggtt tcgtgattgt
     3421 cgggacaatt gtcctacctc accctcccgt agactcgttc atcttcgtga accgctcgat
     3481 tcgcgccgtc ctcgttggca acttcacgct gcgcttttcg accgggctga ccggcacgct
     3541 cctcctgctc tacctcgccg atctgcccga ctacggcggg gtggtcgtat cggcgacgac
     3601 ggccggcctc atgggcgcgc tgtacttcgt ggccgagctg atcctctccc cgttttttgg
     3661 ggtgctggcc gaccggttcg gcacgcaccg actcatgcag cccggcccga tcatgggcgc
     3721 cgtcgccgtg atcgtgaccg ccctcaccgc gatcgcgggc acggcgatca ccgcgaacgt
     3781 gccggtcctc gccgggacac gcttcctcga gggcgcggcg gcagcggcca gcgtcccggc
     3841 agccctgggc tacatcgcca tcgccacgtc gcgcgaccag gctctccggg ggagggtcat
     3901 cggccgtttc gaggtggcca ccctgggcgg ggtgggcatt ggctcggtcg tcgcggggtt
     3961 cctgtattcc gccctgggcc ccaccgcctt ctacgtcaac gcggcgatct acggcatcac
     4021 cttcctcgtc ctgcgctacg gggtggatga cctgcccgag cacatccctc ccgcgccggg
     4081 tgaggaaacc gtccccgcct ccctgttcca cctcggacgc taccgggacg tgctccgcag
     4141 ccgcccgatc tggttcctgg cgccgacctg gatcgcgctc aacgccctgc ttggctcgtg
     4201 gggtacccag gcaatcttcc agctcgtcca ggaaccgtcg cccgagttct ccgaccagct
     4261 cctgatgcag ggcatcgagc cgtggcaggc cggcctggtg ctgggcgtgg ggctgctggt
     4321 gctgttctcc ggcctgctgt actggggcac ccgcttcacc cgcctgcggc ggacgacgat
     4381 catcggcctg ggattgggcg gcggcctcct gttcgtggga gcgctgctgg ccctcaacca
     4441 ctcgaccggg caggccgcgt ggttcatcgt cgcgaccctg gtggccgccg gcatcggcgt
     4501 gttcgtcctg gccggggcga cgccggcggc ggtcggcttc ctgtccgaca tcagcgagac
     4561 ctaccccggt cgacggggcg tgatcatggg cctgtactcg gtcttcctgg ccctgggcca
     4621 gatcatcggc gccgtcgtgg ccggcgtggc cgccgagtgg cgcgggatcg acggcctgct
     4681 ggtggccagc gccgggctgc tgatcgtggc cctcatcccg ctcggccagc tgcgggccca
     4741 ggagcatcac atgccgggct ccgagctggc gccagccgcg tagtgggcac cggctggctg
     4801 gcgcgcctcg gtatcctagg cggccaatgc gcctgagccg ccttttcttc acgaccctcc
     4861 gcgacgaccc gaccgatgcc gagatggtgt cgcacaagct cctcgtgcgc gcgggctacg
     4921 tccggcagct cggatccggc atctactccc tcctccctct gggcttcgcc gtcagccaac
     4981 gcgtggagca ggtcatccgc gagga
//
LOCUS       DASYGU010000113         5625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1151654, whole genome shotgun sequence.
ACCESSION   DASYGU010000113 DASYGU010000000
VERSION     DASYGU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151654"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /gene="rplW"
                     /locus_tag="VGO32_03020"
     CDS             <1..308
                     /gene="rplW"
                     /locus_tag="VGO32_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV7199756.1"
                     /translation="GVRMSARTIHDVLLRPVVSEKSVMQIERNNYTFAVRPDANKFQI
                     KAAVEAEFKVTVLGVRVLTVRPKQKSRGRRTKGMVSGWRKAIVTIADGDKIELFEAI"
     gene            310..1140
                     /gene="rplB"
                     /locus_tag="VGO32_03025"
     CDS             310..1140
                     /gene="rplB"
                     /locus_tag="VGO32_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV7199757.1"
                     /translation="MPLRSYRPNTPSQRHMTRSTFEEITTDQPHKPLTSNLNSKAGRN
                     SQGKLTVRHQGGGHKRLYRIIDWKRDKAGVPARIATIEYDPNRSARIALLHYADGAKR
                     YMLLPHGLSVGDTVASGPEVEARVGNALPLARIPLGTQIHNIELVAGRGGQIVRSAGT
                     AAQLLAKEGEMATIRLPSGEVRRVRAVCMATIGQVSNLDHENQKIGKAGRNRHLGRRP
                     SVRGVVMNPNDHPHGGGEGKSPIGMPPKTPWGKPAMGLRTRKNQSSDRLIVRRRYGRG
                     "
     gene            1150..1434
                     /gene="rpsS"
                     /locus_tag="VGO32_03030"
     CDS             1150..1434
                     /gene="rpsS"
                     /locus_tag="VGO32_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV7199758.1"
                     /translation="MSRSVKKGPFVDPRLLGRIDEMNKRKEQKVLKTWSRASVIFPAM
                     IGHTIAVHDGRRHVPVFVTENMVGHRLGEFAPTRTFRGHGRHTERSSALK"
     gene            1445..1786
                     /gene="rplV"
                     /locus_tag="VGO32_03035"
     CDS             1445..1786
                     /gene="rplV"
                     /locus_tag="VGO32_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV7199759.1"
                     /translation="MRVSATAKYVRISPRKVRLVTDMIVGRPVDEAAAILRFLPNAAA
                     RDVAKVLRSATANAENNFNLTADELHVESAIANEGPTLKRIRPRAQGRAFQILKRTSH
                     ITVSVADRGDA"
     gene            1786..2406
                     /gene="rpsC"
                     /locus_tag="VGO32_03040"
                     /pseudo
     CDS             1786..2406
                     /gene="rpsC"
                     /locus_tag="VGO32_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            2747..3160
                     /gene="rplP"
                     /locus_tag="VGO32_03045"
     CDS             2747..3160
                     /gene="rplP"
                     /locus_tag="VGO32_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV7199760.1"
                     /translation="MLLPKRVKHRKVQRGRRGGLAKGGTTVSFGEFGLVAEEVCWLTS
                     RQIEAARRAMTHHIKRGGRVWIRVFPDKPVTKKPAEVRMGSGKGAPDHWVAVVKPGRV
                     MFEMSGVPEPIAREAMRLASHKLPISTKFVVKEGA"
     gene            3163..3393
                     /gene="rpmC"
                     /locus_tag="VGO32_03050"
     CDS             3163..3393
                     /gene="rpmC"
                     /locus_tag="VGO32_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018021286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV7199761.1"
                     /translation="MDISEVRAMSDADLATALADAKQELWKIRFDLATRQEKNYSRLP
                     ATRQRIARILTVMTERQRAATTAAPAASQESA"
     gene            3393..3629
                     /gene="rpsQ"
                     /locus_tag="VGO32_03055"
                     /pseudo
     CDS             3393..3629
                     /gene="rpsQ"
                     /locus_tag="VGO32_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            3743..4111
                     /gene="rplN"
                     /locus_tag="VGO32_03060"
     CDS             3743..4111
                     /gene="rplN"
                     /locus_tag="VGO32_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV7199762.1"
                     /translation="MIRRQTRLRVADNTGARELMCITVVGRSTSETASVGDVIVASVK
                     QALPNSGVKKGEVVRAVIVRTTKGYGRPDGSHIRFDENAAVLITPQGNPRGTRIFGPV
                     ARELRERNYMKIISLAPEVL"
     gene            4111..4446
                     /gene="rplX"
                     /locus_tag="VGO32_03065"
     CDS             4111..4446
                     /gene="rplX"
                     /locus_tag="VGO32_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV7199763.1"
                     /translation="MAVRKGDTVMILAGKDRGKRGKVERVERTRRGLAAIIPGINMAK
                     RHQRSKGGRQQTAGIIDMPMPVQISNVMVVCPNCDKPTRIGHARLDDQHKTRVRVCKH
                     CAEQLEVGK"
     gene            4638..5165
                     /gene="rplE"
                     /locus_tag="VGO32_03070"
     CDS             4638..5165
                     /gene="rplE"
                     /locus_tag="VGO32_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV7199764.1"
                     /translation="MRYRDEAVPVLLREFRYANAMEVPRLDKVVVNIGLGEAIQNAKA
                     IDAAVGDLMIITGQKPIVTRATRSIAQFRLRTGMPVGAKVTLRGQRMYDFLERTMRLA
                     LPRIRDFRGVSPRSFDGRGNFSLGFREQLMYPEIDYDKIDRLRGFEISIVTTARTDEE
                     GRRLLALLGMPFAAS"
     gene            5181..5366
                     /locus_tag="VGO32_03075"
     CDS             5181..5366
                     /locus_tag="VGO32_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEV7199765.1"
                     /translation="MAKKSMIAKAKRTPKFPVRGYNRCTVCGRPRAYMRRFALCRICF
                     RERALEGMLPGVTKSSW"
     gene            5378..>5625
                     /gene="rpsH"
                     /locus_tag="VGO32_03080"
     CDS             5378..>5625
                     /gene="rpsH"
                     /locus_tag="VGO32_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV7199766.1"
                     /translation="MTDPIADMLTRVRNASLARHTEVVLPGSRVKTEIARILVEEGFI
                     ASFENRVEQGHDHLVLTLKYVEGKSPVVTGLKRISKPGL"
BASE COUNT         1029 a         1968 c         1898 g          730 t
ORIGIN      
        1 gaggtgttcg catgagcgcg cgcacgatcc acgacgtcct cctccgcccg gtggtcagcg
       61 agaagtcggt gatgcagatc gagcggaaca actacacctt cgccgtccgt cccgacgcga
      121 acaagttcca gatcaaggcc gcggtcgagg ccgagttcaa ggtcaccgtg ctcggcgttc
      181 gggtcctgac cgtgcgcccc aagcagaaga gccgcgggcg acggacgaag ggcatggtca
      241 gcggctggcg caaggcgatc gtgaccatcg ccgacggcga caagatcgaa ctgttcgagg
      301 cgatctgaca tgcccctgcg cagctatcgg cccaacaccc cgtcccagcg gcacatgacc
      361 cggtcgacct tcgaggagat cacgaccgac cagccgcaca agccgctgac ctcgaacctc
      421 aattcgaagg ccggtcggaa ctcccagggc aagctgaccg tccgccacca gggcggcggc
      481 cacaagcgcc tgtaccggat catcgactgg aagcgcgaca aggccggcgt gccggcgcgg
      541 atcgcgacca tcgagtacga ccccaaccgc tcggcccgga tcgcgctcct ccactacgcc
      601 gacggcgcga agcggtacat gctcctgccc cacgggctga gcgtgggcga cacggtcgcc
      661 tcgggcccgg aggtcgaagc ccgggtcggg aatgcgctcc cgctggcgcg gatcccgctc
      721 gggacccaga tccacaacat cgagctggtc gccggtcgcg gcggtcagat cgtacgcagc
      781 gccggcaccg ccgcccagct cctggccaag gagggcgaga tggcgaccat ccgcctcccg
      841 tccggcgaag tgcgccgtgt gcgcgccgtg tgcatggcga ccatcggcca ggtgagcaac
      901 ctcgaccatg agaaccagaa gatcggcaag gccggccgca accggcacct cggccgccgt
      961 ccgagcgtgc gcggcgtggt gatgaacccc aacgaccacc cgcacggtgg tggcgagggc
     1021 aagtcaccca ttggcatgcc gcccaagacc ccgtggggca agcccgccat gggcctgcgc
     1081 acccgcaaga accagtccag cgaccgattg atcgtccgcc gccggtacgg gcgcgggtaa
     1141 ggagacgcga tgagccgttc cgtcaagaaa ggtccgttcg tcgacccacg actgctgggt
     1201 cgtatcgacg agatgaacaa gcgcaaggag cagaaggtgc tgaagacctg gtcgcgggcc
     1261 tcggtcatct tcccggccat gatcggtcac acgatcgccg tccacgacgg tcgccgccac
     1321 gtcccggtgt tcgtgaccga gaacatggtc ggccaccgcc tgggcgagtt cgcgccgacc
     1381 cgcacgttcc gcggccacgg ccgccacacc gaacgctcct cggcattgaa gtaggaccag
     1441 accgatgcgc gtttccgcca ccgccaagta cgtccggatc agcccccgca aggtgcggct
     1501 ggtgaccgac atgatcgtcg gccgcccggt ggacgaggcg gcggccatcc tccgcttcct
     1561 gcccaacgcg gcggcccgcg acgtggccaa ggtgctgcgc agcgcgaccg ccaatgccga
     1621 gaacaacttc aacctgaccg ccgacgagct ccatgtcgag tcggcgatcg ccaacgaggg
     1681 cccgaccctg aaacgcattc gaccacgcgc ccagggccgc gccttccaga ttctgaagcg
     1741 caccagccat atcacggttt ccgtggctga tcgaggagac gcctgatggg ccacaaggtt
     1801 cacccctacg gattccggat cgggatcaac aagccgtggc tggcgaagtg gtacgccgat
     1861 cgtgactacg cgaccttgct ccaggaggac atgaagatcc gcgccctggt cgcgaagcag
     1921 ctgtccaacg cgagcgtcag ccaggtcgag atcgagcgcg gcatcaacca cgtgaccgtc
     1981 accgtccaca ccgccaagcc ggggatcgtc atcggcaagg gcggcgccaa cgtggagctc
     2041 ctgcgcaact cgatcggcaa gctgaccgac aagaaggtca agctcgagat caaggaggtc
     2101 ctccagcccg agctcgacgc cgtcctcgtg gcccagagca tcgcgggcca gctcgagcgc
     2161 cggattgcat tccgcaaggc cgtcaagcag ggcgtccagc gcagcatcaa ggctggggcc
     2221 aagggcgtga agatccaggt gtcgggtcgc ctcggcggca ccgagatgtc ccgccgggag
     2281 tgggacaagg agggacggat cccgcttggc acgctccgcg ccgacatcag ctacggcgtc
     2341 gtccacgccc ataccaccta cggtcggatc ggggtcaagg tctggatcta cagaggcgag
     2401 accctggccg atctgccggg ctccgcccgc accgagccgc gcgaggcgcg gaccgccgga
     2461 cgggcccgac cgaagtcgcg gagccgggcc gcggttgcgg cccccgagtc tgatggcgag
     2521 acttccgccg tcgccgtcga ggacgctccg acgccgcaga ccgaggcggt gaccgagggc
     2581 gagacagcca caccggccgc ccaggtcgag gacgtcgcca aggagcccgc ggtcgaaacg
     2641 gccgtcgagg tcgccgcgga gcccgagccg gcacccgccg atcccgagcc cgaggtcgct
     2701 cccgagcccg agccggccgg cgacgacgcg aaggagaagg actgacatgc tgctgccgaa
     2761 gcgcgtcaag caccgcaagg tgcagcgcgg tcgccggggt ggactggcca agggcggcac
     2821 gaccgtgtcg ttcggtgagt tcggcctcgt ggccgaagag gtgtgctggt tgaccagccg
     2881 ccagatcgag gctgcccgac gggcgatgac ccaccacatc aagcgtggcg ggcgggtctg
     2941 gatccgggtg ttccccgaca agccggtgac caagaagccg gccgaggtcc ggatgggatc
     3001 gggcaaggga gcgcccgacc actgggttgc ggtcgtgaag ccgggccggg tgatgttcga
     3061 gatgtccggc gtgccggagc cgatcgcccg cgaggcgatg cggctggcca gccacaagct
     3121 cccgatctcc accaagttcg tcgtgaagga aggcgcatga gcatggacat cagtgaagtt
     3181 cgcgccatga gcgacgccga cctcgcgact gccctggccg atgccaagca ggagctgtgg
     3241 aagattcggt tcgacctggc cacccgccag gagaagaact acagccggct gccggcgacc
     3301 cgccagcgga tcgcccggat cctgaccgtc atgaccgagc gccagcgcgc ggcgaccacc
     3361 gcggcaccgg ccgccagcca ggagtctgcc tgatggccgg ccagcgacgg accaaggtcg
     3421 ggcgcgtcgt gtctgacaag atggacaaga cggtcgtggt cagcgtcgag cgccttcagc
     3481 ggcacccgat ctacaagcgg gtcatccgcc gatcgaccaa gttcaaggcc catgaccagg
     3541 acaatgccgc ccaggttggt gacacggtcc ggatcgagga gagccggccg ctgtcgcgtg
     3601 acaagcgctg gcgcgtcgtc gagatcctgg gccatggccc ctcggagacc gcgacgggac
     3661 cgatggccga cgaggccatt ctgaatcccg agccggtggc cgatgcagac gccgagcctg
     3721 cggccgtgga gggagagtcc gcgtgatccg ccgccagacc cggctccggg tcgccgacaa
     3781 caccggcgcc cgtgagctca tgtgcatcac ggtggtgggc cgctccacga gcgagacggc
     3841 ctcggtcggc gacgtcatcg tggccagcgt caagcaggcc ctgcccaact cgggggtcaa
     3901 gaagggcgag gtcgtccgcg cggtcatcgt ccgcacgacg aagggctacg gccgccccga
     3961 cgggagccac atccgcttcg acgagaacgc cgccgtgctc atcacgcccc agggcaaccc
     4021 gcgcgggacg cgcatcttcg gacccgtcgc ccgcgagctc cgggagcgca actacatgaa
     4081 gatcatctca ctcgccccgg aggtgctgtg atggcagtcc ggaaaggcga caccgtgatg
     4141 atcctggccg gcaaggaccg cgggaagcgg ggcaaggtcg agcgcgtcga gcggacgcgc
     4201 cgtggcttgg ccgcgatcat ccccggcatc aacatggcca agcgccacca gcgctcgaaa
     4261 ggtgggcggc agcagacggc gggcatcatc gacatgccga tgcccgtcca gatcagcaac
     4321 gtgatggtgg tctgccccaa ctgcgacaag ccgacccgga tcggtcatgc ccggctggac
     4381 gaccagcaca agacccgggt ccgggtgtgc aagcactgcg ccgagcagct ggaggtgggc
     4441 aagtgaccga cgagccgacc aaggccgctc gcaagcccgc caccaagcct ggcgcatcgg
     4501 ccaagcccgc cgcgcgggcc aagcctgccg cgaagccgtc gacgagccgg gcgaaggccg
     4561 agccggccgc tccgaccgag gcctcggcgc cgtcgcgacg ccgcgccgcc ggccgccccg
     4621 agcccagcgg tctccggctg cgctaccggg acgaggcggt gcccgtcctg ctccgcgagt
     4681 tccgctatgc gaacgcgatg gaggtgccgc gcctggacaa ggtcgtcgtc aacatcggcc
     4741 tgggcgaggc catccagaac gccaaggcga tcgatgccgc ggtgggtgac ctgatgatca
     4801 tcaccggcca gaagccgatc gtgacccggg ccacacgctc catcgcccag ttccgcctgc
     4861 ggaccggcat gccggtgggt gccaaggtga cccttcgggg ccagcggatg tacgacttcc
     4921 tggagcgcac gatgcgcctc gcgctgcccc ggatccggga cttccgtgga gtctcgccgc
     4981 gctcgttcga cggccgcggc aacttcagcc tcggattccg cgagcagctg atgtaccccg
     5041 agatcgacta cgacaagatc gaccgcctcc gcggcttcga gatcagcatc gtcaccaccg
     5101 cccgcacgga cgaggaaggc cgccgcctcc tcgccctgct gggcatgccc ttcgccgcca
     5161 gctgaactga ggaccgaact gtggccaaga aatcgatgat cgccaaagcc aagcggaccc
     5221 cgaagttccc ggttcgcggg tacaaccgct gcaccgtgtg cggccggccg cgcgcataca
     5281 tgcgccggtt tgcgctgtgc cggatctgct tccgggagcg tgccctcgag gggatgctgc
     5341 ccggcgtgac gaagtcgagc tggtagtcgc catgaacatg accgatccca tcgccgacat
     5401 gctgacccgg gtgcggaacg ccagcctcgc gcggcatacc gaggtcgtgc tgcccggatc
     5461 ccgggtcaag accgagatcg cccgcatcct tgtcgaggag ggcttcatcg cctcgttcga
     5521 gaaccgggtc gagcagggtc acgaccacct ggtcctcacc ctgaagtacg tcgagggcaa
     5581 gtccccagtc gtcaccggcc tgaagcggat cagcaagccc ggact
//
LOCUS       DASYGU010000114         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_894797, whole genome shotgun sequence.
ACCESSION   DASYGU010000114 DASYGU010000000
VERSION     DASYGU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894797"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGO32_03085"
     CDS             <1..192
                     /locus_tag="VGO32_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199767.1"
                     /translation="VVESAEAAPEAPADGDDHDAEADAAADAPAAEAPAAEAPVSTDA
                     WPEKPDDDAPSAPKEDPTA"
     gene            192..1076
                     /gene="atpG"
                     /locus_tag="VGO32_03090"
     CDS             192..1076
                     /gene="atpG"
                     /locus_tag="VGO32_03090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEV7199768.1"
                     /translation="MASLRDIRRRIGSIRNIAQITRAMEMVAASRMKRAQESILAARP
                     FAEELDDVLARVAAAVDHTEEPLLARRPVRHVALVMITTDRGLAGALNSNAVRNALRW
                     VADRAGSQNGGEPVRVSAITVGRKGRDALRRAGIPIAAHFPQFGDRPVFADVQPLAKL
                     VTEDFLAETYDEVIVSYSQFISTLVQRPAIKSVLPVHEPTLTEGQRIRNDEYLFEPSA
                     EVLSRLLPYYVAVDIYRAVLENMASEQSARMIAMRNSTDNAHELIGELTLVYNKTRQA
                     TITREMIEIASGAEAQAS"
     gene            1106..>2436
                     /gene="atpD"
                     /locus_tag="VGO32_03095"
     CDS             1106..>2436
                     /gene="atpD"
                     /locus_tag="VGO32_03095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV7199769.1"
                     /translation="MASGKVIQITGPVVDVEFPAGELPEIYNALEIGELVVEVQQHLG
                     NNWVRAVAMSTTDGLARGVDAIDTGKAITVPVGEPTLGRVFDVLGHPIDGKGPVVSEH
                     MLPIHREAPAFEDMETEAAVFETGIKVIDLIAPFKRGGKVGVFGGAGVGKTVIIQELI
                     RNIAAEHGGYSVFAGVGERSREGNDLIGEMTESGVIDKTVMVFGQMNEPPGARQRVGL
                     TALTMAEYFRDVEGRDILLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGDL
                     QERITSTKKGSITSLQAIFVPADDYTDPAPATTFGHLDSTIRLERSIVELGIYPAVDP
                     LTSTSTVLDPRVVGEDHFQVAREVQQTLQRYRDLQDIIAILGMDELSEEDKVTVSRAR
                     RLQRFMSQPMFVAAQFTGRDGKYVERKETVRGFREILDGKHDQLPEQAFYMV"
BASE COUNT          425 a          841 c          808 g          362 t
ORIGIN      
        1 gtggtcgaat cggcagaggc cgcccccgag gcgcccgccg atggcgacga ccatgacgcc
       61 gaggccgatg ccgccgccga cgcaccggca gccgaggcac cggctgcgga agctccggta
      121 tcgaccgatg cgtggcccga gaagccggac gacgacgcgc cgagtgcccc caaggaggat
      181 ccgaccgcct gatggcatcc ctccgcgaca tccggcgccg catcggctcc atccggaata
      241 tcgcccagat cacccgggcg atggagatgg tggccgcctc gcgcatgaag cgtgcgcagg
      301 agtcgatcct cgccgcgcgt ccgtttgccg aggagctgga cgacgtgctg gcgcgggtcg
      361 ccgcggccgt ggaccatacc gaggagccgc tcctggcccg tcgccccgtg cgccacgtgg
      421 cgctggtgat gatcacgacc gaccgcggac tggcgggcgc gctgaactcg aacgcggtgc
      481 gaaacgcgct gcgctgggtc gcggaccgag ccgggtcgca gaacggcggg gagccggtcc
      541 gcgtatcggc catcacggtt ggtcgcaagg gccgcgatgc gctccgccgg gccgggatcc
      601 cgatcgccgc ccactttccg cagttcgggg atcggccggt cttcgcggac gtccagccgc
      661 tggccaagct cgtgaccgag gatttcctgg ccgagaccta cgacgaggtc atcgtcagct
      721 acagccagtt catcagcacc ctcgtccagc gaccggcgat caagagcgtc cttcccgtcc
      781 atgagccgac cctcaccgag ggccagcgga tccggaacga cgagtacctg ttcgagccat
      841 cggcggaggt gctgtcgcgc ctgctgccgt actacgtcgc ggtggacatc tatcgagccg
      901 tgctcgaaaa catggcctcc gagcagtcgg cccgcatgat tgcgatgcgg aactcgaccg
      961 acaacgccca cgagctcatc ggcgagctca ccctcgtcta caacaagacc cgccaggcca
     1021 ccatcacccg ggagatgatc gagatcgcct ccggggccga ggcccaggcg agctgatcca
     1081 gaccagaagg agcggctgaa gacccatggc ctccggcaag gtgatccaga tcacgggacc
     1141 cgtggtcgac gtcgaatttc ccgcgggcga gctgcccgag atctacaacg cgttggagat
     1201 cggcgagctc gtcgtcgagg ttcagcagca tctgggcaac aactgggtgc gggccgtggc
     1261 gatgtcgaca accgatgggc tcgcccgcgg cgtcgatgcg atcgacaccg ggaaggccat
     1321 caccgtcccc gtgggcgagc ccactctcgg ccgcgtgttc gacgtcctcg gccatccgat
     1381 cgacggcaag ggaccggtgg tttccgagca catgctcccc attcaccgtg aggcgcccgc
     1441 gttcgaggac atggagaccg aggctgccgt cttcgagacg gggatcaagg tcatcgacct
     1501 cattgccccg ttcaagcgcg gtggcaaggt cggcgtgttc ggtggagccg gcgtcggcaa
     1561 gacggtcatc atccaggagc tcattcgcaa catcgcggcc gagcacgggg gctactcggt
     1621 gttcgcgggc gtaggcgagc ggtcgcgcga ggggaacgac ctcatcggcg agatgaccga
     1681 gtcgggcgtc atcgacaaga cagtgatggt cttcggccag atgaacgagc cgcccggtgc
     1741 ccgccagcgg gtggggctga ccgctctgac catggccgag tacttccgcg acgtggaagg
     1801 tcgcgacatc ctgctgttca tcgacaacat cttccgcttc acccaggccg ggtccgaagt
     1861 gtcagcgctg ctcggccgga tgccatcggc ggtgggctac cagccaaccc tggccactga
     1921 gatgggcgac ctccaggagc gcatcacctc gaccaagaag ggctcgatca cctcgctgca
     1981 ggcgatcttc gtgccggccg acgactacac cgacccggca ccggccacca cgttcggcca
     2041 cctcgactcc accatccgcc tcgagcgctc catcgtggag ctgggcatct accctgcggt
     2101 ggacccgctg accagcacct cgaccgtgct cgatccgcgg gtggtgggcg aggaccactt
     2161 ccaggtggca cgcgaggtcc agcagaccct ccagcgctac cgcgacctcc aggacatcat
     2221 cgcgatcctg ggcatggacg agctgtcgga ggaggacaag gtgaccgtct cccgtgctcg
     2281 ccgcctacag cgcttcatga gccagccgat gttcgtcgcc gcccagttca ccgggcgcga
     2341 tgggaagtac gtcgagcgca aggagactgt ccgcggcttc cgcgagatcc tcgacggcaa
     2401 gcacgaccag ctccccgagc aggcgttcta catggt
//
LOCUS       DASYGU010000115         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_535373, whole genome shotgun sequence.
ACCESSION   DASYGU010000115 DASYGU010000000
VERSION     DASYGU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535373"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VGO32_03100"
     CDS             <1..722
                     /locus_tag="VGO32_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7199770.1"
                     /translation="VVLDAGTGIQRLGNELETGLQRIDILLTHLHMDHIQGLGFFAPI
                     FRRGCEIHVWGPPSPTLHLRARLTRYLSPPLFPIRLRDLRANFEFHDVPQGGFSIGPL
                     TIEADEVIHPGATLGYRITENGHALAYLSDHEPALATTNGNPGPAWVSGLALARRADV
                     LIHDAQYTQSERDERLGWGHSSVPEAIDFAHAAEARKLVLFHHDPARTDHQMDLLLAE
                     AEGLADGLPVTAAREGDVLVL"
     gene            807..1292
                     /locus_tag="VGO32_03105"
     CDS             807..1292
                     /locus_tag="VGO32_03105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV7199771.1"
                     /translation="MVLAAGVIGGGLAVVLLRNVIHSAVAMMFCFGALAGIYALLGAP
                     IVAAAQVLIYLGAISVLILFAIMLTQAGDANLPAPFQRQAPVAAVVAVLIVAVVGWTL
                     TQTEWPNGTEIAPVDLNELAIAIFNDYALPFEIVAFLLLAAIIGAIYLARRPEEDDRA
                     N"
     gene            1296..1604
                     /gene="nuoK"
                     /locus_tag="VGO32_03110"
     CDS             1296..1604
                     /gene="nuoK"
                     /locus_tag="VGO32_03110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV7199772.1"
                     /translation="MGLTAYLFLSLALFGIGAFGFLSRRNAIMMLICIELMLNAVNLA
                     LVAFNAFDAGAADDAGAVFSLLIMAVAAAEATVGLALVIAIIRTRQTAEVDDYSDLKE
                     "
BASE COUNT          244 a          618 c          537 g          313 t
ORIGIN      
        1 tggtcgtgct ggatgcgggg accggcatcc agcggctggg caacgagctc gagacggggc
       61 tccagcggat cgacatcctg ctcactcatc tccacatgga tcacatccag ggcctgggat
      121 tcttcgcccc catcttccga cgcgggtgcg agatccacgt ctgggggccc ccatcaccca
      181 cgctccactt gcgggcccgc ctcacgcgct acctgtcgcc accgttgttc ccgatccgcc
      241 tccgcgactt gcgggccaac ttcgagttcc acgacgtccc gcagggcggc ttctccatcg
      301 gtccgctgac catcgaagcc gacgaggtca tccatcccgg tgcgaccctg ggttatcgga
      361 tcacggagaa cggtcacgca ttggcctacc tcagcgacca cgagccggcg ctggccacca
      421 ccaatggcaa ccccggtccg gcctgggtca gcggcctcgc cctcgcccgg cgggccgacg
      481 tcctcatcca cgacgcccag tacacccagt ccgagcggga cgaacgcctg ggctggggcc
      541 actcctcggt gccggaggcg atcgactttg cccacgcggc cgaggcccgc aagctcgtcc
      601 tgttccatca cgatccggct cggaccgacc accagatgga ccttctcctc gccgaagccg
      661 aagggctggc cgatgggctg cccgtgacgg ccgcgcgcga gggtgacgtg ctcgtccttt
      721 agaatcgagc ccggcctccg aagcgaggcc ctgagcgctc ggtctcgaac ggagagtctc
      781 gactggacat ctcggctgtc ctcttcgtgg tgctcgccgc tggcgtgatt ggcggcggcc
      841 tggcggtggt gctgctgcgg aacgtcattc attccgcggt ggccatgatg ttctgcttcg
      901 gcgcgctggc gggtatttac gccctgctgg gtgccccgat cgtggccgcg gcccaggtgc
      961 tcatctacct gggcgcgatc agcgtcctga tcctgttcgc gatcatgctc acccaggccg
     1021 gcgacgcaaa cctgccggcg ccattccagc gccaggcgcc ggtcgccgcg gtggtcgccg
     1081 tcctgatcgt ggcggtcgtg gggtggacgc tgacccagac cgaatggccc aacggaaccg
     1141 agatcgcccc ggtcgacctg aacgagctgg cgatcgcgat cttcaacgac tacgccctcc
     1201 cgttcgagat cgtcgccttc cttctcctgg cggcgatcat cggcgccatc tatctggccc
     1261 gtcggcccga agaagacgac cgggcgaact gaccgatggg cctgaccgcg tacctcttcc
     1321 tgtcactggc cctgttcggc atcggggcgt tcgggttcct gtcccgccgg aacgcgatca
     1381 tgatgctgat ctgcattgag ctcatgctca acgcggtcaa cctggccctc gtggcgttca
     1441 acgcgttcga cgccggtgcc gccgatgacg cgggcgcggt gttctcgctc ctgatcatgg
     1501 ccgtcgcggc ggccgaggcc acagtcggcc tggccctcgt gatcgccatc atccgcaccc
     1561 gtcaaaccgc tgaggttgac gactactcgg acctcaagga gtagggcgct gaatcccgag
     1621 acactgatcc cgctgctgcc cgccctgccg ctggtcggct tcgtgttcgc ggtcctcctc
     1681 ggcccgaggt tggatcggat cccgagccat gg
//
LOCUS       DASYGU010000116         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1255479, whole genome shotgun sequence.
ACCESSION   DASYGU010000116 DASYGU010000000
VERSION     DASYGU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255479"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VGO32_03115"
     CDS             complement(<1..837)
                     /locus_tag="VGO32_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199773.1"
                     /translation="MTHDSLPAEQLRNAANRWGTPLYVTDLDAATARMDAYQSAFPGA
                     LVAYAVKANTDPRLLRRFAASGAGAEVVNAVELAFALSAGFTAERIVMNGLGKTDVDH
                     AAADEVGALVNAESLDELDLLLALDAARIGLRLNPGLEADTHAHLATGASNSKFGIAW
                     TDLDAAIDRCRAAGRAIENVGAHIGSAIDDLGAFTRLAELLAEAAARAEATRINLGGG
                     AGTSISVSDLADAVRADLPDPTRLILEPGRSLVADAGWLVTRVVRVQPRPEASLTYLV
                     ADA"
     gene            862..1356
                     /locus_tag="VGO32_03120"
     CDS             862..1356
                     /locus_tag="VGO32_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7199774.1"
                     /translation="MASRVSAGILLYRWRGDTLEVLLGHPGGPYFTNKGDGHWSIPKG
                     EADGGEVEAADLRLVARREFEEETGSPVPGALDDLLPLDEITQKGGKRVVAWAVEGDL
                     DPDTAESNLFSVEWPPRSGRFADYPEFDEVAWMTPNEARQRIKESQIPFIDRLEAALA
                     SARA"
     gene            complement(1360..>3124)
                     /locus_tag="VGO32_03125"
     CDS             complement(1360..>3124)
                     /locus_tag="VGO32_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199775.1"
                     /translation="GGEELESGLDRLVKRELLLRDDDPRSPERGQYRFLQAVVREVAY
                     ESLAKKDRRAKHLAVARHMESLGDDELAGVLASHYVEAYRATSAGPEADALGAQARIA
                     LRGAAERAAALHSHASAVHYLEEAVTVTTDPAEQAALYERAAESSTSLSSNDAVAHAT
                     QAIDLYRAAGDADGVLRATVLFARSGLLLGLGSRATSALENVLAVTPPDAEGWAAAMG
                     ELARAYMLTARSEEAVTTADQALAAIGGRRLPRLTADILITRGTALQSRPDEAVAILR
                     GAVALADRSGHIPTMIRGRNNLLTVLSGDITVDELDALCRETAELARRGGDARFLAQM
                     LLSIADTARDAGNWAECDAVLAELGAMDLDTFRMTWYLLSLAARQAFCSDLAGAEANL
                     ALADTVSTGVDTSAYGTKRAFIALVHIGMGRLDEAMEISIAAAAGPNQESSVPLIAIS
                     AAGAADPARAADLQADLDDAPSTRLNQVARRQVAALVAVSEQRWDDARAAFRAALEEF
                     EALGQVLYGSMAALQFEAYLGARFDDARQAGMAAATTLAEGDGTGFVERYRAAFPAMS
                     APPVSVVPAKARVPVDAEQPA"
BASE COUNT          474 a         1068 c         1118 g          464 t
ORIGIN      
        1 cgcgtcggcg accaggtagg tgaggctggc ctcggggcgg ggttggacgc ggacgacccg
       61 ggtcacgagc caccccgcgt ccgccaccag ggagcggccc ggctcgagga tgaggcgtgt
      121 gggatcgggg aggtcggcgc gcacggcatc ggcgagatcg ctcaccgaga tgctggtccc
      181 ggctccccca ccgaggttga tgcgggtcgc ctctgcgcgg gcggccgcct ccgccaggag
      241 ctccgccagg cgggtgaacg ccccgaggtc atcgatcgcc gacccgatgt gtgcgcccac
      301 gttctcgatc gcgcggccgg ccgcgcggca tcggtcgatc gcggcgtcca gatcggtcca
      361 ggcgatcccg aacttgctgt ttgaggcacc ggtggccaga tgcgcgtggg tgtcggcctc
      421 caggcccgga ttgagccgca gaccgatgcg cgccgcatcg agcgccagga gcagatcgag
      481 ctcgtcgagg gactcggcgt tgaccagggc cccgacctcg tcggcagcgg catggtcgac
      541 atcggtcttg cccaacccgt tcatcacgat ccgttcggcc gtgaagccgg cactcagggc
      601 gaaggccagc tcgaccgcat tcaccacctc ggcaccggct cccgaggccg cgaacctcct
      661 gagcagtcga ggatcggtgt tggccttgac cgcgtacgcc acgagcgcgc cggggaaggc
      721 cgactggtac gcgtccatcc gcgcagtcgc cgcgtcgagg tcggtgacgt agagcggcgt
      781 gccccatcgg ttcgccgcgt tccgcagctg ttcggcgggg agcgaatcgt gcgtcacgtc
      841 acggcatcct agactgcggc cgtggcaagc cgggtcagcg caggcatcct cctctaccga
      901 tggcgtggag acacgctcga ggtgctgctg ggccatccag gcggcccata cttcaccaac
      961 aagggcgacg gccactggtc gatcccgaag ggtgaagccg atggcggcga ggtcgaggcc
     1021 gccgatctcc gcctcgtcgc gcggcgcgag ttcgaggagg agaccgggag cccggtcccg
     1081 ggagcgttgg acgacctgct cccgctggac gagatcaccc agaagggagg gaagcgggtg
     1141 gtcgcttggg ccgtcgaggg tgacctggac cccgacacgg cggagagcaa cctgttcagc
     1201 gtggagtggc cgccgcgctc gggccggttc gcggactacc ccgagttcga cgaggtggcc
     1261 tggatgacgc cgaacgaggc tcgccagcgg atcaaggagt cccagatccc gttcatcgac
     1321 cggctggaag ccgcgctggc ctccgccagg gcctaaccct caggcgggct gctccgcatc
     1381 gaccggaacc cgtgccttgg cgggaactac ggataccggc ggggcagaca tggccggaaa
     1441 cgccgcccgg tagcgctcca cgaatccggt gccgtcaccc tcggcgaggg tagtcgccgc
     1501 cgccatgcca gcctgccgag catcgtcgaa ccgcgcgccc aagtacgcct cgaactggag
     1561 ggctgccatc gatccgtaga gcacctgccc aagggcttcg aattcctcca acgcagcccg
     1621 gaacgcggcc cgagcatcgt cccagcgttg ctcggaaacg gcaacgaggg ccgcgacctg
     1681 gcggcgggcg acctggttca ggcgtgtcga gggagcgtca tccaggtccg cctggagatc
     1741 cgcggcccgg gcaggatccg cggcgcccgc ggctgagatc gcgatgaggg gcacgctgga
     1801 ctcctgattc ggtccagcgg cagccgcgat gctgatctcc atggcctcgt ccagcctgcc
     1861 catcccaatg tggaccaggg cgatgaatgc gcgcttggtg ccgtaggccg acgtatcgac
     1921 tccggtgctg accgtgtcgg ccaatgcgag gttcgcttcc gctccggcta ggtcgctgca
     1981 gaacgcctgg cgagcggcca ggctgaggag gtaccaggtc atacggaagg tatcgaggtc
     2041 catggcaccc agctcggcaa ggaccgcgtc gcattccgcc cagttgcccg catctcgggc
     2101 cgtatcggcg atggagagca gcatttgggc gaggaatctg gcgtctccgc cgcggcgggc
     2161 cagctccgcg gtctcgcggc acagagcgtc cagctcatcg acggtgatgt cgccactcag
     2221 cacggtgagc aggttgttcc gcccacggat catggtcggg atgtggcccg agcgatcggc
     2281 cagcgcgacc gcgccgcgga ggatggcgac ggcctcgtcc gggcgcgact ggagggcagt
     2341 tccgcgcgtt atgaggatgt ccgcggtgag tcgcggcaag cgacggccgc cgatggcggc
     2401 cagcgcctgg tcggcggtgg tgaccgcctc ctcgctccgc gcggtgagca tgtatgcccg
     2461 cgccagctca cccatcgccg cggcccaccc ctctgcatcg gggggcgtga ccgcgagcac
     2521 gttctcgagg gccgatgtcg ctcgcgagcc cagacccagg agcagcccgg aacgagcgaa
     2581 tagcactgtc gctcggagca cgccgtcggc gtcgcccgct gcccggtaca ggtcgatcgc
     2641 ttgggtcgca tgcgcgacgg catcgttgct gctgagcgag gtcgaggatt ctgccgctcg
     2701 ctcatagagg gcggcctgct cagcgggatc ggtggtgacc gtgactgcct cttcgaggta
     2761 gtggaccgcg ctggcatggg aatggagcgc cgcggcccgt tcggcggcgc cacgcagcgc
     2821 gatccgggcc tgcgccccca gggcatcggc ctccgggccg gctgaagtcg cccgatatgc
     2881 ctcgacgtag tggctggcca ggaccccagc cagctcgtcg tcaccgagcg attccatgtg
     2941 acgggccacg gccaggtgct tcgcgcggcg atccttcttc gccagcgact cgtaggccac
     3001 ctcgcggacg acggcctgga ggaatcggta ctggccgcgc tcgggagagc gtggatcgtc
     3061 gtcccgaagc agcagctcgc gtttcacgag ccggtcgagc cctgattcca actcctcccc
     3121 gccc
//
LOCUS       DASYGU010000117         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_432919, whole genome shotgun sequence.
ACCESSION   DASYGU010000117 DASYGU010000000
VERSION     DASYGU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432919"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VGO32_03130"
     CDS             <1..761
                     /locus_tag="VGO32_03130"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HEV7199776.1"
                     /translation="PAQEQAVAELVTVVNDVAKSGGVLLIPAFAIGRTQHLVWLLDDL
                     VRSGRIPRLPLFVDSPMACEANDVYLRHPEGYDVETLALLQSGDSPLVYPGQACTTSV
                     EASKAIIGSPRPFILVAASGMLTGGRILHHLKDLLPDPRATLLFIGYQGEGTLGRHLQ
                     DGGKEARIDGASWPVHCRVQSITGLSAHADQTELDDWIGHFGAAGREDGRPRAVYVTH
                     GEPAAAAAFGSRISRDLGVRAEVPALGEEVTFPA"
     gene            788..1609
                     /locus_tag="VGO32_03135"
     CDS             788..1609
                     /locus_tag="VGO32_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEV7199777.1"
                     /translation="MSPRPVVSILTDFGARDPSGAICEGVILGICPDAAVIHISHEVT
                     KFAIRDGALMLWCAVPYLPVGVHMAVVDPGVGSARRPVAVVTRHGDVLIGPDNGLLMP
                     AAERLGGVVAAHELTSEAHRLHPVSTSFHGRDIFAPAVAHLANGVPIAELGPSVDSAT
                     LVALDIPPATPIAGGVATSVIYVDTFGNCKLAGMRADLEAAVGPMVSGRAVRIASGGA
                     GGRSMGWADTFGDVPVGEPMLYVDSYDRLTIAVNQGNAADALGLTQDQPLEILVA"
     gene            1620..3254
                     /locus_tag="VGO32_03140"
     CDS             1620..3254
                     /locus_tag="VGO32_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199778.1"
                     /translation="MTDPVAVVEGLSFRYQRRAEPALQDVSLEIAPGEVLLVAGPSGC
                     GKSTLIRVLNGLIPHAYRGDLTGTVRIGGEDTTVTPLRTLAMTVGTVLQDPARQLVAS
                     TVPAELTFGPENLGVERSEIGRRLVEVASASGLDPLMGRTTDELSGGEAQQVAIGGAL
                     MLAPRLLVLDEPLANLDPASASRLLGLLRRLADAGTAVVVVEHRIEDVLEARPDRALY
                     MEDGRVRYLGSMDGFLETADPTSIKLPFDAWLRRARSGARSVSEPPVPAAAADDGPPR
                     LEWRAVDAAYDGRAVLHGVSARLGRQERVAVLGPNGSGKTTLFKAAIGLRPLTGGDVL
                     VDGVSIAGRGVAELATTFGYVFQNPSQMLFAATVTDELLFGPRNLGRDPESFDSLVAE
                     SLARVGLSNEPEVGGRPPRTLSHGQQKRLAIAVALALEPRTLVLDEPSAGQDYRSATA
                     FMREVERIAGLESVYFVTHDVDLALTTADRIILLREGRIAADGTPREVVQDRERWRAC
                     NLRETSLMEANRQWSHVGTRFLGPEALADQLVQTPN"
BASE COUNT          479 a         1224 c         1183 g          501 t
ORIGIN      
        1 accccgccca ggagcaggcc gtcgcggagc tggtcacggt cgtgaacgac gtggccaaga
       61 gcgggggcgt gctcctcatc ccggccttcg ccatcggccg cacccagcac ctcgtctggc
      121 tcctcgacga cctggtccga agcggccgga tcccgcgcct gccgctgttc gtcgactcgc
      181 cgatggcctg cgaggccaac gacgtgtatc tccgccaccc ggagggctac gacgtggaga
      241 ccctggccct gctccagtcg ggcgattcgc cgctggtcta tcccggccag gcgtgcacga
      301 ccagcgtcga ggcctccaag gcgatcatcg gcagcccgcg gccgttcatc ctggtcgccg
      361 cttcggggat gctgaccggc ggtcggatcc tccatcacct gaaggacctg ctgcccgatc
      421 cacgtgccac cctgctgttc atcggctacc agggcgaggg caccctgggc cggcatctcc
      481 aggacggcgg gaaggaggcg cgaatcgacg gcgcgtcatg gccggtccac tgtcgggtgc
      541 agtccatcac cggcctctcc gcccacgccg atcagaccga gctcgacgac tggatcgggc
      601 acttcggcgc ggcgggccgg gaggacgggc gaccgcgcgc ggtgtacgtc actcacggcg
      661 agcccgcagc cgccgcggcc ttcggatcgc ggatcagtcg cgatctgggc gtcagggccg
      721 aggtgcctgc cctcggggaa gaggtcacct tccccgcctg acccgggtgc ggctaccatc
      781 cgggcctatg tcgccccgac ccgtggtcag catcctcacc gacttcggcg cgcgcgaccc
      841 atcgggtgcg atctgcgagg gtgtcatcct cggcatctgc cccgatgcgg cggtgatcca
      901 catcagccac gaggtgacca agttcgccat ccgggacggg gccctcatgc tgtggtgcgc
      961 cgtgccgtac ctccctgtcg gcgtccacat ggcggtggtc gatccggggg tcgggagcgc
     1021 gcgccgaccc gtcgccgtcg tgacacggca tggggacgtc ctgatcggtc cggacaatgg
     1081 gctcctcatg ccggccgccg agcgcctggg tggcgtggtc gccgcccacg agctgacgag
     1141 cgaggcgcac cggctccacc cggtgagcac cagcttccac ggccgggaca tcttcgcgcc
     1201 ggccgtcgcc cacctcgcca acggcgtgcc gatcgcggag ctcggaccgt cagtcgactc
     1261 cgcgaccctg gtggccctcg acatcccgcc cgccaccccg atcgcgggtg gcgtggccac
     1321 gagcgtcatc tacgtcgaca ccttcggcaa ctgcaagctg gcgggcatgc gcgccgacct
     1381 ggaggcggcg gttggaccga tggtctccgg gcgcgcagtg cggatcgcgt ccggcggcgc
     1441 gggcgggcgg tccatgggat gggccgatac cttcggggac gtgccggttg gtgagccgat
     1501 gctgtacgtc gactcgtacg accgactcac gattgcggtc aaccagggca acgcggcgga
     1561 tgccctcggg ctcacccagg accagccgct cgagatcctc gtcgcgtgac gcacccggcg
     1621 tgaccgaccc ggtcgccgtc gtcgagggcc tgtcattccg gtaccagcgg cgcgccgagc
     1681 cggcgttgca ggacgtgtcg ctcgagatcg cgccgggtga ggtgctgctg gtcgccggtc
     1741 cctcgggctg cggcaagtcc accctcatcc gggtgctgaa cggcctgatc ccgcatgcct
     1801 accgcggtga cctgaccggc acggtgcgga tcgggggcga ggacacgacg gtgacccccc
     1861 tgcgcaccct ggccatgacg gtcgggaccg tcctccagga ccccgcccgc cagctggtgg
     1921 cctcgaccgt gcccgcggag ctgacgttcg gcccggaaaa cctcggcgtc gagcggtcag
     1981 agatcggtcg ccggctggtg gaggtggcca gcgccagcgg cctcgatccg ctcatgggtc
     2041 gcaccaccga cgagctgtcg ggtggcgagg cccagcaggt cgcgatcggc ggggcactca
     2101 tgctcgcccc ccggcttctc gtcctcgacg agccgctggc caacctcgat ccggcatcgg
     2161 ccagccgcct cctgggactc cttcgccgac tcgccgatgc gggcaccgcg gtggtcgtgg
     2221 tcgagcaccg gatcgaagac gtgctcgaag cgcgcccgga ccgcgccctg tacatggagg
     2281 acggccgggt ccgctacctc ggctcgatgg atggcttcct cgagacggcg gacccgacga
     2341 gcatcaagct gccgttcgat gcctggctga ggcgcgcgcg gtccggtgcg cggtcggtga
     2401 gcgaaccacc cgtgccagcc gccgcggcgg acgatggccc gccccggctg gagtggcgcg
     2461 ccgtggacgc ggcgtacgac gggcgggcgg tgctccatgg cgtctcggcc cgcctcggcc
     2521 ggcaggaacg ggtcgccgtc ctcgggccca acggatcggg caagaccacg ctgttcaagg
     2581 cggccatcgg actgcggcca ctgaccggcg gcgacgtgct ggtggacggc gtgtcgattg
     2641 ccgggcgcgg cgttgccgag ctagcgacca ccttcggcta cgtgttccag aacccgtccc
     2701 agatgctgtt cgcggccacg gtcacggacg agctcctgtt cggtccgcgg aacctcggcc
     2761 gcgaccccga atccttcgac agcctcgtcg cggagtcgct cgcgcgcgtg ggtctgagca
     2821 acgagccgga ggtcggcgga cggccgccgc ggaccctgtc ccacggccag cagaagcggc
     2881 tcgcgatcgc ggtcgcgctc gcgctcgagc cacgcacgtt ggtgcttgac gaaccgtcag
     2941 ccgggcagga ctatcgaagc gccaccgcgt tcatgaggga ggtggagcgc atcgcaggat
     3001 tggagagcgt gtacttcgtc acccacgacg tcgatctcgc cctgaccacc gccgaccgga
     3061 tcattctcct gcgagaaggg cggatcgcgg ccgatgggac cccgcgcgag gtcgtgcagg
     3121 atcgggagcg gtggcgagcc tgcaacctgc gggaaacctc gctcatggaa gccaaccggc
     3181 aatggagcca cgtggggacc aggttcctgg gccccgaagc cctggccgat caactggttc
     3241 agaccccgaa ctgaccccga ggaggagcat tcgcccatga ccgctgtcgc acagaccccg
     3301 ccaagcccga gtccctgggc catcacgacc cgggttatcg tgtacgccgc catcggcgcg
     3361 gcgctgtacg ccatcttcaa ctggatc
//
LOCUS       DASYGU010000118         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_21343, whole genome shotgun sequence.
ACCESSION   DASYGU010000118 DASYGU010000000
VERSION     DASYGU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21343"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VGO32_03145"
     CDS             <1..830
                     /locus_tag="VGO32_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199779.1"
                     /translation="GLIHPDHGSIELLGRPFGRRDRRRLFEIGALIESPSFYPFLTGR
                     ENLRALGSTGAKTSAARIDELLELTGLRERSTDKVSGYSLGMKQRLGIAAALLSDPKL
                     LLLDEPANGLDPAGIVAMRETLRHLAADGKTVFVSSHLLAEVQQMADVIGIIAAGRLV
                     REGSMGELLHDTDLVRVRVAPDEVADAVARLSQLAGESQVEVADAEAGWLTVRIASGR
                     APDVNRVLAEGGVYASRLEAGSDLEELFLSLTADESESHEGTFQGIASARRDPEDTP"
     gene            827..1618
                     /locus_tag="VGO32_03150"
     CDS             827..1618
                     /locus_tag="VGO32_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7199780.1"
                     /translation="MRIFRSGARTLLRRPATWLTFGILTGLLVLIIVAVATTGEDAED
                     GPDRLLLVTFPLAYTFILSFIIGLGGLFAVTYGAAIAGSEWSWGTLKAVVARGESRSR
                     YILLTFSAVAAYLVAGIAFSFAIGVVAAFIGANIAGVPTDGMTDTDAIESLPELFSRG
                     WFSMAEQGAIGFAVATVARSQLAGIGIGIALYFGEAFAGIFLPDIVRFMPFNAASAAV
                     GIADGGFGGGPNPLAQDTAMFVVLAWLVGSLIVAAVFTERADITG"
     gene            1623..>2169
                     /locus_tag="VGO32_03155"
     CDS             1623..>2169
                     /locus_tag="VGO32_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEV7199781.1"
                     /translation="MTLLAVSNFLAILVVVAGVGSLVVIGLRLTAVFSERAAGGLDRL
                     RAAIGPYALWLAWLVAATAMAGSLYFSELGGLMPCALCWYQRIAMYPLAVILLIAAIR
                     GDGGIRPYVTVLAAIGALIAGYHVLVQRIPGLPTGSCSVEASCSAIDLERFGFITIPV
                     MALVGFLTILVLVGILASPTDE"
BASE COUNT          284 a          761 c          738 g          386 t
ORIGIN      
        1 ccgggctcat tcatccggac cacggcagca tcgagctcct gggccgcccg ttcggccgtc
       61 gtgaccgccg gcgcctgttc gagatcggcg ccctgatcga gtctccgtcg ttctatccct
      121 tcctgaccgg ccgcgagaac ctgcgagcgc tgggctcgac cggcgcgaag acatcggctg
      181 cgcggatcga cgagctgctc gagctcaccg gtcttcgcga gcggtccacc gacaaggtct
      241 ccggctactc gctgggcatg aagcagcggc tcgggatcgc ggccgcgctg ctcagtgacc
      301 cgaagctgct cctcctcgac gagccggcca acgggctcga cccggccggg atcgtggcca
      361 tgcgcgagac cctccgtcat ctcgcggcag acgggaagac ggtcttcgtc tcgagccacc
      421 tcctggccga ggtccagcag atggccgacg tgatcgggat catcgccgcc ggtcgactgg
      481 tccgagaggg atccatgggc gagctcctcc acgataccga cctggtgcgg gttcgggtgg
      541 ccccggacga ggtggcggac gccgtcgccc ggctgtccca gctggccgga gagtcgcagg
      601 tcgaggtcgc cgacgcggag gccggatggc tcacggtccg catcgcctcg ggccgggcgc
      661 cggacgtcaa ccgggtgctc gccgagggcg gggtgtacgc cagccgtctc gaggcgggca
      721 gcgatctcga ggagctgttc ctgtcgctca cggcagacga atcggagagt cacgagggca
      781 ccttccaggg catcgccagc gcccgtcgcg accccgagga cacgccatga ggatcttccg
      841 ctcaggggcg cggaccctcc tgcgccggcc ggcgacatgg ctgaccttcg gcatcctcac
      901 cggcctgctg gtcctgatca tcgtggccgt ggccaccacc ggcgaggacg ctgaagacgg
      961 gcccgatcgc ctgctgctgg tcaccttccc gctcgcgtac accttcatcc tcagcttcat
     1021 catcggcctg ggcgggctat tcgcggtgac ctacggagcc gccatcgcgg gctcggaatg
     1081 gtcatggggg acgctcaagg ccgtggtcgc ccgcggcgag agtcggagcc gatacatcct
     1141 gctcaccttc tccgccgtgg ccgcatacct cgtggccggc atcgcctttt cgtttgcgat
     1201 cggcgtggtg gccgcgttca tcggcgccaa catcgccggc gtgccgaccg atggcatgac
     1261 cgataccgat gcgatcgaga gcctgccgga gctgttcagc cgcggctggt tctccatggc
     1321 cgagcagggc gccatcgggt tcgcggtggc gaccgtcgcg cgcagccagc tggcggggat
     1381 cgggatcggg atcgcactgt acttcggcga ggcgttcgcg gggatcttcc tgcctgacat
     1441 cgtccgcttc atgcccttca acgccgccag cgcggcggtg ggaatcgccg atggcgggtt
     1501 tggcggcggt ccgaacccgc tggcgcagga caccgccatg ttcgtggtcc ttgcctggct
     1561 ggtcgggtcg ctgatcgtgg ccgcggtctt caccgagcgc gctgacatca ccggctgacc
     1621 gggtgaccct gctcgccgtc tcgaacttcc tcgccatcct ggtcgtcgtg gcgggagtcg
     1681 gcagcctcgt cgtgatcggt ctccgcctga ccgcggtctt ctcggagcga gcggctggcg
     1741 gcctcgaccg tcttcgcgcc gccatcggcc cgtacgcgct gtggctggca tggctggtcg
     1801 ccgccaccgc gatggccggc tcgctgtact tctcggagct cggcggactc atgccctgcg
     1861 cgttgtgctg gtaccagcgg attgcgatgt acccgctcgc cgtcatcctg ctcatcgccg
     1921 ccatccgcgg cgatggggga atccggccgt acgtgacggt gctcgcggca atcggtgcgc
     1981 tgatcgccgg ataccacgtc ctcgtgcaac ggatcccggg cctgccaacc gggagttgtt
     2041 ccgtcgaggc atcgtgctcg gccatcgatc tcgagcgctt cggcttcatc acaatcccgg
     2101 tcatggccct ggtcggattc ctgaccatcc tcgtgctggt gggaatcctg gcctccccaa
     2161 cggacgagc
//
LOCUS       DASYGU010000119         4954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_227096, whole genome shotgun sequence.
ACCESSION   DASYGU010000119 DASYGU010000000
VERSION     DASYGU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227096"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VGO32_03160"
     CDS             complement(<1..1843)
                     /locus_tag="VGO32_03160"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEV7199782.1"
                     /translation="MGATIGHAAVVIGLALTLFAAAASILAGRSGDAGLATVGRRAVY
                     GAWGLSVIACAAMVVSLLGHDFSVLYVARNNATTTPPFYSFISLWAALEGSILFWTFL
                     LTGWSSLLLYRYRHAHPELMPWATATLALVAAFFFAVMTWPSDPFVAVTPVTVEGNGP
                     NALLQNHPFMGLHPPLLYLGYTGLAIPFALTVAALVTRRLDPEWLRLVRRWTLGPWIF
                     LTAGITAGAWWSYEVLGWGGYWAWDPVENAALLPWLTATAFIHSAIVAERGRSLRTWT
                     AILVMTTFVLTLLGTFLTRSGVIESVHSFTQSAIGAWFLAGVLISLVAGTALILWRLP
                     DLAGSGPPPALVSRESAFLLNNLLFTGLAFAVLFGTLYPLVAEALGGERLSVGAPWFD
                     RISAPFFAALLFLLGVGPALPWGSVSWPALRDRFVLPAVAGVGLMVVGIVIGVRGPAS
                     LLTLGLAGFSGAILVGEVVRAARGRAAASGENSLRAAWGVATRNRRRYGGYLAHVGVL
                     VAAIAVAVSATQAVEATAILAPGESTRLGPYTVTHDQLIREPLASDPRVFETRAELEV
                     QGPQAGQVSPALRDYPNSVTAIATPSVMTSAGEDFYLTLLAYDVGSGT"
     gene            complement(1847..2278)
                     /locus_tag="VGO32_03165"
     CDS             complement(1847..2278)
                     /locus_tag="VGO32_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEV7199783.1"
                     /translation="MTASAPLPPPARHRAWGPVLVVVGLLAILAWLLFSGLSNALVYY
                     LTPTELAARGDAAVGQAIRLGGYVLPGTLDGSATDLTFVLTDGQTEITVHSTVTPTSS
                     FREGAGAVVEGTLQEDGVFEADEVLVKHDENYVAPSDFPVP"
     gene            complement(2275..2418)
                     /locus_tag="VGO32_03170"
     CDS             complement(2275..2418)
                     /locus_tag="VGO32_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199784.1"
                     /translation="MSDVVFVISAYGVILGAIAIYAFTLIRRLRRAQQAADELEETPN
                     PAP"
     gene            complement(2421..3137)
                     /gene="ccsA"
                     /locus_tag="VGO32_03175"
     CDS             complement(2421..3137)
                     /gene="ccsA"
                     /locus_tag="VGO32_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEV7199785.1"
                     /translation="MATRVLGIGAVVAVAGATAFSLFVVPADLNQGEPQRIMYLHVAS
                     AWFAYLSFVITAVASIWWLVRRDPRADAVALAGAEVGVVFTASTIWAGMMWGRPVWGT
                     FWDWGDPRLTTTAVLLALYIGYLLIRRLTDEPVRRATRAAVVGIIAAIDIPVVHFSVI
                     WWRGLHQGPTFGDPEHVLNPAAPSQFVAALLLMLGAFSLAWLWLMLKRYRLARLEWDA
                     EDAVRRSGLTGLAAASEPGS"
     gene            complement(3144..3833)
                     /locus_tag="VGO32_03180"
     CDS             complement(3144..3833)
                     /locus_tag="VGO32_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEV7199786.1"
                     /translation="MRTATHNLSVAGVIAAKDLRAELRGRQAVTSTLFLAAVTMLIFG
                     FALGPDEDRLAVAGPGLLWLAIILSGILAVGRLHHLETDDGAFELLGLYPISRTGIYL
                     GKALGGLAAMLVIGALVLPLTVILFAVDVASAVPGLLLVIALGSIGFAAVGTLYAGLT
                     VRLRAREVLLPLLLLPVVAPLLLAAVSATALLLAGDPFGELPAWLQLLAGYDLAMLLA
                     GGLTYGLALEE"
     gene            complement(3830..4615)
                     /gene="ccmA"
                     /locus_tag="VGO32_03185"
     CDS             complement(3830..4615)
                     /gene="ccmA"
                     /locus_tag="VGO32_03185"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEV7199787.1"
                     /translation="MPPTTIHVRPTGVPDKTTPGVETRDLARLFRRSPALAGVTIQVV
                     SGRAVAILGANGAGKTTLLRVLATALRPSYGSAAVDGIDVADRPEEIRPRVAFLPHAT
                     GLYDDLTAAENLGFAAALMNVPAPDREDRVGAALERVHLTTERDSRVGRFSAGMRRRL
                     ALGRILLGHPRLVLLDEPYAALDAEGMALVDELLDEWRSNGATVLVATHATERLGERM
                     DGWMRLENGLVVDLGGVGVIGRVAARPATDLPASPPLAAGFRS"
     gene            4705..>4954
                     /locus_tag="VGO32_03190"
     CDS             4705..>4954
                     /locus_tag="VGO32_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199788.1"
                     /translation="MSTPRSACRFRASFGLVLVLVGAVSLAACSPSSSPAADADHTVT
                     ITGTSFGDDFTVPVGGTVVFVNDAGRNHTVTNGVNGEPN"
BASE COUNT          842 a         1707 c         1810 g          595 t
ORIGIN      
        1 ccgtgccgct gcccacgtcg taggcgagga gggtgaggta gaagtcctcg ccagcggagg
       61 tcatcacgct gggggtggcg atggccgtca ccgagttggg gtagtcgcgg agagcggggc
      121 tcacctgccc ggcctgtggc ccctggacct ccagctcggc gcgggtctcg aagacgcgtg
      181 ggtcgctggc cagcggctcg cggatgagct ggtcgtgggt caccgtgtac ggacccagcc
      241 gggtggactc gccgggtgcc aggatcgcgg tggcctcgac cgcctgggtt gcgctcactg
      301 cgaccgcgat ggccgcgacc aggaccccta cgtgggccag gtatccccca tatcgccgcc
      361 ggttgcgggt ggccaccccc caggccgccc ggagcgagtt ctctccgctg gccgcggccc
      421 gtccgcgggc ggcgcgaacg acctcgccca ccaggatcgc gccggagaac ccggccaggc
      481 ccagggtcag cagagaggcc gggccccgga ccccgattac gatcccgacc accatgagcc
      541 cgaccccggc cacggcgggc agcacgaacc gatcgcggag cgcgggccag ctgaccgatc
      601 cccacggcag cgccggtccc acgcccagca ggaagaggag cgccgcgaag aatggcgcgc
      661 tgatccggtc gaaccacggc gcgccgacgc tgagccgttc tccgcccagc gcctcggcca
      721 ccagcggata gagcgtgccg aacaggaccg cgaaggcgag accggtgaac agcaggttgt
      781 tcaggaggaa tgccgactcg cggctcacga gggcaggcgg agggccgctg ccggccaaat
      841 cggggaggcg ccacaggatg agagcggtcc cggcgacaag cgagatcagg acaccggcca
      901 ggaaccaggc gccgatcgcg gactgggtga acgaatggac cgattcgatc acgcccgacc
      961 gggtgaggaa cgtccccagc agggtgagga cgaaggtggt catgaccagg atcgcggtcc
     1021 acgttcggag cgaccggccg cgctcggcca cgatcgccga gtggatgaac gcggtggccg
     1081 tgagccacgg caggagggcc gcgttttcga cgggatccca cgcccagtag ccaccccagc
     1141 ccaggacctc gtagctccac cacgccccgg ccgtgatccc cgcggtgagg aagatccacg
     1201 gacccagcgt ccagcggcgg acgaggcgca gccattcggg gtccaggcgg cgggtgacga
     1261 gtgcggcgac ggtcagcgca aacgggatgg cgagcccggt gtagcccagg tacaggagcg
     1321 gcgggtgaag gcccatgaac gggtggttct ggaggagggc attcggaccg ttgccttcga
     1381 ccgtgaccgg ggtgaccgcc acgaacgggt ccgacggcca ggtcatgacc gcgaagaaga
     1441 acgcggccac gagggccagg gtggccgtcg cccacggcat gagctccgga tgggcgtggc
     1501 ggtatcggta caggaggagg ctggaccaac cggtcaggag gaacgtccag aacaggatgc
     1561 tgccctcgag cgcggcccac agcgagatga acgagtagaa cggcggggtg gtcgtcgcgt
     1621 tgttgcgggc cacgtacagg accgagaagt cgtgaccgag gagcgagacg accatggccg
     1681 cgcaggcgat caccgacaga ccccaggccc cgtacacggc ccggcggccg acggtcgcca
     1741 atcctgcgtc accgctgcgg ccggccagga tcgaggccgc cgccgcgaac agggtgaggg
     1801 ccaggccgat gacgacggcc gcgtggccga tggttgcgcc catcggtcag ggcacgggga
     1861 agtcggaggg cgccacgtag ttctcgtcgt gcttgaccag cacctcgtcg gcctcgaaca
     1921 cgccgtcctc ctggagcgtg ccctcgacca cggcgccggc tccttcgcgg aacgagctgg
     1981 tgggagtcac ggtcgagtgg acggtgatct ccgtctggcc gtcggtgagg acgaaggtca
     2041 ggtcggtggc cgagccgtcc agcgttccgg gcaggacgta gccgccgagg cggatggcct
     2101 ggccgacggc ggcatcaccg cgggccgcga gctcggtcgg ggtgaggtag tagaccagcg
     2161 cgttgctcag cccgctgaac agcagccagg ccaggatggc cagcaggccg acgaccacga
     2221 gcaccggtcc ccaggcgcgg tgtcgcgcgg ggggcggaag cggggcggat gcggtcatgg
     2281 ggcggggttg ggggtctcct ccagctcgtc cgccgcctgc tgggctcgcc gcaggcggcg
     2341 gatgagagtg aaggcataga tcgcgatcgc gcccaggatc acgccgtagg ccgagatgac
     2401 gaacacgacg tcgctcacgt tcacgagcct ggctccgaag cggcggcgag gccggtcaat
     2461 ccgctgcggc ggacggcatc ttccgcgtcc cactcgagcc gggccaggcg atagcgcttg
     2521 agcatgagcc acagccaagc gaggctgaac gcgcccagca tgagcaggag cgcggccacg
     2581 aactggctcg gtgccgccgg gttcaggaca tgctcgggat caccgaacgt gggtccctgg
     2641 tgcaggcccc gccaccagat gacgctgaag tggaccaccg ggatgtcgat cgcggcgatg
     2701 atcccgacca ccgccgcccg ggtggcccgc cgcacgggct cgtcggtcag gcgccgaatg
     2761 agcagatagc cgatgtagag ggccaggagc acggcggtcg ttgtcagccg agggtcgccc
     2821 cagtcccaga aggtgcccca cacgggacgc ccccacatca tcccggccca gattgtcgag
     2881 gcggtgaaca cgacaccgac ctcggccccg gccagggcaa cggcgtccgc ccgtgggtcg
     2941 cgtcggacga gccaccatat ggatgcgacc gcggtgatca cgaacgagag gtacgcaaac
     3001 caggcgctgg ccacgtgcag gtacatgatc cgctgcggct cgccctggtt gagatcggcc
     3061 gggaccacga acagggagaa ggcggttgct cccgccacgg ccacgacggc accgatcccc
     3121 agcacccggg tcgccatcgg tcgttactcc tccagcgcca ggccgtaggt caagccgccg
     3181 gcgagcagca tggccaggtc gtagccggcc agcagctgga gccacgccgg cagctccccg
     3241 aaggggtcac ccgccaggag cagggccgtg gcgctgaccg cggccagaag gagcggcgcg
     3301 accaccggaa gcaggagcag cggaagaagg acctcgcgag cgcggaggcg gaccgtaagt
     3361 ccggcgtaca gcgttcccac agccgcgaac ccgatggacc ccagggcgat cacgagcagc
     3421 agaccgggca ccgcgctggc cacgtccacc gcgaacagga tcacggtcag gggcagcacc
     3481 agggcgccga tgaccagcat cgccgcgagc ccgcccagcg ctttgcccag atagatcccc
     3541 gtccggctga tggggtacag ccccaggagc tcgaacgcgc cgtcatctgt ctcgaggtgg
     3601 tggagccgac caacggccag gatcccgctc aggatgatgg ccagccacag gaggcccggc
     3661 ccggccaccg ccaggcggtc ctcgtccgga ccgagcgcga acccgaagat gagcatcgtc
     3721 acggcggcca ggaagagagt ggaggtcacc gcctgccggc cccgcagctc ggcgcggagg
     3781 tccttggcgg cgatcacgcc ggccaccgac aggttgtggg tggcggtcct cacgaccgga
     3841 acccggcagc caggggcggc gaagcgggca gatcggtggc gggccgggcc gcgacccgtc
     3901 cgatgacgcc gactccaccg aggtcgacca cgagcccgtt ctcgagccgc atccagccgt
     3961 ccatccgctc ccccagccgc tcggtggcgt gggtcgcgac caggaccgtg gcgccgttcg
     4021 accgccattc gtcgagcagc tcgtcgacga gagccatccc ttcggcgtcg agggctgcat
     4081 acggctcgtc cagcagcacg agccggggat ggccgagcag gatccgacca agtgccaggc
     4141 gccggcgcat gccggccgag aaccgaccca cccggctgtc gcgctccgtc gtgaggtgga
     4201 cccgctcgag ggccgcgccg acgcggtctt cccgatccgg ggcggggacg ttcatcagcg
     4261 cggccgcgaa gcccaggttc tcggcggcgg tgaggtcgtc gtacaggccc gtggcgtggg
     4321 gcaggaatgc aacccgcggg cggatctcct ccgggcggtc ggcgacatcg atgccgtcca
     4381 ccgcggccga cccgtacgag gggcgcaagg cggtcgccag cacgcgtagg agcgtggtct
     4441 tgccggcgcc gttggcgccc agaatggcca ccgcacggcc cgaaaccacc tggatcgtga
     4501 cccccgccag cgcagggctg cgtcggaaga gccgggccag gtcgcgagtc tcgactcccg
     4561 gcgtggtctt gtcagggacc cccgtcgggc gaacgtggat ggtggtcggc ggcatgcctc
     4621 atcccgtggg cggaggggcg ggtggcagca gtgtgcggcc tgccccacaa ttgcgtcaaa
     4681 taccccaagg gagctccgcg acccatgtcc accccacgtt ccgcctgccg tttccgcgcc
     4741 tcattcggcc tcgtcctggt gctcgtcggc gccgtctcgc tggcagcctg cagcccgtcc
     4801 tcctccccgg ccgctgacgc cgatcacacg gtgaccatca ccgggacgtc gttcggcgac
     4861 gacttcaccg tcccggtcgg cgggacggtc gtgttcgtga acgacgccgg gcgcaaccac
     4921 accgtaacca acggggtcaa cggcgagcca aacg
//
LOCUS       DASYGU010000120         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1770977, whole genome shotgun sequence.
ACCESSION   DASYGU010000120 DASYGU010000000
VERSION     DASYGU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770977"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGO32_03195"
     CDS             complement(<1..256)
                     /locus_tag="VGO32_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7199789.1"
                     /translation="MAASPTPVRLHNSLTRRVELLDPVQPGRVGIYTCGSTVYRYAHI
                     GNLRTYLFGDLLRRTLEYLGYEVTYVKNITDVGHMRDDATD"
     gene            302..895
                     /locus_tag="VGO32_03200"
     CDS             302..895
                     /locus_tag="VGO32_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7199790.1"
                     /translation="MPEHDASTSYPLTLGEDPAFCPACGKPVVERVLEEDHRPRLVCP
                     DGHVTWRNPRLVVGTLPVRDGQVFLARRGIEPGIGLWSYPGGFLEVGESAQEGARRET
                     EEETDLRVEIGRLIGAYSRPHGGVVTLIYEAQVVGGEALPAAETTEVRGFTPDAIPWD
                     ELAFSTTESCLRDWLLDQPGHERRHEPELYVVGDPEP"
     gene            complement(911..2200)
                     /gene="fabF"
                     /locus_tag="VGO32_03205"
     CDS             complement(911..2200)
                     /gene="fabF"
                     /locus_tag="VGO32_03205"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV7199791.1"
                     /translation="MTHTGRRAMVTGLGVLSPVGIGNDTAWANLVAGKSGIREVTVAD
                     LSDQDIKIGGEVPDFDPTREMDPKDVRRHDRATQMAVAATAEALRDAGLINGHSQILA
                     EEADPDRIGMVFGSGCGGVSILLDNAKKYWDVGANRVSPWTIPHMLVDSPSGMVAIQF
                     GIRGPNTAVVSACATGSHAIGEAAETIIRGQADLMLGAGTEAALVPLAFAGFGQMRAL
                     GTPIDPDTGEYVPSIASRPFDRTRNGFVIAEGCAVLVLEELEHALARGARPIAEVVGY
                     GSAADAYHMAAPPERGEGSQRSMRWALERGGIDPTEIDYINPHGTGTPLNDLSETQAI
                     EAVFGDHARKLAISSTKSMTGHMMGAAGAFEGWASVKVLDTGWIPPTINLNHPDPELT
                     LDYVPHVARQADVRVALSNSMGLGGHNGTIVFRKYEA"
     gene            complement(2197..>2473)
                     /locus_tag="VGO32_03210"
     CDS             complement(2197..>2473)
                     /locus_tag="VGO32_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199792.1"
                     /translation="TMIRTADGLRDELSEHLVHGVQWVATIRNMAAIGVTDFVEIGPG
                     RVLTGLIKRISPESGAHALDDADDGWLPSPQPSSGSKGASKPPRKKA"
BASE COUNT          344 a          832 c          889 g          408 t
ORIGIN      
        1 tatcggtcgc gtcgtcgcgc atatgcccga catcggtgat gttcttgacg tacgtgacct
       61 cgtagccgag gtactccagg gtccggcgga gcaggtctcc gaacaggtag gtccgcaggt
      121 tgccgatgtg cgcgtaccga tacaccgtgc tgccgcaggt gtagatcccg acccggcccg
      181 gctgcaccgg atcgaggagc tccacccggc gggtgagcga gttgtggagg cgaacagggg
      241 tgggtgatgc ggccatcagg gcccggattg tatcggtcgg gatcgggcta ccatctgacc
      301 gatgcccgaa cacgacgcga gcacgagcta cccgctgacc ctcggcgagg atccggcctt
      361 ctgccccgca tgcggcaagc cggtcgtcga gcgggtgctg gaggaggatc accgcccacg
      421 actggtctgc cctgacggcc acgtcacgtg gcgcaacccg cggctggtgg tgggcaccct
      481 cccggtccgc gacggccagg tgttcctcgc tcgtcgcggg atcgagccgg gcatcgggct
      541 gtggagctat ccgggcggct tcctggaggt cggcgagtcg gcccaggagg gggctcgccg
      601 cgaaaccgag gaggagaccg atctgcgagt cgagatcggt cgcctgatcg gcgcgtactc
      661 ccgtccgcac ggtggggtgg tgaccctcat ctacgaggcc caggtggtgg gcggcgaggc
      721 actgcccgcc gccgagacga ccgaggtccg aggatttacg ccggacgcca tcccgtggga
      781 cgagctcgcc ttcagcacga ccgagagctg cctgcgcgac tggctgctgg atcagcccgg
      841 gcacgagcgc cggcacgagc cggagctgta cgtggtgggg gacccggagc cctagccgcc
      901 gtcgggtcga tcaggcctcg tacttgcgga acacgatcgt tccgttgtgg ccgccgaggc
      961 ccatggagtt cgagagcgcg accctgacgt cggcctgtcg tgcgacgtgc gggacgtaat
     1021 cgagggtcag ctcggggtcg gggtggttca ggttgatcgt gggcgggatc cagccggtgt
     1081 ccagcacctt caccgatgcc cagccctcga acgcgcccgc cgcgcccatc atgtggccgg
     1141 tcatcgactt cgtcgagctg atcgcgagct ttcgggcgtg gtccccgaac acggcctcga
     1201 tggcctgcgt ctcggacaag tcgttgagcg gggtgcccgt gccgtggggg ttgatgtaat
     1261 cgatctcggt cgggtcgatc cctccccgct cgagcgccca gcgcatcgat cgctggctgc
     1321 cctcgccgcg ctcgggtggc gcggccatgt gatacgcgtc ggccgcgctg ccgtagccga
     1381 cgacctccgc gatcgggcgt gctccgcgag cgagcgcatg ctccagctcc tcgagcacga
     1441 gcaccgcgca cccctcggcg atgacgaacc cattgcgcgt tctgtcgaac ggccgcgagg
     1501 cgatggaagg cacgtactcg ccggtgtcgg ggtcgatcgg cgtgccgagc gcccgcatct
     1561 ggccgaagcc ggcgaacgcg agcgggacga gcgccgcctc ggtgcccgcg ccgagcatga
     1621 ggtcggcctg gccgcggatg atcgtctcgg ccgcttcgcc gatcgcgtgg ctgccggtcg
     1681 cgcacgcgct caccacggcg gtattcgggc cgcgaatacc gaactggatg gccaccatcc
     1741 ccgacgggct gtcaaccagc atgtggggga tggtccatgg cgatacccgg ttggccccga
     1801 cgtcccagta cttcttcgcg ttgtcgagga ggatgctcac cccgccgcag cctgacccga
     1861 acaccatccc gatccggtcc ggatccgcct cctcggccag gatctggctg tggccgttga
     1921 tgagccccgc atcgcggagt gcctcggcgg tggcggccac cgccatctgg gtcgcgcggt
     1981 cgtggcggcg cacgtccttg gggtccatct cacgggtcgg gtcgaagtcc gggacctcgc
     2041 cgccgatctt gatgtcctgg tcggagaggt ccgcgacggt gacctcccgg atgccggatt
     2101 tgcccgcgac caggttcgcc caggccgtgt cgttgccgat gccgaccggg gagaggacac
     2161 ccagcccggt caccatcgcc cggcgcccgg tatgggtcat gccttcttcc gaggtggttt
     2221 ggaggcgccc ttggagccgc tcgacggctg gggcgacggc agccagccgt catccgcgtc
     2281 atcgagggcg tgcgcgccgg actccgggct gatccgcttg atcaagccgg tcaggacccg
     2341 gccggggccg atctcgacga agtcggtcac gccgatggcg gccatgttgc ggatcgtggc
     2401 gacccattgc acgccgtgga cgaggtgctc gctgagctcg tcgcgcagcc catctgcggt
     2461 ccggatcatg gtg
//
LOCUS       DASYGU010000121         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_638984, whole genome shotgun sequence.
ACCESSION   DASYGU010000121 DASYGU010000000
VERSION     DASYGU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638984"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGO32_03215"
     CDS             complement(<1..387)
                     /locus_tag="VGO32_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199793.1"
                     /translation="MPTFEGAHAETARRAVAAGIIAACLAACAASPSASPSGASSRNP
                     AAFIESQPYAQDIDPADFSDPAPNRYFPLEPGVLTIFEGGGERVEVTVTHETKVILGV
                     TATVITDQVFLDGALAEDTIDWYAADN"
     gene            457..1380
                     /locus_tag="VGO32_03220"
     CDS             457..1380
                     /locus_tag="VGO32_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7199794.1"
                     /translation="MRPFRFGLTAGWVSTGDAWIAMARRAEALGYDILLVPDHLGGQL
                     SPVAALASAAAVTTRLRIGSHVFANDFRHPLVLAREAATLDVLSGGRLEFGIGAGWRV
                     SDYTQLGVPYDPAGRRIDRMEEAVTIIKRLFAGETVTEAGRHYRLRSARLSPRPVQQP
                     RPPILLGGGGPRMLRIAAREADIVGFVPQFSAAGRPMIRQATEAALADKVALVRRAAG
                     DRFDALELNVFIALAGMIGGGTGPLPSAVAAGLAAGAGIVRSPYLLHGTQSRLRDILE
                     RRRDRLGISYYSIPQQAMESMAPLVEDLAGR"
     gene            complement(1408..>2291)
                     /locus_tag="VGO32_03225"
     CDS             complement(1408..>2291)
                     /locus_tag="VGO32_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199795.1"
                     /translation="NGPLDISGQTLGITHAIAGTPMDLVGDPASSLVVTGTGSGIVIP
                     ASLPTLANLTIDNPYGAAIAAPLAIGNLLALTDGVLDAGGWVVVIDPAASVARATGHV
                     SGALQKTVPVGSAVSVFFEIGDATTYAPLTLTFGTVSTSGALTASTTGSEHPSIGSSV
                     IDPTADVNRWWSVSNAGAVFDTVDAVFSWAPPDVDAGADPNAFVVAKWDGSWVLPIST
                     GQMPTSITAMGMTGFSEFAVGEIQPEADGLPDTAGPASRGGMDVGLRGLIVSAILLGL
                     IAIVVVLRPRPETTPEG"
BASE COUNT          325 a          790 c          826 g          350 t
ORIGIN      
        1 gttgtcggcg gcataccagt cgatcgtgtc ctccgccaat gcgccatcga ggaagacctg
       61 gtcggtgatg accgtcgcgg tgacgcccag gatgaccttg gtctcgtgcg tgaccgtgac
      121 ttccacccgc tcgcctccac cttcgaagat ggtcaacacc cccggctcga gtgggaagta
      181 tcgattcggg gcggggtcgg agaagtcggc cgggtcgatg tcctgcgcgt agggctgact
      241 ctcgatgaac gcggcggggt tgcggctcga agcaccgctc ggggaggcgc tgggggacgc
      301 cgcgcaggcg gcgagacagg cggcgatgat cccggcagcg acggcccgtc gtgccgtctc
      361 ggcgtgtgcc ccctcgaagg tcggcatctc cagataccac gatacgcgcc tgcaaacggg
      421 gtgtcagttc ggcacgttgg cgcacactga ccgccgatgc ggcccttccg ctttggcctc
      481 accgccggct gggtttccac cggtgacgcg tggatcgcca tggcccggcg ggcagaggcg
      541 ctcggctacg acatcctgct cgtccctgat cacctgggcg gtcagctgtc cccggtcgcc
      601 gcgctggcct cggcggccgc ggtcaccacc cggctccgca tcggctctca cgtcttcgcc
      661 aacgactttc gccaccccct ggtgctcgcg cgcgaggccg cgaccctgga cgttctgtcc
      721 ggagggcgcc tcgagttcgg gatcggcgcg ggctggcggg tgagcgacta cacccagctt
      781 ggcgttccgt acgacccggc cggaagacgg atcgaccgga tggaggaggc ggtgaccatc
      841 atcaagcggc tcttcgccgg ggagaccgtg accgaggcgg gtcgtcacta ccggctccgc
      901 agcgcccgtc tctccccgcg cccggtgcag caaccccggc ccccgatcct gctgggcggc
      961 ggcgggccgc gcatgctccg catcgccgcc cgcgaggcgg acatcgtggg cttcgtcccg
     1021 cagttcagcg cggcgggcag gccgatgatc cggcaggcga ccgaggcggc cctcgccgac
     1081 aaggtcgccc tcgtgcggcg ggccgccggc gaccggttcg acgcactcga gctgaacgtg
     1141 ttcatcgctc tggccggaat gatcggtggc gggacgggac cgctgccctc ggcggttgcg
     1201 gctgggctgg cggccggagc aggcatcgtc cgctccccgt acctgctcca cggcacgcag
     1261 tcgcggctgc gggacatcct cgagcggcga cgggatcggc tggggatcag ctactactcg
     1321 atcccgcagc aggcgatgga gtcgatggcg ccactggtcg aggacctcgc cggtcggtag
     1381 cgccccgccg agtcgtgggg cggaagccta gccttcgggt gtcgtctccg ggcgcggccg
     1441 gaggaccacc acgatcgcga tcaggcccag caggatggcg gacacgatca ggccccggag
     1501 gcccacgtcc atgccgccgc ggctcgccgg cccggccgtg tccgggagac catccgcttc
     1561 gggctggatc tcgcccacgg cgaactcgct gaagccggtc atccccatcg cggtgatgga
     1621 cgtcggcatc tggccggtcg agatggggag gacccagctg ccgtcccact tggccacgac
     1681 gaacgcgttc ggatcggccc cggcgtcgac gtccggcggc gcccagctga agaccgcgtc
     1741 gaccgtgtcg aagaccgcgc cggcgttcga aaccgaccac catcggttca cgtcagccgt
     1801 tgggtcgatg accgatgagc cgatgctcgg gtgctcgctc cccgtcgtgg aggcggtgag
     1861 agcgccgctc gtgctcacgg tcccgaaggt cagggtcagc ggggcgtagg tcgtggcgtc
     1921 gccgatctcg aagaagaccg acacggccga accgaccgga accgtcttct ggagcgcgcc
     1981 gctgacgtga cccgtggcgc gggccacgga tgccgcggga tcgatgacca cgacccagcc
     2041 tccggcatcc aatacgccat cggtgagggc cagaaggttg ccgatagcaa ggggcgcggc
     2101 aatggccgcc ccgtacggat tgtcgatcgt gaggttcgcc agcgtcggca ggctggcggg
     2161 gatgacgatt ccactgcccg ttccggtgac gaccagcgag gacgcggggt cgccgacgag
     2221 gtccatcggc gttccggcga tggcatgggt gatgcccagg gtctggccgc tgatgtcgag
     2281 cgggccgttc a
//
LOCUS       DASYGU010000122         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_536341, whole genome shotgun sequence.
ACCESSION   DASYGU010000122 DASYGU010000000
VERSION     DASYGU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536341"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VGO32_03230"
     CDS             complement(<1..1428)
                     /locus_tag="VGO32_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEV7199796.1"
                     /translation="MSTRASTDTPLLLAPGTLTPGRARAALPLRREGPDKLTGAAKYA
                     DDLVFPGAWYGATIRSTEPHARLRSIDLDPNFDWSKVVVVTADDIPGDNVVSLIQDDQ
                     PVLVPLGGEIQHQAEPVCLLAAPDRDLLRAAKRHVTLRTERLPAVFDPLLSDREFAHF
                     DLAKGDVEAGLAAADLVIEGEYRVGHQEQLYIENQAMIAVPRPDGGIEVHGSMQCPYY
                     IHGALKRALNLTDGQAVVIQAETGGGFGGKEEYPSIVALHAALLAQRVGKPVRLIYER
                     HEDISATTKRHPAWVRHRTGVMRDGRLVAQDIEVVMDGGAYVTLTPVVLSRGTIHAGG
                     PYECPNVRIRGRAMATNTPPNGAFRGFGAPQTEFAAETQVNRIAERLRISPLALRRRW
                     VYREGGVTPTGQVLRESVAGLEVLEAAAQASEYEGVAGHTKKAREQRREDARTARGIG
                     LALAWHGAGFTGSGEVHLASVASLEL"
     gene            complement(1464..3053)
                     /locus_tag="VGO32_03235"
     CDS             complement(1464..3053)
                     /locus_tag="VGO32_03235"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEV7199797.1"
                     /translation="MTDLVISGGTVVDGSGAPGRPGTLLVTDGRIRLTEPHVAALAAH
                     RTIDASGMVVAPGFIDLHSHSGLWLLHEPRHEAKVRQGVTTEVIGVDGLSYAPLTNPE
                     DLAALVQMNAGLDGRPELALDWDTVASYLGRFDGQVAVNVALLIGNSALRICALGWDD
                     VPAGHMALADMRAMLREGMAEGAFGLSSGLDYPPGSYATTEELADLTMAAARDGGFYH
                     THVRYELGDRYLDPFREALAIGEMAHGPVHLTHFYHRQNYPGGYEPMLVLVDDARSAG
                     RDVTFDTYPYEWASTRLLIQLPQWVQSGGPGALKERLADGATRQRLRDEMSHHDPVHG
                     APLTWADIRLGAFVSVANQSWEGRTLEEVGRETDRDPIDVLCDLLLAEDLRLNQVTPG
                     PWSETLPHFVTHPAGMVGTDSTFIGLKPSPRTYGSYPRVLGQFVRDERRLSLEEAVRK
                     MTSAPAARLGLRDRGLLRDGFVADVVIFDPERVRALATYDEPRQYSEGIEHVLVNGVA
                     VVDGGRHTGALPGRALRHGRD"
     gene            complement(3050..4033)
                     /locus_tag="VGO32_03240"
     CDS             complement(3050..4033)
                     /locus_tag="VGO32_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV7199798.1"
                     /translation="MIDEALAAVRAWRADDRETALATVVRVNGSAPRGVGAKLAVAAG
                     GEMAGSVSGGCVEGDVVEHALAVLASGDPVLRRYGISDEQGMDVGLMCGGTIEVLVEP
                     VEGKRAGLVELFTALVESGRPAAREIAVAGPGPLGTDRVLDGQAAVDAADTAERVHDR
                     ILPAPRVWIVGAGHVAEHVAAYAARAGFLPLVVDPRRLFADQPRFDGMTVIPDWPDRA
                     FAEGDLRADDAVVVLSHDPKIDEPALLAALGSNIAYVGAIGSRRAQVDRRARLRAAGL
                     AEDRLARLHAPIGLDLGGREPPEIGLAIVAELVANRHGREGGPMRDTARSE"
     gene            4052..4624
                     /locus_tag="VGO32_03245"
     CDS             4052..4624
                     /locus_tag="VGO32_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV7199799.1"
                     /translation="MTSRSPVAAVILAAGLGRRFGSPKVVAQLGGQTLLLRVSRLARE
                     AGLNPIIAVVPRGAETPSRVDAVVNTDPARGLSSSLRLGIAAVPANRAALVLLVDQPT
                     MPRETISAVLAARGRRPLVVATADDRYGPPVLIEPEAFDLVSDLSGDVGLRDLLVSRP
                     DLVESVEVPAHAPDVDTAADLERLEDRLGP"
     gene            4621..>5619
                     /gene="ruvB"
                     /locus_tag="VGO32_03250"
     CDS             4621..>5619
                     /gene="ruvB"
                     /locus_tag="VGO32_03250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV7199800.1"
                     /translation="MTIERVGSAEVLEDERSLEATVRPRRLDEFAGQERIKENLAILI
                     DAARQRSEPVDHILLYGPPGLGKTTLATIVAAEVGAQLRTTSGPAIERAGDLAAILTG
                     LQRGDVLFIDEIHRLSHVVEEVLYPAMEDFVLDIVLGKGPGARTVRLQIEPITIIGAT
                     TRVGSITGPLRDRFGVTHRLDYYTDADLVTILDRSAGILEIPLDDDAAVLIARRSRGT
                     PRIANRLLKRVRDYAQVRGDEDGKATAAASEAALGLLDIDERGLDALDRQLLTAIAEH
                     HGGGPVGLQTMAATISEPVETIEDVVEPYLIQAGLLARTPRGRQLTPSAWTHLGLTP"
BASE COUNT          791 a         2003 c         2016 g          809 t
ORIGIN      
        1 gagctcgagc gaagccacgc tggcgaggtg gacctctccg gagccggtga acccggcccc
       61 gtgccaggcg agcgccagac cgatgccgcg ggcggtccgg gcgtcctcgc gccgttgctc
      121 gcgcgccttt ttggtatgtc ctgccacgcc ttcgtactcg ctcgcctggg cggcggcctc
      181 gaggacctcc agcccggcca cgctctcgcg gagcacctgg ccggtgggcg tgacgccacc
      241 ctcgcggtac acccaccgcc gacggagggc cagtggggag atcctgagtc gctcggcgat
      301 gcggttgacc tgggtctcgg ccgcgaactc ggtctgcggg gcaccgaatc cgcggaaggc
      361 cccgttgggc ggggtgttgg tggccatcgc ccgaccacgg atccggacgt tcgggcattc
      421 atacgggccg ccggcgtgga tcgtcccgcg actgagcacg accggggtca gggtgacgta
      481 ggccccgccg tccatcacga cctcgatgtc ctgggccacc agccggccgt cgcgcatcac
      541 cccggtccgg tgccggaccc acgccggatg gcgcttcgtc gttgcgctga tgtcttcgtg
      601 tcgctcgtag atcaggcgca ccggtttgcc gactcgttgg gccaggaggg cggcatggag
      661 ggcgacgatc gaggggtatt cctccttgcc cccgaatcca ccgccggtct cggcctggat
      721 gacgacagcc tgtccatcgg tcaggttcag agcccgcttg agcgcgccat ggatgtaata
      781 cgggcactgc atcgagccgt ggacctcgat cccgccgtcg gggcgcggga ccgcgatcat
      841 ggcctggttc tcgatgtaca gctgctcctg gtggccgacc cggtactcgc cctcgatcac
      901 gaggtcggcg gccgccagcc cggcctccac gtcgcccttg gccaggtcga aatgggcgaa
      961 ctcgcggtcc gagaggaggg gatcgaagac ggccggcagc cgctcggtcc ggagcgtgac
     1021 gtgccgcttc gccgcgcgga gcaggtcgcg gtccggcgcg gcgagcaggc agaccggttc
     1081 ggcctggtgc tggatctctc cgccgagcgg gacgagcacc ggctggtcgt cctggatgag
     1141 gctcaccacg ttgtcgcccg gaatgtcgtc ggcggtgacc acgaccacct tggaccagtc
     1201 gaagttcggg tcgagatcga tgctccgcag gcgggcatgc ggctccgtcg agcggatggt
     1261 ggcgccgtac caggcgccgg gaaagacgag gtcgtcggcg tattttgcgg cgccggtcag
     1321 cttgtccggt ccctcacgcc ggagggggag cgcggcgcgt gcccggcctg gcgtcagcgt
     1381 gcccggtgcg agcaggagcg gtgtgtccgt cgaggcgcgg gtgctcacga cggaatggta
     1441 tccagtcgcg cccctgtcgc gcgctagtcg cgcccgtggc ggagggcgcg tcccggcagc
     1501 gcgccggtat gccggccgcc gtccacgacg gccaccccgt tgaccagcac gtgttcgatc
     1561 ccctccgagt attggcgagg ctcgtcgtag gtggcgagcg cccgtacgcg ttcggggtcg
     1621 aagatcacga cgtcggccac gaagccgtca cgcaggaggc cacggtcgcg gagcccgagt
     1681 cgggcggccg gggcggaggt catcttgcgc accgcctcct cgagcgagag ccggcgctcg
     1741 tcgcggacga actggcccag cacgcgcggg tacgagccat acgtgcgcgg cgagggcttc
     1801 agaccgatga acgtggagtc ggtgcccacc atccccgccg ggtgggtaac gaagtggggg
     1861 agtgtctcgg accacggtcc gggggtgacc tggttgaggc gcagatcctc ggccagcagg
     1921 aggtcgcaga ggacgtcgat cgggtcgcga tcggtctcgc gtccgacctc ctccagcgtc
     1981 cgaccctccc acgactggtt cgccaccgat acgaacgcgc caagccggat gtcggcccac
     2041 gtgagcggcg cgccgtggac cgggtcgtgg tgggacatct cgtcgcggag gcgctgccgc
     2101 gtggccccgt ccgcgagccg ctccttcagc gcccccggac cgccggactg gacccattgc
     2161 gggagctgga tgaggagccg ggtcgaggcc cactcgtagg gatacgtgtc gaacgtcacg
     2221 tcgcgcccgg ccgaccgcgc gtcgtcgacc aggaccagca tcggctcgta cccgccaggg
     2281 tagttctggc ggtggtagaa gtgggtgagg tggactgggc catgcgccat ctcgccgatt
     2341 gccagcgcct ctcggaacgg gtccaggtac cggtcgccga gctcgtagcg aacgtgggtg
     2401 tggtagaagc cgccgtcccg ggcggccgcc atggtgaggt ccgccagctc ttcggtggtc
     2461 gcgtacgagc cgggcgggta gtcaagcccg cttgacagcc cgaacgcgcc ctcggccatg
     2521 ccctcgcgga gcatggcccg catgtcggcc agggccatgt ggccggcggg cacgtcgtcc
     2581 cagcccaggg cgcagattcg gagcgccgag tttccgatca gcagggcgac gttcaccgcg
     2641 acttgtccat cgaagcggcc gaggtatgac gcgacggtgt cccagtccag cgcgagctcg
     2701 ggccggccgt ccagcccggc attcatctgg accagggccg cgagatcctc cgggttggtc
     2761 agcggcgcgt aggacagccc gtccacgccg atgacctcgg tggtgacgcc ctggcggacc
     2821 ttggcctcgt ggcgcggctc gtggaggagc cagaggcccg agtgagagtg gagatcgatg
     2881 aatccgggcg ccaccaccat cccgctcgcg tcgatcgtcc ggtgcgccgc cagcgccgcg
     2941 acgtgcggct cggtgaggcg aatccgaccg tcggtgacga ggagggtccc cggtcgaccg
     3001 ggggcaccgc tgccgtcgac cacggtgccg ccgctgatga cgaggtcggt cattcgctcc
     3061 gggccgtgtc gcgcatcggt ccgccctctc gcccatggcg gttggccacc agctcggcca
     3121 cgatcgccag gccgatctcg ggtggctcac gaccgccgag atccaggccg atcggggcgt
     3181 ggaggcgggc caggcggtcc tcggccaggc cggcggcccg cagccgcgcc cggcggtcaa
     3241 cctgcgcccg tcggctgccg atggccccaa cgtaggcgat gttgctgccc agcgcggcga
     3301 ggagggctgg ctcatcaatc ttcgggtcat gggacagcac gacgaccgcg tcgtcggcac
     3361 gcaggtcgcc ctccgcgaac gcccggtcgg gccagtcggg gatcacggtc atgccgtcga
     3421 agcgcggttg gtccgcgaac aggcggcgcg ggtccaccac cagcggcagg aaccccgctc
     3481 gcgcggcgta ggcggccacg tgctcggcga cgtggcccgc gcccacgatc cagacccggg
     3541 gcgcgggcag gatccgatcg tgcacccgct ccgcggtgtc cgccgcgtcc accgccgcct
     3601 ggccgtccag gacgcggtcg gtccccagcg gcccaggccc ggcgaccgcg atctctcgcg
     3661 ccgccggccg cccggactcc accaacgcgg taaacagctc gaccaggccc gctcgcttgc
     3721 cctcgaccgg ttcaacgagg acctcgatcg tgcccccgca catgaggccg acgtccatgc
     3781 cctgctcatc gctgatcccg tagcggcgaa gcaccgggtc accgctggcc aggaccgcga
     3841 gcgcgtgctc gacgacgtcg ccctcgacgc agccgccgct caccgagccg gccatctcac
     3901 cgcccgcggc cacggccagc ttggcgccga ccccgcgcgg cgcggagcca ttgacccgga
     3961 ccacggtcgc gagcgccgtc tcgcggtcgt ctgctcgcca ggcgcgaacc gcggcgagcg
     4021 cctcgtcgat catgggtcca gtatgcgacc gatgacgagc cgatcaccgg tggccgccgt
     4081 gatcctcgcg gcgggcctcg gcagacgatt cggaagcccg aaggtcgttg cccagctggg
     4141 tggccagacg ttgctcctgc gcgtgtcgcg gctggcccgc gaggccgggc tgaacccgat
     4201 catcgcggtc gtcccccgag gagcggagac gccgtcgcgg gtcgatgcgg tggtcaacac
     4261 cgacccggca cggggtctca gctcttccct ccggctgggg atcgcggcgg tgcccgccaa
     4321 tcgggccgcg ctcgtcctgc tcgtggacca gcccaccatg ccccgagaga cgatcagcgc
     4381 cgtgctcgcg gcgcgcggac gccgaccgct ggtggtggcc accgccgacg atcggtacgg
     4441 gccgccggtg ctcatcgaac ccgaggcgtt cgatctcgta tctgatctga gcggcgatgt
     4501 cggtctccga gacctgctcg tcagccgccc ggacctcgtg gagtcggtgg aggtcccggc
     4561 ccatgccccc gatgtcgaca ccgcggccga ccttgagcgg ctggaggata gactcgggcc
     4621 gtgaccatcg aacgggtcgg cagtgccgag gtgctcgagg acgaacgttc gctcgaggcc
     4681 accgtccggc cgcgccgatt ggacgaattc gccggccagg agcggatcaa ggagaacctc
     4741 gccatcctca tcgacgcggc ccgacaacga tcggagccag tcgaccacat cctgctctat
     4801 ggcccacccg gcctcggaaa gaccacgctg gcgaccatcg tcgcggccga ggtcggcgct
     4861 cagctccgga cgacctccgg gccggccata gaacgggccg gcgacctggc ggcgatcctc
     4921 accggcctcc agcgcggcga cgtcctgttc atcgacgaga tccatcgcct gtcgcacgtc
     4981 gtcgaggagg tcctctaccc ggcgatggag gacttcgtgc tcgacatcgt gctgggcaag
     5041 gggccgggcg cgcggacggt gcggctccag atcgagccga tcacgatcat cggggccacg
     5101 acccgggtgg ggagcatcac tggtccgctc cgtgaccggt tcggggtcac ccatcggctc
     5161 gactactaca ccgatgcgga cctggtcacg atcctggacc gcagcgccgg catcctcgag
     5221 atcccgctgg acgatgacgc cgccgtgctc attgcccgcc ggagccgcgg gacgccgcgg
     5281 atcgcgaatc ggctcctgaa gcgagtgcgc gactacgccc aggtgagggg tgacgaggac
     5341 ggcaaggcca ccgccgccgc gtccgaagcc gccctgggcc tgctcgacat cgacgagcgg
     5401 gggctggatg ccctcgaccg ccagctgctc accgccatcg ccgagcacca tggcggggga
     5461 ccggtgggcc tccagacgat ggcggccacg atcagcgagc cggtggagac gatcgaggac
     5521 gtggtcgagc cgtatctcat ccaggccggg ctgcttgccc gcaccccacg cggccggcag
     5581 ctgaccccgt ccgcctggac ccacctggga ctgacgccg
//
LOCUS       DASYGU010000123         6042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_227511, whole genome shotgun sequence.
ACCESSION   DASYGU010000123 DASYGU010000000
VERSION     DASYGU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227511"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGO32_03255"
     CDS             complement(<1..204)
                     /locus_tag="VGO32_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199801.1"
                     /translation="MLPGFIFVSSVGVAILVLLASWMQPRQFVLTSAIVAVLVMLAAA
                     QGVMLWQRVTAANRAGARRAGLDS"
     gene            336..866
                     /locus_tag="VGO32_03260"
     CDS             336..866
                     /locus_tag="VGO32_03260"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV7199802.1"
                     /translation="MTATSTDDLRKLIREVPDFPKPGILFYDITTLLKDPSAFRQVID
                     QMVEQVRGTEIDVVVGMESRGFIFSAPIAYQLNAGFVPVRKLGKLPAETIEVEYALEY
                     GTATLEIHRDAIQEGQRVLIVDDLLATGGTVKGTIELIHRLGGQVAGLSFMVELSALH
                     GREQLSEFTINALLTY"
     gene            889..1749
                     /locus_tag="VGO32_03265"
     CDS             889..1749
                     /locus_tag="VGO32_03265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7199803.1"
                     /translation="MTTASAIGWRTREVTDRDEVAAFLRTDRLYAAYALGDLAASVRR
                     RAAWGMADDEHGRPRGLAMQHLGLPPQPLFLMGDPGACRAILANEVRPRYAFVQARHD
                     LAPAFNELYRVDLPVALLRMVVDRATFRPHAGGAERLGPADIGDLNRLYQLGLGGGFP
                     ASVLEDGVYYGVRVGGRLIAAAGTHVINEAEGIAVVGNVLTHGDHRGQGHAKAATSGV
                     TADLLTRVPDVALNVYADNDPAVTAYSRLGYREYCRLGEWVAYRRAGGWDLIRPLRAA
                     LRWSWPRAGR"
     gene            1772..3025
                     /locus_tag="VGO32_03270"
     CDS             1772..3025
                     /locus_tag="VGO32_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV7199804.1"
                     /translation="MSDIRDPGLADDGARRIEWADRQMPVLAGIRDRLSVSRPLDGWR
                     VSACLHVTAETANLARTLSAAGADVVLCASNPLSTQDDVAAALVSDHGIEVFAMRGED
                     RDTYYGHINAALDHRPQLTMDDGADLVGELHTHRSDLLTEVRGGTEETTTGVIRLRAM
                     AADGALRYPILAVNEALTKHLFDNRYGTGQSAVDGILRATNVLLAGKTAVVGGYGWVG
                     RGIASRIHGMGANVVIVEIDPIRALEAAMDGFRVMTSLDAAEVGDLFITATGSLHVFG
                     GEHFKIMRDGAILANAGHFNDEFDIPALEALAGEQREVRPSTREYRLVDGRRLYLLAD
                     GRLVNLAAAEGHPSLVMDLSFANQALGLEWLSENHGELVPDVYDIPIEIDREVARLKL
                     ASMGIAIDRPTTEQEAYAASWQQGT"
     gene            complement(3032..3571)
                     /locus_tag="VGO32_03275"
     CDS             complement(3032..3571)
                     /locus_tag="VGO32_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199805.1"
                     /translation="MRRPSLLLVLTAVIGLVLAACASPAGSPGASGGGEPSSAAGSEA
                     PAGSDDGSGGGGGGGGSASGSLVYDISGDYSASGELPYQSILGGYLDLQGVTYLPFAE
                     TESSDEVAFITLSDEQNIFAFGNGEFSVPAADCDWNVTRNDATGAAGSFECTDAMGIH
                     VGEQTFVTVDISGSFDTSS"
     gene            3678..4844
                     /gene="metK"
                     /locus_tag="VGO32_03280"
     CDS             3678..4844
                     /gene="metK"
                     /locus_tag="VGO32_03280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV7199806.1"
                     /translation="MRLFTSESVTEGHPDKVCDAIADAILDAVLAVDPAAHAAIEVAA
                     TTGTVVVLGELTTEHPVDVDDLVRRTVREIGYDDPALGFDADSVTVTSLIKGQSPDIA
                     LGVDEHAAELGAGDQGMMFGYATDETTELMPMPIQLAHELCRRLAEVRRAGTVPYLRP
                     DGKSQVTVAYGHDRLPRSVHTVLISTQHADTIEAADIANAVRREVVDAVIPADLRDGE
                     MRLLVNPTGRFVLGGPAGDSGLSGRKIIVDTYGGMARHGGGSFSGKDPTKVDRSGAYA
                     ARWIAKNVVAAGLATRCEIQVAYAIGIARPLALNVDTFGTGVVPDARIRAAIDAVVDL
                     RPGAIIERLALRRPIYRPTAAYGHFGRRDLDLSWERTDLADELLQAARPAGTAA"
     gene            4841..5458
                     /locus_tag="VGO32_03285"
     CDS             4841..5458
                     /locus_tag="VGO32_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199807.1"
                     /translation="MSHRSVPVRWGPPAPGPAGRFRAWLRSLAGSKPDTVQRLADLPL
                     ALRQAPLAAPYLEAEHAGDERRAGAAAMAAAEAGIASGAWWAADLWAHRALWHFERAD
                     MALPSTRAARRIGDLRVAGGDPASARRYYAEAISEARDLGAEREEGLAAYGMGRAELE
                     LGNVTAARKLAHIAVGLLERCDAPEDELAAARELQGTERAVGGPG"
     gene            5458..>6042
                     /locus_tag="VGO32_03290"
     CDS             5458..>6042
                     /locus_tag="VGO32_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7199808.1"
                     /translation="MATRQAMQVGIVGAGVMAEAMIAGLLADRAVRAADLVASHPRRD
                     RRASLTAEHGIRVVARNREAAEGADIVVLAVKPQMLRAVMRELAPTLTDGQVVLSIVA
                     GATLKTLSAGLRHAAVVRAMPNTPAQIRKGIAVWAAAPACSAAQRDRARAVLRALGDE
                     RQVDDEQFVAMATALSGTGPTYLFAVMEALVDAGV"
BASE COUNT          917 a         2136 c         2127 g          862 t
ORIGIN      
        1 cgagtcgagg cccgcgcggc gcgcgccggc ccggttggcc gccgtcaccc gctgccagag
       61 catcacgccc tgcgcggcgg cgagcatgac gagcacggcc acgatcgcac tggtcagcac
      121 gaactggcga ggctgcatcc agctggccag caggacgagg atggccaccc cgaccgagga
      181 cacgaagatg aagcccggca gcatgcgttc catgcgccgc cgcgcccttc ccatcgacat
      241 cggtctgaaa cgtagtgctg aagggcccca cgcacgatgg gccaatggtc cggtcgagat
      301 tgcgagcgcc tcactgcgcc gactagactc ctgccgtgac cgccacctcc accgacgacc
      361 tgcggaagct catccgcgag gtccccgact ttccgaagcc gggcatcctg ttctacgaca
      421 tcaccaccct gctgaaggat ccgtcggcct tccgccaggt catcgaccag atggtcgagc
      481 aggtgcgcgg caccgagatc gacgtggtgg tgggcatgga gtcgcgcgga ttcatcttca
      541 gtgcgccgat tgcctaccag ctgaatgccg gcttcgtccc cgtccgcaag ctcggcaaac
      601 tgccggccga gacgatcgag gtcgagtacg ccctcgagta cggcaccgcc accctcgaga
      661 tccatcgtga tgcgatccag gagggtcagc gcgtgctcat cgtcgacgat ctgctggcca
      721 ccggcgggac ggtgaaaggg acgatcgagc tcatccaccg actgggcggc caggtggccg
      781 ggctctcgtt catggtcgag ctcagcgccc tccacggccg cgagcagctg tccgagttca
      841 cgatcaacgc gctcctcacc tactgaccga tccgcgcctg ggcgaccgat gacgacagcc
      901 tccgcgatcg gctggcggac gagagaggtc accgaccgcg acgaggtcgc ggcattcctc
      961 cgcaccgaca ggctgtatgc cgcctacgcg ctcggcgacc tggctgccag cgtgcggcgc
     1021 cgtgcggcct ggggcatggc cgatgacgag cacggccggc cccgggggct cgccatgcag
     1081 cacctcggtc tgccccccca gccgttgttc ctgatgggtg accccggcgc ctgccgggcg
     1141 atcctcgcca acgaggtccg gccccgctac gcgttcgtcc aggcccgaca cgacctcgcc
     1201 ccggccttca acgagctgta ccgggtggac ctccccgtcg ccctgctccg gatggtcgta
     1261 gaccgggcga ccttccggcc ccatgcgggc ggcgcggagc ggcttgggcc ggccgacatc
     1321 ggcgacctga accgcctgta ccagcttggc ctgggcgggg gatttccggc gtccgtgctg
     1381 gaagacggcg tctactacgg cgtgcgggtc gggggccggc tgattgccgc tgccgggacg
     1441 catgtcatca acgaagcgga ggggatcgcg gtcgtgggca acgtcctgac ccacggcgac
     1501 catcgtgggc agggccacgc caaggcggcg acctcgggcg tgaccgcgga tctcctgacc
     1561 cgtgtgccgg atgtcgcgct caacgtgtac gccgacaacg acccggcggt cacggcctac
     1621 tcccggctgg ggtaccgtga gtactgccgc ctcggagagt gggtcgcgta ccgccgagcc
     1681 ggtggctggg acctcattcg cccgctgcgc gctgcgctgc gctggagctg gccccgcgcc
     1741 ggccgttgat cagaccgatg atggaggcac cgtgagcgac attcgagacc ccgggctggc
     1801 tgacgacggc gcgcgtcgca tcgaatgggc cgaccgccag atgcccgtgc tggccgggat
     1861 ccgtgatcgg ttgagcgtct cccggccgct cgatggctgg cgggtcagcg cctgcctgca
     1921 cgtgacggcc gagacggcaa acctcgcgcg gaccctgtcc gcggccggcg ctgacgtcgt
     1981 gctctgcgcc tcgaatccgt tgtcgaccca ggacgacgtc gccgccgcac tcgtgtccga
     2041 ccacgggatc gaggtgttcg ccatgcgcgg cgaggatcgg gacacgtact acggccacat
     2101 caacgccgcc ctggaccacc gcccgcagct gaccatggac gacggcgctg acctggtcgg
     2161 ggagctgcat acccatcggt ccgacctgct gaccgaggtg cggggtggga cggaggagac
     2221 gacgacgggt gtcatccggc tgcgggcgat ggccgccgac ggggccctcc gctacccgat
     2281 cctggccgtg aacgaggccc tgaccaagca cctgttcgac aaccgctacg gcaccggaca
     2341 gagcgcggtg gacggcatcc tgcgcgcgac gaacgtgctg cttgcgggca agaccgcggt
     2401 tgtgggcggc tacggctggg tcggccgcgg gatcgcgtcc cggatccacg ggatgggggc
     2461 caacgtggtg atcgtggaga tcgatccgat ccgcgcgctc gaggcggcga tggacggctt
     2521 ccgggttatg accagccttg acgccgccga agtgggggac ctgttcatca ccgccaccgg
     2581 ttcacttcac gtgttcggcg gtgagcactt caagatcatg cgcgacgggg cgatcctggc
     2641 caacgcgggc cacttcaacg acgagttcga catccccgcg ctcgaggcgc tggccggcga
     2701 gcagcgcgag gttcgaccgt cgacccgcga gtaccggctg gtcgacgggc gccggctcta
     2761 cctgctggcc gatggccggc tggtcaacct ggcggcggcc gagggccacc ccagcctggt
     2821 gatggacctc tccttcgcca accaggccct gggcctggag tggctgagcg agaaccacgg
     2881 cgagctcgtg ccggacgtgt acgacatccc gatcgagatc gaccgcgagg tggcgcggct
     2941 caagctggcc agcatgggaa tcgccatcga tcgccccacc accgagcagg aggcctacgc
     3001 ggcctcctgg cagcagggaa cctagccggc gctagctcga ggtatcgaac gaaccagaga
     3061 tatcgaccgt cacgaacgtc tgctcgccga cgtggatgcc catcgcatcg gtgcactcga
     3121 acgatccggc ggcaccagtg gcgtcgttgc gggtgacgtt ccagtcgcaa tcagcggccg
     3181 ggacggagaa ttcaccattg ccgaaggcga atatgttctg ctcgtcgctg agggtgataa
     3241 atgcgacctc gtcgctgctc tccgtctccg cgaacggcag gtaggtcacg ccctgcaggt
     3301 cgagataccc gcccaagatc gactggtaag gcaattcccc gctagcggag tagtcgccgg
     3361 agatgtcata gacgagagac ccgctggccg atccaccgcc gccgcctccg ccaccactcc
     3421 catcgtcgct cccggccgga gcctccgatc cagcggcact ggagggctca ccgcccccgg
     3481 aggcgcctgg gcttccggcg ggcgaggcgc aggccgcgag gaccaggccg atgacggcgg
     3541 tcaggaccag caacagggat gggcggcgca tgggcgttct cctgtgaaat gagggagcgg
     3601 gagcatacca gccgcccacg gtgggtccgc cattgcatcg gtctggcgct acactcggac
     3661 ccttgctcga cgccgacatg cgcctgttca cctcggaatc ggtgaccgaa ggccaccccg
     3721 acaaggtctg cgacgcgatc gccgacgcga ttctcgacgc ggtgctggcc gtggatccgg
     3781 ccgcccacgc ggcgatcgag gtggcggcca cgaccggcac cgtggtcgtg ctgggtgagc
     3841 tcacgaccga gcacccggtg gatgtcgatg acctcgttcg cagaaccgtc cgcgagatcg
     3901 ggtatgacga tccggcgctc ggcttcgacg ccgactcggt aaccgttaca tccctgatca
     3961 agggccagtc acccgatatc gcgctgggcg tggacgagca tgccgccgag ctgggagccg
     4021 gcgaccaggg aatgatgttc ggctacgcga ccgatgagac caccgagctc atgcccatgc
     4081 cgatccagct tgcccatgag ctgtgtcgcc ggctggccga ggtccgacgg gccgggaccg
     4141 tgccgtacct ccgacccgac ggcaagagcc aggtcacggt ggcgtatggc cacgaccgac
     4201 tgccgcggag cgtgcacacc gtgctcatca gcacccagca cgccgacacc atcgaggcgg
     4261 ccgatatcgc gaacgcggtc cggcgcgagg tcgtggatgc ggtcatcccg gccgacctgc
     4321 gcgacgggga gatgcgtctg ctggtgaacc cgaccggccg gttcgtgctg ggcggcccgg
     4381 ctggagactc ggggctcagc ggccgaaaga tcatcgtgga cacctacggc ggcatggccc
     4441 gccatggcgg aggctcgttc tcgggcaagg acccgaccaa ggtggaccgc agcggggcgt
     4501 acgccgcgcg ctggatcgcc aagaacgtgg tcgccgccgg gctggccacg cgctgcgaga
     4561 tccaggtcgc gtacgcgatc gggatcgccc ggccgctggc tctcaacgtc gacaccttcg
     4621 gcaccggcgt cgtgccggac gccaggatcc gggcggccat cgatgcggtc gtggacctgc
     4681 ggccgggggc gatcatcgag cgcctcgccc tgcgccgccc gatctaccgc ccgacggcgg
     4741 cctacggcca ctttggccgc cgcgacctcg atctcagctg ggagcgcacc gatctcgccg
     4801 acgagctgct ccaggcggcc cgtccggcgg gcaccgccgc gtgagccacc gttcggtccc
     4861 cgtccgatgg gggccgccag cgcccggacc ggcgggccgc ttccgtgcct ggctgcgaag
     4921 ccttgccgga tccaagcccg ataccgttca gcgtctcgcg gaccttccgc tcgccctgcg
     4981 tcaggcccca ctcgcggcgc cctacctcga ggccgagcac gcgggcgacg aacggcgcgc
     5041 gggcgcggca gccatggccg ccgccgaggc cgggatcgcg tccggcgcat ggtgggcggc
     5101 cgacctgtgg gctcatcgcg cactgtggca cttcgagcgg gcggacatgg cgctcccgtc
     5161 gacccgagcg gcgcggcgga tcggtgacct gcgcgtcgcc ggcggcgatc cggcgagcgc
     5221 ccggcgctac tatgccgagg cgatcagcga ggcccgggac ctcggcgccg agcgcgagga
     5281 gggtcttgcc gcgtatggaa tgggtcgtgc tgaactcgag ctgggcaacg tgaccgccgc
     5341 ccgcaagctg gcccacatcg ccgtcgggct cctggagcgc tgcgacgcgc ccgaggacga
     5401 gctcgccgct gcgcgcgagc tccagggcac cgagcgcgcg gtcggaggtc cgggctgatg
     5461 gccacgcggc aggcgatgca ggtcgggatc gtgggcgccg gggtgatggc cgaggccatg
     5521 atcgcgggcc tgctggcgga tcgggcggtc cgcgccgccg acctggtcgc ctcgcatccc
     5581 cggcgcgacc gccgagcgtc actcaccgcc gagcacggca tccgggtcgt ggcccgcaac
     5641 cgtgaggccg ccgagggcgc ggacatcgtg gtcctggccg tcaagccgca gatgctgcgc
     5701 gcggtgatgc gtgagctcgc ccccaccctg accgacggcc aagtcgtgct gtccatcgtg
     5761 gccggcgcaa ccctgaagac gctcagcgcc ggcctccgcc acgcggcggt ggtgcgggcc
     5821 atgcccaaca ccccggccca gatccggaag gggatcgcgg tatgggccgc ggcccccgcc
     5881 tgcagcgcgg cccagcgtga tcgggcgcgg gccgtccttc gcgcgcttgg cgatgagcgg
     5941 caggtggacg acgagcagtt cgtggccatg gccaccgccc tgtcgggcac cgggccgacc
     6001 tacctgttcg cggtgatgga ggcgctggtc gacgccggcg tc
//
LOCUS       DASYGU010000124         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_278527, whole genome shotgun sequence.
ACCESSION   DASYGU010000124 DASYGU010000000
VERSION     DASYGU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278527"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..973
                     /locus_tag="VGO32_03295"
     CDS             86..973
                     /locus_tag="VGO32_03295"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEV7199809.1"
                     /translation="MTASTSPEAAPLPEIDAPQVIEVIVGFIRSQLEQTGFERLVVGL
                     SGGVDSATVAYLAARAVPAENLLAVRMPYRTSDPSSETDALAVIEALGCDAQRVDLTA
                     AADPLLTLVGHGDEPAIRVRRGNVLARLRMIVLYDRSAAFDALVCGTSNKTEALLGYG
                     TLHGDMAAAIQPIGDLYKSQLRAVATVLGVPPQIVAKPPSADLWPGQTDEDELGATYD
                     DLDRILFAVVDRRWTVDRCVRAGMDGDFVRWVVERVARNEFKRQMPPAAKVSLRTPGI
                     DHLYPRRRPGSRRSTPPAD"
     gene            982..1854
                     /gene="rsmI"
                     /locus_tag="VGO32_03300"
     CDS             982..1854
                     /gene="rsmI"
                     /locus_tag="VGO32_03300"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV7199810.1"
                     /translation="MAGRLVVVGTPIGNLGDLSPRAADALREADLVVAEDTRVAAKLL
                     RFIGARRPTQSFTEHNAAERLPGLLDRLAAGDTLALTTDAGMPAVSDPGAELVAAARA
                     AGHEVTVVPGPSAVTAALALAGVSGAAGTGFLFGGFLPARPASARHAEMERLVAAAAS
                     VGLPLILFEAPHRVRRLLEELADLVDQHPGLQVAAGRELTKLHEEVVAGTPSEVAAAL
                     PAPRGEFTVVASGFAPAEGAAPAVDAHVLAAAARAAGLSDRSVVEILRAAGIGRREAY
                     ALAQSSHSGSASRK"
     gene            1851..>2470
                     /locus_tag="VGO32_03305"
     CDS             1851..>2470
                     /locus_tag="VGO32_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199811.1"
                     /translation="MTQALTTDVRLPDGYTARPVDPDPDARAIYELCAEAAVAEYGTP
                     DVTPQLVRESWNTPGFVPERDTRLVLDVASQPAALVEYYENGPAYVAPFMYVRVRPHL
                     LGAGLVEALVAWAEQRSQQAVERAAPDLRVALHTSVAAANPETIGILERMGWAFERVF
                     WTMEIDLGDEPPEPVVLPDGILIRSAEAGQDEPAIHQLEADAFSGH"
BASE COUNT          330 a          902 c          871 g          367 t
ORIGIN      
        1 tggccgacga gcggctcgag cttacccggc gcgagctgga ccggatcatc gccgagcgtg
       61 ccgggcttga cccggccgag gaccggtgac cgcttccacc agccccgagg cggccccgct
      121 gccggagatc gacgcaccgc aggtgatcga ggtcatcgtc ggcttcatcc gcagccagct
      181 ggagcagacc ggcttcgagc ggctggtcgt gggcctctcg ggcggcgtgg acagtgcgac
      241 ggtcgcgtac ctcgccgccc gggcggtgcc tgctgagaat ctgctggcgg tccggatgcc
      301 gtaccggacc tcggacccgt cctccgagac cgatgcgctc gccgtcatcg aagcgctcgg
      361 gtgtgacgcc cagcgcgtgg atctcaccgc cgcggccgac ccgctgctca ccctcgtcgg
      421 ccacggggac gaacccgcga tccgtgtccg ccgaggcaac gtcctggccc ggctgcggat
      481 gatcgtgctc tacgaccgtt ccgcggcgtt cgacgccctc gtatgcggga cctcgaacaa
      541 gaccgaggcg ctgcttggct acgggaccct ccatggcgac atggcggcgg ccatccagcc
      601 tatcggcgac ctgtacaaga gccagctccg ggccgtcgcg accgtccttg gcgtgccgcc
      661 tcagatcgtg gccaagccac catcggccga cctgtggccc gggcagaccg acgaggacga
      721 gctgggagcc acgtatgacg acctcgatcg gatcctgttc gcggtcgtcg accggcgctg
      781 gacggtggac cgatgcgtgc gggcgggcat ggacggcgac ttcgtgcgct gggtggtcga
      841 gcgggtggcg cgcaacgagt tcaagcgcca gatgccgccc gcggccaaag tgagcctgcg
      901 cacgccgggc atcgaccacc tgtatccccg ccgccgcccc ggctcccggc gctcgacccc
      961 gcccgccgac tgaccggacc gatggccggc cggctcgtgg tagtgggcac tccgatcggg
     1021 aatctcggcg acctgtcgcc ccgggcggcg gacgcgctgc gcgaggccga tctcgtcgtc
     1081 gccgaagaca cgcgcgttgc ggcgaagctg ctccgcttca tcggcgcccg acggccgacc
     1141 caatcgttca ccgagcacaa cgccgctgag cggctgcccg gcctcctgga ccgcctcgcg
     1201 gccggagaca cgctggcgct gaccaccgac gcgggaatgc ccgccgtctc cgatccgggg
     1261 gccgaactgg tggccgctgc ccgtgccgcc ggacacgagg tcaccgtcgt gcccggcccc
     1321 tcggccgtga cggccgcgct cgcccttgcc ggcgtctcgg gcgcggcggg caccgggttc
     1381 ctgttcggtg gcttcctgcc ggcgcggccg gcgtccgccc gccatgccga gatggaacga
     1441 ctggtcgccg cggcggcgag cgtgggcctc cctctgatcc tgttcgaggc tccgcaccgg
     1501 gtgcgccgcc tgctcgagga gctcgcggac ctcgtcgatc agcaccccgg tctccaggtc
     1561 gcggccgggc gagagctgac caagctccac gaggaggtgg tggccggcac cccgtcggag
     1621 gtcgcggccg ctctgcccgc gccgcgcggg gagttcacgg tcgtcgcgtc ggggtttgcg
     1681 cctgctgaag gtgcggcgcc agccgtcgat gctcatgtcc tggccgccgc agcccgggct
     1741 gccggacttt cggaccgatc ggtggtcgag atcctccgcg cggccggcat cggtcgccgt
     1801 gaagcctatg ctctggccca gtcgtcgcat tcggggagtg catccaggaa atgacccagg
     1861 ccttgaccac cgatgtcagg ctgcccgatg gctacaccgc ccggcctgtg gaccccgatc
     1921 ccgacgcccg cgccatctac gagctgtgcg ccgaggccgc cgtggccgag tacgggacgc
     1981 ccgatgtcac gcctcagctg gtccgagaga gctggaatac ccccggcttc gtcccagagc
     2041 gggacacccg cctcgtcctg gacgtcgcca gccagccggc ggcgctggtg gagtactacg
     2101 agaacggccc tgcctacgtc gcaccgttca tgtatgtccg ggtccgcccg catctgctcg
     2161 gtgcgggcct cgtggaagcg ctcgtggcct gggccgagca gcgcagccag caagccgtgg
     2221 agcgggcggc gcctgatctt cgcgtcgcgc tccacaccag cgtggcggcg gcgaatccgg
     2281 agacgatcgg gatcctcgag cggatggggt gggcgttcga acgcgtcttc tggacgatgg
     2341 agatcgatct cggtgacgag ccacccgagc ccgtggtgct ccccgacggg atcctgatcc
     2401 gcagcgcaga agcgggacag gacgagccgg ccattcatca gctcgaggcc gatgccttct
     2461 cgggccattt
//
LOCUS       DASYGU010000125         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_999127, whole genome shotgun sequence.
ACCESSION   DASYGU010000125 DASYGU010000000
VERSION     DASYGU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999127"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VGO32_03310"
     CDS             <1..1083
                     /locus_tag="VGO32_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV7199812.1"
                     /translation="NILYHKPGLELHERLPRYFPNAGDEEIGLFLSNSGAEAIEAAVK
                     LAKIATHRPAIVAFRGGFHGRTHGTMSLTSSAIKNRGHYEPLLASVYFAPYPYALRTP
                     TGRTEADALAWTLSQLDELFATLVFPEDVAAFLVEPVLGEGGYVVPPDGFLPALREVA
                     DSHGILLIADEVQSGFGRTGRFFASEWTEARPDIVVMAKGIASGMPLSGILASRELLA
                     KFPPGGHGGTYGGNAVSCAAAVATLDVFEAEGLTARAETLGHRLLNGLREAAAGNPYV
                     AEIRGRGLMVGIEFADPETLTPRPDLAKAAITDALIHRLLLLSCGTWGQVVRVIPPLV
                     TTEAEIDQAIAIISAALTVAQESAAA"
     gene            1328..1975
                     /locus_tag="VGO32_03315"
     CDS             1328..1975
                     /locus_tag="VGO32_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7199813.1"
                     /translation="MANREQRLTRAMWLIRRDLDDAGRELRDARRRSGLTQQVVASAI
                     GVSHQTVLRNERGSSGASIPALARHAAVVGLRVRIRLYPEGEALRDAGQVALIRRFRE
                     EVGDVGLWSFEVPIPQSGDQRAFDAVVQLGGGQVGLEFYTRLADAQAQLRAANLKKRD
                     ANLGRLVIVVQSTRTNRRALAQAGTVLAEFPGRSGRLLAALKGEQMPETDGVILF"
     gene            complement(1972..>2230)
                     /locus_tag="VGO32_03320"
     CDS             complement(1972..>2230)
                     /locus_tag="VGO32_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199814.1"
                     /translation="ISQEFGGVDTFVDSLVRAVIDGGTLGGGATLVALLDRGDLSIHI
                     PRRDGPAWNVVPLLHLHPGEQPTDADWASANAILERLERYR"
BASE COUNT          362 a          776 c          746 g          346 t
ORIGIN      
        1 aacatcctct atcacaagcc cgggctcgag ctccacgaac ggctgccccg ctacttcccg
       61 aacgcaggcg acgaagagat cggcctgttc ctgtcgaact cgggggcaga ggccatcgag
      121 gccgccgtca agctggccaa gatcgcgact catcggccgg cgatcgtcgc gttccggggc
      181 ggattccacg gccggacgca cgggacgatg tcgctcacct cctcggccat caagaaccgc
      241 ggtcattacg agccgctcct ggcatcggtc tatttcgcgc cgtacccgta cgctctccga
      301 accccgaccg gtcggaccga ggcggacgcc ctggcctgga cgctctccca gctggatgag
      361 ctgttcgcca cgctcgtgtt ccccgaggac gtggccgcgt tcctggtgga gcccgtgctc
      421 ggcgagggcg gctacgtcgt cccgcccgac ggcttcctcc ccgccctccg cgaggtggcc
      481 gatagccacg ggatcctgct catcgccgac gaggtgcagt ccggattcgg acgcacgggc
      541 cgcttcttcg caagcgagtg gactgaagcc cgcccggaca tcgtcgtcat ggccaagggc
      601 attgcctcgg gcatgccgct gtcgggcatc ctcgcctcgc gcgagctgct ggccaagttc
      661 ccgcccggtg gccacggcgg gacgtatggc ggcaacgcgg tgtcgtgcgc ggccgcggtg
      721 gcgaccctcg acgtattcga ggccgagggc ctgaccgccc gcgccgagac cttgggccat
      781 cgcctgctga acgggctgcg cgaggccgca gcgggcaacc cgtacgtggc cgaaattcgt
      841 ggccgcggcc ttatggtcgg catcgagttc gcggatcccg agaccctcac ccctcgcccg
      901 gacctggcca aggcggccat caccgacgcg ctcatccatc ggctcctgct cctgtcctgc
      961 gggacgtggg gccaggtcgt gcgggtcatc ccgccgctgg tgaccaccga ggccgagatc
     1021 gaccaggcga tcgcgatcat ctcggccgcg ctgaccgtcg cccaggagtc cgccgccgcc
     1081 tgaccgtagg cgccgcccgt aggcgacaga tcccggcagt gtccacggga gggacacatc
     1141 ggtctcgaat tggccgatcc caacggtcca gcccactgaa tctgtcgccg tcgtcctcaa
     1201 gaaggaccat tcgcgaccaa taggagcgga accgccatgc ctccgacccg atggcgtcgg
     1261 atgcgtccat acggtggacg aactaaggac aaacctccgg gtaagcccgc ccaatcaccc
     1321 ttgaccgatg gcgaatcgcg aacagcggct gacgcgggcg atgtggctga tccggcgcga
     1381 ccttgatgac gcaggacgcg agctgcggga cgcgaggcgg cgctcgggcc tgacccaaca
     1441 ggtcgtcgcg tcagcgatcg gcgtctcgca ccagaccgtt ctcaggaacg aacgaggttc
     1501 tagcggagcc tccattcccg cgttggcgcg ccatgcggcg gtcgtgggtc tgcgcgtgag
     1561 gatccggctg tatcccgagg gagaggcatt gcgggacgcc ggccaggtcg ccttgattcg
     1621 ccgattccga gaggaagtcg gtgacgtggg cctctggtca ttcgaggtcc ccatcccgca
     1681 gtccggcgac caacgcgcat ttgacgcggt agtccagctc ggaggcggac aggtgggact
     1741 ggagttctac acgcgactgg ccgatgccca agcacaactc cgagccgcca acctgaagaa
     1801 gcgcgatgcc aacctgggca ggctcgtcat cgtggtccag tcgacgcgga ccaatcgtcg
     1861 tgccctcgcc caggccggaa cggtcctggc cgaatttccg gggaggagcg ggcgcttgct
     1921 cgccgcgctc aagggagaac agatgcccga gaccgacgga gtgatcctgt tctagcggta
     1981 ccgctccagg cgctcgagga tggcgttggc cgatgcccag tcggcgtcgg tgggctgctc
     2041 cccggggtgc aggtggagga gcggcaccac gttccaggcc gggccgtcgc ggcgcgggat
     2101 gtgaatcgag agatcgccgc ggtcgaggag cgcaacgagc gtcgctccgc cgccgagcgt
     2161 cccgccatcg atcaccgccc gaaccaggct gtcgacgaag gtgtcgaccc caccgaactc
     2221 ctgacttatc
//
LOCUS       DASYGU010000126         8806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1102306, whole genome shotgun sequence.
ACCESSION   DASYGU010000126 DASYGU010000000
VERSION     DASYGU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102306"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /gene="carB"
                     /locus_tag="VGO32_03325"
     CDS             complement(<1..1722)
                     /gene="carB"
                     /locus_tag="VGO32_03325"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV7199815.1"
                     /translation="MDRRRVLIIGSGPIVIGQAAEFDYAGVQACLALRGEGIDTVLVN
                     SNPATVMTDPDVGGTVVIGPLTVPHLERVIAEHRPDALLPTLGGQTALNLAVGLDDAG
                     VLERYGVAVLGTPLTAVRAAEDRGGFRSLVQSIGEPVPESAVVESVEAGLVFVDDLGA
                     PIVVRPAFTLGGGGGGFASGRDEARTAIARGLAASPIGQVLVERSLLGWAEIEFEVIR
                     DAADTAIAICGMENLDPMGVHTGDSIVVAPIQTLPDVVVQRLRRCALRIARALKLEGG
                     CNVQLAVSPDGADYRVIEVNPRVSRSSALASKATGYPIARVAALVALGRRLDEMRNPA
                     TGGGSAAFEPAVDYVVVKLPRWPFDKFPLAERGLGVQMKATGEAMAIDREFGPALLKA
                     VRSLEPRGRGWLWEDPAWRLAERAPADLGMFLAASDTRLWRMVGLLRHERTDAAGISA
                     VTGIAPWFTERLAELVASERALVGMPLAEAKRCGFGDAEVATVSGVPAAAVRRARVRA
                     GLAPAYRRVDTCAGEFPAETPYFYSSYADPEVPPPADRRSVIVVGSGPIRIGQGIEFD
                     YCSVRAAW"
     gene            complement(1726..2886)
                     /gene="carA"
                     /locus_tag="VGO32_03330"
     CDS             complement(1726..2886)
                     /gene="carA"
                     /locus_tag="VGO32_03330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV7199816.1"
                     /translation="MTTIASLPTTTPPDVPGPASFSGPGRLALEGGTVHAGVLRGGAS
                     SAGGDLIFTTAATGWGEILTDPSFAGQIVVLTHPMVGNYRIDRSELESDRVHARGLVV
                     TRLVTPPDGPGVSVEELLIEAGIPAIEGIDTRALTLELRRGADRRTVIRPADVASERA
                     VADAAASPAWEAVDHVAAVAPQTPYQVPPDGSRRGRAVLVDYGVKRSLLRALTSRGLE
                     VIGLPPDASAADVAAHGPDLVVLSPGPGDPSRMAPQIATVADLLARAVAGGPSLLGIC
                     LGHQLLGLAAGAPTRRLAVGHHGGNHGVIETASGQVTIGAHNHEVEVIDHPALAAAGL
                     QVSHRDLNDGTVEGLVHVGGRVASVQFHPEGAPGPTDAAGLFDQALAVANLA"
     gene            3015..3656
                     /locus_tag="VGO32_03335"
     CDS             3015..3656
                     /locus_tag="VGO32_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV7199817.1"
                     /translation="MTSPEVNLPPMRASAVTLVDTFVPRVDGRTAQIARDLVLIVAGA
                     ALTALAAQVSFTIGGNPVPYTLQTAAVLVTGTALGAGRGFASMLMYVAAGALGLGVFA
                     DGGSGLTAETGGLAPTIGYLFAFIVAATLCGRLAQRQWERTRTGAFLLMLLGSFVIYL
                     IGVPILAATTGMAFSEAIYWGAIVFIPWDLAKVVVATFAFPFAWRLAGDRTGE"
     gene            3653..4450
                     /locus_tag="VGO32_03340"
     CDS             3653..4450
                     /locus_tag="VGO32_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV7199818.1"
                     /translation="MTVNLDDEDSRASLTAEEFDRTWIEAHVAEERRSAAFLGDIREA
                     IFGIQDGLVSTVAVASTVAGATADKYAILVAAIAAGLAGMFSMAAGEYLSSKSQREIF
                     EAQIAGERDEVVNRPGEAQAEVAYMFEEDGLPPAEAAEVAGIVSRHPAVLLRTMVEKE
                     LSLHVEEHAGSPMQGALIMGGAFGIGALVPILPYLLLPTPEAIWASLIATGVVLFTVG
                     VAKARWTRRSPLRSGVEILAIGALAGVVGYFFGNILPPLLGAPAVGV"
     gene            complement(4453..4857)
                     /locus_tag="VGO32_03345"
     CDS             complement(4453..4857)
                     /locus_tag="VGO32_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV7199819.1"
                     /translation="MRARAGVVALALVLATSIGAGCQPAPTSVRIEIVIHYSHFTPSA
                     ITVPHGVPVRFVLISQDPIQHEWLIGDAAFHERHRHGTEPQHGARPDEVTIPPFATVE
                     TTLTFDQPGELAFICHVPRHEAYGMVGTLRIT"
     gene            complement(4862..5917)
                     /locus_tag="VGO32_03350"
     CDS             complement(4862..5917)
                     /locus_tag="VGO32_03350"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEV7199820.1"
                     /translation="MRRLVPLAAAMAVLLLTAGPTFACGGLIGPNGAVNLLRTTTFAG
                     YHDGVEHYVTSFAFAGGEGEFGSLVPLPDVPTSVERGGDWTLQRLILETEPQPLFLAE
                     DAALAGAARAEVLLEVRIDALDITILRGGGPEVGTWATEHGFRLPPDAPEVLDFYASR
                     SPIFLAAVFDADAAATRGQAVGDGTPVHLTIPTDDPWVPLRILALGKQDAETVEADVY
                     LLTDQRPALLPAPETAFAIDGMELAHSGRASDQLLTDLRSDKGMDWVPDQAWLTKVRI
                     NSAAPDVTFDLAIDAGGGLPSLVDAGLKPSGPAEMLWATSRAWGPLVLVIALPLLAAW
                     FAATIVTRRAAPPPAGA"
     gene            complement(6017..6283)
                     /locus_tag="VGO32_03355"
     CDS             complement(6017..6283)
                     /locus_tag="VGO32_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HEV7199821.1"
                     /translation="MSEGHDPTQAIGTVTHYFGHLSVAAVTLTDTLRVGERVHISGHT
                     TDLEQTVDSMEVEHAKVESAGPGDDVALHVNDHVREGDRIHREA"
     gene            complement(6287..6694)
                     /locus_tag="VGO32_03360"
     CDS             complement(6287..6694)
                     /locus_tag="VGO32_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV7199822.1"
                     /translation="MAPPLSEPLLEFALSLPEAWEDHPWGEDVAKVGKRVFVFFGMPD
                     AERPFGMTVKLPDSYDLAMSLPWAKNPGYRMDRGHWVWLQPPKDAPLEMLTEWIEESY
                     RAVAPKSLVSQLPIAQPRLDGETGADQSGPKED"
     gene            complement(6694..7542)
                     /locus_tag="VGO32_03365"
     CDS             complement(6694..7542)
                     /locus_tag="VGO32_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7199823.1"
                     /translation="MAHNLVRNFDQPDDRREFSRGSLDLIKVGPLTVGRETLHPGWRW
                     SVDVKPIAGTERCEFHHVGIQVSGRWACEDREGNVVEVGPGEIFDTPPGHDAWVVGDE
                     PCVTLDFQGISDWAMRSSASRVLTTVLFADIVESTPLVEQIGDPAWRRLLDQYRETVG
                     IILAGHHGVLVDTAGDGILARFDSPLAAVRSAVGITEAAERLEIRTRVGVHTGEVELT
                     DDALTGLAVHIGARVLAEAGPGEVVVTATTHDLTSDAGLAYEPRGSVVLRGITGARSL
                     YAIRPG"
     gene            complement(7562..>8806)
                     /locus_tag="VGO32_03370"
     CDS             complement(7562..>8806)
                     /locus_tag="VGO32_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEV7199824.1"
                     /translation="VILYQEQVMQIAITVCGYSATEADIFRKAMGSHRSHQAMQRERD
                     RFIAGALRTGLTKTDAEDLFRRCSAFAEFGFARAHAAAFAKITYDTAWLRLQYPAHYL
                     AGLLNNQPLGFYSPATLVNDARRHGLSVLPVDVNRSAWEHETERLAETGDHRRDFAVR
                     LGMRQVKGIDDAARTAIARERAIGPYRGMRDFVARTGLLEPVVERLVAIGAFDWTDTP
                     RRELLWQLRATLPDADPDRPPLGLTDAAAGEVGATLPPMSRAERVAADYRELGLSPTT
                     HAVELFRERLTERGVRTVRGAGEQRNGSMIRMGGVSTSIQHPMTAKGFVFLALEDETG
                     MMNVTLRPDVYRRYRAVLHRHPLLVITGRLQVEGAVLNVVATHLEPVEAVIAEPADTD
                     AASPERDREARFDLTRQRRMFR"
BASE COUNT         1319 a         3047 c         3196 g         1244 t
ORIGIN      
        1 ccacgcggcg cgcaccgagc agtagtcgaa ctcgatcccc tgcccgatcc ggatcgggcc
       61 cgagccgacg acgatcaccg agcggcggtc ggccggcggc ggcacctccg ggtccgcgta
      121 cgacgagtag aagtacggcg tctcggccgg gaactcgccg gcacaggtgt ccacccgtcg
      181 gtacgcggga gccaggccgg cccgaacccg ggcccgacga acggcggcgg ccgggacgcc
      241 cgacacggtc gcgacctccg cgtccccgaa tccgcaacgc ttggcctcgg ccagcggcat
      301 gccgaccagc gcgcgttcgg acgcgaccag ctccgcgagg cgctcggtga accacggggc
      361 gatacccgtg acggccgaga tccctgccgc gtcggtccgc tcgtggcgga ggagtcccac
      421 catccgccac aggcgcgtgt cggaggcggc gaggaacatg cccagatccg ccggcgcgcg
      481 ctcggcgagg cgccatgcgg ggtcctccca cagccagccg cgaccccgcg gctcgagcga
      541 gcggaccgcc ttcaggaggg ccggcccgaa ctcacggtcg atggccatcg cctcgccggt
      601 ggccttcatc tggacaccga ggccgcgctc ggcgagcggg aacttgtcga acggccagcg
      661 cggcagtttg acgacgacgt agtcgaccgc cggctcaaag gcggcggatc ccccgccggt
      721 ggccgggttg cgcatctcgt ccagtcgcct gcccagcgcg acgagggcgg ccacccgggc
      781 gatgggatag ccggtcgcct tggacgcgag ggccgaggaa cgcgagacgc gggggttgac
      841 ctcgatgacc cggtagtcgg cgccatccgg actcaccgcc agctgaacgt tgcagccgcc
      901 ctccagcttc aacgcccggg cgatccgcag cgcgcagcgc ctaagccgct ggacgacgac
      961 gtcgggcagg gtctggatcg gggcgaccac gatcgagtcg ccggtgtgga cgcccatcgg
     1021 atcgaggttc tccatcccgc agatcgcgat cgcggtgtcg gcggcatcgc gaatgacctc
     1081 gaactcgatc tcggcccagc ccagcaggct gcgctcgacg aggacctggc cgatggggct
     1141 ggctgccagc cctcgcgcaa tcgccgtccg ggcctcgtca cgacccgacg cgaagccgcc
     1201 cccgccgccg cccagggtga aggcggggcg gaccacgatc ggcgcgccaa ggtcgtcgac
     1261 gaacacgagg cccgcctcga ccgactccac gaccgcggac tcggggaccg gctctccgat
     1321 ggactggacg agcgaccgga acccgccgcg gtcttccgcc gcgcggaccg cggtcagggg
     1381 cgtgcccagc acggcgactc cgtatcgctc gagcacaccg gcgtcgtcca ggccgacggc
     1441 gaggttgagt gccgtctggc caccgagggt gggtagcagg gcgtcgggcc ggtgctcggc
     1501 gatcacacgc tcgaggtgcg gcaccgtgag cggaccgatg acgaccgtgc cgccgacgtc
     1561 cgggtcggtc atgaccgtgg ccgggttcga gttcacgagc acggtgtcga ttccctcgcc
     1621 ccggagggcg aggcaggcct gtaccccggc atagtcgaac tcggcagcct gaccgatgac
     1681 gataggcccc gagccgatga tcaggacacg gcgccggtcc atcggtcagg ccaggttggc
     1741 aacggccagg gcctggtcga acaggcccgc ggcatcggtc ggccccgggg caccttcggg
     1801 atggaactgg accgatgcca cccggccccc gacgtggacg agtccttcca ccgtcccgtc
     1861 gttcaggtcc cggtgactga cctgcagtcc tgcggccgcc agggccgggt ggtcgatgac
     1921 ctcgacctca tggttgtggg cgccgatcgt gacctgcccg ctggcggtct cgatgacccc
     1981 gtggtttccg ccgtggtggc caacggccaa ccgccgggtc ggcgccccag cagccagccc
     2041 gagaagctgg tggccgaggc agatcccgag cagcgatggg ccaccggcaa ccgcccgtgc
     2101 cagcaggtcg gccacggtcg cgatctgggg cgccatgcgg gacgggtcgc ccggtcccgg
     2161 ggagaggacc acgagatccg gaccgtgggc cgccacgtcg gccgcagaag catccggtgg
     2221 cagcccgatg acctccagcc cacggctggt cagggcgcga agcagggacc gcttgacacc
     2281 atagtcgacg aggacggccc gcccacggcg cgacccgtcc ggcgggacct gatagggcgt
     2341 ctgcggagcg accgccgcga cgtggtcgac ggcctcccag gcgggcgagg cggcggcatc
     2401 ggcaaccgcg cgctcggagg cgacgtcggc cggtcggatc acggtccgac ggtcggcgcc
     2461 gcgacggagt tcgagggtca gggcacgggt gtcgatgccc tcgatcgccg ggatgccggc
     2521 ctcgatcagc agctcctcca ccgagacgcc cgggccgtca ggcggggtca cgaggcgggt
     2581 caccaccagg ccgcgggcgt ggacgcggtc ggattcgagc tcggatcggt cgatgcggta
     2641 gttgccgacc attggatggg tgaggaccac gatctggccg gcgaaggagg ggtcggtcag
     2701 gatctcgccc cagccggtgg cagccgtggt gaagatcaga tcaccgccgg ccgaagacgc
     2761 cccgccacgg aggacgccgg cgtggacggt cccgccctcg agcgccagcc ggccgggacc
     2821 cgaaaacgac gccggtcccg gtacgtcggg cggcgtcgtg gtgggcaagc tggctatggt
     2881 cgtcatcgcg ctccttcccg gcctcacggg gccggactta aaggaccgga cgagtgtacc
     2941 ggagccaacc gctgggcggc agcccggagt tgccggcggg actacactcg ccgccacaga
     3001 aaagggagga gcggatgacc agcccagagg tgaacctgcc gcccatgcgc gcgagcgccg
     3061 tcacgctggt cgacaccttc gtcccgcgtg tcgacggccg caccgcccag atcgcgcgcg
     3121 acctggtgct gatcgtggcc ggcgccgccc tgaccgccct tgccgcgcag gtctcgttca
     3181 cgatcggcgg caacccggtt ccatacacgc tccagaccgc ggccgtgctg gtcaccggca
     3241 ccgccctcgg ggcgggacgc gggttcgcgt ccatgctcat gtacgtcgcc gccggcgcgc
     3301 tcggcctcgg cgtgttcgcg gacggcggaa gcggcctcac ggccgagacc ggcggcctgg
     3361 cgccgaccat cggctacctc ttcgcgttca tcgtggccgc aacgctgtgt gggcggctgg
     3421 cccagcggca gtgggagcgg acccggaccg gagccttcct gctcatgctc ctcgggtcgt
     3481 tcgtgatcta tctcatcggt gtcccgatcc tggccgccac gacggggatg gccttctcgg
     3541 aggccatcta ctggggtgcc atcgtgttca tcccgtggga cctggccaag gtcgtggtcg
     3601 ccaccttcgc cttcccgttc gcctggcgac tggccggaga ccggaccggg gagtgaccgt
     3661 caacctcgac gacgaggaca gccgcgcgtc gctgaccgcc gaggagttcg accgaacctg
     3721 gatcgaggcc cacgtcgcgg aggagcggcg gagcgcggca ttcctgggcg acatccggga
     3781 ggcgatcttc gggatccagg acggcctggt cagcacggtc gcggtggcca gcacggtggc
     3841 gggggcgacg gccgacaagt acgcgatcct ggtggcggcc atcgctgccg ggctggccgg
     3901 gatgttcagc atggcggccg gcgagtacct gtcgagcaag agccagcgcg agatcttcga
     3961 ggcccagatc gccggtgagc gggacgaggt cgtaaaccgg ccgggcgagg cccaggccga
     4021 ggtcgcgtac atgttcgagg aggacggcct tccgccagcc gaagcggcgg aggtggccgg
     4081 gatcgtgagc cgccatcccg ccgtgctgct ccggacgatg gtcgagaagg agctctcgct
     4141 ccatgttgag gagcacgccg gatcgccgat gcagggcgcc ctgatcatgg gcggagcgtt
     4201 cggcatcggg gccctggtcc cgatcctccc ctacctcttg ctgcccacgc cggaggcaat
     4261 ctgggcctcg ctcatcgcga caggggtcgt cctgttcacg gtcggagtgg ccaaggcccg
     4321 gtggacccgg cgcagcccgc tccgatcagg cgtcgagatc ctggccatcg gcgcgctggc
     4381 cggggtggtc ggctacttct tcggcaatat cctgccaccg ctcctcggag cgccagccgt
     4441 cggggtctga gctcaggtga ttctgagggt gccgaccatc ccataggcct cgtggcgggg
     4501 gacgtggcag atgaacgcca gctcgcccgg ctgatcgaag gtgagggttg tctccactgt
     4561 cgcgaacggc gggatggtga cttcgtccgg ccgcgcaccg tgctgcggct ccgtcccatg
     4621 ccgatgccgc tcgtggaatg ccgcgtcgcc gatcagccac tcgtgctgga tcgggtcctg
     4681 gctgatgagc acgaaccgaa ctggaacgcc atgcggaacc gtgatcgcgg acggggtgaa
     4741 gtgcgagtag tggatcacga tctcgatccg gaccgatgtc ggcgcgggct ggcagcccgc
     4801 gccgatggac gtcgccagca cgagggcgag cgccaccacc ccggcccgcg cacgcatccc
     4861 gtcaggcgcc cgccggcggg ggcgccgctc gtcgggtcac gatcgtggcc gcgaaccacg
     4921 ccgcgagcag gggcaaggca atcacgagga cgagtggtcc ccaggcacgc gaggtggccc
     4981 acagcatctc cgcggggccc gagggcttga ggccggcgtc caccagcgac ggcaggccgc
     5041 ctccggcgtc gatcgccagg tcaaaggtga cgtccggcgc cgcgctgttg atgcgcacct
     5101 tggtgagcca cgcctggtcg ggcacccagt ccatgccctt gtcggagcgg agatcggtca
     5161 ggagctgatc gcttgcgcgg ccgctgtggg ccagctccat gccgtcgatg gcgaacgccg
     5221 tttccggcgc aggaaggagc gccggccgct gatcggtcag gagatagacg tcggcctcga
     5281 ccgtctccgc gtcctgcttg ccaagggcca ggatccggag cgggacccac gggtcgtcgg
     5341 tcgggatggt gaggtgaacc ggcgtgccgt cgccgaccgc ctggccgcga gtcgcggccg
     5401 catcggcatc aaagaccgcg gccaggaaga tcggggagcg gctggcgtag aagtccagga
     5461 cctcgggagc gtcgggcggc agtcggaagc cgtgttcggt ggcccacgtg ccaacctccg
     5521 gtccgccacc gcggagaatc gtgatgtcca gcgcgtcgat ccggacttcg agcaggacct
     5581 cggcgcgggc tgcgccggcg agggccgcgt cctcggccag gaagagcggc tgcggctccg
     5641 tctcgaggat gaggcgctgg agcgtccagt ccccgccacg ctcgacggag gttggcacgt
     5701 ccggcagcgg caccagcgag ccgaactcgc cctcgccgcc ggcgaacgcg aacgaggtta
     5761 cgtagtgctc gacgccgtcg tggtagccgg cgaacgtcgt cgtccgcagc aggttgacgg
     5821 ccccattcgg gccgatcagg ccgccgcagg caaaggttgg cccggcggtg aggagcagga
     5881 cggccatggc agccgccagg ggcacgagac gtcgcatcgg acgctacctc catcgggcgg
     5941 cccggctgct gaccgcgatt ggttgatggc ggcatgacgg cgctgagccc ctggaggttc
     6001 ccccggcgtg ggcgagctag gcctcgcggt ggatgcggtc tccctcgcgc acgtgatcgt
     6061 tcacgtggag cgccacgtcg tcaccgggac cggcggactc gaccttggcg tgctcgacct
     6121 ccatcgagtc gacggtctgc tcgagatcgg tcgtgtgccc gctgatgtgg acgcgctcgc
     6181 ccacgcggag cgtatcggtc agcgtcacgg ctgccacgga caggtggccg aagtagtggg
     6241 tgacggtccc gatcgcctgg gtcggatcgt gtccctcgga catcggtcag tcctccttgg
     6301 gtccactctg gtccgccccg gtctcaccgt caagacgagg ctgtgcgatc gggagctggc
     6361 taacgagaga cttgggcgcg actgcccgat agctctcctc gatccactca gtgagcatct
     6421 cgaggggagc gtccttcggc ggctggagcc atacccagtg gccgcggtcc atgcggtagc
     6481 ccgggttctt cgcccagggc aggctcattg cgaggtcgta cgagtcgggt agcttcaccg
     6541 tcatcccgaa gggccgctcg gcatccggca tgccgaagaa gacgaacacc cttttcccga
     6601 ccttggccac gtcctcgccc cacgggtggt cctcccaggc ctcgggcagg ctcaatgcga
     6661 actcgaggag cggctcggag agaggaggag ccatcacccc ggccggatgg cgtacaggct
     6721 acgggcgccg gtaatcccac gcaggacgac cgatccgcgc ggctcgtagg ccagcccggc
     6781 atccgaggtc aggtcatggg tggtggccgt gacgacgacc tcaccgggcc ctgcttcggc
     6841 gagaacccgc gcgccgatat gcaccgccag gccggtcagt gcgtcatcgg tcagctccac
     6901 ctcgccggta tggacgccca cgcgtgttcg gatctccagc cgctcggcag cctcggtgat
     6961 gcccaccgcg gaccggaccg cggccagcgg actatcgaag cgggccagga tcccgtcgcc
     7021 ggccgtgtcg accaggacgc cgtgatgccc ggccaggatg attccgacgg tctcccgata
     7081 ctggtcgagc agccgtcgcc aggcggggtc gccgatctgc tcgacgagcg gggtggactc
     7141 tacgatgtcc gcgaagagga cggtggtgag gactcgtgaa gcggatgagc gcatggccca
     7201 gtcggagatg ccctggaagt cgagggtgac gcagggctcg tcgcccacaa cccaggcatc
     7261 gtggccgggc ggggtatcga agatctcgcc cggcccaacc tcgaccacgt tcccctcgcg
     7321 gtcctcgcac gcccaccgtc cgctgacctg gatcccgacg tggtggaact cgcagcgctc
     7381 ggtaccggcg atgggcttca cgtccaccga ccagcgccag ccgggatgca gggtttcccg
     7441 tccgaccgtg agcggaccga ccttgatcag gtcgagcgat cctctcgaga actcgcgtcg
     7501 gtcgtcgggc tggtcgaaat ttcggacgag gttgtgcgcc atcgatcgag tgtagcgttg
     7561 gtcagcggaa catccggcgc tggcgagtga ggtcgaaccg cgcctcgcga tcgcgctccg
     7621 gcgaggcggc atcggtgtcg gccggctcgg cgatgaccgc ctccaccggc tcgaggtggg
     7681 tggccaccac gttcaggacc gcgccctcca cctggagccg gccggtgatg accagcagcg
     7741 ggtggcggtg gaggaccgcc cggtagcggc ggtagacgtc ggggcggagg gtgacgttca
     7801 tcatcccggt ctcgtcctcg agggcgagga acacgaaccc cttggccgtc atcgggtgct
     7861 ggatggaggt cgagacccca cccatccgga tcatcgaccc gtttcgctgc tcgccggcgc
     7921 cacggacggt gcgcacgcca cgctcggtga gccgctcacg gaacagctcc accgcgtggg
     7981 tcgtcggcga caggcccagc tcgcggtagt cggcggccac ccgttcggca cggctcatgg
     8041 gcggcagggt cgcccccacc tccccggcgg cggcatcggt caggcccagc gggggccgat
     8101 ccggatcggc atcgggcagg gtggcgcgga gctgccagag cagctcgcgg cgcggcgtat
     8161 cggtccagtc gaaggccccg atcgccacca gccgctcaac gaccggctcg agcaggccgg
     8221 tacgggccac gaagtcgcgc atgccccggt acggaccgat ggcccgctcc cgcgcgatcg
     8281 cggttcgggc ggcatcgtcg atgcccttca cctggcgcat gcccaggcgc acggcgaagt
     8341 cacggcggtg gtccccggtc tcggccagcc gctcggtctc gtgctcccag gccgagcggt
     8401 tgacgtccac cggcaggacg ctcaggccgt ggcggcgcgc gtcgttgacc agggtcgcgg
     8461 gtgagtagaa gcccagcggc tggttgttca gcagcccggc caggtagtgg gccgggtact
     8521 gcagccggag ccatgccgtg tcgtacgtga tcttggcgaa ggccgcggca tgggcccggg
     8581 cgaagccgaa ctcggcgaag gcgctgcaac ggcggaagag gtcctccgca tcggtcttgg
     8641 tcaaacccgt tcgtaaggcg ccggcaatga accgatcccg ctcccgctgc atggcctggt
     8701 gggaccgatg cgagcccatc gccttgcgga agatgtcggc ctcggtggcg gagtagccgc
     8761 agaccgtgat cgcgatctgc atgacctgct cctggtacag gatcac
//
LOCUS       DASYGU010000127         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1719708, whole genome shotgun sequence.
ACCESSION   DASYGU010000127 DASYGU010000000
VERSION     DASYGU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719708"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGO32_03375"
     CDS             <1..542
                     /locus_tag="VGO32_03375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV7199825.1"
                     /translation="PLIRLLSVAMPIPGAAPISVYALVGAPALIGAILVARAIGASRQ
                     DLGLGRPARPVEVVLSTLIGVPLGLLARGVVGLAPISVEGVHPILFVAVAVPFVVLLE
                     ELVFRGLLQRVATSRSRLIGIVAPSVLYAAMYFGSGSASVVLFMGITGIIFGVMVSRQ
                     HSLWGVLGAHMILRVLVQI"
     gene            complement(553..1467)
                     /locus_tag="VGO32_03380"
     CDS             complement(553..1467)
                     /locus_tag="VGO32_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV7199826.1"
                     /translation="MTVKVIFIGGAGRSGSTLVDRVIGQIPGYASAGELRTLWDAGLI
                     ENRLCGCGQPFGDCPFWRQVGDLAFGGWSNTNPRDMEAALASLSYVRALRQLVVGSPP
                     SLDPEHSGVLGRLYEGLSAAAAGATIIDSSKGPRYALMLTAVPGIDLRLIHLVRDSRG
                     VAYSWSKEVARPDTPGRAVQMPRFGASAASARWMVQNSYTELLGRRVPVTRLRYETFI
                     ENPTGELLRVFRDLGEDVPPAALNFIGPDSARVGPSHTVMGNPMRMTTGEIRLVADSA
                     WRTALPRLPRAEVTALTFPLLVRYGYRV"
     gene            complement(1464..>2386)
                     /locus_tag="VGO32_03385"
     CDS             complement(1464..>2386)
                     /locus_tag="VGO32_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEV7199827.1"
                     /translation="YELAAPITISGDGLALVGVNVGNAAGYSEAALGSQIVPADGFPD
                     DAFLVNATAEAYGPLISLIHVDGMRRAQGMNIEGSRPTVSLNAVSQSSGVGIRFAGET
                     TGQRPYDGFVLFNRVFEGLGIGILHDQRSGDMLIEGNVVFRNGGDGFLSRGASQMYSL
                     NHAYNNAGVGIRLVPGCVRTRLTSNKWEGNDLGGLSIEGGSGFTVIGDTFADNDSPSG
                     TSAAHIELGVRGPTETIGVMLSNLAFGKGDDGNPYLIHFGSLARDVHVGPVTSNGGYR
                     TSPYLQDPGSEVQFYGPIPDPIVTDTGASN"
BASE COUNT          379 a          784 c          809 g          414 t
ORIGIN      
        1 tgccgctgat caggctgctg agcgtggcga tgccgattcc cggtgccgcc ccgatctccg
       61 tctatgcgct ggtgggggcg cctgccctca tcggggccat cctcgttgct cgagcgattg
      121 gcgcatcgcg gcaggacctc gggcttggac gtccggcgag gccggtggaa gtcgtcctgt
      181 caaccctgat cggcgtcccg ctgggtctgt tggcccgcgg cgtggtcggg ttggcgccga
      241 tatcggtcga gggtgttcac ccaatcctgt tcgtcgccgt cgcggtgcca ttcgtcgtgc
      301 tcctcgagga gctggtgttc cgcggcctcc tgcagcgggt ggcgacctcc cggtcgcggc
      361 tcatcggcat cgtcgcaccc agcgtcctgt atgccgcgat gtacttcggc tcaggttccg
      421 catcggtcgt cctgttcatg ggaatcaccg ggatcatctt tggcgtgatg gtcagccgcc
      481 agcattcgct gtggggtgtg cttggcgcgc acatgatcct tcgggtgctg gtccagatct
      541 gaaggacggt agctacaccc ggtacccata gcggacaagg agcggaaagg tcagggccgt
      601 gacctcggcc cgcggaagtc tcggaagggc ggtgcgccag gcgctgtctg ccacgagccg
      661 aatttcgccg gtcgtcatgc gcatggggtt gcccatgacg gtgtggctcg ggcccacgcg
      721 ggcgctgtcc ggtccgatga agttcagcgc ggcgggcggg acgtcctcgc ccaggtcccg
      781 gaacacgcgg agcagctcgc cggtcgggtt ttcgatgaag gtctcgtagc gcagccgcgt
      841 gaccggtacg cgccgaccga gaagttcggt gtacgagttt tggaccatcc accgcgccga
      901 cgccgcggat gcgccgaatc gcggcatctg aacggccctg ccgggcgtgt ccggccgggc
      961 gacctccttg gaccaggagt aggccacacc acggctgtca cgcacgagat ggatcaatcg
     1021 gagatcgatg ccgggcacgg cggtcaacat gagcgcgtac cgcggtccct tggacgagtc
     1081 gatgatcgtg gcgcccgccg cggcggccga gagtccctca tacaggcgcc ccagcacgcc
     1141 gctgtgctcg gggtcgagcg acggcggcga tccgacgacc agctggcgca gcgcccgaac
     1201 gtacgagagc gaggccaggg cggcctccat gtcgcgcggg ttggtgttgg accagccacc
     1261 gaaggcaagg tcgccgacct gccgccagaa cgggcagtcg ccaaatggct gtccacagcc
     1321 gcagagacgg ttctcgatca ggcccgcgtc ccacagcgtg cgaagctcgc cggccgacgc
     1381 ataacccgga atctgcccga tgacacggtc gaccagggtg ctgccgctgc ggccggcacc
     1441 cccgatgaag atgaccttga cggtcaattc gaggccccgg tatcggtcac gatcgggtct
     1501 gggatgggac cgtagaactg gacctcgctg ccgggatcct ggagatacgg gctggtgcgg
     1561 tatccgccgt tcgaggtcac gggacccacg tggacatcgc gggccagcga gccgaagtgg
     1621 atgagatacg gattcccatc gtcgcccttg ccgaaggcca ggttggacag catgacgccg
     1681 atcgtctcgg tcgggccccg gacgccgagc tcgatatgcg cagcgctcgt cccgctgggg
     1741 ctgtcgttgt cggcgaaggt gtcgccgatc accgtgaatc cgctcccgcc ctcgatgctg
     1801 aggccaccca ggtcgttgcc ctcccacttg ttgctggtca ggcgtgttcg cacacagccc
     1861 gggaccagcc gaatcccgac gccggcattg ttgtacgcgt ggttgagcga gtacatctgg
     1921 gaggctccac ggctgaggaa gccgtcgccg ccgttccgga agacgacatt tccctcgatc
     1981 agcatgtccc ccgaacgctg gtcatggagg atcccgatgc ccaacccctc aaacacccga
     2041 ttgaacagga cgaatccgtc gtagggacgc tgcccggtgg tctcgccggc aaaacgaatc
     2101 ccgaccccgc tcgactggga caccgcgttg agtgacacgg tcgggcggga cccctcgatg
     2161 ttcattccct gcgcgcgtct catcccatcg acgtgaatca ggctgatcag ggggccatat
     2221 gcctccgccg tcgcattgac caggaatgcg tcgtccggaa atccgtctgc gggcacgatc
     2281 tgcgagccga gggccgcctc ggaatagccg gcagcattgc caacgttcac gccgacgagg
     2341 gccaggccgt cacccgagat cgtaatcggc gccgcgagct cgtaga
//
LOCUS       DASYGU010000128         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_845047, whole genome shotgun sequence.
ACCESSION   DASYGU010000128 DASYGU010000000
VERSION     DASYGU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845047"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VGO32_03390"
     CDS             <1..754
                     /locus_tag="VGO32_03390"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEV7199828.1"
                     /translation="RYDLPLFLDACRFAENAWFIRTREAGYEATPIPDIVREMASLAD
                     GMTMSAKKDGLANMGGWLAMNDDGWAEQARNLLILTEGFPTYGGLAGRDLESIAAGLR
                     EVVDEDYLRYRIRSTEYLGDGVTAAGIPCIQPIGGHAVYLDAAALLPHIPPLAYPGQA
                     LAVALYREGGIRGCEIGTVMFGRHPDGSETPAPMELVRLAIPRRVYTQSHVDYVIEVA
                     RWVADRAADLRGLRITEQPSVLRHFTARFEPL"
     gene            843..1430
                     /locus_tag="VGO32_03395"
     CDS             843..1430
                     /locus_tag="VGO32_03395"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7199829.1"
                     /translation="MAAAHAATTVDLGTGDGRFVLAHAAAHPERLVLGIDASHAAMRE
                     ASRRAARVPARNGRPNALFVASALESLPPDLAGVADLVMVSFPWGTLRMAAAGHDRDL
                     TARLAGLIGPGGRLELLLADAERDGTPPVDLEALRDTYECLGFRLVLDRPATLDDAVA
                     AHSSWGKRLLRHPAPGRSARRIGLERSAAHPVTRG"
     gene            1440..2462
                     /locus_tag="VGO32_03400"
     CDS             1440..2462
                     /locus_tag="VGO32_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEV7199830.1"
                     /translation="MTIGQRVSNWRYALATANPPPGVPLDGVTKWLVVTRAAVFSMTV
                     TSGLIGGLLAVGADPAAVNYGYLALAIVGLVAAHAANNMINDYFDMEVGIDTDEYVRA
                     LYAPHPVLSGWLTKAQLRNAILVANAVDFAILLALTWVRGPLVVAFAAAGLFISVFYV
                     APPISLKKRGLGEPGVFLTWGPLMIVGTYFVATGTIPGWVWIASLPYAILVTTVLFGK
                     HIDKIIPDAERGIRTLPVILGERRARMVASGLMIAFYPIVIGPALVGWIGPWVVLVVL
                     AIPRLVEVLRQFAVPKPVTPPNAYVGWPLWFVGGAFIHTRRAGAMLILGLLLNILLPI
                     TLPWLT"
     gene            2450..>2813
                     /locus_tag="VGO32_03405"
     CDS             2450..>2813
                     /locus_tag="VGO32_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurase"
                     /protein_id="HEV7199831.1"
                     /translation="MADLSPTGVVEGIHLHGDRGEPMRVVADAELVAGHGLVGDRMAG
                     LGIPGTHVTFIGAEGVEAMVAETGIPLAPYQTRRNILVRGVDVPGLVGHRFQVGDAVC
                     YGVKECHPCIHLESVTQAG"
BASE COUNT          370 a          997 c          967 g          479 t
ORIGIN      
        1 ccgctacgac ctgccgctgt tcctggacgc gtgccgcttc gccgagaacg catggttcat
       61 ccggacccgc gaggcgggct acgaggccac cccgatcccg gacatcgtcc gcgagatggc
      121 ttccctggcg gacggcatga cgatgtcggc caagaaggac gggctggcga acatgggcgg
      181 ctggctggcc atgaacgacg acggatgggc cgagcaggct cgcaacctgc tgatcctgac
      241 cgagggcttt ccgacctatg gcgggctagc cggtcgggac ctggagtcca tcgcggctgg
      301 tctccgcgag gtggtcgacg aggattacct ccgctatcgg atccgttcga ccgagtacct
      361 cggcgacggg gttacggcgg cgggcatccc gtgcatccag cccatcggcg ggcacgcggt
      421 ctacctcgat gccgccgcgc tcctgcccca catccctccg ctggcgtacc ccggccaggc
      481 cctcgccgtc gcgctctacc gggaaggggg catccgcggc tgcgagatcg ggacggtgat
      541 gttcggtcgg catccggacg gcagcgagac gccggcgccg atggagctcg ttcgcctcgc
      601 catcccacgt cgggtgtaca cccagagcca cgtcgactac gtgatcgagg tcgcgcgctg
      661 ggtcgccgat cgggcggcgg acctgcgcgg gctccggatc accgagcagc cgagcgtgct
      721 gcgccatttc accgctcgct tcgagcctct gtgaggccgg cgcgccgggc cggtatcatc
      781 ggtccatgcg tctcctgact ggacgccgcc tcgccgaggt ggacgtcagc taactgaccg
      841 atatcgccgc ggcccatgcc gcgacgacgg tcgacctggg caccggtgac ggccggttcg
      901 tgctggccca cgccgcggcc caccccgagc ggctggtgct cggtatcgat gccagccatg
      961 ccgctatgag ggaggcgtcg cgtcgagcgg ctcgggtacc cgcccgtaac ggccggccga
     1021 atgccctgtt cgtcgcgtct gcgctcgaga gtctgcctcc ggacctcgcc ggcgtggccg
     1081 atctggtgat ggtctcgttc ccgtggggga ccctgcgcat ggcggcggcc ggccacgatc
     1141 gtgacctgac cgcccggctg gccggtttga tcggaccggg tggccggctc gagctcctgc
     1201 tcgccgacgc cgaacgcgac ggcaccccgc ccgtcgacct cgaggcgctg cgggatacct
     1261 acgagtgcct cggattccgt ctggtgctcg accgaccggc caccctggac gatgccgtcg
     1321 ccgcccattc gtcgtggggc aagcgactgc tccgtcaccc ggctccgggt cgatcggcgc
     1381 ggcggatcgg gctcgagcgg tccgcggccc atcccgtgac gcggggatag aatcggttcg
     1441 tgaccatcgg tcagcgcgtc tccaactggc ggtatgccct cgccaccgcc aacccaccgc
     1501 ccggcgtccc gctcgacggg gtgacgaagt ggctggtcgt gacccgcgcg gcggtgttca
     1561 gcatgaccgt cacgtcgggc ctgatcggcg gcttgctcgc cgtcggcgcc gacccggcgg
     1621 ccgtgaacta tggctacctc gccctcgcca ttgtcgggct ggtcgcggcc cacgccgcga
     1681 acaacatgat caacgactac ttcgacatgg aggtggggat cgacaccgac gagtacgtcc
     1741 gcgccctgta cgccccgcac ccggtcctgt cgggctggct gaccaaggcc cagctgcgga
     1801 acgcgatcct cgtcgccaac gcggttgact tcgcgatcct tctggccctg acctgggtcc
     1861 gaggcccgct ggtcgtggcc ttcgccgcgg ccgggttgtt catcagcgtt ttctacgttg
     1921 ccccgccgat cagcctcaag aagcgcgggc ttggcgagcc gggggtgttc ctgacctggg
     1981 gcccgctcat gatcgtgggg acctacttcg tggccaccgg cacgattccg ggctgggtgt
     2041 ggatcgcgtc gcttccctat gcgatcctgg tcacgaccgt cctgttcgga aagcacatcg
     2101 acaagatcat tcccgacgcc gagcggggga tccgcaccct tcccgtcatc ctgggcgagc
     2161 gacgggccag gatggtcgcc tcgggactga tgatcgcgtt ctatccgatc gtcatcggtc
     2221 cggcgctcgt gggctggatc ggtccgtggg tggtcctggt cgtgctcgcc atcccacgcc
     2281 tggtcgaggt tctccgccag ttcgccgtac ccaagcccgt gaccccgccc aacgcgtacg
     2341 tcggctggcc gctgtggttc gtgggcgggg cgttcatcca cacccgtcgg gcgggcgcga
     2401 tgctcatcct cggactcctc ctgaacatcc ttctgcccat caccttgccg tggctgacct
     2461 gagccccacc ggcgtggtgg agggcatcca cctccacggc gaccggggcg agccgatgcg
     2521 cgtcgtggct gatgccgagc tggtggcggg gcacgggctg gtgggggacc ggatggccgg
     2581 gctcggcatt cccggcaccc acgtgacctt catcggcgcc gaaggggtgg aggccatggt
     2641 cgccgagacg ggcatcccgc tcgcgccgta tcagacccga cgcaacatcc tggtccgggg
     2701 cgtggacgtg cccggcctcg tcggccatcg attccaggtc ggggacgcgg tgtgctacgg
     2761 cgtcaaggag tgccatccct gcatccatct cgagtcggtg acccaggccg gcg
//
LOCUS       DASYGU010000129         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_639599, whole genome shotgun sequence.
ACCESSION   DASYGU010000129 DASYGU010000000
VERSION     DASYGU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639599"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VGO32_03410"
     CDS             <1..1120
                     /locus_tag="VGO32_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199832.1"
                     /translation="HAASMAYLVQASAGASGTATMAQQANAADGWTAVTIVFTPGSGG
                     PPTSPSVTVNQAASQADPTNTSPISFTVVFSEPVTGFTGADVTISGTAGGTKAVNVSG
                     SGPTYTVAVSGMTTAGTVVASIPAGAATDADANGNTASTSTDNSVTWNPVVPPSGWTH
                     VGTGPLAATFGSSSTSTSVGYPAGIAAGDLLLLGCQGRHNSMDWSAPGFGSLIRTTLT
                     SGLRFEVLSAWAAGGESGSLTVTSTIGMNGWSCELSAFRGGLGAGSPLAAAPVAVAGK
                     GSTMTAPSVTTATGGALVTRWFASMDDNNHGNPSAGSLAFGGTAYHTTVGVDHAASMA
                     YLVQASAGASGTATMAQQANAADGWTAVTIVFTPGSGG"
     gene            complement(1153..1227)
                     /locus_tag="VGO32_03415"
     tRNA            complement(1153..1227)
                     /locus_tag="VGO32_03415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1193..1195),aa:Thr,seq:cgt)
     gene            complement(1218..2099)
                     /gene="nth"
                     /locus_tag="VGO32_03420"
     CDS             complement(1218..2099)
                     /gene="nth"
                     /locus_tag="VGO32_03420"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV7199833.1"
                     /translation="MPTRRSATRNRRLAQLRRIRRARIGPVLERLAERYGMPEWSGAR
                     LDPVAELVLTVLAQNTADTNSHRAFIALRAAYPSWDAVLAAPTDELADVIRPGGLAPT
                     KSRRIQQVLAEVHDASGGTWDLGFLGEWPLDEARAWLVGLPGIGRKTASIVLLFAFGR
                     PALPIDTHVYRVALRLGLIPPKTNLTRAHDLLEEVVPPDSMFAAHVLLIGHGREVCRA
                     PRPICGLCPLTDLCPYFALVQKGKARMAPIPRSLERPARYHDELLHGWAPPTIGSERV
                     IGENRTEALESRGAGPK"
     gene            complement(2099..3280)
                     /locus_tag="VGO32_03425"
     CDS             complement(2099..3280)
                     /locus_tag="VGO32_03425"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV7199834.1"
                     /translation="MSPALRVVVAPDSFGGALDSVAAAAAVARGWNSTRPDDEVVRIP
                     MADGGEGTLAAIAAALGDGVERRSATTLDPLGREVSADWLALDGGASAFIEMAGASGL
                     AHLAPAERTPATARLASTRGTGLLIRAALDAGVRRIVVGLGGSGTTDGGAGLMVELGL
                     RLLDARGEDLLDGGGALAQLDHVEVEGVDKRLTETEILIASDVTSPLIGPRGAAATFS
                     PQKGADPETVELLDMALGRWGAEIQRATGRDVSDVPGAGAAGGTTAGLLGFTTATLRP
                     GVEVVAELVGLAAACEGADLVITGEGRADEQTLAGKAATGVARQAGGAPVVLLCGALG
                     PGAEIVEASGAFAVIQPIVDRPMDLEASMADTERLLANASARLARSIGIGRRLGSRGW
                     R"
     gene            complement(3277..>4962)
                     /locus_tag="VGO32_03430"
     CDS             complement(3277..>4962)
                     /locus_tag="VGO32_03430"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEV7199835.1"
                     /translation="PSVEVRTAVRIGRQASEGIIEAVAEIGADLVIFGWGGPPRNEAR
                     RAVFSTTIDEVAREAPCDIAVVKQRGLGVVESILGPVRGGPNAELAVSLATDLARGFR
                     AELVVLHVVPPGGGLEAIERERTALAALVKRAARGVSVRDLVVEAPTVRGAILAEAAN
                     HQLVVVGASAQPTGLGSGRRLFGRLAETVASRAKATVMVVRTRQPVTPHTFVDLRADE
                     ASLPAADEAIEAGQSLPSIVDRWFAENTFHASEFRNLRSLVELKERNGLTVSVGLPTL
                     NEEKTIGLVIRRIRDALMDRVPLVDELLVIDSASTDRTVEIARELGVPVRQHDEILPE
                     LGSYPGKGEALWKSLYELRGDIVVWVDTDIRNIQPRFVYGLLGPLLREPRIQYVKGFY
                     HRPLKSGGAWQPEEGGRVTELTARPLINLFFPQLSGVIQPLAGEYAGRRELLESVPFF
                     TGYAVEFGLLVDVMDRVGLSGIGQVDLERRIHRNQSLIDLSRMSFVILKAAITKLEER
                     QQIELLTEMGETMKQIRVEETRFSLDIREVGDEIRPPMSSIPAYRARRAELRS"
BASE COUNT          739 a         1829 c         1724 g          670 t
ORIGIN      
        1 ccacgccgcc agcatggctt acctcgtgca ggccagcgcc ggcgccagcg gcacggccac
       61 catggcccag caggccaacg ccgccgacgg ctggaccgcg gtgaccatcg tcttcacccc
      121 gggcagtggc ggcccgccca ccagcccgag cgtgaccgtc aaccaggccg ccagccaggc
      181 tgacccgacc aacaccagcc cgatcagctt cacggtcgtg ttcagcgagc cggtgaccgg
      241 cttcaccggc gccgacgtca cgatcagcgg cactgccggc ggcacgaagg ccgtgaacgt
      301 cagcggcagc ggcccgactt acaccgtggc ggtcagcggc atgaccaccg ccggcaccgt
      361 ggtcgcctcc atcccggccg gtgcggccac cgatgcggac gccaacggca acaccgcctc
      421 gaccagcacc gacaacagcg tcacctggaa cccggtggtg ccgccgtccg gctggaccca
      481 cgtcggcacc ggcccgctgg cggccacctt cggctcctcc agcacgtcga ccagcgtcgg
      541 ctacccggcc ggcatcgccg ccggcgacct gctgctgctc ggctgccagg gccgccacaa
      601 ctcgatggac tggtcggcac ccggcttcgg cagcttgatc cggacgacgt tgaccagcgg
      661 cctgcgcttc gaggtcctct cggcctgggc cgcgggcggc gagtcgggca gtctgacggt
      721 gaccagcacg atcggcatga acggctggtc gtgtgagctc agcgccttcc gtggtgggct
      781 cggcgccggc tcaccgctcg ccgctgcgcc ggtcgcggtc gcgggcaagg gcagcaccat
      841 gaccgcgccg agcgtgacga ctgccaccgg cggcgcgctg gtcacgcgct ggttcgcgtc
      901 catggacgac aacaaccatg gcaacccctc ggccggcagc ctggctttcg gcggcaccgc
      961 ctaccacacc accgtcggcg ttgaccacgc cgccagcatg gcttacctcg tgcaggccag
     1021 cgccggcgcc agcggcacgg ccaccatggc ccagcaggcc aacgccgccg acggctggac
     1081 cgcggtgacc atcgtcttca ccccgggcag tggcggctga cgtcggccgg acgggattcc
     1141 tgagccgaat tgtggagccg aaggtgggat tcgaacccac gaccgacggt ttacgaaacc
     1201 gttgctctac ccctgagcta cttcggcccg gcgccgcgcg attctagcgc ctcggtccgg
     1261 ttctccccaa tgacccgttc ggagccgatg gtgggtgggg cccagccatg gagcagctcg
     1321 tcgtggtacc gagcgggtcg ctcgagagac cggggaatgg gtgccattcg ggctttcccc
     1381 ttctggacga gggcgaagta gggacacagg tcggtgagcg ggcagaggcc gcagatcggc
     1441 cgtggcgcgc ggcagacctc gcgtccgtgg ccgatgagca ggacgtgcgc cgcaaacatc
     1501 gaatcgggcg ggacgacctc ttcgagcagg tcgtgggcac gggtcaggtt ggtcttgggc
     1561 ggaatcagcc cgagccggag ggcgacccgg tagacgtggg tgtcgatcgg cagcgccggc
     1621 cgcccgaacg cgaacagcag cacgatcgag gccgtcttgc ggccgatccc gggcagtccc
     1681 accagccagg cacgggcctc gtcgagcggc cactccccga ggaagccgag gtcccaggtc
     1741 cccccggacg cgtcgtgcac ctcggccagg acctgctgga tgcggcgcga cttcgtcggc
     1801 gccaggccac cgggtcggat gacgtcggcc agctcgtcgg tcggcgcggc caggaccgca
     1861 tcccaggacg ggtaggctgc ccggagcgca atgaacgccc gatgcgagtt cgtgtcggcc
     1921 gtgttctggg caaggacggt gagcacgagc tcagcaaccg gatcgagacg tgcgccagac
     1981 cattcgggca tgccgtagcg ctcggcgagc cgttccagca ccggaccgat ccgagcccgc
     2041 cggatgcggc gcagctgcgc gagccgccga ttcctggtgg ccgatctccg ggtgggcatc
     2101 agcgccagcc gcgggaaccc aggcgcctgc cgatgccgat gctccgcgcc agccgcgcgc
     2161 tggcattcgc cagcagtcgc tcggtgtccg ccatcgaggc ctccaggtcc atcggtcggt
     2221 cgacgatggg ctggatgacc gcgaacgcgc cggaggcctc gacgatctcc gcccccggcc
     2281 cgagtgcccc gcacaggagg acgacggggg cgccgcccgc ctgacgggcg acgccggtcg
     2341 ccgccttgcc ggccagcgtc tgctcgtcgg cccggccctc gccggtgatg accaggtcgg
     2401 caccctcgca ggctgcggcc aacccgacca gctcggccac gacctcgaca ccggggcgaa
     2461 gtgtggccgt ggtgaatccc agcagcccgg cggtggtgcc tcccgcggca ccggccccgg
     2521 gcacgtcgga aacgtctcgg cctgtggccc gctgaatctc cgcgccccac cgaccgagcg
     2581 ccatgtcgag cagctcgacc gtctccgggt ccgcgccctt ctggggggag aaggtcgccg
     2641 ccgctccccg cggcccaatg agcgggctgg tgacgtcgga ggcgatcagg atctcggtct
     2701 ccgtcagccg cttgtccacg ccctccacct ccacgtgatc cagctgggcg agcgcgcctc
     2761 cgccgtcgag caggtcctcg ccgcgcgcgt cgagcagccg cagcccgagc tcgaccatga
     2821 ggcccgcgcc gccatcggtc gttcccgacc caccaagccc gaccacgatt cggcggacac
     2881 ccgcgtcgag cgccgcgcgg atgagcaacc ctgtcccacg ggtcgaggca aggcgggcag
     2941 tggcgggcgt gcgttcggcc ggggccagat gggcgaggcc actggcgccg gccatctcga
     3001 tgaacgccga cgcaccgcca tcgagcgcga gccagtcggc ggaaacctct cggccaaggg
     3061 gatcgagcgt ggtggcggag cgccgttcga cgccgtcgcc gagggccgct gcgatcgcgg
     3121 ccagcgtgcc ttcgccgcca tcggccatcg gaatccggac cacttcgtcg tccgggcggg
     3181 tcgagttcca gccacgggcc accgccgcgg cggccgccac cgagtcgagc gccccgccga
     3241 acgagtcggg ggcgacgacc acccgcaggg cggggctcac gaacgcagct ccgcccggcg
     3301 ggcgcgatag gcgggaatgg agctcatcgg cggacggatc tcgtcaccca cctcccggat
     3361 gtccaggctg aaccgcgttt cctcgacccg gatctgcttc atggtctccc ccatctcggt
     3421 cagcagctcg atctgctggc gctcttcgag cttggtgatc gccgccttga ggatgacgaa
     3481 ggacattcgg gacagatcga tcagtgactg gttgcggtgg atccggcgct cgagatcgac
     3541 ctggccgatg ccggacagtc cgacccggtc catgacatcc accagcagcc cgaactcgac
     3601 ggcgtagccg gtgaagaatg gcactgattc gagcagctcg cggcgcccgg cgtactcgcc
     3661 ggcgaggggc tgaatgaccc ccgacagctg ggggaagaac aggttgatga gcggccgcgc
     3721 ggtgagctcg gtgacgcggc cgccctcctc gggctgccac gcccctccgg acttcagcgg
     3781 acggtggtag aagcccttga cgtactggat ccgcggttcg cgaaggagtg gccccagcag
     3841 cccgtacacg aagcggggct ggatgttccg gatgtcggtg tcgacccaca ccacgatgtc
     3901 gcctcgcagc tcgtacaggc tcttccacag cgcctccccc ttgcccgggt agctgcccag
     3961 ttcggggagg atctcgtcgt gctggcggac cggcacgccc agctcgcggg cgatctcgac
     4021 cgtccgatcg gtcgatgccg agtcgatcac cagcagctcg tccaccagtg gaacacggtc
     4081 catcaaggca tcccggatgc ggcgaatgac gaggccgatc gtcttctcct cgttgagtgt
     4141 cggcaggccg acgctgacgg tcagcccgtt gcgctccttg agctcgacca ggctccgaag
     4201 gttgcggaac tcgcttgcgt ggaacgtgtt ctcggcgaac cagcggtcca cgatggaggg
     4261 caggctctgg ccggcttcga tcgcctcgtc ggcggccggc aggctcgcct cgtcggcccg
     4321 caggtccacg aacgtgtgcg gggtgaccgg ctgacgggtg cggacgacca tgaccgtggc
     4381 cttggcccga gaggcgaccg tctcggccag acggccgaac agccgccgtc ccgagccgag
     4441 gccggttggc tgggccgagg ccccgacgac cacgagctga tggttcgcgg cctcggccag
     4501 gatcgccccc cggacggtgg gcgcctccac gaccaggtcg cgcaccgaca cgccccgtgc
     4561 ggcgcgcttg accagggcgg cgagcgcggt ccgctcccgc tcgatggcct ccaggccccc
     4621 gcccgggggc acgacgtgca ggaccacgag ctcagcgcgg aacccgcgcg ccaggtcggt
     4681 ggccagggag acggccagct cggcgttcgg gccgccgcgg acgggaccca ggatcgactc
     4741 cacgacgccg agcccgcgct gcttcacgac cgcgatgtcg cacggcgcct cgcgggccac
     4801 ttcgtcgatg gtcgtcgaga acaccgcgcg ccgggcctcg ttccgcgggg gcccgcccca
     4861 tccaaagatg accaggtcgg cgccgatctc ggccacggcc tcgatgatcc cctcggatgc
     4921 ctggcggccg atccgcaccg cggtccggac ctcgaccgac gg
//
LOCUS       DASYGU010000130         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1153869, whole genome shotgun sequence.
ACCESSION   DASYGU010000130 DASYGU010000000
VERSION     DASYGU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153869"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VGO32_03435"
     CDS             <1..630
                     /locus_tag="VGO32_03435"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV7199836.1"
                     /translation="PDSRQYRRFRIRSGDTPDDFRMMAEVLRRRFGRAARLRRETGAV
                     EPSSVGVDEDAVDGQEWALPDLVIVDGGKGQLSAATRVLTDLGLDQIPVAGLAKRFEE
                     LFVPGQPDPIILPGKAPALYLVQRIRDEAHRFAITYHRQVRGRRALGSLFDDVPGIGP
                     ARRAALLRRFGSIRALRDASVEEIAAAPLMSEALAERLKSALAREGRLA"
     gene            complement(627..2225)
                     /locus_tag="VGO32_03440"
     CDS             complement(627..2225)
                     /locus_tag="VGO32_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEV7199837.1"
                     /translation="MFARKSLPIRRLRRALLGGAILAVAVAFGPWLSPGAALEVPPVG
                     GFYIQTSGPNAGVGIGDWYSSTTAGAGSGYNYLEITIPCGWPSATPLHFDLFSPEMNQ
                     VAGALGLGDEVRGGVYDSTQFELYGPGVSVGPGYALPAPGAGIPGSQVTYPTVATPET
                     WVRYRTMSPVVCGRYLLRSAVLAPQGDDDNGWRLRVGLDDDGNATTAPPANTDNPDGV
                     PGTNDEVVIGQVQITYQQDSGGVSCLTLYEYIPPGLASVTFHNFDMDGNTRVVYYAPS
                     DAYDPNGLTGGTAGTLSINAQWNQGTIARGGDVVANPEAGWWRVVSCLSSTNQFIQEA
                     QAGAGAYFVQPPIPTLVIAKTDGQTETMPGATLTYAISVLNTATGPTAGAAHSVVVTD
                     ALPAEATFVGCTIVAPATGSCSASAGVVTATLNGWINAGATAEVQVTVTVNGTASGTL
                     SDVAGATYRDGLGNPFPGVTASDVNTVNPLPPTPPPDLPNTSAAQPGTLDPVRAAWLG
                     LGLAALLVAVLLPLEGTFRRPRVF"
     gene            2428..3750
                     /gene="secD"
                     /locus_tag="VGO32_03445"
     CDS             2428..3750
                     /gene="secD"
                     /locus_tag="VGO32_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEV7199838.1"
                     /translation="MTWRRIAPWVILAITLLALYIDIPRKSTGATFLPLPDSIRTVLG
                     LDLQGGISVTLEVQSVSGEEPDPERVELARAIIERRVAGLGVTEPQVRTEVGANGEAR
                     IVVEVPGVSDRNQILQLVGSTGQLLFLDPMAETLTEGQNVVDLIGTGAISVLFDGGEI
                     QQGSVVPDTSQSGQVGVSFTLTPEAQEIWCNYTTGHVNQPGPIALDGEVITTPTIQQP
                     ICGGRTIITVGAGTAAVAEQTTLYNQLRFGALPVSLTVQEVTAIQPTLGRDFLVQAII
                     AGTVGLLLVLFFMIANYRLPGVLASLALIFYTLVVYAIFRIIPVTLTLAGVAAFILSI
                     GMAVDANILIFERMKEEIRAGKTLGPAIEAGFNRAWSSILDSNVSSLLIAIWLFWQGT
                     SVIRGFALVLGIGVLVSMFSAVVVTRTLLRMVTHREWGRRIEYYHVER"
     gene            3754..>4426
                     /locus_tag="VGO32_03450"
     CDS             3754..>4426
                     /locus_tag="VGO32_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199839.1"
                     /translation="MYDIVGKRRWYYFFSALITIPGVFFILIGGLKPSIDFTGGTVWQ
                     VRFLDDPSPDEVQAALVDLGHDEATVEAVDNGFLEIRLAPIEFPIVGGIPTPTPSPAG
                     SPTVSPSLSATATPSPAGSPTGSPGASPTATPSPTPTPTPSPTPSPSPTAEPTPEPPP
                     TIQGTPIEELVTELNAQFGFSETRSLDTVGPIIGAELVRSSLIIIIIGEAFIFLYLWY
                     RFRSIR"
BASE COUNT          654 a         1542 c         1539 g          691 t
ORIGIN      
        1 ccggacagcc ggcagtaccg ccggttccgg atccggagcg gcgacacccc ggacgacttt
       61 cggatgatgg ccgaggtcct gcgccgtcgg ttcggccgcg cggcccggct gcggcgcgag
      121 accggcgcgg tggagccgtc atcggtcggc gtggacgagg acgcggtgga cggccaggag
      181 tgggcgctgc ccgacctcgt catcgtggac ggcgggaagg gccagctgtc ggcggcgacc
      241 cgggtgctca ctgatctcgg gctcgaccag atcccggtcg ccgggctggc caagcggttc
      301 gaggagctgt tcgtccccgg ccagccggac ccgatcatcc tgcccgggaa ggcgccggcc
      361 ctgtacctcg tccaacgcat tcgggacgag gcccatcggt tcgcgatcac gtaccaccgc
      421 caggtccgtg gtcggagggc gctgggcagc ctgttcgacg acgtaccggg catcggtccg
      481 gcccgccgcg ccgccctcct gcgccggttc ggcagcatcc gggcgctgcg cgacgcatcg
      541 gtcgaggaga tcgccgcggc gccgctgatg agcgaggctc tggccgaacg gctgaagagc
      601 gcgctggcgc gcgaggggcg gctggcctag aagacccgcg ggcggcggaa cgtgccctcg
      661 agcgggagga gcaccgcgac cagcagcgcg gcgaggccga gtcccagcca cgcggcgcgg
      721 accgggtcca gggttcccgg ctgggcggcg ctggtgttgg gcaggtccgg cggcggggtg
      781 ggcggcaggg ggttcaccgt gttcacgtca ctcgccgtca cgcccgggaa cgggttcccg
      841 agcccgtcgc gataggtcgc tcccgccacg tcggacaggg tcccgctggc cgtgccgttg
      901 acggtgaccg tgacctggac ctcggccgtg gcaccggcgt tgatccagcc gttgagggtg
      961 gcggtcacca cccctgcgct cgccgagcat gacccggtgg ccggggcgac gatggtgcag
     1021 cccacgaacg tggcctcggc cgggagcgcg tcggtcacga ccaccgaatg ggcagcgccg
     1081 gcggtcggac ccgtcgccgt gttcaggacc gagatcgcat acgtgagcgt ggcgccgggc
     1141 atggtttcgg tctggccatc ggtcttcgcg atgaccagcg tcgggatcgg cggctgcacg
     1201 aagtaggcgc cggcaccggc ctgcgcctcc tggatgaact ggttggtgga gctcagacag
     1261 ctcaccaccc gccaccagcc ggcctccggg ttggccacca cgtcgccccc gcgcgcgatg
     1321 gttccctggt tccactgcgc gttgatggag agcgtgcccg ccgttccgcc ggtcagccca
     1381 ttcgggtcgt acgcgtccga cggggcgtag tagacgaccc gcgtgttgcc gtccatgtcg
     1441 aagttgtgga aggtgacgct cgccagacca ggcgggatgt actcgtacag cgtgaggcac
     1501 gacacgccgc cgctgtcctg ctggtacgtg atctggacct ggccgatgac cacctcgtca
     1561 ttcgtgcccg gcacgccgtc ggggttgtcg gtgttggccg gcggcgcggt ggtggcgttc
     1621 ccgtcgtcat cgaggcccac ccgcaatcgc catccgttgt cgtcgtcacc ctgcggtgcg
     1681 aggacggccg atcggagcag gtaccggcca cagacgaccg ggctcatcgt ccggtagcgg
     1741 acccaggtct cgggtgtcgc gacggtcggg tacgtgacct gcgagcccgg aatgcctgcc
     1801 ccaggcgctg ggagcgcata gccaggaccc acgctgacgc cggggccata gagctcgaac
     1861 tgggtagagt cgtagacccc gccgcggacc tcgtctccga ggccgagcgc gccggcgacc
     1921 tggttcatct cggggctgaa caggtcgaag tgcagcggcg tggcggaggg ccagccgcag
     1981 gggatggtga tctcaaggta gttgtacccc gatcccgccc cggcggtggt cgagctgtac
     2041 cagtcgccga tgccgacgcc ggcgttgggg cctgaggtct ggatgtagaa cccgccgacc
     2101 ggcgggacct cgagcgcggc gccggggctc agccatgggc cgaaggcgac ggccaccgcg
     2161 aggatggccc caccgagcag agctcggcgc aggcggcgga tgggcaggga ttttctggcg
     2221 aacacctgaa ctccatgcgg ggcacaggcc gcggagtcag cgcgcccgat gatagtcggg
     2281 ggccacgccc ggagacccgc catatccgcg cgcgcgccgt cacccgtggc cttccttgcc
     2341 cgccttcggg gccgccccta tactcaccgc ggccgccata tggcggcgta tttcttgtcc
     2401 gagggccgga gggacactcc accgagcatg acctggcgcc gtatcgcccc atgggtgatc
     2461 ctcgcgatca cgctgctggc cctgtacatc gacatcccgc gcaagtccac cggggccacc
     2521 ttcctcccgc ttccggacag catccgcacg gtgctcggcc ttgacctgca gggcggcatc
     2581 agcgtcacgc tcgaggtgca gtcggtcagt ggcgaggagc ccgaccccga gcgcgtcgag
     2641 ctggcgcgcg cgatcatcga gcgtcgagta gccggattgg gcgtgaccga gccccaggtg
     2701 cggaccgagg tcggcgcaaa cggcgaggcc cggatcgtgg tcgaggtacc cggcgtcagt
     2761 gaccggaacc agatcctcca gctcgtggga tcgacgggcc agctcctctt cctcgatcca
     2821 atggccgaga ccctgaccga gggccagaac gtcgtcgatc tcatcggcac gggcgcgatc
     2881 agtgtcctgt tcgacggcgg ggagatccag cagggaagcg tggtgccgga cacgagccag
     2941 agcggtcagg tgggcgtgag cttcaccctg acccccgagg cccaggagat ctggtgcaac
     3001 tacaccaccg gccacgtcaa ccagcccgga ccgatcgcgc tcgacggcga ggtcatcacg
     3061 accccgacga tccagcaacc gatctgcggt gggcggacga tcatcaccgt gggcgcggga
     3121 acggccgccg tcgccgagca gacgaccctc tacaaccagc ttcgtttcgg cgccttgccg
     3181 gtgtcgctca ccgtccagga agtgaccgcc atccagccca ccctgggtcg cgacttcctc
     3241 gtccaggcca tcatcgcggg cacggtcggg ctgctcctgg tcctgttctt catgatcgcg
     3301 aactaccggc tcccgggcgt gctggcttcg ctggcgctca tcttctacac gctggtggtg
     3361 tacgccatct tccggatcat cccggtcact ctgaccctgg ccggcgtcgc cgcgttcatc
     3421 ctgagcatcg gcatggcggt ggacgccaac atcctcatct tcgagcggat gaaggaagag
     3481 atccgggccg gcaagacgct cggcccggcc atcgaggccg gcttcaaccg cgcctggtcg
     3541 agcatcctcg actcgaacgt cagctccctg ctgatcgcga tctggctgtt ctggcagggg
     3601 acgagcgtga tccgtgggtt cgcgctcgtg ctcggaatcg gtgtcctggt ctcgatgttc
     3661 agtgccgtgg tcgtgacccg cactctgctc cggatggtca cccatcgcga gtggggccgg
     3721 cggatcgagt actaccacgt ggagcgctga ccgatgtacg acatcgtcgg caagcgccgc
     3781 tggtactact tcttcagcgc cctgatcacg atcccggggg tcttcttcat cctgatcggc
     3841 ggtctcaagc cgtccatcga cttcaccggt gggacggtct ggcaggtccg attcctggat
     3901 gatccatcgc cggacgaggt ccaggccgcg ctcgtggacc tcgggcacga cgaagcgacg
     3961 gtcgaggccg tggacaacgg attcctcgag atccgactgg cacccatcga gttcccgatc
     4021 gtcggcggca tcccgacccc cacgcccagc ccggcgggat cgccgaccgt gagcccttcg
     4081 ctatcggcca ccgcgactcc ttcgccggcc ggcagcccaa ccgggtcacc gggcgcatcg
     4141 ccgacggcca ccccaagccc gacgccgacg ccaaccccgt ccccaacccc gtcaccgagc
     4201 ccgacggcgg agcccacgcc cgagccgccg ccgaccatcc aggggacgcc gatcgaggag
     4261 ctggtgaccg agctcaatgc ccagttcggc ttctcggaga cgcggtcgct ggacacagtc
     4321 gggccgatca tcggcgccga gctggtgcgg agctcgctga tcatcatcat catcggcgag
     4381 gcgttcatct tcctgtacct gtggtatcgg ttccgaagca tccgct
//
LOCUS       DASYGU010000131         6997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1051416, whole genome shotgun sequence.
ACCESSION   DASYGU010000131 DASYGU010000000
VERSION     DASYGU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051416"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..759
                     /locus_tag="VGO32_03455"
     CDS             115..759
                     /locus_tag="VGO32_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEV7199840.1"
                     /translation="MCLVLMFGLSAYAWIQLPPDAQTPVHWNLSGEVDGYASKTFALL
                     FVPVLTVGLVVLLSLIPRLDPRRTNLYRSSTPYQVICSAVVVLMLVVHAGTILIALGA
                     PLDLTVVIGVGVGALLFVIGTQLGNTRSNWFMGVRTPWTLESELSWRKTHHLAGRLFM
                     AIGLISGAVAVFGPPELYFWVLLLSLLGGIVWIIRYSYVVWRDDPDRVAHRPAD"
     gene            complement(762..1301)
                     /locus_tag="VGO32_03460"
     CDS             complement(762..1301)
                     /locus_tag="VGO32_03460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV7199841.1"
                     /translation="MSERQAADIRLRDATLADADLLDAWAASPEIRGEFNDFGLGPGD
                     RSAMRETLAEGPLRNERNGELLVIRESDGAPIGTVGWHEVHYGPGPLNRAWNIGISLI
                     PEARGQGYGPIAQQLLADYLFDTTDANRVEAQTDVENAAEQRALEKAGFVREGAARGS
                     QYRAGRYHDLITYARLRSD"
     gene            1383..1919
                     /locus_tag="VGO32_03465"
     CDS             1383..1919
                     /locus_tag="VGO32_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199842.1"
                     /translation="MTRRLLAIAAMTLVIAACSGSPDASSPDDGGPGSSSGAEPSAAA
                     STGGGGSGGGAAAACDLITADEAASVMGVDSLESEPTPGDTSYCIYAGADATTLVATS
                     FNATNANDVMGAYGGQDGVTEVSGIADQALWDSNSATLFVAKGDAIVGITAGDGSMDL
                     AQRQDYSEQLGAIAAGRM"
     gene            complement(1920..3197)
                     /locus_tag="VGO32_03470"
     CDS             complement(1920..3197)
                     /locus_tag="VGO32_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199843.1"
                     /translation="MRPRSARRAYAVFLWLEAAEFYCYMLVFTASALYGIQIAGLDAL
                     QLVLVGTALEISALLFEVPTGLVADTVSRRLSIIIGCLFLAAGFILWGLVPVFAAIVA
                     AQFLWGLGATFQSGATEAWIADELQGHEVGKTYLRGSQLGYAAAALGIGSSALLGSLF
                     GLAVPMVVGGIGFLVIAATLLAFMPEDHYKPEPRGDRSRARTMADTLKAGIRTVRARP
                     VLLTVLLITFLSAGASETFDRLWEFHLLNDYTFPVIIDLPPIAWFGLISVSTLVISIG
                     VAEVARRKVDTDNHVATARALLGMDAALMVAMVVFALAVSFKMAVAGYFVARVMRRVT
                     IPISTAWINQSLTPNVRATVMSLNAQTDAVGQIAGGPLLGALAVTAGAPAALLAAAGF
                     IVPSLLLYVRTIRLHGQDVIGDEERDTPESPGR"
     gene            complement(3194..6571)
                     /gene="mfd"
                     /locus_tag="VGO32_03475"
     CDS             complement(3194..6571)
                     /gene="mfd"
                     /locus_tag="VGO32_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV7199844.1"
                     /translation="MTRGPRLGPLLSLLGPPPPPPVELRVPTAARAAHLASRRASVER
                     TRPLVVVVETDEQAHRLADELGAWLDESTVAVLPERGALPLERALPDRDESAARLTVL
                     ARLAGSERDLVIVAPLAAVWQRTLSVDRLRGARRRIRVGDRVPQRDLTNGLLAAGYDA
                     TPEVTGIGEIAVRGGLIDLWPPGEPDPIRIEQFGDEIDAIRVFDPTTQASRSRRKEIQ
                     LLPAGEFAVPAPDELRAALIAQVGEVEALSETLQTDLARLEAGDAGEAAETWITHLTA
                     APTADHLPADAHLVLTDVDQLRGHAAEADRWAADRRSHLIEDGELPPHWQPPYAAAET
                     LDSLLERSAERLEEGGATDAGFGPAPALPGRSEQIGEWLREQAGGGARVVVATDQASR
                     LAELIGDLPGRIGPSDALEAPPPPGSVALVHGSTGIGFIHQPSNLVLLSDRELFGAAR
                     VRRLLTSKRVVTRDLLAQLEPGELVVHVDHGISRYVGMATRTLEGTVREYLQLDFAGD
                     DKIFLPADQVGRITRYSGAPAPGLSKLGGREWAATKRRVRQAVIDLADDLLELYAARE
                     QADGFSYPDDTTWQRELEESFPFTETPDQARTIGEVAKDMSRRRPMDRLVAGDVGYGK
                     TEVALRAAFRVVEAGRQVAVLVPTTVLAQQHLLTFQRRLAAFPVKVEVLSRFVSKGEQ
                     QRIVGELAAGTVDVVVGTHRLLSKDVAFADLGLLVVDEEQRFGVAQKEKIKLLRREVD
                     VLTLSATPIPRTLHLSLVGIRDLSVIETAPEARLPILTRIAEDDDGLVRDAITREIDR
                     GGQVFYVHNRVETIEAASARVRRLVPRARVAIGHGQMAEGMLERVMLDFDAGLFDVLV
                     CTTIIESGLDIPNANTMLIARADTFGLAQLYQLRGRVGRSDRRAYAYLLHRRGFPLSG
                     VARRRLHAIFSASDLGAGYQIALSDLEIRGAGNILGAEQSGYMAAVGFELYTQLLAEA
                     VDLRRGRIPPPEPAAVRLELPGSAFLPDGYVGAAGAKLEIYRRFSQVKTDADAEALRA
                     HLQDRFGPIPPDVEGLFRAVSVRLAAEVADVSEVRIGDGRLTLRWRRYDRAVVTRALT
                     VAGFRPVAGSNQVRIPLVRGRDPIETTLQVLRAVPAG"
     gene            complement(6568..>6997)
                     /gene="pth"
                     /locus_tag="VGO32_03480"
     CDS             complement(6568..>6997)
                     /gene="pth"
                     /locus_tag="VGO32_03480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV7199845.1"
                     /translation="VGTVLLVKPARFMNQSGPPLRAALRNANATYETDLLVVTDDADL
                     PLGRIRLRRGGSAGGHNGLRDIIEALGTDQFARLRVGIGRNGEMVDHVLSTFSKAEDE
                     LAREAIGTAADAVESWLAEGTDAAMNAFNGVDLDEPPAGS"
BASE COUNT         1065 a         2452 c         2445 g         1035 t
ORIGIN      
        1 cgccggacgg gtgcgcccgt cccgggtcga gattcgcctt cgagtatacg cctgcgggtt
       61 cggcagtagc atcaatccgc catgcagctg cgcgcatttc acatcgtgtc cgtggtgtgt
      121 ttggtcctga tgttcgggct cagcgcgtac gcctggatcc agctccctcc cgacgcccag
      181 acccctgtcc actggaacct gtcaggcgag gtcgacggct acgcgtccaa aacgttcgcc
      241 ctcctgttcg tgcccgtgct gaccgtcggg ttggttgtcc tcctgtcgct catcccccgt
      301 ctcgatccac gtcgaaccaa cctgtatcgg tcctcgacgc cgtaccaggt catctgctcg
      361 gcggtggtgg tcctcatgct cgtggtccac gccggaacca tcctcatcgc tcttggcgcg
      421 ccgctggacc tcacggtggt catcggagtg ggcgtgggcg cactcctgtt cgtgatcggc
      481 acccagctgg ggaacacccg gtccaactgg ttcatgggcg tgcgcacgcc gtggaccctg
      541 gaaagcgagc tgtcgtggcg caagacgcat catctcgccg gtcgcctgtt catggccatc
      601 ggcctgatca gtggcgcggt cgctgtgttc ggaccgccgg agctctactt ctgggttctg
      661 ttgctcagcc tgttgggtgg aatcgtctgg atcatccgct actcgtacgt cgtgtggcgc
      721 gacgacccag atcgcgttgc gcatcgcccc gctgactagg ctcagtcgct ccgcaacctc
      781 gcgtaggtga tcaggtcgtg atagcggcct gcgcggtact gggagccgcg ggcggctcct
      841 tcgcgcacga agccggcctt ctcgagggcg cgttgctcgg ccgcgttctc gacatcggtc
      901 tgcgcttcga cccggttggc atcggtcgta tcgaacaggt agtcggccag cagctgctgg
      961 gcgatcgggc catagccctg cccgcgggcc tcggggatga gtgagatgcc gatgttccac
     1021 gcccggttca agggacccgg gccgtaatgg acctcgtgcc agccgaccgt gccgatcggc
     1081 gcaccgtcgg attcgcgaat gacgagcagc tctccgttcc gctcattgcg gagcggtccc
     1141 tcggccagcg tctcgcgcat cgcggaccga tcaccgggcc ccagcccgaa gtcattgaac
     1201 tcgccgcgga tctcgggtga ggcggcccat gcgtcgagca ggtcggcatc ggccagcgtg
     1261 gcatctcgaa gccggatgtc tgccgcctgt cgctcgctca cctcaggtcc gacctcgact
     1321 gtggatttgg agccgacgcg tattctgccc aaaagcagaa atgcgcacgg aggactcgtc
     1381 ccatgacccg cagacttctc gcgattgcgg cgatgaccct ggtcattgcc gcctgctccg
     1441 gctcgccgga cgcttcgagc cccgacgacg gtgggcccgg ttcgagcagc ggcgccgaac
     1501 cgagcgcagc ggcatccacc ggaggtggtg ggagcggtgg cggcgcggca gcggcctgcg
     1561 acctgatcac ggccgatgag gcggcatcgg tcatgggagt tgacagcctg gaaagcgagc
     1621 cgacccctgg cgacacctcg tactgcatct acgccggggc cgatgcgacg acgttggtag
     1681 cgacctcatt caacgccacg aatgcgaacg acgtcatggg cgcctacggg ggacaggacg
     1741 gcgtgaccga ggtctccgga atcgcggacc aggcgctgtg ggactcgaac tcggcaaccc
     1801 tgttcgtggc caagggggac gccatcgtgg ggatcacggc tggcgacgga tcgatggatc
     1861 tggcgcagcg ccaggattac tccgagcaac tgggggcaat cgccgccgga cgcatgtagt
     1921 catcggccgg gggactccgg cgtatcacgt tcctcgtcgc cgatgacgtc ctggccgtgg
     1981 aggcggatcg tccgcacgta gagcagcagt gacggcacga tgaaaccggc cgcggccaac
     2041 agggcggccg gggcgcccgc ggtgacggcc agtgcgccga gcagcgggcc gcccgcgatc
     2101 tggccgaccg catcggtctg agcattcagg gacatgaccg tggcccgcac gttcggggtc
     2161 aggctctggt tgatccaggc ggtgctgatc gggatcgtca cacggcgcat gacgcgcgcg
     2221 acgaagtagc cggccaccgc catcttgaag ctcaccgcca gcgcgaacac gaccatggcc
     2281 accatcagcg cggcatccat ccccaggagc gcgcgtgcgg tcgcgacgtg gttgtcggtg
     2341 tccacctttc ggcgggccac ctccgccacg ccgatgctga tcacgagggt ggagacgctg
     2401 atcagcccga accaggcgat gggcggcagg tcgatgatca cgggaaacgt gtagtcgttg
     2461 aggagatgga actcccacag ccgatcgaag gtctcgctcg caccggcgct cagaaacgtg
     2521 atcagcagga cggtcagcag aacaggacgg gcccgcacgg tccgaatgcc ggccttcagc
     2581 gtgtcggcca tggttcgggc ccggctgcgg tcgccgcgcg gctccggctt gtagtggtcc
     2641 tcgggcatga acgcgagcag cgtcgcggcg atcacgagga agccgattcc gcccaccacc
     2701 atgggcaccg ccaggccgaa cagcgacccg aggagcgcgc tgctgccgat ccccagcgcc
     2761 gccgcggcat agccgagctg cgaaccacgc aggtaggtct tccccacctc gtgaccctgg
     2821 agctcgtccg cgatccacgc ctcggtcgca ccggactgga acgtcgcgcc cagtccccac
     2881 aggaactgcg cggcgacgat ggcggcgaac accgggacga ggccccacag gatgaatccg
     2941 gccgcgagga acaggcagcc gatgatgatc gagagccggc ggctgacggt gtcagccacc
     3001 agcccggtgg gcacctcgaa caggagggcc gatatctcga gcgcggtccc gaccaggacc
     3061 agctgaagcg catcgagtcc agcgatctgg atgccgtaca gcgcgctggc cgtgaaaacc
     3121 agcatgtagc agtagaactc ggccgcctcg agccagagga agacggcgta cgcgcgccgc
     3181 gcggaccggg gcctcacccg gcaggcaccg cccgcaggac ctggagcgtg gtctcgatcg
     3241 ggtcgcggcc acggaccagc ggaatgcgga cctggttgga gccggccacc gggcggaacc
     3301 cggcgacggt cagggcgcgg gtcaccaccg cccggtcgta acgacgccac ctcagggtga
     3361 gccgcccgtc accgatccgg acctcggaca cgtcggccac ctccgccgcc aaccgcacgc
     3421 tgaccgcccg gaacaggccc tcgacgtcgg gcgggatcgg tccgaaccgg tcctgcaggt
     3481 gggcgcggag cgcctcggca tcggcatcgg tcttgacctg ggagaaccgc cgatagattt
     3541 cgagcttggc accggcggca cccacgtacc cgtcgggcag gaacgcagac cccggcagct
     3601 cgagccggac cgccgccggc tccggtggcg ggatccggcc gcggcggagg tccaccgcct
     3661 cggccaggag ctgggtgtag agctcgaatc caaccgccgc catgtagccc gactgctccg
     3721 cacccaggat gttgccggcg ccccggatct cgagatcgga cagcgcgatc tggtatcccg
     3781 ctccgaggtc cgaggcgctg aagatcgcgt gcagccgtcg acgagcgacg ccggacagcg
     3841 ggaagccgcg ccggtggagg aggtacgcgt acgcccggcg gtcggagcgg ccaacccggc
     3901 cgcgcagctg atacagctgg gccagcccga acgtgtcggc ccgggcgatg agcatggtgt
     3961 tcgcgttcgg gatgtccagg ccggactcga tgatcgtggt gcacaccagc acgtcgaaca
     4021 ggcccgcgtc gaagtcgagc atgacccgct cgagcatgcc ctccgccatc tggccatgac
     4081 cgatggccac ccgcgccctg gggacgagcc ggcgaacccg ggcggaggcg gcctcgatgg
     4141 tctcgacccg gttgtggacg taaaagacct gcccgccgcg atcgatctcg cgcgtgatcg
     4201 cgtcgcggac cagtccgtcg tcgtcctccg cgatgcgagt caggatcggc agccgcgcct
     4261 cgggtgcggt ctcgatgacg ctcaggtcac ggatcccgac caggctcagg tggagcgtgc
     4321 gcgggatggg cgtggcagac agggtcagga cgtccacctc gcggcggagg agcttgatct
     4381 tctccttctg cgccaccccg aaccgctgct cctcgtccac cacgagcagc ccgaggtcgg
     4441 cgaacgccac gtccttgctc aagagccgat gcgtgccgac cacgacatcc accgtccccg
     4501 ccgccagctc acccacgatc cgctgctgct cgcccttgga cacgaagcgg ctgagcacct
     4561 cgaccttgac cgggaaggcg gccagccggc gctgaaaggt gagcaggtgc tgctgggcga
     4621 ggaccgtggt cgggaccagg accgccacct ggcgtccggc ctccaccacc cggaacgcgg
     4681 cacggagggc gacctcggtc ttgccgtagc ccacgtcgcc ggccaccaac cggtccatcg
     4741 gccgccgacg ggacatgtcc ttcgcgacct cgccgatcgt ccgcgcctgg tcgggcgtct
     4801 cggtgaacgg gaacgactcc tccagctccc gctgccaggt cgtgtcgtcg gggtacgaga
     4861 agccatcggc ctgctcgcga gcggcgtaca gctcgagcag gtcgtcggcg aggtctatca
     4921 ccgcctggcg gacccgccgc ttggtggcgg cccactcccg tccgccgagc ttggacaggc
     4981 cgggcgccgg cgcgccggaa tagcgcgtga tcctccccac ctggtcggcg ggcaggaaga
     5041 tcttgtcgtc gccggcgaag tccagctgga ggtactcgcg gacggtgccc tccagcgtcc
     5101 gggtggccat tcccacgtaa cgcgagatcc cgtggtcgac gtggacgacc agctcccccg
     5161 gctcgagctg ggcgagcagg tcgcgggtga cgacccgctt ggaggtgagc agccgacgca
     5221 cgcgggcggc accgaacagc tcccgatcgg agagaaggac gaggtttgaa ggctggtgga
     5281 tgaagccgat cccggtcgag ccatggacca gcgccacgct gccgggaggc ggcggcgcct
     5341 cgagcgcgtc ggatggaccg atccggcctg gcaggtcgcc gatcagctcg gccagccggc
     5401 tggcctggtc ggtggcgacc acgacccggg ccccgccacc ggcctgctcg cggagccact
     5461 ccccgatctg ctcgctgcgt cccgggagcg ccggcgcggg cccgaatccc gcgtcggtgg
     5521 cgccaccttc ctccagccgc tccgcgcttc gttcgaggag ggagtcgagc gtctcggcgg
     5581 cggcgtacgg cggctgccag tgcggaggca actcgccgtc ctcgatcagg tgggaccgcc
     5641 gatcggcggc ccagcggtcc gcctccgccg catggccgcg gagctgatcg acatcagtca
     5701 ggacgaggtg cgcgtcggcg ggcaggtgat ccgcggtggg ggcggcggtc aggtgggtga
     5761 tccacgtctc cgcggcctca cccgcgtcgc ctgcctcgag gcgggcaaga tcggtctgga
     5821 gcgtctcgct cagcgcctcg acctccccca cctgcgcaat gagcgcggcc cgcagctcgt
     5881 cgggggcggg caccgcgaac tcgcccgcgg gcagcagctg gatctccttc cggcgggagc
     5941 gacttgcctg ggtcgtcggg tcgaacacgc ggatggcgtc gatctcgtcg ccgaactgct
     6001 cgatccggat cgggtccggc tcgcccggcg gccacaggtc gatcaagccg ccgcggacgg
     6061 cgatctcgcc gatgccggtc acctcgggcg tggcgtcata tccggctgcg agcagcccgt
     6121 ttgtgagatc ccgctgcggg acgcggtcgc cgacccgtat gcgccggcga gcaccgcgca
     6181 gccgatccac ggacagggtc cgctgccata cggcggcgag cggggcgacg atcaccaggt
     6241 cccgctcgga acccgccagc cgggccagga ccgtgagccg cgcagcggat tcgtcccgat
     6301 cggggagcgc ccgttccaac ggcagggcgc cgcgctcggg cagcacggcc accgtgctct
     6361 cgtccagcca tgccccgagc tcgtcggcca ggcgatgggc ctgctcatcg gtctcgacca
     6421 cgaccacgag cggccgggtt cgctcgacgc ttgcgcgacg cgaggcgaga tgcgccgccc
     6481 gcgcggcggt cggtacgcgc agctcgaccg gcggcggagg cggcgggccc aggaggctga
     6541 gaagcggccc gagccgcgga ccgcgggtca cgacccggcg ggcggttcgt ccaggtcgac
     6601 cccgttgaac gcgttcatcg cggcgtcggt cccctcggcc agccacgact cgaccgcgtc
     6661 ggcggcggtg ccgatcgctt cgcgcgcgag ctcgtcttcg gccttgctga aggtggacag
     6721 gacgtgatcc accatctcgc cattgcgccc gatcccgacc cgcagccgcg cgaactggtc
     6781 ggtgccgagg gcctcgatga tgtcgcgcag cccgttgtgg ccgccggccg agcctccgcg
     6841 tcgcagccgg atgcgaccca ggggcaggtc cgcgtcatcg gtcaccacca gcaggtcggt
     6901 ctcgtaggtc gcgttcgcgt tgcgcagggc cgcgcggagt ggcggccccg actggttcat
     6961 gaaccgcgcc ggcttgacga ggaggacggt gcccacc
//
LOCUS       DASYGU010000132         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1617219, whole genome shotgun sequence.
ACCESSION   DASYGU010000132 DASYGU010000000
VERSION     DASYGU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617219"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VGO32_03485"
     CDS             complement(<1..939)
                     /locus_tag="VGO32_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEV7199846.1"
                     /translation="MLDRRPILMLIDGPSLVYRGYFALPPLTTSDGTLVNAAFGFLQI
                     VLRGMADMKPDYAVVTFDLGKPAFRFIAYPDYKAGRPSMPDDLRSQFPIVREVVTGLG
                     MPIVEIEGYEADDVIGTLVQHATAADVDTVIVSGDLDGLQLVNDHVRLLTTRMGVAST
                     VVYDPQRVMDRYGLRPDQMLDYKALKGDTSDNLPGVPGVGEKTAIALLQEFGTLDSMY
                     ERVDEVKGKLRERLVEHREEALMSREVGRIVTDLPVTLDLEAARTGRYDRRALAQRFR
                     ELEFRSLIDRLPPTIGSSAGPDEEEDRGGGLQLSLDL"
     gene            1010..1513
                     /locus_tag="VGO32_03490"
     CDS             1010..1513
                     /locus_tag="VGO32_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV7199847.1"
                     /translation="MPSGTVRDPYAVLGVPREATTRQIAAAHRKLAKSHHPDLHDGGP
                     EAAVRMREINEAWTLLSSPERRVAWDIEHPWSGTAAAGSHWAGGRQTIPAGSTVRAQV
                     STPWRTAANDPRSARRGRIRVSPRPPRVEAPPKTFLEGPWAVIAAGGVALVILTAAII
                     AGRLIGA"
     gene            complement(1543..2232)
                     /locus_tag="VGO32_03495"
     CDS             complement(1543..2232)
                     /locus_tag="VGO32_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199848.1"
                     /translation="MTMMSPPHPDPERLAALASQDADVVADRALTDHVASCAECTLQV
                     REISALRATLAELPDLTPSRPMQLVPPVAAPPVAAGWRTVFRRAFAPVAVAGMVLLLV
                     GGIGATGVLGPADAQRLVFLPFQAATGGSPAERPVTEEAAPGVTATDGGVVAPAASAP
                     ADNAGSGGGEDDPGTSRGEGEPGSPAAEDLDSSGGSVTGWVILAMAGLALLVLAFFLR
                     IAAPTRSAAKR"
     gene            complement(2246..2857)
                     /locus_tag="VGO32_03500"
     CDS             complement(2246..2857)
                     /locus_tag="VGO32_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7199849.1"
                     /translation="MPDPDPSRPTTRTGPLADEAAVVAAAVGGDRSAFARLMEHYQGA
                     CYGLAWRLLGDPDQAADATQDAFIHAYDHLASYRGGAFRSWLLRITANASYDILRRAQ
                     RRPTTALPEPAEGEADIPDPVAVNPVVESARSELYRALDVALRLLPEDQRTAVILCDV
                     YGMDYDEVSLTTSVPVGTVKSRIHRGRLRLRVLLADQRELFDA"
BASE COUNT          446 a          999 c         1076 g          395 t
ORIGIN      
        1 gaggtcgagc gacagctgca gaccaccgcc tcgatcctct tcctcgtcgg ggcctgccga
       61 gctgccgatg gtcggcggca accggtcgat gagcgagcgg aactcgagct cgcggaagcg
      121 ctgggccagc gcacgccgat cgtagcggcc ggtgcgggcg gcctccagat ccagggtcac
      181 cggcaggtcg gtcacgatcc ggccgacctc gcgggacatg agcgcctcct cgcggtgttc
      241 gaccagccgc tcccgtagct tgcccttgac ctcgtccact cgctcgtaca tcgaatcgag
      301 cgtgccgaac tcctggagca gggcgatcgc ggtcttctcg ccgaccccgg gcacgcccgg
      361 caggttgtcc gacgtgtcgc ccttgagcgc cttgtagtcg agcatctggt ctggccggag
      421 tccgtatcgg tccatgaccc gctgcgggtc gtagacgacc gtggaggcca cccccatccg
      481 ggtggtgagc agccggacgt ggtcgttcac gagctggagg ccatccaggt cgccggagac
      541 gatgaccgtg tccacgtccg ccgcggtggc gtgctggacg agcgtgccga tgacgtcgtc
      601 ggcctcgtag ccttcgatct cgacgatggg catgcccagg ccggtgacca cctcgcgcac
      661 gatcgggaac tgggagcgca ggtcatcggg catggacggc cggccggcct tgtagtcggg
      721 ataggcgatg aaccggaatg cgggcttgcc caggtcgaac gtgacgacgg cgtagtcggg
      781 cttcatgtcg gccatgccgc ggagcacgat ctgaaggaat ccgaacgccg cgttgaccag
      841 cgtgccgtcc gaggtcgtga gcggcggaag ggcaaaatag ccgcgataca cgaggcttgg
      901 accgtcgatg agcatcagga tcgggcgacg gtcgagcatg aagggagtct agtccgcaag
      961 gaccgatgag cggccgatga gccgccgggc cgatcgctag aatccacgga tgccaagcgg
     1021 aacggtgcgc gacccctatg cggtgctcgg cgtcccccgc gaggccacca cccgccagat
     1081 cgcggccgcc catcggaagc tggcgaagtc ccaccatccc gacctccacg acggaggccc
     1141 cgaggcggcg gtgcggatgc gcgagatcaa cgaggcgtgg acgctcctgt cgtcgcccga
     1201 gcgtcgagtg gcgtgggaca tcgagcatcc ctggagcggg acggcggcgg caggcagcca
     1261 ctgggcaggc gggcgacaga cgattcccgc cggctcgacg gtccgcgccc aggtctcgac
     1321 cccatggcgg accgcggcga acgacccgcg cagcgctcga cgtggccgta tccgggtcag
     1381 tccgcggcca cctcgcgtcg aggccccacc gaagacattc ctggagggtc cgtgggcggt
     1441 catcgcggcc ggcggcgtgg ccctcgtgat cctcactgcc gcgatcatcg cggggcggtt
     1501 gatcggggcc tgacccagcg cctgcgggcc gggctggaaa tctcagcgct tggccgcgct
     1561 ccgcgtcggg gcggcgatcc gcaggaagaa ggccaggacc aggagggcga ggccggccat
     1621 cgccaggatc acccagccgg tcacgcttcc gccgctcgaa tcgaggtcct cagcggccgg
     1681 cgatccgggc tccccttccc cgcgcgacgt gccagggtcg tcttcgccgc cacccgatcc
     1741 cgcattgtca gccggggctg acgccgccgg agcgaccacg ccgccgtcag tcgcggtgac
     1801 acccggcgcc gcttcctcgg ttaccgggcg ctcggccgga ctgccgccgg tggcggcctg
     1861 gaacggcagg aagaccagtc gctgggcatc cgccgggccc agcacgccgg tcgcgccgat
     1921 gccgccgacc aggaggagca ccatgccggc gaccgcgacc ggcgcaaacg ctcgacggaa
     1981 taccgttcgc cagcccgcgg cgaccggtgg agcggcgacg ggcggaacga gctgcatggg
     2041 ccgggacggg gtgaggtccg gcagctcggc gagcgtcgcc cgaagggcgg agatctcccg
     2101 aacctggagc gtgcattcgg cgcagctcgc cacgtggtcg gtgagcgcac gatccgcgac
     2161 gacatcggcg tcctggctgg ccagggcggc gaggcgctcg gggtcgggat ggggaggcga
     2221 catcatggtc atcggtcaga cgccctcacg catcgaacag ttcccgctgg tcggcgagca
     2281 gaacgcgcag ccgaagccgt ccacggtgaa tgcgcgactt caccgtgccc accggcaccg
     2341 aggtcgtcaa cgaaacctcg tcgtagtcca tcccgtacac gtcgcacagg atgaccgccg
     2401 tgcgctggtc ctcggggagg agccggagcg cgacgtccag ggcgcggtag agctccgagc
     2461 gcgccgactc cacgaccggg ttgacggcca ccgggtcggg gatgtcggcc tcgccctcgg
     2521 ccggctccgg gagcgcggtg gtcggccgtc gctgggcgcg gcggaggatg tcgtacgacg
     2581 cgttggccgt gatccggagc agccaggatc ggaacgcgcc gccgcggtac gaggcgaggt
     2641 ggtcgtaggc atggatgaac gcgtcctggg tggcgtccgc ggcctggtcc gggtcgccga
     2701 gcaggcgcca ggcgaggccg tagcaggcgc cctgatagtg ctccatcagg cgggcgaagg
     2761 ccgaccgatc gccgccgacg gcagcagcca ccactgcggc ctcgtcggcc agcgggccgg
     2821 tccgcgtggt cgggcggctc gggtcggggt ccggcatcgg cttcctataa tcacaccccg
     2881 cacggccgga gtggcggaac tggcagacgc gtcggt
//
LOCUS       DASYGU010000133         4328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1257194, whole genome shotgun sequence.
ACCESSION   DASYGU010000133 DASYGU010000000
VERSION     DASYGU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257194"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..584)
                     /locus_tag="VGO32_03505"
     CDS             complement(129..584)
                     /locus_tag="VGO32_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199850.1"
                     /translation="MTRVLVVHHDLDVSDIEVDLLRRAGYEVDQCQGPVGGSPCPVLH
                     GERCWQVEKADVLVYDAWLTGDGAETLVDDLRDLHPDKPVVVTSPGMMASWAEAGGRH
                     GVTRADWAAQGGLAGAIEDAIERGPEAPSSPEPGKSKAEETPPKPGPHW"
     gene            complement(687..2165)
                     /locus_tag="VGO32_03510"
     CDS             complement(687..2165)
                     /locus_tag="VGO32_03510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7199851.1"
                     /translation="MELFATMDRPWPLPRPGVRRAWWLREALATEQVTGGTLPAPPLT
                     GHEIADVAIVGGGYTGMWTAYFLTERDPGMRIVLLEQDICGGGPSGRNGGFVHGWWEQ
                     LPYLVKKYGPEAGLEIAKAADEVVDGIGAFCTEHGIDAWYRKAGYLAAHAFPNVESGF
                     ERSSAAVARLGGHGQLVTWDPARVQAVCASPVFRDGQWMPSAASIQPARLARGLRRVL
                     IERGVVIHENTRVRRINPHSGSGGPGQPSVGVITDDGSVLAHEAVLAVNAWGAGWPGH
                     RLRLLAWGSYMIVTEPIPDRLAALGWTGDELLSDTRFTISYFRTTRDGRIAFGAGVGA
                     AGFGGRIGKTFSSDAHAVARVASNFDHLFPMLRDVRFEDSWGGPIDISADRFPEIGSR
                     DGRRVHYAYGFAGNGAGPSRLAGRILAALVDDPNDPLARLPLVGRRQRILPPEPFRFI
                     GARLIREALIRRDDALDAGETPGLVVRTMARVPGLMGYRFEH"
     gene            2238..2975
                     /locus_tag="VGO32_03515"
     CDS             2238..2975
                     /locus_tag="VGO32_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7199852.1"
                     /translation="MRLTVIGGSGGYPGRGRPCSGYLVEADGFALLIDPGYGVATALS
                     IDDRLRFDAVLVSHAHPDHCADLNPILRARAWADVLLSPLPVHALPGALDAVLALDRP
                     EVLAGSYVLRDFEPDGELSIGPFQVLTAPLPHPRPNLGFRISVGGRSLVYTGDCGPSD
                     GLIHLADGANLLLAEASYAETVPPELVGSLSSAADVGREAATGGVQRLVLTHLVPKTN
                     EADAVAAAARGFAGPIDVARPGLVVEA"
     gene            3057..4289
                     /locus_tag="VGO32_03520"
     CDS             3057..4289
                     /locus_tag="VGO32_03520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7199853.1"
                     /translation="MTTNGDPARTAVVVDGLRTPFGRYGGALAHARPDDLAAHVIKAV
                     VERTGIDPASIDDVIIGAANQAGEDNRNVGRMAVLLAGLPTSVPGQTVNRLCGSGMSA
                     IVAAAHAIAAGDGSVFVAGGTESMTRAPLVMAKPSAAFPRGEQTIYDTTLGWRFTNPR
                     LAEAHYPYSMGETAENVVEKIGVSRQDQDRFALESHRRWAVAHEAGRFADEVIPISVP
                     GAKKGETVTVDRDEHPRPDTTLEQLAGLKPAFRKDASGSVTAGNSSGINDGAAAVVVM
                     GEERAKAAGLRPILRMRSSAVAGVDPATMGLGPIPASRLALERAGLSVGDLDLVELNE
                     AFAAQALPVMRELGLDPEKVNVNGGAIAIGHPLGASGARLVATLAHEMRRRGARYGLA
                     TMCIGVGQGIATVWETAE"
BASE COUNT          634 a         1559 c         1495 g          640 t
ORIGIN      
        1 cagcgcggga cggcggttcc gatgggtgcg ggcgagcacg gccaaacctc ctcctcatcc
       61 ggccgccgag gagatctcgc ggtcgttcga ccgatgctac gccgccgctt tccggcgggt
      121 caacggttct accagtgcgg accgggtttg ggtggcgttt cctccgcctt cgacttgcct
      181 ggctcggggc tggatggcgc ctcgggcccg cgctcgatgg cgtcctcgat cgcgccagcc
      241 aggccgccct gtgcggccca gtccgcccgc gtcaccccgt gtcttccacc cgcctctgcc
      301 cagctggcca tcatccccgg ggaggtgacg accaccggct tgtcagggtg gagatctctc
      361 aggtcatcga ccagggtttc cgccccatcc ccggtcagcc aggcgtcata gacgagcacg
      421 tccgccttct ccacctgcca gcagcgctcg ccgtggagaa cggggcacgg cgatccgccg
      481 actggcccct ggcattggtc gacctcgtag cccgctcgac gcagcaggtc gacctcgatg
      541 tccgacacgt caaggtcatg gtggacgaca agtacgcggg tcatgcctga actccttggc
      601 cggatgattg gcctctcctg ccaggaagga cagtgcccga tggccccttt ctcgcggccg
      661 aacggcctga gacgggagac gggagctcag tgctcgaagc ggtagcccat gagccccggc
      721 acccgcgcca tggttcgaac cacgagcccg ggcgtctctc cggcgtccag cgcgtcatcg
      781 cgccggatga gcgcctcgcg gatgagccgt gcgccgatga accggaacgg ctcgggcggg
      841 aggatccgct gccggcgccc gacgagcggg agccgggcca gcggatcgtt cgggtcgtcc
      901 accagcgcgg ccaggattcg ccccgccagg cgcgacgggc cggccccgtt tccggcgaag
      961 ccgtacgcgt agtgaacccg ccggccatct cgtgatccga tctccgggaa ccggtcggcg
     1021 ctgatgtcga tcgggccgcc ccaggagtct tcgaaccgga cgtctcgaag catggggaac
     1081 aggtggtcga agttggacgc gacccgggcc acggcatgtg catctgagct gaacgtcttc
     1141 ccgatccgcc cgccgaagcc cgccgccccg accccggccc cgaacgcgat ccgcccgtca
     1201 cgcgtggtcc ggaagtagct gatcgtgaac cgggtatcac tgagcagctc gtccccggtc
     1261 cagcccagcg cggccagccg atcggggatg ggctcggtca cgatcatgta gctgccccag
     1321 gccagcagcc ggagccgatg gccgggccag ccggctcccc acgcgttcac cgcgaggacc
     1381 gcctcgtggg ccaggactga tccgtcgtcg gtgatgacgc ccacgctcgg ctggcccggg
     1441 ccaccgctgc ccgagtgggg gttgatccgg cggacgcggg tgttctcgtg gatcaccacg
     1501 ccgcgttcga tcaggacgcg acgaagtccg cgcgccagcc gggccggctg gatcgaggct
     1561 gcgctgggca tccattggcc gtcccggaaa accggggacg cgcagaccgc ctggacccgc
     1621 gccggatccc aggtgacgag ctgaccatgg ccgcccaggc gagcgaccgc cgccgaggat
     1681 cgctcgaagc cgctctcgac gttgggaaat gcgtgcgccg cgaggtagcc ggccttccga
     1741 taccacgcgt cgatcccgtg ctcggtgcag aacgccccga tcccgtcgac cacctcgtcg
     1801 gcggccttcg cgatctcgag ccccgcctcg gggccgtact tcttgacgag gtacgggagc
     1861 tgctcccacc agccgtggac gaagccgccg ttccgcccgg acggcccgcc gccacagatg
     1921 tcctgctcca ggagcacgat ccgcatgccc gggtcccgct cggtcaggaa gtaggcggtc
     1981 cacatcccgg tgtacccgcc accgacgatc gccacgtcgg cgatctcgtg tcccgtgagc
     2041 gggggagcgg gcaacgtgcc gccggtcact tgctcggtag cgagcgcctc gcggagccac
     2101 cacgcccgcc tgacccctgg ccgcggcagc ggccagggtc ggtccatcgt cgcgaacaac
     2161 tccacccgcc gatcgtagtc ggtgcgccgg gtcgtggctc caattggagg agccggcgcg
     2221 ctcgctatcc ttcggcgatg cggctgacag tgatcggggg cagcggcggg tatccgggtc
     2281 gcggccgccc ctgcagcggc tacctcgtcg aagccgatgg cttcgccctc ctcatcgacc
     2341 ccggttacgg ggtcgccacg gcgctctcga tcgatgatcg gcttaggttc gacgcggtgc
     2401 tcgtcagcca tgcccaccct gaccactgcg ccgacctcaa tccgattctc cgagcccgcg
     2461 cgtgggcgga cgttctcctg tcgccgctcc ccgtccatgc actgccagga gcactcgacg
     2521 ccgtcctggc tctcgatcga cccgaggtgc tggccggctc atacgtgctc cgcgacttcg
     2581 agcccgacgg cgagctgagc atcgggccgt tccaggtcct caccgcaccg cttccccacc
     2641 cgcggcccaa tctcgggttc cgcatcagcg tcggcgggcg atccctggtg tacaccggcg
     2701 actgcggccc ctccgacggg ctgatccacc ttgccgatgg cgccaacctc ctgttggcgg
     2761 aggcctcgta cgccgagacc gtcccgccgg agctcgtcgg ctcgctctcg agcgcggcag
     2821 acgtcggtcg cgaggctgca acgggcggag tccaacgcct ggtactcact cacctcgtgc
     2881 cgaagaccaa cgaggccgat gcggtcgccg ccgcggcgcg cggtttcgcc ggtcccatcg
     2941 acgtggcgcg cccgggtctc gtcgtggagg cttgaccggc ccaatcggag ggcagaccag
     3001 gccagtcccg gttgagccgt attagcctga cgttcgggta gcggcatact gtcgaagtga
     3061 cgacgaacgg agatccggcc cggaccgcgg tcgtggtcga cggcctgagg acgcccttcg
     3121 ggcggtatgg cggagcactg gcacacgccc gtccggacga cctggcggcc cacgtcatca
     3181 aggccgtggt cgagcggacc ggcatcgatc cggcgtccat cgacgatgtg atcatcggcg
     3241 ccgccaacca ggccggtgaa gacaaccgca acgtgggccg gatggccgtc ctgctggccg
     3301 gcctgccgac atcggtcccg ggccagaccg tgaaccggct ctgcgggagc gggatgtccg
     3361 cgatcgtggc cgccgcgcac gccatcgcgg ccggtgacgg ctcagtgttc gtggccggcg
     3421 gcaccgagtc gatgacccgc gcgcccctgg tcatggccaa gccctccgcc gcgttcccgc
     3481 gcggcgagca gacgatctac gacaccaccc tgggctggcg gttcacgaac ccccggctgg
     3541 cggaggccca ctatccgtat tcgatgggcg agacggcgga gaacgtggtc gagaagatcg
     3601 gcgtcagccg gcaggaccag gaccgattcg cgctcgagtc gcaccgccgg tgggcggtcg
     3661 cgcacgaggc cgggcggttt gcggacgaag tcatcccgat ctcggtgccg ggggcgaaga
     3721 agggcgagac cgtgaccgtg gaccgcgacg agcacccacg accggacacc accctggagc
     3781 agctcgccgg cctgaagccg gccttccgca aggacgcttc gggatcggtc accgccggaa
     3841 acagcagcgg gatcaacgac ggggccgcgg cggtggtggt catgggcgag gaacgggcta
     3901 aggcggccgg cctgcggccg atcctccgga tgcgctcatc ggcggtggct ggggtggatc
     3961 cggcgacgat gggtctcggc cccatcccgg cttcgcgcct cgcgctcgaa cgggccggcc
     4021 tgtcggtcgg cgacctggac ctcgtggagc tgaacgaggc cttcgccgcc caggcactcc
     4081 ccgtcatgcg cgagctgggg cttgatccgg agaaggtcaa cgtcaacggt ggggccatcg
     4141 ccatcggtca tccgctcgga gcatccggcg cccgcctggt ggcgaccctt gcccacgaga
     4201 tgcgccgccg cggggcgcgg tacgggctgg ccaccatgtg catcggcgtc ggccagggga
     4261 tcgcgacggt ctgggaaacc gcggaatgaa ggagacgaca tgaaggacca gatgcgtcgg
     4321 gcgatcgt
//
LOCUS       DASYGU010000134         5482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1102614, whole genome shotgun sequence.
ACCESSION   DASYGU010000134 DASYGU010000000
VERSION     DASYGU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102614"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VGO32_03525"
     CDS             <1..888
                     /locus_tag="VGO32_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7199854.1"
                     /translation="MGYDPSVPPEVPLERRSGRLVEQSTDGRRRLTEANLRLVVSIAK
                     KYIGRGMSFLDLIQEGNIGLIRAVEKFEYEKGFKFSTYATWWIRQAITRAIADQARTI
                     RIPVHMVETINRLIRVSRTLLQELGREPTVEEIARRMSRDEIVRELRDKLEREPTEEE
                     VDKREAAGPQTVSPEKVREIMKVSQEPVSLETPIGEEEDSHLGDFIPDLASVAPADAA
                     SHQLLKEQVEGVLDSLTPRERRVLQLRFGLEDGRSRTLEEVGRDFNVTRERIRQIEAK
                     ALRKLRHPSRSRKLKDYLE"
     gene            922..1104
                     /locus_tag="VGO32_03530"
     CDS             922..1104
                     /locus_tag="VGO32_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199855.1"
                     /translation="MCLTCGCMQAHLDMGEHNIRYEDIEAAADENGRTVAETLDIMER
                     TKAIDREDHPTEYEPA"
     gene            1143..1217
                     /locus_tag="VGO32_03535"
     tRNA            1143..1217
                     /locus_tag="VGO32_03535"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1175..1177,aa:Asn,seq:gtt)
     gene            1326..1610
                     /locus_tag="VGO32_03540"
                     /pseudo
     CDS             1326..1610
                     /locus_tag="VGO32_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1771..1845
                     /locus_tag="VGO32_03545"
     tRNA            1771..1845
                     /locus_tag="VGO32_03545"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1803..1805,aa:Asn,seq:gtt)
     gene            1955..3205
                     /locus_tag="VGO32_03550"
     CDS             1955..3205
                     /locus_tag="VGO32_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199856.1"
                     /translation="MNELARGVQRTYLILTLLTTLATSFIWGINTLFLLDAGLSNTEA
                     FTANAFFTLGQVIFEVPTGVVADVRGRRFSFLLGAGTLLLSTLLYLVMWNIEAPFWGW
                     AVASALLGLGFTFFSGATEAWLVDALHASSFTGTLESVFARAQIVGGVAMLSGSVLGG
                     FVAQLTNLGVPYILRALMLGVTLVVAWWFMKDIGFTPQRGASPIGAVKNVVSGAVDGG
                     LRNPPVRWLMLAAPFSAGVGIYAFYAMQPYLLQLYGDPTAYGVAGIAAAMVAGAQILG
                     GTLVPWFRRFFRLRTDALILGAVVSSLLLVLIGLVTSFVLALVLLAIWCISLALVSPL
                     RQAFINGVIPSEQRATVLSFDSLMGSAGGVVAQPILGRSADVFGYATSYVIAGIISAA
                     VIPFSFLARREHAPSDPIKAEEPG"
     gene            complement(3214..4077)
                     /locus_tag="VGO32_03555"
     CDS             complement(3214..4077)
                     /locus_tag="VGO32_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199857.1"
                     /translation="MAGSEAVFVGETVRAVVLPNRGARLHRLQVLGHDLIRTPPDLAA
                     YDREPFFWGSFVMAPWCNRIGTDPVTVGGRPIQLGSNFADGTAIHGEVLDRQWEILPD
                     GAFRVEAGGDGWPWRYESTLQIAVAEHHLRVDIGLRNLSDDPMPAGLGIHPWFRRPVR
                     VAIRAAAVFSPNADTPPDPRPVEGSLDLRRLQTMRPGLDATWTDLASPPVELAWPQLG
                     IGATMRVTAPSLFIVAASPPGVDAIAVEPQTHAPQGLRRLLQGETGALALLPPGEAMS
                     LKTELEFRTIG"
     gene            complement(4088..>5482)
                     /locus_tag="VGO32_03560"
     CDS             complement(4088..>5482)
                     /locus_tag="VGO32_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199858.1"
                     /translation="NPAVSHHRRARMAELSLPAVGASPSTPGIPTELYEQRGGRLRAR
                     ADEAGFDRLVVYADREHSANLAYLTGFDPRFEEAVLVIGPDDPPAIMVGNECFGLAGA
                     APLTMRRQLIQDLSLPSQPRDRSRPWAELLAAEGIGPGSRVGVIGWKTYADPAASDAP
                     SYLVDELRRATGPTGSVVNATDLLIHPGNGLRVINEVEQLAAFEHAACHTSNAVRRLL
                     FGLRPGMTEHQAVSLLEWNGAPLSCHLMLTSGPRATLGLLSPGDRTVEHGDRFTVAYG
                     IWGALNCRAGFIVEGEQWLPAGIRDYVERLVGPYFAAVAEWYEALHIGQTGGALQDII
                     DRRLGDPFFGITLNPGHQIHLDEWVNSPVARSSSVELRSGMALQVDIIPATGTDYFTT
                     NIEDGIALADAALRETFATRYPDAWSRIGARRQFMRDALGIELHPDVLPFSNMPAYLP
                     PFLLRPERAMTMAG"
BASE COUNT          862 a         1868 c         1862 g          890 t
ORIGIN      
        1 atgggctacg acccgtctgt cccgcccgag gtgccgctcg agcgccggtc cggacgcctg
       61 gtcgagcaga gcaccgatgg ccgacgccgg ctgaccgagg ccaaccttcg gctggtggtc
      121 agcatcgcga agaagtacat cgggcggggg atgagcttcc ttgacctcat ccaggagggg
      181 aatatcgggc tcatccgggc ggtcgagaag ttcgagtacg agaagggctt caagttctcg
      241 acctacgcca cgtggtggat ccggcaggcc atcacccggg ccatcgccga ccaggcccgc
      301 acgatccgga tcccggtcca catggtggag accatcaacc gcctgatccg ggtcagccgg
      361 accctgctcc aggagctcgg tcgcgagcca acggtggagg agatcgcacg gcggatgagc
      421 cgcgacgaga tcgtccgcga actgcgcgac aagctcgagc gcgagcccac cgaggaagag
      481 gtggacaagc gcgaggccgc tggcccgcag acggtcagcc cggagaaggt tcgcgagatc
      541 atgaaggtca gccaggagcc ggtcagcctc gagacgccga tcggcgagga ggaggactcc
      601 cacctgggtg acttcatccc ggacctcgcg tccgtcgcgc cggccgatgc cgcctcgcat
      661 cagctcctca aggagcaggt cgagggcgtc ttggactcgc tcacgccgcg tgagcgacgg
      721 gtcctccagc tgcgcttcgg gctcgaggac ggccggtcac ggaccctcga agaggtgggt
      781 cgcgacttca acgtgacccg cgagcggatc cggcagatcg aggccaaggc tctccgcaag
      841 ctccggcacc cgagccgcag ccgcaagctg aaggactatc tggagtagac cgatacccgg
      901 ggcattggag gacattcgac catgtgtttg acctgcggct gtatgcaggc ccacctcgac
      961 atgggcgagc acaacatccg atacgaggac atcgaagcgg cggccgatga gaacggccgt
     1021 accgtggccg agacgctcga catcatggag cggaccaagg ccattgaccg agaggatcac
     1081 cctacggagt acgaaccggc ctgacggggt ctcaccgggg aggggcctat aatccgccgc
     1141 gttccggcgt agctcagtgg tagagcagac ggctgttaac cgtcgggtcg taggttcgaa
     1201 tcctaccgcc ggagccattg ttcgccggac cgagccacga ccccggcccg tcggtcgtcc
     1261 cggggtgtgg agcgtcccat gatccagcgc gagcaggttc ccgagagcac cggtaccgtg
     1321 gcgcgatgaa cgagctggcg cgcggggtcc agcgcaccta tctgatcctg accctgctca
     1381 cgaccctcgc ggcgtcgttc atctggggca tcaacaccct gttcctcctc gacgcgggcc
     1441 tgagcaacac cgaggccttc accgcgaacg cgttcttcac tctcggccag gtcatcttcg
     1501 aggtgccaac cggtgtcgtg gcggacaccc gtgggcggcg attctcgttc ctcctgggcg
     1561 ccttcacgct cctcgtctcc accctcctct atctcctgat gtggaacatc gaggccgcgg
     1621 ccgaggagaa cggccggtcc gtcgccgaga ccctcgacat catggaacgc acgaaagcga
     1681 ttgaccgcgg ggatcacccg gacgagtacg agtccgccta ggtcgaggcc cggggatctc
     1741 gcgggcgggg agcctataat ccgccggcgt tccggcgtag ctcagtggta gagcagacgg
     1801 ctgttaaccg tcgggtcgta ggttcgaatc ctaccgccgg agccatttct cgccggaccg
     1861 agccctgtcc ccggcccgtc ggtcaatccg gggtgcggag cgttcgcatg atccagcgca
     1921 ggcatggtct cgagagcacg ggtaccgtga ggccgtgaac gaactggccc gcggggtcca
     1981 gcgcacgtac ctgatcctca ccctgctgac gacactcgcc acgtcgttca tctggggcat
     2041 caacaccctg ttcctgctcg acgccggcct gagcaacacc gaagccttca ccgcaaacgc
     2101 gttcttcacc ctcggccagg tcatcttcga ggtgccgacc ggggtcgtcg cggacgtccg
     2161 aggccggcgt ttctcattcc tcctgggcgc ggggaccctc ctcctctcca ctctcctgta
     2221 cctggtcatg tggaacatcg aggccccgtt ctggggctgg gccgtcgcgt ccgccctgct
     2281 cgggctggga ttcaccttct tctccggcgc gaccgaagcg tggctggtgg acgcactcca
     2341 cgcatcgagc ttcaccggga ccctggagtc ggtcttcgcc agggcccaga tcgtcggcgg
     2401 cgtggccatg ctcagcggat cggtcctcgg cggcttcgtg gcccagctca cgaacctcgg
     2461 ggtgccctac atcctgcgag cactgatgct gggcgtgacc ctggtggtcg cgtggtggtt
     2521 catgaaggac atcgggttca cgccccagcg cggtgccagc ccgatcgggg cagtcaagaa
     2581 cgtcgtgagc ggcgccgtcg atggcggcct ccgaaacccc ccggttcggt ggctgatgct
     2641 ggccgccccg ttcagcgccg gcgtgggcat ctacgccttc tatgccatgc agccctacct
     2701 cctccagctg tacggcgatc ccaccgccta tggcgtggcc ggcatagccg ccgcgatggt
     2761 ggccggtgcg cagatcctgg gcggaaccct cgtcccgtgg ttccgtcggt tcttccgcct
     2821 gcggaccgac gctctgatcc tgggtgccgt cgtgtcctcg ctcctcctcg tcctcatcgg
     2881 cctggtcacc tcgttcgtgt tggcgctcgt cctgctcgca atctggtgca tttcgctggc
     2941 gctcgtctcg cctctccgcc aggcgttcat caacggcgtc atcccctccg agcagcgcgc
     3001 cacggtcctg tccttcgatt ccctgatggg ttcggccggc ggagtcgtcg cccagccgat
     3061 cctcggtcgg tcagccgacg tcttcggcta cgccacctcg tacgtgatcg ccgggatcat
     3121 cagcgccgcg gtcatcccgt tctcgttcct cgctcggcgg gagcacgcac cttcggatcc
     3181 gatcaaggcg gaggagccag gctgacgcat cgcctagccg atcgtccgga actccaattc
     3241 ggtcttcagc gacatcgcct cgccgggcgg cagcagggcg agcgctccgg tttcgccctg
     3301 caacagacga cggagtcctt gcggcgcgtg cgtctgtggc tccacggcaa tcgcatcgac
     3361 gcctggcgga ctggccgcga cgatgaagag ggagggggcc gtcacccgca tcgtcgcgcc
     3421 gatcccgagc tgcggccacg cgagctcgac cggcggcgaa gcgaggtcgg tccatgtcgc
     3481 gtcgaggccg ggccgcatgg tctggagccg gcgcagatcc agcgacccct cgaccggccg
     3541 cgggtcgggc ggcgtgtcgg cgttggggct gaagacggca gcggcgcgga ttgcaactcg
     3601 gaccggtcgg cggaaccagg gatgaatgcc cagcccggcg ggcatcgggt cgtccgagag
     3661 attacggagc ccgatgtcca cgcggaggtg gtgctccgcg actgcgatct ggagcgtgga
     3721 ttcgtaccgc cagggccaac catcgccacc ggcttccact cggaaggcgc cgtcgggcag
     3781 gatctcccac tgtcggtcga ggacctcgcc gtggatggcc gtgccgtcgg cgaagttgct
     3841 gccgagctga atgggccgcc cgccgaccgt cacaggatcg gtcccgatcc ggttgcacca
     3901 cggagccatc acgaacgatc cccagaagaa cggctcgcgg tcgtaggctg cgaggtccgg
     3961 cggcgtccgg atcagatcat ggccgaggac ctggagccga tggagacggg ccccgcggtt
     4021 cggcaggacc acggcccgaa cggtctcgcc gacgaagacg gcttcggatc cggccaaggc
     4081 ggacctccta gcccgccatg gtcatcgccc gctcggggcg aagcaggaac gggggtaggt
     4141 aggcgggcat gttcgagaac gggagcacgt cgggatggag ctcgatccca agcgcatcgc
     4201 gcatgaactg tcggcgtgct ccgatccggc tccacgcgtc cggataacgc gtggcaaagg
     4261 tctcacgcag agccgcgtcg gccagggcga tgccgtcctc gatgttcgtc gtgaagtagt
     4321 cggtcccggt ggcggggatg atgtcgacct ggagcgccat cccggaccgc agctcgaccg
     4381 atgagctccg cgcgaccggg gagttcaccc actcgtcgag gtgaatctgg tgcccggggt
     4441 tgagcgtgat cccgaagaag gggtccccca ggcgccggtc gattatgtcc tgcagggcac
     4501 cgcctgtctg accgatgtgg agcgcctcgt accactccgc cacggcggcg aagtagggtc
     4561 cgaccagccg ctcgacgtag tcgcgaatgc ccgccggcag ccactgctcg ccttccacga
     4621 tgaacccggc gcggcagttc agcgcacccc agatgccata tgcgacggtg aaccggtcgc
     4681 cgtgctccac ggtgcgatcg cccgggctaa gcagaccgag cgtggcgcgc ggcccgctgg
     4741 tcagcatgag gtggcaggag aggggcgccc cgttccactc caggaggctc accgcctggt
     4801 gctcggtcat ccccggccgc aggccgaaca acagccggcg gaccgcgttc gaggtgtgac
     4861 aggccgcgtg ctcgaaggcc gcgagctgct ccacctcgtt gatgacccgg aggccgttcc
     4921 cgggatggat caatagatcc gtggcgttga cgaccgatcc ggtcgggccg gtagcgcgcc
     4981 gcaactcgtc gaccagatac gacggcgcgt cgctggcggc agggtcggca tacgtcttcc
     5041 agccgatgac gccgacccgc gagccgggac cgatgccctc cgcggccagc agctcggccc
     5101 atggtcgcga ccggtcgcgg ggctgggagg gcaggctcag gtcctggatg agctggcgcc
     5161 gcatggtgag tggggcggct ccggccaggc cgaagcactc gttgccgacc atgatcgcgg
     5221 gcgggtcgtc cggtccgatg accaggaccg cttcctcgaa ccgcggatcg aacccggtga
     5281 ggtaggcgag gttcgcgctg tgctcccggt ccgcgtacac cacgagtcgg tcgaaccccg
     5341 cctcgtccgc tcgcgctcgc aggcgtccgc cccgttgctc gtagagctcg gtcgggatgc
     5401 ccggcgtgct cggcgacgca ccaactgcgg ggaggctgag ctcggccatg cgcgcacgcc
     5461 ggtggtggct cacggctggg tt
//
LOCUS       DASYGU010000135         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_279441, whole genome shotgun sequence.
ACCESSION   DASYGU010000135 DASYGU010000000
VERSION     DASYGU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279441"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGO32_03565"
     CDS             <1..411
                     /locus_tag="VGO32_03565"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HEV7199859.1"
                     /translation="RDSAKGRDLAANPRAAAVFWWEGTNRQVRVSGAVAPVDDAESDA
                     YWASRPVGHQLAARASAQGEPVDDRPSLEAQVEAMRSRFGPDGPVPRPPSWGGYRLTA
                     EVVELWESREDRLHDRIEYQRRPDGGWDRRRLQP"
     gene            complement(408..833)
                     /locus_tag="VGO32_03570"
     CDS             complement(408..833)
                     /locus_tag="VGO32_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7199860.1"
                     /translation="MDVEAALAATPLLSSLDRKTIKRLAEQGKHRTYAAGETIVKEGA
                     PASALYIILSGKVHVEREGGGDGRPPMLGAGDFFGELALIEEHPRSATVVADETTDCI
                     LFVAWEFTALLKEYPEMAIPLMNALIARLHRREHHLASG"
     gene            complement(886..1062)
                     /locus_tag="VGO32_03575"
     CDS             complement(886..1062)
                     /locus_tag="VGO32_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199861.1"
                     /translation="MPFLPADPIIVLMALAGLILGTMAYIRILRRRHRDRRLDKEADA
                     EQLRGLVRVTLIGH"
     gene            complement(1145..1585)
                     /locus_tag="VGO32_03580"
     CDS             complement(1145..1585)
                     /locus_tag="VGO32_03580"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEV7199862.1"
                     /translation="MVILLLIHVLAAITAVGANLTYAIWFRAAGTDQDRLVFVIRTVR
                     AIDSRLANPAYIVLLITGVLMVLSGAYSFTTGWILAAIGLYIAVAVIGIAAFGPAIRR
                     QLVEAERDVTSSAYQAAARRSTTLGLITTALVLLIVVLMVTKPF"
     gene            complement(1588..2145)
                     /locus_tag="VGO32_03585"
     CDS             complement(1588..2145)
                     /locus_tag="VGO32_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7199863.1"
                     /translation="MTEPTSFTVESSPDPIDAPKAYQDLLVGLVGTDDPAEVQAATPE
                     LIRALIAEAGPDLRIRPAASEWSVLECVAHITDAELVASGRYRWILAHDEPELVGYDQ
                     DLWVNHLHQPTEEPEVLLALFEPLRQANLGLRARTPVEARSRIGMHRERGPESYELTF
                     TLIAGHDRLHVAQAERALELVRSAR"
     gene            complement(2174..3067)
                     /locus_tag="VGO32_03590"
     CDS             complement(2174..3067)
                     /locus_tag="VGO32_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7199864.1"
                     /translation="MDVGVVLPQGWTGEYDGWDPRRAWERTVAVAQQAERLGFESIWV
                     FDHFTTEPEPTDELTFESFTTLSALAVLTTRVRIGQVVICTAFRNPALTAKMIGTMDL
                     ISGGRMELGIGAGWKEDEWRAYGYGFPDTKTRLAMLRDHLEVITRMLAPGHATYAGEH
                     ASVTDAINEPRGLQEPRVPIMVGGNGPNVTWRLAARFADELNLDWLSPDEVRGAREVI
                     ASRCEEIGRDPAMLRISVNISRFDVATPGPARVELLAGHRDAGVDRIMALLQASATED
                     EALEQFAEDARAAGCTLTPVG"
     gene            3324..>3715
                     /locus_tag="VGO32_03595"
     CDS             3324..>3715
                     /locus_tag="VGO32_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199865.1"
                     /translation="MAVELLKAPGGALMGNVPSPSRRGFARAITIALTAAFSLSLILA
                     GTASAATPHRWHDDHLLYLAPDYAQTQPCPRATDAWLGMNTSGKDSPLDSNIRGWKVV
                     IPAVDPALGNCAVSGWTDASLVMNIPIA"
BASE COUNT          586 a         1236 c         1312 g          581 t
ORIGIN      
        1 cgggacagcg cgaagggccg ggacctggcc gcgaacccgc gggcggcggc cgtcttctgg
       61 tgggaaggca cgaaccgcca ggtacgggtc agcggcgcgg tcgccccggt ggacgacgcc
      121 gaatccgatg cctactgggc ctcgcgcccg gtcggccacc agctcgcagc ccgggcgtca
      181 gcccagggcg agcccgtcga cgatcgccca tcgctcgagg cacaggtaga ggcgatgcgg
      241 agccgattcg ggccggacgg gcccgtgccg cggccgccct cctggggcgg ctatcggctg
      301 accgccgagg tcgtcgagct atgggagagc cgcgaggacc ggctccatga tcggatcgag
      361 taccagcgcc gccccgacgg cggctgggat cggaggcgtc tgcagcccta gcccgaggcc
      421 aggtggtgct cccgccggtg gagacgggcg atcagggcgt tcatgagcgg gatcgccatc
      481 tcgggatatt ccttcagcaa cgcggtgaac tcccacgcca cgaacaggat gcaatcggtg
      541 gtctcgtccg ccacgaccgt cgcgctgcgg gggtgctcct cgatgagcgc cagctcgccg
      601 aagaagtcgc ccgcgccgag catgggaggt cgcccgtcac cgccgccttc gcgctcgacg
      661 tggaccttcc cgctcaggat gatgtacagg gcggaggccg gcgcgccttc tttgacgatg
      721 gtctcgccag cggcgtaggt tcggtgtttg ccctgctccg cgaggcgttt gatcgtcttg
      781 cggtcgaggc tcgaaagaag cggggttgcg gcgagagcgg cttcaacgtc catgaccgca
      841 tggtagcgcc ggatcggcgt cgcgggaagg tctcttgccg tcggctcagt ggccgatgag
      901 ggtcacccga acgaggccgc ggagctgctc ggcatcggcc tccttgtcca accgccggtc
      961 acgatgacgg cgtcggagga tccggatgta ggccatggtg cccaggatga gcccggcgag
     1021 ggccatcaga acgatgatcg ggtcagccgg caggaacggc atgcccggag cgtgggcgcc
     1081 gccgcggccc gcgggtatgg gaggaaggta ccgaggcagc ccgtcacttt ggcggacgag
     1141 ccggtcagaa cggcttggtc accatcagga cgacgatgag gaggacgagc gcggtcgtga
     1201 tcaggccaag ggtcgtggac cggcgggccg ccgcctggta ggccgatgac gtgacgtccc
     1261 gttcggcttc gacgagctgg cgtcgaatgg ccggcccgaa cgcggcaatg ccaatcaccg
     1321 cgacggcgat gtacagcccg atggcggcca ggatccagcc ggtcgtgaag ctgtacgccc
     1381 cgctcaggac catgagcacg ccggtgatga gcagcacgat gtaggccggg ttggcgaggc
     1441 ggctgtcaat cgcccgcacc gtccgaatga cgaagaccag ccgatcctgg tcggtgccgg
     1501 cggcgcggaa ccagatcgca taggtcaagt tcgccccgac cgcggtgatg gccgccagga
     1561 cgtgaattag cagcaggatg accacggtca gcgggccgag cggaccagct ccagggcgcg
     1621 ctcagcctgg gccacgtgca ggcgatcgtg gcccgcgatg agggtgaagg tcagctcgta
     1681 gctctccggt cctcgctcgc ggtgcattcc gatccgggag cgggcctcga cgggcgtccg
     1741 ggcccgcagc ccgaggttcg cctggcgcag gggctcgaac agggcgagca gcacctcggg
     1801 ctcctcggtc ggctggtgga ggtggttcac ccacagatcc tggtcgtagc ccaccagctc
     1861 cggttcgtcg tgagccagga tccatcggta ccgaccgctg gccaccagct ccgcgtcggt
     1921 gatgtgggca acgcattcca ggacggacca ctcgctcgcc gccggccgga ttcgcagatc
     1981 gggtcccgcc tcggcgatca gcgcacggat cagctccggt gtcgcggcct gcacctcggc
     2041 tgggtcatcg gtccccacca ggccgacgag caggtcctgg tacgccttcg gggcgtcgat
     2101 cggatccggg ctggactcga cggtgaacga ggtcggctcg gtcatcgggc tgaggatacc
     2161 cggcaggcgg ccgtcatccg accggcgtca gcgtgcagcc tgccgcccgc gcatcctccg
     2221 cgaactgctc cagggcctca tcctcggtcg cgctggcctg gagcagggcc atgatccgat
     2281 cgacgccggc atcgcgatga ccggccagca gctcgacccg agccgggccc ggcgtggcga
     2341 cgtcgaaccg gctgatgttc accgagatcc gcagcatcgc cgggtcgcgt ccgatctcct
     2401 cgcagcgcga cgcgataacc tcccgcgcgc cgcggacctc gtccgggctg agccagtcca
     2461 ggttgagctc atcggcaaag cgcgccgcca ggcgccaggt cacgttcggc ccgttgccgc
     2521 cgaccatgat cggcacccgc ggctcctgga ggccccgggg ctcgttgatc gcgtcggtca
     2581 ccgaggcatg ctcgccggcg taggtcgcgt gtcccggcgc gagcatccgg gtgatgacct
     2641 cgaggtggtc gcggagcatc gcgaggcggg tcttcgtgtc ggggaacccg taaccgtagg
     2701 cccgccactc gtcctccttc cagccggctc cgattcccag ctccatccgg ccgccgctga
     2761 taaggtccat ggtcccgatc atcttggccg tgagcgcggg gttgcggaat gccgtgcata
     2821 tgaccacctg accgatgcgg acgcgcgtgg tgagcacggc cagggccgac agggtagtga
     2881 acgactcgaa ggtcagctca tcggtcggct cgggctcggt cgtgaagtgg tcgaagaccc
     2941 agatcgactc gaaacccagg cgttccgcct gttgagccac ggcgaccgtc cgctcccagg
     3001 cccgccgcgg gtcccatccg tcgtactcgc cggtccagcc ctgcggcagg accaccccaa
     3061 cgtccattca ccgaacgata cccgtcctgc ctggagccgg ggttggcgca tggctgcatt
     3121 gcaatttcgc gacaatttct ggctgtgaaa tgaggaccgc cgccgtcttc cgcgatcgga
     3181 tacggcgcac aggaccaccc gagtggcgac aggaatcaac ggaagtgccg ccgtcacgat
     3241 ttccggcagc ggcttcgctc tatgattttg tggggtcgcg catggtgcgg atcattgcgg
     3301 tggggtcgcg catagcccgg accatggcgg ttgagctgtt gaaggcccca ggaggtgccc
     3361 tgatgggcaa tgtgcccagc ccgagccgtc gcggattcgc gcgagcgatc acgatcgcgc
     3421 tgacggccgc gttttcgttg agcctcattc ttgcgggaac ggcatccgcc gcgacgccgc
     3481 acaggtggca cgatgatcac ctgttgtacc tcgcgcctga ctacgcgcag acgcagcctt
     3541 gcccaagagc caccgacgcg tggctgggca tgaacacgtc agggaaggac tcgccccttg
     3601 attcgaacat caggggctgg aaggttgtta tcccggcggt cgacccggcg ctgggaaact
     3661 gtgccgtttc cggctggacg gacgcatcgt tggtgatgaa catcccgatc gcgaa
//
LOCUS       DASYGU010000136         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1514187, whole genome shotgun sequence.
ACCESSION   DASYGU010000136 DASYGU010000000
VERSION     DASYGU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514187"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VGO32_03600"
     CDS             complement(<1..754)
                     /locus_tag="VGO32_03600"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV7199866.1"
                     /translation="MKAPLLPDETEMYRLMAARDPEAEGAFWVAVRTTGVFCRPTCAS
                     RTPLPHNVEFHATPWDALVAGFRPCKRCRPMHPRGAPRPSRDAVPEPELLITRIGTPI
                     GTMVAAAVEDGIAVLDFADRRATRTALRDASRRLKATPTIGRNRNLDTLETQLHDYFA
                     GTRTTFDLPLARRGTAFEESVWSWLVTIPAGETRTYAQGAAIVGRPKAVRAIGRANGR
                     NPVAIVVPCHRVIGSDGDLTGYGGGLWRKRALL"
     gene            complement(793..1296)
                     /locus_tag="VGO32_03605"
     CDS             complement(793..1296)
                     /locus_tag="VGO32_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEV7199867.1"
                     /translation="MTAERQFDPIRSFQTLNRHRVQYIVIGGFAGKLLGSTILTVDID
                     VCYSRDPKNLERLAAALRELHAELRGAPGGLPFRLDERALRKGDNFTFVTDAGDVDIL
                     GMPPGSGGYEGLRRNATELDLGEVTVWVAGIEDLIAMKRASGRLRDLAHLEVLSALRE
                     EIEAQAD"
     gene            complement(1293..1616)
                     /locus_tag="VGO32_03610"
     CDS             complement(1293..1616)
                     /locus_tag="VGO32_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7199868.1"
                     /translation="MQAARMLRHARRSAGMTQRQLAIAAGVPQSTVGRIETNRLNPTV
                     DNFRHLLRATGQDLDLVPLAGADEDRTLIRDRLRMTPAGRARLAVQEARFAARLGSRP
                     RARRS"
     gene            complement(1664..2113)
                     /locus_tag="VGO32_03615"
     CDS             complement(1664..2113)
                     /locus_tag="VGO32_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEV7199869.1"
                     /translation="MTARRLWRLGVGLFVAGVVVMVAIQFLPYGHAHSNPPVLAEPAW
                     DSPETRAYAVIACFDCHSNETVWPWYSNIAPVSWLVQRDVDHGRTVLNFSEWGRGEQE
                     AEDAAEKYAEGAMPLPVYLLMHPDANFPPVDREAFLAGLRATFGGGD"
     gene            2209..>2546
                     /locus_tag="VGO32_03620"
     CDS             2209..>2546
                     /locus_tag="VGO32_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7199870.1"
                     /translation="MSRSMKVGVQLPEVEWVPRWADIRDMALAAEEIGLDSIWVGDHL
                     LYRYLDDAGNETEPARGPWEAWTSLAAVAAITERIQLGPLVAATSFHNPAMLAKKAAT
                     LDEISGGRLIL"
BASE COUNT          385 a          844 c          917 g          400 t
ORIGIN      
        1 cgagcagggc gcgcttgcgc cacaggccgc cgccgtagcc ggtcaaatcg ccgtccgagc
       61 cgatgacccg gtggcaggga acgacgatgg ccaccgggtt gcggccgttg gctcgaccga
      121 tggcccgcac cgcctttggc cgtccgacga ttgccgcgcc ctgggcgtag gtgcgcgtct
      181 cgccggcggg gatggtcacc agccaggacc agaccgattc ctcgaacgcc gtcccgcggc
      241 gggcgagcgg caggtcaaat gtggtgcggg tcccggcgaa gtaatcgtgg agctgggtct
      301 cgagcgtgtc caggttccgg ttgcgaccga tggtgggcgt ggccttgagc cggcgggacg
      361 cgtcgcggag tgcggtccgc gtcgcccgcc gatcggcgaa gtccaggacc gcgatcccgt
      421 cctcgaccgc ggccgcgacc atggtcccga tcggcgtccc aatccgggtg atgagcagct
      481 cgggctcggg taccgcgtcg cgggacggtc gcggtgcgcc gcgtgggtgc atcggccggc
      541 accgcttgca gggtcggaac ccggcgacca gcgcatccca cggcgtggcg tggaactcga
      601 cgttgtgggg gagcggcgtc cgcgacgcgc acgtgggccg gcagaacacc ccggtcgtgc
      661 ggaccgcgac ccagaacgcg ccctcggcct cgggatcgcg ggcggccatc aatcggtaca
      721 tctcggtctc atcggggagc agcggcgcct tcatgtcacc tgaaacgtac ctcccggcgg
      781 atccgtgaga tctcagtccg cctgggcttc gatctcctcc cggagggctg acaggacctc
      841 gaggtgggcg aggtcccgca agcgacccga ggcgcgtttc atggcgatga ggtcttcgat
      901 gccggcaacc cagaccgtca cctcgccgag gtcgagctcc gtcgcgttgc gccgcagacc
      961 ttcgtagccg cccgatccgg gcggcatgcc gaggatgtcg acatcgcctg catcggtcac
     1021 gaacgtgaag ttgtccccct tgcgcagggc gcgctcgtcg agtcggaagg ggaggccccc
     1081 gggggcgccc cggagctcgg catggagctc gcgtagcgcc gcggcgagcc gctcgaggtt
     1141 ttttggatcc cgcgaatagc agacgtcaat gtcgacggtc aggatcgtgg accccaggag
     1201 cttgccggca aagccgccaa tcacgatgta ctggacgcga tgccggttca gggtctggaa
     1261 ggagcgaatc gggtcgaact ggcgttccgc ggtcatgatc gccttgcccg cggacgtgat
     1321 ccgaggcggg cggcgaaacg ggcctcctgg acggccagcc gtgcgcgacc ggccggtgtc
     1381 atgcgcaggc ggtcccggat gagcgtccgg tcctcgtctg cgccggcgag tggcaccagg
     1441 tcgaggtctt gaccggttgc tcgcaacagg tggcggaagt tgtccacggt ggggttgagc
     1501 cgattcgtct cgatccgccc gaccgtcgac tgcgggacac cggcggcgat cgccagctgg
     1561 cgctgggtca taccggcgct ccttcgagcg tgacggagca ttcgggcggc ctgcattcgg
     1621 ctgcatggta ccgcgttgat agcatttacg ctatcagggt ggactagtcg ccgcctccaa
     1681 acgtcgcgcg gagcccggca aggaacgcct cgcggtccac gggcgggaag ttcgcatcgg
     1741 ggtgcatcag caggtagacg gggagtggca tcgcgccctc ggcgtacttc tccgccgcgt
     1801 cctcggcttc ctgctcgccg cggccccact cggagaagtt cagcaccgtc cgtccatggt
     1861 cgacgtcgcg ttgcaccaac cacgagacag gggcaatgtt cgaataccag ggccacacgg
     1921 tctcgttgct gtggcagtcg aagcacgcga tcacggcgta tgcccgagtc tcgggcgagt
     1981 cccacgccgg ctcggcgagg accggcgggt tcgaatgcgc gtgcccgtac ggtaggaact
     2041 ggatcgccac catcacgacg accccggcga cgaacaggcc aaccccgagc cgccacagac
     2101 gtcgtgctgt cacgaaccca tagtgctgcc ccaggccgtg tcctgccaag tgccgtgtgg
     2161 tccccgctcg cgggtgatgc ggggtgggcg atgcgcgaca attcccaggt gagcaggtcc
     2221 atgaaggttg gggtccagct tcccgaggtg gagtgggtgc cgcgctgggc ggacatccgc
     2281 gatatggcgc tcgcggccga ggagatcggt ctggactcca tctgggttgg cgatcacctc
     2341 ctgtatcggt atctcgacga cgcgggcaac gagaccgagc cggctcgcgg gccgtgggag
     2401 gcatggacca gcctcgccgc ggtggccgcg atcacggagc ggatccagct cggcccgctg
     2461 gtggccgcca cctcgttcca caacccggcg atgctggcca agaaggccgc caccctggac
     2521 gagatcagcg gcggccggct gatcct
//
LOCUS       DASYGU010000137         6898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1720330, whole genome shotgun sequence.
ACCESSION   DASYGU010000137 DASYGU010000000
VERSION     DASYGU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720330"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGO32_03625"
     CDS             complement(<1..206)
                     /locus_tag="VGO32_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEV7199871.1"
                     /translation="MPKQLQFSEEARRSLKRGIDKMSDAVKVTLGPKGRYAVLDKKFG
                     APTITNDGVTIARDIELEDPYENMG"
     gene            complement(258..572)
                     /gene="groES"
                     /locus_tag="VGO32_03630"
     CDS             complement(258..572)
                     /gene="groES"
                     /locus_tag="VGO32_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV7199872.1"
                     /translation="MAKTATATKTQLKPLGDRLVVKPTPREEMTKSGIVLPDTAKERP
                     QEGTVLAVGPGRTLDDGTREPMELSVGQKILFQKYAGTEFKLDEDELLILSQKDILAV
                     IE"
     gene            complement(1042..1953)
                     /locus_tag="VGO32_03635"
     CDS             complement(1042..1953)
                     /locus_tag="VGO32_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV7199873.1"
                     /translation="MGRGGWGATHRNAHSAVPADRGRACGRSGLRSHRVLSRLVVPER
                     LPGIVDSHAHLQHEVFAHDLDAVLDRARAGGLLRILVPGWDRASSEAAVALGAQHPDL
                     LDVAVGIHPHYVAAATETDWTAVETLAADTGVKAVGEIGLDFYRNLSPPDIQRDALAR
                     QLDLAARVHKPTIVHDREAHEEVTAALVAHASHGAGVPGILHAFSGDAAMAHALVTAG
                     YLISFALTVSFPKNTGPRDAAATLPIGNLLVETDSPYLGVAKDQRNEPTTVLRTAAVL
                     ARLRNEDPELIGAAARSSYRRLGIIDR"
     gene            complement(1845..3437)
                     /locus_tag="VGO32_03640"
     CDS             complement(1845..3437)
                     /locus_tag="VGO32_03640"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV7199874.1"
                     /translation="MTETFYLTTAIFYPTPRPALHSLFEAIGADALVRYHRLTGKDVR
                     YLTGMDEHSANVERLAAERGTEPRPLVDEWAASWQAGFDRFDVSYDRFIRTTDADHRE
                     ASIEMVRRAQAAGDIYKGTYAGWYCTGDNEFKTEQQIVDGRCPEHPTLELQWLEEENW
                     FFALSKYQAALEELYRTNPGFCEPEHFRNEVLGWLADGLQDFSVSRSGTGWGIPFPGD
                     EEHRIYVWFDALTNYVTGAGFPGDTAAFEKWWPADVHVIGKNITRFHCLYWPAMLMSA
                     GLPLPKKVFAHGFMLMRGEKMSKTRGNVLDPDQALATFGVDGVRYLVLREIPFDRDGD
                     VSADGMIRSYNADLANDLGNLVNRTVSMAGRYLEGVLPDLGGLTQTDADAELQRVAAS
                     AVTGYRAAIEQLHFDEALTALSSLTRAANGYAESQAPWALHKAGEVERVGAVLATMGE
                     ACRILGHLLAPFCPTASANLLGQIGTPPAYDGRGSGGPGIDVLLTWGAEAGERRTGTP
                     TPLFPRIEDEPVDGAASGATAS"
     gene            complement(3434..4219)
                     /locus_tag="VGO32_03645"
     CDS             complement(3434..4219)
                     /locus_tag="VGO32_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7199875.1"
                     /translation="MSERYPYNARLSRWDVVSDGLVICAVALDGEPFPFLPGQYNTLG
                     LDDPDGKTILRPMSISSPSTDLSEYEFFIRHVREGEFTPLLWEIKLGDPLFMKGAKGK
                     FLLQDDGKQCLFVASGTGLAPFMSMIETLLATGESREIHLLHGVSHAHDLAWREWLTE
                     IEADQRLPLQYAGTVSRPQANPDWTGLTGRVETVVADELDRRGLTADNTTIYLCGNPE
                     MITAVDEIATERGFPPEQVRKELYWPKARPHGPAVVVDVEPIP"
     gene            complement(4330..5037)
                     /locus_tag="VGO32_03650"
     CDS             complement(4330..5037)
                     /locus_tag="VGO32_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7199876.1"
                     /translation="MSTTPIVLVPGFWLGAWAWDEVAAILRADGHEVTALTLPGMESA
                     DADRSSITLSDHVDAIVEAVRAAGSPVVLAVHSATGFSGYVASDRVPELIAAMVYVDT
                     APGIPPLDPDFTDAEKPMDWASVSEEENLDGLSEEHLAMFRQRAVPVPGGVLRGTAAL
                     TNDKRRDIPSTLICTGFTSEQYKESVSEGYSWLAGIPELRNPTWIDLPTSHWPMWSRP
                     QELAAIIGDVAKGSSPS"
     gene            5100..6131
                     /locus_tag="VGO32_03655"
     CDS             5100..6131
                     /locus_tag="VGO32_03655"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7199877.1"
                     /translation="MAGKAAGPRLDLTREQILAFRRAVAALDERLPAGPDSLRRAAWA
                     GLQDSMPRAALLSIHARVEWTEPTTWEDPSLVQVWGPRFSTYVIPRPDLAVFTLGRYP
                     DDTRSRRVAEEMADKMAGVLGDQRMPDREVAGALGVGNGIRYAGPTGTILIRWEGARA
                     PTIWTVPRPDVDPGDARRELARRFLHVFGPATPDSFSRWAGIGTAAGQATFDTLGSEL
                     TPVTTPIGESWILTVDEPAIRAPAAPAAAARLLPSGDTYFLLQGVDRELLVPEANRRA
                     LLWTSRVWPGAVLVAGEVVGTWRRANANLSIKSWRRLTAPERAAVEAEAASLPLPGLR
                     GPIRVRWDT"
     gene            6164..6793
                     /locus_tag="VGO32_03660"
     CDS             6164..6793
                     /locus_tag="VGO32_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7199878.1"
                     /translation="MSEEGRREFLAADGVDDWVVLHGGAAAVFRAESLGEAARLAVAI
                     ADVARFEGSGALLTISGTGLTVRLTRDLWVLEERHVELARAVSAVARLHGAVADRSAV
                     QEVQLAIAAKPDAMDVDFWRAVLGYAPMSPDNAVDPLGHGSTVWMQGLDEAKPLRHAM
                     HVDVSVAREHAETRLAAALAAGGRIVDDSSAPGAWILADRAGNKVCIAA"
BASE COUNT         1028 a         2276 c         2453 g         1141 t
ORIGIN      
        1 cccatgttct cgtacgggtc ctcgagctcg atgtcgcggg cgatggtgac gccgtcattg
       61 gtgatggtcg gcgcgccgaa cttcttgtcg aggacggcgt agcggccctt gggaccaagc
      121 gtgaccttga ccgcgtcgga catcttgtcg atgccgcgct tcagggagcg ccgggcttcc
      181 tcgctgaact ggagttgctt gggcatggtg gtgggaattc ctccgaagct gaactgggtg
      241 gagcgaactg aatcgggcta ttcgatgacg gcgaggatgt ccttctggga caggatcagc
      301 agctcgtcct cgtccagctt gaattcggtg cccgcgtact tctggaacag gatcttctgg
      361 cccacgctga gctccatcgg ctcgcgggtg ccgtcgtcga gcgtgcggcc cgggccgaca
      421 gccaggaccg tgccctcctg ggggcgctcc ttggcggtgt cgggcaggac gatcccgctc
      481 ttggtcatct cctcgcgggg agtgggcttg acgaccagcc gatccccgag gggcttcagc
      541 tgggtcttcg tggcagtagc cgttttggcc atggaagcgg tgaccctcca gatgtctgcg
      601 caaccgatgc cgggggcggc atcgactggg attgcgtttg atgcactgcc gctgggattg
      661 ccggatggac cgatacgatc cgtttccccg ttgggggttc tggcactcga ccaacgagag
      721 tgctaaacca aggtatcagg tgcgctcgtc cactgtcaag gcgaccgccc ccacgaccgt
      781 ccttcgttag cggcccattg acgtacctcg ccgggaccac gcgtcccggg agtcgcgtgc
      841 ccgtcctacg gacgtatccg ccaagaatgt tggcggtcag gctcagattg tccactccac
      901 ggacctatcg gccacgaatg ttggccggca gggtccgatt gtccactcca cggacgtatt
      961 ggccaaagaa ggggatcccg gagctcaatt cggactcaaa cctggccgat gtgtccgtcc
     1021 cgtggacatc gcaggacggg tctaccgatc gatgatccca aggcggcgat acgagctgcg
     1081 ggcggccgcg ccgatcagct ccggatcctc gttccggagg cgggcgagga cggctgcggt
     1141 gcggaggacg gtggtgggct cgttgcgctg gtccttcgcc acgcccaggt acggggaatc
     1201 ggtctcgacc agcaggttcc cgatgggcag ggtggcggcc gcgtcgcgcg gaccggtgtt
     1261 cttggggaag ctgaccgtca gcgcgaacga gatgaggtag ccggcggtca cgagggcgtg
     1321 ggccatggcc gcgtccccgg agaaggcgtg gaggatgccg ggcacgccgg cgccgtggga
     1381 cgcgtgggcg acgagcgcgg cggtcacctc ctcatgggcc tcacggtcgt ggacgatcgt
     1441 cggcttgtgg acccgggccg cgaggtccag ctgccgcgcg agggcgtccc gctggatgtc
     1501 cggcggggag aggttgcggt agaagtccaa tccgatctcc cccaccgcct tcacgcccgt
     1561 atcggcggcc agcgtctcga ctgccgtcca gtccgtttcg gtggccgcgg ccacgtagtg
     1621 cgggtggatg cccacggcca cgtcgaggag gtccgggtgc tgggcgccga gcgcgaccgc
     1681 cgcctccgac gaggcccgat cccagcccgg aaccaagatc cggagcaggc cgcctgcccg
     1741 agcccggtcc aggaccgcgt caaggtcgtg ggcgaagacc tcgtgctgga gatgggcgtg
     1801 actgtcaacg atccccggca ggcgctcggg aacgacgaga cggctcagga cgcggtggct
     1861 cctgaggccg ctccgtccac aggctcgtcc tcgatccgcg ggaacagcgg agtgggcgtt
     1921 ccggtgcgtc gctccccagc ctccgcgccc caggtcagga ggacgtcgat gcccggtccg
     1981 ccgctccccc gcccgtcata agcgggtggg gtgccgatct ggcccagcag gttcgccgac
     2041 gcggtcgggc agaacggagc gaggagatgg cccaggatcc ggcacgcctc ccccatcgtg
     2101 gcaagcaccg cacccacgcg ctcgacctcg cccgccttgt ggagcgccca cggcgcctgc
     2161 gactcggcgt agccgttcgc ggcgcgggtc aggctggaca gtgccgtgag cgcctcgtcg
     2221 aagtgaagct gctcgatggc ggcacggtag cccgtcaccg cgctcgcggc cacgcgctgg
     2281 agctcggcat cggcatcggt ctgggtgagg ccgcccaggt ccgggagcac cccctccagg
     2341 tatcggcccg ccatcgagac cgtccggttg acgaggttcc cgaggtcgtt ggcgaggtcc
     2401 gcgttgtagc tgcggatcat gccgtccgcg ctgacgtcgc cgtcccggtc gaacgggatc
     2461 tcgcgcagga cgaggtaccg gacgccgtca accccgaatg tggccagcgc ttggtccgga
     2521 tccaggacgt tgccgcgggt cttggacatc ttctcgccgc gcatgagcat gaagccgtgg
     2581 gcgaagacct tcttgggcag cgggagaccg gcgctcatga gcatcgccgg ccagtagaga
     2641 cagtggaagc gggtgatgtt cttgccgatg acgtggacgt cggccggcca ccatttctcg
     2701 aacgcggccg tgtcgcccgg gaagccggcg ccggtgacgt agttcgtgag cgcatcgaac
     2761 cagacgtaga tccggtgttc ctcgtcgccc gggaacggga tgccccatcc ggtgccggat
     2821 cggctcacgg agaagtcctg gagcccgtcg gccagccacc caaggacctc gttgcggaag
     2881 tgctccggct cacagaagcc cgggttcgtc cggtacagct cctcgagcgc ggcctggtac
     2941 ttgctgagcg cgaagaacca gttctcctcc tccagccact ggagctcgag ggtcgggtgc
     3001 tccgggcagc ggccgtccac gatctgctgc tcggtcttga actcgttgtc gccggtgcag
     3061 taccagccgg cgtacgtgcc cttgtagatg tcgccggcgg cctgggcccg gcggaccatc
     3121 tcgatcgagg cctcacggtg gtcggcgtcg gtggtccgaa tgaatcggtc atagctgacg
     3181 tcgaaccgat cgaacccggc ctgccagctc gcggcccatt cgtcgacgag ggggcgaggc
     3241 tcggtcccgc gctcggccgc cagccgttcg acgttggccg agtgctcgtc catgccggtc
     3301 aggtagcgca cgtccttgcc cgtaagccgg tggtaacgga ccagcgcatc ggcaccgatg
     3361 gcctcgaaca gggagtggag cgccggcctc ggggtcgggt agaagatggc ggtggtcagg
     3421 tagaacgtct cggtcacggg atgggctcca cgtccacgac caccgccggg ccatgcgggc
     3481 gggccttggg ccagtacagc tccttgcgga cctgctccgg ggggaatccg cgctcggtgg
     3541 cgatctcgtc cacggccgtg atcatctccg gattgccgca gaggtagatc gtggtgttgt
     3601 ccgccgtcag cccgcggcga tcgagctcgt cagcgacgac cgtctcgacg cggccggtca
     3661 ggccggtcca gtccgggttg gcctgcgggc gggagaccgt gcccgcgtac tggagcggca
     3721 ggcgctgatc ggcctcgatc tcggtcagcc actcgcgcca ggcgaggtcg tgggcatggc
     3781 tcacgccgtg gagaaggtgg atctcgcgcg actccccagt ggccagcagg gtctcgatca
     3841 tgctcatgaa cggggcgaga ccggtgccgg aggcgacgaa caggcactgc ttgccgtcgt
     3901 cctggagcaa gaacttgccc ttggctccct tcatgaacag cgggtcgccg agcttgatct
     3961 cccacaggag tggcgtgaac tcaccctccc tcacgtgacg gatgaagaac tcgtattcgc
     4021 tcaggtcggt ggatggggag gagatggaca tcggccggag gatcgtcttc ccgtccggat
     4081 cgtcgaggcc gagggtgttg tactggcccg gcaagaacgg gaacggctcc ccgtccaggg
     4141 cgaccgcgca gatgacgagc ccgtcggata cgacgtccca tcgactcagc cgcgcgttgt
     4201 aggggtaccg ctcgctcacg gattctccgg atcatcgcgg cgcgaagatg ggccgccggg
     4261 ggcggatcga tggccgcggg cggccagtat atcggccgct tctgggccat ccggtccgtg
     4321 agaccaaggt caggaggggg atgagccctt tgcgacgtcg ccgatgatcg cggcgagctc
     4381 ctgcgggcgc gaccacatcg gccagtgact ggtgggcagg tcgatccagg ttggattgcg
     4441 cagctccggg atgccggcga gccaggagta gccctcgctc accgactcct tgtactgctc
     4501 ggacgtgaac ccggtgcaga tgagggtgct gggaatgtcg cggcgtttgt cgttcgtcag
     4561 ggcggcggtg ccgcgaagga cgccgccggg taccgggaca gcccgctggc gaaacattgc
     4621 caggtgctcc tcgctgagcc cgtccaggtt ctcctcctcc gacaccgacg cccaatccat
     4681 gggcttctcg gcgtcggtga agtcgggatc cagcggcggg atgcccggcg cggtgtcgac
     4741 atagaccatg gccgcaatca gctccggcac ccggtcgctg gccacgtagc ccgagaaccc
     4801 ggtcgcactg tgcacggcga gcacgaccgg cgagccggca gcgcggactg cctcgacgat
     4861 cgcatccacg tggtcagaga gcgtgatcga ggaccgatcg gcgtcggccg actccatccc
     4921 gggcagggtc aacgcggtga cctcgtggcc atccgcccgc aggatggcgg ccacctcgtc
     4981 ccatgcccac gcgccaagcc agaacccggg aaccaggaca atcggtgtgg tgctcatggg
     5041 tcccaaccat aaccgggtga gcctatccgg gtgtccgcgg acggcatact gacctcgcga
     5101 tggctggcaa ggcggcggga cctcggctgg atctcacgcg ggagcagatc ctggccttcc
     5161 gacgagccgt tgcggcgctc gacgagcggc tgccggcagg gccggattcc ctccggcgag
     5221 cggcatgggc gggcctccag gacagcatgc cgcgcgccgc cctcctgtcg atccacgccc
     5281 gtgtcgagtg gaccgagccg accacctggg aagacccgtc actcgtccag gtgtgggggc
     5341 ctcggttcag cacgtacgtc atcccgcgcc cagatcttgc ggtcttcacc ttgggtcgct
     5401 atccggatga cactcggagt cgccgtgtcg cggaggagat ggccgacaag atggccggcg
     5461 tcctgggcga ccagcgaatg cccgaccggg aggtcgctgg tgcgcttggc gtcggcaacg
     5521 ggatccgata tgccgggcct accgggacga tcctgatccg atgggaaggc gctcgggcgc
     5581 cgaccatctg gaccgtacct cgaccggacg tcgaccccgg cgacgcgcgc cgcgagctcg
     5641 cccgtcgctt cctccacgtc tttggcccgg cgacccccga ctcgttttcg cggtgggccg
     5701 ggatcgggac ggcggccggt caagccacgt tcgacaccct cggctccgag ctgacgccgg
     5761 tcacgacccc gatcggcgag agctggatcc tgaccgtcga cgagccggcg atccgcgcac
     5821 cagctgcgcc ggctgcggca gcgcggcttc tgcccagcgg cgacacctac ttcctgctcc
     5881 agggcgtcga tcgcgagctg ctcgtccccg aggccaatcg ccgagccctt ctgtggacct
     5941 cgcgcgtctg gcctggggcg gtgctcgtcg cgggtgaggt ggtcgggacc tggcgtcgag
     6001 caaatgccaa cctgtcgatc aagtcgtggc ggcgcctcac ggcccccgaa cgtgccgcgg
     6061 tggaggccga agcagcgtca ctaccgttac ccggtctccg agggcctatt cgcgtccgct
     6121 gggacacctg aacgtcgcct aacgtgtcgg aaggaggtcg agcatgagcg aagaaggccg
     6181 ccgggagttc cttgccgcgg atggtgtcga cgactgggtg gtgctccatg gcggggccgc
     6241 ggccgtgttc cgtgctgaat cgctcggcga agcggcgcgg ctggccgtgg cgatcgccga
     6301 tgtcgcccgc tttgagggct caggggcgct gctcacgatc tcgggcaccg gcctcaccgt
     6361 gcggctgacc cgcgacctgt gggtactcga ggagcgccat gtcgagttgg cgcgagcggt
     6421 ctcggcggta gccaggctcc acggcgcagt cgcggaccgc tcggccgtcc aagaggtgca
     6481 actggcgatc gcggcgaaac ccgacgcgat ggacgtcgat ttctggcgcg cggtgctcgg
     6541 ttatgccccg atgtcccccg acaatgcggt cgaccccctt gggcacggct ccacggtctg
     6601 gatgcagggg ctcgatgagg ccaagccgtt gcggcacgcg atgcacgtcg acgtgtcggt
     6661 cgcgcgcgag cacgccgaga ccaggctcgc ggcggcgctc gcggcgggcg gccgcatcgt
     6721 tgatgactcg agcgccccgg gcgcatggat cctcgccgat cgcgccggca acaaggtctg
     6781 catcgccgcc tagccggatg ggtctgtccc gccgccggcc ggtgacgcgg cctaggctcg
     6841 ttgtctggag gtctaccctt cgacgaccac ctgcagggag cgatgaccat gaagctga
//
LOCUS       DASYGU010000138         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_691694, whole genome shotgun sequence.
ACCESSION   DASYGU010000138 DASYGU010000000
VERSION     DASYGU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691694"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGO32_03665"
     CDS             complement(<1..375)
                     /locus_tag="VGO32_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7199879.1"
                     /translation="MRTTRSRVIGLVMAVLFLGGLFPAVAAAGGPTLNSEVIQSGLSY
                     PWDVAFTPDGKMLVTERGGNLKVFASGEPGAPLLRTNVVSYVRAQGEAGLMGIAVDRN
                     YATNHRIYVCASRSDYGQWLNQV"
     gene            complement(446..1450)
                     /locus_tag="VGO32_03670"
     CDS             complement(446..1450)
                     /locus_tag="VGO32_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199880.1"
                     /translation="MRRLPNRTALTAIVALMVLGVACGGVEGPGCLLIGYSEELQQDL
                     DTIAALDPALVAQAGTPENAAALAALDSLDATKATAQEALDAATDEEVGPVVRVSFQA
                     ALEATGTASSNLRDAIESGDAGAVASALDLVQLASDAIGAFQSVAEGLRTDCPGSSLT
                     PSEPAVSTPPSVAPTPAVTPAPTATPAPPPTATAEPTETEDETPTAAPTATPAPPATA
                     APTATAAPTATPAPTATASPTATPTATPSASASGSAEPSPSPSATPDDEGGGLLPWII
                     ILGLLGTGAAAVVLWYTNRNEPPPDDLSGMDDTAPIDGLPPAGPPPAGPPPTSPPPTG
                     "
     gene            1549..2286
                     /locus_tag="VGO32_03675"
     CDS             1549..2286
                     /locus_tag="VGO32_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV7199881.1"
                     /translation="MQNRRPRIVVTVQSPEHAANEEVAWRKNDRYFEAVTRHGGDVIV
                     LDEDSPAADRVNAFASMDGLLLSGGADLDPALYGEERHPTTVVEPGRDALEQAAFAAA
                     EERGVPVFGVCRGMQAVNVFRGGSLVQDIEGHTSPAYPAPEATSHDLEIEAGTRLAEV
                     LGEGPATVNTYHHQAVRPDQVGHGLRISGYSPHHDGDLVEALEDADPDRWLMAVQSHP
                     ERTEFTSPEFERLWASFLGAAGAFAAR"
     gene            complement(2301..>3091)
                     /locus_tag="VGO32_03680"
     CDS             complement(2301..>3091)
                     /locus_tag="VGO32_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEV7199882.1"
                     /translation="TGTALGTHDLRWIRDHAPDDGSVGVNDQTFERSCLGLWGPRARD
                     ILASVCADDLSDPAFRYLTARPITVAGLPVLALRVTYVGELGWELYGAMDDGLALWDA
                     LWAAGQPHGMVAGGYRAIDSLRLEKGYRVWSSDITPDDTPFQAGLGFAVRLDKEPGFI
                     GQDALRAADERPPDRRLSCLVLDEALTVAMGSEPIRVGGVVVGRVTSGGYGFTVAASI
                     AYGYLPVALVEPGTRCEVEVFGDWIGATVSAEPLHDPANARIRA"
BASE COUNT          449 a         1101 c         1105 g          436 t
ORIGIN      
        1 cacctggttc agccactgcc cgtagtcgct ccggctggcg cagacgtaga tccgatggtt
       61 ggtcgcatag ttgcggtcga ccgcgatccc catcaggcct gcctccccct gggcgcggac
      121 gtaggagacg acgttcgtgc gaagcagcgg cgcccccggc tcgccgctcg caaacacctt
      181 caggttgccg ccccgctcgg tgacgagcat cttcccgtcc ggcgtgaacg ccacgtccca
      241 tgggtacgac agcccgctct ggatgacctc ggagttcaac gtcgggccgc cggcagccgc
      301 cacggccggg aacagtccgc ccaggaacag gacggccatg accaagccga tgacccggct
      361 ccgcgtggtc ctcatcaccg gcactctagc ggcccggaag gatctcgcac ccacggagag
      421 gtaccggcgg cgctaggcaa aacggctacc ccgtcggcgg cgggctcgtg ggcggcgggc
      481 cagccggagg cggcccggcg ggcggcaggc catcgatcgg cgccgtgtcg tccatgccgc
      541 tcaggtcgtc cggtggcggc tcgttccggt tcgtgtacca cagcaccacg gccgcggctc
      601 ccgtccccag caagcccagg atgatgatcc agggcaggag tccgcctccc tcgtcatcag
      661 gtgtcgcgct cgggctgggg gaaggctcgg cggatcccga cgcgctggcg ctcggggtgg
      721 cagtcggcgt cgcggttggg ctcgccgtgg cggtgggtgc cggcgtggcc gtcggggcgg
      781 cagtcgccgt gggagccgcc gttgccgggg gtgccggcgt cgcggttggc gcggcagtcg
      841 gcgtttcgtc ctccgtctcg gtcggctcag ccgtggcggt cggaggcggc gcgggcgtcg
      901 ccgttggggc cggcgtcacg gccggtgtcg gggcgaccga aggcggcgtg ctgaccgccg
      961 gctcggacgg tgtcaacgac gagcccgggc agtcggtcct cagaccctcg gcgacgctct
     1021 ggaaggcccc gatggcgtcg gatgcgagct ggacgaggtc cagcgccgat gccacagcgc
     1081 ccgcgtcccc cgattcgatg gcgtcccgca ggttgctcga cgcggtgccg gtcgcctcga
     1141 gggccgcttg gaacgagacc cggaccacgg ggccgacctc ttcgtcggtg gcggcatcga
     1201 gggcttcctg ggcggtggcc ttcgtcgcgt caagcgaatc gagcgcggcc agggcggccg
     1261 cgttctcggg ggttcccgcc tgggcgacga gcgccggatc cagcgccgcg atcgtgtcga
     1321 gatcctgctg cagctcctcg ctgtagccga tcagcaggca gcccgggcct tcaacccctc
     1381 cgcatgcgac gccaaggacc atcagcgcca cgatggcagt cagggcggtg cggttcggga
     1441 gtcgtctcat gaaggaggaa gcgtagcgaa tgtccgctcg tggcgctcac atgccgacgg
     1501 aggtggcgga cgccgcgagc gccacatcgg tatcggtacc atcgcggcat gcagaaccgc
     1561 cgaccgcgca tcgtggtcac cgtccagtcg ccggagcacg cagccaacga ggaagtcgcc
     1621 tggcggaaga acgatcgcta cttcgaggcg gtgacgcgcc acgggggaga tgtcatcgtc
     1681 ctggacgagg acagtcccgc cgccgatcgc gtcaacgcat tcgcgtcgat ggacgggctc
     1741 ctgctgtccg gtggcgcaga tctcgacccg gcgctgtacg gcgaggaacg ccacccgacc
     1801 acggtcgtgg agcccgggcg tgacgcactc gaacaggcag ccttcgccgc cgccgaggag
     1861 cggggcgttc cggtattcgg tgtctgtcgc gggatgcagg cggtgaacgt gttccgtggc
     1921 ggctcgctgg tgcaggacat cgaaggtcat acgagcccgg cctatcccgc tccggaggcc
     1981 acgtcgcacg acctcgagat cgaggccggc acgcgcctgg ccgaggtgct cggcgagggc
     2041 ccggcgacgg tcaacaccta ccaccaccag gcggttcgac cggaccaggt gggccacggc
     2101 ctccggatca gcggctacag cccgcatcac gacggtgacc tggtggaagc cctggaggac
     2161 gcggacccgg accgctggct catggccgtc cagagccacc cggagcggac agagttcacc
     2221 tcgcccgagt tcgagcggct gtgggcttcg ttcctgggag cggccggcgc gttcgcggca
     2281 cggtaaccgc cgaccgaggg ctaggcgcga atccgggcgt tcgcggggtc gtggagcggc
     2341 tcggcgctga cggtcgcccc gatccagtcc ccaaagacct cgacctcgca ccgcgtccct
     2401 ggctcgacca gcgccacggg caggtagccg taggcgatgc tcgccgcgac cgtgaacccg
     2461 tagccgccac tcgtcacccg accgacgaca acgccaccga cgcggatcgg ctcgctgccc
     2521 atggccaccg tcagcgcctc atccaggacc aggcaggaca gtcgccggtc tggtggcctc
     2581 tcgtccgccg cgcgcagcgc gtcctggccg atgaaccccg gctccttgtc cagccgcacg
     2641 gcaaacccga gcccggcctg gaacggcgtg tcgtcagggg tgatgtccga tgaccagacc
     2701 cgatacccct tctccagcct gaggctgtcg atcgcgcggt agccaccggc caccatccca
     2761 tgcggctgcc cggctgccca cagcgcgtcc cacagggcga ggccgtcgtc catcgcgccg
     2821 tacagctccc accccagctc gccgacgtac gtgacccgga gggcaaggac cggcaatccg
     2881 gccaccgtga tcggtcgcgc ggtgaggtat cggaaggccg ggtccgacag gtcgtccgca
     2941 cagaccgatg ccaggatgtc gcgcgcccgc gggccccaga gcccaaggca cgagcgctcg
     3001 aaggtctggt cgttgacccc caccgacccg tcgtccggcg cgtggtcccg gatccagcgc
     3061 aggtcatggg tgccgagcgc cgtgccggtg a
//
LOCUS       DASYGU010000139         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1669268, whole genome shotgun sequence.
ACCESSION   DASYGU010000139 DASYGU010000000
VERSION     DASYGU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669268"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1308)
                     /locus_tag="VGO32_03685"
     CDS             complement(91..1308)
                     /locus_tag="VGO32_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEV7199883.1"
                     /translation="MFYVRRGQVPPKRHTQFRKPDGTLFAEELFGVEGFTGRASLLYH
                     HTPPTQTNRIEKVRDVALEAWSDAPHRHYLVNTRDVPPDGDAVSGRIPLFFNSDVVFG
                     VVRPAEAMTRFYRNGEADEMLFVHEGHGTLETIFGPIEYGPGDYLVIPIGTTYRVVPT
                     AGEPQRMLWVESPSAIVPPKRYRNEYGQLLEHSPYAERDIRPPAEMDAHVEAGEFVIE
                     VKTRGRITAYHYLHHPFDLVGWDGHLWPFAFNISDFEPITGRVHQPPPVHLTFQPRNY
                     VICSFVPRKFDYHPLAIPAPYNHSNINSDEVIYYVAGNFMSRRGVDISSFTVHPAGIP
                     HGPHPGTVEASIGKESTEELAVMIDTFHPLHLTRQAGELEDPAYPFSWLPPTDADAEA
                     RELSDRGPEAFPD"
     gene            complement(1351..2202)
                     /locus_tag="VGO32_03690"
     CDS             complement(1351..2202)
                     /locus_tag="VGO32_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV7199884.1"
                     /translation="MSVNPEDLPAWAEPFAAADQLARRAGLRRIDRSWAWGGASGKGV
                     TVAIVDSGVEGGHPWIGDRLVESVAVRLDKRGEPEVVPDDPIDLYGHGTACAGIVLDM
                     APDVDLISVRVLNDDLKGKGAAFVEGLSWAIQRGVQVANLSLSSKSEALYPVFHELAD
                     EAYFRNVILVSAANNVNVASYPSLFSSVVSVAAHAEPDPWRLYYNPNPPVEFGAWGVD
                     VPIAWKDGTETVATGNSFAAPHVAGMAARILSKHPGLTPFEVKAILTAVSDNSRTGRR
                     RASPQRG"
     gene            complement(2207..3001)
                     /locus_tag="VGO32_03695"
     CDS             complement(2207..3001)
                     /locus_tag="VGO32_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7199885.1"
                     /translation="MTTRPSSKSLDQALAIIRAGAERAAVAERLHPDSEQRLLQSIVD
                     ATVRLFDAEAASIAVFERDPDRLEFRVAAGPRGAGAIGKSVKPSQGIAGFVFSSGQAL
                     ALSDVASDPRFDRKTAEKTGYVPRSIAAVPLADGEGTHGVLQVLDKRSTATFDLRDME
                     LIGVFAAQAATAIAVTRAQRELPLLLRSAFARASGETLTDDQLDALVSASSTDLDLDD
                     DTPFWSLADQVAQLRGLGESELSLVSEILAALVAHAASRRARSRYR"
     gene            complement(3063..>3334)
                     /locus_tag="VGO32_03700"
     CDS             complement(3063..>3334)
                     /locus_tag="VGO32_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199886.1"
                     /translation="TKAKTKARRREDEDTEGHAHTRVKTKAEQPAKTKARRREDGDTE
                     GHAHTRVKTKVNTKAKTKARRREDEDTEGHAHTRVKTKAASSKTR"
BASE COUNT          494 a         1079 c         1197 g          564 t
ORIGIN      
        1 agggcaaccc acagtgcagc ccctgtcaca tcgcgagctc tcagtctgct gccggcaagg
       61 ttcatggcgg gcctatctgc ggtgccgcga tcagtccgga aaggcctcgg gtccgcggtc
      121 ggacagctcg cgggcctcgg cgtccgcatc ggtcggtggc agccagctga acggataggc
      181 ggggtcttcg agctcgccgg cctggcgggt gaggtggagc ggatggaagg tgtcgatcat
      241 caccgccagc tcctccgtcg actccttgcc gatggacgcc tccaccgtgc cggggtgcgg
      301 gccatgggga atcccggccg gatggaccgt gaacgagctg atatccaccc cccgccggct
      361 catgaagttg ccggccacgt agtagatgac ctcgtcggaa ttgatgttcg agtggttgta
      421 cggcgcgggg atggcgagtg ggtggtagtc gaacttgcgg ggcacaaagc tgcagatgac
      481 gtagttgcgc ggctggaagg tgaggtggac cggaggtggc tggtggacgc gtccggtgat
      541 cggctcgaag tcggagatgt tgaaggcgaa cggccacagg tgcccatccc agcccaccag
      601 gtcaaacgga tggtgcaggt agtgataggc cgtgatccgg ccgcgggttt tgacttcgat
      661 cacgaactca ccggcctcga catgagcgtc catctcagcc ggaggccgga tgtcgcgctc
      721 cgcgtacggg gagtgctcga ggagctggcc gtactcgttg cggtagcgct tgggcgggac
      781 gatggccgag ggtgactcaa cccacagcat ccgctgcggc tcgcccgcgg tcgggacgac
      841 ccggtaggtg gtgccaatcg ggatgacgag gtagtcgcct ggcccgtatt caatcggtcc
      901 gaagatcgtc tccagcgtgc cgtgcccctc gtggacgaag agcatctcgt ccgcttcccc
      961 attgcggtag aagcgggtca tcgcctcagc cggccggacg accccgaaca cgacgtcgct
     1021 attgaagaac agcgggatgc ggcccgatac cgcgtcgccg tcgggcggga cgtccctggt
     1081 gttcaccagg taatggcgat gcggcgcgtc ggaccacgcc tcgagcgcca cgtcgcggac
     1141 cttctcgatc cgattggtct gggtcggcgg ggtgtggtgg tagagcagcg acgcgcgacc
     1201 ggtgaacccc tcaaccccga acagctcctc cgcgaagagg gtgccgtcgg gctttcggaa
     1261 ctgcgtgtgg cgcttgggcg gcacctggcc acggcggacg tagaacacga ggtcgccatt
     1321 ctaggccgcg cctgaggcgc agccgaccgg tcagccccgc tgcggtgacg cccggcgacg
     1381 tccggttcgc gagttgtccg acacggcggt gaggatggcc ttcacctcga acggggtgag
     1441 gcccgggtgc ttggacagga tgcgggcggc catacccgcc acgtgcggcg cggcgaacga
     1501 gttcccggtg gccaccgtct cggtcccgtc cttccatgcg atcggaacgt ccacgcccca
     1561 cgccccgaac tcgactggcg ggttggggtt gtaatagagc cgccaggggt cgggctccgc
     1621 gtgggccgcg acggatacga cgctgctgaa cagggatgga taggacgcaa cgttcacgtt
     1681 gttcgcggcg ctgaccagga tcacgttcct gaagtaggcc tcgtccgcga gctcgtggaa
     1741 aaccgggtag agcgcctcgc tcttggacga caagctcagg ttggcgacct gcacgccgcg
     1801 ttggatggcc cacgaaagcc cttcgacgaa tgcggcgcct tttcccttca ggtcgtcgtt
     1861 cagcacgcgg acggagatga ggtccacgtc cggggccatg tccaggacga tcccggcgca
     1921 tgcggtcccg tgcccataca ggtcgatggg atcgtccggc acgacctcgg gctctccccg
     1981 cttgtccagc cgcacggcca cgctctcgac cagccggtcg ccgatccatg gatgcccgcc
     2041 ttccacgccg ctgtccacga tcgcgaccgt cacgcctttc ccgctggcgc cgccccatgc
     2101 ccacgaccga tcgatccgtc ggagcccggc gcggcgcgcc agctggtcgg cggcggcgaa
     2161 cggctcggcc caggccggga ggtcctccgg gttgaccgac atggtgtcag cggtagcggg
     2221 aacgcgcacg ccgggaggcg gcatgggcaa ccaacgcggc caggatctcg ctgaccaggc
     2281 tgagctccga ctcgccgagc ccccgcagct gggcaacctg gtcggcgagc gaccagaacg
     2341 gggtgtcgtc gtcgaggtcc aggtcggtcg acgacgcact caccagcgca tcgagctggt
     2401 catcggtcag ggtctcgccg gaggcgcggg cgaatgccga tcgcaggagc aggggcagct
     2461 cacgctgggc ccgggtcacg gcgatcgcgg tcgctgcctg ggcggcgaag acgccgatga
     2521 gctccatgtc gcgcaggtcg aaggtcgcgg tgctccgctt gtccagcacc tggaggacgc
     2581 cgtgggtgcc ctcgccgtcg gccaatggca ccgcggcgat ggaccggggc acgtagccgg
     2641 tcttttccgc ggtcttccga tcgaagcgcg gatcggaggc cacgtcggac agggccagtg
     2701 cctgcccgga cgagaacacg aacccggcga ttccttggct gggcttgacg ctcttgccga
     2761 tcgctcccgc accacgcgga ccggccgcca cgcgaaactc gagccgatcc ggatcgcgct
     2821 cgaacacggc aatcgaggcg gcctctgcat cgaacaggcg gacggtggcg tccacgatcg
     2881 actggagcag gcgctgctcg ctgtccgggt gaagtcgctc ggccaccgca gcccgttcgg
     2941 cgccggcccg gatgatggcg agcgcttggt ccagcgactt cgacgacggg cgggtggtca
     3001 tcgaggcata gcgtaaggcg gcggggagga ccccgccgcc ttacctgttc gggaagcgga
     3061 cgttatcgcg tcttggagga cgcggccttg gtcttgacgc gggtgtgggc gtggccttcg
     3121 gtgtcctcgt cctcacggcg ccgggccttg gtcttcgcct tggtgttgac cttggtcttg
     3181 acgcgggtgt gggcgtggcc ctcggtgtcg ccatcttctc ggcgccgggc cttggtcttc
     3241 gccggctgct cggccttggt cttgacgcgg gtgtgggcgt ggccttcggt gtcctcgtcc
     3301 tcacggcgcc gggccttggt cttcgccttg gtgt
//
LOCUS       DASYGU010000140         8606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_589167, whole genome shotgun sequence.
ACCESSION   DASYGU010000140 DASYGU010000000
VERSION     DASYGU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589167"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGO32_03705"
     CDS             complement(<1..582)
                     /locus_tag="VGO32_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7199887.1"
                     /translation="MTAESTAERSGQAARFAEELGTAVSAAVVGADQPLRLVAVALLA
                     DGHALIEDVPGVGKTLLARAVARAIGLTFARIQGTPDLLPGDITGSSILEGNAFRFVP
                     GPIFNQVVLVDEINRATPRTQSALLEAMQERQASVDGVTRPLPEPFIVLATQNPIELE
                     GTFALPEAQLDRFLLKIGMGYPSTASEQAIARRY"
     gene            complement(587..1246)
                     /locus_tag="VGO32_03710"
     CDS             complement(587..1246)
                     /locus_tag="VGO32_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV7199888.1"
                     /translation="MYVVVIGGGNVGYYLTRELLNAGHEVVMIEKNASRARQIADELG
                     SIVVPNDGCEGRYQEQAGMGRADVVAAVTGDDEDNLVACQVAKMHFNVPRAIARVNNP
                     KNEALFRQLAIDDTVSPTRTILGVIEHEIPIHDLLHLAELEGGDLEIVEAQITADSPV
                     VGRELRDLPLPDGTSVAVILRGSRAVPARPETKLADGDRMLVVTSAEGEPELRRLVIG
                     D"
     gene            complement(1249..1749)
                     /locus_tag="VGO32_03715"
     CDS             complement(1249..1749)
                     /locus_tag="VGO32_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV7199889.1"
                     /translation="MRVIIIGCGRVGSATAVQLDKRGDHVTVIDLDAHAFEKLPSDYS
                     GITIRGNGADEDILRSADAQMADIVMALTEGDNRNVMAAQLAKHVFGVPRVVAKINDP
                     LRADAYRTLGLETICRTTILSGALVVAAVDGAAATNGMVEEPTAEPMRGAPPHGTQPV
                     PAAEEQ"
     gene            complement(1746..2249)
                     /locus_tag="VGO32_03720"
     CDS             complement(1746..2249)
                     /locus_tag="VGO32_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7199890.1"
                     /translation="MFRRRPSDQDQALLPYRRILVPLAGAEADDDALRLAALLLTAPG
                     GDRAEPGEVVLVHVIEVGFERTLDTEDERATNFADEVLGRGAEFCEARGIPHRVGMIQ
                     ARAAGPAIVDEAVASSADLIVMGLRYKRRFGGSWDAGRTVPYVMRNSKAPVWCLRART
                     EELALSP"
     gene            complement(2273..2713)
                     /locus_tag="VGO32_03725"
     CDS             complement(2273..2713)
                     /locus_tag="VGO32_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEV7199891.1"
                     /translation="MSAAVSGPASNGSVLVVRLPDAMPAERAEAAIEAAFDPGSAAAL
                     ATDLPGDLDWASSGVICVFLGRREDGGWSLAIQGASLVDGELQVRARETRPAPGVDRP
                     GPSFAADCATIDRPALPVGTLPARADDTVTDEFIVAGDLEVPAP"
     gene            complement(2823..3554)
                     /locus_tag="VGO32_03730"
     CDS             complement(2823..3554)
                     /locus_tag="VGO32_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV7199892.1"
                     /translation="MRLAVLSDIHANLVALDAVLADLAGLAAVDQVWTCGDNVGYGPQ
                     PNEVVARLRERNARAVMGNHDGAAVGTVDVAWFNDAAAAAIRWTSDVLSGESRDYLAG
                     LPERLVDGQITAVHGSPRDPIWEYITSPAVAAASLSAFDTPICLFGHTHFPVSYVQDD
                     DGVREMVGSAGPATPLPGGRLMLNPGSVGQPRDGDPDSSYLVLDLDARTVEFRRVAYD
                     IAATQALMRAADLPAWLVERLSHGR"
     gene            3834..4298
                     /locus_tag="VGO32_03735"
     CDS             3834..4298
                     /locus_tag="VGO32_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199893.1"
                     /translation="MAVLLSEISPPRFAHDSEAELARVLDFYQVAWRYEPDVFPISWS
                     PSGAVAESFAPDFYLPELEIYVELTTLRQSLVRKKNRKLRLLRQLYPEVRIKLLYMRD
                     FQALMVKYGRVDFLLEMNGTNGHGNSNGTGAGKRANGVATPGRTPASEVATG"
     gene            4295..4873
                     /gene="hpt"
                     /locus_tag="VGO32_03740"
     CDS             4295..4873
                     /gene="hpt"
                     /locus_tag="VGO32_03740"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV7199894.1"
                     /translation="MSRTSVATIHADVEEILLTAEQIQARVAELGVQLTADYEGRDPV
                     LISVLKGSIVFLADLVRSMDLALSIDIMEVSSYGAATESSGQVRILKDLSNPIEGRHI
                     IVVEDIIDTGLTLNYLLRYLREKGPASLTICCLLDKPARRLTEIPIDYVGFTIPDRFV
                     VGYCLDYGERYRNLAYVGVLRPSVYGLDNPEG"
     gene            4877..5311
                     /locus_tag="VGO32_03745"
     CDS             4877..5311
                     /locus_tag="VGO32_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199895.1"
                     /translation="MDDLGRPRRFRVPILIGSLLMVGAAFLPWWRAGGEQVSGVTLPA
                     SDGIGLEGPGMVIFVAAILALALLDIGYARGRWGFALDAPGIYLLLGAAAAAALVWRG
                     WELWSVAYLPLPQTSPGLILAVVGVGLFLYGAGTGIGSRRPL"
     gene            complement(5491..6201)
                     /locus_tag="VGO32_03750"
     CDS             complement(5491..6201)
                     /locus_tag="VGO32_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199896.1"
                     /translation="MTHLLELEDVHTYYGNIHALKGVSLTVDEGEIVTLIGSNGAGKS
                     TTLRTISGIARAREGAVRFKGQDIHTRKPHEIVGLGIAQAPEGRGVFARMSVYENLLM
                     GAFSRKGSFTEDVDKVYALFPRLAERRSQKAGTLSGGEQQMLAIGRALMAKPTVLLLD
                     EPSMGLSPILTEQIFEIIRDINAQGNTVLLVEQNAQMALNLAGRGYVLQTGQIILADT
                     AANLAANQQVRQAYLGEI"
     gene            complement(6198..6998)
                     /locus_tag="VGO32_03755"
     CDS             complement(6198..6998)
                     /locus_tag="VGO32_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7199897.1"
                     /translation="MTDQPAASGTLLQATKVTKTFEGLVANRDITFDIPRQSIVSIIG
                     PNGAGKTTFFNQLSGVYHPTEGTITLDGEDITDMPPHAVAKLGVARTYQNIRLFKDMT
                     VLANVSVGQHVRTHGAWFASIFRLPGIRREEAGIEKKSFEMLELVGLRRRVADELAKN
                     LPYGDQRRLEVARALASDPVLLLLDEPTAGMNPGETRQMTEFIARLQRDLNLTILLIE
                     HDMKVVMGVSERITVLDYGEKIAEGTPAEIRANPRVIEAYLGKQATAS"
     gene            complement(6995..>8606)
                     /locus_tag="VGO32_03760"
     CDS             complement(6995..>8606)
                     /locus_tag="VGO32_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7199898.1"
                     /translation="YLTLGRLMLFAPPLIAGFWIGGKVTGASRQVASGLVTGLGAGIL
                     FAIGFLVASALSPGIRDVLLRITPAVLHFIGFGLDPLPGAALNVAVATGLGLLGAGGR
                     AVPVRIRTPLVAGVGAVVLLSMLEPFLRPRLLEFGLIELARWIYADHGLTLLATVATF
                     LIGAGIAAALPVVRATTRRRLAAADPGPRRLVGWVAVLVLIAILYTLPVIAGSFLSQV
                     LVLVGLYVLLGLGLNIVVGFAGLLDLGYVAFYAVGAYVTALLTSPTSSLGLGLNFWVA
                     LPIVVIVAAVVGLFVGAPVLRLRGDYLAIVTLGFGEIARLLFLSEALRPWFGGVQGIL
                     GVPPMIGTTGSYTLPIIGEVSGPAATYYPLVAFCALAAFFTWRLKFSRTGRAWNAMRE
                     DEDVAQATGVNIVNHKLLAFALGASVGCIGGAVYVTHLESVFPGAFNIFVSITALAVV
                     IVGGMGSIPGVILGALALVGLPELLREFAEFKLLVYGAILVTMMLWRPEGLFPDKARR
                     RELHEGEGDEEANPENPEPAATAAPSQA"
BASE COUNT         1344 a         2917 c         3025 g         1320 t
ORIGIN      
        1 gtagcggcgg gcgatggcct gctccgacgc cgtgctcgga taccccatcc caatcttcag
       61 caggaaccgg tcgagctgcg cctcgggcag ggcgaacgtg ccctccagct cgatcggatt
      121 ctgggtggcc agcacgatga acggctcggg cagcggccgg gtgacgccgt cgaccgacgc
      181 ctgtcgctcc tgcatggctt cgaggagggc ggactgggtg cggggcgtgg cacggttgat
      241 ctcgtccacc agcaccacct ggttgaagat cgggcccggg acgaagcgaa acgcgttgcc
      301 ctcgaggatg ctgcttccgg tgatgtcgcc tggcagcagg tccggcgtgc cctggatccg
      361 ggcgaaggtc agaccgatgg cgcgcgcgac ggcgcgcgcg agcagcgtct tgcccacccc
      421 gggtacgtcc tcgatcaggg cgtggccgtc ggccagcagc gcgaccgcga ccaggcggag
      481 gggttggtcc gcgccaacga cggccgccga taccgcggtg ccgagctcct cggcgaagcg
      541 ggccgcctgt ccgctgcgct cggccgtcga ttcggccgtc atcggcctag tcgccgatca
      601 cgagccgtcg cagctcgggc tcaccttcgg cggaggtcac caccagcatc cggtcgccat
      661 ccgccagctt ggtctcgggg cgggccggga ccgcgcgcga cccgcgcagg atgaccgcca
      721 ccgacgtgcc gtcgggcagc ggcaggtcgc gcagctcgcg tccgacgacg ggcgagtcgg
      781 cggtgatctg ggcttccacg atctcgagat cgccgccttc cagctccgcc aggtggagga
      841 gatcgtggat cgggatctcg tgctcgatca cgcccaggat cgtccgggtc gggctcaccg
      901 tgtcgtcgat ggccagctgc cggaagagcg cctcgttctt cgggttgttg acccgggcga
      961 tggcccgcgg gacgttgaag tgcatctttg ccacctggca ggcgaccagg ttgtcttcgt
     1021 cgtcgccggt caccgcggcc acgacatccg cccggcccat gccggcctgt tcctggtatc
     1081 ggccctcaca tccgtcatta gggacgacga tgcttcccag ttcatcggcg atctggcggg
     1141 cgcggctggc gttcttctcg atcatcacca cttcgtggcc cgcgttgagc agctcgcgcg
     1201 tcaggtagta gccgacgttt ccgccgccga tgacgaccac gtacacggct actgctcctc
     1261 ggccgccggg accggctggg tgccgtgtgg cggggcgccg cgcattggtt cggcggtcgg
     1321 ctcctcgacc attccgttgg tcgcggccgc gccgtccacc gccgcgacga ccagggcgcc
     1381 ggacaggatg gtggtccggc agatcgtttc caggcccagc gtccggtagg catcggcccg
     1441 cagcggatcg ttgatcttgg ccacgacgcg ggggacgccg aacacatgct tggccagctg
     1501 ggcggccatc acgttgcggt tgtccccttc ggtcagggcc atgacgatgt ccgccatctg
     1561 ggcatctgct gagcggagga tgtcctcgtc cgcgccgttg ccccggatcg tgatcccgct
     1621 gtagtcgctc ggcagcttct cgaacgcgtg cgcgtccagg tcgatgaccg tgacgtggtc
     1681 gccgcgcttg tcgagctgga cggcggtggc cgatccgacc cgcccgcagc cgatgatgat
     1741 gactctcatg gggacagtgc cagctcctcg gttcgcgccc ggaggcacca gaccggcgcc
     1801 ttcgaattcc gcatgacata ggggaccgta cgaccggcat cccagcttcc gccgaaccgg
     1861 cgcttatagc gtagtcccat cacgatcagg tcggcgctcg aggccaccgc ctcgtccacg
     1921 atggccgggc cggcggcacg ggcctggatc atcccgaccc gatgggggat gccccgcgcc
     1981 tcgcagaact cggcgccgcg accgaggacc tcgtcggcga aattcgtcgc ccgttcgtcc
     2041 tcggtgtcca gggtccgctc gaatccgacc tcgatgacgt ggaccaggac gacctcgccg
     2101 ggttcagcgc gatcgccgcc gggcgctgtc agcaggaggg ccgcgaggcg caatgcgtcg
     2161 tcatcggcct cggcccctgc caggggtacg aggattcgcc gataggggag gagtgcctgg
     2221 tcctgatccg aggggcggcg gcggaacatg cggccgatat cctagccgtc cgtcacgggg
     2281 ctgggacctc gaggtcgccg gcaacgatga actcgtcggt gaccgtgtcg tccgcccgtg
     2341 ccgggagggt gccgacgggc agggcggggc gatcgatggt ggcgcagtcg gcggcaaacg
     2401 acggaccggg tcgatccacg cccggggccg gacgcgtttc gcgggcccgg acctggagct
     2461 cgccgtcgac caggctcgcg ccctggatgg ccagcgacca gccgccgtcc tcgcgacgcc
     2521 cgaggaagac gcagatcaca ccgctggatg cccagtccag gtctcctggc aggtcggtcg
     2581 cgagggcggc cgccgacccg ggatcgaagg cggcctcgat ggccgcttcc gcccgctcag
     2641 cgggcatggc atccggcagc cggacgacga gaaccgaccc gttggacgcg ggacccgaga
     2701 cggccgcgga catcgtgggc agggcggctg ccgacgggga agccgagccg gacggcgcac
     2761 cgcacgccgc cagggcgaaa acgagaccca gcgccgttct gccccggcgc gcgggaccgc
     2821 ggtcagcgac cgtggctgag ccgctccacg agccatgccg gcaggtctgc cgcgcgcatg
     2881 agtgcctgtg tcgccgcgat gtcgtaggcc acccgacgga attccacggt ccgcgcgtcg
     2941 agatccagca cgaggtatga gctgtccggg tccccgtcgc gcggctgacc gacgctgccc
     3001 gggttcaaca tcaaccgacc gcccgggagc ggggtcgcgg gcccggccga gccgaccatc
     3061 tcccggacgc cgtcgtcgtc ctggacgtag ctgaccggga agtgggtgtg cccgaacagg
     3121 cagatcggcg tgtcgaaggc gctgaggctg gcggcggcca ccgccggcga ggtgatgtac
     3181 tcccagatcg ggtcccgcgg tgagccgtgc acggcggtga tctggccgtc gacgagacgc
     3241 tcgggcaggc cggccaggta gtcgcgcgac tcgcccgaga ggacgtccga cgtccagcgg
     3301 atggccgccg ccgcggcgtc gttgaaccac gccacgtcaa ccgtgccaac cgccgccccg
     3361 tcgtggttgc ccatcaccgc ccgcgcgttc cgctcgcgga gtcgggcgac gacctcgttc
     3421 ggctgcgggc cgtatccgac gttgtcaccg caggtccaca cctggtcgac cgccgcgagg
     3481 ccggccaggt cggcgaggac ggcgtccagc gcgaccaggt tggcgtggat gtccgacagg
     3541 acggcaaggc gcatgggcgg ccgagtgtaa cgagaaatgc tccctcggcg cctttcggtc
     3601 ttcccggaga tgagcggttt cgcggccggc tctcgcatcg gccccgcaca ttcccggtta
     3661 cccatactcg gcaccgcgga agcaggacca acagtgtcct gcgcagccgg agatcaggca
     3721 agtgcaaata tccacaaacc gtgcgccgag tcgccgacct gtggataact tcgagggcta
     3781 tactcgtctc cgatggcgcc tcaccccttc ggggcgagta cgatcgggca ccggtggcgg
     3841 ttctgctgag cgagatttcg ccgccgcgct tcgctcacga cagcgaagcc gaattggccc
     3901 gggtccttga cttctaccag gtggcatggc gctatgagcc cgacgtcttc ccgatctcct
     3961 ggagcccatc cggagcggtg gccgaatcat tcgcgccgga cttctacctg ccggagctgg
     4021 agatctacgt ggagctgacc acgctgcgcc agtccctcgt gcgcaagaag aaccggaagc
     4081 tgcggctcct tcgccagctc taccccgagg tgcggatcaa gctgctgtac atgcgggact
     4141 tccaggccct gatggtgaaa tatggccggg tggacttcct gctcgagatg aacggcacga
     4201 acggccacgg gaacagcaac gggacgggcg ccgggaagcg ggcgaacggc gtcgccacgc
     4261 ccggccgaac cccggcctcg gaggtcgcca ccggatgagc aggacctcgg tcgcgaccat
     4321 tcatgccgac gtggaggaga tcctgctcac cgcggagcag atccaggccc gggtggccga
     4381 gctcggcgtt cagctgacag cggactacga gggacgcgat ccggtgctga tcagcgtcct
     4441 gaagggttcg atcgtgttcc tcgccgatct cgtccggagc atggatctgg ccctgtcgat
     4501 cgacatcatg gaggtgagct cgtacggagc cgccaccgag agcagcggcc aggttcggat
     4561 cctcaaggac ctgtcgaacc cgatcgaggg tcgccacatc atcgtggtcg aagacatcat
     4621 cgacaccggc ctgaccctga actacctgct ccgctacctg cgcgagaagg ggccggcctc
     4681 gctgaccatc tgctgcctcc tggacaagcc cgctcgtcgg ctgaccgaga tcccgatcga
     4741 ctacgtcggg ttcacgattc ccgatcggtt cgtggtcggc tactgcctcg actacggcga
     4801 gcgctaccgc aacctggcct acgtcggcgt cctgcggcca tcggtctacg ggctcgacaa
     4861 cccggagggt tgagcggtgg acgacctcgg tcggccgcgt cgcttccgcg ttcccatcct
     4921 gatcggaagc ctgctgatgg tgggcgccgc cttcctgccg tggtggcgcg ccggcggtga
     4981 acaggtgagc ggggtcaccc tccccgcctc cgacgggatc ggcctcgagg gacccggaat
     5041 ggtcatcttc gtcgccgcca tcctcgccct cgccctgctc gacatcggct acgcccgcgg
     5101 gcggtggggc ttcgcgctcg acgcacccgg gatctatctg ctgctgggcg ccgccgccgc
     5161 cgccgccctc gtgtggcgcg gctgggagct gtggtcggtc gcctacctgc cgctgccgca
     5221 gacctcgccc ggcctcattc tggcggtggt cggtgtcggg ctgttcctgt acggggccgg
     5281 gaccgggatt gggagccgcc gcccgctcta gcggccggaa aaccgcgcca tggcgagtca
     5341 gggcgccggc aggaggcgcg ggctgaggat gatccagccc accacgacca cgatgaggac
     5401 agcgaccacc agcgccagcg ggcggatgtc gggtcggtag gggggcttcg ccatcgtccc
     5461 ggtgcggcgc gacggccggc ggccccaggc ctagatctcg cccaggtagg cctggcgaac
     5521 ctgctggttg gcggcgaggt tggccgccgt gtcggcgagg atgatctggc ccgtctgcag
     5581 gacatagccg cggcccgcca ggttcagcgc catctgggcg ttctgctcga ccagcaggac
     5641 cgtgttgccc tgggcattga tgtcccggat gatctcgaag atctgctcgg tcaggatcgg
     5701 tgacaggccc atcgacggct cgtccaggag caggacggtg ggcttggcca tcagcgctcg
     5761 accgatcgcc agcatctgct gctcgccacc cgacagcgtc ccggccttct ggctccgcct
     5821 ctcggccagg cgcgggaaga gggcatagac cttgtccacg tcctcggtaa atgacccctt
     5881 ccggctgaac gcccccatca gcaggttctc gtacacgctc atccgggcga acacgccacg
     5941 tccctcgggc gcctgggcga tgcccagccc cacgatctcg tgcggcttgc gggtgtggat
     6001 gtcctggcct ttgaagcgca ccgcaccttc gcgggcgcga gcgatgccac tgatcgtgcg
     6061 cagggtggtg gacttgccgg cgccattcga gccgatgagg gtcacgatct cgccttcgtc
     6121 cacggtcagc gacacgcctt tcagagcgtg gatgttgccg tagtaggtgt ggacgtcctc
     6181 gagctcgagc aggtgggtca tgaggcggtt gcctgcttgc caaggtaggc ctcgatgacc
     6241 cgcgggttgg cccggatctc ggccggtgtt ccctccgcga tcttctcgcc gtagtcgagc
     6301 acggtgatcc gctcgctgac gcccatcacg accttcatgt cgtgctcgat gaggaggatc
     6361 gtcaggttca ggtcccgctg caggcgggcg atgaactcgg tcatttggcg cgtctcaccg
     6421 gggttcatgc cggcagtcgg ctcatcgagc aggaggagca ctgggtccga ggccagggcg
     6481 cgggcgacct cgagccggcg ctggtcaccg tagggcaggt tcttggccag ctcatccgcc
     6541 acccgacggc ggagaccgac cagctcgagc atttcgaacg acttcttctc gatccccgcc
     6601 tcctcgcgcc ggatgccggg gagccggaag atggacgcga accaggctcc atgggtccga
     6661 acgtgctggc cgaccgagac gttggccagg acggtcatgt ccttgaacag acgaatgttc
     6721 tggtaggtcc gagccacgcc cagcttggca accgcgtggg gcggcatgtc ggtgatgtcc
     6781 tcgccgtcga gggtgatggt cccctcggtg gggtgataga ccccgctcag ctggttgaaa
     6841 aaggtggtct tgccggcgcc gttggggccg atgatcgaca cgatcgactg gcgagggata
     6901 tcgaacgtga tgtcccggtt cgcgacgagg ccctcgaagg tcttggtgac cttcgtcgcc
     6961 tggaggaggg tgcctgatgc cgcgggctgg tcggtcatgc ctggctcggc gcggcggtgg
     7021 ccgcgggctc cggattctcg ggattcgctt cctcgtcccc ttcgccttcg tgcagctcgc
     7081 ggcggcgtgc cttatcgggg aacaggccct cgggccgcca cagcatcatg gtgaccagga
     7141 tcgcgccgta gaccaggagc ttgaactcgg cgaactcgcg gagcagctcg ggcagtccga
     7201 cgagcgccag cgcacccagg atcacgcccg gaatgctgcc catcccgccg acgatcacga
     7261 ccgcgagagc ggtgatcgac acgaagatgt tgaacgcgcc ggggaagacc gactcgaggt
     7321 gggtgacgta caccgcgccg ccgatgcagc ccacggaggc cccgagggcg aaggcgagga
     7381 gcttgtggtt gacgatgttc acgccggtcg cctgtgccac gtcctcgtcc tcccgcatcg
     7441 cgttccaggc gcggccggtt cgtgagaact tcagccgcca cgtgaagaac gccgcgaggg
     7501 cgcagaacgc gacgagcggg tagtaggtgg ccgccggtcc gctgacctcc ccgatgatgg
     7561 gaagggtgta ggagccagtg gtgccgatca tgggcgggac tccgaggatg ccctgcacgc
     7621 cgccgaacca cggccggagg gcctccgaca ggaacaggag gcgggcaatc tcaccaaagc
     7681 ccagggtcac gatggccagg tagtcgccgc gcagccggag gactggggcg ccaacaaaga
     7741 gcccaacgac ggcggccacg atcaccacga tcgggagcgc cacccagaag ttgaggccca
     7801 ggcccagcga agacgttggc gaggtcagca gcgcggtgac atacgctccc accgcgtaga
     7861 acgccacgta gccaaggtcc agaaggccgg cgaagccgac cacgatgttc aggcccaggc
     7921 ccaggaggac atacaggccg accagcacga ggacctggct caggaaggag ccggcgatca
     7981 ccggcagggt gtagaggatg gcaatgagga ccagcactgc gacccacccg acgagccggc
     8041 gcggacccgg atcagcggca gccagccgcc gccgagtggt ggcccggacg acgggaagcg
     8101 cggccgctat gccggcgccg atgaggaaag tcgccaccgt ggccaggagg gtgaggccgt
     8161 ggtcggcgta gatccacctc gcgagctcga tcagaccgaa ttccaggagg cgcggccgga
     8221 ggaacggctc cagcatgctc agcaggacga ccgcgcccac accggccacc agcggcgtcc
     8281 ggatgcgtac cggaaccgca cgcccgccgg ctccgaggag gccgaggccg gtcgccacgg
     8341 ccacgttgag cgccgcacca ggcaggggat cgagaccgaa gccgatgaag tggaggaccg
     8401 caggcgtgat ccggaggagg acgtcccgga tcccgggtga gagggcgctg gcgacgagga
     8461 agccgatcgc gaacaggatt ccggccccca gccccgttac caggccggag gcgacctgcc
     8521 gtgaggcgcc ggtgaccttc cccccgatcc agaagcccgc gatcagcggg ggcgcgaaca
     8581 gcatcagccg gcccagggtg agatag
//
LOCUS       DASYGU010000141         9356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_74428, whole genome shotgun sequence.
ACCESSION   DASYGU010000141 DASYGU010000000
VERSION     DASYGU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74428"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGO32_03765"
     CDS             complement(<1..305)
                     /locus_tag="VGO32_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7199899.1"
                     /translation="MTAQTDVLIHARELTKRFGDFTAVDGVDFDVHVGEAFGFLGPNG
                     AGKTSTMRMIACISPVTDGALSVVGMNPSKDGTRIKSRIGVVPQEDNLDVELTVMENL
                     "
     gene            393..2588
                     /locus_tag="VGO32_03770"
     CDS             393..2588
                     /locus_tag="VGO32_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEV7199900.1"
                     /translation="MSPVAGQFLLDGSALGFEPGDTVAVAVVRAAQHPGHGGTLCLAG
                     DCGNCVAEVEGIAYVRTCLTPAADGMAVTRHPTDGPPRWHAEPRETEVPVAHDETDVA
                     VIGGGDAGRAAATAEEQAGRRVLVLDGPAGDEVVAVYAGPTVIVRRHGAMVHIRATEV
                     VVATGTAETQPVGPGSRLIGLLTSRAAQQLHAAGVDLGRAVAVGPVPAGVPCTALRGS
                     LLRLEGDSRVSAVVTRDAEGENRVECDTVILDLGRSPRDMLSRMAGDLPVRVVGSAAE
                     THPLPPPPTAGVVCPCVGTTVDDLEGVWSRGFRELELIKRASLAGTGTCQGSVCMPHV
                     LSWIAARTGEAPPPFTARPASRQITLGEAAADVHLDAFRRTPLHDEHLALGARMDRFG
                     GWWRPWHYGDHLAEYRAVREAVSLGDVSTLGKFIVAGQDTVEFLQRLYPTDVSTIRPG
                     RSRYALLLNERGHVIDDGMICRETEDRFVLTFTTGGAGNAEMWMRDWLETWGLQVHLM
                     DRTMSLAAINVTGPRAAELLARVGLTDPLRFLQHAHVEVAGIPAHVMRLSFTGEASFE
                     IHHPVDRSVDLWRGLMAMGGDMGIRPHGLQALFALRLEKGHVIIGMDTELDSTPRRLG
                     MDWAVNMDKRDFLGRDALARTAALPDHRRLVGLTMDGPAPTEGSPIWSDGDVIGHVTS
                     TFASPTLGHAVMLGWQKRTPFPEQVEVDGRTATVGAPPFYDPEGARARA"
     gene            2575..2946
                     /locus_tag="VGO32_03775"
     CDS             2575..2946
                     /locus_tag="VGO32_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199901.1"
                     /translation="MRAPEPLPATRVVAAPAVLDGIATDGGITLRLAVDEALVLGGGA
                     VVDDEAALVEDEAGFVGWWLTPDELTEVAHHLEWTIPAERPALAQGLVAGVPARLWLT
                     EERSLLLVSGAYAHELVERLP"
     gene            2943..4151
                     /locus_tag="VGO32_03780"
     CDS             2943..4151
                     /locus_tag="VGO32_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7199902.1"
                     /translation="MNEFVETILDLPWHEPKGSYDVVIVGGGGHGLATAYYLATRHGI
                     TNVAVVEANYIGSGNSGRNTTIIRANYGIPESVRFYQHSLELYATLEDETECWIMHST
                     KGLLWLAHTEWGMRAERARTLMNQACGAETVMVTPEEAKALCPALDLSGGGRYPILGA
                     SYHPGAATARHDRVVWAYAQGALRRGVHIIQHTPVIGLLRDGARVTGVQTEAGPISAG
                     IVLSAVGGRVTELAGMADVRLPIRTHPLQALVTNAYAQGLGPIVSDSELLCYVSQTAR
                     GQMLMGAEFDSQPSYSMASSFEFLQSVAAKVTRFLPFLRDLRVLRQWTGICDISPDYS
                     PIMGETGADGLLVTTGWGTWGFKAIPAGGEAMAEQIATGVAPSLIASFGLDRFARDHA
                     MADQGSAGTR"
     gene            4165..4401
                     /locus_tag="VGO32_03785"
     CDS             4165..4401
                     /locus_tag="VGO32_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEV7199903.1"
                     /translation="MLWITCPTCGERPVTEFRFGGELPHVPEHLTDDAERNLDYVWMF
                     DNVAGVTTERWFHATGCRRWLTLRRDTRTDQVVA"
     gene            4398..4805
                     /locus_tag="VGO32_03790"
     CDS             4398..4805
                     /locus_tag="VGO32_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7199904.1"
                     /translation="MTDALFPTPDMEITHILVVSDIAASERWYVDVLGAELVGSYGSS
                     VVLRFAGAWLLLVTGGEPTADKPMITFAAPSDPGRVDHSFTIRVQDCQASYEVLRARG
                     TEFLTPPYDWGGEVRCFFRDPDGHLFEISERRG"
     gene            complement(4813..5514)
                     /locus_tag="VGO32_03795"
     CDS             complement(4813..5514)
                     /locus_tag="VGO32_03795"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV7199905.1"
                     /translation="MARLRITRLGHGGILYQSPADKWVWVDRWIGAPTYVAPPEPWQV
                     DVVAPTHGHFDHVGDDAVDLVDLAGRGGTVVGSHELSLYLGERGVEAVGMNKGGTFEA
                     AGIGFTMVHADHTGAVSLTGGGPTSREVGCWGWVIRFEDGTTVYHTGDTDLFGDMALV
                     RERWSPTIAVMPIGGHYTMGPADAARAVALIGARTVIPVHYGTFPILAGTPTELAEAT
                     DAEVVALEPGETWEA"
     gene            complement(5519..5959)
                     /locus_tag="VGO32_03800"
     CDS             complement(5519..5959)
                     /locus_tag="VGO32_03800"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7199906.1"
                     /translation="MAAELLRREFSVEQPLPEAWRALAEVSAWPGWAPHLRRVQVEPA
                     GPIGPNSTGSLSFRPLGRSQFRVSSYAEGAGWEWVGKVLWLTIRYDHRFAQDGSRTRM
                     TWTVSEDGDRRSLLGRLFASVYGRLVDRAIPRLQEQLKSANRGA"
     gene            complement(5972..6670)
                     /locus_tag="VGO32_03805"
     CDS             complement(5972..6670)
                     /locus_tag="VGO32_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7199907.1"
                     /translation="MTDETVIQRTGVAAEPGHAIGIQVGAFGPWQANAYLVWDGQSTD
                     ALVLDPGMGAAAVLVARASEIGLRIHLVANSHGHIDHIFDNAAITRASGAPLAIHPDD
                     AYRLAGDNIYGFEVEPSQADRDLLEGETLSIGQLTFRILHTPGHSEGSVCLYEADRGL
                     LLSGDVLFRASYGRTDLSGGDDAQMVVSLTRLHHEIPPETRVLPGHGAETTMAREASW
                     LDRVATGGRLVIPG"
     gene            complement(6682..7446)
                     /gene="lipB"
                     /locus_tag="VGO32_03810"
     CDS             complement(6682..7446)
                     /gene="lipB"
                     /locus_tag="VGO32_03810"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEV7199908.1"
                     /translation="MAQMRDISLPPREPIAPAPSVRAPNTNPSRPLLLGDGGVLAVEW
                     LGRVPYRPTWELQNELAERRRRREIGDRVVLLEHDPVYTLGRGGDETHLLATPERLAA
                     IGAELVRIDRGGDITFHGPGQLVAYPIVELADPLDLRRYVRALESAVIATAFRFGVRA
                     DRVDGLPGVWVDGTRKLAAVGVRVRRGVTTHGLALNVSTDLAWFDEMVPCGIPGCQVT
                     SLERETGNVLALTEVADVLAADLARAFGLRLAPDPR"
     gene            complement(7450..7983)
                     /locus_tag="VGO32_03815"
     CDS             complement(7450..7983)
                     /locus_tag="VGO32_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7199909.1"
                     /translation="MRKHLEILAGRLAITHRQLLDAAEACTDDDLTWRPGPTSPPIAF
                     HLWHTARWADRWAETVGGAPQRWLREGLTASWGFAGALGAEDTGMLIPDDEASDLPFP
                     RRDQLTTYLRGAYNDLEQAVGALDDDSMLGLRDDLLGKEAPLADSLTRHLAHVNRHLG
                     MVEALRGLRGGHGTATV"
     gene            complement(7989..9035)
                     /gene="lipA"
                     /locus_tag="VGO32_03820"
     CDS             complement(7989..9035)
                     /gene="lipA"
                     /locus_tag="VGO32_03820"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEV7199910.1"
                     /translation="MPQDFSSLGELSLVEPDGGAGAGHHAPSEVMYPGGPDTDSGLPR
                     PGEPIPENAGPGGRRPEWLKVRIGAGETYAGLRRLMRDEALNTVCEEARCPNQGECWT
                     LGTATFMVLGSVCTRNCGFCAITTGRPPETDLDEPRRVAEAAKRMGLKHAVVTMVARD
                     DLPDGGAAVVADTVRQIRLASPGTSIEVLISDLNGREPDIRTVVESQPEILNHNVETV
                     PRLQRPVRRRARWDRSVEVLTTGRRLAAGIGHPMTTKTGLMVGLGETWDELVEAMGML
                     REAEIDVLTIGQYLRPSRSHLPLAKYYTPSEFMALRRIGLEMGFAHVQSGPLVRSSYH
                     AAEQIPGRDLAAVG"
     gene            9075..>9356
                     /locus_tag="VGO32_03825"
     CDS             9075..>9356
                     /locus_tag="VGO32_03825"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV7199911.1"
                     /translation="MPFNPTAAADGLEAGIRVMGTPARADAEKKYLKSDLIFLGATVG
                     QTRDTVKAYLRETTLDHASLEALVTTLWSAPIFERRMAAAMLLDLSPGLL"
BASE COUNT         1335 a         3272 c         3304 g         1445 t
ORIGIN      
        1 aggttttcca tgaccgtcag ctcgacgtcg aggttgtcct cctgcggcac gaccccgatg
       61 cgggacttga tccgcgtccc gtccttgctc gggttcatgc ccaccacgct cagcgcaccg
      121 tcggtcaccg ggctgatgca cgcgatcatg cgcatggtcg aggtcttgcc cgcgccattg
      181 ggcccgagga acccgaacgc ttccccgacg tggacatcga aatccacgcc gtcgaccgcc
      241 gtgaagtcgc cgaagcgctt cgtcagttcg cgggcatgga tcaggacgtc ggtctgggcg
      301 gtcatgggga ccgctgattg tagggaccga tgccgaaccc gtcaggcgcg tccggcgggt
      361 ccagtggagc agcgcggtgc tagcctcggc tcatgagccc ggtcgccggt cagttcctgc
      421 tggacgggtc cgcgctcggg ttcgagccgg gagacaccgt cgcggtcgcc gtcgtccgcg
      481 ccgcccagca cccgggccac ggcgggacgt tgtgcctggc aggtgactgc ggcaactgcg
      541 tcgccgaagt cgagggcatt gcctacgtcc gcacctgcct gaccccggcc gcggacggga
      601 tggccgtgac ccgccatccg accgacggtc ccccgcgctg gcacgccgag ccgcgcgaga
      661 cggaggtccc ggttgcgcat gacgagaccg atgtcgcagt catcggcggt ggcgatgccg
      721 ggcgggccgc cgcaacggcc gaggagcagg cgggtcgccg cgtactcgtc ctggacggcc
      781 ccgcaggcga cgaggtcgtg gccgtgtacg ccggcccaac ggtcatcgtc cgtcgccatg
      841 gggcgatggt ccacatccgc gcgacggagg tggtggtcgc caccggcacc gcggagaccc
      901 aacccgtggg gccaggcagt cggttgatcg gtctcctgac ctcccgtgcc gcccagcaac
      961 tccacgcggc gggggtggac ctgggtcggg cagtcgccgt cggtcctgtg ccggccggcg
     1021 tcccatgcac ggctcttcgc ggttcgctgc tgcggctgga gggggattcc cgggtcagcg
     1081 cggtggtcac tcgtgacgcg gaaggggaga accgggtcga gtgcgacacc gtgatcctgg
     1141 atctgggtcg gtcgccgcgc gacatgctgt cccggatggc gggcgacctg ccggtccggg
     1201 tcgtgggctc ggccgccgag acgcacccgc tcccgccgcc accgaccgcg ggcgtggtgt
     1261 gcccgtgcgt gggcaccacc gtcgacgacc tggagggcgt ctggtcgcgt ggcttccgtg
     1321 agctcgagct catcaagcgg gccagtctgg cgggaacggg cacgtgccag gggagcgtgt
     1381 gcatgcccca cgtcctgtcg tggatcgcgg ctcggaccgg ggaggcgccc ccgccgttca
     1441 ccgcgcgccc cgcatcgcgc cagatcaccc tgggcgaggc cgcggccgat gtacacctcg
     1501 acgccttccg tcggaccccg ctccatgacg agcacctcgc gctcggcgct cggatggatc
     1561 ggttcggtgg gtggtggcgg ccgtggcact acggcgacca cctggccgag taccgcgccg
     1621 tccgggaggc ggtgtcgctg ggcgacgtct ccaccctcgg caagttcatc gtcgccggac
     1681 aggacacggt cgagttcctc cagcgcctgt accccaccga tgtctcgacc attcgccccg
     1741 gccgctcccg ctacgcgctc ctgctgaacg agcgggggca cgtcatcgac gacgggatga
     1801 tctgccgcga gaccgaggac cggttcgtcc tgacgttcac taccggcggc gccggaaacg
     1861 ccgagatgtg gatgcgcgac tggctcgaga cctggggcct gcaggtccac cttatggacc
     1921 ggaccatgtc cctcgccgcc atcaacgtca ccggtccgcg agcggccgaa ctactggcac
     1981 gcgtcgggct aaccgatccg ctccgcttcc tccagcacgc ccatgtcgag gtcgccggga
     2041 tcccggccca cgtcatgcgt ctgagcttca ccggcgaggc ctcgttcgag atccaccatc
     2101 ccgttgaccg gtcggtcgac ctgtggcgcg gcctcatggc gatggggggt gacatgggca
     2161 tccggcccca cggcctccag gcactgttcg ccctgcgcct ggaaaagggc cacgtgatca
     2221 tcggcatgga caccgagctg gactccaccc cgcggcggct gggcatggac tgggcggtga
     2281 acatggacaa gcgcgacttc ctcggccgcg acgccctggc tcggaccgcg gccctgcccg
     2341 accaccggcg tctcgtcggc ctgacgatgg acggaccggc ccccaccgag ggcagcccga
     2401 tctggtccga tggcgacgtc atcggtcatg tcacctcgac ctttgcctcg cccacccttg
     2461 gtcacgcggt gatgctgggc tggcagaagc ggaccccatt ccccgaacag gtagaggtgg
     2521 atgggcggac tgccacggtg ggcgccccgc cgttctacga cccggagggt gcccgtgcgc
     2581 gcgcctgagc cgctgcccgc cacacgagtc gtggccgcac cagccgtgct ggacgggatc
     2641 gcgaccgacg gtggaatcac cctccgcctg gcggtcgacg aggccctggt cctcggaggg
     2701 ggagccgtgg ttgacgacga ggccgccctg gtggaggacg aggccgggtt cgtcggctgg
     2761 tggctgacgc ctgacgagct gacggaggtc gcccatcacc tcgaatggac gattccggct
     2821 gaacgaccgg cgcttgccca gggcctggtg gccggcgtcc cggcccggct gtggctgacc
     2881 gaggagcggt cgctcctcct ggtctcgggc gcgtacgccc acgagctcgt cgagcgtctg
     2941 ccatgaacga gttcgtcgag accatcctcg acctgccctg gcacgagccg aagggttcgt
     3001 acgacgtcgt catcgtgggc ggcggtgggc acggcctcgc gacggcgtat tacctcgcca
     3061 cgcgacacgg catcacgaac gtagcggtgg tcgaggccaa ctacatcggc tccggcaaca
     3121 gcggccggaa tacgaccatc atccgggcca actacgggat ccccgagtcc gtgcgcttct
     3181 accagcacag cctcgagctg tacgcgactc tcgaggacga gaccgagtgc tggatcatgc
     3241 attcgaccaa gggcctgctg tggctcgccc acaccgagtg ggggatgcgc gccgagcggg
     3301 cgcggacgct catgaaccag gcctgcggcg ccgaaacggt gatggtcacg ccggaggagg
     3361 cgaaagccct gtgtccggcg ctggacctgt ccggcggcgg tcgctacccg atccttggcg
     3421 cctcgtacca tcccggcgcg gctaccgccc gccacgaccg agtggtctgg gcctatgccc
     3481 agggcgcgct ccggcgcggg gttcacatca tccagcacac cccggtcatc ggcctgctcc
     3541 gtgatggcgc cagggtgact ggcgtccaga ccgaggccgg accgatctcg gccgggatcg
     3601 tcctcagcgc ggtcggcggc cgggtgaccg agctggccgg catggccgat gttcgactgc
     3661 cgatccggac ccatccgctc caggcgttgg tcacgaacgc gtatgcccaa ggtctgggcc
     3721 cgatcgtcag cgactcggag ctgctgtgct acgtctcgca gacggcgcgc ggccagatgc
     3781 tgatgggcgc ggagttcgac tcccagccga gctactcgat ggcctcgtcg ttcgagttcc
     3841 tccagagcgt ggccgccaag gtgacccggt tcctcccgtt ccttcgcgac ctccgggtcc
     3901 tccgccagtg gaccgggatc tgcgacatct cgccggacta ctcgccgatc atgggcgaga
     3961 ccggcgcgga cggcctgctg gtgacgaccg gttgggggac ctggggcttc aaggccatcc
     4021 cggccggcgg cgaggcgatg gccgagcaga tcgccaccgg cgtggcgccg tcgctgatcg
     4081 catccttcgg gctggaccga ttcgcccgcg accacgccat ggccgaccag ggatcggccg
     4141 ggacccggtg aggagtctcg tctgatgctg tggatcacgt gcccgacctg cggggagcgg
     4201 ccggtgaccg agttccggtt cggcggcgag ctgccccacg tgccggagca cctgaccgat
     4261 gacgccgagc ggaatctgga ctacgtctgg atgttcgaca acgtcgccgg cgtcaccacc
     4321 gagcgttggt tccatgccac aggctgtcgc cgctggctga ccctccgtcg tgacacccgc
     4381 accgaccagg tggtggcttg accgacgccc tgttcccgac tccggacatg gagatcacgc
     4441 acatcctggt cgtgtccgac atcgcggcct cggagcggtg gtacgtggac gtgctgggcg
     4501 ccgagctggt cggctcgtac gggtcctcgg tcgtgctccg cttcgccggg gcgtggctcc
     4561 tgctcgtgac cggcggggag ccgaccgcgg acaagcccat gatcaccttc gccgccccct
     4621 ccgacccggg ccgcgtggat cactcgttca cgatccgggt ccaggactgc caggcctcgt
     4681 acgaggtgct ccgggcccgc ggtacggagt tcctgacccc gccctacgac tggggtggcg
     4741 aggtccgatg cttcttccgc gaccccgatg gccacctgtt cgagatcagc gagcgccgcg
     4801 gctaaggctc gtctaggcct cccatgtctc gcccggctcg agggccacga cctcggcatc
     4861 agtggcctcg gccagctcgg ttggcgtgcc ggcgaggatc gggaaggtgc cgtaatggac
     4921 cgggatgacg gtccgcgccc cgatgagggc gactgcgcgc gccgcatcgg ccggacccat
     4981 cgtgtaatgg ccgccgatgg gcatgaccgc gatcgtgggc gaccagcgct cacggacgag
     5041 ggccatgtcg ccgaacaggt cggtgtcgcc ggtgtgatag acggtcgttc cgtcctcgaa
     5101 gcggatgacc catccccagc agcccacctc gcgcgacgtg ggaccgccgc cggtcaggct
     5161 cacggccccg gtgtggtcgg cgtggaccat cgtgaagccg attcccgcgg cctcgaacgt
     5221 gccgcccttg ttcatgccga ccgcctcgac acccctctcc ccgagataga ggctcagctc
     5281 gtgcgacccg accaccgtcc cgccgcgacc agccaggtcc acgaggtcga ccgcgtcatc
     5341 gccgacgtgg tcgaagtgac catgggtcgg ggcgacgacg tccacctgcc acggctccgg
     5401 cggcgcgacg taggtcggcg cgccgatcca tcggtccacc cagacccact tgtcggcggg
     5461 cgactggtac aggatgccgc cgtggccgag ccgggtgatg cgcagtctgg ccatgtggtc
     5521 atgctcctcg attggcggat ttgagctgct cctgcaggcg agggatggct cgatccacca
     5581 atcgcccgta gaccgatgca aagagccgcc cgagtaggga gcggcggtct ccgtcctcgg
     5641 agacggtcca ggtcatccga gttcggctcc cgtcctgggc gaagcggtgg tcgtagcgga
     5701 tcgtgagcca aagcaccttg ccgacccact cccagccggc tccctcggcg taggaggaga
     5761 cgcggaactg gcttcggcca agcggtcgga atgagaggct gcccgtgctg ttcgggccga
     5821 tcgggccggc cggctcgacc tggaccctcc ggaggtgggg agcccagccc ggccatgccg
     5881 acacctcggc gagtgcgcgc cacgcctcgg ggagcggctg ctcgaccgag aactcgcggc
     5941 gcaggagttc ggcggccacg agccgagtct atcaaccggg gatcacgagc cgtccgccgg
     6001 tcgccacgcg gtcgagccat gatgcctcgc gggccatggt cgtctccgcg ccgtgcccgg
     6061 gcaggacacg agtctcaggt gggatctcgt ggtgcagccg ggtgagcgag acgaccatct
     6121 gggcgtcatc gccaccggac aggtccgtgc ggccgtacga ggcgcggaac aggacgtcgc
     6181 cgctgagtag cagcccgcga tcggcctcgt acaggcagac cgatccctcc gaatggccgg
     6241 gcgtgtgcag gatccggaac gtcagctggc cgatggacag ggtctcgcct tcgagcaggt
     6301 cccgatcggc ttggctgggc tcgacctcga agccgtagat attgtccccg gcgagccgat
     6361 acgcgtcgtc agggtggatg gccagcgggg ctccgctggc ccgggttatg gccgcgttgt
     6421 cgaagatgtg gtcgatgtgg ccgtggctgt tggccacgag atggatccgg aggccgatct
     6481 cggaggcacg cgcgacgagg acagctgccg cgcccatgcc ggggtcgagt accaacgcat
     6541 cggtcgattg tccgtcccac accaggtacg cgttcgcctg ccacggaccg aacgctccga
     6601 cctggatccc gatagcgtga ccgggttcgg ccgccacccc ggttcgctgg atgacggtct
     6661 cgtcggtcac cggctggcgg ctcagcgcgg atccggggcg agccgaaggc cgaacgcgcg
     6721 ggccaggtcc gcggccagga catcggcgac ctcggtcaat gccagcacgt tgcccgtctc
     6781 ccgttccagg ctcgttacct ggcaaccggg gatgccgcac gggaccatct cgtcgaacca
     6841 ggccaggtcg gtgctgacat tgagcgcgag gccgtgggtc gtcaccccgc gcctgacccg
     6901 caccccgacc gcggcgagct tgcgggtgcc gtccacccac acgccgggca ggccgtccac
     6961 ccggtccgcc cggactccga accggaaggc ggtcgcgatg accgccgatt ccagggcgcg
     7021 gacgtagcgt cggaggtcga gcgggtcagc gagctcgacg atggggtagg cgaccagctg
     7081 gcccgggcca tggaacgtga tgtcgcctcc gcgatcgatc cggaccaact cggccccgat
     7141 ggccgccaac cgctccggcg tggccagtag atgtgtctcg tccccgccgc ggcccagggt
     7201 gtagaccggg tcgtgctcga ggagcaccac gcggtcgccg atctcgcgcc gccgccgccg
     7261 ttcggcaagc tcgttctgga gctcccaggt gggccgatac gggacgcggc cgagccattc
     7321 cacggccagt acgccgccgt ccccgagcag gagaggacgc gacgggttcg tgttgggcgc
     7381 ccggaccgaa ggcgccggcg cgatcggttc gcgaggtggg aggctgatgt cgcgcatctg
     7441 cgccatgggt cagaccgtcg ccgtgccgtg gcccccgcgg agtccccgca gggcctcgac
     7501 catccccagg tggcgattga cgtgggcgag gtgccgcgtc agggagtcgg ccaacggcgc
     7561 ctccttgccg aggaggtcat cgcgaagccc cagcattgag tcgtcgtcga gagctccaac
     7621 cgcctgctcg aggtcgttgt aggcgcctcg caggtacgtg gtcagctggt cacgtcgcgg
     7681 aaatgggagg tcgctggcct cgtcgtccgg gatcagcatg ccggtgtcct ccgcgcccag
     7741 cgccccggcg aagccccatg aggcggtcag tccctcacga agccaccgtt gcggggcgcc
     7801 gccgaccgtc tccgcccagc ggtcggccca tcgcgcggta tgccacagat gaaaggcgat
     7861 cggaggggag gtgggccccg ggcgccaggt caggtcgtcg tcggtgcagg cttcggccgc
     7921 gtcgaggagc tggcggtgcg tgatggccag ccgcccggca aggatctcga ggtgcttccg
     7981 catcggggtt agccgacggc cgccaggtcg cggccgggga tctgctcggc ggcgtggtag
     8041 ctcgagcgca cgagcgggcc ggactggacg tgggcgaagc ccatttcgag cccgatccgg
     8101 cgcagggcca tgaactcgga cggcgtgtag tacttggcca gcggcaggtg gctccggctc
     8161 gggcgcaggt attgcccgat ggtcagtacg tcgatctccg cttcgcgaag catgcccatg
     8221 gcctcaacca gctcgtccca ggtctcaccc agcccgacca tcagcccggt cttggtggtc
     8281 atcggatgac cgatgccggc cgccagccgg cggccggtgg tgaggacctc gacgctgcgg
     8341 tcccaccgtg cccggcggcg gacggggcgc tggaggcggg gcacggtctc gacgttgtgg
     8401 ttcaggatct cgggctgcga ctcgacgacg gtccgaatat ccggctcgcg accgttcagg
     8461 tcgctgatga ggacctcgat gctcgttcct gggctcgcga gccggatctg gcgcacggtg
     8521 tcggccacca ccgcggcgcc gccgtcgggc agatcgtctc gggccaccat ggtcaccacc
     8581 gcgtgcttca gtcccatccg cttcgccgcc tctgcgaccc ggcgtggctc gtcgaggtcg
     8641 gtctccggcg gacgaccggt cgtgatcgcg cagaacccgc agttccgggt gcacacgctg
     8701 cccaggacca tgaaggtcgc ggtgcccagg gtccagcact ccccctggtt cgggcaccgg
     8761 gcttcctcgc acaccgtgtt gagcgcttcg tcacgcatca gccggcgcag gccggcatag
     8821 gtctcgcccg cgccgatccg gaccttgagc cactccggcc gtcggccgcc tggccccgcg
     8881 ttctcgggga tcggctcgcc gggccggggc aggccactgt cggtgtccgg cccgccgggg
     8941 tacatcacct ccgacggagc gtggtgtccg gcgccagccc cgccgtccgg ttcgaccagg
     9001 gacagctcac ccagggacga gaaatcttgc ggcatcgggg agagtctagc gaaggccgcg
     9061 actagactcg gatcatgccc ttcaacccga cggctgcggc ggacgggctc gaggctggga
     9121 tccgtgtcat gggcacaccg gcccgggcgg atgccgagaa gaagtacctc aagagcgacc
     9181 tcatcttcct gggcgcgacg gtcgggcaga cccgggacac ggtgaaggcg tacctcaggg
     9241 agacgaccct cgatcacgcc tcgctggagg cactggtcac gaccctgtgg agcgcaccga
     9301 tcttcgagcg gagaatggcg gcggccatgc tcctggacct cagtcccggg ctcctc
//
LOCUS       DASYGU010000142         5932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1823918, whole genome shotgun sequence.
ACCESSION   DASYGU010000142 DASYGU010000000
VERSION     DASYGU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823918"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VGO32_03830"
     CDS             complement(<1..634)
                     /locus_tag="VGO32_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV7199912.1"
                     /translation="MRATAAARPLVGGPSPGRATLAIGGGVVLVLVASLTAVAVGSAD
                     VGLGEAIGIIAQRMGFPVSETWASTAPTILFEVRLPRVLAGLLVGAGLGCAGVVFQAL
                     LRNPMADPYIIGSAAGASLGAVLAITAPVLVPALAVGAGTAWLGIGLAQGMAFAGGLG
                     AVALVLAVARTGGRTNMVTLLLTGYAVSSLLAAGVAVLLVSSGRALASVVG"
     gene            complement(642..1616)
                     /locus_tag="VGO32_03835"
     CDS             complement(642..1616)
                     /locus_tag="VGO32_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEV7199913.1"
                     /translation="MAPRRIRILVPLLLVLAACQPSASSSAPPSAEASLVPSASPPTA
                     VIYPQTLTDDAGREVAIPAEPARIVSLAPSNTEIVCALGACDRLVGVTDFDDYPAEVD
                     DVDDVVIGQVVDIELVVAADPDLILAAGNELTPSAVITELADLDYPVLVLYPESLHEV
                     THNIELVGQAINTVAEADDVVAEMQTRIEAVEEAVAGEPTPRTFYEVGLFEGAIYTAA
                     EDSFLAGLIATAGGDAILGDPLTTAIALEDLVAANPELILLGDGAYDVSITPESVAAR
                     PGGWAEMTAVVNGDVLVMAEDPVITRPGPRIVDGLEALALAIHPDAFD"
     regulatory      complement(1644..1784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1796..2077)
                     /locus_tag="VGO32_03840"
     CDS             complement(1796..2077)
                     /locus_tag="VGO32_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199914.1"
                     /translation="MHSGMIGKVEKAMRYAHEPDRVTIDRLEATFAGDNGSHKVSLDG
                     EHWHCDCHLFEQAGGCTHTLAIQKMLDPMLSDTARETPLYGHVEVGAGV"
     gene            complement(2125..3297)
                     /locus_tag="VGO32_03845"
     CDS             complement(2125..3297)
                     /locus_tag="VGO32_03845"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEV7199915.1"
                     /translation="MTDARDFFGYKDPDEVPGRQDESDDGPDPDAQPTFDEAITLDAP
                     AVIARIDREDMLGRVRGLPRQLAGARQAAAGLALTDRHRDVDLVVVLGMGGSAIGADL
                     VAAAAGDRLRVPLLVQRDYHLPTGLGERTLVVAASHSGDTIETITAAAEARERNLPLV
                     VIAAGGTLGRLAEADGTPWLRYEQPGQPRAALGWSVGLLHELLVAAGLLTDPDPLGPT
                     VALLDEIMERLGPGVDTDANPAKQLAWSILGRVPVVYGAGIMAPVARRWKTQFNENAK
                     SWATYEPMPEANHNAIEGSLNPRELADAPFVIEVRDPTESDAIAARYRVVEELLGERA
                     TNRATIWAEGPSPLARVMSAVAWGDLVSVYLAILYQTDPTPVTLLAMLKERLARSD"
     gene            complement(3305..4762)
                     /locus_tag="VGO32_03850"
     CDS             complement(3305..4762)
                     /locus_tag="VGO32_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEV7199916.1"
                     /translation="MTTIRFGTDGWRAAIAEDFTFHNVRRCARGVADYLAEQGTIDRG
                     VVVAHDRRFASEHFARACVEVLTAHGIRCHTPPGSVPTQVVSFFTRELHAGAGIVITA
                     SHNPWTDNGFKVKADTGGAAAPAMLAAIEATMDRYPEDEVPPRREYEDAVAAGLVEVF
                     DPYPRFREQLAAIVDLERIRGASGKVLVEPMWGSGAGWFTRLLDGGTLTIRELHTERN
                     PFFGGVNPEPIRPNIDEWLEEIPRWGADIGIAFDGDADRVGMATEEGIFVNQLQVYGL
                     LYWYLLEVRGRIGPAVYTVTTTSMARRLAALFGTEAYETGVGFKFVGPKMEETHAEIG
                     GEESGGFGFGIHIPERDGLAAGLFLLDLWLSRERRASEVLAELQALAGPSYYNRIDVR
                     FSREEYPAIKRDTLARLEHEAPTTLGGREVVRRQALDTGDGFKFHRDDESWLLIRFSG
                     TEALLRIYVEAGSPEDVETLLAEGRRIATAAASAA"
     gene            complement(4771..5358)
                     /locus_tag="VGO32_03855"
     CDS             complement(4771..5358)
                     /locus_tag="VGO32_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199917.1"
                     /translation="MARTARIGPVTRETVVLAFWHEYNLALYAIASARRGDLGHASFT
                     TDTDRGTVIQTIFESLARRPGQVVILRLPDEGQAAQSRALARRLGELGASGYSVVVTP
                     DGPWGPYRVAKPGVLIVARQSGLPVVPVAVRARPAIRLARRWDRQLLPLPFSRLTLVT
                     GSPMTISRDQPLRPLVPAVQAALDAVTATAEGRLP"
     gene            complement(5402..>5932)
                     /locus_tag="VGO32_03860"
     CDS             complement(5402..>5932)
                     /locus_tag="VGO32_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEV7199918.1"
                     /translation="KALVASGALWNAGVFVWTGYAFRQAMERFQPALTEALDQIGRIP
                     RTPGWQTDARALLEPLPAISLDAGIAEPAAADGRMAVVALDAGWSDIGSWSALLEALS
                     RQSGKSLVASGQHLDRGSQRVLVHGGERLVVTVGLKDIIVVDTPDALLICARDRAEEI
                     KPILDEIGEATGDRYL"
BASE COUNT          903 a         2085 c         2122 g          822 t
ORIGIN      
        1 agccgacaac cgaggcaagc gcccgaccag agctgacgag caggacggcg accccggcgg
       61 ccaggagcga ggacactgca tacccggtca gaagcagggt gaccatgttc gtccggccgc
      121 ccgtgcgggc gaccgccagg acgagcgcca ccgcgccgag accgccggcg aacgccatgc
      181 cctgggccag cccgatcccg agccacgccg tgcccgcccc aacggcgagg gctggcacga
      241 gcacgggggc ggtgatggcc agcacagcgc cgagggaggc gcccgccgcg ctgccgatga
      301 tgtacggatc ggccatcggg ttgcggagca gggcctggaa cacgacgcca gcgcagccca
      361 gccccgcgcc cacgagcagg ccagccagca cccgcggcag acggacctcg aacaggatcg
      421 tcggcgcggt cgacgcccat gtctcgctga ccgggaaccc catccgctgg gcgatgatcc
      481 cgatcgcctc gccgagcccg acatcggcgc tgccgaccgc gaccgcggtc agcgacgcga
      541 cgagcacgag gaccacccca ccgccgatgg cgagcgtggc ccgcccgggc gagggcccac
      601 cgacgagcgg tcgggcagca gcggtggccc gcatcaggcg ctcagtcgaa ggcgtcggga
      661 tggatggcga gggcgagggc ctcgagccca tccacgatcc ggggtccggg ccgcgtgatc
      721 accgggtcct ccgccatgac cagcacgtca ccgttcacca ccgcggtcat ctccgcccag
      781 ccaccgggcc tcgcggcgac cgactccggg gtgatcgaca cgtcatatgc cccatcccct
      841 agaaggatga gctccggatt tgcggccacc aggtcctcga gcgcgatggc ggtggtcagc
      901 ggatcgccga ggatcgcatc cccgccggct gtcgcgatca gcccggccag gaaggagtcc
      961 tcggcggcgg tgtagatcgc cccctcgaac aggccgacct cgtagaaggt ccgcggcgtc
     1021 ggctcgccgg cgaccgcctc ctcgaccgcc tcgatccggg tctgcatttc ggcgaccacg
     1081 tcatcggctt cggcgacggt gttgatcgcc tggcccacga gctcgatgtt gtgggtcacc
     1141 tcgtggagcg actcggggta caggacgagg accgggtagt cgaggtcggc cagctcggtg
     1201 atgaccgcgc tgggcgtcag ctcgttgccg gcggccagga tgaggtccgg atcggcagcc
     1261 acgacgagct cgatgtcgac tacctggcca atgaccacgt cgtcgacgtc gtccacctcg
     1321 gccggatagt catcgaagtc ggtcacgccg accaggcggt cgcacgcgcc gagcgcgcag
     1381 acgatttcgg tgttcgacgg ggcgaggctg acgatgcggg ccggctcggc ggggatcgcc
     1441 acctcgcggc cggcgtcatc ggtcagggtc tgggggtaga tgaccgcggt cggcggcgat
     1501 gcgctgggca cgaggctggc ttcggcgctc ggcggtgcgc tcgagctcgc ggacggctgg
     1561 caggcagcca gaaccaggag gaggggaacc agaatacgga tgcggcgcgg cgccatggtg
     1621 atgtcactcc ctttctcacg agggttgaga catcgcgttg tggctgggcg acctggctcc
     1681 cggagcgact ccggtcacag ttgcgggaca gcaccggtct tgcaccggct tcgccattta
     1741 cccgccggcc tttcggcggc ggcaccccac gcggtcttcg attggtcggc cgatgctaca
     1801 ccccggcgcc gacctcaacg tggccgtaga gcggggtctc gcgggcggtg tcggacagca
     1861 tggggtcgag catcttctgg atggcgaggg tatgggtgca gccgccggcc tgctcgaaca
     1921 gatggcagtc acagtgccag tgctcaccat cgagcgaaac cttgtgggac ccgttgtcgc
     1981 cggcgaaggt ggcctcgagc cggtcgatcg tcacccgatc gggctcgtgg gcgtagcgca
     2041 tcgccttctc gaccttgccg atcatgcccg aatgcacgat tgctactcct ccaggcgccc
     2101 gagggcgccc tcttcaggcg cccgtcagtc tgacctggcg agccgctctt tcagcattgc
     2161 cagcagtgta accggggtgg ggtcagtctg gtagaggatg gccaggtaca cgctcacgag
     2221 gtcgccccag gccacagccg acatgacccg cgccagcggc gacggcccct cggcccagat
     2281 cgtggcccgg ttggtggccc gctccccgag gagctcctcg accacgcggt agcgggccgc
     2341 gatcgcatca ctctcggtcg ggtcgcgcac ctcgatcacg aatggcgcgt cggccagctc
     2401 gcgcgggttc agcgaaccct cgatcgcgtt gtggttggcc tccggcatgg gctcgtaggt
     2461 cgcccatgac ttcgcgttct cgttgaactg ggtcttccag cgtcgcgcga ccggcgccat
     2521 gatcccggcc ccgtagacca ccggcacccg gcccaggatg ctccacgcca gctgcttggc
     2581 ggggttggcg tcggtgtcca cgcccggccc aagccgctcc atgatctcgt ccaggagggc
     2641 cacggtcggg cccaacgggt ctggatcggt cagcaggccg gccgcgacga ggagctcgtg
     2701 gaggagcccc accgaccagc ccagggccgc gcgcggctgg ccgggctgct cgtagcggag
     2761 ccacggggtg ccgtcggcct cggccaggcg gccgagggtg cctccggcag cgatcaccac
     2821 caggggcaga ttgcgctctc gcgcctcggc cgccgcagtg atggtctcga tcgtgtcccc
     2881 cgaatgcgag gcagcaacca ccagggtccg ctcgcccaga ccggtgggca ggtggtagtc
     2941 gcgctggacg aggagcggca cgcggaggcg gtcaccggcg gcggcggcga ccaggtcggc
     3001 gccgatggcc gatccaccca tgcccagcac gaccacgagg tccacgtccc ggtgacgatc
     3061 ggtcagggcc agcccggccg ccgcctgccg ggcaccggcc agctgacgcg ggaggccgcg
     3121 aacccgtccc agcatgtcct cccggtcgat gcgcgcgatg accgccggcg cgtcgagtgt
     3181 gatcgcctcg tcgaaggtgg gctgtgcgtc cggatccggg ccgtcgtcgg actcgtcctg
     3241 ccggccgggg acctcgtccg ggtccttgta gccgaagaag tcgcgggcgt cggtcaccgg
     3301 gcgctcacgc ggccgacgcg gcggcggtcg cgatccggcg gccttcggcc agcagggtct
     3361 cgacgtcttc cggcgagccc gcctcgacgt agatccggag cagggcctcg gttccgctga
     3421 agcggatgag gagccacgac tcgtcgtcgc gatggaactt gaacccgtcg ccggtgtcca
     3481 gcgcctggcg ccggacgacc tcgcgcccgc cgagcgtggt cggagcctcg tgctccagac
     3541 gggcgagggt gtcgcgtttg atggccggat actcctcccg cgagaagcgg acatcgatcc
     3601 ggttgtagta cgacggaccg gccagggcct ggagctcagc cagcacctcg gaggcgcggc
     3661 gctcccggga gagccacagg tcgagaagga acaggccggc cgccagcccg tcacgttcgg
     3721 ggatgtggat cccgaacccg aatccgccgg actcctcgcc gccgatctcg gcgtgcgtct
     3781 cttccatctt gggcccgacg aacttgaagc cgaccccggt ctcgtaggcc tcggtgccga
     3841 acagcgccgc gagccgacgg gccatggagg tggtggtcac cgtgtacacc gccggaccga
     3901 tccgtccgcg cacctcgagc aggtaccagt acagcagccc gtagacctgg agctggttga
     3961 cgaagatccc ctcctcggtc gccatcccca cccggtcggc gtcaccgtcg aacgcaatcc
     4021 cgatgtcggc gccccagcgc gggatctcct cgagccactc gtcgatgttg gggcggatcg
     4081 gctccgggtt gaccccgccg aagaacgggt tccgctcggt gtggagctcg cgaatggtca
     4141 gcgtgccgcc gtcgagcagg cgggtgaacc agccggcgcc agacccccac atcggctcga
     4201 ccaggacctt gcccgacgcg ccgcgaatcc gctcgaggtc cacgattgcg gccagctgtt
     4261 cgcggaagcg cgggtacgga tcgaagacct ccaccaggcc ggcagccact gcgtcctcgt
     4321 attcgcgccg gggagggacc tcgtcctccg ggtagcggtc catagtggcc tcgatggccg
     4381 ccagcatggc cggggccgcg gcgccgccgg tgtcggcctt gaccttgaac ccgttgtctg
     4441 tccacgggtt gtggctggcg gtgatcacga tcccggcgcc cgcatggagc tcgcgggtga
     4501 agaacgacac gacctgggtc ggcaccgacc cgggcggggt gtggcaccgg atgccgtggg
     4561 cggtgaggac ctcgacgcag gcccgcgcga agtgctccga ggcaaagcgt cggtcgtggg
     4621 cgacaacgac tccgcgatcg atggttccct gctccgcgag gtagtcggca acgccgcggg
     4681 cacagcggcg gacgttgtgg aaggtgaagt cctcggcgat ggccgcacgc cagccgtcgg
     4741 tcccgaagcg gatggtggtc acgggcaggg ctatggtagc cgcccctcgg cggtggccgt
     4801 caccgcatcg agggcggcct ggaccgccgg cacgaggggc cgcagcggct gatcgcgcga
     4861 aatcgtcatc ggagaacccg tgacgagcgt caatcgcgag aagggcaggg gaagcagctg
     4921 gcgatcccag cgccgggcga gccggatggc gggtcgggcg cgaacggcga ccggaacgac
     4981 cggcaggccc gactgccggg ccacgatcag gacacccggc ttcgccaccc ggtacggacc
     5041 ccacggtccg tccggggtga ccaccaccga gtaccccgat gcgccgagct ctccgagccg
     5101 gcgggcgagg gcacgagatt gcgcggcctg gccctcgtcg gggagacgga ggatcaccac
     5161 ctgccctggg cgccgggcga gagattcgaa gatggtctgg atcaccgttc cccgatcggt
     5221 atcggtcgtg aacgacgcgt gaccgaggtc gccccggcgg gccgaggcaa tcgcgtacag
     5281 ggcgaggttg tactcgtgcc agaaggcgag cacgaccgtt tcgcgggtga ccggtcctat
     5341 gcgcgccgta cgcgccacga accgcagata ccacgccagg agccggccgc acaggcgggc
     5401 cctagaggta ccggtcgccg gtggcctccc cgatctcgtc caggatgggc ttgatctcct
     5461 ccgcccggtc gcgggcgcag atgagcaggg catcgggcgt gtcgaccacg atgatgtcct
     5521 tcaggccaac ggtgaccacc agccgctccc cgccgtggac gaggacccgc tgcgacccgc
     5581 ggtccaggtg ctggccggag gcaaccaggc tcttgccact ctggcggctg agggcctcca
     5641 gcagggccga ccacgaaccg atgtccgacc agcccgcgtc cagggccacg accgccatcc
     5701 gcccgtcggc ggccgccggc tcggcgattc cggcatcgag gctgattgcg ggcagcggct
     5761 cgaggagcgc acgggcatcg gtctgccagc ccggcgtgcg tgggatccgg ccgatctggt
     5821 ccagcgcctc ggtcagggcc ggctggaacc gctccatcgc ctggcggaat gcatacccgg
     5881 tccatacgaa cacgcccgcg ttccacagcg ccccggaggc gacgagcgcc tt
//
LOCUS       DASYGU010000143         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_897824, whole genome shotgun sequence.
ACCESSION   DASYGU010000143 DASYGU010000000
VERSION     DASYGU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897824"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="VGO32_03865"
     CDS             <1..1997
                     /locus_tag="VGO32_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199919.1"
                     /translation="FIEELLRSWISSGALAAEGGDRGGWRLAVAPESVPLPPTVQAIY
                     AAQLDDLPAPARLVARRASVAGRRFAVRALEPLEVPDPAEGLDRLRQRAFLRGPEADP
                     AGDQIYAYRHVLLRDAGYASLSRAERGRLHLALAIWLEQTAGIDVARVAEPIASHYEA
                     ALAALPGVGADPDERLRLTDAARSWLERAADAALAVAGHDAAIGLLGRAADLAAGDDR
                     EAARLRLRRGELMASSGNISAGVDEIQAALECFRTAYRADPSDMVARAGYGNAAAAMG
                     DAMIEQIRAIEARDLVAETLAEIGQPEDALVGRLLALHAWAKVFQGDRETTVAEVERA
                     LELVRGGDPRVEIQVRGWRASVRTEAQISVVEEWRELEVMARAQGMWAVAVRALLMRF
                     GNDPAASARDTLAAVRSGFDLATNHGLTEVASGSSSLIVGTQLGLGEWDEALALGFEN
                     VALAERNAYSRVLFRIWMALAPMLEARGDRDQLARYATWFDTVRSMFPEPPSPYAQVH
                     NAAIDRMMATVGLPPVNGRPMTVEILATEAYNNVEYVAALEITIRDWLAEGASDRAAE
                     ALERIELSYRDDDDFPPTLLIEASFALMHAWVLRAHGSGAVEAARTSADWSRRAEAPW
                     WLARAIRALPDGEATAEELAEAAEIEGRLRVREGASAPPV"
     gene            complement(2001..>2652)
                     /locus_tag="VGO32_03870"
     CDS             complement(2001..>2652)
                     /locus_tag="VGO32_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199920.1"
                     /translation="ATGGAVSLVLDAPFSRVGTDRRAFLQSYGLPERERDMTAQAIID
                     VRRAYDILVERDDVDPARLGYVGHSWGASTGSTLAAVDERPAALILIAPRPSWTGFLA
                     TSDDGWVAGARALVGAEKFDVYLEVMAPFDAMAIADQLDGERIYLQYGTIDEVVPPDV
                     AQELIEAVPDATLSTYEAEHALDDAATADRAAWLVERLGLGPISPETLATVGLPDE"
BASE COUNT          376 a          916 c          979 g          381 t
ORIGIN      
        1 tcttcatcga ggagctgctg cggagctgga tcagcagcgg ggcgctcgcg gccgagggag
       61 gtgaccgtgg cggttggcga ctggcggtag cgccggagtc ggttccccta cctccaaccg
      121 tgcaggccat ctatgcagcc caactggacg acctgccggc acccgcccgc ctggtcgccc
      181 gtcgggcctc ggtggcgggt cgtcgcttcg cagttcgcgc cctcgagcct ctcgaggtgc
      241 ctgaccccgc cgagggactg gaccgcctca gacaacgggc attcctgcgc ggccccgagg
      301 cagacccggc gggcgaccag atctacgcct atcgccatgt cctcctccgc gacgccggct
      361 acgcgagtct ttcccgcgcc gagcgcggcc gtctgcacct ggcgctggcg atctggctgg
      421 aacagaccgc cgggatcgat gtcgcccggg tcgccgagcc gatcgcctcc cattatgagg
      481 cggctctcgc ggccctcccc ggtgtaggcg cggatccgga cgaacgcctc cgcctgaccg
      541 atgccgcccg ctcctggctg gagcgggcgg ctgatgccgc cctggccgtc gcggggcacg
      601 acgcggcgat cggcttgctc gggcgagccg cggacctggc cgccggcgac gaccgcgagg
      661 cggcacgtct ccgcctgcgg cgcggtgagc tgatggcctc gtcggggaac atttctgccg
      721 gcgtggacga gatccaggca gcccttgagt gcttccgtac cgcctaccgc gcggacccgt
      781 ctgacatggt cgcgcgggcc ggttacggca acgccgcggc cgcgatgggg gacgcgatga
      841 tcgagcagat ccgcgccatc gaggcccgcg acctcgtggc cgagacgttg gccgagatcg
      901 ggcagccgga ggacgccctg gtcggccgcc tgctggctct ccacgcctgg gccaaggtct
      961 tccagggaga ccgtgagacg acggtcgcgg aggtggagcg agccctggaa ctggtgcgcg
     1021 gcggcgaccc gcgtgtggag atccaggtcc gcggatggcg agcctcggtg cgaaccgagg
     1081 cgcagatcag cgtcgtcgag gaatggcggg agctcgaggt gatggccagg gcccagggca
     1141 tgtgggcggt cgcagtccgg gccctgttga tgcggttcgg caacgacccg gcggcatcgg
     1201 cccgagacac cctggccgct gtccggtcgg gattcgatct ggcgacgaac cacggcctga
     1261 cggaggtcgc ctccggcagc agctccttga tcgtgggcac ccagctcggc ctgggcgagt
     1321 gggacgaagc cctggccctg ggattcgaga acgtcgcctt ggccgagcgg aacgcctaca
     1381 gccgggtcct cttccgcatc tggatggcac tggccccgat gctcgaagcc cgcggagatc
     1441 gcgatcagct ggcccgctat gccacgtggt tcgacacggt gcgttcgatg ttcccggaac
     1501 ctccgtctcc gtatgcgcag gtccacaacg cggctatcga ccgaatgatg gcgacggttg
     1561 gcttgccgcc ggtgaacggg cgaccgatga ccgtcgagat cctcgcaacc gaggcctaca
     1621 acaacgtcga gtacgtcgcg gcgttggaga tcacgatccg tgactggctg gccgaggggg
     1681 catcggaccg cgcggccgaa gcgctcgagc ggatcgagtt gtcgtaccga gacgacgacg
     1741 acttcccgcc gaccctgctc atcgaggcgt cattcgcgct gatgcacgcc tgggttctgc
     1801 gcgcgcacgg atccggcgcg gtagaggccg cgcggacatc ggccgactgg tcccggagag
     1861 ccgaggcgcc gtggtggctg gcgcgcgcca tccgggccct gccggacggg gaggcgacag
     1921 ccgaggagct ggccgaggcg gcggagatcg agggcaggct ccgagtccgg gagggagcga
     1981 gcgccccgcc agtctgagcc ctattcgtcg ggcaggccga cggtcgccag cgtttcgggg
     2041 gatatcggtc ccaggcccag acgctcgacc agccacgcgg cgcggtccgc ggtggcggcg
     2101 tcgtccaggg cgtgttccgc ctcgtaggtg gagagcgtcg cgtcgggcac cgcctcgatg
     2161 agctcctgcg ccacgtcggg cgggaccacc tcgtcgatcg tgccgtactg gaggtagatc
     2221 cgctccccgt caagctggtc cgcgatcgcc atggcgtcga agggggccat gacctcgagg
     2281 tagacgtcga acttctcggc accgaccagg gcccgcgcgc cggccaccca gccgtcatcg
     2341 gaggtggcca ggaagccggt ccacgagggt cggggcgcga tgaggatgag ggccgccggg
     2401 cgctcgtcga ccgcggccag ggtggagccg gtcgaggccc cccacgagtg gccgacgtac
     2461 cccagccgcg ccggatccac gtcgtcgcgc tcgaccagga tgtcgtaggc gcggcggacg
     2521 tcgatgatcg cctgggcggt catgtcccgc tcccgctcgg gcagcccgta gctctggagg
     2581 aatgcccgcc gatcggtccc gacccgcgag aacggcgcgt cgagcacgag cgagaccgcc
     2641 ccaccggtgg cc
//
LOCUS       DASYGU010000144         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1361214, whole genome shotgun sequence.
ACCESSION   DASYGU010000144 DASYGU010000000
VERSION     DASYGU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361214"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VGO32_03875"
     CDS             <1..570
                     /locus_tag="VGO32_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199921.1"
                     /translation="GANPLHDDQLPPNSRDRLWSQIQEARMSNNLPQTDSGHTRFARW
                     TIAASGAALVAVVAAALTIGGTAAPPQTGNEGTGGGGGIMMCLAFTIEDLQARDFAFD
                     GTVTAVSGNLATFTVNDGFWGAEDGASVTLQADLMTGEPQQVALEGGPLLEVGSRYLV
                     SGDDDIAWSCGYTQVYDEATAAEWAAAAP"
     gene            complement(634..1113)
                     /locus_tag="VGO32_03880"
     CDS             complement(634..1113)
                     /locus_tag="VGO32_03880"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7199922.1"
                     /translation="MATEISAMDDATRDDDLVIRKTLTVRLSQRDAFRLFTEGIGTWW
                     PTLTHSVAGEVARAGMDDRVGGEIYEESPDGKVTWGTITAWDAPETFSSTWHPGYGAE
                     LATQLTVRFHADGPSTTRVELEHRGWKVHGEDAEESFRGYTVGWDPVLATYVERAGG"
     gene            complement(1116..1427)
                     /locus_tag="VGO32_03885"
     CDS             complement(1116..1427)
                     /locus_tag="VGO32_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007254525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7199923.1"
                     /translation="MPSALDALGALGDPTRRRVFERLLGGPLAVNQIADGMPVTRPAV
                     SQHLRVLVGAGLVTVRSRGTRRVYAVSPEGVGALRAWMDEFWGRALEGFRAAAEQLAE
                     E"
     gene            1572..2867
                     /locus_tag="VGO32_03890"
     CDS             1572..2867
                     /locus_tag="VGO32_03890"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199924.1"
                     /translation="MTRFGQSLRENLGALEERNFRLLWLGQTGSTIGDGLSFVAIAFA
                     VLQIGGTATDIGIVFAAFFLPNVIFLLVGGVWADRLPRNLVMLTSDGVRAVVQIGLAI
                     LMFTGNAQVWQIVIAVALHGTASAFFVPASVGLMPQVLSPGRLQQGNALMAISRSAAF
                     VIGPAVSGLLVAAGGPGVVFAVDAVTYLFSIVTLAMLRVPKAVTAVVRESFVSELAHG
                     WREVRSRDWLIGSLVVFGVSNMAMGAFMVLGPVIFERELGGAAAWGVVMTAGALGALL
                     GGAVALRWKPLRPLAVGFALMLIGASRALSLIPPLPVLVIALSALATAVAIQISNTLW
                     ETVLQQRIPQASLSRVSSYDWMVSLAFQPIAFVVIGPLADTIGEVQTLFISASIGLAA
                     NLVALMVPSIRNMRRLDTDEAAEPSGELSQPASTGNPVP"
     gene            2897..3097
                     /locus_tag="VGO32_03895"
     CDS             2897..3097
                     /locus_tag="VGO32_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199925.1"
                     /translation="MTWEQAADVANPVLKCPLCGGLEFDEEQGRLDSRWGITSHKLVL
                     RICTNCGLVLQFFAGRGIFDFD"
     gene            3144..3812
                     /gene="msrA"
                     /locus_tag="VGO32_03900"
     CDS             3144..3812
                     /gene="msrA"
                     /locus_tag="VGO32_03900"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV7199926.1"
                     /translation="MFSFLSGRKSTMPDPAKALPGRGTALPTAERHRISGQPLKGPYP
                     EGSEIADFAAGCFWGVEKTFWNVPGVIVTAVGYQGGYTPNPTYEEVCSGETGHAEAVR
                     VVFDPSRVSYEQLLKVFWEEHDPTQGMRQGNDTGTSYRSAIYTHGDAQRVAAEASRDM
                     YGAELRKAGYGPITTEIQEAGPFYFAEDYHQQYLDRNPNGYCPVHSTGVLLPGEAVEQ
                     LEQV"
     gene            complement(3827..>4145)
                     /locus_tag="VGO32_03905"
     CDS             complement(3827..>4145)
                     /locus_tag="VGO32_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199927.1"
                     /translation="LNYGRVVEIWSAISVSVGLIALIALLVLTDLPVWAVVLLAFGGY
                     FVLEAAFRRQLINLLLRVALVLALVGAVVLAVDYAGELVILAVLGVALMTLVDNVREI
                     RAG"
BASE COUNT          651 a         1443 c         1386 g          665 t
ORIGIN      
        1 ggagcgaatc cgctccacga cgaccagctg cccccgaaca gccgggaccg actgtggtcc
       61 caaattcagg aggcacgcat gtccaacaac cttccacaga ccgattccgg tcatacccgc
      121 tttgcccgct ggacgattgc cgcctccggc gcggccctcg tcgcggtggt cgcggcggca
      181 ctcaccattg gcggaaccgc cgcgccgccc caaacgggca acgaaggcac tggtggtgga
      241 ggcggcatca tgatgtgcct ggccttcacc atcgaggatc tccaggcccg cgacttcgcg
      301 ttcgacggga ccgtcaccgc ggtcagcggc aacctggcca cgttcaccgt gaacgacggc
      361 ttctggggtg ccgaggacgg cgcgtcggtg acgctccagg ccgacctcat gaccggcgaa
      421 ccgcagcagg ttgcgctcga gggcggaccg ctcctcgaag tgggcagccg ctacctcgtg
      481 tccggcgacg acgacatcgc gtggtcctgc ggctacaccc aggtctacga cgaggccacc
      541 gccgccgagt gggccgcggc cgcgccctga cggcgagcct ctgaatcgtc gagccccgag
      601 cgccggcggc ggagcgggat cgggaaacct gcttcagccg ccggcgcgtt ccacgtaggt
      661 ggcgagcacc ggatcccagc ccaccgtgta gccgcggaac gattcctcgg cgtcctcgcc
      721 atggaccttc cagccgcggt gctcgagctc gacccgggtc gtgctcgggc cgtcggcgtg
      781 gaagcggacc gtcagctggg tggcgagctc cgcgccgtaa ccgggatgcc aggttgacga
      841 gaacgtctcc ggcgcatccc aggcggtgat cgtcccccac gtcaccttcc cgtcggggct
      901 ctcctcgtag atctccccgc cgacccgatc gtccatcccc gcgcgggcca cctccccggc
      961 gaccgagtgg gtgagtgtcg gccaccacgt cccgatcccc tcggtgaaca gccggaacgc
     1021 gtcgcgttgg gaaagccgca ccgtgagggt cttgcggatg acgaggtcgt cgtcgcgtgt
     1081 cgcatcgtcc attgccgaaa tctcggtcgc catggtcatt cctccgctag ttgttcggcc
     1141 gcggcccgga atccctcgag cgcgcggccc cagaactcat ccatccatgc ccgcagggcg
     1201 ccgacgccct ccgggctgac cgcgtagacg cggcgcgtac cccgcgatcg gaccgtcacc
     1261 agccccgccc cgaccagcac ccggagatgc tgcgagacgg cgggccgagt gaccggcatg
     1321 ccatcggcga tctggttgac cgccagcggc ccgccgagca gtcgctcgaa gacccggcgc
     1381 cgggtgggat cccccagcgc acccagggca tcgagcgccg atggcagggc aacgccatcg
     1441 gtcgagccgg atcggggtcg ggatcgggat cggttagcca ccactaaccg ttagtatgta
     1501 cttaccgatg cttcctgtca agcccacccg agccggacaa cggccgacct gccgcctatc
     1561 atcggcaggc gatgacgcgc ttcgggcaaa gcctgcgtga gaacctcggc gccctcgagg
     1621 aacggaactt ccggctcctg tggctgggcc agacgggctc cacgatcggc gacggccttt
     1681 cgttcgtggc catcgccttc gccgtgctcc agatcggcgg aacggcgacg gacatcggaa
     1741 tcgtgttcgc ggcgttcttc ctgcccaacg tgatcttcct gctggtcgga ggggtatggg
     1801 ccgatcggtt gcctcgcaac ctggtgatgc tgaccagcga cggggtgcgg gccgtggtcc
     1861 agatcggcct ggccatcctc atgttcacgg gcaatgccca ggtctggcag atcgtgatcg
     1921 cggtggcgct gcacggaacg gcatcggcct tcttcgtgcc cgcatcggtt gggctcatgc
     1981 cccaggtgct cagcccgggc cggctgcagc agggaaacgc attgatggcc atttcgcgca
     2041 gcgccgcgtt cgtgatcggc ccggcggttt cgggcctgct ggttgcggcc ggaggacctg
     2101 gggtcgtctt cgcggtcgat gcggtgacct acctgttctc gatcgtcacc ctcgccatgc
     2161 tccgggtccc caaggcggtg accgccgtcg tgcgggaatc gttcgtctcc gagctggcgc
     2221 acggctggcg ggaggtccgg tcgcgggact ggctgatcgg ttcgctggtc gtgttcggag
     2281 tctcgaacat ggcaatgggc gcgttcatgg tgctggggcc ggtgatattc gagcgcgagc
     2341 tgggaggggc cgcggcgtgg ggcgtggtca tgaccgcggg cgcgctcggc gccctgcttg
     2401 gaggggcggt ggccctccgc tggaagcccc tccgtccgct ggcggtgggc ttcgccctca
     2461 tgctcatcgg ggcatcccgc gcgctctcgc tcatcccgcc cttgccggtc ctggtgatcg
     2521 ccctctccgc cctggcgacg gccgttgcca tccagatctc gaacaccctg tgggagaccg
     2581 tcctccagca gcggatcccg caggcgtcgc tgtcgcgggt gtcctcgtac gactggatgg
     2641 tgtcgctcgc cttccagccg atcgcgttcg tcgtgatcgg gccgctggcc gacacgatcg
     2701 gcgaggtcca gaccctgttc atttcggcca gcattgggct ggcggccaac ctggtggcgc
     2761 tcatggtgcc gagcatccgc aacatgcgcc gtctcgacac ggatgaagct gccgagccgt
     2821 ctggggagct gtcccagccg gcgagcaccg gcaaccccgt tccctgacct gtaaccctca
     2881 tatcgaagga gtcgccatga cctgggagca ggccgcagac gtcgcaaacc ccgtcttgaa
     2941 gtgtccgctg tgcggcggcc tcgagttcga cgaagagcag ggccggctgg attcacgctg
     3001 gggcatcacc agccacaagc tggtcctccg catctgcacc aactgcgggc ttgtcctcca
     3061 gttcttcgcc ggccgcggga tcttcgattt cgattgatcc ggcggtggcc tgcagcgaca
     3121 ggcggcgtga gtagactgag gacatgttct ccttcctgag cggtcgcaag tcgaccatgc
     3181 ctgatcccgc caaggccctc ccgggccgcg gtaccgccct ccccactgcc gagcgccacc
     3241 gcatcagcgg ccagccactg aaggggccgt accccgaggg ttccgagatc gcggacttcg
     3301 cggccggctg cttctggggc gtcgagaaga cgttctggaa cgtgcccggc gtgatcgtga
     3361 ccgccgtcgg ctaccagggc gggtacaccc cgaacccgac ctacgaagag gtgtgctccg
     3421 gcgagacagg ccacgcggag gccgtgcggg tggtcttcga ccccagccgg gtcagctacg
     3481 aacagctcct gaaggtcttc tgggaggagc acgacccgac gcagggcatg cgccagggaa
     3541 acgatacggg gacctcgtat cggtcagcca tctacaccca tggcgatgcg cagcgggtgg
     3601 cggcggaggc atcgcgcgac atgtacgggg ccgagctgcg caaggcgggc tatggaccga
     3661 tcacgaccga gatccaggag gcgggcccgt tctactttgc cgaggactac caccagcagt
     3721 acctcgaccg gaacccgaac ggctactgcc ccgtgcactc gaccggcgtg ctcctgcccg
     3781 gcgaggccgt ggagcagctg gagcaggtct aggaccacgc gggcgactaa cccgcccgaa
     3841 tctccctgac gttgtcgacc agggtcatga gcgcgactcc gagcacggcc aagatgacca
     3901 gctctccggc gtagtccacg gccaacacca ccgcgccgac cagggccagg accaacgcga
     3961 cgcggaggag caggttgatc agctggcggc ggaatgcggc ctcgaggacg aagtacccgc
     4021 caaacgcgag caggaccacc gcccacaccg ggagatcggt caacaccagg agcgcaatta
     4081 gcgcgatgag gccaacgctg acgctgatgg ccgaccagat ctcgacgacc cgcccatagt
     4141 tcaga
//
LOCUS       DASYGU010000145         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1619356, whole genome shotgun sequence.
ACCESSION   DASYGU010000145 DASYGU010000000
VERSION     DASYGU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619356"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /gene="dnaE"
                     /locus_tag="VGO32_03910"
     CDS             <1..2025
                     /gene="dnaE"
                     /locus_tag="VGO32_03910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV7199928.1"
                     /translation="AVELIGRHRGVAIDVDNLPLDDTKTFELLSTGETTGIFQLEGSG
                     MRRYVKDLRPTEVRDLAAMVALFRPGPMANIPAYIRRKHGEEAVTYLHPALEPALRDT
                     YGIFVYQEDIMAAAIAMADYTGPEADNLCYAIRKKNAQVLREHEAKFKAGAKKKGIPP
                     GVVDQVFAAFEPFARYGFNKAHATCYGLIAYQTAYLKANYPVEFMTAVLNGFRERAEK
                     VAAVVAECRRLGIEVRPPDVQASHALFEVETDADGTRAIRWGMAAVKNVGEGAVESIV
                     AAREAETDGGPFTSLDDLCRRVDQQRVNKRVFESLIKVNALVSLGPMAALLDRLDSAL
                     DAAARHHRDVAAGQSTMFDLFAVPMAIEAPVNGDLAADGRGEISRRERLRWEKELLGL
                     YLTEHPLGDIADELPSYVTAYTGDLAEESDQSRVTLGGIIQGTRRVITRAGSTMLVVQ
                     LEDLQGSVEIVVFPKVFTETANAWVEDAVVLVSGRVDHRDEEAKLLCDTVHAWDDAQR
                     LGPAAYGAERDRLNRNRSGRPPGPSDPLRPSIGPSAEVPVARPRGAEPLAPAVAASEE
                     TPAPRDARPIPAVPEGAAGSLWISFAPDLSSDALLDAIESVTSIVRQRPGDLPVVLAV
                     PVAGATRQVRLPQATGWDEEIEAVLRREVTAELSVERRPESAIPSVSSGA"
     gene            2042..3253
                     /locus_tag="VGO32_03915"
     CDS             2042..3253
                     /locus_tag="VGO32_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7199929.1"
                     /translation="MTRQIRRAMTGLRDRFMVEGSSADIGADMSLALPPDELEVPPEP
                     MGPVAVTLRVVGVVVAVALVLGLLWLLRGLVLNVFLALLFAAGLLGAARGFEQRMSRA
                     AAALTVNAIAIGVVIVVILLAARPAMDAIGSFVQAIPGIVDSFSAWIEGLLGSDAYDQ
                     LFTDEVDVSFLLGGILDVTLATIAVIANLVVIVIIEIFLLIERRPLLDGVLEFMDSED
                     RPATRYILTTGIDSLGKYLRGLIVSMAFIGVATAVGMWLIGIPYAIPMGIIAFFTAAI
                     PYIGGLFALVPILIIAGVEVGPTAVLLMIGWQIIIQQIEGSVVTPTVQGKAINISPLV
                     VMLGVTAGITLGGLVGGIIAIPIAALIGVGIRGVVIPMRRRAEAREQARRAAYLAARQ
                     AAIEAAAADPA"
     gene            3278..3742
                     /locus_tag="VGO32_03920"
     CDS             3278..3742
                     /locus_tag="VGO32_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199930.1"
                     /translation="MTAPRKPASPKAASAKTAPVKTTPAKAAPTGDDLTAAILRYLRR
                     VPNEERPLDPLAEELGMDPAALQLAVERLHWRRYLIAPFVEPGRAGGAKLTAVGLHWL
                     IAREGGKPRDTPVAFQPARRRVRTQDEAARLPRATVYGVRGAATSTSAAPER"
     gene            complement(3739..4557)
                     /locus_tag="VGO32_03925"
     CDS             complement(3739..4557)
                     /locus_tag="VGO32_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV7199931.1"
                     /translation="MSQPSSAHISHPVDTSRLGEPIVQAIGIEKYFGDHHVLRGVDLT
                     VRQAEAVMLIGRSGSGKTTFLRCVNFLEEATVGSVEIDGVKVEADPLKPRSRAHQEQI
                     RLIRMRAGMVFQDFNLFPHMTVLDNCIEAPVRVAKVSKAEATERAEKYLAKVGLAEKR
                     DEYPARLSGGQKQRVAIARALSMEPRVLLFDEPTSALDPELIGEVLKVMEELAHEGAT
                     MIVVTHELHFAREAADRVVFLEEGRVIEEGPPEKMLDDPDDDRTRQFLRRFLTN"
     gene            complement(4554..>5366)
                     /locus_tag="VGO32_03930"
     CDS             complement(4554..>5366)
                     /locus_tag="VGO32_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7199932.1"
                     /translation="MTAAAPPVSAPAPILEQVKAANSRRKLTFRLWFWFTWFALVGAL
                     LALLYATGNIDTDFITSAGPYIIGGAGITIVISLTSITFATGLALVGALGRLSRNPIF
                     NGIASLYVSLVRGTPLLVQIFFVYLALPQLGIVLPVIVGGIFALAFNYGAYMTEVFRA
                     GIQAVPKGQREAAQALGMPEGLIMRRIVLPQAFRIVTPAIGNDFVSMIKDSSLVYVLG
                     VQELLWRASTIGRRELSSLEALLIAAAVYWGMTIIFSYFQERIEKRMARGDR"
BASE COUNT          798 a         1861 c         1856 g          851 t
ORIGIN      
        1 gcggtcgagc tcatcggtcg tcaccgcggg gtcgcgatcg acgtcgacaa cctgccgctg
       61 gacgacacga agacattcga gctgttgtcg acgggggaga cgaccggcat cttccagctc
      121 gagggctcgg gcatgcgccg gtacgtcaag gacctgcgtc cgacggaggt ccgtgacctc
      181 gcggccatgg tcgccctctt ccgtcccggg ccgatggcca acatcccggc ctacatccgg
      241 cgcaagcacg gcgaggaggc ggtgacgtac ctccacccgg ccctggagcc ggccctccgg
      301 gacacgtacg ggatcttcgt gtaccaggag gacatcatgg ccgcggccat cgcgatggcc
      361 gactacaccg gccccgaggc cgacaacctg tgctacgcga tccggaagaa gaatgcccag
      421 gtcctccgcg agcacgaggc caagttcaag gccggtgcca agaagaaggg catcccgccc
      481 ggcgtcgtgg accaggtgtt cgccgccttc gagccgtttg cccgctacgg cttcaacaag
      541 gcgcatgcca cctgctacgg gctgatcgcc taccagactg cctatctcaa ggcgaactac
      601 ccggtcgagt tcatgaccgc ggtcctgaac ggcttccgcg agcgggcgga gaaagtggcc
      661 gcggtggtgg ccgaatgccg gcggctgggg atcgaggtcc gcccaccgga cgtccaggct
      721 tcccacgcgc tgttcgaggt ggagaccgat gcggatggca cccgcgccat ccggtggggc
      781 atggcggcgg tgaagaacgt gggcgagggt gcggtggaga gcatcgtcgc cgcccgcgag
      841 gccgagaccg atggcggtcc cttcacctcc ctcgacgacc tctgccgtcg cgtggaccag
      901 cagcgggtca acaagcgcgt gttcgagtcg ctgatcaagg tcaacgccct ggtatcgctc
      961 gggccgatgg ccgcgctgct cgaccggttg gactccgcgc tcgacgctgc cgcgcggcat
     1021 caccgggacg tggccgccgg ccagtccacg atgttcgacc tgttcgcggt gccgatggcc
     1081 attgaggcgc cggtgaacgg cgacctggcc gcggacggcc ggggcgagat ctcacgccgc
     1141 gagcgcctcc gctgggagaa ggagcttctg ggcctgtacc tgaccgagca tccgctgggc
     1201 gacatcgccg acgagctgcc ctcgtacgtg accgcctaca ccggcgacct ggccgaggag
     1261 tcggaccagt cgcgcgtgac cctgggcggg atcatccagg gcacgcggcg ggtgatcacc
     1321 cgtgccggct cgacgatgct cgtcgtccag ctcgaggacc tccaggggag cgtcgagatc
     1381 gtcgtcttcc ccaaggtgtt taccgagacc gcgaacgcgt gggtcgagga cgcggtggtc
     1441 ctggtcagcg gccgggtgga ccaccgcgac gaggaggcga agctcctgtg tgacacggtc
     1501 cacgcctggg atgacgccca gcgcctgggc ccggccgcct acggcgccga gcgcgaccgc
     1561 ctgaaccgca accgctccgg ccgcccgccg ggcccgtcag acccgctccg tccgtccatc
     1621 ggcccatcgg ccgaggtccc ggtcgcacgg ccgcggggtg ccgagccact cgccccagcg
     1681 gtcgccgctt ccgaggagac accggcgccc cgcgatgcgc ggccgatccc cgccgtcccg
     1741 gagggcgcgg ccggctcgct gtggatctcg ttcgcgcccg atctgtcctc ggacgcgctg
     1801 ctggacgcga tcgaatcggt cacctccatc gtccggcagc gacccggcga tctgccggtc
     1861 gtgctggccg tgccggtggc gggcgccacg cgccaggtcc gactcccgca ggcgactggc
     1921 tgggacgagg aaatcgaggc tgtcctgcgc cgagaggtga ccgccgagct gagcgtggaa
     1981 cgccgccccg agtccgcgat cccatcagta tcatccggag cgtgacggcg acccgatcgc
     2041 catgacccgg cagatccggc gcgccatgac cggcctccgc gaccggttca tggtcgaggg
     2101 ctcgtcggcc gacatcggcg ccgacatgtc actggccctg ccaccggacg agctcgaggt
     2161 tccgcccgag ccgatgggcc cggtcgcggt cacgctccgc gtcgtgggcg tggtggtcgc
     2221 cgtggccctt gtcctcggcc tgctctggct cctccgcggt ctcgtcctga acgtgttcct
     2281 ggctctcctg ttcgcggccg ggctactcgg ggccgcgcgt gggttcgagc agcggatgtc
     2341 ccgcgcggcg gctgccctca cggtcaacgc gatcgccatc ggcgtcgtga tcgtcgtcat
     2401 cctgctcgcg gctcgcccgg ccatggacgc gatcggatca ttcgtgcagg ccattcccgg
     2461 catcgtcgat tcgttctcgg cgtggatcga gggcctgctc gggagcgacg cctacgacca
     2521 gctgttcacc gacgaggtgg atgtgtcgtt tctgctggga ggcatcctgg acgtcacgct
     2581 cgccacgatc gcggtcatcg ccaacctggt cgtcattgtg atcatcgaga tcttcctgct
     2641 catcgagcgc aggcccctgc tcgatggcgt gctcgagttc atggactccg aggaccggcc
     2701 ggcgacccgc tacatcctca ccaccggcat cgacagcctg ggcaagtacc tgcgcgggct
     2761 gatcgtctcg atggcgttca tcggcgtggc gaccgcggtg gggatgtggt tgatcgggat
     2821 cccatacgcg ataccgatgg ggatcatcgc gttcttcacg gccgcgatcc cgtacatcgg
     2881 cgggctgttc gcgctcgttc cgatcctgat catcgccggg gtcgaggttg gtccgaccgc
     2941 ggtcctgctc atgatcggct ggcagatcat catccagcag atcgaggggt cggtggtcac
     3001 gcccacggtc cagggcaagg cgatcaacat ctcgccgctc gtcgtcatgc tgggcgtgac
     3061 cgcgggcatc accctcggtg gcctcgtggg cgggatcatc gcgatcccca tcgccgcgct
     3121 gatcggtgtg ggtattcggg gtgtcgtgat tcccatgcgg cggcgggcgg aggctcgcga
     3181 gcaggcccga cgggccgcct atctcgccgc ccggcaggcc gcgatcgagg cggcagccgc
     3241 tgatcccgcg tagcggcgcc tcggtatcat cggcagcgtg accgcgccgc ggaagcccgc
     3301 ttctccgaaa gccgcgtccg cgaagacggc gccggtgaag accacgcccg cgaaggctgc
     3361 cccgaccggg gacgacctca ccgcggcgat cctccgctac ctccgtcgtg tcccgaacga
     3421 ggagcggcca ctggacccgc tggccgagga gctgggcatg gatccggcgg cgctccagct
     3481 cgccgtggag cggctccact ggcgccgcta cctcatcgcc ccgttcgtgg agcccgggcg
     3541 tgccgggggt gccaaactga ccgcggtcgg gctgcactgg ctcatcgcgc gcgagggcgg
     3601 gaagccgcgc gacacgccgg ttgcgttcca gccggcccgc aggcgcgtcc gcacccagga
     3661 cgaagcggcc cgcctgccgc gcgccacggt ctatggcgtc aggggcgccg cgacgtctac
     3721 ttccgccgcg ccggagcgct agttcgtcag gaagcgcctc aggaactggc gggtccggtc
     3781 gtcatcgggg tcgtccagca tcttttcggg cggcccttcc tcgatcacgc gcccctcctc
     3841 caggaagacg acccggtcgg ccgcctcgcg ggcgaagtgc agctcgtggg tgacgacgat
     3901 catcgtcgcg ccctcgtggg cgagctcctc catgaccttc agcacctcgc cgatgagctc
     3961 ggggtccagc gcgctggtcg gctcgtcgaa gaggaggacc ctgggctcca tcgacagcgc
     4021 ccgggcgatg gccacccgct gcttctggcc accggacagg cgagcggggt actcgtcgcg
     4081 cttctcggcc agccccacct tggcgaggta cttctccgcc cgctcggtgg cctcggcctt
     4141 gctcaccttg gcaacccgca cgggggcctc gatgcagttg tccaggacgg tcatgtgggg
     4201 gaacaggttg aagtcctgga acaccatccc ggcccgcatc cggatcaggc ggatctgctc
     4261 ctggtgggcg cggctgcgcg gtttgagcgg atccgcctcc accttgacgc cgtcgatctc
     4321 gaccgatccg acggtcgcct cctcgaggaa gttcacgcac cgcaggaagg tcgtcttccc
     4381 gctcccggac cggccgatga gcatgaccgc ttcggcctgg cggacggtga ggtccacgcc
     4441 gcgcaggacg tggtggtccc cgaagtactt ctcgatcccg atcgcttgca cgatcggctc
     4501 gccgagccgg ctggtgtcga ccgggtggct gatgtgcgcg gatgatggct ggctcatcgg
     4561 tcacctcggg ccatgcgctt ctcgatgcgc tcctggaagt agctgaagat gatggtcatg
     4621 ccccagtaca ccgccgccgc gatgagcagc gcctcgaggc tggagagctc gcgacgccca
     4681 atggtggatg cccgccacag cagctcctgg acaccgagga cgtaaaccag cgagctgtcc
     4741 ttgatcatcg acacgaagtc gttgccgatg gccggcgtca cgatccggaa ggcctggggc
     4801 aggacgatcc gtcgcatgat caggccctcg ggcatgccga gcgcctgcgc cgcctcccgc
     4861 tggcccttgg gcacggcctg gatgccggcc cggaagacct cggtcatgta cgcgccgtag
     4921 ttgaacgcga gggcgaagat gccacccacg atcaccggca gcacgatccc gagctgaggc
     4981 agcgcgaggt agacgaagaa gatctggacc aacagcggcg tgccccgcac gagcgatacg
     5041 tagagcgacg cgatcccgtt gaagatcggg tttcgtgaca gccgaccgag ggcgccgacg
     5101 agcgcgaggc cggtagcgaa cgtgattgac gtcagcgaga tcacgatcgt gatccccgcg
     5161 ccgccgatga tgtacggacc ggcggacgtg atgaagtcgg tgtcgatgtt cccggtcgca
     5221 tacagcagtg cgagcagggc gccgacaagg gcgaaccagg tgaaccagaa ccagagccgg
     5281 aacgtcagtt tgcgacggct gttcgccgcc ttgacctgct cgaggattgg cgccggggct
     5341 gataccggcg gggcagcggc ggtcat
//
LOCUS       DASYGU010000146         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_75869, whole genome shotgun sequence.
ACCESSION   DASYGU010000146 DASYGU010000000
VERSION     DASYGU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75869"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGO32_03935"
     CDS             <1..344
                     /locus_tag="VGO32_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEV7199933.1"
                     /translation="VFYHGAYKPPREWNWAVGTFLLFCTLWLSFTGYLLPWDQIAFWA
                     VTVGTQMAAYAPLVPVESNLILLGAVEVGQETLIRFYVFHVIAFPLITAIFMVIHFWR
                     IRKDGGISGPV"
     gene            358..978
                     /locus_tag="VGO32_03940"
     CDS             358..978
                     /locus_tag="VGO32_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEV7199934.1"
                     /translation="MTIEKRPNATADKNAPTQAPVPTTPPAVPGQGPKLPGAPGAPGT
                     ERFRLLAYVRQESMVRVDKRPDETVLTWPHLLRAEFLMACLITAMLLVMSFFVMAPLE
                     EQATVNVTPHVAKAPWYFLGLQEILAYFNPTVAGIFIPPIYFIGLALIAYLDRSPYRA
                     ARDRKVAWLFFLCIMFFGIGVTVIGAFFRGPGWNWVWPWDGLFFTL"
     gene            989..1609
                     /locus_tag="VGO32_03945"
     CDS             989..1609
                     /locus_tag="VGO32_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7199935.1"
                     /translation="MTNQPEGWGEQPGPPVREISRRGFLRRAVGVGVGLLTLQFVGGT
                     LLFLWPNLKGGLGDPNLVIGSAADITGQQPEWVRGVPFIFNKARLFIVNVPAAKALVD
                     GTGEEFDDPGMDILAMYRKCPHLGCNIPQLCDRSKWFECLCHGSKYNIIGEKRAGPAP
                     RGMDRFAHRIEDGVYIVDTSVKISGPPIGTATFDNRLTEDIEHCAA"
     gene            1626..2711
                     /locus_tag="VGO32_03950"
     CDS             1626..2711
                     /locus_tag="VGO32_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7199936.1"
                     /translation="MKALGVALMVGFAAFTTFYWITDAPRRDASEVVDDEERIEFGRL
                     VFLPDDTFTVIVAVTPDGFFQKVDEELVPLTTLDVFVNTYVSFENDSGSAVDVSGSGI
                     EVFDETIEDGANIAVRFTSEGETEATVPGLEEPLVISAGPPHLQPYGANCARCHGVDG
                     QGLIGPNLHSLRLATKWSQTGGAQGLNNYVAWVITLGGVVVSGNVNSIMPAWGQDYGG
                     TLTRQQIEALTAMIGGWAQETLDNPPPSPVEVPNTVEAGAEVYAAAGCVGCHGADLSG
                     GVGPNIQGIGTSLVTDGLTTQTLDIEQMQADYDENPRAFLETWIRTSSVYNNGTPTGM
                     PEFDETDIGDSQLQALITFLLEGDHTP"
     gene            2708..3283
                     /locus_tag="VGO32_03955"
     CDS             2708..3283
                     /locus_tag="VGO32_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199937.1"
                     /translation="MTSYALPRRRGLLARLIGEADDFTTWLLFGSETWLIAAMKAVPL
                     FLFIYWLFTYVPNSVYYGVTQYIPFLQFSAEVGFIVANVVAGTNVLLVVVLAFVVQAS
                     RGRQGPGWTLLRLFVLANYVAVVLLLIPYFVFNVAGGSFIPLELPLIALALGAMTAGM
                     GVLALGYLYYEFRRISRREAQAAAALSVRAG"
     gene            3373..3669
                     /locus_tag="VGO32_03960"
     CDS             3373..3669
                     /locus_tag="VGO32_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199938.1"
                     /translation="MAAAAIIGRELVASCPPGAWLAIDCGPTRDFVLSVIALGAVLYV
                     ALLSAVVWWSGRLADRPGSDPNGGRDWYVVAALVGVVIAPLLAFTILAALGWLG"
     gene            3680..5128
                     /locus_tag="VGO32_03965"
     CDS             3680..5128
                     /locus_tag="VGO32_03965"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEV7199939.1"
                     /translation="MTAAPSDALRSSLGLAATITGDRLDVLDPGAFRGPLTDRLVRDA
                     VFAAAPATRDAARWAIWAASQALGCPSASIHDLYVARGRGDVPADAFTVPAINVRATT
                     YLTARTVFAVARRREVGAVVLEIAKSEMAYTEQRPDEYAAVVLAAALREGWTGPVFLQ
                     GDHFQFNATRWQADPDAEVNGLHDLAREAVRAGFLNIDIDASTLVNLDRSTIADQQWV
                     NAERTADLTRLIRGVQPGGVTISIGGEIGEVGKANSTEAELRAFLGEYGAALGAEEGI
                     AKVSIQTGTSHGGVVLPDGSVAPVALDFDTLGRLSTVAREEYGLAGCVQHGASTLPEE
                     AFGHFPIAGCAEIHLATGFQNILFDGGGLPADLRAEMMAWCLVNCADERKPGETDEQF
                     LYKTRKKALGPFKAALWDIGAEAEAEIGAHLAARLDVLFERLGADGTRDVVDRWVQPP
                     DLPRPMPAALGGTGGPGVLTGQDTFADDGSGE"
     gene            5134..6003
                     /locus_tag="VGO32_03970"
     CDS             5134..6003
                     /locus_tag="VGO32_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7199940.1"
                     /translation="MANSQRKSLDLPDEERAVPNGKVEIWNLGDFVIGRATFNPGWRW
                     STDVKPIAGTEWCEYHHLGLMLEGEMHIATADGLGMELKVGHIYEVLPGHDAWVVGEG
                     RVVQYDFAGMRTFALPAAGRAERVLSTLICTDIVDSTATAERIGPSVWTSKLAELNAA
                     SRHEIDRFRGRLAATTGDGVIAMFDGAERAIRCAAAISERAAAQGLDVRVGVHTGEVE
                     LLPDDIRGVAVHVLSRVTALGGAGEVLVSGTTHELVVDSDLRFEDRGQHELKGVTSAR
                     QIWVLLLDGSAAR"
     gene            complement(6061..6429)
                     /gene="erpA"
                     /locus_tag="VGO32_03975"
     CDS             complement(6061..6429)
                     /gene="erpA"
                     /locus_tag="VGO32_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEV7199941.1"
                     /translation="MTEQLVSLTDAAAAKVMELRSREGKAEASLRLFVKSGGCSGFSY
                     GLAFDDSRAEDDRVEFHGEVPIVIDAFSAQYVDGAEIDYVDSLMGSGFAINNPNAVSS
                     CACGSSFNTDGSPSKAGGCH"
     gene            6574..>7395
                     /locus_tag="VGO32_03980"
     CDS             6574..>7395
                     /locus_tag="VGO32_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7199942.1"
                     /translation="MKLAERMSRIGTETAFEAAARARALEATGRSVIHLEIGEPDFDT
                     PQHIKDAAARGLLEQGMTHYTAAEGIAPLRQAIADDVLRWKGIEATPEQVIVTPGAKP
                     IMFYAMLALIDEGDEVIYPNPGFPIYESMTEYVGGVAVAAPLREERDFRMDVEETASL
                     VTPRTKMIVFNSPHNPTGSILTEDDVRAIADIANRHDLVVLADEIYGRLQYEGEPLSI
                     ATLPGMAERTITLDGFSKTFAMTGWRLGYGIIPSWMVPTYSRLVINSVSCTNAFAQ"
BASE COUNT         1145 a         2536 c         2494 g         1220 t
ORIGIN      
        1 gggtcttcta ccacggggcc tacaagccgc cgcgcgaatg gaactgggcg gtgggcacgt
       61 tcctcctgtt ctgcaccctg tggctgagct tcaccggata cctgctgccg tgggaccaga
      121 tcgcgttctg ggccgtcacg gtcgggaccc agatggccgc ctacgccccg ctggtgccgg
      181 tcgagtcgaa cctcatcctc ctcggcgcgg tcgaagtcgg ccaggagacg ctcatccgct
      241 tctacgtgtt ccacgtgatc gccttcccgc tcatcaccgc catcttcatg gtcatccact
      301 tctggcggat ccggaaggat ggcgggatca gcggaccggt ataggacgga ctgaccgatg
      361 accatcgaga agcgaccaaa cgccaccgcg gacaagaacg cgcccaccca ggcgccggtc
      421 cccaccacgc cgccggccgt tcccggccag ggcccgaagt tgcccggcgc gcccggcgcg
      481 cccggcaccg agcggttccg gctgctggcc tacgtgcgcc aggaatccat ggtccgggtg
      541 gacaagcggc ccgatgagac ggtcctgacc tggccgcacc tgcttcgcgc cgaattcctc
      601 atggcctgcc tgatcacggc catgctcctg gtcatgtcgt tcttcgtcat ggcgcccctg
      661 gaggagcagg cgaccgtcaa cgtcaccccg cacgtcgcca aggcgccgtg gtacttcctc
      721 ggcctgcagg agatcctggc ctacttcaac ccgaccgtgg ccggcatctt catcccgccg
      781 atctacttca tcggcctggc cctgatcgcc tacctggacc gctccccgta ccgcgcggcc
      841 cgtgaccgga aggtggcctg gctcttcttc ttatgcatca tgttcttcgg catcggggtg
      901 acggtcatcg gtgccttctt ccgcggcccc ggctggaact gggtctggcc gtgggacggc
      961 ctgttcttca cgttatgacg gccgtctgat gaccaaccag cccgagggtt ggggggagca
     1021 gcccggaccg ccggtccgcg agatcagccg gcgcggattc ctgcgccgcg cggtcggcgt
     1081 cggcgtgggg ctgctcacgc tccagttcgt gggtggcacc ctcctgttcc tgtggcccaa
     1141 cttgaagggc ggactcggcg acccgaacct ggtcatcggg agcgcggccg acatcaccgg
     1201 acagcagccg gagtgggttc gcggtgtgcc gttcatcttc aacaaggccc gcctgttcat
     1261 cgtgaacgtg cccgccgcca aggctcttgt cgacggtacg ggtgaggagt tcgacgaccc
     1321 ggggatggac atcctggcca tgtatcgcaa gtgcccgcat ctcggctgca acatcccgca
     1381 gctgtgcgac cggagcaagt ggttcgagtg cctgtgccac ggctcgaagt acaacatcat
     1441 cggcgagaag cgggccggtc cggcgccgcg ggggatggat cgcttcgcgc atcgcatcga
     1501 ggacggggtg tacatcgtgg acacctcggt gaagatcagc gggccgccga tcggcaccgc
     1561 caccttcgac aaccgcctga ccgaggacat cgagcattgc gccgcctagg tgatcgcccg
     1621 tgcgcatgaa ggcgctgggc gtggccctga tggtcggctt cgcggcgttc acgaccttct
     1681 actggatcac cgatgcgccg cgccgtgacg cgtcggaggt cgtcgacgac gaggagcgaa
     1741 tcgagttcgg ccggctcgtc ttcctgcccg acgacacgtt cacggtcatc gtggccgtca
     1801 cccctgatgg ctttttccag aaggtcgacg aggagctcgt cccgctgacc accctggacg
     1861 tgttcgtgaa cacctacgtc agcttcgaga acgacagcgg gtccgcggtc gacgtcagcg
     1921 gcagcgggat cgaggtcttt gacgagacta tcgaggacgg tgcgaacatc gcggtgcggt
     1981 tcacgtccga gggagagacg gaagccaccg tccccggtct cgaggaaccg ctggtcatct
     2041 cggccggacc gccacatctc cagccctacg gcgccaactg cgcgcgctgc cacggggttg
     2101 atgggcaggg cctgatcggt cccaacctcc actcgctgcg cctcgccacc aagtggtcgc
     2161 agaccggtgg cgcccaggga ctgaacaact acgtggcctg ggtcatcacc ctgggcggag
     2221 tcgttgtgtc cggcaacgtg aacagcatca tgccggcctg gggccaggac tacggtggca
     2281 cgctcacccg gcagcagatc gaggccctga ccgcgatgat cggcggatgg gcgcaggaga
     2341 cgctcgacaa cccgccgccc tccccggtcg aggtcccgaa cacggtcgag gccggcgccg
     2401 aggtctatgc cgccgccggc tgcgtcggct gtcatggcgc cgacctgtca ggtggtgtcg
     2461 gtccgaacat ccagggcatc gggacatcgc tggtgaccga cgggctgacc acccagacgc
     2521 tcgatatcga gcagatgcag gcagactacg acgagaaccc gcgcgcattc ctcgagacct
     2581 ggatccggac atcttcggtc tacaacaacg gcaccccgac cggcatgccg gagttcgacg
     2641 agactgacat cggtgattcg cagctccagg ccctgatcac gttcctgctg gagggggacc
     2701 acaccccatg accagctacg ccctgccacg gcgccgcggc ctgctggccc ggctcatcgg
     2761 cgaggccgat gacttcacca cctggctcct gttcgggtcg gagacctggc tcatcgccgc
     2821 catgaaggcc gtgcccctgt tcctgttcat ctactggctg ttcacctacg tcccgaactc
     2881 ggtctactac ggcgtcaccc agtacatccc ctttctccag ttctcggccg aagtcggctt
     2941 catcgtcgcg aacgtcgtgg cggggacgaa cgtcctgctg gtcgtggtgc tcgcgttcgt
     3001 ggtccaggcg tcgcgcggcc gacagggacc cgggtggacc ctgctccgcc tcttcgtgct
     3061 ggccaactac gtcgccgtcg tcctgctcct gatcccgtac ttcgtgttca acgtcgccgg
     3121 cggctcgttc atcccgctcg agctgccgct catcgccctc gccctgggcg cgatgaccgc
     3181 cggaatgggc gtgctcgccc tgggctacct gtactacgag ttccgccgca tcagccgccg
     3241 tgaggcgcag gccgcggccg ccctgtcggt gcgcgcgggc tgaggcccga acgccccgcc
     3301 cacacgcagg tccaggccat ctcccggcgc cggctcctcc tcgttcatct cggctggtcc
     3361 gcgctcgtcc tgttggcggc cgcggccatc atcggtcgcg agctggtggc ctcatgcccg
     3421 ccgggagcct ggctggccat cgactgcggg cctacccgcg atttcgtgct cagcgtgatc
     3481 gctctcggcg cagtgctgta cgtggccctg ctgagcgcgg tcgtgtggtg gtcaggaagg
     3541 ctggccgacc ggccggggtc ggaccccaac ggcggccggg actggtacgt cgtcgcggcg
     3601 ctggtgggcg tggtcatcgc ccccctgctg gccttcacca tcctcgccgc gctcgggtgg
     3661 ctgggctagg atgccaacca tgaccgcggc gccttccgat gcccttcggt cgagcctggg
     3721 actcgccgcg acgatcaccg gcgaccggtt ggacgtcctc gatccgggcg cgtttcgcgg
     3781 cccgctgacc gaccgcctgg ttcgcgacgc ggtcttcgcc gccgctccgg caacccggga
     3841 cgccgcgcgc tgggccatct gggccgccag ccaggcgctt gggtgcccat cggcctccat
     3901 ccatgacctg tatgtggcgc gcggccgagg cgacgtgccc gcagacgcct tcacggtccc
     3961 ggccatcaac gtccgtgcca ccacctacct caccgcccgg acggtgttcg cggttgcccg
     4021 gcggcgcgag gtcggggcgg tcgtgctcga gatcgcgaag agtgagatgg catacaccga
     4081 acaacggccg gacgagtacg ccgcggtcgt cctcgccgcc gcgctccggg aagggtggac
     4141 cgggccagtc ttcctccagg gcgaccactt ccagttcaac gccacccgct ggcaggcgga
     4201 tcccgatgcc gaggtgaatg gcctccacga cctcgctcgt gaagcggttc gcgccggctt
     4261 cctgaacatc gatatcgatg ccagcaccct ggtcaacctc gatcggtcaa cgatcgccga
     4321 ccagcagtgg gtgaatgccg agcggacggc ggacctcacc aggctcattc ggggcgtgca
     4381 gcctggcggc gtgacgatct cgatcggtgg cgagatcgga gaggtcggga aggcgaactc
     4441 gaccgaggcg gagctgcggg cgttcctcgg cgaatacggc gccgccctgg gagccgagga
     4501 ggggatcgca aaggtcagca tccagaccgg cacatcccac ggcggcgtgg tgctgcccga
     4561 cggatcggtg gcgccggtcg ccctggactt cgacaccctg ggccggcttt ccactgtggc
     4621 acgcgaggaa tacgggctgg ccggctgcgt tcagcacggg gcgagcaccc tgcccgagga
     4681 ggcgttcggc catttcccga tcgcggggtg cgccgagatc cacctcgcca ccgggttcca
     4741 gaacatcctg ttcgacggcg gcgggctgcc ggcggacctc cgggccgaga tgatggcctg
     4801 gtgcctggtc aactgcgccg acgagcgcaa accaggggag accgacgagc agttcctgta
     4861 caagacccgc aagaaggcgc tcgggccgtt caaggccgcg ctgtgggaca tcggggcgga
     4921 ggccgaggcg gagattggcg cgcacctggc cgcgcggctg gacgtcctct tcgagcggct
     4981 gggagcggac ggcacgcgcg acgtggtcga ccgatgggtc caaccgccgg atctgcccag
     5041 acccatgccg gccgcgctcg gcggcaccgg tggccccggg gttctgaccg gtcaggacac
     5101 cttcgccgac gacggttcgg gagaataggg gcaatggcca actctcagcg caagtccctg
     5161 gatctgcccg acgaggaacg ggccgtcccg aacggaaagg tcgagatctg gaacctcggt
     5221 gacttcgtca tcgggcgggc caccttcaat cccggctggc gctggtcgac ggatgtgaag
     5281 ccgatcgccg ggaccgaatg gtgcgagtac caccacctcg ggctcatgct cgagggcgag
     5341 atgcacatcg ccaccgccga tggtctgggg atggagctca aggtgggtca catctacgag
     5401 gtgcttccgg gccacgacgc gtgggtcgtc ggcgaggggc gggttgtcca gtacgacttc
     5461 gccgggatgc ggaccttcgc cctgcctgcc gccgggcgag cggagcgggt gctgtcgacg
     5521 ctgatctgca ccgatatcgt cgactccacc gccaccgctg agcggatcgg cccatccgtc
     5581 tggacctcga agctggccga gctcaacgcc gcgagccggc atgagatcga cagattccgc
     5641 ggtcggctcg ccgccacgac gggggatggg gtcattgcca tgttcgatgg tgctgagcgc
     5701 gcgatccggt gcgccgccgc catcagcgag agggctgcag cccagggcct cgatgtccgg
     5761 gtgggcgtcc ataccggcga ggtcgagctc ttgccggatg acatccgcgg cgtcgcggtc
     5821 cacgtcctca gccgggtgac cgcgcttggc ggagcgggcg aggtcctcgt cagcgggacc
     5881 acccatgagc tggtcgtgga ctccgacctc cggttcgagg atcgcggcca gcacgagctg
     5941 aagggcgtca ccagcgcccg tcagatctgg gtgctgctcc ttgacgggag cgcagcccga
     6001 taaggaaacg accccggtgg cgggaccggg gtcgtcaggg aatcgtcgag atacgaaggc
     6061 ctagtggcaa cccccggcct tggacggcga cccgtcggta ttgaacgacg agccacaggc
     6121 gcagctgctg accgcgttcg ggttgttgat cgcgaaacct gagcccatca gggagtcgac
     6181 gtagtcgatc tccgcgccat ccacgtactg ggcgctgaag gcgtcgatca cgatcgggac
     6241 ctcgccgtgg aactcgaccc ggtcgtcctc ggcacgcgag tcgtcgaacg ccaggccgta
     6301 gctgaagccc gagcagccgc cgctcttcac gaagaggcgg agggatgctt cggccttgcc
     6361 ttctcgggag cgcagctcca tgaccttcgc cgcggcggcg tcggtgaggc tgaccagttg
     6421 ctcggtcatc ggcgctgctg ctggggaggt ggtgggagtt gtctcggaaa gcgtcacggt
     6481 tggctgtctc cggcgcgggt tgggaattcg cttcggaaag tatagcaatc tcacgccaca
     6541 cggccgcaat ggcccatcgg tacacttgcc agcgtgaagc tggcggagcg gatgtcccgc
     6601 atcgggacgg agacggcctt cgaggcagcg gcccgggcgc gggccctgga agccaccgga
     6661 cggagcgtca ttcatctcga gatcggtgag cccgacttcg acacgcccca gcacatcaag
     6721 gatgcggccg cgcgcgggct cctggagcag ggaatgaccc actacaccgc ggccgagggc
     6781 atcgccccgc tgcggcaggc catcgccgac gacgtgctcc gctggaaggg aatcgaggcc
     6841 acgcccgagc aggtgatcgt gacccccggc gcgaagccga tcatgttcta cgccatgctc
     6901 gccctgatcg acgagggcga cgaggtcatc taccccaacc ccggcttccc gatctacgag
     6961 tcgatgaccg agtacgtggg cggggtggcg gtcgcggccc ccctccgcga ggagcgcgac
     7021 ttccggatgg acgtcgagga gactgcctcg ctggtcacgc cgcggaccaa gatgatcgtg
     7081 ttcaacagcc cgcacaaccc gaccggctcg atcctgaccg aggacgacgt gcgggcgatc
     7141 gccgacatcg cgaaccgcca cgacctggtc gtcctggccg atgagatcta cggccgcctc
     7201 cagtacgagg gcgagccgct ctcgatcgcg accctgcccg gcatggccga gcgcacgatc
     7261 acgctggacg ggttctcgaa gacgtttgcg atgaccggct ggcggctcgg ctacgggatc
     7321 atcccgagct ggatggtgcc cacctacagc cgtctcgtga tcaactcggt cagctgcacg
     7381 aacgcgtttg cccag
//
LOCUS       DASYGU010000147         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_950453, whole genome shotgun sequence.
ACCESSION   DASYGU010000147 DASYGU010000000
VERSION     DASYGU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950453"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGO32_03985"
     CDS             <1..399
                     /locus_tag="VGO32_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV7199943.1"
                     /translation="EANAIAMRLQGISAERPLTHDLLVSVLGAVHGNLSRIVVTHVSD
                     GTFHARLYLETAEGGETEIDSRTSDAIAVAIRTGSTIYVDERVLDEAGVEPESNGEVG
                     EEDEKLAVFRDFVNSLDVDLGPAEGRPPAE"
     gene            complement(405..1160)
                     /locus_tag="VGO32_03990"
     CDS             complement(405..1160)
                     /locus_tag="VGO32_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7199944.1"
                     /translation="MNGPTGSRPALTFFFPAYNEEANIERTVAIALEAIAPLASPLEV
                     LAIDDGSTDRTPELADALAAADPRVRVVHQPNRGYGGALKSGFANARGALVAFSDGDL
                     QFDLAEMSRLLDRLAYGSRPVDAVIGYRIKRRDPFQRLVIAKTYNLIVSVLFGLRVRD
                     IDCAMKVFRREVFDGLRLEAESPFLSAELLIKLNARGVKIGQVGVTHYPRMAGENTGA
                     SFRKILRTFRDIFKLRWKLWTDRERTLGSRPGA"
     gene            1188..>3040
                     /locus_tag="VGO32_03995"
     CDS             1188..>3040
                     /locus_tag="VGO32_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199945.1"
                     /translation="MPSASRRRFLDPFGTLLVLLVLGFALRVFIAYVLLPESGLRNDI
                     SSFAAWALRMAEVGPGGFYEPGVFADYPPVYMYVLWLVGEIAKFFEPILGISPIRPLI
                     KLPGMLADLGVAWVIYAISVRFLGEHPATRWLGSGARIGLIGAAFYLFNPGTLFNSAV
                     WGQMDAVGALVILASLYALGRGWTEAAGVGAVVAVLIKFQFGWLIPIVLVVGLKRHLF
                     GRSSDPALAARPDPVRILSSVAVSFGAAVLILLPFGMTLLPTGDPNTGILDKFLSATQ
                     TYQGLSINALNFWRNPWTGIWDVQQWGSDQTVVFTIGATSVTLAMVGVGLFAFGAVLA
                     LVAVARRDDMTGLLMGSLTMAIAFFGLPTRVHERYLFPAIALAAPLVGTAVRWAAAYV
                     ALSALFFLNIYWVYSADWTYLGPPPLAPGLNGDAFVRDPLLAATVFSQWGIYVLSFSS
                     LLLLGWVVRQAMKPAPVPGEAGADQDAVEAAAEAAAAAGAVRAARGRAGWRWLRQDPV
                     TEAERDPPRRLDRRDLVFFIVIVVVALVLRLWRLDLPRSMIFDEVYHARTAADFISDW
                     KWGWTRDPYEWTHPMLAKYLIAAGIEVANPNQVSGTIELDEPVTSLAVAPQR"
BASE COUNT          407 a         1054 c         1057 g          522 t
ORIGIN      
        1 gaggccaacg ccatcgcgat gcgcctccag ggcatcagcg ccgagcggcc gctgacccat
       61 gacctgctcg tgtcggtgct gggcgcggtc cacggcaacc tgagccggat cgtggtcacc
      121 catgtcagcg acgggacgtt ccacgcccgg ctgtacctcg agacggcgga gggtggcgag
      181 accgagatcg attcgcggac gtccgacgcg atcgcggtcg cgatccgcac cgggtccacg
      241 atttacgtcg acgagcgggt cctggacgag gccggcgtcg agccggagtc gaacggggag
      301 gtcggcgagg aggacgagaa gctcgccgtc ttccgcgact tcgtgaactc gctcgacgtg
      361 gacctcggtc ctgccgaagg acgcccgccg gccgagtagg ccggtcaggc cccgggtcgg
      421 ctcccgagcg tgcgctcccg atcggtccac agcttccacc gcagcttgaa gatgtctcgg
      481 aaggtgcgga ggatcttgcg gaagctggcg ccggtgttct cgccggccat tcgcgggtag
      541 tgggtcacgc ccacctggcc gatcttcacg ccccgtgcgt tcagcttgat gaggagctcg
      601 gctgacagaa acgggctctc ggcctcgagc cgaaggccat cgaacacctc gcgccggaag
      661 accttcatcg cacagtcgat gtcccgcacc cgcagcccga acaggaccga cacgatgagg
      721 ttgtaggtct tggcgatgac cagccgctgg aatgggtcac ggcgcttgat ccggtagccg
      781 atgaccgcgt ccacgggccg tgatccgtac gccagccggt ccaggaggcg cgacatctcg
      841 gccaggtcga actggaggtc gccgtcggag aaggcgacga gtgcgccgcg cgcgttggcg
      901 aacccggact tcagggcgcc cccgtatccg cggttgggct ggtggacgac ccggacccgc
      961 ggatcggcgg cggccaacgc atcggccagc tccggcgtcc ggtcggtgga tccgtcgtcg
     1021 atggcgagca cctcgagcgg cgaggcgagg ggtgcgatgg cctcgagggc gatcgccact
     1081 gtccgctcga tgttcgcttc ctcgttgtag gcgggaaaga agaaggtgag cgccggccgg
     1141 gagcccgtcg gaccgttcac aggggccacg atatactcac cgccgccttg ccgagcgcca
     1201 gccgccgacg attcctcgac ccgttcggca cgcttctcgt ccttctcgtc ctgggctttg
     1261 cgctgcgcgt cttcatcgcg tacgtcctgc tccccgaatc gggactccgc aacgacatct
     1321 cgtcgttcgc cgcctgggcg ctccgcatgg cggaggtcgg gcccggcgga ttctacgagc
     1381 cgggcgtctt cgccgactac ccgccggtgt acatgtacgt cctctggctg gtcggcgaga
     1441 tcgcaaagtt cttcgagccg atcctcggca tctctcccat tcgacccctg atcaagctgc
     1501 ccggcatgct cgccgacctg ggcgtcgcgt gggtcatcta cgccatcagc gtccggttcc
     1561 tgggtgagca tcccgccacc cggtggctcg gctcgggagc ccggatcggc ctcatcggag
     1621 ccgccttcta cctgttcaac ccgggcacgc tgttcaactc cgcggtctgg ggccagatgg
     1681 acgccgtcgg cgccctggtc atcctggcct cgctgtacgc gctgggacgg ggttggaccg
     1741 aggccgccgg ggttggggcc gtcgtcgcgg tcctgatcaa gttccagttc ggctggctga
     1801 tcccgatcgt gctggtggtg ggcctcaagc ggcacctgtt cggccgctcg tccgaccccg
     1861 cgctggccgc tcgaccggac ccggtccgga tcctcagctc ggtcgcggtc agcttcgggg
     1921 ccgcggttct catcctcctg ccgttcggga tgacgctcct gcccaccggc gacccgaaca
     1981 cgggaatcct ggacaagttc ctgtccgcca cccagaccta ccagggcctg tcgatcaacg
     2041 ccctgaactt ttggcgcaac ccgtggaccg ggatctggga cgtccagcag tggggcagcg
     2101 accagaccgt ggtgttcacc atcggcgcca ccagcgtcac gttggcgatg gtgggcgtcg
     2161 gcctgtttgc gttcggcgcg gtcctggccc tggtggcggt ggcccggcgg gacgacatga
     2221 ccgggctcct catgggctcg ctgaccatgg ccatcgcgtt cttcgggctg ccgacccgcg
     2281 tccatgagcg ctacctgttc ccggccatcg cgctcgccgc gcccctggtc gggaccgcgg
     2341 tccggtgggc cgcggcctac gtcgccctgt cggccctgtt cttcctgaac atctactggg
     2401 tctactcggc ggactggacg tacctcggtc cgcccccact ggccccgggc ctgaatggcg
     2461 acgcgttcgt ccgcgacccg ctgctggcgg ccacggtgtt cagccaatgg gggatctacg
     2521 tgctgtcgtt cagctcgctg ctgctcctgg gctgggtcgt ccgccaggcg atgaagcctg
     2581 ctccggtccc cggcgaggct ggggccgatc aggacgccgt cgaggcggcg gcggaggctg
     2641 cggctgccgc tggggccgtc cgggcggctc ggggccgtgc tgggtggcgc tggctgcgcc
     2701 aggacccggt caccgaggcc gagcgcgacc cgccgcgccg gctggaccgg cgggacctcg
     2761 tgttcttcat cgtgatcgtc gtggtggcgc tcgtcctgcg cctatggcgg ctggacctgc
     2821 cgcgctcgat gattttcgac gaggtctacc acgcccggac ggcggcggac ttcatctcgg
     2881 actggaagtg gggctggacc cgggacccct acgagtggac ccacccgatg ctggccaagt
     2941 acctcatcgc ggccgggatc gaggtggcga acccgaacca ggtgagcggc acgatcgagc
     3001 tggacgagcc cgtgaccagc ctggccgtgg cgccgcagcg
//
LOCUS       DASYGU010000148         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1104811, whole genome shotgun sequence.
ACCESSION   DASYGU010000148 DASYGU010000000
VERSION     DASYGU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104811"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VGO32_04000"
     CDS             <1..656
                     /locus_tag="VGO32_04000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7199946.1"
                     /translation="GFPTLQWYQAVFADEALLQAAVNSLIVGVLAATLATGLGFAVSV
                     ATIRFRYRGRVGLVGVAAMPLVVPFIVLGVALFMLFIFVDVPRSLLTIAIGHSIVALP
                     FALLILLARMIGLDPNLEDAAMDLGATYRVTLRRVVVPLMGASILSAWLTCFIVSFDE
                     VAIALFLAGGDPTFPVFLYGQLRFAGRLPVLIAMAVLLMLGTVTLSVIAFRIGRRNR"
     gene            complement(660..881)
                     /locus_tag="VGO32_04005"
     CDS             complement(660..881)
                     /locus_tag="VGO32_04005"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEV7199947.1"
                     /translation="MCRSIHLLRDGADVAPDDDITAAALQYVRKVSGYRKPSAANEAA
                     FNEAVAEVALASRHLLDHVASTHPAAHRH"
     gene            complement(904..1509)
                     /locus_tag="VGO32_04010"
     CDS             complement(904..1509)
                     /locus_tag="VGO32_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV7199948.1"
                     /translation="MDGLLLDTEIVWQGAEERLFAAHDKVFTREDKLAVIGTDFGFTA
                     RYFAERLGQPPEAGPALVEEMVMDMYDLLQEGVAGRPGAFELVARLRGRMPLALASNS
                     PRSLVDAALVSAQMADTFAVIVTSDDVEHSKPAPDIYLLVCERLGLAPADVLALEDSA
                     SGVASAKAAGLTCIAVPQFVETDVAAADRIIDSLEELLPEF"
     gene            complement(1554..>2280)
                     /locus_tag="VGO32_04015"
     CDS             complement(1554..>2280)
                     /locus_tag="VGO32_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7199949.1"
                     /translation="KATTALAQRLGKTAVEAADTPGFIVNRVARPFYLEALRILGAGG
                     ADAPTIDAALRGAGFRMGPFELIDTIGLDVNLAVSESVWEGFGRAPRYLPHALQQVLV
                     QAGRLGRKTNAGFYDYGADGQRGAAWSELAAAAEAPNATPLSEAAITDRVMAAIVNEA
                     ASAVEDQTARPDAIDTAMRLGTNWPRGPLAWGEERGLAQAVAILDALAADAPDARYAV
                     VPLLRDLAERDESFFTGTPPDPR"
BASE COUNT          313 a          795 c          796 g          376 t
ORIGIN      
        1 aggggtttcc gaccctgcag tggtaccagg ccgtgttcgc ggacgaggcg ctcctccagg
       61 cagcggtgaa ctcgctgatc gtgggcgtcc tggccgcaac cctggccact ggcctgggct
      121 tcgccgtcag cgtggcgacc atccggttcc ggtaccgcgg tcgcgtcggc ctggtcggtg
      181 tggccgccat gccgctcgtc gtcccgttca tcgtcctggg cgtcgcactc ttcatgctgt
      241 tcatcttcgt cgacgtcccg cgctcgttgc tgaccatcgc catcggtcat tccatcgtgg
      301 cgctgccgtt tgccctcctc atcctgctgg cccgcatgat cgggctggat ccgaatctcg
      361 aggacgcggc gatggatctc ggggccacct accgggtgac cctccgccgg gtcgtggtcc
      421 cgctcatggg cgcgtcgatc ctgtccgcat ggttgacctg cttcatcgtc tcgttcgacg
      481 aggtggcgat cgccctgttc ctggccggcg gggatccgac cttcccggta ttcctctacg
      541 gccagctgcg gtttgcgggc cgcctgccgg tgctcatcgc gatggccgtg ctgctcatgc
      601 tcgggacggt caccctgagc gtgatcgcct tccggatcgg ccgccgcaat cgctgaacgt
      661 cagtggcggt gagcagccgg atgggttgag gcgacgtggt cgaggaggtg gcgcgaggcg
      721 agggccacct cggccaccgc ttcgttgaac gcggcctcgt tcgcagccga gggcttccgg
      781 tagccgctca ccttgcgcac gtactggagc gcggcggcgg tgatgtcatc gtcgggcgct
      841 acgtcggcgc cgtcccggag aaggtggatg gatcgacaca tgcccacagc ctagcgcgcc
      901 gggtcagaac tcggggagga gctcctcgag cgagtcgatg atccggtccg cggcggcaac
      961 atcggtctcg acgaactgcg gcaccgcgat gcaggtcagt ccggccgcct tggccgatgc
     1021 gaccccggac gccgagtcct cgagagccag cacgtccgcg ggcgccaggc ccagccgctc
     1081 gcagacgagg aggtagatgt ccggcgccgg cttggaatgc tccacgtcgt cggaggtgac
     1141 gattaccgcg aacgtgtcgg ccatctgagc cgataccagc gcggcatcga ccagcgaccg
     1201 gggcgagttc gaggcgaggg ccagcggcat ccggccgcgg agcctggcga ccagctcgaa
     1261 cgcgccggga cgaccggcca cgccctcctg gagcaggtca tacatgtcca tcaccatctc
     1321 ctccacgagc gccggtcccg cctcgggagg ctgtccgagc cgctcggcga agtagcgagc
     1381 ggtgaacccg aagtcggtcc cgatcacggc gagcttgtcc tcgcgggtga agaccttgtc
     1441 gtgggccgcg aacagccgtt cctcggcccc ctgccagacg atctcggtgt cgagcaatag
     1501 gccgtccatg tcgaagacga ccgcttgata ccgatgcgga ggtgtagcgc ccgtcaccgg
     1561 ggatcaggcg gtgtgccggt gaagaacgat tcgtcacgct ccgccaggtc gcgaaggagc
     1621 ggcacgacgg cgtaccgggc atcgggcgcg tcggccgcca gcgcgtccag gatggccacc
     1681 gcctgcgcca ggccgcgctc ctccccccac gccagcggac cgcgtggcca gttggtcccg
     1741 agccgcatgg cggtgtcgat agcgtcgggt cgcgccgtct ggtcctcgac cgcggaggcg
     1801 gcctcgttga cgatggcggc catcacccgg tcggtgatcg cggcctcgga gagcggggtg
     1861 gcgttcggtg cctcggcggc ggccgcgagc tcggaccacg ccgccccacg ttggccatcg
     1921 gcgccgtagt cgtagaaccc cgcgttggtc ttgcgaccca gccgcccggc ctggacgagc
     1981 acctgctgga gggcatgggg cagatagcgc ggggcacggc cgaagccctc ccacaccgat
     2041 tcgctgaccg cgaggttgac gtccagcccg atggtgtcga tcagctcgaa cgggcccatc
     2101 cggaatcccg ccccgcggag cgcggcatca atcgtcggcg cgtcggcgcc acccgcgccc
     2161 aggatgcgca gcgcctcgag gtagaacggc cgcgccaccc ggttcacgat gaacccgggc
     2221 gtgtcggcgg cttcgaccgc ggtcttgccc aaccgctggg ccagggcagt tgtggccttc
//
LOCUS       DASYGU010000149        10117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_281350, whole genome shotgun sequence.
ACCESSION   DASYGU010000149 DASYGU010000000
VERSION     DASYGU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281350"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1004)
                     /locus_tag="VGO32_04020"
     CDS             complement(129..1004)
                     /locus_tag="VGO32_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEV7199950.1"
                     /translation="MISEGQDPGEGPGDYAAEIAAWVAERDRRLRDPNGWLALVGLHW
                     LSDGAQTFGADPSNAIHLVGRGVPERGGTLELTDGVVTLHPEPGSGPAVDGAPLDGDL
                     VLADDIGQPPTRVDIGALRVHVIRRGERIGLRVRDTELPALRSYAGVPRFELDPAWRV
                     TGHLERPPGLRTVDVPDVLGEVRAEESAGVVHFDAGGEHLHLDALPEDDDMLWLIFAD
                     TTNGAETYSGGRFLVTGPVAEDGSVVVDFNLAYNPPCVFSPYATCPLPWDSNRLPIAV
                     QAGERLPHVPHPAAD"
     gene            complement(1001..1591)
                     /locus_tag="VGO32_04025"
     CDS             complement(1001..1591)
                     /locus_tag="VGO32_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199951.1"
                     /translation="MPEISHVHLAVPDLTTLNRVIREGGAQSFPQWLSTDYGDRAQIE
                     WYGMRGALMLNVEPPDDPARKGLWPRPVDDWTEPPRGVILATVDAARAATDLAPVIGA
                     EAAWWETGEDPLLKATCRRTTLGRSVLVLAEPTGEGYVAACLARFGEGPVAVVLDGTT
                     RVGRAQDANPVSDGPARYVRLGAPDAPTLIFLPASA"
     gene            1623..2444
                     /locus_tag="VGO32_04030"
     CDS             1623..2444
                     /locus_tag="VGO32_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7199952.1"
                     /translation="MEKSGFIPVNGGRLYYEEAGDGPPLVMLHAGVANLRQWDAHVPV
                     FAEHYRVIRYDSRGYGASESEHVEFSNHGDLEQALDHLGVESTHLLGTSRSASIALNF
                     TVTNPQRVTSLIEVAGGGVGEPTDLSEAQVALGDAMDKASEEALEAKDWVRLADLETA
                     FWGDGPGQPGGRLPTPIRDQLHDWILTTYAAEKEEAIPQRLDPPADERVDQVTMPLLV
                     VIGSLDEAYTNATCRLLAETVPGARLEVFEGAAHMLNLEQPERFTRLILEFLASV"
     gene            complement(2454..3917)
                     /locus_tag="VGO32_04035"
     CDS             complement(2454..3917)
                     /locus_tag="VGO32_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV7199953.1"
                     /translation="MPGSTPDLPPLPASLSDRYQLEKPLAMGGSAEVWQGVDRVLKRP
                     VAVKLLHRHLLTDETMRARLTQEAQAAASLSHPGIVAIYDVAVDGDAAVIVMELVEGH
                     TLAEQLRRDQILPPRAAARIGAEVAEALEHAHQRGVIHRDVKAANVLIGPDGEARLVD
                     FGIARLLDDQAMQLTAPGTITGTLRSMAPEQLRGETAGPATDVYATGVLLTELLIGEP
                     PYVVTTPVELAEAQKTPPLVIGDAPPELSAIVRRALDPDISRRQSSAAGLAAELRSWL
                     ESDAVPAAAGTSDEMTEAAVVIPAAVASQPDPPEATKPEPEIPAPVPPGPSRSRDARL
                     WAVIGAAVLVALGVFVISQSLGPAGLGAASPSASVVPSLAETPAPTPVPTPIPTPVPA
                     PPTLEAAVQQFRDAVTAGQQNGEISEGAAEELVDEANRFVEDEDLRGGDINRIARDLN
                     RAIDEFEREGEIGSPTLATELRRLVDEMEAAARRQSG"
     gene            complement(3986..5482)
                     /locus_tag="VGO32_04040"
     CDS             complement(3986..5482)
                     /locus_tag="VGO32_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7199954.1"
                     /translation="MDHAVVIAGGGPTGLMLAGELALAGVDVVIIERRPNQDLPGSRA
                     GGLQSRTIEVLDQRGIADRFLGEGQVAQVAGFAMIRLDISDFPTRHPYGLGLWQNHIE
                     RILAGRVAELKVPIRYGSEVTGFAHDEIGVDVALSDGQSLRAEYLVGCDGGRSLVRKA
                     AGIEFPGWDPTTSSLIAQVEMTEEPETGIRRTPSGTHALGKVEYEVKGGDVIYAKGGT
                     VGVMLTESKVGNSDDPTLRDLSEGLIAVYGTDYGIHSPTFISRFTDMTRQAAAYRKGR
                     VLLAGDAAHVHSPVGGQGLNTGVQDAVNLGWKLAQVVNGISPDSLLDTYHDERHPIGA
                     RVLRTTMAQVALMRVDDRIEALRDSVSELLSMDEPRRRFAAMMSGLDIHYDLGEGHPL
                     LGRRMPDLDLITPDGPLRVFTLLHDARPVLVNIAEPGAFDITPWKNRVQLVDARYDGP
                     WELPALGAVAAPSAVVIRPDGYVAWVGDEAQLGLVEALTTWFGPPTAA"
     gene            5538..5978
                     /locus_tag="VGO32_04045"
     CDS             5538..5978
                     /locus_tag="VGO32_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HEV7199955.1"
                     /translation="MSPSESTVVPAERIIDYITTTFDGVDVVTAKEATFFSLDPVRHF
                     PSIATIVTTDDHDQASDLSRPGVYRLNIGLDRTTFRRLFPTDAKVEHDFSALDRLMPH
                     PVYGAQNWVCVLNPGEAIFENVVKPLLDEAYALEKARAKRRKGG"
     gene            complement(5980..7479)
                     /gene="gatA"
                     /locus_tag="VGO32_04050"
     CDS             complement(5980..7479)
                     /gene="gatA"
                     /locus_tag="VGO32_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV7199956.1"
                     /translation="MSELPLADRTISEIAPLLEAGELSSRDLVEACLDRIDRDAGRLN
                     TFLAVGAEQALEAADIADGARADGVRAPLLGIPYALKDIFVTRALNEDGQPDGGLPTT
                     AGSRILAGYLSPYASDAEDRMAAAGAILLGKTNCDEFAMGSSNENSAYGPVHNPWDET
                     LVPGGSSGGSSVAVASGQAFFATGTDTGGSIRQPAALSGVVGMKPTYGRVSRYGMVAF
                     ASSLDQCGPFGRSVRDVALVLGTLAGHDPRDSTCVDVPVPDYLAALDGEVRGLRLGVP
                     REFFVEGLEAGVETAVRAAIDTLAAAGADIVDVSLPSTDKGLATYYIITPAEASANLA
                     RYDGIRYGASAGVDDLLENYRLTRGAGFGLEVKRRIILGTYALSAGYYDAYYLKAQQV
                     RTLIKAEFDEVLSSVDALLAPTSPSVAFKIGAKTDDPLLMYLNDACTLPVNIAGLPGI
                     SVPCGLSDGLPVGFQVIGRAFDESTVLRVADAYERRAGFTELRPPTAHD"
     gene            complement(7486..7785)
                     /gene="gatC"
                     /locus_tag="VGO32_04055"
     CDS             complement(7486..7785)
                     /gene="gatC"
                     /locus_tag="VGO32_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV7199957.1"
                     /translation="MISRADVENVAALARLHLTDEELDLMEGQLSRILEAIAVLQDVD
                     TSMVGPTASVLPLENVMREDIPSDAGRLTRAAALGNAPLREGDHLRVPLVLKEGR"
     gene            7816..8685
                     /locus_tag="VGO32_04060"
     CDS             7816..8685
                     /locus_tag="VGO32_04060"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7199958.1"
                     /translation="MIEAPQVESLPVPGGRIERLVLRGGPGVPLLVVGGVETGLRLLA
                     GTEALLAQRWARRRATRTVIVVGRTIPEDPADADALLHPRVSAAAITVAITAPGAPYG
                     RVAIEAESGGGRISLWLTVDHPTIVSRLVLAASASETPTGSKMAPRMQEWIELGERGE
                     WGEFFRRMALQMRPGGEAGNAPPAGSFAAAASLQPVPATPERFLAELRGTLDPTSFVT
                     NRLKEIRVPTLLLAGGQDQVVPLPSSRLVAEHIPGARLEVDPECGHTVRSSFRGYDDL
                     VETFLVAGDLGSD"
     gene            complement(8682..9875)
                     /locus_tag="VGO32_04065"
     CDS             complement(8682..9875)
                     /locus_tag="VGO32_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199959.1"
                     /translation="MTAASPSRLLTPAFVLLAVATLAYFIADGMTLPATPLYVTDALG
                     GDDVAVGLTVGAFGLTALILRPLSGALADRRGRRPLLIGGSALFAIGMLGHLVAVSVP
                     LLIAMRLLLGAAEAFMFVAALTTASDLAPDDRRGEAINYLSLSLYVGIAIGPLIGEAL
                     LDVADFNAVWIAAAALAGLAVALAWRVPETRPPDLEPTDEPVPHRGLAARFLHPAAVG
                     PGLVLLAGTWGMGGFFAFLVLHARELGLDGAGSLFLLYAGIVILLRAAIPWLPDRLGY
                     ARTAALGLTAEFIGLLILGLAPGVASLYIGTAVFGFGATFMAPALLTMAVDRVGSTER
                     GAVLGSTSAFIDLGFGLAPAVLGSVAAAFGYGSVFAVSAFVALAGVGLLALMRRRAPA
                     SAIPA"
     gene            9903..>10117
                     /locus_tag="VGO32_04070"
     CDS             9903..>10117
                     /locus_tag="VGO32_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199960.1"
                     /translation="MSDPCTCPPDPSEARSRAPLSDEAAQIGEHFDERFQAWEPVGPE
                     ATDLLFSWLRDEGLSGSTIMDVGCGTG"
BASE COUNT         1565 a         3520 c         3601 g         1431 t
ORIGIN      
        1 ttcatctcgg ccagcatgtc gaagaagcgg cggatgcccg agggctggat gtcccgaacc
       61 gtgtcgttca ggaactcggc gggagcccgg acggtgccgg tcgcgtcggt ggtcatcggt
      121 ctggaagtct agtccgccgc cggatgcgga acgtggggca gccgctcccc ggcctgcacg
      181 gcgatgggca gccgattcga gtcccacggc agcgggcagg tcgcgtacgg gctgaacacg
      241 cacggcgggt tgtaggcgag gttgaagtcc accacgaccg atccgtcctc cgccacgggg
      301 ccggtcacca ggaagcggcc gccggagtag gtttccgccc cgttcgtcgt gtcggcgaag
      361 atcagccaca gcatgtcgtc atcctccggc agcgcgtcga ggtggaggtg ctccccgccc
      421 gcatcgaagt ggacgacacc ggccgactcc tccgcccgga cctcgcccag gacgtcgggc
      481 acgtccaccg ttcggaggcc cggcggccgc tcgaggtggc cggtcacccg ccaggcggga
      541 tccagctcaa atcgcgggac gccggcatac gaccgaaggg cgggcagctc ggtatcccgc
      601 acccgcagcc cgatgcgctc gccgcggcgg atgacgtgga cgcggagcgc accgatgtcc
      661 acccgggtcg gcggctggcc gatgtcatcg gccaggacca ggtcgccgtc cagcggcgcg
      721 ccatccaccg ccgggccgga cccaggttca ggatggaggg tcaccacgcc atcggtcagc
      781 tccagcgtcc cgccgcgctc gggcacgccg cggccgacga ggtggatcgc gttgctcgga
      841 tcggcgccga aagtctgagc gccatcgctc aaccagtgga gcccgacaag ggccagccag
      901 ccgttcgggt cgcggagccg ccggtcgcgc tcggcgaccc aggccgcgat ctcggccgcg
      961 tagtcgccag gcccctcccc ggggtcctgt ccctcactga tcacgcgctg gccggcagga
     1021 agatgagggt gggcgcgtcg ggtgcgccca ggcggacgta gcgggcgggg ccgtcgctca
     1081 ccgggttggc gtcctgggcc ctgcccactc gcgttgtccc gtccaggacg acggccaccg
     1141 gcccctcccc gaagcgggcc aggcaggcgg ccacgtaccc ctcgccagtg ggctcggcca
     1201 ggaccaggac actccgcccc agcgtcgtcc ggcggcaggt ggccttcagg agcgggtcct
     1261 cgcccgtctc ccaccacgcc gcctcggcgc cgatgaccgg cgcgaggtcg gtggccgccc
     1321 gtgcggcgtc gaccgtggcc aggatcacgc cccgcggcgg ttcggtccag tcgtccaccg
     1381 ggcggggcca gaggcccttg cgggcggggt cgtcgggggg ctcgacgttg agcatcagcg
     1441 cgccgcgcat cccgtaccac tcgatctggg cccggtcgcc gtaatcggtc gagagccatt
     1501 gcggaaacga ctgcgcgccg ccctcacgga taacgcggtt cagggtggtc agatcgggca
     1561 ccgcgaggtg gacgtggctg atctcgggca tcggtttcgc agtctagcgg taggatcggc
     1621 gcatggagaa gagcggattc atcccggtca acggcggtcg gctgtactac gaggaggccg
     1681 gtgacggccc gcccctggtc atgctccacg ccggtgtggc gaacctccgg cagtgggacg
     1741 cgcatgttcc cgtattcgcc gagcactacc gcgtgatccg atacgacagc cgcggctacg
     1801 gcgcctccga gagcgagcac gtcgagttct cgaaccacgg tgacctggag caggcgctcg
     1861 accacctggg cgtggagtcg acccatctgc tgggcacctc gcgttcggcg tccatcgcgc
     1921 tgaacttcac ggtcacgaac ccgcagcggg tgaccagcct gatcgaggtc gccggcggcg
     1981 gggttggtga gccgactgat ctatccgagg cgcaggtcgc gctgggcgat gccatggaca
     2041 aggcgagcga ggaggcgctc gaggccaagg actgggttcg gctggccgat ctggagaccg
     2101 cgttctgggg cgacggcccg ggccagccgg gcggccgcct gccgacgccc atccgggacc
     2161 agctccacga ctggatcctg accacctacg ccgccgagaa ggaggaggcc atcccccagc
     2221 ggctcgaccc gccggccgac gagcgcgtcg accaagtgac gatgccgctg ctggtggtca
     2281 tcggttccct cgacgaggcc tataccaacg ccacctgccg cctcctggcc gagaccgtgc
     2341 ccggcgcccg gctcgaggtc ttcgagggcg cggcccacat gctcaacctc gagcagcccg
     2401 agcgcttcac caggctgatc ctggagttcc tcgcctcggt ctgacggtca acctcagccg
     2461 ctctggcggc gggcagcggc ctccatctcg tccaccagcc ggcgcagttc ggtcgccagt
     2521 gtgggggaac cgatctcgcc ctccctctca aactcgtcga ttgcgcgatt gaggtccctg
     2581 gcgatccggt tgatgtcgcc accgcgcagg tcctcgtcct ccacgaaccg gttggcctcg
     2641 tcaaccagct cctcggccgc cccctcgctg atctcgccat tctgctgacc tgcagtcacg
     2701 gcatcgcgga actgctgaac ggcggcctcc agggtcgggg gcgcgggaac cggcgtggga
     2761 atgggggtgg gcaccggagt gggggctggt gtctcggcca gagatgggac gacggaggcc
     2821 gatggagacg cggctccgag gccggccggg cctagggatt gggagatcac gaatacgccc
     2881 agcgcgacga ggaccgccgc gccgatgacg gcccagagcc tggcatcccg gctccgtgag
     2941 ggtccgggcg gtacaggtgc cgggatttcc ggttcgggct tggtggcctc cggcggatcc
     3001 ggctgagagg ctactgcggc ggggatcacg accgccgcct ccgtcatctc gtccgaggtc
     3061 ccggccgcgg ccggcacggc gtcggattcg agccacgacc gcagctcagc ggcgaggccg
     3121 gcggcagacg actggcgccg cgagatgtcg gggtcgagcg cccgacggac gatcgcggaa
     3181 agctcgggcg gcgcgtcgcc gatgaccagc ggcggcgtct tctgcgcctc cgccagctcg
     3241 accggcgtgg tcacgacgta tggcggctcc ccgatgagta gctcggtcag gaggacgccg
     3301 gtggcgtaga catcggtggc cggtccggcc gtttcgccgc gcagctgctc gggggccatg
     3361 gagcggaggg tgccggtgat ggtccccggc gctgtcagct gcatggcctg gtcgtccagg
     3421 agtcgggcga tcccgaagtc cacgagcctg gcctcgccat cggggccgat cagcacgttg
     3481 gccgccttca cgtcccggtg gatcacgccg cgctgatggg cgtgctcgag cgcttcggca
     3541 acctcggcgc cgatccgcgc cgccgcgcgt ggcggcagaa tctggtcgcg ccgaagctgt
     3601 tcggcgaggg tgtggccttc caccagctcc atcacgatga ccgccgcgtc gccatcgacc
     3661 gcgacgtcat agatcgcgac gatgcccggg tgggacagtg acgccgcggc ctgcgcctcc
     3721 tgcgtcaggc gggcgcgcat ggtctcatcg gtcaggaggt ggcggtggag gagcttcacg
     3781 gccacgggac gcttgaggac gcggtcgacc ccctgccaga cctcggcgct gccccccatc
     3841 gccagcggct tctcgagctg ataccgatca gaaagtgaag cgggcagcgg cggcaggtcg
     3901 ggcgtcgagc cgggcatgcc ggacatccta acggcgggcc tgcggggagt ggtggtgcac
     3961 ccgatgcggg tcactcaccg gcgcgctacg ccgcagtcgg cggtccgaac caggtggtca
     4021 acgcctcaac gagcccgagc tgcgcctcgt cgcctaccca cgcgacatat ccatcgggcc
     4081 gaatcaccac ggccgaggga gcggcgaccg ccccgagggc cggaagctcc cacggaccat
     4141 cgtagcgggc gtcgacgagc tgaacccgat tcttccaggg cgtgatgtcg aaggcgccgg
     4201 gctcagcgat gttgaccagc accggccggg catcgtgcag caaggtgaaa acccgcagcg
     4261 gaccgtcagg ggttatcaag tcgagatcgg gcatgcggcg tccgagcagc gggtggccct
     4321 cgccgagatc gtagtgaatg tcgaggccgg acatcatcgc ggcgaatcgt cggcgaggct
     4381 cgtccatgct caggagctcg gacacgctgt cgcgcagcgc ctcgatccga tcgtcgacgc
     4441 gcatgagcgc gacctgcgcc atcgtggtgc ggagcacgcg ggcaccgatg gggtgccgct
     4501 cgtcatggta ggtatccagc aggctgtccg gcgagatccc gttgaccacc tgcgccagct
     4561 tccatcccag attcacagca tcctgcacgc cggtgttgag accctgccca ccgaccgggg
     4621 aatgcacatg ggcggcgtcc ccggccagca ggacccgtcc cttgcggtag gcggcggcct
     4681 gccgggtcat atcggtaaac ctggagatga aggtggggct gtggatcccg tagtcggtgc
     4741 cgtacacggc gatgaggccc tcgctcagat cgcgcagggt ggggtcgtcc gagtttccga
     4801 ccttcgactc ggtcagcatg acgccaaccg tgccgccttt cgcgtagatc acatcgccgc
     4861 ccttgacctc gtactccacc ttgcccagag catgcgtgcc cgaaggagtg cgacgaatgc
     4921 ccgtttccgg ctcctcggtc atctcgacct gggcgatcag gctgctcgtc gtcggatccc
     4981 agccggggaa ctcgatgccg gccgctttgc ggaccaggct gcgccctccg tcgcagccga
     5041 cgagatactc cgcccgtagc gactgaccat cggacagcgc gacgtcgacg ccgatttcgt
     5101 catgcgcgaa tcccgttacc tcagagccgt agcggatcgg cactttcagc tcggcgaccc
     5161 gaccagcgag gatgcgctcg atatggttct gccacagccc gaggccgtag ggatgccggg
     5221 tgggaaagtc gctgatgtcc aggcggatca tggcgaaccc ggcaacctgg gccacctgcc
     5281 cctcccccag gaaccgatcg gcgatgccgc gctgatcgag gacctcaatc gtgcgcgact
     5341 gcaaaccgcc tgcgcgcgag cccgggaggt cctggttggg gcgccgctcg atgatgacga
     5401 cgtcgacgcc ggccaaagcc agctcgcccg ccagcatcag ccccgtcgga ccgcctccgg
     5461 cgatcaccac cgcgtggtcc atcggctgat catggcgcgc ccgtggggtc ttgacatgca
     5521 tgttctagcc tctggtcatg agcccaagcg agagcacagt cgtccccgcc gaacggatca
     5581 tcgactacat cacgaccacc tttgacggcg tggacgtggt cacggccaag gaggccacgt
     5641 tcttctccct cgacccggtc cgacactttc cgagcatcgc gaccatcgtc accaccgacg
     5701 accacgacca ggcatcagac ctgtcgcggc ccggtgtcta tcgtctgaac atcggtctgg
     5761 atcgcaccac atttcgccgc ctgttcccca ccgacgccaa ggtcgagcac gacttcagtg
     5821 ccctcgaccg cctgatgccc catccggtct acggagcgca gaattgggtc tgcgtcctga
     5881 acccgggcga ggcgatattc gagaacgtcg tgaagccgct cctagatgag gcctacgcgc
     5941 tcgagaaggc gcgcgccaag cggcgcaagg gcggctaggt cagtcgtgcg cggtcggcgg
     6001 gcggagctca gtgaatccgg cgcggcgctc gtaggcatcg gccacccgca ggacggtcga
     6061 ttcgtcgaag gcccggccga tgacctggaa gcccaccggc agcccatccg acagcccgca
     6121 cgggaccgat attcccggca ggccggcgat gttcaccggc agggtgcagg cgtcgttgag
     6181 gtacatgagc agcggatcgt cggtcttggc cccgatcttg aacgccaccg atggtgaggt
     6241 gggcgccagg agcgcgtcca ccgaggagag cacctcatcg aactcggcct tgatcagggt
     6301 ccggacctgc tgggccttga ggtagtaggc gtcgtagtaa ccagctgata aggcgtacgt
     6361 gcccaggatg atccgccgct tgacctccag cccgaacccg gcgccgcggg tcaggcggta
     6421 attctccagt aagtcatcca cgccggccga ggcgccgtag cggatcccgt cgtagcgggc
     6481 caggttcgcg ctcgcctcgg ccggcgtgat gatgtagtag gtcgcgagtc ccttgtcggt
     6541 ggagggaagg gacacgtcaa cgatgtccgc gccggccgcg gcgagggtgt cgatcgcggc
     6601 gcggactgcg gtctcgaccc cggcctcgag gccctccacg aaaaactcgc gtgggacgcc
     6661 caggcgcagg ccgcgcacct ccccgtcgag cgcggccagg tagtcgggga ccggcacgtc
     6721 cacgcacgtc gagtcgcgcg ggtcgtgccc ggccagcgtt ccgaggacga gggcgacgtc
     6781 ccgcaccgaa cgaccgaatg gcccgcactg gtccagcgac gaggcaaagg ccaccatccc
     6841 gtatcggctc acccgaccgt aggtgggctt catcccgacc acaccggaga gggcggccgg
     6901 ctgacggatg gagcccccgg tgtccgtgcc ggtggcgaag aacgcctggc ccgaggcaac
     6961 cgccacgctg gaaccgccac tcgacccgcc cgggaccagc gtctcgtccc acgggttgtg
     7021 caccgggccg taggcgctgt tctcgttcga ggagcccatc gcgaactcgt cgcagttcgt
     7081 cttgcccagc aggatcgcgc cggcagcggc catccgatcc tcggcgtcac tcgcgtacgg
     7141 ggacaggtag ccggccagga tccgcgaccc ggcggtagtc ggcagtcccc catcgggctg
     7201 gccatcctcg ttcagggcgc gggtcacgaa gatgtccttc agggcgtacg ggatcccgag
     7261 cagcggggcc ctcacgccat cggcccgcgc gccgtcagcg atgtcggcgg cttcgagcgc
     7321 ctgctcggca ccgaccgcca ggaaggtgtt cagccggccg gcgtcacggt cgatccggtc
     7381 caggcacgcc tcgaccaggt cgcggctgga cagctccccg gcttcgagca acggggcgat
     7441 ctcgctgatg gtgcgatcgg cgagcggaag ctcggacatc ggtcgctagc ggccttcctt
     7501 gagcacgagc ggaacccgga gatggtcgcc ttcgcgcagc ggcgcattgc cgagggccgc
     7561 cgcgcgggtc aggcggccgg cgtcggacgg gatgtcctcg cgcatgacat tctcgagggg
     7621 cagcacgctg gcggtggggc cgaccatgct cgtgtccacg tcctggagca ccgcgatggc
     7681 ctccaggatc cgcgacagct ggccctccat gaggtccagc tcctcatcgg tcaggtggag
     7741 acgagccagc gcggcgacat tctcgacatc cgcgcgactg atcatgagcc gatgctagca
     7801 ggcgagaatg gaccgatgat cgaggcaccc caggtcgagt cgctgccggt ccccggcggc
     7861 cggatcgagc gcctcgtgct acgcggcgga cccggcgttc cgctgctggt ggtgggcggc
     7921 gtggagaccg gcctgcggct gctggcaggg accgaggcgc tccttgccca acgctgggca
     7981 cggcggcgcg cgacccgcac cgtgatcgtg gtcgggcgca cgatccccga ggaccccgcc
     8041 gatgccgatg ccctgctcca tccgcgggtc agcgccgcgg cgatcacggt cgcgatcacc
     8101 gcgccgggcg ccccgtacgg acgggtcgca attgaggccg aatcgggcgg cgggcggatc
     8161 tcgctgtggc tcaccgtgga ccacccgacg atcgtgtcgc ggctggtgct ggccgcgtcc
     8221 gcgtccgaga ccccgaccgg ttcgaagatg gcgccgcgga tgcaggagtg gatcgagctg
     8281 ggggagcgcg gcgagtgggg cgagttcttc cgccggatgg cgctccagat gcggccggga
     8341 ggcgaggccg gcaacgcgcc gcccgccggg tcgttcgcgg ccgctgcgag cctccagccg
     8401 gtgcctgcca ccccagagcg gttcctggcc gagctgcgcg ggacgctgga cccgacctcg
     8461 ttcgtgacca accggctgaa ggagatccgg gtgccgaccc tcctgctggc cggtggccag
     8521 gaccaggtcg tgcccctgcc gtcgtcccgc ctggtggcgg agcacatccc cggggcgcgg
     8581 ctggaggtgg accccgagtg cggccacacg gtccggtcct cgttccgtgg ctacgacgac
     8641 ctggtcgaga cgttcctggt cgcgggcgac ctcgggagcg actaggccgg gatcgcggac
     8701 gccggcgccc gccgccgcat gagcgccagc agcccgacgc cggccagggc cacgaacgcc
     8761 gacaccgcga acacggaccc gtagccgaac gcggcggcca ccgagccgag gacggctggg
     8821 gcgagcccga agccgaggtc gatgaaggcc gaggtcgagc ccaggaccgc gccacgctcg
     8881 gtggagccga cgcgatcgac ggccatggtc agcagcgcgg gcgccatgaa cgtggccccg
     8941 aatccgaaca ccgcggtacc gatgtacagg ctggccaccc ccggcgccag gcccaggatc
     9001 aggaggccga tgaactctgc ggtcagcccc aaggcggcgg tgcgggcgta tccgagccga
     9061 tcgggcagcc acgggatggc cgcccggagc aggatcacga tccccgcgta caggaggaac
     9121 aggctgccgg cgccatcgag cccaagctcg cgggcgtgga gcacgaggaa ggcaaagaac
     9181 ccgcccatcc cccacgtgcc ggcgaggagg acaaggccgg ggccaacggc cgccggatgg
     9241 aggaaccggg ccgcgagccc gcgatgtggg accggctcat cggtcggttc gaggtccgga
     9301 gggcgcgtct cgggcacccg ccaggcgagt gcgacggcca ggccggccag ggcggcggcc
     9361 gcgatccaga ccgcgttgaa gtccgcgacg tcgagcagtg cctcgccgat cagcggaccg
     9421 atcgcgatcc ccacgtacag cgacagcgaa aggtagttga tcgcctcccc acgacggtcg
     9481 tcgggggcca ggtcactcgc ggtggtcagc gcggccacga acatgaacgc ctcggcggcg
     9541 ccgagcagca gccgcatcgc gatgagcagg ggcacgctga ccgcgaccag atggccgagc
     9601 atgccgatcg cgaacagcgc cgagccaccg atcagcagcg gccggcgtcc gcggcggtcg
     9661 gccagggcgc cgctcagcgg tcggaggatg agcgcggtca gcccgaacgc gccgacggtc
     9721 agcccgaccg cgacgtcgtc gccgcccagc gcatcggtca cgtacagcgg agttgccggc
     9781 agggtcatcc catcggcgat gaagtacgcc agggtggcga cggcgaggag tacgaatgcg
     9841 ggcgtgagca ggcgagacgg ggacgcggcg gtcatgagtc cgggcagtct agactccgac
     9901 cgatgtccga tccctgtacc tgcccgcccg acccgtccga ggcccgttcc cgggcgccgc
     9961 tgagcgatga ggcggcgcag atcggtgagc atttcgacga gcgattccag gcctgggagc
    10021 ccgtcggtcc ggaggcgacg gacctcctgt tctcgtggct ccgtgacgaa ggcctgtccg
    10081 ggtccacgat catggacgtg ggctgcggca ccggtga
//
LOCUS       DASYGU010000150         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_744974, whole genome shotgun sequence.
ACCESSION   DASYGU010000150 DASYGU010000000
VERSION     DASYGU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744974"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VGO32_04075"
     CDS             complement(<1..2007)
                     /locus_tag="VGO32_04075"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEV7199961.1"
                     /translation="MATVARHRAVPAVAARPTAAIGEVRYLNRELSTLQFNERVLALA
                     EDPAAPLLERAKFAAIFAGNMDEFYQVRVAGLKRQVAAGTVSTSPDGLTAAQQLAKID
                     RRVRKLATRHARLFAEDILPGLAAAGVPIVRWAELDDEARAELGETFTDQIFPVLTPL
                     AVDPGHPFPYISNLSLNLAVLVRDQASRRRLFARIKVPPLLPRFMYAAGNGHGPAWVP
                     LEDVIAAHLDSLFPGMVVIESWAFRVTRHSDLEIDDDEAEDLLETIEEELRRQRFSRA
                     VRLEVEAGMPDHVIALLSRELQIGSGDTHELEGPLALADLWSLVALDRPDLKPAPFHP
                     VTPQVLVPTGEGPADIFAALRAGDVLVHHPYDSFAGSVQAFIEQAAVDPSVLAIKQTL
                     YRTSGESPIVDALIRAAAAGKQVVVLVELKARLDEEANIAWARKLEQAGCHVVYGLIG
                     LKTHCKVCLVVRQEGSLVRRYVHIGTGNYHPSTARNYEDLGLLTTDERLTADVTHLFN
                     VLTGYSRRSEYDALIVAPLNLRRRMLEMIEREASRSTTKRPGRISMKMNALVDVGIVE
                     ALYAASQRGVEVDLLVRGPCVLRPGVPGLSDTIRVRSIVGRFLEHSRIYRFGMGQDSE
                     FWIGSADMMDRNLDRRFEALVRVDEPALQERLDGILDLAWADTTN"
     gene            complement(2116..2667)
                     /locus_tag="VGO32_04080"
     CDS             complement(2116..2667)
                     /locus_tag="VGO32_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199962.1"
                     /translation="MSTSTRFRLLLPIVTASALLLATAGGVLGKCEHDPDACEGMSAT
                     LDPGGTLLAGGSEVVGVWVLEDEQPFAATSVELIFNRVADATTIRATASPVGPDGRWE
                     AAVTLPAGGTWTTAAVVQAPGYAGTFSLDTVAVGQLAAAASSPPAAAVIPPVLPIALI
                     AAVALGALAILRLGRRDATPARG"
BASE COUNT          476 a         1007 c         1094 g          453 t
ORIGIN      
        1 gttggtcgtg tcggcccacg ccaggtccag gatgccgtcg agccgctcct ggagggccgg
       61 ctcgtcaacc cggacgaggg cctcgaaccg ccgatcgagg ttccgatcca tcatgtccgc
      121 gcttccgatc cagaactccg agtcctggcc catcccaaac cgatagatcc gcgagtgctc
      181 caggaagcgg ccgacgatcg agcgaactcg gatcgtgtcg gacaggcccg ggacacccgg
      241 ccgcaggaca cagggtccac ggaccaggag atcgacctca accccgcgct gggaggccgc
      301 gtacagggcc tcgacgatgc ccacgtcgac cagggcgttc atcttcatcg agatgcgccc
      361 gggccgtttg gtcgtggacc gcgaggcctc ccgctcgatc atctcgagca tccgccgccg
      421 gaggttcagg ggcgcaacga tgagcgcgtc gtactccgac cgccgcgagt agccggtcag
      481 gacattgaac aggtgggtga cgtcggccgt caggcgctcg tcggtggtga gcaggccgag
      541 gtcctcgtag ttccgtgcgg tggagggatg gtagttgccg gtcccgatat ggacgtagcg
      601 ccggacgagg ctgccttcct ggcggacgac gaggcagacc ttgcagtggg tcttcagacc
      661 gatgagcccg tacacgacgt ggcagcctgc ctgctccagc ttccgggccc acgcgatgtt
      721 cgcctcctcg tccagccggg ccttcagctc gaccaggacc accacctgtt tgccggcagc
      781 ggcagcccgg atcagggcgt ccacgatcgg gctctcgccc gaggtgcggt acagcgtctg
      841 cttgatggcc agtaccgagg ggtcgacggc cgcctgctcg atgaaggcct ggacgctgcc
      901 cgcgaacgag tcgtacgggt gatggacgag gacgtcgccg gcgcgcaacg cggcgaagat
      961 gtccgccggc ccctcgccgg tcggaaccag gacctggggc gtgaccgggt ggaacggtgc
     1021 cggcttgagg tccgggcgat ccagcgccac caggctccac aggtcggcga gcgcgagcgg
     1081 gccctcgagc tcgtgggtgt ctcccgagcc gatctggagc tcgcggctga ggagcgcaat
     1141 gacatggtcc ggcattcccg cctcgacctc cagccgcacg gcgcgcgaga accgctggcg
     1201 gcgaagctcc tcctcgatcg tctcgagcag gtcctccgcc tcgtcgtcgt cgatctcgag
     1261 gtccgagtgg cgggtcacgc ggaacgccca cgactcgatc accaccatgc ccgggaagag
     1321 gctgtccagg tgagccgcga tcacatcctc gagcggaacc caggccggtc cgtgtccgtt
     1381 gcccgcggcg tacatgaacc gcgggaggag aggcgggacc ttgattcggg caaacagccg
     1441 tcgccggctc gcctggtcgc gaacgaggac ggccaggttg agcgacaggt tggagatgta
     1501 cgggaacggg tgaccgggat ccacggcgag cggcgtgagg accgggaaga tctgatcggt
     1561 gaatgtctcg cccagctccg cgcgggcctc gtcgtcaagc tcggcccagc gaacgatcgg
     1621 gacgccggcc gcggcgagtc cgggcaggat gtcctccgcg aagaggcggg cgtgccgcgt
     1681 cgccaacttt cggacccgcc gatcgatctt tgccagctgc tgggcggcgg tcagtccgtc
     1741 gggagaggtc gaaaccgtgc ccgcagccac ctgccgcttc aggccggcga cccggacctg
     1801 gtagaactcg tccatgttgc cggcgaagat ggcggcgaac tttgcgcgct ccaggagggg
     1861 cgcagccgga tcctcggcaa gggccagcac ccgttcattg aactgaaggg tcgagagctc
     1921 ccgattgaga tagcggactt cgccgatcgc ggcggtgggg cgcgccgcga ccgccggaac
     1981 cgcccggtgt cgcgcgacgg tggccattgg gctacgtagc gtacgaggcg gctgataacg
     2041 ggtcaataaa cagcggtccg gggactatcg atccaccgga ccgctgggtc gcggagcggg
     2101 tcgtgcccgc gacggctatc cccgggcggg agtcgcatcc cggcgtccga gcctgaggat
     2161 ggcgagtgcg ccgagcgcca ccgccgcgat cagggcgatg ggcagcaccg gcgggatcac
     2221 ggcggcggcc ggcgggctgg acgcggcggc ggcaagttgg cccaccgcga ccgtgtccag
     2281 gctgaacgtg ccggcatagc ccggagcctg gacgaccgcg gccgtggtcc atgtccctcc
     2341 ggccggaagc gtgacagcgg cctcccaccg accgtccggt ccgacgggcg aggcggtcgc
     2401 tcggatggtg gtcgcgtccg ccacccggtt gaagatgagc tccacgctgg tggcggcaaa
     2461 cggctgctcg tcctccagca cccagacgcc gaccacttcc gatccgccgg ccagcagggt
     2521 tccgcccgga tccagggtgg cactcatgcc ctcgcacgcg tccgggtcgt gctcgcactt
     2581 ccccagcacg ccgccggcag tggccagcag gagcgcggac gcggtaacga tggggagtag
     2641 gagccggaat cgggtcgatg tcgacatcgg gatacctcgc ggacgcggct tggttgacgt
     2701 gatccctaca ccgtggtgat ggcatcggtt ccggtcaggg ccggttccgg ctcactttca
     2761 tactggatcc gtgctgacgc gcgggaacca ggtggcgatg gccggtgtat cgatcctgcc
     2821 gggtggaggg ctgtccactg tccgaccgtg aactgatgga acgggcgcga cgcggtgacg
     2881 tcgcggccta cgaggagctc gtgcgccgat acgaggaggt cgcctaccgg accgcctacc
     2941 tggtctgcgg cgatgccgat gatgcccgcg acgccgccca ggaagggttc gtccgcgcgt
     3001 ggcgtgcgct gcccagtttc cggagcgggg
//
LOCUS       DASYGU010000151         7718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1567936, whole genome shotgun sequence.
ACCESSION   DASYGU010000151 DASYGU010000000
VERSION     DASYGU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567936"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /gene="rplD"
                     /locus_tag="VGO32_04085"
     CDS             complement(<1..335)
                     /gene="rplD"
                     /locus_tag="VGO32_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV7199963.1"
                     /translation="MATTPVLTATGTKKGSVVLADSLFAAPVRTPLIHQVAMAQLAGR
                     RVGTADTLTRGEVRGGGKKPWRQKGTGRARQGSKNSPQWRGGGVVFGPHPRGYEVKVN
                     AKVRRAALRG"
     gene            complement(335..961)
                     /gene="rplC"
                     /locus_tag="VGO32_04090"
     CDS             complement(335..961)
                     /gene="rplC"
                     /locus_tag="VGO32_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV7199964.1"
                     /translation="MTVGLIGRKLGMTRLFAEDGSVVPVSVISAAPNLVTRIRSVERD
                     GYAAVQVGAGSARRLTKPVAGQLPKMDKDAPKPAVMREFRVDSTDGYEPGQALDVTLF
                     EAGDLVDVTGTSKGRGFAGTVKRHHFHRGPVTHGSTNTRQPGSIGAGTTPGRVFKNTK
                     MSGHMGDERITVQKLQVFRVDAERQLLLVRGAVPGARNGLVVIRKASA"
     gene            complement(969..1277)
                     /gene="rpsJ"
                     /locus_tag="VGO32_04095"
     CDS             complement(969..1277)
                     /gene="rpsJ"
                     /locus_tag="VGO32_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV7199965.1"
                     /translation="MAKQRIRIRLRAYDHKLLDQSAEQIVETAQRTGAEIVGPVPLPT
                     RIEKFTVIRGPFIHKDGREQFEIRTHKRLIDIIDPSSKTVDALMRLSLAAGVDIEIKM
                     "
     gene            complement(1286..2488)
                     /gene="tuf"
                     /locus_tag="VGO32_04100"
     CDS             complement(1286..2488)
                     /gene="tuf"
                     /locus_tag="VGO32_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV7199966.1"
                     /translation="MAKQKFDRTKPHINVGTIGHVDHGKTTLTAAITKYLALKGGASF
                     RAFDSIDNAPEERARGITIAIAHVEYQTDARHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVEVPSIVVFLNKVDAVDDEMLIDLVEMEVRELLSK
                     QQFPGDDIPVIRGSALKALESTNEDPEADEYKPIKELMDAVDSYIPTPIRETDKPFLM
                     PVEDVFGIKGRGTVATGRIERGMVKTGETIEQVGVHAKAKQLVVTGVEMFNKTLDEGQ
                     AGDNVGLLLRGIEREEIERGQVLAKPGSIKPGTKFKAEVYVLSKEEGGRHTPFYTGYR
                     PQFYLRTTDVTGAIELPAGVEMIMPGDNVNIDVELITPIAIEQGQRFAIREGGHTVGA
                     GVITALTA"
     gene            complement(2517..4592)
                     /gene="fusA"
                     /locus_tag="VGO32_04105"
     CDS             complement(2517..4592)
                     /gene="fusA"
                     /locus_tag="VGO32_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV7199967.1"
                     /translation="MATEYPLERTRNIGIIAHIDAGKTTVSERILFYTKKIYKIGEVH
                     EGAATMDWMEQEQERGITITAAATTCFWADHRINLIDTPGHVDFTVEVERSLRVLDGA
                     VVVFDGVAGVEPQSETVWRQADRYGVPRFCFVNKLDRTGADYWRVVDMIRDRLGAVAV
                     PVQIPIGTEAAFRGVIDLVEMKAITYGADADDPGQTGPIPAEMAEEAGQWHDKMVEAL
                     ADLDDDVAHKFLEGQPVEPDQLRAVLRTGTLAYQIVPVLCGSALKNKGIQPMLDAVIE
                     YLPSPLDVPPVTGTDPRTHEQVTRHTDPSEPFAALAFKIAADPFVGKLAFFRVYSGTL
                     KSGSYVYNSTKDVRERIGRLVQLHANHREEVESVSAGDIAAAVGLKSTFTGDTLTDEA
                     HPIVLEAITFPEPVIELAVEPKTKADQDKMAIALQRLAEEDPTFRVSTDQESSQTRIA
                     GMGELHLEVIVDRMLREFKVQANVGRPQVSYREAIRKAAKAEGRLVRQTGGKGQYGHA
                     VITLEPLERGSGYVFESKIVGGAIPREFWKAVEEGIRDALAGGIVAGYPLIDAKATLI
                     DGSFHEVDSSEMAFKIAGSMAAKAAVAKADPTILEPVMKVEVVTPEDFMGDVIGNLNA
                     RRGSVDGIEERATSRVIGAHVPLAEMFGYATDLRSMTQGRATYSMEFSRYSEVPQSIA
                     NELIAKSKS"
     gene            complement(4625..5098)
                     /gene="rpsG"
                     /locus_tag="VGO32_04110"
     CDS             complement(4625..5098)
                     /gene="rpsG"
                     /locus_tag="VGO32_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV7199968.1"
                     /translation="MPRRATVARKSKYDETLSGTALTMDQFTNKLMHGGKRQLASRIL
                     QDALARAESQAGRSGIEVLEGAMRNAMPLIEVKPKRVGGSTYQIPVEVRADRRVSLGM
                     RWLIEAARKRNGRSMSEKLAAELIDASNGLGAAVKRREDTHRMAEANKAFSHFRW"
     gene            complement(5103..5522)
                     /gene="rpsL"
                     /locus_tag="VGO32_04115"
     CDS             complement(5103..5522)
                     /gene="rpsL"
                     /locus_tag="VGO32_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV7199969.1"
                     /translation="MPTINQLVRKGRKPVRKKVKAPALRRTFNNLTQTASEGRGAPQR
                     RGVCLQVMTRTPKKPNSALRKVARVRLTNGMEVTAYIPGIGHNLQEHSVVLVRGGRVP
                     DLPAVKYHIIRGPLDAAGVRDRRQGRSKYGAKSAGKA"
     gene            5853..7403
                     /locus_tag="VGO32_04120"
     CDS             5853..7403
                     /locus_tag="VGO32_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7199970.1"
                     /translation="MRVGVFLAMAMFVLVVDTSLMNVSISAVVQDLGTTVSGVQSAIA
                     LEALVSAAFILISSKVGDLIGRRLAFILGLIGYAIGATAMVLTQTLGPVIFFWAILGG
                     LGASLLLPAMQSLIHGNFTGAAQRRVYAMVGASAAIAAAVGPLVGGFITTFLSWRVGF
                     ALEVVVIVVVLAGMRLVRDAPYTGERGIDVVGAGLSVVGMGGIVLGILVWQEGGEAVA
                     AFLVAGAVSLALFAWWLVRRKRQGKLTLLDPDLFRSKHFRLGVSEQGLQQIALGGTMI
                     ALPIYLQMVLEYNALESGLSIAPLSLSMFAVALLAGRRAGNTRPSLIIRGGFALLALG
                     AAILLPIVPRADSGWWLAIPLLIAGSGLGLLVSQLNNYTLSPISEERISEAAGVNSAA
                     GSFGLSFGLAMAGAVLMGSLALTFTNMAAQSEVLTPDEQQQVAAALEEDAQIMSNTLL
                     EEQLAGQPEDVQAEIIRINTEARPLALQFALLVPLIAALLGLVNSFRMVRLGDIKPKA
                     NLEGVSLG"
     gene            complement(7416..>7718)
                     /locus_tag="VGO32_04125"
     CDS             complement(7416..>7718)
                     /locus_tag="VGO32_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199971.1"
                     /translation="LAPDAEVVDVVAGVENPIPVSVTGYLLSGDGATLTVQFWGGVPE
                     CYGVANASVDTNAQPWVVSISEGHVPGAEVCIEIALAKAFVFRLDTQLLRDGSLTG"
BASE COUNT         1082 a         2594 c         2627 g         1415 t
ORIGIN      
        1 ccgcgcagcg cggcccgacg caccttggcg ttgaccttga cctcgtagcc gcgcgggtgc
       61 ggcccgaaga ccacgccgcc gccgcgccac tggggcgagt tcttgctgcc ctggcgggca
      121 cgaccggtgc ccttctggcg ccatggcttc tttccgcccc cgcgcacctc gccgcgggtc
      181 agggtgtccg cggtgcccac ccggcgtcca gccagctggg ccatcgcgac ctggtggatg
      241 agcggcgtgc ggaccggagc ggcgaacagc gaatcagcca gcacgaccga gcccttcttc
      301 gtgcccgtgg cggtcaggac cggggtcgtg gccatcaggc gctcgccttc cggatcacga
      361 ccagcccatt ccgggcgccg gggaccgcgc cacggaccag cagcagctgc cgctcggcgt
      421 cgacgcggaa cacctggagt ttctggaccg tgatccgctc atcgcccatg tggccgctca
      481 tcttggtgtt cttgaagacg cgccccggcg tggtgccggc cccgatcgat cctggctggc
      541 gcgtgttcgt ggagccgtgg gtcaccggtc cgcggtggaa gtgatggcgc ttgacggtgc
      601 ccgcgaagcc gcggcccttg ctggtccccg tcacgtcaac caggtcaccc gcttcgaaca
      661 gggtcacgtc cagggcctgg cccggctcgt agccgtcggt cgagtccacg cgaaactcgc
      721 gcatgacggc cggcttgggc gcgtccttgt ccatcttcgg cagttggccg gcaacgggct
      781 tggtgagacg gcgggcggac ccggcgccga cctgcacggc ggcatagccg tcgcgctcga
      841 cgctccggat tcgggtgacc aggttcgggg cagcggaaat gacgctcacc gggacgacac
      901 tgccgtcctc ggcgaagaga cgcgtcatgc cgagcttgcg cccgatcaac ccaaccgtca
      961 ctccaccctc acatcttgat ctcgatgtcg acgcccgctg ccagcgagag ccgcatcagc
     1021 gcatccaccg tcttggacga cggatcgatg atgtcgatca gccgcttgtg cgtccgaatc
     1081 tcgaactgct cccgtccgtc cttgtggatg aacgggccgc ggatgacggt gaacttctcg
     1141 atccgcgtcg gcaacggaac cgggccgacg atctcggcgc cggttcgctg ggccgtctcc
     1201 acgatctgct cggccgactg gtcgagcagc ttgtggtcgt acgcccgaag ccgaatgcga
     1261 atgcgctgct tggccatgaa cctgcctacg cggtgagcgc cgtgatcacg cccgcaccga
     1321 ccgtgtggcc gccttcgcga atcgcgaagc gctgcccctg ctcgatggcg atgggcgtga
     1381 tcagctcgac gtcgatgttg acgttgtcac cgggcatgat catctcgacg ccggccggca
     1441 gctcgatggc gccggtgaca tcggtcgtcc gcagatagaa ctgcggccga tagccggtat
     1501 agaacggcgt gtggcgcccg ccctcctcct tggagaggac gtagacctcg gccttgaact
     1561 tcgtgcccgg cttgatcgat cccggcttgg ccaggacctg gccgcgctcg atctcctcgc
     1621 gctcgattcc gcggagcaga agccccacgt tgtcgccggc ctggccctcg tcgagggtct
     1681 tgttgaacat ctcgacaccg gtcacgacca gctgcttggc cttggcgtgg acgccgacct
     1741 gctcaatggt ctcgccggtc ttgaccatcc cgcgctcgat gcggccggtc gccaccgtgc
     1801 cgcgaccctt gatcccgaac acgtcctcga ccggcatgag gaacggcttg tccgtctcac
     1861 ggatcggggt cgggatatag gagtcgacgg cgtccatcag ctccttgatc ggcttgtatt
     1921 catccgcctc cgggtcctca ttcgtggact cgagggcctt cagggccgag ccgcggatga
     1981 ccgggatgtc atcccccggg aactgctgct tgctgaggag ctcgcggact tccatctcga
     2041 ccaggtcgat gagcatctca tcgtccaccg cgtccacctt gttcaggaac acgacgatgg
     2101 acgggacctc gacctggcgg gcgaggagga tgtgctcgcg ggtctggggc atcgggccgt
     2161 ccgcggccga cacgaccagg atcgcgccgt ccatctgggc ggcaccggtg atcatgttct
     2221 tgatgtagtc cgcgtggcca gggcagtcga cgtgcgcgta gtgccgcgca tcggtctggt
     2281 actcgacgtg ggcgatcgcg atcgtgatgc cgcgggctcg ctcttccggc gcgttgtcga
     2341 tcgagtcgaa cgcgcggaag ctggccccgc ccttgagcgc gaggtacttc gtgatggccg
     2401 cggtcagcgt ggtcttgccg tggtcgacgt gaccgatggt gcctacgttg atgtgcggct
     2461 tggtgcggtc gaacttctgc ttggccatgg tgctttttct tcgatcctcc tggtggtcag
     2521 ctcttggact tggcgatcag ctcgttggca atggactgcg ggacttcgct gtagcgcgag
     2581 aactccatcg agtaggtggc ccgaccctgg gtcatggacc gcaggtcggt ggcgtagccg
     2641 aacatctcgg ccagcgggac gtgggctccg atgacgcgcg aggtggcccg ctcctcgatc
     2701 ccgtccacgc ttccgcggcg ggcattcagg ttgccgatga cgtcccccat gaagtcctcg
     2761 ggggtgacga cctcgacctt catgaccggc tcgagaatcg tgggatcggc cttcgcgacg
     2821 gccgccttgg cggccatcga accggcgatc ttgaaggcca tctcggacga gtcgacctcg
     2881 tggaacgagc cgtcgatgag ggtggccttg gcgtcgatca ggggatagcc ggccacgatg
     2941 cccccggcga gcgcgtcgcg gatgccctcc tcgaccgcct tccagaactc gcgggggatg
     3001 gcgccgccca cgatcttgga ctcgaagacg tagccgctcc cccgctcgag tggctcgagc
     3061 gtgatcaccg cgtgtccgta ctggcccttg ccgccggtct gacggacgag gcggccctca
     3121 gccttggccg ccttccggat cgcctcgcgg tacgagacct gcgggcggcc gacgttggcc
     3181 tgcaccttga attcgcggag catgcggtca acgatgacct cgaggtggag ctcgcccatg
     3241 cccgcgatcc gggtctgcga gctctcctgg tcggtgctga cccggaaggt cgggtcctcc
     3301 tcggccaacc gctggagggc gatcgccatc ttgtcctggt cggccttggt cttgggctcc
     3361 acggccagct cgatgaccgg ctcggggaag gtgatcgcct cgaggacgat cgggtgggcc
     3421 tcgtcggtca gggtgtcacc ggtgaacgtg ctcttcaggc cgaccgcggc ggcgatgtca
     3481 ccggcgctga ccgattcgac ctcctcgcgg tggttggcat ggagctggac caggcgcccg
     3541 atccgctccc tgacgtcctt ggtcgagttg tagacgtacg acccgctctt cagcgtcccg
     3601 ctgtacaccc ggaagaacgc cagcttgccc acgaatgggt cggcggcgat cttgaaggcg
     3661 agggccgcga acggctcgct cgggtcggtg tggcgggtga cctgctcgtg ggtgcgggga
     3721 tcggtgccgg tgaccggggg cacgtcgagc gggcttggca ggtactcgat gaccgcgtcg
     3781 agcatgggct ggatgccctt gttcttcagc gccgacccgc acaggaccgg aacgatctga
     3841 taggcgagcg tgcccgtgcg cagcacggcg cgcagctgat caggctcgac cggctgtcct
     3901 tcgaggaact tgtgggcgac gtcgtcgtcc aggtcggcca gggcctcgac catcttgtcg
     3961 tgccattggc ccgcctcctc ggccatttcg gccggaatgg gaccggtctg gcctgggtcg
     4021 tccgcatcgg cgccgtaggt gatggccttc atctcgacca ggtcgatgac gccccggaac
     4081 gcggcctcgg tcccgatcgg gatctggacc gggacggcca ccgcgcccag gcggtcgcga
     4141 atcatgtcca ccacacgcca gtagtcggcg cccgtccgat cgagcttgtt cacgaaacag
     4201 aagcgcggaa cgccgtaccg atccgcctgc cgccaaacgg tctcggactg gggctcgaca
     4261 ccggccacgc cgtcgaacac gacgaccgcg ccgtcgagca cgcggaggct tcgctccacc
     4321 tcgacggtga aatccacgtg gccgggcgtg tcaataaggt tgatgcggtg atcggcccag
     4381 aagcaggtcg tggccgcggc ggtgatcgtg atcccgcgct cctgctcctg ctccatccag
     4441 tccatcgtgg ccgcgccctc gtggacctcc ccgatcttgt agatcttctt ggtgtagaag
     4501 aggatccgct cggacacggt ggtcttgccc gcgtcgatgt gggcgatgat cccgatgttc
     4561 cgggtccgct cgaggggata ctcggtcgcc atctcgctgc ccttggttcg cggggcggtc
     4621 gcgactacca ccggaagtgg ctgaaggcct tgttggcctc cgccattcgg tgcgtgtctt
     4681 cgcggcgctt gacggcggcg cccaggccgt tcgaggcgtc gatcagctcg gctgccagtt
     4741 tctcactcat ggatcggccg ttccgcttgc gggcggcttc gatgagccag cgcatgccaa
     4801 gagaaacgcg ccgatcggcg cgtacttcga cggggatctg gtaggtcgag ccgccgaccc
     4861 gctttggctt gacctcgatg agcggcatcg cgttgcgcat cgcgccctcg aggacctcga
     4921 tgccggagcg cccggcctgg gactccgcgc gagccaacgc atcctggagg atgcgcgagg
     4981 cgagctggcg cttgccgcca tgcatgagct tgttcgtgaa ctggtccatg gtcagcgccg
     5041 tgcccgacag ggtctcgtcg tacttggatt tgcgggcaac ggtggcgcgg cgcggcatgg
     5101 gactaggcct tccccgccga cttggcgccg tacttgctgc gcccctgccg gcgatcccgg
     5161 acgccggccg cgtcaagcgg accgcggatg atgtggtact tgaccgccgg caggtccggc
     5221 acccgaccgc cacggaccag gaccaccgag tgctcctgga ggttgtggcc gatgccgggg
     5281 atgtaggcgg tgacctccat cccgttcgtg agtcggacgc gggcgacctt ccgaagcgcg
     5341 gagttcggct tcttgggcgt tcgagtcatg acctggaggc agacaccgcg tcgctggggc
     5401 gcgccgcgcc cctcggaggc ggtctgggtc aggttgttga acgtccgccg caatgcgggc
     5461 gccttgacct tcttccgaac cggcttgcgg cccttgcgga ccagctggtt gatggtcggc
     5521 aaccgatacc tctggtgact ctgcagggat gatggtggac gggttccgca tcgctccgac
     5581 ggaccctggt gggggcacgg accgagaggg tcagaccctc ttggcggaac acacacgaac
     5641 ggcgtcccgt ggcgtgggac accgatctgg gagtataggt tcagcgcgga ttgagtgtca
     5701 attgtgggcc cccgggacca ccccgtgcag gtgggtatcc cgtaccggaa cacggtccgc
     5761 taggatcgcc cgacatggcc gaaaccgccg agaccgaagc ccctgatctg ccgcgcgagc
     5821 ccgcgatcgg cgccgaggaa ggctcaggcg acatgcgggt cggcgttttc ctggcgatgg
     5881 ccatgttcgt cctcgtcgtc gacacgtcgc tcatgaacgt gtccatctcg gccgtggtcc
     5941 aggacctggg cacgacggtg agcggcgtgc agtcggcgat cgcactcgag gcgctcgtct
     6001 cggcagcctt catcctgatc agcagcaagg tgggcgacct catcgggcgt cgtctggcct
     6061 tcatcctcgg cctgatcggc tacgccatcg gggccacggc catggtcctg acccagaccc
     6121 tgggcccggt catcttcttc tgggcgatcc tcggtggact cggtgcgtcg ctcctgctcc
     6181 ccgccatgca gtcactcatc catggcaact tcaccggcgc ggcccaacga cgggtctacg
     6241 ccatggtcgg tgcctcggcc gccatcgcgg ccgcggtcgg tcccctggtg ggtggtttca
     6301 tcacgacgtt cctctcgtgg cgggtggggt tcgccctgga ggtcgtggtc atcgtcgtcg
     6361 tcctggcggg catgcgtctc gtgcgcgatg ccccctacac cggggagcga gggatcgacg
     6421 tggtcggcgc cgggctgtcg gtggtcggaa tgggcgggat cgtgctcggc attctcgtat
     6481 ggcaggaggg cggtgaggct gtcgccgcgt tcctggtcgc gggcgcggtc tcgctcgcgc
     6541 tcttcgcgtg gtggctcgtc cgccggaagc gccagggcaa gctgaccctc ctcgaccctg
     6601 acctgttccg ctcgaagcac ttccgactcg gcgtatccga gcagggtctc cagcagatcg
     6661 ccctgggcgg gacgatgatc gcgctcccga tctatctcca gatggttctc gagtacaacg
     6721 ccctcgagtc cggactctcg atcgcccctc tgtccctgag catgttcgcg gttgccctcc
     6781 tcgccggacg gcgggcgggg aacacccgtc cgagcctgat catccgaggc ggctttgccc
     6841 tgctcgcact cggggcggcc atcctcctcc ccatcgtccc gcgcgcggat tcgggatggt
     6901 ggctcgcgat tccgctcctg atcgccgggt ccggtctcgg gctgctggtg tcccagttga
     6961 acaactacac gctttccccc atctccgagg aacggatcag cgaggccgcg ggggtgaact
     7021 ctgcggcagg ctcattcggg ctttcgttcg ggttggcgat ggctggcgcc gtcctgatgg
     7081 gcagcctggc cctgaccttc accaacatgg ccgcccagag cgaggtgctc acgccggacg
     7141 agcagcagca ggtcgccgcc gccctggaag aggacgcgca gatcatgagc aatacgctgc
     7201 tcgaggagca gctcgccggt cagccggagg atgtccaggc cgagatcatc cggatcaaca
     7261 ccgaggcgcg gccgctggcc ctgcagttcg ccctcctcgt gccactcatt gccgccctgc
     7321 tgggcctcgt caactcgttc cggatggtcc ggctcggcga catcaagccg aaggcgaacc
     7381 tcgagggcgt ctcgctcggc tgagcgcctg tcggctcagc cggtgagcga gccgtcgcgc
     7441 aggagctggg tgtccaggcg gaagacgaag gccttcgcca gggcgatctc gatgcagacc
     7501 tcggcgcccg gaacgtgccc ctcgctgatg ctcaccaccc atggctgggc gttggtgtcc
     7561 acgctcgcgt tcgccacgcc gtagcactca gggacgccgc cccagaactg gacggtgagg
     7621 gtggcgccgt cgccgctgag caggtagccg gtgacggata ccgggatcgg attctcgacg
     7681 ccggccacga cgtccacgac ctcggcgtcg ggcgcgag
//
LOCUS       DASYGU010000152         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1208278, whole genome shotgun sequence.
ACCESSION   DASYGU010000152 DASYGU010000000
VERSION     DASYGU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208278"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VGO32_04130"
     CDS             complement(<1..424)
                     /locus_tag="VGO32_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7199972.1"
                     /translation="MRFTVLGRSPAVPNPGEACAGYLVEGGKSRVMVDVGPGTVAQLL
                     RLCNPWELDAVVVSHMHTDHFLDLVTMRYSFPWKDEANPKLKVYLPPGATAQMEHVAM
                     GAGLPDYFARSFAFIEHDGTAALEIGDLRLEPTPTVHFV"
     gene            complement(448..1773)
                     /locus_tag="VGO32_04135"
     CDS             complement(448..1773)
                     /locus_tag="VGO32_04135"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV7199973.1"
                     /translation="MTTDGRAGGKTLTRPEVASVHIGDQTTELPIVTGTEGERAADIG
                     QLRSDTGLVTFDPGYKNTAASASAITFLDGEAGVLRYRGYPIEQIAARSSFLETAYLL
                     IYGELPSTAEYEAWLDDIVHHTLIHEEMRRFFDSFPYDAHPMAVLASGTVGLSTFYQD
                     SHDPFNGEAVELTIRRLIAKVPTLAAWAYKKSIGQPYVYPRNDLDYVGNFLHMMFAVP
                     AEPYEVSSVISAALEQLLIMHADHEQNLSTATVRNAGSGQANLYACISAGMSALWGPL
                     HGGANESVIKTLERIAADGGNVAKWVEKAKDPDDRFKLPGFGHPVYRNFDPRANLIKA
                     TADRVLAALGADEELFDIAKRLEDTALSDDYFVERKLYPNVDFYSGLIYRAIGFPKNM
                     FTALFAMGRLPGWIAQWKEMIEDPETKIARPRQVYTGVPARDYVPIESR"
     gene            complement(1770..>2460)
                     /locus_tag="VGO32_04140"
     CDS             complement(1770..>2460)
                     /locus_tag="VGO32_04140"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004504866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEV7199974.1"
                     /translation="LLGINAGIFGAQGRSIAANAAEDVRILVVGNPCNTNCLIASRAA
                     PEIPRDRWFAMTMLDETRGRALLAQRAGAAVAEVRDLVIWGNHSSTQFPDAFHATIGG
                     RAAADVINDDTWLRGDFISSVQQRGAAIIAARGASSAASAANAVVETVRNVASPTGQI
                     FSAAMPSPGEELGYGIPEGLVFGFPLRGSQPGQIEIVQGISHDPWAAERIATTLAELI
                     EERDAVGELAA"
BASE COUNT          362 a          800 c          884 g          414 t
ORIGIN      
        1 gaacgaagtg aacggtcggg gtgggttcca gccgcaggtc accgatctcc agggccgcgg
       61 tcccgtcgtg ctcgatgaac gcgaacgatc gggcgaagta gtccggcagc ccggcgccca
      121 tggccacgtg ctccatctgg gccgttgccc ccggcgggag gtacaccttg agcttggggt
      181 tcgcctcgtc cttccacggg aaggagtaac gcatggtcac caggtccagg aagtggtcgg
      241 tgtgcatgtg gctgacgacc accgcgtcca gctcccacgg gttgcacagg cgcagcagct
      301 gggcaacggt cccgggtccg acgtcgacca tgacccggct cttcccgccc tccaccaggt
      361 agccggcgca cgcttctccc gggttgggca ccgcgggcga gcgtccgagg acggtgaagc
      421 gcacgggcgg gaggcgctcg gggaggccta gcgggactcg atcgggacgt agtcgcgggc
      481 ggggacgccg gtgtacacct ggcgcggccg cgcgatcttc gtctcggggt cttcgatcat
      541 ctccttccac tgcgcgatcc agccgggcag ccggcccatg gcgaacagcg cggtgaacat
      601 gttcttggga aagccaatgg cgcggtagat gagtcccgag tagaagtcga cgtttgggta
      661 cagcttgcgc tcgacgaagt agtcatcgct gagcgcggtg tcctccagcc gcttggcgat
      721 gtcgaacagc tcctcatcgg cgccgagagc ggccaggacg cggtccgcgg tggccttgat
      781 caggttggca cgcggatcga agttgcggta gaccgggtgg ccgaagcccg gcagcttgaa
      841 ccggtcgtcg ggatccttcg ccttctccac ccacttcgcg acgttgccgc cgtcggcggc
      901 gattcgctcc agggtcttga tcaccgactc gttggcgccc ccatggagcg gtccccacag
      961 cgcgctcatg ccagcgctga tgcaggcgta caggtttgcc tggccgctcc ccgcattgcg
     1021 caccgtggcg gtcgagaggt tctgctcgtg gtcggcatgc atgatcagga gctgctcaag
     1081 cgcggcactg atcaccgagc tgacctcgta cggctcggct ggcacagcga acatcatgtg
     1141 caggaagttg cccacgtagt ccaggtcgtt gcgcgggtag acgtacggct ggccgatgct
     1201 cttcttgtag gcccatgccg ccagggtcgg aaccttggcg atgaggcgcc ggatcgtgag
     1261 ctccaccgcc tccccgttga acgggtcgtg actgtcctgg tagaacgtgg acaggcccac
     1321 cgtgccgctg gccaggacgg ccatcgggtg ggcgtcgtac gggaacgagt cgaagaaccg
     1381 ccgcatctcc tcgtggatga gggtgtggtg gacgatgtcg tccagccagg cctcgtactc
     1441 cgccgttgac ggcagctcgc cgtaaatcag gaggtatgcg gtctcgagga agctcgaacg
     1501 ggctgcgatc tgctcgatcg gatagccgcg gtagcgcagc accccggcct caccgtcgag
     1561 gaaggtgatc gccgatgccg aggcggcggt gttcttgtag ccggggtcga aggtcaccaa
     1621 cccggtgtcg gatcgcagct gaccgatgtc ggccgcgcgc tcgccttcgg tcccggtgac
     1681 gatgggcagc tcggtcgtct ggtcgccgat gtggaccgat gcgacctccg gccgggtcag
     1741 ggtctttccg ccggcacgtc cgtcggtcgt catgcggcga gctctccgac cgcatcccgc
     1801 tcctcgatga gctccgcgag cgtggtggcg atccgctcgg cggcccacgg gtcgtggctg
     1861 atgccctgca cgatctcgat ctggcccggt tggcttcccc ggagcgggaa gccaaagacc
     1921 agtccctccg ggatcccgta gcccagctcc tcgccgggcg acggcatcgc ggccgagaag
     1981 atctggccgg tggggctggc cacgttgcgc acggtctcga ccacggcgtt ggcggccgag
     2041 gccgccgatg aggcgccgcg cgccgcgatg atcgcggctc ctcgctgctg gacggaggag
     2101 atgaagtcgc cgcgcagcca cgtgtcgtcg ttgatcacgt cggccgcggc gcgcccgccg
     2161 atcgtggcgt ggaacgcgtc ggggaactgg gtcgaagagt ggttgcccca gatcacgagg
     2221 tcgcggacct cggccacggc cgcgccggcg cgctgggcga ggagcgcgcg gccgcgggtc
     2281 tcgtccagca tggtcatcgc gaaccaccgg tcgcgcggga tctccggcgc cgcccgtgag
     2341 gcgatcaggc agttcgtgtt gcacgggttg ccgacgacca ggatccgaac gtcctcggcg
     2401 gcgttggccg cgatggagcg gccctgggcg ccgaagatgc cggcgttgat cccgagcagg
//
LOCUS       DASYGU010000153         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_899431, whole genome shotgun sequence.
ACCESSION   DASYGU010000153 DASYGU010000000
VERSION     DASYGU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899431"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1127)
                     /locus_tag="VGO32_04145"
     CDS             complement(90..1127)
                     /locus_tag="VGO32_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199975.1"
                     /translation="MLVWPTLRGTEVMVPWEGLLDRLDDLSPRIEALEEGVSLIDVTG
                     LEPMHGSERRVAARAVALAGMYPVRAGIGDNRWLASLAVRLARGEDRAACLILPAGEG
                     RATLAPMPIGLLPADQAVRDRFSLFGLTTMGQLAGLPRSAVAAQFGEVGERLQALARG
                     EDARPLAPRRRPEQLATSVRFESPAEGVTEITLALRRAATALTDRLAGRHLAPGRAVL
                     SLDRESAPPLAVHLALPEPSTDPDWITRLLLARLEAQLQGRAGGEEEVRITSLTLVLD
                     RLTNPAARQLPAFEPQAGRWEELRWSVERIGARFGNGRLWRAVVERPTAAFPERQARL
                     VEIGHEAGTQP"
     gene            complement(1159..1953)
                     /locus_tag="VGO32_04150"
     CDS             complement(1159..1953)
                     /locus_tag="VGO32_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199976.1"
                     /translation="MTLHAAQPLPEVLDALRERGLRRGVQPLPGEVEIPAVPTGHAAL
                     DGALATGGWPRGALAVLDAAPGHGATSLALDSAAASQAAGGLVAWIDLAGTFDPACAA
                     RHGVNLEWLLLVRPADVAEALELAAWLARSRLLDLLVLDLDGASAPRFERLAALLPRS
                     DATALLIGPPALRQAAGKVAGVRLRLERQSWLAVGRDLVGLRTAASVTRHRWALAGGH
                     AELDLWFGEGRRIDSLLPALAEPQPETVEQPYGLPEELPALAVLSA"
     gene            complement(2005..2532)
                     /locus_tag="VGO32_04155"
     CDS             complement(2005..2532)
                     /locus_tag="VGO32_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199977.1"
                     /translation="MSPDVGSGAGEAWTNVFAATIQPDLTITGDWGDVPFNPTLPSDV
                     MGNGSLVLRIDFDESDEVERPVLRTVAASGGFGGSVFVLEDSLSDPVELVGIFGGDDA
                     RECVWVEANGERTELVGSGAWVFRSPPLSIQDYSGHIAARVGDPIVVRGRLSTVLGGR
                     CTDTSMLVEELDPTP"
     gene            complement(2825..>3543)
                     /locus_tag="VGO32_04160"
     CDS             complement(2825..>3543)
                     /locus_tag="VGO32_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7199978.1"
                     /translation="LTILTVATYPAVRDMPGMEDLLEGLPDAILALIGESDLLSPEGY
                     LNSQLFGAIVPLLFLFFGVAAGAGAIAGEEENRTLDLLLSNPVSRSRVVIEKALAMVI
                     GLLLLATILWLATYLPAVAVDMGLDPANLVAAVVSASLFGFFMGGFALMVGAASGSRG
                     LAIGLGAALGIAAYLVNSLAPLVDGLDWLQQLSPFYYYYEADPLRNGLDPAHAAVLGV
                     GGLLFVLVAIIGFRRRDVGV"
BASE COUNT          579 a         1264 c         1264 g          436 t
ORIGIN      
        1 gtggtcctgg ccgtccagcc ggaggcggac gggcgtgccg gcctcgtcgg tgtcgacctg
       61 gaccagcgga tggctggcgt gcaaccgggt cacggctgtg tgccggcctc gtggccgatc
      121 tcgaccagcc gcgcctggcg ctcggggaac gcggccgtgg gccgctccac gaccgcgcgc
      181 cacaggcggc cgttgccgaa ccgggcgccg atccgctcga ccgaccagcg gagctcctcc
      241 catcggccgg cctgcggctc gaaggcgggg agctggcggg cggccgggtt ggtgagacgg
      301 tccaggacga gggtcaggga cgtgatccgg acctcctcct ccccgccggc cctgccctgc
      361 agctgggcct cgagccgggc gagcagcagg cgggtgatcc agtccgggtc ggtgctgggc
      421 tcggggaggg cgaggtggac ggccaggggc ggggctgact cccggtccag gctgaggacc
      481 gcccggcccg gggcaaggtg gcggccggca agccggtcgg tcagggccgt cgccgcgcga
      541 cggagggcga gggtgatctc ggtcacgccc tccgccggcg actcgaagcg gaccgaggtg
      601 gccagctgct ccggccggcg acgtggagca agcggccgcg catcttcccc gcgggcgagc
      661 gcctggagcc gctcccccac ctccccgaac tgggcggcca ccgccgaccg cggcagcccg
      721 gccagctggc ccatggtcgt cagcccgaac aggctgaacc gatcccggac ggcctggtcg
      781 gccggcagca gaccgatggg catcggggcg agcgtcgctc gcccctcacc ggcgggcagg
      841 atcaggcacg cggcgcggtc ttcgccgcgg gccaaccgga ccgcgagcga tgccagccat
      901 cggttgtccc cgatcccggc ccgcaccggg tacatcccgg ccagggccac cgcccgcgcc
      961 gcgacccgtc gttccgagcc gtgcatcggt tcgaggcccg tcacgtcgat gagtgacacc
     1021 ccctcttcca gggcctcgat ccgcggggag aggtcatcca gccggtcgag cagcccctcc
     1081 cacgggacca tgacctccgt gccccggagg gtgggccaga ccagcatcag gatccgcatt
     1141 ggtatgcctt ggagccggtc aggcgctgag gacggccagc gcggggagct cctcggggag
     1201 cccgtacggc tgctccacgg tctcgggctg cggctctgcc aacgccggca ggagcgagtc
     1261 gatccggcgg ccctcgccga accacaggtc gagctcggca tggccgccgg ccagcgccca
     1321 gcggtggcgg gtcactgatg ccgccgtgcg caggccgacg aggtcgcggc cgacggccag
     1381 ccaggattgc cgctcgagac ggagccggac gccggccacc ttgccggcag cttggcgcag
     1441 ggccggcggg ccgatgagca gggcggtggc gtcggatcgg ggcaggagcg cagccagccg
     1501 ctcgaagcgc ggggcggacg ccccgtccag gtccaggacg agcaggtcaa gcaggcggga
     1561 tcgggccagc catgcggcca gctcgagcgc ctcggccacg tcggcgggac ggacgaggag
     1621 cagccactcc aggttgacgc cgtggcgcgc ggcacaggcg gggtcgaacg tgccggccag
     1681 gtcgatccac gccacgaggc ccccggccgc ctggctggcc gcggccgaat cgagggccag
     1741 cgaggtggcg ccatgaccgg gagcggcatc caggacggcc agagcacccc ggggccagcc
     1801 accggtggcc agcgccccgt ccagggcggc gtggccggtg ggcacagcgg ggatctccac
     1861 ctccccgggg agcggttgga cgcctcgccg caggccgcgc tcgcgaagcg catcgagcac
     1921 ctcggggagc ggctgagcgg cgtggagggt cacatcggcc agtctaatag aacagatgtt
     1981 ctagatcaat ccccttcctg acggctaggg cgtcggatcg agctcctcga ccagcatcga
     2041 ggtatccgtg cagcggccgc cgagtaccgt ggacagcctt ccgcggacca cgatcgggtc
     2101 accgacccgc gccgcgatgt gacccgagta atcctgaatg ctgagcgggg ggctgcgaaa
     2161 tacccatgcc ccgctcccga ccagctccgt gcgctcgcca ttggcttcca cccagacaca
     2221 ctctcgtgcg tcatcgccgc cgaagatgcc aaccagctcg acgggatcgg aaagtgagtc
     2281 ctcgagcaca aataccgatc cgccaaaccc acctgacgcc gccactgtcc gcagcaccgg
     2341 gcgctccacc tcatccgatt cgtcgaaatc gatccgcagc accaacgacc cgttgcccat
     2401 aacgtcagaa ggaagcgtgg gattgaacgg cacgtctccc caatcgccgg tgatcgtcaa
     2461 gtcgggttgg atcgttgcag caaatacgtt ggtccacgcc tcacccgcac cggacccgac
     2521 gtccgggctc attcccaacc agtacaggca cgattcccgt tgaagaatgt cgtaccgacc
     2581 ctggtcgtca gctcgccaga cgccggtgag catcacgtgc cgtccggacg ggctgctgat
     2641 gtcgaggtcg gagcacgcac gtcctgccgg ctggaccgag gcgcccggtg cgagggaccc
     2701 ggagggtgac gcaccgctgc acgatgaagc gacaagggcc agcaggccga gtccggccag
     2761 ccggcgcgtt ctcacccgaa cagtctacga ccgggtttgc ggaatccgat tcgtgacgcc
     2821 gaagtcagac gccgacgtct ctccgtcgga agccgatgat cgccaccagc acgaacagca
     2881 gcccgcccac gcccaggacc gcagcatgcg ccggatcgag cccgttccgg agcggatcgg
     2941 cctcgtagta gtagtagaac ggcgacagct gctggagcca gtcgaggccg tcgaccaagg
     3001 gcgcgagcga gttcaccagg taggccgcga tccccagcgc cgcgccgagg ccgatcgcca
     3061 gcccgcgtga cccgctggcc gccccgacca tgagtgcgaa gccgcccatg aagaacccga
     3121 acagcgacgc gctgacgacc gccgccacta ggttcgccgg atcgagcccc atgtccaccg
     3181 ccacggccgg caggtaggtc gccagccaca ggatggtggc aagaagcagc agaccgataa
     3241 ccatggcgag tgccttctcg atcacgaccc ggctccggct caccgggttc gacaggagga
     3301 ggtccagggt ccggttctct tcctcgccgg caatcgcccc ggcccccgcg gccacgccga
     3361 agaacaggaa caggagcggc acgatggcgc cgaacagctg ggagttgagg tagccctcgg
     3421 gcgagaggag atcggactcg ccgatgagcg cgaggatggc gtccggcagg ccctcgagca
     3481 ggtcctccat gccgggcatg tcgcggaccg cgggataggt ggcaacggtc aggatcgtga
     3541 ggc
//
LOCUS       DASYGU010000154         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_128412, whole genome shotgun sequence.
ACCESSION   DASYGU010000154 DASYGU010000000
VERSION     DASYGU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128412"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1083)
                     /locus_tag="VGO32_04165"
     CDS             complement(112..1083)
                     /locus_tag="VGO32_04165"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7199979.1"
                     /translation="MRAFVTGGGGFIGLALIRQLRERGDEVVAIVRDPGPATILEELG
                     CTLVAGDLGAMDAVRLTELMAGVSAVFHLAGVYRVGIAESEHVAMFTANVVATRAVLD
                     AAVAADVARTIYTSTVNTFGDTHGQVVDETYRRPQPPAFLSYYDETKFLAHQAAEERI
                     AADAPVLIAIPGMVYGPGDHSQAGGVIREAMAGTLSVLSAADLGGSFVHVDDVAAGIV
                     LVHDRGAIGQSYVLGGELATLREVVRRAAALAGREPPKITTPSWLMQALAPVAEAIGP
                     LLPGDEPNVAEMIRASNGVTYWASSEKAKRDLGYQPRDLEAGLRTLL"
     gene            1176..2021
                     /locus_tag="VGO32_04170"
     CDS             1176..2021
                     /locus_tag="VGO32_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199980.1"
                     /translation="MISNMSSRALPMAVVGLSLVFFWVLVAFALAGGGNLTDPAEILT
                     RGAQATAHADSFHFSMSVEGSIIDADSGDPVPLDGVSVQGDVDVAGEAAQVAFSLPML
                     FGMEGEVIVIGEDVYLLTAMAGDQWLHLPASPDEETEPLGDPPTDEEIADKVNEFVTT
                     DGVGVSLLADQACGDDTCYHLQVSVSEEALAAHQGEMPDMGAPEMGGVGEMLPNPQFS
                     GPAVVDLLFDKNGLWLRSIVVASEGEAGAASFTVDFIEYDTSFDISAPPADQVIEADD
                     FPFFQ"
BASE COUNT          337 a          737 c          721 g          336 t
ORIGIN      
        1 gagatggttc gccaggctac gtcgctcatc gggacctcca tcggacactg caggcgcacg
       61 catgatgtgc aatctgccgg cggtctgcgt tcggtttcga catggtgcgg gtcagagaag
      121 ggttcgcagg ccggcttcca ggtcacgggg ctggtagccg aggtcgcgct tcgccttctc
      181 gcttgacgcc cagtacgtga ccccgttcga ggctcggatc atctcggcca cgttcggctc
      241 gtcgccgggc agcaacggac cgatggcctc cgccaccggc gcgagggcct gcatcagcca
      301 cgacggggtc gtgatcttcg gcggctcccg gccggccagg gccgccgcac gtcggacgac
      361 ctcgcgcagg gtggccaact cgccgccgag gacgtaggac tggccgatcg caccgcggtc
      421 gtggaccagg acgatcccgg cggccacgtc gtccacatgg acaaagcttc ccccgagatc
      481 cgcggcgctg aggaccgaca acgtgccggc catcgcctcg cgaatgacgc cgcccgcctg
      541 ggaatgatcg ccagggccgt agaccatccc cggtatggcg atcaggacgg gagcgtcggc
      601 cgcgatccgc tcctcggccg cctgatgggc gaggaatttc gtctcgtcgt agtaggagag
      661 gaaggcaggt ggctgaggcc gccggtacgt ctcgtccacc acctgaccat gcgtgtcccc
      721 gaacgtattc acggtcgacg tgtagatcgt gcgggccacg tcggcggcga cggccgcgtc
      781 gagcacggcc cgggtggcga ccacgttcgc ggtgaacatc gccacgtgct cggactccgc
      841 gatgcccacc cggtagaccc cggccaggtg gaagacggcg gacacaccgg ccatcaactc
      901 ggtgagccgc accgcatcca tggcgcccag gtcgccggcg accagcgtgc agccaagctc
      961 ctcgagaatg gtcgcgggac cgggatcacg aacgatggcc acgacctcgt cgccgcgctc
     1021 acgcagctgg cggatgaggg ccaggccgat gaacccgcca ccaccggtca cgaatgccct
     1081 cacggagcgc tcgatcggac gtccatggcc cgcatcatga cgcggcagga ccatcggcac
     1141 atggcgggaa atcggggacc ggctatcctg ccccggtgat cagcaacatg agctcgcggg
     1201 cgttgcccat ggccgtcgtc gggttgagcc tcgtcttctt ctgggtcctc gtggcgttcg
     1261 cgctcgccgg aggcgggaac ctcaccgatc cggccgagat cctgacccgc ggtgcgcagg
     1321 ccaccgccca cgccgacagc ttccacttct ccatgtccgt ggagggcagc atcatcgatg
     1381 ccgatagcgg cgacccggtt ccgcttgacg gcgtcagcgt ccagggcgac gtcgacgtcg
     1441 ccggcgaggc cgcgcaggtc gctttctcgc tgcccatgct gttcggcatg gagggcgagg
     1501 tcatcgtcat cggcgaggac gtctatctcc tgaccgcgat ggccggcgac caatggctcc
     1561 atctgcccgc cagcccggac gaagagaccg agcctctggg agacccgccg accgacgagg
     1621 agatcgcgga caaggtcaac gagttcgtga ccaccgatgg cgtgggtgtc tctctcctcg
     1681 ccgaccaggc gtgcggtgac gacacctgct accacctcca ggtctcggtt tccgaagagg
     1741 cgctcgctgc gcaccagggt gagatgccgg acatgggtgc gccggagatg ggtggcgtcg
     1801 gggagatgct cccgaatccc cagttcagcg ggccagcggt cgtggacctc ctgttcgaca
     1861 agaacggcct gtggctccgc tcgatcgtgg tcgccagcga aggcgaggcg ggagcggcca
     1921 gtttcacggt cgacttcatc gagtacgaca cgtcgttcga catcagcgcc ccgcccgccg
     1981 accaggtcat cgaggccgac gacttcccct tcttccagtg atctgagccg cgccccgagc
     2041 gcggtgtggg tgagcctcct gtccgcgtgc tgctgacggg tgcccgggcg caccgcttct
     2101 ttgccgaccc ccgttgacga cggaccggcg g
//
LOCUS       DASYGU010000155         8784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_899924, whole genome shotgun sequence.
ACCESSION   DASYGU010000155 DASYGU010000000
VERSION     DASYGU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899924"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..572)
                     /gene="cysC"
                     /locus_tag="VGO32_04175"
     CDS             complement(36..572)
                     /gene="cysC"
                     /locus_tag="VGO32_04175"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEV7199981.1"
                     /translation="MTGCTVWFTGLSGAGKTTIADLVEAELRRRGHKVEVLDGDVVRT
                     HLSKGLGFSKEDRDTNIRRIGWVCEVLTRNEIIAIAAAISPYREVRDEVRANIRDFVE
                     VYVEAPIDVLAERDVKGLYKKALAGEISGFTGVSDPYEPPFEPEVICHTDGRETPEES
                     AAKVIRALEERGYSTAPT"
     gene            complement(569..1744)
                     /gene="sat"
                     /locus_tag="VGO32_04180"
     CDS             complement(569..1744)
                     /gene="sat"
                     /locus_tag="VGO32_04180"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEV7199982.1"
                     /translation="MNHALIPPHGGELIDRVLTGSDREAAIERAASLPRLRLGAVERS
                     DLELIGNGAFSPLTGFMTRTDYTSVVDGMRLANGLPWTIPITLAVSRQEAEALPVGSS
                     VALTDEGERVVGILEIADAFEYDKDVEAERVFRTTDVSHPAVERIRAAGEVYLGGDVQ
                     VLELPLDPPFSSVRLTPSETRSAIAERGWRRVVGFQTRNPIHRAHEYLTKVALEITDG
                     LLIHPLVGETKSDDTPAAVRMQAYQALLSNYYPADRTMLAVYPAAMRYGGPREAIFHA
                     IARKNYGCTHFIVGRDHAGVGNFYGSYDAHLIFDEFAPGELGITPLFFEHSFWCRACE
                     GMASSKTCPHPETERVTLSGTRVRELLTAGEMPPPEFSRPEVAQVLIDAYRTEIPVR"
     gene            1892..2923
                     /locus_tag="VGO32_04185"
     CDS             1892..2923
                     /locus_tag="VGO32_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV7199983.1"
                     /translation="MVGPASPAPSPALAGTDAAAFAERFRAAKSVTTFCHEQPDGDTL
                     GAAVAMALIAARLGANTEVVSVDGVPPAYGFLARAEEIRRAPLLAPDLAIVCDAATFE
                     RIGPVLSSSADWFAQATVANIDHHATNAMFGAINVVDETAAATCQVVADLLPLLGVPL
                     DEEIATALLAGLIRDSHGFSTSSTSPRTLRAAADAVSAGASIDAISRATLLELPIPTM
                     RLWSRLLAGMETEFDDQLIYAVLDRDGLADTGTEQHDADGVVEFLARGRGIRVAILFR
                     ELDGFTRVSLRTAQDVDAAAIAGAFAGGGHRRRSGCTIQAPTAKAIPLVLDACRPYLR
                     PEHPRAEDA"
     gene            complement(2889..3251)
                     /locus_tag="VGO32_04190"
     CDS             complement(2889..3251)
                     /locus_tag="VGO32_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199984.1"
                     /translation="MHVVVLVSDLITASRIESVVIRSEGTFDRIESPEALAAFAEADL
                     LVVDWAAREPRWAAAISTWREGGPEPRPRVILFGPHTDLEAHAEARASGLGPMLARSA
                     LSGEVERVMRLRRGDAPA"
     gene            complement(3269..3658)
                     /locus_tag="VGO32_04195"
     CDS             complement(3269..3658)
                     /locus_tag="VGO32_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199985.1"
                     /translation="MSAAKRPSASSLRRFVSSRPYVPVAELRRRFGLEEPDDMIAISR
                     NGVTAFIGLPAREAAKLEELWVRDEVGLELSVDVRAPVVVGVFPLRIARYAVDHVGAN
                     GTSDVTGPAGTAPYAPPLEGQPVRPVS"
     gene            complement(3669..4040)
                     /locus_tag="VGO32_04200"
     CDS             complement(3669..4040)
                     /locus_tag="VGO32_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV7199986.1"
                     /translation="MQVELLFWDADPEYMTARQRLVEVLIEDAFETPIQMIAVSSVAD
                     AEFLALPGSPTIRIDGADIDPEGVGEIGLYRRRYQADDGSSDPAPSKALIRLAVERAR
                     GWSHGRPEASAPPTTSESGPG"
     gene            complement(4058..4318)
                     /locus_tag="VGO32_04205"
     CDS             complement(4058..4318)
                     /locus_tag="VGO32_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199987.1"
                     /translation="MTWAFLAIALVSGAVGLAVGLPAWRSWQARAARGRNAERYLTWR
                     GRGDPPETADMQATSAERRRIAVGAALGAAAIACLVIGLAAG"
     gene            complement(4315..5049)
                     /gene="pgl"
                     /locus_tag="VGO32_04210"
     CDS             complement(4315..5049)
                     /gene="pgl"
                     /locus_tag="VGO32_04210"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV7199988.1"
                     /translation="MTVTFSVLPGPDDVAHAAATAFVEASRAAVAARGRFYAAIPGGR
                     TPLPAFVLLTGPAFHDRVPWERVELFWADERAVGPDDPQSNYRAAREVLLDHAPSLST
                     GQVHRMMAEAVDLETGARAYEAELRRVVPAGPDGIPVLDLVWLGVGADGHTASLCPND
                     PSLAVTDRLVVPTWPAGYDTARLTLTYPVLNAAREIVFAVNGADKAETLAAIRAGADL
                     PAARVRAAQTRWLVDAAAAGQVADPV"
     gene            complement(5046..6269)
                     /locus_tag="VGO32_04215"
     CDS             complement(5046..6269)
                     /locus_tag="VGO32_04215"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEV7199989.1"
                     /translation="MSLEPGATPYWGMRGTTVLAIEAELGRLWHQATADPAKDGMVVT
                     EKGATSARTSVMNLIVKVPDQAASDHIMATLLSLGTRHPSRAIVLQADPQADGEAIDA
                     RIGTHCRDGENGAGRVCFEQLSLTIRGEAARHLAGIVAPLVIHDLPIDVWWPGDPPFD
                     DPVFTQLLELGDRLVVNASDFGDLPGGLKQLAVIRRRAGVGDITWERLSPWLELTAQF
                     FDAPRFRRYLPNLSRLSVRYAVPDPAAGPVKNRDPGTGEAIQGAQAPAAAALLYAGWI
                     ATRLAWRRAQWREPSTPGALRLVLEGRYEMVDLALDPVPTSAVAPGELLSVRLRAFGE
                     AGSAEFIIDRPAAEATAVTNADGMTAYLRRIPLAPESEAEVLRRQLVLDERDLVYEDA
                     LRAAAILLATASDAA"
     gene            complement(6266..7840)
                     /gene="zwf"
                     /locus_tag="VGO32_04220"
     CDS             complement(6266..7840)
                     /gene="zwf"
                     /locus_tag="VGO32_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV7199990.1"
                     /translation="MSVQAPAPVTTSVNPLRQGLRQERMPEPCTMVICGATGDLTRRK
                     LAPAIYNLALGGFLPPEFTVVGFARRPMSNEEFHAHLREGIDQFSRNRPASDSVWSSF
                     ATGVEYQQGNLDDPEAYADLARRLDRIDRDRGTAGNRLFYLAIQPSLVPDVVHQLGAA
                     GLAGSSERTSGRLGWTRVVVEKPFGSDIESAKTLNAQINEVFREDQIYRIDHYLGKET
                     VQNLAVFRFGNGLFEPIWNRRYIDSVQITVAETVGLEGRGEFYDQTGALRDIVQNHVL
                     QLLAVFAMEPPVEFAPNDLRDEKLKVLRAVRPMSPEDVAHNTVRGQYITGWVAGEKVP
                     AYRDEPEVAPDSETETYVGLKLGIDSWRWAGVPFYIRTGKALASRVTEIAVTFRRAPL
                     ALFGRAGAPDMDPNVLAIRVQPDEGILLRFGAKVPGPGLNIRGVNMDFRYGSSFAVDS
                     PDAYETLLLDCMIGDSTLFTRDDEVVRAWEILDPIVRTWQAGQGGPIHFYAAGSWGPP
                     AADELLARDGREWRRP"
     gene            complement(7844..>8784)
                     /gene="gnd"
                     /locus_tag="VGO32_04225"
     CDS             complement(7844..>8784)
                     /gene="gnd"
                     /locus_tag="VGO32_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEV7199991.1"
                     /translation="MQVGIVGLGRIGAGMARRLARGGHEVVAWNRTASVATDLAAEPE
                     NGGKVTGVGTVESLAEALSTPRHVLLSLPAGDATQELIDRLVPLLADGDVIVDTGNAN
                     FRDSARRATELAAHGLTFMDMGVSGGVWGLEVGFCAMVGGDRAAFARLEPAIATLAPP
                     DGYLYCGPAGAGHYVKMVHNGIEYGLMEAYGEGFDILHASDYDLDLGAVAHLWNHGSV
                     IRSWLLELAANAFEKEGNDLEGIAGWVADSGEGRWTVADALEHDVPAPIITLALIQRL
                     RSRRTPDSFTDRVLAALRNEFGGHAVKSTGPTDGPT"
BASE COUNT         1362 a         3011 c         3090 g         1321 t
ORIGIN      
        1 gacctcggcg accatgaatt tggcccagat ttcgctcatg tgggggcggt ggagtacccc
       61 cgctcttcca gggcgcgaat gaccttcgcg gcgctctcct cgggcgtttc ccgcccatcg
      121 gtgtggcaga tgacctcggg ctcgaagggc ggctcgtatg ggtcacttac gccagtgaaa
      181 ccactgatct cgccggcgag ggccttcttg tacagccctt tgacatcgcg ctcggcgaga
      241 acgtcgatcg gggcctcgac gtagacctcg acaaagtcgc ggatgttggc ccgaacttca
      301 tcgcggacct cgcggtacgg gctgatggcg gcggcgatgg cgatgatctc gttgcgggtg
      361 aggacctcgc acacccagcc aatgcgccgg atgttcgtgt cgcggtcctc cttcgagaac
      421 ccgagcccct tggacaggtg ggtgcgcacg acgtcaccat cgaggacctc gaccttgtgg
      481 ccacgtcgtc gcagctccgc ctccaccagg tcggcgatgg tcgtcttgcc ggcacccgac
      541 agaccggtga accagaccgt gcacccggtc atcggaccgg gatctcggtg cggtaggcgt
      601 cgatcaggac ctgggccacc tcgggtcggc tgaactcggg cggtggcatc tcgcccgcgg
      661 tgagcagctc gcgaacccgt gtgccagaaa gcgtgacccg ctcggtctcc gggtgcgggc
      721 acgtcttcga tgaagccatg ccttcgcacg cacggcacca gaacgaatgc tcgaagaaga
      781 gcggcgtgat tcccagctcg ccgggcgcga actcgtcgaa gatcagatgg gcgtcatacg
      841 agccgtagaa gttgccgacc ccggcgtgat cgcggccgac gatgaagtgc gtacagccgt
      901 agttcttccg cgcgatggca tggaagatcg cctcgcgggg gccgccatag cgcatggccg
      961 cgggatatac ggccagcatc gtgcgatcgg cggggtagta attcgacagc aatgcctggt
     1021 acgcctgcat ccggaccgcc gccggcgtgt cgtctgactt cgtttcaccg accagcggat
     1081 ggatgaggag gccgtcggtg atttcgaggg ccactttggt gaggtactcg tgggcgcgat
     1141 ggattggatt ccgcgtctgg aacccgacga cgcggcgcca tccacgctcg gcgatcgccg
     1201 atcgagtttc ggacggagtc agccgaacgg aggaaaacgg aggatcgagc gggagctcga
     1261 gcacctggac atcgccgccg aggtagacct cgccagccgc tcggatgcgc tcgacggccg
     1321 ggtgactgac atcggtcgtt cggaacacgc gttcggcttc gacatccttg tcgtactcga
     1381 acgcgtcagc aatctcgagg atgcccacca cccgctcacc ctcatcggtc agggccaccg
     1441 atgagccgac ggggagcgcc tcggcttctt ggcgactgac ggcgagggtg atcgggatgg
     1501 tccacggaag accgttcgcc aagcgcatgc catcgaccac cgaggtgtaa tcggtacggg
     1561 tcatgaatcc ggtcagcggg ctgaaggcgc cgtttccgat cagctcgaga tccgagcgct
     1621 cgacggctcc gagtcggagg cgaggtagcg atgcggcgcg ctcgatggcg gcctcgcgat
     1681 cggaaccggt caagacccgg tcgatgagtt caccgccgtg cggcgggatg agggcgtggt
     1741 tcaatgcggg ggcctctggc gtggaatggg ctgcgatagt acccgacccg cgtccgaggg
     1801 cgccgatccg ggtccacggg gtggccgcgg gattggctgg ctagaatcac atctcgtcaa
     1861 tttcgcgagc gcggagccgg gaggattacc gatggtcggt ccagcctcac ccgccccgtc
     1921 gcctgcgctc gcggggacgg acgcggccgc atttgccgag cgctttcggg cggccaagtc
     1981 ggtgaccacg ttctgccatg agcagcccga cggcgacacg ttgggcgcgg ccgtggccat
     2041 ggccctgatc gcagctcgac tgggcgcgaa caccgaggtc gtctcggtgg acggcgtccc
     2101 gcccgcgtac gggttcctgg cccgggcgga ggagatccgg cgcgcgcccc tgctcgcgcc
     2161 ggacctcgcg atcgtgtgtg atgcggccac cttcgagcgc atcggcccgg tcctcagctc
     2221 ctcggcggat tggttcgcgc aggccacggt ggccaacatc gaccaccacg cgacaaacgc
     2281 aatgttcggg gcaatcaacg tcgtcgacga aaccgcggcc gcgacctgtc aggtggttgc
     2341 cgaccttctg ccgctgctcg gcgttccgtt ggacgaggag atcgccacgg cgctgctggc
     2401 cggcctgatc cgcgacagcc acggattctc gacgagcagc acatcgccgc ggacgctgcg
     2461 agccgccgcg gatgcggtgt cggccggcgc gtcgatcgac gcgatctcgc gcgcgacgct
     2521 cctcgagctc ccgatcccga ccatgcgcct gtggtccagg ctgctggccg ggatggaaac
     2581 cgaattcgat gatcagctga tctacgccgt gctcgaccgg gatgggttgg cggataccgg
     2641 caccgagcag cacgacgccg acggagtggt cgagttcctc gcgcgcggtc gcgggatccg
     2701 ggttgcgatt ctcttccgcg agctcgatgg gttcacccga gtcagcctgc gcacggcgca
     2761 ggatgtggat gcggccgcga ttgccggagc gttcgcgggg ggcggccatc gtcgacggtc
     2821 ggggtgcacc atccaggcgc cgacggcgaa ggcgatcccc ctcgtgctgg atgcctgccg
     2881 gccctacctc aggccggagc atccccgcgc cgaagacgca tgacccgctc gacctcgccg
     2941 ctgagcgccg atcgggccag catcggaccc agcccggagg ctcgcgcttc cgcgtgagcc
     3001 tcgaggtcgg tgtgcggccc gaacaggatc accctgggac gtggttcggg tccgccctct
     3061 cgccaggtgg agatcgctgc ggcccatcgc ggctcgcgcg ccgcccagtc gacgacgagc
     3121 aggtccgctt cggcgaatgc ggcgagcgcc tccggcgatt cgatgcgatc gaacgttcct
     3181 tcggaccgga tcacaacact ctcgatccgc gacgcggtga tgaggtccga gaccaggacg
     3241 acgacgtgca cggtcgggcc gcggtgggct agctgaccgg tcggaccggc tgaccttcga
     3301 gcggcggggc atacggggct gtcccggccg gcccagtgac gtcggacgtg ccgttggcgc
     3361 cgacgtggtc gacggcgtag cgggcaatcc ggagggggaa gacaccaacc acgacgggcg
     3421 cccgaacgtc gaccgagagc tccaacccga cctcgtcgcg aacccacaac tcctcgagct
     3481 tggcggcctc ccgcgccgga agcccgatga acgcggtcac tccattgcgg ctgatcgcga
     3541 tcatgtcgtc cggctcctcg agcccgaacc ggcggcgcag ctcggccacc ggcacgtacg
     3601 ggcgggagct gacgaaacgg cgcaggctgg acgcgctggg ccgcttggcg gcggacatgt
     3661 gcccgagtct accccgggcc cgactcagac gtggtgggcg gtgctgaagc ctccggacgg
     3721 ccgtggctcc atccgcgggc ccgctccacg gcgaggcgaa tgagcgcttt cgacggggcc
     3781 ggatctgagg aaccgtcgtc ggcctggtac cggcgccgat acaggccgat ctcgccgacc
     3841 ccctcggggt cgatatcggc gccgtcgatc cggatggtcg gtgagccggg caacgccagg
     3901 aactcggcgt cggcgaccga ggagaccgcg atcatctgga tcggcgtctc gaacgcgtcc
     3961 tcgatgagca cctcgaccag ccgttgccga gcagtcatgt actccggatc cgcatcccag
     4021 aacagcagct cgacctgcat cggaccgcgc cggtcgctca gccggccgcc aggccgatga
     4081 ccaggcaggc gatggccgcg gcaccgagcg ccgcgccgac cgcgatccga cggcgttcgg
     4141 ccgaggtcgc ctgcatgtcg gcggtttcgg gcgggtcccc gcgcccccgc cacgtgagat
     4201 agcgctccgc attccgacca cgagctgccc gtgcctgcca ggaccgccac gcgggcaggc
     4261 cgaccgccag cccgacagcc ccggagacca gcgcaatcgc gaggaacgcc caggtcatac
     4321 cgggtcggcc acctgccccg ccgcggcggc atccaccagc cacctggttt gcgcggcacg
     4381 aacccgggcg gccggaagat cggctcccgc cctgatcgcg gccagggttt ccgccttgtc
     4441 cgcaccgttc accgcgaaga cgatctcgcg cgcagcattg agcaccggat aggtgagcgt
     4501 gagccgggcc gtgtcgtagc cggctggcca ggtagggaca acgagacggt cggtcacggc
     4561 cagggaagga tcgttggggc agagggaggc agtgtgccca tccgcgccca cgcccagcca
     4621 gacgaggtcc aggacgggga tgccatccgg cccggccgga acgacgcggc gtagctcggc
     4681 ctcgtaggcg cgggccccgg tctcgagatc cactgcctcg gccatcatcc ggtgcacctg
     4741 tccggttgac aggctgggtg catggtcgag gaggacctcg cgcgcggcgc ggtaattcga
     4801 ctgcggatca tccgggccga ccgcccgctc atcggcccag aacagctcga cccgctccca
     4861 tggcacacgg tcatggaatg ccgggccggt gagcagcacg aatgcgggga gcggcgtccg
     4921 gccacccgga atcgccgcgt agaagcggcc gcgggctgcc accgcggccc gcgaagcctc
     4981 gacgaacgca gtggcggcgg catgcgcgac atcgtcgggc ccgggcagga cggaaaaggt
     5041 aacggtcacg ccgcgtccga tgcggtggcg agcaggatcg ccgcggcgcg cagtgcgtcc
     5101 tcgtagacga gatcccgctc atccagcacc agctggcggc ggagcacctc ggcctcgctc
     5161 tcgggcgcga gcgggatgcg gcggaggtag gcggtcatcc cgtcggcgtt cgtcaccgcg
     5221 gtggcctcgg ccgccggccg gtcgatgatg aactcggccg agcccgcttc accgaacgcg
     5281 cggaggcgca ccgacaacag ctctccgggg gcgacggctg acgtgggcac cggatcgagc
     5341 gcgaggtcga ccatctcgta gcggccctcg aggacgagcc ggagggctcc gggggtcgag
     5401 ggctcgcgcc actgcgcccg acgccacgcc agcctggtgg cgatccagcc ggcatacagc
     5461 agcgcggccg cggcgggcgc ctgggcgcct tggatcgcct cgccggtccc ggggtcacgg
     5521 ttcttgaccg gaccggccgc cggatcgggc accgcgtagc ggaccgagag ccgggagagg
     5581 ttgggaaggt accggcggaa gcgcggggcg tcgaagaact gggcggtgag ctcgagccac
     5641 ggcgacaggc gctcccaggt gatgtcgccc acgcccgcac ggcggcggat gaccgccagc
     5701 tgcttgaggc cgccgggcag atcgccgaag tccgaggcgt tcaccacgag ccgatcgccg
     5761 agctcgagga gctgggtgaa caccgggtcg tcgaacgggg ggtcccccgg ccaccagacg
     5821 tcgatcggca ggtcgtggat cacgagcggc gcgacgatgc cggccaggtg tcgggcagcc
     5881 tcgccgcgga tcgtcaagga gagctgctcg aagcacacgc ggcccgcgcc gttttcgccg
     5941 tcccggcagt gggtgccgat ccgggcatcg atcgcctccc cgtctgcctg cggatcggcc
     6001 tggagcacga tcgcacggga gggatggcgg gtgcccaggc tcagcagggt ggccatgatg
     6061 tggtcgctgg ccgcctggtc aggcaccttc acgatcaggt tcatgaccga ggtccgggcg
     6121 ctggtggcgc ccttctcggt caccaccatc ccgtccttgg ccgggtccgc ggtcgcctgg
     6181 tgccacagcc gcccgagctc ggcctcgatg gcgaggaccg tggtcccgcg catcccccag
     6241 tacggtgtcg cgcccggctc gaggctcacg ggcggcgcca ctccctcccg tcacgcgcga
     6301 gcagctcgtc cgcggccggc ggaccccacg acccggcggc atagaagtga atcggcccac
     6361 cctggccggc ctgccacgtc cggacgatgg ggtcgaggat ctcccaggcc ctgaccacct
     6421 cgtcgtcgcg ggtgaacagg gtcgagtcgc cgatcatgca gtcgaggagc agcgtctcgt
     6481 atgcgtcggg cgagtcgacg gcgaacgagc tgccgtaccg gaagtccatg ttcacgcccc
     6541 ggatgttgag acccggcccg gggaccttgg caccaaagcg gagcaggatg ccctcatcgg
     6601 gctggacccg aatggcgagg acgttggggt ccatatccgg cgccccggcg cgaccgaaca
     6661 gggcgagcgg ggcgcggcgg aaggtgaccg cgatctcggt cacccgtgac gcgagggcct
     6721 ttccggtccg gatgtagaac gggacgcccg cccagcgcca cgagtcgatt ccaagcttga
     6781 ggccgacgta cgtttcggtc tccgagtcgg gtgcgacctc gggctcatcg cggtaggccg
     6841 ggaccttctc cccggccacc cagccggtga tgtactggcc gcgcaccgtg ttgtgggcca
     6901 cgtcctcggg tgacatcggc ctgacggcgc ggagaacctt cagcttctcg tcgcgcaggt
     6961 cgttcggcgc gaattcgacc ggtggctcca tggcgaacac ggcgagcagc tggagcacgt
     7021 ggttctggac gatgtcacgc agggcgccgg tctggtcgta gaactcgcca cgcccctcga
     7081 ggcccacggt ctcggccacc gtgatctgga ctgaatcgat atagcggcgg ttccagatcg
     7141 gctcgaacag gccgttcccg aatcggaaca cggccaggtt ctggaccgtc tccttgccga
     7201 ggtagtggtc gatccggtag atctggtcct ctctgaacac ctcgttgatc tgcgcgttca
     7261 gcgtcttcgc ggactcgata tccgatccga acggcttctc gacgaccacc cgcgtccaac
     7321 ccaggcggcc gctcgtccgt tcgctgctgc ccgccaggcc ggcggcaccc agctggtgga
     7381 caacgtcggg gacaagtgaa ggctggatgg cgaggtagaa gagccgattg ccggcggtgc
     7441 cccggtcgcg gtcgatccgg tcgagccgcc gggcgagatc ggcgtaggcc tcggggtcgt
     7501 cgaggtttcc ctgctggtac tcgacccccg tcgcgaacga gctccagacc gagtcggacg
     7561 ccggtcgatt ccggctgaac tggtcgatgc cctcccgaag gtgggcgtgg aactcctcgt
     7621 tcgacatcgg acgccgggcg aacccgacca ccgtgaactc cgggggcagg aagccaccca
     7681 gcgccaggtt gtagatcgcc ggtgccagct tccgacgggt gagatcgccg gtcgcgccgc
     7741 agatgaccat cgtgcacggc tcgggcatcc gctcctggcg gagaccctgg cgaagggggt
     7801 tgaccgaggt cgtgaccggt gccggcgcct ggacgctcat cggtcaggtc gggccatcgg
     7861 ttggtccggt ggacttcacc gcatggccgc cgaactcgtt tcggagcgcg gccaggaccc
     7921 ggtccgtgaa gctgtccggc gtccggcgac tgcggaggcg ttggatcagg gcgagggtga
     7981 tgatcggggc cggcacgtcg tgctcgagcg catcagccac cgtccatcgg ccctcgccgg
     8041 agtccgcgac ccagccggcg atgccctcca ggtcattccc ctccttctcg aaggcgttcg
     8101 ccgccagctc gagcagccag gatcggatca cgctgccgtg gttccacaga tgggcgacgg
     8161 cgcctaggtc gaggtcgtag tcggaggcgt ggaggatgtc gaagccctcg ccgtacgcct
     8221 ccatcaggcc gtactcgatc ccgttgtgga ccatcttcac gtagtggccg gcaccggccg
     8281 gtccgcagta caggtagcca tcaggcgggg caagggtggc aatcgccggc tcgagcctcg
     8341 cgaaggcggc gcgatcgcca ccgaccatcg cgcagaaacc aacctccagc ccccacactc
     8401 cgcccgaaac gcccatgtcc atgaaggtca ggccgtgcgc ggccagctcc gtggcgcgcc
     8461 gggcgctgtc gcggaagttc gcgtttccgg tgtccacgat cacgtcgcca tccgccagca
     8521 gcgggacgag acggtcgatg agctcctggg tggcgtcacc ggccggcagg ctgaggagca
     8581 catgccgcgg ggtggaaagc gcttccgcca ggctctccac cgttccgacc ccggtcacct
     8641 tccccccgtt ctcgggctcc gccgccaggt cggtcgccac cgaggccgtt cggttccatg
     8701 ccacaacctc gtggccgcca cgggccaggc ggcgcgccat ccccgccccg atccgcccca
     8761 gtcccacgat gccgacctgc atga
//
LOCUS       DASYGU010000156         4778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1105988, whole genome shotgun sequence.
ACCESSION   DASYGU010000156 DASYGU010000000
VERSION     DASYGU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105988"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VGO32_04230"
     CDS             complement(<1..580)
                     /locus_tag="VGO32_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEV7199992.1"
                     /translation="MDFRALVDHLLAEFFRLDPVHATEIGNHEHDGAWPDLSPAGAEI
                     RRAFAQDALRQLGEIDRASLDRDDAIDHRILTDALAAMEFGETTLQEERWNPLVYVYV
                     LGNGLFALLARPFAPLPERLASAASRLRGVPAVLDAARANLLDPGDRPVARFHTEKAI
                     ERLPGIPGLVDAVLAEVDKVEDADAAAALRAEL"
     gene            636..1787
                     /locus_tag="VGO32_04235"
     CDS             636..1787
                     /locus_tag="VGO32_04235"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV7199993.1"
                     /translation="MADVPDRSAYLAEARAILARHPLIDGHNDLAWEIRKYPAAPGDL
                     DAYDLRVRAPAPGDTDIPRLREGGVGGQFWSVYVPGEGQAPRAVLQLEQLDLARRMVA
                     HYPDDMALCLTADDVDHALAEGRIASLLGMEGGHVLEGSLGALRAFWELGARYLTLTH
                     NTANEIGDSGTDAPQHGGLSPFGRQVVAEMNRLGMLVDLSHTSVETMSDALDATQAPV
                     IFSHSSARALCDVARNVPDEILRRMPANGGVVMVTFVAGFIGPEAAAILIPAIAEFGR
                     RAAGIQDVAVREALAREIIDSLTVPSPPLELVADHVDHVARVAGHDHVGIGGDYDGNT
                     FWPVDLEDVSCYPNLIAELLRRGWTEEDLGKLANGNIRRVLRDAEAAAG"
     gene            complement(1809..3200)
                     /locus_tag="VGO32_04240"
     CDS             complement(1809..3200)
                     /locus_tag="VGO32_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7199994.1"
                     /translation="MVDPASFRDPGGYIFRRDGVLYRAIQPVAAADWSAFEDRGLAAE
                     LVGAGLLVGYAPVSVDLAPRPGAALVIRPRELPIISYPYEWAFSQLREAALLTLEVQR
                     RALSAGMWLRDASAYNVQVDAGRPILIDSLSFEVADLSAPWPAYRQFCKHFLAPLALM
                     AHRDPRCGLMLRDFIDGLPLDLTTALLPGRTRIGGLLPHLHLHAGADRRAQRAEIQGA
                     SEPGATQSRARPMNRTRHEALLDSLRRAVEGLNLELGSHWLSYTRQTSYTEAAAASKR
                     EIVERMLAQAGGTLIWDLGANVGTYSDIAAGPGRQVIAFDQDPAVVELHWRSLSPEQR
                     ASVLPLVNDLTNPSPALGWAHTERRSMLQRGPADVAMALALVHHLAIGNNVPLPQVAE
                     LLAHAGRRVVVEFVPKEDPMVQQLLAGRRDIFDAYSLDAFRTALDEWFVTMDEAPVTD
                     SLRTLFLLERRPD"
     gene            complement(3265..3465)
                     /locus_tag="VGO32_04245"
     CDS             complement(3265..3465)
                     /locus_tag="VGO32_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199995.1"
                     /translation="MPPIAYIDPGSGSLLLQALIAALLAVPFIFRRTIGGFWHRLRGD
                     AEPEESASAEVVSSDGPNTSDH"
     gene            3505..>4778
                     /locus_tag="VGO32_04250"
     CDS             3505..>4778
                     /locus_tag="VGO32_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199996.1"
                     /translation="MLTRERWAGILAWSYWPWHVLLLGAYPALFLFSSNLEDEVTLEP
                     LWGPLLACLGIAGGLLVGFFALRRDWARAGLMASTVLLVFFTFGHAWNLISPFLDQRW
                     LLAAIWVVWGVALLQLAWRGGAWVRPATRALNAIVLLLVLINVVTIGGYMAATHTFAS
                     MTGEVPDVHVGEAGKPDVYYIVLDRYAGETTLSRFFDYDNRPFLEELEERGFSVAHDS
                     WANYFKTSLSVFATMTMSHINPDLYDDAVPNTFAQIHKAFQARMAVPATFKSLGYEYV
                     HIANWWEPTSRNADADVIYRFTQETEFSTALYDTTLLSALSPEVSAGGSGEDGDGTAA
                     NDSEELKGLELQRAHVLLGFDSLEEATQRGGPTFTFAHLTVPHPPYAFNEDGTEPTPE
                     QQAQRTDNEGYIEQLKYANRRTLEAIDQIIANT"
BASE COUNT          732 a         1648 c         1699 g          699 t
ORIGIN      
        1 cgagctcggc ccgcaaggcc gctgcggcgt ccgcgtcctc gaccttgtcc acctcggcca
       61 ggacggcgtc caccagcccg gggatcccgg gcagtcgttc gatggccttc tcggtgtgga
      121 atcgggccac cgggcgatcg ccggggtcca ggaggttcgc acgggccgcg tcgagaacgg
      181 cgggcacgcc gcgcagccgc gaggcggcgc tggcgagccg ttccgggagc ggggcgaacg
      241 gtcgcgcgag cagcgcgaac aggccgttgc ccaggacgta gacgtagacc agcggattcc
      301 agcgctcctc ctggagcgtg gtctcaccga actccatggc cgccagcgcg tcggtcagga
      361 tccggtggtc gatcgcgtcg tcgcgatcga gcgacgcgcg gtcgatctcg cccagctgac
      421 ggagggcatc ctgggcgaac gcccgtcgga tttccgctcc ggccgggctc aggtcgggcc
      481 aggctccatc gtgctcgtgg ttgccgatct cggtggcgtg gaccggatcg agacggaaga
      541 actcggcgag caggtggtcg accagggcgc gaaagtccat cggtacctcg catgcgggga
      601 cggagccccc atggtagccg ggctatgctc ggcccatggc cgacgtcccg gaccggagcg
      661 cgtacctggc cgaggcgcgc gccatcctgg cccgccatcc gctgatcgac ggccacaacg
      721 atctcgcgtg ggagattcgc aagtaccccg ccgcgccggg cgacctggat gcctacgatc
      781 ttcgggtccg ggcccccgcg ccgggcgaca cggacatccc ccgcctgcgg gaagggggcg
      841 tgggcggcca gttctggagc gtgtacgtgc ccggcgaggg ccaggcgccg cgtgccgtcc
      901 tgcagctcga gcagctggac ctcgcgcggc ggatggtggc ccattacccc gacgacatgg
      961 ccctgtgcct gaccgccgac gacgtggacc acgccctggc cgagggccgg atcgcgtcgc
     1021 tgctcgggat ggaaggcggc cacgtgctgg aaggctcgct tggcgcgctc cgggcgttct
     1081 gggagctcgg tgcgcgctac ctgaccctca cccacaacac cgcgaacgag atcggcgact
     1141 cggggaccga tgcgccccag cacggcggcc tgtccccgtt cgggcgccag gtggtggccg
     1201 agatgaaccg gctgggcatg ctcgtcgacc tgtcgcatac ctcggtcgag accatgagcg
     1261 acgccctgga cgcaacgcag gcgccggtca tcttcagcca ctcctcggcc cgtgccctgt
     1321 gcgacgtggc gcgcaacgtc ccggacgaga tcctgcgccg gatgccggcc aacggcggcg
     1381 tggtgatggt cacgtttgtc gccggattca tcggtcccga ggcagccgcc atcctcattc
     1441 cggccatcgc cgagttcggt cgtcgcgcgg ccggaatcca ggacgtggcc gtccgcgagg
     1501 ccctcgcccg cgagatcatc gactccctga ccgtgcccag cccgccgctg gagctggtcg
     1561 cggatcacgt cgaccacgtg gcgcgagtcg cgggccacga ccacgtcggg atcggtggcg
     1621 actacgacgg caacacgttc tggccggtcg acctcgagga tgtcagctgc tacccgaacc
     1681 tgatcgccga gctcctgcgc cgaggatgga ccgaggagga cctgggcaag ctggcgaatg
     1741 gcaacatccg ccgggtcctg cgcgatgcgg aggccgccgc cggctgaccg aggggacggc
     1801 gctgaacgtc agtccgggcg gcgctcgagc aggaacaggg tccgcaggct gtcggtcacc
     1861 ggagcctcgt ccatggtcac gaaccactca tcgagcgcgg tccggaacgc gtccagcgag
     1921 taggcatcga agatgtcgcg tcggccggcc agcagctgct ggaccatggg gtcctccttg
     1981 ggaacgaact ccaccacgac ccgtcggccg gcgtgggcca ggagctcggc cacctgcggc
     2041 agcggaacgt tgttgccgat cgcgaggtgg tggacgaggg ccagggccat ggccacgtcg
     2101 gcagggccgc gctggagcat cgagcggcgc tcggtgtggg cccagcccag ggcgggactg
     2161 gggttggtca ggtcgttgac gagtggcagg accgatgccc gctgctccgg cgacaggctg
     2221 cgccagtgga gctccaccac cgcggggtcc tggtcgaagg cgatgacctg ccgaccaggc
     2281 ccggccgcga tgtcggagta cgtccccacg ttggcgccga gatcccagat cagggtgccg
     2341 cccgcctggg cgagcatccg ttccacgatc tcgcgcttcg aggcggcggc ggcttccgtg
     2401 tagctcgtct ggcgggtgta cgacagccag tggctgccca gctcgagatt gagcccctcg
     2461 accgcccggc ggaggctgtc gagcagcgcc tcgtgccggg tcctgttcat cggccgggcc
     2521 cgtgactggg tcgcacccgg ttccgaggcg ccctggatct cggcccgctg cgcacgccgg
     2581 tcggcgccgg catggagatg gagatggggc agcagcccgc cgatccgagt gcggccgggc
     2641 aggagggcgg tggtcaggtc cagggggagg ccgtcgatga agtcgcggag catgaggccg
     2701 cagcgcgggt cgcgatgggc catgagcgcc agcggggcca ggaagtgctt gcagaactgg
     2761 cggtaggcgg gccatggcgc cgagagatcc gcgacctcga acgagaggct gtcgatcagg
     2821 atcggccggc cggcgtcgac ctggacgttg tacgccgacg cgtcgcggag ccacatcccc
     2881 gccgacaggg cccgtcgctg gacctcgagg gtgaggagcg ccgcctcgcg gagctgcgag
     2941 aacgcccatt cgtatgggta ggagatgatc ggcagctcgc ggggccggat cacgagcgcc
     3001 gccccgggcc gcggcgcgag gtccaccgac accggcgcat agcccacgag cagcccggcg
     3061 ccgaccagct ccgccgccag gccccggtcc tcgaaggcgg accaatccgc cgcggccacc
     3121 ggctggatgg cgcgatacag gacgccgtcg cgacggaaga tgtagccgcc gggatcgcgg
     3181 aacgaggcag gatcgaccac gccggatgat gcttcgccgg gtgtcatccc cgcgtcaagc
     3241 gggcgccatc aggcgccatc ggtgtcagtg gtcgctggtg ttcggaccgt cggacgacac
     3301 aacttcggcc gacgcggact cctcgggttc tgcatccccg cggagtcggt gccagaatcc
     3361 gccgattgtg cgacggaaga tgaacggcac ggccagcagc gcggcgatca gcgcctgcag
     3421 gagcaggctg cctgaaccgg gatcgatgta cgcgatggga ggcatgtgcg gttgcggatc
     3481 cccgctgcgg gtactgttga gccgatgtta acccgggaac gatgggccgg catattggcc
     3541 tggtcgtatt ggccatggca cgtcctgctc ctcggcgcct accccgccct gttcctgttc
     3601 agctcgaatc tcgaggacga ggtcaccctc gagccgctct ggggcccgct gctcgcctgc
     3661 ctcggcatcg cgggcggcct gctggtcggc ttcttcgcgc tccgccggga ctgggctcgt
     3721 gccgggctga tggccagcac ggtgctcctc gtgttcttca ctttcggcca tgcatggaac
     3781 ctgatcagcc cgttccttga ccagcgctgg ctgctggcgg ccatctgggt ggtatggggt
     3841 gttgcgctcc tccagctggc gtggcgcggc ggggcgtggg tccggccagc gacccgggcc
     3901 ctcaacgcca tcgtgctcct cctggtcctc atcaacgtgg tgaccatcgg cggctacatg
     3961 gctgcaaccc acacgttcgc ctcgatgacc ggcgaggtgc cggacgtgca cgtcggcgag
     4021 gcgggaaagc cggacgtgta ctacatcgtc ctcgaccgct acgccggcga gacgacgctc
     4081 agccggttct tcgactacga caaccggccg ttcctcgagg agctggagga gcgcgggttc
     4141 tcggtcgccc atgactcgtg ggcgaactac ttcaagacct cgctctcggt tttcgcgacc
     4201 atgaccatga gccacatcaa ccccgacctg tacgacgacg cggtaccgaa cacgttcgcg
     4261 cagatccaca aggcgttcca ggcgcggatg gcggtcccgg ccaccttcaa gtcgcttggc
     4321 tacgagtacg tccacatcgc gaactggtgg gagccgacct cgcgcaacgc ggatgccgac
     4381 gtcatctacc gcttcaccca ggagaccgag ttcagcaccg ccctgtacga caccaccctg
     4441 ctcagcgcgc tgtcgccgga ggtctcggcc ggtgggagcg gcgaagacgg cgatggcacc
     4501 gccgcgaacg acagcgagga gctcaagggg ctcgaactcc agcgcgcgca cgtcctgctg
     4561 ggcttcgact cgctcgagga ggccacccag cgcggcggcc cgacgttcac cttcgcccac
     4621 ctgaccgtcc cccatccgcc gtacgcgttc aacgaggacg gaaccgagcc gacccccgag
     4681 cagcaggccc agcgaaccga caacgagggc tacatcgagc agctgaagta cgccaaccgg
     4741 cggacgctgg aggccatcga ccagatcatc gccaacac
//
LOCUS       DASYGU010000157         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1826603, whole genome shotgun sequence.
ACCESSION   DASYGU010000157 DASYGU010000000
VERSION     DASYGU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826603"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGO32_04255"
     CDS             complement(<1..239)
                     /locus_tag="VGO32_04255"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV7199997.1"
                     /translation="MRLLAADIGNSNTGLGLFEDDVLVGSWSAETDPRATDDELAATV
                     TGLLALDGHRLDDVDAAALACVVPPLVARWTRMLAR"
     gene            315..785
                     /locus_tag="VGO32_04260"
     CDS             315..785
                     /locus_tag="VGO32_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7199998.1"
                     /translation="MEPTPEPRAKLYINTGLVTDKSDIYNILRPLLKQTVTYTYFRGN
                     QVASYGTGWVERLVVDHPEIASYFTPLAICLNLDSFDYLQFDTTSDQLLTYTLVIGSE
                     KVMIEFAQTATEIGLEDEAQIRMPTTVDEIAFDPDFIQMELLKSPDTPDEDEDG"
     gene            814..2073
                     /locus_tag="VGO32_04265"
     CDS             814..2073
                     /locus_tag="VGO32_04265"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV7199999.1"
                     /translation="MLPNGLRVVSQAMPHARSVSVALFVGVGSRHEADGEAGLSHMVE
                     HLAFKGTDRYPEPGTLSEAIEACGGTVNASTDRELTVYSARVPRTRADRAFEVVAELA
                     LHPLLRPADLVAEKPVVVDEIRAYVDSPSDHVFSLFDELLFGSHPLGREIAGTPRGVR
                     RTSQASVERHWRRWYAAEHMVLAVAGAIDHAAVRRAAKAWFAPPGRPAGSRRTVRFSK
                     PSAAAPGTVRVGYRRLTQGNLCLGMPGVSRTHPDRWALDLLTAVLGDGMSSRLFLELR
                     ERRSLAYDVGTFATSYADCGTVGIHAGFDPAQADAVIRAALEQLERVVQDPIPAAEVD
                     RAKAYTRGRIELRLEESSAVAAWLGTGESLLPRIMTVDEVIEQFETVTADDMLRVART
                     YLRPNQARIALLGPFRGRRKVEALLAA"
     gene            2070..2771
                     /locus_tag="VGO32_04270"
     CDS             2070..2771
                     /locus_tag="VGO32_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200000.1"
                     /translation="MSAESGIDLVPDQLALARAQMDAAQYGVVEGTLRRRIATLEATG
                     RNPEELDSARALLAEALWRSGRPVAAYEAVTAVRPRGVERRRTITMMVEAEGLAAIGE
                     QRQAEGLQERVVTAVGADEAWRLRRGAPSRLDWPTPSWAGPSSTAIDASPAEDRLGSA
                     AVAYGTGDFATGDRELAIALRRDPRIAVSGIALIESGLGAQPSAERLVLYGDLLRAAG
                     RERDAAAAYDRAART"
     gene            2795..3262
                     /gene="ndk"
                     /locus_tag="VGO32_04275"
     CDS             2795..3262
                     /gene="ndk"
                     /locus_tag="VGO32_04275"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV7200001.1"
                     /translation="MERTLVLVKPDGVQRGLVGEIVGRLERKGLKLIGLRLLWVSREL
                     AEQHYAVHAGKHFYAGLVEFITAGPVAAVAVAGPDAVAVVRRLVGETMPNEAEPGTIR
                     GDLGVSGLRNLIHASDAVETAEAELALWFESGALVDYERAVDRWIVAEEPPAG"
     gene            complement(3208..>3810)
                     /gene="lepB"
                     /locus_tag="VGO32_04280"
     CDS             complement(3208..>3810)
                     /gene="lepB"
                     /locus_tag="VGO32_04280"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV7200002.1"
                     /translation="VAQPFEVEQNSMVSTIQPSDYVLIDKLTPHWNDYVRGDIVVFQP
                     PNGYEQGGVPFIKRVIGLPGETVRLENGAVFVTPVNGVEVRLTEGYVVTGTDGRPSPT
                     LPRDAEGTDRWVIPEGMYFVMGDNRPESQDSRFFGPIERDLIVGRAFVRYFPLDRITL
                     FKVPAYPGLDETGLRDGWSAWLDQPAGGSSATIQRSTARS"
BASE COUNT          572 a         1337 c         1348 g          553 t
ORIGIN      
        1 cgggcgagca tccgggtcca tcgggcgacg agcggcggca cgacgcaggc cagcgcggcc
       61 gcgtcgacat cgtccagccg gtggccgtcc agggccagga gcccggtgac ggtggccgcg
      121 agctcgtcgt cggtcgcgcg gggatcggtc tcggctgacc atgagccgac gagcacgtcg
      181 tcctcgaaga gcccgaggcc cgtgttcgag ttgccgatat cggccgccag gagtcgcatc
      241 cgcggagcat cgtaacagcc aacttgaccg attctggcgg tgctagactc ccccagaccc
      301 cgcaggagtg accgatggag cccacgcccg aaccgcgcgc caagctctat atcaacaccg
      361 gtctggtcac cgacaagagc gacatctaca acatccttcg tccgctgctg aagcagacgg
      421 tgacctacac ctacttccgc ggaaaccagg tggccagcta cggaacgggc tgggtcgagc
      481 ggctggtggt cgaccatccg gagatcgcgt cctacttcac gccgctcgct atctgcctga
      541 acctcgactc gttcgactac ctccagttcg acaccacctc ggatcagctc ctgacctaca
      601 ccctggtcat cggctccgag aaggtgatga tcgagttcgc gcagaccgcg accgagatcg
      661 ggctcgagga cgaggcgcag atccgcatgc cgaccaccgt cgacgaaatc gcgttcgatc
      721 cggacttcat ccagatggag ctcctcaagt cgcccgacac ccccgacgag gacgaggacg
      781 gctagacggc gcgctcccgc ccatgagcgc agcttgcttc cgaacggcct tcgcgtggtg
      841 agccaggcca tgccgcacgc ccgatcggta tcggtggccc tcttcgtcgg ggtcggcagc
      901 cggcacgagg ctgacggcga agccgggctt tcccacatgg tcgagcacct cgcattcaag
      961 ggcaccgacc gctacccgga gccggggacc ctgtccgaag ccatcgaggc ctgcggcggg
     1021 acggtgaacg cctccaccga ccgggagctg accgtctact cggcgcgcgt cccgcgcacc
     1081 cgcgccgacc gtgccttcga ggtggtggcc gagctcgccc tccacccgct cctgcggccc
     1141 gctgacctcg tcgccgagaa gccggtggtg gtggacgaga tccgcgccta cgtcgactcc
     1201 ccgtcggacc acgtcttctc gctcttcgac gagctgctgt tcggatccca tccgctcgga
     1261 cgggagatcg cgggcacgcc gcgcggcgtc cgccgcacga gccaggcgtc ggtggagcgc
     1321 cactggcggc gctggtacgc ggccgagcac atggtcctgg cggtggctgg tgcgatcgac
     1381 cacgccgccg tccgccgcgc agcgaaggcg tggttcgcgc ctcccggccg cccggccggt
     1441 tcgcgccgta cggtgcgatt cagcaagccg tcggctgccg cgccggggac cgtccgggtg
     1501 ggctaccggc gcctgaccca gggcaacctg tgcctgggaa tgcccggcgt gtcgcggacc
     1561 catccggacc gatgggcgct cgaccttctg accgcggtcc tgggtgacgg gatgagcagc
     1621 cggctgttcc ttgagctccg agagcgacgg tcgctggcgt atgacgtcgg caccttcgcg
     1681 acctcctacg ccgactgcgg cacggtcggg atccacgccg ggttcgaccc ggcccaggcc
     1741 gatgccgtga ttcgggccgc gctggagcag ctggagcggg tggtccagga cccgatcccg
     1801 gccgcagagg tggatcgcgc caaggcctac acgcggggcc gcatcgagct ccggctcgag
     1861 gaatccagcg cggtggccgc ctggctgggg accggggaga gcctcctgcc ccggatcatg
     1921 accgtggacg aggtgatcga gcagttcgag acggtcacgg ccgatgacat gctccgcgtc
     1981 gcgcgcacct acctgcgccc gaaccaggcc cgcatcgcgc tgcttgggcc cttccgcggg
     2041 cgtcgcaagg tcgaggccct gctggccgca tgagcgccga gagcgggatc gacctcgtcc
     2101 ccgaccagct ggccctcgcc cgggcccaga tggacgccgc tcagtacggg gtggtggagg
     2161 gaactcttcg ccgtcggatc gcgacccttg aggcaaccgg gcgcaacccg gaagagctgg
     2221 actcggcacg ggcactgctt gccgaggccc tgtggcgctc cggccgaccg gtggcggcgt
     2281 acgaagcggt gaccgccgtc cggccgcgcg gcgtcgagcg gcgccggacg atcacgatga
     2341 tggtcgaggc cgaggggctg gccgcgatcg gcgagcagcg gcaggccgag ggtctccagg
     2401 agcgggtcgt gaccgcggtc ggcgccgacg aagcctggcg cctgaggcgg ggagccccga
     2461 gccggctgga ctggccgacg ccgtcgtggg cggggccgtc gtccacggcc atcgatgcct
     2521 caccggccga ggaccggctg gggtccgcgg ccgtggccta tgggacaggc gacttcgcca
     2581 caggcgatcg ggagctggca atcgcgctgc gccgcgaccc gcgaatcgcc gtgtccggca
     2641 tcgcgctcat cgagtccgga ctcggcgcgc agcccagcgc ggagcgtctg gtactgtacg
     2701 gcgacctcct ccgggcggcc ggtcgcgaac gcgacgccgc tgccgcctac gatcgcgccg
     2761 cgcgcacctg atcccacctg ccaggagagc ttccatggag cgcaccctcg tcctcgtcaa
     2821 gcccgacggc gtgcagcggg ggctggtcgg ggagatcgtg ggccggctcg agcggaaggg
     2881 cctcaagctc atcggcctcc gtctgctgtg ggtctctcgc gagctcgcgg agcagcacta
     2941 cgcggtccac gccggcaagc acttctacgc cgggctggtc gagttcatca ccgcagggcc
     3001 ggtcgcggcg gtcgcggtcg ccgggccgga tgcggtcgcg gtcgtgcgac ggctggtggg
     3061 cgagacgatg cccaacgagg ccgagccggg cacgattcgc ggggacctgg gcgtgagcgg
     3121 gctccgcaac ctgattcacg cgtcggacgc ggtggaaacc gcggaagcgg agctcgccct
     3181 gtggttcgag tcgggcgcgc tcgttgacta tgagcgcgcg gtggaccgct ggatcgtggc
     3241 ggaggagccg ccggccggct gatcgagcca ggctgaccac ccgtcccgga gaccggtctc
     3301 gtccagcccc ggataggcgg ggaccttgaa cagcgtgatc cggtcgagcg ggaagtagcg
     3361 gacgaacgcc cgacccacga tgaggtcccg ttcgatcggc ccgaagaagc gcgagtcctg
     3421 gctctccggc cggttatcgc ccatgacgaa gtacatgcct tcggggatca cccagcgatc
     3481 cgtaccctcg gcgtcgcgtg gcagagtcgg cgacggccgc ccatcggtcc cggtcacgac
     3541 gtagccctcc gtcaggcgga cctcgacccc gttgacgggg gtcacgaaca cggcgccgtt
     3601 ctcgaggcga accgtctcgc ccggcaggcc gatgacccgc ttgatgaacg gaactccgcc
     3661 ctgttcgtac ccgttcggcg gctggaagac gacgatgtcg ccgcgaacgt agtcgttcca
     3721 gtgcggcgtg agcttgtcga tgagcacgta gtcactgggc tggatggtcg acaccatcga
     3781 gttctgctcg acctcgaacg gctgcgcgac
//
LOCUS       DASYGU010000158        13092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_26288, whole genome shotgun sequence.
ACCESSION   DASYGU010000158 DASYGU010000000
VERSION     DASYGU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26288"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VGO32_04285"
     CDS             <1..390
                     /locus_tag="VGO32_04285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7200003.1"
                     /translation="PEIALAHRRSPSLWALPKGTPNSGETIEETALRETAEETGLAVE
                     IEAAIGPISYAFVRGSTRYFKTVHFFLMRPVGGDLADHDHEFDEVRWVALSEALRLMN
                     YATERDVVEQVEARLAVGAEAIVDRAP"
     gene            387..1517
                     /gene="gcvT"
                     /locus_tag="VGO32_04290"
     CDS             387..1517
                     /gene="gcvT"
                     /locus_tag="VGO32_04290"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV7200004.1"
                     /translation="MTHGAAPRRTPLDAVHRALGARLIPFAGYEMPVRYTSELEEHRA
                     VRTAVGLFDLSHMGEVALRGPDALAFAAHALVSDPGRLEPGQAQYSMLCATDGGVIDD
                     LIVYRLEDRYLVVCNAANRDAVVAHLAALAAERGWGVTVEDESDETALVAPQGPAAAA
                     LMAGLTETDLGTLGNYRSVPATVAGVSCLVARTGYTGEDGFELFCAADDAERLWHAVS
                     EAGRAHGIRPCGLASRDTLRLEAGMPLYGNELGRDVNPYEANLGRVVKLDKGEFVGRD
                     ALAAIERDGPRRRLVGLVMTEEGIARHGYPVVDEAATVGAVTSGSVSPTLGTRIAMAL
                     VASDAAVDERPLAVMIRDRRCRAERVKLPFYRRPRPAEEPTT"
     gene            1514..1897
                     /gene="gcvH"
                     /locus_tag="VGO32_04295"
     CDS             1514..1897
                     /gene="gcvH"
                     /locus_tag="VGO32_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV7200005.1"
                     /translation="MMLPDDRRYSSEHEWVRPEGEEGVIGLTTFAASELGDIVYIELP
                     ALGTTLTQFASFGVVESVKAVSDLFAPAGGTVVAVNETLRTQPELMNSDPHGDGWICR
                     ITLSSPAELDALLDAAAYGNLIGLE"
     gene            1894..3237
                     /gene="gcvPA"
                     /locus_tag="VGO32_04300"
     CDS             1894..3237
                     /gene="gcvPA"
                     /locus_tag="VGO32_04300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEV7200006.1"
                     /translation="MSYSPHTDADRATMLAAIGVATVDELFADIPPSVRATRFAVPPG
                     LTEQEVRAELTRLAGRNRLPEVSFLGAGAYRRLIPAVVDEVVGRAEFATAYTPYQPEV
                     SQGTLQAIYEFQTLICELTGMEVASASHYDGATATAEAALMACRLTGRDRVAVSRAVH
                     PQVRRVLETYCSGPGIEVVEVPADLAAGGSGQTDTDAAARLADADVACLVVQQPNMLG
                     TLEDMPALAEAAHRAGGMFVTLVDPVSLAVLEAPGRYGADIVTAEGQQLGIPLSFGGP
                     YLGLMAARMDAVRQMPGRLAGATRDAEGRRGYVLTLQAREQHIRREKAASNICTNQAL
                     CALAATAYLAAVGPEGLREVAERSATQARHLVASLEAAGLAERRFAEPYLNEVTLSVP
                     GAARRHAELAAEGIVAGLVLEAEYPELSDTLLLAATELTTDDDVARLVAALASAP"
     gene            3234..4880
                     /gene="gcvPB"
                     /locus_tag="VGO32_04305"
     CDS             3234..4880
                     /gene="gcvPB"
                     /locus_tag="VGO32_04305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEV7200007.1"
                     /translation="MSAVDSRHVADASGRGAAFQSPVAGSAPRVVEPTLFERSRPGRR
                     AVRFPPPSDAAQASADSQPPVPAAARRSAPPRLPEVNELELVRHFNRLSRLNHAIDID
                     FYPLGSCTMKYNPKINEWAARLPGLAGSHPLDPAALSQGSLELQWLLAELLAEIGGFA
                     AVSLQPAAGAHGELTGMLMTRAFHRSRGEGDQRRVVIVPDSAHGTNPATATMVGFTTR
                     TVRSNAHGGIDLEALRAALGPDTAALMLTNPSTLGIFEDQIDEVLAAVHEAGAIAYMD
                     GANLNAILGRFRAGDAGFDIMHFNLHKTFSTPHGGGGPGAGPVGVSARMAPFLPAQLP
                     RLVAGDRSAVLANAWAGRPNPDARFELEAAGERPTAIGQVRTFGGNFGMHVRAYAYIR
                     ANGDAGLRQVTDDAVLAANYLRVGLADAYDLPFDRICKHEVVFSARRQKREHGVSALD
                     VAKAILDHGIHPPTIYFPLIVDEALMIEPTETETRETLDRFVDVMHDIAERVKADPDS
                     IRAAPTTTPVGRLDEATAARQPDLRWPFPDADRTPSAGNG"
     gene            4918..5511
                     /locus_tag="VGO32_04310"
     CDS             4918..5511
                     /locus_tag="VGO32_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV7200008.1"
                     /translation="MAEPVPGLVERMQSGDLQAFEEFFLTYQRPVYLTALAITRDPFL
                     AEEILQDVFVKAYKVRARLRTDVSPLPWLQRVCTNLCYSRLSRRRAPLEPLTDLIGNL
                     APDLSARPDVVVESREIVDVLQQGIDALPPKQQTAIILYYLHGYSLAEAAEIAGCHVG
                     TMKSRIHYALRALRVLLPVARQAPAPGRAGLADEPRT"
     gene            5516..6076
                     /locus_tag="VGO32_04315"
     CDS             5516..6076
                     /locus_tag="VGO32_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200009.1"
                     /translation="MSCERIAREVIDFFRFGELDARSAPHLDHLAVCVACREEVGLDR
                     ELVIQLRRALMARLDGHAASPGAWLEIRRRALQPEMPTWRSRLLPVFRLAPVGAAFVL
                     AIALLAPAGGFSPFRVAPDFTAIPLAQRYQETPVDDPVDPYEGRSWMRYVTAPPPSPP
                     ATRALAAADPGARQARAPQPVSGPAE"
     gene            6073..6894
                     /locus_tag="VGO32_04320"
     CDS             6073..6894
                     /locus_tag="VGO32_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200010.1"
                     /translation="MIPRRQARASLALAAVLLVVVGCTPSGGEPATPGPTTSAGPSGG
                     SPDPAAQTLTYTVDPRPGPVQPFRGEMLQHVHLAHGQVSMYFELRNIGEDPVTFINTL
                     YDYEPQQLYTPVVRLEWAEGGNVVYTRAGRFFPSPSIVPAGDTAAYLMGGQPIQGSGT
                     PADLIAHIKYCPTRGMDDTPGVPLAVSDLSWEVGHEGEVTVAGTIAEEQQAERNHSPM
                     IGVAFFAADGTFIGSVVSPADGEPLGPGASRPFSITGGGVSTDQIDHATAWAVIP"
     gene            7227..8876
                     /locus_tag="VGO32_04325"
     CDS             7227..8876
                     /locus_tag="VGO32_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7200011.1"
                     /translation="MWFKKTMGILAVLMLLLAACQPSASESPSASEGAGASVDLPPQV
                     LRVDLGGEPPTLDPTQATDSQSIQVLRSITHPLAYFDADLNIVDGLASWEISADGQTV
                     TFTLHDGIAYSDGQPIVAEDFVYSWKRLIDPRNAAGYSYVLSYVEGGAELLGADPEVD
                     DVDAMLDAFGVEAPDESTFVVHLAEPAAFFPYVATLWVTVPLREDMVFSEAEGYISSG
                     PMQLVSWEHNASIVLEPNKSWAGEPVSLERIEMAMIADPTASLAAYEADELDIGGVPS
                     QEVARIEDDPDLSEQILRGDILANYYFGYDLVDPNSPFARSVTLRRAFQEAVDKETMI
                     QVAFGGIGTVANSIVPPGMPGYQDTVYMPYDVEQAQADFETALGELGITAADLHLQIG
                     YNTDANHENKVEFLQQQWRDAFGIEVEAVGLEWGAYLDRLANDPFDIFRLGWGADFPH
                     PHNFLYDLAGCGSGNNNTGYCNEEADALMAQAAVTPALEDQIPLYNQAQDLIMADSPM
                     LMIRFSSRFTLVKPWVQDLVATSQDSSTGELFYSWASIAPH"
     gene            9020..9985
                     /locus_tag="VGO32_04330"
     CDS             9020..9985
                     /locus_tag="VGO32_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200012.1"
                     /translation="MLTYIIRRVLWIIPVLFFVSVITFALMHTVPGGPWAREKEIPAS
                     TQELLNDKYGLDEPVVVQYLNWIGPLHFEDGNVECCGGILAGDLGPSYKYIDRSVNEI
                     IGNGILVTMQLGILAFLLSVAVGLPLGIIAALGHNKAPDYSATFISVIGISTPTFVSS
                     ILLIVIFSVTLGWLPTNSLKWETDPTTWILPIVALSLFPIAQIARYTRASMLEVTRRD
                     YVRTAHSKGLGQRTVVIVHMIRNALIPVVTILGPILAFLITGSFIIEFIFSVPGIGRE
                     YVTSITRRDYGMIMAMTMFYAVAIVIMNLVVDVLYAYIDPRIRYS"
     gene            9995..10960
                     /locus_tag="VGO32_04335"
     CDS             9995..10960
                     /locus_tag="VGO32_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200013.1"
                     /translation="MTEQTGAIEAGLPLERRKSSGLWTDAFRRLLRNRLAIIGGILVI
                     FLFFIAAVGPLITPWTYYEQHTDEVIANRNRPLAPMQQIEGVDHLHYLGTDELGRDLL
                     SRLMDGARISVMVALVVQFVVLVIGIPVGAAAAWFGGRTDSILMRITDVVYAYPDLLF
                     IILLGVAFKETFFGRAMDGLLLVFVAIGLTSWVTMARLVRGQLLSLKETEFVEAAKAI
                     GVSDRKIVTRHLLPNATGPIIVAVTLGIPAAILAEATLAFIGIGVQAPRASWGSLVAD
                     GNSYVRSFPHLVIFPAMCIAVALISFTFLGDGLRDALDPKLKGKQ"
     gene            11097..12077
                     /locus_tag="VGO32_04340"
     CDS             11097..12077
                     /locus_tag="VGO32_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200014.1"
                     /translation="MLEVRNLSTQFFTGDGVVHAVDEVSFDVDYGETLGLVGESGCGK
                     SVTALSLVRLVPDPPGRITDGQIIFDGIDIMKLSRNEMRRLRGRKIGFIFQDPMTSLN
                     PTLPVGFQISESARTHLGLSRSAAGDRAVELLSKVGIPRARDRMGDYPHQFSGGMRQR
                     VMIAIALACDPMLLLADEPTTALDVTIQAQILDLIRSLSDEFGTAVILITHDLGIAAG
                     MCRRVNVMYAGRIVETGIVDDVFEKARHPYAWGLLDSLPRLTDVRASRLRTIEGMPPD
                     LVDPGVECRFSPRCRFAQDVCRTSEPELTERPGEGHMARCFGTEPGGWIP"
     gene            12197..>13092
                     /locus_tag="VGO32_04345"
     CDS             12197..>13092
                     /locus_tag="VGO32_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7200015.1"
                     /translation="MRVYFPIYSGLLQRRTGDVRAVDDVTFDIKQGETLGLVGESGCG
                     KSTTGRALIRLRNPTSGRVLFDGGDLMNLSREELRRARRRMQIIFQDPYSSLNPRMTV
                     ASIIGEPLDTHNLGSKSTRQDRVAELLQLVGLNPNFRNRYPHEFSGGQRQRIGVARAL
                     AVEPEFIVCDEPISALDVSIQAQVLNLLVELREKFGLTYLFIAHDLSVVRHISDRVGV
                     MYLGKIVEIGPPDLLYAGPGHPYTRALLSAVPIPDPKTERKRKRVILTGDVPSPVNPP
                     PGCRFHTRCWLYEQLGRPEVCRT"
BASE COUNT         1996 a         4690 c         4347 g         2059 t
ORIGIN      
        1 cccgagatcg cgctcgccca tcgacgcagc ccgtcgttgt gggccttgcc caagggcacg
       61 cccaacagcg gcgagacgat cgaggaaacg gcgctgcgcg agaccgccga ggagaccggt
      121 ctggcggtcg agatcgaagc cgcaatcggt ccgatcagct acgccttcgt ccgcggctcg
      181 acgcgctact tcaagaccgt ccacttcttc ctgatgcgtc cggtgggggg cgacctggcc
      241 gatcatgacc acgagttcga cgaggttcgg tgggtcgcgc tcagcgaggc gctccggctg
      301 atgaactacg cgaccgagcg ggacgtcgtc gagcaggtcg aggcgcgact tgccgtcggg
      361 gccgaggcca tcgtcgaccg agccccatga cgcacggcgc cgccccgcgg cggacgccgc
      421 tcgatgccgt gcaccgggcg ctcggtgccc gactcatccc gttcgccggc tacgagatgc
      481 cggtccgtta cacatcggag ctggaggagc atcgcgcggt ccgtaccgcg gtgggtctgt
      541 tcgacctgtc gcacatgggc gaggtcgcgc tccgggggcc ggatgcactc gcattcgcgg
      601 cccatgcgct tgtcagcgac ccgggccggc tcgagccggg gcaggcgcag tactcgatgc
      661 tgtgcgcgac cgatggcggg gtcatcgacg acctgatcgt ctatcgcctc gaggatcggt
      721 atctggtcgt gtgcaacgcc gcgaaccggg atgccgtcgt ggcccatctt gccgccctgg
      781 ctgcagagcg cgggtggggg gtcacggtgg aggacgagtc ggatgaaacg gccctcgtgg
      841 cgccccaggg ccccgcggcg gcggcgctca tggccggcct gaccgagacc gacctcggca
      901 cgctagggaa ctaccgatcc gtcccggcta cggtggccgg ggtgtcctgc ctcgtggcgc
      961 gcaccgggta caccggggag gacgggttcg agctgttctg cgccgcggac gatgccgaac
     1021 ggctatggca cgccgtgtca gaggcggggc gggcgcatgg catccgcccg tgcgggctcg
     1081 ccagccgcga cacgctccgg ctcgaagccg ggatgccgct gtacggcaac gagctgggcc
     1141 gtgacgtcaa cccgtacgag gccaacctcg gacgggtcgt gaagctcgac aagggggagt
     1201 tcgtgggccg cgacgcgctg gccgccatcg agcgcgacgg cccgcgccgc cggctggtgg
     1261 gcctggtgat gaccgaggaa ggcatcgccc gtcacggata cccggtcgtg gacgaggccg
     1321 cgaccgtggg cgcggtgacc agcggcagcg tgtcgccgac cctcggcacg cggatcgcga
     1381 tggcgctggt ggcgtccgat gcggcggtag acgagcggcc gctggcggtg atgatccgcg
     1441 accgacgctg ccgggccgag cgggtaaagt tgcccttcta ccgacgcccc cgccctgccg
     1501 aggaaccgac gacctgatgc tgccggacga ccgccgatac tcgagcgagc acgagtgggt
     1561 acgccccgag ggcgaggagg gcgtcatcgg tctgaccacc ttcgccgcgt ccgagctcgg
     1621 tgacatcgtc tacatcgagc tgccggcgct cgggaccacc ctgacccagt tcgcgagctt
     1681 cggcgtggtc gagtcggtga aggcggtgag cgacctgttc gccccggccg gtgggaccgt
     1741 ggtggcggtc aacgagaccc tccgaaccca gcccgagctc atgaacagcg acccacatgg
     1801 cgacggctgg atctgccgca tcacgctgag cagcccggcc gagctggacg ccctcctcga
     1861 cgccgcggcc tacgggaacc tgatcgggct cgaatgagct actccccgca caccgacgcc
     1921 gatcgggcga cgatgctggc ggccatcggg gtcgcgaccg tcgacgagct gttcgcggac
     1981 atcccacctt cggtccgggc cacccgcttc gcggtgccac ccggcctcac cgagcaggag
     2041 gtccgtgccg agctcacccg gctggcgggc cggaatcggc tgccggaggt ctcgttcctc
     2101 ggggcagggg cctatcgacg gctgatcccg gcagtggtgg acgaggtcgt cggtcgtgcc
     2161 gagttcgcga ccgcctacac gccctaccag cccgaggtca gccagggcac cctccaggcg
     2221 atctacgagt tccagaccct gatctgtgag ctgaccggga tggaggtggc cagcgccagc
     2281 cactacgacg gggcgacagc gaccgccgag gcggccctga tggcctgtcg gctgaccggt
     2341 cgtgaccggg tcgccgtgtc gcgcgccgtc catccgcagg ttcggcgcgt gctggagacg
     2401 tactgctcgg gaccgggcat cgaggtcgtc gaggtcccgg ccgacctggc cgccggcggc
     2461 tcgggccaga ccgataccga tgccgccgcc cgcctggccg acgcggacgt tgcctgcctg
     2521 gtcgtccagc agccgaacat gctcgggacg ctcgaggaca tgccggcgtt ggccgaggcc
     2581 gcacaccgcg cgggtggcat gttcgtgacc ctggtcgacc cggtcagcct ggccgtcctg
     2641 gaagcaccgg gccgatacgg cgcggacatc gtgaccgccg aaggccagca gctcgggatc
     2701 ccgctctcgt tcggcgggcc gtatctcggc ctcatggccg cgcggatgga cgccgttcgc
     2761 cagatgcccg gccgtctggc cggcgcgacc cgcgacgccg aggggcgccg cggctacgtc
     2821 ctgaccctcc aggcccgcga gcagcacatc cgtcgcgaga aggcggccag caacatctgc
     2881 accaaccagg cgctgtgcgc cctcgcggcg accgcctacc tggccgcggt cggtcccgag
     2941 ggcctgcgcg aggtcgccga acggtcggcg acccaggccc gccacctcgt cgccagcctc
     3001 gaggccgctg gtctcgcgga gcgccggttc gccgagccgt acctgaacga ggtcaccctg
     3061 tccgtcccgg gagcggcccg ccgccacgcc gagctggcgg ccgagggcat cgtggccggc
     3121 ctggtcctgg aggccgagta cccggagctg tccgacacgc tcctgctggc cgcgaccgag
     3181 ctgaccactg acgacgacgt tgcgcgcctc gtggccgccc tggcctccgc gccatgagcg
     3241 cggtcgattc gcgccacgtg gccgacgcgt cgggccgggg tgcggcgttc cagtctccgg
     3301 tcgccggctc ggcaccccgc gtcgtcgagc ccaccctgtt cgagcgctcg agacccggcc
     3361 gccgtgccgt tcgcttcccg ccgccatcgg acgcggctca ggcctcggcg gactcccagc
     3421 cgcccgttcc ggcggcggcg cgccgatcgg cgccgccacg cctgcccgag gtcaacgagc
     3481 tcgagctggt ccgtcatttc aaccggctga gccgcctgaa ccacgccatc gatatcgact
     3541 tctacccgct cggctcgtgc acgatgaaat acaaccccaa gatcaacgag tgggcggccc
     3601 ggctgccggg cctggccgga tcgcatccgc tcgacccggc tgccctgagc cagggcagcc
     3661 tcgagctcca atggctgctg gccgagcttc tggccgagat cggcggcttc gcggcggtca
     3721 gcctccagcc cgcggccggc gcccacgggg agctgaccgg catgctcatg acccgcgcgt
     3781 tccatcggtc ccgcggcgag ggcgaccagc gacgggtggt catcgtcccc gacagcgctc
     3841 acggcacgaa tcccgctaca gcgaccatgg tcgggttcac gacccggacc gtccgctcca
     3901 acgcgcacgg cgggatcgac ctcgaggcgc tgcgggccgc gctcggcccg gacaccgcgg
     3961 cgctgatgct caccaacccg agcacgctgg gcatcttcga ggaccagatc gacgaggtgc
     4021 tggccgcggt tcacgaggcg ggcgcgattg cctacatgga cggcgccaac ctgaacgcaa
     4081 tcctcggccg atttcgggcc ggcgacgcgg gcttcgacat catgcacttc aacctgcaca
     4141 agaccttcag caccccgcac ggcggcgggg gaccgggggc cgggccggtc ggggtcagcg
     4201 cacgcatggc gccattcctc cccgcgcagc tgccgcggct ggtcgccggg gatcggtctg
     4261 ccgtgctcgc gaatgcctgg gccggacgcc ccaacccgga cgcccggttc gagttggagg
     4321 cggcggggga gcgacccacg gccatcggcc aggtccggac gttcggcggg aacttcggca
     4381 tgcacgtccg cgcctatgcc tacatccggg ccaacggtga cgcggggctc cgccaggtga
     4441 ccgacgacgc ggtgctcgcc gccaactacc tgcgggtcgg actggccgat gcgtatgacc
     4501 tgccattcga ccgcatctgc aagcacgagg tggtgttcag cgcccgccgg cagaagcggg
     4561 agcacggggt gtccgcgctg gacgtggcca aggccatcct ggaccacggc atccatccgc
     4621 ccaccatcta cttcccgctg atcgtggacg aggcgctcat gatcgagccc accgagaccg
     4681 agacccgcga gacgctcgat cgcttcgtgg acgtcatgca cgacatagcc gaacgcgtga
     4741 aggcggatcc ggacagcatc cgggccgctc cgaccacgac gccggtgggc cgcctggacg
     4801 aggccacggc cgcccgccag ccggacctcc gctggccgtt cccggatgcc gatcgaaccc
     4861 cctccgcagg caacgggtag tatctgcacc catcggcgca ggaggcacaa tctgaacgtg
     4921 gcggagccag ttccgggcct cgttgagcgc atgcagtcgg gtgacctgca ggcattcgag
     4981 gagttcttcc tcacctacca gcggccggtc tacctgaccg cgctggccat cacccgcgac
     5041 ccgttcctgg ccgaggagat cctccaggac gtcttcgtta aggcctacaa ggtgcgcgcc
     5101 cggctccgga ccgacgtctc gcccctgccc tggctccagc gggtctgcac caacctgtgc
     5161 tatagccggc tctcgcgccg ccgggcgccg ctcgagccgc tgaccgatct catcggcaac
     5221 ctcgcgccag acctgtcggc gcggcccgac gtcgtcgtcg agtcgcggga gatcgtggac
     5281 gtcctccagc aggggatcga cgccctgccg cccaagcagc agaccgcgat catcctgtac
     5341 tacctccacg gctacagcct ggccgaagcg gccgagattg ccggctgcca cgtcgggacc
     5401 atgaagtcgc gaatccacta cgcgctgcgc gccctccgcg tcctgctccc ggttgcccgc
     5461 caggcgccgg cccccggccg ggcagggctg gccgatgagc caaggacctg agccgatgag
     5521 ctgcgagcgc atcgcgcgcg aggtcatcga cttcttccgc ttcggcgagc tcgatgctcg
     5581 ctccgcgccg cacctcgacc acctggccgt gtgcgtggca tgccgggaag aggtggggct
     5641 cgaccgcgag ctcgtcatcc agctccgacg cgcgctcatg gcccgactcg acggacatgc
     5701 cgcctcgccc ggagcctggc tcgaaatccg tcgccgcgcc ctccagcccg aaatgccgac
     5761 ctggcgcagc cggctgctgc cggtcttccg cctggcgccg gtgggggcgg ccttcgtcct
     5821 ggccatcgcg ctcctcgccc cggctggcgg cttctcgccg ttccgggtgg ccccagactt
     5881 cacggcgatc ccgctggccc agcgctacca ggaaaccccg gtggacgacc cggtcgatcc
     5941 gtacgagggc cgcagctgga tgcggtacgt gaccgctccg ccaccgtcac cgccggcgac
     6001 ccgggccctg gccgcggccg acccgggcgc gcgccaggct cgggccccgc aaccggtcag
     6061 cggccccgcc gagtgattcc ccggcgccag gcccgggcga gcctcgcgct cgccgcggtc
     6121 ctgctcgtcg ttgtcggctg cactccctcg gggggcgagc ccgccacgcc gggtccgacg
     6181 accagcgcgg gaccctccgg tggctcgccc gatccggccg cgcagaccct gacctacacc
     6241 gtggatccga gacccggtcc ggtgcagccg ttccggggtg agatgctgca gcacgtccac
     6301 ctggcccacg gccaggtgtc gatgtacttc gagctgcgca acatcggcga ggacccggtg
     6361 acgttcatca acaccctgta cgactacgag ccgcagcagc tgtacacccc tgtcgtccgt
     6421 ctcgaatggg cggagggagg caacgtggtc tacacccgcg ccggccggtt cttccccagc
     6481 ccgtccatcg ttccggccgg agacacggcc gcgtatctca tgggcggcca gccgatccag
     6541 ggttccggta cgccggccga cctcatcgcc cacatcaagt actgcccgac tcgcggcatg
     6601 gatgacaccc cgggggtgcc gctggccgta tcggacctga gctgggaggt ggggcatgag
     6661 ggcgaggtga ccgtcgccgg caccatcgcc gaggagcagc aggcggagcg caaccactcg
     6721 ccgatgatcg gcgtcgcgtt cttcgccgcc gatgggacgt tcatcggctc ggtggtgtcc
     6781 ccggccgacg gcgagccgct cggcccgggt gcttctcggc cgttctcgat caccggcggc
     6841 ggggtgagca ccgaccagat cgaccatgcg accgcgtggg ccgtcatccc ctgatctggc
     6901 gatgacgagt gaacctcgcg cccttcctgc ctgtagtcag ggtgtggaac cgggtgtccg
     6961 gtcgtcatgg tgcgcgtttg acagtttctt gacgacgacc gaggccacgg gccatgattg
     7021 ccgggcttgc cgcgggcgcc gcccaacggc gtcgtgccgg tgcgccccgg tttttgctac
     7081 gcacggttag gagaggaggg cctgccgagc gacgatcgct gaacgttgtc atgggctcga
     7141 gcgtctcgag cacgtggtga tcagatcgcc gactttcccg cggcgaatct tccgtaccta
     7201 cctccaccag gtgaaaggaa aggtgtatgt ggtttaagaa gacgatgggc atccttgcgg
     7261 tgctcatgct tctcctggcg gcgtgccagc cgtccgcgtc tgagtctccg agcgccagtg
     7321 agggcgccgg tgccagcgtg gatctgccgc cccaggttct gcgggtcgac cttggcggcg
     7381 agccgccgac gctagatccg actcaggcga ccgacagcca gtcaatccag gtgctgcggt
     7441 cgattaccca cccgctggcc tacttcgacg ctgacctgaa catcgtcgac ggccttgcca
     7501 gctgggagat cagcgccgac ggacagaccg tgactttcac cctgcacgac ggcatcgcct
     7561 atagcgacgg ccagcccatc gtggctgagg acttcgtcta tagctggaag cgcctcattg
     7621 acccgcggaa cgccgctggc tactcgtacg tcctctccta cgtggagggt ggcgccgagc
     7681 tgctcggtgc ggaccccgaa gtcgacgatg tcgacgcgat gctcgatgcc tttggcgtcg
     7741 aggcgcccga tgagtcgacc ttcgtcgttc atctggccga gccggctgcg ttcttcccgt
     7801 acgtcgccac gctgtgggtg acagttccgc tccgcgagga catggtcttc agcgaggccg
     7861 aggggtacat ctcctctggt ccgatgcagc tcgtgtcgtg ggagcacaac gcgtccatcg
     7921 tccttgagcc gaacaagagc tgggccggcg agccggtcag ccttgagcgg atcgagatgg
     7981 cgatgatcgc cgacccgacg gcgtccttgg cagcctacga ggctgacgag ctcgacatcg
     8041 gcggcgtgcc gtcgcaggaa gtcgcccgga tcgaggacga cccggatctc agcgagcaga
     8101 tcctgcgcgg tgacattctg gccaactact acttcggcta cgaccttgtt gatcccaaca
     8161 gtccgttcgc ccgctcggta accctgcggc gagcgttcca ggaggcggtc gacaaggaga
     8221 ccatgatcca ggttgccttc ggtggcatcg gaaccgttgc gaacagcatc gttccgcctg
     8281 gcatgccggg gtaccaggac acggtgtaca tgccgtatga cgttgagcag gcacaggccg
     8341 actttgagac ggccctgggc gagctcggca tcacggctgc cgacctgcac ctgcagatcg
     8401 ggtacaacac ggacgccaac cacgagaaca aggtcgagtt cctgcagcag cagtggcgcg
     8461 acgcgttcgg catcgaagtc gaggccgttg gtctcgagtg gggtgcctac ctcgaccggc
     8521 tggcgaacga tccgttcgac atcttccgtc tcgggtgggg cgcggacttc ccgcacccgc
     8581 acaacttcct gtatgacctg gctggctgcg gaagcggcaa caacaacacc gggtactgca
     8641 acgaggaagc tgatgccctc atggcccagg cggcggtaac accggctctg gaagaccaga
     8701 tcccgttgta caaccaggct caggatctga tcatggcgga ctcgccaatg ctcatgatcc
     8761 ggttcagctc tcggttcacg ctcgtcaagc cgtgggtcca ggatctggtc gccacgtcgc
     8821 aggacagcag caccggcgaa ctgttctact cctgggcatc catcgccccg cactaaatcg
     8881 cggattgatg gactgacctg aatacgcgtc gggcgggccg gacaaccggc ccgcccgacg
     8941 gccacggcgg ccgaggggta cccttcggac gacgtggccg tcggctgacg gcacggactg
     9001 agacggagag gcgcatacgg tgctgaccta catcattcgg cgggtgttgt ggatcattcc
     9061 agtgctgttc ttcgtgtcgg tcatcacctt cgccctgatg cacaccgtcc ccggtggccc
     9121 gtgggcccgg gagaaggaga tcccggcctc gacccaggag ctcctgaatg acaagtacgg
     9181 cctggacgag ccggtcgtcg tccagtacct gaactggatc ggcccgctcc acttcgagga
     9241 cggcaacgtc gaatgctgcg gcggcatcct ggccggcgac ctcggcccgt cgtacaagta
     9301 catcgaccgc tcggtgaacg agatcatcgg caacgggatt ttggtcacca tgcagctcgg
     9361 catcctggcc ttcctgctgt cggtggccgt cggcctgccg ctcgggatca tcgccgcgct
     9421 cggccacaac aaggcgcccg actactcggc caccttcatc tcggtcatcg gcatctcgac
     9481 cccgacattc gtgtcgtcca tcctcctgat cgtcatcttc agtgtgaccc tgggctggtt
     9541 gcccaccaac tcactgaagt gggagaccga tccgacgacc tggatcctgc cgatcgtcgc
     9601 cctgtctctc ttcccgattg cccagatcgc gcgctacacg cgggcctcga tgctcgaggt
     9661 cactcgccgc gactacgttc gcaccgcgca cagcaagggc ctcggccagc ggacggtggt
     9721 catcgtgcac atgatccgga acgcgctgat cccggtcgtg accatcctgg gaccgatcct
     9781 ggccttcctg atcaccggct cgttcatcat cgagttcatc ttcagcgtgc cgggcatcgg
     9841 ccgcgagtac gtgaccagca tcacccgccg cgactacggc atgatcatgg ccatgaccat
     9901 gttctacgcg gtggcgatcg tgatcatgaa cctcgtggtc gacgtcctct acgcgtacat
     9961 cgatccccgg atccggtaca gctaggccgc gaccatgacc gagcagaccg gggccatcga
    10021 ggccggactc ccgctcgaac gtcgcaagag cagcgggctg tggaccgacg ccttccgccg
    10081 attgctccgc aaccggctgg ccatcatcgg cggcatcctg gtcatcttcc tgttcttcat
    10141 cgccgcggtg gggccgttga tcacgccatg gacctactac gagcagcaca cggacgaggt
    10201 catcgccaac cgcaaccggc ctctggcccc gatgcagcag atcgagggcg tcgaccacct
    10261 tcactacctg ggcaccgatg agctcggccg cgacctcctg agccggctga tggacggcgc
    10321 ccggatcagc gtcatggtcg ccctcgtcgt ccagttcgtg gtgctcgtga tcggcatccc
    10381 ggtgggagcc gccgcggcgt ggttcggagg gcgaaccgac agcatcctga tgcggatcac
    10441 tgacgtcgtg tacgcctacc cggatctgct gttcatcatc ctgctcggcg tcgcgttcaa
    10501 ggagaccttc ttcggaaggg cgatggatgg gctcttactc gtgttcgtgg ccatcggcct
    10561 gaccagctgg gtgacgatgg cgcgcctcgt ccgcggccag ctgctcagcc tcaaggagac
    10621 cgagttcgtc gaggcggcca aggccatcgg cgtgagcgac cgcaagatcg tcacccgtca
    10681 cctgctgccc aatgccaccg gcccgatcat cgtggccgtc accctcggca tcccggccgc
    10741 catcctggcc gaggccaccc tggccttcat cggcatcggt gtgcaggccc cccgcgccag
    10801 ctggggcagc ctggtggccg atggcaactc ctacgtccgc tccttcccgc acctcgtcat
    10861 cttcccggcg atgtgcattg cagtcgctct gatcagcttc accttcctgg gagacggcct
    10921 tcgtgacgcg ctcgatccca agctgaaggg gaagcagtag gttgacggtc agcgacccaa
    10981 aggctccgcc cgacggcgca gccgtaaccg agctcgacga ctcggtcgag atctcgaccg
    11041 aagaggggcg tcgtcttgcc gagcggctgg ccgacaaccg gccccacgcc cgcaagctgc
    11101 tcgaggtccg caacctctcg acccagttct tcaccggtga cggcgtggtc cacgccgtgg
    11161 acgaggtcag cttcgacgtc gactacggcg agaccctggg cctggtcggg gagtccggct
    11221 gcggaaagag cgtgaccgcg ctgtcgctgg tccggctcgt gcccgacccg ccggggcgga
    11281 tcaccgacgg ccagatcatc ttcgacggca tcgacatcat gaagctgtcc cgcaacgaga
    11341 tgcgccggct ccgcgggcgg aagatcggct tcatcttcca ggacccgatg accagcctga
    11401 acccgacgct cccggtgggc ttccagatct cggagtcggc ccggacccat ctgggcctgt
    11461 cccggagtgc cgcgggcgac cgggcggtgg agctcctgtc caaggtcggc atcccgcgcg
    11521 cccgcgatcg gatgggggac tacccccacc agttctcggg cggcatgcgc cagcgggtga
    11581 tgatcgccat cgccctggcc tgcgacccga tgctcctcct cgccgacgag cccacgaccg
    11641 ccctcgacgt cacgatccag gcccagatcc tggacctcat ccgctccctg tccgacgagt
    11701 tcgggacggc ggttatcctc atcacccacg acctcggcat cgcggccggc atgtgccgcc
    11761 gggtgaacgt catgtacgcc ggccggatcg tcgagaccgg catcgtggac gacgtgttcg
    11821 agaaggcccg ccatccgtac gcgtggggcc tcctggactc gctgccccgg ctgaccgatg
    11881 tgcgcgccag ccggctgcgc acgatcgagg gtatgccgcc cgacctcgtc gaccccggcg
    11941 tcgagtgccg cttctctccg cgctgccggt tcgcgcagga cgtgtgccgg acatccgagc
    12001 cggagctcac cgagcgaccg ggcgagggtc acatggcgcg ctgcttcggc accgagcctg
    12061 gaggctggat cccgtgatcg acgacgcgac cgccaccaac gatgaggtca cggttccgga
    12121 cgccggctcg gccgtccggg acgcccggac cgcccaactt gcggacggca cgaggcccct
    12181 cgtccaggtc gaggacctgc gggtgtactt cccgatctac tccgggctcc tccagcgccg
    12241 gaccggcgac gtgcgggccg tggacgacgt caccttcgac atcaagcaag gcgagaccct
    12301 cggcctggtc ggcgagtcgg gatgcggcaa gagcacgacc ggccgcgccc tgatccggct
    12361 ccgaaacccg accagtggac gcgtcctgtt cgacggcggc gacctcatga acctgtcccg
    12421 cgaggagctg cgccgtgccc gccgccggat gcagatcatc ttccaggacc cctactcgtc
    12481 gctcaacccg cggatgaccg tggcctcgat catcggcgag ccgctggaca cccacaacct
    12541 ggggagcaaa agcacgcgcc aggaccgggt agcggagctt ctccagctgg tcggcctcaa
    12601 cccgaacttc cgaaaccgct acccgcacga gttctccggc ggtcagcgac aacggatcgg
    12661 cgtcgcccgc gcgctcgcgg tcgaaccgga gttcatcgtc tgcgacgagc cgatcagcgc
    12721 actcgacgtg tcgatccagg cccaggtgct caacctcctc gtcgagctgc gcgagaaatt
    12781 tgggctgacc tacctgttca tcgcccatga cctctccgtg gtccgccaca tcagcgaccg
    12841 ggtgggggtc atgtacctgg gcaagatcgt cgagatcgga ccgccggacc tgctgtacgc
    12901 cgggccgggg cacccgtaca cccgtgccct gctgtccgcg gtgccgatcc cggatcccaa
    12961 gaccgagcgg aagcgcaagc gggtcatcct gaccggcgac gtgccgtccc ccgtcaaccc
    13021 gccgccgggg tgccgcttcc atacccggtg ctggctgtac gagcagctcg gccgccccga
    13081 ggtctgccgc ac
//
LOCUS       DASYGU010000159         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1569272, whole genome shotgun sequence.
ACCESSION   DASYGU010000159 DASYGU010000000
VERSION     DASYGU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569272"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..344)
                     /locus_tag="VGO32_04350"
     CDS             complement(63..344)
                     /locus_tag="VGO32_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV7200016.1"
                     /translation="MSIIAWIVLGAIAGWLAGLLVKGDERLGVIGHILLGIAGAVVGG
                     FLAGLLFNTDPISGPFDISSIVTAVIGAIILVVGYGLVTGRGRSGEGSV"
     gene            complement(481..1242)
                     /locus_tag="VGO32_04355"
     CDS             complement(481..1242)
                     /locus_tag="VGO32_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7200017.1"
                     /translation="MSTQPLVLIADDEPRITKLVAIALNEEGFRVVTASSGEEAIAAA
                     EEYRPDIVLLDIVMPDRDGIEVMRELQQRQPVPVILLTAKGSTADKAKGLDLGADDYI
                     AKPFHPDELAARVRAVLRRAAGVAPQQGILRFDDIEIDLERRIVHKDGQLVHLSRTEW
                     LLLQHLAANAGKVVVHSELLTKVWGPEYRDDVQYLRVWISRVRRKLGSAPGDMGRIKT
                     FQGIGYLLDVEPPTEPVALDGDAPANAVADASATD"
     gene            complement(1327..1611)
                     /locus_tag="VGO32_04360"
     CDS             complement(1327..1611)
                     /locus_tag="VGO32_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200018.1"
                     /translation="MANQTPDAQDEREATPPPSTTGAPPPVVVQNTQDGGGSSGGMGV
                     VAIVALVAIVLVGIWWFGFGPGTGGGATDTPDTAPPSSAPVQSVPPASTL"
     gene            complement(1724..2038)
                     /locus_tag="VGO32_04365"
     CDS             complement(1724..2038)
                     /locus_tag="VGO32_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200019.1"
                     /translation="MVTEPVTLYVDAGLLADDRDDALLTASQLGFATVVVEDRSTVPE
                     TLAGAWYLSTDIPDAGSARWSRTIVIGPRAEPGRRAVTGLRTARDLRLAVLEVASEQA
                     LG"
     gene            complement(2032..3492)
                     /locus_tag="VGO32_04370"
     CDS             complement(2032..3492)
                     /locus_tag="VGO32_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7200020.1"
                     /translation="MERQRYRLARLPAIALAVAVPVGGVMLFVLADAPIRRDLGLLVA
                     VATVIVLAWLGGLWSGLIATLLLLVLFAGPLGLEQFVPALDDLGLFSFAVAGLVGTAI
                     VESLYRARWRVEVARDRARTSRRAEHAIRAELESIVAAIGDGIMVVDADDTIALMNGA
                     ATEIVGHRLNRFADLAMVLAPSANGTNLADQLSHDGPMTAEFQLMDQDRRVELSSFPL
                     LTEGEANRRVILLRDVTEARRRELLRDAFLSLLSHELRTPMTAVYGGATLLQRMGDRL
                     DAATRNDLRADIVFEAERLSRLIDDLLVLTKVEGGVEVGQEPALLQHLVADVAAQDQK
                     RAKGSSITVTADAGLSPVTGDETSIRQVTHNLISNAIKYSPPGSPVDVLVAAGDDEVA
                     VRVLDRGRGLSEREAARVFEPFYRGAETAQMATGVGIGLFVCHRLVEAMGGRIWTRPR
                     EEGGSEFGFALPVWPMDADDEFPLVPVEAGRAGPTW"
     gene            complement(3624..3893)
                     /locus_tag="VGO32_04375"
     CDS             complement(3624..3893)
                     /locus_tag="VGO32_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV7200021.1"
                     /translation="MSIFAWIVLGAIAGYLAGFLVRGDEGMGVIGHVLLGIVGALIGG
                     FLAGALLGTSPIDGALDVSSIVTATIGAILAVWAVNFLRRGSARV"
     gene            complement(4028..4447)
                     /locus_tag="VGO32_04380"
     CDS             complement(4028..4447)
                     /locus_tag="VGO32_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200022.1"
                     /translation="MDILFDALNPTNFPNMFTLIWVGAIVICIGASIVYWIAQRRYRR
                     YPAHLAMHEWVYWTILVPWIMVPLLAVIHVSLFIVLAIVIPGMAAAAFGAFIRYPPRV
                     AAANNELRRHRYVPPPRRDTRVRAKPTPAGGRKTHKR"
     gene            complement(4529..>5029)
                     /locus_tag="VGO32_04385"
     CDS             complement(4529..>5029)
                     /locus_tag="VGO32_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200023.1"
                     /translation="VGAWDGAGLDFPSVVHVDDGYLMLYAGSSLSAPNEGRLGVATST
                     DGVAWTKQPDPVVEPGLCGEFDSRSIAIPRLRQVDDGYLLFYNGLAPDLSTAEVGVAA
                     SPEGFSWTCANPMPALVAEDIPDSQGIHVIAVAAEARGPEFLVESLGDESSSVWLGDV
                     SMTGLP"
BASE COUNT          862 a         1724 c         1694 g          749 t
ORIGIN      
        1 gctcaccgta cggtgagcgc gtttcggggt cagggccgat caatcagacc gagcctcgtc
       61 aatcagacgg agccttcgcc ggacctccca cggcccgtca cgaggccgta gccgaccacg
      121 aggatgatcg ccccgatgac cgccgtcacg atcgagctga tgtcgaacgg gccgctgatc
      181 gggtccgtat tgaacaggag cccggccagg aaccctccga cgactgcgcc ggcgattcca
      241 agaaggatgt gaccgatgac gccaaggcgc tcgtcaccct tcaccagcag gccggccagc
      301 cagcctgcga tcgcgccgag cacgatccac gcaatgatgc tcacagtttc ctccaccatt
      361 ccattcccaa aacgttcacc cgatgtgggt gattggagta actgtcctgc ccaggcggat
      421 ccgtccgcct ctgtaacaag gccaacccgt attgcaaatg gctgacagtc gtcgcggagg
      481 tcagtcggtg gcgctggcgt cggctaccgc gttggctggc gcgtcgccat ccagcgcaac
      541 cggctcggtg ggcggttcga cgtcgagcag gtagccgatt ccctggaacg tcttgatccg
      601 tcccatgtca cccggggcgg agccgagctt gcgacggacc cggctgatcc acacgcggag
      661 gtactggacg tcgtcgcggt actccggtcc ccagaccttg gtcagcagct cgctgtggac
      721 gaccaccttg cccgcgttgg cggcgaggtg ctggagcagg agccactcgg tccgcgacag
      781 gtgaaccagc tggccgtcct tgtggacgat ccgccgttcg aggtcgatct cgatgtcgtc
      841 gaatcggagg attccctgct ggggagccac gccggccgcc cgccgcagga ccgcgcgcac
      901 acgggcggcc agctcgtcgg ggtggaacgg cttggcgatg tagtcgtcgg ccccaaggtc
      961 caggcccttc gccttgtctg cggtcgaacc cttggccgtg agcaggatga ccgggaccgg
     1021 ttgtcgctgc tgcagctcgc gcatgacctc gatgccgtct cggtccggca tgacgatgtc
     1081 caggagcacg atgtcgggcc gatattcctc ggcggccgcg atcgcctctt ccccgctgga
     1141 cgcggtcacg acacggaagc cctcttcatt cagggcgatc gcgaccagct tcgtgatccg
     1201 cggctcgtca tccgcaatca ggaccagggg ttgcgtgctc acggcacctc cgtggtcgaa
     1261 cctaccagat agggaaagac cgctgcgacc ccccgtcctg ggtcgcagcg gtatgaatac
     1321 gcccggtcag agcgtggacg cgggcggtac ggactggact ggcgcactgg acggcggcgc
     1381 cgtgtccggg gtatcggtcg cgcctccacc ggtacccgga ccgaagccga accaccagat
     1441 cccgaccagc acgatggcga ccaacgccac gatggcaacg acgcccatgc ccccactgct
     1501 accaccacca tcctgggtgt tctgcaccac gaccggcggc ggggcacccg tggtcgaggg
     1561 aggtggggtc gcctcgcgct cgtcctgtgc atcaggagtc tggttggcca ttttcggtct
     1621 ccaagtcatt accttcgacc cggagatcgt cgctcccgcg atgtcgcagc cgcgcccgca
     1681 cgggtcgaac aaagacattg cagcggcatg acagtccgcc ccgtcagccg agggcctgct
     1741 ccgacgcgac ctcgaggacc gcgagccgga gatcacgggc ggtccgtagg ccggtcaccg
     1801 ctcgtcgacc cggctctgcc cgcgggccga tgacaatcgt ccgagaccaa cgagcggaac
     1861 cggcgtccgg gatatcggtc gagaggtacc acgcgccggc gagggtctca ggcacggtgg
     1921 accgatcctc gacgaccacc gtggcgaatc cgagctggga cgccgtgagg agcgcatcgt
     1981 cgcgatcgtc ggcgaggagt ccggcgtcga catagagcgt gaccggctcc gtcaccatgt
     2041 cggcccggcc cgaccggctt cgacgggaac cagcgggaac tcgtcgtcgg cgtccatcgg
     2101 ccagacggga agggcgaagc cgaactcgga acctccttcc tcccgagggc gcgtccagat
     2161 ccggcccccc atggcctcga ccagccgatg gcagacgaac aggccgatgc ccacgccagt
     2221 cgccatctgg gcggtctcgg cccctcggta aaaaggctcg aacacccggg cggcctcccg
     2281 ctccgacagg cctcggccgc ggtcgaggac ccggaccgcg acctcgtcgt cgccggctgc
     2341 gaccaggacg tcgaccggcg accccggcgg gctgtacttg atcgcgttcg agatgaggtt
     2401 gtgggtcacc tggcgaatgc tcgtctcgtc gccggtcacc ggagacaggc cggcgtcggc
     2461 cgtgactgtg atggacgacc ccttcgccct cttctggtcc tgggcggcca catccgcgac
     2521 caggtgctgg aggagcgcgg gctcctgacc gacctcgaca ccaccctcga ccttggtgag
     2581 caccagcagg tcgtcgatga ggcgggagag gcgttcggcc tcgaacacga tgtccgcccg
     2641 aaggtcattc cgggttgccg cgtccagccg gtcgcccatc cgttgcagga gggtcgcccc
     2701 gccatacacg gccgtcatcg gtgtccggag ctcatgcgac agcaggctga gaaacgcgtc
     2761 ccgcagcagc tcccgccgac gagcctccgt cacgtcgcgc aggaggatga cgcgacggtt
     2821 ggcctcgccc tcggtcagca acgggaacga cgacagctcg acgcggcgat cctggtccat
     2881 gagctggaac tcggcggtca tcggtccgtc gtgagagagc tgatcggcaa ggttcgttcc
     2941 attcgcggac ggcgccaata ccatggccag gtccgcgaaa cggttcaggc ggtgccccac
     3001 gatctcggtc gccgccccgt tcatgagcgc gatcgtgtcg tcggcatcca ccaccatgat
     3061 tccgtcgccg atcgcggcca cgatggactc gagctccgcc cggatggcgt gctccgcccg
     3121 cctggatgtg cgcgcgcggt cccgggcgac ctcgacacgc catcgcgcgc gatagagcga
     3181 ctcgacgatg gccgtgccca ccaggccggc gaccgcgaag ctgaaaaggc ccaggtcatc
     3241 gagcgcggga acgaactgtt cgagtccaag cgggccggca aacagcacga ggagcaggag
     3301 agtcgcgatg aggcccgacc acagcccgcc aagccaggcc agcacgatga ccgtggcgac
     3361 ggcgaccagg agcccgaggt cgcgccgaat cggcgcgtcg gcaaggacga acagcatgac
     3421 gccgcccacg gggacggcga cagccagggc aatggccggc aagcgggcca gccgatatct
     3481 ctggcgctcc acgcgcggag tgtgaacgga gttggccggt tcgtcgatca ccaccgcgct
     3541 tcgtcctccc gactcgccgg acacgtggaa cgccggcagg gagggtcccc tgccggcgtg
     3601 tcaattccga ggtgtgtcga tcgctaaacg cgagcgctgc cgcggcgcag gaagttcact
     3661 gcccagacgg ccaggatcgc gccgatcgtc gccgtcacga tggacgacac gtcgagcgcg
     3721 ccatcaatcg gacttgtacc cagcagtgcc cctgccagga agccgccgat gagggcaccg
     3781 acgatgccga gcagaacgtg cccgatgacg cccattccct cgtcgccgcg gacaaggaag
     3841 ccggccaggt agccggcgat cgccccgagg acgatccacg caaaaatgct catgactgcc
     3901 tccattccct actggaggcc agtttccgcg ccgaggcgct gcgagactgc ctggggaccc
     3961 ggaggattcc gttgcatttc cgtcgcaacg agtccgcgca cggatgcgca ggggccgggg
     4021 cgaggcgcta gcgcttgtgg gtcttgcgcc cgccggccgg tgtgggcttg gctcggaccc
     4081 gcgtgtcacg ccgaggcggc gggacatacc gatgccggcg cagctcgttg ttggccgccg
     4141 ccacgcgcgg ggggtaccgg atgaatgcgc cgaacgcggc ggccgccatg ccggggatga
     4201 cgatggccag cacgatgaac agcgagacgt ggatgaccgc caggagcggg accatgatcc
     4261 agggcacgag gatggtccag tacacccatt catgcatggc caggtgggcc ggatagcggc
     4321 ggtatcgccg ctgcgcgatc cagtacacga tcgacgcgcc gatgcagatc acgatcgcgc
     4381 ccacccagat cagggtgaac atgttcggga agttggtcgg gttcagggcg tcgaacagga
     4441 tgtccatcgg cgggcgagtg taccgaatga cctggccggc gttaagggcc tgcctccggg
     4501 ccggcccttc gaggtatccc ggtgttagct atgggaggcc ggtcatcgag acgtcgccga
     4561 gccacaccga cgacgactcg tcccccagcg actcgaccag gaactccgga ccgcgggcct
     4621 cggccgcgac cgcgatgacg tggatgccct ggctgtccgg gatgtcctcg gcgacgagcg
     4681 ccggcatcgg gttggcgcac gtccagctga acccttcggg gctggcggcc acgccgacct
     4741 ccgccgtcga caggtccggt gccaggccgt tgtagaagag gaggtagccg tcatcgacct
     4801 ggcggagccg agggatggca atcgagcggc tgtcgaactc gccacagagt cccggctcga
     4861 cgaccgggtc cggctgcttg gtccaggcca cgccatcagt cgacgtggca acccccagcc
     4921 gtccctcatt cggagcgctg agactggagc cggcatagag catgaggtag ccgtcgtcga
     4981 cgtggacgac cgacgggaag tcgaggcccg ctccgtccca cgcgccgac
//
LOCUS       DASYGU010000160         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_231926, whole genome shotgun sequence.
ACCESSION   DASYGU010000160 DASYGU010000000
VERSION     DASYGU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231926"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..555
                     /locus_tag="VGO32_04390"
     CDS             85..555
                     /locus_tag="VGO32_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7200024.1"
                     /translation="MRHGKAGHGDPSDPNDDARQVTETGAAELRSAAALWRRLNLRPD
                     VVLSSPLPRARQTADLFVEGVGLKREPIEDDRLRPGADWSDLAQAMADHRDARRVMFV
                     SHEPDLSEAVCLLTGARAVRMRKGGLACVEFPGIPEPGAGELAWLLDPDLYSAE"
     gene            552..1013
                     /locus_tag="VGO32_04395"
     CDS             552..1013
                     /locus_tag="VGO32_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7200025.1"
                     /translation="MSAPESGRQVTRVSAYALCVENDRVLLCRIAPGPWTAVGSWTLP
                     GGGLDFGESPRVGALRELTEETGLEGEIERLAEVLDWSDQWVNPDDDVVEAFHAIQIV
                     YRVRITGGSLRNELDGSTDVAAWFTRAEAEALPLVELAQAGIRLGFGEGPP"
     gene            complement(1010..2050)
                     /locus_tag="VGO32_04400"
     CDS             complement(1010..2050)
                     /locus_tag="VGO32_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7200026.1"
                     /translation="MKAVIFRRRGGPEVLSWEDVADPVPAPDEVLVRVGACGLNHLDL
                     HVREGSNGMRAPLPHIGGLEPAGTIAAVGSAVTEWSVGDRVIVLAFTADGTCSFCLDG
                     RENLCENREIIGLTRDGGFAEYLVAPASCLVRTPDALTDAEAAALPSAFGTAWHMLVT
                     RAQTKADEWVLVLAAGSGIGSAAIQIARELGCTVIATAGSEEKLSKARELGAAHVINH
                     AATPRFELEVMKITEGRGVDVVFEHIGPATWRHSLASLRTGGRVVTCGGLTGRMAETD
                     LWNLFWRQFTIYGSMGATRSDMAGVLELVESGRVEPVIDRTFPLAETRAAQEHLDARR
                     AFGKVVVVAEPA"
     gene            2079..3332
                     /gene="fabF"
                     /locus_tag="VGO32_04405"
     CDS             2079..3332
                     /gene="fabF"
                     /locus_tag="VGO32_04405"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV7200027.1"
                     /translation="MPATPDTARVVVTGMGAITPLGNDVETLWRELLAGTSVARHVDR
                     FDSGGFRTRFAAEVVDFDPTVYMDPKDARRADRVIQFAMATSTQALAQANFHPEGDLA
                     EEVGVIVGTGIGGLTSLDDAARVMRDEGPDRVTPLTAVNMLPDMASGQVAIQFGLTGV
                     NFCVISACATGTNAVGEAAEIIKRGDALAMVVGGTEAAVTPLALAAFHRTGSLSTRND
                     DPAHASRPFDKERDGFVVGEGAGGLVLERLDFALERGATPLAEVIGYGNSADAYHVSA
                     PEPEGKGAARSMSRAMKKAGIGPADIDYINAHGTSTQLNDRSETMAIKRALGDDAYRI
                     PVSSTKSMTGHMLGAAGVVEAVVCVKAINHGIVPPTINYEFPDPDCDLDYVPNVAREM
                     PVRTAMSNSFGFGGHNATIIVRAYP"
BASE COUNT          536 a         1245 c         1179 g          508 t
ORIGIN      
        1 cggtgatctg gccggccaga ctcaatcatg ggttcccgtc gaccccgaca agccggaacc
       61 gacccagacg tgatcctcta cttcatgcgg cacggcaagg ccggccacgg agacccgtcc
      121 gacccgaatg acgatgcccg ccaggtgacc gagaccggcg cagccgagct ccgatcggcg
      181 gcagcgctct ggcgacggct gaatctgcgg cccgacgtcg tcctgtcctc gccgctcccc
      241 cgggcacgcc agaccgcgga cctcttcgtc gagggcgttg ggctcaagcg cgaaccgatc
      301 gaggatgacc gccttcgacc cggcgccgac tggtcggacc tggcccaggc catggccgat
      361 caccgcgatg cacgccgagt gatgttcgtg agccacgagc cggacctgtc ggaggcggtg
      421 tgcctcctca ccggcgcgcg tgcggtccgg atgcggaagg gcggcctggc ctgcgtcgag
      481 ttcccgggga ttcccgagcc gggcgccggg gagctggcct ggctgctcga cccggacctg
      541 tacagcgcgg agtgagcgcg cccgaatctg gccgccaggt cacgcgggtc tcggcctacg
      601 ccctctgcgt ggagaacgac cgcgttctgc tgtgccggat cgccccaggc ccatggaccg
      661 ccgtgggctc ctggacgctc cctggtggcg gcctcgactt cggcgagtcg ccgcgcgtcg
      721 gcgccctccg ggagctgacg gaagagaccg gcctcgaggg tgagatcgag cgcctcgccg
      781 aggtccttga ctggtccgat cagtgggtaa acccggacga cgacgtcgtc gaggcgttcc
      841 atgccatcca gatcgtgtac cgggtccgaa tcaccggcgg ctcgctcagg aacgagctgg
      901 acgggtccac cgacgtcgcc gcgtggttca cccgcgcgga ggctgaggcg ctccccctcg
      961 tcgagctcgc ccaggcgggc atccgcctgg gcttcgggga gggaccgccc taggccggtt
     1021 cggcgacgac gaccaccttg ccaaaggcgc ggcgggcgtc gaggtgctcc tgtgcggccc
     1081 gcgtctcggc cagtgggaag gtgcggtcga tgactggctc cacgcgcccg ctctccacaa
     1141 gctccaggac gccggccatg tcggatcggg tcgcgcccat cgatccgtag atcgtgaact
     1201 ggcgccagaa caggttccac aaatcggtct cggccatccg accggtgagc cctccgcagg
     1261 tcacgacccg cccgcccgtg cgcaggctgg cgaggctgtg gcgccaggtc gccggaccga
     1321 tgtgctcgaa cacgacatcc accccgcgac cctcggtgat cttcatcacc tccagctcaa
     1381 agcgcggcgt ggcggcatgg ttgatgacgt gcgcggcccc cagctcgcgg gccttggaca
     1441 gcttctcctc cgagcccgcg gtcgcgatca ccgtgcaccc cagctcgcgg gcgatctgga
     1501 tcgcggcgct gccgatgccc gatcccgcgg ccaggaccag gacccactcg tcagccttgg
     1561 tctgggcacg ggtgacgagc atgtgccatg cagttccgaa cgccgatggc agggcggcgg
     1621 cctccgcatc ggtcagcgcg tccggcgttc ggaccaggca ggaggccggc gcgaccaggt
     1681 attcggcgaa cccgccgtcg cgggtcaggc cgatgatctc ccggttctcg cacaggttct
     1741 cgcggccgtc gaggcagaac gagcacgtgc cgtcggccgt gaaggccagg acgatgaccc
     1801 ggtcccccac cgaccactcg gtcaccgccg atccgaccgc ggcgatcgtt ccggcaggct
     1861 cgagcccgcc gatgtgcggc agcggggcac gcatcccatt gctcccctcc cggacgtgga
     1921 ggtcgaggtg gttcaggccg cacgcgccga cccgcacgag cacctcgtcg ggcgccggaa
     1981 cgggatcagc cacatcctcc caggacagga cttcgggtcc gcctcgtcgg cggaagatga
     2041 ctgccttcat cggtctcgtt ccataatcac gcccgagtat gccagccacg cctgacaccg
     2101 cccgggtcgt cgtcaccgga atgggcgcca tcacgcccct cggcaacgac gtcgagaccc
     2161 tgtggcgcga gctgctggcc gggacgtcgg tcgcacgcca cgtggatcgg ttcgactcag
     2221 gtgggttccg aacacgcttt gccgccgagg tcgtcgactt cgacccgacc gtctacatgg
     2281 atcccaagga cgcgcggcgc gccgatcggg tcatccagtt cgcgatggcc acctcgaccc
     2341 aggcactggc ccaggccaac ttccatccgg aaggtgacct tgccgaggag gtgggggtca
     2401 tcgtgggcac cggcatcggc gggctgacca gcctggacga cgccgcgcga gtcatgcgcg
     2461 acgagggccc cgaccgggtc acgccgttga ctgcggtcaa catgctgccg gacatggcat
     2521 ccggccaggt ggcgatccag ttcgggctga ccggcgtcaa cttctgcgtc atctccgcct
     2581 gcgcgaccgg caccaacgcg gtgggcgaag ccgcggagat catcaagcgc ggcgacgcgc
     2641 tggccatggt cgtgggcggc acggaggccg cggtcacgcc gctggccctt gccgcgttcc
     2701 accgcacagg gtcgctgtcg acgcgcaacg acgacccggc ccacgcctcg cggccgttcg
     2761 acaaagagcg tgacgggttc gtggtgggcg aaggcgccgg cgggcttgtg ctcgagcggc
     2821 tcgacttcgc cctcgagcgc ggcgccacgc cgctggccga ggtgatcggc tatggcaact
     2881 cggccgacgc gtaccacgtc agcgcgcccg agccggaggg aaagggcgcc gcccgctcga
     2941 tgtcacgggc catgaagaag gccggcatcg ggcccgccga catcgactac atcaacgccc
     3001 acgggacgag cacccagctg aacgaccgga gcgagacgat ggccatcaag cgggcgctgg
     3061 gtgacgacgc ctatcggatc ccggtcagct cgaccaagtc catgaccggg cacatgctcg
     3121 gggcggccgg ggtggtggag gccgtcgtct gtgtcaaggc gatcaaccac ggaatcgtgc
     3181 cgcccaccat caactacgag ttcccggatc ccgactgcga cctcgactac gtgcccaacg
     3241 tcgcgcgcga gatgccggtc cggacggcga tgtccaactc gttcggcttc ggcggccata
     3301 acgcgacgat catcgtgcgc gcctacccct gaaccgcgac cggctgggcg ggagccgcta
     3361 ctccgtctcc gctggtgctt cgcccattcc gatgccgagc gcatggaatc cgccgtcgac
     3421 gtggatcatc tccccggtcg tgagcgatgc ccagtcgctc atcaagaa
//
LOCUS       DASYGU010000161         8123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1672723, whole genome shotgun sequence.
ACCESSION   DASYGU010000161 DASYGU010000000
VERSION     DASYGU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672723"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGO32_04410"
     CDS             complement(<1..338)
                     /locus_tag="VGO32_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200028.1"
                     /translation="MRLWSEVPRRRSQEVVADLATAVWVLLWASLGVQLYAALAQLAS
                     VGTSIGDAGEGFESAGSSLEAILSQIPLVGEGAGNIVRDAFQEIGSPLVDAGADLERL
                     LLIIAGVLGLL"
     gene            complement(377..1975)
                     /locus_tag="VGO32_04415"
     CDS             complement(377..1975)
                     /locus_tag="VGO32_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7200029.1"
                     /translation="MAVLRSAVDPASETFAANAGAMRELVADLHERTARVTSAGAGGD
                     ERAIARHRERGKLLVRERVDQLIDPGTAFLELSTLAANGLYDDDAPGAGMVTGIGRVE
                     GVECVIVANDATVKGGTYYPMTVKKHLRAQEIALENRLPCLYLVDSGGAFLPLQDDVF
                     PDREHFGRIFFNQARLSSRGIPQIAIVMGHSTAGGAYVPAMSDETVIVRGTGAIFIGG
                     PPLVKAATGEEVSAEDLGGAEVHTVTSGVADHYALTDEHALAIGRSIVRNLAWRKPEP
                     PWEPAAARPPSVNPDELYGVIPADPRVAYDVHEVIARLVDDSEFHEFKERYGETLVCG
                     FARLEGYPVGILANNGVLFSASALKGAHFIELASQRKIPLVFLQNITGFMVGREYEAG
                     GLAKDGAKLVTAVSTTAVPKFTVIIGGSFGAGNYGMAGRAYSPRQLWMWPNARISVMG
                     GPQAARVLSTVRGGFETDADRDAFEAPILAEYERQGSPYYSTARLWDDGVIDPMDTRR
                     VLAMGISAAVNDVIPESRFGVFRM"
     gene            complement(2016..2573)
                     /locus_tag="VGO32_04420"
     CDS             complement(2016..2573)
                     /locus_tag="VGO32_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200030.1"
                     /translation="MTFWNWRRGSGGSATSGSAALANGTDLAPIELYTPDTRVVGWIA
                     SNGQRVTDLLVASDELRLWQPSAGGDDMTVRPMLDKSGEWRTVPTEDVVVVMPPEWRT
                     NRQLRLHRRIQRVAADTGTFTVTGNLHITPGAPFPEPYQMHGFIPLTEAHLLHHGEPR
                     FEHVVSVVIVNTKYVETLVPLVSLA"
     gene            2709..3065
                     /locus_tag="VGO32_04425"
     CDS             2709..3065
                     /locus_tag="VGO32_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEV7200031.1"
                     /translation="MAEGAPPETTVKRFLEVDMRVGRIVSAEPFPAARNPSYRLRIDF
                     GPDIGERESAAQLTVTYPDPATLIGRQVVAVVNFPPRRIAGFASQVLVLGAMGEGGHV
                     FLLEPDPASPEGLRIG"
     gene            3104..3535
                     /locus_tag="VGO32_04430"
     CDS             3104..3535
                     /locus_tag="VGO32_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200032.1"
                     /translation="MTIANQPLLGDIPDGMPDPHVAPPQVARAGDPFASLRIVVGLSR
                     LSRNLTHQLRDVVTALNAAYIDWSFTEDVVLSELVQLQSNWSISFHGEDRIVLGMSER
                     GRTILIVDSTKMTPFFLSQAQAQAEACDAALREFAIGDGNY"
     gene            3553..4023
                     /locus_tag="VGO32_04435"
     CDS             3553..4023
                     /locus_tag="VGO32_04435"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV7200033.1"
                     /translation="MEPKPVTCTATLTGEKGEYYFIVPVDFQAVFGRHRPPVRVTIRG
                     HTFRTTPARYGSKYFVVVNRANREAMGGLDHGDRVGIRIELDTEPRVVKPPPELTATL
                     AANAEAAAVYTQLSFSHQKAYADWVAEAKRPETRVRRAGESARRLLAGRKEPSG"
     gene            complement(4165..5085)
                     /gene="thyX"
                     /locus_tag="VGO32_04440"
     CDS             complement(4165..5085)
                     /gene="thyX"
                     /locus_tag="VGO32_04440"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEV7200034.1"
                     /translation="MPPTSPNLSAKLIFPTAEIVEEYYLPIIYTACRTCYSELLPDVI
                     WDKAVSRSVADEKQQALVRKVMESGHGSTIEHVNFTFAISGVTRTLSHQLVRHRAGTA
                     FDQQSQRYVSFKGRDNYTIPDSITKGDLPDDLAGRFQRAVDENLDLYGQLLQAEVPAE
                     DARFIFPNAMQTNLIMTVNLRQLIHMSGLRLCTMAQWEIRQLFKQIRHEIFRVSPFFG
                     SFLAPKCVPLGYCDEMGNRDEHCRIRPHRDTVMAVWEAYRGGELAETDGPIVPESSPF
                     RPKPRRTDKVAVAMEAPEAAVGQAAQADRF"
     gene            complement(5668..7167)
                     /locus_tag="VGO32_04445"
     CDS             complement(5668..7167)
                     /locus_tag="VGO32_04445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7200035.1"
                     /translation="MLRSELTAAIARAVGTGLALALPAAAVAALRLFPPLTLDPAGET
                     GWLGPAALGLVALCSAISVVPLMGGLVTSGRLATGSAGLSATALTAGTAALALSGPEG
                     SASLPNSGLALTLVVVAVGFAVAAWAGYRTIADERSRWMGVILALVLAETALAAALLA
                     PGSALDSITPMLFMGAAVVAAASAAVWFRRGARLIALAPVTVALAAVLLTWSRTGTVE
                     AVIALTPLLLAPPLLVAGFAIRAQVLEVVSGLARDSESAPVIEVAAAATPLPPADDPE
                     RERIGRELRAALIELTDARHTIALQRTELERAAEADALTGVLSRSAITDRLRDEVASA
                     RRYPHSVSVVLMDVDGMGEINARHGTAIGDDVLRELALRIRVRVREADALGRMAGDSF
                     LAVLPHTDEQGATVFAEAIRDRATQRPISTGRGEVTVTISIGVTTMRSRQELSADTLL
                     ARADEAVASARAGGGNVIAYDRLHGLARLDERRRETPTDEEPLEDNGEA"
     gene            7307..7660
                     /locus_tag="VGO32_04450"
     CDS             7307..7660
                     /locus_tag="VGO32_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200036.1"
                     /translation="MQTEPVMTRSAFLAATDALIVEGDRLAVTPDWDGFRAWLLDSDQ
                     LLEQVWGRMDRYHLAWLNVGRDSAPPGSPLDEAGTARFVTEVASAKVAVLRTMRTAVE
                     RQGWRRLSDDPVESP"
     gene            7657..>8123
                     /locus_tag="VGO32_04455"
     CDS             7657..>8123
                     /locus_tag="VGO32_04455"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV7200037.1"
                     /translation="MTYDRTGGMAVILAHPADEAFTCAGSLALAHDAGETTRLLIVTR
                     GEAGNPERTPDADLAAVRESEMVRAAEAIGLNEVTLLEGYPDGAVAAEPTERLIQELF
                     AWLGNRRPDVLVTFGSHGVTGDPDHVTVGSAARWAVERLARQGVAPNSLYVIAP"
BASE COUNT         1282 a         2808 c         2811 g         1222 t
ORIGIN      
        1 agcaggccga gcacgcccgc gatgatcagg agcagccgct cgaggtccgc gccggcatcc
       61 accagcggac tgccgatttc ctggaacgcg tcgcgcacga tattccccgc cccttccccc
      121 accaacggga tctgggacag gatggcctcc aggctgctgc ccgcggactc gaagccctcc
      181 ccggcgtcgc caatgctcgt ccccaccgac gccagctgag ccaatgcggc gtacagctga
      241 accccgaggg acgcccacag caggacccag accgcggtgg cgagatccgc gacgacctcc
      301 tgcgaccggc gcctcggaac ttcgctccat agcctcatca gttccagcct agtcccgcgc
      361 cgtcgggcct gcgcgcctac atccgaaaca cgccgaagcg agactccgga atgacgtcgt
      421 tcacggccgc gctgatcccc atcgccagca cccggcgggt gtccatcgga tcgatcaccc
      481 cgtcatccca gagccgggcc gtggagtagt agggcgagcc ctggcgctcg tactcggcca
      541 ggatcggggc ctcgaaggcg tcgcggtcgg catcggtctc gaagccgccg cggacggtgg
      601 ataggacgcg ggccgcctgg ggaccaccca tgacgctgat ccgggcgttc ggccacatcc
      661 acagctgacg ggggctgtat gcacgccccg ccatgccgta gttccccgcc ccgaacgacc
      721 cgccgatgat caccgtgaac ttgggcacgg cagtcgtcga gaccgcggtc accagcttgg
      781 ccccgtcctt ggccagcccg ccggcctcgt actcacgccc gaccatgaag ccggtgatgt
      841 tctggaggaa cacgagcggg atcttccgct gggaggcgag ctcgatgaaa tgggcgccct
      901 tcaacgctga ggcgctgaac aggaccccgt tgttggccag gatcccgacc gggtagccct
      961 cgagccgggc gaagccgcag accagggtct cgccgtagcg ctccttgaac tcgtgaaact
     1021 cgctgtcgtc gaccaggcgc gcgatgacct cgtggacgtc gtaggccacc cgcgggtcgg
     1081 ccgggatgac gccgtacagc tcatcgggat tgaccgatgg cggcctcgct gcggccggct
     1141 cccacggcgg ctccggcttg cgccaggcca ggttgcggac gatcgatcga ccaatcgcca
     1201 gggcgtgctc atcggtcagc gcgtagtgat cggccacgcc gctggtcacg gtatggactt
     1261 cggccccgcc caggtcctcg gcgctcacct cctcgccggt cgcggccttc accagcggcg
     1321 ggccgccgat gaagatggcc cccgtgccgc ggacgatcac cgtctcgtcg ctcatggccg
     1381 gcacgtatgc cccgcccgcg gtggagtgac ccatcacgat cgcgatctgg ggaatcccac
     1441 gcgacgacag ccgggcctgg ttgaagaaga tccgaccgaa gtgctcgcgg tcggggaaca
     1501 cgtcgtcctg aagggggagg aacgcgccgc cactgtcgac caggtacagg cacggcagcc
     1561 ggttctcgag ggcgatctcc tgggcgcgga gatgcttctt cacggtcatc gggtagtacg
     1621 tgccgccctt caccgtcgcg tcattggcga cgatcacgca ctcgaccccc tccacccggc
     1681 cgatgccggt gaccatcccc gcgcccgggg cgtcgtcgtc atacaggccg ttggcggcca
     1741 gggtcgagag ctcgaggaag gcagtgcccg ggtcgatcag ctggtcaacc cgctcgcgga
     1801 cgaggagctt cccgcgctcg cgatggcggg cgatggccct ctcgtccccg cccgcgccgg
     1861 cgctcgtgac ccgcgccgtt cgttcgtgca ggtcggcaac gagctcgcgc atcgccccgg
     1921 cattggcggc gaaggtctca gaggcaggat cgaccgcgga gcggagaacg gccatgtcgg
     1981 ccgattatgg ggcgggccga tgcggcccgg atcggtcagg cgaggctgac cagcgggacc
     2041 agggtctcga cgtacttcgt attgacgatc acgaccgaga cgacgtgctc gaaccggggc
     2101 tcgccgtgat ggaggagatg ggcctcggtg agtgggatga atccgtgcat ctgatacggc
     2161 tcggggaacg gggccccggg ggtgatgtgg aggttgcccg tgaccgtgaa cgtgccggtg
     2221 tcggccgcga cccgctggat gcgccgatgg agacggagct ggcggttcgt ccgccactcc
     2281 ggcggcatga ccaccacgac gtcctcggtg ggcaccgtgc gccactcccc ggacttgtcg
     2341 agcatgggtc gcacggtcat gtcgtcgccc cccgcgctcg gctgccagag gcgcagctcg
     2401 tcgctggcca ccagcaggtc ggtcacgcgc tggccgttgg acgcgatcca gcccacgacc
     2461 cgggtgtcgg gcgtgtacag ctcgatcggc gcgaggtcgg tgccgttggc gagagcggct
     2521 gagccggaag ttgccgaccc accacttccg cgccgccagt tccagaaggt cacgccccga
     2581 agtttgccgg gtggccgact ccgcgaacag cacacgagag tactgcccga atgggaaccg
     2641 aacggcggtg cgcggcggca acagccggac tcgcccgcgc cgatcgccgg tccgctatac
     2701 aggtaccaat ggctgaaggc gctccccccg agacgaccgt caagcggttc ctcgaggtcg
     2761 atatgcgggt cggccggatc gtgtccgcgg agccgttccc cgcggcccgg aacccgtcat
     2821 accggctgcg catcgacttc ggccccgaca tcggggagcg ggagtctgcc gcgcagctga
     2881 cggtcacgta ccccgacccc gcgaccctca tcggtcggca ggtggtggcg gtcgtgaact
     2941 tcccgccccg tcggatcgcc ggattcgcga gccaggtcct cgtcctgggg gccatgggcg
     3001 agggtggtca cgtgttcctc ctcgaacccg acccggcgtc ccccgaaggg ctccggatcg
     3061 ggtgatcggc tgatcctggc cgatgggcga tactccaccg gccgtgacca tcgccaacca
     3121 gccgttgctc ggagacatcc ccgacggcat gcccgacccg catgttgcgc cgccgcaggt
     3181 ggcccgcgcc ggggacccgt tcgcgtcgct gcggatcgtg gtcggcctct ctcgcttgtc
     3241 gcgcaacctg acccatcagc tccgggacgt ggtcaccgcg ttgaacgcgg cgtacatcga
     3301 ctggtcgttc accgaggacg tcgtcctgtc cgagctcgtg cagctccagt ccaactggag
     3361 catctcgttc cacggcgagg accggatcgt cctgggcatg agcgaacggg ggcggaccat
     3421 cctcatcgtc gactcgacca agatgacgcc gttcttcctg tcccaggcac aggcccaggc
     3481 cgaggcctgc gacgcagccc tccgcgagtt cgcgatcggc gacgggaact actgagcgcg
     3541 caggactgac cgatggaacc gaaacccgtc acctgtacgg cgaccctcac cggcgagaag
     3601 ggggagtact acttcatcgt gccggtcgat ttccaggccg tcttcggccg ccatcggccg
     3661 ccggtgcggg tcacgatccg gggccacacc tttcgcacca ccccggctcg ctacggcagc
     3721 aagtacttcg tggtcgtgaa ccgcgcgaac cgcgaggcga tgggcgggct tgaccacggc
     3781 gaccgggtcg ggattcggat cgagctcgac accgagcccc gcgtcgtcaa gccgccaccc
     3841 gagctgacgg cgacgctcgc cgccaacgcg gaggccgccg cggtctacac gcagctctcg
     3901 ttcagccacc agaaggcgta tgccgactgg gtagccgagg ccaagcgccc cgagacccgc
     3961 gtccgccgag cgggcgaatc ggcgaggcgc ctgctcgcgg gtcggaagga gccgagcggc
     4021 tagggcgggt ttaagatcgg acgatgaatg agctcctcag gaaccgactc gtcgcgctgg
     4081 cgatcctggc tctcccgctg ctcgtcgcgg cctgcaacag cggcggcggc ggaggcgccc
     4141 cgggctactg agcgggtctc tgcctcagaa gcggtcggcc tgggcggcct ggccgacggc
     4201 ggcctctggc gcttccatcg ccacggcgac cttgtccgtt cgacgcggct ttggccggaa
     4261 cggagacgac tcggggacga tcggtccatc ggtctcggcc agctcgccgc cgcgataggc
     4321 ctcccatacg gccatgaccg tgtcgcggtg tggccgaatc cggcagtgct cgtcgcggtt
     4381 gcccatctcg tcgcagtagc ccagcggcac gcacttgggg gccaggaacg acccgaagaa
     4441 gggtgagacc cggaagattt cgtggcggat ctgcttgaac agctggcgga tctcccactg
     4501 ggccatggtg cacagccgca gcccgctcat gtggatgagc tggcggaggt tcacggtcat
     4561 gatcaggttg gtctgcatcg cgttcgggaa gatgaaccgc gcatcctcgg ccgggacctc
     4621 ggcctggagg agctggccgt agaggtccag gttctcgtca acggctcgct ggaagcgacc
     4681 cgccaggtcg tcgggcaggt cgcccttcgt gatcgagtcg gggatcgtgt agttgtcccg
     4741 gcccttgaac gagacatatc gctgcgactg ctggtcgaag gccgttcctg cccgatggcg
     4801 gacgagctgg tgggaaagcg tccgagtcac gccactgatc gcaaacgtga agttcacgtg
     4861 ctcgatggtc gatccgtggc cggattccat gaccttgcgg acgagggcct gctgcttctc
     4921 gtcggcgacc gatcgggaga cggccttgtc ccagatgacg tccggcagca gctcggagta
     4981 gcacgtccgg caggcggtgt agatgatcgg gaggtagtac tcctccacga tctccgcggt
     5041 cgggaagatc agcttcgcgg acaggttcgg cgaggtcggc ggcatggtct tggcagcagt
     5101 cccggcagat tcggagcagt ggtggtaagg cgccgagtct agcaccgggc aggggccaaa
     5161 aggaggccgt tatccacaac ctgtagtggt gtggataacc tcaggccgat atcttgtgtc
     5221 gttcggtgtc cgccgcaagc cgagaaaagg aggccctagg cctcctcctc gtcgaccgtt
     5281 cggccctcgt cggtcggggt cggcgaatca tggatgcgat ccctctcggc agaggacatc
     5341 aactcctacg tcggacgcta cggaaacacc cactagggct agaggccctc acagacgacc
     5401 caaggctccg cttgcccatc cgggcaagcg gagtccaccg gtcccagtcg ccagacgagc
     5461 aatgtcacta cgacggcaat gagagcagcc gacagaagca accaccgcca cgaggccacc
     5521 atcgcgttca ccgccggagc ctagcaccca tggcacgcaa gatcacagaa tcgatcgtca
     5581 gggggtaggt gtccgcggca agcgggaaac ccgacgggcc ctagcccgaa cgctcccgcg
     5641 cggcatcctc gttccttgtc cgcactacta agcctcgccg ttgtcctcga gtggctcttc
     5701 gtcggtcggg gtttccctgc gtcgttcgtc gaggcgggcc aggccgtgga gtcggtcgta
     5761 ggcaatgacg ttgccaccgc cggcccgggc cgaggcgacc gcttcatcgg cgcgggcgag
     5821 aagcgtgtcg gcgctgagct cctgccgcga gcgcatggtc gtgaccccga tcgagatcgt
     5881 gactgtcacc tcgccgcgcc ccgtcgagat cggccgctgg gtggcccgat cgcggatcgc
     5941 ctcggcgaac acggtcgcac cctgttcatc ggtatgggga aggacggcca ggaagctgtc
     6001 gcccgccatc cgaccgaggg cgtcagcctc gcggacgcgg acccggattc ggagtgcgag
     6061 ctcgcggagg acgtcatccc cgatcgcggt gccatgccgg gcgttgatct ctcccatccc
     6121 gtccacgtcc atgagcacga ccgacaccga gtgcggatat cggcgggccg aagccacctc
     6181 gtcgcggagc cggtccgtga tcgcgcttcg gctgagcacg ccggtgagcg cgtccgcctc
     6241 ggcggctcgc tcgagttcgg tgcgctggag ggcgatggtg tgccgggcat cggtcagctc
     6301 gatcagagcg gcccgcagct cgcggccgat ccgctcgcgc tcggggtcgt cggccggcgg
     6361 caggggagtc gcggcggcag cgacctcgat gaccggtgcg gattcggaat cgcgggcgag
     6421 gcccgacact acttcgagga cctgggcgcg gatcgcgaac ccggccacca ggagtggcgg
     6481 cgcgagcagg agcggggtca gcgcgatcac ggcctcgacc gtgcccgtcc gggaccaggt
     6541 cagcagcacg gcggcaagcg caacggtcac cggcgcgagc gcgatgaggc gggctccacg
     6601 gcggaaccag accgcggcac tggccgcggc caccacggcc gcacccatga acaacatggg
     6661 ggtgatgctg tcgagggctg acccgggtgc gagcagcgcg gcggcgagtg cggtctccgc
     6721 caggacgagg gccaggatca cgcccatcca gcggctacgc tcgtcagcga tcgtgcggta
     6781 cccggcccac gctgccacgg caaagccgac cgcgacgacg accagggtca gcgcgagtcc
     6841 gctgttcggg agtgacgctg atccttccgg cccgctgagc gcgagcgccg cggtgccggc
     6901 ggtgagtgcg gttgcgctca ggccggccga cccagtcgcc aggcgtccgg acgtgaccag
     6961 cccgcccatc aacggaacga ccgagatggc cgagcacagc gcgaccaggc cgagggccgc
     7021 agggccaagc caaccggtct cgccagccgg gtccagggtc agtggcggga acaggcgcag
     7081 cgcggccacc gcggccgcgg ggagcgccag ggccagcccg gtgcccaccg cccgcgcaat
     7141 cgctgcggtc aactcggatc ggagcatgtt tcgccggtgc gtgttgtcac cgcgagtgtc
     7201 ctgatcgctg ggggctacgg gcaatagtac gaacggcccg gtcgcgagtc gtttcggcct
     7261 catctgcgct tccgctggac ctcgccgccg cgagcatact cggcgcatgc agaccgagcc
     7321 ggtcatgacc cggagcgcct tcctggctgc gaccgatgcc ctgatcgtag agggcgaccg
     7381 attggccgtg acgcccgact gggacgggtt tcgcgcatgg ctgctcgact cggaccaact
     7441 gttggaacag gtatggggac ggatggaccg atatcacctg gcctggctca acgtcggtcg
     7501 tgactccgcg ccgccgggat ccccattgga cgaagcgggt acggcccgat tcgtgaccga
     7561 ggtcgcgagc gccaaggtcg ccgtcctgcg tacgatgcgc accgcggtcg agcgccaggg
     7621 atggcgccgg ctgtcggacg accccgtgga gagcccgtga cctacgaccg aaccggcggc
     7681 atggccgtca tcctcgccca cccggccgac gaggccttca cctgtgccgg ctcgctggcc
     7741 ctggcccacg acgcgggcga gaccacgagg ctcctgatcg tgacccgggg cgaggccggc
     7801 aacccggagc ggaccccgga cgccgacctg gccgcggttc gcgagtccga gatggtgcgg
     7861 gccgcggagg ccatcggact gaacgaggtg accctgctgg agggctatcc cgatggtgcg
     7921 gtggccgccg agcccaccga gcggctcatc caggagctct tcgcctggct cggcaaccgc
     7981 cgcccggacg tgctcgtcac gttcggctcg cacggtgtga ccggtgatcc ggaccacgtg
     8041 accgtgggca gcgcggctcg ctgggcggtc gagcgtctcg cgcgccaggg tgtcgcgccc
     8101 aactcgctct acgtcatcgc gcc
//
LOCUS       DASYGU010000162         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_849562, whole genome shotgun sequence.
ACCESSION   DASYGU010000162 DASYGU010000000
VERSION     DASYGU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849562"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VGO32_04460"
     CDS             <1..610
                     /locus_tag="VGO32_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200038.1"
                     /translation="MYPGIIAMGVLTTSLFAGVSVVWDREFGFLRELLVAPLGRTGIV
                     LGKAAGAAVVALLQVALMLLIAPIVGVELSLQMIVLLVPIVFILSIALSGLGLLVASY
                     MRSQQGFQLLIQLLIFPLIFLAGVFFPINQAPEWLQVVSKFNPLTYGVDAIRQVMIGS
                     ADPRVGVTVLGHTMTLGEEVLLIGVFAVALLTAAAWAFNRQE"
     gene            620..1105
                     /locus_tag="VGO32_04465"
     CDS             620..1105
                     /locus_tag="VGO32_04465"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEV7200039.1"
                     /translation="MSRLPPADRDPFPRERFGALVRRLPRYGRLAWTLSRHPKVTSRR
                     RMALVGAAVYLVSPIDLVPGFIPVAGQLDDAAAILIAIGVALRSMQPADRAAVLAEAG
                     VDAADIDEDLRAIRDSYAWMARKGIRLGARATRLAARAVTGVVSGIRGRLRGRGKPGA
                     D"
     gene            complement(1108..1770)
                     /locus_tag="VGO32_04470"
     CDS             complement(1108..1770)
                     /locus_tag="VGO32_04470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7200040.1"
                     /translation="MRALIFDLDGTLIDTVYAHVFAWQRALAEEGMPIEGWRIHRKIG
                     MSGGLFTRALARELGRPIADADVEEIQRRHGDLYRDLLPERRPLPGAVELLGHLRFIG
                     IPYGIATSGRRPEIDDSLAALGIGDEVVVVERGDVMRAKPEPDLFLACQERMGVEIRD
                     VTVVGDAVWDLLAARRAGMLSVGLLTGGYGEDELTQAGAFRVYRDTAELDASLDELGI
                     VP"
BASE COUNT          247 a          647 c          597 g          297 t
ORIGIN      
        1 catgtacccc ggaatcatcg cgatgggggt gctgaccaca tccctgttcg ccggcgtgtc
       61 ggtcgtgtgg gaccgcgagt tcgggttcct ccgcgagctg ctggtcgcgc ccctcggccg
      121 aaccgggatc gtgctcggca aggcggcagg tgcggcggtg gtggccctcc tccaggtggc
      181 gctcatgctc ctcatcgcgc cgatcgtggg cgtggagctc agcctccaga tgatcgtgct
      241 cctggtcccg atcgtgttca tcctgtccat cgcgctgtcc gggctgggcc tgctggtcgc
      301 cagctacatg cgatcgcagc agggtttcca gctgctcatt cagctcctga tcttcccgct
      361 catcttcctg gccggggtct tcttccccat caaccaggca ccggagtggc tccaggtcgt
      421 gagcaagttc aacccgctga cctacggagt ggacgccatc cgccaggtga tgatcgggtc
      481 ggcggatccc cgcgtggggg tgaccgtgct cggccacacg atgaccctgg gcgaggaagt
      541 cctcctcatc ggcgtcttcg cggtcgccct gctgaccgcc gccgcgtggg cgttcaaccg
      601 ccaggagtag gctctggggg tgagcaggct ccctcccgct gaccgcgatc cgtttccgcg
      661 tgaacggttt ggcgcgctcg tccggcgcct cccccgatac ggccgcctgg cgtggacgct
      721 cagccgccac cccaaggtca cgagcaggcg acggatggcc ctggtcggcg cagccgtcta
      781 cctcgtctcg ccgatcgacc tcgtccccgg cttcatcccg gttgccggcc agctggacga
      841 cgccgcggcg atcctgatcg cgatcggggt cgcgctccgc agcatgcagc cggcggaccg
      901 tgccgccgtc ctcgccgagg ctggggtcga tgccgcggac atcgacgaag acctccgcgc
      961 cattcgcgac tcgtacgcgt ggatggcgcg caaggggatt cggctgggcg ctcgcgcaac
     1021 ccgactcgcc gcgagagccg tgaccggcgt ggtctccggc atccgggggc gcttgcgggg
     1081 tcgaggaaaa ccgggggcgg actgagccta gggaacgatc cccagctcgt cgagcgaggc
     1141 gtccagctca gcggtgtcgc ggtacacacg aaatgcgccg gcctgggtca gctcgtcctc
     1201 cccgtagccg ccggtcaaca gccccacgga cagcattccc gcgcgccgcg cggcaagcag
     1261 gtcccagacc gcgtcgccga ccaccgtcac gtcgcggatc tcgaccccca tccgctcctg
     1321 gcaggcgagg aacaggtcgg gctccggttt cgcgcgcatg acgtccccgc gctcgacgac
     1381 gaccacctcg tcgccgatcc cgagcgcggc cagcgagtca tcgatttcgg gccgccggcc
     1441 ggaggtcgcg atcccgtacg ggatgccgat gaaccgcagg tggccgagca gctccaccgc
     1501 tcccggcagt ggtcgccgtt ccgggagcag gtctcggtac aggtcgccgt ggcggcgctg
     1561 gatctcctcc acatcggcat ctgcgatggg cctgcccagc tcccgcgcca gggcacgcgt
     1621 gaagaggccg ccactcatcc cgatcttccg gtgaatgcgc cagccctcga tcggcatccc
     1681 ctcctcggcc agcgctcgct gccaggcgaa gacgtgcgca tagaccgtgt cgatgagcgt
     1741 gccgtcaagg tcaaagatca gcgcccgcat cccggtcaag ggtagcgg
//
LOCUS       DASYGU010000163         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1724343, whole genome shotgun sequence.
ACCESSION   DASYGU010000163 DASYGU010000000
VERSION     DASYGU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724343"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGO32_04475"
     CDS             complement(<1..211)
                     /locus_tag="VGO32_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEV7200041.1"
                     /translation="MRILLVGVGTVGESIARLAAPHAWCESMVLADSDTARAEALQAS
                     LADPGRFPVEHLDARDRGAVAELARR"
     gene            complement(305..769)
                     /locus_tag="VGO32_04480"
     CDS             complement(305..769)
                     /locus_tag="VGO32_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200042.1"
                     /translation="MNVPFKRPVVAVLGASLLLLAACSPSASGDAGGGGSTVDITLQE
                     FAVIASETSAPAGEITFSVTNDGPDDVHEFVIVQTDLEPGDLPTDEHGTVDESGEGME
                     VIDEIEDLPVGETQEVTVELEAGSYVLLCNIFDATEDEAHYAEGMRSAFTVE"
     gene            complement(791..1693)
                     /locus_tag="VGO32_04485"
     CDS             complement(791..1693)
                     /locus_tag="VGO32_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV7200043.1"
                     /translation="MEETVAATRDQWLAADNAPARWGARLGPVLFVLVIATSFTFAGV
                     ASNLTIDYLAWDSRAYYDALRSADPYAGAAVGEIGSFLYPPPFLQVLGPAGQLPWPLF
                     LFGWTALLATAMVVMLRRVPQRYRFLWPLLIFLGGADIWAGNINLLLAVGAVLALTYP
                     MAWAGLALTKVTAGVGMVWHAFRGQWPELGLAVSVTVVGAALSFIAAPDLWADWLGVV
                     FGESPISLYATSLPIPLFVRLPLAIGLLWWAARTDRVWLVPCACLIALPVIWFNGLSM
                     LLGAAALLPSGTGPIRRDGEDSAH"
     gene            1776..>2068
                     /locus_tag="VGO32_04490"
     CDS             1776..>2068
                     /locus_tag="VGO32_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV7200044.1"
                     /translation="MRERLNWIDWLKVLVVVGAFLFHAAQPFVYTTWLINDTDKSLPL
                     SLASGFGYLFGMPLMFFLAGAATWLAVERRGIGGHTGLRLRRLLIPLVLGLII"
BASE COUNT          354 a          699 c          712 g          303 t
ORIGIN      
        1 ggcgccgggc aagctcggcc acggcgccgc gatcacgggc atcgaggtgc tcgaccggga
       61 agcgtccggg atcggcgagc gatgcctgga gggcctccgc gcgggcggtg tcggagtcgg
      121 cgaggaccat ggactcgcac catgcgtggg gcgcggccag ccgggcgatg gactcgccga
      181 ccgtcccgac tcccaccagc agaatgcgca tgcggtcctc ccacgcgtgg atccctcacg
      241 cccgccgact ggcgaacgcg ccgctgtcgg cggcgcgtcc ggagtctatc ggtctggctt
      301 ccgcctattc gaccgtgaat gcggaccgca tgccctcggc gtagtgggcc tcgtcctcag
      361 tcgcatcgaa gatgttgcac agcaggacgt agctgccggc ctccagttcg acggtgacct
      421 cttgggtttc gccgaccggg aggtcctcga tctcgtcaat gacctccatg ccttcgccgc
      481 tctcgtccac ggtgccgtgc tcgtcggtcg gcaggtcgcc gggctcgaga tcggtctgga
      541 cgatgacgaa ctcatgcacg tcatccgggc cgtcgttggt caccgagaac gtgatctctc
      601 cggccggtgc tgacgtctcg gacgcgatca ccgcgaactc ctggagggtg atgtcgacgg
      661 tcgatccgcc cccacctgcg tctccgctcg cgctcgggct gcatgcggcc agcagcagaa
      721 gactggctcc aagcacagcg acgacgggtc gcttgaacgg tacgttcatg ggtctctcct
      781 caatgagacc ctagtgtgcc gagtcctcgc cgtctcgtcg aatgggcccg gtcccggaag
      841 gtagaagggc cgcggcgccg agcagcatcg agaggccgtt gaaccagatg accggcaggg
      901 cgatcaggca tgcgcagggc accagccaca cgcgatcggt gcgcgccgcc caccacagca
      961 ggccgatggc cagcggcagg cgaacaaaga gcgggatggg tagcgatgtc gcatacaggg
     1021 agatggggct ctcaccgaac acgacgccca gccagtcggc ccacaggtcc ggggcggcga
     1081 tgaacgacag ggccgcgccg accacggtca cggataccgc caggccgagc tcgggccact
     1141 ggccgcgaaa cgcgtgccac accattccca cgccggcggt gaccttggtc agggccaatc
     1201 cggcccaggc catgggatag gtgagcgcga gcaccgcgcc caccgccagc aggaggttga
     1261 tgttgccggc ccagatgtcc gccccgccaa ggaagatgag gagcggccag aggaatcggt
     1321 accgctgcgg gacacgacga agcatgacga ccatcgccgt ggccaacagt gcggtccagc
     1381 cgaacaggaa gagcggccac ggcagctggc cggccgggcc gaggacctgc aggaacggcg
     1441 gcggatagag gaacgagccg atctcgccaa cggccgcccc ggcataggga tccgcgctgc
     1501 gcagcgcgtc gtagtaggca cggctgtccc acgccaggta atcgatggtc aggttggacg
     1561 cgacgccggc aaacgtgaac gaggtggcga tcaccagcac gaagaggacc gggccgaggc
     1621 gcgcacccca gcgagccgga gcattatcag ccgccagcca ctggtcgcgg gtcgccgcga
     1681 cggtctcctc catcggaccc gatcctacgg gaccgatgca accgggggga agagccgaac
     1741 ggcccgtggt tggaggcctc tggcagacta cggccgtgcg tgagcggctg aactggatcg
     1801 actggctgaa agtgctggtg gtggtcggcg cctttctgtt ccatgccgcg cagccgttcg
     1861 tctacacgac ctggctcatc aacgacaccg acaagagcct gcccctgagc ctcgcctcgg
     1921 gcttcggcta cctgttcggg atgccgctca tgttcttcct ggccggtgcg gcgacctggc
     1981 tagccgtcga gcggcgagga atcggcggcc acaccgggct ccgccttcgg cggctgctca
     2041 tcccgctcgt cctgggcctg atcatctt
//
LOCUS       DASYGU010000164         5595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_180969, whole genome shotgun sequence.
ACCESSION   DASYGU010000164 DASYGU010000000
VERSION     DASYGU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180969"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2542
                     /locus_tag="VGO32_04495"
     CDS             <1..2542
                     /locus_tag="VGO32_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7200045.1"
                     /translation="LPITSPGHGGESLLVWTTTPWTLSSNVAAAVHPDLVYDLVESAG
                     GERWWVSRGSRARVASDAPVVGEAQGKDLLGLTYRGPFDELPVQDGVAHMVIAWDEVT
                     DDEGTGIVHIAPGCGEEDFALGKRDGLAVLDPIDEFGVFKEGYGWQTGRFAGATPEAA
                     DDLAPAIASDLEAKGLLVARETYRHHYPVCWRCNTQLLFRLVDEWFIAMDPLREPISD
                     VTRAVHWVPDGIGLEERELDWLRNMRDWMISKKRYYGLALPFWVCEECAGWEVIGSKE
                     ELHERAVAGWEAFEGHSPHRPWVDGVEIVCQACGGRSRRIRDVGNPWLDAGIVAYSTL
                     KWDTDPEYWAQWFPVDFLTESFPGQFRNWFYALLTMSTVLTDRAPTRTLLGHALVRDV
                     HGEEMHKSRGNAIWFDDAAEEIGADVMRWLYAASNPAVNVNFGYEPGAEVVRRFFLPL
                     WNTYSFLVTYARLDGWVPGDTSASGTSLLDRWILSRLDGLVDEVRAALDAYDAARGAR
                     VIEAFVDDLSNWYVRRNRRRFWKGELDDDKQAAYATLYEVLTSVIRVMAPFVPHLTDA
                     VWHNLVVSVDPAAPDSVHLADFPVGRGRADADTDRGVALARRVVGLGRTARAGSEIRT
                     RQPLRRVRVRLPAGMTAFAADPRTAAELEDQVRDELNVKEVELITEDSELVERALYPL
                     LPVIGPRHGADVGRIMAAVRSGEWSLTDDGGALAGGVALAADEFTLTSRARPGHEIAD
                     EADTLVALDTTIDEELAAEGMAREVAHRLQSMRKAAGLEISDRIEIAIGVIAADAERL
                     APHRDWLAAETLATGLALGPDADLADSMAREELTLDGSTLRLALRRAG"
     gene            2562..3068
                     /gene="lspA"
                     /locus_tag="VGO32_04500"
     CDS             2562..3068
                     /gene="lspA"
                     /locus_tag="VGO32_04500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV7200046.1"
                     /translation="MSRLPLRNVWAESGVLLATGAVVYLADRLTKAWVVANVDIGEQI
                     PVIGDVLQIWHTENEGAAFGLLPGGGIVFLVVGFVTLIAIGWVHFTGRVRGAAAAVLL
                     GMILGGTLGNLTDRLIDGSVTDWVSVGIGSIRWPTFNVADSSVVVGILGLILLLSIHD
                     RRTATNTA"
     gene            3065..4054
                     /locus_tag="VGO32_04505"
     CDS             3065..4054
                     /locus_tag="VGO32_04505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV7200047.1"
                     /translation="MIVSGERILEGTAPAGRVDVAVATVAGISRAHAQRLLGDGRALV
                     DGKPRRASDRLIGGETLQVTLSAPADPTLVPEAIPIQVVYEDEDLLVVDKAAGMVVHP
                     SAGHPTGTLVHALLGRSLSRGESLGSVAGVGRPGIVHRLDRDSSGLLVVAKTDAAQAS
                     LMAQFGSRTVSKEYLALVRGRAPAAQGRIEAPVGRDPKDRQRMAVVADGRDAVTEYDA
                     VGAGRGYTLLRLHPHTGRTHQLRAHLAYLDLPIAGDLRYGGGAGPGGLSRQFLHASGL
                     AFQRPSDEARVAVWSELPEDLRAALEETGIDATGLPDTGYTIDGQPIDPEGAG"
     gene            4051..4662
                     /gene="gmk"
                     /locus_tag="VGO32_04510"
     CDS             4051..4662
                     /gene="gmk"
                     /locus_tag="VGO32_04510"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV7200048.1"
                     /translation="MSKRMAYPNPMLVVVSGPSGVGKSTIVAEMARLHPQAVPIVTAT
                     TRDRRPGEIDGVHYHFMTAEAFERLRDDGGLLEWAEVHGHWYGTPLDQIRGILRAGRD
                     AVLTIDPQGARTVRAAVPDALLIFVMPPSMEALDARLSQRGSEDAEAQALRRRNAVDE
                     IAAAADYDEIVVNETGHGGEAAEQIWEIIQAEARRDPPRRVRL"
     gene            4673..>5595
                     /locus_tag="VGO32_04515"
     CDS             4673..>5595
                     /locus_tag="VGO32_04515"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200049.1"
                     /translation="MSSPEEPASDGRFVRVAVDAQVDRPERTFTYRWPADLPDEFSGE
                     AGSLVLVPYGGRLALGYLLGDAREPLPDALPIETVVSRPLLTQDLIDLAEAIAIRYRA
                     PMGTTLAAMLPPGMESRLERRWEILEPLGDVPVGSVLTESQLSRLAPRAGPRWIESRR
                     RAGALRAAWRLRPPTAGPRHERTIRLLSRPDGKVTGAVQRVILDALGPDEIALADLAT
                     ELGRSPSSLLAPARRLEAAGSVELGWHAVRRDPLAHRTPGRTAADSMAPEQAAALAEI
                     GELAPAGELLLEGVAASGKSDVILAAVTATVA"
BASE COUNT          775 a         1955 c         2037 g          828 t
ORIGIN      
        1 gctgccgatc acgagcccgg gccacggggg cgagtcgctc ctggtctgga cgaccacccc
       61 gtggacgctc tccagcaatg tcgcggccgc cgtccacccg gatctcgtct atgacctggt
      121 cgagagcgcc ggcggcgagc gctggtgggt gagccgcggg tcacgggcgc gcgtggcctc
      181 cgatgcaccg gtggtgggcg aggcgcaggg gaaggacctg ctgggactga cctaccgcgg
      241 gccgttcgac gagctgccgg tccaggacgg ggtggcccac atggtcattg cctgggacga
      301 ggtgaccgat gacgagggga ccgggatcgt ccacatcgcc cccggctgcg gtgaagagga
      361 ctttgccctc ggcaagcggg atgggctggc ggtcctggac ccgatcgacg agttcggggt
      421 gttcaaggag ggctacggtt ggcagaccgg gcggttcgcg ggagcgaccc ccgaagcggc
      481 tgacgatctc gcccccgcaa tagcctccga cttggaggcc aagggcctgc tcgtcgcgcg
      541 cgagacctac cgccaccact acccggtctg ctggcgctgc aacacgcagc tcctgttccg
      601 gctggtggac gaatggttca tcgccatgga ccccctccgc gaaccgatca gcgacgtgac
      661 ccgcgcggtg cactgggtgc cggatgggat cggcctggag gagcgtgagc tcgactggct
      721 ccggaacatg cgtgactgga tgatcagcaa gaagcggtac tacgggctcg ccctcccgtt
      781 ctgggtgtgc gaggagtgcg cgggctggga ggtcatcggc tccaaggagg agctccacga
      841 gcgggcggtg gccggctggg aggcgttcga gggtcacagc ccgcatcggc cgtgggtgga
      901 tggagtcgag atcgtgtgcc aggcatgcgg tggccggtcg cgccggatcc gcgacgtggg
      961 caacccgtgg ctcgacgcgg ggatcgtggc ctactcgacc ctgaagtggg acaccgatcc
     1021 cgagtactgg gcgcagtggt tcccggtcga cttcctgacc gaatccttcc ccggccagtt
     1081 ccgcaactgg ttctacgccc tgctcacgat gtccaccgtc ctgaccgatc gcgcgccgac
     1141 ccggaccctg ctcgggcacg ccctggttcg ggacgtgcat ggcgaggaga tgcacaagag
     1201 ccggggcaac gcgatctggt tcgacgacgc ggccgaggag atcggtgccg acgtcatgcg
     1261 ctggctgtat gccgcgtcca atccagcggt gaacgtgaac ttcggctacg aaccgggggc
     1321 cgaggtggtt cgccgcttct tcctgcccct gtggaacacg tacagcttcc tggtcaccta
     1381 cgcccggctg gacgggtggg tccccggcga tacctcggcg tccgggacct ccctgctgga
     1441 ccgatggatc ctctcgcggc tggacggcct ggtggacgag gtgcgtgccg cgctggacgc
     1501 gtacgacgct gcgcgcgggg cccgggtgat cgaggccttc gtcgacgatc tatcgaactg
     1561 gtacgtccga cgcaaccggc gccgcttctg gaagggcgag ctggacgacg acaagcaggc
     1621 tgcctacgcc actctgtacg aggtcctgac cagcgtcatc cgggtcatgg cccccttcgt
     1681 gccccacctg accgacgccg tgtggcacaa cctcgtggtc tcggttgatc ccgccgcccc
     1741 cgattcggtc cacctggccg acttccccgt cggccggggc cgtgccgacg ccgacaccga
     1801 ccggggcgtg gcgctcgccc ggcgggtggt cgggctcggt cggaccgccc gagcggggag
     1861 cgagattcgc acccggcagc cgctgcgccg ggtgcgggtc cgcctgccgg ccggaatgac
     1921 ggccttcgcc gccgatccgc ggacggccgc cgagctggag gaccaggtcc gtgacgaatt
     1981 gaacgtgaag gaggtcgagc tgatcacgga ggactccgag ctggttgagc gggccctgta
     2041 cccgctcctg ccggtgatcg gcccgcgaca cggggccgat gtgggccgga tcatggccgc
     2101 ggtccgatca ggcgaatggt cactgaccga tgacggaggc gcgctcgcgg gaggggtggc
     2161 cctggccgcg gacgagttca cgctcacgtc gcgcgcacgg ccgggccacg agatcgccga
     2221 cgaggccgac acgctggtcg cgctggacac gacgatcgat gaggagctgg cggccgaggg
     2281 catggcccgc gaggtcgccc atcggctcca gtccatgcgc aaggcggccg gcctcgagat
     2341 cagcgaccgg atcgagatcg cgatcggcgt gatcgccgcg gatgcggagc ggctggcccc
     2401 gcaccgggat tggctggcgg ccgagacgct ggccaccggg ctggccctcg gtccggatgc
     2461 cgacctggcc gattcgatgg cacgcgagga gctcacgctc gacggcagca ccctccgcct
     2521 ggccctgcgc cgggcgggat gatcgcgtac gctcggggga catgagccgt ctgccgctcc
     2581 gcaacgtctg ggccgagagc ggagtcctgc tcgcgaccgg cgcggtggtc tacctcgccg
     2641 atcggctgac caaggcgtgg gtcgtggcca acgtcgacat cggcgagcag atcccggtca
     2701 tcggtgacgt cctccagatc tggcacaccg agaacgaggg cgccgcgttc gggctgctgc
     2761 cgggtggcgg catcgtcttc ctcgtcgtcg gattcgtgac cctgatcgcc attggctggg
     2821 tccatttcac cggccgggtc cgcggcgcgg ccgcggccgt cctgctgggc atgatcctcg
     2881 ggggcacgct tggcaacctc accgaccgcc tcatcgatgg cagcgtcacc gactgggtct
     2941 cggtcgggat cggcagcatc cgctggccga cgttcaacgt ggccgactcg tcggtcgtgg
     3001 tcggcatcct gggcctgatc cttctcctgt ccatccacga ccgccggacg gcgacgaaca
     3061 ccgcgtgatc gtgagcgggg agcggatcct ggaaggcacc gcccccgccg gacgggttga
     3121 cgtggccgtc gcgacggtgg ccggcatctc gcgagcccac gctcagcgcc tgctgggaga
     3181 cggccgcgcc ctggtcgacg gaaagccccg ccgggcgagc gatcgcctca ttggcggcga
     3241 gacgctgcag gtcacgctgt ccgcgcccgc ggacccgacg ctcgtgccgg aggcgatccc
     3301 gatccaggtc gtgtacgagg acgaggatct gctggtcgtg gacaaggcgg cgggcatggt
     3361 cgtccatcca tcggcggggc acccgaccgg gacccttgtg cacgctctgc tgggccgatc
     3421 gctttcccgt ggggagtcgc tcgggtcggt cgccggcgtt ggtcggccgg gaatcgtcca
     3481 ccgcctcgac cgcgactcct ccggcctgct ggtggtggcc aagaccgatg cggcccaggc
     3541 gtcactgatg gcccagttcg ggtcgcgcac ggtgagcaag gagtacctcg cgctcgtccg
     3601 cggccgcgcg ccggccgcgc agggccggat cgaggcgccc gttggccgcg acccgaaaga
     3661 ccggcaacga atggcggtcg tggccgacgg ccgggacgcc gtgacggagt acgacgcggt
     3721 cggcgctggt cgcgggtata ccctgctccg cctgcacccg cataccggcc gcacccacca
     3781 gctccgcgcc catctcgcat acctggacct gcccatcgcc ggcgatctcc gctacggcgg
     3841 cggcgccggg cccggcggcc tgtcgcgcca gttccttcat gcgtcggggc tcgcattcca
     3901 gcgtccgtcg gacgaggccc gggtagcggt ctggagcgaa ctcccggagg atctgcgcgc
     3961 ggcgctcgag gagaccggga tcgatgccac cgggcttccg gacaccggct atacgatcga
     4021 cggccagccg atcgatccgg agggcgcggg atgagcaagc ggatggccta ccccaacccg
     4081 atgctggtcg tggtcagcgg accgtcgggc gtgggcaaga gcacgatcgt ggccgagatg
     4141 gctcgccttc atccccaggc ggtcccgatc gtgaccgcca caacccggga ccggcgcccg
     4201 ggcgagatcg acggcgtcca ctaccacttc atgaccgccg aggcgttcga gcggctccgc
     4261 gatgacggcg ggctgctcga gtgggccgaa gtccacggcc actggtatgg cactccgctg
     4321 gaccagatcc gcggcatcct ccgcgcgggt cgggacgcgg tcctgactat cgaccctcag
     4381 ggggcgcgga cggtgcgcgc cgccgtgccc gatgcgctgc tcatcttcgt catgccgccc
     4441 tcgatggagg cgctggatgc ccggctctcg cagcgcggct cggaggacgc cgaggcgcag
     4501 gccctgcgac ggcgcaacgc ggtggacgag attgcggccg cggcggacta cgacgagatc
     4561 gtcgtcaacg agaccggtca cggcggcgaa gcggcagaac agatctggga gatcatccag
     4621 gccgaggctc gccgagatcc tcctcgtcgc gtccgcctct gagccgcgtt tgatgtcctc
     4681 cccggaggag cccgccagcg acgggcgatt cgtccgggtc gcggtcgatg cccaggtcga
     4741 ccggccggaa cggaccttca cctaccgatg gccggccgac ctgccggacg agttctcggg
     4801 tgaggccggg tccctcgtgc tcgtgccgta cggcggccgc ctcgcactcg ggtacctcct
     4861 cggtgacgcg cgcgagccgc ttccggacgc cctcccgatc gagacggtcg tctcccggcc
     4921 gctcctcacg caggacctca tcgacctggc cgaggcgatc gcgatccgat atcgcgcccc
     4981 gatgggaacg accctggccg cgatgctgcc gccgggcatg gagtcgcgtc tcgagcgccg
     5041 gtgggagatc ctcgagccgc tgggagacgt ccccgtcggg tcggtcctga ctgaatcgca
     5101 gctgagccgc ctcgcccccc gagccggtcc gcgctggatc gagagccggc gccgggccgg
     5161 cgcgctgcgt gcggcgtggc ggcttcgacc tcccaccgcc ggcccgcgcc acgagcggac
     5221 gatccgcctg ctctcccgac ccgacggcaa ggtcaccggc gcggtccagc gcgtgatcct
     5281 ggacgcgctc ggccccgatg agattgccct ggcggacctc gccacggagc tcggacgttc
     5341 cccgagcagc cttctggcgc ccgcgcggcg gctcgaggcg gcagggtcag tcgagctggg
     5401 atggcacgcc gtgcgccgcg atccgctggc ccaccggact ccgggccgca cggcagccga
     5461 ctccatggcc cccgaacagg ccgcggcgct ggcggagatc ggtgagctgg cgcccgcggg
     5521 cgagctgctg ctcgagggcg tcgccgcctc gggcaagtcg gacgtcatcc tggccgcggt
     5581 caccgcgacg gtggc
//
LOCUS       DASYGU010000165         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1621744, whole genome shotgun sequence.
ACCESSION   DASYGU010000165 DASYGU010000000
VERSION     DASYGU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621744"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGO32_04520"
     CDS             <1..892
                     /locus_tag="VGO32_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7200050.1"
                     /translation="EIFGFLGPNGAGKTTTINMLCTLLSPTAGSAKVNGFDVRHERSE
                     VRRSIGLVFQQTTLDEYLSAEQNLRFHAHAYGIPAEVREQRMTELLNIVDLADRRKGS
                     VRTYSGGMKRRLEIARGLLHHPKVLFLDEPTLGLDPQTRRLIWDYLLALRDKEGLTIF
                     LTTHYMDEAEHSDRIAVIDHGLIAGLDTPNGLKRSVGGDLVTIAAPDNQAAAIEITDR
                     YGAEAAVRDGTVSFSVSNGANFLPDFVRTFSQPLQSVTVREPTLDDVFLHLTGREIRD
                     SELDSQEQLVAGMARRFGRG"
     gene            903..1694
                     /locus_tag="VGO32_04525"
     CDS             903..1694
                     /locus_tag="VGO32_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200051.1"
                     /translation="MTEVWRGTWVVGYRELLRFVNERSRMIGSFAQPLIFLVIFGAGF
                     GNLIGSVAPGVDFIQFMYPGIIAMGVLTTSLFAGVSVVWDREFGFLRELLVAPIGRTG
                     IVLGKAAGAAVVALLQVALMLLIAPIVGVDLDVGIIVLLVPIVFILSIALSGLGLLVA
                     SYMRSQQGFQLLIQLLIFPLIFLAGVFFPINQAPEWLQVISKFNPLTYGVDAIRQVFL
                     GSDAGPRVGVTVLGHVMTLGEEVLIIGAFAVVLITAAAWSFNRQE"
     gene            1717..2208
                     /locus_tag="VGO32_04530"
     CDS             1717..2208
                     /locus_tag="VGO32_04530"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEV7200052.1"
                     /translation="MNHEPLREPDPFPRDRFTAMLRRMPRYGRLAWNLSRHPKVTGKR
                     RAALIAAAVYLVSPIDLVPGFIPVAGQLDDAAAILIAIGVALRSMQPAERATVLAEAG
                     VEAADIDEDLRTIRDVYAWMARKGVRLGARATRLAARAVTGVVSGIRGRLRGRGKPGA
                     DGA"
     gene            complement(2205..>2432)
                     /locus_tag="VGO32_04535"
     CDS             complement(2205..>2432)
                     /locus_tag="VGO32_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEV7200053.1"
                     /translation="LFLACQERMEVEIRDVTVVGDAVWDLLAARRAGMLSVGLLTGGY
                     GEDELTQAGAFRVYRDPAELDASLDELGIVP"
BASE COUNT          358 a          883 c          777 g          414 t
ORIGIN      
        1 cgagatcttc ggcttcctcg gtccgaacgg ggcgggtaag acgacgacca tcaacatgct
       61 ctgcaccctc ctcagcccga ccgccgggtc cgccaaggtc aacggctttg acgtgcgcca
      121 cgagcgaagc gaggtccgcc gctccatcgg tctcgtcttc cagcagacca ccctggacga
      181 gtacctgagc gccgagcaga acctccgctt ccacgcccat gcgtacggca tcccggccga
      241 ggtgcgcgag cagcggatga ccgagctcct gaacatcgtc gacctcgccg accgccggaa
      301 gggctcggtc cggacctact cgggcggcat gaagcggcgg ctcgagattg cccgtggcct
      361 gcttcaccat cccaaggtcc tgttcctcga cgagccgacc ctcggactgg acccccagac
      421 ccggcgtctc atctgggact acctgctcgc gctccgcgac aaggaggggc tgaccatctt
      481 cctgaccacc cactacatgg acgaggccga acattcggat cggatcgcgg tcatcgacca
      541 cggcctgatc gcggggctcg acacgcccaa cggcctcaag cgctcggtcg ggggcgacct
      601 cgtgaccatc gcggcacccg acaaccaggc cgcagcgatc gagatcactg accgatacgg
      661 cgcggaggcc gcggtccgcg acgggacggt cagcttcagc gtgtcgaacg gggccaattt
      721 cctgcccgac ttcgtgcgga ccttcagcca gccgctccag tcggtcaccg tgcgcgagcc
      781 gactctcgac gacgtgttcc tgcacctgac cggccgcgag atccgcgact cggagcttga
      841 ctcccaggag cagctggtgg cgggcatggc ccgccgcttc ggccgcggat gaggatgaat
      901 cgatgaccga agtctggcgc ggaacctggg tcgtcggcta tcgcgagctg ctccggttcg
      961 tgaacgagcg gtcgcggatg atcgggtcct ttgcccagcc gctgatcttc cttgtcattt
     1021 tcggggccgg cttcggcaac ctcatcggct cggtcgcccc cggcgtcgac ttcatccagt
     1081 tcatgtaccc cggcatcatc gccatggggg tcctgaccac gtccctgttc gcgggcgtgt
     1141 cggtcgtgtg ggaccgcgag ttcgggttcc tccgcgagct gctggtcgct cccatcggcc
     1201 ggaccgggat cgtgctcggc aaggcagcag gggccgccgt ggtggcgctc ctccaggtgg
     1261 cgctcatgct cctcatcgcg cccattgtgg gcgtggacct ggacgtcggg atcatcgtcc
     1321 tcctggtccc gatcgtgttc atcctgtcca tcgcgctgtc cgggctgggc ctcctggtcg
     1381 cgagctacat gcgctcccag cagggcttcc agctgctcat ccagctcctg atcttcccgc
     1441 tcatcttcct ggcgggggtc ttcttcccga tcaaccaggc acccgagtgg ctccaggtca
     1501 tcagcaagtt caacccactc acctatggcg tggacgccat ccgccaggtc ttcctgggct
     1561 ccgatgccgg gccgcgggtc ggcgtcaccg tgctgggcca tgtcatgacc ctgggtgaag
     1621 aggtcctgat catcggcgcc ttcgccgtcg tcctcatcac cgccgccgcc tggtccttca
     1681 accgccagga gtagccgctc ggagtaggct ctgggcgtga accacgaacc gcttcgcgaa
     1741 cccgatccgt ttccgcgcga tcgattcacg gccatgctcc ggcgcatgcc gcgctacggc
     1801 cggctggcct ggaacctcag ccgccatccg aaggtcaccg gcaaacggcg cgcggccctg
     1861 atcgccgctg cggtgtacct ggtttccccc atcgacctcg tgccggggtt catcccggtc
     1921 gccggccagc tggacgatgc ggccgcgatc ctgatcgcca tcggcgtcgc cctgcgcagc
     1981 atgcagccgg ccgagcgggc caccgtcctt gccgaggccg gggtcgaggc cgcggacatc
     2041 gatgaggatc tccgcaccat ccgcgacgtg tacgcgtgga tggcgcgcaa gggggttcgc
     2101 ctgggcgcgc gtgcaacacg cctcgccgcg agagccgtga ccggcgtggt ctccggcatc
     2161 cgggggcgct tgcggggtcg aggaaaaccg ggggcggacg gagcctaggg aacgatcccc
     2221 agctcgtcga gcgaggcgtc cagctcggcg gggtcgcggt acacccggaa cgctccggcc
     2281 tgggtcagct catcctcccc gtagccgccc gtcagcagcc ccaccgacag cattcccgcc
     2341 cgccgggcgg cgagcaggtc ccagaccgcg tcgccgacca ccgtcacgtc gcggatctcg
     2401 acctccatcc tttcctggca ggcgaggaac ag
//
LOCUS       DASYGU010000166         7827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1210482, whole genome shotgun sequence.
ACCESSION   DASYGU010000166 DASYGU010000000
VERSION     DASYGU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210482"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGO32_04540"
     CDS             <1..744
                     /locus_tag="VGO32_04540"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200054.1"
                     /translation="EGLRTVVLLGALPLLVYLATYIGVADGSLVAMSWREGSWFYDIA
                     HHQLAMARFHAGLEGHHPYESPSWSWFLLKRPVAFWFTPDPGDYPFRLYEQILSMGSP
                     LAWWPALAVFGWLGVSWLRGNRSLAVSVILVGALSAYLPWLVLSFARSQIFLWYVLPT
                     LPFLYAAVGVAVIRWRGWARGLLVVGLAGALAGFWFFWPIAIASPLSPDDWQLRMWFT
                     DCERSGAPTLQRPDDTINSGPPPDGWCWI"
     gene            854..1981
                     /locus_tag="VGO32_04545"
     CDS             854..1981
                     /locus_tag="VGO32_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEV7200055.1"
                     /translation="MRTAILNRSVTFVVLVITGMAMVIGSAALRVGAADHLDAPSLGS
                     LSVGSLKGDRDINDVYVFPVSGNRTVLAMTTNPAVNVPSIDPFGTYATNVQYRINVDR
                     DGDFVQDISYVTTFGRPGTAGVQNFTIKRYRDGAAIHASGPGQWVASGFTDNARGNPN
                     VAQGVRAFAGERSDPFFFDLIGFRGTLGLDPSTTQRLCSVAPDPNPDGTGTDFFAPLN
                     TNAIVLQVPDAALGKNIAVWADTRQFINGKWVIVDQMGRPAINTVFNSGADKESFNVT
                     PPAYQDNAGKPYRTNVSNVLKALGGYSQSAADGLAAALIPDAITYDTSSMGTNILNGR
                     ALSDDVIDAELKIVLQNQAASDCVPWDGDSLPNFPYLDNPQ"
     gene            2048..3430
                     /locus_tag="VGO32_04550"
     CDS             2048..3430
                     /locus_tag="VGO32_04550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7200056.1"
                     /translation="MTTLVRLFRVRLVPIVGAAAILVAITYVASALTTPPPTPEGAPP
                     GAMAGELTPAAPPAGPIAGLLDRYDGAIRQWSESIDASDANYLAATTLGMTYAGRARL
                     TGDLSDYQRALEAADAALDVSPDYLPARELRASVLFSLHEFAAARDEAQAVWEADPGA
                     LQALAVVGDASLELGDLDRARSAFATLGEREASPPVWSRLAHLAFVEGDVVRAIELVE
                     SAVASSSRESTPEEAAFYAFQLGDLYRASGRTTEAGAAYEQALAILPEHVPATAGLAR
                     VREAQGRRAEAISLLEAAVARLPQPELVAALGDLHALAGDGAAAEEQYALVDRIGEVS
                     AATGSVYDRQLVLFDADHDRHVAAAVDLAEAAVRVRHDIYGYDALAWALFHAGRLDEA
                     AAAADEALALGTRDPRLAYHAGMIAAARNDVDTARRLLTQAIAGAAYLPPLQVPIAEQ
                     ALAALGEVDR"
     gene            3427..4938
                     /locus_tag="VGO32_04555"
     CDS             3427..4938
                     /locus_tag="VGO32_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200057.1"
                     /translation="MIRRVVAALSVLAAAFLLSTSPTSAHPLGNATVNRATAVTLGPD
                     WIDIASIVDMAEIPAYAAILDIDSNGDEVVQPGEGAAWADATCDAAAAALRVTVDGQP
                     VAIAAADAPLLTFPPGVGGLETLRLVCPLSGEVPPGGAVREVSVTNLADDDRRGWREV
                     TIAAGSGVAIESADVPSASPSALLTAYPQGLLQAPIAVSAGSATYRAAARAGSATPGG
                     GGPATDAASVHLGWGSQTDALVDLLDGGAAPWSWLLAVAVAAGLGAAHAVSPGHGKAL
                     IAAYVIGSQGSMRRAAGLGLTVAASHTVGVFFLGAIVLSATELLVPDRVVAWLSVASG
                     VLVVGIGASVARRAWRSRRPELNPPHSHPHGRSHPHGHSHPHDRSGAHGQGQDPGGVS
                     DRPPATREFLTLGLVGGMVPSTSALLVLLVAVATGRLVEGMILIVAFGAGMAAVLGGL
                     AAATALARRGLAESATGHHGSRISRLAGAVPLASAVVIIMVGVVTTVGALGNL"
     gene            5022..5576
                     /locus_tag="VGO32_04560"
     CDS             5022..5576
                     /locus_tag="VGO32_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7200058.1"
                     /translation="MDNAGLAGRIRGGDREALGELYDRHAGQALAVAFRIVGDRSTAE
                     DIVHDAFVVVWQRIDQFDSDRGTLRAWLLTIVRNRAIDGIRGTRPTMTVGDADQQSLL
                     RTAPNPTWEQASARMSAIEIRGMLETLPAEQREAIELAYFGGRTYREIARITGVPEGT
                     ASGRLRLGLAKLRSGLQDTMEQDR"
     gene            5573..6337
                     /locus_tag="VGO32_04565"
     CDS             5573..6337
                     /locus_tag="VGO32_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV7200059.1"
                     /translation="MTRPSREPMGCSEVNGLAGALALGAVDQVELAEVMDHLASCVEP
                     HEELRALAGAPLALASSLEPMTPNPAVRARLMRTVAQQTNQHAGSPAAPVDRGRRGRG
                     AWGSVNVWRGLAVAAVLAVLAFGVWNVSLRIQLATRDQALSAVAQAIAGGYPAYPISG
                     PAGTGYVIDTEGSGSTFLVAGLESLPRGELYEMWLIDADGTPLAVGTIDQSNADLVVA
                     TLEQDLAGFDVFAVTVESEPVEAPTTDPVMAGPITN"
     gene            complement(6420..7013)
                     /locus_tag="VGO32_04570"
     CDS             complement(6420..7013)
                     /locus_tag="VGO32_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200060.1"
                     /translation="MREVHGRSFALVAGALLALAACGPVASPSPSVSVPSPSAPASAF
                     ESPLYGYSVELSGVWRPDPATEAWTGTETFGSDSANSDKFHSDRGTTIWAVAAPTTKT
                     LDELVAERIADDAAENDCPTEAESDEDITVSGQPARLTTKHCPADAADGGALIVMATV
                     LDQGDVYFFYFIYPVYLASDPNAIDAFTSFLDGVELP"
     gene            complement(7081..7656)
                     /locus_tag="VGO32_04575"
     CDS             complement(7081..7656)
                     /locus_tag="VGO32_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200061.1"
                     /translation="MRSSPLQTAAIVLIVAAKFVIPLLIIRFPFAAGWANFVLDGIDG
                     DLLIPLGLPNDIYQPVDKITDWVTYVAMVVVGYRSQWPIRRLMLGLFLFRSIGQVAFL
                     LTGEELFLALFPNFLEPLFLVTATILAWQRVVRHNPDWREASFAFLRRHRWPIGILIV
                     VYKLQDEYFTHVANVDRSDFLQQLLERLSGG"
     gene            complement(7696..7771)
                     /locus_tag="VGO32_04580"
     tRNA            complement(7696..7771)
                     /locus_tag="VGO32_04580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7736..7738),aa:Arg,seq:gcg)
BASE COUNT         1178 a         2671 c         2699 g         1279 t
ORIGIN      
        1 gagggcctcc ggaccgtggt gctcctgggc gcgctgccgc tgctggtcta cctcgccacc
       61 tatatcggtg tcgctgatgg ttcgctggtc gccatgtcat ggcgcgaagg cagctggttc
      121 tacgacatcg cccaccacca gctggcgatg gcgcgtttcc acgccgggct cgaggggcac
      181 cacccgtacg aatcgccatc gtggtcgtgg ttcctcctga aacgtcccgt cgcgttctgg
      241 tttaccccag atccgggcga ctaccccttc cgcttgtatg agcagatcct gtcgatgggc
      301 agtccgctgg cgtggtggcc ggcactcgcg gtgttcggtt ggctgggagt gagctggctt
      361 cgcgggaatc ggtcgctggc cgtctcggtc atcctggtcg gggcgctctc ggcctacctg
      421 ccgtggctgg ttctcagctt tgcgcggagc cagatcttcc tgtggtacgt cttgccgacc
      481 ttgccgttcc tctacgcggc ggtgggggtg gccgtcatcc gctggcgggg ctgggctcgt
      541 ggcctgctgg tcgtcggcct ggccggagcg ctggcgggtt tctggttctt ctggccgatc
      601 gccatcgcgt cgccgctcag ccccgatgac tggcagctcc gcatgtggtt caccgactgc
      661 gagcgatccg gggcaccgac cctccagcgc cccgacgaca cgatcaactc cggtccccca
      721 cccgacgggt ggtgctggat ctaggcggtt aggtgcactc cagtgatgca tttccttcgc
      781 ccggccgtag tccctccaga acgtcgcgca tctccgcgcc ggcggctact tattttcgga
      841 gggactcgat cccatgcgaa cggcaatcct caaccgctcg gtcaccttcg tcgtgctggt
      901 catcaccggc atggcgatgg tcatcgggtc ggccgcgctg cgtgtcggcg ccgctgacca
      961 cctcgacgcg ccgtccctcg gctcgctgag cgtgggctcc ctgaagggag accgcgacat
     1021 caacgacgtg tacgtcttcc cggtctcggg caaccggacc gtgctggcca tgacgaccaa
     1081 cccggccgtc aacgtcccgt ccatcgatcc atttggcacg tatgcgacga acgtccagta
     1141 ccgcatcaac gtcgaccgcg acggcgactt cgtccaggac atctcctatg tcacgacgtt
     1201 cggccgaccg gggacggctg gcgtgcagaa cttcacgatc aagcgctacc gcgacggagc
     1261 cgcgatccac gcctccggac cgggtcagtg ggtcgcgtcc ggcttcacgg acaacgcccg
     1321 aggcaaccca aacgtcgccc agggcgtccg tgccttcgcg ggcgagcgct cggatccgtt
     1381 cttcttcgac ctgatcgggt tccgaggcac gctgggactc gacccctcga ccacccagcg
     1441 cctgtgcagc gtggctccgg atccgaatcc agatgggacc ggaacggact tcttcgcccc
     1501 gctcaacacg aacgcgatcg tcctgcaggt gcccgacgcg gcactcggca agaacatcgc
     1561 ggtctgggca gacacgcgac agttcatcaa cggcaagtgg gtcatcgtcg accagatggg
     1621 tcggccggcg atcaacaccg tgttcaactc gggcgctgac aaggagtcct tcaacgtgac
     1681 tccgccggcg taccaggaca acgctggcaa gccgtaccgg accaatgtct cgaacgtgct
     1741 caaggcattg ggcgggtaca gccagtcggc cgccgacggc ctggccgcgg cgctcattcc
     1801 cgacgccatc acctacgaca cgagctcgat ggggaccaac atcctcaacg gccgtgccct
     1861 gagtgatgac gtcatcgacg ccgagctgaa gatcgtgctt cagaaccagg cggcgagtga
     1921 ctgcgtgcca tgggacgggg attcgctccc gaacttcccg tacctggaca acccccagta
     1981 gcaaaccgat gtcgggcggg cggctccggc cgcccgcccg acgcggcctg acttggagtc
     2041 tgcatccatg accacgctcg tccgcctctt ccgcgttcgc ctcgtgccga tcgtcggcgc
     2101 ggccgcgatc ctcgtggcca tcacctacgt cgcgagcgcg ttgaccaccc cgccgccgac
     2161 tccggagggc gcaccgcccg gtgcgatggc aggcgagctt actcctgcgg ccccgcccgc
     2221 cggcccgatc gccggcctcc tcgaccgata cgacggcgca atccgccagt ggtccgagag
     2281 catcgacgcc agcgatgcaa actacctggc agccacgacc cttggcatga cctacgcggg
     2341 ccgagcgcgc ctgaccggcg acctttccga ctaccaacgg gcgctggagg cggctgacgc
     2401 cgcattggac gtgagccccg actatcttcc cgctcgagaa ctccgcgctt ccgtcctctt
     2461 ctcgctccat gaattcgcgg ccgcgcggga cgaggcgcaa gccgtgtggg aggccgatcc
     2521 gggagcgctg caggcgcttg cggtcgttgg cgacgcgagc ctcgagctcg gcgacctcga
     2581 ccgcgcccgg tcggccttcg caaccctggg cgaacgggag gcgtcgcccc cggtgtggag
     2641 ccgcctcgcc cacctcgcct tcgtcgaggg cgatgtcgtg cgggccatcg agctcgtcga
     2701 atcggctgtg gcgagcagct cccgggagtc cactcctgaa gaagccgcct tctacgcgtt
     2761 ccagcttggc gatctgtacc gcgcatccgg ccgcacgact gaggcaggcg ctgcatatga
     2821 gcaggccctg gccatcctgc ccgagcatgt tcccgccacg gccggcctgg cgagggtccg
     2881 ggaggcgcag ggccggcgcg cagaagccat ctcgctgctg gaggcggcgg tggccagact
     2941 cccgcagccg gagctcgtgg cggcgctcgg cgatctccat gccctcgccg gcgacggtgc
     3001 ggcagccgag gagcaatacg ccctggtcga tcggatcggc gaggtgtcag cggcaaccgg
     3061 gtcggtctat gaccgacagc tggtcctgtt cgacgcggat catgatcgac atgtcgccgc
     3121 cgcagttgac ctcgcggagg ccgcggtcag ggtgcggcac gacatctatg gctacgacgc
     3181 gctggcctgg gccctgttcc atgccggtcg gctcgatgag gccgcggcag cagccgacga
     3241 ggccctcgcg ctggggaccc gcgacccgcg actcgcgtac cacgccggga tgatcgcggc
     3301 cgcgcggaac gacgtggaca cggcgaggcg gctccttacc caggccattg ccggcgccgc
     3361 ctacctgcca ccgctgcagg tgccgatcgc ggagcaggcc ctcgccgcgc tcggggaggt
     3421 ggaccgatga tccggcgggt cgtggcggcc ctgtccgtgt tggcggccgc atttctgctc
     3481 agcacatccc cgacctcggc gcatccgctc ggtaacgcga cggtgaaccg cgcgacggcg
     3541 gtgaccctcg gccccgattg gatcgacatc gcgtcgatcg tggacatggc agagatcccc
     3601 gcctacgcgg ccatcctgga catcgattcg aacggcgatg aggtcgtcca gcctggcgaa
     3661 ggggccgcgt gggcggacgc gacctgcgac gcggccgcgg cggcgctgcg ggtgaccgta
     3721 gatggccagc cggtggcgat cgcggccgcc gacgcgccgt tgctcacctt cccgcccggc
     3781 gttggcgggc tcgaaaccct tcgcctggtc tgtccgctgt cgggtgaggt cccacccggc
     3841 ggcgcggtcc gggaggtgtc cgtgacgaac ctggccgatg acgaccggcg cggatggcgg
     3901 gaggtcacca tcgcagccgg gtcgggtgtc gccatcgagt cagccgacgt gccgtcggcc
     3961 agtccctcgg cgctgctgac cgcctatccg cagggcctgc tccaggcacc gatcgccgtt
     4021 tccgctggat cggcgaccta ccgcgcagcc gctcgcgcgg gttccgcgac ccccgggggc
     4081 ggcggaccgg ccaccgatgc ggcctccgtc cacctcggct ggggcagcca gactgacgcg
     4141 ttggtggatc ttctcgacgg aggcgcggcc ccttggtcct ggctgctggc ggtcgcggtc
     4201 gcagcggggc tgggagcggc gcacgccgtc tcgccgggcc acggcaaagc gctcatcgcc
     4261 gcgtacgtaa tcggctcaca ggggtcgatg cggcgtgccg ccggcctggg tttgaccgtc
     4321 gcggcctcgc acacggtcgg cgtgtttttc ctgggggcga tcgtgctctc ggctaccgag
     4381 ctgctggttc cggatcgcgt cgtcgcgtgg ctgtcggtgg cctccggcgt gctcgtggtg
     4441 ggcatcgggg ccagcgtggc gcgacgcgcg tggcgctcgc gacgccctga actcaacccg
     4501 ccccattccc atccgcatgg ccgctcccat ccgcatggcc actcccatcc gcatgaccgt
     4561 tccggcgcgc atggccaagg gcaagacccg ggcggggtga gcgatcgacc tccagccacc
     4621 cgcgagttcc tgaccctcgg cctggtcggg gggatggtgc ccagcacgtc agcgctcctc
     4681 gtgctcctcg tggcggtggc gaccggacgg ttggtggaag ggatgattct catcgtggcc
     4741 ttcggggccg ggatggccgc cgtgctcggc ggcctggccg ccgccacggc tctcgcccgg
     4801 agaggtctcg ccgagtctgc caccggccat catggttcgc gcatctcccg gctggccggc
     4861 gccgtgccgc ttgcatcggc agtcgtgatc atcatggtcg gggtggtcac cacggtcggc
     4921 gccctgggca acctgtgagg gcttccatcg tggccaccgg gtctgtccga tggctcgcga
     4981 tcgcggtcgg accagcatcc gagactgggt attctgaggt cgtggataac gcgggcctgg
     5041 cgggccgcat tcgcggtgga gaccgcgaag cgctggggga gctctatgac cgccatgcgg
     5101 gtcaggccct cgcggtcgcg ttccggatcg tgggcgatcg gtcgaccgcc gaggacatcg
     5161 tccacgacgc gttcgtcgtg gtctggcagc gcatcgatca gttcgattcg gatcgcggca
     5221 ccctgcgcgc ctggctgctg accatcgtgc gcaatcgggc catcgatggg attcgcggca
     5281 cgcggcccac gatgacagtg ggtgacgccg accagcagtc gctgctgcgc acagcgccga
     5341 atccaacatg ggagcaggcc tcggcccgga tgtcggcgat cgagatccgg ggcatgctcg
     5401 agacgctccc ggccgaacag cgcgaagcga tcgaactcgc atacttcggg ggccgaacct
     5461 accgcgagat cgcgcgaatc actggcgtgc cggaagggac cgccagcggc aggctgcgac
     5521 tgggcctggc caagctgagg agtgggctgc aggacacgat ggagcaggac cgatgacgag
     5581 gccgtcgcgc gagcctatgg gctgcagcga ggtgaacggg ctggccggcg cgcttgcgct
     5641 gggggccgtg gaccaggtag agctcgccga ggtcatggat cacctcgcga gctgcgtcga
     5701 gcctcacgaa gaactgcgtg cgttggcggg cgcgccactc gcactcgcgt cgagcctcga
     5761 gcccatgacc ccgaatcccg cggtgcgggc gcgactcatg cgaaccgtgg cccagcagac
     5821 caaccaacac gcgggttcgc cggcggcgcc ggtcgatcgc ggtcggcggg ggcggggggc
     5881 gtggggctcg gtgaacgtgt ggcgggggct ggccgtcgcc gcggtgctgg ccgtcctggc
     5941 gtttggcgtg tggaacgtgt ccctccgcat acaactagcg acgcgggatc aagccctgtc
     6001 cgccgtcgcg caggcgattg ccggcggcta ccctgcctac cccatcagcg gtccggcggg
     6061 gaccggatac gtcatcgata ccgagggatc cggttccacc ttcctggtcg ctggcctcga
     6121 gtcgctgccg cgcggcgagc tgtacgagat gtggctcatc gacgccgatg ggacaccgct
     6181 ggcggtgggc accatcgacc agtcaaacgc cgacctcgtc gtcgccacgc tcgagcagga
     6241 tctggccgga tttgacgtgt tcgcggtgac ggtcgagtcg gaaccggtcg aggcccccac
     6301 caccgatccc gtgatggcgg gcccgatcac gaactgacgg actcgacagg agcgcgaaca
     6361 ttggagctcc ctgacgacac gatcaactcc ggtcccccac ccgacgggtg gtgctggatc
     6421 tagggaagct ccacgccgtc gaggaacgag gtgaacgcgt cgatcgcgtt tgggtcggag
     6481 gcgaggtaca ccgggtagat gaagtagaag aagtagacat caccttgatc gagcaccgtg
     6541 gccatcacga tcagggcacc gccatcggcc gcatccgcgg ggcagtgttt cgtggtcaga
     6601 cgagccggtt ggccgctgac tgtaatgtcc tcgtcagact ccgcctccgt cgggcagtcg
     6661 ttttccgccg cgtcgtcagc aatccgctcg gcgacgagct cgtccaacgt cttcgtggta
     6721 ggcgccgcga ccgcccagat ggtggtgccc ctgtcagagt ggaacttgtc cgagttcgcc
     6781 gagtcgctgc cgaacgtctc ggttcccgtc caggcttcgg tggccggatc cggccgccat
     6841 acgcctgaca gttcgaccga gtagccgtag aggggggact cgaaggcgga tgcgggtgcc
     6901 gacggcgagg gcacgctgac cgacggtgag ggcgacgcga cgggaccaca ggcggcgagc
     6961 gccaggagcg cgccggcgac cagtgcgaag gatcgtccgt gaacctctct caccctcggc
     7021 ccacgatgcg ctgcgaccct tggagtgtca accgatcgac tggctcggtg cgcgtcgccg
     7081 ctatccgccg gagagccgct cgaggagctg ttggaggaag tcgctgcggt cgacgttggc
     7141 gacgtgggtg aagtactcgt cctggagctt gtagaccacg atcaggatcc cgatgggcca
     7201 ccgatggcgg cgcaggaacg cgaacgatgc ctcccgccag tcggggttgt gacggacgac
     7261 ccgctgccag gccaggatgg tggctgtgac caggaatagc ggctcgagga agttcgggaa
     7321 gagcgcaagg aacagctcct ccccggtcag caggaaggcg acctggccga tagagcggaa
     7381 caggaacagc ccgagcatca gccggcgaat cggccactgg ctgcgatacc ccacgacgac
     7441 catcgccacg taggtcaccc agtcggtgat cttgtcgacc ggctggtaga tgtcgttggg
     7501 caggccgagc gggatgagga ggtcgccgtc gatcccgtcg aggacgaagt tggcccagcc
     7561 cgccgcaaac gggaaccgga tgatgagcag cgggatgacg aatttcgccg caacgatcag
     7621 cacgatcgcc gcggtctgga gcggactgct acgcatctcg cgccacgccg gcagtgtgac
     7681 gcctgccgac aggagtggcg tgcccgagag gactcgaacc tccgaccttt aggtccgcaa
     7741 cctaacgctc tatccgctga gctacgggca cccgagacga tctggcggag agggagggat
     7801 tcgaaccctc ggaagaggaa atcccct
//
LOCUS       DASYGU010000167         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_283541, whole genome shotgun sequence.
ACCESSION   DASYGU010000167 DASYGU010000000
VERSION     DASYGU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283541"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VGO32_04585"
     CDS             <1..500
                     /locus_tag="VGO32_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEV7200062.1"
                     /translation="EAAAESPFHGRIADSVVPQIDRLFDDGWTGEERKVIAELRKILD
                     LPDLDVAATTVRVPVHTGHSVALHVRTVRPTTLEAIESALRAAAGVEYRAADGSDRHP
                     MPLDVEGRDPVLVGRLRAIPGRERGFALFVASDNLRKGAALNAIQVAAAFDPDRFGCL
                     LPEPR"
     gene            497..1321
                     /locus_tag="VGO32_04590"
     CDS             497..1321
                     /locus_tag="VGO32_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV7200063.1"
                     /translation="MTRRLPLRIAIAAGVVLVAAVAIGFGLGRLLSAPGESPTPSASP
                     SDAASCQVTVTPTTIHVDPSNGSSDDVLFFAGAGFPPNAAIQIDFGATDAVEELETTT
                     GGTFDAAIPASVGTTYPADVEAGPLTWTVHGLPEPGSSPGGSPAAEPSCTAEVTVTVA
                     LTAAASPTPPTDLSADGYAEILADGVRVRVEPSIDATVVGALFTGDVVRILAPAQVVD
                     GLAWYRIESVVTQSGDQVSGYVAAGADGQNFLRPTGEPPPPTPSPSPSASPSASPT"
     gene            complement(1333..>1737)
                     /locus_tag="VGO32_04595"
     CDS             complement(1333..>1737)
                     /locus_tag="VGO32_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200064.1"
                     /translation="DNEGYIEQLKYANRRTLEAIDQIIANTPPGDPEPIIILQADEGP
                     FPPRFREQGADFQWLEATDDEILWKFNVLTAYRLPGVDAEAEGFSDRISPVNAFRIVF
                     NAYFDAGLELLPDVTYLSPDHDHMWSFVEYPR"
BASE COUNT          241 a          643 c          578 g          275 t
ORIGIN      
        1 gcgaggcagc cgccgagtcg ccgttccacg gacgaatcgc cgactcggtc gtgccccaga
       61 tcgaccgcct gttcgacgat ggctggacgg gtgaggaacg gaaggtcatc gccgagctgc
      121 gaaagatcct ggacctgccc gacctcgacg tggccgcgac cacggtacgg gtcccggtcc
      181 acaccggcca ctcggtcgcg cttcacgtcc ggacggtgcg cccgacaacg ctcgaggcga
      241 tcgaatccgc actccgcgcc gcggcgggag ttgagtaccg cgccgccgat ggatcggacc
      301 gccatccgat gccactcgac gtggagggcc gcgacccggt gctcgtcggc cggctgcgcg
      361 cgatccccgg ccgcgagcgc ggattcgccc tgttcgtggc cagcgacaac ctgcgcaagg
      421 gcgccgcgtt gaacgccatc caggtggcgg ccgccttcga cccggaccgg tttggctgcc
      481 tcctgcctga acctcgatga cccggcgcct ccccctgcgc atcgcgattg ccgccggggt
      541 cgtcctcgtc gccgcggtcg ccatcggctt tggtctgggc cggctcctct ccgccccggg
      601 cgaaagcccg acgcccagcg cctcaccgtc ggacgccgcg tcgtgccagg tcacggtcac
      661 cccgaccacc attcacgttg atccctccaa tggcagctcg gatgacgtcc tgttcttcgc
      721 cggcgccggc ttcccgccga acgccgccat ccagatcgac ttcggagcca ccgatgcggt
      781 tgaagagctc gagaccacca ccggcggtac gttcgacgcc gcgattcccg cctcggtggg
      841 caccacctat cccgctgacg tggaggcggg cccgctgacc tggaccgtcc acggcctgcc
      901 cgaacccgga tcgtcgcccg gtggctcccc ggccgccgag ccgtcgtgca cggccgaggt
      961 cacggtgacc gttgccctca cggctgccgc gagccccacc ccgcccaccg acctgtcggc
     1021 cgacggctac gccgagatcc tggccgacgg cgttcgggta cgggtggagc cgtccatcga
     1081 cgcgaccgtg gtcggcgccc tgttcaccgg tgacgtggtc cggatcctgg cacccgccca
     1141 ggtcgtcgat ggattggcct ggtaccggat cgagtcggtc gtcacccaga gcggcgacca
     1201 agtgagtggc tacgtggcgg cgggtgccga cggtcagaat ttcctccgcc cgaccggcga
     1261 gccgccaccg cccacgccgt ccccgtcacc gtccgcctcg ccttcggcgt cgccgaccta
     1321 gtccagactg gcctagcgcg ggtactccac gaagctccac atgtggtcgt ggtcggggga
     1381 gaggtaggtg acatcgggca gcagctccag ccccgcgtcg aagtaggcgt tgaacacgat
     1441 ccggaacgcg ttcaccgggc tgatccggtc ggagaagccc tcggcctcgg catcgacgcc
     1501 gggcagccgg tatgcggtca ggacgttgaa cttccagagg atctcgtcgt cggtggcctc
     1561 cagccactgg aagtccgcgc cctgctcccg gaagcgcggc gggaagggac cctcgtcggc
     1621 ctggaggatg atgatcggct ccggatcgcc tggcggggtg ttggcgatga tctggtcgat
     1681 ggcctccagc gtccgccggt tggcgtactt cagctgctcg atgtagccct cgttgtc
//
LOCUS       DASYGU010000168         8239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_645127, whole genome shotgun sequence.
ACCESSION   DASYGU010000168 DASYGU010000000
VERSION     DASYGU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645127"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="speB"
                     /locus_tag="VGO32_04600"
     CDS             <1..915
                     /gene="speB"
                     /locus_tag="VGO32_04600"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEV7200065.1"
                     /translation="ALAARRPDVVILGAPLDDGVSHRPGARFGPRAIRTASYHSGDIH
                     SLQVGVDPFDWLDVVDGGDAPVVPGRLERGHAVIRARVLEIARTGAIPIVLGGDHSIT
                     YPSASAVAEAVAPRRLGIVHFDAHADTGDNTWGNLLSHGTPMRRLIQSGAVAGRNFVQ
                     VGLRGYWPPAETLAWMREQEMRWHLMTEIEARGAEAVVHDAIAEALDGPDVVYLSVDI
                     DVIDPGMAPGTGTPEPGGMLTRELLRAIRQVVGAVDLVGMDVVEVAPAYDVSDVTAAA
                     AHRCVLEAISALAGRRRDARESARPTVR"
     gene            complement(922..2157)
                     /locus_tag="VGO32_04605"
     CDS             complement(922..2157)
                     /locus_tag="VGO32_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200066.1"
                     /translation="MPDTRGDVLCPTCGLWTPPAPFCTECGAALQVGLQESASGGGSD
                     VFRRGHEVEQPGSPWGQSERFEPEPEDQAARAAAAAAAGAAGAARVDHFAEIEPAAPE
                     PEWPSEPGLGGDAPPVRDETHPGAPMGAAAATAAAMAGADDDLTTEEPPAPKRRTKAK
                     AVGTAAAVGAAAGAAATGAAVTGAAPPKSTPPTPPIVEPPPAPPAGPPPVTYAGPPQD
                     GGDDGNSGVSGLVFVLFLGLGILALLGGAILGGVFDGGVAEASPTPSATASTPSPEPS
                     LTPSASASSAPSIAPSVAPTPTPVPASDGFIARAEPCDEQPSDPTCDNSGAVNNGHVW
                     ILVSFRHGQASDVIGVSVRDGSGSVVDQGSFPLNFCGAITDCAGYTFFEFSNLADGDY
                     DVVATRNGDEVATTAFTVE"
     gene            2222..2779
                     /locus_tag="VGO32_04610"
     CDS             2222..2779
                     /locus_tag="VGO32_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV7200067.1"
                     /translation="MRRINVVGSSGSGKTTTGAELARRLGVPLVELDSLSWEPNWVAA
                     PIDVLRERVAAAVSGETWVVDGNYGVARDLVWARADTVVWLDLSLWIVMWRVCSRTVR
                     RLVRREVLWNGNRESMRTTLSRDSIILYALTEFRRRRRELSSALAAQPHLGVVRLTTA
                     GDVERFLAAIPTPASMQPGTRRAQR"
     gene            complement(2805..3467)
                     /locus_tag="VGO32_04615"
     CDS             complement(2805..3467)
                     /locus_tag="VGO32_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEV7200068.1"
                     /translation="MEIQVRCFATLREASAARTALTMAPPATVAAAWSEMESRFPALV
                     PHRAFTQPARNGVAVGWDEPLADGDDVAFLPPVSGGSDPTLIGLSADQIDVAALERVV
                     DRSHGAVVTFVGRARNRSDDGREVTALEYEAYPEMADATLRVIANEVTTRWPDCVVAV
                     VHRTGTVPIGEAAVAIVTGAPHRGDAYDANRYVIEAIKDRLPIWKLEQFTDGSRWKRP
                     GA"
     gene            complement(3471..4277)
                     /locus_tag="VGO32_04620"
     CDS             complement(3471..4277)
                     /locus_tag="VGO32_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200069.1"
                     /translation="MALGETQPLAVEALPFVNWIFWGTLAGGTLLAVGLTQWLGGTTI
                     GYRRFMAATIAIGAGIWLLSELTVHAGPTTESFSTLRRTLVVASAALALGYLLAVVIT
                     TFPERVGTALALGGGVVSILALLTIPAVASGEQVLFAAGLVSAAVALGSVTAGMLLGH
                     WYLVTPKLSPDPIRRLIWVLIGALVLQGLLVAWSVVIAWGVIEPVAWLTGLRFFVGVV
                     FPLAIAILALRATTAPSMQSTTGLLYIGLAAVMAGTIGAASLVYLGWGVV"
     gene            complement(4277..4633)
                     /locus_tag="VGO32_04625"
     CDS             complement(4277..4633)
                     /locus_tag="VGO32_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEV7200070.1"
                     /translation="MTQAATRFVRILPAANLRDGDLIPVVIDGQPVVLVRHLGEFFAV
                     QNNCTHRDYPLTEAGFDVRDGVLVCAWHNGCFDVRTGAAVALPATEPVETFPTRVVDE
                     WVEIGLTGSLLAGTVS"
     gene            complement(4634..6076)
                     /locus_tag="VGO32_04630"
     CDS             complement(4634..6076)
                     /locus_tag="VGO32_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7200071.1"
                     /translation="MTTEVRAQSVPIYVAGQFVEAGSPLEIRNPATGDLVATTWQAGA
                     AELDTATEAAVTAFEQTRRLASFERRDALQHVADCIARDADELAELLTRESGKPIRDA
                     RGEVARGSLTFRTAAEEALRINGEWLPLDWTAANRGRSGIVRRYPIGPVAGISPFNFP
                     LNLAAHKVAPAIAAGCSIVLKPPSKDPLVMLRIAQYLDQTSLPKGAVSILPMDRPTGD
                     RMVADDRFKLLSFTGSPGVGWKMKAEAGRKKVVLELGGNAGVIVDETADLDWAVARLV
                     FGAFAYAGQVCISVQRIYVVESIYAEFERRFVEKVREVKMGDPLDPASDLGPMVDEAA
                     VSRTDSWVQEALADGARALTGGRAEGLFYEPTILVDVPRDARVCGEEVFAPVVNLFPV
                     PDFAAALREINDSRFGLQCGLFTSSLENTLAAHDELEVGGIIVNDVPTWRTDAMPYGG
                     VKDSGLGREGLRWSIEDMTEPRLLAFARPL"
     gene            6138..7085
                     /locus_tag="VGO32_04635"
     CDS             6138..7085
                     /locus_tag="VGO32_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEV7200072.1"
                     /translation="MNPVFLFLEVAATVLFVGAAAAALRRGRLPFLELISAAAFGLLL
                     EEGDQLIFQTYHYSPDWVLVLDRAPLVIGLTWALLIAGAMRITDALGVRRWTAPFVDA
                     VLVIMLDLAFDAVAIRMGMWTWVGIEPDQGWFGVPAGNFYAWLFVTLGFSLLSRWIRD
                     FSVRRPSFEWLQLGVPVPAFAILITSIIPFAVIMELTGAEPGGGLWLFALTLAVFVAI
                     AGVGTFGPKRQAPDGRAKAIVDLRLAFFTRFSMHGFFLVALLVMGLATQLPILLVTSV
                     GLFFADVAISRLVEQRRIRPAIGVPAGALTPAPAPVRDS"
     gene            7082..>8239
                     /locus_tag="VGO32_04640"
     CDS             7082..>8239
                     /locus_tag="VGO32_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV7200073.1"
                     /translation="MTAPASGTADADEAIQHLRELIRIPSVNPPGDPTLAGGRDSTGG
                     ETAAARYCAEVLTAAGISAEVLETAPGRGSCFARLPADPAVRDPEPPLILLGHVDVVP
                     VEAEAWTRDPFGGELVDGVVWGRGAVDMKDMVAMELAIMVALHRSGRPLRRDLIFAAV
                     ADEEAGGSHGAQHWVEARPDLFSSAAGPAAAALNEVGGYSITLGDRRAYAIQVAEKGI
                     IWTRLHATGMPGHGSMPHDQNPAVRLAAAVTRLAEAPPTGEPPAVVRDFFAGLGLGAV
                     TDLVPGDRPGAVALLEQLVSDPTMRRSLGAMLRDTVTPTVIRVGSKMNVIPGFGTAEV
                     DVRTLPGTDQAAFADRLRALGGPEVEVEIVMSLPAIEAPADAPIVDLMRAAL"
BASE COUNT         1156 a         2901 c         2943 g         1239 t
ORIGIN      
        1 gcgctcgcgg cgcgacgacc tgatgtcgtg atcctgggcg cccccctgga cgatggcgtg
       61 agccaccggc cgggcgctcg attcgggccg cgcgcgatcc ggaccgcctc ctaccactca
      121 ggcgacatcc actcgctcca ggtgggcgtg gacccgttcg actggctgga cgtggtggac
      181 ggcggcgacg ccccggtggt cccgggccgg ctcgagcgtg gccacgcggt gatccgggcc
      241 cgggtgctcg agatcgcgcg gacgggtgcg atcccgatcg tgctgggcgg cgaccactcg
      301 atcacgtacc cgtcggccag cgcggtcgcg gaggcggtgg ctcccaggcg actggggatc
      361 gtgcatttcg acgcccacgc cgacaccggc gacaacacgt ggggaaacct cctgtcccac
      421 gggacaccga tgcggcggct catccagtcg ggcgccgtgg ctggtcgcaa cttcgtccag
      481 gtcgggctcc gcggctactg gccgccggca gagacgctgg cctggatgcg cgagcaggag
      541 atgcgctggc atctcatgac cgagatcgag gcacgcggcg ccgaggccgt cgtccacgac
      601 gcgatcgccg aagccctgga cggtcctgat gtcgtgtacc tctcggtgga catcgacgtc
      661 atcgatccgg ggatggcgcc ggggaccggg acccccgagc cgggtgggat gctgacccgc
      721 gagctgctcc gcgcgatccg gcaggtcgtc ggcgccgtgg acctggtggg gatggacgtg
      781 gtcgaggtgg ctcccgccta cgacgtgtcc gacgtgaccg ccgccgccgc ccatcggtgc
      841 gtgctcgagg cgatcagtgc gctcgccgga aggcggcgcg atgcccggga gtcggctcgg
      901 ccgaccgtcc gctgaacgcg gctactcgac cgtgaatgcg gtcgttgcga cctcgtcccc
      961 gttgcgggtg gccactacgt cgtagtcgcc gtcggccagg ttgctgaatt cgaagaacgt
     1021 gtagccggca cagtcggtaa tggccccgca gaagttcagg gggaagcttc cctggtcgac
     1081 gactgagccc gatccgtccc ggacgctgac cccgatcacg tcagacgcct gtccgtggcg
     1141 gaagctgacc aggatccaga catggccgtt gttcacggcg ccgctgttgt cgcaggtcgg
     1201 gtcgctcggt tgctcgtcgc acggctcggc gcgggcgatg aagccgtccg aggccgggac
     1261 cggggtcggg gtcggcgcca cgcttggcgc gatcgacgga gccgatgagg ccgaggccga
     1321 tggcgtcagc gacggctccg gagagggcgt gctcgcggtg gcgctcgggg tcggcgatgc
     1381 ctcggcgacc ccgccgtcga agacgccgcc caagatggcc ccgccgagga gggcgaggat
     1441 gccgaggccg aggaagagga cgaagacgag accactgacc ccgctgttgc cgtcgtcgcc
     1501 accgtcctgg ggcggtccgg cgtaggtgac cggtggcggc ccggccggcg gagcgggcgg
     1561 cggctcgaca atcggcggcg tcggaggcgt ggatttcggt ggggcggcgc ctgtcacggc
     1621 ggcgcctgtc gcggcggctc cggccgcggc cccaaccgcg gcggccgtcc cgactgcctt
     1681 ggccttggtc cggcgcttgg gggcaggagg ctcttcggtc gtcaggtcgt cgtcggcccc
     1741 ggccatcgcc gccgcggtcg ccgcggcggc gcccatcggc gcgcccgggt gcgtctcgtc
     1801 cctgaccggc ggcgcatcgc cgccgagtcc gggctcggac ggccattccg gctccggtgc
     1861 agccggttcg atctccgcga agtgatcgac ccgggctgcg ccggcggccc cggccgcggc
     1921 agcggcggcg gcccgcgcgg cctggtcttc gggctctggc tcgaatcgct cggactggcc
     1981 ccacggggaa cccggctgct ccacctcgtg gccccgccgg aagacgtccg accctcctcc
     2041 ggaggcagat tcctggaggc cgacctggag ggccgcgccg cattcggtgc agaacggcgc
     2101 cggcggcgtc cagaggccgc aggtcgggca gagcacgtcg ccccgtgtgt caggcatcgg
     2161 cgcgcattgt gccccgagca gtccccccgg cgccaccgag cgatcggtat cctcggcgat
     2221 cgtgaggcga atcaacgtgg ttgggagcag cggttcgggg aagaccacga ccggggcgga
     2281 actcgcccgg cgcctcggcg tgccactcgt cgagctcgac tcgctgtcct gggagccgaa
     2341 ctgggtcgcc gccccaatcg acgtcctccg ggagcgagtt gctgccgccg tttctggcga
     2401 aacatgggtc gtggacggca actacggcgt ggcgcgggac ctcgtatggg cacgggccga
     2461 caccgtcgtc tggctcgatc tttccttatg gatcgtcatg tggcgggtct gttcacgcac
     2521 ggtcaggcgt ctcgtgcgcc gcgaggttct atggaacggc aaccgggaga gcatgaggac
     2581 gaccctcagc agggactcga tcatcctgta tgccctgacc gaattccgcc gccgaaggcg
     2641 cgaactttcg agcgcactag cggcgcagcc gcacctgggg gtcgtgcgct tgaccacggc
     2701 gggcgacgtc gagcgcttcc tcgcggctat tccgaccccg gcgtcgatgc agcccgggac
     2761 tcgacgagcc caacggtgac ggccaggatc gcgacgggtc ccgatcaagc ccccgggcgc
     2821 ttccaacggg acccgtccgt gaactgctcg agcttccaga tcgggagccg gtccttgatc
     2881 gcctcgatca cgtatcggtt cgcgtcgtag gcgtctccgc ggtgcggcgc cccggtgacg
     2941 atcgccaccg cagcctcgcc gatgggcacc gtcccggtcc tgtggacgac tgcaacgacg
     3001 cagtcaggcc atcgggttgt cacctcgttg gcgatgaccc gcagcgtggc gtccgccatc
     3061 tcggggtatg cctcgtactc gagcgcggtg acctcccgcc cgtcgtcgga gcggttccgg
     3121 gcccgtccga cgaacgtgac caccgcccca tgggatcgat ccacgacccg ctcgagggcg
     3181 gcgacatcga tctggtcggc actgagaccg atcagcgtcg ggtccgaccc accggacacc
     3241 ggcggcagga acgcgacgtc gtcaccgtcg gccaatggct cgtcccatcc gacggcgacg
     3301 ccattgcgcg ccggttgggt gaacgcgcgg tggggaacca gcgcggggaa gcgggactcc
     3361 atctcggacc aggccgccgc gaccgtcgcg ggtggcgcca tggtcagtgc ggtccgggcc
     3421 gcgctggcct cgcgcagcgt ggcgaagcag cgaacctgga tctccatcgg ctacaccacc
     3481 ccccagccga ggtacaccag cgaagcggcc ccgatcgtgc cggccatcac cgccgcgagg
     3541 ccgatgtaca gcagaccggt ggtggactgc atggacgggg cggtcgtcgc gcgcagggcg
     3601 aggatggcga tggccagcgg gaacacgacg cccacgaaga atcggagccc ggtgagccac
     3661 gcgaccggct caatgacccc ccatgcgatg accaccgacc acgcgacgag gaggccctgg
     3721 aggacgagcg ccccgatgag cacccagatc aggcggcgga tcggatcagg cgagagcttc
     3781 ggggtgacga gataccagtg gccgagcagc atcccggcgg tgaccgatcc cagggcgaca
     3841 gccgcggata ccaatccagc ggcgaacagg acctgctcgc cgctggcgac ggccgggatg
     3901 gtgagcaggg cgagaatgct gaccaccccg ccgcccagcg ccagcgcggt gccgacccgc
     3961 tcgggaaagg tcgtgatcac cactgccagg agatagccga gggcgagcgc cgcagacgcc
     4021 accacgagcg tgcgacgaag ggtggagaag gattcggtcg tcggtccggc gtgcacagtg
     4081 agctcggaca ggagccagat cccggcgccg atcgcgatgg tggcggccat gaaccgccga
     4141 tacccgatgg tcgtgccgcc gagccactgg gtcaggccga ccgcgagcag cgtgccgccg
     4201 gcaagcgtgc cccagaagat ccagttcacg aacgggaggg cctcgaccgc gagtggctgg
     4261 gtctccccga gcgccatcac gacacggttc cggccaggag ggatccggtg aggccgatct
     4321 cgacccattc gtcgacgact cgggtcggga atgtctccac cggctcggta gcgggcagcg
     4381 ccaccgccgc accggtccgc acgtcgaagc atccgttgtg ccaggcgcag acgaggacac
     4441 catcgcggac gtcgaagccg gcctcggtca acgggtagtc gcgatgggtg cagttgttct
     4501 ggacggcgaa gaactccccg aggtggcgga cgagcacgac cggctggccg tcaatgacga
     4561 ccgggatgag atccccgtca cgcaggttgg ccgcgggcag gatccggacg aagcgggtgg
     4621 cggcctgggt cactcagagt gggcgggcga aggcgagcag ccgtggctcg gtcatgtcct
     4681 cgatcgacca gcgcaggccc tcgcgaccga ggcccgaatc cttgacgcca ccgtacggca
     4741 tcgcgtcggt gcgccaggtg gggacgtcat tgacgatgat cccgcccacc tcgagctcgt
     4801 cgtgggcggc cagcgtgttc tccagcgagc tcgtaaacag gccgcactgg agcccgaatc
     4861 gactgtcgtt gatctcgcgc agcgcggcgg cgaagtccgg caccgggaac aggttcacga
     4921 ctggtgcgaa cacctcctcg ccgcagacgc gggcgtcgcg cggaacatcg accaggatgg
     4981 tcggctcgta gaacaggcct tcggctcgac caccggttag cgcccgtgcc ccgtcggcca
     5041 gggcctcctg gacccagctg tcggtgcgcg aaacggcggc ctcatcgacc atcggaccca
     5101 ggtctgacgc ggggtcgagc gggtcgccca tcttgacctc gcggaccttc tcgacgaagc
     5161 gccgctcgaa ctcggcgtag atcgattcca cgacgtagat acgctggacg ctgatgcaga
     5221 cctgccccgc gtaggcgaag gcgccgaaca cgagccgggc gaccgcccag tccaggtccg
     5281 cggtctcatc cacgatcacg ccggcgttac cgccgagctc gagcacgacc ttcttgcgcc
     5341 cggcttcagc cttcatcttc cagcccacgc ccgggctgcc ggtgaagctc aggagcttga
     5401 accgatcatc ggccaccatc cggtccccgg tgggccggtc catgggcagg atgctgaccg
     5461 cgcccttggg caggctcgtc tggtcgaggt actgggcgat ccggagcatg accagcgggt
     5521 ccttgctcgg cggcttgagc acgatcgagc agcccgcggc gatggcgggg gcgaccttgt
     5581 gggcggccag gttgagcggg aagttgaacg ggctgatccc ggccaccgga ccgatggggt
     5641 accgccgcac gatcccgctt cgtccccggt tggccgcggt ccagtccagc ggcagccact
     5701 ccccgttgat ccggagcgcc tcctcggccg cggtgcggaa ggtcaggctg ccgcgggcca
     5761 cctcgccccg ggcatcgcgg atcggcttgc cggattcgcg agtcagcagc tcggccagct
     5821 cgtcggcgtc gcgggcgatg cagtccgcca cgtgctggag cgcatcccgg cgctcgaagc
     5881 tggccagccg tcgcgtctgc tcgaatgcgg tcacggccgc ttcggtcgcg gtatccagct
     5941 cggccgcccc cgcctgccag gtggtggcca ccaggtcgcc ggtggccggg ttgcggatct
     6001 caagcggaga tccggcctcg acgaactggc cggccacata gatcgggacg ctttgtgcgc
     6061 gcacctcggt ggtcacgcgc gccattctac gaccgggatt tggccggtcg aacccgcccc
     6121 cctatcatcg agccgtcgtg aaccccgtct tcctcttcct cgaggtcgcg gccaccgtcc
     6181 tcttcgtcgg agccgccgcc gccgccctcc ggcggggacg cctgccgttt ctcgagctga
     6241 tcagcgccgc cgcgttcggg ctcctcctcg aggagggcga ccagctcatc ttccagacct
     6301 accactacag ccccgattgg gtgctcgtcc tggatcgggc gccgctggtc attggcctga
     6361 cctgggcgct gctgattgcc ggcgcgatgc ggatcaccga tgcgctcggc gtccgccgct
     6421 ggacggcacc gttcgtggac gcggtgctcg tgatcatgct cgatctcgcg ttcgacgcgg
     6481 tcgccatccg gatggggatg tggacgtggg tcggtatcga gcccgaccag ggctggttcg
     6541 gcgtcccggc cggcaacttc tatgcctggc tgttcgtgac cctgggcttc agcctgctga
     6601 gccgatggat ccgtgacttc tcggtgcgcc gaccgagctt cgagtggctc cagctgggcg
     6661 ttcccgtgcc ggcattcgcc atcttgatca cctcgatcat ccccttcgcc gtgatcatgg
     6721 agctgaccgg ggccgagccg ggtggcggtc tgtggctgtt cgccctcacg ctcgccgtgt
     6781 tcgtggcgat cgccggggtc ggcaccttcg ggccgaagcg ccaggcgccc gatggccgcg
     6841 cgaaggccat cgtcgacctc cgccttgcat tcttcacccg cttctcgatg cacgggttct
     6901 tcctggtcgc cctcctggtg atgggcctcg ccacccagct ccccatcctt ctcgtcacct
     6961 cggtcgggct cttcttcgcc gatgttgcga tttcccggct cgtcgagcag cgccggatcc
     7021 gcccagcgat cggcgtgccc gccggcgcgc tgacgccggc acccgcgccc gtccgcgact
     7081 cgtgaccgca ccggcgtcgg ggaccgcaga cgccgacgag gcgatccagc acctccggga
     7141 gctcattcgc atcccgtcgg tcaacccgcc cggtgacccg acgctcgcgg gtgggcgcga
     7201 ctcgaccggg ggcgagaccg cggcggcgcg ctactgcgcg gaggtcctga ccgcggccgg
     7261 catcagcgcc gaggtgctgg agacggctcc gggtcgcggc agctgcttcg cccgtctccc
     7321 cgcggatccc gcggtccgag atcccgagcc cccgctcatc cttctcggtc acgtggacgt
     7381 cgtccccgtc gaggccgagg catggacgcg tgatccgttc gggggcgagc tggtggatgg
     7441 cgtcgtgtgg ggccgcgggg cggtcgacat gaaggacatg gttgccatgg agctcgcgat
     7501 catggtcgcg ctccatcggt ccggtcgccc gctccgacgc gacctgatct tcgcggccgt
     7561 ggccgacgag gaggcgggcg gctcgcacgg cgcccagcac tgggtcgagg cgcggccaga
     7621 cctgttctcg tcggccgcag gcccggcggc cgcggccctg aacgaggtcg gcggctactc
     7681 gatcacgctc ggcgatcggc gggcgtatgc catccaggtc gccgagaagg gcatcatctg
     7741 gacccgcctc cacgcaaccg ggatgcccgg ccacggcagc atgccgcacg accagaatcc
     7801 ggcggtccgg ctcgcggccg cggtgacacg cctggcggag gccccaccca cgggtgagcc
     7861 gcctgccgtc gtccgcgact tcttcgcggg cctcgggctc ggcgccgtga ccgatctcgt
     7921 tccgggcgac cggccagggg cggtcgcgct cctcgagcag ctggtgtccg atccgaccat
     7981 gcggcgatcc ctgggcgcca tgctccgcga caccgtgacg ccgaccgtga tccgggtcgg
     8041 cagcaagatg aacgtgatcc ccgggttcgg cacggctgag gtcgacgtcc gtaccctgcc
     8101 cggcacggac caggccgcgt tcgcggaccg gctgcgggcg ctgggcggtc ccgaggtcga
     8161 ggtcgagatc gtcatgagcc tgcccgcgat cgaggctcca gccgacgcgc cgatcgtcga
     8221 cctgatgcgg gcggccctc
//
LOCUS       DASYGU010000169         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_131067, whole genome shotgun sequence.
ACCESSION   DASYGU010000169 DASYGU010000000
VERSION     DASYGU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131067"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VGO32_04645"
     CDS             <1..1740
                     /locus_tag="VGO32_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV7200074.1"
                     /translation="QTDVGPATAAAAASAGASTKPGVSPSSARPTPEDPPLILRRQRA
                     EPAAMAASSAAAGVAVPIVSPPPVTEEELESSEEAHFAEVGEREAVDAALEEAAQNWT
                     LPDISLLADAPGQGPAQMDLTAKGQLIKNTLASFDIGVKVARIQEGPVVTQYALDVDP
                     GIKLSRIQGLADNLALALSARSIRIQAPIPGEPYVGIEIPNAAFDLVTLKEVLGSKAL
                     QTLAAEGELAFALGQDVAGQPVAADLGRMPHLLIAGATGSGKSVCVNALICSLVMRAK
                     PDEVKLILIDPKRVELAQYRGLPHLLTEVIVDTEKAVNALKWTVGTMESRYHEFAQRG
                     VRNIAGFNAELRSDEPRMPYIVVVIDELADLMMMSAYEVEATITRIAQLARATGIHLV
                     VATQRPSVQVVTGLIKANIPSRIAFAMTSGIDSRTILDATGAEDLLGRGDMLYLPIDA
                     PRATRLQGVLVTDPEIEALTKFWKGQGAPKYRTEITAARREHRNGDGTNEDGDADPLL
                     RQAVDIVRRSDKASASLLQRRLRVGYARAARILDQMEDRGIVGPADGSRFREVLITPD
                     GWGGEIGPGEDEA"
     gene            1737..3059
                     /locus_tag="VGO32_04650"
     CDS             1737..3059
                     /locus_tag="VGO32_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7200075.1"
                     /translation="MTQAGRSLGQTLRAAREGKRWDIARAERDTRIRARYLIALEAGD
                     YRDLPSPVYTKGFVRNYALYLGLDPEWCLDLYRLEANPTEKARPVITDPTAKTVEAKG
                     GALLTTSRVIRVGLLVLVAALVAYIGYQFVTFAGTPELTLTDPASDLAAYGGTSYVLR
                     GETVADAQITVDGLRENPTATANGTGDVSIRVELLPGSNVITLVATDPLTGRQSSPVT
                     RTITVTLDLATPLPGGAPNLSSPEADAVISGGVTVAGSVAPGASVTVRAALIGAPNPS
                     FEIVNLAGPSVAVPPWSPVALDPVSVSADLAGQFSVPLELGIGTWELRVSATGATPSP
                     APSGAVPTETIRRVTVVSAAGLSGTLEVRDGPTAVELDEDGVPRAGVSGQDQAAGTRI
                     TLSADERIRLRVGDAGRVHLSLNGYVISPMGDAGATVEWAIRALVPGG"
     gene            3070..>3516
                     /locus_tag="VGO32_04655"
     CDS             3070..>3516
                     /locus_tag="VGO32_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV7200076.1"
                     /translation="MADSSFDVVSDFDQQELVNALDQVRREIATRYDFKNSRCTLELG
                     KTDITLVADDDFRAAAVKDLVQSKAVKRGLSLKIFDWGKAEPAAGGTVRQVIALKRGL
                     TADQAKELSKHVRDHFPKVKVSIQGDALRVISPKKDELQGVIADLRA"
BASE COUNT          547 a         1295 c         1194 g          480 t
ORIGIN      
        1 cagaccgatg tcggcccggc caccgcggcc gccgccgcga gcgcgggcgc atccaccaag
       61 ccgggcgtat cgccttcatc cgctcgcccg acgccggagg acccgccgct catcctccgc
      121 cggcagcggg ccgagcccgc ggcgatggcc gcctccagcg cagcggccgg cgtggccgtg
      181 cccatcgtca gtccgccgcc ggtcaccgag gaggagctcg agtcgagcga ggaagcgcac
      241 ttcgccgagg tgggggagcg ggaggcggtg gacgccgctc tcgaggaggc ggcgcagaac
      301 tggaccctgc ccgacatctc gctcctggcc gacgcgccgg gccagggccc ggcccagatg
      361 gacctcacgg ccaaaggtca gctgatcaag aacacgctgg ccagcttcga tatcggcgtg
      421 aaagtggccc gtatccagga gggcccggtc gtcacccagt acgcgctcga cgtggacccc
      481 ggcatcaagc tgtcgcggat ccagggcctc gccgacaacc tcgccctcgc cctgtccgcc
      541 cgatcgatcc ggatccaggc ccccatcccc ggcgagccct acgtcgggat cgagatcccg
      601 aatgcggcct tcgacctggt tactctgaag gaggtgctcg gctcgaaggc gctccagacc
      661 ctcgccgccg agggcgagct ggccttcgcg ctgggccagg acgtcgccgg ccagccggtg
      721 gccgcggacc tgggccggat gccgcacctg ctcatcgcgg gcgccaccgg gtcgggcaag
      781 agcgtgtgcg tgaacgcgct catctgctcg ctcgtcatgc gcgccaagcc cgacgaggtg
      841 aagctcatcc tcatcgaccc caagcgcgtg gagctggccc agtaccgcgg cctgccgcac
      901 ctcctgaccg aggtcatcgt cgacaccgag aaggcggtca acgccctgaa gtggaccgtc
      961 ggcacgatgg agagccggta ccacgagttc gcccagcgcg gcgtccggaa catcgccgga
     1021 ttcaacgccg agctgcgctc ggacgagcca cggatgccat acatcgtggt cgtcatcgac
     1081 gagctggccg acctcatgat gatgagtgcc tacgaggtcg aggcgaccat cacccgcatc
     1141 gcccagctgg cccgggcgac cggcattcac ctcgtggtcg ccacccagcg cccatcggtc
     1201 caggtcgtga ccggcctgat caaggccaac atcccatcgc ggatcgcgtt cgcgatgacc
     1261 agcgggatcg acagccggac cattctcgat gcgaccggcg ccgaggacct cctgggccgc
     1321 ggcgacatgc tctacctgcc catcgacgcg ccgcgcgcga cgcgcctcca gggcgtgctc
     1381 gtcaccgacc ccgagatcga ggcgctcacc aagttctgga agggccaggg cgcgccgaag
     1441 taccggaccg agatcacggc cgcccgccgc gagcatcgga acggcgacgg gaccaacgag
     1501 gacggcgacg ccgatccgct cctccgccag gcggtggaca tcgtccgtcg ctcggacaag
     1561 gcctcggcct cgctcctcca gcgccgactg cgggtgggct acgcccgcgc ggcccgcatc
     1621 ctcgaccaga tggaggaccg gggcatcgtc ggccccgccg acgggagccg cttccgcgag
     1681 gtgctcatca cgcccgatgg ctggggcggg gagatcggtc cgggtgagga tgaggcatga
     1741 cccaggccgg ccggtccctg gggcagacgc tccgggcggc ccgcgagggg aagcgctggg
     1801 acatcgcccg cgctgagcgc gacacccgga tccgcgcccg ctacctgatc gccctcgagg
     1861 cgggtgacta tcgagacctc ccgagcccgg tctacaccaa gggcttcgtc cgcaactacg
     1921 ccctgtatct gggcctggac cccgagtggt gtttggatct ctatcggctg gaggccaacc
     1981 cgaccgagaa ggcgcgcccg gtcatcaccg atccgacggc caagacggtg gaggccaagg
     2041 gcggcgccct gctcacgacc tcgcgcgtca tccgcgtcgg cctgcttgtg ctggtggccg
     2101 ccctggtggc ctacatcggc taccagttcg tcacgttcgc cggcacgccg gagctgaccc
     2161 tgaccgatcc cgcctcggac ctcgccgcct acggcggaac gtcatacgtg ctccgcggcg
     2221 aaacggtggc cgacgcccag atcacggtcg acggcctgcg cgaaaacccg accgccaccg
     2281 ccaatggcac gggcgacgtc tcgatccggg tcgagctcct gcccggctcg aacgtcatca
     2341 ccctggtcgc caccgacccg ctgaccggtc gccagtcgag cccggtgacc cgcacgatca
     2401 cggtgaccct cgatctggcg acgcccctgc ctggcggcgc tcccaacctg agctcgcccg
     2461 aagcggacgc ggtcatctcg ggcggggtca ccgtggccgg atcggttgcc cccggtgcct
     2521 cggtcacggt ccgggccgcg ctcatcggcg cccccaatcc gagcttcgag atcgtgaacc
     2581 tggccggtcc atcggtggcc gtcccgccgt ggagccccgt tgccctcgat ccggtatcgg
     2641 tcagtgccga cctggccggg cagttcagcg tgccactgga gctggggatc ggaacctggg
     2701 agctgcgcgt gtccgcgacc ggcgcgacac ccagcccggc gcccagcggc gccgtaccga
     2761 ccgagaccat ccgccgcgtg accgtggtct ccgcggccgg cctgtccggc acgttggagg
     2821 ttcgcgacgg gcccaccgcc gtggagctgg acgaggacgg cgtgccacgg gccggcgtct
     2881 cgggccagga ccaggcggcg gggacgcgga tcacgctcag cgccgacgag cggatccggc
     2941 tccgggtggg ggatgcgggt cgcgtgcacc tctccctgaa cggatacgtg atctcgccca
     3001 tgggtgatgc cggggcgacc gtcgagtggg cgatccgcgc cctcgtgccg ggcggctagc
     3061 attagggaca tggccgactc ctccttcgat gtggtcagcg acttcgacca gcaggagctg
     3121 gtgaacgccc tggaccaggt ccggcgcgag atcgcgaccc gatacgactt caagaactcc
     3181 aggtgcacgc tcgagctggg caagaccgat atcaccctgg tcgccgatga cgacttccgc
     3241 gccgccgcgg tcaaggacct cgtccagtcc aaggccgtca agcgcggcct cagcctgaag
     3301 atcttcgatt ggggcaaggc cgagccggcg gccggcggca ccgtccgcca ggtcatcgcg
     3361 ctcaagcgcg gcctgacggc tgaccaggcc aaggagctgt cgaagcacgt tcgggaccac
     3421 ttccccaagg tcaaggtctc gatccagggc gacgcgctgc gggtcatctc gcccaagaag
     3481 gatgagctcc agggggtcat cgccgatctc cgagcg
//
LOCUS       DASYGU010000170         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1622951, whole genome shotgun sequence.
ACCESSION   DASYGU010000170 DASYGU010000000
VERSION     DASYGU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622951"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VGO32_04660"
     CDS             complement(<1..376)
                     /locus_tag="VGO32_04660"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200077.1"
                     /translation="MTDHPAGPLPAISEVATDELVAWFGDHGEPAYRARQVMAGVAAG
                     RATDFEALTDLPKRLRTTLSDAFRFSSIGDTHVTGTDQSLTEKALHELADGQRIESVF
                     MRYPGRGASAARTTICISSQAGC"
     gene            complement(369..1070)
                     /locus_tag="VGO32_04665"
     CDS             complement(369..1070)
                     /locus_tag="VGO32_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEV7200078.1"
                     /translation="MFFDPLYLVLVVVMLAISGFAAWRVRSTYARWSKVDSGIDLSAY
                     DFARRLLDRQDLTNVQIEPTPGALTDHYDPRSKTLRVSQTVASTGTGGGPQLSVAAAA
                     VIAHEVGHALQDRQRDPWLTARSVIVPVASFGSSIAPWLIIAGILLQLTGLAIVGLIG
                     FAAAFAFTLITLPVEIGASGKALAFVEGLGMVGERQDGARAVLKAAAWTYVAAALTAL
                     LQFLYYFSLVMGRRD"
     gene            complement(1143..2081)
                     /locus_tag="VGO32_04670"
     CDS             complement(1143..2081)
                     /locus_tag="VGO32_04670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV7200079.1"
                     /translation="MLGRIAFLGSGAFGLPLLERLPALASDLLVVSRPDRPAGRGLHD
                     RASPIAETARGLDLALLTPTGLRSDQARQAITDFRPDGLLLAAYGELVPRDLLDLAPR
                     PPLNVHPSLLPRHRGAAPVAATILSGDDVGGVSLMIMVPQLDAGPIVGQWRVPLTGRE
                     EAPQLEAQLGALAADVVPAVLDAWARNELEPVPQDEAEATYRPSFTRADGWIPWATSA
                     EQIDRQVRALQPWPGAWTTLDGRRLHIRRAHPLPGLPGLPIGSLMPGPPVVACGLGAL
                     ALDIVQPEGRPPMAADAWRNGLSRDHVLLGGDAPPD"
     gene            complement(2081..>2344)
                     /locus_tag="VGO32_04675"
     CDS             complement(2081..>2344)
                     /locus_tag="VGO32_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV7200080.1"
                     /translation="TVDWEGCLSIPGFVAEVERSEKVTVQARDRNGREFRVKGDELFA
                     RALQHEIDHLNGVLYIDYLESLEELVRVSEASDEVREEAAAGI"
BASE COUNT          358 a          803 c          858 g          325 t
ORIGIN      
        1 cgcagccggc ctggctcgag atgcagatcg tggtccgcgc cgccgatgcg cctcgccccg
       61 ggtagcgcat gaagaccgac tcgatccgct gtccgtcggc cagctcgtgg agcgcctttt
      121 cggtgagcga ctggtctgtg ccggtgacgt gcgtgtcgcc gatgctcgag aagcggaagg
      181 cgtcggacag ggtcgtccgc agtcgctttg gcagatcggt cagtgcctcg aagtcggtgg
      241 cgcggccggc cgcaacacct gccatcacct ggcgggctcg gtaggcgggt tcaccatggt
      301 cgccgaacca ggcgaccagc tcatcggtcg cgacctcgct gatggccggc agcggcccag
      361 cggggtggtc agtcacggcg acccatgacc aggctgaagt agtacaggaa ctggaggagc
      421 gcggtcagcg cggcggcaac gtaggtccat gcggcggcct tgagcaccgc gcgggcgcca
      481 tcctgtcgct cgcccaccat gccgagtcct tcgacgaagg cgagagcctt gccactggcg
      541 ccgatctcga ccggcagggt gatcagggtg aacgcgaacg cggccgcgaa gccgatgagg
      601 ccgacgatcg ccaagccggt cagctggagg aggatgccgg cgatgatcag ccacggcgcg
      661 atcgacgagc cgaagctggc caccggcacg atgaccgagc gagcggtcag ccacgggtcg
      721 cgctgccgat cctggagcgc gtgccccact tcgtgggcga tcaccgccgc cgcggccacc
      781 gacagctgcg gcccgccacc ggtcccggtg ctggccaccg tctggctgac gcgaagggtc
      841 ttcgatcgcg ggtcgtaatg gtcggtgagt gcgccggggg tgggctcgat ctggacgttg
      901 gtgaggtcct gccgatcgag gaggcggcgt gcgaagtcgt acgcgctcag atcgatgccc
      961 gaatcgacct tcgaccagcg ggcatacgtc gaccgcacgc gccaggcggc gaagccgctg
     1021 atcgcgagca tgacgaccac cagaacgaga tacagggggt cgaagaacat cgtcaggtgc
     1081 cacctccagg caccgcggcc gagtatagga caagccgacg ccgggccaga aatcgcccta
     1141 tgtcagtccg gcggcgcgtc cccgccgagc agcacgtggt cccggctcag cccgttccgc
     1201 caggcgtcgg ccgccatcgg cgggcgtccc tcgggctgga cgatgtcgag cgccaatgcg
     1261 ccaagtccgc aggccacgac cggcggtccg ggcatcagcg atccaatggg caggcccggc
     1321 aggccgggca gtgggtgcgc ccgtcggatg tggaggcgcc gaccgtcgag cgtggtccac
     1381 gctcctggcc agggctggag agcgcgcacc tggcggtcga tctgctccgc gctggtcgcc
     1441 cacgggatcc agccgtcggc gcgggtgaac gacggtcgat aggtggcttc cgcttcgtcc
     1501 tgggggaccg gctccaactc gttccgagcc caggcgtcca ggaccgccgg cacgacgtcc
     1561 gccgcgagcg ccccgagctg ggcctcgagc tgcggcgcct cctcccgtcc ggtcagcggc
     1621 acccgccact gcccgacgat cggcccggcg tcgagctgcg ggaccatgat catcaggctc
     1681 acgcccccca cgtcgtcgcc ggacaggatg gtggcggcca ccggcgcggc gccgcggtgg
     1741 cgcggaagga gcgacgggtg gacgttcagc ggcggtcgcg gagcgaggtc cagcaggtcg
     1801 cgcgggacca gctccccgta ggccgcgagc agaaggccgt ccggacggaa gtcggtgatc
     1861 gcctgacgcg cctggtcgga tcgaagtccg gttggggtga ggagcgcgag gtcgagcccg
     1921 cgggcggtct cggcaatcgg tgatgcccgg tcatggaggc cccgtcctgc cgggcggtcc
     1981 ggtcggctga cgacgaggag gtcgctcgcc agcgcaggca ggcgttcgag gagtgggagg
     2041 ccgaacgcgc cgctgccgag gaaggcgatt cggcccagca ctagatcccg gccgccgcct
     2101 cttcccgcac ctcgtccgag gcctcgctga cgcggacgag ctcctcgagc gactcgaggt
     2161 agtcgatgta caggacgccg ttcaggtgat cgatctcgtg ctggagcgcc cgcgcgaaga
     2221 gctcatcgcc cttcacccgg aactcgcgcc cgttccggtc gcgggcctgg acggtcacct
     2281 tttccgagcg ctcgacctcg gccacgaagc ccgggatcga gaggcaccct tcccagtcga
     2341 ccgt
//
LOCUS       DASYGU010000171         8603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_79536, whole genome shotgun sequence.
ACCESSION   DASYGU010000171 DASYGU010000000
VERSION     DASYGU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79536"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..410
                     /locus_tag="VGO32_04680"
     CDS             48..410
                     /locus_tag="VGO32_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200081.1"
                     /translation="MRKTRVLIALPLLAVVLSACSSSTYSEAEQAGFIRDGIQQYAAA
                     PQPEWFDSFQEVAVERGVASIQTSLGPDDGDLAATMCTDISAVSLDADGQPMGVSTLR
                     IYGTESVSLAECGGPGGG"
     gene            complement(413..1615)
                     /locus_tag="VGO32_04685"
     CDS             complement(413..1615)
                     /locus_tag="VGO32_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7200082.1"
                     /translation="MPGRPSILICAPDEALRNALRSALSTSDQDVQEVATPAEAVAAL
                     GAGDIDLVIAEGLTATGAIGSLRAARADDGVPILVVAPPGDVEARIAFLEAGADDVIE
                     GGFARRELEARVHALLIRAGAVAPGPSSPGAGQMIAFFSPKGGVGTTALAVNCAALLA
                     SGGANAEVVGSRVVLVDLDLQFGQVATHLNLSPKLDFARLAVDDIARTDPEALAGYLT
                     AHDSGLSVLASPTSPDSAAGISVEEVEQVIGTLRTMFDFVVVDCGTRLDPRSIWVIEQ
                     AQESVLVVFPELGALRAMSDLMQFLADAAILQGRTHFVVNHISARELLKTRDVESLLR
                     ARPTAEIPFADVDMIRSVNEGSPIVLSKPSSPAGLAIQRLVAALVGAELRSTTRPRAP
                     RRALFSRG"
     gene            1803..2324
                     /gene="rpsF"
                     /locus_tag="VGO32_04690"
     CDS             1803..2324
                     /gene="rpsF"
                     /locus_tag="VGO32_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV7200083.1"
                     /translation="MRRYELMLLFRPDLEDDQLQSAVERVTRAIVNAGGSLSKISPWG
                     KRRLAYPIQHHRDASYFLIHFDIEPAAVREIERGILISEEILRHLVVVLEPRQRRDGE
                     GEMDGETEGAAVGEIDGLAPLPVSDDEPTAQALPEPQETDEPREAAATREDEPDETDV
                     VVEDVNAPEEVAG"
     gene            2321..2785
                     /gene="ssb"
                     /locus_tag="VGO32_04695"
     CDS             2321..2785
                     /gene="ssb"
                     /locus_tag="VGO32_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV7200084.1"
                     /translation="MTLNKMMIIGNLGADPELRYTPGGKAVTNLRVAVNDRHRGADGE
                     WVEETQWFRVELWDQAAERAAERFRKGNKVYAEGQLRVREYEGNDGQKRSSVEIRFAR
                     VISLERRDPGEGFGIGDAASDAPPAAVPRGAAQTVPSDGPATETVDLDDIPF"
     gene            2804..3082
                     /gene="rpsR"
                     /locus_tag="VGO32_04700"
     CDS             2804..3082
                     /gene="rpsR"
                     /locus_tag="VGO32_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV7200085.1"
                     /translation="MPSPARPRRRESTADYYRDRRRRKVCNFCVDKVDAIDYKEVNRL
                     RRYLSERAKIEPRRKTGTCARHQRMLTVALKRARHVALLPIAPQHVRP"
     gene            3124..4224
                     /locus_tag="VGO32_04705"
     CDS             3124..4224
                     /locus_tag="VGO32_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEV7200086.1"
                     /translation="MDVIVFIAALAIILLGAELFTNGIEWFGHRLNLGEGAVGSVLAA
                     VATALPETLIPVIAIIGPLLTGNEATAGSEEVGVGAILGAPFTLATLAMFITGLAVLI
                     SARNGGRSTDMKVNVNVMAQDVIFFLVSYTIAIGAAFLPEEWRWADFAVVAVLTLVYV
                     FYVRAHFREKGEGEAADLAQLHATRLPGMSGGVALQEAEGEGGAALEEGGLPRMWIIV
                     LQTIVALACIILGARIFVTAVEHISAGIGFDPRLFALIVAPIATELPEKFNSVLWVRK
                     GKDTLAMGNITGAMVFQACFPTAIGILFTSWAFTAENALSFASAGAAILATLLILGPM
                     LRSRRLSAAALLIGGPIYLIYILAVIFLPSGG"
     gene            4308..5267
                     /locus_tag="VGO32_04710"
     CDS             4308..5267
                     /locus_tag="VGO32_04710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV7200087.1"
                     /translation="MKQETSTGVQPLPRSTAGLWAYFRGELVPLDDARISVTTHAFNY
                     GTAVFEGVRAYWNPEQEQLYGLDLLAHFQRLHRSAPILQLELRLSPEELVEITCEMLR
                     RSEMREDAYIRPILYKSTEAVGVRLHNLDTDLVIFTLPFGKYIDTEGGIRAQVSSWRR
                     NDDNAIPARAKITGGYVNGALAKSDAQLNGYDEAIMLTQDGHVSEGSAENLFIVRDGA
                     LITPSITDNVLEGITRRHIMEVAGDLGIGVVERVMDRTELYQADEVFLCGTGAQLAPV
                     IEIDNRPIGGGTPGPITRRLHTIYFDAVRGRSDTYRGWLTPVY"
     gene            5302..6594
                     /locus_tag="VGO32_04715"
     CDS             5302..6594
                     /locus_tag="VGO32_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200088.1"
                     /translation="MTAATGPVIVSLARDARHALALPGLLVLVGVGIAAAGIGLGLAL
                     SIISLVALAAVVGIALAGIGAWMAIRIRSLKLAVEVDYLHLTGRGVDRRYHLAHGSLS
                     RVATSGPAGARLRTRPTSLGWGVGRSTLGSGERIDVIRLGATPSVVLIPTERGRVALA
                     AAVEADLIQALMGAAQTRAEREPTPAVVAAASVAPAPVAAAAIVAPPIARPPEPEAPR
                     RPLTGIERMALEEQMAEEHGAAYGGAPREQAMASFSPATYPPATYPPATYPPATPVVP
                     ATPPLADAPVVLPTPVSAAALPAIAATTAAPVVATTVSRTASTRSWPLAVPRGLPRRR
                     PLRPMAWRASRPTLVREIALLGVPLLMAGVVWFVAALVGATGEGQGLDPLSFALLLCG
                     PVAALAIFLARTRWPRLAGLSSLAAVFCLALVARAVIG"
     gene            6952..>8603
                     /locus_tag="VGO32_04720"
     CDS             6952..>8603
                     /locus_tag="VGO32_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7200089.1"
                     /translation="MSPGLDVPAVEREVAERWRAERTAERYLVRNAESDTRYSFLDGP
                     MTANNPMGVHHAWGRTYKDLYQRYHTMLGDRQRYQNGFDCQGLWIEVEVERELGFTNK
                     RDIEAYGIDKFVEACFARVQRFADRITEQSKRLGYWMDWDNSYFTNSDENNYTIWRFL
                     QRCQERGLLYRGHDVMPWCPRCGTGLSNMEIATEGYRELSHLSLTFRLPITSPGHGGE
                     SLLVWTTTPWTLSSNVAAAVHPDLVYDLVESPGGERWWVSRGSRARVAPAAPVVGEAL
                     GKDLLGLTYDGPFDELPVQAGVTHTVIAWDEVTDDEGTGIVHIAPGCGEEDFALAKRD
                     TLAVLDPIDEFGVFKEGYGWQTGRFAGATPDAADDLAPAVAAELEAKGLLVARETYRH
                     HYPVCWRCNTQLLFRLVDEWFIAMDPLREPISAVTRAVQWVPDGIGLEERELDWLKNM
                     RDWMISKKRYYGLALPFWVCDACEAWEVIGSKDELRERAVAGWEAFEGHSPHRPWVDA
                     VEIACQACGGRSRRIRDVGNPWLDAGIVAYSTLKWDTDPEYWA"
BASE COUNT         1326 a         2977 c         2971 g         1329 t
ORIGIN      
        1 ggtcggcgca tcggtctatc cttggcgccc gccctgtgag gattgacatg cgcaagacgc
       61 gggtcctgat cgcgcttcca ctgctggcag tcgtgctgag cgcctgctca tcgtccacct
      121 actcagaggc cgagcaggcc ggcttcattc gtgacggcat ccagcagtac gcggccgcgc
      181 ctcagccgga gtggttcgac tcgttccagg aggtcgccgt cgagcgtggc gtggccagca
      241 tccagaccag cctgggtccg gacgatggcg acttggccgc cacgatgtgc actgatatat
      301 cggcggtctc gctggatgcg gacgggcagc cgatgggtgt ctcgaccctg cggatctacg
      361 ggaccgagag cgtgagcctg gccgagtgcg gcggcccggg gggcggatag acctagccgc
      421 gactgaagag ggctcgccgc ggcgcgcgag gtcgcgtcgt cgatcggagc tcggccccca
      481 ccagcgccgc caccagccgc tggatcgcga gccctgccgg agagctgggc ttggacagca
      541 cgatggggga gccctcgttc acggaccgga tcatgtccac gtccgcgaag gggatctcgg
      601 ccgttggccg ggcccggagc aggctttcga catcgcgcgt cttcagcagt tcgcgggccg
      661 agatgtggtt gacgacgaaa tgggtcctac cctgaagaat cgccgcgtcg gccaggaact
      721 gcatcaggtc gctcatggcc cgcaacgcgc cgagctccgg gaagacgacc aggacgctct
      781 cctgggcctg ctcgatgacc caaatcgagc ggggatcgag gcgagtccca cagtcgacca
      841 cgacgaagtc gaacatcgtc cgcagggtgc cgatcacctg ctcgacctcc tccacgctga
      901 tcccagccgc gctatccggg ctggtcggcg aggcgagcac gctcaaacct gagtcgtgcg
      961 ctgtcaggta gccggccagg gcttccgggt cggtgcgggc aatgtcgtcc accgccaggc
     1021 gcgcgaagtc gagcttgggc gagaggttga ggtgggtcgc gacctggccg aactggagat
     1081 cgagatcgac caggacgacc cgtgagccga cgacctccgc gttcgcgccg ccgctggcca
     1141 ggagcgccgc gcagttgacc gccagcgcgg tcgtccccac accgcccttg ggcgagaaga
     1201 acgcgatcat ctgaccggcg cccggggagg aaggtccggg agcgaccgct ccggcgcgaa
     1261 tcaggagcgc gtgaacccgc gcctccagct cgcgacgggc gaagccgccc tcgatgacat
     1321 cgtcagcacc ggcctccaga aaggcgattc gggcctcgac gtccccggga ggagccacga
     1381 ccaggattgg caccccatcg tccgcccgcg ccgcccggag cgaaccgatg gcgcccgtgg
     1441 cggtcaaccc ctcggcaatg acgaggtcga tgtcgccggc gccgagagcc gcaacggcct
     1501 cggcgggggt tgccacttcc tggacgtcct gatccgaggt gctcaatgcg ctgcgcagcg
     1561 cgttccggag tgcctcatcc ggagcgcaga tcaagatgga cggccgaccc ggcacagaca
     1621 gcctcacgtg caagcagaaa agcgattcag gatagtcgtc gaccgcctgc cgcgccaacg
     1681 ccacgccggg cggcctcggc tatactcgct ccgcgcctcc cacccggagg cggttctgtc
     1741 ccacctttct gccgcaccgc ctcggtgagt gccgcaaacc cagagagagg agcgtccggc
     1801 ccgtgcgccg atacgaactc atgctgctgt tccggcccga cctggaggac gaccagctcc
     1861 agtccgcggt cgagcgcgtc acccgtgcca tcgtcaatgc cggcggaagc ctgtccaaga
     1921 tctcgccctg gggcaagcga cggctcgcgt accccatcca gcaccaccgt gacgcgtcgt
     1981 acttcctgat tcatttcgac atcgagccgg cggccgtgcg cgagatcgag cgcggcatcc
     2041 tcatcagcga ggagattctt cgccacctcg tggtcgtgct cgagccgcgt cagcggcgcg
     2101 acggcgaggg cgagatggac ggtgaaaccg aaggcgcggc ggtcggtgag atcgacggcc
     2161 tcgctccact gccggtctcc gacgacgagc cgacggctca ggcgctgccg gagccccagg
     2221 aaaccgacga accgcgcgag gcggctgcca cgcgcgaaga cgaacccgac gagacggacg
     2281 tcgtcgtcga ggatgtgaac gcaccggagg aggtcgcggg atgacgctga acaagatgat
     2341 gatcatcggc aacctggggg ccgatcccga actccgctac acgcccggcg ggaaggccgt
     2401 cacgaacctg cgggtcgcgg tcaacgaccg ccaccgcggt gcggacggcg agtgggtcga
     2461 ggagactcag tggttccgcg tggagctctg ggaccaggcg gccgagcgtg ccgccgagcg
     2521 cttccgcaag ggcaacaagg tctacgccga aggccagctg cgcgtgcgcg aatacgaagg
     2581 caacgacggg cagaagcgct cgtcggtcga gatccgcttc gcccgggtca tctctctcga
     2641 gcgacgcgat ccaggcgagg gcttcggcat cggcgacgcc gccagcgatg ccccgccggc
     2701 cgccgtgccg cggggcgccg cgcagaccgt cccgtcggat ggcccggcca ccgagacggt
     2761 cgacctcgac gacattccct tctaacgagg atccaaagca cgcatgccga gtccagctcg
     2821 cccccgccgc cgggaatcga cggccgacta ctaccgtgac cggcggcgcc ggaaggtctg
     2881 caacttctgc gtcgacaagg tcgatgcgat tgactacaag gaggtcaatc ggcttcggcg
     2941 ctatctttcc gagcgcgcga agatcgagcc gcggcggaag accgggacct gcgcccggca
     3001 ccagcggatg ctgaccgtgg ccctcaagcg cgcgcgccac gtggccctcc tgccgattgc
     3061 gccccagcac gtccgcccct gacgggctga cgggctgacc agcgcggtcc ggcggccacc
     3121 gctatggacg tcatcgtctt catcgctgcc ctggcgatca tcctgctcgg tgccgagctg
     3181 ttcacgaacg gcatcgagtg gttcggccat cggctgaacc tgggcgaagg ggcggtcggg
     3241 agcgtcctcg ccgcggtcgc gaccgccctg ccggagaccc tgatcccggt catcgcgatc
     3301 atcggtccgc tcttgaccgg gaacgaggcg accgccgggt cggaggaggt aggagtcggc
     3361 gcgatcctgg gcgccccgtt caccctggcc acgctcgcga tgttcatcac cggcctcgcg
     3421 gtcctgatct cggcccgaaa cggcggacgg tcgacggaca tgaaggtcaa cgtcaacgtg
     3481 atggcccagg acgtgatctt cttcctggtc agctacacca tcgcgatcgg ggcggcgttc
     3541 ctgcccgagg aatggcgctg ggccgacttc gcggtggtcg cggtcctgac cctggtctac
     3601 gtcttctacg tgcgggcgca cttccgcgag aagggcgagg gtgaggccgc cgatctcgcc
     3661 cagctccatg ccacccggct gccgggcatg tccggcggcg tcgcgctcca ggaggcagag
     3721 ggcgagggcg gggctgccct cgaggagggc ggcctgccac ggatgtggat catcgtgctc
     3781 cagaccatcg tcgccctggc ctgcatcatc ctgggcgccc ggatcttcgt gaccgccgtg
     3841 gagcacatct cggccggtat cggcttcgac ccccgcctgt tcgcactgat cgtggcgccg
     3901 atcgcgaccg agctgccgga gaagttcaac agtgtcctgt gggtccggaa ggggaaggac
     3961 accctggcca tgggcaacat caccggcgcc atggtcttcc aggcctgctt cccgaccgcg
     4021 atcggcatcc tgttcacctc ctgggcgttc accgccgaga acgcgctctc gttcgcgtcg
     4081 gccggtgcgg cgatcctggc gacgctcctc atcctggggc cgatgctccg cagccgacgc
     4141 ctgagcgccg ccgccctgct gattggcggc ccgatctacc tgatctacat cctggcggtg
     4201 atcttccttc cgtccggggg ttgacgaatg aggccgggcg tatacttccc gccggtgccg
     4261 agacctcgtc ccgcccacgc gttccggccg gtgaggagaa tgccttgttg aagcaggaga
     4321 cctcgactgg cgtgcagccc cttccccgtt ccacggctgg cctctgggca tatttccgcg
     4381 gcgagctcgt tccgctggat gacgcgcgca tctcggtcac gacccatgcc ttcaactacg
     4441 gcaccgcggt gttcgagggc gtccgcgcgt actggaatcc ggagcaggag cagctatacg
     4501 ggctggacct gctggctcac ttccagcgcc tccatcggtc ggctccgatc ctccagctcg
     4561 agctgcgtct gtctcccgag gagctggtcg agatcacctg cgagatgctc cggcgcagcg
     4621 agatgcgcga ggacgcctac atccgcccga ttctctacaa gtcgacggag gcggtcggcg
     4681 ttcggctcca caacctcgat accgatctcg tcatcttcac cctgcccttc ggcaagtaca
     4741 tcgataccga gggcggcatt cgcgcccagg tcagcagctg gcggcggaac gatgacaacg
     4801 cgatcccggc gcgggccaag atcaccggcg ggtacgtgaa cggggcgctc gccaagtcgg
     4861 acgcgcagct gaacggctac gacgaggcga tcatgctgac ccaggacggc cacgtcagcg
     4921 aggggagcgc ggagaacctg ttcatcgtcc gcgacggcgc gctgatcacc ccgtcgatca
     4981 cggacaacgt gctggaaggc attacccgcc gccacatcat ggaagtggcc ggcgacctgg
     5041 ggatcggagt cgtcgaacgg gtgatggatc ggaccgagct gtaccaggcc gatgaggtct
     5101 tcctgtgcgg cacgggcgcc cagctggccc cggtgatcga gatcgacaac cgccccatcg
     5161 gtggtggcac gcccggaccg atcacgcgac gcctgcacac gatctacttc gacgccgttc
     5221 ggggccggtc cgacacgtac cgcggctggc tgacgccggt ctactgatcg ccgtcatggc
     5281 ggtgaacggg gagcggcttc ggtgacggcc gcgacgggac cagtaatcgt ttccctggcc
     5341 cgcgacgccc gccatgccct cgcgctccct ggcctgctcg tgcttgtggg ggtcggcatc
     5401 gccgccgctg gtatcggtct cgggctggcg ctgtccatca tctcgctggt tgcgctggcc
     5461 gcggtggtcg gcattgcgct ggctgggatc ggcgcgtgga tggcgatccg cattcgctcc
     5521 ctgaagctgg cggtcgaggt cgactatctg cacctcaccg gccgcggcgt ggatcgtcga
     5581 tatcacctcg cccacgggtc gctatcgagg gtggcgacca gcgggccggc cggcgcacga
     5641 ctccggactc gaccgacgtc gctcggctgg ggcgtgggcc gaagcaccct gggcagcggc
     5701 gagcggatcg acgtcatccg gctcggcgcg acgccgtcgg tagtcctgat tcccaccgag
     5761 cgtggacgcg tggcgctggc ggccgccgtc gaggccgatc tcatccaggc gttgatgggt
     5821 gcggcccaga cccgtgccga gcgggagccc actccggcgg tggtggcggc ggcttcggtt
     5881 gccccggctc cagtggcagc cgccgcgata gttgcccctc cgattgcgcg gccacccgag
     5941 cccgaggcgc cgagacggcc gctgaccggc atcgagcgca tggcactcga ggagcagatg
     6001 gccgaggagc atggcgccgc gtacggcgga gcgccgcgcg agcaggcgat ggcgagcttc
     6061 tcgcccgcca cctacccgcc ggccacctac ccgccggcca cctacccgcc cgccacgccg
     6121 gtggtgcccg ccacgccacc gctggcggac gctccggtcg tcctccccac acccgtttcc
     6181 gccgccgcgc tgcctgcgat cgccgccacg accgcggctc cggtggtcgc gaccaccgtt
     6241 tcgcggacgg ccagcacgcg gtcgtggccg ctggccgtcc cacgcggcct gccgcgacgg
     6301 cgcccgctcc ggcccatggc ctggcgggcg agccggccaa ccctcgtccg cgagatcgcg
     6361 ctgctcggcg tccccctact catggcgggc gtcgtgtggt tcgtggccgc ccttgtcggg
     6421 gccaccggcg agggtcaggg actcgatccg ctgagcttcg ccctgctgct ctgcggcccg
     6481 gtcgcggcgc tcgccatctt cctggcgcgg acccgatggc cacggctcgc tggattgagc
     6541 tcactggccg cggtcttttg cctcgccctg gtggcccgcg ccgtcatcgg ctgaacccgg
     6601 caccggatct tgttgacaga ccgcgccggc ggctgccacg atcggggcaa tcgaacaccg
     6661 gccgcgacgg agcccatgcg gcgggtcatc ggtgccgacc agcgagcccg gatggtggaa
     6721 gcgggcaggt taccgacccg ctgatatcgc tcccgagccg aaggcgccaa gcgagccagc
     6781 ctggcacgtg ggtaacgcct tccgggcctc gccacccgtt atccgtggca catcgagtgg
     6841 agcgccgtcc gcggcgccca agcgcgggtg gtaccgcggg agctgatccc gtcccggcat
     6901 gggacgggat ctctgtctgt ctgggaggtg aacgagcacg tgtttcggcc agtgagtccg
     6961 ggtctggacg tgcccgccgt cgagcgggag gtcgccgagc gctggcgcgc ggagcgcacc
     7021 gccgagcggt acctggtccg gaacgcggag tccgatacga ggtacagctt cctcgacggc
     7081 ccgatgaccg ccaacaaccc gatgggcgtg catcacgcct ggggtcgcac ctacaaggac
     7141 ctttaccagc ggtaccacac catgctgggc gaccggcagc gctaccagaa cgggttcgac
     7201 tgccagggcc tgtggatcga ggtcgaggtc gagcgcgagc tggggttcac gaacaagcgc
     7261 gacatcgagg cgtacggcat cgacaagttt gtcgaggcct gcttcgcccg ggtccagcgc
     7321 tttgccgatc ggatcaccga gcaaagcaag cgcctgggct attggatgga ctgggacaac
     7381 tcgtacttca cgaactcgga tgagaacaac tacacgatct ggcggttcct tcagcgttgc
     7441 caggagcgcg gcctcctgta ccgcggtcac gacgtcatgc cgtggtgtcc gcgctgcgga
     7501 acgggtctgt cgaacatgga gatcgcgacc gagggctatc gtgagctcag ccatctctcg
     7561 ctcacgtttc ggctgccgat cacgagcccg ggccacgggg gcgagtcgct cctggtctgg
     7621 acgaccaccc cgtggacgct ctccagcaat gtcgcggccg ccgtccaccc ggatctcgtc
     7681 tatgacctgg tcgagagccc cggcggcgaa cgctggtggg tcagccgcgg ctcgcgggcg
     7741 cgcgtcgcgc ccgccgcccc ggtggtgggc gaggccctgg gtaaggacct gctcggtctg
     7801 acgtatgacg ggccgttcga cgagctgccg gttcaggccg gcgtgacgca cacggtcatc
     7861 gcgtgggacg aggtgaccga tgacgagggg accgggatcg tccacatcgc tcccggctgc
     7921 ggtgaggagg acttcgccct tgccaagcgc gacacgttgg cggtcctcga cccgatcgac
     7981 gagttcggcg tcttcaagga gggctacggg tggcagaccg gccgattcgc gggggccacg
     8041 cccgacgcgg ccgatgacct ggccccggcc gtcgccgccg aactggaggc gaaaggcctg
     8101 ctcgtcgcgc gcgagacgta ccgccaccac tacccggtct gctggcgctg caacacccag
     8161 ctcctgttcc ggctggtgga cgagtggttc atcgccatgg atccgctccg cgagcccatc
     8221 agcgcggtga cccgcgccgt tcagtgggtg cccgacggga tcggtctgga agagcgcgag
     8281 ctcgactggc tcaagaacat gcgcgactgg atgatcagca agaagcgcta ctacgggctc
     8341 gccctgccct tctgggtgtg cgatgcatgc gaggcctggg aggtcatcgg ctccaaggac
     8401 gagcttcggg agcgcgcggt ggccggctgg gaggcgttcg agggccatag cccgcatcgg
     8461 ccatgggtgg atgcggtcga gatcgcgtgc caggcatgcg gtggccggtc gcgccggatc
     8521 cgcgacgtgg gcaacccgtg gctcgacgcg gggatcgtgg cctactcgac cctgaagtgg
     8581 gacaccgatc ccgagtactg ggc
//
LOCUS       DASYGU010000172         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1571668, whole genome shotgun sequence.
ACCESSION   DASYGU010000172 DASYGU010000000
VERSION     DASYGU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571668"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="hppD"
                     /locus_tag="VGO32_04725"
     CDS             complement(<1..860)
                     /gene="hppD"
                     /locus_tag="VGO32_04725"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEV7200090.1"
                     /translation="MATTKPAPTAETTDFLPLKGIDHVEFWVGNARQAAHYFRTLWGF
                     TPVAYAGLETGIRDRSSFVLVQNDIRFVFTAPLTPDGEVAEHVRKHGDGVHDIAFSVD
                     DVASSWRETTSRGAVSALEPTETPGDKGVLRTSAIHTYGEVLHTFVDRSDYAGTYAPG
                     FHRIKDPAPAGAGQTLEMIDHCVGNVPLGDMNRFVDFYREVLGFSQLVHFDDNAIHTE
                     YSALMSKVMQNGDGRIKFPINEPAEGKKKSQIQEFLDYYLDAGTQHIAMLTTDIVGTV
                     RTLRGRGVEFL"
     gene            947..1528
                     /locus_tag="VGO32_04730"
     CDS             947..1528
                     /locus_tag="VGO32_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200091.1"
                     /translation="MRSSLRLTLLLFALLAVGACTSSDQPTAPESPSAAPTASAAAAG
                     NLPPGCEPIDIRTPSGARIVLDGAWIEVDTPVQPMTWWIRTQGDCVWGAGQVDDVPPD
                     RTISAVPDLVQSLAGHIGSDLVITGEILWLGPMPIAAPSIPSRYAPLRMLIDVDDTGR
                     ILLREDREPGVSGPRCPAPGGYCPAPLVLQPAD"
     gene            1556..1816
                     /locus_tag="VGO32_04735"
     CDS             1556..1816
                     /locus_tag="VGO32_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV7200092.1"
                     /translation="MIILAIVIIGLVAGWVANRVVGARRGFTPLELFIVGLAGSVVGG
                     TLFSVLLGEGFELRPGGFIGASIGAILLLAVYGPIRDRMAAR"
     gene            complement(1813..2547)
                     /locus_tag="VGO32_04740"
     CDS             complement(1813..2547)
                     /locus_tag="VGO32_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV7200093.1"
                     /translation="MPQIHLHAEPGDYAPFVLLPGDPGRATMIASRFDGGLEAARLVT
                     EHRGLLGYTGTINGRPVSVQTTGMGGPSIGVVVEELIRLGGRQLVRVGTTGALRGDIG
                     LGDLIVATSATPIDGTVATFMSGKPYAPASDFALTHALVHAAEKLAVPHHVGPIASID
                     VFSHYHPDPQFVRPWRDAGILCVEMEASVLFYLAATKGVQAACLGVAVDIDSGEENQE
                     HTYLSPEELAAAVERMIDVAFAAEVG"
     gene            2617..3408
                     /locus_tag="VGO32_04745"
                     /pseudo
     CDS             2617..3408
                     /locus_tag="VGO32_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
     gene            3555..3989
                     /locus_tag="VGO32_04750"
     CDS             3555..3989
                     /locus_tag="VGO32_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200094.1"
                     /translation="MRSPTGDRVDLTGTWAGTGYLAGDDEVAWLLQLGDCVYGTVTSA
                     ELNSESGSEIARRSNLAGRVAADFKVDLDVIILDQPPGAFSFAEYTTMAMLIEWEEDG
                     RIRLREDRERGVEAIRCRGVLCPGATTAPIPVIWYRLDAGGP"
     gene            complement(3986..>4736)
                     /locus_tag="VGO32_04755"
     CDS             complement(3986..>4736)
                     /locus_tag="VGO32_04755"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEV7200095.1"
                     /translation="HPVGHNAGLAQGGPGGVVSAARRGGHMGILRAEGDWQALVARAL
                     AAVERPIPVDPRFVPRRAVPDPALATEDARRFDRSRFPTARDAATLLLIYPAEAELVI
                     LLTVRHAQLASHPGEISLPGGAVEEGDASLEDAALREAQEEIGLDPASVRVVGRLDPV
                     WIPVSNFELVPIVAVADHRPGLTLGLDEVTEVIELPLDRLLVPDGVTEEEITVPGVVL
                     RTGVYFWGGHRIWGATARTLSMLGTALENLP"
BASE COUNT          718 a         1610 c         1637 g          771 t
ORIGIN      
        1 aggaactcca cgccacgacc acgcagggtt cggacggttc cgacgatgtc ggtggtcagc
       61 atcgcgatgt gctgggtccc ggcgtcgagg tagtagtcca ggaactcctg gatctggctc
      121 ttcttcttcc cttcggcggg ctcgttgatc gggaacttga tccgcccatc gccgttctgc
      181 atcaccttgc tcatgagcgc gctgtactcg gtgtgaatcg cgttgtcgtc gaaatggacg
      241 agctggctga atccgagcac ctcgcggtag aagtccacga accgattcat gtccccgagc
      301 ggcacgttgc cgacgcagtg gtcgatcatt tcgagcgtct ggccggcgcc ggccggcgcc
      361 ggatccttga tccgatggaa cccgggggcg taggtcccgg catagtcgga tcgatcgacg
      421 aaggtgtgga gcacctcgcc gtaggtgtgg atggcggacg tccgaagcac gcccttgtca
      481 ccgggcgtct cggtcggctc gagagcggac acggcgccgc gcgacgtggt ctcccgccag
      541 gatgacgcca cgtcgtccac cgagaacgcg atgtcgtgga cgccgtcgcc gtgcttgcgg
      601 acgtgctcgg cgacctcccc gtcgggggtg agcggggcgg tgaacacaaa tcggatgtcg
      661 ttctggacga gcacgaagct ggaccgatcc cggatgccgg tctccagccc ggcataggcg
      721 accggggtga agccccacag tgtccggaag tagtgcgcgg cctgcctcgc gttgcccacc
      781 cagaactcga cgtggtcgat gcccttgagc ggcaggaagt cggtggtctc ggcggttggt
      841 gccggcttgg tcgtggccat gggtccgggt gcctcctgtt cgggaccgat ggtacgccgt
      901 ccagccaagc attccgcctg cgagccatcc cgtatgctcg gccgccatgc gatcgtcatt
      961 gcggctgact ttgctgctgt tcgccctgct cgccgttggt gcatgcacgt cctccgatca
     1021 gccgacggcc ccggagagcc catcggcggc cccgacggcc agcgccgctg cggccgggaa
     1081 cctgcctcct ggctgtgagc cgatcgacat tcggactccc agtggagcac gcatcgtgct
     1141 ggacggagcc tggatcgagg tcgacacccc tgtgcaaccc atgacctggt ggattcgtac
     1201 ccagggggac tgcgtctggg gcgcgggtca ggtcgatgac gtcccgccag acaggaccat
     1261 cagtgcggtg ccggacctcg ttcagagtct cgccggtcac atcgggtcgg acctcgtgat
     1321 tacgggggag atcctctggc ttgggccaat gcccatcgcg gcgccgagca tcccctccag
     1381 gtacgccccc ctgcggatgc tcatcgacgt cgacgacacg ggccggatcc tcctgcgtga
     1441 ggaccgtgag ccgggagtgt ccggcccgcg atgtccggca ccgggcggct actgcccggc
     1501 gccgctcgtt ctgcagccgg ctgactaaac ggcgcgggcc tgtatcctgc gggccatgat
     1561 cattctcgcc atcgtcatta tcggtctcgt cgccggctgg gttgccaacc gcgtggtcgg
     1621 cgcccgccgc gggttcaccc cgctcgagct gttcatcgtc ggtctcgccg gctcggtggt
     1681 cggcggcacc ctgttcagcg tcctgctcgg ggagggattc gagcttcgcc cgggcgggtt
     1741 catcggtgcc tcgatcggcg ccatcctcct cctcgccgtc tacgggccga tccgcgaccg
     1801 gatggcggcc cgctagccga cctcggccgc gaacgcgacg tcgatcatcc gctcgacggc
     1861 cgccgccagc tcctcgggac tgaggtaggt gtgctcctgg ttctcctcgc ctgagtcgat
     1921 gtcgaccgct acccccaggc acgcggcctg cacccccttg gtcgcggcca ggtagaacag
     1981 gacgctggcc tccatctcga cgcacaggat cccggcgtcg cgccagggtc ggacgaactg
     2041 cggatcagga tggtaatgcg agaagacgtc gatgctcgcg atcggaccaa catggtgcgg
     2101 gacagccagc ttctcggccg catggacgag ggcatgggtg agcgcgaagt ccgaggccgg
     2161 ggcatacggc ttcccgctca tgaacgtggc gaccgtgccg tcgattggcg tggccgaggt
     2221 cgcgacgatg agatctccca ggccgatgtc accgcggagc gcaccggtcg tgcccacccg
     2281 aacgagctgt cgaccgccga gccggatgag ctcctcgacg acgacgccga tggatggccc
     2341 gcccatgccc gtggtctgga cgctgaccgg gcgaccgttg atcgtcccgg tgtagccaag
     2401 cagcccgcga tgctcggtga ccagccgggc ggcttcgagc ccgccgtcga accgtgaggc
     2461 gatcatcgtc gcccggcccg gatcgccggg aaggagcacg aatggcgcgt agtcgccggg
     2521 ctcggcgtgg aggtggatct gcggcatgaa ccaagcctag ccggtcaggg caacggctag
     2581 catgctggag ggagtcggta cgccgaggag ctcgagttcg cggtccggat ggcgaggtcg
     2641 gcagggacgc tgatcctcga gtcctacggc cgggttggcc gggttcggca caagagccgg
     2701 cgggacgtgg tgaccgatat cgactaccgc tccgaggcgc tcatcgccag gcgcatccgc
     2761 cgtcacttcc cggaggacgg gatcctggcc gaggaggccg gtgccgatcg ggtcggcggt
     2821 cccgatgggg cgcgacggtg gtgggcgatc gacccgctcg acgggacggt gaactacgcc
     2881 aacgggatcc cgtatttctg cgtctcgatc gcgctcatcg aggaccaggt gccggttctc
     2941 ggcgtcgtct tcgatccgct gcgcggcgac ctgtacaccg cgacgagcca gtcggccgcc
     3001 cggctgaacg gggcgctggc gaaggcctcg accaaggagt cgctcggcga ctgcgtcgtg
     3061 agcctggcga tcatcggccg aggtggtctg tcccgagaac ggaagatcgc gcccgagatc
     3121 cgcatccacc gtcggatggg gagcgccgcc ctgtccctcg catcggtcgg tgccgcgcgg
     3181 ttcgacgcat tcgtccagaa cggcgggctc agcctctggg acgtggccgc tgccgggctc
     3241 atcgcggaac gcggtggcgc ggtcgtcacc aacctggccg gcgggccgtg gtggcagcca
     3301 cggcgcaagg gcgcggtcca gagcatcgtc gccgctcccg agccgcacca cgccgagatc
     3361 ctccgcctgc tccgggaggc cgggacgcgg gtccacttgc gccggtgacc attcgttccg
     3421 ctgcgtttgc gctcatcgca atgctggcgg cgtgcgcgtc gccatccacg tcgccgtcgg
     3481 ccacctcatc cgcggctggc ccatctacga ccgaagtatt cgcgggcctg ccgactggat
     3541 gccacccgat tgacatgcgt tctccgaccg gcgaccgcgt cgacctgacc ggaacctggg
     3601 caggcaccgg atacctggcc ggcgatgacg aagtggcctg gctgctccaa cttggggact
     3661 gcgtatacgg caccgtgacc agcgcagaac tcaattcgga gtcaggatcg gagatcgctc
     3721 gtcgctcgaa cctcgccgga cgcgtcgccg cggatttcaa ggtggatctc gacgtcatca
     3781 tcctcgacca gccgcccggg gcattcagct tcgccgagta caccacgatg gccatgctga
     3841 tcgaatggga agaggacggt cgaattcgcc ttcgcgagga ccgcgagagg ggagttgagg
     3901 ccatccgatg ccgaggcgtg ctctgcccag gggccacgac cgcaccgatc ccggtcatct
     3961 ggtatcgcct ggacgccggc gggccctagg ggagattctc gagcgcggtt ccgagcatgg
     4021 acagcgtgcg ggcggtcgcg ccccagatgc ggtggccgcc ccagaagtag acccccgtcc
     4081 taagcacgac cccggggacg gtgatctcct cctcggtcac cccgtccggg accagcagac
     4141 ggtcgagcgg tagctcgatg acctcggtga cctcatccag gcccagggtg aggccgggcc
     4201 gatggtcggc caccgcgaca atcggcacca gctcgaagtt cgacacgggg atccagaccg
     4261 ggtcgagccg accaacgacc cgaaccgacg ccgggtccaa cccgatctcc tcctgcgctt
     4321 cgcggagcgc cgcgtcctcc aggctcgcgt cgccctcttc cacggctcca cccgggagac
     4381 tgatttctcc cggatgggag gccagttggg catgccgcac ggtgagcagg atgaccagct
     4441 ccgcctcggc gggatagatg aggagcaggg tggcggcatc ccgggcggtc ggaaaccggg
     4501 atcgatcgaa gcgccgcgcg tcctctgtgg cgagcgcggg gtccggcacg gcacggcgag
     4561 gcacgaaccg cgggtccacc gggatgggcc gctcgacggc cgccagcgca cgagccacca
     4621 gggcctgcca gtcgccttca gcccgtaaga tgcccatatg acctccgcgc cgcgcagccg
     4681 agacgacgcc acccgggccg ccctgcgcga gacccgcatt gtggccaacc gggtga
//
LOCUS       DASYGU010000173         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1160226, whole genome shotgun sequence.
ACCESSION   DASYGU010000173 DASYGU010000000
VERSION     DASYGU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160226"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..744)
                     /gene="tmk"
                     /locus_tag="VGO32_04760"
     CDS             complement(91..744)
                     /gene="tmk"
                     /locus_tag="VGO32_04760"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV7200096.1"
                     /translation="MALTEPSASRGRFITLEGPEGSGKSTAARHLADWLVTRGVRATV
                     TAEPGGTRLGEEIRRLVLHARSISDDLDPRADALLFAAGRAQHVARRIRPALDSGEWV
                     ICARYLDSSLAYQGGGYGLDLDTLRTLQAFATGGLKPDLTILLDVPVDIGLRRKRKDR
                     WNRFEDTESAAFFERVRGTYLQLAAAEPDRFRVLDGSGSVDETDLAIRAVVAPLLDR"
     gene            complement(735..2459)
                     /locus_tag="VGO32_04765"
     CDS             complement(735..2459)
                     /locus_tag="VGO32_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEV7200097.1"
                     /translation="MPSLETTDDLDALLESLPPEVVERVRALGARADLLEIVLDLGRR
                     PEARFTHGEEVLLEREVTEADIGFVVDHIGAFGDDNRAGIERTLHRISAIRNRAGKVV
                     GLTCRIGRAVFGTIDIIRDIAESGQSILILGRPGVGKTTMLREVARVLADDLGKRVVV
                     VDTSNEIAGDGDIPHPGIGDARRMQVRTPTEQHAVMIEAVENHMPEVIVIDEIGTELE
                     AGAARTIAERGVQLVGTAHGNTLDNLMLNPTLSDLIGGIQTVTLGDEEARRRGTQKSV
                     LERKAPPTFDVVVEIVERDRVIVHRSVAETVDAILRGHMVPPESRWRDGAGEVRAATK
                     YDYRIHESAAGSMFVTPETGGHGTFRRSGASGLHPLPGGRGRGTVESPDARRDIEEAM
                     LASDRSPTSDASSGEPRSTREARVASLRPMALFPYGVSRKRLEQAVRELSVPVTVARD
                     LEDADAVVTLRTYYRRKPPALRDAEAGGTPLFVLKTNTLVQMESMLANLYELEADPQE
                     AALRETAEAIGLVRDSGNSVELAPQNAYVRRLQHQMAEQHNLMSRSRGSEPNRRVQLL
                     PDEGRAWR"
     gene            complement(2483..3541)
                     /locus_tag="VGO32_04770"
     CDS             complement(2483..3541)
                     /locus_tag="VGO32_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200098.1"
                     /translation="MTTVANEAGEVRAARLTDRQALADLSGQVHAAPDAYRRSLGVPA
                     PTGSPGIELSTLIPSWLPLRSPSVHLVAEADGQLIGSCRAITEPHGDDWVIIELDAAD
                     GPMAGEVRWAMLEALIEEGAKRNVARYHVACADVHENIQLFGMAGFMAYAQEEILYRA
                     AETPGRRGGGFLRRRRRSAARRGSAPTDIPLQPAGAPDAWHLFDLWSHCTPPAIARVE
                     DYHAADWESVGHEAVVPRSSLNPLLHFGEVTAWLFTVDQRAGGFAQHGASRVGPHYLR
                     FLVRDGTDGEALLASVLGRLSAEAMAAGILAPVRTYEAAGLSAAAGIGFVPVGRVTLL
                     VREVRALVRQPALVPAVN"
     gene            complement(3538..4143)
                     /gene="pdxT"
                     /locus_tag="VGO32_04775"
     CDS             complement(3538..4143)
                     /gene="pdxT"
                     /locus_tag="VGO32_04775"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV7200099.1"
                     /translation="MVDTARRPRVGILAVQGDVAEHATALREIGAEPVQVRLPRDLAG
                     VEALILPGGESTAMRRMIDAYGLAEPILSLAASGAPMWGTCAGMILLARRIADGDPPT
                     LPLLDIEVRRNAYGRQLDSFEADLDVPSLGDEPIHAVFIRAPVVTDVGPGVEVLATDP
                     MGRTVAVREGRLLATAFHPELTGDRRFHRALLDLIGVEAPA"
     gene            complement(4137..5039)
                     /gene="pdxS"
                     /locus_tag="VGO32_04780"
     CDS             complement(4137..5039)
                     /gene="pdxS"
                     /locus_tag="VGO32_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV7200100.1"
                     /translation="MTTTERPPQTSTWATKVGLARMLAGGVIMDVVTADHARIAEEAG
                     AVAVMALERVPSDIRKDGGVARMSDPKLIEEIKQAVTIPVMAKSRIGHFVEAQILQSL
                     EVDYIDESEVLTPADEAHHIDKHRFAVPFVCGCRDLGEALRRIGEGAAMIRTKGEAGT
                     GNIVEAVRHMRAVRSGIAQLRAMREDELFAAAKELRAPLEVVRQVAELGELPVPNFSA
                     GGVATPADAALMRQLGAEAVFVGSGIFKAEHPEQVARAVVKATTHYDNPEILAEVSAG
                     LGEPMRGLELTEIPDQERLASRGW"
     gene            5090..>5502
                     /locus_tag="VGO32_04785"
     CDS             5090..>5502
                     /locus_tag="VGO32_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7200101.1"
                     /translation="MINAPVLVLNQNYEALNVCDIRRAFRLLGAEKAELIARNHQVIH
                     TPTRAYEAPSVIRLVYLVKRPQPRVKLSRREVFARDRHTCQYCGRQAHDMTLDHVVPR
                     HRGGKHEWENLVAACRRCNHRKGSKTLAEAHMYLGR"
BASE COUNT          768 a         1976 c         1949 g          809 t
ORIGIN      
        1 gtattccttc tcgaggaggg catccaccag ggctccggcg tcctcggcat ggacgatggc
       61 ggtgaccagc ttcatcggtt tcagtatgcc ctaccgatcc aggagcggag ccaccactgc
      121 gcggatggcg aggtcggttt catccaccga accggacccg tccagcaccc gaaaccgatc
      181 gggctcggcg gcggccagct gaagatacgt tccgcggacc cgttcgaaga acgcggcact
      241 ctcggtgtcc tcgaatcggt tccatcggtc cttccgcttg cggcgcagac cgatgtcgac
      301 cggcacgtcg agcaggatcg tgaggtcggg cttcaacccg ccggtggcga acgcctggag
      361 cgtccgaagc gtgtccaggt cgagcccgta gccgccgccc tggtacgcga gcgacgagtc
      421 gagataccgg gcgcagatca cccactctcc gctgtccagc gccgggcgga tgcgacgggc
      481 aacatgctgc gcacggccgg ccgcgaacag gagcgcgtca gcccgggggt ccaggtcgtc
      541 cgagatcgat cgagcgtgga gcacgagtcg ccggatctct tcgcccagtc gggtcccgcc
      601 gggctcggcg gtgaccgtcg cccgcacgcc gcgggtcacg agccagtccg cgagatggcg
      661 ggcggccgtg ctcttcccac tgccctccgg cccttcgagg gtgatgaacc ggccgcgact
      721 ggccgacggc tcggtcagcg ccaggcgcgc ccctcgtcgg gaaggagctg aacccggcgg
      781 ttcggctcgc tgccccgcga gcggctcatg aggttgtgct gctcggccat ctggtgttgg
      841 aggcgacgca cgtacgcatt ctgaggggca agctcgaccg agttgccgct gtcccggacg
      901 aggccgatgg cctccgccgt ctcacggagc gccgcctcct gcggatcggc ctcgagctcg
      961 tacaggttgg cgagcatcga ctccatctgg accagggtgt tcgtcttgag cacgaacagc
     1021 ggcgtgccgc cggcctcggc gtcacgcagc gcgggcggct tgcgccggta gtacgtccgc
     1081 agggtgacga ccgcgtcggc gtcctcgagg tcgcgggcga ccgtgaccgg gacggacagc
     1141 tcgcggacgg cctgctcgag gcgcttgcgg ctcacgccgt acgggaacag ggccatcggc
     1201 cgcaggctgg cgacgcgcgc ctcgcgggtg ctgcgcggct ctccgctgct cgcatcgctg
     1261 gtcggggatc ggtcagaggc gagcatcgcc tcctcgatgt cccgccgggc gtccggtgac
     1321 tcgacggtgc ctcggcctcg gccgccgggc agggggtgga ggccggacgc cccggaccgg
     1381 cggaacgtcc cgtgcccacc ggtctcgggc gtgacgaaca tggatccagc cgctgactcg
     1441 tggatccggt agtcgtattt cgtggccgcc cgcacctcgc cggccccgtc ccgccagcgc
     1501 gactcgggcg ggaccatgtg gccgcgcagg atggcatcca ccgtctcggc cacgctccga
     1561 tggacgatga cccggtcccg ctccacgatc tcgaccacca cgtcgaaggt gggcggcgcc
     1621 ttgcgctcga gaacgctctt ctgggtgccg cgccgacgcg cctcctcgtc gcccagggtg
     1681 acggtctgga tgccgccgat caggtccgac agggtcgggt tcagcatgag gttgtcgagc
     1741 gtgttgccgt gcgccgtgcc caccagctgc acgccccgct cggcgatggt ccgcgccgcg
     1801 ccggcctcca gctcggtccc gatctcgtcg atgacgatga cctcgggcat gtggttctcg
     1861 accgcctcga tcatgaccgc gtgctgctcg gtcggagtcc ggacctgcat ccgccgggcg
     1921 tcgccgatgc ccgggtgcgg gatgtcgccg tcgcccgcga tctcgttcga ggtgtcgacc
     1981 accaccactc gcttgcccag gtcgtcggcc agcacccggg cgacctcgcg gagcatggtc
     2041 gtcttgccca ccccgggacg accgaggatg aggatggact gccccgactc ggcgatgtcc
     2101 cggatgatgt cgatcgtccc gaacaccgcc cgcccgatcc ggcaggtgag gcccacgacc
     2161 ttcccggccc ggttccggat ggcgctgatc cgatggagcg tccgctcgat gcccgcccgg
     2221 ttgtcatcgc cgaaggcgcc gatgtggtcg accacgaagc cgatgtcggc ctcggtcacc
     2281 tcccgttcca ggagaacctc ctcgccatgc gtgaagcggg cctccggccg gcggccgagg
     2341 tcgagcacga tctcgagcag gtccgcccgc gcgccgaggg cgcggacgcg ctccacgacc
     2401 tcggggggca gcgactcgag cagcgcgtcg aggtcgtccg tcgtttcgag gctcggcatt
     2461 cgctcctcct ggtcgcgcac gctcagttga cggccggcac gagcgccggc tgacggacca
     2521 gcgcccggac ctcccgaacg aggagggtca ctcgcccaac cggcacgaag ccgatgccgg
     2581 ccgccgcgct gaggcccgcc gcctcgtatg ttcgcaccgg cgccaggatg ccggcggcca
     2641 ttgcctccgc ggacagccgg ccgaggacgc tggccagcag cgcctccccg tcggttccgt
     2701 cgcggacgag gaagcggagg tagtgcggcc cgacccggct ggccccgtgt tgcgcgaagc
     2761 cgccggcccg ctggtcgacg gtgaacagcc aggcggtcac ctcgccaaag tggagcagcg
     2821 ggttcaggct cgatcggggg acgacggcct cgtggccgac cgactcccag tcggcggcgt
     2881 ggtagtcctc gacccgggcg atggccggtg gcgtgcaatg gctccacagg tcgaagaggt
     2941 gccacgcgtc gggcgcgccg gccggctgga gcggaatgtc ggtgggagcc gagccccggc
     3001 gagcggccga ccggcgtcga cggcggagga acccgcctcc gcgccgcccc ggcgtctcgg
     3061 ccgcgcggta caggatctcc tcctgggcgt aggccatgaa gccggccatc ccgaacagct
     3121 ggatgttctc gtggacgtcc gcacaggcca cgtggtaccg ggccacgttg cgtttggccc
     3181 cctcctcgat gagcgcctcg agcatggccc agcggacctc gcccgccatc ggtccgtccg
     3241 ccgcgtcgag ctcgatgatg acccagtcat cgccgtgcgg ttcggtgatg gcccggcagg
     3301 agccgatgag ctggccgtcg gcctcggcca ccaggtggac cgacggcgac cggagcggca
     3361 gccaggaggg gatcaaggtg gacagctcga tgcccgggct gccggtcggg gcggggactc
     3421 ccaggctgcg ccgataggca tcgggcgccg cgtggacctg gcccgagagg tcggcgagag
     3481 cctgtcgatc ggtcagccgc gccgcccgaa cctccccggc ctcgttcgcc acggtggtca
     3541 tgccggcgcc tcaacgccga tgagatccag cagcgccctg tggaagcggc ggtcgccggt
     3601 gagctcgggg tggaacgcgg tggccagcag gcgcccctcg cgcaccgcga ccgtgcggcc
     3661 catcggatcg gtggccagga cttcgacccc gggcccgaca tcggtcacga ccggggcgcg
     3721 gatgaagacg gcgtggatcg gttcgtcgcc cagggacggg acgtccaggt cggcctcgaa
     3781 ggagtccagc tgccggccat aggcgttgcg ccggacctcg atgtcgagca ggggcagggt
     3841 cggcgggtca ccgtccgcga tccggcgggc gagcaggatc atgcccgcgc acgtccccca
     3901 catcggcgcg cccgaggcgg cgagactcag gatcggctcg gccagtccgt acgcgtcgat
     3961 catgcgtcgc atggcggtcg actccccgcc gggcaggatc agggcctcga ccccggccag
     4021 gtcacgggga aggcggacct ggacgggctc ggcgccgatc tcgcgcaggg ccgtggcgtg
     4081 ctcggccacg tccccctgaa cggccaggat gccgacccgc ggccggcgcg cggtatctac
     4141 caaccccggg aggccagccg ctcctggtcg gggatctcgg tcagctcgag gccgcgcatc
     4201 ggctcaccca ggcccgcact gacctcggcc aggatctcgg ggttgtcgta atgcgtcgtg
     4261 gccttgacca cggccctggc gacctgctcc gggtgctcgg ccttgaagat gccgctgccc
     4321 acgaacacgg cctccgcgcc gagctggcgc atgagcgcgg cgtcggctgg agtggcgacc
     4381 ccgccggcgc tgaagttggg gaccggaagc tcaccgagct cggccacctg gcgcacgacc
     4441 tcgagcggcg cgcggagctc cttggccgcg gcgaacaatt cgtcctcgcg catggcccgc
     4501 agctgggcga tgcccgatcg cacggcgcgc atgtggcgga cggcctcgac gatgttgccc
     4561 gtgcccgcct cgcccttggt ccggatcatg gccgccccct cgccgatccg gcgcagcgcc
     4621 tcgcccaggt cgcggcagcc gcacacgaac gggaccgcga accggtgctt gtcgatgtgg
     4681 tgcgcctcgt cggccggggt caggacctcc gactcgtcga tgtagtccac ctcgagcgac
     4741 tggaggatct gggcctccac gaagtggccg atccggctct tggccatgac cgggatggtg
     4801 accgcctgct tgatctcctc gatcagcttg ggatcgctca tccgggccac gccgccgtcc
     4861 ttgcggatgt cggacgggac ccgctcgagg gccatcaccg ccacggctcc cgcctcctcg
     4921 gcgatgcggg cgtggtcggc ggtaacgacg tccatgatca ccccgccggc gagcatgcgg
     4981 gcaagcccga ccttggtcgc ccaggtcgag gtctgcgggg gtcgctcggt ggtcgtcatg
     5041 aggcccgcat tgtacgctcg gctatgatcg cggccactgg aggcggagat tgatcaacgc
     5101 accggtcctg gtcctcaatc agaactacga agccctcaac gtctgcgaca tcaggcgggc
     5161 ctttcggctg ctcggggccg agaaggcgga gctgatcgcc cgcaaccacc aggtcatcca
     5221 cacccccacc cgcgcatacg aggcaccctc ggtgatccgt ttggtgtacc tcgtcaagcg
     5281 accgcagccc cgggtcaagc tcagccggcg cgaggtcttc gcccgcgacc ggcacacctg
     5341 ccagtactgc ggccggcagg cccacgacat gaccctcgac catgtcgtgc cgcgtcaccg
     5401 tggcggcaag cacgaatggg agaacctcgt ggcggcctgc cgccggtgca accataggaa
     5461 ggggtcgaag accctggccg aggcccacat gtatctgggt cg
//
LOCUS       DASYGU010000174        14214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_902691, whole genome shotgun sequence.
ACCESSION   DASYGU010000174 DASYGU010000000
VERSION     DASYGU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902691"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1670
                     /locus_tag="VGO32_04790"
     CDS             63..1670
                     /locus_tag="VGO32_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV7200102.1"
                     /translation="MSGNERRPRFTTQSGVEINRLYTPEDLAGWDAARDLGEPGGPPF
                     TRGIHATGHRGRLWTMRMFAGFGAAEDTNARFRRLIAEGGTGLSIAYDMPTLYGYDHD
                     EPEAAGEFGTCGVAVSSLADMEILLSGLPIGEISTSMTINSPAAMIWAMYIVAAEKAG
                     VPRAELTGTLQNDILKEFIAQKEYIFPPAPSLRLVTDTVEFGTRELPRWNTISISGYH
                     IREAGSTAVQELAFTIADGIAYVEDAVARGMRFDDFAPRLSFFFNSHNDFFEEIAKFR
                     AARRIWYRLCTERFGAENERSTWMRFHTQTAGVSLTAQQPQNNLTRVAIQALAAILGG
                     TQSLHTDAYDEAWAVPSEAAALLALRQQQILAEESGVANTVDPLGGSYFVEALTNKTE
                     AAAWDYIRRIDDMGGMLRAVEAGYPQAEVADAAYAQSRALESGEHQIVGVTRYADPNE
                     ELAIPLLEITEEMERRHLDRLTRVRAERDADAHARAITRLREESARDGVNLMPAIVAA
                     VEAYATIGEMCGVLREVFGEYREPLAV"
     gene            1704..2576
                     /locus_tag="VGO32_04795"
     CDS             1704..2576
                     /locus_tag="VGO32_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEV7200103.1"
                     /translation="MQVHLVDATFELFRAYHSRPAETGPDGRSVNAVRGLVDSLLSLL
                     REPDVTHVGAATDHVIESWRNDVYPGYKSSAGLPWDLLSQFELAEDAIRALGITCWAM
                     VEDEADDALATVTARVAADPRVEQVVICTVDKDLAQCVSGERIVQRDRMRGITYDEAG
                     VIAKFGVPPASITDYLALVGDSADGYPGLPGWGSKSAASVLARWEHLEAIPDDPLTWD
                     VDVRGAGRLAVALRQGRDEAYLYRRLATLNRDSDAPGDVADLEWHGVPRAPFETLCAA
                     LGFESIPGRVHRWA"
     gene            2661..3803
                     /locus_tag="VGO32_04800"
     CDS             2661..3803
                     /locus_tag="VGO32_04800"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEV7200104.1"
                     /translation="MPRRIAAASVLAAVALFGSGLPGAVVPASASSCSGWTSETLPPP
                     TIRVYRAATGAVETVDFRVYAKNVLSREWIGSWTTESLRSGAVAVKMYAWYHVLHWRG
                     LTNATGQCFDVYDDTRDQVYNPAQPTWPSAASAVDATWATRALKNGAISPTYYNAGSA
                     NEACGANANGWRMYQWGTQACGLAGRTAAQILLTYFYPGWTVTGAPPPPPSPTPLPTP
                     VPTPAPTPAPTPGPSVPAPTPAPPPAPTPAPTPAPPPPPPSGGNPGGGQSGIVGQSAP
                     PPPPSSQPEPVMVAPAPAAADEPPADPAPWLRRPAASDRLFEGTERVSTKHLAPAAPP
                     PAWSYRAMAPSAIAEVAVQLAIPLFIGRPDPALNAPPVDLPAVVGR"
     gene            complement(3822..4781)
                     /locus_tag="VGO32_04805"
     CDS             complement(3822..4781)
                     /locus_tag="VGO32_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEV7200105.1"
                     /translation="MELIDAATLRALVPMDRLLDAIEAAYRDVAAGRDRSPLRQHLEL
                     TGGGLLVLMPAVREGGSGAAVKLITWVPGNPERGLPTIQGTVTWFDAVTGEAAFQFDG
                     PAVTALRTGAASGVGARLLARPDASVMALIGAGVQAEPQIRAVLAARPIREVRVYART
                     REPREAFAAAMDVALPTSVRAADSAEEAVRGADIVCCVTPSETPVFEAEWLEPGTHVN
                     AIGAFRLGMVEIPADAFGRASLVAVDSREAVGHEAGDVAAAIAAGMISEADLVEIGTV
                     AADWAECRDPAAITIFKSVGLPIQDLATADLAVAAWRAGGGRG"
     gene            4998..6230
                     /locus_tag="VGO32_04810"
     CDS             4998..6230
                     /locus_tag="VGO32_04810"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7200106.1"
                     /translation="MPTPAAPVLRLRDTRRAAIHPVEPLGAGPIGLYVCGITPYDTTH
                     LGHAFTYLAFDVIHRYLEYAGHDVRYVQNLTDVDDDMLARARDVKEDYLALGTRHADR
                     FLADMAALNWLPPDVYARATEHVPQVVAMIERLEAAGLAYRGDGHVYLSTIASPDWGS
                     LSHVSPPDQLALANERGNRPDLPGKRDPLDPVLWQRSLADEPAWPSPWGSGRPGWHIE
                     CSAMSVTHLGPRFEIHGGGEDLAFPHHEAERIQSEGATGERPVVGHWVHTGMVRYDAN
                     KMSKSLGNLVMVADLLDRWPADAVRLALIRHSYRAELAWTDGLVVDAAEVAARWTTAA
                     ARAGAVEPDSLPDEVAAGRAVVLDSLADDLDTPGAIAALDVLAGLALEPADVAAAAGW
                     VLRDLATRILGLRLGGTR"
     gene            6227..6736
                     /locus_tag="VGO32_04815"
     CDS             6227..6736
                     /locus_tag="VGO32_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200107.1"
                     /translation="MIDDNRPPSVQLGEVVPPEDPEDWGQPLTWIVAAGMLVTPLAGA
                     AWFILAAPTDPRLSVEGISALAVILALGAAITGATQRGMWRTFVTSIGAGLFNALGLV
                     VVGTVLAGGASLGVATVAAVAGVIATVPTAMLAALLADSAGRGRRIVSPALAGGVTAL
                     VIVRLMTSL"
     gene            complement(6708..7736)
                     /locus_tag="VGO32_04820"
     CDS             complement(6708..7736)
                     /locus_tag="VGO32_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200108.1"
                     /translation="MTEPLVVADDLHVRFATQRPLTDSLLRRPAPSVRAVDGVSFAVS
                     PGEVLALVGESGCGKTTTGNLLLGLIPLSEGTVRFEGQDVASMGRRQMRAFRRACQII
                     FQDPYESLNPRMRIRDIIAEPLRVHHVAVNDDDEQTRVRHALTQAGLAPPEVYENRYP
                     YELSGGQRQRVVIAAALTLEPRLLIADEPVSMLDVSIRADILNLLRRLATESRIAIVM
                     ITHDLATVSAYADRLAVMYLGRIVEIGDTRTILSDPQHPYTRALLSVVPTTDPAARQR
                     PQVLAGETPDPSRIPSGCRFHPRCPVAVAACRTIDPALIALGPDHEAACILVGSGPIV
                     RGSSSAGR"
     gene            complement(7729..9693)
                     /locus_tag="VGO32_04825"
     CDS             complement(7729..9693)
                     /locus_tag="VGO32_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEV7200109.1"
                     /translation="MTVVAEVQSRPTTIAAPRGALRTLLHHPVGLAGLIMLGLTFFVA
                     VFAPLLAPYDPYDTPPVHIEDIYQPPSPEHPLGTDDGGKDVLSELIYGSRVSLIVGFT
                     AAAIAIVIGGAIGIVAGYKAGRTGSALMRFTDFFLVIPDLALQIVLVAILGPSLVTII
                     IVIGILGWTTTARLVRSQALTVRERKFVARAHAIGASDWHMIRRHIIPLVMPLMLANA
                     VLVISLAILSESTLAFIGLGDPTVISWGKMLNFAFDRGAVSAGAWWAFFPPGLAIVWV
                     VLATTLLGTALEDVLNPRLKRHHLEAGRDDVVRSVEPPPAVMIPTANAPLLSIRGLSV
                     EFESSDGPLRAVDDVSFDLARGETIGLVGESGSGKTTAMLGLLRLLPPGGRIVAGQAL
                     FDGEDLLSLPPDELRAFRWTRLSMVFQGAMNALNPVQTVGGQVTEAIRLHDHRISKDE
                     ANRRTADLLDRVGIGARRANEYPHTYSGGMRQRAMIALALACRPDVIVADEPTTALDV
                     MIQAQIMELLGGLSRDLGMSTIIVTHDLGIVAQVCDRVVVMYGGRVAEDAPASRLFAE
                     PRHPYTQALLEAFPDVAHPERELHAIGGTPPRLDAMPPGCPFAPRCAHAFDRCDTAKP
                     PLYPISDGWAASCYLLDPAHPAPASPATHD"
     gene            complement(9697..10704)
                     /locus_tag="VGO32_04830"
     CDS             complement(9697..10704)
                     /locus_tag="VGO32_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200110.1"
                     /translation="MSGRTRFLARRVGWALVTVWVVITFNFVLFRVLPGDPARSGARD
                     PRLNPETQAALTERFGLDKPLFFNFGSGAPFDSQYFIYIGALMQGDLGTSYARPDDPV
                     AEQLGDALVNTLWLVLPSQFLSILFGIILGLVAAWRRGKATDIGSLAFSLFMWSLPTF
                     FLAIILLFIGARYFDLPIGGKGTIGAAFETPVEAAVDLFRHLLLPTLTLTLVLLGEYM
                     LIMRSSVLDVFSEDYVLTAKAKGLSTYRIIRGHALKNAMLPMVTLIALNLAFTVSGAI
                     QVESVYSWPGLGHLTVQAVSDRDYPVLQGAFLLLALAVVFANLVADIVYGYLDPRVTE
                     A"
     gene            complement(10763..12463)
                     /locus_tag="VGO32_04835"
     CDS             complement(10763..12463)
                     /locus_tag="VGO32_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7200111.1"
                     /translation="MRRPLRPAAALIGLTMVLASCQGSASESPAASGEPGPSSTATGG
                     VVHVGYKGSPDSLNPGRGLLTESYTIYELVYDTPISIDPEGNYVPELATDWEASEDGL
                     TYTMHLVDDAVFHDGTPMTSEDVKFSLETYRDNDFPYESSYPDVFVDIEAPDPTTVVL
                     HTEEPVGNFEYRMVFMYILPKHIWESEDAFTFDNAAMIGTGSFKLKDYETDEFVELEA
                     VKDHWNISPNIDGVIFQTIDNDDARVAALTEGDVDMITEFQNTAIPTLQNTENVEVVT
                     MEAIGGSLTDLFFNVIDPADCPGPSELVPDGGTCSGHEALRDIEVRQALAKATDKQQL
                     IDVATLGTGTVGVSLVPPGLGDYFVGADAAFPFDVDAANEQLDAAGYEDTDGDGVREC
                     RADQDCDDLTFRFNYPDDSTTGARESELLDAMWSAVGVKIEIQVLDQDALTSVCCPNF
                     DYDVIMWGWGVDPDPQFLLGVTLCENIENGFSETGYCNAAYDELYAAQAQETDHATRV
                     DQIHEMQEILLEDTPYIIPYYYPTINAYRSDTYTGWLTGPRGFGLDDISSLGFIRPAQ
                     "
     gene            12568..>14214
                     /locus_tag="VGO32_04840"
     CDS             12568..>14214
                     /locus_tag="VGO32_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV7200112.1"
                     /translation="MPARRAPTRVTADRLVRAQSVIESFDLTPDGSSIVFGRRVVDGN
                     AYRSHLWVVPVDGGRPRRLTDGAVRDTGPQVSPDGQWVAFVRAASRQPGAKGGKPGDE
                     TGQAWIMSVKRGRPRRLTKLKHGVDSVHWSPDCRSLALLGPAGEPRFVVGPKRDGVEP
                     VARRITRTDWRDDDSGLWERRTHLWVLDATHRGRQGPRQLTEGDYDVANPAWSPDSHW
                     IAFDADMEPDWNIHFRYRIFRVAAAGGSVTELVSLKGDARAPSYSPDGRSIAFLGTDV
                     ENPTVADPERVFLATSDGHRARCLTPDLDDGVGAWAWADLAMAEDMEAPAWEDADHLL
                     TIIGVDGRVRPYRLPRAGGEPEPLVAESARVVAGSVRAHGSHLFVSAAVDGRASELYE
                     LAEGQMRRLTTLGSGWEDRHRQFSLEELEVPGPGGPIQVWVASPRDAGEAPLPTILHF
                     HGGPNGCWAPGGTMDSLLLTAHGYRVAMPNVRGSTTHGSAWIRAHMGTWGETDAADVM
                     AVSDALVERGLADPDRIGLMGLSYGGYLAQWMVCHSDRFAA"
BASE COUNT         2171 a         4861 c         5086 g         2096 t
ORIGIN      
        1 gaccgatggc gcggtcccgg tcgccgaaac ggcacgggca tgaatcgggc gggacacgac
       61 cgatgagcgg aaacgagcgc agaccgcgat tcacgaccca gtccggagtc gagatcaacc
      121 gcctctatac gccggaggac ctggccggct gggatgcggc tcgagacctc ggcgagcccg
      181 gtgggccgcc gttcacgcgc gggattcatg ccaccggcca ccgcggccgg ctgtggacga
      241 tgcggatgtt cgccggcttc ggcgccgcgg aggacacgaa tgcccgcttc cgccggctca
      301 tcgccgaggg cgggaccggg ctgtccatcg cctacgacat gcccaccctg tacggctacg
      361 accacgacga gcccgaggcg gccggggagt tcgggacgtg cggggtcgcg gtcagcagcc
      421 tggccgacat ggagatcctg ctgtccggcc tgcccatcgg cgagatcagc acctcgatga
      481 ccatcaactc gcctgcggcc atgatctggg cgatgtacat cgtggccgcc gagaaggcgg
      541 gcgtgccgcg ggccgagctg accgggacgc tccagaacga catcctcaag gagttcatcg
      601 cccagaagga gtacatcttc ccgccggccc cctcgctccg gctggtgacc gacaccgtcg
      661 aattcgggac gcgcgagctg ccccgctgga acacgatcag catcagcggc taccacatcc
      721 gcgaagcggg ttcgacggcg gtccaggagc tcgcgttcac catcgccgac gggatcgcct
      781 acgtcgagga cgccgtggcc cgcggaatgc gcttcgacga cttcgcgccg cgcctctcct
      841 tcttcttcaa cagccacaac gacttcttcg aggagatcgc gaagttccgc gccgcgcggc
      901 ggatctggta ccgcctgtgc accgagcggt tcggcgccga gaacgagcgc tcgacgtgga
      961 tgcggttcca cacccagacc gcgggtgtgt ccctgaccgc gcagcagcca cagaacaacc
     1021 tgacccgggt cgcgatccag gcgctcgccg ccatcctggg cggcacccag tcgcttcata
     1081 ccgatgcgta cgacgaggcg tgggccgttc cgtcggaagc ggccgccctg ctcgccctgc
     1141 gccagcagca gatcctggcc gaggagtcgg gggtggcgaa cacggtcgac ccgctgggcg
     1201 gttcgtactt cgtggaggcg ctcacgaaca agaccgaggc cgcggcctgg gactacatcc
     1261 ggcgcatcga cgacatgggc ggcatgctcc gggcggtgga ggcgggctac ccccaggccg
     1321 aggtggcgga cgcggcatac gcccagtcgc gcgccctgga atccggggag caccagatcg
     1381 tgggggtcac caggtacgcc gacccgaacg aggagctggc gatcccgctg ctcgagatca
     1441 cggaggaaat ggagcggcgt cacctcgacc gcctgacccg ggtccgcgcc gagcgcgatg
     1501 cggatgcgca cgcgcgggcc atcacccgcc tgcgggagga gtcggcgcgc gacggggtga
     1561 acctcatgcc cgcgatcgtg gccgcggtcg aggcgtatgc cacgatcggc gagatgtgcg
     1621 gcgtcctgcg cgaggtgttc ggggagtacc gcgagccgct cgccgtctga ccggccgtgg
     1681 tcaatgggcc gtaggatcac ggcgtgcagg ttcacctggt tgacgcgacc ttcgagctgt
     1741 tccgggcgta tcactcgcgc ccggccgaga ccggtccgga cgggcggtcg gtgaacgcgg
     1801 tccgcggcct ggtggactcg ctgctgagcc tcctccgcga gccggacgtc acccacgtcg
     1861 gcgcggccac ggaccacgtc atcgagagct ggcgcaacga cgtgtacccc ggctacaagt
     1921 cgagcgcggg cctgccctgg gacctgctca gccagttcga gctggccgag gacgcaattc
     1981 gagccctcgg gatcacctgc tgggcgatgg tcgaagacga ggccgatgac gccctggcca
     2041 cggtcacggc ccgcgtggcc gccgatccgc gcgtcgaaca ggtggtcatc tgcacggtcg
     2101 acaaggacct ggcccagtgc gtgtccggcg agcgcatcgt gcagcgggac cggatgcggg
     2161 gaatcaccta cgacgaggcg ggcgtgatcg ccaagttcgg cgtcccgccg gccagcatca
     2221 ccgactacct ggccctggtc ggggacagcg cggatggata tccggggctg cccgggtggg
     2281 ggtcgaagag cgcggcctcg gtcctcgccc gctgggagca cctggaggcg atccccgacg
     2341 acccgctgac ctgggacgtg gacgtccgcg gcgcgggacg gctggccgtc gccctccgcc
     2401 aggggcggga tgaggcgtat ctctatcggc ggctggcgac cctgaaccgc gactccgacg
     2461 cgcccggcga cgtcgctgac ctcgagtggc atggcgtgcc tcgggcacca ttcgagacgc
     2521 tctgcgccgc gctcggcttc gagtcgattc ccggtcgcgt gcatcgctgg gcctgaccga
     2581 gggggtactc aggggccgga ccacgcccgg accatcggcc cggctgttcc gcggcagacc
     2641 ggccgatacg gtcaggaccg atgccgcgcc gtattgcggc ggcgtcagtg ctggccgcag
     2701 tcgccctgtt cggcagtggg cttcccgggg ccgtcgtccc ggcaagcgcc tcctcgtgca
     2761 gcggctggac cagtgagacg ttgcccccgc ccaccatccg cgtctatcgc gccgcgactg
     2821 gcgcggtaga gaccgtggac ttcagggtct acgcgaagaa cgtgctgtcc cgcgaatgga
     2881 tcggctcatg gacgaccgaa tccctccgtt cgggcgcggt ggcggtgaag atgtacgcct
     2941 ggtaccacgt cctccactgg cggggactga ccaacgccac cggccagtgc ttcgacgtat
     3001 acgacgacac ccgtgaccag gtgtacaacc cggcccagcc gacgtggccg agcgcggcgt
     3061 cggcggtcga tgccacgtgg gccacccggg cgctgaagaa cggtgcgatc tccccgacct
     3121 actacaacgc cggatccgcc aacgaggcgt gcggggcgaa cgcgaatggg tggcggatgt
     3181 accagtgggg cacccaggcg tgcggcctgg ccgggcggac ggcggcccag attctgctca
     3241 cctacttcta tcccggctgg acggtgaccg gtgcgccgcc acccccgccg tcgccgaccc
     3301 cgctgccgac gcctgttccg acgcccgctc ccacccccgc cccgacccca ggcccatcgg
     3361 tacccgcgcc gaccccggcc ccgccgccag cgccgacccc ggcccccaca ccggctcccc
     3421 cgccaccgcc accttccggc gggaatccgg gcggcgggca gtccgggatc gtcggccagt
     3481 ccgcgccgcc gccgcccccg tccagtcagc ccgagccggt gatggtcgct ccggctcccg
     3541 ccgcagccga cgaacctccc gccgatcccg ccccctggct gcgccggccg gcggccagcg
     3601 atcggctctt cgaggggacc gagcgggtga gcaccaagca cctcgcgccg gccgcgccgc
     3661 cgcccgcgtg gtcataccgg gccatggccc cgtcggccat cgccgaagtc gcggtccagc
     3721 tggcgatccc gctgttcatc ggccgcccgg atcccgcgct caacgcaccg cccgtggacc
     3781 tgcccgcggt cgtcggccgc tagtcccttc gagggtcgcg tctaccctcg cccgcctcct
     3841 gcgcgccacg ccgcgaccgc gaggtcggcg gtggcgagat cctgtatcgg caggccaacg
     3901 ctcttgaaga tcgtgatcgc ggcgggatcc cggcactccg cccaatcggc tgcgaccgtg
     3961 ccgatctcca ccaggtccgc ctcgctgatc atcccggccg cgatcgccgc ggccacgtca
     4021 ccggcctcgt gcccgaccgc ctcgcgtgaa tcgaccgcga ccagggacgc gcggccaaag
     4081 gcatcggccg ggatctccac catcccgagc cggaacgcgc cgatggcatt gacgtgggtg
     4141 cccggctcga gccactcggc ctcgaacacc ggcgtctcgg acggggtcac gcagcagacg
     4201 atgtccgcgc cgcgtaccgc ctcctcggcc gagtccgcgg ctcgcacaga ggtgggcagg
     4261 gccacatcca tggcggccgc gaaggcttca cgtggctcgc gggttcgcgc gtagacccgg
     4321 acctcgcgga tgggccgcgc ggcgaggacc gcgcggatct ggggttcggc ctgcacgccg
     4381 gcgccgatga gggccatcac cgacgcgtcg ggccgcgcca acagccgggc accgactccc
     4441 gacgcggcgc cggtccggag tgccgtgacc gccgggccgt cgaactggaa tgccgcctca
     4501 ccggtgaccg cgtcgaacca ggtcaccgtg ccctggatgg tgggcaggcc gcgttccggg
     4561 ttgcccggta cccaggtgat gagcttgacc gcggcccccg atccgccctc ccggaccgcc
     4621 ggcatgagca cgagcaggcc gccgcccgtg agctcgaggt gctggcggag gggcgaccgg
     4681 tcgcggccgg cggccacgtc gcgataggcg gcttcgatcg catcgagcag acggtccatc
     4741 gggaccagcg cacggagggt ggccgcgtcg atcagctcca tcggcggccg agtttccggg
     4801 cgacgcactg cgctagcatc ggtccgccca tggagatcac ggctgtggtg agtccgacta
     4861 gaacagggtg tcgagcgggt ttggcgtacc caggaagaac gacgccccga tgatgatgac
     4921 gagcagcgcg gccgcgaaga ccgagagcca cttcaagact tcccacatgg cccacggatt
     4981 ctagggccat cgcgcaagtg cccaccccgg ccgcccccgt tctgcggctg cgtgacaccc
     5041 ggcgagcagc catccatccg gtggagccgc tgggagcagg cccgatcggt ctgtacgtgt
     5101 gcggcatcac cccgtacgac acgacccacc tgggccatgc gttcacgtac ctcgcattcg
     5161 acgtcatcca tcggtatctc gagtacgcgg gtcacgacgt ccggtacgtg cagaacctga
     5221 ccgacgtgga cgacgacatg ctcgctcgag ccagggacgt gaaggaggac tacctggcgc
     5281 tcggcacccg ccacgccgat cggttcctgg ccgacatggc ggccctcaac tggcttccgc
     5341 ccgacgtcta tgcccgagcc acggagcacg ttccgcaggt cgtggccatg atcgagcggc
     5401 tggaagctgc cgggctcgcg taccgcgggg acgggcacgt ctacctgtcc acgatcgcca
     5461 gtccggattg ggggtcgctc tcccacgtct ctccacccga tcagctcgcg ctggccaacg
     5521 agcgcggcaa ccggcccgac ctccccggca agcgtgaccc gctcgacccg gtcctctggc
     5581 agcgctcgct cgccgatgag ccggcgtggc cgtcgccgtg gggttccggc cgcccggggt
     5641 ggcacatcga gtgctcggcg atgagcgtca cccacctcgg gccgcggttc gagattcacg
     5701 gcggcggtga ggatctcgcg ttcccccatc acgaggcgga gcgaatccag tccgagggcg
     5761 cgaccggcga gcggccagtt gtcggccatt gggtgcacac cgggatggtc cgatacgacg
     5821 cgaacaagat gagcaagtcg ctgggcaacc tggtcatggt cgccgacctc ctggaccgat
     5881 ggcccgccga tgccgtgcgc ctggccctca tccgccattc ctaccgggcc gagctggcct
     5941 ggaccgatgg attggtcgtc gatgcggcag aggtggccgc ccgctggacc accgccgccg
     6001 cccgggctgg cgcggtggag cccgattcac tccccgacga agtggccgcg ggacgggccg
     6061 tcgtgcttga ttcgctggcc gatgacctgg acacgcccgg cgcaatagcc gcactcgacg
     6121 ttctggccgg actcgcgctg gagccggccg acgttgctgc cgcggcgggc tgggtcctgc
     6181 gcgacctcgc gactcggatc ctgggcctgc gactgggcgg aacgcgatga tcgacgacaa
     6241 tcggccgccc agcgtccagc tgggcgaggt cgttccgccc gaggatcccg aggattgggg
     6301 tcagccgctg acctggatcg tggccgctgg catgctcgtc accccgctcg ccggcgcggc
     6361 ctggttcatc ctcgccgccc cgaccgatcc gcggctctcg gtcgaaggta tctcggcgct
     6421 ggcggtgatc ctggccctcg gcgcggccat caccggcgcc actcagcgcg ggatgtggcg
     6481 gacgttcgtg accagcatcg gtgccggcct gttcaatgcg ctcggactgg tggtggtggg
     6541 gaccgtgctc gccggcggtg catcgctcgg cgtggccacc gtcgcggcgg tcgccggcgt
     6601 catcgccacc gtgcccaccg ccatgctggc cgcgctcctc gccgattcgg ccgggcgcgg
     6661 gcgccggatc gtgagcccgg ccctcgcggg cggggtcacc gcgctggtca tcgtccggct
     6721 gatgacgagc ctctgacgat cgggccgctc ccgaccagga tgcaggcggc ctcgtggtcg
     6781 gggccgagcg cgatcagcgc cgggtcgatc gtccggcacg cggcgaccgc caccggacag
     6841 cgaggatgga agcggcagcc ggaagggatg cgagacgggt cgggggtctc gccggcgagg
     6901 acctggggcc gctggcgagc ggcggggtcg gtggtcggca ccaccgacag cagggcccgg
     6961 gtgtacgggt gctggggatc ggacaggatc gtcctggtgt ccccgatctc cacgatccgg
     7021 ccgaggtaca tcaccgccag ccggtcggca taggcggaca ccgtggccag gtcgtgggtg
     7081 atcatcacga tcgcgattcg cgactcggtg gccagccggc ggagcaggtt gaggatgtcg
     7141 gcccggatcg agacgtccag catcgatacc ggctcgtcgg cgatcaggag ccgcggttcg
     7201 agggtcaggg ccgcagcaat caccacccgt tggcgctggc cacccgacag ctcgtatggg
     7261 tagcggttct cgtacacctc tggcggagcg aggccggcct gggtcagggc atgccgcacg
     7321 cgggtctgct cgtcgtcgtc gttgaccgcg acgtgatgga cgcgaagggg ttccgcgatg
     7381 atgtctcgga tccgcatccg cggattcagc gactcgtatg ggtcctggaa gatgatctgg
     7441 caggctcgac ggaacgcccg catctggcgg cgtcccatgc tcgccacgtc ctgcccctcg
     7501 aagcggacgg tcccctcgct gagcgggatc aggccgagca gcaggttgcc ggtcgtggtc
     7561 ttgccgcagc ccgactcgcc gaccagggcc agcacctctc cgggcgagac cgcgaacgac
     7621 acgccatcca ccgcccgcac cgatggcgcc ggtcggcgga gcaggctgtc ggtcagcggc
     7681 cgctgagtgg cgaaccggac atggaggtcg tcagcgacga cgagtggctc agtcatgggt
     7741 ggccggcgaa gcgggcgctg gatgggcggg gtcgaggaga tagcacgacg cggcccaacc
     7801 gtccgagatc gggtacagcg gtggcttggc ggtatcgcat cggtcgaagg cgtgggcgca
     7861 gcggggagcg aatggacagc cgggcggcat ggcgtccaac cgcggcgggg tgcccccgat
     7921 ggcatgcagc tctcgctcgg gatgggccac gtcggggaag gcctcgagca gggcctgggt
     7981 gtatgggtgg cggggctcgg cgaacaggcg gctggcgggg gcgtcttccg ccacgcgccc
     8041 tccgtacatc accaccacgc ggtcgcagac ctgggccacg atccccaggt cgtgggtgac
     8101 gatgatcgtg ctcatcccga ggtcgcgcga caggccgccc agcagctcca tgatctgagc
     8161 ctggatcatc acatccaggg cagtcgtcgg ctcgtcggcg acgatgacgt ccggccggca
     8221 ggccagggcg agcgcgatca tggcgcgctg ccgcatcccg cccgagtagg tgtgggggta
     8281 ctcgttggcg cgacgggcgc cgatcccgac ccgatcgagg agatcggcgg tgcgccggtt
     8341 ggcctcgtcc ttcgagatcc ggtgatcatg gagtcggatg gcttccgtga cctggccacc
     8401 gacggtctgc accggattca gggcgttcat ggccccctgg aagaccatcg acaggcgcgt
     8461 ccagcggaac gcccgcagct cgtcgggcgg cagggacagg agatcctccc catcgaagag
     8521 cgcctgcccg gccacgatcc gcccgcccgg gggcaggagg cggaggaggc cgagcatggc
     8581 cgtcgtcttg ccgctgcccg actcgccgac caggccgatc gtctcgccgc gggcgaggtc
     8641 gaagctgacg tcgtccaccg cccggagtgg gccgtccgat gactcgaact cgacgctgag
     8701 gccccggatc gagagcagcg gcgcgtttgc cgtgggaatc atcaccgccg ggggcggctc
     8761 caccgagcgg acgacgtcgt cccgcccggc ctcgaggtga tggcgcttga gccgcgggtt
     8821 caggacgtcc tcgagggcgg ttccgagcag ggtggtggcc agcaccaccc acacgatggc
     8881 caggcccggc gggaagaagg cccaccatgc cccggccgag accgcgccac gatcgaacgc
     8941 gaagttcagc atcttccccc agctgatcac ggtcgggtcg ccgaggccga tgaacgccaa
     9001 ggtcgactcg gacaggatgg ccaggctgat gaccaggacc gcgttggcca gcatcagggg
     9061 catgaccagc gggatgatgt ggcggcggat catgtgccaa tcggaggccc cgatggcgtg
     9121 cgcccgggcg acgaacttcc gctcacggac ggtcagcgcc tgggagcgga cgagccgcgc
     9181 cgtcgtcgtc cagcccagga tcccgatgac gatgatgatc gtcaccaggc tcggacccag
     9241 gatggcgacc agcacgatct ggagcgccag gtcggggatg acgaggaaga agtcggtgaa
     9301 gcgcatcagc gcgctgccgg tccggccggc cttgtagccg gccacgatcc cgatcgctcc
     9361 gccgatgacg atcgcgatgg cggcggccgt gaagccgacg atcagggaca cgcgcgagcc
     9421 gtagatcagc tcggagagga cgtccttgcc gccgtcatcg gtcccgagtg ggtgctcggg
     9481 cgagggcggc tggtagatgt cctcgatgtg aaccggcggg gtgtcgtagg ggtcgtaggg
     9541 cgccagcaac ggggcgaaga cggcgacgaa gaaggtcagc ccgagcatga tcaggccggc
     9601 caggccgacc gggtggtgga gcagggtccg cagcgccccg cgcggcgccg cgatggtcgt
     9661 cgggcggctc tggacctcgg cgacgacggt catcgatcag gcctcggtga cccgggggtc
     9721 caggtagccg tacacgatgt ccgcgaccag gttggcgaac accaccgcca gggcgaggag
     9781 caggaacgca ccctggagga ccgggtagtc gcgatcggac accgcctgca ccgtgagatg
     9841 gcccaaaccg ggccaggagt agaccgattc aacctggatc gcgcccgaca ccgtgaaggc
     9901 gaggttcagg gcgatgaggg tgaccatggg cagcatcgcg ttcttcaggg cgtggccgcg
     9961 gatgatccgg taggtggaca ggcccttggc cttggcggtc aggacgtagt cctcgctgaa
    10021 gacgtcgagc acgctcgacc gcatgatcag catgtactcg ccgagcagga ccagggtgag
    10081 ggtcagggtc ggcagcagga gatggcggaa caggtcgacg gcggcctcga ccggggtttc
    10141 gaacgccgcc ccgatcgtgc ccttgccgcc gatcggcagg tcgaaatacc gagccccgat
    10201 gaacagcagg atgatggcca ggaagaacgt cggcagggac cacatgaaca gcgagaacgc
    10261 gagcgagccg atgtcggtcg ccttgccccg gcgccaggcc gcgaccaggc cgaggatgat
    10321 cccgaacagg atcgacagga actgggatgg cagcacgagc cacagggtgt tgaccagcgc
    10381 gtcgcccagc tgctccgcca ccggatcatc ggggcgggca taggaggttc ccaggtcgcc
    10441 ctgcatcagg gcgccgatgt aaatgaagta ctgggagtcg aacggcgccc cgctcccgaa
    10501 gttgaagaac aggggcttgt cgaggccgaa ccgttcggtc agcgcggcct gcgtctcggg
    10561 attgagccgc gggtcccggg ctccgcttct ggccgggtca ccggggagca cccggaacag
    10621 gacgaagttg aaggtgatga ccacccagac ggtgaccagc gcccagccga cccggcgcgc
    10681 caggaaccgc gtgcggccgc tcattcagcc gacatccgtc gccgtccgcc gatcgaggct
    10741 gcggccgtcg cggccggtcg tattactgcg ccgggcggat aaagccgagc gaggagatgt
    10801 cgtcaaggcc gaagccgcgc gggcccgtca gccagccggt gtaggtatcg gaccggtagg
    10861 cgttgatggt cgggtagtag tacggaatga tgtagggggt gtcctcgagc aggatctcct
    10921 gcatctcgtg gatctggtcg acccgagtcg cgtggtccgt ttcctgggcc tgggccgcat
    10981 acagctcgtc gtacgccgcg ttgcagtacc cggtttcgct gaacccattc tcgatgttct
    11041 cgcacagcgt gacgccgagc aggaactgcg gatcggggtc gaccccccag ccccacatga
    11101 tcacgtcgta gtcgaagttc gggcaacaga ccgaggtcag ggcatcctgg tccagcacct
    11161 ggatctcgat cttgacgccc acggcgctcc acatcgcgtc gagcagctcc gattctcggg
    11221 cgccggtcgt gctgtcgtct gggtagttga accggaaggt gagatcgtca cagtcctggt
    11281 cggcccggca ctcccggacg ccgtcaccat ccgtgtcctc gtacccggcc gcgtcgagct
    11341 gctcgttggc cgcgtcgacg tcaaacggga acgcggcgtc ggcgccgaca aaatagtcgc
    11401 ccagcccggg cgggaccagt gacaccccca ccgtcccggt gcctagggtg gcgacgtcga
    11461 tgagctgttg cttatcggtc gccttggcca gtgcctggcg cacctcgatg tcgcgcagcg
    11521 cctcgtggcc ggagcatgtg ccgccgtcgg ggacgagctc gctcggtccg gggcagtccg
    11581 ccggatcgat gacgttgaag aagaggtcgg tcagcgagcc gccgatggcc tccatggtca
    11641 cgacctcgac gttctccgtg ttctggagcg tcgggatggc ggtgttctgg aactcggtga
    11701 tcatgtccac gtcgccctcg gtcagggcgg ccacccgggc gtcgtcgttg tcgatggtct
    11761 ggaagatgac tccgtcgatg ttcgggctga tgttccagtg gtccttcacc gcctccagct
    11821 cgacgaactc atcggtctcg tagtccttca gcttgaagga gccggtgccg atcattgccg
    11881 cgttgtcgaa ggtgaatgcg tcctcgctct cccagatgtg cttggggagg atgtacatga
    11941 acaccatccg atactcgaag ttgcccaccg gctcttcggt atggaggacc acggtcgtcg
    12001 gatcgggcgc ctcgatatcg acgaagacat ccgggtagga cgactcgtag gggaagtcat
    12061 tgtcgcggta ggtctcgagc gagaacttca cgtcctcgct ggtcatcggc gtcccgtcgt
    12121 ggaagaccgc gtcgtcgacc aggtgcatgg tgtaggtcag cccgtcctcg gaggcctccc
    12181 agtcggtggc cagctcgggc acgtagttgc cttcgggatc gatcgagatc ggggtgtcgt
    12241 agacgagctc gtagatcgtg tacgactcgg tgaggagtcc tcggccgggg ttcaggctgt
    12301 caggcgaacc cttgtagccg acgtgcacga cgccgccggt cgcggtgctg gacggcccgg
    12361 gctcgcccga ggcggcggga ctctcgctgg ccgatccctg gcaggaggcg aggaccatgg
    12421 tgagtccgat gagggcggcg gcggggcgaa gcgggcgacg cacgagggag gcacctctta
    12481 acgggacgct aacgggacgg tgatgatggc acacggcttc tcgccgaggc aatggcgcga
    12541 cgatggccac ggcatactcc tcgggccatg ccagcccgtc gagccccgac tcgggtaacc
    12601 gcggatcgcc tcgttcgcgc gcagtcggtg atcgagtcgt tcgacctcac gccggatggg
    12661 tcgtcgatcg tgttcggccg gcgcgtggtg gacggcaacg catatcggtc ccacttgtgg
    12721 gtcgtcccgg tcgacggtgg tcgccctcgg cggctgaccg atggggccgt ccgggacacc
    12781 gggccacagg tttcgcccga cggccagtgg gtggcattcg tccgcgccgc atcacggcag
    12841 ccgggggcga agggcggcaa gccgggtgac gagaccggcc aggcctggat catgtcggtg
    12901 aagcggggcc ggccacggcg cctgacgaag ctcaagcacg gcgtggactc ggtccactgg
    12961 agtcccgact gccgctccct cgccctgttg ggtcccgccg gtgagccacg attcgtggtc
    13021 gggcctaagc gtgacggcgt ggagccggtg gcccgccgga tcacccggac ggactggcgc
    13081 gacgacgact cgggactctg ggagcggcgg acccacctgt gggtgctcga cgccacgcat
    13141 cgtggccgcc agggcccgcg ccagctgacg gagggcgact atgacgtcgc caatccggcc
    13201 tggtcaccgg acagtcactg gatagccttc gatgcggaca tggagccgga ctggaacatc
    13261 cacttccggt accggatctt ccgagtcgcg gccgcaggag ggtcggtgac cgagctcgtg
    13321 agcctgaagg gtgatgcccg cgccccttcc tattccccgg atgggcggtc gatcgcgttt
    13381 ctcgggacgg acgtggagaa cccgaccgtc gccgaccctg agcgcgtctt tctggccacg
    13441 tctgatgggc accgggcgcg ctgcctcacg ccggacctcg acgacggcgt cggcgcgtgg
    13501 gcgtgggccg acctcgccat ggccgaggac atggaggccc cggcctggga ggacgcggac
    13561 cacctcctga ccatcatcgg cgtcgacgga cgggtgcgcc cgtaccggct cccacgtgcg
    13621 ggtggcgaac cggagccgct ggtcgccgaa tcagcgcggg tggtcgctgg ttcggtgcgc
    13681 gcccacggta gccacctctt cgtcagcgcg gcggtcgacg gccgggcatc ggagctctac
    13741 gagctcgccg aggggcagat gcggcggctg accaccctcg ggtccggttg ggaggatcgc
    13801 caccggcagt tctccctcga ggagctcgag gtcccgggac ctggcgggcc gatccaggtc
    13861 tgggtggcgt cgccgcgcga cgcgggcgag gcgccgcttc ccacgattct ccacttccat
    13921 ggtggcccga acggatgctg ggcacccggc ggcacgatgg attcgctcct cctcaccgcc
    13981 cacggatacc gggtcgcgat gccgaacgtc cggggctcga cgacccatgg atcggcctgg
    14041 atccgcgccc acatgggaac ctggggggag acagacgcag cggacgtgat ggcggtttcc
    14101 gacgcgctcg tcgagcgcgg gctcgccgac ccggatcgga tcgggctgat ggggctctca
    14161 tacggcggct acctcgccca gtggatggtg tgccacagcg accggttcgc cgcc
//
LOCUS       DASYGU010000175         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1623419, whole genome shotgun sequence.
ACCESSION   DASYGU010000175 DASYGU010000000
VERSION     DASYGU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623419"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGO32_04845"
     CDS             complement(<1..670)
                     /locus_tag="VGO32_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEV7200113.1"
                     /translation="MSDWPVVGVAGARGMVGGVFLSILADAGLPAGSLRAFGTDVGAV
                     AYDGTDLPVGVMDDGAADGLDVLFLATDGDTSRSLVEGPARTVPLVVDNSSAYRLDPA
                     VPLVVPEANGAVLAGYGGRLVANPNCTTAAAVVALAPIRDLAGLESVDLASYQAVSGA
                     GRAGVDAFVAERATDTREAAAESPFHGRIADSVVPQIDRLFDDGWTGEERKVIAELRK
                     ILDLP"
     gene            complement(663..1607)
                     /locus_tag="VGO32_04850"
     CDS             complement(663..1607)
                     /locus_tag="VGO32_04850"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV7200114.1"
                     /translation="MEPLRIGLVGWGTVGSALGELIERGPLPIAISWVAVREPRRERG
                     VPLPEGVPVSGEPGPMDEIDALVELAGSVTEPRRWAEQALEAGLPYVTANKALLAAHG
                     AELADMALLHDAALLASASVGGGTPMLEVTEHLAASGRVTHLRGLLNATTTFILSAMA
                     DGQTYRDALGEAQSAGYAEADPSFDVDGRDAAQKLAILAWVAWSTWIPEHEVETRGIR
                     DVAVAPGSTVRLVADATPGSLRVSPTVLPANDPLAGASGIECVLEIHSDAAGPLTIRG
                     PGAGGPPTAGAVYADLARLARGERPILGQRPAGSPVRV"
     gene            1634..1969
                     /locus_tag="VGO32_04855"
     CDS             1634..1969
                     /locus_tag="VGO32_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200115.1"
                     /translation="MGATETIDLFLELINEDQRERAVDLFAENAIMGISTPGSDERTN
                     LRGRDRVGGWFIRAGDGFRMYVNDSRNMGASLIADVTVIRPGIDPLHVEANFRVENGE
                     IVSLFLQPN"
     gene            complement(1980..2750)
                     /gene="cofE"
                     /locus_tag="VGO32_04860"
     CDS             complement(1980..2750)
                     /gene="cofE"
                     /locus_tag="VGO32_04860"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEV7200116.1"
                     /translation="MSPVEIHAIDGLPEVSPGDDLGALVAAAVDAAGIGLTDDDVLVV
                     TQKVVSKAEGRVVDLASVEPGDEARDWAERWDRDARQVELVLRESAEVIRWADGGLII
                     SRTRHGFVCANAGVDVSNTGIETGERATLLPEDPDASARRIRQRVGEVSGAHPAVLVT
                     DSFGRPWRDGITDVTIGAAGIDVLADMRGTPDADGRELHATVIAVADEMASAAELAAG
                     KASGRPVVVIRGYRYAHPAADDPGARPLVMDRGRDLFP"
     gene            complement(2747..3346)
                     /gene="cofC"
                     /locus_tag="VGO32_04865"
     CDS             complement(2747..3346)
                     /gene="cofC"
                     /locus_tag="VGO32_04865"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEV7200117.1"
                     /translation="MSTRIIVPHRGLEHAKTRLAGVLEPAERSAVAERLLRHVLEAAV
                     GTGHEVVVISPEAALEELVVANGARLAVQHGLGLNSGLREARTEAIEDGVTVLAVIHG
                     DLPELTREDMAALIDAVGGAGRTVAIAPDALEAGTNGLAQSPPDVIAFQFGVGSFAAH
                     RGSAIRAGAHAVVIERPGLAFDVDTPADLAAWVARGFAA"
     gene            complement(3356..>3617)
                     /locus_tag="VGO32_04870"
     CDS             complement(3356..>3617)
                     /locus_tag="VGO32_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEV7200118.1"
                     /translation="SPIVAGRALKGPTDRMLASLGHDVSATGVARLYEGLLDRYVLDE
                     ADAGLAAEVTALGMGAVVAQTVMETDADRAGLARFLLESPAR"
BASE COUNT          521 a         1374 c         1251 g          471 t
ORIGIN      
        1 cgggcaggtc caggatcttt cgcagctcgg cgatgacctt ccgttcctca cccgtccagc
       61 catcgtcgaa caggcggtcg atctggggca cgaccgagtc ggcgattcgt ccgtggaacg
      121 gcgactcggc ggctgcctcg cgcgtgtcgg tggcgcgctc ggcgacgaag gcatccacgc
      181 ccgcccgtcc cgccccgctg acggcctggt acgaggccag gtccaccgac tcgagcccgg
      241 ccaggtcgcg gatgggggcg agggctacga ccgccgccgc agtcgtgcag ttcgggttgg
      301 ccaccagccg cccgccgtag ccggccagca cggcaccgtt ggcctcgggc acgaccagtg
      361 gcacggcggg gtcgaggcgg tacgcgctcg agttgtcgac caccagcggg acggtccggg
      421 ccgggccctc gaccagtgag cgcgatgtat cgccgtcggt ggccaggaag agcacatcga
      481 ggccatcggc cgcgccatcg tccatcaccc cgaccggcag atcggttccg tcgtaggcga
      541 ccgccccgac atcggtcccg aaggcgcgca gcgatccggc aggcagcccc gcatcggcca
      601 ggatggacag gaagacgccg cccaccatac cgcgggcgcc ggcgacgccg accaccggcc
      661 aatcagacac gaaccgggct cccggcgggc cgctggccaa ggatcggacg ctcgccccgt
      721 gcgagccggg ccagatcggc gtacaccgcg ccggccgtcg gaggaccgcc tgctcccggg
      781 ccacggatcg tgagcggtcc agcggcatcg ctgtggatct cgagcacgca ctcgatgccc
      841 gaggcgccgg ccagcgggtc gttggcgggc aggaccgtgg ggctcacccg gaggctgcca
      901 ggcgtggcat cggccacgag tcgtaccgtc gagccggggg cgaccgccac gtcgcggatg
      961 ccgcgggtct cgacctcgtg ctcggggatc caggtgctcc acgccaccca ggccaggatc
     1021 gcgagcttct gcgccgcgtc gcgtccgtcc acgtcgaacg agggatccgc ctcggcgtag
     1081 ccggccgact gcgcctcgcc cagggcgtcg cggtaggtct ggccgtcggc catggccgac
     1141 aggatgaacg tggtggtggc gttgaggagg ccgcgaaggt gggtcacgcg gccggatgcg
     1201 gcgaggtgct cggtgacctc cagcatcggg gtcccacccc ccaccgaggc agaggccagc
     1261 agggccgcgt cgtggaggag ggccatgtcc gccagctcgg cgccgtgggc ggcgagcagc
     1321 gccttgttgg cggtcacgta cggcaggccc gcttcgagcg cctgctcggc ccagcgacgc
     1381 ggctcggtca cgctgcccgc cagctcgacc agggcatcga tctcgtccat cggtcccggc
     1441 tcgccggaca ccggcacgcc ctcgggaagg ggcacgccac gctcgcggcg cggctcccgc
     1501 accgcgaccc acgagatggc gatgggcagc ggcccacgct cgatgagctc acccagcgca
     1561 gagcccaccg tcccccagcc caccaggccg atgcggagcg gctccatgcg acccacggta
     1621 tcctgcgcgg gccatgggcg ccaccgagac gatcgacctg ttcctcgagc tgatcaacga
     1681 agaccagcgc gagcgtgccg tcgacctgtt cgccgagaac gcgatcatgg gcatctccac
     1741 ccctggctcc gacgagcgga ccaatctgcg cggccgcgac cgcgtgggcg gctggttcat
     1801 ccgggccggc gacggcttcc ggatgtacgt gaacgacagc cgcaacatgg gcgcctcgct
     1861 catcgccgac gtgaccgtca tccggccggg catcgacccg ctccacgtgg aggccaactt
     1921 ccgggtggag aacggggaga tcgtcagcct cttcctccaa cccaactgag gcgcggcggc
     1981 taggggaaca agtcccggcc gcggtccatg accagcggtc gggcaccggg gtcatcggcc
     2041 gccgggtggg cataccgata gccccggatc accaccaccg gccggcctga cgccttgccc
     2101 gccgccagct cggcggccga tgccatctcg tccgcgaccg cgatcaccgt cgcgtggagc
     2161 tcgcggccgt cggcgtccgg cgttcctcgc atgtcggcca ggacgtcgat cccggccgcg
     2221 ccgatggtca cgtccgtgat cccgtcccgc cacggccggc cgaacgagtc ggtgaccagc
     2281 acggccgggt gcgcgccgct gacctcgccc acccgttgcc ggatccgccg cgcggacgca
     2341 tccgggtcct ccgggagcag cgttgcccgc tccccggttt cgatccccgt gttcgagacg
     2401 tcaacccccg cgttcgcgca cacgaacccg tgacgcgtcc ggctgatgat cagccccccg
     2461 tctgcccagc gaatgacctc ggccgattcg cggaggacga gctccacctg ccgcgcgtcg
     2521 cggtcccagc gctcggccca gtcccgggcc tcgtcaccgg gctcgactga cgcgaggtcc
     2581 acgacccggc cctcggcctt ggacacgacc ttctgggtca cgaccaggac gtcgtcatcg
     2641 gtcaggccga tcccggcggc gtccactgcc gccgcaacga gcgcccccag gtcatcgccc
     2701 ggcgagacct cgggcaaccc atcgatggca tggatctcga ccgggctcac gccgcgaacc
     2761 ctcgggccac ccaggcggcc aggtcggcgg gcgtgtccac gtcgaacgca agccctggcc
     2821 gctcgatcac gaccgcatgc gccccggccc gtatggcgga tccgcggtga gccgcgaagc
     2881 ttccgacccc gaactgaaat gcgatgacgt ccggtggcga ctgggcgagc ccgttggtcc
     2941 cggcctcgag cgcgtcgggg gcgatggcca cggtccgtcc tgccccgcca accgcatcga
     3001 tcaacgcggc catgtcctcg cgggtcagct cgggaagatc gccgtggatg acggccagca
     3061 cggtcacccc gtcctcgatg gcctcggtcc gcgcctcgcg aagcccggag ttcaacccca
     3121 gcccgtgctg gacggcgagc ctggcgccat tcgcgaccac gagctcctcg agcgccgcct
     3181 cgggtgagat gaccacaacc tcgtgccccg ttcccacggc cgcctcgagg acgtgacgca
     3241 gcagccgttc ggccaccgca gagcgttcgg ccggctcgag cacgccggcc aggcgggtct
     3301 tggcgtgctc cagcccgcgg tgtggaacga tgatccgggt gctcaccgcg gcagcctagc
     3361 gcgccggcga ctccagcagg aagcgggcca ggccggctcg gtcggcatcg gtctccatga
     3421 ccgtttgggc cacgacggcg cccatcccca gcgcggtgac ctcggccgca agcccggcat
     3481 cggcttcgtc caggacgtag cgatccagca ggccctcgta cagccgcgcg acgccggtgg
     3541 cgctcacgtc gtggccgagc gaggccagca tccggtcggt cggccccttc agggcacggc
     3601 cggcgacgat gggactg
//
LOCUS       DASYGU010000176        11018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_491706, whole genome shotgun sequence.
ACCESSION   DASYGU010000176 DASYGU010000000
VERSION     DASYGU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11018
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491706"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VGO32_04875"
     CDS             complement(<1..952)
                     /locus_tag="VGO32_04875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7200119.1"
                     /translation="MTDVLILSAARTPIGKFGGGLSTVPATDLGAVAIRAAVERSGVD
                     PTFVDEVFMGQVIQAGAGQAPARQAARKGGLPDGVSATTINKVCGSGLKAVMLGAASI
                     RAGDANVIVAGGMENMNLGPYLLPGARAGYRLGNQELVDATVYDGLWCSACDVHMGIH
                     AERVAAKHNVSREAQDAFALRSHQRALAAQVSGAFTDEIVPVEVPGKKGPIIVDTDEG
                     PRADTSAEALANLKPAFQTDGDGTVTAGNSPGITDGAAALVLASSEAVERDGLTPMAR
                     ITGYAQADVAPEWLFEAPIEGVQRLVDKVGIPLADFDLIEI"
     gene            1125..1667
                     /locus_tag="VGO32_04880"
     CDS             1125..1667
                     /locus_tag="VGO32_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7200120.1"
                     /translation="MDLRIVERAQQGDQEAFAEIAHAIAARLYAVAYRILRDHDRADD
                     AAQHAIVAIWRDLPGLRDPDRFEAWAYRILVNSCYAEARRAKRAHEIPGFGADPTAPD
                     DVMTVADRDQLERGFSRLSADQRAVLVLQHYLDLGLPEIAELLGVPLGTVKSRSHAAR
                     QAMRAALEADARQAKGAQPA"
     gene            1745..3025
                     /locus_tag="VGO32_04885"
     CDS             1745..3025
                     /locus_tag="VGO32_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200121.1"
                     /translation="MPDPSLDAALRAVAVTPQRHWRGRLVRFAGASPLVPVGLTLVAF
                     AAVVTLGIGFTQVLPIPGTEATPTPGFTDTPTPPPTNRPSGSVVYTNEEDGYELTLPA
                     RWTPDDFFSNPPGTMRFGAAHSDGDPSGFGALTVSIGSTDGTVYDCAPSLCRPIVATT
                     IDELDAAVENDSIVVETPMITTVDVDVTLDGEPARLEYVTFAGGLIEQGAQFHHLFAI
                     HNGRPVVLSFDKYAYGLGQESNDIGWVEALIQGFRFLNRHEGPSPNPDDSDQTTWKTV
                     ELANAGFTVAIPSDWVVDAGDDPAVVTLRGTSGSVRVRAGDQSGHISMCLTLAPCQLV
                     EARSMDDLIRVVRGEYRVHWLSEYNLTDERVIVDGVDAARVTLSPRGGISGPGTSRYL
                     VFVRDERAVILEWRPTFSGTGLWNEIISGLRFID"
     gene            3129..3674
                     /locus_tag="VGO32_04890"
     CDS             3129..3674
                     /locus_tag="VGO32_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV7200122.1"
                     /translation="MERRLVELAQRGDQKAFSEIAFSVSPRLFPVAQRILRDYQRAED
                     ATQQTLVQIWRKLPNLSDPDKFDGWAYRILVNTCYAEARKNKRHPDDLRLLETDRASA
                     DHALSVADRDMLERAFDRLPAEQRAVLVLQYYLDLGHPQIADMLGVPLGTVKSRASSG
                     RLALRAALEADARAGIGGVAR"
     gene            3671..5329
                     /locus_tag="VGO32_04895"
     CDS             3671..5329
                     /locus_tag="VGO32_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200123.1"
                     /translation="MTDRGRPMTESELDRLLGTWLAEGAAATPDRIAEHAMLEIATTP
                     QEGTRWSLFWASMTSAPVAWSAVAILAVAVGLAILVGPRLAEPTPTPTPSPSASPTAS
                     PGATATHQSATAVGRIDWVRIESERRVFPTSPYGGQILGWEPTEDFEVAGVYLSMDGT
                     NWQPTDAPPWGLSPEFLNRDNPTQVAYSPDGYGTSMGPDSSRQIIYRDWTDNPGDPVI
                     FQLDRDRWLPIVLPSTAPPPTEGLRIYGSRIQGAAALDESNWIAPVMHFVEVPWADIL
                     GGDLADAWPMWKEDAQRLEIHEPGGDPFGSAGLVNLTVELVEGDPPVIEFRDSETGDL
                     VHVVPATLPGWSPEALVTALRGWGLLDYSFVVSRNGEIRVVRPPWTMGEDWTDIRVVT
                     AFGRYYTITLPLGEGYSSTDIHLWESEDGISWTLLDTPQLWTGTLEWAEIVGGNDQLL
                     IIPHDFEGSSPMSVSADGRTWSEAVLGDSTAGLFSPTSTDFGWLGNGFDQTAISADGM
                     NWEWIDLPDLPAEPSVTYINGIFFYGPEDVGNGRFATWVGHLAD"
     gene            5793..7106
                     /locus_tag="VGO32_04900"
     CDS             5793..7106
                     /locus_tag="VGO32_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200124.1"
                     /translation="MVVDVLDDFKNTFCDGCLASSRPDSLAGEAASRVRISGYEYVAA
                     LHDGRPYVVRFRMTYAFQPSAPGVPSWESILDTFQFVDAHAGSVFQRELQLAGLSLAL
                     PEGWERCHETNEFVFRPAAACAEAASRPYWVNSTPEPSVTVRLGTTGEPPTYCVGVDS
                     ACQYPASGECQTTGACTDVDVASLSNLWSALSREDALRWADSEQRDIEIDGGPGRVVH
                     VSRELREGEITYEKTWLLTIRERPYVLIFAAPSGSMPDGWVQDIISSADFANPPGGQS
                     TWETFTAAEAGFAIDLPQDWVEEPTNDPQLVWVHGGGGGLRVRVGDETGEILNCDIPA
                     GSTCQSVHVESVETLVDLMTPPPGTTFYGPGTANMNRRPVLLGSIPAVQVRIATWAVG
                     PSAHPTTRYYVVAVVGGRPVIIKWEPGSLHGVGFQRLLEGFRLLN"
     gene            complement(7134..9059)
                     /locus_tag="VGO32_04905"
     CDS             complement(7134..9059)
                     /locus_tag="VGO32_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7200125.1"
                     /translation="MTTTATPTPSAATGPAATPAGGKLMRNEIEALLLPESEKARNVV
                     ELIHRSVERIPDKEALRWKLPKDGGWRSNSYAEFWDRITRTSLGLKGLGVKDSDSVCI
                     MSRTRPEWLVADIASWALGAVSCPIYPQTEAGQAAFILNNVGAVAVFVENAQQANKVE
                     SIRSQVPTLRHVITFDTSGKPPAGTLTLEELSGREPDEAQRAAWAAGWRAIDREHLAT
                     VIHTSGTTAQPKGAMIRHGNLLYNYEAAVQIVDFYEADIFLSWLPLSHIFERLTGEII
                     PLAHGSTVVYAESLIERLADNMVEVQPTVMAAVPRFYERVYGRVLASVESSPPMRKRI
                     FHWGIDVGRKRYENHVAGRADGLGLKVQHAIADKLVYHRVKARTGGRVRYFVSGSAPL
                     SREVGEFFYALGILILEGYGLSETSPLVSINRPDDFVFGTVGRPAPATEVRIDPETGE
                     IQVRGPQIMLGYLNQPDETAKAIDPDGWFHTGDVGELDPIGRIKITDRLKNIIVLANG
                     KNVSPGPMEAALSASKYIAQAVILGDRHPYTGALIAPNFDELKPWAEANGLDGMEPEQ
                     LIEEKAVRQLIEGDVRDLLEDFAVFERPRRIALLPRALSEENGELTPTLKTKLRVVQE
                     NWPAQIDRLFEDDGVPA"
     gene            complement(9095..9967)
                     /locus_tag="VGO32_04910"
     CDS             complement(9095..9967)
                     /locus_tag="VGO32_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV7200126.1"
                     /translation="MSLERVYVLGAGLMGHGIAQVSAAAGKQVTIADRTEDLAIAGRQ
                     RIAGNLARLVEKGPLAQDEADATLGRISTAVGVDGVAGHDIVIEAVFEDEAIKRESWT
                     AVTDAADLDAIFASNTSSISITALATATDRPDRFIGLHFFSPVPVMKLIEIIRGLETS
                     DQTEQACRAFADELGKTVITSKDYPGFLVNRMLGPFINEAVFALAEGTGTADEIDTGA
                     RLGLNHPMGPLELADFVGVDVMLNVMDVLYRGFGDPKFRAAPLLRQMVAGGHLGRKTG
                     RGFYRYDEQGNKIG"
     gene            complement(10008..10919)
                     /gene="meaB"
                     /locus_tag="VGO32_04915"
     CDS             complement(10008..10919)
                     /gene="meaB"
                     /locus_tag="VGO32_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV7200127.1"
                     /translation="MADLAERLREAQEGDRLALARLLTDLEDGALDASDRGAVFAAGH
                     GAHLVGITGPPGSGKSTLVDALIRAWRAQGRRVGVIAVDPSSPLTGGAIMGDRIRMMD
                     HVGDDGVFVRSMASRGALGGLAATTWLTAAALDAGGYDPVLIETVGAGQSEVEIATLA
                     ETTVVVEVPEMGDEVQAIKAGLLEIADVVVVNKSDRPGADQAARQLKAMLSTAGGRVA
                     RPAPPVLLTSATTGEGVETLREAIEAHRRQRTPERDLARATELLRRALASRAHDRALH
                     APGWDDAVAAVAERREDPFAAADRLLG"
BASE COUNT         1723 a         3732 c         3734 g         1829 t
ORIGIN      
        1 taatctcgat caggtcgaag tcggccagtg ggatgccgac cttgtccacc aggcgctgga
       61 cgccctcgat cggggcctcg aacagccact ccggggcgac gtcggcctgg gcatagccgg
      121 tgatccgtgc catgggcgtc aggccgtccc gctccaccgc ctccgacgag gcgaggacga
      181 gggccgcggc gccatcggtg atgcccggcg agttgcccgc agtgacggtt ccgtcgccat
      241 cggtctggaa ggcaggcttg aggttagcca gggcctcggc agaggtgtcc gctcgcgggc
      301 cctcgtcggt gtccacgatg atcggtccct tcttgccggg cacttcgacc gggacgatct
      361 cgtcggtgaa agcgccggac acctgggcgg cgagcgcgcg ctggtgggag cggagggcga
      421 acgcgtcctg ggcctcgcgg ctgacgttgt gcttggccgc gacccgctcg gcgtggatgc
      481 ccatgtggac gtcgcaggcg gagcaccaca ggccgtcgta cacggtggcg tccaccagct
      541 cctggttgcc gagccggtag ccggcccggg cgcccggcag caggtacggg ccgaggttca
      601 tgttctccat cccaccggcc acgatcacgt tcgcgtcgcc ggcccggatg gatgccgcgc
      661 ccaacatcac ggctttcagg ccggagccac agaccttgtt gatggtcgtc gcgctgaccc
      721 cgtcggggag gccccccttg cgcgccgcct ggcgagcggg cgcctggcct gccccggcct
      781 ggatgacctg gcccatgaac acctcgtcca cgaacgtggg gtccacgccc gagcgctcga
      841 ccgcagccct gatggcgacc gcgccgagat cggtcgcggg cacggtggag aggccgcccc
      901 cgaacttgcc gatcggcgtc cgggcggcgg acaggatcag gacgtcggtc acgccgacat
      961 ggtacccggg gtgctgaggc cagcgggata cccacctcgc tcagaacgag acgtgactga
     1021 ggccgcagac gacttcgtcg agccgataat cgaaccgcgg ctggcccatg ctgactcctc
     1081 ccagtgagcg gacctgccca agagggccgc accgaggtcg atgagtggac ctgaggatcg
     1141 tggaacgggc gcagcaaggc gaccaggagg cattcgccga gattgcccat gccatcgccg
     1201 cacggctcta tgcggtcgcc tatcgcatcc tccgcgacca cgaccgagcc gatgacgctg
     1261 cccagcacgc catcgtcgct atctggcgcg acctgccagg acttcgcgac cccgatcgat
     1321 tcgaggcctg ggcgtaccgg atcctcgtca acagctgtta cgccgaggca cgccgcgcga
     1381 agagggcgca tgagattccc gggttcggcg cggatcccac ggcgcccgat gacgtgatga
     1441 cggttgccga ccgagaccag ctcgagcgcg gattcagccg tctgtctgcg gatcaacgcg
     1501 ccgtgctcgt tctccagcat tacctcgacc tggggctgcc cgagatcgcc gagctgctcg
     1561 gcgtcccgct gggcacggtg aagtcccgga gccatgccgc ccgtcaggcg atgcgcgctg
     1621 cgctcgaagc cgacgctcgc caagccaaag gagcccagcc ggcatgagtg gcaacggccg
     1681 actctttacc gatggcgccg tcgatcgcgt gctcgagcgc tggctttcgg acggtcctac
     1741 tgacatgccc gatccctcgc tcgacgcggc gctgcgggcg gtcgccgtga caccgcagcg
     1801 ccactggcgc ggtcgactcg tccgctttgc cggtgcctcg ccgcttgtcc ccgttggcct
     1861 gacgctggtc gccttcgccg ccgtcgtaac gctgggcatt ggtttcacgc aggtgctgcc
     1921 gatcccgggc accgaggcaa cgccaactcc tggtttcact gatacgccga ccccgccccc
     1981 caccaaccgg ccgtcgggat cggtcgtgta cacgaacgag gaggacggct acgagctgac
     2041 gctgccggcg cgttggaccc cggacgactt cttctccaat ccgcctggga cgatgcggtt
     2101 tggcgccgcc cacagcgacg gcgatcccag tggcttcggc gccttgaccg tgagcatcgg
     2161 cagcaccgac gggaccgtct acgactgcgc tccctccctg tgccggccta tcgttgcgac
     2221 cacgatcgac gagctggacg ccgcagtcga aaacgacagc atcgtggtcg aaacgccgat
     2281 gatcacaacg gtcgatgtcg atgtgacact ggacggcgag ccagctcgcc tggagtacgt
     2341 gaccttcgcc ggcggactga tcgagcaggg tgcgcagttc catcacctct tcgccattca
     2401 caacggacgc cccgtcgtcc tctcgttcga caagtacgcg tatggcttag ggcaagagtc
     2461 gaacgacatc ggttgggtcg aggcactcat ccagggtttc cgattcctta atcgccacga
     2521 aggcccctcc ccgaaccccg acgactccga ccagacgacg tggaagaccg tcgaactcgc
     2581 gaacgccggg tttactgttg ccattccgtc ggactgggta gtcgacgccg gtgatgaccc
     2641 ggcggtggtc acccttcgtg gcacgagtgg aagcgtccgg gtccgagctg gcgatcagag
     2701 cggtcacata tcgatgtgtc taaccctggc gccctgccag ctggtcgagg ctcgttcgat
     2761 ggacgatctc atcagggtcg ttcgcggtga gtaccgcgtc cattggctga gcgagtacaa
     2821 cctgaccgat gaaagggtaa tcgtcgacgg cgtcgacgcg gcccgtgtca ccctgagccc
     2881 ccggggaggc attagcggcc ccggcacgtc gcgatacctc gtcttcgtcc gggatgagcg
     2941 agctgtgatc ctggagtgga ggccaacctt ctctgggacg gggctgtgga acgagatcat
     3001 ctcaggtctc cgcttcatcg actgagcccg cggaaccgag cgcggcgcgg ataatcgaac
     3061 cgatccgcag tgccccgcat cgtaccgact gagcgggcct gcccaagagg gccgcacgag
     3121 gtactgacgt ggagcggagg ctcgtggagc tggcccagcg gggagaccag aaggcgttct
     3181 ccgagatcgc cttctccgtc agcccgcgcc tgttccccgt cgcccagcgc atcctccgcg
     3241 actaccagcg ggccgaggat gccacgcagc agaccctcgt ccagatctgg cgcaagctcc
     3301 ccaatctgtc ggaccccgat aagttcgacg gttgggcgta tcggatcctg gtcaacactt
     3361 gttacgccga agcccgaaag aacaagcggc atcccgacga cctgcgcctc ctcgagacgg
     3421 atcgcgccag cgccgaccat gcgctcagcg tcgcggaccg cgacatgctg gaacgcgcat
     3481 tcgatcgcct gccagccgag cagcgggccg tgctcgtcct ccagtactac ctcgacctgg
     3541 gccacccgca gatcgcggac atgctgggcg tgccactggg aacggtgaag tcgcgtgcct
     3601 cgagcgggcg ccttgcgctc cgagccgcac tggaagccga cgcacgggcc ggtattggtg
     3661 gagtggcacg atgaccgacc gcggacgacc gatgaccgag tccgagctcg atcggctgct
     3721 tggtacctgg ctcgccgagg gtgccgcagc cacgccagat cgcatcgccg agcacgcgat
     3781 gctggagatc gccacgacgc cacaggaagg aacgcggtgg tcgttgttct gggcgtcgat
     3841 gaccagcgcg cccgtggcct ggtctgcggt cgccatcctc gcggtcgccg tcggcctggc
     3901 catccttgtc ggcccgcgtt tggccgaacc caccccgacg cccaccccga gtccgagcgc
     3961 cagcccgacg gcaagtcctg gcgccaccgc cacgcaccaa tcggcgaccg ctgtcggtcg
     4021 aatcgactgg gtccgaatcg agtccgagcg ccgagtcttt ccgacgagtc cgtatggggg
     4081 tcagatcctg ggctgggagc ctaccgagga tttcgaagtc gccggcgtat acctgtcaat
     4141 ggatggcaca aactggcagc cgaccgatgc accgccttgg ggactgtccc cggaattcct
     4201 gaaccgggat aaccccaccc aggtggcgta ctcaccggat ggatacggaa cgtccatggg
     4261 cccggactcc tcgcgccaga tcatttatcg agactggacc gacaaccccg gcgacccggt
     4321 catcttccag ctagaccgcg accggtggct cccgatcgtg ctcccatcca cggcgccgcc
     4381 cccgaccgag ggattgagga tctacggcag caggatccag ggtgcggctg ccctggacga
     4441 gtcgaactgg atcgcccccg tgatgcattt cgtcgaagtt ccgtgggcag acatcctggg
     4501 tggggatctc gcggacgcgt ggccgatgtg gaaggaggat gcccagcgcc tggagattca
     4561 cgagcccggt ggcgacccgt tcggcagcgc agggctggtg aacctgaccg ttgaactggt
     4621 cgagggagat ccgccggtca tcgagttccg tgactcggag accggggacc ttgtccacgt
     4681 ggttcccgcg accttgcctg gctggtcgcc tgaagcgctt gtcacggcac tccgtgggtg
     4741 gggcctgttg gactactcct tcgtcgtcag ccgcaacggg gaaatccgcg tcgtccgccc
     4801 accctggacc atgggcgaag actggaccga catccgggtc gtcaccgctt tcggccgcta
     4861 ttacacgatc accctgcccc tgggcgaggg ctactcgtcg acggacatcc atctctggga
     4921 gtccgaggat gggatttcgt ggaccctgct ggacaccccc cagttgtgga cggggacgct
     4981 ggagtgggcc gagatcgtcg gcggtaacga ccagctgctg atcatcccgc acgacttcga
     5041 ggggagctcg ccgatgtcgg tgtcggccga tggtcggacc tggagcgaag cggtgctcgg
     5101 tgactcaacc gcgggcctgt tctccccgac gagcaccgac ttcggctggc tggggaatgg
     5161 gttcgaccag accgccatat cggccgacgg tatgaactgg gaatggatcg acctccccga
     5221 cctgccggcc gagccgtcgg tgacctacat caatgggatc ttcttctacg gaccggaaga
     5281 cgtgggaaac ggtcggtttg ccacgtgggt gggccatctg gccgactgag ccgccgctcg
     5341 cgcaggtggt cgggaaagtg aggctatttc cgaaccgatt ggggcggtcc ggcatcgttc
     5401 tcggcatgaa ccgatcattc gatcgtcggc tgagcttcct cggcggctgt gtggtggctg
     5461 gcgttctcct cgcgctcgtg gcgcaggtcg cgatatcacc cagggaaccg acggggtcag
     5521 ggctggcccc gggcgcgctc gccggacccg cggttggagt ctcgcccata cccgtgatcg
     5581 caacggagtc tgccgacgcc gcccgagtct tcgccgaggc ggggttccag ctcgtcctcc
     5641 ccgcgggctg gtcggtgaac gaggaaggcg ctgatgcggt ggtgatctcc gggcctggcc
     5701 gcaggatgct ggtgcggatc ggcgatgcgg caggacatct accgaactca tgcctgaggg
     5761 cggatcgact tgggtttgag cagtgctcgg cggtggtggt cgatgtgctg gacgacttca
     5821 agaacacctt ctgcgatgga tgcctggcat catcccggcc tgactcgctg gccggcgaag
     5881 cggccagccg ggtccggata tcgggttatg aatacgtcgc ggcgttgcac gacgggcggc
     5941 cgtatgtcgt gcgatttcgc atgacctacg cgttccagcc cagcgctccg ggggtcccga
     6001 gctgggaatc gatcctggac accttccagt tcgtcgacgc gcacgcggga agcgtcttcc
     6061 agagggagct ccagctcgcg ggcttgtcgc tcgcccttcc ggagggctgg gaacgatgcc
     6121 acgagacaaa cgagttcgtc ttccgaccgg ccgccgcatg cgcggaagcg gcatcgcgac
     6181 cgtactgggt taattcgacg ccggagccat ccgtgacggt caggctcggg acaaccggag
     6241 agcctccgac gtattgcgtc ggagtcgact ccgcctgcca gtacccggcg agtggggagt
     6301 gccaaaccac gggtgcgtgc accgacgtcg atgtcgcgag cctctcgaat ctgtggagcg
     6361 cgctcagccg cgaggacgcg ctccgctggg ccgactccga gcagcgcgat atcgagattg
     6421 acggcgggcc gggtcgcgtc gtgcacgtga gtcgggagct ccgggagggc gaaatcacgt
     6481 acgagaagac atggctcctg acgatccgag aacgacccta cgtcttgatc ttcgccgccc
     6541 catccgggtc gatgcccgac ggatgggtgc aagacatcat ctcgagtgcc gacttcgcga
     6601 atcccccggg cggccagtcc acatgggaga cgtttaccgc cgccgaggcc ggtttcgcca
     6661 ttgacctgcc tcaggactgg gtcgaagagc cgaccaatga tccgcagctg gtttgggtgc
     6721 atggcggcgg cggaggcttg cgcgtgcggg tcggcgacga gaccggggag atcctgaact
     6781 gcgatatccc ggccggctca acgtgccagt ccgtgcatgt cgaatccgtc gaaacgcttg
     6841 tcgacctcat gacgccccca ccgggcacga ccttctacgg tccggggacg gcgaacatga
     6901 accgtcgccc cgtcttgctc ggatcaatac cggcggttca agtgcgaatc gcgacgtggg
     6961 cggtcggccc atcggctcat ccgacgacgc ggtactacgt ggtcgccgtg gtcggcggcc
     7021 gtccggtcat catcaaatgg gagcctgggt cgctccacgg tgtgggattc cagcgcctgc
     7081 tggaggggtt ccgcctcttg aactgacgtg aagcgatgtg gaccggcgct gagttaggcg
     7141 gggacgccgt cgtcctcgaa caggcggtcg atctgggccg gccagttctc ctgcacgacc
     7201 cggagcttgg tcttcaacgt gggggtcagc tccccgttct cctcggacag ggcgcggggc
     7261 aggagcgcaa tccgccgcgg gcgctcgaag acggcgaagt cctcgagcag gtcgcgcacg
     7321 tcgccctcga tgagctggcg gacggccttc tcctcgatca gctgctcggg ctccatgcca
     7381 tcaagcccgt ttgcctccgc ccacggcttg agctcgtcaa agttgggcgc gatcagcgcg
     7441 ccggtgtacg ggtgccggtc acccaggatc accgcctggg cgatgtactt gctggcggac
     7501 agtgcggcct ccatcggtcc gggtgagacg ttcttgccgt tggccaggac gatgatgttc
     7561 ttcagccgat cggtgatctt gatccgaccg atgggatcca gctccccgac gtccccggtg
     7621 tggaaccagc cgtcggggtc gatggccttc gctgtctcgt cgggctggtt caggtagccg
     7681 agcatgatct gcggcccgcg gacctggatc tcgcccgtct cggggtcgat ccggacctcg
     7741 gtcgcaggcg cgggtcggcc gaccgtcccg aacacgaagt cgtcgggccg gttgatggac
     7801 acgagcgggc tcgtctcgct cagcccatag ccctcgagga tgaggatgcc cagcgcatag
     7861 aagaactccc cgacctcgcg cgacaacggg gcggaaccgg acacgaagta gcggactcgt
     7921 ccgcccgtgc gcgccttgac gcggtggtag acgagcttgt ccgcgatggc gtgctggacc
     7981 ttgaggccaa ggccatccgc ccgcccggcg acgtggttct cgtagcgctt ccggccgacg
     8041 tcgatccccc agtggaagat ccgcttccgc atcggtggtg acgactcgac cgacgcgagg
     8101 acccggccgt atacccgctc gtagaagcgg ggcaccgcgg ccatgaccgt gggctggacc
     8161 tcgaccatgt tgtcggccag ccgctcgatg agcgactcgg cgtagacgac cgtggacccg
     8221 tgtgcgagcg ggatgatctc gccggtcagg cgctcgaaga tgtgtgacaa cggcagccag
     8281 gacaggaaga tgtcagcctc gtagaagtcg acgatctgga ccgccgcctc gtagttgtag
     8341 agcaggttcc cgtgacgaat cattgcgccc tttggctgcg cagtggtacc cgaggtgtgg
     8401 atgaccgtgg ccagatgctc ccggtcgatc gcgcgccagc cggccgccca agccgcccgc
     8461 tgggcctcat ccggctcacg gcccgacagc tcctccaggg tcagcgtccc tgctggcggc
     8521 ttccccgacg tgtcgaacgt gatgacgtgg cgcagggtcg ggacctggga ccggatggac
     8581 tcgaccttgt tcgcctgctg cgcgttttcg acgaacacgg ccaccgcgcc cacgttgttc
     8641 aggatgaacg ccgcctggcc ggcttcggtc tgggggtaga tggggcagga cactgcgccc
     8701 agcgcccagc tggcgatgtc cgccaccagc cactcgggac gggtgcgtga catgatgcag
     8761 accgagtcgg agtccttcac gcccaggccc ttgagcccga gagacgtccg cgtgatccgg
     8821 tcccagaact cggcgtacga gttgctccgc caaccaccgt ccttgggcag cttccagcga
     8881 agtgcctcct tgtccggaat gcgctcgacc gaccgatgaa tgagctccac cacgttccgg
     8941 gccttctcgg actcgggcag gagaagggcc tcgatctcgt tccgcatcag cttgccgccc
     9001 gcgggtgtgg cagcggggcc ggtggcagcc gacggcgtgg gcgtggcagt cgtggtcatc
     9061 ggtccttcct ctcgtccggg ggcgcggggc tgggctagcc gatcttattc ccctgctcgt
     9121 cgtaccgata gaacccgcgc ccggtcttcc gacccaggtg gccgccggcc accatctggc
     9181 ggagaagcgg cgcggcgcgg aacttcgggt cgccaaagcc acggtacagg acgtccatca
     9241 cgttgagcat cacgtccacc ccgacgaagt cggccagctc gaggggcccc atcggatggt
     9301 tcaacccgag ccgggccccg gtatcgatct cgtcggccgt gccggtccct tcggccaggg
     9361 cgaacacggc ctcgttgatg aacgggccga gcatccggtt gacgaggaag ccgggatagt
     9421 ccttggacgt gatgaccgtc ttgcccagct cgtcggcgaa cgcgcggcac gcctgctcgg
     9481 tctggtccga ggtctcgagc ccacggatga tttcgatcag cttcatgacc ggcacgggtg
     9541 agaagaaatg gagaccgatg aaccggtccg gccgatcggt ggcggtcgcc agcgcggtga
     9601 tgctgatcga cgaggtgttg gacgcgaaga tcgcgtccag gtcggcggcg tcggtgaccg
     9661 cggtccatga ctcgcgcttg atggcctcgt cctcgaagac cgcctcgatc acgatgtcgt
     9721 ggcccgcgac accatccacg ccgaccgcgg tcgagatgcg cccgagggtc gcgtcggcct
     9781 cgtcctgtgc gagggggccc ttctcgacca ggcgggcgag attgccggcg atccgctgcc
     9841 ggccggcgat ggccaggtcc tcggtccggt cggcgatggt gacctgcttg ccggccgcgg
     9901 cgctgacctg ggcgatgccg tggcccatca ggcccgcacc caggacgtag acccgctcga
     9961 ggctcatcgg ctcaagcgta ccagcgcggt cgtcgacggt cggggggtca accgagcagc
    10021 cggtcggcgg ccgcgaacgg atcctcgcgc cgctcggcga ccgccgccac ggcgtcgtcc
    10081 cagccgggtg catggagggc gcggtcgtgg gcccggctgg cgagcgcgcg acggagcagc
    10141 tcggtcgcgc gggccagatc ccgctccgga gtccgctgcc gccgatgtgc ctcgatcgcc
    10201 tcgcggagcg tttcgacgcc ctccccggtc gtggccgagg tcaggaggac cggtggcgcc
    10261 ggccgggcca cgcggccgcc ggccgtggac agcatggcct tcagctgccg cgcggcttga
    10321 tccgcgccgg gccggtcgct cttgttgacc accaccacgt ccgcgatctc gagcaggccg
    10381 gccttgatgg cctgcacctc gtcccccatc tcgggcacct cgacgacgac cgtggtctcg
    10441 gccagggtcg cgatctcgac ctcggactga cccgcgccca cggtctcgat gagcaccggg
    10501 tcgtagccgc ccgcgtcgag cgcggccgcg gtcagccagg tcgtggccgc caggccaccc
    10561 agggccccgc gcgatgccat cgagcggacg aacaccccat cgtcgccgac gtggtccatc
    10621 atccgaatcc ggtcgcccat gatcgcgccg ccggtcagcg gggagctggg gtcgacggcg
    10681 atcacgccca cccggcgtcc ctgcgcccgc caggcgcgaa tcagcgcatc gaccagcgtg
    10741 gacttgccgc tccccggcgg gccggtgatc ccgaccaggt gcgcgccatg gcccgcggcg
    10801 aacaccgcgc cacggtctga cgcgtccagg gcaccgtcct ccagatcggt caggagccgg
    10861 gccagcgcca ggcggtcccc ctcctgcgcc tcccgcagcc gttccgcgag gtcagccacg
    10921 cgggcgggcg tggtcccgga agtaggtcgc gacctggccg atggtggtcc ccggtccgaa
    10981 cacctcggcc accccggcgg ccttgagctt cgagatat
//
LOCUS       DASYGU010000177         7093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1675529, whole genome shotgun sequence.
ACCESSION   DASYGU010000177 DASYGU010000000
VERSION     DASYGU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675529"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VGO32_04920"
     CDS             complement(<1..1502)
                     /locus_tag="VGO32_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7200128.1"
                     /translation="MADTARVVIIGGGVGGTAIAYHLAHLGWTDVMLLDRSVLTSGST
                     FHSAGLVGQLRASPTLTRMMVDSVATYRQLAAETGVDPGWREVGSLRLASSDERMEEL
                     RRQAGWAKAYGLEMALVSAEGAREQFPLMSTDGVRGAAWLPTDGYLDPSGLTQALAAG
                     ARQRGVTIRPRTRVTGITVAGGRVTRVETDGGPIDCEVVVNAGGMFAPEIGRMAGVTV
                     PVIPFAHQYLVTEPVPGVSPELPQLRDPDNLVYFRPEVAGLVMGGYERNPAPWGLDGI
                     PADFNGKLLPPDWPRFEEISRGAQRRVPAMADAGVRQVINGPEGFTPDNEFILGESEV
                     RGFFVAAGFSAHGIAGAGGMGRQMAHWIVDGEPEYDLWRMDIRRFGASYRSRDYTLAR
                     STENYATYYDIHYPYEERQSGRPLHVSPTYDRLVQLGASFGEKSGWERPNWFTSNDEA
                     GDVSLRPRGWAGQHWSPAIGAEALATRQAAALYDETSFAKIEVSGPGAIAL"
     gene            complement(1495..2478)
                     /locus_tag="VGO32_04925"
     CDS             complement(1495..2478)
                     /locus_tag="VGO32_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7200129.1"
                     /translation="MSSGLARQPMEAAVPDETLHGELAEHLASALQRVPETAGRDLAF
                     TPLSGGITNRNFLITIAGDADRYVIRLAGNDTHLLGISREVEHAATVAAAGVGVGPEV
                     AAFIRPEGYLVTRFIVGSPVSMEEVRGPAMLERVADSIRRVHGGPAIPGLFVPLRVAE
                     AYLALARSRGVPEPRGWAAAHDAGHRIERALVDARLPLLPCHNDLLNANFIDDGERIR
                     IVDWEYAGMGDPFFDLGNFSINHELTPDQDAAFLAAYDQTDSAAPARLARLTLMRVVS
                     DFREAMWGVLQQAISTLDVDFVAYAEEHFDRLLRHAAEDRFEQALARVSDG"
     gene            complement(2491..3276)
                     /locus_tag="VGO32_04930"
     CDS             complement(2491..3276)
                     /locus_tag="VGO32_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200130.1"
                     /translation="MTLRPDQRANAIDRSVLEGEIARIVDGLDGEHVPLRVLGSLGVS
                     IHCPASAPLLLAFERTYADIDFAGYRRQSSVVGSYLSALGYVADQEVAITSEGRRGLW
                     DHRSNGIHLDVFFDQLDFCHQIPLNGRLEADRPTIPLAELVLSKLQIVEINEKDVIDV
                     ILLLLDHDLADSDDDAIDARRIARMCAADWGLWRTSTRNLEKVQALASTYPQLDAPHR
                     EKVESQTAALAARLDQEPKSLQWRTRARIGERVKWWTDVDEVR"
     gene            3439..5883
                     /locus_tag="VGO32_04935"
     CDS             3439..5883
                     /locus_tag="VGO32_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7200131.1"
                     /translation="MVEQARVVVIGGGITGVSVALHLARAGWREIVLLEKGVLTSGAT
                     SQAAGVVTAFNPSAAMMSFRRYSIDLYREFDVFETVGSIRIASSREQWRELQRGASRA
                     RGIGLEVELLGPRETLRLLPEASPADLHGSVWLAGDGYLDPHGATYAVAAAARALGVT
                     IETGVRVVAIELGSRHEVKAVVTDRGRIVCEEVVNAAGMWAPRVAAMVGSWLASTAVD
                     HQHIALRAVDGHALPPDMPCFRDPDNLVYGKAEQGGMLFGGYEAEPHARWLDGAPWEH
                     GSRSLPPDWVRFEPLMAGAIRRFPFLADAEAIRLVNHPDAMTPDANPLLGPMPGVHGF
                     WVAAGLSLNGFGGAGGIGRAIAGWMTADDPGVDIHPYRAWRFGATYRDPRYVAETARE
                     AYRYYYRLRYPYDSDTAGRPRRLSPLHTRLEDAGAVFGTKHGWERADYLEPSTPWRMA
                     GEDQRTYGWSRPPWLDLVGEEHRAVRERAGLIDLTSFGKIDVSGPGALGLLQRVSVAD
                     VDRPVGTVVYTQWLDDRAGMVADVTVVRLAADRFRVVTGAGYVASDLGWLLLHVREGE
                     EVALRDVSEELACLGLWGPRARDIFAAVTDDDVSSGGIPLRTARPVRVGGGDVLAQRI
                     SYAGELGWELYVEPSWAVQLWDRLMDAGVEHGLAVFGYRALDSLRMEKGYRYYGTDLT
                     MAETPLEAGLGRFVAWDKPDFVGRDALAAHRDAGPPAVRIRSVLVGPGPSDEDDDGWL
                     PVYGGEAVRFEDEPAGRLRSVGYGHSLRRVIGYVYLPTHVAEGSEVEIEILGQATSAT
                     VVPDALVDPKGDRMRG"
     gene            5898..6893
                     /locus_tag="VGO32_04940"
     CDS             5898..6893
                     /locus_tag="VGO32_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV7200132.1"
                     /translation="MPLVGGRTRGKQTKVFFATDVHGSERTWRKFLSAAKFYAADVLV
                     MGGDVMGKLAVPVIREKNGHHRATIHGRVEQLDTAAEVEQAREIIGGLGFYDVVMDED
                     EYRTVRDDPAAIDELWRRLATERLARWIELTEDRLGPAGIRCFVSGGNDDLLEVMEWL
                     PSEGTRSFTACESRVVPLDDDHVMVSLPYVNPTPWHTPREAPEEELAAMIDRNVEGIT
                     DFGNVVFNFHAPPIDSTLDTCPMLDATTDPPSQVVRGGQPVLYGAGSTAVRAALERYQ
                     PLLGLHGHIHESQAAARLGRTLCINPGSEYGEGVLRGTLVNLVNGKVDSYQMTTG"
BASE COUNT         1100 a         2342 c         2515 g         1136 t
ORIGIN      
        1 agcgcgatcg cgcccggtcc gctgacctcg atcttggcga agctggtctc gtcgtacagg
       61 gccgccgcct gccgggtcgc gagcgcttcg gctccaatcg ccggcgacca atgctgcccc
      121 gcccagccgc ggggccggag cgagacgtcg ccggcctcat cgttcgacgt gaaccagttc
      181 ggccgctccc agccggattt ctcgccgaac gacgccccca gctgcaccag tcggtcgtag
      241 gtcggcgaca cgtggagtgg ccgaccggac tgacgctcct cgtaggggta gtggatgtcg
      301 taataggtgg cgtagttctc ggtcgagcgg gccagggtgt agtcgcgcga ccggtaggac
      361 gccccgaagc ggcggatgtc catcctccac aggtcgtact ccggctcgcc gtccacgatc
      421 cagtgcgcca tctgccgtcc catcccgccc gcccccgcaa tgccgtgggc ggagaagccg
      481 gcggcgacga agaagccgcg cacctcggac tcgcccagga tgaactcgtt gtcaggggtg
      541 aaaccctccg gcccgttgat cacctgccgc acgccggcat cggccatggc cggcacccgt
      601 cgctgagcgc ctcggctgat ttcctcgaac cgtggccagt ccggcggcag gagcttgccg
      661 ttgaagtcgg ccgggatccc gtccaggccc catggcgctg gattccgttc gtagccaccc
      721 atcaccaggc cggcgacctc gggtcggaag tagacgaggt tgtccgggtc gcggagctgg
      781 ggcagttcgg gggaaacccc cggaaccggc tcggtgacga ggtattgatg ggcgaacggg
      841 atgaccggca cggtcacgcc cgccatccga ccgatctcgg gcgcgaacat cccacccgcg
      901 ttcacgacca cctcgcagtc gatcggtccg ccgtcggtct cgaccctggt cacccggccg
      961 cccgccacgg tgatcccggt gacccgggtc cgtggccgaa tggtcactcc ccgctgccgg
     1021 gcgccggcgg ccagggcctg ggtcaggccg gacgggtcga ggtagccgtc ggtcggcagc
     1081 caggcggcgc ctcggacgcc gtcggtcgac atgagcggga actgctcgcg cgcgccctcg
     1141 gccgaaacga gcgccatctc cagcccatag gccttcgccc agcccgcctg gcggcggagc
     1201 tcttccatcc gctcgtccga ggacgccagg cgcagggacc cgacctcgcg ccagcccggg
     1261 tccactccgg tctctgcagc cagctggcgg tacgtcgcca ccgagtcgac catcatccgg
     1321 gtcagggtcg ggctggctcg cagctggccc accagtcctg ctgagtggaa ggttgacccg
     1381 ctcgtcagca cggatcggtc caggagcatg acatcggtcc agcccaggtg ggcgaggtgg
     1441 taggcgatcg cggtgccgcc cacgccgcca ccgatgatga cgacccgggc ggtgtcagcc
     1501 atcgctgacg cgggccaggg cctgctcaaa gcggtcctcc gcggcatggc gcaggagccg
     1561 gtcgaagtgc tcttcggcgt aggccacgaa gtccacgtcg agggtgctga tcgcctgctg
     1621 gaggacgccc cacatcgcct cgcggaagtc ggaaacgacc cgcatcaagg tgaggcgcgc
     1681 gaggcgggcg ggcgcggccg aatcggtctg gtcgtaggcg gccaggaagg cggcatcctg
     1741 gtccggagtc agctcgtggt tgatgctgaa gttgcccagg tcgaagaacg ggtcgcccat
     1801 gccggcgtac tcccagtcca cgatccggat ccgctccccg tcgtcgatga agttcgcgtt
     1861 cagcaggtcg ttgtggcacg gcaggagcgg gagccgcgcg tccaccagtg cccgctcgat
     1921 tcggtggccg gcgtcgtgtg cggcggccca tcccctcggc tcgggcacgc cccgagagcg
     1981 ggccagggcc agatatgcct cggccacccg gagcgggacg aacaggccgg ggatcgccgg
     2041 cccgccatgg acccgccgga tcgagtccgc cactcgctcg agcattgccg gtccgcggac
     2101 ttcctccatg gacactgggc tgccaacgat gaagcgggtg accaggtagc cctcggggcg
     2161 gatgaaggcg gccacctcgg ggccaacccc aacgccggcc gcagccacgg tggccgcgtg
     2221 ctccacctcc cggctgatcc ccagcaggtg ggtgtcgttg ccggcgaggc ggatgacata
     2281 ccgatcggcg tcgccggcga tcgtgatcag gaagttgcgg ttcgtgatcc cgcccgatag
     2341 cggcgtgaaa gcgaggtcgc ggcctgccgt ttcggggacg cgctgaaggg cgctagccag
     2401 gtgctccgcc agctcaccgt gaagcgtttc atccgggaca gcggcctcca tcggttggcg
     2461 agccaggccg ctgctcaccg ccgcatccac ctagcggacc tcgtccacat cggtccacca
     2521 cttgacgcgc tcaccgatgc gtgcccgcgt tcgccactga agtgacttcg gttcctggtc
     2581 gagccgcgcg gcaagcgccg cagtctggct ctcgaccttt tcgcggtgag gcgcatccaa
     2641 ctgcggatag gtcgatgcca gcgcctggac cttttcgagg ttccgggtgc tcgttcgcca
     2701 caggccccag tccgccgcgc acatgcgagc gatgcgtcgc gcgtcgatcg cgtcgtcgtc
     2761 gctgtccgcg aggtcgtggt cgaggaggag caggatgacg tcgatcacat ccttctcgtt
     2821 gatctcgacg atctggagtt tcgagaggac cagctcggcg agggggatgg ttggtcgatc
     2881 cgcttcaagc cgaccgttga gagggatctg gtgacagaaa tccagctggt cgaaaaagac
     2941 gtcgagatgg atgccgtttg atcggtgatc ccacagaccg cgccgaccct ccgacgtgat
     3001 tgccacctcc tgatccgcca catatcccag ggccgagaga tacgacccaa ccacgctgga
     3061 ttggcgccga taccccgcga aatcgatgtc cgcataggtc ctctcgaacg caagcaggag
     3121 gggagccgac gccgggcagt ggatcgacac gccaagcgaa ccgagaaccc ggagcggaac
     3181 atgctcgccg tcgagcccat ccacgatcct ggcgatctca ccttcgagca ccgaccgatc
     3241 aatcgcattc gctctctgat cggggcgcag cgtcatcggc gatctccgcg gtggggcagg
     3301 cgggcattgt acggattgga agggcgcgtg ccgcggtcca tgacgtggcc atgcgcgggc
     3361 acggcatcat tgcagggctc cttgccagaa ccgcagtgtt ggcgctgggc aggcgcacga
     3421 acagggaccg gggagcggat ggtcgagcag gcacgcgtcg tggtgattgg tggggggatt
     3481 accggggtca gcgtcgcgct tcatctcgcc cgcgcggggt ggcgggagat cgtgctgctc
     3541 gagaagggcg ttctgaccag cggggcaacg agccaggcgg cgggcgtggt gacggcgttc
     3601 aacccctcgg ccgcaatgat gtcgttccgt cgctacagca tcgatctcta ccgtgagttc
     3661 gacgtcttcg agacggttgg cagcatccga atcgcgtcca gccgcgagca gtggcgggag
     3721 ctccagcggg gtgcgagccg agcccggggc atcggactcg aggtcgagct tctcggtccc
     3781 cgggagaccc tccggctcct acccgaagca tccccggccg acctccatgg ctcggtctgg
     3841 ctggccggag atgggtacct cgatcctcat ggggcgacct atgccgttgc cgctgccgct
     3901 cgcgcgctgg gggtgaccat cgagacgggc gttcgcgttg tggccatcga gttgggcagc
     3961 cgccacgagg tgaaggcggt ggtgaccgat cgcgggcgga tcgtgtgcga ggaggtggtg
     4021 aatgccgcgg gcatgtgggc gccgcgtgtt gcggccatgg tcggatcctg gctggccagt
     4081 accgcggttg accatcaaca catcgcgttg cgggcggtgg acggccatgc gttgccacca
     4141 gacatgccct gtttccgcga cccggacaat ctcgtctacg gcaaggcgga gcagggcggc
     4201 atgctgttcg gggggtacga ggcggagccg cacgcccggt ggcttgacgg cgcgccgtgg
     4261 gagcatgggt cgcgctcgct cccacccgac tgggtccgct tcgagccgct gatggccggg
     4321 gccatccggc ggttcccgtt cctggccgac gccgaggcca tccggctggt caaccacccc
     4381 gatgcgatga cgcccgacgc gaatccgctg ctcggtccca tgcccggggt ccacggcttc
     4441 tgggtcgccg cgggcctctc gttgaatggg ttcggtggcg ccggggggat cggtcgagcg
     4501 atcgccggct ggatgacggc cgacgacccc ggggtcgata tccaccccta tcgggcctgg
     4561 cgcttcgggg ccacctatcg cgatccccga tacgtggccg aaacggcccg cgaggcgtat
     4621 cgctactact accggctgcg ctatccctac gacagcgaca cggctggtcg tccccgaagg
     4681 ctcagcccgc tccatacccg gctcgaggac gccggtgcgg tcttcggaac caagcacggc
     4741 tgggagcgcg cggactacct ggagcccagc acgccatggc ggatggcggg cgaggaccag
     4801 cggacatacg gctggtcgcg tccgccctgg cttgatctgg tgggcgaaga gcatcgggcg
     4861 gtccgcgaac gggccgggct gatcgacctg acctccttcg gcaagatcga cgtcagtggg
     4921 cccggggcgc tgggcctgct gcagcgcgtg tcggtagccg atgtagaccg gccggtcggg
     4981 acggtcgtct acacccagtg gctcgatgac cgggccggca tggtcgccga cgtcacagtc
     5041 gtccggctcg cggccgaccg gttccgagtt gtgaccggtg cgggatacgt cgcgagcgat
     5101 ctcggttggc ttctgctgca cgtccgcgaa ggtgaggagg tcgcgctgcg tgacgtcagc
     5161 gaggagctgg cctgcctggg tctgtgggga ccgcgggcgc gcgacatctt cgccgcggtc
     5221 accgacgacg acgtcagcag tggcggcatc cccctccgca ccgcccgccc cgtgcgggtc
     5281 ggcggcggcg acgtgctggc ccagcgcatc tcgtatgcgg gggagctggg atgggagctg
     5341 tacgtggagc catcgtgggc cgtgcagctg tgggaccgat tgatggatgc tggcgtcgaa
     5401 cacgggctcg ccgtcttcgg ttaccgcgcc ttggacagcc tgcggatgga gaagggatat
     5461 cggtactacg ggacggatct gacgatggcg gagacgcccc tcgaggccgg cctcggccgg
     5521 ttcgtggcct gggacaagcc cgacttcgtg ggccgcgatg cgcttgccgc ccaccgggac
     5581 gccgggcctc cggccgtacg gatccggagc gtcctggtgg gccccgggcc ttcagacgag
     5641 gacgatgacg gctggttgcc ggtgtacggc ggcgaggcgg ttcggttcga ggatgagccc
     5701 gccggccggc tgcggagtgt ggggtacgga cactcattgc gccgcgtgat cgggtacgtg
     5761 tatcttccga cccacgtcgc ggaagggtcg gaagtcgaga tcgagatcct ggggcaggcg
     5821 acttctgcga cggtcgttcc cgacgcgctg gtcgatccca agggtgatcg gatgcgcggc
     5881 tagctgggag tcaaacggtg cccctcgttg gcggacggac tcgcgggaaa cagacgaagg
     5941 tcttcttcgc caccgatgtc cacggctcgg agcggacctg gcgaaagttc ctgagcgcgg
     6001 cgaagttcta cgcggcggac gtcctggtca tgggcggcga cgtcatgggc aagctcgcgg
     6061 tcccggtgat ccgcgagaag aacggccatc accgagccac gatccacggg cgcgtcgaac
     6121 agctcgatac tgccgcagaa gtcgagcagg cgcgcgagat catcggtgga ttgggcttct
     6181 atgacgtggt catggacgag gacgagtacc ggaccgttcg agacgacccc gcggccatcg
     6241 acgagctgtg gcgacgcctt gcaacggagc gccttgcgcg ctggatcgag ctgaccgagg
     6301 atcggcttgg gccggccggc atccggtgct tcgtgtccgg cggcaacgat gacctgctcg
     6361 aggtcatgga gtggctgccg tccgagggaa cccgctcctt cacggcctgc gagagtcggg
     6421 tggtcccgct cgacgacgac cacgtcatgg tcagcctgcc gtacgtcaat ccgaccccct
     6481 ggcacacccc ccgcgaggcg ccagaggaag agctggcggc catgatcgat cgcaacgtgg
     6541 aaggcatcac cgattttggc aatgtcgtct tcaacttcca cgctccgccg atcgactcaa
     6601 cgctggacac ctgcccgatg ctcgacgcca cgaccgaccc gccttcccag gtcgtccgcg
     6661 gcgggcagcc ggtcctgtac ggcgcgggaa gcacggccgt tcgtgctgcc ctcgagcgct
     6721 accagccttt gctcggattg cacggccata ttcatgagtc gcaggccgct gcgcggctgg
     6781 gtcggacctt gtgcatcaac cccggaagcg aatacggtga aggggtgctg cggggcacac
     6841 tggtgaacct ggtgaacggc aaggtggaca gctatcagat gacgaccggt tgagggggcg
     6901 ccccgccggt agcggggtca atactgtcga gaggaggtgc ccgaggagga gccctgacgg
     6961 ccggccggcc gccgatcaaa ggcgcgagat gcgctgacta ctgatcgaac aaaggaggtt
     7021 gatagggtgg cgacagcgac cacgcttcct ggcgccccaa caccggaagt cttcgtccgg
     7081 aagtcctccg gcc
//
LOCUS       DASYGU010000178         9387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_698040, whole genome shotgun sequence.
ACCESSION   DASYGU010000178 DASYGU010000000
VERSION     DASYGU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698040"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1147
                     /locus_tag="VGO32_04945"
     CDS             218..1147
                     /locus_tag="VGO32_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200133.1"
                     /translation="MTVLTRNDPAIVWLLEGDPAIRWQVMRDLLDEPVEVWTAEQRRT
                     VEIGWVAQILAIRAADGSWPKGRWTDAPWTPLLLMALGLPEEHPAPWAQRDGLLDRLM
                     PPGRPVDRKVLLTRMDLCHLGFWLGLGSTYLSGDPRLGPLAETVISVQLADGGWNCHI
                     RNRPSTTHSSFNTTMNILENLRIAYEHGVIDDRAFREAEAGAIEFLLAHRLYRSHTTG
                     EVADHRFVQLTYPWHWHYNLMRGLDYLRLTPALIEERASDALDLLRDARRPNGRWPLQ
                     SRIPGTLLVEMEKPGGESRWNTLRALRILRAVE"
     gene            1160..1729
                     /locus_tag="VGO32_04950"
     CDS             1160..1729
                     /locus_tag="VGO32_04950"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV7200134.1"
                     /translation="MKIYTRKGDTGDTGLLFGGARVAKDSPRIEAAGSVDEAVAALGM
                     ARAALGAITDRKEARLSETIIRLQRELFVVGAEIATHKDKRAKLTDGVSRVTPDMVTA
                     LEAVIDEWTARDPMPAEFIIPGESEPGAALDLARSITRRAERRVVALAEAGDLPDSEV
                     LRYLNRLADLMFVMARAADGGFRPVRTGA"
     gene            1828..3192
                     /locus_tag="VGO32_04955"
     CDS             1828..3192
                     /locus_tag="VGO32_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV7200135.1"
                     /translation="MGYAVTNPATGETVKTYDTITDAELEAAIAAADEAHRTWSKSTT
                     VEVRAALIHRVSALHRERRQELAELIVREMGKPIGQALAEVDFAADIYEYYADHSPDF
                     LKDEPIELLAGDGSAVIRRSSLGVLLGVMPWNFPYYQVARFAGPNLIIGNTILLKHAA
                     QCPESAAAMEQVFLDAGFPAGAYVNLYASHDQLERVIADPRVQGVSLTGSERAGAAVA
                     EIAGRHLKKVVLELGGSDPFILLSTDDLDAAAQNAVDARLDNSGQSCNAAKRFIVADE
                     LYEPFLEKFTAKIAAIEATDPTSAESALGPLSSLKAAEGLDAQVKRAIDHGATLVHGG
                     SRDGAFFHTTVLTDITPENPASREEFFGPVAQVYRAKDEADAVRIANDTPFGLGSYVY
                     TTDPEQALRVADGIEAGMVFVNVVLADGAELPFGGIKRSGSGRELGRFGADEFVNKKM
                     IRIG"
     gene            3203..3643
                     /locus_tag="VGO32_04960"
     CDS             3203..3643
                     /locus_tag="VGO32_04960"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7200136.1"
                     /translation="MASLTRSVTIDRPIEDVFAVVTDVTKTGQWFPGDVVETWTTPPP
                     HGVGSVRHAVVMVMGRRSENDATVIEFDPPHHGVMAGEQQGVKWTATVACAEVEGGTR
                     LDMFFEAKAGGAMRLFLGPFATWYGNSWTTGLANLKRMMESGEL"
     gene            complement(3640..4767)
                     /locus_tag="VGO32_04965"
     CDS             complement(3640..4767)
                     /locus_tag="VGO32_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7200137.1"
                     /translation="MSDPAHRRRHRHHPYGRQRPPWWPEGEAWPPADAERTEAWQHAR
                     GGMIWRFGCFLVVLIVLIFGFVALLDWLIGGAMGGSPVAAIVLLAFVVLVALGIGSRA
                     FRRVAVPVADLVEASGRVEAGDFSARVAEDGPREVRSLARAFNTMSARLEEIEQQRQS
                     TLADVSHELRTPLAVIQGNVEAVLDGVYPADAEHLEPILEATRVMERLIEDLRTLSLA
                     EAHRLVLHREPTDLDALFTDVAAGYRSKAQQGGVQLIVSVAEAVPVMDVDPARLREVV
                     ANLLANALRHTPSGGSVDLAARVTGEVVEVTVRDTGSGMTPEELGQIFDRFYRSPDSP
                     GSGLGLSIARDLVEAHGGTITATSAPAQGTTVRFTLPLSGS"
     gene            complement(4760..5437)
                     /locus_tag="VGO32_04970"
     CDS             complement(4760..5437)
                     /locus_tag="VGO32_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7200138.1"
                     /translation="MARVLVVDDEPRIVQLVRDYLERAELEVFVARSGPEALMRARQD
                     RPDLIVLDLGLPGLDGLEVTRRLRRDSGVPIIMLTARDDETDKIVGLELGADDYVTKP
                     FSPRELTARVRAVLRREARDTEAELIRVNELVLDVPRLRAEVAGRTVPLTATEFQLLT
                     ALARQPGRVFTRSQLLDAIHGVAFASYERAIDAHIKNIRRKLEPDPRRPRYLLTVYGV
                     GYRLADV"
     gene            complement(5502..5987)
                     /locus_tag="VGO32_04975"
     CDS             complement(5502..5987)
                     /locus_tag="VGO32_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200139.1"
                     /translation="MYGFFRFVAFLLAVGLLAVVGISIYNAGVSAGIAADAGVLPPFV
                     GQPWGTGWDGGFFGFVFGLFFLFLFFGLIFGLFRAAFGWGHGGRHHGWGGHHGYRGYG
                     HGYRGYGPADMPDQEKGPDQRREPRDAFEAWHRELHDEESGTPDTPVGSAKPEPPATS
                     T"
     gene            6117..8225
                     /locus_tag="VGO32_04980"
     CDS             6117..8225
                     /locus_tag="VGO32_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200140.1"
                     /translation="MRDRTLEGLALVGAAAFLLGIRMLDGTDLASSWSGAWEGMTSQL
                     PVTFPAAVLVLLTTAIQLTAGSVVVRALRGRPYRGLIDGALGGLVGAVVIGLVTLMLL
                     GSLGLFTVPVLLLINGVVIGLGWFVRPVFEGRQELRLGWPTAAGALVVLAWFGSVFLQ
                     LASPVVPFIDVLPNHVAPAQHLATFGAFDVLTTAPSPIYGPSRMFLGYTGLLGTATVL
                     SGQPAGLAVSAFILPATVLVGAGMVRLGSGIHGVGVGWWMLLVFTLTESFARMADARA
                     TVIVLALTAFCLVELLEGDERARPLTLAVALAAAIFLHPLVGVLTTAVVVTMVAIYPD
                     RYARLGVPALVGGGILALPQATTMVGVDLPSVLGLFVIPPAIGAVWLFDRWEAGRRWF
                     ALGLRVVAVIAVVAVLILAWPQAGPLIQSFSDFFELYPVLSLTVVIAGAIAGRRAFAP
                     VAVAAFFMGLLAVVAAAAIPWQTIGIEGLDFEVAKTLHYWTPVFMAVIAAYALRAVWG
                     RPDFGGLLRIGMVGAFLLFIALPLRDAPVEPLHLGEHRTSETVSVDLRFAQAGFWVGY
                     PDSRYVIDPIQQELVDRFRDEVNAGLLDSTTPVLHVAFNFQQWDATPIGVFGGMIETM
                     VSEQTEVSSHTAGGRLHPFTDLDAMLVEDFPYLLLEPGGLPDDTRDRVLAAGYEPIFA
                     NAKGEIFVRSGEVARNGGSE"
     gene            8238..8735
                     /locus_tag="VGO32_04985"
     CDS             8238..8735
                     /locus_tag="VGO32_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV7200141.1"
                     /translation="MTDEELDAYRVLQVTPTAEDFVLEAAYRALARHFHPDGSAPDTP
                     RMAEINHAYDLVRTSERRKRYDRLHRTRPMGPGVAGESPSMAAQAFGGRVPPPTAGPS
                     GARPNDLSMTVDFGRYHGWTLKDLAKQDPDYLRWLSRHSSGVRFRNQIMALLPPEPLE
                     TPARR"
     gene            8829..9320
                     /locus_tag="VGO32_04990"
     CDS             8829..9320
                     /locus_tag="VGO32_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV7200142.1"
                     /translation="MEQEFDAYNLLQIDPLAEGIVLDAAYRALARRYHPDGESPNPAR
                     MADINRAYAMIRTPELRRRYDVERRLHPVGPAPAAPSPTSDLRSRLHPEETHGAAAAP
                     NEQYGPGSTLDFGRYEGWRLRDLARRDPDYLRWLVRHSSGIRYRAAIEQVLREMRVAA
                     ARG"
BASE COUNT         1416 a         3285 c         3199 g         1487 t
ORIGIN      
        1 gagatcggta ccgatggctc gccgaccgag atcaagatgg tcctgaccac gccgttctgc
       61 ccgtgggccg gcgacctcat ccagaccgtg aagcagaaga ccgaggacgt ggtcggaccg
      121 cccgtgaagg tcaccctgct cgccgaccgc tgggagcccc cacccggcct cttctaggcc
      181 tcgtcggcat tcccaccggc gctagcatcg cgccgaaatg acggtcctca cccggaacga
      241 ccccgcaatc gtctggttgc tcgagggcga ccctgccatt cgatggcaag tgatgcgcga
      301 tcttctggac gaaccggtgg aggtgtggac agccgagcag cgccgcacgg tcgagatcgg
      361 ctgggtcgcg cagatcctcg ccatccgggc ggctgacggc agctggccga aggggcgctg
      421 gaccgacgcc ccgtggacgc cgctgctgct gatggcgctc gggttgcccg aggagcaccc
      481 ggccccctgg gctcagcggg acgggttgtt ggaccgcctc atgccgcccg gtcggcccgt
      541 cgaccgcaag gtgctgctca cgcggatgga cctgtgccat ctggggttct ggcttggcct
      601 tggatcaacc tatctctcgg gcgacccgcg cctgggtcca ctggccgaaa cggtcatcag
      661 cgtccagctc gctgatggcg ggtggaactg ccatattcgg aatcgccctt ccacgaccca
      721 cagctcgttc aacaccacga tgaacatcct cgagaacctg cggatcgcgt acgagcatgg
      781 ggtgatcgac gaccgggctt ttcgcgaggc cgaggctggc gcgatcgagt tcctgctcgc
      841 ccatcgactc taccgctcgc acaccaccgg cgaggtggcc gaccatcggt tcgttcagct
      901 cacgtatccg tggcactggc attacaacct catgcgtggc ttggactatc tccgcctcac
      961 gcctgccctg atcgaggagc gagcgtcgga cgccctcgac ctgcttcgtg atgctcgccg
     1021 gcccaacggc cgctggccgc tccagtcccg gatccccggc acgctgctgg tggaaatgga
     1081 gaagcccggc ggggagagcc ggtggaacac gctgcgcgcc ctccggattc tccgagcagt
     1141 ggaatagcat cggccggaca tgaagatcta tacccgtaag ggcgacaccg gtgacactgg
     1201 gctcctgttc ggcggcgccc gggtggccaa ggacagcccg cggatcgagg ctgcagggtc
     1261 ggtggacgag gcagttgccg cgctcggaat ggcgcgcgcg gcgctggggg cgatcaccga
     1321 ccgcaaggag gcgcgcctga gcgagacgat catccgactc cagcgcgagc tgttcgtggt
     1381 cggggccgag atcgcgaccc acaaggacaa gcgcgccaaa ctgaccgacg gggtcagccg
     1441 cgtgacccct gacatggtca ccgctctcga ggccgtgatc gatgaatgga ccgcccgcga
     1501 tccgatgccc gccgagttca tcatccctgg cgagtcagag ccgggggcgg ccctggacct
     1561 ggcccgctcg atcacccgtc gcgccgagcg ccgagtggtg gccctggccg aggccggcga
     1621 cctcccggac tccgaggtcc tccgctacct gaatcgcctc gccgacctga tgttcgtgat
     1681 ggcgcgggcc gctgacgggg gcttccgtcc ggtccgaacc ggagcgtaga atcggctccc
     1741 caactcgatt catcgtcttc gctgacggac gagtgactgc gcgcctgacc ggctctggcg
     1801 tggcgcactg acaaggagac ccacacgatg ggctatgccg tgacaaaccc cgccaccggc
     1861 gagacggtca agacttacga caccatcacc gacgccgaac tcgaggccgc gattgccgca
     1921 gccgacgagg cgcaccgcac ctggtcgaag tcgaccacgg tcgaggtccg cgcggccctc
     1981 atccaccgcg tcagcgcgct ccatcgcgag cgccgccagg agctggcgga gctcatcgtg
     2041 cgcgagatgg gcaagccgat cgggcaggcg ctggccgagg ttgacttcgc tgcggacatt
     2101 tatgagtact acgccgatca ctcgcccgac ttcctgaagg acgagccgat cgagctcctt
     2161 gcgggcgatg gctccgcggt catccgccgc tcgtcgctcg gggtgctgct cggcgtcatg
     2221 ccgtggaact tcccgtacta ccaggtcgcc cgcttcgccg gtccgaacct gatcatcggg
     2281 aacacgatcc tgctcaagca cgccgcacag tgccccgagt ccgccgccgc gatggagcag
     2341 gtgttcctcg acgcgggctt tccggctggc gcctacgtga acctctacgc ctcacacgac
     2401 cagctcgagc gggtcatcgc cgacccgcgc gtgcagggcg tctcgctcac cggctcggag
     2461 cgcgccggcg ccgcggtggc cgagatcgcc ggccgccacc tgaagaaggt cgtgctcgag
     2521 ctcggcggct cggacccgtt catcctgctt tcaaccgacg acctcgacgc cgccgcccag
     2581 aacgcggtcg acgcccgcct cgacaacagc ggtcagtcct gcaacgcggc caagcgcttc
     2641 atcgtcgccg acgagctgta cgagcctttc ctcgagaagt tcaccgcgaa gatcgccgcg
     2701 atcgaggcga ccgatccgac gtcggccgaa tccgcgctcg gtccgctgtc ctcgctgaag
     2761 gccgctgaag gcctcgacgc gcaggtcaag cgcgcgatcg accacggcgc gacgctcgtc
     2821 cacggcggct cccgcgatgg cgcgttcttc cacacgaccg tgctgaccga tatcactccc
     2881 gagaaccccg cctccaggga ggagttcttc ggcccagtcg cgcaggtgta ccgcgcgaag
     2941 gacgaggcgg acgccgtgcg gatcgcgaac gacaccccgt tcggcctggg ctcctacgtc
     3001 tacaccaccg acccggagca ggcgctccgg gtggccgacg gcatcgaggc cggcatggtg
     3061 ttcgtgaacg tcgtactcgc cgacggcgcc gagctgccgt tcggcggcat caagcgctcg
     3121 ggctctggcc gcgaactcgg ccgctttggc gccgacgagt tcgtgaacaa gaagatgatc
     3181 cgcatcggct gatgccctag ccgtggccag cctgacgcga tcggtgacga ttgaccgacc
     3241 aatcgaggac gtcttcgcgg tcgtgaccga tgtgaccaag accggtcagt ggttccccgg
     3301 cgacgtcgtc gagacctgga ccacgccgcc gccccacggc gtcggcagcg tccgtcatgc
     3361 cgtcgttatg gtcatgggcc gacggtcaga gaacgatgcg acagtcatcg agttcgaccc
     3421 accccaccac ggcgtgatgg ccggggagca gcagggcgtc aagtggacgg cgaccgtcgc
     3481 ctgcgccgag gttgaaggcg gaacccggct ggacatgttc ttcgaggcca aggccggcgg
     3541 ggcgatgcgc ctattcctgg gtccgttcgc gacgtggtat ggcaactcct ggacgacggg
     3601 gctcgccaat ctgaaacgga tgatggagtc gggcgagctc taactcccgc tcagaggcag
     3661 ggtgaagcgg acggtcgtgc cctgcgcggg tgccgaggtg gcggtgatcg tgccgccatg
     3721 ggcctcaacc aggtcgcggg cgatggacag gcccaggccg ctccccgggg agtcgggcga
     3781 gcgatagaag cggtcgaaga tctggccgag ctcctcgggc gtcatcccgc tcccggtgtc
     3841 gcggaccgtg acttcgacca cctcgccggt cacgcgcgcc gcgaggtcca cgctgccacc
     3901 cgaaggcgtg tggcggagcg cgttggccaa caggttggcg accacctcgc ggagccgagc
     3961 cgggtccacg tccatcaccg gcaccgcctc ggcgaccgag acgatcaact gaacgccgcc
     4021 ctgctgcgcc ttgctccggt agccggccgc gacatcggtg aacagcgcat cgaggtcagt
     4081 cggctctcgg tgaaggacca gccgatgggc ctcggcaaga gagagcgtgc gcaggtcctc
     4141 gatcagccgc tccatgaccc gggtcgcctc gaggatgggc tcgagatgct ccgcgtcggc
     4201 ggggtagacc ccgtcgagca ccgcctcgac gttgccctgg atgacggcga gcggggtgcg
     4261 cagctcatgc gacacgtcgg cgagggtcga ctgtcgctgc tgctcgatct cctccagccg
     4321 cgcgctcatc gtgttgaagg cacgcgcgag ggagcgcacc tcgcgcggtc cgtcttccgc
     4381 gacacgggcg ctgaagtcac ctgcttccac ccggcccgag gcttcgacca gatcggcgac
     4441 cgggacggcc acgcgccgga aggcacgact gccgatgccg agggcgacca ggaccacgaa
     4501 ggcgagcaga acgatcgcgg cgaccggcga gcctcccatg gccccgccga tcaaccagtc
     4561 gagcagcgcg acgaacccga agatgaggac gatgaggacg acgaggaagc agccgaagcg
     4621 ccagatcatg ccgccccgag cgtgctgcca ggcttccgtc cgctcggcgt ccgccggcgg
     4681 ccacgcctcg ccctcgggcc accacggcgg tcgctgccgg ccgtaggggt ggtgccggtg
     4741 ccgacgccgg tgcgccggat cagacatcgg ccagccggta tccaaccccg tacaccgtga
     4801 gcaggtagcg cggcctccgg ggatcgggct cgagcttgcg gcggatgttc ttgatgtgcg
     4861 cgtcgatcgc ccgctcgtac gacgcgaagg ccaccccgtg gatggcgtcc aggagctggg
     4921 accgggtgaa cacgcggccc ggctggcggg cgagggcggt cagcagctgg aactcggtcg
     4981 ctgtgagcgg cacggtccga ccggcgacct cggcccgcag ccgggggacg tcgagcacga
     5041 gctcgttgac ccggatcagc tccgcctcgg tatcgcgtgc ctctcggcgg agaaccgcgc
     5101 ggacgcgtgc ggtgagctcg cgcgggctga atggtttggt gacgtagtca tccgcgccga
     5161 gctccaggcc gacgatcttg tcggtctcgt cgtcgcgagc ggtgagcatg atgatcggga
     5221 cgcccgagtc acgacgcagg cggcgcgtga cctcgagccc gtcgaggccc ggcagcccaa
     5281 ggtccagcac gatcaggtcg ggccgatcct ggcgggcgcg catgagcgcc tcgggccccg
     5341 agcgcgcgac aaaaacttcg agctcggccc gctccaggta gtcgcggacg agctggacga
     5401 tcctgggctc gtcatcgacg acgaggaccc gtgccatggt cgtcatggta gtcgggcggg
     5461 cggcctcagc cgcccgcccg catcggtctg tccgcagcga atcaggtgga cgtggctggc
     5521 ggctcaggct tggccgagcc caccggggta tcgggcgtgc ccgactcttc gtcgtgcagc
     5581 tcgcggtgcc aggcctcgaa tgcgtcgcgc ggctctcggc gttgatccgg tcccttctcc
     5641 tgatccggca tgtcggcggg gccatacccg cggtacccgt ggccgtagcc ccggtagccg
     5701 tggtggccgc cccagccgtg gtggcggccg ccgtggcccc acccgaaggc tgcccggaac
     5761 agtccgaaga tcagcccaaa gaacaaaaag aggaagaaca ggccgaagac gaacccgaag
     5821 aatccgccgt cccaacccgt gccccacggc tgcccgacga atggcggcag gactcctgcg
     5881 tcggctgcga tgccggcgga gaccccggcg ttgtagatgc tgattccgac gaccgccagc
     5941 aggccgacgg ccagcagaaa ggcgacgaag cggaagaacc cgtacatgga tcacgtaact
     6001 cctcgtttcg gaccgatgga tccggtccac tccgaggcag cctgattcct cgatatgggc
     6061 gaaatgtgaa cgtgggaggg aggaattgcg cgagtgaccc tgtaccgtac ggcgccgtga
     6121 gagatcgcac gctcgaggga ttggccctgg tcggggcggc cgccttcctg ctcggcatcc
     6181 ggatgctgga cgggacggat ctggcgtcca gctggtcggg ggcgtgggag gggatgacga
     6241 gccagctgcc cgtgacattc ccggcggcgg tgctcgtgct gctgaccacc gcgatccagc
     6301 tgacggctgg atcggtcgtc gtccgcgcgc tgcgtggtcg cccgtaccgc ggcctgatcg
     6361 atggcgccct cggcgggctg gtcggggccg tggtcatcgg ccttgtgacg ctcatgctgc
     6421 tgggcagcct cggcctgttc accgtgccgg tgctgctgct catcaatggc gtggtcatcg
     6481 gcctgggctg gttcgtccgg cccgtgttcg aggggcggca ggagctccgc ctcggctggc
     6541 ccaccgcggc cggcgcgctg gtggtgctgg cgtggttcgg ttcggtgttc ctccagctcg
     6601 cctcaccagt ggtcccgttc atcgacgtcc tgcccaacca cgtggcgccc gcacagcacc
     6661 ttgccacctt cggggcgttc gacgtcctga ccaccgcgcc gtcgcccatc tatgggccgt
     6721 cgcgcatgtt cctgggctac accgggctcc tgggcacggc caccgttctc agtggccaac
     6781 cggcgggcct ggccgtgtcc gcgttcatcc tccccgccac cgtcctggtc ggggcaggca
     6841 tggtccgcct cgggtcgggc attcacggcg tgggcgtggg ctggtggatg ctcctcgtct
     6901 tcaccctgac cgagtcgttc gcccggatgg ccgatgcccg ggccacggtc atcgtcctgg
     6961 ccctcaccgc gttctgcctg gtcgagctgc tggaagggga cgagcgcgcg cggccgctca
     7021 ccctggccgt cgccctggcc gcggcgatct tcctccaccc gctggtcggc gtgctgacca
     7081 ccgcggtggt cgtgaccatg gtcgcgatct acccggaccg atacgcgcgg ctgggggtgc
     7141 cggctctggt gggcggcgga atcctcgccc tgccccaggc gacgaccatg gtcggagtcg
     7201 acctgccgtc ggtcctgggc ctgttcgtca tcccgcccgc gatcggcgcc gtgtggctgt
     7261 tcgaccgctg ggaagccggc cgccgatggt tcgcgctcgg cctgcgggtt gtggcggtca
     7321 tcgcggtggt cgccgtcctg atccttgcct ggccgcaggc cggaccactg atccagtcgt
     7381 tcagcgactt cttcgagctg tatccggtcc tgagcctgac cgtggtcatc gccggtgcga
     7441 tcgccggccg gcgggcgttc gccccggtcg ccgtggcggc gttcttcatg ggcctgctgg
     7501 cggtggtggc cgcggccgcc atcccatggc agacgatcgg gatcgagggc ctggacttcg
     7561 aggtggccaa gacgctccac tactggacgc cggtgttcat ggccgtcatc gccgcctacg
     7621 cgctgcgggc ggtctggggt cggcccgact tcggcgggct gctgcgcatc ggcatggtcg
     7681 gcgcgttcct gctcttcatt gcccttccgc tacgcgatgc gccggtcgag ccactccacc
     7741 tgggcgagca tcggaccagc gagaccgtgt ccgtcgacct gcgcttcgcg caggccggct
     7801 tctgggtcgg ctaccccgac tcgcgctacg tgatcgaccc catccaacag gagctggtgg
     7861 accgattccg ggacgaggtg aacgccggac tgctcgactc cacgacgccg gtcctccatg
     7921 tcgcgttcaa cttccagcag tgggacgcga ctccgatcgg ggtgttcggc ggcatgatcg
     7981 agaccatggt ctccgagcag accgaggtca gcagccacac ggcgggtgga cgcctccatc
     8041 cgttcaccga cctcgatgcg atgctggtcg aggactttcc gtacctgctc ctcgagccgg
     8101 gcggcctccc cgacgacacc cgcgaccgag tcctggccgc gggctatgag ccgatcttcg
     8161 ccaatgccaa gggcgagatc tttgtgcggt ccggtgaggt ggcgcggaac ggcggaagcg
     8221 aataggatcg cgtcccggtg accgacgagg aattggacgc ctaccgcgtc ctgcaagtca
     8281 ctcccaccgc cgaggatttt gtgctcgagg cggcgtatcg cgccctcgcg cgccacttcc
     8341 acccagacgg ctcggctccc gacaccccgc gaatggccga gatcaaccac gcctacgacc
     8401 tggtccgcac gtcggagcgc cgcaagcgct atgaccggct gcaccggact cgacccatgg
     8461 gaccaggcgt agccggggag tcgccgagca tggcggccca ggcattcggc gggcgcgtcc
     8521 cgccaccgac ggcaggcccg agtggtgccc gacccaacga cctgtcgatg accgtcgact
     8581 tcgggcggta ccatggctgg accctgaagg acctcgcgaa gcaggacccg gactacctgc
     8641 gctggctgtc gcgtcactcg tcgggcgtgc ggttccggaa ccagattatg gccttgctcc
     8701 cgccggagcc gctcgagacc cccgcccgcc ggtagtaccg ggcctgccct accccataag
     8761 ggggctggca tcgaaccttg caatcctgca aggttgctcc gcagacgtaa cccctacggg
     8821 tccggggttt ggagcaagaa ttcgacgcct acaacctgtt gcagatcgac cccctcgctg
     8881 aggggatcgt tctcgatgcc gcctatcgcg cgctggctcg gcgataccac cccgacggtg
     8941 agtccccgaa tcccgcccgg atggctgaca tcaatcgcgc ctacgcgatg atccgcacgc
     9001 ctgagctccg ccggcggtac gacgtcgagc gccggttgca tccggtcggc cccgcgccgg
     9061 cggcgccgtc acccacctcg gaccttcggt cgcgcctgca tcccgaggag acccatggtg
     9121 cggccgccgc cccgaacgag cagtacggtc ccggatccac cctggacttc ggtcgctacg
     9181 agggatggcg gttgcgtgac ctcgctcgtc gcgacccgga ctacctccgc tggctcgtgc
     9241 gccactcgtc gggcattcgc taccgcgccg ccatcgagca ggtcctgcgc gagatgcggg
     9301 tcgccgccgc ccgcggctga tccggcgctt cgggcgctgt acgatcgggg ggatggctga
     9361 ctcgagcgtg gcgccgaccg agcagaa
//
LOCUS       DASYGU010000179         5220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_698126, whole genome shotgun sequence.
ACCESSION   DASYGU010000179 DASYGU010000000
VERSION     DASYGU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698126"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGO32_04995"
     CDS             complement(<1..239)
                     /locus_tag="VGO32_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200143.1"
                     /translation="MPSGPQPSVSPSGAAADAVRYVALGDSYTIGTSVTPAETWPQQM
                     VDRSGGTLILSGNLATNAFTSEDLILHQLPELDAVR"
     gene            295..1050
                     /locus_tag="VGO32_05000"
     CDS             295..1050
                     /locus_tag="VGO32_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEV7200144.1"
                     /translation="MFLSGTILNVITVLLGTGLGLLIGRRMPPRMHESLVIGLGFFTI
                     LISISLGLQLFTDPHAQPGDEVAVLAAVLAGVAIGELLRIHDGLEALGAWFQRRLVPD
                     EAEGGAGGGGSRVAEGFVTASIVFCVGPLTIVGSLQNGLTGDAQLLAVKSLLDGVAAI
                     AFAAALGWGVGLSVITILVVQGGIAALATILAPLLDEVTILAITATGGIILLGVAFRL
                     LDLRPVRVANFLPALVLAPLFVRVADLVRGWLG"
     gene            complement(1047..2090)
                     /locus_tag="VGO32_05005"
     CDS             complement(1047..2090)
                     /locus_tag="VGO32_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV7200145.1"
                     /translation="MLVEVTRGERVESRHRGTIAVADADGHLLASLGDPDDVVFLRSS
                     AKPFQLAPFVVSGRFDAYDFPDPEQTLAVMAASHAGEDRHVRLVQAALRAGGLTRSAL
                     QCGTHTPFDAETAQRLARDGEPPNELRHNCSGKHAAMALNAKAAGWDVDTYWQPDHPC
                     QVAALEVVAAVSGLRKNQIGTATDGCGVVSFALPLRAIARTFARLADPSGLTDESLRT
                     ALTRIRDAMLAYPELISGERRQFDTDLMRAVPGRLVAKGGAEGLRSVGVLAGAVDGAP
                     LGLAVKIEDGDGARRASPPAACEALRQLGVLGEVELSQLEPYAAGVVRDMPRGEAVGE
                     VRADFTLERGSRG"
     gene            complement(2098..2772)
                     /locus_tag="VGO32_05010"
     CDS             complement(2098..2772)
                     /locus_tag="VGO32_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV7200146.1"
                     /translation="MVFGKRGDAPQEELIGTLYSESPSEPATNSAPAPTGSSEHRREA
                     TEGILDSHARFNGKFVSDRDLRIDGEAQGEIECQGTLVLSPQARVRASIKAHNVVING
                     DYEGNVDSGGRFEIGSTGRVKGEIKTQVLVIKEGAFFEGSVAMTRKTPTDGTAATGSG
                     AAGESSSSSSTSAKASGGGSSAAAAKATADPEAAASIFSNEVIGEPAGAAAGGSRQPT
                     GPGDDR"
     gene            2922..3581
                     /gene="upp"
                     /locus_tag="VGO32_05015"
     CDS             2922..3581
                     /gene="upp"
                     /locus_tag="VGO32_05015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV7200147.1"
                     /translation="MPEPLPATVHPSSHPLVAHKLSLLRDRTTEPKKFRELVRELSWL
                     LGYEAMADLATEPYRLETPIEPMDGTRLGPKVGLVPVLRAGLGMVEAMLELMPMAEVW
                     HIGLYRDERTLKPVEYYNKLPDAATVQVCLILDPMLATGGSSAATVDILKAWGAIRIK
                     QVSLIAAPEGIATLSAAHPDVEIHVGAVDRQLNEKGYIMPGLGDAGDRQFGTFPPASG
                     A"
     gene            3651..3965
                     /locus_tag="VGO32_05020"
     CDS             3651..3965
                     /locus_tag="VGO32_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200148.1"
                     /translation="MCLILSALLFGPRIILLFWWLVDTARWSATFDTILLPILGFVFL
                     PWTTVMYVLVFPQGIEGLDWLVLGLALLADLATLGGGAMRGRGQMQGYQSPPAEPPMT
                     PP"
     gene            4222..4512
                     /locus_tag="VGO32_05025"
     CDS             4222..4512
                     /locus_tag="VGO32_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200149.1"
                     /translation="MCMIIILGLLGPRIAAIVWWLADSARWTLTFGDNYLLPALGIAF
                     LPWTTIVYILCFPEGIAGLDLMWLIVAVIVDMGSYLGGGVGSRNRSSFKAVQ"
     gene            4597..>5220
                     /locus_tag="VGO32_05030"
     CDS             4597..>5220
                     /locus_tag="VGO32_05030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010597934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEV7200150.1"
                     /translation="MPDQHVTVAVTGAAGQIGYALVFRIASGQMFGSQTSVELRLLEL
                     EAALPSLEGVAMELDDCAFPLLRSVVTTSSADEAFRGANWSLLVGAVPRKAGMERNDL
                     LGINAGIFGAQGRSIAAHAAEDIRILVVGNPCNTNCLIASRAAPEIPRDRWFAMTMLD
                     ETRGRALLAQRAGATVAEVRDLVIWGNHSSTQFPDAFHATIGGRAAAD"
BASE COUNT          729 a         1858 c         1770 g          863 t
ORIGIN      
        1 cgcaccgcat cgagctctgg gagctggtgg aggatgaggt cctcgctggt gaacgcattg
       61 gtcgccaggt tgccggacag gatgagggtc ccgccgctgc ggtcgaccat ctgctggggc
      121 caggtctctg ccggggtgac cgaggtgccg atcgtgtacg agtcaccgag cgcgacgtac
      181 cgcacggcat cagccgccgc cccggatgga ctgaccgatg gctgtggtcc gcttggcatg
      241 gtgcaggctc cgctcaggac gagcacgagg gtccccgccg tccacaatac ggcggtgttc
      301 ctgagcggca ccatcctcaa cgtcatcacc gtcctgctgg gcaccgggct aggtctcctg
      361 attggccggc ggatgccgcc cagaatgcac gagagcctgg tcatcggcct cggcttcttc
      421 acgatcctga tctcgatctc gctcggtctc cagctgttca ccgatcccca tgcccagccg
      481 ggtgacgagg ttgccgtcct cgccgcggtc ctggcgggcg tggccatcgg cgagctgctt
      541 cggatccatg atggcctcga ggccctcggc gcatggttcc agcggcggct cgtgccggac
      601 gaggccgagg gaggggcggg gggcggcggt tcgcgggtgg cggaggggtt cgtcaccgcc
      661 agcatcgtgt tctgcgtcgg gccattgacc atcgtcggct cgcttcagaa cggtctgacc
      721 ggcgatgccc agctgctggc cgtcaagtcg ctgctcgacg gcgtggcggc catcgcgttc
      781 gcggccgccc tgggctgggg cgtgggcctc tcggtgatca cgatcctcgt ggtccagggc
      841 ggcatcgctg ccctggcaac catcctcgca cccctcctcg acgaggtgac catcctggcc
      901 atcaccgcga ccgggggaat cattctcctg ggcgtggcgt ttcggctcct cgacctccga
      961 ccggtgcgcg tcgcgaactt cctcccagcc ctcgtcctgg ctcccctctt cgtccgggtc
     1021 gcggacctcg tgcggggctg gctgggctag ccgcgcgacc ctcgctcgag ggtgaagtcc
     1081 gcccgaacct caccgaccgc ctcgccccgc ggcatgtcgc ggacgacgcc cgcggcgtac
     1141 ggctccagct ggctgagctc gacctcgccc agcacgccca gctggcggag cgcctcgcag
     1201 gcggccggcg ggctcgcccg gcgcgcgccg tccccgtcct cgatcttgac cgccaggccc
     1261 agcggagcgc cgtccacggc cccggccagg acccccaccg agcggagtcc ctcggcaccg
     1321 cccttggcca ccagccgccc gggaacggcg cgcatgagat cggtatcgaa ctggcgccgc
     1381 tcgccagaga tcagctcggg gtaggcgagc atcgcgtccc ggatccgggt cagcgccgtg
     1441 cgcagggact catcggtcag gccggatggg tccgccaggc gagcgaacgt ccgcgcgatg
     1501 gcgcgcagcg gcagtgcgaa gctgaccacc ccgcagccat cggtcgcggt tccgatctgg
     1561 ttcttcctga gccccgagac ggccgccacg acctcgagcg ccgccacctg gcacgggtga
     1621 tcgggctgcc agtaggtgtc cacgtcccag ccggcagcct tggcgttgag ggccatggcc
     1681 gcgtgcttgc ccgagcaatt gtggcgcagc tcattgggcg gttcgccgtc gcgggccagc
     1741 cgctgggcgg tttccgcgtc gaacggagtg tgcgtcccgc attgaagggc cgaacgagtc
     1801 aggccaccag cccggagcgc ggcctgcacg aggcgcacgt ggcgatcctc accggcatgg
     1861 cttgccgcca tgaccgcgag cgtctgctcg gggtcgggga agtcataggc gtcgaagcgg
     1921 cctgacacca cgaacggggc cagctggaat ggcttggccg atgagcggag gaatacgaca
     1981 tcgtccgggt cgcccagcga ggcgaggagg tggccgtcgg catcggcgac ggcaatggtc
     2041 ccgcggtgcc gggactcgac ccgttccccc cgcgtgacct cgacgaggat gggtgggcta
     2101 gcggtcgtcg cctggcccgg tgggctgccg gctgccgccg gctgcggcgc cggcgggctc
     2161 gccgatcacc tcattcgaga agatgctggc cgcggcctcg ggatccgccg ttgctttcgc
     2221 cgcagcggcg gaggatcctc cgccgctcgc cttggccgat gtgctcgagc ttgagctcga
     2281 ctcaccggcg gcgcccgagc cggttgccgc ggtcccgtcg gtcggcgtct tgcgagtcat
     2341 cgccacggaa ccctcgaaga aggctccttc cttgatgacc agcacctggg tcttgatttc
     2401 acccttgacc cgtccggtcg agccgatctc gaagcggcca ccggagtcga cgttgccctc
     2461 gtagtccccg ttgatcacca cgttgtgcgc cttgatgctg gcccgaacgc gggcctgcgg
     2521 gctgaggacc agggtgccct ggcactcgat ctcgccctgc gcctcgccgt cgatccgcag
     2581 gtcgcggtcg ctgacgaact tgccgttgaa acgcgcgtgg gagtcgagaa tgccctcggt
     2641 ggcttcgcgg cggtgctccg atgatcccgt gggggcaggc gcactgttcg tcgccggctc
     2701 gctgggggac tcggaataca gggtaccgat gagctcctcc tggggggcgt cgccccgctt
     2761 cccaaaaacc atatgcacac ctctcgtgcg gcgggcgggc acgcgaggtc gcgcgcacgc
     2821 gatgtcaaca gggagcgata gcctatcagc ccgctgcggg tagcactagt ccgcgggtcc
     2881 gtgcccggtg cgggcacgcg ccgggcttag aatccagacc gatgcccgag cccctccccg
     2941 ccacggtcca tccgtcctcc catcccctcg tcgcccacaa gctgtcgttg ctccgcgacc
     3001 gcaccaccga gccgaagaag ttccgcgagc tcgtccgcga gttgtcctgg ctattgggct
     3061 acgaggccat ggccgacctc gccaccgagc cgtatcggct tgaaaccccg atcgagccga
     3121 tggatgggac ccggctgggt ccaaaggtcg gcctggtgcc ggtcctccgc gccgggctcg
     3181 gcatggtgga ggccatgctc gagctgatgc ccatggccga ggtctggcat atcggcctgt
     3241 accgcgacga gcgcaccctg aagccggtcg agtactacaa caagctgccc gacgcggcca
     3301 ccgtgcaggt ctgcctcatc ctggatccga tgctggcgac cggtggctcc agcgcggcca
     3361 cggtcgacat cctcaaggcg tggggcgcga tccggatcaa gcaggtgtcg ctcattgccg
     3421 caccggaggg catcgccacc ctgagcgccg cgcaccccga cgtcgagatc cacgtggggg
     3481 cggtggatcg gcagctgaac gagaagggct acatcatgcc cggcctgggc gacgcgggcg
     3541 atcgccagtt cgggaccttc ccgccggcct cgggcgccta gcccgaggcc ctttcctccg
     3601 aaccgggccc ggtggcatga tggccgcccg ctcacgaagg aagtcggcca atgtgcttga
     3661 tcctcagcgc gctcctgttc gggccgcgca tcatcctgct cttctggtgg ctcgtggaca
     3721 ccgcgcgctg gagcgccacg ttcgacacca tcctgctgcc gatcctgggc ttcgtgttcc
     3781 tgccgtggac gacggtgatg tacgtcctcg tgttcccgca gggcatcgaa gggcttgact
     3841 ggctcgtcct cgggctggcg ctgctcgccg atctcgccac gctgggaggc ggcgcgatgc
     3901 gcggccgtgg ccagatgcag ggctaccaga gcccgccggc cgaaccgccg atgaccccgc
     3961 cctaggcgga ggaagttccg ggcaacgaaa ggtcccgaag tgaccgcccc cgggccgttg
     4021 atgtgactcc gcggcgtcgg tggctgcccc ttcgggacag ttgccacagt agcgaccgag
     4081 ggcgaggtcg cgcaacgggc ttatccccga ctttgcgacg ggatgttgac gcttgtccac
     4141 accctatccc cacgattccc tgacgactac cattcacccg acacaccaaa ccactccact
     4201 caaccggagg tcctcccgcc catgtgcatg atcattattc ttggtctcct cgggcctcga
     4261 atcgcggcca tcgtctggtg gcttgcggac agcgcccgat ggaccctgac cttcggcgac
     4321 aactacctgc tccccgccct gggcatcgcc ttcctgccgt ggaccacgat cgtctacatc
     4381 ctgtgcttcc cggagggcat cgccggcctg gatctgatgt ggctgatcgt cgccgtcatc
     4441 gtggacatgg gctcgtacct cggcggaggc gtgggcagcc ggaaccgctc gagcttcaag
     4501 gcggtccagt aggcccacac gcgcggtgcc gccccgtcac ctcctgaacg agagcggcac
     4561 cagcgggcta cactcgggcc tcgtcaagga gatccgatgc ccgaccagca tgtgacggtg
     4621 gcagtgaccg gcgcggccgg ccagatcggc tacgcgctgg tcttccgcat cgcatcgggc
     4681 cagatgttcg gatcccagac cagtgtcgag ttgcgcctcc tcgagttgga ggccgccctg
     4741 ccctcgctcg aaggggtggc catggagctc gacgactgtg cgttcccgct cctgcgctcg
     4801 gtggtgacca cgtccagcgc ggacgaggca ttccgcggcg cgaactggtc gctcctggtc
     4861 ggcgcggttc cgcgcaaggc ggggatggag cgcaacgacc tgctcgggat caacgccggc
     4921 atctttggtg cccagggccg ctcgatcgcc gcccacgccg ccgaggacat tcggatcctg
     4981 gtggtcggca acccgtgcaa cacgaactgc ctgatcgcct cgcgggcggc gcccgagatc
     5041 ccgcgcgacc gatggttcgc gatgaccatg ctggacgaga cccgcggccg ggcgctcctc
     5101 gcccagcgtg ccggcgcgac ggtggccgag gtccgcgacc tcgtgatctg gggaaaccac
     5161 tcctcgaccc agttccccga cgcgttccac gccacgatcg gcgggcgcgc cgcggccgac
//
LOCUS       DASYGU010000180         6374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1520803, whole genome shotgun sequence.
ACCESSION   DASYGU010000180 DASYGU010000000
VERSION     DASYGU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520803"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VGO32_05035"
     CDS             complement(<1..869)
                     /locus_tag="VGO32_05035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7200151.1"
                     /translation="MKFGLQISSFTWPGGAEALGPTLGRVVRTADEVGFDSIWVMDHF
                     FQIRGVGPPEAPMLDGQTALGFMAAHSERARIGLMVGGIHYRAPGLWIKATTTLDVLS
                     GGRAWFGIGAAWNEEESMGLGWPFPPLGERFEMLEETLQMAHAMWSGGSGSQARHDGK
                     HYTATRLLNSPQSLSRPRVPIMIGGGGERKTLRLAAQYADASNVFGDPDTLRHKYAVL
                     RAHCERLGRPYEEIERSVLKSVDTERDSAEAIVERWGALAEAGAQHVIFSVRGVNDTS
                     RLEWLAANVFPQLR"
     gene            932..1726
                     /locus_tag="VGO32_05040"
     CDS             932..1726
                     /locus_tag="VGO32_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEV7200152.1"
                     /translation="MTISRMGSAALLAAVICGCSILPGMGGSPEVAGREFVSTSVTVD
                     GISQPLVDGTQLRIGFTADGMLMASAGCNTFAAMYRIDGGVLAINEGAVTEIGCPSDL
                     ADQDAWVFAFLGAQPLISVDGDELVLEDDNTVITLLDREVADPDLPIVGPVWVVTGLI
                     VGDAISSIPDGVVATLVFTEDGLVMVNTGCNSGSGAAEIRTGTETILFGEVALTRMAC
                     QPPASSMEAAVLRVLNADVVRYAIEASTLDLSIAGYGLQLSGAAVD"
     gene            complement(1723..2022)
                     /locus_tag="VGO32_05045"
     CDS             complement(1723..2022)
                     /locus_tag="VGO32_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200153.1"
                     /translation="MVTRTASPATSSGLCIVDLVLFFPDEGTGTAAVTAGGTTILTIN
                     MLNYYTTGSPGAGSGERIVTLSPAVAVGSGTTLALNLSACGGCERLTVTVEGASR"
     gene            2054..2314
                     /locus_tag="VGO32_05050"
     CDS             2054..2314
                     /locus_tag="VGO32_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200154.1"
                     /translation="MQLTPPPPPPPPPGGAGVAVGAAVPADDAEGSAPSLAEGATLAS
                     AEAEDSAEAEAVADEEVDADGWQAVSASSSTIRPDRDLTAQR"
     gene            complement(2298..2582)
                     /locus_tag="VGO32_05055"
     CDS             complement(2298..2582)
                     /locus_tag="VGO32_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV7200155.1"
                     /translation="MARATWNGVVLAESDMVEVVEGNVYFPPDAVRPEYLHPSDTHTV
                     CWWKGTASYYTVVVDGSENPDAAWYYPETSEKARTIEGFVAFWKGVTVEP"
     gene            complement(2777..4216)
                     /locus_tag="VGO32_05060"
     CDS             complement(2777..4216)
                     /locus_tag="VGO32_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEV7200156.1"
                     /translation="MINPASATAERRGIGLLALVSIGILIASMLVALRPSAALAGDPV
                     CLNPVDVEFILDNSGSMGSASGGGTRLSEAIEAAQGFVNALQANGGVGAGGLHRVGLT
                     SFNGTSTGGDLEFSIDDNLSAAAANDEIDNLTASGNTPLRDGMLTGQADLTPFTRTFV
                     DGVPVTHAYVLLSDGRPWVDQLGVTRPTAGEIASYVGSAEQAYSILLGVSDNANPPTP
                     NTLDPVLMGSLASNASNFFQITDASQLTDIFGDIATDLLCGDIDLVKTVAPGSLPVNG
                     GMVDYSFRLSHVGAQGSAPFTDVTLEDELNSVGSGIPGCSPVRGADDPGDGDALLEAG
                     ETWTFACNDVSISTDTTNWACATAEYVNSNGATTTDCDDATVVVADPTPTPTPKPPTP
                     TPTPTPEPPTPTPTPEQPTPTPTPVVTPTPEGSVGGGTGTPAASVPDTAASLPGFGGP
                     LATLVFGLILVASLGTLAYANVRAARERR"
     gene            complement(4511..5884)
                     /locus_tag="VGO32_05065"
     CDS             complement(4511..5884)
                     /locus_tag="VGO32_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEV7200157.1"
                     /translation="MAIAATETRRRFTLPDNELPTHYYNIAADLPEPLPPPLHPGTKE
                     PIGPEAFAPLFPMELIRQEVSTERFIEIPEPVRDVYRIYRPSPLIRALGLERALDTPA
                     RIYYKNESVSPAGSHKPNTAVAQAFYNRDEGIRRITTETGAGQWGSALAFAGSLFGIE
                     TKVYMVKVSYEQKPFRRSFIETFGAQVVASPSTETNAGRQILAADPDSTGSLGIAISE
                     AIEEAASREDTKYSIGSALNHVLLHQTVIGQEAIKQMEMAGDAPDVVIGCAGGGSNFS
                     GIAFPFLREKLGGRSETRFLAVEPASCPSLTRGEYRYDFGDSVGMTPLLKMHTLGHEF
                     RPAPIHAGGLRYHGMAPLVSHLYALGFIESRAYAQNTCFEAAVQFARTEGLIPAPESS
                     HAIRAAIDEALEAREAGEERVILFNLSGHGYFDMAAYDQYLAGELVDVEFEPMAAADA
                     EAEPAVA"
BASE COUNT          978 a         2121 c         2227 g         1048 t
ORIGIN      
        1 cgcagctggg gaaacacgtt cgcggccagc cattccaggc ggctcgtgtc gttcaccccg
       61 cgcacgctga agatgacgtg ctgggcgccg gcctcggcca gggcccccca gcgctcgacg
      121 atggcctcgg ccgagtctcg ctcggtgtcc accgacttga ggaccgagcg ctcgatctcc
      181 tcgtatggcc ggccgagtcg ctcgcagtgc gcccgcagga cggcgtactt gtgccggagc
      241 gtgtcggggt cgccgaagac gttggacgcg tcggcgtact gggccgccag gcgcagcgtc
      301 ttccgctcgc cgccgccgcc gatcatgatc ggcacccgcg gccggctcaa tgattgcggc
      361 gagttcagga gccgcgtcgc ggtgtagtgc ttgccgtcgt gccgcgcctg gctcccgctt
      421 ccccccgacc acatggcatg cgccatctgg agcgtctcct cgagcatctc gaaccgctcg
      481 ccgagcggcg ggaacggcca ccccagaccc atggactctt cctcgttcca ggcggcaccg
      541 atcccgaacc acgctcggcc gccggacagc acgtccaggg tggtggtcgc cttgatccac
      601 aggcccggcg cgcgatagtg gatcccgccg accatcagac cgatgcgggc acgctccgaa
      661 tgggcggcca tgaagcccag cgcggtctgg ccgtccagca tgggagcctc cggcggaccc
      721 actccccgga tctggaagaa gtggtccatg acccagatcg agtcgaatcc gacctcgtcc
      781 gccgtgcgca cgacccggcc aagggtgggg ccgagcgcct ccgcgccgcc gggccacgtg
      841 aacgaactga tctgaagtcc gaacttcaca aggggtcgag cagcctccat gatgggaact
      901 gcacggacga ggggagcgtc actggcacga gatgacgatc tcacgaatgg ggtctgctgc
      961 gctgctggcc gccgtcatct gcggctgcag catcctgccg ggaatgggtg gctcacccga
     1021 ggtcgcgggc cgcgagttcg tctccacctc agtcaccgtg gacgggatat cccaacccct
     1081 ggtcgatgga acgcagctcc ggatcggctt cacggccgac gggatgctca tggcatcggc
     1141 cggatgcaac accttcgccg ccatgtatcg gatcgatggc ggggtgctgg ccatcaacga
     1201 aggagccgtg accgagatcg gctgcccatc ggaccttgcc gatcaggacg cctgggtgtt
     1261 cgcattcctc ggcgcccagc cgttgatctc ggtcgacggc gatgagcttg tgctcgagga
     1321 cgacaacacc gtcatcacgc tgctcgaccg ggaagtcgcc gatcccgatc ttccgatcgt
     1381 cggtccggtc tgggtggtca ccgggctcat cgtgggcgac gccatttcca gcatccccga
     1441 cggcgtcgtg gcgaccctcg tcttcaccga ggacggcctg gtcatggtca ataccggctg
     1501 caactcggga agcggcgcgg ccgagattcg gaccggcacg gagacgatcc tgttcggtga
     1561 ggtcgcgctg acccggatgg cctgccagcc cccggcgtcg agcatggaag cggccgtcct
     1621 ccgagtcctg aatgccgacg tggtcaggta cgcgatcgag gccagcaccc tggacctcag
     1681 catcgcgggc tacgggcttc agctcagtgg ggccgccgtg gactagcggg aggcgccctc
     1741 caccgtcacg gtcaaccgtt cgcaaccacc gcaggcgctc aggttgaggg ccagggtggt
     1801 tccgctgccg acggcgaccg ccggggataa ggtcacgatc cgttcgcccg atccggcgcc
     1861 aggggacccg gtggtgtagt agttcagcat attgatcgtg aggatggtcg taccgccggc
     1921 cgtgacggcc gcggtccccg ttccctcgtc agggaagaac agcacgagat cgacgatgca
     1981 caggccactc gacgtcgccg gactcgcggt ccgggtgacc atgagaaacg tgttcggcga
     2041 cagcgagcct gaggtgcagt tgacccctcc accgcctcct cctccgccgc ccggaggcgc
     2101 cggcgttgcg gtgggcgccg ccgtgcccgc ggacgacgcc gaaggctcgg ctccgtcgct
     2161 ggcggaaggt gcgacgctgg cgtcggcgga ggccgaggac tcagcggagg ccgaggccgt
     2221 cgcggacgag gaggtcgatg ccgatggctg gcaggcggtg agcgcgagca gcagcacgat
     2281 caggccggac cgagatctca cggctcaacg gtgacgccct tccagaacgc gacgaacccc
     2341 tcgatagtcc tcgccttctc ggacgtttcg gggtagtacc acgccgcgtc ggggttctcc
     2401 gaaccgtcaa cgacgacggt gtagtagctc gccgtgccct tccaccagca gacggtgtgg
     2461 gtgtcgctgg ggtggaggta ctcggggcgc accgcgtccg gaggaaagta cacgttgccc
     2521 tcgacgacct cgaccatgtc gctctcggcg aggacgacgc cgttccacgt tgcgcgagcc
     2581 atgcgcctag cgtactgcgg atcggcagaa caccagtcga cacaacagag aacggggtac
     2641 ggaatactcc gtaccccgtc cctgccaggg gggctggcga tattgggcgt atcagccgga
     2701 ccgtccggcc gaggcgccga gctcttggtt tggtcggtca ggcccccggc ggacccgagg
     2761 gcctgaccgg atttgcctag cggcgctcgc gagctgcgcg cacgttagcg tacgccagcg
     2821 ttccaagcga ggccaccagg atgaggccga agacgagcgt cgccagtggt ccaccgaatc
     2881 ccggcagact ggctgccgtg tcgggcaccg atgccgcagg cgttccggtc ccgccaccca
     2941 cgctgccttc gggcgtcggc gtgacgaccg gggtcggagt cggggtcggc tgctcaggcg
     3001 tcggagtcgg ggtcggcggc tcaggcgtcg gggtcggcgt cggagtcggc ggcttaggcg
     3061 tcggagtcgg cgtcggatcc gccacgacaa cagtcgcgtc gtcgcagtca gtggtggtgg
     3121 cgccgttcga gttcacatac tcggccgttg cacaggccca gttggtcgtg tcggtcgaga
     3181 tgctgacgtc gttgcaggcg aaggtccagg tctccccggc ctccagcaag gcgtctccat
     3241 cccccggatc gtccgcaccg cgaacgggcg agcagccggg gatcccgctg ccgacggagt
     3301 tcaactcgtc ctcgagcgtg acgtcagtga agggcgcgct gccctgggca ccgacgtgcg
     3361 acaggcggaa gctgtagtca accatcccac cgttcaccgg aaggctgccc ggcgcaacag
     3421 tcttgacgag gtcgatgtca ccgcacagca ggtcggtcgc gatgtcgccg aagatgtctg
     3481 tcagctggct ggcatcggtg atctggaaga agttgctcgc gttcgaggcg aggcttccca
     3541 tcaggaccgg gtccagcgtg ttcggggtcg gcgggttcgc gttgtcggac acgccgagga
     3601 ggatcgagta cgcctgctcg gcggagccga cgtacgaggc aatctcacca gcggtcgggc
     3661 gggtcacgcc gagctggtcc acccacgggc ggccgtccga cagcagcacg taggcatggg
     3721 tgacaggaac accgtcgacg aacgtgcgcg tgaacggtgt caggtccgcc tggccggtca
     3781 gcatgccgtc ccgaagcggg gtgttgccgg acgccgtcag gttgtcgatc tcgtcgttcg
     3841 ccgcggcggc gctcaggttg tcgtcgatcg agaactcgag gtctccgccg gtcgaggtcc
     3901 cattgaagga ggtcaggccg acgcggtgca gaccgcccgc gcccacgcca ccgttggcct
     3961 ggagggcgtt cacgaagccc tgggccgctt caatggcctc gctgaggcgg gtcccgccac
     4021 cggaggccga gcccatgctg ccggagttgt cgaggatgaa ctcgacgtcc accgggttca
     4081 gacagacggg gtcgcccgcc aaggcggctg atgggcgaag cgccaccagc atcgatgcga
     4141 tgaggattcc gatggatacg agggcgagga gtccgattcc tcgtcgctcg gccgtggcac
     4201 ttgccgggtt gatcaccgga tctgtccctc cctgggagat aaaggtgccg cccggacgat
     4261 ggcggcaatg atgcagaggc ccctttcggg gcttccgcca cgctatcagc ggggtccggg
     4321 gagggtctag tacctccggc ccatctcggt ggtggggtcc gttttgctcc tgacgggtct
     4381 ggtgaggtcg gccccgaaaa tgcggtgcgc ggaggcaccg aggagtacta gttctcgggt
     4441 accaggaggc ctcttgggag gcgccggggc ccgagggcct gggccccggc gctgaggatc
     4501 gaccggcggc ctacgcgacg gccggctcgg cctcggcatc cgcggccgcc atcggctcga
     4561 actcgacatc aacgagctca ccagccaggt actggtcgta ggccgccatg tcgaagtagc
     4621 cgtgtcccga caggttgaac aggatcaccc gctcctcacc tgcctcgcgc gcttcgagcg
     4681 cctcgtcgat ggctgcccgg atggcgtggc tcgactcggg cgccgggatg agcccctcgg
     4741 tccgggcgaa ctggacggcg gcctcgaagc acgtgttctg ggcatacgcc ctggactcga
     4801 tgaagcccag cgcgtacagg tgcgagacca gcggcgccat gccgtggtag cgcagcccgc
     4861 cggcatggat cggggctggc cggaactcgt ggccaagggt gtgcatcttc agcagcgggg
     4921 tcatccccac cgagtcgccg aagtcgtacc gatactcccc gcgggtcagg ctcgggcagg
     4981 acgccggctc gaccgcgagg aaccgggttt ccgagcggcc gccaagcttc tcgcgcagga
     5041 acgggaaggc gatgcccgag aagttcgagc ccccgcccgc acagccgatg acgacgtcgg
     5101 gtgcgtcacc cgccatctcc atctgcttga tcgcctcctg accgatgacg gtctggtgga
     5161 ggaggacgtg gttcagcgcg cttccgatcg agtacttcgt gtcctcgcgg ctggccgctt
     5221 cctcgatggc ctcgctgatc gcgatcccca ggctgccggt cgagtccggg tcggcggcca
     5281 ggatctggcg accggcattc gtctcggtcg acggcgaggc gacgacctgc gcgccgaagg
     5341 tctcgatgaa gctccgccgg aacggcttct gctcgtacga gaccttgacc atgtagacct
     5401 tggtctcgat cccgaacagc gacccggcga acgctagggc gctcccccac tggccggcgc
     5461 cggtctcggt ggtgatgcgg cggatgccct catcccggtt gtagaacgcc tgcgccaccg
     5521 ccgtgttggg cttgtgcgag cctgcagggg agacgctctc gttcttgtag tagatgcggg
     5581 ccggggtgtc gagcgcgcgc tccaggccca gggcccggat caacggggag gggcggtaga
     5641 tgcggtacac gtcccggacc ggctccggga tctcgatgaa ccgctcggtg ctgacctcct
     5701 ggcggatgag ctccatcggg aacaggggcg caaaggcctc cggaccgatg ggctctttcg
     5761 tgcccgggtg gagagggggc gggagcggct ccggcagatc ggccgcgatg ttgtagtagt
     5821 gggtcggcag ctcgttgtca gggagcgtga accgacgccg ggtctccgtg gctgcgatgg
     5881 ccatgtgatg gaacctccga gaacaacacg acccctccgt cccaaaggac gagggggtct
     5941 cccgtggtgc cacctttgtt ccccggtgtt ctcgaccggg ctcattcgac cacggctgcg
     6001 gttgcccgcc gcgatggcct cggctttggt atcggtgcct ctccgccggg gttactgccg
     6061 cggaaccgat gtcatcggtc tgcgggttcg ctcccggagc tcacgggtcc attcgtcggt
     6121 cgcgccgccg ccggggtctc actgctcccg gctccctgga ggctcgcctg accgggtact
     6181 cgtcccgatc aacgccctat tcaactgcgg cgcgaacgct acgcccagtg tgcagggggt
     6241 gtcaatcggt gaggcgcacc tgatgcgagc tcgaggcaac tgatgcggtt ttgtcatgcc
     6301 cacgaccgtg cccgcaaccg cctgcccggt ccccctcggg cacgatgacc agcctgttgc
     6361 agccctcggg ctga
//
LOCUS       DASYGU010000181         5904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_801365, whole genome shotgun sequence.
ACCESSION   DASYGU010000181 DASYGU010000000
VERSION     DASYGU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801365"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..76)
                     /gene="rpmJ"
                     /locus_tag="VGO32_05070"
     CDS             complement(<1..76)
                     /gene="rpmJ"
                     /locus_tag="VGO32_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV7200158.1"
                     /translation="MKVSASVKPRCERCKVIRRSGVVLV"
     gene            complement(73..291)
                     /gene="infA"
                     /locus_tag="VGO32_05075"
     CDS             complement(73..291)
                     /gene="infA"
                     /locus_tag="VGO32_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV7200159.1"
                     /translation="MPKKDAIEVEGEVILPLPNAMFKVRLENGHEVLAHISGKLRKNF
                     IRILPGDTVRVELSPYDLTRGRITYRMR"
     gene            complement(415..1215)
                     /gene="map"
                     /locus_tag="VGO32_05080"
     CDS             complement(415..1215)
                     /gene="map"
                     /locus_tag="VGO32_05080"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV7200160.1"
                     /translation="MGIPLRHPSATIKRPEEVARMRHAGQILVGLLDRLEGALGTGVT
                     TAELDAIAVDHIRDAGAVSSFKGYGSNPPFPGTICTSLNEEVVHGIPSSRRRIQDGDL
                     IGIDVGCIFEGWHADCARTFAIGSVEPAERDLIDHTREALSAGIRAAMPGNRLGDVGA
                     AIEAVANAHGYGIVRPFVGHGIGSSMHEEPNVPNYGRPGTGMRIELGMCLAIEPMFNL
                     GGDEVRLLDDGWTVVTADGTRSAHFEDTIVIGPEGAERLTARPVEARA"
     gene            complement(1224..1877)
                     /locus_tag="VGO32_05085"
     CDS             complement(1224..1877)
                     /locus_tag="VGO32_05085"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV7200161.1"
                     /translation="MMVVLLGPVAAGKGTQASRAAEEHGMLHLSTGDLFRRAIRAGTP
                     LGEIAQGYMRRGELVPDEITVSMVGEALGEPSAAGGVVFDGFPRTLEQAKALDLLLAE
                     GGSAISTAILIEVPEEVLVDRVAGRWVCPECGTPYHATDDPPREPGVCDRDGAALTQR
                     DDDRPEVFRARLREQIPPMEDVADYYEGQGKLARVDGRQPIGAVSAAIRDVLADPRD"
     gene            complement(1874..3157)
                     /gene="secY"
                     /locus_tag="VGO32_05090"
     CDS             complement(1874..3157)
                     /gene="secY"
                     /locus_tag="VGO32_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV7200162.1"
                     /translation="MIQSVVTALQAPDIRRKILFTAFILLIFRVLTAVPVPNVDQARL
                     AELFATNQLLALLDIFSGGGLATASIIGLGLNPYINASIIMQLMQGVIPRLGELQREG
                     EYGRNKLQQYTRYLTVPLAFAQGFGFSILLQNAGVIPVSPFFSFETFSLLIAWTAGTI
                     LLMWLGELISERGLGNGISFIIFAGIVGRLPDQAGPILSAPDGLLRIAPFIVVAVVII
                     AAVVFVNEGQRRVPVQYASRVRGRRMYQGQTQYLPMKVTMAGVIPIIFAVSILLFPAQ
                     IAQYFTTSSSEIVRDVATWISTVLSPQTPLVYGTLYFSLVVFFTYFYTSFQFKPDQTA
                     DFLRKNGGFIPGIRPGKPTEDFLARVANRITLGGALFLGAIAVTPLIVSAFIPGTEAI
                     RLGGTSVLIVVAVVVETMKQLEAQMMMRNYEGFIR"
     gene            complement(3154..3858)
                     /gene="rplO"
                     /locus_tag="VGO32_05095"
     CDS             complement(3154..3858)
                     /gene="rplO"
                     /locus_tag="VGO32_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV7200163.1"
                     /translation="MKLHDLKPAPGSHTPARRVGRGHGSGKGKTAGRGTKGQKSRAGS
                     SIPAWFEGGQTPLHVRTPKLHGFRNRGRVAYTAVNLAAMAEAEAGTLITPDVLAHDGL
                     IRRSKTKGLPVKVLGKGDAPKGVTVHAHAFSRSALDKLAAAGSTAQRISWPDGDPIDE
                     EAEATAAAEAKATNEAKAARRLRKRGKAGTPKAEAADAADAEEPAEPAGKTDEAPPAT
                     DAANDAEEDDGEGPTA"
     gene            complement(3855..4082)
                     /gene="rpmD"
                     /locus_tag="VGO32_05100"
     CDS             complement(3855..4082)
                     /gene="rpmD"
                     /locus_tag="VGO32_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV7200164.1"
                     /translation="MAARRTSSNPRTIKVTWIRSEIGHKAAARGTIRALGLRRLHHTV
                     EVADTPQIRGMLRRVAFLIEVVEPGETEPSS"
     gene            complement(4162..4659)
                     /gene="rpsE"
                     /locus_tag="VGO32_05105"
                     /pseudo
     CDS             complement(4162..4659)
                     /gene="rpsE"
                     /locus_tag="VGO32_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(4672..5031)
                     /gene="rplR"
                     /locus_tag="VGO32_05110"
     CDS             complement(4672..5031)
                     /gene="rplR"
                     /locus_tag="VGO32_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV7200165.1"
                     /translation="MIKKTSRDAQRRKRHARLRLRLSGSVDRPRLSVFRSTRFIYAQV
                     IDDSTGRTLASASSREPSLAGTSGKVAAAESVGKALAERARAAGVTQVVLDRGGYRYH
                     GRVRSLAEGARQGGLDL"
     gene            complement(5034..5585)
                     /gene="rplF"
                     /locus_tag="VGO32_05115"
     CDS             complement(5034..5585)
                     /gene="rplF"
                     /locus_tag="VGO32_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV7200166.1"
                     /translation="MSRIGRLPIPIPDGVEVSLEGQHVRIKGPLGTLERDLHPEMTIL
                     QEDGALRIERPSDAPRHRALHGLTRSLVANMVTGVTSGFTKVLEISGVGYRAQLQGEM
                     LVLALGFSHPVEVKPPDGISFSVETPTRLAVSGADRELVGQVAASIRARRKPEPYKGK
                     GIRYAGEQIRRKAGKAGKVGGAA"
     gene            complement(5592..>5904)
                     /gene="rpsH"
                     /locus_tag="VGO32_05120"
     CDS             complement(5592..>5904)
                     /gene="rpsH"
                     /locus_tag="VGO32_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV7200167.1"
                     /translation="SRVKTEIARILVEEGFIASFENRVEEGHDHLVLTLKYVEGKTPV
                     VTGLKRISKPGLRVYARKTEIPRVLGGLGVAILSTSHGIMTGQQARKMNLGGEVLCYV
                     W"
BASE COUNT          882 a         2002 c         2069 g          951 t
ORIGIN      
        1 tgaccagcac cacacccgag cgccgaatca ccttgcagcg ctcgcagcgg ggcttgaccg
       61 aggcagaaac cttcatcgca tcctgtacgt gattcgaccc cgggtgaggt cgtaggggct
      121 gagctccacg cggacggtgt caccgggcag gatgcggatg aagttcttgc ggagcttccc
      181 cgagatatgg gccaggactt cgtggccgtt ctcgagtcgc accttgaaca tcgcgttcgg
      241 caagggcagg atcacctcgc cctcgacctc aatggcatcc ttctttggca accgttacgc
      301 accctcgttc gcggtctcag tactctcaat ggggggacac agggaaaggt cgccgcacgc
      361 gacgaccgga tcgaaaagta tacatcaggt gctcaaaatc gggcaatggg gtggtcacgc
      421 ccgtgcctcc acggggcgcg ccgtgagtcg ttcggcgcct tcgggaccga tgacgatcgt
      481 gtcctcgaaa tgcgcggatc gcgtcccgtc ggcggtcacg accgtccacc cgtcgtcgag
      541 gagcctgacc tcgtctccgc cgaggttgaa catcggctcg atcgcgaggc acatccccag
      601 ctcgatgcgc attccggtgc ccggccgccc gtagttcggc acgttcggct cctcgtgcat
      661 gctcgagccg atcccgtggc ccacgaacgg ccggacgatg ccgtagccgt gcgcgttggc
      721 gaccgcctcg atcgccgcgc cgacgtcccc cagccggttg cccggcatcg cggcccggat
      781 ccccgccgag agggcctcgc gggtgtggtc gatgaggtct cgctcggccg gctccacgga
      841 gccgatcgca aaggtgcggg cgcagtcggc gtgccagccc tcgaagatgc agcccacgtc
      901 gatcccgatc aggtcgccgt cctggatccg tcgacgggag gacggaatgc cgtggacgac
      961 ctcttcgttc agcgatgtgc agatcgtgcc ggggaatggc gggttggagc catagccttt
     1021 gaaggaggag accgcgcccg cgtcccggat gtggtcgacc gcgatcgcat cgagctctgc
     1081 cgtggtcacc ccggtgccaa gcgccccctc gaggcggtca aggagcccga ccaggatctg
     1141 cccggcgtgt cgcattcggg cgacctcctc gggtcgcttg atggtcgcgc tcgggtggcg
     1201 gaggggaatc cccatcgtcg gtctcagtcc cgggggtccg cgaggacgtc gcggatggct
     1261 gcgctgacgg ccccgatggg ctgccgcccg tcgacgcggg cgagcttccc ctgcccttcg
     1321 tagtagtccg cgacgtcctc catgggcggg atctgctccc gcagccgcgc gcggaagacc
     1381 tccggccgat cgtcatcgcg ctgcgtgagg gcggcgccgt cgcggtcaca gactcccggc
     1441 tcacggggcg gatcgtcggt cgcgtggtag ggggttccgc actccgggca gacccagcgg
     1501 cccgcaacac ggtcgacgag cacctcctcg ggtacctcga tgaggatggc ggtcgagatc
     1561 gcgctgcccc cttcggcgag cagcaggtcg agcgccttgg cctgctcgag cgtccgcggg
     1621 aagccgtcga acaccacgcc gccggcggca gacggctcgc cgagggcctc cccgaccatc
     1681 gagaccgtga tctcgtcggg cacgagctcg ccgcgacgca tgtacccctg ggcgatctcg
     1741 cccagcggcg tgccggcgcg gatcgcccgg cggaagaggt cgccggtgga caagtggagc
     1801 atgccgtgct cctcggccgc acgggatgcc tgggtcccct ttccggccgc caccgggccg
     1861 agcagcacga ccatcatcgg atgaagccct cgtagttgcg catcatcatc tgcgcctcga
     1921 gctgcttcat ggtctcgacc acgaccgcga cgacgatcag cacgctcgtt ccgcccagcc
     1981 ggatggcttc agtgcccggg atgaaggccg acacgatcag cggcgtgacc gcaatggctc
     2041 ccaggaacag ggcgccaccc agcgtgatcc ggttcgcgac ccgggccagg aagtcctcgg
     2101 tcggcttgcc gggccggatg ccggggatga acccgccatt cttgcggagg aagtccgcgg
     2161 tctggtccgg tttgaactgg aacgaggtgt agaagtacgt gaagaacacg accagcgaga
     2221 agtacagggt tccgtacacc agtggagtct gggggctgag cacggtactg atccaggtcg
     2281 cgacgtcgcg cacgatctcg ctcgagctgg tggtgaagta ctgcgcgatc tgggccggga
     2341 acagcaggat gctgaccgcg aagatgatcg ggatcacacc ggccatggtc accttcatgg
     2401 gcagatactg cgtctgaccc tggtacatcc gccgtccgcg cacacgggag gcgtactgga
     2461 cgggtacgcg ccgttgaccc tcgttcacga acacgacggc ggcgatgatc acgaccgcga
     2521 ccacgatgaa cggcgcgatc cggagcaggc cgtcgggcgc gctgaggatc gggccggcct
     2581 ggtccggcag gcggccgacg atgccggcaa agatgatgaa gctgatgccg ttgcccaggc
     2641 cgcgctcgct gatgagctcg ccgagccaca tcagcaggat cgtgcccgcc gtccaggcga
     2701 tgagcagcga gaacgtctcg aagctgaaga acggtgacac ggggataacg ccggcgttct
     2761 ggagcaggat cgaaaagccg aagccctggg cgaaggcgag cgggacggtc aggtagcgcg
     2821 tgtactgctg gagcttgttg cgaccgtact caccttcgcg ctgcagctcc ccgaggcgtg
     2881 ggatcacgcc ctgcatgagc tgcatgatga tgctggcgtt gatgtacggg ttcaggccca
     2941 ggccgatgat cgacgccgtc gccaggccac cgccggagaa gatgtccagc agggcgagca
     3001 gctggttggt cgcgaacagc tcggccagac gtgcctggtc gacgttcggg accggaaccg
     3061 cggtcaggac cctgaagatc aggagaatga acgcggtgaa gaggatcttg cgccggatgt
     3121 ccggcgcctg aagcgcggtg acgaccgact gaatcacgcg gtcgggccct cgccgtcgtc
     3181 ctcctccgcg tcgttggccg cgtcggtggc gggcggcgcc tcatcggtct tgccggccgg
     3241 ctcggctggc tcctccgcgt ccgccgcgtc ggccgcctcg gccttcggcg ttcccgcctt
     3301 tccccgcttc cgcaggcgac gggcagcctt ggcctcgttc gttgccttgg cctccgcggc
     3361 ggccgtggcc tcggcctcct cgtcgatcgg gtcaccgtcc ggccagctga ttcgctgcgc
     3421 cgtcgaaccg gcggcggcca gcttgtctag cgccgagcgc gagaacgcgt gggcgtggac
     3481 ggtgacccct ttcggggcgt cgcccttgcc caggaccttg accgggaggc ccttggtctt
     3541 ggaacgacga atcagcccat cgtgggcgag cacgtccggg gtgatgagcg tgccggcctc
     3601 ggcctcggcc atcgcggcga ggttcacggc ggtgtaggcc acccggccac ggttgcggaa
     3661 gccgtggagc ttgggcgtgc ggacatggag tggcgtctgg ccaccctcga accacgcggg
     3721 gatcgaggag ccggcgcgcg acttctggcc cttggtgcca cgtccggcgg tcttgccctt
     3781 gcccgagccg tggccccgcc cgacgcgacg ggcgggggta tgcgaccccg gagccggttt
     3841 caggtcatgg agcttcatga cgaaggctcc gtctcgccgg gctcgacgac ctcgatgagg
     3901 aacgcgaccc ggcgcagcat gccgcggatc tggggcgtgt ccgccacctc gacggtatgg
     3961 tgcagccgcc gcagcccgag cgcccggatc gtgccccgcg cggccgcctt gtggccgatc
     4021 tcggagcgga tccaggtcac cttgatcgtg cgcgggttgc tggacgtgcg cctagcggcc
     4081 acggttccct cccgggaagt tcatcggctc cggccgctcc acgtagacgt gaggctcgcg
     4141 ggccgccgcg gcccctcgct tgcccagcag ctgctcggcg gtcttgcccc gccgcccggc
     4201 gatctcgtca gccgactgga ggtgggacag gcagtcgagc gtggcccgca cgacgttgac
     4261 cgggttggtg ctgcccatcg tcttggacag caggtcgcga atgccggcca cctcgaccac
     4321 ggcacgcacc gagccgccgg cgatgacgcc ggtgcccgcg gtcgccggtc ggaggaggac
     4381 gcgggacgca ccgaagtcgc gctgaacggt gtgcgggatc gtgctgccca cgagcggcac
     4441 ccggatcagg tgcttcttcg cgtcctcgac gcccttgcgg atcgcctcgg ggacttcgcc
     4501 cgccttgccc agcccagcgc cgaccacgcc ccggccgtca ccgaccacga cgatggcgct
     4561 gaagctgaag cggcggccgc ccttgacgac cttcgcgacg cggttgatct ggacgacctt
     4621 ctcttcgagg gtcagcttgg tgggatcgat acgaggcatg gtttctccgc tctacaggtc
     4681 gaggccgccc tggcgggcgc cctcggcgag cgagcgaacc cgcccgtgat atcgataacc
     4741 cccgcggtcg aggacgacct gggtcacccc cgcggcgcgg gcgcgctcgg ccagggcctt
     4801 gcccaccgat tcggcggcgg ccaccttgcc ggaggtgccg gcaagtgacg gctcccggct
     4861 cgaggccgag gccagcgtgc ggccggtgct gtcgtcgatc acctgggcgt agatgaaccg
     4921 cgtgctgcgg aacacgctca ggcgtggtcg atcgacactg cccgacaggc gaagccggag
     4981 gcgggcgtgc cgcttgcgcc gctgggcgtc gcgcgaggtc ttcttgatca tggctacgcg
     5041 gctcccccga ccttgccggc cttgcccgcc ttgcgccgga tctgctcccc ggcgtagcgg
     5101 atgcccttgc ccttgtacgg ctcgggcttg cgtcgcgccc ggatggaggc ggcgacctgg
     5161 ccgacgagct cgcgatcggc gccgctcacg gccagtcggg tgggggtttc gacgctgaaa
     5221 gagatgccgt ccggcggctt gacctcgacc ggatgggaaa agcccagggc cagaacgagc
     5281 atttcgccct gcagctgggc acggtagcca acgccgctga tctcgagcac cttggtgaag
     5341 ccgctcgtca ccccggtgac catgttcgcg accagcgatc gggtcagccc gtggagggcc
     5401 cggtgccgcg gggcgtccga gggccgctcg atgcgtagcg cgccgtcctc ctggaggatg
     5461 gtcatctcgg gatgcaggtc acgctccagg gtcccgagcg ggcccttgat acggacatgc
     5521 tggccctcga gggacacctc gacgccgtcg gggatgggaa tgggcaggcg cccgatgcga
     5581 gacatcgatc cctaccagac gtagcagagc acttcgccgc cgagattcat cttccgagcc
     5641 tgctggccgg tcatgatccc gtgggaggtg gagaggatgg ccacgcccag gccgccgagg
     5701 acgcggggga tctcggtctt gcgggcatag acccgcagcc cgggcttgct gatccgcttc
     5761 agaccggtga cgaccggggt cttgccctcg acatacttca gggtgaggac caggtggtcg
     5821 tgaccctctt cgacccggtt ctcgaaggag gcgatgaagc cctcctcgac caggatgcgg
     5881 gcgatctcgg tcttcacccg tgat
//
LOCUS       DASYGU010000182         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_903710, whole genome shotgun sequence.
ACCESSION   DASYGU010000182 DASYGU010000000
VERSION     DASYGU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903710"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1812)
                     /locus_tag="VGO32_05125"
     CDS             complement(154..1812)
                     /locus_tag="VGO32_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7200168.1"
                     /translation="MGSLASYRRVFANPALARLMIGEFVSSIGDWLYLVALLVLVYRA
                     GVSPVVLGIVGAARIVPYILLSVPAGIAADRYDRRLILIVTDVARGVIMVVLTALAVV
                     DGPIIAIVALSLLAACFSAFFSPAIGSLIPTLVRDESELGPANAAWSSLDNLAFIIGP
                     AVAAILIAVGSIPLAFFLNAISFGIVAAVLWRLPRDPDRGRAREAVAPEAPRPSFRDA
                     VRPVIRPLSGLGLINLVDGFVSGGLAVLTVVIAIEVLGSGDAGTGWLNAAIGLGGLIG
                     AVLAGPMTLRRRLDIPLAVGGVTLGVGVILVGQSGVLLAAIAAMAVATAGMLVLEVVA
                     TTIFQREVPDAIRGRTIGAMDTVTVSAYALGAFLAPILASILTPVVVLLGFGVAMVAA
                     TLIGTIVVGRSAAPTLDPGAERFVRLPVFDGLSPASLEQAAGHLTRIRVGAGEVVVRQ
                     GEPADRFFHVLDGAFEVTQVKDPGGAPRFLRTLGADDVFGEIGLLRAVPRTATVTAAG
                     EGVLLALDGHRFLELVSAGPGLTSRLLDTHRGAAARLDAPAEEG"
     gene            1850..2905
                     /locus_tag="VGO32_05130"
     CDS             1850..2905
                     /locus_tag="VGO32_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEV7200169.1"
                     /translation="MPVPVIRSDAHLAHDGLIEVDFGKPIPGFEVPARAIEIEQALSA
                     TGSYRFEEPSHHGEQPILAVHDQALLDVLATAWEDALAAGVTDGTQPLIPGTLLTTAY
                     AAGHDTGLPGPGYSLFAYTFDTATPIVAGTYRAARVSVDIALTALDRSQGGAHLAYGL
                     CRPPGHHAGKALYGGYCFFNNAAIVAQALSAGGASRVAILDIDYHHGNGTQQLFWDRH
                     DVLYVSLHGDPTRAYPYYSGYAREVGFGAGEGMNVNLPLPAGTDGNAYLAALETGLQA
                     IRDHDPDAPLVLSLGFDTFERDPIADFAITTDDYRRIGAAIGELGLRVIALQEGGYAV
                     DAIGANAVSFFGGLQGQ"
     gene            2918..>3299
                     /locus_tag="VGO32_05135"
     CDS             2918..>3299
                     /locus_tag="VGO32_05135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7200170.1"
                     /translation="MEPDSFLGREIAGYRIDEIIGRGGMGVVYRAEHLRLGRTVALKV
                     VAPQIAREEKFRERFLRESRTAGTLDHPNVVPMYDAGEADGVLYIAMRFVDGVDLATL
                     IERSGPLEPARTARIVAQVASALDA"
BASE COUNT          562 a         1154 c         1162 g          421 t
ORIGIN      
        1 acctcgcccc gccgaaaccg ccgtcggcgg aggtggcgga ccatgtcctc gaggtcaccg
       61 ggctccgcgc tcacgaacag ggggcagcgc tggagcaatt gggcggtctg gtcgtcggcc
      121 atatcagtca tcgagcgcca cgcgccccca tcttcagcct tcctcggccg gcgcgtcgag
      181 gcgcgcggcc gcgccccgat gggtatcgag aaggcgggac gtgagacccg ggcctgcgct
      241 gaccagctcc aggaaccggt ggccgtcgag tgccaggagg acgccctcgc cggcggcggt
      301 cacggtggcg gtccgcggga cggcacgcag cagaccgatc tccccgaaca cgtcgtccgc
      361 gccgagcgtc cgcaggaagc gaggtgcgcc acccggatcc ttgacttggg tcacctcgaa
      421 cgcgccatcg agcacgtgga agaagcgatc tgccggttcg ccttggcgaa caaccacttc
      481 gccggccccg acccggatcc gagtcaggtg gccggccgcc tgctccaggg aggcaggcga
      541 caacccgtcg aacacgggga gtcggacgaa tcgctcagca cctggatcga gggtgggggc
      601 cgctgagcgc ccgacgacga tagttccgat cagcgtggcg gccaccatgg ccacgccgaa
      661 gcccagcagc acgaccacgg gggtcaggat cgacgccagg atgggggcca ggaaggcacc
      721 gagggcgtac gcggacacgg tgacggtgtc catggcgcca atcgtccgac cgcggatcgc
      781 gtcaggcacc tcgcgttgga agatggtggt ggccaccacc tcgaggacga gcatgccggc
      841 ggtggccacc gccatcgcgg caatggccgc cagcagcacc ccggactgac cgaccaggat
      901 gacgcccacg ccgagcgtca cgccgccgac cgcgagcgga atgtccagcc gacggcggag
      961 ggtcatcgga ccggcgagga cggcgccgat caggccgccg aggccgatcg ccgcgttgag
     1021 ccagccggtg ccggcatcgc cggagcccag gacctcgatg gcgatgacca cggtcaggac
     1081 cgccagcccg ccggacacaa agccgtcgac caggttgatc agcccgagtc cggagagagg
     1141 ccggatcacg ggccgaaccg cgtcgcggaa tgagggccgc ggcgcctcgg gcgcgaccgc
     1201 ttcccgggca cgaccccgat ccggatcgcg tggcagccgc cacaggacgg cggccacgat
     1261 cccgaagctg atggcattca ggaagaacgc gagcgggatg ctgccgaccg cgatcaggat
     1321 ggccgccacc gccggaccga tgatgaacgc caggttgtcc aggctggacc acgcggcatt
     1381 ggccggtccc agctcactct cgtcccgcac cagggtggga atgagtgagc cgatcgcggg
     1441 cgagaagaag gccgagaagc aggctgccag caggctgagc gcgacgatcg cgatgatcgg
     1501 gccgtcgacc acggccaacg cggtgagcac gaccatgatc acgccacggg ccacgtcggt
     1561 cacgatgagg atgaggcggc ggtcgtagcg gtccgcggcg atgccggccg ggacggagag
     1621 gaggatgtag ggcacgatcc gcgccgcacc cacgattccg agcacgaccg gtgacacgcc
     1681 ggcgcggtag acgaggacga ggagcgcgac caggtacagc cagtcgccaa tggacgagac
     1741 gaactcgccg atcatgagtc gggcaagggc cgggttggcg aagacgcgcc ggtagctggc
     1801 gaggctgccc atcggtcggc gcagtctacc cgtacactcg ggggcgccca tgccggtccc
     1861 cgtcattcgc tcggacgctc acctggcaca cgacggcctc atcgaggtcg acttcggaaa
     1921 gcccatcccc ggtttcgagg tgccggcccg cgccatcgag atcgagcagg cgctgtccgc
     1981 gaccgggagc taccgcttcg aggagccgag ccaccacgga gagcagccga tcctcgccgt
     2041 ccatgaccag gccctcctcg acgtgctggc caccgcctgg gaggacgcgc tcgccgcggg
     2101 tgtgaccgat ggcacccagc cgctcatccc cggcaccctg ctgacgaccg cctatgccgc
     2161 gggccacgac accggactcc caggcccggg atacagcctg ttcgcctaca cctttgacac
     2221 ggcgacgccc atcgtggccg gcacgtaccg ggccgcccgg gtgtcggtgg acatcgcgct
     2281 cacggccctc gaccggtcgc agggcggtgc ccacctcgcc tacggtttgt gccggccgcc
     2341 ggggcaccac gcggggaagg cgctgtacgg cgggtactgc ttcttcaaca acgccgcgat
     2401 cgtggcccag gccctgtccg cgggtggcgc ctcgcgggtg gcgatcctgg acatcgacta
     2461 ccaccacggg aacggcactc agcagctgtt ctgggatcgc cacgacgtgc tgtacgtgtc
     2521 gctccacggc gatccgacgc gcgcctatcc ctactactcg ggctacgcac gggaggtcgg
     2581 gttcggcgcc ggcgaaggca tgaacgtcaa cctgccactc cctgccggga ccgatgggaa
     2641 cgcgtacctc gccgcgctcg agaccgggct gcaggcgatc cgcgaccacg acccggacgc
     2701 gccgcttgtc ctctcgctcg gcttcgacac cttcgaacgc gacccgatcg ccgacttcgc
     2761 gatcacgacc gatgactacc gccgcatcgg cgccgccatt ggcgagcttg gattacgggt
     2821 catcgcgctc caggaaggcg gctacgcggt ggatgccatc ggcgcaaacg cggtgagctt
     2881 cttcggaggc ttgcagggcc agtagacgat gagcgcgatg gagcccgata gcttcctggg
     2941 gcgcgagatc gccggctacc ggatcgacga gatcatcggc cgcggcggga tgggcgtcgt
     3001 ctaccgcgcg gagcatctgc gcctgggccg cacggtcgcg ctcaaggtgg tggccccgca
     3061 gatcgcgcgc gaggagaagt tccgggagcg gttcctccgc gagtcacgga cggcgggcac
     3121 gctcgaccac ccgaacgtgg tcccgatgta cgacgcgggc gaggcggacg gcgtcctgta
     3181 catcgccatg cgattcgtcg atggcgttga cctcgccacc ctcatcgaac gaagcggtcc
     3241 gctcgagccg gcgcggacgg cacggatcgt ggcccaggtg gcgagcgccc ttgatgccg
//
LOCUS       DASYGU010000183         9533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_441746, whole genome shotgun sequence.
ACCESSION   DASYGU010000183 DASYGU010000000
VERSION     DASYGU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441746"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VGO32_05140"
     CDS             complement(<1..838)
                     /locus_tag="VGO32_05140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7200171.1"
                     /translation="MDALRAWLRGTEARLVGLTLLTKVFLFGWGLLIQSLAQGRPFDL
                     GQILVVWNRWDAPHYLDLAIWGYMAVDPGVTPTFGQTGDMDLMIVFYPLFPGAIATLL
                     WLVPDLVAPILVNTVALAVAVVLLYRVVARELGPVIGQRAAIFMLIFPTAYFLHIGYT
                     ESLFLALVLGSFLASRSNRWFVAGMLGGLSALTRVNGLLLIPSLAADAWTEWRATRRL
                     QLTWGWIGLVAVGFGGYLLLNLFVYGDPFAFLQIQSEHWFKSFAWPWVGIGGVIDRLG
                     DPN"
     gene            950..1026
                     /locus_tag="VGO32_05145"
     tRNA            950..1026
                     /locus_tag="VGO32_05145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Pro,seq:cgg)
     gene            complement(1005..1208)
                     /locus_tag="VGO32_05150"
     CDS             complement(1005..1208)
                     /locus_tag="VGO32_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200172.1"
                     /translation="MPGLWAKTSDAGRHAIAEAVFERIDVLGVTDYTFTLTAHAKARG
                     WDAAFGAGVVRVKEGQSGRGERI"
     gene            complement(1323..1532)
                     /locus_tag="VGO32_05155"
     CDS             complement(1323..1532)
                     /locus_tag="VGO32_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200173.1"
                     /translation="MPERRTRADVYSSARVGAATAFTLVLVVLLVLDVGVPDYDIAPG
                     ILLPVLGAICALLGLEASAFWRGVK"
     gene            complement(1546..1671)
                     /locus_tag="VGO32_05160"
     CDS             complement(1546..1671)
                     /locus_tag="VGO32_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200174.1"
                     /translation="MSVVELVLAFLAGVVMGGALDRFVLPLLVDAWIDRTRRHGR"
     gene            complement(2740..3363)
                     /locus_tag="VGO32_05165"
     CDS             complement(2740..3363)
                     /locus_tag="VGO32_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7200175.1"
                     /translation="MKLTTTTQISVDGVMQGPGGPAEDERDVFTRGGWAHFDDAAGRA
                     MDEIFLRADAFLFGRRTYEIFAGSWGSWPDPGDSPIWTALHTLPKYVASKTLTNPQWT
                     GTTVLAGDIAAAIRELKAKPGRDLQVQGSGALIRWMLDHDLVDEMTLFTFPVVVGQGS
                     RLFPETGRDWALDLVNARTTPGGITIQTYRPLGRRPEYAGSTADEAR"
     gene            complement(3857..4099)
                     /locus_tag="VGO32_05170"
     CDS             complement(3857..4099)
                     /locus_tag="VGO32_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7200176.1"
                     /translation="MTRRPRHAGGATDREVGVVAAVLATGSEKGAAHRLGLSHSTVKH
                     HLANARSKLGATSTAQLVWILASRLPAPDDMAQPDD"
     gene            4288..4653
                     /locus_tag="VGO32_05175"
     CDS             4288..4653
                     /locus_tag="VGO32_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7200177.1"
                     /translation="MVQYASVGLDVSFDALSDATRRGVLEQLGRADASITDLAQTFHM
                     TLTGIRKHVGVLEQAGLVTTEKVGRVRTCRLGPRRLDEEMAWIEQHRQLWDSRFDELD
                     KVVEELKRKEITDGRKNRE"
     gene            4631..5149
                     /locus_tag="VGO32_05180"
     CDS             4631..5149
                     /locus_tag="VGO32_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008524957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7200178.1"
                     /translation="MDARTESEPSAMKNPTAVERKSEREMVITRTVNGPARLVFEAWT
                     KPELFTRWWLPKSAGLKLRSCEMDVRVGGGYRLEFDVGAPQPVAFFGRYLEVTPHSRL
                     VWTNDEGGEGGAVSTVTLEEKGGNTLLAVHDVYASKEALDAALGSYDGMSETFEQLDE
                     LLVNLGASERRS"
     gene            5432..5920
                     /locus_tag="VGO32_05185"
     CDS             5432..5920
                     /locus_tag="VGO32_05185"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV7200179.1"
                     /translation="MTTRLPALGPRGEGWVVGQLALFALILVAGLRDLAASGSGSPWG
                     KVALVAGVVAIGIGVFVAAWGALTLRAALSPFPRPVADAPLIESGPYRLIRHPIYSGL
                     VLGAVGWGLVTSSILAIAAAGLLFLLFVGKSRREETWLSAEHPAYGAYQQRTKRFIPW
                     IY"
     gene            5978..7198
                     /locus_tag="VGO32_05190"
     CDS             5978..7198
                     /locus_tag="VGO32_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7200180.1"
                     /translation="MAGTQPGIPRRARLAAYGAVFLAFFDNFALLPLIAPRAQELGAD
                     ALGVGFAVAAYSLTNLFLNLVGGSLTDRVGRRLILLVSLAVSPICIAIYGLADSLPIF
                     LAARAIHGAFGGFLTASLFALLADLSPEGQRGKTIGRAGALIGAAAVVGPAAAGLAAR
                     EFGAQPVFLAVAAIIAVGLLLVRGAIPETLPEPARSGAPGTWRRLLADPRLRVAFLAI
                     FALEAGVGIITGFLKDGIIERQMAAGMDAERALRYATGAQGGLFSIFALVAIILMLSP
                     IARRVDRRGVLGLSLAGLVFLAGSTAIMAVDASIVTDSVAMVLYGIGFGLLFPAAAGM
                     VGIAAPLPERGRAYGLFNFSFDAGLSAGPLLAGVLAVTALGVEPFITATILIAVVVLL
                     IPLAARSRSPGPAD"
     gene            7748..8521
                     /locus_tag="VGO32_05195"
     CDS             7748..8521
                     /locus_tag="VGO32_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200181.1"
                     /translation="MDGLPVAANSATMVNTIGAGAPGHPDFGSYLGYGIPYNVVGAGT
                     PRSAVSFQYASESDVGPYPIPASPQIEGGSDRHILMWDTSACMLYELFAAQHGSGGWT
                     AGSGAIWNLHSNALRPDGWTSADAAGLPILPGLVRYDEVLAGAINHAIRFTAPSTRSS
                     HIYPARHHAGAGSSASLPPMGLRFRLKATFDMTAYSPRMRVILVAMQRYGIILADNGS
                     PWFFSGVSDARWNDSELNQLKQLRGSDFEVVDTTGFVNN"
     gene            complement(8563..9228)
                     /locus_tag="VGO32_05200"
     CDS             complement(8563..9228)
                     /locus_tag="VGO32_05200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV7200182.1"
                     /translation="MTRKSKSSAAQKRRTPDRRTSNRPLLIVGGVVAVGVVAAVIAFA
                     LASSTPAVAEPAESPIVITGTVLPPFEAEVTDDPAVGMTLPTVQGTDVTGAPMTIGPD
                     GRAKVVLLLAHWCPHCQAEVPRLVAWLAENPVPDGVDIVAVSTAADPTRTNYPASAWL
                     EREGWPIPTLNDDATLAAYRALGGPTFPGFVFVTADGVVQSRTTGEISVEEFGGWLEQ
                     IAL"
     gene            complement(9225..>9533)
                     /locus_tag="VGO32_05205"
     CDS             complement(9225..>9533)
                     /locus_tag="VGO32_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEV7200183.1"
                     /translation="YQRIAMYPLAVILLIAAIQQDWGVRPYVTALAAIGAMISGYHVL
                     VQRIPGLPTGSCSLGVSCSAIDLERFGFITIPVMALVGFVTVLVLVWTVAPREDEPEP
                     "
BASE COUNT         1422 a         3336 c         3234 g         1541 t
ORIGIN      
        1 gatttggatc tcccagccgg tcgatgacgc ccccgatgcc gacccacggc caggcgaacg
       61 acttgaacca atgctcgctc tggatctgga ggaaggcgaa cgggtcgcca tacacgaaca
      121 ggttgagcag caggtacccg ccgaagccca ccgcgaccag gccgatccat ccccacgtca
      181 actggagtcg cctcgtggcc cgccactcgg tccaggcatc agccgccagc gacggaatca
      241 gcagcagccc attgacgcga gtcagcgccg acagaccgcc gagcatgccg gcgacaaacc
      301 atcggttgct gcgcgatgca aggaacgacc cgagcacgag cgcgagaaac agcgactcgg
      361 tgtagccgat gtgcaggaaa taggccgtcg ggaaaatgag catgaagatg gccgcgcgct
      421 gtccgatgac cggtcccagc tcccgggcga cgacccgata gaggaggacg accgccacgg
      481 cgagcgcgac ggtgttgacc aggatcggcg ccacgagatc cgggaccagc cacaggaggg
      541 tggcgatcgc ccccgggaag aggggataga acacgatcat gaggtccatg tcgccggtct
      601 ggccgaaggt cggggtcacg cccgggtcga ccgccatgta cccccagatc gccaggtcga
      661 ggtagtgcgg ggcatcccat cggttccaga cgaccaggat ctggccaaga tcgaacggtc
      721 ggccctgggc aaggctctgg atgagcaggc cccacccgaa caggaacacc ttggtcagca
      781 gggtcaggcc caccagccgt gcctcggttc cccggagcca ggcccgcaac gcgtccatgg
      841 cgtcggacta tagagcgcac cggcccgtct cccgatcgtc cggggtggcg gcgtcccggc
      901 ggtcccggtt ggcacccctc ggggcggctg ctacgatgcc cgctccggtc ggggcgtggc
      961 gcagcctggt agcgcgcagc gttcgggacg ctgaagtcgg aggttcaaat cctctcgccc
     1021 cgaccagact gaccttcttt cacgcggacg acgccggccc cgaaggccgc gtcccatcca
     1081 cgggccttcg cgtgagcggt cagggtgaag gtgtagtcgg tcacccccag gacgtcgatc
     1141 cgctcgaaca cggcctcggc gatcgcgtgg cgcccggcgt ccgaggtctt cgcccatagg
     1201 cccggcaacg actccagcgt cggtcgagcg gagcgtgccg accatgcggt gatggacgag
     1261 agcgatcagt ggtgccgggt ggccggccgt gtacttgggt gagccgaccc agtagatcgg
     1321 gctcacttca cgcccctcca gaaggccgag gcctcgagac ccaggagcgc acaaatcgcg
     1381 ccgagaacgg gcaggaggat cccaggggcg atgtcgtagt ccgggacgcc gacatcgagg
     1441 acgaggagga cgaccaggac gagcgtgaac gcggtcgcgg cgccgacccg ggcggaggag
     1501 tagacgtcag cccgagtccg gcgctccggc atatcgtcgc tgtgctcatc gcccatgccg
     1561 gcgcgttcgg tcgatccagg cgtcaacgag gagcgggagc acgaaacgat cgagcgcccc
     1621 acccatgacc acgccagcca agaacgcgag gacgagctcg accacactca ccgggccacc
     1681 gcggggttga cggcgcggac gtacccggtg gggggaaccg ggagtcggtg tggatcggac
     1741 cagcgcgccg tcatgccctc aagggtggcg gctggcttcc cccgttccta ttcggccgat
     1801 tgggccatgc tctcgggttc gggcaggcgc ggctatggag cgccagtcgc tccccggctc
     1861 cgctccgggc cggctcgccg ggaagggagg cacaccgagc gaccggggtc cgtcgtgcct
     1921 agcttgatcc tggcgcctga ccgatacggc agttgagtgg accgactctg gcgttcgggt
     1981 ttgtgactgc tgggtgaggg actcctatgg ccgccgcacc cactggggac agtcctgctt
     2041 caccggatgg acgaacgcga gttgaccgca cgccatgcag tgtcccctcc aatgcttcgg
     2101 cagttccctc gcgcagggac gggcggggtg gcgggcggcg cctagcccgc agttggcaca
     2161 aagcagcgcc tccctccgcg cctcaagcag cgcctccctc cgcgcctcag ctgagggctt
     2221 cgatgtccgg ttcctcgggc gaacgagcca cactgccaga accgcaagca gaatcagaag
     2281 taggaagact tcaatgcccc atgccttcct cctgcctcga cttcccgcca cgtcggtgcg
     2341 ggcggccccc gtctgtctag gcctcgtgaa tggcctgcca gcggtccttt gtaccgggcc
     2401 actcggggtc cttcgtgaag tcaccgcatg tcttgaaggt ggtggtgatt tcaataggtc
     2461 cgttctcggg ccaagcgcca ggtgggcggc ggtcgtcacc atgatatctg ccccccgttc
     2521 cgcaactggc gctccgctcg gttaggaggc tcgtcaagca gtccgctttt cgtccgcttg
     2581 ggccatgccc taggctccgg cagccgcggc gccaggatcc acaccagctg cgccgtcgtc
     2641 gtcgccccca ccttcgatcg cgcgttcgcc aggtggtcgt tcacatggtt tgcctggcac
     2701 gaaggttgag agcggctggg gtcgcgcggg ggcgtgttgc tagcgggcct cgtcggccgt
     2761 ggaccccgcg tactcgggtc gacgcccaag cggccgatac gtctggatgg tgatgccccc
     2821 tggggtcgtc cgcgcgttga caagatcgag cgcccagtct cgaccggtct cagggaacag
     2881 ccgcgagccc tgaccgacga ccacagggaa ggtgaacagg gtcatctcgt cgacaaggtc
     2941 gtggtcgagc atccatcgga tgagcgcgcc gctgccctgc acctgcaggt cgcgtcccgg
     3001 cttggccttc agctcgcgga tggccgccgc gatgtctccg gccaggacgg tcgtgcccgt
     3061 ccattgcgga ttggtcagtg tcttcgaggc cacgtacttg ggcagggtat gcagcgcggt
     3121 ccagatgggg ctgtcgcccg gatcgggcca cgatccccac gagccggcga agatctcgta
     3181 ggtccgccgg ccgaacagga acgcgtcggc gcgcaggaag atctcgtcca tcgcccttcc
     3241 ggccgcgtcg tcgaagtggg cccaaccgcc gcgcgtgaag acgtcgcgtt cgtcctcggc
     3301 cggcccgccc ggcccctgca ttacgccgtc gacagagatc tgcgtggtgg tcgtcagctt
     3361 catggttgca gctcgctcct tgccgagttt gtggcggtcg ttccgtgctc ggccacctgg
     3421 gcgacgttca tcgtgccggc ggtcgccctt cgacgcacgg ctgatccacc ttgggcggcc
     3481 taggccctcg cactagccag ccgatctcca tcatcgcctt tccgactcac gcccaggggc
     3541 cgccgcccct tcgctgccgg gctgtgggct gtggcatcgc ggagtcagcc gctgatgccg
     3601 gtcgtgcccg tgacgcagta gccggaatca agacgcggcc ctcgccgccg ccttggcctc
     3661 cggaagccgc cggccaagga tccacactag ctgggctgtc gtctccgcgc cgaccttcga
     3721 ccgagcattc gccaggtggt gcttgacggt agagtgcgag aggccgagac ggtgggcggc
     3781 cgccgtctcc gacaccggtt cgccgcgccg gtcgttcgat gacgctttgc cggccccgat
     3841 tgtggtcccc cagctcctag tcgtccggct gggccatgtc gtcgggcgca ggcaggcgcg
     3901 aggcgagaat ccagacgagc tgcgcggtgc tcgttgcccc cagcttcgat cgagcgttcg
     3961 ccaggtggtg cttgacggtc gagtgcgaca gccccaggcg gtgggccgcc cccttctccg
     4021 atccggtggc gaggacggcg gcgacgacac cgacttcgcg atctgtggca cctccagcgt
     4081 ggcgaggccg tcgcgtcatg cggtccgcaa ccagttgaac ggctccggcg gctcggcgag
     4141 gcgcatcgtg atcggaaggt cgttggtggc ggggcaatcc ctctcgcccc gaccagactg
     4201 acctcccttc gcggacgccg cgggccccga cgaccgcgcc ccatcagtct cttgcattcg
     4261 cggatccgtt ggtgcatact gaaccgcatg gttcagtatg cgagcgttgg actcgatgtc
     4321 tccttcgacg cgctctcgga cgccacccga cgcggcgttc tggagcagct cggacgtgcc
     4381 gacgcttcga tcacggacct ggcccagacg ttccacatga ccctcaccgg aatcaggaag
     4441 cacgtcggtg tgctggagca ggccgggctc gtcaccacgg agaaggtcgg ccgggtgcgg
     4501 acgtgccggc tcggtccgcg ccgactcgac gaagagatgg catggatcga gcagcaccgc
     4561 caactctggg attcacgctt cgacgagctg gacaaggttg tcgaggaact gaaacggaag
     4621 gagatcaccg atggacgcaa gaacagagag tgagcccagc gcgatgaaga accccacggc
     4681 agtggaacgg aagtcggagc gtgagatggt catcacgcga accgtgaacg gcccggcgcg
     4741 cctcgtgttc gaggcgtgga ccaagcccga gctgttcacg cggtggtggc tgccgaagtc
     4801 ggctggcctg aagctgcgtt cctgcgagat ggatgttcgc gtcgggggcg ggtatcgcct
     4861 cgaattcgac gtcggtgccc cccagccggt ggcgttcttc gggaggtacc tcgaagtgac
     4921 gccgcactca cgcctcgtat ggacgaatga cgaaggtggc gagggtgggg ctgtcagcac
     4981 ggtgaccttg gaggaaaagg gcggcaacac gctgttggcc gtgcacgatg tgtatgcctc
     5041 gaaggaagcg ctcgacgctg ccctcgggtc ctacgacggg atgagcgaga cgttcgagca
     5101 actggacgag cttctcgtca acctgggtgc gagcgagaga cggtcatgaa gtcgtcgatc
     5161 aggccgaggc ggtgggctgc agccttctcc gatccggcaa cgaggaccgc ggcacccacg
     5221 ccgagctcac gatccgtggc gcgttcggcg gggcgggatg gctgccgcgt caggcgctgc
     5281 ggaccaggtc agaaggcccc gacgctcggc gagccgcgtg gtgatcggaa ggtcgttcgt
     5341 ggcggggcta tccctctcgc cccgaccagt cccacactgc atctcacctg gtgcccaccg
     5401 gtgcaccccg ccttgaggca acatccgcga aatgaccacc cggctcccgg ctctcggtcc
     5461 ccgcggggaa ggatgggtcg tcggtcaact ggcgctgttc gccttgatcc tcgtggcggg
     5521 actccgcgat ctggcggcct ccggatcggg cagtccctgg ggaaaggtcg ccttggtggc
     5581 gggagttgtc gcgatcggga tcggcgtgtt cgttgccgcg tggggtgccc tgacgctccg
     5641 tgctgccctc agcccctttc cgcgaccggt cgccgacgcg ccactcatcg agtccggccc
     5701 ctatcggctc atccggcacc cgatctacag cgggctggtg ctcggggccg tcggctgggg
     5761 cctggtcacg agctcgattc tggccatcgc cgcggccggg ctgctgtttc tgctgttcgt
     5821 cgggaagtcc cggcgcgagg agacctggct gtccgcggag caccccgcct atggcgccta
     5881 ccaacagcgg accaagcgct tcatcccgtg gatctactaa ccatttcggg cgagtcatga
     5941 gttgttcgga ggctgaacgc tgaccgctac cgtccctttg gccgggacgc agcccggcat
     6001 cccgcgtcgg gcacggctgg cggcctacgg ggccgtcttc cttgctttct tcgacaactt
     6061 cgcgctgctg cccctgatcg caccacgagc ccaggagctg ggcgccgacg cgctcggcgt
     6121 gggcttcgcg gtggccgcgt actcgctgac caacctgttc ctgaacctcg tcggggggtc
     6181 gctcaccgac cgggtcggtc gccggctcat cctgctcgtg tcgctggccg tctcgcccat
     6241 ctgcatcgcg atctacgggc tggcggacag cctgcccatc ttcctggctg cccgcgcgat
     6301 ccacggcgcg ttcggcggtt tcctgaccgc gtcgctcttc gccctcctgg ccgacctctc
     6361 gcccgagggt cagcgtggga agacgatcgg ccgcgccggg gcgctcatcg gcgcggcggc
     6421 ggtggtcggt ccggcggccg cggggcttgc cgcccgcgag ttcggagcgc agccggtgtt
     6481 cctcgccgtg gccgcgatca tcgccgtggg cctgctcctc gtccgcggcg ccattcccga
     6541 gacgcttccc gagcccgccc gctcgggagc ccccggaacg tggcggcgcc tcctggcgga
     6601 tccccggctg cgtgtcgcgt tcctggccat cttcgccctc gaggccgggg tcgggatcat
     6661 caccggcttc ctgaaggacg ggatcatcga gcgccagatg gccgccggga tggatgctga
     6721 acgcgccctg cgctatgcga ccggcgccca gggcgggctg ttcagcatct tcgcgctggt
     6781 ggcgatcatc ctcatgctca gcccgattgc ccgccgggtc gaccgtcggg gggtccttgg
     6841 cctctcgctg gccggcctcg tcttcctggc cgggtccacc gcgatcatgg ccgtcgacgc
     6901 gagcatcgtc accgattcgg tggccatggt cctgtacggc attggctttg ggctgctctt
     6961 ccccgccgcg gccggaatgg tcgggatcgc cgcaccgctg ccggagcgcg ggcgcgccta
     7021 cggcctgttc aacttctcgt tcgacgctgg gctgtccgcc ggacccctgc tcgccggcgt
     7081 gctggcggtg accgcgctcg gggtcgagcc attcatcacc gccacgatcc tgatcgcggt
     7141 cgtggtcctg ctcattccgc tcgctgcccg gtcgaggtcc ccgggcccag ccgactgacg
     7201 ccggatgggg aggccaattg gcctatcccc acccgacgat gggcctcttc cccgggcctc
     7261 gcccctccgc caaggtcggt tcgtgcgtcc ccgcttcgcc gtgctcggcg tcctcgtgct
     7321 cgtggtcctg cttggcctcg gcctgtccgg tcccgtcagc ccgtcccggt cgaacgccct
     7381 gccgcccagc ggtgacccgg cggcggtcca cggtgccggt gatgcaccgt acacgtcgac
     7441 cccggccgaa ccaggcggtg gatcagcggg tcaggtgccc gccgggtcca cccccggcgc
     7501 ggcgcagccg ggcacgacgg ggaccgacgg agcgaccccg gcgccatccg ccacgccggc
     7561 gcctaccagt cagtcacgga cccggccgtc cgccggcccg acggcgtcac caccaccacc
     7621 ggtcagaccg cccccggtcg tcccgacccc gccgccccca ccaccgccac caccgcctcc
     7681 gcctcctcca cccccgccga cattggcctg tccgctcttc ccggccggca acgtgtggaa
     7741 ccgccgcgtg gacggactgc cggtggccgc caactcggcc accatggtga acacgatcgg
     7801 cgccggcgcc ccgggccacc cggacttcgg ctcgtacctc ggctacggca tcccgtacaa
     7861 cgtcgtcggt gccggcacgc cgcgatcagc ggtcagcttc cagtacgcat ccgagtccga
     7921 cgtcgggccc tatcccatcc cggccagccc ccagatcgag ggcggcagcg accgccacat
     7981 cctgatgtgg gatacgtccg cctgcatgct gtacgagctc ttcgcggcgc agcacggctc
     8041 cggcggctgg acggccggca gcggggcgat ctggaacctt cattcgaacg cgctgaggcc
     8101 agatggctgg acgagcgccg acgccgcggg actgccgatc ctgcccggcc tcgtccgcta
     8161 cgacgaggtg ctcgccgggg cgatcaacca cgcgatccgg ttcacggccc cgtccacccg
     8221 ttcctcgcac atctatccgg cccgccatca cgccggggcg ggcagctcgg cctcgctgcc
     8281 gcccatgggc ctccgcttcc gcctcaaggc cacattcgac atgaccgcat acagcccgcg
     8341 gatgcgggtg atcctggtcg ccatgcagcg ctacggaatc atcttggctg acaacggatc
     8401 gccatggttc ttttcgggcg tctccgacgc gcgctggaac gacagcgagc tgaaccaact
     8461 caagcagctc cgcgggtccg acttcgaagt ggtcgatacg actggcttcg tgaacaactg
     8521 accatcggct gaccggcgct ctccaccccg gctgacggcc ggctagagcg cgatctgctc
     8581 cagccagccg ccgaattctt cgacgctgat ctctccggtc gtgcgcgact ggaccacgcc
     8641 gtcagccgtg acgaacacga atccggggaa cgtcgggccc ccgagcgccc ggtaggcggc
     8701 gagagtcgca tcgtcgttga gcgtggggat tggccatccc tcgcgttcga gccacgccga
     8761 ggccgggtag ttggtccgcg tcggatcggc cgctgtgctg accgcgacga tgtcaacgcc
     8821 gtcgggaacc gggttctccg cgagccacgc cacgaggcgg ggcacctccg cctggcaatg
     8881 ggggcaccag tgggcgagca ggaggacgac ttttgcccgg ccgtcgggac cgatggtcat
     8941 cggcgcaccg gtgacgtccg ttccctggac ggtgggcagg gtcatcccga ccgccgggtc
     9001 atcggtcact tcggcctcga agggtggcag gacggtcccg gtgatcacga tcggggactc
     9061 ggccggctcg gccacggcgg gcgtggacga cgctagcgcg aacgcgatga cggcggccac
     9121 gacgccaacc gcaaccacgc caccgacgat caggaggggt cggttggacg tccggcggtc
     9181 cggcgtccgg cgcttctggg ccgccgatga cttcgacttc ctggtcatgg ctcgggctcg
     9241 tcctctcgcg gggcgacggt ccacaccagc acgaggacgg tcacgaatcc gaccagggcc
     9301 atgaccggaa tggtgatgaa gccaaagcgc tcgagatcga tggccgagca cgagaccccg
     9361 agcgagcagc tcccggttgg gaggcccggg atccgctgca cgaggacgtg gtatcccgaa
     9421 atcatcgcgc cgatcgcggc cagcgcggtc acgtatggtc ggacgcccca gtcctgctgg
     9481 atcgcggcga tgagcaggat gacggccagc ggatacatcg cgatgcgctg gta
//
LOCUS       DASYGU010000184         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1161426, whole genome shotgun sequence.
ACCESSION   DASYGU010000184 DASYGU010000000
VERSION     DASYGU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161426"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGO32_05210"
     CDS             <1..235
                     /locus_tag="VGO32_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200184.1"
                     /translation="DPYAVIEEVVRAYDIRWVIVTLREGGTTDPLGLWNGCAARDSVD
                     NSAWSGGPDTTATWMSCDPVFEAEGVRIYAVVE"
     gene            253..1767
                     /locus_tag="VGO32_05215"
     CDS             253..1767
                     /locus_tag="VGO32_05215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7200185.1"
                     /translation="MSRPVAFALLVAVGLAAVVRLAAAFPGPLTPGDGGLIVVFVDDL
                     RRAGFVMPPVTTYNFADIPFVYPPLGLYGAALLGEVLGLSSLDSVRLVATLVSLATVG
                     AFGLLAFRLLPAAAAAGAVLMYALMPHAYDAIVAGGGVTRGSGLLFAMLAMWMAATPS
                     GVSARRAVGIGVLLGLAALSHPQTAIYGAAASTVLVFRPGGLATTAQRVVIMGVTAFI
                     VILPWLVLMTNTHGLETVLAPGNRLDPALALVLLMSLTFSGAGFTDLFLVVGTLGLFV
                     ELLRRRWRLPLLLGCLLLAGEADFIGAVPWSLLGGAAIGFVLEGIRPFVGQRHRGMAL
                     AVGLVALFAALVSSMGSVVDETSRLQRVSEDQAAAMEWARDETDPGTRFIVAAIVGWG
                     ADEISEWFPAVAQRQSVATVQGTEWLGADGYLDQAHRHRAVRLCTASTDRCMAEWADS
                     QGLADAWLFIPKGRVNGPLSPDDCCPAIRELVQDSIYYEVVFDGPGATIARPRD"
     gene            1772..2638
                     /locus_tag="VGO32_05220"
     CDS             1772..2638
                     /locus_tag="VGO32_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV7200186.1"
                     /translation="MRSRLAGLWSSRRVRLVARAFALGWIVFFWVWLIGAQSNFAEVD
                     AEAYWGLTLDNLYRGVQLGDQDAFLYSPVVAYLFLPFSALPYEVFYAILAAVNLAALV
                     WILRPELAALSLFLVPVSNEVARGNIHILIAAAIVLGFHYYGSWAWVILTKVTPGIGV
                     LWFGVRREWRSLAIALGVTGAIVAVSVVLTPGLWLRWLDVLTASVEITRPSVIEIPVL
                     PRLAVAAVLIAIAARRSWFALVPVACMLALPAIWVNSLAMLVAVVPLLRYSTTHRRPP
                     VPDEPGVPRAGD"
     gene            2743..3783
                     /locus_tag="VGO32_05225"
     CDS             2743..3783
                     /locus_tag="VGO32_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7200187.1"
                     /translation="MAQAADRNGVVQIDRLNAQAADSLGRAARRLHRLAEGGRALYRR
                     RLGTWLQLKARLEHAPNGTSGDETNPLRVEHETVVRELAIVQHRMRRLEVAERQLDLL
                     VRYLSASDANAIEPTLDTTDESAAATALRIIQAQEQERQRLAEEIHDGPAQVLTNAIF
                     QVEYLDRVVAGASAAGATRAATSSQARTELALLRSMLRDGLEEVRGFIATLRPPAVEV
                     GLSTALRDAAAEFQARNGITVRVEVDGIDTGLSPAARASVLRITQETLQNVRKHAAAT
                     EVRISLDGNRLVIADNGRGFDVMRLASGATRNFGLQFMRERAELMGSELQIESRQGEG
                     TRILLRLPEPSR"
     gene            3798..>4220
                     /locus_tag="VGO32_05230"
     CDS             3798..>4220
                     /locus_tag="VGO32_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7200188.1"
                     /translation="MKVKEENVAKAQADAAAEAEATKAEAAKTDESGKERRTGRDRRV
                     PTEKTRIVIVDTHTLFRRGVRNILDLEPDMTVVGEAGSGREAISVVEELTPDVVLMDL
                     TLPAPNGIETTQRIKRELPHTGVIVLAGGDDEDQLFDAI"
BASE COUNT          582 a         1456 c         1491 g          691 t
ORIGIN      
        1 cgatccttac gcggtaatcg aggaagtcgt ccgcgcctac gacatccgct gggtgatcgt
       61 caccctgcgc gagggcggca ccaccgaccc gctgggcctg tggaacggct gcgcagcgcg
      121 tgacagcgtg gacaacagcg catggagcgg cggaccggac acgaccgcga cctggatgag
      181 ctgcgaccca gtgttcgagg ccgagggcgt gcggatctac gcggtggtcg agtgacctcg
      241 agactcggac gcgtgtcgcg tcctgtggcc ttcgccctcc tcgtggctgt cggcctggcg
      301 gccgtcgtgc gactggcggc ggccttcccc gggcccctca caccggggga cggcggcctg
      361 atcgtggtct tcgtcgatga ccttcggcgg gcggggttcg tgatgccccc ggtcacgacg
      421 tacaacttcg ccgacatccc gttcgtctac cccccgctcg gcctgtatgg cgccgcgctg
      481 ctgggtgagg tgttggggct gtccagcctg gacagcgtcc ggctcgtcgc gacgctggtc
      541 tcgctggcga ccgtgggagc cttcggactg ctggccttcc gccttctccc cgcagccgcc
      601 gcggccggcg cggtgctcat gtacgcgctc atgccccatg cctatgacgc gatcgtcgcg
      661 gggggcgggg tcacgcgcgg ctccgggctc ctgttcgcga tgctggcgat gtggatggcc
      721 gcgacgccat ccggcgtgtc agcccggcga gcggtgggga tcggggtcct cctcggcctg
      781 gccgcgctgt cccatcccca gaccgcgatc tacggcgcgg cagcctccac cgtcctcgtc
      841 ttccggccgg gcggcctggc gacgacggcc cagcgggtcg tgatcatggg tgtgaccgcg
      901 ttcatcgtca tccttccctg gctcgtcctc atgaccaaca cgcatggact ggaaacggtc
      961 ctggccccgg gcaaccgcct ggatccggcg cttgccctgg tcctgctgat gagcctcacc
     1021 ttctccgggg cgggattcac cgacctcttc ctggtggtgg gcaccctggg cctcttcgtc
     1081 gagctcctgc gacgccgctg gcggttgccg ctgctgctgg gctgcctcct gttggccggc
     1141 gaggcggact tcatcggcgc ggtgccctgg tcgctgctgg gtggcgccgc gatcgggttc
     1201 gtgctggagg ggatccgacc attcgtcgga cagcggcacc ggggcatggc gctcgcggtc
     1261 ggcctggttg ccctgttcgc ggcgctggtc agcagcatgg gttcggttgt cgacgagacg
     1321 tcacgcctgc agcgggtgag tgaggaccag gccgcggcca tggagtgggc acgggacgag
     1381 acagacccgg gtacacgatt catcgtggcc gcgatcgtgg gctggggcgc cgatgagatc
     1441 agcgagtggt tcccggccgt ggcgcagcga cagagcgtgg cgacggtcca gggcaccgag
     1501 tggctgggcg cggacggcta tctggaccag gctcaccggc accgggcagt tcgcctctgc
     1561 acggcgtcaa cggaccggtg catggccgag tgggcggact cccaggggct ggccgatgca
     1621 tggctgttca tccccaaggg ccgggtcaat ggcccgctct cccccgacga ctgttgcccg
     1681 gccattcgcg agctcgtcca ggacagcatc tactacgagg tcgtgttcga cggacccggc
     1741 gccaccattg ctcgaccgcg cgactgaatc agtgcggtcc cggctcgcag ggttatggag
     1801 cagccggcgc gtccgcctcg tggcccgcgc cttcgccctg ggctggatcg tgtttttctg
     1861 ggtgtggctg atcggtgccc aatcgaactt cgccgaggtg gacgccgaag cctactgggg
     1921 cctgaccctg gacaatctct atcgcggcgt gcagctaggc gatcaggacg cgttcctgta
     1981 ctcgccggtc gtggcctacc tgttcctgcc gttctccgcg ctgccgtacg aggtcttcta
     2041 cgcgatcctg gcggcggtca acctggccgc gttggtgtgg atccttcggc cggagctggc
     2101 ggccctctcc ctgttcctgg tccccgtttc caacgaggtc gcccggggca acatccacat
     2161 cctgatcgcg gcggccatcg ttctgggctt ccattactac ggcagttggg cgtgggtcat
     2221 cctcaccaag gtgacgcccg ggatcggggt cctgtggttc ggggtgcgac gggagtggcg
     2281 atccctcgcg atcgccctcg gcgtgaccgg ggccatcgtc gcggtctccg ttgtcctcac
     2341 gccgggcctg tggctccgct ggcttgacgt cctgaccgcg agcgtcgaaa tcacgcggcc
     2401 ctcggtcatc gaaataccgg tcctgccccg cctcgccgtg gcggcggtcc tgatcgcgat
     2461 cgcggcccgg cgcagctggt tcgcgctggt gccggtcgcc tgcatgctgg ccctgcccgc
     2521 catctgggtg aactcgctcg cgatgctcgt cgcggtcgtc ccgctcctgc ggtactcgac
     2581 cacgcaccgg cggccgccgg tgccggacga accaggggtc ccgcgagcgg gggactagta
     2641 cttccgggtc cgaagtactg ttgaccccag gcacaggacg ccgggaggat ggcccaccag
     2701 tccggtacca gccgggcgct ggtacttcca cgaggatcgt gtgtggcaca ggccgctgac
     2761 cggaacggcg tcgtccagat cgaccgtctc aacgcgcagg ctgcggacag cctgggccgg
     2821 gctgcgcgcc gcctccatcg gttggccgaa ggtggtcgtg cgctctaccg gcgacggctg
     2881 ggcacctggc tgcagctgaa ggcccgcctg gagcacgcgc cgaacggcac ctccggagac
     2941 gagaccaacc ccctccgcgt tgagcacgag accgtggtcc gcgagctggc gatcgtccag
     3001 caccggatgc gacgcctcga ggtcgccgag cgccagctgg acctgctcgt ccgctacctg
     3061 tccgccagcg acgcgaacgc gatcgagccc accctagaca cgaccgatga gtcggcggcg
     3121 gccacggcgc tgcggatcat ccaggcccag gagcaggagc gccagcggct ggccgaggag
     3181 atccatgacg gcccggccca ggtcctgacc aatgccatct tccaggtcga gtacctcgat
     3241 cgggtcgtcg cgggcgcatc ggccgcgggc gcgacccggg ccgccaccag ctcgcaggcc
     3301 aggaccgagc tggccctgct tcgaagcatg ctccgtgacg ggctggagga ggtccgcggc
     3361 ttcatcgcga ctctccggcc gccggcggtg gaggtggggc tgtcgaccgc cctccgagac
     3421 gccgcagccg agttccaggc ccgcaacggc atcaccgtcc gggtcgaagt ggacggcatt
     3481 gataccgggc tgtcgcccgc ggcgcgggca tcggtcctgc gcatcaccca ggagacgctc
     3541 cagaacgtcc gcaagcacgc ggccgcgacg gaggtccgta tcagcctcga cggcaaccgc
     3601 ctcgtcatcg ccgacaacgg ccgtggcttc gacgtcatgc gcctggcgag cggggcaacc
     3661 cgcaacttcg gcctccagtt catgcgcgag cgcgccgagc tgatgggttc ggagctgcag
     3721 attgagtcgc ggcagggcga agggacgcgg atcctactcc gtctccccga gcccagccgc
     3781 tagcatccgg gggcggagtg aaggtcaagg aggagaacgt ggcaaaagcg caggcagacg
     3841 cagcggccga ggccgaggcg accaaggccg aggcggccaa gaccgacgag tccgggaagg
     3901 agcgtcggac cggtcgcgat cggcgggtgc cgaccgaaaa gacccgcatc gtgatcgtgg
     3961 acacccacac cctgtttcgg cgtggggtcc gtaacatcct cgacctggag ccggacatga
     4021 ccgttgtcgg cgaggcgggg agtggccgag aggccatcag cgtggtcgag gagctcacgc
     4081 cggacgtggt cctgatggac ctcaccctgc cggcgccgaa cggcatcgag accacccagc
     4141 gcatcaagcg agagctgccg cacaccggcg tcatcgtgct ggccggcggc gacgacgagg
     4201 accagctgtt cgacgccatc
//
LOCUS       DASYGU010000185         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1367468, whole genome shotgun sequence.
ACCESSION   DASYGU010000185 DASYGU010000000
VERSION     DASYGU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367468"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="miaA"
                     /locus_tag="VGO32_05235"
     CDS             complement(<1..634)
                     /gene="miaA"
                     /locus_tag="VGO32_05235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV7200189.1"
                     /translation="MGPTATGKTDLSLALAGAVPMEILVADSRQVYRGMDIGTAKVGP
                     AARARVPHHLINLVDPDQRFTVADWVELARGLLPDVAARTRLAMVVGGTGLYVTALVD
                     GFNFASQPWSPEKRERLAAELEAEGVQELADRLSRLDPAIAARTDLRNPRRVLRALER
                     AELVGDAGARTVGLDPYPAPLAVLGLVRPWAVLDRRIGERAEQMFASGLLD"
     gene            complement(697..1506)
                     /locus_tag="VGO32_05240"
     CDS             complement(697..1506)
                     /locus_tag="VGO32_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEV7200190.1"
                     /translation="MTDRAATMRCLLIGDIIGKPGRLAIEHALPDLRRELDVDFVIAN
                     GENVAAGAGLTPSLAEGLLESGIDVITSGNHIWDKREIYDYLDTDVPVLRPANYPDDA
                     PGRGWLIARLADGTELAVINLMGRVTMNQLDSPFETADSLLDGMGEPFPALRFVDFHC
                     ELTSEKNAMGWHLDGRVTAVIGTHTHVPTADAKVLPQGTAYLSDVGMTGPRDSIIGFR
                     LDTVLPRFLRHLPTRFEVAEGPVSLNAALVEADRSTGLALSIQLIQRLVEV"
     gene            complement(1503..3035)
                     /gene="rny"
                     /locus_tag="VGO32_05245"
     CDS             complement(1503..3035)
                     /gene="rny"
                     /locus_tag="VGO32_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV7200191.1"
                     /translation="MPETMAIVAIALIAGILGAIIGYVTRRVWSATKIRNAESYADRL
                     VTDARTKQKEIVLEGKDEVLQSQRAAEEEARERRADLQRQERLLLDRSEILDRKSEAL
                     DRREALAEERVAEAEAEKVRLAALRDQQLMSLEQIGGLSATEARHTLIREIEEDAQAE
                     ASQRVRDIERRATEDGEERSRRILTTVMQRMAADHTSEATVTVVHLPTDEMKGRIIGR
                     EGRNIRALEQATGVDLIIDDTPEAVVLSGFDPVRREVARMALTKLIADGRIHPGRIEE
                     TVAKSRQEIEVVMRQAGEQAAYDAGVPGLHAELIRLLGRLKYRTSYGQNVLNHCIETS
                     RLAGMLAAEIGANQNEAKRGGLLHDIGKAVDHEVDGPHAQIGADIAARYGVPPNVCNA
                     IAAHHQEVDQESIEATVVQIADAISASRPGARGEALDGYIKRLDDLQQIALSFPGVER
                     CYAIQAGREVRVLVHPEEIDDAASSRLARDVVRKIEEQLQYPGQIKVTVIRETRAVDY
                     AR"
     gene            complement(3183..>3616)
                     /locus_tag="VGO32_05250"
     CDS             complement(3183..>3616)
                     /locus_tag="VGO32_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7200192.1"
                     /translation="LILGGDGLSRQAALVARFADEFNRSSATPDRLRRSYERVREACV
                     AIGRDPDEVVRSAMIGVLVADTEAELQERVHAQLRMLGSDEGADAWLADRRGRWIMGT
                     PEQAHEQIGALEAAGAQRIMLQDFLPRDLDMIGILGRIAAG"
BASE COUNT          499 a         1259 c         1275 g          583 t
ORIGIN      
        1 cgtccaggag cccggaagcg aacatctgtt ctgcccgctc cccgatccgc cggtcgagga
       61 ccgcccacgg ccggaccagg cccaggacgg cgagcggggc agggtacgga tcaagcccga
      121 ccgtgcgggc cccggcgtcg cccaccagct cggctcgttc cagggcgcga aggacgcggc
      181 gcgggttgcg caggtcggtc cgggccgcga tggccgggtc gaggcgggac aagcgatcgg
      241 cgagctcctg gactccctcc gcctcgagct cggcagccag ccgctctcgc ttctcggggc
      301 tccacggctg ggaggcaaag ttgaagccat ccaccagggc ggtcacgtac agaccggtcc
      361 cgccgaccac catggcgagc cgcgtccgag ccgcaacgtc cggcagcagg ccccgcgcca
      421 gctcgaccca gtcggcaacc gtgaaccgct ggtcggggtc gacgagattg atgaggtggt
      481 gcggcacccg cgcccgtgcc gccgggccga ccttggccgt tccgatgtcc atcccgcggt
      541 acacctggcg cgaatcggcg accaggatct ccatcggcac cgcgccggcg agcgcgagcg
      601 agagatcggt cttgcccgtt gcggtgggac ccaccagggc ggccagcggc ggcgggcggt
      661 cgggacgccg ggctgccgcg agtgtggtca gatcggtcag acctcgacga ggcgctggat
      721 gagctggatg ctgagggcaa ggcccgtgga ccgatcggcc tcgaccaggg ccgcgttcag
      781 gctgaccggc ccctcggcca cttcgaagcg ggtcggcagg tggcgcagga accgtggcag
      841 gacagtgtcc agccggaagc cgatgatcga gtcgcgcggg ccggtcatgc ccacgtcgct
      901 gaggtacgcg gtgccctggg gcaggacctt ggcatcggcc gttgggacgt gggtatgggt
      961 tccgatcacc gcggtcactc gaccgtcgag atgccagccc atcgcgttct tctcggaggt
     1021 gagctcgcag tggaagtcca cgaagcgcag cgccgggaac ggctccccca tcccgtcgag
     1081 caggctgtcg gccgtctcga acggtgagtc gagctggttc atggtcaccc ggcccatcag
     1141 gttgatgacc gcgagctccg tgccatcggc caggcgggcg atgagccagc cccggccggg
     1201 tgcatcatcg ggatagttgg ccgggcggag gacgggcaca tcggtgtcga ggtagtcgta
     1261 gatctcgcgc ttgtcccaga tgtggttgcc gctggtgatg acgtcgattc cggactcgag
     1321 aaggccctcg gccagggacg gcgtcagtcc ggcaccggcg gccacgttct cgccgttggc
     1381 gatgacgaag tccacgtcga gctcacgacg gaggtccggc agcgcgtgct cgatcgcgag
     1441 ccgtcccggt ttgccgatga tgtcgccgat cagcaggcac cgcatcgtcg cggcgcgatc
     1501 ggtcatcgag cgtagtccac ggctcgcgtc tcgcggatca cggtgacctt gatctggccc
     1561 gggtactgga gctgctcctc gatcttgcgc acgacgtcac gggccagccg cgacgaggcc
     1621 gcgtcgtcga tctcctcggg gtggacgagg acccggacct cgcggcctgc ctggatcgcg
     1681 tagcagcgct cgacgccggg aaagctgagc gcaatctgct ggaggtcgtc caggcgcttg
     1741 atgtagccgt ccagggcctc gccccgggcg ccgggccgcg aggcgctgat cgcgtccgcg
     1801 atctggacca cggtggcctc gatcgactcc tggtcgacct cctggtgatg ggcggcgatc
     1861 gcgttgcaca cgttcggcgg cacgccgtag cgggccgcga tgtcggcccc gatctgggca
     1921 tgcgggccgt cgacctcgtg atcgaccgcc ttgccgatgt cgtggagcag cccgccgcgt
     1981 ttggcttcgt tctggttggc gccgatctcg gcggcgagca tgccggccag gcgcgaggtc
     2041 tcgatgcagt ggttcagcac gttctgcccg tagctggtcc ggtacttcag ccgcccgagc
     2101 aggcgaatga gctcggcatg gagtccgggc acgccggcgt cgtacgccgc ctgctccccc
     2161 gcctggcgca tgacgacctc gatctcctgg cgcgacttgg cgaccgtctc ctcgatccgg
     2221 ccaggatgga tccggccgtc ggcgatgagc ttggtgagcg ccatgcgggc cacttcccga
     2281 cggacgggat cgaacccgga caggacgacg gcttccggtg tgtcgtcgat gatcaggtcg
     2341 accccggtgg cctgctccag ggcccgaatg ttccgaccct cgcgaccgat gatccggccc
     2401 ttcatctcgt cggtgggcag gtggacgacc gtgaccgtcg cctcggacgt gtggtcggcc
     2461 gccatccgct gcatgaccgt ggtcaggatc cgtcgcgagc gctcttcgcc gtcttccgtc
     2521 gcgcggcgct cgatgtcgcg cacgcgctga gacgcctcgg cctgggcgtc ctcctcgatc
     2581 tcgcggatga gcgtgtggcg cgcctcggta gcggacagac cgccgatctg ctcgagtgac
     2641 atcagctgct ggtcgcggag ggcggccagc cgcaccttct cggcctccgc ttcggccacg
     2701 cgctcctcgg ccagggcctc gcgtcggtcg agcgcctccg acttgcggtc gaggatctcg
     2761 gagcgatcga gcaggaggcg ctcctggcgc tggagatcgg cgcgccgctc gcgggcctct
     2821 tcctccgccg cgcgctggga ctggaggacc tcgtctttgc cttcgaggac gatctccttc
     2881 tgcttcgtcc gcgcatcggt gaccagccga tccgcatacg actccgcatt ccggatcttg
     2941 gtcgcagacc acacgcggcg cgtcacgtag ccgatgattg cgccgaggat gccggcgatc
     3001 agggcaatgg cgacaatcgc catcgtctca ggcatggctc ctccctctca ggagctgggt
     3061 tttcggtccc ggcgtgcccg gtcgggcccg caaaatgtcg tactcaaacc gatcacggca
     3121 gcgttccgtc gatcggtgcg gggtactcta ctccaactgc ccgggcgggt ccccgcgcgc
     3181 gctcaaccgg ccgcgatccg acccaggatc ccgatcatgt cgaggtcgcg ggggaggaag
     3241 tcctggagca tgatccgctg cgcgccggct gcctcgaggg cgccgatctg ctcgtgagcc
     3301 tgctcgggcg tgcccatgat ccaccggcca cgccggtccg ccagccacgc gtcggcccct
     3361 tcgtcgctgc ccagcatccg cagctgggcg tggacccgtt cctggagctc ggcctccgtg
     3421 tccgcgacga ggaccccgat catcgccgag cgcacgacct cgtccggatc gcggccgatg
     3481 gccacgcacg cctcgcgaac gcgctcgtag ctgcgtcgca gccgatccgg cgtggccgag
     3541 ctgcggttga actcgtccgc gaagcgggcc acgagcgccg cctggcggga cagaccgtcg
     3601 ccgcccagaa tgaggt
//
LOCUS       DASYGU010000186         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_595995, whole genome shotgun sequence.
ACCESSION   DASYGU010000186 DASYGU010000000
VERSION     DASYGU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595995"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGO32_05255"
     CDS             complement(<1..398)
                     /locus_tag="VGO32_05255"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV7200193.1"
                     /translation="MESTRPRPVRRAAAPERIGILGGTFDPPHVGHLWLATLAADALG
                     MAKVFLVPAASPPHKRRRAISHAADRILMTRLAVANDPRLDVSLVEMEREGPSYTVDT
                     LVELKARHADADLVLIMAADSLAQIPSWREP"
     gene            complement(455..1744)
                     /gene="obgE"
                     /locus_tag="VGO32_05260"
     CDS             complement(455..1744)
                     /gene="obgE"
                     /locus_tag="VGO32_05260"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV7200194.1"
                     /translation="MFADRVRIHVTAGSGGDGSPSMRREAHVPRGGPDGGDGGRGGSV
                     IMIAKAGLTSLGDYLRSPHVRATSGGRGARRRAHGRNGTDATVVVPLGTVARRHPEGE
                     LLGEVLAAGDRLVLARGGRGGRGNVHFVSPTHQAPRHYERGDPGEEGWIELELKLIAD
                     IGFVGAPNAGKSTLLAALTAARPEVGSYPFTTTTPNLGVLAIGDGDADPRSVVVADVP
                     GLIEGAHEGQGLGHAFLRHVERCRVLLGVVNGVADDPAGEWRAVAAELGLHDPALLER
                     PMRLAVTKQDLPEAAERWPAVRDALRADGGEPIAVSAHDGSGIDRLLVALAAALEEAD
                     RHEEASPPAPARRLHRFDPTADGWEVLVEADGLRVRGRSIERSAARTDFTNEESRDRF
                     QRRLERMGVDAELRRQGARDGTLVRIGAQELEWGDEE"
BASE COUNT          269 a          687 c          653 g          246 t
ORIGIN      
        1 ggttcacgcc acgacggaat ctgggccagg ctgtccgcgg ccatgatcag gacgagatcg
       61 gcgtcggcgt gacgggcctt cagctcgacc agggtgtcga ccgtgtagga gggaccctcc
      121 cgctccatct cgaccagtga cacgtccagg cgcgggtcgt tggccaccgc cagtcgggtc
      181 atcaagatcc gatccgccgc atggctgatc gcgcggcggc gcttgtgcgg cggcgaggcg
      241 gccggcacca ggaacacctt ggccatcccg agcgcatcgg ctgccagggt ggctagccac
      301 aggtgcccaa cgtggggcgg atcgaacgtg ccgcccagga tcccgatccg ttccggggcg
      361 gcggcccggc ggacgggacg cggccgggtg gactccaccg gccggacgac cgggaccagc
      421 cgcgaggcac cgcgggtcga cggtgggctg gccttcattc ttcgtcgccc cactcgagct
      481 cctgggcgcc aatccgtacc agcgttccgt cgcgcgcgcc ctgccggcgg agctcggcgt
      541 cgacgcccat ccgttcgagc cggcgctgga aacggtccct cgactcctcg ttcgtgaagt
      601 cggtgcgggc ggccgaccgc tcgatcgacc gcccccgcac ccgcagacca tcggcctcga
      661 cgaggacctc ccagccgtcc gcggtggggt cgaaccggtg gagacgacgg gccggcgcgg
      721 gcgggctggc ttcctcgtgc cgatcggcct cctccaacgc cgcggcaagc gcgaccagga
      781 ggcgatcaat cccgctcccg tcatgcgcag agaccgcgat tggttcgccg ccgtccgcgc
      841 ggagcgcatc gcggaccgcg ggccagcgct cggcggcctc gggcaggtcc tgcttggtca
      901 ccgccaggcg catcgggcgc tccaggagcg ccggatcatg gagaccgagc tcggcggcaa
      961 ccgcgcgcca ctccccggcc ggatcgtccg cgaccccgtt caccacgccg agcaggacgc
     1021 ggcaccgttc gacatggcga aggaacgcgt ggcccagacc ctgcccctcg tgggctccct
     1081 cgatcagccc gggaacgtcg gccaccacca ccgatcgagg atccgcgtct ccatcgccga
     1141 tggcaagcac gccaaggttc ggagtcgtgg tcgtgaacgg gtacgagccc acctccggcc
     1201 gggcggcggt cagcgcggca agcagggtgg acttgccggc attcggcgcc ccgacgaagc
     1261 cgatatcggc gatgagcttg agctcgagct cgatccagcc ctcttccccg gggtcaccac
     1321 gctcgtagtg gcgcggtgcc tggtgggtgg gggacacgaa gtgcacgttg ccgcgacctc
     1381 cccgaccgcc tcgggccagc accagccgat ctccggcggc gaggacctcg cccagcagct
     1441 cgccttccgg atggcgccgg gcgaccgttc ccaggggtac cacgaccgtg gcatcggtcc
     1501 cattccggcc gtgggcccgg cggcgcgctc cccgtccgcc gctggtggcc ctgacatgcg
     1561 gcgaccgcag atagtcgccg agcgaggtga ggccggcctt cgcgatcatg atcaccgacc
     1621 cgcctcggcc gccatcaccg ccgtcgggcc ctccgcgcgg gacgtgcgcc tctcgccgca
     1681 tggagggcga gccgtcaccg ccggacccgg ccgtcacgtg gattcgaacg cggtccgcga
     1741 acatcgtggc ccgaccgtga ggccggagcg gctacaggaa gcgcagcggg ttgacgatga
     1801 cgccgttgac gatgaccacg aagtggacgt gaggaccggt gcaccacccg gagca
//
LOCUS       DASYGU010000187         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_442210, whole genome shotgun sequence.
ACCESSION   DASYGU010000187 DASYGU010000000
VERSION     DASYGU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442210"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VGO32_05265"
     CDS             <1..1028
                     /locus_tag="VGO32_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200195.1"
                     /translation="ELQPGVELAYVGGVRGFERRLVGEAGDMPYHQLTVRSLRSAGRS
                     AHTVLDPLRLAAATPQAWQLLRRLEPDAIFSTGGYLAVPLVAAARPRRVPSIVWEGNV
                     VPGRATRAIGRWATRVAVAFPPTADSFGDHAFVSGTPIRSFTGVTRDHARAAMDLGPE
                     DRLMLVFGGSQAVARITAALEDALPELVEDWVVVHLAGEAGMAAAEAARDRLPAAHRE
                     RYRPEAFLTDRMAETLVAADLVLGRAGSSTCAEVAAVGVASILVPYPFAHGHQAANAA
                     WLAHQAAAVAVPDAELNGERLRAEVAALRDDARRASIATAARGLGRPNAARDVAIALI
                     ELAGAAA"
     gene            1031..1993
                     /gene="murB"
                     /locus_tag="VGO32_05270"
     CDS             1031..1993
                     /gene="murB"
                     /locus_tag="VGO32_05270"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV7200196.1"
                     /translation="MVSDSAPQTADALEYFDRLTAERGLAIRRDAVLAPMTTLRVGGS
                     ADRLAEPRTRAELLAVLEAARRAGIACLVIGNGSDLVVADAGVRGLVIRNRAREVALD
                     GDRLSADAGTPMALLVKRATAAGLSGLEWGIAVPGTLGGAVWANAGAHGGEIRDRLAA
                     VETWSPDSGILATMTPAECAFGYRESRFKHDRLVVVGARLTLSADDPTAVGVRVDAFQ
                     AQRAATQPLAEQNAGSVFRNPEGDHAGRLIDAAGLKGLRVGTASVSTRHANFIVTDRD
                     GRAADVRRLADLVRATVRDASGITLEYEIEFVGDWAADLTEGVS"
     gene            1993..>2702
                     /locus_tag="VGO32_05275"
     CDS             1993..>2702
                     /locus_tag="VGO32_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HEV7200197.1"
                     /translation="MARKRIGVFAGGRSAEHEVSIASAEAILRAIDRDRFEPFLVYID
                     AAGGWHLPTGPAPLLGPDDSLARQLGAETITEHSARLEAGDAALPVPLGGAALQAQAA
                     VRTLADAIDVAFLAVHGPFGEDGTLQGFLELAGIPYTGAGVLASAVAMDKVVFKDLMR
                     AHGLPVVAYTSYGRRAWRTAPERVIADALARIGPKSVVKPARLGSSVGMSLAHDPDEL
                     PSAIETAFGWDSKVIVEA"
BASE COUNT          353 a          983 c          980 g          386 t
ORIGIN      
        1 gcgagctgca acccggggtt gagcttgcgt acgtgggtgg tgtccgtggc ttcgagcgcc
       61 ggctggtggg agaggccggc gacatgccct accaccagct gaccgtccgc tcactgcgct
      121 cggccgggcg gagcgcgcac accgtcctgg acccgttgcg cctggcggcg gccacgcccc
      181 aggcctggca gttgctccgt cgcctcgaac cggacgcgat cttctcgacc ggcggctatc
      241 tcgccgtccc gctcgtcgcc gcggccaggc cgcgccgggt cccgagcatc gtgtgggagg
      301 gcaatgtcgt gcccgggcgc gccacccggg cgatcggtcg ctgggccacc cgggtcgccg
      361 tggcgttccc gccgaccgcg gactcgttcg gtgaccatgc cttcgtgtcg ggaaccccga
      421 ttcgcagctt taccggtgta acccgcgacc acgctcgagc agccatggac ctcggccccg
      481 aggaccggct gatgctcgtg ttcggcggct cgcaggccgt ggcacggatc accgctgccc
      541 tcgaggacgc cctcccggag ctggtggagg attgggtcgt cgtccacctc gccggtgagg
      601 caggaatggc agcggctgaa gcggcgcgcg accggctgcc cgctgcccac cgagagcggt
      661 atcgacccga ggccttcctg accgatcgga tggcggagac tctcgtggcc gcggacctcg
      721 tcctcggtcg ggccggatcg tccacctgcg ccgaggtggc cgcggtgggc gtggcttcca
      781 tactcgtgcc gtatccgttc gcgcacggcc accaggccgc caacgcggcg tggctcgccc
      841 accaggcggc cgcggtggcc gtccccgatg ccgagctcaa cggggagcga ctccgggccg
      901 aggtcgccgc gctccgtgac gacgcgcggc gcgcgtccat cgccaccgcg gcgcgcggac
      961 tcggccggcc caacgccgca cgggacgtcg ccatcgcgct cattgagctg gccggggcag
     1021 ccgcgtgagc gtggtgtccg attccgcgcc gcagaccgcc gacgcgctcg agtacttcga
     1081 ccggctgacc gccgaacgtg gcctcgccat ccgccgcgac gccgtccttg cgccgatgac
     1141 gacgttgcga gtcggcgggt ctgccgaccg cctggccgag ccgcgcacgc gcgccgagct
     1201 gctggccgtg ctcgaggctg cgcgcagggc cggcattgcc tgcctggtca tcggcaacgg
     1261 aagcgacctg gtggtggccg atgccggcgt tcggggcctc gtgattcgaa accgcgcccg
     1321 cgaggtcgcc ctcgacggtg accgcctgtc cgccgatgca ggcacgccga tggccctgct
     1381 cgtcaagcgc gcaaccgctg ccgggctctc tggcctggag tgggggatcg cggtcccggg
     1441 aaccctcggc ggcgccgtgt gggcaaacgc cggcgcccac ggcggggaga tccgcgaccg
     1501 cctggcggcc gttgaaacct ggtcgccgga cagcggcatc ctggcgacca tgacccccgc
     1561 cgaatgcgcc tttggatacc gcgagtcgcg gttcaagcac gaccggctcg tggtcgtggg
     1621 agcccgcctg acgctctcgg ctgacgaccc cacggcggtg ggcgtacggg tcgacgcctt
     1681 ccaggcccag cgggccgcca cccagcccct tgccgagcag aacgcgggca gcgtcttccg
     1741 caaccccgag ggagatcacg cgggccgcct gatcgacgcg gctggcctga aggggttgcg
     1801 ggtcgggacc gcctcggtca gcacccgcca cgcgaacttc atcgtcaccg accgcgacgg
     1861 gcgggcggcc gacgttcggc gcctggccga cctggtccgc gccacggtac gggacgcgag
     1921 tgggatcacc ctcgagtacg agatcgagtt cgtcggcgac tgggccgcgg acctgacgga
     1981 gggggtgtcc tgatggcccg aaagcggatc ggcgtgttcg ccggtggccg gtcggccgag
     2041 cacgaggtca gcatcgcgtc ggccgaggcg atcctgcggg ccatcgaccg cgatcggttc
     2101 gagcccttcc tcgtctacat cgacgcggcc ggtggctggc acctgccgac gggaccggct
     2161 ccgcttcttg gcccggacga cagcctggcc cgccagctcg gggcggaaac gatcaccgag
     2221 cacagcgccc ggctggaagc cggggacgcc gcgctgcccg tgccgctcgg cggtgccgcg
     2281 cttcaagccc aggccgcggt ccgcaccctg gcggacgcaa tcgacgtcgc gttcctggcc
     2341 gttcacggcc cattcggcga ggacggcacg ctccagggat ttctcgagct ggccgggatc
     2401 ccgtacacgg gggccggcgt gctggccagt gcggtggcaa tggacaaggt cgtattcaag
     2461 gacctcatgc gcgcccatgg cctcccggtc gtcgcctaca cgtcgtacgg ccgccgggcc
     2521 tggcggacgg cacccgaacg agtgatcgcc gatgcgctgg cccgaatcgg gccgaaatcg
     2581 gtggtcaaac ccgcacgtct cggcagcagc gtggggatga gcctcgccca cgatcccgac
     2641 gagctgccat cggcgatcga gaccgcgttc ggctgggact cgaaggtgat cgtggaggcg
     2701 ta
//
LOCUS       DASYGU010000188         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1213838, whole genome shotgun sequence.
ACCESSION   DASYGU010000188 DASYGU010000000
VERSION     DASYGU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213838"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGO32_05280"
     CDS             <1..213
                     /locus_tag="VGO32_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200198.1"
                     /translation="IDTAAIRPEFGDAGLGPVVGFVRERATDARIAVRPGPGADWLVG
                     AEAGVGVVILSGDGAACAEAVAALGL"
     gene            complement(229..648)
                     /gene="sodX"
                     /locus_tag="VGO32_05285"
     CDS             complement(229..648)
                     /gene="sodX"
                     /locus_tag="VGO32_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018807665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HEV7200199.1"
                     /translation="MRIAWLVAGAALGYLGWQTLRHLDAVEVSGRSMSPTLEPGDRLL
                     VETWSLGRRTPRVGDVVIAPDPREPSRELVKRVAAVGDGRVALRGDSAKSTDSRRFGT
                     VPLTDVRARVAVRYWPLAKAGPIPTVDLPLGGEIAPG"
     gene            complement(663..1082)
                     /gene="sodN"
                     /locus_tag="VGO32_05290"
     CDS             complement(663..1082)
                     /gene="sodN"
                     /locus_tag="VGO32_05290"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEV7200200.1"
                     /translation="MLTLRRLIHPTRQVAAHCDLPCGIYDPDQARIEAESCLRILEKY
                     AASDDPHFKARAIDIKEERADLVIGHLDCLWHDYFKPEHLEQFPELHDTFWKAAKQAS
                     KVKGSLDVEEAKTLLVMIDKVGDMWKATGGPEKTRVH"
     gene            complement(1151..>1722)
                     /locus_tag="VGO32_05295"
     CDS             complement(1151..>1722)
                     /locus_tag="VGO32_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEV7200201.1"
                     /translation="EVLIGLRWVHFVAGITWIGLLYWFNLVNVPYQRVLDPALKGQVN
                     PPLFRRALAWFRWSAVVTVGAGLLLIYGLYWQDGDLFGSNTEKTIFAGGLLGIVMLAN
                     VWMFIWPNQRRVLAAIDAGEAPNPAWPRRALIASRVNFALSFPMLFFMAGSAHYPLDW
                     PGILIVGVVLAAIGTLVVLTVQKYWATRF"
BASE COUNT          270 a          580 c          613 g          259 t
ORIGIN      
        1 atcgacaccg ccgcgattcg gcccgagttc ggcgacgccg gcctgggtcc ggtcgtcggc
       61 ttcgtccgcg agcgcgcgac cgacgcgcgg atcgcggtgc gcccgggacc gggcgcggac
      121 tggctcgtcg gcgcggaggc cggtgtcggc gtcgtgatcc tgtcgggcga cggcgcggcg
      181 tgcgcggagg cggtcgccgc tctcggtctc tgaacgcgtc ctggagcgtc agcccggcgc
      241 gatctcaccg cccaggggca ggtccaccgt cgggatcggg ccggccttgg ccaggggcca
      301 gtaccggacc gcaacccggg ctcggacgtc cgtcagcgga accgtgccga accgccgtga
      361 atcggtgctc ttggccgagt cgccgcgaag ggccacccgc ccatccccga ccgcggcgac
      421 gcgcttcacg agctcgcggc tcggctcgcg cgggtcgggc gcaatgacca cgtcgcccac
      481 ccggggcgtt cggcgtccca acgaccaggt ctcgaccagc agccggtccc cgggctcgag
      541 cgtggggctc atcgagcgac cgctcacctc gaccgcgtcg aggtgccgga gcgtctgcca
      601 gcccaggtag ccgagggcgg cacccgccac caaccaggcg atcctcatcg gttcgagccc
      661 ggtcagtgga cccgggtctt ctccggtccg ccggtggcct tccacatgtc gccgaccttg
      721 tcgatcatga ccaggagcgt cttcgcctcc tcgacgtcga gcgagccctt caccttggat
      781 gcctgcttgg cagccttcca gaacgtgtcg tgcagctcgg ggaactgctc gaggtgctcg
      841 ggcttgaagt agtcgtgcca caggcagtcg aggtgcccga tcacgaggtc ggcgcgctct
      901 tccttgatgt cgatcgcgcg ggccttgaag tgggggtcgt cgctggccgc gtacttctcc
      961 aggatccgca ggcaggattc ggcttcgatc cgtgcctggt ccgggtcata gatgccacac
     1021 ggcaggtcgc agtgggccgc cacctggcgg gtgggatgga tgagtcgtcg tagtgtcagc
     1081 atgagccgat tgtgcccgac cggggcaatt gaggcactgc cggccgagcg agggagattt
     1141 cagattcgcg tcagaagcgc gtggcccagt acttctgcac ggtgaggacg acgagcgtgc
     1201 caatggctgc caggacgacg cccacgatca ggatccccgg ccagtcgagc gggtagtggg
     1261 ccgagccggc catgaagaac agcatcggga acgacagggc gaaattcacg cgcgatgcga
     1321 tcagcgcccg ccgtggccac gcggggttcg gcgcctcacc ggcgtcgatc gcggccagga
     1381 cccggcgctg gttgggccag atgaacatcc acacgttggc cagcatgacg atcccgagta
     1441 ggccgccggc gaagatcgtc ttctcggtgt tcgacccgaa cagatcgccg tcctgccagt
     1501 acaggccgta gatgagcagg aggccggcgc cgacggtgac gaccgccgac cagcggaacc
     1561 aggcgagcgc ccggcggaag agcggcgggt tgacctgacc cttgagcgcg gggtccagga
     1621 ctcgctggta cggaacgttg accaggttga accagtacag caggccgatc caggtgatgc
     1681 cggccacgaa atggacccag cgcagaccga tgagaacctc cg
//
LOCUS       DASYGU010000189         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1316471, whole genome shotgun sequence.
ACCESSION   DASYGU010000189 DASYGU010000000
VERSION     DASYGU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316471"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGO32_05300"
     CDS             <1..677
                     /locus_tag="VGO32_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV7200202.1"
                     /translation="DRLATLAAEKNVSLVVGTLEPEGREVFNTALIFSPDGIRHRYRK
                     MHLPFLGVDRFATPGPDAPEVIDLAGMRVGVLICYDLRFPEAARVCALDGADLIALPT
                     NWPVGVDFHPGIFAPARAAENHVYLLACDRVGEERGTTFIGRSILLDTNGKELARASD
                     TEEETIVGEVDWELARQTHHRKIPGEHEWDTIGDRRPGLYGRLLEPARDRLHPPSADL
                     ISGDVE"
     gene            complement(751..963)
                     /locus_tag="VGO32_05305"
     CDS             complement(751..963)
                     /locus_tag="VGO32_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200203.1"
                     /translation="MPLSQWPRSRRVILAVVTSALWLGYMVIGLAVINTLFPTIEGQG
                     PSGTAAFIGLLSGFFVTGIIMWAFSD"
     gene            complement(989..1840)
                     /locus_tag="VGO32_05310"
     CDS             complement(989..1840)
                     /locus_tag="VGO32_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV7200204.1"
                     /translation="MNPDELVGFLESNWFALVFWSLASLLVFRLVRPVVDRIGERIIR
                     TREAQGIDPAELNELRKRLSTIEDLVSKLLRAAVIFVILVVLLTLLDLVSVVVALGVV
                     FAGLAIAGQSIVLDYLMGILILVEGQFYVGDWITIATGNVTVSGTVEEIGLRRTILRD
                     VSGTVHSVSNGEVRVSSNMTRVYAVLQVEIPLIPEVDLEQAIAVTNRVGAEMYADPEW
                     SSRLLEAPRYGSIPNVTDLGVTLRAIGRVQGADRFAAASELRRRLLVAYAGDSIAVAQ
                     RIRPPAP"
     gene            complement(1845..>2908)
                     /locus_tag="VGO32_05315"
     CDS             complement(1845..>2908)
                     /locus_tag="VGO32_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7200205.1"
                     /translation="PDDTYWCMADIGWVTGHSYIVYGPLANATTGVIYEGAIDFPDKD
                     RWAQIAARLGVTILYTAPTAIRALMKYGADVFASDDLSKLRLLGSVGEPINPEAWAWY
                     WQHIGGGRCPVVDTWWQTETGMILITPLPGITTLKPGSATRPFPGIKADVVNEKGESV
                     PLGGGGYLVLERPWPAMLRGIYGDPERYRQTYWSRFPGRYFAGDGCKRDTEGYYWLLG
                     RVDDVMNVSGHRISTTEVESALVSHPSVAEAAVVGRSDPITGQAIFSYVILRAGHEPS
                     EELANALREHVAEVIGKLARPKQVMFTPDLPKTRSGKIMRRLLRDIAEQRDLGDVTTL
                     ADAGIVSTIREQSGAGEDE"
BASE COUNT          473 a         1004 c         1002 g          429 t
ORIGIN      
        1 aggaccgcct cgcgaccctg gccgccgaaa agaacgtatc gctggtggtg ggtaccctcg
       61 agcccgaggg ccgcgaggtc ttcaacaccg ccctcatctt ctcgcccgac ggcattcgac
      121 atcggtaccg gaagatgcac ctcccgttcc tcggcgtgga ccggttcgcg accccgggcc
      181 ccgacgcgcc ggaggtcatt gacctcgccg ggatgcgggt cggcgtgctc atctgctacg
      241 acctgcgctt ccccgaggcg gcccgcgtct gcgccctgga cggggctgac ctgatcgccc
      301 tgcccaccaa ctggccggtc ggcgtcgact tccacccggg gatctttgcc cccgcccggg
      361 ccgcggagaa ccacgtctac ctgctcgcct gcgatcgagt gggcgaggag cgcgggacca
      421 cgttcatcgg ccggtcgatc ctgctggaca ccaacggcaa ggagctggcg cgggcgagcg
      481 acaccgagga ggaaacgatc gtgggcgagg tggactggga gctcgcgcgc cagacgcatc
      541 accgcaagat ccccggcgag cacgagtggg acacgatcgg cgatcggcgg cccggactgt
      601 acgggcgtct gctcgagccg gcccgcgatc ggctgcatcc gccgagcgcg gacctgatct
      661 cgggggacgt cgagtagctc gagccgcatc cgcggccacc cggttctcat gggcaggaaa
      721 cgcgccggac ggtgccggcg tgaggtcagg tcagtcggag aaggcccaca tgatgatgcc
      781 ggtcacgaag aagccgctga ggagcccgat gaacgccgcc gtccccgacg ggccctggcc
      841 ctcgatggtc gggaacaggg tgttgatcac cgccaggccg atgaccatgt agcccagcca
      901 cagggcgctg gtcaccaccg ccaggatcac gcgccgggat cggggccact ggctgagagg
      961 catcatgcac gggccatcgt aacggctctc acggcgctgg tgggcggatc cgttgggcca
     1021 ccgcgatgct gtccccggca tacgccacca gcaggcgccg ccggagctcg gaggcggcgg
     1081 cgaaccggtc cgcaccctgg acccgtccga tggcacggag cgtgaccccc aggtcggtga
     1141 cattcgggat cgagccgtag cgaggagcct ccagcagccg gctggaccac tccggatcgg
     1201 cgtacatctc ggcacccacc cggttggtga ccgcgatcgc ctgctcgagg tcgacctcgg
     1261 ggatgagcgg gatctcgacc tgcaggaccg cgtacacccg ggtcatgttg gacgagaccc
     1321 ggacctcgcc gttggacacc gaatggaccg tgcccgacac gtcacggagg atcgtccgcc
     1381 gcagcccgat ctcctccacc gtgcccgaga cggtcacgtt cccggtggcg atcgtgatcc
     1441 agtccccgac gtagaactgg ccctcgacca ggatcaggat gcccatcagg tagtccagga
     1501 cgatggactg gcccgcgatg gccagcccgg cgaacaccac gcccagggcc actacgaccg
     1561 acacgaggtc gagcagggtc aggagcacga ccaggatcac gaagatcacg gccgcccgaa
     1621 gcagcttcga gacgaggtct tcgatggtgg acagccgctt gcggagctcg ttcagctcgg
     1681 cggggtcgat gccctgcgcc tcgcgagtgc ggatgatccg ctcgccgatc cggtcgacga
     1741 ccgggcggac caggcggaag acgaggagcg aggcgaggct ccagaagacg agggcgaacc
     1801 agttcgactc caggaaaccg acgagctcgt cggggttcat cggttcattc gtcctcgccg
     1861 gcgcccgact gctcgcgaat ggtggacacg atcccggcgt cggcgagggt cgtcacgtca
     1921 cccaggtcac gctgctcggc gatgtcgcgc agcaggcgac gcatgatctt gccgctccgg
     1981 gtcttgggca ggtccggggt gaacatcacc tgcttggggc gcgcgagctt gccgatgacc
     2041 tcggcgacgt gctcgcgcag cgcgttcgcc agctcctccg acggctcgtg gcccgcgcgc
     2101 aggatcacgt acgagaagat cgcctggccg gtgatcgggt cgctccgacc cacgaccgcg
     2161 gcctcggcca ccgacgggtg ggagacgagg gccgattcga cctcggtggt gctgatccgg
     2221 tggccgctga cgttcatcac gtcgtccacc cggccgagga gccagtaata gccctcggta
     2281 tcgcgcttac agccgtcacc ggcaaagtag cggcccggga agcggctcca gtaggtctgc
     2341 cggtagcgct cggggtcgcc gtagatccca cgcagcatgg ccggccacgg acgctccagg
     2401 acgaggtagc cgcccccgcc cagcgggacg ctctccccct tctcgttgac cacgtcggcc
     2461 ttgatgccgg ggaacggccg ggtggcgctc cccggcttga gggtcgtgat ccccggcagc
     2521 ggcgtgatca ggatcatccc ggtctcggtc tgccaccagg tgtcgaccac cgggcaccgg
     2581 cccccgccga tgtgctgcca gtaccaggcc catgcctctg ggttgatcgg ctcgcccacg
     2641 ctgcccagca ggcggagctt cgagaggtcg tcgctggcga agacatcggc gccgtacttc
     2701 atgagtgcgc ggatggcagt cggggcggtg tataggatcg tgacccccag ccgggccgcg
     2761 atctgggccc atcggtcctt gtccgggaag tcgatcgcac cctcgtagat gacaccggtc
     2821 gtggcgttcg cgagcggccc gtagacgatg tacgaatgcc cggtcaccca gccgatgtcg
     2881 gccatgcacc agtacgtgtc gtcgggct
//
LOCUS       DASYGU010000190         5914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1677176, whole genome shotgun sequence.
ACCESSION   DASYGU010000190 DASYGU010000000
VERSION     DASYGU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677176"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGO32_05320"
     CDS             <1..533
                     /locus_tag="VGO32_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200206.1"
                     /translation="IVGLTLVFLGVYVFVMLYQYARHGGEFRLRSRWMLVFAGFQNAW
                     RRIQARIHGHDHVDPVEAASYGPRTAFGVGMIHGVGAETASQALLIAAVGGAASAGLG
                     VPMLFAFVIGLVFSNTVVVILSATGYIASQARTRIYLAVGILTGIFSLWIGFLFLFQF
                     DAALPSLDDVFHFIGG"
     gene            538..990
                     /locus_tag="VGO32_05325"
     CDS             538..990
                     /locus_tag="VGO32_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEV7200207.1"
                     /translation="MTDSARASLPEPWASVGGHLRQRGLRWTPQRRQVIEVLAASEGH
                     VTGAEIVERCRRLDPATVPSTVYRTLDVLEELGFIRHGHGANGQEEYHVQPTTDHGHL
                     HCASCGRQWELNNDDASAFVARIERRRGFQIDLSHLTLVGRCPDCSTI"
     gene            1006..1473
                     /locus_tag="VGO32_05330"
     CDS             1006..1473
                     /locus_tag="VGO32_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200208.1"
                     /translation="MSSQPASVRFGHVSVVARDWRALADYYIAVFGCVVVPPERDYSG
                     EDLARGTGVPGAALRGAHLRLPGLGDDGPTLEIYQYAEPADRLPTAANRLGLAHTAFV
                     VPDVASARDAALTAGGRAVGEIVTLTTATGRRVTWCYVTDPEDNIVELQAWSD"
     gene            1677..3338
                     /locus_tag="VGO32_05335"
     CDS             1677..3338
                     /locus_tag="VGO32_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEV7200209.1"
                     /translation="MVSLSRRRSTRFHALATLGLALAAVSAGLLPTASLAGQPVYQRP
                     LDVVFIIDRSGSMDQVTPDPGAPIAPGVNNTRLGWANDAANALVSALESAGGVGAGGL
                     HQVGLVTYGGGTSTIDLPLGVSAPGAVHAAIDVWDGLDGDGNTPLKEGMASAVATMTA
                     GGRTEMDGVAVLRVFILLSDGRPNPDPGSRPGEADVAAYLGAVDQAFGIGIGATGGAD
                     PGLEPDLALMQAVSSPVTNFSHVVDAASLPNLFASMTDELVYGDIQISSSAAPGGPVD
                     PGTPVTFTYVVWNNSEDTPLHEVSVADAVCSAMSGPAMAGGNVDAHLSSGETWTYTCT
                     ASLEATAASDACATGHFIAGGSDESCAALTVQVVAPATPSPTPTANPAPTGPPTPSPT
                     VNPAPTGPPSPTPTPFQAPAAPAATPTPSAAVAVVEPSPSPSEMPAEIASPTPTPEPP
                     RTAALEPPLLVPPAPPAAGGSPSQPTDVVGGILAAALQQAGAVVQPQAAVAVATAFSF
                     PLGLMVAVMGFLVGQGRIDARDPKLRQAPRTPKDTVMNFRNEEEL"
     gene            3335..5854
                     /locus_tag="VGO32_05340"
     CDS             3335..5854
                     /locus_tag="VGO32_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7200210.1"
                     /translation="MNTRTEMTDYAALSERMSNLLLLRLALAAVVLISAAMRPEALGV
                     AFPVLAGVTVGYLFAAITFEAVRRRTGRAGFTILNSLLLLDGLYLAFAMYATGGTHSP
                     MRFLIYLHLVAVSLLASYRTGLKIALWDSLLLFVMLYAQAAGLVPPVDTAAGAAIVFD
                     SMPVLNVTAFWLFALATSIFSAMNERELRNRRADLEALVDLGTGLDAVGDPVHQARIV
                     LDGLADRFETKRGVVLGASDDRIVILAGKGVADVQSAPTAVDAVVQKAWDRHDVLPIR
                     RIDVDKNPLLAAALPNARNLLIAPMIAEGLPVGAIVIEHRSRSVFGVERRVMSVLAQF
                     ASVAALNFRNAVLLRHVQDLAERDSLTGAANRRTFQHALERIIAAPRRGSAKTTGVLF
                     LDVDDFKVVNDTLGHAAGDALLVAVTERISALVRQGDLVARLGGDEFAILTSDDNDLH
                     RSRAMAERLVRELRTPYLIRDEQVAVTASIGIASAHGHDQSAEEVLRNADVAMYMAKA
                     NGKARFAIFDPGMHAAIRERHELGAQLQRAVELEQLRLVYQPIVELGTGRVAGLEALV
                     RWQHPERGLIAPGEFIEIAEENGAILPIGSWVLREACQHAGNWQREGLTPKGIFLCVN
                     VSAREVQQPDFVASVRETLTEAGLEPSSLMMEITETALLKATGPTMSTLTELRELGVQ
                     VVIDDFGTGYFSLSHLRQFPVDALKIASEFVQVSNTDARSAALAGAIVALSQSLGITT
                     VAEGIETGEQAKRMESLGCAYGQGFFFASPMEQAEIDKGVAGLANAVRRQRRRRTDTA
                     PAADTGTTTTTTTPTPAARGTRAPRIRTRTPRLIPADPTAA"
BASE COUNT          867 a         2180 c         1939 g          928 t
ORIGIN      
        1 ggatcgtcgg gctcaccctc gtcttcctgg gcgtctacgt gttcgtgatg ctgtaccagt
       61 acgcgcgcca cggcggcgag ttccggctgc gcagccggtg gatgctcgtg ttcgccggat
      121 tccagaatgc ctggcggcgg atccaggcca ggatccacgg ccatgaccac gtcgacccgg
      181 tcgaggccgc ctcctacggc ccgaggaccg cgttcggggt cgggatgatc catggcgtcg
      241 gcgccgaaac ggcgtcccag gctctcctca tcgctgcggt cggcggggcg gccagcgccg
      301 ggctcggcgt gccgatgctg tttgcgttcg tcatcggtct ggtcttctcg aataccgtcg
      361 tcgtcatcct gagcgcgacg ggctacatcg ccagccaggc tcggacccgg atctacctgg
      421 ccgtggggat cctgaccggc atcttcagcc tgtggatcgg cttcctgttc ctgttccaat
      481 tcgatgccgc gttgccgagt cttgacgatg tcttccattt catcggaggc tgatccgatg
      541 accgatagcg cacgggcctc tctgcccgaa ccgtgggcct cggtcggcgg ccatctgcgc
      601 cagcgcgggc tccgctggac cccgcagcgg cggcaggtca tcgaagtcct cgccgcgagc
      661 gagggacatg tcacgggggc cgaaatcgtc gagcgctgcc gtcgtctcga tcccgccacc
      721 gtgccgtcca ccgtctaccg cacgctcgat gtcctcgagg agctgggctt catccgtcac
      781 ggccatggcg ccaacggcca ggaggagtat cacgtccagc cgacgaccga tcacggccac
      841 ctgcactgcg cctcctgcgg tcggcaatgg gagctgaaca acgacgatgc gtcggccttt
      901 gtcgcccgga tcgagcgtcg gcgtgggttc cagatcgatc tgtcccatct gacgctggtg
      961 ggacgatgcc cggactgctc aacgatctaa cggacggagg tggccatgag ctcgcaaccg
     1021 gcaagcgtcc gcttcggcca tgtcagcgtg gttgcccgcg actggcgtgc gctggccgac
     1081 tactacatcg ccgtgttcgg ctgcgtcgtc gtgccgcccg agcgtgacta cagcggtgag
     1141 gacctggccc ggggtaccgg cgttccgggt gccgccctgc gaggcgcgca ccttcgcctg
     1201 cccggtctcg gtgacgacgg accgacgctc gagatctacc aatacgccga gcctgccgac
     1261 cgcctgccca cggccgccaa ccggcttggc ctggcccata ccgccttcgt cgtaccggac
     1321 gttgccagtg cccgagacgc ggccctgacg gccgggggac gggccgtggg cgagatcgtg
     1381 accctcacca ccgccacggg tcggcgggtg acctggtgct acgtcaccga tccggaggac
     1441 aacatcgtcg agctccaagc ctggagcgac tgacccgggc acggttcgcc gaggacgggg
     1501 agtactccgg tccggctcga gtactgcatc agaccccact tcgggtgttt tcgtgttttt
     1561 gcccccggtg acgaatcgca ccggggcgta ccccggtgac gatcacgcca tagccggtat
     1621 ggccgggcgt tcgcatcctt cggcgtgtca tggatacagg ccggttgggt caaccaatgg
     1681 tcagcctgag tcgacgccgc tcgacgcgct tccacgcgct cgcgacgctt ggcctggcac
     1741 tggccgcggt ctcggccggt ctgctgccca ccgcgagcct ggccggacag ccggtctacc
     1801 agcgccccct ggacgtcgtc ttcatcatcg accggtcagg ttcgatggac caggtcacgc
     1861 ccgatcccgg cgctccgatc gcgcccggcg tcaacaacac acgcctcggc tgggcgaacg
     1921 atgccgccaa cgccctggta agcgcgctcg agagtgcggg aggcgtcggc gccggcggat
     1981 tgcaccaggt gggcctcgtc acctatggcg gtggcacctc gacgattgac ctgccgctgg
     2041 gcgtatccgc gcccggcgcc gtccatgccg ccatcgacgt ctgggatggg ctcgacgggg
     2101 acggcaatac gcccctgaag gaagggatgg cctccgcggt ggcgaccatg acagccggcg
     2161 gccggaccga aatggacggc gtcgcggtat tgcgggtctt catcctgctg tccgatggtc
     2221 gaccgaaccc ggaccccgga tcccgccccg gcgaggcaga cgtcgccgcc tacctcggcg
     2281 cggtggacca ggcgtttggc atcggcatcg gcgcgaccgg cggagccgac ccaggactcg
     2341 agcccgatct ggccctgatg caggccgtgt ccagcccggt caccaacttc agccatgtcg
     2401 tcgacgcagc cagtctgccc aacctgttcg cgagcatgac cgacgagctg gtgtacggcg
     2461 acatccagat ctccagcagc gcggcaccag gcggcccggt cgaccccggg acccccgtga
     2521 cattcacgta cgtcgtgtgg aacaacagcg aggacacccc gttgcacgag gtttccgttg
     2581 ctgacgcggt ctgcagcgcg atgagcgggc cggccatggc cggcggcaat gtggacgcgc
     2641 acctctcctc cggcgaaacc tggacctaca cctgcaccgc gtcactggag gcgaccgccg
     2701 ccagcgatgc ctgcgcgaca ggccacttca tcgccggcgg cagcgacgag tcgtgtgcgg
     2761 cgctcactgt ccaggtcgtg gccccggcga cgccgtcgcc cacgccgacc gcgaacccgg
     2821 cgcctaccgg tccgcccacg cccagcccga ccgtgaaccc ggcgccaacc ggtccgccgt
     2881 cgcccacccc gacgccattc caggcgccgg ccgccccggc cgccacgccc actccttccg
     2941 ccgcggtggc cgttgtcgag ccatccccct cgccgagcga gatgccggcc gagatcgcca
     3001 gccccacgcc gacgcctgag ccgccacgaa ccgccgcgct cgagcccccg ctgctggtgc
     3061 cccctgcccc gccagcagct ggcggttcgc cctctcagcc gacggacgtg gtcggaggca
     3121 tcctcgccgc cgccctccag caggccggtg ccgtcgtgca gccccaggcc gcggttgcgg
     3181 tcgcgaccgc gttcagcttc ccgctgggcc tgatggtggc ggtgatgggc ttcctcgtcg
     3241 gtcagggccg gatcgacgca cgcgatccca agcttcgcca ggcgccacgg acaccgaagg
     3301 acacggtcat gaacttccgg aacgaggagg agctgtgaac acccgcaccg agatgaccga
     3361 ctacgcggcg ctcagcgagc ggatgagcaa cctgctcctc ctgcgcctcg cgctcgccgc
     3421 cgtcgttctg atctcggccg ccatgcggcc ggaggcactg ggggtcgcct tcccggtcct
     3481 ggccggggtc acggtcggat acctcttcgc cgccatcacc ttcgaggccg tgcgccgccg
     3541 gaccggtcgc gccggtttca cgatcctgaa cagcctcctc ctgctcgacg ggctgtacct
     3601 cgccttcgcg atgtacgcca ccggcggcac ccacagcccg atgcgattcc tcatctacct
     3661 gcacctggtc gccgtatccc tgctcgcctc gtaccggacc ggcctgaaga tcgcgctgtg
     3721 ggactcgctc ctgctgttcg taatgctcta tgcccaggcc gccggtctcg tcccgccggt
     3781 cgacaccgcc gccggggcgg cgatcgtgtt cgacagcatg ccggtgctga acgtgaccgc
     3841 gttctggctc ttcgcgctgg cgacctcgat cttctctgcc atgaacgagc gggagctccg
     3901 caaccgccgc gccgacctcg aggccctcgt cgacctcggc acggggcttg acgccgtcgg
     3961 cgatccggtc caccaggccc gcatcgtgct ggacgggctg gccgatcggt tcgagaccaa
     4021 gcgcggggtg gtcctgggcg cctccgacga ccggatcgtg atcctcgccg ggaagggcgt
     4081 ggccgacgtc cagagcgcgc cgaccgcggt cgacgcggtg gtgcagaagg cctgggaccg
     4141 acatgacgtg ctgccgatcc gtcggattga cgttgacaag aacccgctgc tggccgccgc
     4201 cctgcccaac gcccgcaacc tgctgatcgc ccccatgatc gccgagggcc tgccggtcgg
     4261 ggcaatcgtg atcgagcatc ggtctcgcag cgttttcggt gtcgagcggc gcgtgatgtc
     4321 ggtgctcgcg cagttcgcct ccgtcgcggc cctgaacttc cgcaacgcgg tgctgctgcg
     4381 gcacgtccag gatctcgccg agcgcgactc gttgaccggt gccgccaacc gtcggacctt
     4441 ccagcatgcg ctggagcgga tcatcgccgc cccgcgccgc gggtcggcca agacgaccgg
     4501 ggtgctgttc ctcgacgtgg acgacttcaa ggtggtcaac gatacgctgg gtcacgcggc
     4561 aggcgacgca ctgctcgtcg ccgtcaccga acgaatcagc gccctcgtcc gtcaaggcga
     4621 cctggttgcc cgtctcggcg gcgacgagtt cgcgatcctg acctcggatg acaacgacct
     4681 gcaccggtcg cgggccatgg ccgagcgcct ggttcgagag ctgcggaccc cgtacctgat
     4741 ccgcgacgag caggtggccg tcaccgccag catcggcatc gccagcgcgc atggccacga
     4801 ccagagcgcg gaggaggtcc tccgcaacgc ggacgtcgcg atgtacatgg ccaaggccaa
     4861 cggcaaggcg cgcttcgcga tcttcgaccc cggcatgcac gccgccattc gcgagcgcca
     4921 cgagctcgga gcgcagctcc agcgcgcggt cgagctcgag cagcttcgcc tcgtctacca
     4981 gccgatcgtc gagctgggga ccggccgcgt ggccggcctg gaggccctgg tccgctggca
     5041 gcaccccgag cgtggcctga tcgcgcccgg cgagttcatc gagatcgccg aggaaaacgg
     5101 cgccatcctg cccatcggca gctgggtgct ccgcgaagcc tgccagcacg cggggaactg
     5161 gcagcgcgag ggtctgaccc ccaagggcat cttcctctgc gtgaacgtct ctgcccgcga
     5221 ggtgcagcag cctgacttcg tggcctcggt ccgcgaaacc ctgaccgaag ccggcctcga
     5281 gccgtcgagc ctaatgatgg agatcaccga gacggccctg ctgaaggcga ctgggccaac
     5341 catgtccacc ctgaccgagc tccgcgagct cggcgtccag gtcgtgatcg acgacttcgg
     5401 gaccggctac ttctcgctca gccatctccg gcagttcccg gtcgatgcgc tgaagatcgc
     5461 gagcgagttc gtccaggtct cgaacacgga cgcccgctcg gcggccctgg ccggggccat
     5521 cgtcgccctc agccaatcgc tcggcatcac gaccgtggcc gaaggcatcg agaccggcga
     5581 gcaggccaag cggatggagt cgctcggctg cgcgtatggc cagggcttct tcttcgcgtc
     5641 cccgatggag caggccgaga tcgacaaggg cgttgccgga ctggcgaacg cggttcgccg
     5701 gcagcgccgt cgccggacgg acaccgcgcc ggccgctgac acaggcacga ccacgaccac
     5761 gaccacgccc acacccgcgg cacgcggcac ccgcgctccg cggattcgaa cccgaacccc
     5821 acgcctcatc cccgcagacc cgaccgcggc ctgacccccc gcggccgggt cacttagcag
     5881 gtgccagccg gcgacgctcc tccaaccggc tggc
//
LOCUS       DASYGU010000191         7752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_391633, whole genome shotgun sequence.
ACCESSION   DASYGU010000191 DASYGU010000000
VERSION     DASYGU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391633"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /gene="glgP"
                     /locus_tag="VGO32_05345"
     CDS             <1..1741
                     /gene="glgP"
                     /locus_tag="VGO32_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV7200211.1"
                     /translation="SLPVYSGGLGVLAGDLLKAASDARLPMIGVGPFYHRGYFRQRID
                     AEGRQEHLEPDVDPRDLPLRRASSAEGSPLEIRVELPGRDVHLAIWVAQVGRVPLLLL
                     DADVEGNEPEDRRVTDELYVAARRTRLTQEVLLGVGAARALDALGIRPAVWHLNEGHS
                     ALVVLERARLAGVADPTLDGAARLARAGRDMVMTLHTPVPAGNECYDPAVADELLAPA
                     ASAIDLSSDRLHALGKGPEGTNGVFDLTAFGLRQAAAINAVSQLHGRTATATWRSAIG
                     RDVISVTNGVHLATWQAQPMQAALRAAGEAAIGDRELWDAHAEQKRQMIDFLGGRLTR
                     QAIRHGVAPEAVGDLAGALDPHALTMGFARRFATYKRADLILHDMKRLAHLLADPDRP
                     VQLIFAGKAHPADEAGRAVLGHVVDASRRVGLAGRIFFIEDYDLRVARFLVAGVDVWL
                     NTPRRPLEASGTSGMKAALNGVPSVSILDGWWDEAFDGTNGWAVGRPSSEDGGTDEED
                     AESLYRILETEVVPAFFGRSDVGIPEAWIGRMRGALTTGLARFTADRVLRDYVEDLYR
                     PVAGMDGEVARSA"
     gene            complement(1738..3054)
                     /locus_tag="VGO32_05350"
     CDS             complement(1738..3054)
                     /locus_tag="VGO32_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV7200212.1"
                     /translation="MRRRPSLAATLVALVVVATTLIGSTPADHARGGDPLAEAQASQR
                     SLQESLAAQKRELDGLKALSSTLDARLAAAEAELASVSAEYERVAGLLVQVEQQIIEV
                     MARLDAIRARIKSLDEMLVALAIEVERQNRELEARETLLQDHYRDAYERSQTSLLEVL
                     LAADSFETVATQVGYLLTISEQDAAIADEIRELRATLEENRSVLREGRAELADARDVE
                     EAEAATLAARQAELAALKKRLGDLVVAAEQRRQEQAAALNAALTAQGDVERQIKATKE
                     AAAAANALVTRLLAESQVQVSSRGFRWPEDYFTVTQEWGPTSFVLEPPYTFNGTYYPH
                     FHGGIDLANGCGTRIRAAGTGVVVASGQPLWPWDTGYGVVIDHGGGVLTWYWHLKAQV
                     VVFSGQGVSIGDTIGYEGTTGMSTGCHLHFAVNDHGVWTNPRWYLP"
     gene            complement(3231..4130)
                     /locus_tag="VGO32_05355"
     CDS             complement(3231..4130)
                     /locus_tag="VGO32_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV7200213.1"
                     /translation="MTAIVGRVTERISAIADRYSIVGIGLEAFGKFNAHESMVRAAAI
                     AYYSILSLFPFALVAIVAASLFISPGPELDAFVARVANAFGLDPATVFGALDQFEDSR
                     LVFGAVGIGLLLLALLPWVSAVQRGIVTAFEEGRRSYIRTTLSSLLLLGLAGILILLS
                     GAWSSLVTLVFGFIDRLLPGMALVDITLGVAITLLPTLIVFGVMTALLKAVPSQDPSF
                     REVWLGASVTALGFFGLSQLFDLYIQLFVADSGNAAGPFGAILIGLLYIDFLAIAMLA
                     GTEVAAATTRRHRAPKTLESGPG"
     gene            4155..5501
                     /gene="ligD"
                     /locus_tag="VGO32_05360"
     CDS             4155..5501
                     /gene="ligD"
                     /locus_tag="VGO32_05360"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEV7200214.1"
                     /translation="MAPTKAVTLDVDGQTVSVSSPDKMFFAEAGVTKLDLVRYYLSVA
                     DGALRGVRDRPMAMKRFPNGAGSDFFFQKRAPANLPPFVRTVELSFPSGRTADEVVVS
                     NAAALAWVVNLGCIDLNPHPVRGNDLDHPDELRVDLDPGPGVEWSDVRAVALVCRELF
                     DELGYTSWPKTSGSRGIHVYVRIEPRWSYGEVRRAALAVAREVERRAPALATSKWWKE
                     ERHGVFLDYNQNAKDRTVASAYSVRPLPDARVSTPLTWGEVPDCDAADFTVKTVPDRF
                     ARIGDVGAGIDDHAYSLEPLLELVARHEADGLGDAPWPPNYAKQPGEPPRVQPSKARK
                     DVPSPTGRRRSSAPLVVVAQAATKPEVEEGLERWKKRHRKAAKLLAVDDVLYDAMRGR
                     STVWWRVRVNLRHVPEKDRPKAEPPDPDYDPRAGFEWSDRSDRPDHAPPKKKPASD"
     gene            complement(5510..6091)
                     /locus_tag="VGO32_05365"
     CDS             complement(5510..6091)
                     /locus_tag="VGO32_05365"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV7200215.1"
                     /translation="MLRPERVVLEGRVVRLEPLEERHRADLLAAAAEDPHIWDFMSSN
                     LADPTTWSAYLADAQGPDFVAWATVETATGQAVGGTRFGDIAPEHDRVEIGWTWIAPS
                     RHRTAVNSEAKLLQLTYAFETLGAGRVALKTDGRNERSQRAIERLGAVREGTLRRHMR
                     LPDGYVRDTVYYSILADEWPAVKARLTERLAQR"
     gene            complement(6096..>7752)
                     /locus_tag="VGO32_05370"
     CDS             complement(6096..>7752)
                     /locus_tag="VGO32_05370"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7200216.1"
                     /translation="ADRFWEGVLERDPITATIYGDDRYDERWPDLGPDGRAAERSALS
                     SALAEAEAIDPDALDVESVITRDLLILISRNYLEAQDRKLYQLAVNHMSGPQTWPSEI
                     AQYQRADTPESLDRMLARFAAFPTLIEQQIGTLDEGIADGRTSAAVPVQKAIEQIDRM
                     LTVPSDVAPAVVLANVADESGREAIRGSVVQDIYPALKRLRDYLAETYTPHAAPTPGL
                     GDTPGGHDAYGLAIRMQTTVDATPEEVHAFGMEDLERIEAEKDEIARRQGHADRHAFV
                     AALAEDPANRTDDPDHIIALAREQTDRAYATAPRFFGRLPAANCTVLAVEEYRERESA
                     PAFYMPPTIDGSRQGQYYLNTYQPEQRQLHKIAAITFHEATPGHHFQIAIEMDLQGLG
                     RFRTLGAKMAGSAYVEGWGLYTERLADEMGLYQTDAERLGMLEAQSFRAARLVVDSGL
                     HVMGWTRERAIEFMHERGSLPMVDAEIEVDRYTIWPGQALSYKMGQREIERARADVGE
                     RMGADFDLRAFHDEVLGHGSLPLETLRREIPGWVAAVTAGAGT"
BASE COUNT         1182 a         2651 c         2752 g         1167 t
ORIGIN      
        1 gtcgctgccg gtctactcgg gcgggctggg ggtgctggcc ggagacctgc tcaaggcggc
       61 gtccgatgca cgactgccga tgatcggcgt cgggccgttc taccaccgtg gctacttccg
      121 acagcgcatt gacgcggaag gccgacagga gcatctcgag cccgatgttg acccgcgcga
      181 cctccccctc cgacgcgcaa gcagcgcgga ggggagcccg ctcgagatcc gggtggagct
      241 gccgggtcgc gatgtccatc tggcgatctg ggttgcgcag gttggccgcg tgccgctgct
      301 cctgctcgat gccgacgtcg agggcaacga gcctgaggat cgccgggtca ccgacgagct
      361 gtacgtcgcg gcgcggcgaa cgcggctcac ccaggaagtg ctgctggggg tcggtgcggc
      421 gcgcgcgctg gatgcgctcg gcatccgtcc cgcggtctgg catctcaacg agggccactc
      481 ggcgttggtc gtgctcgagc gggcgcggct ggccggcgtg gccgatccca cgctcgatgg
      541 cgccgctcgc ctggcacgcg ccggccgcga catggtgatg accctccaca ccccggtgcc
      601 ggccgggaac gaatgctacg acccggcggt cgccgatgag ctgctcgcgc ccgcggcatc
      661 cgccatcgac ctcagttccg atcggctcca tgcgctcggc aaaggcccgg agggaacgaa
      721 cggcgtgttc gacctcaccg ccttcgggct gcgccaggcg gccgccatca acgccgtgag
      781 ccagctccac ggacggaccg cgaccgcgac ctggcggtcg gccatcggtc gagacgtcat
      841 atcggtgacc aatggcgtgc acctcgcgac ctggcaggcg cagccgatgc aggccgccct
      901 ccgggccgcc ggcgaggccg ccatcggtga ccgcgagctg tgggacgccc atgcagagca
      961 gaagcgccag atgatcgact tcctcggtgg acggctgacc cgtcaggcga tccgccacgg
     1021 cgtcgcgccc gaggccgttg gcgacctggc cggggccctg gatccacacg cgctgaccat
     1081 gggcttcgcg cggcgcttcg cgacctacaa acgggctgac ctcatcctgc acgacatgaa
     1141 gcgactggcc cacctgctcg ccgatccaga tcggccggtg cagctcatct tcgccggcaa
     1201 ggcgcatccg gcggacgagg ccgggcgcgc ggtcctgggc catgtggtgg acgcctcgcg
     1261 acgggtgggc ctcgccggcc gcatcttctt catcgaggac tatgacctgc gggtcgcgcg
     1321 gttcctggtg gccggagtgg atgtctggct gaacacgccg cgccgtccgc ttgaggcctc
     1381 cggcacctcg ggcatgaagg cggcgctcaa cggggtgccg tcagtgagca tcctcgacgg
     1441 gtggtgggac gaggcgtttg acggcacgaa cgggtgggcg gtcggacgcc catcgagcga
     1501 ggacggcggc accgacgagg aggacgccga gtcgctctat cgcatcctcg agaccgaggt
     1561 cgtgcccgcg ttctttggtc gaagcgacgt cgggatcccc gaggcatgga tcgggcgcat
     1621 gcggggggcg ctgacaaccg gcctggcgag gttcacggcc gatcgcgtgc tgcgggacta
     1681 cgtcgaggac ctgtaccggc cggtggccgg catggacggc gaggtcgccc ggtccgccta
     1741 gggcaggtac cagcgcgggt tggtccagac accgtggtcg ttgaccgcga agtggaggtg
     1801 gcagccggtg gacatgccgg tcgtgccttc gtagccgatc gtgtcgccga tcgagacgcc
     1861 ctgacccgag aacacgacga cctgggcctt caggtgccag taccaggtca gcacgccccc
     1921 accgtggtcg atcaccacgc catagccggt gtcccatggc cacagcggct gccctgacgc
     1981 gacgacgacg ccggtgcccg cggcccggat ccgcgttccg cagccgttcg cgaggtcaat
     2041 gccgccatgg aagtgcgggt agtacgtccc attgaaggta tagggcggct cgagcacgaa
     2101 gctcgtcggg ccccactcct gggtcacggt gaagtagtcc tcgggccatc ggaaaccgcg
     2161 ggagctgacc tggacctgcg actcggccag cagccgggtg accagggcgt tggcggcggc
     2221 ggccgcctcc ttggtggcct tgatctggcg ttcgacgtcg ccctgggcgg tgagcgcggc
     2281 attcagggcg gcggcctgct cctggcgcct ctgctcggcc gccacgacga ggtcgcccag
     2341 tcgcttcttc agcgcggcca gctcggcctg gcgggcggcc agggtcgcgg cctcggcctc
     2401 ttcgacatca cgggcatccg ccagctcggc gcgcccctcg cggaggacgg atcgattctc
     2461 ttcgagcgtg gcgcgaagct cgcggatctc gtcggcaatg gcggcgtcct gctcggagat
     2521 cgtgagcagg tacccgacct gggtggcgac cgtttcgaac gagtctgcgg caaggaggac
     2581 ctcgagcaat gaggtctggc tgcgctcgta ggcgtcgcgg tagtggtcct ggagcaatgt
     2641 ctcgcgcgct tcgagctcgc ggttctggcg ctcgacctcg atcgcgaggg cgaccagcat
     2701 ctcgtccagc gacttgatcc gggcccgaat cgcatcgaga cgagccatga cctcgatgat
     2761 ctgttgctcc acctggacaa ggagccccgc aacccgctcg tattcggcgc tcacgctggc
     2821 cagctcggcc tcggcggcgg ccaggcgggc atcgagggtg gacgagagtg ccttcaggcc
     2881 atcgagctcg cgcttctgcg ccgcgaggga ttcctggagg ctgcgctggc tggcctgggc
     2941 ctcggccaac gggtcgccac cgcgggcgtg gtcagccggc gtcgacccga tcagggtcgt
     3001 ggcgacgacc accagagcga cgagtgtggc ggccagagat ggccgacgcc tcatgagacg
     3061 gtattagtcg tagggatatt catcgtccca tccagccccc gcggaagaga tgcccgagga
     3121 tgctaccccg ggtcgtcaac ctcgggcgac gccgcgaatg tactacaccg taggacaccc
     3181 atcggtacca gagcacaccg gaccgcaccc gcgtacggtg cgcgtggagg tcagccgggc
     3241 cccgattcca gcgttttcgg cgcccgatgg cgccgggtcg tcgcggccgc cacctccgtg
     3301 ccggccagca tcgcaatcgc caggaagtcg atgtacagca ggccgatcag gatcgccccg
     3361 aacgggcctg ccgcattgcc gctgtcggcc acgaacagct ggatgtacag gtcgaacagc
     3421 tggctgagcc cgaagaatcc gagagccgtc accgacgccc ccagccacac ctcgcggaag
     3481 ctcgggtcct gggacgggac cgccttcaag agcgcggtca tgacgccgaa cacgatgagc
     3541 gtcggcagca gggtgatcgc cacccccagc gtgatgtcga ccagcgccat ccccggcaag
     3601 agccgatcga tgaacccgaa cacgagggtc accaggctcg accaggcgcc ggacaggagg
     3661 atcaggatgc ctgccaggcc gagcaggagc aggctgctca gggtggttcg gatgtaggac
     3721 cggcgccctt cctcgaatgc ggtcacgatc ccgcgctgga cggcggacac ccaaggcagg
     3781 agcgcgagga gcaacaggcc gatgccgacg gccccgaaga ccagcctcga gtcctcgaac
     3841 tggtcgagcg cgccgaacac ggtggccggg tccaggccga aggcgttcgc cacgcgagcc
     3901 acgaacgcgt cgagctccgg acccggggag atgaacagcg acgccgcgac gatggccacg
     3961 agcgcaaagg gaaacaggga caggatgctg tagtaggcga tcgcggcggc gcggaccatg
     4021 gactcgtggg cgttgaactt cccgaacgcc tcgaggccga tgccgacgat cgaataccga
     4081 tcggcgatgg cgctgatccg ttcggtgact cgacccacga tggcggtcat tgccactccc
     4141 gctaccctga cgccatggcg cccaccaagg ccgtcaccct cgatgtcgac ggacagaccg
     4201 tatcggtctc cagcccggac aagatgttct tcgccgaggc cggcgtaacc aagctggacc
     4261 tggtccgata ctacctgtcg gtcgccgacg gggcgctgcg cggcgttcgc gaccggccga
     4321 tggccatgaa gcgattcccg aacggggcag gcagcgattt cttcttccag aagcgggcgc
     4381 cggccaacct gccgccgttc gtccggacgg tggagctgtc gtttccttcc gggcggaccg
     4441 ccgacgaggt cgtcgtctcc aacgcggccg cgctggcctg ggtcgtgaac ctggggtgca
     4501 tcgacctcaa ccctcacccg gtccgcggca atgaccttga ccatcccgac gagctgcgcg
     4561 tggacctcga tcccggccct ggcgtcgaat ggtcggacgt gcgcgcggtg gcgctcgtct
     4621 gccgtgagct gttcgacgag ctggggtaca cctcatggcc caagacctcc ggctcccgcg
     4681 gcatccacgt ctacgtccgg atcgagcccc gctggtcgta cggcgaggtg cggcgcgcgg
     4741 cgctggccgt ggcccgcgag gtcgagcgcc gggcgcccgc cctggccacg agcaagtggt
     4801 ggaaggagga gcggcacggc gtgttcctcg actacaacca gaacgccaag gaccgaaccg
     4861 tggcgtcggc gtactcggtg cggcccctcc ccgacgcgcg ggtctcgacc ccgctcacgt
     4921 ggggcgaggt ccccgactgc gatgctgcgg acttcacggt caagaccgtc ccggaccgat
     4981 tcgcacggat cggcgacgtg ggagcgggga tcgacgacca cgcctattcg ctcgagcctc
     5041 tgctcgagct cgtcgcgcga cacgaggccg acgggctggg agacgctccg tggccgccga
     5101 actacgcgaa gcagccgggc gagccgcccc gcgtccagcc ttcgaaggcg cggaaggacg
     5161 ttccctcccc caccggccgg cgccgctcga gcgccccgct ggtggtcgtg gcccaggccg
     5221 cgaccaagcc cgaggtcgag gagggtctcg agcggtggaa gaagcggcac cgcaaggcgg
     5281 ccaagctgct tgccgtggat gacgtcctgt acgacgcgat gcgcggccgc tcgacggtct
     5341 ggtggcgggt ccgggtcaac cttcgtcatg tcccggagaa ggaccgtccc aaggcggagc
     5401 cacccgatcc cgattacgac ccacgggccg ggttcgagtg gtccgatcgg tccgatcggc
     5461 cggatcacgc gccgccgaag aagaagccag ccagcgactg agccgaaccc tagcgttgcg
     5521 cgaggcgctc ggtgagacgg gccttcaccg ccggccactc gtcggcgagg atcgagtagt
     5581 agaccgtgtc gcggacgtag ccgtcgggca ggcgcatgtg gcggcggagg gtcccctccc
     5641 gaacggcgcc cagccgctcg atcgcgcgct gtgaccgttc gttgcgccca tcggtcttga
     5701 gcgcaactcg ccctgcgccc agcgtctcga aggcgtaggt cagctggagg agcttggcct
     5761 cgctgttgac cgcggtccga tggcgggacg gcgcaatcca ggtccagccg atctccacgc
     5821 ggtcgtgctc cggggcgatg tcgccgaacc gcgtgccccc gaccgcctgg ccggttgccg
     5881 tctccacggt cgcccaggcc acgaagtccg gcccctgggc gtcggccagg tacgcggacc
     5941 aggtggtcgg atcggccagg ttggagctca tgaagtccca gatgtgggga tcctcggccg
     6001 ccgccgcgag caggtcagcg cgatgccgct cctcgagcgg ctcgagacgg acgacccgcc
     6061 cctcgaggac gacgcgctcc gggcggagca tcagctcagg tgccggcccc cgcggtcacc
     6121 gccgcgaccc agcccgggat ctcccgccgg agcgtctcga gcggcaacga tccgtgcccg
     6181 agcacctcgt cgtggaaggc gcggaggtcg aaatcggctc ccatccgctc gccgacatcg
     6241 gcccgtgccc gctcgatctc gcgctggccc atcttgtacg acagcgcctg gccgggccag
     6301 atcgtgtacc gatcgacctc gatctcggca tccaccatcg gcagcgagcc gcgctcgtgc
     6361 atgaactcga tggcgcgctc gcgagtccag cccatgacat gcagccccga gtccacgacc
     6421 agccgggcgg cgcggaagga ctgggcctcg agcattccga gccgttcggc atcggtctgg
     6481 tacaggccca tctcgtcggc cagccgctcg gtgtacaggc cccagccctc gacgtaggcc
     6541 gagccggcca tcttcgcccc cagcgtccgg aaccggccca atccctggag atccatctcg
     6601 atcgcgatct ggaagtggtg gccgggggtc gcctcgtgga aggtgatcgc cgcgatcttg
     6661 tgcagctgcc gctgttcggg ctggtaggtg ttcaggtagt actggccctg gcgcgagccg
     6721 tcgatggtcg gcggcatgta gaacgccggg gcggactcgc gttcgcggta ttcctcgacg
     6781 gccaggacgg tgcagttggc cgccggcaga cgaccgaaga accgcggcgc ggttgcgtac
     6841 gcccggtcgg tctgctcgcg ggcaagcgcg atgatgtggt ccgggtcatc ggtccggttg
     6901 gctggatcct cggccagggc ggcgacgaac gcatggcggt cggcgtgccc ctgccggcgg
     6961 gcgatctcgt ccttctcggc ctcgatccgc tcgaggtcct ccattccgaa ggcatggacc
     7021 tcttccgggg tggcgtcgac cgtcgtctgc atccgaattg caagcccata cgcgtcgtgg
     7081 ccgccgggcg tgtcaccaag gcccggcgtg ggagcggcgt gcggggtgta ggtctcggcc
     7141 aggtagtcgc gcagacgctt cagggcggga tagatgtcct gcaccaccga gccccgaata
     7201 gcctcgcggc cggactcgtc ggccacgttg gccaggacga cggccggggc gacgtcggat
     7261 ggaacagtca gcatccggtc gatctgctcg atcgccttct ggaccgggac cgcggccgag
     7321 gttcgaccgt cggcgatccc ctcgtccagg gtgccgatct gctgttcgat gagcgtcggg
     7381 aacgcggcaa agcgggccag catccggtcg aggctctcgg gcgtgtccgc gcgctggtac
     7441 tgggcgatct ccgacggcca ggtctggggc ccggacatgt ggttgacggc cagctggtac
     7501 agcttgcgat cctgcgcctc gaggtagttt cgggagatga ggatcaggag gtcgcgggtg
     7561 atgaccgatt cgacgtcgag cgcgtcaggg tcgatcgcct ccgcctcggc gagagcggag
     7621 gacagggcgc tccgctcggc ggctcggccg tcgggaccga ggtcgggcca gcgctcgtca
     7681 taccggtcgt cgccgtagat ggtggcggta atcggatcgc gctcgaggac gccctcccag
     7741 aaacgatcgg cg
//
LOCUS       DASYGU010000192         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_391943, whole genome shotgun sequence.
ACCESSION   DASYGU010000192 DASYGU010000000
VERSION     DASYGU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391943"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGO32_05375"
     CDS             complement(<1..285)
                     /locus_tag="VGO32_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200217.1"
                     /translation="MTAPETLPEAEGPDEMIRNGCHFLVALGLLGLAYLGGLAVNAVL
                     AIAIEAAAHSPGEETFLWQAVAAQTVGRFIMFALLTWFSWMVFRGPRRRAV"
     gene            321..923
                     /locus_tag="VGO32_05380"
     CDS             321..923
                     /locus_tag="VGO32_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200218.1"
                     /translation="MTDRTVRITRISTAAGYVAALAGVAALAGIGLSMSVGEPWGMLS
                     DTGLLILVGAVAPLMLAFYELGGVTPLRMAQLAQTLGWLSVGIFVVVELQVLAGSIDF
                     APDTATPDAVVAASLALGYIGLWIAGANLLAGPWLSAVRWLGVAAGLAAFLFGVGTAF
                     GGTDSGWRLLGGVGFVALGAVWAFLMARLLGERGNTTQEA"
     gene            923..1378
                     /locus_tag="VGO32_05385"
     CDS             923..1378
                     /locus_tag="VGO32_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7200219.1"
                     /translation="MASWDEFEHEAPEIAAAGRAALFRGGASEVFLTTVPGAGLPRMH
                     VVNVGIVSGRLLVFVQEKSAKAKDLTTDGRYALHAFQDPAVPDEFLLRGHALLVADGE
                     LRTRAVAGWPFKPADDYPLFELDIEHAILGARPSADDWPPKYTSWRAPA"
     gene            complement(1401..2090)
                     /locus_tag="VGO32_05390"
     CDS             complement(1401..2090)
                     /locus_tag="VGO32_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV7200220.1"
                     /translation="MALKEKLLANEEILFESKKHWISPVRDSIVPVLLIIGAYVLGIL
                     RGDGQGPLSFIGDIIGWIQIGLVVVGAAMIIYNIIAWRSATFAVSDLRVLREEGLISK
                     RSSATLLPSITDVKTKIPFLGSSLGYGDLEILTQGGEKGIDRFSSITQPEEFRDKVLN
                     QKVAGTAQAPAQAAAPTPAAPSAAPETQTSAAPGADDMTTLTRLAELRDSGVISAEEY
                     EAKKAEILSRI"
     gene            2151..2633
                     /locus_tag="VGO32_05395"
     CDS             2151..2633
                     /locus_tag="VGO32_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7200221.1"
                     /translation="MSFDHDDLIRGATFRPLSEVPQLQLSSASVRFTATGAETAGEFG
                     LFRYDMPAGPGGPSPHVHRTFSESFYVLDGVVDFYDGTGWVSAAAGDFLYVARNGAHA
                     FRNGGDGPASMLILFAPGEPRERYFKELAEIAASGRTLSPDEWTEVWARNDQFAVEES
                     "
     gene            2653..3048
                     /locus_tag="VGO32_05400"
     CDS             2653..3048
                     /locus_tag="VGO32_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7200222.1"
                     /translation="MATTIPDELLDLVTTNGLGHVSAIRRDGSMAHYVMWIDYDGQHL
                     LTSSMVGSLKAAHWRRNPAATISVVDRSNDWRYVIARGRVVEVRPDTGLAFIDKMSER
                     YTGGPYRFRERDREIFVIEIDHLIASGGR"
     gene            3117..>3763
                     /locus_tag="VGO32_05405"
     CDS             3117..>3763
                     /locus_tag="VGO32_05405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEV7200223.1"
                     /translation="MDDPLIPAILAAFVPALLWLAFFWSRDRYQREPTRAVLVTFAMG
                     AVLAVPLAIMVGIFIIPFVEGLGISDEGFIGLFILMFLLAGLPEEAIKGSLAAFRSTR
                     EVAVDEPADPMIYFTSLGLGFGALETVLYIVGTYAEVLSTGESAAFTEAFLVTAPLRA
                     LTVTMGHGLWTGIAGYCYAARRFNFGRRSGLVIGILLAAIFHAAYNTAVSTDLFLG"
BASE COUNT          571 a         1250 c         1277 g          665 t
ORIGIN      
        1 cacggcgcgc cgccgcgggc cacggaacac catccaggag aaccaggtca ggagcgcgaa
       61 catgatgaac cgacccactg tctgggctgc gacggcctgc cacaggaacg tttcctcccc
      121 agggctgtgg gccgcggcct cgatcgcgat ggcgaggacc gcgttgaccg ccaggccgcc
      181 gaggtaggcg agacccaaca gaccgagggc gaccaggaag tggcacccat tcctgatcat
      241 ctcgtcgggc ccctcggcct cgggcagggt ctcaggtgca gtcactcagg cgagcctagc
      301 agcggggata atcggcggga atgaccgatc gaaccgtgcg gatcacgcgg atcagcaccg
      361 cggccggata cgtggcggca ctggccgggg tcgcggcgct ggccggtatc ggtctgagca
      421 tgtcggtcgg cgaaccctgg ggaatgctga gcgacacggg cctgctcatc ctggtcggtg
      481 ccgtcgcgcc gctgatgctc gcgttctacg agctcggcgg ggtgacgccg ctccggatgg
      541 cgcagctggc ccagaccctg ggctggctgt cggtgggcat ctttgtggtc gtcgagctcc
      601 aggtgctcgc cggttcgatt gatttcgccc cggacactgc aacacccgat gcggtcgttg
      661 ccgccagcct ggcgcttggc tacatcggtc tgtggattgc cggcgcgaac ctgctcgccg
      721 gtccctggtt gtcggccgtc cggtggctcg gcgtcgctgc cgggctggct gcgttcctct
      781 tcggcgtcgg cacggcgttc ggaggtactg acagcggatg gaggttgctc ggcggggtgg
      841 gcttcgtggc gctgggagcg gtctgggcgt tcctgatggc ccgtttgctc ggcgagcgtg
      901 gcaacacgac gcaggaggcg tgatggcaag ctgggatgaa ttcgagcacg aagcccctga
      961 gatcgcggcg gccggacgcg cggcactctt ccggggagga gccagcgagg tcttcctgac
     1021 caccgtgccg ggtgcgggcc tgccgcggat gcacgtcgtg aacgtcggga tcgtgtcggg
     1081 ccggctcctc gtgttcgtgc aggagaagtc ggccaaggcc aaggacctga ccaccgacgg
     1141 ccgatacgcg ctccatgcct tccaggatcc ggccgtcccg gatgagttcc tgctccgtgg
     1201 ccacgcgctc ctggtagccg acggtgagct ccgtacacgg gcggttgcgg gctggccgtt
     1261 caagcccgcg gacgactacc cgctgttcga gctggacatc gagcacgcca tcctgggtgc
     1321 gcggccgtct gccgacgact ggccgccgaa gtacacctcc tggcgcgcgc ccgcgtagag
     1381 cgggggaccc ggtccgcggc ctagatccgg gagaggatct ccgccttctt ggcctcgtac
     1441 tcctcggcgc tgatgacgcc actgtcacgg agctcggcca gacgggtgag agtcgtcatg
     1501 tcatcggccc cgggggccgc actggtctgg gtctcggggg ccgcagatgg cgcagccggg
     1561 gtcggagccg cggcctgagc cggtgcctgt gccgtgcccg ccaccttctg gttcaggacc
     1621 ttgtcccgga actcctcagg ctgggtgatg gaggagaagc gatcgatgcc tttttcaccg
     1681 ccctgggtga ggatctcgag atcgccatat cccaggctcg aaccgaggaa cgggatcttc
     1741 gtcttcacgt cggtaatcga cgggagcaac gtcgccgatg accgcttgga gatcaggccc
     1801 tcctcccgga gaacgcggag atcgctgacc gcgaacgtgg ctgaccgcca cgcaatgatg
     1861 ttgtagatga tcatcgccgc gccaaccacg accaggccga tctggatcca gccaatgatg
     1921 tcgccgatga agctcaacgg accctggccg tcaccgcgga ggatgccgag gacgtaggcg
     1981 ccaatgatca ggagcacggg cacgatcgag tcgcggactg gcgagatcca gtgcttcttg
     2041 ctctcgaaca ggatttcctc gttggcaagg agcttctcct taagggccat gcccgcattg
     2101 taggaccggc tgtcacgccg accgcgcggg gcggagtagg cttcgggcca atgtccttcg
     2161 atcacgacga cctcattcgc ggagccacct tccggccgct cagcgaggtg ccgcaactgc
     2221 agctttcgag tgcctcggtc cgcttcaccg ccaccggcgc cgagactgcg ggagagttcg
     2281 ggctgttccg ctacgacatg cctgccggcc ccggaggacc gagcccccac gtccaccgca
     2341 cgttctccga gtcgttctac gtgctcgacg gggtggtcga tttctacgac gggaccggct
     2401 gggtctcggc cgctgcgggc gacttcctct acgtggcccg caacggcgct cacgccttcc
     2461 gcaacggcgg cgacggcccg gcatccatgc tgatcctgtt cgcgccgggc gagccccgcg
     2521 agcgctactt caaagagctg gccgagattg ccgcgtccgg tcggaccctc tcgcccgacg
     2581 agtggaccga ggtctgggcc cgcaacgacc agttcgcggt cgaggagtcg taggccgcta
     2641 ctatctcggc ctatggcgac cacgattccc gacgagctcc tggacctggt gacgacgaac
     2701 ggactcggtc atgtctcggc catccggcgg gacgggtcca tggcccatta cgtgatgtgg
     2761 atcgactacg acggccagca cctgctgacc agctcgatgg tcggttccct gaaggccgcc
     2821 cactggcgcc ggaatccagc ggccacaatc tcggtcgtcg atcggtcgaa tgactggcgg
     2881 tacgtgatcg ctcgaggccg agtggtggag gtgcgccccg atacgggcct cgcgttcatc
     2941 gacaagatgt ccgagcggta caccggcggg ccataccgat tccgggagcg cgaccgcgag
     3001 atctttgtca tcgaaatcga ccacctgatc gccagcggag gccgctgaac cgcacagcgg
     3061 agcatggcag cggaattcgg cgatggccgc tggtcggagc ctatgatccg cgaccagtgg
     3121 acgatcccct gatccccgcg atcctggcgg ccttcgttcc agccctgttg tggctcgcgt
     3181 tcttctggag ccgagaccga taccagcgag agcccacccg cgccgtcctg gtcacattcg
     3241 cgatgggcgc ggtgctggcg gtgccgctgg ccatcatggt cgggatcttc atcatcccct
     3301 tcgtcgaagg gctcggcata tccgacgaag gattcatcgg cctcttcatc ctgatgttcc
     3361 tgctcgccgg gctgcccgag gaggcgatca agggttcgct cgctgccttc cgctccacgc
     3421 gtgaggttgc ggtcgacgag ccggcggacc cgatgatcta cttcaccagc ctgggcctcg
     3481 gattcggggc gctcgagacg gtcctataca tcgtggggac ctacgccgag gtcctctcca
     3541 ccggcgagtc ggcggccttc acggaggcgt ttctggtcac cgcgccgctc cgggcgctga
     3601 ccgtgaccat gggccatggc ctctggaccg ggatcgccgg ctattgctac gccgctcgcc
     3661 ggttcaactt cggtcgacgc tcggggctcg tgatcggcat cctgctcgcg gccatcttcc
     3721 acgccgcgta caacaccgcg gtcagcacgg acctgttcct cgg
//
LOCUS       DASYGU010000193         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1780113, whole genome shotgun sequence.
ACCESSION   DASYGU010000193 DASYGU010000000
VERSION     DASYGU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780113"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VGO32_05410"
     CDS             <1..1363
                     /locus_tag="VGO32_05410"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV7200224.1"
                     /translation="NLTAIIDVNRLGQTGETMHGWDLDAYLRRAQAAGWHALAIDGHD
                     VVQIDRAYADAAATELRPTMIVARTEKGHGVAAVANQNGFHGKPLADPDAAIAELGGI
                     RSIRVQVNAPDRSVDIPAALEPGVVRLPAYELGTKEATRRAYGDALAAVGTARAEVVA
                     LDGEVSNSTYAEVFREAHPERYFEMYIAEQQLVATAIGMQVRGWRPFASTFSAFLSRA
                     YDFIRMAAVSRATMSLVGSHAGVSIGEDGPSQMALEDLAMMRAVHGSTVLYPSDPNQT
                     AALVAAMVDQPGISFLRTTREKTTVIYPPGEAFPIGGSRVVRSTGADHVTLVGAGITL
                     HEALAAADLLAGEGISARVVDLYSVKPIDEATLREAAAATGRLVTVEDHWPEGGIGDA
                     VLEVFADATERPRMTRLAVHSMPGSATPAQQLAAAGIDAAAIAAAARALVASASEPAP
                     VRG"
     gene            1378..2481
                     /gene="tal"
                     /locus_tag="VGO32_05415"
     CDS             1378..2481
                     /gene="tal"
                     /locus_tag="VGO32_05415"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV7200225.1"
                     /translation="MNTLQRLYAEFGQSPWVDSIDRDLIESGALATMIGDGIRGLTSN
                     PTIFAKAVAAGYYDELIRSMHENGTDGQAIYEAIAVGDIRDAADLLRPVYDAADGADG
                     LASIEVEPSLSEDGDGTLRRARELWAAVDRPNIFVKIPATGAGLPAIESATAEGINVN
                     ITLMFSVDVYQDVARAYIAGLRRLQAAGGDVSRIASVASFFVSRVDTKVDRLLDQAGT
                     EAAARARGTAAVANAQMAYQSFGEIFGGDEFADLRAAGARVQRCLWASTSTKDPSYPD
                     VKYVEALIGPDTVNTMPRETIAATLDHGRLARTLDHDFEAARAALDAVESLGISMRQV
                     TDELIREGVAAFATSFDELLATIEDKRRALATA"
     gene            2478..4145
                     /locus_tag="VGO32_05420"
     CDS             2478..4145
                     /locus_tag="VGO32_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200226.1"
                     /translation="MTDSLGEAVSRRLEQWAADRVAERIWARDGSLWAASGRPADEVA
                     EWLGWLDLPAMMGERIDEIRNLAAAVSRDGYRRAAVLGMGGSSLAPELCGRLFDGMTD
                     LRVCDSTHPDAVRGFRAWATAARSIQFVSSKSGTTTETLAFHAAMAEAVPALDFVAIT
                     DPGTPLTDLARAQEFRAIVEGEPSVGGRYSALSVFGLVPAAVRGVDVESLLGYARRMS
                     DACRARAADNPGLLLAATLGEAALVGRDKLTILTTPRLSTLGDWIEQLIAESLGKSGR
                     GIVPVVGEPVGPVEVYGADRCFVTITLDGEPDHGLAEVAAALREAGHPVGEFRLERPE
                     AVGGEFVRWEVAAAALGLVLEVNPFDQPDVASAKSATETLLEEFRRDGTFRLPMPLVS
                     EPGLSASADPARLGDTPPTVAAAVRSLLESVDDGDYFAVLAFLPPDRETWAALDRLRT
                     TVRDALGAATTVGIGPRYLHSAGQLHKGGPDRGVYLMLTADPDRDLPIPGRPESFGTL
                     IAAQAAGDFAALQARGRRILRVHLADATDGLARVGQLVAEAVGAPAA"
     gene            4174..>4838
                     /locus_tag="VGO32_05425"
     CDS             4174..>4838
                     /locus_tag="VGO32_05425"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV7200227.1"
                     /translation="MQVGIVGLGRIGAGMARRLGRGGHEVVAWNRTASVATDLAAEPE
                     NGGKVTAVGTVESLAEALSIPRHVLLSLPAGDATQELIDRLIPLLAEGDVIVDTGNAN
                     YRDSARRATELAAHGLTFMDMGVSGGVWGLEVGFCAMVGGDRAAFASLEPAIATLAPP
                     DGYLYCGPAGAGHYVKMVHNGIEYGLMEAYGEGFDILHASDYDLDLAAVARLWNHGSV
                     IRS"
BASE COUNT          710 a         1695 c         1699 g          734 t
ORIGIN      
        1 caacctgacc gcgatcatcg acgtcaatcg cctcggccag accggcgaga cgatgcacgg
       61 ctgggatctc gatgcctacc tccgtcgcgc gcaggcggcg ggatggcatg cgctcgcgat
      121 cgatggacat gacgtggtcc agatcgaccg cgcttacgcc gacgcagccg cgaccgagct
      181 gaggccgacg atgatcgtgg ctcggaccga gaagggccac ggcgtcgccg cggtggccaa
      241 ccagaacggg ttccacggca agcccctggc cgatccggat gccgccatcg ccgaactggg
      301 tggcattcgc tcgattcggg tccaggtcaa cgcacctgat cggtcggtcg acatccctgc
      361 cgcgctcgag cccggcgtcg tgcgcttgcc ggcctatgag ctcggcacga aggaagccac
      421 ccgtcgcgca tacggcgacg cgctggcagc cgtaggtacg gcgcgggcgg aagtggtcgc
      481 cctcgatggc gaggtcagca actcgaccta cgccgaggtc ttccgcgagg cgcaccccga
      541 gcgctatttc gagatgtaca tcgctgagca acagctggtg gcgaccgcga tcgggatgca
      601 ggtccgcggc tggcggccgt tcgcctcgac attttccgcg ttcctgagcc gggcgtacga
      661 cttcatccgg atggcggccg tcagtcgcgc gacgatgagc ctggtcgggt cgcacgccgg
      721 ggtatccatt ggcgaggatg gtccgtccca aatggcgctg gaggacctgg ccatgatgcg
      781 tgcggtccat ggcagcaccg tcctctaccc atccgatcca aaccagacgg cggcattggt
      841 ggcggccatg gtcgatcagc cgggtatctc gttcctgcgc acgacccggg agaagacaac
      901 ggtcatctat ccaccgggcg aggccttccc gatcggcggc tcacgggtcg tccgctccac
      961 cggcgccgac cacgtgaccc tggtcggggc ggggatcacc ctgcacgaag cgctggccgc
     1021 ggccgatctg ctcgccgggg agggcatctc cgcccgggtc gtggacctct acagcgtcaa
     1081 gccgatcgac gaagcgaccc tccgcgaggc cgcggcggcc accggtcgcc tggtcacggt
     1141 cgaggaccac tggcccgaag gggggatcgg cgatgcggtc ctcgaggtct tcgccgacgc
     1201 gaccgagcgc ccacgcatga cgcggctcgc cgtccattcc atgcccggat ccgcgacgcc
     1261 ggctcagcag ctggccgcgg ccgggatcga tgccgctgcg atcgcggcgg ccgctcgtgc
     1321 gctcgtcgcg tccgcgtccg agcccgctcc ggttcgcggc tgaggaggta tcggtccatg
     1381 aacaccctgc agcgcctgta tgccgaattc ggacagagcc catgggtcga ctcgatcgac
     1441 cgcgacctga tcgagtccgg cgccctggcc accatgatcg gggacgggat ccgggggctc
     1501 accagcaacc cgaccatctt tgccaaggcc gtggccgcgg gctactacga cgagctcatc
     1561 cggtccatgc acgagaacgg gaccgatggc caggccatct atgaggccat cgcggtcggc
     1621 gacattcgcg acgcagccga cctgctgcgg cccgtgtacg acgccgccga cggggccgat
     1681 ggcctggcct cgatcgaggt cgagccgtcg ctgtccgaag acggggatgg aacccttcgc
     1741 cgcgcacgcg agctgtgggc tgccgtcgat cggccgaaca tcttcgtgaa gattcccgcc
     1801 accggcgccg gtcttcccgc aatcgagagc gcgaccgcgg aggggatcaa cgtgaacatc
     1861 acgctcatgt tctcggtcga cgtctaccag gacgtggccc gcgcctacat cgccggcctc
     1921 cgccgcctcc aggcggccgg cggggacgtg tcacggatcg cgtcggtcgc gagcttcttc
     1981 gtcagccggg tcgataccaa ggtcgatcgc ctgctggacc aggcaggcac cgaggcagcc
     2041 gcccgggcgc ggggcacggc ggccgtggcc aacgcccaga tggcgtatca gtcctttggc
     2101 gaaatctttg gcggcgacga gttcgcggat ctccgcgccg ccggcgcgcg tgttcagcgc
     2161 tgtctctggg cgagcacctc cacgaaggac ccgtcctacc cggatgtgaa gtacgtggag
     2221 gcactcatcg gtccggacac ggtgaacacg atgccccgcg agacgatcgc cgccacactt
     2281 gaccacgggc ggttggcgcg aacgctggac cacgacttcg aggccgcgcg agccgcgctg
     2341 gatgcggtcg aatccctggg catctcgatg cgacaggtga ccgatgagct catccgcgag
     2401 ggcgtggcgg cgttcgccac ctcgttcgac gagctcctgg ccacgatcga ggacaagcgg
     2461 cgggccctcg ccacggcatg accgactctc tcggcgaagc cgtctcccgg cggctcgagc
     2521 agtgggccgc cgatcgcgtc gccgagcgca tctgggcacg cgacgggagc ctgtgggccg
     2581 cgagcggccg tccggccgac gaggtcgccg agtggctcgg atggctcgac cttccggcca
     2641 tgatgggtga gcggatcgac gagatccgaa acctggccgc ggccgtttcg cgtgacgggt
     2701 atcgccgcgc cgcggtgctc gggatgggcg gctcgagcct tgccccggag ctctgtggcc
     2761 gactgttcga cggcatgacc gacctgcggg tctgcgactc cacccacccg gacgcggtcc
     2821 gtggcttccg agcctgggcc acggccgccc gaagcatcca gttcgtgtcc tccaagtccg
     2881 ggaccacgac cgaaacactc gccttccacg ccgcgatggc cgaggcggtt ccggccctcg
     2941 acttcgtggc catcaccgac ccgggcacac cgctgaccga cctagcccgc gcccaggagt
     3001 tccgtgccat cgtggagggc gagccgtcgg tcggcggccg ctactcggcg ctgtcggtgt
     3061 tcgggctggt accggccgcc gtccgcggcg tggacgtcga gtcgctgctc ggatacgcac
     3121 gccggatgtc cgacgcgtgc cgcgcgcggg ccgcggacaa tcccggcctc ctcctggccg
     3181 ccacgctggg cgaggccgcc ctcgtcggcc gcgacaagct gacgatcctg accacgcctc
     3241 ggctgtcgac cctcggtgac tggatcgagc agctcatcgc cgagagcctt ggcaagagcg
     3301 gacgcgggat cgtgcccgtg gttggtgaac cggtcggccc ggtggaggtg tacggagccg
     3361 atcggtgctt tgtcacgatc accctcgacg gtgaacccga tcatggcctg gccgaggttg
     3421 ccgccgccct gcgtgaagcc ggccacccgg tcggggagtt ccggctcgag cggcccgagg
     3481 cggtgggcgg cgagttcgtg cgctgggagg tggccgcggc cgcgctcggt ctcgtgctcg
     3541 aggtcaaccc gttcgatcag cccgacgtcg catcggccaa gtcggccacc gagaccctgc
     3601 tcgaggagtt ccggcgcgac gggaccttcc gactgcccat gccgctcgtg tcggagcccg
     3661 gtctgtccgc ttcggcggat ccggcgcgac tgggcgatac gccgcccacg gtcgccgccg
     3721 cagtgcggtc cctgctcgag agtgtggacg acggcgacta cttcgccgtg cttgccttcc
     3781 tgccgccgga tcgcgagaca tgggccgcac ttgatcggct gcggacgacg gttcgcgacg
     3841 cgctgggcgc ggcgaccacg gtcgggatcg gtccgcgcta cctccattcg gcgggccagc
     3901 tccacaaagg gggccccgac cgcggggtgt acctcatgct caccgcggat cccgaccgcg
     3961 acctcccgat ccccgggcgg cccgaatcgt tcgggacgct gatcgcggca caggcggccg
     4021 gcgacttcgc cgcgctccag gcccggggcc ggcgaatcct gcgcgtgcat cttgccgacg
     4081 cgaccgacgg gctggctcgc gtcggccagc tcgtcgcaga agccgtgggc gcgcccgccg
     4141 cctaggtcat catccccatc gagaggagac gtcatgcagg tcggcatcgt gggactcgga
     4201 cggatcgggg cggggatggc gcgccggctg ggccgtggcg ggcacgaggt ggtggcatgg
     4261 aaccgaacgg cctcggtggc gaccgacctg gcggcggagc ccgagaacgg ggggaaggtg
     4321 accgcggtcg gaacggtgga gagcctggcg gaagcgcttt ccatcccgcg gcatgtgctc
     4381 ctcagcctgc cggccggtga cgccacccag gagctcattg atcgcctgat cccgctcttg
     4441 gccgagggcg acgtgatcgt ggacaccggc aacgcgaact accgcgacag cgcccggcgc
     4501 gccaccgagc tcgccgcgca cgggctgacc ttcatggaca tgggcgtctc gggaggcgtg
     4561 tggggactgg aggttggctt ctgcgcgatg gtcggtggcg atcgcgccgc cttcgccagc
     4621 ctcgagccgg cgattgccac ccttgcaccg cctgatggct acctgtactg cggaccggcc
     4681 ggtgccggcc actacgtgaa gatggtccac aacgggatcg agtacggcct gatggaggcc
     4741 tacggggagg gcttcgacat cctccacgca tccgactatg acctcgacct ggccgccgtc
     4801 gcccgtctgt ggaaccacgg cagcgtgatc cgatcctg
//
LOCUS       DASYGU010000194         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1162726, whole genome shotgun sequence.
ACCESSION   DASYGU010000194 DASYGU010000000
VERSION     DASYGU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162726"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGO32_05430"
     CDS             complement(<1..264)
                     /locus_tag="VGO32_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7200228.1"
                     /translation="MTWNRDRKRPRYFISIAAELAAVHPRTLRIYDEEGLVCPHRRNN
                     LRLYSEADIERVRIIRFLTRRQGVNLAGVKVILQLESIGKIRVY"
     gene            complement(261..683)
                     /locus_tag="VGO32_05435"
     CDS             complement(261..683)
                     /locus_tag="VGO32_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7200229.1"
                     /translation="MGHLTRFDPYRFLDDTYAGPMGWRYYLGETLTPLVDVHQTDDTV
                     AVTVSLPGVKPEDVNITLTGRTLVISGELKESEDIDGGQYVYRERRAGTFNRRIQLPV
                     RVIGEQADATFDNGLLRLSIPKAEESKPRQIEIKVPTA"
     gene            complement(783..2195)
                     /locus_tag="VGO32_05440"
     CDS             complement(783..2195)
                     /locus_tag="VGO32_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV7200230.1"
                     /translation="MWSHLPPEPIPLGDEPERKPAVAPGPARPSRLLPVVQCLAEGGL
                     LAVVAAAIQALFGEVPIIGPFEFAILAGIGMAWARRQRWRTPAWEAFGLPFLVIGAGT
                     LCWILAPEVRVALAGGNVQDALGSNPGGWIGAVAVYRGSVHRAREADEEIQDNVMRVG
                     LPVLAIAWLVGAVAARNVSPGVQDAFTAVAFIGSMVFVGAGVLALGLARLAAVRGDGP
                     GGGSWFGFALAVALGVTVLGVPAALLLGVPLEMLVVALVAPIRVIGALFVLLLSPIIL
                     LAALFVDMAQGILPAGFGAGTIRLPTIDVGIQQPDSPIAGILFYVLFGLLVLLELGAV
                     LLYLLWRWHDRRQLASLLSEVGEERQTVFERTPRPPRPPRPADPTPRDRHDPVGAYLL
                     ALEALAADDRWARHPAETPRRHLDRVGGTAGTPIGRELGRLAAAYQLIRYGGRRLGAR
                     EEARSAGRLDRLERAIRQTR"
     gene            complement(2173..3420)
                     /locus_tag="VGO32_05445"
     CDS             complement(2173..3420)
                     /locus_tag="VGO32_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7200231.1"
                     /translation="MTGWTALALAIVVAGAVAGVPGLFFVAVATFGYGATTRLWTRFG
                     LADLEYDRRLGTDRAVTGDTVDLEVAVWNRKALPLPWVAADDLVTDGLDVRERHALEL
                     DDVTVARRSLRNTWALGWYERVIRHYHLEARRRGVFEFGPVRLHVRDLVGRHARSEER
                     PLPGMLVVAPRTLPVRDVGAARAPIGERRARQSLVHDPSLFGGVRPFQAGDPLRRIHW
                     RATARVGRPVSRRWEPARSRQVIFVLDVQTLSGPAWEMSWDPDAFEGLCIAAASLARA
                     ALDSGAAVGLAAAGFSGTAQRIAYLPTRSGPAQLTRVTDLLARLGPISSGSLGGLLTW
                     LVRRVPPGASFVILTARPPQEIGPVLRRLAASGYGVDTLVLAGDPAASAAAVSSARRS
                     GIPARGMHLDPDWETADVVALTA"
     gene            complement(3417..>4033)
                     /locus_tag="VGO32_05450"
     CDS             complement(3417..>4033)
                     /locus_tag="VGO32_05450"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7200232.1"
                     /translation="ATPRTQSALLEAMQERQASVDGVTRPLPEPFIVLATQNPIELEG
                     TFALPEAQLDRFLLKIGMGYPSTASEQAIARRYQADAEPLDRVPTVGGPDTILSLRDA
                     VRQVRVADEVEAYLVSLVRATREHADVRLGASPRSSVALYRASQAWAAVEGRDFVLPD
                     DVRAVAHAVLDHRILLDLDRELRGATEEGVVDAVLDEVPVPLAE"
BASE COUNT          613 a         1443 c         1475 g          502 t
ORIGIN      
        1 gtagacgcgg atcttgccga tcgactcgag ctgcaggatg accttgacgc ccgccaggtt
       61 caccccctgg cgacgggtca ggaaacggat gatacgcacc cgttcaatat cggcctccga
      121 gtagagccgg aggttgttgc gccgatgcgg gcagacgagg ccttcctcgt catagatccg
      181 cagcgtgcgg ggatgcaccg ccgccagctc ggcggcgatg ctgatgaagt agcgcggccg
      241 cttgcggtcg cggttccagg tcacgcggtc gggaccttga tctcgatctg gcgcggcttg
      301 gactcctcgg ccttcgggat cgacaggcgc aggagaccgt tgtcaaacgt ggcatctgcc
      361 tgctcgccaa tgacgcgcac ggggagctgg atgcggcgat tgaacgtgcc ggcgcggcgc
      421 tcgcgataca cgtactggcc gccgtcgatg tcctcggact ccttcagctc gccgctgatg
      481 acgagcgtcc gaccggtgag cgtgatgttc acgtcctcgg gcttgacgcc ggggagggaa
      541 acggtcaccg cgaccgtgtc atccgtctgg tggacgtcca ccagcggggt gagggtttcg
      601 ccgaggtagt agcgccagcc catgggcccc gcgtacgtgt cgtcgaggaa tcggtagggg
      661 tcgaatcggg tcaggtggcc catggcaaac ctccgggaag ttgcgtggtt ctgtattcag
      721 gcggccggtc gacggccgct ccggcaccct aaaccctgac aataccgttg tcaactccta
      781 tatcaacgtg tctgacggat cgctcgttcc aggcggtcca ggcggcccgc cgatcgagcc
      841 tcctcacgcg cccccaggcg acgtccgccg taacgaatga gctggtaggc cgcggccagc
      901 cgaccgagct cgcggccgat gggcgttccg gccgtaccgc ccacgcggtc caggtggcgt
      961 cgcggggtct cggccggatg tcgcgcccag cggtcgtcgg cggcgagggc ctcgagggcc
     1021 agcagatacg cgccgaccgg atcgtggcga tcacgcggcg tcgggtcggc ggggcgtggg
     1081 gggcgcggcg ggcgaggcgt acgctcgaac accgtctgcc gttcctcgcc gacctccgac
     1141 aggagggacg cgagctgccg tcggtcgtgc cagcgccaca ggaggtacag gaggacggcg
     1201 cccagctcga gcaggacgag gagaccgaac agcacgtaga acaggatccc ggcaatcggg
     1261 ctgtcgggct gctgaatgcc cacgtcgatc gtcggcagcc tgatggtccc cgcgccgaag
     1321 cccgcgggga ggatgccctg ggccatgtcc acgaacagcg cggcgaggag gatgatcggg
     1381 gacaggagca ggacgaacag ggccccgatg acccggatcg gggccaccag cgccacgacc
     1441 agcatctcga gcggcacgcc gaggaggagg gcagcgggga cgccgagcac ggtcacgccc
     1501 agggccacgg cgagcgcgaa cccgaaccac gacccacccc ccgggccgtc gccgcggacg
     1561 gcggccagcc gcgccaggcc gagggccagc acacccgcgc cgacgaacac catggagccg
     1621 atgaacgcga ccgcggtgaa cgcgtcctgc acgccggggc tgacgttgcg ggcggccacc
     1681 gccccgacca gccaggcaat ggccaggacc gggaggccga cacgcatgac gttgtcctgg
     1741 atctcctcgt ccgcctcccg ggcccgatgg acggagccgc gatagaccgc gaccgccccg
     1801 atccagccgc cggggttgct ccccagggcg tcctgcacgt tgccgccggc gagcgcgacg
     1861 cggacttccg gcgcgaggat ccagcacagc gtcccggccc cgatgaccag gaacgggagt
     1921 ccgaacgcct cccacgccgg tgtccgccac cgctgacggc gcgcccacgc catcccgatc
     1981 ccggccagga tcgcgaactc gaagggaccg atgatcggca cctccccgaa gagggcctgg
     2041 atggcggccg ccacgacggc caggagccca ccctcggcga ggcactggac gacgggcagc
     2101 agccgggagg ggcgggcagg ccctggcgcg acggccggct tccgctccgg ctcgtcgccg
     2161 agcgggatcg gctcaggcgg taagtgcgac cacatccgcc gtctcccagt cggggtcgag
     2221 gtgcattccg cgggcgggga tcccggaccg gcgggcgctg ctgacggccg ccgcgctcgc
     2281 ggccgggtcg ccggccagca cgagcgtgtc caccccgtat ccgctcgcgg ccagccggcg
     2341 aagcacgggg ccgatctcct gcggcggccg cgcggtcagg atgacgaagc tggcacccgg
     2401 cggcacccga cggacgagcc aggtcagcag gccgcccagc gatccggagc tgatgggtcc
     2461 gagccgggcc aggaggtccg tcacgcgggt cagctgggct gggcccgacc gggttgggag
     2521 gtacgcgatc cgctgcgcgg ttcccgagaa gccggctgcg gcgagcccga ccgcggcacc
     2581 gctgtcgagg gcggctcggg ccagcgacgc ggcggcgatg cacagcccct cgaatgcgtc
     2641 ggggtcccag ctcatctccc aggccggccc ggagagggtc tggacgtcga ggacgaagat
     2701 cacctggcgg gaacgggcgg gctcccagcg gcggctgacc ggccggccga cgcgagccgt
     2761 cgcccgccag tggatgcggc gcagcgggtc gccggcctgg aacggccgaa ccccgccgaa
     2821 cagcgaggga tcgtggacca ggctctgccg cgcgcgccgc tcgccgatcg gagcgcgggc
     2881 cgcgcccacg tcgcggaccg gcagggtccg cggggccacg accagcatcc cgggcagcgg
     2941 ccgctcctcg ctccgcgcgt ggcggcccac gaggtcgcgg acgtggagac ggacggggcc
     3001 gaactcgaat accccgcgtc gccgagcctc gaggtggtag tgccggatca cccgctcgta
     3061 ccagcccagc gcccacgtgt tgcgcaggct ccgtcgggcg acggtcacgt cgtcgagctc
     3121 taatgcgtgg cgctcgcgca cgtcgagccc atcggtcacc aggtcgtctg cggcgaccca
     3181 cggcagcggc agcgccttcc ggttccagac cgccacctcg aggtccaccg tgtcgccggt
     3241 cacggcgcga tcggttccga gccgccggtc gtactccagg tcggccagcc cgaaccgggt
     3301 ccacagccgg gtcgtcgctc cgtagccaaa ggtggccacc gcgacgaaaa acagtccggg
     3361 cactccggcc acggcgcccg ccaccacgat ggcgagggcg agcgccgtcc agccggtcat
     3421 tcggccagcg ggacgggtac ctcgtcgagg accgcgtcca cgacgccctc ctcggtcgcg
     3481 ccccgcagct cgcggtcgag gtccagcagg atgcggtggt cgagcaccgc atgggcgact
     3541 gcgcgcacgt cgtcgggcag cacgaagtcg cggccctcga ccgcggccca cgcctgcgag
     3601 gcgcggtaca gcgccacgct gctccgcggc gacgccccga gccggacgtc cgcatgctcg
     3661 cgggtggccc tcaccaggct cacgaggtac gcctctacct cgtcggccac ccgcacttgg
     3721 cggaccgcat cacggaggct gaggatcgtg tccgggccac cgacggtggg cacccggtcg
     3781 agcggctcgg catcggcctg gtagcggcgg gcgatggcct gctccgacgc ggtgctcgga
     3841 tagcccatcc cgatcttgag caggaaccgg tcgagctgcg cctcgggcag ggcgaacgtg
     3901 ccctccagct cgatcgggtt ctgggtggcg agcacgatga acggctcagg cagcggccga
     3961 gtgacgccgt ccaccgacgc ctgccgctcc tgcatggcct cgagcagcgc ggactgcgtg
     4021 cggggcgtgg cgc
//
LOCUS       DASYGU010000195         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1574398, whole genome shotgun sequence.
ACCESSION   DASYGU010000195 DASYGU010000000
VERSION     DASYGU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574398"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VGO32_05455"
     CDS             complement(<1..679)
                     /locus_tag="VGO32_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7200233.1"
                     /translation="MTPCPNCGQPTPDDARFCANCGHALVTRVGAEERRHVTALFADL
                     VASTSLSDQLDPEVVRGVVSQFFERSIAEIRRFGGSAEQFRGDAIMALFGLQQAHEDD
                     PERAVRAAFAVRDGLAALAPEAAQRHGIALQLRIGIEAGEVVVGDPFGGSTMATGDVL
                     NLAARLEEHAAPGEIVVGPAVHAATERDIAYEPAGTWEIRGKAAPVSTWRAVAHTAVP
                     GGLRGLPG"
     gene            complement(735..1409)
                     /locus_tag="VGO32_05460"
     CDS             complement(735..1409)
                     /locus_tag="VGO32_05460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV7200234.1"
                     /translation="MSGLGDFGDSLRLAIAIGMTVFMVVLRFDQERIMRSDYFRSRSR
                     WMGPLSYYGLVLLFTAGIILILPRGRASLFLVGGDPADLVPAMIAFGVVGALNGAALA
                     LVRFRAILPLPLEMLPSRSVAAALNAVAEELQFRSVILGLMLVGGLNPSLAVLLQAIL
                     FGAAHRRVWRERDWYFVVGSVLLGYACGLVTISTQSVIPAMVGHFAITMGIYAFAGDR
                     LRMQRI"
     gene            complement(1409..>2948)
                     /gene="lepA"
                     /locus_tag="VGO32_05465"
     CDS             complement(1409..>2948)
                     /gene="lepA"
                     /locus_tag="VGO32_05465"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEV7200235.1"
                     /translation="SRSLQACEGAVLVIDASQGIEAQTLANAQLAMEQGLVIIPVINK
                     VDLASADPEMVKAELEETLAIPREEVILASAKDGTGVAAILEAIVSRIPAPPGDPAAA
                     LQALVFDSHYDPYKGVVCYVRLANGTLHDHDKIRFMATGISSEILELGWFRPQPVAAA
                     TLTVGEVGYVATGLKSIAEARVGDTLTTARHAAKEPLPGYRPALPLVFAGIYPMRGDE
                     YPLLRDALDRLHLNDASFVWEPESSVALGHGFRCGFLGLLHMEIVQERLEREFDLDLI
                     ASAPSVEYRVRRIGGKEVVVDNPAQMPDPGEIEEIAEPWVGLQVITPTEYVGPVMELA
                     TSRRGTFETMEYLDPRRVNLRFEMPLAELIVDFYDQLKGRSSGYASLDYTFIGYRSGD
                     LVKLDILVNGEPVDALSLIVHRSNASRIGRSLVEKLKALIPRQSFEVPVQAAIGSHII
                     SRETIRAYRKDVIAKCYGGDITRKRKLLEKQKEGKQRMKRVGSVEIPQEAFLAVLRMN
                     ETAS"
BASE COUNT          482 a          997 c         1033 g          436 t
ORIGIN      
        1 ggccgggcag cccacggagg ccgccgggca ccgcggtgtg cgccaccgca cgccaggtgg
       61 agaccggcgc ggccttgcca cggatctccc atgtcccggc tggttcgtag gcgatatcgc
      121 gctcggtcgc agcatggacc gcgggcccga ccacgatctc gcccggcgcg gcgtgctcct
      181 cgagccgagc ggcgaggttg aggacgtcgc cggtggccat cgtcgacccg ccgaacggat
      241 cgccgacgac gacctcgcca gcctcgattc cgatccgcag ctggagcgcg atcccgtgcc
      301 gctgggcggc ctcaggagcc agcgctgcca gcccgtcgcg gacggcgaac gcggcgcgga
      361 ccgcgcgctc gggatcgtcc tcgtgcgcct gctggagccc gaacagggcc atgatcgcgt
      421 cgccgcggaa ctgctccgcg ctgccgccga accggcggat ctccgcgatc gaacgctcga
      481 agaattggga aaccaccccg cgcacgacct cggggtcgag ctggtccgac agtgaggtcg
      541 acgccaccag atccgcgaac agggcggtga cgtggcgtcg ttcctcggcc cccaccctgg
      601 tcacgagcgc gtgcccgcag ttcgcgcaga accgggcgtc gtcgggcgtg ggttgtccgc
      661 agttggggca gggcgtcata ccgattcgag tgtaggcccg cgtgctggca gcggaaaccg
      721 tcccgccggt cgggtcagat gcgctgcatc ctcaaccgat caccggcgaa ggcatagatc
      781 cccatcgtga tcgcgaagtg accgaccatg gccggaatga ccgattgggt gctgatcgtg
      841 accaggccgc aggcatagcc gagcagcacc gagccgacca cgaagtacca gtcccgctcg
      901 cgccacacgc gccgatgggc ggccccgaac aggatggcct ggagcaggac ggcgagcgac
      961 gggttcagcc cgccgacgag catgaggccc aggatcaccg atcggaactg gagctcctcg
     1021 gccaccgcgt tgagcgccgc ggccaccgac cggctgggga gcatctcgag cgggaggggc
     1081 aggatggccc ggaaccggac gagggccagc gcggcgccgt tcagggcacc gaccaccccg
     1141 aacgcgatca tcgcggggac gaggtcggcg ggatccccgc cgaccaggaa cagcgacgcc
     1201 cggccgcggg gcaggatcag gatgatcccg gccgtgaaca ggaggacgag cccgtagtag
     1261 ctcagcggcc ccatccaccg cgatcgactt cggaagtagt cggatcgcat gatccgttcc
     1321 tggtcgaacc ggagcacgac catgaacacg gtcatcccga tcgcgattgc cagccggagg
     1381 gagtcgccga agtcgcccag gcccgacatc aggatgcggt ctcgttcatg cggagcacgg
     1441 ccaggaatgc ctcctgcgga atctcgaccg agccgacccg cttcatccgc tgcttgccct
     1501 ctttctgctt ctcgagcagc ttgcgcttgc gcgtgatgtc accgccgtag cacttggcaa
     1561 tgacgtcctt gcggtaggct cggatcgtct cgcggctgat gatatgggaa ccgatggccg
     1621 cctggaccgg cacctcgaac gactgccgcg ggatcagggc cttcaatttc tcgaccaggc
     1681 tgcggccgat gcgcgaggcg ttcgagcggt ggacgatgag gctcaacgca tccaccggct
     1741 cgccattgac caggatgtcc agcttgacca ggtcgccgga ccgatagccg atgaacgtgt
     1801 agtccaggct ggcgtagccg gatgatcggc ccttgagctg gtcgtagaaa tccacgatca
     1861 gctcggccag cggcatctcg aagcggaggt tcacccgtcg gggatcgagg tactccatcg
     1921 tctcgaacgt gccgcgtcgt gaggtggcca gctccatgac cgggccgacg tactcggtcg
     1981 gggtgatgac ctggaggcca acccacggct cggcgatctc ctcgatctcg ccggggtccg
     2041 gcatctgggc cgggttgtcg acgaccacct ccttgccccc gattcggcgg acgcggtact
     2101 cgaccgatgg ggccgaggcg atgaggtcaa ggtcgaactc gcgctccagc cgctcctgga
     2161 cgatctccat gtggagcagc cccaggaatc cgcaccggaa gccatgcccc agggccacgc
     2221 tcgactcggg ttcccacacg aacgaggcgt cgttgagatg gagcctgtcc agggcgtcgc
     2281 ggaggagcgg gtactcgtcg ccacgcatcg ggtagatgcc ggcgaacacg agcggcaggg
     2341 cggggcggta gccgggcagc ggctccttcg ccgcatggcg agcggtggtc agggtatcgc
     2401 cgacccttgc ctcggcgatc gacttcaggc cggtggccac gtaccccacc tcgccgacgg
     2461 tgagggtcgc cgcggcgacc ggctggggcc ggaaccagcc gagctcgagg atctcggagc
     2521 tgatcccggt ggccatgaat cggatcttgt cgtgatcgtg gagcgtgccg ttcgcgaggc
     2581 ggacgtagca gaccaccccc ttgtacgggt cgtagtggct gtcgaagacg agggcctgga
     2641 gcgccgccgc cgggtcgccg ggcggcgcgg ggatgcggga cacgatggcc tcgaggatgg
     2701 cggccacccc ggtgccgtcc ttggccgagg ccaggatcac ctcttcgcgg ggaatggcca
     2761 gcgtctcctc cagctcggcc ttgaccatct cgggatccgc gctggcaagg tcgaccttgt
     2821 tgatgaccgg gatgatgacc aggccctgct ccatggccag ctgggcattg gccagggtct
     2881 gggcctcgat cccctggctg gcatcgatga ccagcaccgc gccctcgcag gcctggaggg
     2941 accggctg
//
LOCUS       DASYGU010000196         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1522453, whole genome shotgun sequence.
ACCESSION   DASYGU010000196 DASYGU010000000
VERSION     DASYGU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522453"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VGO32_05470"
     CDS             complement(<1..1061)
                     /locus_tag="VGO32_05470"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV7200236.1"
                     /translation="MAKYVFVTGGVTSSLGKGITAASIGRILKARGIPVSILKLDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSQASNVTTGRVYQAVIAKE
                     RRGDYLGGTVQVIPHITNEIKERIARVAREGTGSVATSGLAQHGVVVVEVGGTVGDIE
                     SLPFLEAIRQMRKDVGRRNVLYVHVTWLPQIGATGEVKTKPTQHSVKELRGIGIQPDV
                     IVLRSDEPIEDDILDKVSLFTDVDVHAVIPLRTARSIYEVPLLLERAGLGKLITRELD
                     LPTTEPDLTDWQELVARIRAPRPEVEIAIVGKYTELPDAYISVSEALKHAGLHHRVDV
                     KVRWIGAEHFERVEPEEVAR"
     gene            1176..2009
                     /locus_tag="VGO32_05475"
     CDS             1176..2009
                     /locus_tag="VGO32_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV7200237.1"
                     /translation="MIQRAWAAIRLIHPAPALAVTALSAALAAILAREGVGPANTAAV
                     ILVTVSIAGSQVFTGATNDLADQARDATLRPEKPLAAGDLSASTALWIAAAGLAIQLV
                     TSGRLGTLPLALGAVASASAFVYNLWLSRTPLSVVPYVVSFGILPLWIGTGVGVPIER
                     VAPAVLLVAPFAAAAHLANTVRDFEADAAVGSYCLAQVLGRQAAHLLALVLALAVAIG
                     VAVSFVVAGQLDAASIVLGVLGLLAILRGWSDPDRLWQGMLAAAVMWTIAWGLASGPA
                     V"
BASE COUNT          283 a          710 c          736 g          376 t
ORIGIN      
        1 cgggcaacct cctcgggctc gacccgttcg aagtgctcgg cgccgatcca gcggaccttg
       61 acgtccaccc ggtggtggag gcccgcgtgc ttgagcgcct cgctgacgct gatgtaggca
      121 tctggcagct cggtgtactt gcccacgatc gcgatctcga cctccggtcg cggggcccgg
      181 atgcgggcca cgagctcctg ccaatcggtc aggtccggct cggtggtagg caggtccagc
      241 tcgcgggtga tgagcttgcc aagtcccgcc cgctcgagca gcaggggcac ctcgtagatg
      301 ctgcgggcgg tgcggagcgg gatgaccgcg tggacgtcca cgtcggtgaa gaggctgacc
      361 ttgtccaaga tgtcgtcctc gatcggctca tccgagcgga ggacgatcac gtcaggctgg
      421 ataccgatcc cgcgcagctc cttgaccgag tgctgggtgg gcttggtctt gacctcaccg
      481 gtggcgccga tctggggcag ccaggtgacg tgcacgtaca ggacgttgcg tctccccacg
      541 tccttgcgca tctggcggat ggcctccagg aagggcaagg actcgatgtc acccaccgtg
      601 ccgccgacct cgaccaccac caccccatgc tgggcgaggc cgcttgtcgc aacgctgccg
      661 gtcccctcgc gcgcgacgcg ggcgattcgt tccttgatct cgttggtgat gtgcgggatg
      721 acctgcaccg tcccgcccag gtagtcgcca cgccgctcct tggcgatgac ggcctggtac
      781 acgcggccgg tggtgacatt gctcgcctgg gacaggttct cgtcgatgaa ccgctcgtag
      841 tgccccaggt caagatcggt ctccgcgccg tcatcggtca cgaacacctc gccgtgctgg
      901 tacggcgaca tcgtgccggg atcgatgttg atgtaggggt cgagcttgag aatggagacc
      961 gggatgccgc gggccttcag gatgcgaccg atcgaggcgg cggtgatccc cttcccgagc
     1021 gaggaggtga cgccaccggt tacgaagaca tacttcgcca tggctgaatc acgggcctgt
     1081 tgcgagagct ggcagagcgt gcgggctgca cgcgggaagg gatcgacctt cacccacccg
     1141 ggtcacgatg ctaccccatt ccgtacgatg ccggagtgat tcaacgggcc tgggcggcca
     1201 tccgactcat ccacccggcg ccggccctgg ccgtgaccgc gctttcggct gccctggcgg
     1261 caatcctcgc ccgcgaaggc gttgggccgg cgaacaccgc ggccgtgatt ctggtgacgg
     1321 tctcgatcgc cggctcgcag gtgttcaccg gcgcgaccaa cgacctggct gaccaggcac
     1381 gggacgccac ccttcgcccc gagaagccgc tcgccgcagg cgatctgtcg gcctcgaccg
     1441 cactgtggat cgcggccgcc ggactggcca tccagctggt gacgagtggt cggctcggga
     1501 cgctcccgct cgccctcggg gcggtggcca gcgcctcggc ctttgtctat aacctctggc
     1561 tgtcgcggac cccgctgtcg gtcgtgccct acgtggtcag cttcgggatc ctgccgctgt
     1621 ggatcggaac cggggtcggg gtccccatcg aacgtgtcgc gccggccgtg ctgctggtcg
     1681 ctccgttcgc cgcggccgcc cacctggcga acaccgtccg cgacttcgag gccgacgccg
     1741 cggttggttc gtattgcctg gcccaggtcc tgggcaggca ggcggctcat ctccttgcgt
     1801 tggtcctcgc tctggcggtc gccatcggag tggcggtcag cttcgtcgtg gcggggcagc
     1861 tggacgccgc gagcatcgtg ctgggtgtgc tcggcctgct cgcgatcctg cgggggtggt
     1921 ccgacccgga ccggctgtgg cagggcatgc tcgcggcggc cgtcatgtgg acgatcgcct
     1981 gggggctggc cagcgggccg gccgtctagg gccggagcgc cggcctagat cgcgtcgtcg
     2041 atctcccagg gctcgtcgtc gacctcttcg gtcagcttga gccagacgaa gaggccggcg
     2101 aggac
//
LOCUS       DASYGU010000197         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_287917, whole genome shotgun sequence.
ACCESSION   DASYGU010000197 DASYGU010000000
VERSION     DASYGU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287917"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGO32_05480"
     CDS             <1..303
                     /locus_tag="VGO32_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200238.1"
                     /translation="PNDPCPDADRNRILVLALAATAVFGLMAGLAFVADYATHQRIMY
                     RGAWGRAARRGILAGLVLAAVAGLRLVDALNVFSALVVIVVAGAAEWLATRRLDAP"
     gene            300..1169
                     /locus_tag="VGO32_05485"
     CDS             300..1169
                     /locus_tag="VGO32_05485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7200239.1"
                     /translation="MTTDPAPGSSPDSGDPFGGQATDPDTLAEIYDLEHDAVTDDLGF
                     YRELTRRVRGAVLDLGCGSGRLFGAFLDGRASRVVGVDASSAMLRRAAARIARDTRLA
                     DALAEGQIELVHGDVRGVRRSERFGLIVAAGVLPHLNGPEDAARLLARAARLLTPRGL
                     LVLDDLGPASLPSSDLPLAVDWTVRSSDRTFTRRSQLVRREAPEGLRVTLSTVVDRQD
                     PDGTIARLPASFRLWYPSLDTVVRLVEAAGLALAVTHGSHDLEAFSSESERLIVLSRR
                     IRHRSGRRALETE"
     gene            1183..2331
                     /locus_tag="VGO32_05490"
     CDS             1183..2331
                     /locus_tag="VGO32_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7200240.1"
                     /translation="MAGERIRLLMIEDVPQVASHVRSLLIAQSQIQIVEVIGDGDRAL
                     AAVGDFRPDVVIIDWLLQGRVSGAKAASSIRQAHPDVGIIVLTVPQEPITEDPTRGID
                     AVLRMPLAGFDLTAVIRRVAEERVAQTTGGGAQMVSLFSPKGGVGRTTLAYNLAVALA
                     GEEPVCLVDGSLQFSDLRGLLRAPADARSILDLPTDKIKEGDVSEVMFRDPSGIDVLL
                     APPRVEMAEMITTRDIEKSLSLLRNLYGHVVIDTRAALTDEVLAFLDASDLVLQILTF
                     DAQAVRALAMANEAFAAIGYPPSKLAVVLNRADASGGMSREEVEEVLGQHIDFEIVSD
                     GRLVLAANNEGVPFVSASPDAQISKGVRSIAAALLAHRRSPAVSVAAR"
     gene            2337..3185
                     /gene="murI"
                     /locus_tag="VGO32_05495"
     CDS             2337..3185
                     /gene="murI"
                     /locus_tag="VGO32_05495"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV7200241.1"
                     /translation="MVSPDHRPIAVFDSGVGGLTVLSEIQRRLPGESTVYLGDNARTP
                     YGPRSADEVRAFTLESADWLLAQDPKVLVLACNTATAHALPQVREHVSVPVLGVVRPG
                     AVAAAAATRSRHVGVVATAGTVASGAYLAAIGDADPAVSVVQQACPDLVPLVEAGDLS
                     GPHVRIVVGGYLDQLRAQDPQQDTLLLGCTHYPLLRATFEQLLGPAVSVVDSAFTTAL
                     ALEDLLDALQARSGPRAGVPPQPNRVATTGDPETFRAVAVRLFGDGLPDVEAVELVSA
                     KAVRAD"
     gene            3195..4385
                     /locus_tag="VGO32_05500"
     CDS             3195..4385
                     /locus_tag="VGO32_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV7200242.1"
                     /translation="MSRTTARLTAGIVIGTVLTATVAVAGRRLEQRAGTHLLDWATIR
                     SIARRRVGDRRGALSPVERARIAAFYQAEAERVAPLVATEIGATLSTPFEPPAVIDRM
                     EWIDLNLSTFRQLIGRLEAELTGSRPTPRGTGPAIARIVNRSIGNHQLGWLLAFLAGK
                     VLGQYDISLLASAAPTRGRLYFVEANVRATADALGVDRATFRTFIAIHEVTHAYEFEA
                     HPWLRTHFASLVEETIHQLAADAGGMWSRLTQAANGSGHWMERLMTPAQRAAFERTQA
                     LMSLLEGASNHVMHQVGARILPEFDELHERFEGRHRRRGPLEQLILRLTGLDLKMEQY
                     AEGERFVAAVVAGSGTEGLARVWRGPEWLPTMAEIREPDAWLARADALDGTGGRTAGG
                     EHRP"
BASE COUNT          603 a         1691 c         1595 g          698 t
ORIGIN      
        1 ccaaatgacc cctgccccga cgccgaccgc aaccggatcc tggtccttgc cctggccgcg
       61 accgcggtct tcgggttgat ggccgggctc gctttcgtgg cggactacgc cacgcaccag
      121 cggatcatgt atcgaggcgc gtggggacgg gcggcgcggc ggggaatcct ggccggcctc
      181 gtcctggccg cggtcgctgg acttcggctc gtggacgcgc tcaacgtctt ctcggcgctg
      241 gtcgtgatcg tggtcgccgg cgcggccgag tggctggcga cgcgccggct ggacgccccg
      301 tgaccaccga tcccgctccg ggctcgagcc cggactcggg agatccgttc ggggggcagg
      361 cgaccgaccc ggacacgctg gccgagatct acgaccttga gcacgacgcc gtcaccgacg
      421 acctcggctt ctaccgcgag ctcacccggc gggtgcgggg cgcggtgctc gacctgggct
      481 gtggatccgg ccgcctgttt ggcgcgttcc tcgatggtcg agcgagccga gtggtcgggg
      541 tcgatgcctc gtccgcgatg ctgcgccggg cggcggcccg gatcgcgcgc gacacgcgcc
      601 tggccgatgc cctggccgaa ggccagatcg agctggtcca cggggacgtc cgtggcgtgc
      661 gccgatccga gcggtttggc ctgatcgtgg ccgccggggt ccttcctcac ctgaacggcc
      721 cggaggacgc ggcccgcctc ctcgcccgcg ccgcgcgcct gctcacccct cgcggcctgc
      781 tggtcctcga cgacctcggt cccgcgtcgc ttccgtcgtc cgatcttccg ctggccgtcg
      841 actggaccgt ccgttcgagc gaccgaacgt tcacccggcg cagccagctc gtgcgtcgcg
      901 aggcacccga gggactgcgc gtgaccttgt ccacggtcgt ggatcggcag gaccccgatg
      961 gtacgatagc gcggcttccg gccagtttcc gcctctggta cccctctctt gacaccgtcg
     1021 tgcggctggt tgaggccgca ggactcgctc ttgccgtgac gcacggatcg catgatctgg
     1081 aggcgttttc gagcgagagc gagcgcctga tcgtcctcag ccggcggatc cggcaccggt
     1141 ccgggcgacg ggcattggaa accgaatgag gcgaggcagc gagtggcggg agagcggatc
     1201 cggctcctga tgatcgagga cgtgccgcag gtggcgtcgc acgttcggtc gctgctcatt
     1261 gcccagagcc agatccagat cgtcgaggtc atcggtgacg gtgaccgggc gctggctgcg
     1321 gtcggcgact tccgccccga cgtggtcatc atcgactggc tgctccaggg ccgggtgagc
     1381 ggcgccaagg cggcctccag cattcgccag gcccatcccg acgtggggat catcgtgctc
     1441 accgtgccgc aggagccgat caccgaggat ccgacccgcg gcatcgacgc cgtcctccgc
     1501 atgccactcg ccggtttcga cctgacggcg gtcatccgcc gtgtcgccga ggagcgggtc
     1561 gcgcagacga ccggtggtgg cgcccagatg gtcagcctgt tctcgccgaa gggcggcgtc
     1621 ggccgcacga cgctcgccta caacctggcc gtcgcgctgg cgggcgagga gccggtctgc
     1681 cttgtggacg gctcgctcca gttctccgac ctccgcggcc tcctccgcgc tccggccgac
     1741 gcccggtcga tcctcgacct gcccaccgac aagatcaagg agggtgacgt gtcggaggtg
     1801 atgttccgcg acccgtccgg gatcgacgtc ctgcttgccc cgcctcgggt cgagatggcc
     1861 gagatgatca ccacccgcga catcgagaag tcgttgtcgc tgctgcggaa cctgtacggc
     1921 cacgtcgtga tcgacacgcg agcggccctg accgacgagg tgctcgcgtt cctcgacgca
     1981 tccgacctcg tcctccagat cctgaccttc gacgcccagg cagtgcgtgc gctggccatg
     2041 gccaacgagg ccttcgccgc catcgggtac ccgccgtcca agctggcggt ggtcctcaac
     2101 cgggccgacg cctcgggcgg aatgagtcgg gaggaagtcg aggaggtgct cggccagcac
     2161 atcgacttcg agatcgtgtc cgacggccgg ctggtgctgg ccgcgaacaa cgagggcgtg
     2221 ccgttcgtgt cggccagccc cgacgcccag atctccaagg gcgtgcggag catcgcggcc
     2281 gccctcctcg cccaccgccg ctcgccggcc gtgtccgtgg ccgcacgctg acggccgtgg
     2341 tctcgcccga ccaccgtccg atcgccgtct tcgactccgg agtcggcggc ctcacggtcc
     2401 tttccgagat tcagcgccgg ctgcccggcg agtcgaccgt ctacctcggc gacaacgccc
     2461 ggaccccgta tgggccgcgc tccgcggacg aggtgcgcgc cttcaccctc gaaagcgcgg
     2521 actggctcct ggcccaggat cccaaggtcc tcgtcttggc ctgcaacacg gccaccgccc
     2581 atgcactgcc ccaggtgcgt gagcacgtct ccgtcccggt cctcggcgtc gttcgcccgg
     2641 gggccgtcgc ggcggccgcg gcgacccgca gccggcacgt cggggtggtg gccacggccg
     2701 gcacggtggc atcgggtgcc tacctggcgg ccatcggcga cgccgacccg gcggtgagcg
     2761 tcgtccaaca ggcctgcccg gacctcgtcc cgctggtgga ggcgggggac ctgtccggcc
     2821 cgcacgtgcg gatcgtggtc gggggttacc tcgatcagct gcgggcccag gatccgcagc
     2881 aggacaccct gctcctcggc tgcacccact acccgctcct gcgcgccact ttcgaacagc
     2941 tgctcgggcc cgcggtcagc gtggtggact cggccttcac caccgcgctt gccctcgagg
     3001 acctgctcga cgcgctccag gcgcggagcg ggccacgagc cggcgttcca ccgcagccca
     3061 accgggttgc caccaccggc gatcccgaga cgttccgggc ggtggcggtc cgcctcttcg
     3121 gcgacggcct gccggacgtc gaagcggtgg agctggtttc cgcgaaggcc gtccgcgccg
     3181 actagactgc gcccgtgagc cgcaccacgg cccggctgac cgccgggatc gtcattggca
     3241 ctgtcctcac cgccaccgtc gcggtcgccg gtcgccgcct cgagcagcgg gccgggaccc
     3301 acctgctcga ctgggccacg atccggtcca ttgcccgccg tcgggttggc gatcggcgcg
     3361 gagcgctgtc gccggtggaa cgcgcccgga tcgcagcgtt ctaccaggcc gaggccgagc
     3421 gcgttgcacc cctggtggcc accgagatcg gggcgaccct cagcaccccg ttcgagccgc
     3481 cggcggtgat cgaccggatg gagtggatcg acctcaacct gagcacgttc cgccagctca
     3541 tcggccggct ggaggccgag ctcaccgggt cacgtcccac gccgcgcggc accggcccgg
     3601 ccatcgcccg aatcgtgaac cggagcatcg gcaaccacca gctcgggtgg ctgctcgcgt
     3661 tcctggccgg caaggtgctc ggccagtacg acatcagcct cctcgcgtcg gccgcgccga
     3721 cccgcggccg cctgtacttc gtggaggcca atgttcgggc aaccgcggac gccctcggcg
     3781 tggaccgggc cacattccgc acgttcatcg ccatccacga ggtcacccac gcgtacgagt
     3841 tcgaggcgca cccctggctg cgcacccatt tcgcctcact cgtggaggag acgatccacc
     3901 agctggctgc tgacgcgggc gggatgtggt cccgcctgac ccaggccgcg aacggttcgg
     3961 ggcactggat ggagcgactc atgaccccgg cccagcgcgc ggcgttcgag cggacccagg
     4021 ccctgatgtc gctgctcgaa ggggcctcga accacgtcat gcaccaggtg ggcgcgcgga
     4081 tcctgcccga gttcgacgag ctgcacgagc ggttcgaggg ccgccaccgg cgccggggac
     4141 ccctcgagca gctcatcctg cgcctgaccg gcctcgacct gaagatggag caatacgccg
     4201 agggagagcg cttcgtggcc gccgtggtgg cgggcagcgg cacggaggga ctggcccgcg
     4261 tctggcgtgg ccctgaatgg ctgccgacca tggccgagat ccgcgagccc gacgcgtggc
     4321 tcgcacgggc agacgccctc gacggcacgg gtggccggac cgcgggcggg gagcaccgcc
     4381 cctgaccacc ggccctgagg tcaggatcct caccaccccg atcttcggtg cgcccctgac
     4441 cccggacatg gtggcgcgga tcgaggcggt ccccggcgca cgggtgatcc ccatctcccg
     4501 tgacgggttg gcccacggtg acgacgaggc ggcgattgcg gaggcccagg ttctgctccg
     4561 cggaccgatc gcgtcgtcgg ttctcga
//
LOCUS       DASYGU010000198         6764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1317490, whole genome shotgun sequence.
ACCESSION   DASYGU010000198 DASYGU010000000
VERSION     DASYGU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317490"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VGO32_05505"
     CDS             complement(<1..1132)
                     /locus_tag="VGO32_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200243.1"
                     /translation="MRPSRIAIVAHASLPADPRIRRHAEALRDAGHDVDLIGLRDLGE
                     AEEEIWNGIRIVRLPVRRTFSGFAGHLAEYLAFAGLASVRLAVEHRHRRYRVVQVATL
                     PDFLAFAAMPVRLTGVPVLLDLHEDMPEFFRDRFASGGLRPLFPIISGTARVSAAVAD
                     AIITVHEPLRQLAIRRGVPAHRISVVMNSADERLFDPAGHPRRAWRADGALRLVHASS
                     LLPIYGLDTAVEAMARLDSALNAHLDVYGDGPFRTDVEDAITRYGADDRVTLHGRVPL
                     DALPDALAAADLALVPTRPEPYMAYSLSTKLLEAVAMAVPVIATDLATVRRHFDRAAI
                     RYVPGADAPALAAAIESLGSDPAAAAAQAAAASEQARPYAWTS"
     gene            complement(1129..2250)
                     /gene="mazG"
                     /locus_tag="VGO32_05510"
     CDS             complement(1129..2250)
                     /gene="mazG"
                     /locus_tag="VGO32_05510"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEV7200244.1"
                     /translation="MTGAARPVDELLTAAGLDPAAGVQIVSVDHLASTPFDPGLPLLV
                     LAATRSDVPVEDPLPGRHAHRGPRAVLRALYPAGHLLRALPGGESRPLEAIDDAALRA
                     GDWAVPALAATDNLASPHGMAAISARLRAPDGCPWDRRQDHRTLRPYLLEEAYETLDA
                     IERGTPADLAEELGDLYLQVVLHAQLAAEDGEFDLTDVYRTLGAKIIRRHPHVFGDVA
                     VTGVDQVVTNWETIKSEEREDAGRPASSFADVARTLPALPASREIQERASALGWDWPT
                     VDGVWEKVAEELDELRAAAGESSPADAQLHELGDVLFALANLARWLHVDPEEALRVAN
                     RRWVDRYGAVESLVADRGLVLADLDADAKDALWNEVKAR"
     gene            complement(2261..4024)
                     /locus_tag="VGO32_05515"
     CDS             complement(2261..4024)
                     /locus_tag="VGO32_05515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7200245.1"
                     /translation="MTYRNRPAPRRRHRARWQDELRTQRLLVGGFAAAIAVALGLFGI
                     SAWNAYYDTHLQQVMYVEGTAVIREELDLRKAIQAAELQATGVDIQDQLGEDQTTGGA
                     RGAVLQQQLSVITDQFSNLTATSTGSISDGLFQATRAEEFGISVTDEEIDAEVATRQT
                     LPARIQVSIIEVDALPEDAASGAEATDEDFARAEEEANDIRARLDDGEEFGAIATAES
                     DDPGSATFEGLVGWVSEDDPQYGYLFPLADGVEAGELSGPTRTDDGYVILRVEDRTEE
                     GPFTGLLDLLSSARITDADYRAYIGDELLKRDFRTYFTDDVAVSPAEQREVAQILILN
                     DQGVPVPKQRIRHLLAQPVPGGEVADQEAATEEQWAAAEARAQGWFDEVQDPDADWFE
                     IAKESDDPGSRSNGGDLGWYDPTTGQFVPEFEAAVARLAVGEISEPVRTDFGYHVIQV
                     TDQRTTALDFAASLIEDLEADPDSFGAEARANSEDSATRSDEGYLGWVARYEGSVARE
                     QAIFGMTDIGEISADPVVDGNQIWIFKLLGSNPERLVEESRLSTIQSTGYSRWYETLK
                     ADGQIWIDTQLQLEAAPPPTA"
     gene            complement(4100..4858)
                     /locus_tag="VGO32_05520"
     CDS             complement(4100..4858)
                     /locus_tag="VGO32_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEV7200246.1"
                     /translation="MGLAAGRGVWIVMPTFNERENIVGVVEAVLAALPEAQVLVVDDQ
                     SPDGTGELADTIAAANARVEVLHRPGKEGLGVAYRDGFKRVLDKPGTMAVIQMDADFS
                     HDPADLPRLVAPLMRDADLVLGTRWMPGGGTSGWPIHRRIISRGGTLFARIVLLLPYR
                     DLTGGFKAWRRELLEAIRLREAAGQGYGFQVETTWWAHRRGAAIREVPILFRERTAGR
                     SKMSGSIVTEAVVLVLRLRWQAVIRAVRRPRGDR"
     gene            complement(4888..6162)
                     /locus_tag="VGO32_05525"
     CDS             complement(4888..6162)
                     /locus_tag="VGO32_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV7200247.1"
                     /translation="MKIAVVGLGHIGLPLAVQYASRGHEVVGCDLDARVVEAINRGES
                     PHDDEAEVANQVAGLVRAGRLRATTDDSTGVREAEVVVVIVPVVVDESRQVDFSQIDA
                     ATRDVAHGLQAGTMVIYETTLPIGTTRERLGPMLEAGSGLRMAEDFDLAFSPERVLVG
                     RVLLDLRRYPKIVGGTSDQATRRAVAFYGDVLEPGTEILAMASAEAAEMTKLAETTYR
                     DVNIALANEFAQVADRRGIDVTEIIAAANSQPYSHIHQPGVGVGGHCIPVYPHLLFRD
                     EPQMRIPPLSRAINDEMAAYAVGQLGEVLGPLDGVPVLVLGIGYRGDVREDFFSSALL
                     LREALVAAGATVHGHDPYFDAEHLLRRGFVPYDLSAPVPVRAVILQAAHTAYRDLDPT
                     AFPGLEVLLDGRNVLDRARVEAAGVRYLGVGR"
     gene            complement(6159..>6764)
                     /locus_tag="VGO32_05530"
     CDS             complement(6159..>6764)
                     /locus_tag="VGO32_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7200248.1"
                     /translation="IRELGRRLHAGELGRVFQIRAARLGPFPARVRDVGVVVDLAPHD
                     LDVMRYLLRAEPIRMYAETEQRIHTDHEDLFTGLIKFDTGAVGLLEINWLTPTKVRKL
                     TVTGERGMYEADYLTQDLVFYANPDHELVSEGEMTRRAVQRREPLAVELSEFAAAIRS
                     GGPPPVEPRDALVAMLLARAMVDAAARGVVIGADELTPILA"
BASE COUNT          973 a         2362 c         2441 g          988 t
ORIGIN      
        1 gggatgtcca cgcgtatggc ctggcctgct cggaagcggc ggcggcctgt gcggcggcgg
       61 cggccgggtc cgagccgagc gactcgatcg cggccgcgag tgccggcgca tcggcgcccg
      121 ggacatatcg gatcgccgcg cggtcgaaat ggcgacggac ggtggccagg tcggtggcga
      181 tgaccgggac cgccatggcc acggcctcga gcagcttggt cgagagggag taggccatgt
      241 atggctcggg ccgggtgggc accagcgcga ggtcggcggc ggcgagcgca tcgggcaggg
      301 cgtcgagcgg gacccgcccg tggagcgtga cccggtcatc cgcgccgtac cgcgtgatcg
      361 cgtcctcgac atcggtccgg aacggcccgt cgccgtagac gtcgagatgc gcattcaggg
      421 ccgaatcgag gcgggccatg gcctcgaccg cggtgtcgag cccatagatc gggagcaggc
      481 tcgacgcgtg gaccagcctg agcgcgccat ccgcccgcca cgcccgccgg gggtgacccg
      541 cggggtcgaa cagccgctcg tcagcgctgt tcatgaccac cgagatccga tgggcgggaa
      601 cgccgcgtcg gatcgcgagc tggcgcagcg gttcgtggac ggtgatgatc gcgtcggcca
      661 ccgccgcgct gacccgcgcc gtgcccgaga tgatcgggaa caggggacgg agtccgccgg
      721 acgcgaaccg gtcccggaag aactcgggca tgtcctcgtg gaggtcgagc aggaccggga
      781 ccccggtcag ccggacgggc atcgcggcga aggccaggaa atcaggaagg gtggccacct
      841 ggaccacgcg gtagcgccga tgtcggtgct cgaccgcgag ccgcaccgag gcgaggcccg
      901 cgaacgcgag gtactcggcc aggtgcccgg cgaagccgct gaacgtcctc cgcaccggca
      961 ggcggacgat ccggatgcca ttccagatct cctcctcggc ctcgcccagg tcgcgcaggc
     1021 cgatgaggtc gacgtcgtgg ccggcgtccc gcagcgcctc cgcatggcgc cggatgcgcg
     1081 gatcagcggg cagcgaggcg tgggccacga tcgcgatccg gctcggcctc atcgggcctt
     1141 gacctcgttc cacagcgcgt cctttgcgtc ggcgtccagg tcggcgagga cgagcccgcg
     1201 gtcggcgacc aaggactcaa ccgcgccata gcgatccacc cagcgccggt tcgcgacgcg
     1261 gagggcctcc tccgggtcca cgtgcagcca gcgggccagg ttggcgaggg cgaagaggac
     1321 gtcgccgagc tcgtggagct gggcatcggc cgggctggat tcgccggcgg ccgcgcgcag
     1381 ctcgtccagc tcttcagcga ccttctccca tacgccgtcg acggtcggcc agtcccatcc
     1441 cagcgccgac gcccgttcct ggatctcacg gctggccggc agggcgggca gggtacgggc
     1501 cacgtcggcg aacgaggacg ccggtcgacc ggcgtcctcg cgctcctcgg acttgatcgt
     1561 ctcccagttg gtgaccacct ggtccacccc ggtcacggcc acgtccccga acacgtgcgg
     1621 gtggcgccga atgatcttgg cgccaagcgt gcgatagacg tcggtgaggt cgaactcgcc
     1681 gtcctcggcg gccagctggg catggagcac gacctggagg tacaggtcgc caagctcctc
     1741 ggcgaggtcc gccggcgttc cgcgctcgat cgcgtcgagg gtctcgtacg cctcctcgag
     1801 caggtacggg cgcaatgtcc ggtgatcctg gcgacggtcc cacgggcagc catccggggc
     1861 acgcaggcgg gcgctgatcg cggccatgcc gtgtggcgag gcgaggttgt cggtggctgc
     1921 cagggcgggc acggcccagt cacctgcccg cagcgccgcg tcgtcgatcg cctccagcgg
     1981 ccgcgattcg ccccctggca gcgcccgcag gaggtggccg gcgggataca gcgcccggag
     2041 caccgcgcgc ggcccacgat gggcgtggcg gccagggagc gggtcctcca ccggaacgtc
     2101 cgatcgggtg gcggccagga ccaggagcgg gaggcccgga tcgaacggcg tcgaggcgag
     2161 atgatcgacg gacacgatct gcacaccggc cgccggatcg aggccggccg cggtgagaag
     2221 ctcgtccacc ggccgcgccg cgcccgtcat ctggcggtgc ctaggccgtc ggtggtgggg
     2281 cggcttccag ctggagctgg gtgtcgatcc agatctgtcc gtccgccttg agggtctcgt
     2341 accagcgtga gtagccggtc gactggatcg tgctcaggcg cgactcctcg accagtcgtt
     2401 cgggattcga gccgagcagc ttgaagatcc agatctggtt gccgtcgacc accgggtcgg
     2461 cgctgatctc gccgatgtcg gtcatgccga agatcgcctg ctcacgcgcc acgcttccct
     2521 cgtagcgagc gacccagccc aggtagccct catcgctccg ggtcgccgag tcctcgctgt
     2581 tggcccgcgc ctcggccccg aacgagtccg gatcggcctc caggtcctcg atcagggagg
     2641 cggcgaagtc cagggcggtc gtgcgctggt ccgtgacctg gatcacgtgg tagccgaagt
     2701 cggtgcggac cggctcgctg atctccccca ccgccaggcg cgccaccgca gcctcgaact
     2761 ccggcacgaa ctggccggtg gtcgggtcat accagccgag gtcgccgccg ttgctccgag
     2821 atccgggatc atcgctctcc ttcgcgatct cgaaccagtc ggcgtccggg tcctggacct
     2881 cgtcgaacca tccctgggca cgggcctcgg ccgcggccca ttgctcctcg gtggccgcct
     2941 cctggtccgc gacctcgccg ccggggaccg gctgggcgag gagatggcgg atccgctgtt
     3001 tcggaaccgg gacgccctgg tcgttgagga tcaggatctg ggcgacctca cgctgctcgg
     3061 caggcgatac ggcgacatcg tcggtgaagt acgtgcggaa atcacgcttc aggagctcgt
     3121 cgccgatgta ggcccggtag tcggcatcgg tgatccgagc ggacgacagg aggtcgagca
     3181 ggcctgtgaa cggcccttcc tcggtccggt cctcgacccg gaggatgacg tagccgtcgt
     3241 cggtcctggt cggcccggac agctcgccgg cctccacccc gtcggccagc gggaacaggt
     3301 agccgtactg cgggtcgtcc tcggagaccc agccgacgag cccctcgaag gtggccgatc
     3361 ccggatcgtc cgattcagcg gtcgcgattg cgccgaactc ctcaccgtcg tcgaggcggg
     3421 cgcggatgtc gttcgcctcc tcctcggcgc gggcaaagtc ctcgtccgtg gcctccgccc
     3481 cgctggccgc gtcctcgggc agggcatcga cctcgatgat cgaaacctgg atccgggcgg
     3541 ggagcgtctg gcgggtcgcc acctcggcat cgatctcctc gtcagtgacc gagatcccga
     3601 actcctccgc gcgcgtggcc tggaacagcc catcggagat cgagccggtg gaggtggcgg
     3661 tgaggttcga gaactggtcg gtgatgaccg agagctgctg ctggagcacg gcgccgcgcg
     3721 cgccgccggt cgtctggtcc tcgcccagct gatcctggat gtcgacgccg gtggcctgca
     3781 gctcggcggc ctggatcgcc ttgcggaggt cgagctcctc gcggattacg gccgtgccct
     3841 ccacgtacat gacctgctgc aggtgcgtgt cgtagtacgc gttccaggcg ctgatcccga
     3901 acaggcccag cgccacggcg atggccgcgg cgaacccgcc caccagcagg cgctgggtcc
     3961 ggagctcgtc ctgccagcgg gcgcgatgac ggcgccgtgg ggccgggcga ttgcgatagg
     4021 tcattggtct cctgtcagcg ctgggcaggc gccatcacgg ttgtgcaggg cgggcgaggg
     4081 cggatcgtac ccgaacggtt catcggtcgc cacgagggcg gcgaacagcc cggatgacgg
     4141 cctgccaccg gagtcggagg acgaggacca ccgcctcggt cacgatcgag cccgacatct
     4201 tcgaccgccc ggccgtgcgc tcgcggaaca ggatcggcac ctcgcggatc gcggcgccgc
     4261 gccggtgcgc ccaccaggtg gtctcgacct ggaagccgta tccctggccg gctgcctcgc
     4321 gaaggcggat cgcctccagc agctcgcggc gccaggcctt gaagccgccg gtcaggtcgc
     4381 ggtagggaag taggagcacg atccgcgcga acagggtgcc gcctcggctg atgatccggc
     4441 gatggattgg ccagcccgag gtcccgccgc cgggcatcca gcgggtgccg agcacgaggt
     4501 cggcgtcccg catgaggggc gcaacgaggc gtggcagatc ggcggggtca tggctgaaat
     4561 cggcgtccat ctggatcacg gccatggtcc cgggcttgtc gagcacccgc ttgaagccgt
     4621 cccggtaggc aacgcccagg ccctccttgc cgggccgatg gaggacctcg acccgcgcgt
     4681 tcgcggcggc gatggtgtcc gccagctccc cggtgccgtc cggcgactga tcgtccacca
     4741 ccaggacctg cgcctcgggc agggcggcga ggacggcctc gaccacgccg acgatgtttt
     4801 cgcgctcgtt gaaggtcggc atcacgatcc acaccccccg gccggcggcg aggcccactg
     4861 acggtgtggg ctcgacagcc tcgatggtca gcgcccgacc cccaggtacc gcacgcccgc
     4921 ggcctcgacc cgcgcccggt ccagcacgtt tcgcccgtcg aggagcacct cgaggccggg
     4981 gaacgcggtc gggtccaggt cgcggtacgc cgtgtgggcc gcctgcagga tgacggcgcg
     5041 gaccggtacc ggcgccgaca ggtcgtacgg cacgaacccc cgccggagca ggtgctcggc
     5101 gtcgaagtac gggtcgtggc cgtggaccgt ggccccggcc gccaccagcg cctcgcgaag
     5161 gagcagcgcg ctgctgaaga agtcctcgcg aacgtctcct cggtagccga tgcccagcac
     5221 gaggacgggc acgccgtcca ggggtccaag cacctcgccc agctggccaa cggcgtacgc
     5281 cgccatctcg tcgttgatcg cccgcgaaag gggcgggatc cgcatctgcg gctcgtcgcg
     5341 gaacaggaga tgcgggtaga ccgggatgca gtggccgccg acgcccaccc ccggttgatg
     5401 gatgtggctg tacggctgcg agttggccgc ggcgatgatc tcggtgacgt cgatcccccg
     5461 ccggtccgcg acctgggcga actcgttggc cagggcaatg ttcacgtcgc ggtaggtcgt
     5521 ttccgccagc ttggtcatct cggccgcctc ggcggaggcc atggccagga tctcggttcc
     5581 cggctcgagg acgtcaccgt agaacgccac cgctcgccga gtcgcctggt cgctcgtgcc
     5641 acccacgatc ttcgggtagc gccgcaggtc gagcaggacc cggcctacca gcacccgctc
     5701 gggcgagaac gcgaggtcga agtcctcggc catccgcagt ccggagccgg cctcgagcat
     5761 cggtcccagc cgttcgcggg tggtcccaat cggcagcgtt gtctcgtaga tgaccatggt
     5821 gccggcctgc agcccatggg ccacgtcgcg ggtggccgcg tcgatctgcg agaagtccac
     5881 ctgccgtgac tcgtcgacca cgaccgggac gatgacgacc acgacctctg cttcgcgcac
     5941 gccggtgctg tcgtcggtcg tggcgcgcag ccgtccggca cggacgagac ccgcgacctg
     6001 gtttgcgacc tcggcctcgt cgtcgtgcgg agactcccct cggttgatcg cctccaccac
     6061 ccgggcgtcg agatcgcagc cgaccacctc gtgaccccgg ctggcgtact ggaccgccag
     6121 cggcaagccg atatgcccga gaccaacgac ggcgatcttc acgccaggat cggggtcagc
     6181 tcgtccgcgc caatgacgac gccccgagcg gccgcgtcga ccatggctcg ggccaggagc
     6241 atggccacca gggcgtcccg tggctcaacc ggcggcggtc ccccgctgcg gatcgcggcc
     6301 gcgaactccg acagctcgac ggccagtggt tcgcggcgct ggaccgcacg gcgtgtcatc
     6361 tcgccctcgc tgaccagctc atggtcaggg ttggcgtaga acaccaggtc ctgggtgagg
     6421 tagtcggcct cgtacatccc gcgttcgccg gtgacggtca gcttgcggac cttggtcggg
     6481 gtcagccagt tgatctcgag caggcccacc gccccggtgt cgaacttgat gagtccggtg
     6541 aacaggtcct cgtgatcggt gtggatccgc tgctcggtct ccgcgtacat ccggatcggc
     6601 tcggcacgga ggaggtagcg catcacgtcc aggtcgtggg gcgcgaggtc gacgacgacg
     6661 ccgacgtcgc gcacgcgcgc ggggaacggc ccgagccggg cggcgcggat ctggaagacg
     6721 cggcccagct cgccggcatg gaggcgacgg cccagctcgc ggat
//
LOCUS       DASYGU010000199         5120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_237615, whole genome shotgun sequence.
ACCESSION   DASYGU010000199 DASYGU010000000
VERSION     DASYGU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237615"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..559
                     /locus_tag="VGO32_05535"
     CDS             107..559
                     /locus_tag="VGO32_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEV7200249.1"
                     /translation="MADSSVAPTEQNPAEPARGRTEPLDPAEAERLFREGVALFNGAR
                     YWHAHEAWETLWRAAPDDERDFYQGLIKVAAGFLHLARRNRRGAKNKLGEGIAHLERY
                     GPVHDGIGVGELVGKAKEVLADLNDGASPYLIGPSIRFVASTSVDRDR"
     gene            556..1944
                     /locus_tag="VGO32_05540"
     CDS             556..1944
                     /locus_tag="VGO32_05540"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV7200250.1"
                     /translation="MTEEATRLEHDSMGEVAVPVGAKWGATTQRAVENFPISGQRLDP
                     SVIHALGRIKAAAASVNGQLGVLDTDAAAAIAAAAEEVAAGGWDDQFPIDVFQTGSGT
                     SSNMNANEVIATLAAEQFGQPVHPNDHVNASQSSNDVIPSAIHLAATDGVVNDLIPAL
                     TELAEALEAKAGEFADVVKSGRTHLMDATPVTLGQEFGGYAAQIRYGIERLEATLPRV
                     GELALGGTAVGTGINAPAEFAPAVITVLRETTGLPLTEARNHFEAQGAQDGLVELSGQ
                     LRTVAIGLYKIATDIRWMGSGPRAGLAEIHVPDLQPGSSIMPGKVNPVVAEALSMVCA
                     QVIGNDAAIAFGGAAGNFELNIMLPMLARNVLESIRLLASSSRVFADRCVRGITADVA
                     RAREFAEGSSAVATALNPVLGYEEAASIAKQALAERKSIRDVVIERGHVDNGNLTLEA
                     LDRLLDVGGMTR"
     gene            complement(1941..2690)
                     /locus_tag="VGO32_05545"
     CDS             complement(1941..2690)
                     /locus_tag="VGO32_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV7200251.1"
                     /translation="MTETTDSESTPPRDLPAREGRHRTVGGNALRAAVLGANDGLVSN
                     FSLVMGVAGAGGDQGAILVAGVAGLLAGAMSMALGEWLSVQSARELYANQLAIEAAEL
                     AEEPEEEENELRLIYEAKGVPTDVARDVAHRIVYGDRAVALETMAREELGIDPKSLGG
                     SAWVAAGTSFALFAMGAIVPVVPFLFLTGTTAIVASAVLAGLALFGVGASITVVTGRG
                     AIRSGLRQLAFGMAAAAITYGIGTLLGTAIS"
     gene            complement(2829..3389)
                     /locus_tag="VGO32_05550"
     CDS             complement(2829..3389)
                     /locus_tag="VGO32_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV7200252.1"
                     /translation="MSTIGHVFVGAAAGRYSLPPTHRIRRLVFFVLLMFIAVAPDLDQ
                     SVLPALGIPDSVTLGHRGATHSLLVAVVTSLLVGLLARACGLPFRVVAIGAFIAIGTH
                     SLLDTFSEGPGVTWLWPFNDTRFPVYPILPSAPANEDLFTRKGLYQLAAEAVVFAPFL
                     LYAIFAKRNPAAEGSPTIDESDRPRA"
     gene            3511..5019
                     /locus_tag="VGO32_05555"
     CDS             3511..5019
                     /locus_tag="VGO32_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7200253.1"
                     /translation="MTATTSTYRNLIAGAWVDARSGRTFTSTSPANRDDVIGEFPASG
                     KDDVDAAVAAAKAAYPAWSMMPAPKRGEILFKVARILTEHKEELSRLLTREMGKVLPE
                     ARGDVQEAIDVAYYMAGEGRRLFGYTVPSEMPDKFAMAIRKPIGVAAIIAPWNFPIAI
                     PAWKLFPALICGNTAVMKPASDTPACMIRFAELLLEGGIPEGVVNVVTGSGTDVGHPL
                     VDHPDVKLISFTGHTETGVDISSRAAKTLKRVSLELGGKNPIVIWDDADIDLALDSVI
                     WSAFGTSGQRCTAASRIVVHRSIHDDFVQKLQKRAAALVLGDGLLETTDVGPVINEKA
                     VERIDGYVAIGQKEAEMVVGGSPARDGELAKGSFFTPTVFAEVKGDARIAQEEIFGPV
                     TAVIPVDGWDEAVQVVNSVQYGLSTSLFTNDVNLAFRSIRDFDSGQGYVNHGTIGAEA
                     HLPFGGTKATGNGHREIGQSALDFYTEWKSVYIDYSGKLQRAQIDMEFVDQD"
BASE COUNT          864 a         1733 c         1759 g          764 t
ORIGIN      
        1 cgtcgggcat tcgctaccgc gccgccatcg agcaggtcct gcgcgagatg cgggtcgccg
       61 ccgcccgcgg ctgatccggc gcttcgggcg ctgtacgatc ggggggatgg ctgactcgag
      121 cgtggcgccg accgagcaga acccggccga accggcacgc ggccgaaccg agccgctcga
      181 tcccgcggag gcggagcgcc tattccgcga gggcgtggct ctcttcaacg gggcgcgcta
      241 ctggcatgcc cacgaggcgt gggaaacgct gtggcgggcg gcgcccgacg atgagcgcga
      301 cttttaccag ggcctcatca aggtggctgc cggcttcctg caccttgccc gtcgcaaccg
      361 gcgtggggcc aagaacaagc tgggcgaggg gatcgctcac ctcgagcggt atgggccggt
      421 ccacgacggg atcggggtcg gcgagctggt gggcaaggcc aaggaggtcc tcgccgatct
      481 caacgatggc gcgtcgccgt atctcatcgg acccagcatc cggttcgtgg cctccacgtc
      541 ggtcgatcga gaccgatgac ggaggaggca acccggctgg agcacgattc catgggcgag
      601 gttgcggtcc cggttggcgc caagtggggg gcgaccaccc agcgggcggt cgagaacttc
      661 ccgatcagcg gccagcggct ggacccatcg gtcatccatg ccctgggccg gatcaaggcc
      721 gcggcggcat cggtcaatgg tcagctgggc gtcctggata ccgatgcggc tgccgccatc
      781 gcggccgccg cggaggaagt tgcggccggc ggctgggacg accagttccc gatcgacgtc
      841 ttccagaccg gctcggggac ctcgtccaac atgaatgcca acgaggtcat cgccaccctc
      901 gccgctgagc agttcggcca gccggtccac cccaacgacc acgtcaacgc ctcgcagtcg
      961 agcaacgacg tcatcccgtc ggccatccac ctcgcggcga ccgatggggt ggtcaacgac
     1021 ctcatccccg cgctgacgga gctggccgag gcactggagg ccaaggccgg tgagttcgcc
     1081 gatgtcgtca agtccggccg aacccacctc atggacgcta cgccggtgac cctgggccag
     1141 gagttcggcg gctatgccgc gcagatccgc tacgggatcg agcggctgga ggccaccctg
     1201 ccccgggtcg gcgagctggc cctcggcggg actgcggtcg ggaccggaat caacgccccg
     1261 gccgagttcg cgccggcggt catcacggtc ctgcgcgaga ccaccggact gccgctgacc
     1321 gaggcccgga accatttcga ggcccagggc gcccaggacg ggctcgtgga gctgagcggg
     1381 cagctgcgga ccgtggccat cggtctgtac aagatcgcga ccgatatccg ctggatgggc
     1441 tcgggcccgc gcgccggcct ggccgagatc cacgtcccgg acctgcagcc cggaagcagc
     1501 atcatgcccg gcaaggtgaa cccggtcgtg gccgaggcgc tgtccatggt ctgcgcccag
     1561 gtcatcggca acgacgcggc catcgcgttc ggcggcgcgg ccggcaactt cgagctgaac
     1621 atcatgctgc ccatgctggc gcggaacgtg ctcgagtcga tccggctgct ggcgtcctct
     1681 tcccgcgtgt tcgcggaccg atgcgtccgc ggcatcaccg cggacgtggc ccgtgcccgg
     1741 gagttcgccg aggggtcatc ggcggtggcg acggccttga acccggtact cggctacgag
     1801 gaggcggcca gcatcgccaa acaggccctg gccgagcgga agtccatccg ggacgtcgtc
     1861 atcgagcgcg ggcacgtcga caacggcaac ctcaccttgg aggccctcga ccgccttctc
     1921 gacgttggag ggatgacccg ctagctgatc gcggtaccga gcagggttcc gatcccatac
     1981 gtgatcgccg ccgcggccat cccgaacgca agctgacgga gaccggagcg aatcgcgccg
     2041 cgtccggtca cgaccgtgat cgaggcgccg acgccgaaca gcgcgagtcc tgcgagcacc
     2101 gcggaggcga cgattgccgt cgtgccagtc aggaacagga atgggacgac gggcacgatg
     2161 gcacccatgg cgaacaaggc aaaggacgtc ccagctgcga cccaggctga cccgccgagg
     2221 ctcttgggat cgatgcccag ctcctcacgc gccatcgtct cgagcgcgac cgcgcggtcc
     2281 ccgtacacga tgcggtgcgc gacgtcccgg gcaacatccg tcgggactcc cttcgcctcg
     2341 tagatgaggc gtagctcgtt ctcctcctcc tccggctcct ccgcgagctc tgcggcctcg
     2401 atggcgagct ggttggcgta cagctcgcgt gcgctctgca cgctgagcca ctctccgagc
     2461 gccatggaca tcgcgccggc cagcagcccg gcgacgccgg ccacaaggat ggctccctgg
     2521 tcaccgccag cgccggcaac gcccatgacc aggctgaagt tgctcacgag cccgtcattt
     2581 gcgccgagga ccgccgcgcg gagcgcattg ccgccaaccg tccggtggcg gccctcgcga
     2641 gcaggcaggt cccgtggtgg agtggactcg gagtcggtcg tttccgtcat ggtcatcagg
     2701 tagcggtcac cttacgtcgc cacgccgcca tcggatctca atcggtctgt ggaaagtgaa
     2761 ggcggtcggt gccacgatcg agcagacgcg atcgatcggg aggatcgcgc tgatcgtccg
     2821 cgccgacatc aggcccgggg ccggtcgctt tcatcgattg ttggtgaccc ttcggcagcg
     2881 gggttgcgct tggcgaagat ggcgtacagc agaaacggcg cgaagacgac ggcctcggcc
     2941 gcgagctgat agagcccttt ccgcgtgaac aggtcctcgt tcgcgggggc cgacggcagg
     3001 atggggtaca ccggaaacct ggtgtcgttg aacggccaca gccacgtaac gccgggcccc
     3061 tcgctgaacg tgtcgagcag cgaatgggtg ccgatcgcga tgaacgcgcc gatggccacc
     3121 acccggaagg gcagcccgca cgcgcgcgcc agcagcccaa cgaggagcga ggtcacgacc
     3181 gccaccagta gggaatgggt ggctccccga tgccccagcg tcaccgagtc cggtattccc
     3241 agggcgggaa gcaccgattg atcgaggtcg ggagccaccg cgatgaacat caggaggacg
     3301 aagaagacga ggcgtcgaat ccgatgcgtt ggcggcaatg aataccggcc ggcggcggca
     3361 ccgacgaaga catgaccgat ggtggacatg agcccggagt ctaccggcgg ccgcgatttc
     3421 tcggtccgac cgggcaattc ccaccgtcac tgcggcctcg cccggacgtg ggacttacaa
     3481 tcggctggac gctcgacgaa cggagatcct gtgacggcta ccacctctac ctaccgaaat
     3541 ctgatcgccg gagcgtgggt tgatgcccgt tcgggcagga ccttcacgtc caccagcccg
     3601 gccaaccggg acgacgtcat cggtgagttc ccggccagtg ggaaggacga cgtcgacgcg
     3661 gcggtcgcgg ccgccaaggc cgcctacccg gcctggtcga tgatgccggc ccccaagcgg
     3721 ggcgagatcc tgttcaaggt cgcgcggatc ctgaccgagc acaaggagga gctgtcgcgg
     3781 ctcctgaccc gggagatggg caaggtgctg cccgaggcgc gcggcgacgt tcaggaagcc
     3841 attgacgtgg cgtactacat ggccggcgag gggcgccgcc tgttcggcta caccgttccg
     3901 tccgagatgc ccgacaagtt cgcgatggcc atccgcaagc cgatcggcgt ggccgccatc
     3961 atcgcgccct ggaacttccc gatcgcgatc ccggcctgga agctcttccc ggcgctgatc
     4021 tgcggcaata cggcggtcat gaagccggca tccgacacgc cggcctgcat gatccgattc
     4081 gccgagctcc tgctcgaggg tgggattccc gagggcgtgg tcaacgtcgt gaccggatcg
     4141 ggcaccgatg tcggccaccc gctggtcgat caccccgacg tgaagctgat ctcgttcacc
     4201 ggccacaccg agaccggggt ggacatctca tcccgcgcgg cgaagacgct caagcgcgtg
     4261 tccctcgagc tgggcggcaa gaaccccatc gtcatctggg acgacgccga catcgacctt
     4321 gccctggact cggtgatctg gtccgccttc ggcacctccg gccaacgctg cacggccgcc
     4381 agccggatcg tggtccatcg gtccattcat gacgacttcg tccagaagct ccagaagcgg
     4441 gcagccgcgc tggtccttgg ggacggactg ctggagacga ccgatgtcgg gccggtgatc
     4501 aacgagaagg cggtcgagcg gatcgacggc tacgtcgcca tcggccagaa ggaagccgag
     4561 atggtcgtcg gtggcagccc ggctcgcgac ggggagctgg ccaagggctc gttcttcacg
     4621 cccacggtgt tcgccgaggt caagggcgac gcgcggatcg cccaggagga gatcttcggc
     4681 ccggtgaccg cggtcatccc ggtggacggc tgggacgagg cggtgcaggt cgtgaacagc
     4741 gtccagtacg gcctgtccac gtcgctgttc acgaacgacg tcaacctcgc cttccgctcg
     4801 attcgcgact tcgactcggg ccaggggtat gtcaaccacg gcaccatcgg cgcggaggcg
     4861 catctaccgt tcggaggaac gaaggcgacc ggcaacggcc accgcgagat cgggcagtcc
     4921 gcactcgact tctacaccga gtggaagagc gtctacatcg actattccgg caagctccag
     4981 cgcgcccaga tcgacatgga gttcgtcgat caggactagg gccatcgccg tcctggcggt
     5041 tgcggggatg gtgatcgcct gttcgccgtc ggcctcggaa cctggttcgg cgactccgtc
     5101 cgcaatcgcg caggcctcgc
//
LOCUS       DASYGU010000200         7266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1112141, whole genome shotgun sequence.
ACCESSION   DASYGU010000200 DASYGU010000000
VERSION     DASYGU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112141"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="dinB"
                     /locus_tag="VGO32_05560"
     CDS             complement(<1..1123)
                     /gene="dinB"
                     /locus_tag="VGO32_05560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV7200254.1"
                     /translation="MRTIIHADMDAFYASVEVHDDPTLVGRPVIVGGRQDDRGVVSAA
                     SYEARAFGVHSAMPLRTAARLCPQAAFLPGRFERYAEVSRQVMAIFDDYTPLVEPISM
                     DEAFLDVSGAPAGDGPTIGRAVRGRVLDELGLVVSVGVATNKLVAKVASDLRKPDALV
                     IVDPGDEAVFLAPLPVTRLWGVGPRVRQALADYGVTTIGQLAAVPDETLRRRFGRMGN
                     ELGQRARGIDPSRVTSGAAPKSIGHEHTFNHDVTERSGLDGSLLWIAESVASRLRRHG
                     LAAGGIQLKLRYEGFETLTRQTHLPHPTTDGRLILDAARRLLDKALEPGRAVRLIGIT
                     AISLANAQQLTLFEDGDRVQRLAEATDAVRDRFGDKAITR"
     gene            complement(1123..1782)
                     /locus_tag="VGO32_05565"
     CDS             complement(1123..1782)
                     /locus_tag="VGO32_05565"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7200255.1"
                     /translation="MASFQRDSAARPRPVSRGNLRPHAVRPAVDWLASPRPAETDPDI
                     AAAHLARRYLAAFGPASVEDLVAYVGRGRNLARWRAVMHAIEDELVTFVDEQGRSLLD
                     LPDAPRPDPDTPATPRLLARWDSLLLAHGTPHRARVLPPAHQTTVITKNADVLPTFLI
                     DGVVAGTWLPRPRGGNGPTIDLRPFGRLKAGDRDALEAEGGRLLPTLGRGAYQRYPGT
                     D"
     gene            complement(1716..2273)
                     /locus_tag="VGO32_05570"
                     /pseudo
     CDS             complement(1716..2273)
                     /locus_tag="VGO32_05570"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
     gene            complement(2319..2981)
                     /gene="lexA"
                     /locus_tag="VGO32_05575"
     CDS             complement(2319..2981)
                     /gene="lexA"
                     /locus_tag="VGO32_05575"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV7200256.1"
                     /translation="MTRHDAERRQRIIEFIARTVTERGYPPSVREIADAVGLASTSAV
                     HHHLVALERAGMLERGGRSSRALRLTAEAEASIASPRPARATITPFRMPLDRDPVRLP
                     LVGEIAAGQPIEAYQDAEPMEVPRAIQAREDSYVLRVRGKSMIDALIDDGDFVIVQPQ
                     ATAHDGDIVVALLEDNGVTLKRFFREDGRIRLQPANAELEPIYATDLQIQGKVVGVIR
                     KL"
     gene            3122..3556
                     /locus_tag="VGO32_05580"
     CDS             3122..3556
                     /locus_tag="VGO32_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEV7200257.1"
                     /translation="MLQLPTYARLVWGLARDSRTPVGLKLLLGGALTYVITPVDLIPD
                     VIPILGHADDLTVLLLVLDLFISNAPIAVREEHMARARTGESTLDEDLAELRLLLGTR
                     FDQIRDSLPSLLERFGGLGDADEVKRMIAERRAHRTKPMGVK"
     gene            3553..3999
                     /gene="rplI"
                     /locus_tag="VGO32_05585"
     CDS             3553..3999
                     /gene="rplI"
                     /locus_tag="VGO32_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV7200258.1"
                     /translation="MKVILKRDVKGLGREGDLKDVKDGYARNHLIPTGAAVLADLGAV
                     RNWDRHKEQREERDRAIRSDAEALGQRIGDLTVQIGVKAGEKDRLFGSVTTRDIADRL
                     RVEGIEVDRHDIHLKEPIKTVGEHQVRIHVMTGIEAQLRVEVVPEH"
     gene            4164..5522
                     /gene="dnaB"
                     /locus_tag="VGO32_05590"
     CDS             4164..5522
                     /gene="dnaB"
                     /locus_tag="VGO32_05590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV7200259.1"
                     /translation="MSIDRVAPQSVEAEQSVLGSILIDPEAILKVADFLHGPDFYRSQ
                     HADIYEAMLALHSQREPIDLVTLGDELTRHDKLEGVGGPAYLATLMNAVPTAVHVEHY
                     GRIVEQKAVLRNLIAAAGKIAAVGYEEAHDTAAAIDRAESILFEISQRRTVAGFETLA
                     TLLGQAYDRLEYLHEHRGQMLGIPTGLAGLDTLLGGFQPSDLVILAARPSVGKTSLAL
                     NVALHAAVHEQKRVAIFSLEMSREQLALRLLSSETGINPRPLQTGFMEETDWSKIARV
                     MNDMASARMWIDDSPTLSVMELRTKARRLEVEQQGLDLVIVDYLQLMQASTPTREPNR
                     VQEVSEISRGLKNLARELKVPVLALSQLSRNVEQRGGVGEPRLSDLRESGSLEQDADV
                     VIFLYRSEGEPNPDADLELVKAKVAKHRNGPIGEVPLQFRRKQTRFYPVEAREEVEVT
                     PY"
     gene            5598..5897
                     /locus_tag="VGO32_05595"
     CDS             5598..5897
                     /locus_tag="VGO32_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV7200260.1"
                     /translation="MFTDKTIACRDCGMDFVFSAGEQQFYEEKGLSHEPQRCPSCRAV
                     AKQNRALGIGTGGGAREMHAAVCAECGGQALVPFLPRNDRPVYCSTCFDKVRARS"
     gene            5970..7145
                     /locus_tag="VGO32_05600"
     CDS             5970..7145
                     /locus_tag="VGO32_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEV7200261.1"
                     /translation="MESGSLLHSPIEVSGRLRRAYGFEEVSLVPGAVTVDPAEVDTSV
                     QIGELTLQIPFLAAAMDAVSDADMAVRLQRHGGLAVVNLEGLYARFDDARPIIEQVVA
                     AADGLAAGRVLADAYDQPIRPDLVRALITRIKASGAAAAVATTPASAWEHAELAADAG
                     ADVFVVQSQVSGAQHISTSGRVLSLADLTGALRMPVVVGNTVSGEATTQLLAQGAAGI
                     LVGIGPGAACTTREVLGIGVPQVSATLEVAAARDRYAAETGRYVPIITDGGMKTGGDI
                     AKAIASGADAVMLGSPFAAALEAPGQGVNWGMAAPSPTLPRGTRITLGERIPLEQILF
                     GPAATTHGTQNLVGALRQSMGALGARTIADMHRVEMVFAPAISTEGKSHQLASHGDR"
BASE COUNT         1129 a         2512 c         2561 g         1064 t
ORIGIN      
        1 ctcgggtgat cgccttgtcg ccgaaccgat cgcggaccgc atcggtcgcc tccgccaggc
       61 gctggacccg atccccatcc tcgaacagcg tgagctgctg ggcgttcgcc agcgagatcg
      121 cagtgatccc gatcaggcgc accgcgcggc ccggctcgag cgccttgtcg agcagccgtc
      181 gcgcggcatc caggatgagc cgcccgtcgg tggtgggatg gggcaggtgg gtctgacggg
      241 tcagcgtctc gaacccttcg tagcggagct tgagctggat cccgcccgcg gccaacccgt
      301 ggcgccggag gcggctggcc accgactcgg cgatccacag cagcgagcca tccagcccgc
      361 tgcgttcggt cacgtcgtgg ttgaacgtgt gctcgtgacc gatggacttg ggcgccgccc
      421 ccgaggtcac ccgggacggg tcgatcccgc gcgcccgctg gccgagctcg ttgcccatcc
      481 gcccgaaccg acggcggagg gtctcgtccg gcaccgccgc cagctgaccg atggtcgtga
      541 ctccgtaatc ggccagcgcc tggcggactc gcgggccgac accccacagt cgggtgaccg
      601 gcaggggggc gagaaacacc gcctcgtcgc cgggatcgac gatgaccaac gcatcgggtt
      661 tgcggaggtc ggacgcgacc ttggccacca gcttgttcgt cgccaccccg acgctgacca
      721 ccagtccaag ctcgtcgagc acccggcctc tcacggcccg gccgatggtc ggtccgtcgc
      781 cggccggcgc gccgctgaca tcgagaaagg cctcgtccat gctgatcggc tccaccagcg
      841 gggtgtagtc gtcgaagatg gccatcacct ggcggctgac ctccgcgtag cgctcgaagc
      901 ggccgggcaa gaaggcggcc tgggggcaga gccgggcggc ggtgcggagc ggcatcgcgg
      961 agtggacgcc gaacgctcgt gcttcgtacg acgccgcgct gaccacgccc cggtcgtcct
     1021 ggcggccgcc gacgatcact ggccggccga cgagggtcgg gtcgtcgtgt acctcgaccg
     1081 acgcgtagaa cgcgtccatg tcggcgtgga tgatggtccg catcaatcgg tgcccggata
     1141 tcgctggtac gcgcctcggc cgagggtggg gagcaggcgc ccaccctccg cctcgagcgc
     1201 atcgcgatcc ccggccttca gccgaccgaa tggccgcagg tcgatcgtgg ggccgttgcc
     1261 accgcgcggc cttggaagcc aggtgccggc cacgacgcca tcgatcagga aggttgggag
     1321 cacatcggcg ttcttcgtga tcacggtcgt ttggtgggcc ggcggcagga cccgggcgcg
     1381 atgaggggtg ccgtgggcca ggagcaggct gtcccatcgc gcgaggagcc ggggagtggc
     1441 aggggtgtcc gggtcgggac gcggggcgtc cggcaggtcg agcaggctgc ggccctgctc
     1501 gtcgacgaag gtgaccagct cgtcctcgat ggcatgcatc accgctcgcc accgggcgag
     1561 gttccggccc cgaccgacgt aggccacgag gtcctcgacg ctcgccggtc cgaacgccgc
     1621 gaggtagcgc cgggcgaggt gggcagccgc gatgtcggga tcagtctcgg ccggccgcgg
     1681 tgacgcgagc cagtcgacag ccggacgtac cgcgtgcggc cgtaagtttc ctcgggatac
     1741 gggacgtgga cgagcggcac tgtcccgctg aaatgacgcc acagcggccg gttgggcggg
     1801 atctcggtcc ggcccatctc tgccttcagg atggcctcga tctcatgaat cgccagcggc
     1861 cgatcggcca tcgcggccag gaccgcagcg tggccccggg tgatccgctt cccgagcggc
     1921 gagctgaccg ggtccgcctt gaacaggagc cgccactgtt cgaggcggaa tggatgggtg
     1981 agcatggaca tgggccagaa gtcggcggcg ctgacgacat ggaccgtcat gcgcatgagc
     2041 gacgcgcgga cgatggactt cccggcccgc gcccggacga ggtccaccgg ctgggatcgg
     2101 gcggccatcc gggactggag cgcaagcggc ggccagtccg gatgctgggc ctggagcgac
     2161 ccggcgcgct cgacgagacg ggccacctcg tcagtcccga atggctggag gagaccctgg
     2221 cgggccaggg tcgcccggtt cagcgcgcga ttggtcagaa gcggcggaga catcggtcag
     2281 gctccagcct agcggcggat gccgcccggt tcaggtggtc agagcttgcg gatgacgccg
     2341 acgaccttgc cctggatctg gaggtcggtg gcgtagatgg gctcgagctc ggcgttggcc
     2401 ggctgcagac gaatccgccc gtcttcgcgg aagaaccgct tgagggtgac cccgttgtcc
     2461 tccagcaggg cgacgacgat gtcgccgtcg tgcgcggtgg cctgcggctg gacgatgacg
     2521 aagtccccat cgtcgatgag ggcatcgatc atgctcttgc ctcggacgcg gaggacgtat
     2581 gagtcctcgc gggcctggat ggcgcgtggc acctccatgg gctccgcatc ctggtaggcc
     2641 tcgatgggct gcccggccgc gatctcgccg accaacggca gacgcaccgg atcccggtcg
     2701 agcggcatgc ggaagggcgt gatggtggcc cgcgccggcc ggggggaggc gatcgaggcc
     2761 tccgcctccg cggtgaggcg cagcgcccgc gaggagcgcc ccccgcgctc gagcatgccg
     2821 gcccgctcga gggcgacgag atggtggtgg accgcggacg tcgaggccag gcccaccgcg
     2881 tccgcaatct cgcgaaccga cgggggatac ccccgctcgg tcacggtgcg cgcgatgaac
     2941 tcgatgatgc gctgtcgccg ttcggcgtcg tgccgggtca cggaacgagc ctagcagaac
     3001 acatgttcta ttgtcaacgc ctctgctatg atccgcgccc acccgaaacc tgcctcggac
     3061 ggagaccgaa ccgatcgtga tgcgtcccgg ccggtggcgc tatctgcgca ccgccagact
     3121 catgctccag ctgccgacct atgcccgcct ggtatggggt ctggctcgcg actcgcgcac
     3181 gcctgttggc ctgaagctgc tcctcggcgg ggcattgacg tacgtcatca cgccggtgga
     3241 cctcatcccc gacgtcatcc ccatcctcgg ccacgccgac gacctcaccg tcctgctcct
     3301 ggtcctggac ctgttcatca gcaacgcccc gatcgccgtc cgcgaggagc acatggcgcg
     3361 ggcgcgaacc ggggagtcga ccctcgacga ggacctggcc gagctccggc tcctgctcgg
     3421 gacacgcttc gaccagatcc gggactcgct gccgtcgctc ctcgagcgct tcggtggcct
     3481 gggggacgcg gacgaggtga agcgcatgat tgcggagcgg cgggcgcacc gaacgaagcc
     3541 tatgggagtg aaatgaaggt catcctcaag cgtgacgtga agggcctcgg ccgtgagggc
     3601 gacctcaagg acgtgaagga cggctacgcc cgcaaccacc tcattccaac cggagccgcg
     3661 gttctggccg acctcggcgc cgtgcgcaac tgggaccggc acaaggagca gcgcgaagag
     3721 cgcgaccgcg cgatccgatc cgatgccgaa gcccttggcc agcgcatcgg cgatctcacc
     3781 gttcagatcg gggtcaaggc cggcgagaag gatcggctgt tcgggtcggt gaccacccgc
     3841 gacatcgccg accggctgcg cgtggaggga atcgaggtcg accgccacga catccacctc
     3901 aaggagccga tcaagaccgt cggtgagcac caggtgcgga tccacgtcat gaccgggatc
     3961 gaggcccagc tccgggtcga ggtcgtcccc gagcactgac ccgatcgcgc gacacgcaag
     4021 ctgtcgtgag gttcgcgtcc catccggggc gtgttctcca cagtcgacgg acaggttgac
     4081 gccgcttatc cccgacgccg tttgaaagcg gtggacaagg ccattcccgc ccggcctccg
     4141 ggtccgtagc gtcactcgag ccgatgagca ttgaccgcgt cgccccccag tcggtcgaag
     4201 ccgagcagag tgtgctcggc tcgatcctga tcgatcccga ggccatcctc aaagtcgccg
     4261 acttcctcca cgggcccgat ttctatcggt cccagcacgc ggacatctac gaggccatgc
     4321 tggccctcca ctcccagcgg gagcccatcg acctcgtcac cctcggcgac gagctgaccc
     4381 ggcacgacaa gctggaggga gtcggcggcc cggcgtatct cgcgaccctc atgaacgccg
     4441 tgcccaccgc ggttcacgtc gagcactacg ggcggatcgt cgagcagaag gccgtcctga
     4501 ggaacctgat cgcggccgcc ggcaagatcg cggcggtggg gtacgaggag gcgcacgaca
     4561 cggcggccgc catcgatcgg gcggagtcca tcctgttcga gatcagccag cggcgaacgg
     4621 tcgccggctt cgagacgctg gccaccctgc tcggccaggc gtacgaccgg ctcgaatacc
     4681 tccacgagca tcgcggccag atgctcggca tcccgaccgg cctggccggg ctggacaccc
     4741 tgctcggcgg tttccagccc tcggacctcg tcatcctcgc cgcccgaccc agcgtcggaa
     4801 agacgagcct tgccctgaac gtggccctcc atgccgcggt ccacgagcag aagcgggtcg
     4861 ccatcttctc gctcgagatg agccgcgagc agctggccct ccgcctcctg tcatccgaga
     4921 ccggcatcaa cccgcgaccg ctgcagaccg ggttcatgga ggagaccgac tggtccaaga
     4981 tcgcccgggt catgaacgac atggcgagcg cccggatgtg gatcgacgac tcacccaccc
     5041 tgtcggtcat ggagctgcgg accaaggctc gccggctgga ggtcgagcag cagggcctgg
     5101 acctcgtgat cgtggactac ctgcaactga tgcaggcctc gaccccaacc cgcgagccga
     5161 accgggtcca ggaggtgagc gagatcagcc gtggcctgaa gaacctcgcc cgcgagctca
     5221 aggtgccggt gctggcgctc tcgcagctgt cgcgcaacgt ggagcagcga ggcggcgtcg
     5281 gcgagccgcg gctgtcggac ctgcgcgagt ccggttcgct cgagcaggac gcggacgtgg
     5341 tcatcttcct gtaccggagc gaaggcgagc ccaaccccga tgcggacctc gagctggtca
     5401 aggccaaggt cgccaagcac cggaacggac cgatcggcga agtgcctctc cagttccgac
     5461 ggaagcagac ccggttctac ccggtggagg cccgcgaaga ggtcgaagtc accccgtatt
     5521 aagggggttc cccacgctcg tggcgcatgg catgatcggc cgctgggacg tcccgtaccc
     5581 acggagaggg agggtcagtg ttcaccgaca agaccattgc gtgccgtgac tgcggcatgg
     5641 acttcgtctt ctcggccggc gagcagcagt tctacgagga gaaggggctg tcccacgagc
     5701 cccagcgctg tccgagctgt cgggcggtgg ccaagcagaa ccgggcgctc gggatcggta
     5761 ctggaggggg cgcgcgggag atgcacgcgg cggtctgtgc cgagtgcggt ggccaggcgc
     5821 tcgttccgtt cctgccccgc aacgaccggc cggtctactg cagcacctgc ttcgacaagg
     5881 tgcgcgcccg ctcctgacct cgacctgacc cgtcgcggcg ccgcctatac tgcggcccca
     5941 cccggctgac cgtaccggag gcttccccga tggagtccgg ctcgctcctc cattccccga
     6001 tcgaggtcag cggtcgcctc cggcgcgcat acgggttcga ggaggtctcg ctcgttccgg
     6061 gcgcggtcac ggtggacccg gccgaggtag acacctccgt ccagatcggt gagctgaccc
     6121 tccagatccc gttcctggcc gcggccatgg atgccgtgag cgacgccgac atggccgtgc
     6181 gcctccagcg ccacggcggg ctggcggtgg tcaacctcga gggcctgtac gcgcggttcg
     6241 acgacgccag accgatcatc gagcaggtcg tggcggcggc cgacggcctg gccgcgggtc
     6301 gtgtcctggc cgacgcctac gaccagccga tccgcccgga cctggtccgg gcgctcatca
     6361 cccggatcaa ggcctccggg gccgcggccg ccgtggcgac cacgccggca tcggcctggg
     6421 agcacgccga gctggccgcc gatgcgggcg ccgacgtctt cgtcgtccag tcccaggtct
     6481 cgggcgccca gcacatctcg acctcgggcc gggtcctgtc cctggccgat ttgaccggcg
     6541 ccctgcggat gccggtcgtg gtcggcaaca cggtgagtgg cgaggccacc acccagctgc
     6601 tggcccaggg cgcggcggga atcctggtcg ggatcggacc cggggcggca tgcacgactc
     6661 gcgaggtgct gggcatcggc gtgccccagg tgtccgccac gctcgaggtg gccgctgccc
     6721 gggatcggta cgcggccgag accggtcgtt acgtcccgat catcaccgac ggcgggatga
     6781 agactggcgg tgacatcgcc aaggccattg cctccggcgc ggacgcggtg atgctcggat
     6841 cgccgttcgc ggccgccctc gaggcacccg gccagggcgt gaactggggg atggcggcgc
     6901 cttctcccac actcccgcgc gggacccgga tcacgctcgg tgagcgtatc ccgctggagc
     6961 agatcctctt cggtccggcg gccaccaccc acgggacgca gaacctggtc ggggccctgc
     7021 gccagtcgat gggcgcgctg ggggcacgga cgatcgccga catgcacagg gtcgagatgg
     7081 tgttcgcacc cgccatttcc accgagggca agtcccacca gctggcgagc cacggagacc
     7141 ggtgaaggtc atccgagccg ccgaccgccg cttcgagccg gcggggaccg agacgttcag
     7201 cggcgaggtg tggatgggga cgaacttcgc cgcggacggc ccgggaacga tgaattcggc
     7261 cgaggt
//
LOCUS       DASYGU010000201         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_804327, whole genome shotgun sequence.
ACCESSION   DASYGU010000201 DASYGU010000000
VERSION     DASYGU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804327"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..862
                     /locus_tag="VGO32_05605"
     CDS             89..862
                     /locus_tag="VGO32_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200262.1"
                     /translation="MTAAVSHSGAIAARHIRTFRRQPWWIAISLMQPVIYLVLFGSLF
                     AELANGTAFGGSYIDFLLPGIVVMSALSSGGWAGTASIEDIHGGVMDRMLVTPVRRAA
                     VIIGHLGQQALIVTLQAVVLIGLGLLIGARFDGGIAGVAVMLGASILLGTAIGALSHA
                     IALLARTQETLIAVSQGIILPMTFLSTTFMVTALMPDWMATVATYNPLTWAVDAARAA
                     VDGSGDPAFIVARLAWLGIFLVVSLVLALQAFGRYRRTA"
     gene            934..>2939
                     /locus_tag="VGO32_05610"
     CDS             934..>2939
                     /locus_tag="VGO32_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7200263.1"
                     /translation="MPEESPETAFERRIVSVLFADLVGFTSLSEQLDPEDVATVQDAY
                     FATVRETVARHGGVLEKFIGDAAMAVFGVPKARDDDAERAIRAGLALVGAVELLGARL
                     GLATGDAGDAGLQLRVGVNSGEVVSAESGPDAGRVTGDTVNLAARFQAAADPGTVMVG
                     ELTQLSAADAVEFESIGALELRGKAEPVPAWRVQRLRPERSREEALGNLRAPLLGRAD
                     ELARLESVLNRVAERREGGSEVIVAPPGVGKSRLLTEFESRAKEAGATVLRARVRADV
                     LGPTEPVGQLLLAALRTLDGASREAVPEALDRALNGAGASPGRAAVVTAEVIETLWPP
                     PVVAGAVPPDRDARFGAWLEALDALAGEQLSAWLIEDIHWAGGDLLAFLALAGTWPAR
                     AGRMVVATARPSVLEHLAEGTSVLELPPLPALEASELIRALVGDALPDAVVTAVAERS
                     DGNPLFIEELLRSWISSGTLVADGEGWRLAVAPEAVALPATVQAIYAAQLDDLPAPAR
                     LIARRASVAGRRFAGRALEPLDVPEPAEGLERLRQRAFLRGPESDMTGDDIYAYRHAL
                     LRDAGYASLSRAERGRLHLALAIWLEQTAGTDVARVAEPIASHYEAALAALPGVGGDP
                     DERLRLTDAARSWLERAADAALAVAGHDAAIGLLGRAADLAAGD"
BASE COUNT          378 a         1048 c         1065 g          448 t
ORIGIN      
        1 tctctcgtcc gtcgctggac gacgtctatc tccgccacac cggccgatcg ttcgcatcgg
       61 ccgagcgaac cctccaggag gcaacccgat gactgctgcc gtctcccaca gcggggccat
      121 tgcggcccgc cacatccgca ccttccgccg ccagccgtgg tggatcgcca tcagcctcat
      181 gcagccggtg atctacctcg tcctgttcgg ctccctgttt gcggagctgg ccaacgggac
      241 cgccttcggc ggctcgtaca tcgacttcct gctgcccggg atcgtggtca tgagcgccct
      301 gtccagcggg ggctgggcgg gcaccgcctc catcgaggac atccacggcg gggtcatgga
      361 ccgcatgctc gtgacgccgg tgcgccgggc ggcggtcatc atcggtcacc tcggccagca
      421 ggccctgatc gtgaccctcc aggcggtggt cctcatcggc ctgggcctgc tcatcggcgc
      481 ccgcttcgac gggggcatcg ccggcgtggc ggtcatgctg ggcgcgtcca tcctgctggg
      541 aaccgcgatc ggggccctgt cgcatgcgat tgcgctgctg gcccgcaccc aggaaacgct
      601 gatcgcggtc agccagggaa tcatcctgcc catgaccttc ctgtcgacca cgttcatggt
      661 caccgcgctg atgccggact ggatggccac cgtggccacc tacaacccgc tcacctgggc
      721 cgtggatgcg gcccgggcgg cggtggacgg cagcggcgac cccgcgttca tcgtggcccg
      781 cctggcctgg ctggggatct tcctggtcgt gagcctcgtc ctggcgctcc aggccttcgg
      841 ccgctaccgc cggaccgcct gagtccgtcg gacgaccgag agcggcgggc gatcttgccc
      901 gccggtccgg tgcggctcgg atagagtccg agggtgcccg aggaatcgcc cgagaccgcg
      961 ttcgagcggc gaattgtcag cgtcctgttc gcggacctgg tcggcttcac gtcgctgtcg
     1021 gagcagctgg atcccgagga cgtggccacc gtccaggacg cgtacttcgc gaccgtccgt
     1081 gagacggtgg cgcgccacgg aggcgtgctc gagaagttca tcggcgacgc cgcgatggcc
     1141 gtcttcgggg tgcccaaggc tcgcgacgat gatgcggaac gggccatccg cgctgggctg
     1201 gcgctcgtcg gagcggtgga gctgctcggc gcgcggctcg gactcgcgac cggtgacgcc
     1261 ggcgatgccg ggctgcaact gcgcgtgggc gtcaactccg gggaggtggt ctcggccgag
     1321 agcggccccg acgcgggacg agtaaccggc gacacggtca acctggccgc ccgcttccag
     1381 gccgccgccg atcccggaac ggtgatggtc ggcgagctga cccagttgag cgctgccgat
     1441 gcggtcgagt tcgaatcgat tggcgcactg gagctgaggg gcaaggcaga accggtgccc
     1501 gcctggcgag tgcaacgcct ccgtccggag cgatcgcgcg aggaagcgct gggcaacctc
     1561 cgtgctcccc tgctcggccg ggccgacgag ctggcgcggc tggagagtgt cctgaatcgg
     1621 gtcgccgagc gccgagaggg tgggagcgag gtcatcgtcg cgcccccggg cgtgggcaag
     1681 agccggttgc tgaccgagtt cgagagtcgc gcgaaggagg ctggcgcaac cgtccttcga
     1741 gcgcgggtgc gggccgacgt gctcggcccg accgagccgg tgggccagct tctcctcgcc
     1801 gccctgcgca cgcttgatgg cgcgagccgc gaggcggtgc cggaggccct ggatagggca
     1861 ttgaacggcg ccggcgcgag tcccggtcgg gcggcagtcg tgaccgccga ggtcatcgag
     1921 accctgtggc cgccacccgt cgtcgccggc gcggtacctc ctgatcggga tgcgcgcttc
     1981 ggggcgtggc tcgaggctct ggacgcgctc gcgggagagc agctcagcgc ctggctgatc
     2041 gaggacatcc actgggccgg tggcgacctg ctggcattcc tggccctggc cggaacatgg
     2101 cccgcacggg ccggtcggat ggtcgtggcg acggcccgcc catcggtcct cgagcacctg
     2161 gccgagggca catcggtcct cgagctgccg ccgctgccgg ccctcgaagc cagcgagctg
     2221 atccgggcgc tggtcggcga cgccctgccg gacgcggtcg tgacagcggt agccgagcgg
     2281 tcagacggca acccgctgtt catcgaagag ctgctgcgaa gctggatcag cagcggcaca
     2341 cttgtggccg acggcgaggg ctggcgtctg gcagtcgccc ccgaggcggt ggcccttccc
     2401 gccaccgtgc aggccatcta cgccgcccag ctggatgacc tccccgctcc cgcccgcctg
     2461 atcgcccgcc gcgcgtcggt ggccggtcgc cggttcgcgg gccgcgcgct cgagccgctc
     2521 gacgtgcccg aaccggccga aggcctggag cggcttcgtc aacgggcgtt tctccgtgga
     2581 cccgagtcgg acatgaccgg cgacgacatc tacgcctacc gccatgccct ccttcgtgac
     2641 gccggctacg cgagcctgtc acgcgccgag cgcggccgtc tccaccttgc gctggcgatc
     2701 tggctggagc agacggcggg aaccgatgtc gcccgggtcg ccgagccgat cgcctcccac
     2761 tacgaggcgg ctctcgcggc cctccccggt gtaggcgggg atccggacga acgcctccgc
     2821 ctgaccgatg ctgctcgctc ctggctggag cgggcagccg acgccgccct ggccgtcgcg
     2881 gggcacgacg cggcgatcgg gttgctcggg cgagccgcgg acctggccgc cggcgacga
//
LOCUS       DASYGU010000202        10383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_186886, whole genome shotgun sequence.
ACCESSION   DASYGU010000202 DASYGU010000000
VERSION     DASYGU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186886"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGO32_05615"
     CDS             <1..822
                     /locus_tag="VGO32_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7200264.1"
                     /translation="DAPALKAADIGVAMGITGTEVSKEAANMILTDDNFATIVKAVEL
                     GRGLYDNLKKYIRFQIGVLIGYITLFLGSSILNVLGGVPFVPFQTLFMNFTIQIFQAV
                     GLGYGKPAEGLMERKPRPSNEPLLSRKLLAWLAIAGVVMGGGTLAVNAWAANEYNDTV
                     SRTMGMVVFSLSNVAFSLATKDERISVFSLDLLGDRPFLLGTLASLVTIVLMAELDFF
                     NRILETTGLTLDQWAICLVVGLLIIPVSEVRKRIWDVEEDEALVEPAPAQAAAAS"
     gene            complement(829..903)
                     /locus_tag="VGO32_05620"
     tRNA            complement(829..903)
                     /locus_tag="VGO32_05620"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Gln,seq:ctg)
     gene            1088..1429
                     /locus_tag="VGO32_05625"
     CDS             1088..1429
                     /locus_tag="VGO32_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200265.1"
                     /translation="MSTSVEPKTWRRYGFGGPPEPWPRDEQRDLDRLATSYYVDVLEF
                     RRLALAEDQPPPEVEELFMLATRHKQELDYTLRYWATPAERARAEDRLGSLMRIARRL
                     HDIAERVPGPA"
     gene            1439..2425
                     /locus_tag="VGO32_05630"
     CDS             1439..2425
                     /locus_tag="VGO32_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV7200266.1"
                     /translation="MAAPPLPTALDILRARRRIGDAVSRTPLLASLPLAKAARAAEVR
                     LKLENLQPAGSFKVRGAESAVAVIAAVDSTSHLITASSGNHGIAIARAAARHGLPVTI
                     LVGGSVSPAKLERLRELETDRCTVEIFGRDTDDTEPEARRRQALGAGIYVAPYNDPNV
                     VAGQGTVGVEILEEWPDVDTIVVPVGGGGLIGGIGLWARTINPAIRIVGVQPTASPPM
                     QDFLATGRARRVPIGPTLADGVAGNIERGSITFRLAKRYVSELVLVDERAIADAMRWA
                     WEGPHHRLEGSAALGIAALHTGKLGALADHRVAVVLSGGNVDASTFDAVVGG"
     gene            2435..2980
                     /locus_tag="VGO32_05635"
     CDS             2435..2980
                     /locus_tag="VGO32_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200267.1"
                     /translation="MTANRQPPPGVILGGVLIVIGLSVLAVRYLDVFAGESIWPLFII
                     GPGLLMIVLGLILPNVPMLVGGCVVATIGGILAWQNQTGLWATWAWIWALIPIASGVG
                     SLLGGIRTGDREARNSGLWQIVIGVALLAVFFLFFEQFIGLSGGEIPVPEWAMPAVLM
                     GLGVLILLRDFFERPDPDPTT"
     gene            2977..3576
                     /locus_tag="VGO32_05640"
     CDS             2977..3576
                     /locus_tag="VGO32_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200268.1"
                     /translation="MTGGPVEVRPLTAKRWPDLVELFGPSGAFSNCWCTWWRQTGGEF
                     GRGIAQRGAGNKALLGELTHANRQPGLVAYRDDRPVGWISVAPRPDYGRVIRSPSIGP
                     GRRSAEAADEGVWSVVCFWMPRKERGKGIAMALLRAAVDHARAGGGRVLEGYPVDTAG
                     ERHPQSSVFTGTLAMFRRVGFTEVERRAEGRPIVRLDLS"
     gene            3634..7230
                     /locus_tag="VGO32_05645"
     CDS             3634..7230
                     /locus_tag="VGO32_05645"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEV7200269.1"
                     /translation="MPADDTNGRSPLDEALEGLNAGYVAAVYEQYQSDPASVETEWRR
                     LFDAGYGGFEPVRTPSPAAPADPSDPPAGARPLKGPAARLASNMAASLAVPTATSFRD
                     VPVAVLEARRQQLNEAIAPRKVSFTHLVGYALARAAGEQPGMTRAFTEVNGAAHVVES
                     GAVNLGLAVDVERPDGSRFLVVPVIRAADDVDFAAFHGRYEELVAGARDSTLGPDDYA
                     DATMTLTNPGTLGTTASVPRLMVGQGCIVATGAIRDTGSGRVMTITSTYDHRVIQGAE
                     SGAFLRRVADLLGGADGFYESVTASLGVTPLPDLPVAAAAAPAPSSSPVPPTPAGPSV
                     TVSGADLGRMASAMALIDAYRLYGHLAAQLDPLGTPPPGDPALDPAFHGLDPDAAGRI
                     SADLLKVHVGGDTLAEVLVRLGRTYSGTIAYEVEHIANHAERTWLREAIESGRFRVAL
                     KRKEQKRLLSRLTAVEGLEHFLHKAYLGQKRFSIEGLDATVPMLDLVLEMAADSGTKR
                     AVIGMAHRGRLNVLAHIVGVPMRSILAEFEAGKGGRAPSEGLEIDDVKYHLGASGKNE
                     TRRGTVDVTLMPNPSHLEAVNPVVEGRARAEQTDHRGPLVKIDTRRTVPILLHGDAAF
                     AAQGVVAETFNLSRLDGYANGGTVHLIMNNQIGFTTSVREARSTTWASDLAKGFDVPI
                     IHVNADDPEACLDAVRLAMAYRAEFNGDVVIDVVGYRRYGHNEADEPSYTQPGMYDRI
                     ARQPSVRALYADRLAADDVVSAKQGAEMADEVRRDLIAAQASVRKAHSEPPLDRGEDA
                     LATESPDEPATGVGMDVLLALNDQLHATPKGFTVHPKLVRQLTRRREALGIDEPGIEW
                     GHAESMAFATLVHDGVPIRLTGQDTVRGTFSQRHQVLVDAATDATWTPMQHIDGAKAS
                     FEVHNSPLSEYACLAFEYGYAVTVPDALVLWEAQFGDFVNGAEIVVDQFIIAGLAKWR
                     QTSRLTLLLPHGYEGQGPEHSSARLERFLALGAEGNIRVAYPTTSAQYFHLLRWQGLH
                     EELRPLVVMTPKSLLRLPEAASRPSELAGGSWAPVIDDPERQTDAAAGAVRRVILCSG
                     KIYYDLAMSEGRAAAADVALIRVEQLYPFPTETLATVLSRYRRMRRVSWVQEEPRNMG
                     ARKFVLPKIRSIVAARIPLGDVSRPERSRPAEGYPAAHAAEQARIVRDAFAD"
     gene            7249..8322
                     /gene="thrC"
                     /locus_tag="VGO32_05650"
     CDS             7249..8322
                     /gene="thrC"
                     /locus_tag="VGO32_05650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV7200270.1"
                     /translation="MTDLTTARSPSALLERYADLLARPGESFPISLGEGATPLIRARR
                     LGAAVGVDRLFLKFEGTNPTGSFKDRGMVLAVNRAVAAEARMVVCASTGNTSASAAAY
                     AAAAGLRCGVVLPAGKVARGKLAQALAAGARLVLTDGNFDAALEAVRELGQSGEAVVV
                     NSINPDRLEGQQTVAWEICDTLGAAPDAVALPVGNAGNITAAWRGFVAYAEAGRADAR
                     PRMLGFQAAGAAPIVNGAAVADPETVATAIRIGNPASWAGATVARDESGGLIEAVTDE
                     QILAAQADIARLEGVFCEPASAAGVAGVRQLAASGRLERSATVVCILTGHGLKDPDTA
                     PGHAGDLAPVPATADAIRAALDG"
     gene            8315..8887
                     /locus_tag="VGO32_05655"
     CDS             8315..8887
                     /locus_tag="VGO32_05655"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV7200271.1"
                     /translation="MADLARPRCQWVADAGDAMLAYHDDEWGVPIHDDRALFELLTLE
                     GAQAGLSWSTILNRRDGYRRAFAGFDIVAVAAFGSDDQARLKADDGIIRNAAKIRSAI
                     GNARLTLVLGNLDAHLWSFVGGRPIQNAWRGHDVPAETAESKAMSRDLKRRGFSFVGP
                     TICYAFMQSAGLVNDHALLCFRHDEVARLA"
     gene            8905..9837
                     /gene="thrB"
                     /locus_tag="VGO32_05660"
     CDS             8905..9837
                     /gene="thrB"
                     /locus_tag="VGO32_05660"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV7200272.1"
                     /translation="MATPIASVDVPASTANLGSGFDCFAAALSLKLRAELHHGNLPGI
                     VLNAHGEGAIEAESAGSDSMVVRAFREGLRAAGGPPGGSASWRIEVSSMIPAARGLGS
                     SAAAILAGILLGAAAGRLPTNADQLLALAARLEGHPDNVSAALYGGFTLSIMEAGDVT
                     VRRFRVPEAWIPVLFIAQTESRTEDMRAALPGRVPLPDAANQAARAALLATAVLTSDA
                     SLLRRAMDDRLHQPYRLPLLPGSARLIETAYQIGAAGACLSGAGPSVLAICDSPLTAH
                     SVEKAWNGSGQDGMATRLRFDTAGAKLNAAEARE"
     gene            9848..>10383
                     /locus_tag="VGO32_05665"
     CDS             9848..>10383
                     /locus_tag="VGO32_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7200273.1"
                     /translation="MSLVSLSGVSKSHGAQHLFSDVALQVSAGRRVAIVGANGTGKTT
                     LLEIITGEQDADTGVVARGKDVVIGYLRQEVAESRGHSVLAEVLAGAGTVTGIGRRMQ
                     HIEQEMAEAIAEDELAELMDEYGRLQHRFEQLGGWSIESEARRILAGLGFAEADAERD
                     IGEFSGGWMMRVALARLLL"
BASE COUNT         1538 a         3693 c         3606 g         1546 t
ORIGIN      
        1 gacgccccgg ccctgaaggc cgccgacatc ggggtggcga tggggatcac cggcacggag
       61 gtctccaagg aggccgccaa catgatcctg accgatgaca acttcgcgac catcgtgaag
      121 gcggtcgagc tcggtcgcgg cctgtacgac aacttgaaga agtacatccg cttccagatc
      181 ggtgtcctga tcggctatat caccctgttc ctggggtcct cgatcctcaa cgtcctcggt
      241 ggcgtgccgt tcgtgccgtt ccagaccctg ttcatgaact tcacgatcca gatcttccag
      301 gcggttggcc tgggctacgg caagccggcc gaggggctca tggagcgcaa gccacggccg
      361 tccaacgagc cactcctgtc tcggaagctc ctggcgtggc tcgcgatcgc cggcgtggtg
      421 atgggcggtg ggacgctggc ggtcaacgcc tgggcagcca acgagtacaa cgacaccgtg
      481 tcccggacga tgggcatggt cgtgttcagc ctctcgaacg tcgcgttctc gctggccacc
      541 aaggacgagc gaatatccgt gttcagcctc gacctactgg gagaccggcc gttcctcctc
      601 ggcaccctcg cctcgctcgt caccatcgtg ctgatggcgg agcttgactt cttcaaccgc
      661 atcctggaaa ccaccggcct gacgctcgac cagtgggcca tctgcctcgt ggtcggcctg
      721 ctgatcatcc cggtctccga ggtccgcaag cggatctggg acgtcgagga ggacgaggcg
      781 ctcgtcgagc cagcaccggc gcaggccgcc gcggcgtcat aggggagttg gctggggcgg
      841 aaggattcga accttcgatc tcctgatcca gagtcaggcg ccttaccact tggccacacc
      901 ccagcggggc cgagcgattc taactcaggg cctcccgcgt ccccgatagg gccatgcggg
      961 tccggggcgc cgatcgtccc gtgactcgta gcccgagagg gcggctacgc cgtgccgacg
     1021 ccccccagta cggtcaggac gctgaactga agccgccata aatagaggaa aggggagtcc
     1081 tgcaaccatg tcgacctccg tggagcccaa gacatggcgc cgctatggat tcgggggtcc
     1141 ccccgagccc tggccgcgtg acgagcaacg agatctggat cggctggcaa cgtcgtacta
     1201 cgtggatgtc ctggagttcc ggcgtcttgc cctggccgaa gaccaaccgc cgcccgaggt
     1261 cgaggagctc ttcatgctcg cgacccggca caagcaggag ctcgactaca cgctccggta
     1321 ctgggccacc cccgccgagc gtgctcgtgc cgaagaccgg cttggatcgc tgatgcggat
     1381 cgcccgccgg ctgcacgaca tcgccgagag ggttccaggg cccgcataga ctgcggaggt
     1441 ggcagcacct ccgctcccga cggccctgga tatcctccgc gcgcgacgtc gcatcggcga
     1501 cgccgtcagc cgaacaccgc tcctcgcctc gctgcccctc gccaaggcgg cgcgcgcggc
     1561 cgaggtccgg ctcaagctcg agaacctcca acccgctggc tcgttcaagg tccgcggcgc
     1621 ggagtcggcg gtggccgtca ttgcggccgt ggacagcacc tcgcatctga tcacggccag
     1681 cagcggcaac catgggatcg ccatcgcccg ggccgcggct cgccatggcc tgccggtcac
     1741 gatcctggtc ggcgggtcgg tgtcgccggc caagctcgag cgtctccgag agctggagac
     1801 cgatcgatgc acggtcgaga tcttcggccg cgacacggac gacacggagc ccgaggcccg
     1861 tcgccggcag gcgctcgggg ccggaatcta cgtggcgccg tacaacgatc cgaacgtggt
     1921 cgccggccag ggcacggtgg gagtcgagat cctcgaggag tggccggacg tggacacgat
     1981 cgtggtcccg gtcggcggcg gtgggttgat cgggggcatc ggcctgtggg cgcggacgat
     2041 caacccggcg atccggatcg tgggcgtcca gcccaccgcc tctcccccga tgcaggattt
     2101 cctcgccacc gggcgcgccc gacgtgtccc gatcgggccg acactggccg atggcgtggc
     2161 cggcaacatc gagcgcggct cgatcacctt ccggctggcc aagcgctacg tcagcgaatt
     2221 ggtgctggtc gacgagcgtg cgatcgccga tgcgatgcgc tgggcatggg agggtcccca
     2281 ccatcggctg gagggcagtg ccgcgctcgg tatcgcggct ctgcacaccg gcaagctggg
     2341 tgcgctcgcc gaccaccggg tggccgtcgt cctgagcggt gggaacgtgg acgcatccac
     2401 cttcgacgcg gtggtcgggg ggtagcatgg cgccatgacc gcgaatcgcc agccgccgcc
     2461 gggcgtgatc ctcggcgggg tgctcatcgt catcggtctg tcggtcctcg ccgtccgcta
     2521 cctggacgtc ttcgccggcg agtccatctg gccgctgttc atcatcggtc cgggtctgct
     2581 gatgatcgtc ctgggcctga tcctgcccaa tgttcccatg ctggtcggcg gctgcgtggt
     2641 ggccaccatc ggcggcatcc tcgcctggca gaaccagacc ggcctgtggg caacatgggc
     2701 ctggatctgg gcgctcattc cgatcgcgtc cggggtcggc agcctgctcg gcggcatccg
     2761 caccggcgac cgcgaggcgc gcaactcggg gctgtggcag atcgtcatcg gcgttgccct
     2821 gctcgcggtc ttcttcctgt tcttcgagca gttcatcggg ctctcgggtg gcgagattcc
     2881 ggttcccgag tgggcgatgc ccgcggtgct gatgggactc ggcgttctca tcctgctgcg
     2941 cgacttcttc gaacggccgg accccgaccc gacgacatga ccggcgggcc ggtcgaggtc
     3001 cgaccgctca ccgccaagcg ctggccggac ctcgtggagc tgttcgggcc cagcggcgcg
     3061 ttctcgaact gctggtgcac gtggtggcgg cagaccggtg gcgagttcgg gcgcggcatt
     3121 gcccaacgcg gggcaggcaa caaggctctt ctcggggagc tgacccacgc caaccggcag
     3181 cccggcctgg ttgcctaccg agacgatcgg ccggtgggat ggatcagcgt cgcgccccga
     3241 ccggactacg gccgtgtgat ccgctcgccg agcatcggtc ctggccgccg gagcgccgag
     3301 gcggcggacg agggtgtctg gtcggtcgtg tgcttctgga tgccgcgcaa ggaacgcgga
     3361 aagggaatcg cgatggccct gctccgggca gccgtcgacc acgcccgcgc cggtggcgga
     3421 cgggtcctcg agggctatcc ggtggacacc gcgggggagc gacaccccca gtcgagtgtg
     3481 ttcaccggca ccttggccat gttcaggcgc gtcggcttca cggaggtcga gcgccgggcc
     3541 gagggccggc cgatcgtccg cctcgacctc agttgagtta cgggcgacca tagtcctcgc
     3601 gaccgggccg tcggtcgcct ctatccttag cgggtgcccg ccgacgacac caacggccga
     3661 tccccgctgg acgaggccct cgaagggctg aatgccggct acgtcgccgc ggtctacgag
     3721 cagtaccaat ccgatccggc cagcgtcgag accgagtggc gccggctgtt cgatgccggc
     3781 tacggcggct tcgagccggt tcgaacccca tccccggcgg ctcccgccga tccgtccgat
     3841 ccccccgccg gtgcccggcc gctcaagggt ccagccgcac gcctcgcgtc caacatggca
     3901 gcctcgctgg ccgtccccac cgccacctcg ttccgcgacg tcccggtcgc cgtcctcgag
     3961 gcacggcggc agcagctgaa cgaggcgatt gccccacgca aggtgagctt cacccacctg
     4021 gtcggctacg ccctggcccg ggccgccgga gagcagccgg gcatgacccg cgcgttcacc
     4081 gaggtcaacg gagcggcgca cgtcgtcgag tccggcgcgg tcaacctcgg cctggcggtg
     4141 gacgtcgaac ggcctgacgg cagccggttc ctggtcgtcc cggtcatccg cgccgcagac
     4201 gacgtcgatt tcgccgcttt ccatggccgg tacgaggagc tcgtcgccgg cgcgcgggac
     4261 tcgaccctcg gtcccgacga ctacgccgac gccacgatga ccctcaccaa tcccggcacc
     4321 ctgggcacga cggcctcggt cccgcgcctg atggtcggcc agggctgcat cgtggccacc
     4381 ggtgcgatcc gagacaccgg aagtggccgg gtgatgacca tcacctcgac ctacgaccac
     4441 cgggtgatcc agggcgcgga gtcgggcgca ttccttcggc gcgtggccga cctcctgggc
     4501 ggcgccgacg gcttctacga atcggtgacc gccagcctgg gcgtgacgcc gctccccgat
     4561 ctgccggtgg cggccgcagc cgcgcccgcc ccctcttcgt ctccggtccc ccctaccccg
     4621 gctggcccat cggtcaccgt ctccggcgcg gacctcggtc ggatggcgtc ggcgatggcc
     4681 ctcattgacg cgtaccggtt gtacgggcac ctcgccgccc agctggaccc actcggaacg
     4741 cctccgcccg gcgacccggc cctcgacccg gccttccatg gcctggaccc cgatgccgcg
     4801 ggtcggatca gtgccgacct gctcaaggtc cacgtcggcg gcgacacgct ggccgaggtg
     4861 ctggtgcgcc tgggccggac gtacagcggc acgattgcct acgaggtcga gcacatcgcg
     4921 aaccacgccg agcgcacgtg gctgcgggag gccatcgaat ccggccgatt ccgggtcgcc
     4981 ctcaagcgca aggagcagaa gcggctcctg tcccgcctca ccgcggtcga agggctcgag
     5041 cacttcctgc acaaggcata cctcggccag aagcggttca gcatcgaggg cctggacgcg
     5101 acggtcccga tgctcgacct cgtcctcgag atggccgccg acagcggcac caagcgggcg
     5161 gtgatcggga tggcgcaccg gggccggctc aacgtgctcg cccacatcgt cggcgtgccg
     5221 atgcggtcga tcctggccga gttcgaggcc ggcaagggcg ggcgagcgcc gtccgagggg
     5281 ctcgaaatcg acgacgtgaa gtaccacctc ggagccagcg gcaagaacga gacgcgccgc
     5341 ggcacggtcg acgtcacgct catgcccaac ccgtcgcacc tcgaggccgt gaacccggtg
     5401 gtggagggcc gggcgcgggc cgagcagacc gatcaccgcg gaccgctggt caagatcgat
     5461 acccgacgca ccgttcccat cctccttcac ggcgacgcgg ccttcgcggc gcagggcgtg
     5521 gtggccgaga cattcaacct gtcccggctc gacggctatg ccaacggcgg gaccgtgcac
     5581 ctgatcatga acaaccagat cggcttcacg acatcagtgc gcgaggctcg gagcacgacc
     5641 tgggcgtccg acctggcgaa gggcttcgac gtgccgatca tccacgtcaa tgccgatgat
     5701 ccggaggcat gcctggacgc cgtgcggctg gcgatggcct atcgagccga gttcaacggg
     5761 gacgtggtca tcgacgtcgt gggataccgt cggtacggcc acaacgaggc ggacgagccc
     5821 tcctacaccc agcccggcat gtacgaccgc atcgcccgac agccgtccgt ccgcgcgctc
     5881 tacgccgacc gattggcggc cgatgacgtc gtctccgcga agcagggcgc cgaaatggcc
     5941 gatgaggtca ggcgggacct catcgccgcc caagcctccg tccgcaaggc gcattctgag
     6001 ccgccgcttg accgcggcga ggacgcgctc gccaccgaga gccccgatga gccggccacc
     6061 ggggtcggga tggacgtcct cctggccctc aacgaccagc tccacgccac gcccaagggc
     6121 ttcacggtcc accccaagct cgtccgccag ctcacgcggc gacgggaggc gctggggatc
     6181 gatgagcctg gcatcgaatg ggggcatgcg gagtcgatgg ccttcgccac gctcgtccac
     6241 gacggcgtcc ccattcggct gaccgggcag gacacggttc gcggcacgtt cagccagcgc
     6301 caccaggttc tcgtggacgc ggccaccgac gccacctgga ccccgatgca gcacatcgac
     6361 ggcgccaagg ccagcttcga ggtccacaac tccccgctca gcgagtacgc ctgcctcgcc
     6421 ttcgaatacg gttacgcggt cacggtcccc gacgcgctcg tgttatggga ggcccagttc
     6481 ggcgacttcg tgaacggcgc cgagatcgtg gtcgaccagt tcatcatcgc gggcctggcc
     6541 aagtggcggc agacatcgcg gctcacgctc ctgctccccc atggctacga agggcaggga
     6601 ccggagcatt cgagcgcccg tctcgagcgg ttcctggccc tcggcgcgga gggcaacatc
     6661 cgggtcgcct acccgaccac gtcggcgcag tacttccacc ttctccgctg gcagggcctg
     6721 cacgaggagc tccgaccgct ggtggtcatg accccgaagt cgttgctccg cctgcccgag
     6781 gccgcttccc ggccatccga gctggcaggc ggcagctggg cgccggtcat cgatgacccg
     6841 gagcgtcaga ccgatgcggc cgcgggggcg gtccgtcggg tgatcctgtg cagcggcaag
     6901 atctactacg acctcgccat gtccgaggga cgggccgcgg cggccgacgt cgccctgatc
     6961 cgggttgagc agctctaccc gttccccacc gagaccctgg ccacggtgct cagccgctac
     7021 cgacggatgc gccgggtgag ctgggtgcag gaggaaccgc ggaacatggg agcccgcaag
     7081 ttcgtgctgc ccaagatccg ttcgatcgtc gcggcgcgga tcccgctcgg cgacgtctca
     7141 cgcccggaac gctcgcgccc ggcggagggc tacccggcgg cccacgccgc ggagcaggcc
     7201 cggatcgtgc gcgacgcctt cgccgactag gcgagagccc acgcgcggat gaccgatctg
     7261 accacggccc gttccccctc ggctctgctc gagcggtacg cggacctgct cgcccgcccg
     7321 ggcgagtcat tcccgatctc cctgggcgag ggcgcgacgc cgctgatccg cgctcgccgg
     7381 ctgggcgccg ccgtcggcgt tgaccggctc ttcctcaagt tcgagggcac gaatcccacc
     7441 ggctcattca aggaccgggg catggtcctg gccgtgaacc gggccgtggc agcagaggca
     7501 cgaatggtcg tctgcgcgtc gaccggcaac acgagcgctt cggccgccgc ctatgcggcc
     7561 gcagccggac tccggtgcgg ggtcgtcctg cccgcgggca aggttgcgcg cggcaagctg
     7621 gcccaggccc tcgcggcggg ggcgcggctg gtgctgaccg atggcaactt cgacgccgcc
     7681 ctcgaggcgg tgcgcgagct tggacagagt ggcgaggcgg tggtcgtgaa ctcgatcaac
     7741 cctgaccggc tggagggcca gcagacggtg gcatgggaga tctgcgatac gctcggcgcg
     7801 gcccccgacg cggtcgcgct gccggtgggc aacgccggca acatcaccgc cgcctggcgc
     7861 ggcttcgtgg cgtacgccga ggccggtcga gcggacgcgc gcccgcgaat gctgggcttc
     7921 caggccgccg gcgcggcccc catcgtcaac ggagcggccg tcgcggaccc ggaaacggtg
     7981 gccactgcga tccggatcgg gaacccggcc tcgtgggcgg gggcgacggt tgcccgggac
     8041 gagtccggcg ggctgatcga ggcggtcacg gacgagcaga tcctcgccgc ccaggccgac
     8101 atcgcccgac tggagggcgt gttctgcgag ccggcgtcag cggcgggtgt ggcgggagtg
     8161 cggcagctgg ccgccagtgg ccgcctggag cgatcggcga ccgtggtctg catcctcacc
     8221 ggccacggcc tgaaggaccc ggacacggcc cccggccacg cgggagacct ggcaccggtg
     8281 cccgcgacgg cggacgccat tcgcgccgca ctcgatggct gacctcgccc gcccacgctg
     8341 ccagtgggta gccgatgccg gcgacgcgat gctcgcgtac cacgacgacg agtggggtgt
     8401 cccgatccac gatgaccggg cactgttcga gctcctgaca ctcgagggtg cgcaggccgg
     8461 tctcagctgg tccacgatcc tgaaccgccg ggacggctac cggcgcgcgt tcgcgggctt
     8521 cgacatcgtg gcggtcgcgg ccttcggatc cgacgaccag gcccggctca aggccgacga
     8581 tgggatcatc cgaaacgcgg cgaagatccg ctccgcgatt gggaacgctc ggctcaccct
     8641 ggtgctcggc aacctggatg cgcacctgtg gtcgttcgtg ggcggacgcc cgatccagaa
     8701 cgcctggcgc ggccatgatg tcccggccga gaccgccgag tcgaaggcga tgtcccgtga
     8761 cctgaagcgg cgtgggttct cgttcgttgg tccgaccatc tgctacgcct tcatgcagag
     8821 cgcaggactc gtgaacgatc acgcgctcct ctgcttccgc catgacgagg tggcacggct
     8881 ggcctgaccg atacactcgg cgacgtggcc acccccatcg catcggtcga tgttccagcc
     8941 agcaccgcga acctgggcag cgggttcgac tgcttcgcgg cggcgctgag cctgaagctg
     9001 cgggcggaac tgcatcacgg caacctgccc gggatcgtgc tgaacgccca cggcgagggc
     9061 gcgatcgagg ccgagagcgc cggttcggac agcatggtcg tccgcgcgtt tcgcgaaggg
     9121 ctcagggcgg ccggggggcc gccgggcggg agcgcctcgt ggcggatcga ggtctcatcc
     9181 atgatccccg ccgcgcgcgg gctgggcagc agcgcggccg cgatcctggc cggaatcctg
     9241 ctcggtgctg ccgcgggtag gctgcccacg aacgccgacc agctccttgc cctcgcggcg
     9301 cggctcgagg gccatccgga caacgtctcg gccgcgctct acggcggctt caccctgtcg
     9361 atcatggagg ccggcgacgt caccgtgcgc cgcttccggg tccccgaggc gtggatcccg
     9421 gtcctgttca tcgcccaaac cgagagccgg accgaggaca tgcgcgccgc gctgccgggc
     9481 cgggtcccgc tgcccgacgc cgccaaccag gcggcacgtg ccgcgctgct cgccaccgcg
     9541 gtgctcacct cggacgcgtc gctcctgcgg cgggccatgg acgaccgcct gcaccagccc
     9601 taccgcctcc ccctgctccc cggttccgcc cgtctgatcg agaccgcgta ccagatcggg
     9661 gcagccggag cgtgcctgtc aggggccgga ccgtcggtgc tggccatctg cgactcgcca
     9721 ctcaccgccc attcggtgga gaaggcgtgg aacggatcgg gccaggacgg gatggccacc
     9781 cggctcaggt tcgacacggc cggcgccaag ctgaacgcgg cggaagcccg ggagtgagcg
     9841 acctcggatg agcctcgtct ccctgtccgg ggtctcgaag agccacggcg cccagcacct
     9901 gttctccgac gtcgcgctcc aggtcagcgc gggccggcgg gtggcgatcg tgggcgccaa
     9961 cgggaccggc aagaccacgt tgctcgagat catcaccggc gagcaggacg cggacaccgg
    10021 ggtggtggcc cgaggcaagg acgtggtcat cggctacctc cgccaggaag tggccgagtc
    10081 gcgcggtcac tcggtgctgg ccgaggtgct ggccggcgcc gggacggtga ccggcatcgg
    10141 ccgccggatg cagcacatcg agcaggagat ggccgaggcc atcgccgagg acgagctggc
    10201 cgagctcatg gacgagtacg gcaggctcca gcaccggttc gagcagctgg gcggctggtc
    10261 gatcgaatcg gaggcccggc gcatcctggc cggcctgggc ttcgccgaag ccgatgccga
    10321 gcgtgacatc ggcgagttct ccggcggctg gatgatgcgg gtcgcgctgg cccggctgct
    10381 gct
//
LOCUS       DASYGU010000203         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_393678, whole genome shotgun sequence.
ACCESSION   DASYGU010000203 DASYGU010000000
VERSION     DASYGU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393678"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VGO32_05670"
     CDS             <1..581
                     /locus_tag="VGO32_05670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7200274.1"
                     /translation="VTWDDAYRGQPPWEIGRAQRVFIDLEEAGAIEGSVLDVGCGTGE
                     NALHLAERGHDVWGIDVSSVAVERAMVKSHARRQPVVFLVADALEPEAVGRTFDTLLD
                     CGCFHTLSDDERVRYRASMWALGAPGSRLHVMCFSEREPPGWGPRRVTEAELRSTFED
                     GWSVDGITAVRFETRLGPNGARAWLASFTRTT"
     gene            641..>1707
                     /locus_tag="VGO32_05675"
     CDS             641..>1707
                     /locus_tag="VGO32_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEV7200275.1"
                     /translation="MQLIDGSPVYSATDLVGYLECEHLTALERAVLAGLAERPHVTDP
                     ELDVLRRRGEQHERRYLAELTGEGRSVVEITLDGSIEDRGEQLRAAAAETERAMAAGS
                     DVIYQATFFDGTWRGHADFLLRVNDPDRPSQWGPYHYEVADTKLARHVKASAVLQICV
                     YVDMLTAVQGIEPAALHVVLGGSAGGLRTLRVADYMAYYRAARARFDAAVGPEAPAAV
                     YPPASYPEPVDHCDVCRWRVVCAQRRRDDDHLSLVAGISRRQRRALVDRGVATLEALG
                     ELPLPVTPPLERTSASALERVREQARIQLEGRRARDHRYELFLPAPGEPVPVDLGLAA
                     LPEPSPGDLFFDIEGDPYAGD"
BASE COUNT          233 a          612 c          599 g          263 t
ORIGIN      
        1 ccgtgacctg ggacgatgcg tatcgcggcc agcccccgtg ggagatcgga cgtgcccaac
       61 gggtgttcat cgacctcgag gaggccggcg cgatcgaggg cagtgtcctg gacgtgggct
      121 gcggcaccgg cgagaacgcc ctccacctcg ccgagcgcgg acacgacgtg tggggcatcg
      181 acgtctcctc ggtggccgtc gagcgggcga tggtcaaatc ccatgcccgt cgccagccgg
      241 tcgtgttcct ggtcgccgac gccctcgagc cggaagccgt tggtcggacc ttcgacacgc
      301 tcctcgactg cggctgcttc catacgcttt ccgatgacga acgggtccgc taccgggcca
      361 gcatgtgggc cctcggtgcc cccggcagcc gcctccacgt catgtgcttc tccgaacgcg
      421 agccacccgg atggggtccg cggcgggtga ccgaggccga gctgcgctcg acgttcgagg
      481 acggctggtc ggtcgacggg atcacggcgg ttcggttcga gacgcggctg gggccaaacg
      541 gggcccgcgc ctggctggcg agcttcaccc ggaccacctg accgatcgat ctcgcagcgc
      601 gacaggccag ctgtcgcacc ggtcagccca caatgcgcct gtgcagctga tcgacggatc
      661 gcccgtctac tcggcgaccg acctggtcgg ctatctcgag tgcgagcacc tgaccgcgct
      721 cgaacgggcc gttctggcgg gcctcgcgga gcgcccgcat gtgaccgatc cggagctgga
      781 cgtcctgcgc cggcgcggcg agcagcacga gcgtcgctac ctggcggagc tcaccggcga
      841 gggccgatcg gtggtcgaga tcacgctcga cggatcgatc gaggatcgcg gcgagcagct
      901 gcgggcggcc gcggccgaga cggagcgggc gatggctgct ggatcggacg tcatctacca
      961 ggccacgttc ttcgatggca cctggcgcgg ccacgcggac ttcctgcttc gggtgaacga
     1021 cccggatcgg ccgagccaat ggggcccata tcactacgag gtcgcggaca ccaagctcgc
     1081 tcgccacgtc aaagcgtccg ccgtgctcca gatctgcgta tacgtggaca tgctgaccgc
     1141 cgtccagggc atcgagcccg ccgcgctcca cgtcgtcctg ggcggttccg ccggtggcct
     1201 gcggacgctt cgggtggccg actacatggc ctactaccgg gccgcccgcg cccgcttcga
     1261 cgccgcggtc gggccagagg ctccggctgc ggtctacccg cccgcctcgt atcccgagcc
     1321 ggtcgaccat tgcgacgtct gccggtggcg ggtggtctgt gcacagcgtc gtcgggatga
     1381 cgaccacctg agcctggtcg ccggcatctc ccgccgtcag cgacgcgccc tcgttgaccg
     1441 cggcgtcgcg accctcgaag cgctcggtga gcttccgctc ccggtcacgc cgccgctcga
     1501 gcggacgagc gcgagcgccc tggagcgggt ccgggagcag gcacgcatcc agctggaagg
     1561 gcgccgggcg cgggatcacc gctacgagct gttcctcccc gcgccgggtg agccggttcc
     1621 ggtcgatctc ggtctcgccg ccctgcctga gccctcgccg ggcgacctgt tcttcgacat
     1681 cgagggcgac ccgtatgccg gtgacga
//
LOCUS       DASYGU010000204         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1164702, whole genome shotgun sequence.
ACCESSION   DASYGU010000204 DASYGU010000000
VERSION     DASYGU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164702"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGO32_05680"
     CDS             complement(<1..327)
                     /locus_tag="VGO32_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200276.1"
                     /translation="MTTQRAMLVPRWLERAGALSWRIAVLIGLGAVLTWLAFTLGTVT
                     ASVIVALIVAATFAPLGQGLRRRGWSGTASAAAVTGAAVLVGGLLLLLLALAFAPYIP
                     TLVSAFQ"
     gene            396..1022
                     /locus_tag="VGO32_05685"
     CDS             396..1022
                     /locus_tag="VGO32_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200277.1"
                     /translation="MVSDASTHDSFGRWAGAAAIAASIGGVIYSAAFLGGVVLGFNPE
                     LGILVASAALMVGGLLSTVVLVAIYERVGGGSLALVGLACGLLGALGAIVHGGYDLAI
                     ALNAPTSDPITDAGLPSPVDPRGLLTFGMAGLGVLILSAIARANGTFSMPVATIGIVL
                     GILLILVYLGRLIILTPTSPLVAVPAGLAGVIVGPFFYFAVGLELRRR"
     gene            1068..3146
                     /gene="fusA"
                     /locus_tag="VGO32_05690"
     CDS             1068..3146
                     /gene="fusA"
                     /locus_tag="VGO32_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV7200278.1"
                     /translation="MKPTTPDRIRNVALIGHGGAGKTSLAEAILFDAGGIPRLGSVEA
                     GTAALDWEPDEQRRHQSINLGIGTVDTEGARITLVDTPGYADFQADVVQALAAVDAAI
                     VVVDAAAGVEVGTDATWRLADERGLPRMVFVNQMDRENANYDATLLQLKAAFGPKIAP
                     VYLPIGSAESFRGYIDVVEQHASIYEGGEAKEVPIPDEMRAAEESRRTALIEAAAEAS
                     DELMMKYLEGEEISDEEIETALHAGTRDGSVVPVFVGSAVKNIGVRELIRMIARHVPS
                     PAEVGSRKTADGATISPDPSGPFVAQVFKVTADPFVGRLTYFRVVSGMLKAQGHIHNA
                     NRREDERVGNILGLQGKEQVNLPQVGPGDIVAVAKLASTHTGDTLVAERAGAVQLPPL
                     VFPEPTLQVAVEPESKADLDKLGQALNRIVEEEPCMRVRREEATSQTIVTAMGDAHVD
                     VIVERLKRKFGAAVKTGTPRVPYRETIRRPTKIDNRFKRQTGGHGQFGHVVIEFEPNE
                     TGEGFVFGDRIVGGAVPKQYIPAVEKGLRESMAEGVLAGYPVVDLKATLVDGSYHTVD
                     SSEMAFKIAASQALKRAFTEADPALLEPILEVEVTVPDEYMGDVMGQITAKRGHVLGM
                     NSVDGTQYLRAQVPQAEMFHYATELRSITQGRGRFSQSLDHYAEVPHTIAEKVIAEHQ
                     AKAPTVERGH"
     gene            complement(3147..>3986)
                     /gene="tyrS"
                     /locus_tag="VGO32_05695"
     CDS             complement(3147..>3986)
                     /gene="tyrS"
                     /locus_tag="VGO32_05695"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV7200279.1"
                     /translation="EWFDEFRLREVFALMSRFTLARMIERDTFAKRLAEGAPVSMHET
                     MYPLLQAYDSVAIEADVELGGTDQTFNLLVGRDVQRDYGQAPQQIMTSELLVGIDGVQ
                     KMSKSLGNAIGLTDAPYDLYARTMSIPDTLLPNWAGLVTRWADAEVDAFVADLQADRI
                     HPKAAKQRLAREIVARWHTDADADTAEQRWEREVGGGEGMGISELEEMDVSVALAGIS
                     LPDLLVAAGLASSRSVGRRLVRQAGVRIDGEVQTDENRMFAAGLSHEVRVGKRSAARV
                     RLT"
BASE COUNT          633 a         1422 c         1342 g          589 t
ORIGIN      
        1 ctggaaggca gacacgagag tcggaatgta cggcgcgaac gcgagcgcca ggaggaggag
       61 cagcaggccc ccgacgagga ccgcggcccc cgtcaccgcg gccgcactcg cggtccctga
      121 ccagccacgg cgacgcagac cctgaccgag cggggcaaac gtggcggcca cgatcagggc
      181 aacgatgacc gaggcggtga ccgtccccag cgtgaaggcc agccaggtga gcacggctcc
      241 caacccgatc aggaccgcga tccgccagct gagcgcaccg gcccgttcga gccagcgcgg
      301 caccagcatg gccctctggg tggtcaagtc gggccggatc ggtgcatgtc ggatcaggat
      361 agcgggcagc gcggtgccaa atgtatgctc cggccgtggt ttcggacgcc tcgacacacg
      421 actcgttcgg acgctgggcg ggcgccgcgg ccatcgcggc cagcatcggc ggggtcatct
      481 actcggccgc gttcctgggc ggggtcgtgc tcggcttcaa tcccgagctc gggatcctcg
      541 tcgcctcagc cgccctcatg gtcggcggcc tgctcagcac ggtggtgctg gtggcgatct
      601 acgagcgggt gggcggcggc tcgctggcgc ttgtcggcct cgcgtgcggg ctcctcggcg
      661 cgctcggggc gatcgttcac ggcggctacg acctggccat cgccctcaac gcgccgacct
      721 ccgacccgat caccgacgcc ggcctgccga gcccggtcga tcctcgcggc ctgctgacct
      781 tcggcatggc gggcctcggg gtcctgatcc tctcggccat tgcccgggcc aacggcacct
      841 tctcgatgcc ggtcgcgacc atcggcatcg tgctcggcat ccttctgatc ctggtgtacc
      901 tcggccggct catcatcctg accccgacca gcccgctggt cgcggtcccg gccggcctgg
      961 ccggggtgat cgtcggaccc ttcttctact tcgccgtcgg tctggagctc cgtcgccgct
     1021 gagcgtcaaa cgctccctat aatggccgcc ctgcccgagg atctgccttg aagccgacca
     1081 cgcccgaccg gatccgcaac gtcgccctca tcgggcacgg tggcgccggc aagaccagcc
     1141 tcgccgaggc catcctgttc gacgccggcg gcatcccgag gctcggatcg gtcgaggccg
     1201 ggacggccgc cctggactgg gagccggacg agcagcgtcg acaccagtcc atcaacctgg
     1261 ggatcggcac ggtcgacacc gagggcgcgc ggatcaccct cgtcgacacc ccgggctacg
     1321 ccgacttcca ggccgacgtc gtccaggccc tggccgcggt ggatgccgcg atcgtggtcg
     1381 tggatgcggc ggccggggtc gaagtcggga ccgatgcgac ctggcggctg gccgatgagc
     1441 gcgggttgcc ccggatggtc ttcgtcaacc agatggaccg cgagaacgcg aactacgacg
     1501 cgaccctgct ccagctgaag gcggcgttcg gacccaagat cgcgccggtc tacctcccca
     1561 tcggctcggc ggagtccttc cgtggctaca tcgacgtcgt cgagcagcac gcctccatct
     1621 atgaaggtgg cgaggcgaag gaggtgccca tcccggacga gatgcgcgcc gccgaggagt
     1681 cgcgccggac cgcgctcatc gaggccgcgg ccgaggcatc ggacgagctg atgatgaagt
     1741 acctcgaggg cgaggagatc tccgacgagg agatcgagac cgcgctccac gcagggacgc
     1801 gggatggatc ggtggtgccc gtgttcgtcg gatccgcggt caagaacatc ggcgtccgcg
     1861 agctcattcg gatgatcgcc cgccacgtcc catcgccggc cgaggtggga tcgcggaaga
     1921 ccgccgacgg cgcgaccatc tcgcccgacc cgtcgggccc gttcgtggcc caggtgttca
     1981 aggtcaccgc cgatccgttc gtcggccggc tcacctactt ccgggtcgtg tccggcatgc
     2041 tcaaggccca gggccatatc cacaacgcca accggcgcga ggacgagcgg gtgggcaaca
     2101 tcctcggtct gcagggcaag gagcaggtca acctgccgca ggtcggcccg ggcgatattg
     2161 tggccgtggc caagctggcc tccacccaca ccggcgacac cctcgtcgct gaacgggctg
     2221 gcgccgttca gctgccgccg ctcgtgttcc ccgagcccac actccaggtg gcggtcgagc
     2281 cagagtccaa ggccgacctc gacaagctcg ggcaggccct gaaccggatc gtcgaggagg
     2341 agccgtgcat gcgggtccgc cgcgaagagg cgacctccca gacgatcgtg accgccatgg
     2401 gcgacgcgca cgtggacgtc atcgtcgagc ggctgaagcg caagttcggc gcggcggtga
     2461 agaccgggac cccacgagtc ccctaccgcg agaccatccg acgaccgacc aagatcgaca
     2521 accggttcaa gcggcagacg ggtggccacg gccagttcgg gcacgtggtc atcgagttcg
     2581 agccgaacga gaccggcgag ggcttcgtct tcggcgaccg gatcgtgggt ggggccgtgc
     2641 ccaagcagta catccccgcc gtcgagaagg gcctccgcga gtccatggcc gagggcgtgc
     2701 tggccggcta cccggtcgtc gacctcaagg cgaccctggt cgatggctcg taccacacgg
     2761 ttgattcgtc cgagatggcc ttcaagatcg cggcctcaca ggccctcaag agggccttta
     2821 ccgaggccga tccggccctg ctggaaccga tcctcgaagt cgaggtcacc gtcccggacg
     2881 agtacatggg cgacgtgatg ggccagatca ccgccaagcg cggccacgtg ctggggatga
     2941 actcggttga cggcacccag tacctgcgcg cccaggtccc acaggccgag atgttccact
     3001 acgccaccga gctgcggtcc atcacgcagg gccgtggccg cttctcgcag tcactggacc
     3061 actacgccga ggtcccgcac acgatcgccg agaaggtcat cgccgagcac caggccaagg
     3121 ccccgaccgt ggagcgcggc cactagtcag gtcaggcgga cgcgcgccgc cgaacgcttc
     3181 ccgacccgca cctcgtggct caatcccgcc gcgaacatcc ggttctcgtc ggtctgaact
     3241 tcaccgtcaa tccggacccc cgcctggcga acgaggcgtc ggccaacgct ccgtgaggac
     3301 gccagccccg cggcgacgag caggtcaggc aggctgatcc cggccagcgc gaccgagacg
     3361 tccatctcct cgagctccga gatgcccatg ccctcgcctc cgccgacctc ccgttcccag
     3421 cgctgctccg cggtgtcggc gtcggcatcg gtgtgccaac gggcgacgat ctctcgcgcc
     3481 agtcgctgct tggccgcctt ggggtggatc cggtcggcct ggaggtccgc gacgaaggcg
     3541 tccacctcgg catcggccca gcgggtcacg agcccggccc agttcggcag cagcgtgtct
     3601 gggatgctca tcgtgcgggc gtacaagtcg tagggcgcat cggtcaggcc aatcgcgttg
     3661 cccagcgact tgctcatctt ctgaaccccg tcgatcccga ccagcagctc cgaggtcatg
     3721 atctgctgcg gcgcctggcc gtagtcgcgc tgcacgtcgc ggccgacgag caggttgaac
     3781 gtctggtcgg tgccgcccag ctcgacatcg gcttcgatcg cgaccgagtc gtacgcctgg
     3841 agcaacgggt acatcgtctc gtgcatcgag accggggcgc cctcggccag gcgcttggcg
     3901 aacgtgtcgc gctcgatcat gcgcgccagc gtgaaccggc tcatgagcgc gaagacctcg
     3961 cgcagccgga actcgtcgaa ccactc
//
LOCUS       DASYGU010000205         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_342302, whole genome shotgun sequence.
ACCESSION   DASYGU010000205 DASYGU010000000
VERSION     DASYGU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342302"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..521
                     /gene="rplN"
                     /locus_tag="VGO32_05700"
     CDS             153..521
                     /gene="rplN"
                     /locus_tag="VGO32_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV7200280.1"
                     /translation="MIRRQTRLRVADNTGARELMCITVVGRSTSETASVGDVIVASVK
                     QALPNSGVKKGEVVRAVIVRTTKGYGRPDGSHIRFDENAAVLITPQGNPRGTRIFGPV
                     ARELRERNYMKIISLAPEVL"
     gene            521..856
                     /gene="rplX"
                     /locus_tag="VGO32_05705"
     CDS             521..856
                     /gene="rplX"
                     /locus_tag="VGO32_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV7200281.1"
                     /translation="MAVRKGDTVMILAGKDRGKRGKVERIERTRRGLAAIIPGINMAK
                     RHQRSRGGRQQTAGIIDMPIPVQISNVMVVCPNCDKPTRIGHARLDDQHKTRVRVCKH
                     CGEQLEVGK"
     gene            994..>1545
                     /gene="rplE"
                     /locus_tag="VGO32_05710"
     CDS             994..>1545
                     /gene="rplE"
                     /locus_tag="VGO32_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV7200282.1"
                     /translation="MSPARRRSAGRSEPSGLRLRYRDEAVPVLLREFRYANAMEVPRL
                     EKVVINIGLGEAIQNAKAIDAAVGDLMIISGQKPIVTRATRSIAQFRLRTGMPVGAKV
                     TLRGQRMYDFLERTMRLALPRIRDFRGVSPRSFDGRGNFSLGFREQLMYPEIDYDKID
                     RLRGFEISIVTTARTDEEGRRLLA"
BASE COUNT          266 a          556 c          522 g          201 t
ORIGIN      
        1 ctgtcgcgtg acaagcgctg gcgcgtcgtc gagatcctgg gccatggtcc ctcggagacc
       61 gcgacgggac cgatggccga cgaggccatt ctgaatcccg agccggtggc ggatgccgac
      121 gccgagcccg ccgcggtgga gggagagtcc gcgtgatccg ccgccagacc cggctgcggg
      181 tcgccgacaa caccggcgcc cgtgagctca tgtgcatcac ggtggtgggc cgctccacga
      241 gcgagacggc ctcggtcggt gacgtcatcg tggccagcgt caagcaggcc ctgcccaact
      301 ccggggtcaa gaagggcgag gtcgtccgcg cggtcatcgt ccgcacgacc aagggctacg
      361 gccgtcccga cggaagccac attcgcttcg acgagaacgc tgcggtcctc atcaccccac
      421 agggcaaccc gcgcggcacc cgcatcttcg gcccggtcgc ccgcgagctc cgggagcgca
      481 actacatgaa gatcatctcg ctcgcgccgg aggtgctgtg atggcagtcc gcaagggcga
      541 caccgtgatg atcctggccg gcaaggaccg cgggaagcgg ggcaaggtcg agcgcatcga
      601 gcggacgcgc cgtggcctgg ccgcgatcat ccccggcatc aacatggcca agcgccacca
      661 gcgctcgcgc ggtggccgcc agcagacggc gggcatcatc gacatgccca ttcccgtcca
      721 gatcagcaac gtgatggtgg tctgccccaa ctgcgacaag ccgacccgga tcggtcacgc
      781 gcggctggac gaccagcaca agacccgggt ccgcgtgtgc aagcactgcg gcgaacaact
      841 ggaggtgggc aagtgaccga cgagccgacc aaggcccccc gtaaagccgc cgccaaggcg
      901 ggcgcatcgg ccaagccggc gacccgtgcc aagccgcccg cgaagaggtc agccggccgg
      961 gcgaagaccg agtccgccgc gccggcggag accttgtctc ccgcgcgtcg acggtcggcc
     1021 gggcgctccg agcccagcgg ccttcggttg cgctaccgcg acgaggcggt gcccgtgctg
     1081 ctccgggagt tccgctacgc gaacgcgatg gaggtcccgc gcctcgagaa ggtggtcatc
     1141 aacatcggcc taggcgaggc catccagaac gccaaggcga tcgacgccgc ggtgggcgac
     1201 ctcatgatca tcagcggcca gaagccgatc gtgacccggg ccacccgctc catcgcccag
     1261 ttccggctcc ggaccggcat gccggtgggt gccaaggtga ctcttcgggg ccagcgcatg
     1321 tacgacttcc tggagcgcac gatgcgcctg gccctgcccc ggatccgcga cttccgtggg
     1381 gtctcgccgc gctcgttcga cggccgaggc aacttcagcc tcggattccg cgagcagttg
     1441 atgtaccccg agatcgacta cgacaagatc gaccgcctcc gcggtttcga gatcagcatc
     1501 gtcaccaccg cccgcacgga cgaggaaggc cgccgcctcc tcgcc
//
LOCUS       DASYGU010000206         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1421886, whole genome shotgun sequence.
ACCESSION   DASYGU010000206 DASYGU010000000
VERSION     DASYGU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421886"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGO32_05715"
     CDS             <1..204
                     /locus_tag="VGO32_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200283.1"
                     /translation="LLNLIPIPPLDGSAVLLAVVPPRWEYVIRQYQGFGVALLVILLL
                     VPNSPLSAMLGLAEPWAAILCGI"
     gene            216..710
                     /locus_tag="VGO32_05720"
     CDS             216..710
                     /locus_tag="VGO32_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200284.1"
                     /translation="MRPVYRVRQFGTHLFARVSADERADAHEVLPPAAAVIFDRMPVA
                     DQRHAIDVLARLRASGQTDGDLLAAALLHDAGKGTRVRLWHRVAAVLVGAVAPSRMER
                     LGSSDPRSWRYPFHLQRHHEALSAQAALAAGCGPRVAAFIVGSAEGTDAQLMAALAAA
                     DAAS"
     gene            729..1505
                     /locus_tag="VGO32_05725"
     CDS             729..1505
                     /locus_tag="VGO32_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV7200285.1"
                     /translation="MSHGIPIEPDPRVAVTVGPPPEFQVDLGVFEGPLPLLLHLIESA
                     ELDILTVPLARVADAYVSYLAIHPVDAANLAAFVATAAQLILIKSRSLLPAEPAIDPL
                     TGAEDIDEEELRRRLLAYRAIRDASRELAARDQVSPMWHREPRAADLPEAPLAALDHG
                     ILAAALAGLAAVAEPEPEPPEIVPREVTVGQQIRVLRAALGDRGSLVLQTVLAVSTSR
                     TERVVTLLAALELVRRRELRATQKTLFGPIVLEPAPGTRS"
BASE COUNT          195 a          636 c          551 g          247 t
ORIGIN      
        1 ctgctcaacc tcatccccat cccgccgctc gatgggtcgg cggtgctgct cgccgtcgtg
       61 ccgccacgct gggagtacgt gatccgtcag taccagggat tcggggtcgc cctgctcgtc
      121 atcctgctgc tcgtgcccaa cagcccgctg tcggccatgc tcggcctcgc cgagccttgg
      181 gccgcgatcc tgtgcgggat ctgacccgag atccgatgcg ccccgtctac cgcgtgcgcc
      241 agttcgggac ccacctgttc gcccgcgtat cggcggacga gcgcgcggac gcgcacgagg
      301 tcctgccccc cgccgccgcg gtcatcttcg accggatgcc ggtggccgac cagcgccatg
      361 cgatcgacgt cctggcccgg ctccgggcgt cgggacagac cgatggcgac ctgctggccg
      421 ccgccctgct ccacgatgcg ggcaagggca cgcgggtccg cctgtggcac cgcgtggccg
      481 cggtcctggt cggggcggtc gctccgtccc ggatggaacg cctgggctcc agcgacccga
      541 ggtcgtggcg atacccgttc cacctccaac gacaccacga ggcgctctcc gcccaggcgg
      601 cgctggccgc cggctgtggg ccgcgggtcg ccgcgttcat cgtcggcagc gcggagggga
      661 ccgatgcgca gttgatggcc gcattggcag ccgccgacgc ggcatcctga tcgcatggtg
      721 ctgacccgat gagccacggc atcccgatcg agcccgatcc gcgggtcgcg gtcacggtcg
      781 ggccgccgcc cgagttccag gtcgacctcg gtgtgttcga gggcccgctc ccgctcctgc
      841 tccacctcat cgagtccgcc gagctcgaca tcctgaccgt acccctggcc cgcgtggcgg
      901 atgcctacgt cagctacctc gccattcacc cggtggatgc cgccaacctg gcggcgttcg
      961 tcgcgaccgc cgcccagctc atcctcatca agtccaggag cctgctcccc gccgagccgg
     1021 ccatcgaccc gctgaccggc gccgaggaca tcgacgagga ggagcttcgc cgtcggctgc
     1081 tggcctaccg ggccattcgc gatgcgagcc gcgagctggc ggcgcgcgac caggtctccc
     1141 ctatgtggca ccgtgagccg cgggccgcgg acctgccgga ggcgccgctc gcggcgctcg
     1201 atcacggcat cctggccgcc gccctcgccg gcctcgccgc ggtggcggag ccagagcccg
     1261 aaccgcccga gatcgtcccg cgcgaagtca cggttggcca gcagatccgg gtcctgcgcg
     1321 ccgctctcgg cgatcgtggc tcgctcgtgc tccagaccgt cctggccgtc agcacgagcc
     1381 ggaccgagcg ggtggtcact ctccttgccg cgctcgagct cgtccggcgc cgtgagctgc
     1441 gtgccaccca gaagacgctg ttcgggccga tcgtgctcga gcccgctccg gggacccgat
     1501 cgtgaccggc gccgcaccca tcggtccggg aggtgagctg gccgcccagc tggaggccct
     1561 gctgttcgtg gccgatcgac cgctgaccac cgccgagctg gccgagctgg ctaccgtcac
     1621 gccggccgc
//
LOCUS       DASYGU010000207         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1473566, whole genome shotgun sequence.
ACCESSION   DASYGU010000207 DASYGU010000000
VERSION     DASYGU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473566"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VGO32_05730"
     CDS             complement(<1..1907)
                     /locus_tag="VGO32_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7200286.1"
                     /translation="MAAPAAIAGAEQPGPSAERRLVSVLFADLVGSTTLAEDRDPEET
                     RNLLTRYFDTASEVIARYGGTVEKFIGDAVMAVWGVPVAHEDDAERAVRAALDLVSSV
                     GTIIDQGQPLQVRAAVLTGEAAATIGAAGQGIVAGDLVNTASRLQSLAPPGTVLVGES
                     THRATAEAIAYEEAGEQLLKGKSSPIPAWRAVSVLGMRGGAGRRSALEAPFVGRDEEL
                     RLVKDLFHATIREQKPRLVTIVGQAGIGKSRLGWEFEKYIDGVTIDAYWHSGRSPSYG
                     EGISFWALAEMVRERAGIAESDSVEEAATKLSACLDEWLTDAEERRWVQPRLAGLLGL
                     DEMPTGQRDELFAAWRTFFQRIAERDPVILVFKDLHWADAGLLEFIEHLLSWSKTHPI
                     YVLAMTRPDLFERHPAWGSGVRNATTVSLEPLTEAAMADLLRGLAPGLPEDAVTAIVA
                     RAEGVPLYAVETVRMLIDRGQLEASDGGYALTAPIDRLAVPETLHALVAARIDANAPE
                     DRSLLADGAVLGQSFTAAAMAGMGGRDLEMVEPGLDRLVQRELLIRDDDPRSPERGQY
                     RFVQAVVREVAYETLSKADRRNRHLAAARYYEALGDDELAGVLASHYLEALRSTPAGP
                     EADALAAQARIA"
     gene            complement(2084..>5116)
                     /locus_tag="VGO32_05735"
     CDS             complement(2084..>5116)
                     /locus_tag="VGO32_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7200287.1"
                     /translation="AVLTGEAAASIGAVGQGIVAGDLVNTASRLQGMAPAGTVLVGES
                     TYRATRDAIAFEDVGDQVLKGKSSPVPAWRAVQVLGMRGGARRRSALEPPFVGRDEEL
                     RLVKELFHATIRESKPRLVTIIGQAGIGKSRLGWEFEKYIDGVNQDFYWHAGRSPSYG
                     EGISYWALAEMVRERAGIAETDDVPTARTKLTACLDQWLIDSEERRWVEPRVAGLLGL
                     EELPATQREDLFAAWRTFFERIADHDPVILVFKDLHWADAGLLEFIEHVLAWSKNHPI
                     YVVAMTRPDLLERHPNWSSGVRNATTVVLEPLPEGPMRELLHGLVPGMPDAAVDAVVQ
                     RAEGVPLYAVETVRMLLDRGQLQSTDEGFVLAGPIDRLAVPETLHALVAARIDANTPE
                     DRAVLADAAVLGQSFTMDALSGVTGQDESTLTPGLDRLVRRELLIRDDDPRSPERGQH
                     RFVQAVVREVAYETMAKADRRAKHLAAARYFEALGDDEISGVLASHYLEALRATNPGP
                     EADAVAIQARIALRAAAERAEALHSYAAAYHHAEDAIAITTDPLEQAALHELAARSGS
                     LLALPEAIEHAQTAAELASAGDDRATANRALALTARIQTDYGKSDGIGTLEPVAATMA
                     EDEPEAARVFAELARLYMLTDQNDEAIETAERALRAAGPSRDTEVVVEALTTQATALW
                     YKRPDEGDAVFRGAIQLADREGHVTSALRARNNWSSNLLFDRPYHEAAEVVSDGIDLA
                     RRFGLASWLGVFLTSRAEIRMYTGEWAGAALDVAEVDERASPPDVAGWVTMIRGLLAA
                     VVGDADAYRAAADDSARVLAGEDMLAPQLGGIRIVEAQALLALGDPRAALESVAGLAG
                     GGNDLYLAMTRSLATALLGDVGAHAAIPVEFDFPEQATWTRGLRQKMAASDAVLAARW
                     EEARMAYADTVATYQGLESNLWTSWIGLEFDAFLGAAFEDARRAGEEAAEFFASRGAE
                     GVVDRYRQSFQGTPAPASGARAASAAFARDSVSEVEAR"
BASE COUNT          748 a         1877 c         1719 g          772 t
ORIGIN      
        1 gcgatccgcg cctgagcggc cagcgcgtcg gcttcgggcc ccgccggcgt ggagcgcaat
       61 gcctcgaggt aatggctggc caggacgccg gccagctcgt cgtcaccgag cgcctcgtaa
      121 tagcgggctg cggccaggtg cctgttccgc cgatccgcct tggacagggt ttcgtacgcc
      181 acctcgcgca cgaccgcctg cacgaaccgg tactgacccc gctccggcga tcgggggtcg
      241 tcgtcgcgga tcaggagctc gcgctgaacc aggcggtcga gtccgggctc caccatctcg
      301 aggtctcgac cgcccatgcc ggccatcgcg gcagcggtga agctctgccc caggacggcc
      361 ccatcggcga ggagggaccg atcctccggc gcgttcgcgt cgatccgcgc cgcgaccagg
      421 gcgtggagcg tttcggggac ggccagccgg tcgatcggtg cggtcagggc gtagcccccg
      481 tccgacgcct cgagctggcc acgatcgatc agcatccgaa cggtctcgac cgcgtacagg
      541 gggacgccct cggcccgggc gacgatggcc gtcaccgcgt cctccgggag gcccggcgcg
      601 agaccgcgca gcaggtccgc catggcggcc tcggtcagcg gctcgagcga gacggtggtc
      661 gcgttccgca cgccgctgcc ccatgcggga tgacgctcga acaggtccgg acgggtcatg
      721 gccaggacgt agatcgggtg cgtcttcgac cacgacagga ggtgctcgat gaactcgagc
      781 aggcccgcat cggcccagtg caggtccttg aacacgagga tgaccgggtc gcgttcggcg
      841 atgcgctgga agaacgtccg ccaggccgcg aacaactcgt cgcgctgccc ggtgggcatc
      901 tcatcgagtc ccagcagccc ggcaaggcgc ggctggaccc agcgccgctc ctccgcatcg
      961 gtcagccatt cgtcaaggca ggccgagagc ttggtcgccg cttcctcgac cgagtccgac
     1021 tcggcaatgc cggcccgctc gcgcaccatc tcggccagcg cccagaagct gatcccctcc
     1081 ccatacgacg gggagcggcc cgagtgccag taggcgtcga tcgtcacgcc gtcgatgtat
     1141 ttctcgaatt cccagcccaa ccggctcttt ccaatgccgg cctggccgac aatcgtcacc
     1201 agccggggct tctgctcacg gatcgtggcg tggaacaggt ccttgacgag gcgcagctct
     1261 tcgtcgcggc cgacgaacgg cgcttcgagt gcagaccgtc gtcccgctcc gccgcgcatc
     1321 ccgagcacgc tgaccgcccg ccaggccggg atgggcgagg acttcccctt cagcagctgc
     1381 tcgccggcct cctcgtaggc gatagcctcc gcggttgccc ggtgcgtgga ctcgccgacc
     1441 agcacggtgc cgggcggggc caggctctgc aggcgcgagg ccgtgttgac caggtccccg
     1501 gccacgatcc cctgtccggc cgcgccgatg gtggcggcgg cttcgccggt cagcaccgcg
     1561 gcccgcactt ggagcggctg cccctggtcg atgatggtac cgaccgatga gacgaggtcc
     1621 agggcggcgc ggaccgcgcg ctcggcgtca tcctcgtgcg ccaccgggac gccccagacg
     1681 gccatcaccg cgtccccgat gaacttctcg accgtcccgc cgtagcgcgc gatgacctcc
     1741 gaagcggtat cgaagtaccg ggtcaggagg ttgcgcgtct cctcagggtc gcgatcctcc
     1801 gcgagcgtgg tcgagccgac caagtccgcg aacaggaccg ataccagccg ccgctcggca
     1861 cttgggcctg gttgttctgc ccctgcgatg gcggccggcg ccgccatcgc ctcggcggct
     1921 cgagccggcg cggtatcaga gagaagggcg ccgcattcgt tgcaaaactt ggcccccggc
     1981 gagttctggg taccgcatga cggacatccc gatgccagcg gcgacccgca ctcgttgcag
     2041 aacttcgcgc cgccgacgtt ctcggtgccg cacacggggc aggtcaacga gcttcgacct
     2101 cgctgacgct gtcgcgggca aaggccgccg aagccgcgcg tgcgccactg gccggtgccg
     2161 gcgtgccctg gaacgactga cggtagcggt cgaccacccc ctcagcccct cgcgaggcga
     2221 agaactcggc ggcttcctcg ccggcgcgcc gggcgtcttc aaaggcggcg ccaaggaacg
     2281 catcgaactc aaggccgatc cacgaggtcc acaggttcga ctccaggccc tggtacgtgg
     2341 cgactgtgtc ggcataggcc atgcgcgcct cctcccatcg tgccgccaaa accgcgtcgc
     2401 tggctgccat cttctgtcgc agtccccgcg tccacgtcgc ctgctccggg aagtcgaact
     2461 cgacgggaat cgcggcatgc gcccccacgt cgccaagaag ggcggtggcc agggaccgcg
     2521 tcattgccag gtacaggtcg ttgccgcccc ctgccagtcc ggcgacgctc tcgagggcag
     2581 cccggggatc acccagggca agcagtgcct gcgcctcgac gatcctgatc ccgccgagct
     2641 gcggggcgag catgtcctcg cccgccagga cgcgcgccga atcgtctgcg gccgcccgat
     2701 aggcgtccgc gtcgccgaca accgccgcga gcaacccgcg aatcatcgtc acccatcccg
     2761 cgacgtcggg tggcgacgcg cgctcgtcca cttcggccac gtccaatgcg gctccggccc
     2821 attcccccgt gtacatccgg atctcggccc tggaggtgag gaagactccc agccagctcg
     2881 cgaggccgaa ccggcgagcc aggtcgatgc cgtcgctgac cacctcggcc gcctcgtgat
     2941 agggccgatc gaagagcagg ttcgaggacc agttgttacg cgcacgcagc gctgatgtga
     3001 cgtggccctc gcgatcggcc agctggatgg caccccggaa caccgcgtct ccctcatcgg
     3061 ggcgcttgta ccagagggct gtcgcctgcg tagtcagcgc ttcaaccacg acttccgtgt
     3121 cgcgagacgg gcccgcggcc cgcagcgcgc gttccgccgt ctcgatggcc tcgtcgttct
     3181 ggtcggtcag catgtagagc cgggccagtt cggcaaacac ccgcgcggct tcgggctcgt
     3241 cctcggccat ggtggccgcc accggctcca gggtcccgat gccgtccgat ttgccgtagt
     3301 cggtctggat cctcgccgtc agggccaggg cacggttcgc ggtcgctcgg tcatcgccgg
     3361 cactcgcgag ctcagctgcg gtctgggcat gctcgatcgc ctccggcagg gccaacagcg
     3421 acccggagcg tgccgcgagc tcgtggagcg ccgcctgctc gaggggatcg gtcgtgatcg
     3481 caatggcgtc ctcggcgtgg tggtaggcgg cggcgtacga gtgaagcgcc tccgcccgtt
     3541 cggccgcggc cctcagcgcg atccgggcct ggatggccac ggcgtcggcc tcgggtcccg
     3601 gattggtggc ccgcaatgcc tcgaggtagt ggctggccag gacgccggag atctcgtcgt
     3661 cgccgagggc ctcgaagtac cgagctgccg ccaggtgctt ggctcggcgg tccgccttgg
     3721 ccatggtctc gtaggcaacc tcccggacga ccgcctggac gaaccgatgt tggccgcgct
     3781 cgggcgaccg cgggtcgtca tcgcggatga gcagctcgcg ccggaccagc cggtcgagcc
     3841 ccggcgtgag agtggactcg tcctgtccgg tgacccccga cagggcgtcc atcgtaaagc
     3901 tttgcccgag cacggcggcg tccgccagga cggcacgatc ctccggcgta ttggcgtcga
     3961 tgcgcgccgc gaccagcgcg tgcagcgtct ccggcaccgc cagccggtcg atcggaccgg
     4021 cgagcacgaa tccttcgtcg gtggactgca gctggccacg atcgagcagc atccgaaccg
     4081 tctcgaccgc gtacaggggc acgccctcgg cgcgctgcac gaccgcgtcg accgcggcgt
     4141 cgggcatccc gggcaccagg ccgtgaagca gctctcgcat cggtccctcg ggcagaggct
     4201 cgaggaccac cgtcgtcgca ttgcgcacac cgctactcca gttcgggtgg cgctcgagca
     4261 ggtcgggccg ggtcatcgcg acgacgtaga tcggatggtt cttcgaccag gcgagcacat
     4321 gctcgatgaa ctcgagcagc cccgcgtcag cccaatggag gtccttgaag acgaggatga
     4381 ccgggtcgtg gtcggcgatc cgctcgaaga acgtccgcca ggcggcaaac aggtcctcgc
     4441 gctgggtcgc cggcagctcc tccaggccga gcaggccggc aacccgcggc tcgacccacc
     4501 ggcgctcttc ggagtcgatc agccactggt ccaggcacgc cgtcagcttc gttcgcgcgg
     4561 tcggtacgtc gtcggtctcc gcgatccccg ctcgctcacg gaccatctcg gccagggccc
     4621 agtagctgat gccttcgcca taggacggtg accggccggc gtgccagtag aagtcctggt
     4681 tcacgccatc gatgtacttc tcgaactccc accccagccg gctcttcccg atgcccgcct
     4741 ggccgatgat cgtgaccagc cgcggcttcg actcacggat ggtggcgtgg aacagctcct
     4801 tgaccagccg cagctcctcg tcgcggccca cgaacggcgg ctcgagcgcg gaccggcggc
     4861 gagctccgcc ccgcatgcca aggacctgca ccgctcgcca ggccggcacg gggctcgact
     4921 tgcccttcag cacctgatcg ccaacgtcct cgaaggcgat ggcgtcacgc gtcgcccggt
     4981 aggtcgactc cccgacgagg accgtgcccg cgggcgccat tccctgcagc cggcttgccg
     5041 tgttcaccag gtccccagcg acgatgccct ggccgaccgc cccgatcgag gccgccgctt
     5101 caccggtgag caccgc
//
LOCUS       DASYGU010000208        11922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1628332, whole genome shotgun sequence.
ACCESSION   DASYGU010000208 DASYGU010000000
VERSION     DASYGU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628332"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGO32_05740"
     CDS             <1..439
                     /locus_tag="VGO32_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200288.1"
                     /translation="EIGVLLPPWWLTDADRLLRYRLGQIQAHPEAEPWLIRPIAIRSD
                     PAQAVGITNFHGPPDERGFAEIGYSLHPEFRGQGYAIEAVRAMFGWAAANHGVSRFRA
                     SIAPDNDRSVNLVTKLGMTRVGVQWDEDDGLELIYTVEGWGTA"
     gene            complement(436..1356)
                     /gene="panB"
                     /locus_tag="VGO32_05745"
     CDS             complement(436..1356)
                     /gene="panB"
                     /locus_tag="VGO32_05745"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV7200289.1"
                     /translation="MTTEPQAAESRFTLPELAAMKARGDKIVMVTAYDAPGAHLADQV
                     GIELILVGDSAAMVVLGHDSTVPATVDEMMVLTRAARRGASRALLVADMPFGSFQVSD
                     AEAVGNAIRFVKEADADAVKLEGGGASLSRVRAIVGAGIPVMGHIGLTPQSATMLGGY
                     KAQGRTAAKARALFADAQALQDAGCFAIVLEAVPAPVATRISQALRIPTIGIGAGVGC
                     DGQVLVWHDLLGLTEGRAPRFVKRYGHLAEDIRAALATYAADVRSGEFPDDEHSYGIP
                     DEELALFESELDSGVLVQAEDARGSGSDWL"
     gene            complement(1360..2187)
                     /gene="panC"
                     /locus_tag="VGO32_05750"
     CDS             complement(1360..2187)
                     /gene="panC"
                     /locus_tag="VGO32_05750"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV7200290.1"
                     /translation="MKVVRTALELRGALAERRGKGDVGLVPTMGAFHDGHRSLFDAAR
                     AECGTVVASLFVNPAQFAPGEDYAAYPRDEPRDVAAAEAASIDVLFIPDRTEIYPPGF
                     DTWVDPGALGEMLEGTVRPGHFRGVATVCLKLFNLVGAQRVYFGRKDAQQVAVIRAML
                     RDLDLEAAITLRVLPTVRDTDGLALSSRNAYLSTEERQRALALPRALDAGLAAHRAGG
                     DPVDAARAVLDAADGVEVDYVAVAAFDGPTLAAAIRMGRTRLIDNVLLKDEDADGGG"
     gene            complement(2184..2882)
                     /locus_tag="VGO32_05755"
     CDS             complement(2184..2882)
                     /locus_tag="VGO32_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEV7200291.1"
                     /translation="MFGAIHVVGTGRAGTALAGRLRAAGSTVSTGRVVERTADVVLIA
                     VPDGAIADVAASVPLGPWVAHVSGATSVTALAPHTRRFCLHPLQTLTLDRGPDQLDGA
                     WAALTFESEEAGDAARWLAGRLGLQPFELADSDKALYHAGAAMASNFLVTLYRAAARL
                     FIESGAPPEALVPLMRRVIENGFQLTGPIARGDWATVDAHLAALAERAPDVEPVYRAL
                     AEATRTIAERDADG"
     gene            complement(2927..3451)
                     /locus_tag="VGO32_05760"
     CDS             complement(2927..3451)
                     /locus_tag="VGO32_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200292.1"
                     /translation="MAAVSQGETSVEWYPWIVTAHVLGAFLFVFGHGASAFAALRLRR
                     ERDPVRIGAMLDVSVTSYAAMGVGWLLLLIAGIVATFMAGLWGQGWIWVSLVMFVLLT
                     LYMGARAGEWMRGIRRGIGMKPPFSKPEVEGPTPVTAQELDRMLQSPRMFEVTLVGSV
                     GLVVIIGLMVLKPF"
     gene            3509..4408
                     /locus_tag="VGO32_05765"
     CDS             3509..4408
                     /locus_tag="VGO32_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV7200293.1"
                     /translation="MSGPTFLNLPAALPETTDVAIAILGVPFGVPYPKPGTAAGCAAA
                     PAAIRQASRRFASAVAHHDFDTGQPMVPRPERIADCGDVTGDPSDGAGNVARTEAAVR
                     TLHERGAMPIVFGGDDSIPIPVLAAFADRGPITVLQVDAHLDYRDEVQGVRHGYSSPM
                     RRASEMAHVERIVQVGLRGVGSARPSDVADARAAGGLLVTARDLRQRGVAWVFDQLPA
                     GASVFISFDLDGLDPSLCPAVSAPAPGGLSWDEAGDLLSGAATRCRVVGAAFTELVPE
                     RDPSGYSALVAARLGIRLLSAIP"
     gene            4405..5448
                     /locus_tag="VGO32_05770"
     CDS             4405..5448
                     /locus_tag="VGO32_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200294.1"
                     /translation="MSVSGGPAADSLAIEATDLVRTYRTHLGVIRRHSIDVEAVRGIS
                     FNVRNGELFGLLGPNGAGKTTTIKMLVTLLLPTAGSARVMGYDVVADTTEVRKRIGYV
                     FGGERGLYDRISGLDNLRFFAELYGVEPDAQRPRIDALLDLVGLAGRENEKVEGYSRG
                     MRQRLHIARGLLHDPPVLFLDEPSIGLDPVGARELRKTISTLVQAGKTILLTTHYMFE
                     ADALCERIAVINGGKIVAAGTGSDLKAVIANRTIVEIETYGVTTEAVDRLRADPDVIA
                     VNTEDQDQSQILLVQSERGTEITQRLIQQLGDTRIGKISAREPTLEDAYVHLIASTGG
                     EDGNGAARAAAVA"
     gene            5452..6255
                     /locus_tag="VGO32_05775"
     CDS             5452..6255
                     /locus_tag="VGO32_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200295.1"
                     /translation="MVSLAELRSNLHQVGRVWVFQIRIFLISDFFILTAVVQPVIFAT
                     IAFLLFQVGGESAGEQTLLFAALGTGMMGVWSTTLFGGGGAIAWQRWESTLELLVAAP
                     RRYDLTLLGQTLATTTFGFYSMVATLAWGVLLFGMPLNANYPLMLPLSLLAAVIGLGI
                     MGMLLATTFVLYRHANALSNLLEYPVWIATGMLVPLALLPGWLGPISWVLLPTWAVEA
                     IRGAAIGTEPPWLPIGMCIVLALIYYVIARRVLVYVIDKARRDATLNLA"
     gene            6262..7095
                     /locus_tag="VGO32_05780"
     CDS             6262..7095
                     /locus_tag="VGO32_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200296.1"
                     /translation="MSAIPVSTVPSQPGSLRANWRIFWNGGRISYAALFNWARPSIYI
                     PTLLGAPTFQILYFAALGSYDTGMDPEFFAIGNAVQVCSMAGIYGMTMAVANERWFGT
                     LPALMASPANRAALFGGRFIPFIVNGFLVSAYGFVLCWIFLDVRFDAATLGVMVLAMI
                     VTAFSCSAIGAVMGGVSLRLRDGLFGANLLVFLFLLFCGVNIPLSALPDWMQAVSQVL
                     PFTHGLAAVRLAIDGATLAQVGGLIATEFFIGVAYAVAAFFLFTWLERSSYRNATLDA
                     R"
     gene            complement(7109..8119)
                     /gene="corA"
                     /locus_tag="VGO32_05785"
     CDS             complement(7109..8119)
                     /gene="corA"
                     /locus_tag="VGO32_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEV7200297.1"
                     /translation="MTDTAGSERPRRLALALMPDGTLETLDESAYDRIDRLITKDDTL
                     VWLDIGDPTNDDLALLTREFGLHPLAVEDLRKRRQRPKVDTYPGHHVIVAYEALPAND
                     SMRAFDLGELHLFAGRQFVVSVHWGRSPIIEAVQERFQQRTSAVGPSAGWLLYAILDT
                     AVDGYFPLLDRISEEIDDLEDRILGGKGNPVLLREVLHLKRQLLELRRVLGPMRDSAN
                     QLLRREVEILDDEALPYYQDLYDHLVRVLDSLDLYRDLVSATLDAHESVTTNNLNQIV
                     KRLTAVTVLLMVPTLIAGIYGMNFFFMPELELRYGYPAVLLGMLIIVIVLGWWMRRRG
                     WF"
     gene            complement(8116..9447)
                     /locus_tag="VGO32_05790"
     CDS             complement(8116..9447)
                     /locus_tag="VGO32_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200298.1"
                     /translation="MPFDVAPWVAIGALVLAVLPLLSAGAEKEILAVSSADMVERNVP
                     TTVSYVQLTGLAMSIQLPAPGGSARPPYGLLVRDAVGSDQMTVVMTDSDPLQLRVRAV
                     VARVQAMPYSDAAAAAFTARGDASAGLGSGPVLVEASPSSDETVSDISSAAEVATLPN
                     GALVRLPLRLDGEAVAICALADGGCADRVLARGEGVFAQLAHDNEGIPILVQTIAPTS
                     AVAGTWQGAQVRNEDDLGEFAESLPVRAMAGWGRILVLASIQDDPALERDRSWLGPVL
                     LLILSVLLWLGGRVGYPSFRPALEGARGWSRPTVGGTTTESPDESPGRELAEIAVVVS
                     GHGLTTDGRRRHLDETIATLRPADSAESDGRLTAALVLADSSRLVLAAHEAGRLGSVE
                     RGDVTSLTGVRPALWAHWYGTDLRMNFASAADRDRAAELISGGGPSVRSSS"
     gene            complement(9535..9954)
                     /locus_tag="VGO32_05795"
     CDS             complement(9535..9954)
                     /locus_tag="VGO32_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200299.1"
                     /translation="MSWTQLAGQVGSTPAVTSWAENEMQVFAIGPDGALSSIYWDGAA
                     WHDWHGHGGAFVGSPTACSWGPDRIDVFARRADGTVMHQWYAPDGWSAWESLGVTADG
                     DLAACSWGRNRIDLFARGAAGQLLHGVWDGSTWSFGD"
     gene            10039..11415
                     /locus_tag="VGO32_05800"
     CDS             10039..11415
                     /locus_tag="VGO32_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEV7200300.1"
                     /translation="MDRWRTIIEPFRIKSVEPIRLTTVAEREAALQAAGYNLFNLHAE
                     DVLIDLLTDSGTGAMSAEQWAGIQRGDESYAGSPSWFRFLEAVQELFPFKHVIPTHQG
                     RAAEKILFSVVAGPGTVVPNNTHFDTTRANVEFTGAEAADLPIPEGQQPATIHPFKGN
                     MDVSALERLIQERGPDAIPVVFVTITNNSGGGQPVSLANLRDVRAVCDRYDLPLFLDA
                     CRFAENAWFIRTREAGYEATSIPDIVREMASLADGMTMSAKKDGLANMGGWLALNDDR
                     WAEQARNLLILTEGFPTYGGLAGRDLDSIAVGLREVVDEDYLRYRIRSTEYLGDGVTA
                     AGIPCIQPIGGHAVYLDAAALLPHIPALEYPGQALAVALYREGGIRGCEIGTVMFGRH
                     PDGTETPAPMELVRLAIPRRVYTQSHVDYVIEVARWVADRAADLRGFLITEQPSALRH
                     FTARFEPL"
     gene            11561..>11922
                     /locus_tag="VGO32_05805"
     CDS             11561..>11922
                     /locus_tag="VGO32_05805"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200301.1"
                     /translation="MLAHAAAHPERLVLGIDASHAAMREASRRAARAPARNGRPNALF
                     VASALESLPPDLAGVADLVMVSFPWGTLRTAAAGHDRDLTARLAGLIGPGGRLELLLA
                     DAERDGTPPVDLEALRDT"
BASE COUNT         1785 a         4277 c         4045 g         1815 t
ORIGIN      
        1 cgagatcggg gttctcctgc cgccctggtg gctgaccgac gccgatcgtc ttctccgcta
       61 tcggctcggc cagatccagg cccatcccga ggccgagccc tggctgatcc ggcccatcgc
      121 catccgttcc gaccctgccc aggcggtcgg gatcacgaac ttccatggac cgccggacga
      181 gcgcggcttc gccgagatcg ggtattccct ccacccggag ttccggggcc aggggtatgc
      241 gatcgaggcc gtgcgcgcga tgttcggctg ggcggccgcg aaccacgggg tcagccggtt
      301 ccgggcatcg atcgcgccgg acaacgaccg gtccgtcaat ctcgtcacca agctggggat
      361 gacccgggtc ggcgtgcagt gggatgagga cgacggcctg gagctgatct acaccgtgga
      421 agggtgggga acggcctaga gccagtcgct ccccgagccg cgtgcatcct cggcctggac
      481 cagcaccccg ctgtccagct cggactcgaa cagggccagc tcctcgtccg ggatgccgta
      541 cgagtgctcg tcgtcgggga actcgccgct gcggacatcg gcggcgtagg tggcaagcgc
      601 ggcgcggatg tcctcggcga ggtggccgta gcgcttcacg aagcgtggcg cgcggccctc
      661 ggtcagtccg agcaggtcgt gccagaccag gacctgtccg tcacatccga cgcccgcccc
      721 gatgccgatg gtcgggatgc ggagggcctg gctgatccgg gtcgcgaccg gcgcgggcac
      781 cgcctcgagg acgatcgcga aacagccggc atcctggagg gcctgggcgt cggcgaacag
      841 ggcgcgggcc ttggccgcgg tacgaccctg ggccttgtag ccgcccagca tcgtcgccga
      901 ctgcggcgtg aggccgatgt ggcccatgac cgggatgccg gcgccgacga tggcccgcac
      961 gcgggacagc gaggcgccgc cgccctcgag cttaaccgca tcagcatcgg cttccttgac
     1021 gaaccgaatc gcgttcccga ccgcctctgc gtccgacacc tggaacgagc cgaagggcat
     1081 gtccgccacc agcagcgcac gactggcgcc gcgccgggcg gcccgggtga ggaccatcat
     1141 ctcgtcgacc gtggccggca ccgtggagtc gtggccgagg acgaccatcg cggccgagtc
     1201 accgaccagg atgagctcga tcccgacctg atcggccagg tgggcgcccg gcgcgtcgta
     1261 ggcggtgacc atcacgatct tgtcgccgcg ggccttcatg gccgccagct cgggcagcgt
     1321 gaagcgcgat tcggcggcct gcggttcggt cgtcatcggt catccacctc catcggcatc
     1381 ttcgtccttc agcaggacgt tgtctatcag acgggtgcgc cccatccgga tcgcggcggc
     1441 cagggtcgga ccgtcgaacg cggcgaccgc gacgtagtcc acctcgaccc catcggcggc
     1501 gtccaggact gcccgcgcgg cgtccaccgg atcgcccccg gcacgatggg cggcgagccc
     1561 ggcgtccagc gctctcggga gcgccaatgc gcgctgccgt tcctcggtcg agaggtacgc
     1621 attgcgcgaa gacagggcaa ggccgtccgt gtcgcgcacg gtgggcagta cgcggagggt
     1681 gatggccgcc tcgaggtcga gatcgcggag catggcccgg atgacggcca cctgctgcgc
     1741 gtcctttcgg ccgaagtaca cgcgctgggc gccgaccagg ttgaacagct tgaggcacac
     1801 ggtggcgacc ccccggaagt ggccgggccg gaccgtccct tccagcatct cgcccagcgc
     1861 ccccggatcc acccacgtgt cgaaccccgg cgggtagatc tcggttcggt cggggatgaa
     1921 caggacgtcg atggatgccg cctcggccgc ggccacgtcg cgtggttcgt cccggggata
     1981 tgccgcgtag tcctcgcccg gcgcgaactg ggccgggttc acgaacagcg aggcgaccac
     2041 ggttccgcat tcggcccgcg cggcgtcgaa cagcgaccgg tgaccgtcat gaaaggcgcc
     2101 catggtcggg accagcccga cgtctccctt gccacgccgc tcggccagcg cgccacgcag
     2161 ctcgagggcg gtccggacga ccttcatccg tccgcgtctc gctcggcaat tgtcctggtg
     2221 gcctcggcga gggcgcgata caccggctcg acatcaggag cccgctcggc cagtgccgcg
     2281 aggtgcgcgt ccacggttgc ccagtcgccc cgggcgatgg gtcccgtgag ctggaatccg
     2341 ttctcgatca cccgacgcat gagcgggacg agcgcttccg gcggcgcccc gctctcgatg
     2401 aacagtcgcg cggctgcccg gtacagcgtg accaggaagt tggatgccat cgcggctccg
     2461 gcgtgataga gcgccttgtc cgaatcggcc agctcgaacg gctggaggcc caagcgaccg
     2521 gccagccagc gggccgcgtc gccggcctcc tcggactcga aggtgagagc cgcccacgcc
     2581 ccgtcgagct gatccggtcc gcgatccagg gtgagggtct ggagcggatg gaggcaaaac
     2641 cgacgggtgt gcggcgccag cgcggtgacc gacgtcgcgc ccgacacgtg ggccacccac
     2701 ggaccgagcg gcacggacgc ggcgacgtcc gcgatggcgc catcgggaac ggcgatcagg
     2761 accacgtcgg cggtccgctc cacgacacgt ccggtgctga cggtcgagcc ggctgcacgg
     2821 agacggccgg ccagggccgt gcccgcccga ccggtgccca cgacgtggat cgctccgaac
     2881 acgcccgcag tctagtcggg caccgcgacg ggtcgcccga cgccggtcag aacggcttga
     2941 ggaccatcag gccgatgatc accaccaggc ccacgctgcc gacgagggtc acctcgaaca
     3001 tgcgaggcga ctgaagcatc cgatcgagct cctgggcggt caccggagtc ggaccctcga
     3061 cctccggctt ggagaacggc ggcttcatac cgatcccccg gcggatgccg cgcatccact
     3121 ccccggcccg cgcacccatg tacagggtca gcagaacgaa catgacgagc gagacccaga
     3181 tccatccctg gccccacagg ccggccatga acgtggccac gatccccgcg atcaggagca
     3241 gcagccatcc aacgcccatc gccgcgtagc tggtgaccga cacgtcgagc atggcgccga
     3301 tccggactgg gtcccgctca cggcgaagcc ggagcgcggc aaaggcggac gcgccgtgtc
     3361 caaatacgaa caggaacgcg cccagcacgt gggcggtcac gatccacgga taccactcca
     3421 ccgacgtttc tccctgcgat acggcggcca tcttgccgcc tccgcggcgg gagccgcagg
     3481 gatttcgcgt ggtgtacggt tggtcgccgt gtccggccca accttcctga atctccccgc
     3541 cgccctgccc gagacgaccg atgtcgcgat cgccatcctc ggcgtcccgt tcggggtccc
     3601 atacccgaag ccaggcaccg cggccggttg cgcagccgct ccggccgcga tccggcaggc
     3661 gtccaggcgc ttcgcgtcag ccgtcgctca tcacgacttc gacaccggcc agccgatggt
     3721 gccgcgtccc gagcggatcg ccgactgcgg cgacgtcacg ggcgacccgt cggacggtgc
     3781 cggcaatgtc gcacggacgg aggccgcggt ccggaccctg cacgagcggg gggccatgcc
     3841 gatcgtgttc ggtggcgacg actcgatccc gatcccggtg ctcgccgcgt tcgcggatcg
     3901 aggacccatc accgtgctcc aggtcgatgc ccatctcgac taccgcgacg aggtgcaggg
     3961 cgtccggcac ggctactcgt cgcccatgcg tcgcgcgtcg gagatggccc acgtcgagcg
     4021 gatcgtgcag gtcggcctgc gcggggtcgg cagcgcgcgc ccgtcggacg tggccgatgc
     4081 ccgagcagcc gggggcctgc tcgtgaccgc gcgcgatctc cgtcagcgag gcgtggcgtg
     4141 ggtcttcgac cagctccccg ccggcgcgtc ggtcttcatc tcgttcgacc tcgacggcct
     4201 ggatccaagc ctctgtcccg ccgtcagtgc gccggcgccg ggcggtctgt cgtgggacga
     4261 ggccggtgac ctgttgtccg gcgcggcgac ccggtgccgg gtggtcggcg ccgcattcac
     4321 ggagctcgtc cccgagcgtg acccgagcgg ctattcggcc ctggtcgccg cccgcttggg
     4381 tattcggcta ctctcggcca tcccatgagc gtttccggcg ggccggctgc cgattccctg
     4441 gccatcgagg ccaccgacct ggtccgcacc taccgaaccc atctcggcgt gatccgccgc
     4501 cactcgatcg acgtcgaagc ggtgcgcggg atcagcttca acgtccgaaa cggcgagctc
     4561 ttcggcctgc tcgggccgaa cggcgcgggc aagacgacca cgatcaagat gctcgtcacg
     4621 ctcctcctgc cgaccgcggg ctcggcacgg gtcatgggct acgacgtcgt cgccgacacg
     4681 accgaggtgc gcaagcggat cggctacgtg ttcggcggcg agcgcggcct gtatgaccgg
     4741 atcagcggcc tcgacaacct ccgcttcttc gccgagctgt acggcgtcga gccggacgcc
     4801 cagcggcccc ggatcgacgc cctgctcgac ctcgtcggcc tggccggccg ggagaacgag
     4861 aaggtggagg gctactcgcg aggcatgcgc cagcggctgc acatcgcgcg cggcctgctc
     4921 cacgacccgc cggtcctgtt cctcgacgaa ccatccatcg gtcttgaccc ggtgggggcg
     4981 cgcgagctgc gcaagacgat ctcgaccctc gtccaggccg gcaagacgat cctgctcacc
     5041 acccactaca tgttcgaggc cgatgcgctc tgcgaacgga tcgcggtgat caacggcgga
     5101 aagatcgtgg ccgccggcac cgggtcggac ctgaaggcgg tcattgccaa ccggacgatc
     5161 gtcgagatcg agacctacgg cgtcacgacc gaggccgtcg atcggctgcg cgccgacccg
     5221 gacgtcatcg cggtgaacac cgaggaccag gaccagtccc agatcctcct cgtccagtcc
     5281 gagcgcggga ccgagatcac gcaacggctg atccagcagc tgggcgacac ccggatcggc
     5341 aagatctccg cccgcgagcc caccttggaa gacgcctacg tccacctcat cgccagcacc
     5401 ggcggcgagg acggcaatgg ggctgcccgc gcggcggcgg tggcctgacc gatggtgtcg
     5461 ctggccgagc tccgatcgaa cctgcaccag gtcgggcggg tctgggtctt ccagatccgg
     5521 atcttcctga tcagcgactt cttcatcttg accgcggtcg ttcagcccgt gatcttcgcg
     5581 accatcgcct tcctcctgtt ccaggtcggg ggcgagtccg cgggcgagca gaccctcctc
     5641 ttcgcggcgc tggggaccgg gatgatgggc gtctggtcca ccaccctgtt cggcggcggc
     5701 ggagccattg cgtggcagcg ctgggagtcc accctcgagc tgctggtcgc cgctccccgg
     5761 cgctacgacc tcaccctcct gggccaaacc ctggccacga ccacgttcgg gttctactcg
     5821 atggtcgcca ccctggcctg gggcgtgctc ctgttcggca tgcccctcaa cgcgaactat
     5881 ccgctcatgc tgccgctgtc cctgctggcc gcggtcatcg gcctcgggat catgggcatg
     5941 ctgctggcca cgaccttcgt gctgtaccgc catgccaatg cgctcagcaa cctgctcgag
     6001 tacccggtct ggatcgcgac cggcatgctc gtgccgcttg ccctgctccc gggctggctg
     6061 ggcccgatca gctgggtgct gctcccgacc tgggcggtgg aggccatccg cggcgcggcg
     6121 atcggcaccg agccaccgtg gctgccgatc gggatgtgca tcgtcctggc gctgatctac
     6181 tacgtcatcg cccgcagggt cctcgtctac gtcatcgaca aggcgcggcg cgacgcgacc
     6241 ctcaacctgg cctgatccgc cgtgtccgcg attcccgtct ccaccgttcc ctcccagccg
     6301 ggctcgctcc gcgcgaactg gcggatcttc tggaacgggg gtcggatcag ctatgccgcg
     6361 ctgttcaact gggcgcggcc ctcgatctac atccccaccc tgctcggcgc gccaacgttc
     6421 cagatcctgt acttcgcggc actcggcagc tacgacaccg ggatggatcc ggagttcttc
     6481 gccatcggca acgcggtcca ggtgtgctcc atggccggga tctacggcat gaccatggcc
     6541 gtcgcgaacg agcgctggtt cggcacgttg cccgccctga tggccagccc cgcgaaccgg
     6601 gccgccctgt tcggcgggcg gttcatcccg ttcatcgtga acgggttcct tgtctcggca
     6661 tacggcttcg tcctgtgctg gatcttcctc gacgtccgtt tcgacgccgc caccctgggc
     6721 gtgatggtgc tggccatgat cgtgaccgcg ttctcgtgtt cggctatcgg cgcggtgatg
     6781 ggtggcgtgt ccctccggct gcgcgacggc ctgttcggcg ccaacctgct cgtgttcctg
     6841 ttcctcctgt tctgcggcgt gaacattccg ctttccgccc tgcccgactg gatgcaggcc
     6901 gtgagccagg tgctcccgtt cacgcacggc ctggccgccg tccggctcgc gattgacggt
     6961 gcgaccctgg cccaggtcgg cggcctgatc gcgaccgagt tcttcatcgg cgtcgcctac
     7021 gccgtggccg cgttcttcct gttcacctgg ctcgagcgaa gctcgtaccg gaatgccacc
     7081 ctcgacgccc gttgacggag ggcggtccct agaaccagcc tcggcgacgc atccaccagc
     7141 cgagaacgat gacgatgatg agcatcccga gcagcaccgc cgggtagcca tatcgcagct
     7201 ccagctcggg catgaagaag aagttcatgc cgtagatacc cgcgatgagg gtgggcacca
     7261 tcaggaggac cgtgaccgcg gtcaggcgct tcacgatctg gttgaggttg ttggtcgtga
     7321 ccgattcgtg ggcgtcgagc gtggccgaca ccaggtcgcg atacaggtcg agcgagtcga
     7381 ggacccggac caggtggtcg tagaggtcct ggtaataggg cagggcttcg tcatcgagga
     7441 tctcgacctc gcgacggagg agctggttgg ccgagtcgcg catcggtccc agcacccggc
     7501 gcaactcgag gagctggcgt ttcaggtgga ggacttcgcg gaggagcaca gggttgcctt
     7561 tgccgccgag gatccggtcc tccaggtcgt cgatctcctc cgagattcgg tccaggagcg
     7621 ggaagtagcc gtcgaccgcg gtgtcgagga tcgcgtacaa cagccagccc gcactgggtc
     7681 ccaccgctga cgtgcgctgc tggaagcgct cctggacggc ctcgatgatc ggcgaccggc
     7741 cccagtggac actgaccacg aactggcgcc cggcgaacag gtggagctcg ccgaggtcga
     7801 acgcccgcat cgagtcgttg gccggcaacg cctcgtacgc gacgatcacg tggtggcccg
     7861 ggtaggtgtc gaccttgggc cgctgccggc gcttgcggag gtcctcgacg gccagggggt
     7921 gcaggccgaa ctcgcgggtg aggagggcga ggtcgtcgtt ggtcgggtcg ccgatatcga
     7981 gccagaccag cgtgtcgtcc ttggtgatga gcctgtcgat cctgtcgtac gccgactcgt
     8041 ccagcgtctc gagcgttcca tcgggcatga gcgccagtgc gagccgccgc ggtcgttcgc
     8101 tgcccgccgt atcggtcatg acgacgatcg taccgagggc ccgccgccgc tgatgagctc
     8161 cgccgcgcga tcgcggtcgg ccgccgaggc gaagttcatg cgcaggtctg tcccgtacca
     8221 atgcgcccac agggccgggc ggacgccggt cagggacgtg acgtcccctc gctccacgct
     8281 gccgagccgg ccggcttcgt gggcagccag cacgagccga ctgctatcgg ccaggaccag
     8341 ggcagcggtc agccgcccat cggactccgc gctgtcggct ggtcggaggg tggcgatggt
     8401 ttcgtccagg tgccgtcgcc ggccgtccgt ggtcaggccg tggccgctga ccaccactgc
     8461 gatctcggcc agctccctgc ccggcgattc atcgggtgat tcggttgtcg tgcccccgac
     8521 cgtcgggcgg ctccatccgc gagcgccctc gagcgcgggt cggaacgacg gatagccgac
     8581 ccggccgccg agccagagca ggaccgaaag gatgagcagc aggacagggc caagccatga
     8641 ccgatcccgc tcgagggccg ggtcatcctg gatggaggcc aggaccagga tccgtcccca
     8701 gcccgccatc gcgcgaacgg ggagcgattc ggcgaattcc ccgagatcgt cctcgttccg
     8761 gacctgggct ccctgccagg tgcccgcgac agcggacgtg ggcgcgatcg tctggaccag
     8821 gatcgggatc ccctcgttgt cgtgcgcgag ctgggcgaac acgccctcgc cccgcgcgag
     8881 gacgcgatcc gcgcacccgc catcggccag cgcgcaaatg gccaccgcct ccccgtcgag
     8941 ccggagcggg agccggacca gcgccccgtt cggcagggtt gcgacctcag ccgccgagct
     9001 gatatcggaa accgtctcgt cggaggatgg cgaggcctcg accagcaccg gcccgctgcc
     9061 caggcccgcg ctcgcgtcac cgcgggccgt gaacgccgcc gccgccgcgt cgctataggg
     9121 catggcctgg acccgcgcga cgaccgcgcg gacacggagc tggagaggat ccgaatcggt
     9181 catgaccacg gtcatctggt cgctgccaac cgcatcgcgt acgagaagcc cgtagggcgg
     9241 tcgtgcgctc ccgccggggg ccggcagctg gatgctcatg gccaggccgg tgagctggac
     9301 gtaggacacc gtggtgggca cgttgcgttc gaccatgtcg gccgacgaga cggccaggat
     9361 ctccttctcg gcgcccgccg agagcaacgg caggaccgcg aggaccaagg ccccgatcgc
     9421 gacccacggg gcgacatcga acggcaatgg ccggccgacg gcggcccgga cccagcgttc
     9481 gagcgcgacc ggcaggaagc cgatgagggc tcgcagacga tcgatggcga acccctagtc
     9541 cccgaaggac caggtcgagc cgtcccacac gccatggagc agctgaccag cagcgccacg
     9601 agcgaacagg tcgatccgat tccggcccca cgagcaggcg gccaggtccc cgtcggcggt
     9661 cacgcccagt gactcccacg cggaccagcc atccggtgcg taccactggt gcatcaccgt
     9721 cccgtccgcc ctgcgggcaa agacgtcgat ccggtccggg ccccacgagc acgcggtggg
     9781 ggacccgacg aacgcgcccc cgtggccgtg ccagtcgtgc cacgcggcac cgtcccagta
     9841 gatgctcgac agcgcgccat cgggaccgat cgcgaagacc tgcatctcgt tctcggccca
     9901 gctcgtcacc gccggtgtcg atccaacctg cccagccaac tgtgtccagc tcatgaccca
     9961 tggcctcccg tgatcggcgc tcgtgtggag cgacccgaat aatgccccac cgcggcgagc
    10021 cggccgtatc ctgcccgggt ggaccgctgg cgaacgatca tcgagccatt ccggatcaaa
    10081 tcggtcgagc cgatccgcct gaccaccgtc gccgagcgcg aggccgcgct gcaggccgcg
    10141 ggatacaacc tgttcaacct ccacgccgag gacgtcctga tcgatctgct gaccgattcg
    10201 ggcaccgggg ccatgagcgc ggagcaatgg gcgggcatcc agcgcgggga cgagtcgtac
    10261 gccggcagcc cctcgtggtt ccggttcctg gaggccgtcc aggagctgtt ccccttcaag
    10321 cacgtgattc ccacccacca gggccgcgcg gcggagaaga tcctgttcag cgtcgtcgcc
    10381 gggccgggca cggtggttcc gaacaacacc cacttcgaca cgacccgggc gaacgtcgag
    10441 ttcaccggcg ccgaggccgc cgacctcccc attcccgagg gtcagcagcc ggcgaccatc
    10501 cacccgttca agggcaacat ggacgtgtcc gccctggaac ggctcatcca ggaacgcggc
    10561 ccggatgcca tcccggtcgt gttcgtcacg atcacgaaca actcaggcgg cggccagccg
    10621 gtgtcgctgg ccaaccttcg cgacgtgcgc gcggtctgcg accgctacga cctgccgctg
    10681 ttcctggacg cgtgccgctt cgccgagaac gcatggttca tccggacccg cgaggcgggc
    10741 tacgaggcga cctcgatccc ggacatcgtc cgcgagatgg cctccctggc cgatgggatg
    10801 acgatgtcgg ccaagaagga cggactggcg aacatgggcg gttggctggc cctgaacgac
    10861 gaccgctggg ccgagcaggc acgcaacctg ctgatcctga ccgagggctt tccgacgtac
    10921 ggcggtctcg ccggtcggga cctggactcg atcgcggtcg ggctgcgcga ggtcgtcgac
    10981 gaggactacc tccgctaccg gatccgctcg accgagtacc tcggtgacgg ggtgaccgcg
    11041 gccggcatcc cgtgcattca acccatcggc gggcacgcgg tctacctcga cgccgccgcg
    11101 ctcctgccgc acatcccggc gctcgagtac cccggccagg ccctcgccgt cgcgctctac
    11161 cgggaaggtg gcatacgcgg ctgcgagatc gggacggtga tgttcggccg gcacccggac
    11221 ggcaccgaga cgccggcgcc tatggagctc gttcgcctcg ccatcccacg tcgggtgtac
    11281 acccagagcc acgtcgacta cgtgatcgag gtcgcgcgct gggtcgccga tcgggccgcg
    11341 gacctgcgtg ggttcctgat caccgagcag ccgagcgccc tgcgccattt caccgcccgc
    11401 ttcgagccgc tgtgaggccg ccgcgccggg ccagtatcat ccgctcatgc gtctcctgac
    11461 tggacgccac ctcgccgagg tggacgtcag ctaactgacc gataccgccg cggcgtatgc
    11521 cgcgacgacg gtcgacctgg gcaccggtga cggccggttc gtgctggccc acgccgcggc
    11581 ccaccccgag cggctggtgc tcggtatcga tgccagccat gccgccatgc gggaggcgtc
    11641 gcgtcgagcg gctcgggcac ccgcccgtaa cggccggccg aatgccctgt tcgtcgcgtc
    11701 tgcgctcgag agtctgcctc cggacctcgc cggcgtggcc gatctggtga tggtctcgtt
    11761 cccgtggggg accctgcgca cggcggcggc cggccacgat cgtgacctga ccgcccggct
    11821 ggccggtttg atcggaccgg gtggccggct cgagctcctg ctcgccgacg ccgaacgcga
    11881 cggcactccg cccgtcgacc tcgaggcgct gcgggatacc ta
//
LOCUS       DASYGU010000209         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1011157, whole genome shotgun sequence.
ACCESSION   DASYGU010000209 DASYGU010000000
VERSION     DASYGU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011157"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VGO32_05810"
     CDS             complement(<1..469)
                     /locus_tag="VGO32_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7200302.1"
                     /translation="MVRTASAPPRWQVAAALGIVYVVWGSTYLGIAVMVQTLPPLVAA
                     GIRYTAAGVILLALLIVWHRVRGNPLERPNRFHWRSAIIIGTLLLLGGNGGVVLGEQL
                     IATGIAALVIATTPIWMAVFEAIVAGQRPGRLTIAGLGAGIIGVAILVVPLEGS"
     gene            508..1182
                     /locus_tag="VGO32_05815"
     CDS             508..1182
                     /locus_tag="VGO32_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200303.1"
                     /translation="MRILIGLPGAVAVVLLVLLVLPLFGVSIGFGPFASVEPSASASA
                     SASASASSSGSEAASPAESGAESIAPTSTPTPPDEGWICGLPVTLAASGAAIHHTIDV
                     SVSTHPDFDRISFEYQEAGSPAFEMRNTRPPFTKDPSGQPMTVNGSNFMRITLNGATK
                     LADDGGLTYTGPTNFEPGFPQLVQLIEQGDFEALNSWYVGLDGGDCIRAAVVTGPSRI
                     IIDVQH"
     gene            1191..>1686
                     /locus_tag="VGO32_05820"
     CDS             1191..>1686
                     /locus_tag="VGO32_05820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7200304.1"
                     /translation="MTHSPPMGDLDLSKNYSARITTERGAFDVELFAADAPLTVENFV
                     SLARAGFYDGTTFHRVIPGFMAQGGDPTGTGSGGPGYQFRDEFGSSRSHDAAGVLSMA
                     NAGPNTNGSQFFITFGPTPHLDGKHTVFGRVTDGLDVAMGIRVRDPGRDPNPGERIER
                     IEITE"
BASE COUNT          271 a          591 c          568 g          256 t
ORIGIN      
        1 ggctgccttc cagcggaacg acgaggatgg cgacaccgat gattccggcc ccgagcccgg
       61 cgatggtcag gcggcccggc cgctggcccg cgacgatcgc ttcgaagacg gccatccaga
      121 tgggagtggt ggcgatgacg agggccgcga tcccggtcgc gatgagctgc tcgccgagca
      181 cgacgccccc gttcccgccc agcagcagga gcgtcccgat gatgatcgcg gaccgccaat
      241 ggaaccggtt cggccgctcg agggggttgc cgcggacgcg gtgccacacg atgaggaggg
      301 cgaggaggat gacgccagcc gccgtgtagc ggatgccggc cgcgaccagc ggcgggaggg
      361 tctggaccat gaccgcgatg cccaggtagg tggatcccca caccacgtac acgatcccga
      421 gcgcggccgc gacctgccat cggggagggg ctgaggcggt ccggaccatc ggcgcattgt
      481 gcccggcctc ggtagactgc gccgagcatg cgcattctga tcggcctgcc cggggcggtc
      541 gccgtcgtgc tccttgtcct cctcgtgctt ccgctgttcg gcgtgtccat cggctttggg
      601 ccgttcgcca gcgtcgagcc gtcggcgtcg gccagtgcga gcgcgagcgc atcggccagc
      661 tcctccgggt cggaggcggc gtcaccggcc gagagcggcg cggagtccat cgcgccaacg
      721 tccaccccga ccccgccgga tgagggctgg atctgtgggc taccggtgac cctggcggcc
      781 agcggcgcgg ccatccacca caccatcgat gtcagcgtca gcacccaccc cgacttcgat
      841 cgcatctcgt tcgagtacca ggaagccggc tcaccggcct tcgagatgcg caacacgagg
      901 ccgcccttca ccaaggatcc gagcgggcag ccgatgaccg tgaatggctc gaatttcatg
      961 cggatcaccc tgaatggcgc caccaagctc gccgatgacg gcggtcttac ctacacgggt
     1021 cccacgaact tcgagccggg cttcccgcag ctcgtccagc tcatcgagca gggcgacttc
     1081 gaggcgctga actcgtggta cgtcgggctc gatggcggcg actgcatccg cgccgcggtc
     1141 gtgaccggcc cgagccggat catcatcgat gtccagcact gaggtttgat atgacccact
     1201 ccccgcccat gggcgatctg gacctgtcaa agaactattc cgcgcggatc acgaccgagc
     1261 gcggcgcgtt cgacgttgag ctgttcgcgg ccgacgcccc gctcacggtc gagaacttcg
     1321 tgagcctggc ccgcgccggg ttctacgacg gcacgacctt ccaccgggtc atccccggct
     1381 tcatggccca gggcggtgac cctacgggta cgggcagcgg cggccccggt taccagttcc
     1441 gtgacgagtt cggctcgtca cgcagccacg acgcggccgg cgtcctgtcc atggccaacg
     1501 caggcccgaa cacgaacggc agccagttct tcatcacctt tggtccgacg ccgcacctcg
     1561 acgggaagca caccgtcttc ggccgggtga ccgacggttt ggacgtggcg atgggcatcc
     1621 gcgtgcgcga ccccggccgt gatccgaatc cgggcgaacg catcgagcgg atcgagatca
     1681 ccgaga
//
LOCUS       DASYGU010000210        15304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_702734, whole genome shotgun sequence.
ACCESSION   DASYGU010000210 DASYGU010000000
VERSION     DASYGU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702734"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VGO32_05825"
     CDS             complement(<1..446)
                     /locus_tag="VGO32_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7200305.1"
                     /translation="MLLEGKKVLVTGVLTDDSIAFSVAKVAQEAGAEIVLTSFGRATR
                     LTERIARRLDPVPDVLEMDVNQDDQIAAVAADLEHRWGRLDGLLHAIGFAPQDAMGGD
                     FLATPWESVATAFQTSAYSLKALTAGMRPLLKVSGADGGASVVTLT"
     gene            492..947
                     /locus_tag="VGO32_05830"
     CDS             492..947
                     /locus_tag="VGO32_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200306.1"
                     /translation="MMVRLYDYLVPVADAAVGVPNMREALADLSTDERKTLLTSLFKP
                     ERMPGSMERLTTLVLDVDASFGDLLTVLDNGTRERRFVAIDEARPILDRYLPDAAVEG
                     TREWDVARLHEPYGDGTYHGKLLKRVRKQLRSVGTREALTTPFLFRREW"
     gene            952..1083
                     /locus_tag="VGO32_05835"
     CDS             952..1083
                     /locus_tag="VGO32_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200307.1"
                     /translation="MTRKRRGRPYQPLPSRTNRTYAIVIAIIGIVFVVGFGVLASVR"
     gene            complement(1093..2271)
                     /locus_tag="VGO32_05840"
     CDS             complement(1093..2271)
                     /locus_tag="VGO32_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200308.1"
                     /translation="MTETDPPPRRRRRWPWLLAALAVVLIALIALPPARITLQTAALI
                     PSLLDLGPQPLALAPEPAHQMTTYEAPDGARLPADLWLPATASADHPVGAVIFVSGIN
                     SQGRSHPALIRVAKAIARSGAGVYIPELPFFFQAEVDASEVGRIVAAFQDLAEHPEVD
                     PSRIGIMGISVGGSLALIAAADPLIADRLAWVGAFGAYADAAEIMTDVISHQYRIDGK
                     IVDWHPTLLVRQVVFGLVTNQVSNGRDHGYLYGAYDSLNNEDVHPLPDAHIPLRTEAA
                     RAAEAILLTDTLPEAEAALAAAPPDLTAILDGISPIRHVAGIEARVYLMHDVGDQHIP
                     YAHAHELLAAMEEAGVDVRLGEFRLFDHVQPQTQDLAAAAPELWKLFWYVRGMAAEAL
                     "
     gene            complement(2268..2711)
                     /locus_tag="VGO32_05845"
     CDS             complement(2268..2711)
                     /locus_tag="VGO32_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200309.1"
                     /translation="MLQPMGVGHVVLKVRDLDRSLAFYRDLLGFQVSGELSNVMVFLT
                     ATGANHHDLGLVRVGDQAPSPVPMAVGLYHVAIQLADLDAIKEAHAVLSDRGLLRGAS
                     DHGVSKSLYTVDPDGNEIELYCDAPRDEWEGRVAEVMTVKPLHLD"
     gene            complement(2722..3345)
                     /locus_tag="VGO32_05850"
     CDS             complement(2722..3345)
                     /locus_tag="VGO32_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200310.1"
                     /translation="MPESIRRGAVHVLVIALIVAALAPQAVAATPSDPTTERLLAAVE
                     ASPPSLAASRGSTTQRSLATFTVAQPATAVGTERFQAGGLATPPSVVMEATVEGPYVQ
                     SVRQPGGAGTITGTASWYCCTMNWGNAAVVALPVALGGHYYAPPGGGTVVVCADRCAR
                     LPIGDACGCHWGWAGQKVVDLSPAAWAAISDKDRYVYGVLTVTVYLD"
     gene            3514..4296
                     /locus_tag="VGO32_05855"
     CDS             3514..4296
                     /locus_tag="VGO32_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV7200311.1"
                     /translation="MATDDKPRRSGPVVTRGPVNRPTEDQKLLQRRASAEFQATDTWR
                     SLRILGEFVEGFDALANVGPAVSVFGSARVRRSHPQYRLARRLAARLVGEGFAIITGG
                     GPGIMEAANRGARDAGGLSIGCNIELPFEQDVNPYVDLRIEFRYFFVRKTMFVKYAEA
                     FVIFPGGMGTMDELFEALTLIQTGKIEHFPVVLVGRDYWKGLLSWMRTTVLQEGKVSP
                     DDLELAVVTDDLDEVVRVIVERSQGRQQLVEGVAGDGLIRGG"
     gene            complement(4324..4400)
                     /locus_tag="VGO32_05860"
     tRNA            complement(4324..4400)
                     /locus_tag="VGO32_05860"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4364..4366),aa:Asp,seq:gtc)
     gene            complement(4459..4995)
                     /locus_tag="VGO32_05865"
     CDS             complement(4459..4995)
                     /locus_tag="VGO32_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200312.1"
                     /translation="MGIDAQPRRLIRASRLLFVLSGLLIGAGLVCIVGGSIGGAEIAD
                     RLLARLLAEVDPDTLAALPPDFLTAETVVRAATALGVGLLLLGVAQLATSIGLRRRQR
                     WSYAAAVVGGLFVAITAGATAVFMIVATAAQPQATIPLAVGAMVLAGVSILYAAIAIW
                     TASGRRTLEQSMAEAATI"
     gene            complement(5001..5471)
                     /locus_tag="VGO32_05870"
     CDS             complement(5001..5471)
                     /locus_tag="VGO32_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200313.1"
                     /translation="MTTSADRQPGGRPHSLAPEHLGDVWAGAALAIVVLGVAIFIVAI
                     GMVVSGLTATSSLDPANLPPNADSLGTTQLLVGIALFVVGVALAGGGLSLLGGSRRAR
                     KPVATVAIIAAIGAVVGGVVIVLRGPSDLVLVLTLIGVGLGLGSAGLLLLRPRP"
     gene            5552..6586
                     /locus_tag="VGO32_05875"
     CDS             5552..6586
                     /locus_tag="VGO32_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7200314.1"
                     /translation="MAFQMPVDPREARRARNVGWATTILSGLLIGVIAYLAYAGFQGS
                     DQLVHPGASRNCLLPSAMAWAYEAINYDQASDAALAAEPDPANCTANGAPAGPTLVTA
                     DGIRIAGWYIPAAAPIGPTGPTVVLIHGHASNKSGMLPEAAVLHEMYNLVLFDLRNHG
                     QSSGSETTGGITERADLEAVVAWLNATHAPTAIGLLGSSLGGIIATRAVAAGLPVQAL
                     ILDSTPASVADATRMHIEAMGLPLALPASWAVMLGTLFRTGIDVTAADPAITIDDVGR
                     VPVLIIQGSADRAIAADSATIIARAATDGGVTVESDICDGGEHSRLTEACPDAYRTWV
                     LGFLARTLGP"
     gene            6603..8246
                     /locus_tag="VGO32_05880"
     CDS             6603..8246
                     /locus_tag="VGO32_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7200315.1"
                     /translation="MTTIARPDAAQTAEDTRFDEVIERWFRERLQIQPEWATYLGIHD
                     HDHRLSLGGRDGIDEDIAFTRAATAAMDTFDPGTLSPNRALDRELVQHEARLHLFELT
                     ERRSWEGRSDGAEAIGGALFPLFTRDFAPPAERMESIAARLEASPEYLEQTRERVERP
                     VRLWTEIDIESGEGLPSFLDTILEAARGQDVPSATVRRLEEAVAATNAALDIHQAWLR
                     DDVLPRSSHEWLAGTELFEEMVRLRALEASGDEILAVGEEMLAQAHEQRNAVCAEIDP
                     SASREAIADQVKNDYPPTFADALEEYRRSMGRARAFVVEHDLATLPPNDILVVIETPT
                     FIRHLVPFAAYFDPARFDANAQGTYIVTPPETPEMMREHNYASISNTSVHEAYPGHHH
                     QLAAAVTNPSLVRLFSTSPEFTEGWAFYTERMMKEAGFDNTPTHRYVQLTDVIWRATR
                     IVLDVRLHRGEIGFEAAVDRLVDETGFERPAALAEVKRYTSTPTYQLSYLYGRHMIEG
                     LRREVEAREGADFDLKRFHDTLLYGGTMPVSFARRLFTS"
     gene            8243..8953
                     /locus_tag="VGO32_05885"
     CDS             8243..8953
                     /locus_tag="VGO32_05885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7200316.1"
                     /translation="MIGPLRPQGHAARTGLAGLVLVLVLAACEGGPLGGPPPTECPTA
                     APTATEAAAILEDVTTATFDTSLGVFTITLNAAAAPIASANFVALARCDFYDGVTFHR
                     VLAGFVAQAGDPNTRDNQGDFEGLGTGGPGYTFTVEIPPEGLNYDPYTVAMGNRNTLD
                     SNGSQFFICLTDLDASFTDRIYTIFGLVTDGREVVDAIGQVEVISPITGVPVDPVVIR
                     NIELSGPVEPAESTPSAS"
     gene            9219..9294
                     /locus_tag="VGO32_05890"
     tRNA            9219..9294
                     /locus_tag="VGO32_05890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9252..9254,aa:Arg,seq:ccg)
     gene            9316..9738
                     /locus_tag="VGO32_05895"
     CDS             9316..9738
                     /locus_tag="VGO32_05895"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7200317.1"
                     /translation="MRFEQSTDIDARQQRVWDVLTDLEGWPQRIETVDFVELLTPAPV
                     GEGSHVRLKQPKLPEGTWEVTVWDAPSYFEFRQKSGGVTSVAGHRVDALEEGRSRLTL
                     TLEMQGLLVPIFGRLYRDLTNRYMTIEAQGMKHAAESG"
     gene            9788..10486
                     /locus_tag="VGO32_05900"
     CDS             9788..10486
                     /locus_tag="VGO32_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEV7200318.1"
                     /translation="MTTESVTALLLIVVPLAFNAAFFELGRSFDYPSILRREPDEILR
                     RFAAGGSGLILRWEVLLISAMMMLPLAALLAIVLAAPPALSVLSLVVGSAAALVQSLG
                     LVRWPFAVPELARRYLAAPDGPDGDATRRTVEVVFGTLHRLLGVGIGEHLGYLLTGLW
                     TLLVSASIWSTAVLPGWLGVIGVVIGVALLIGTLEFVGPNERDGWHLAGVIVPVAYLS
                     WSVWLIVLGAFLLV"
     gene            complement(10499..12685)
                     /locus_tag="VGO32_05905"
     CDS             complement(10499..12685)
                     /locus_tag="VGO32_05905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV7200319.1"
                     /translation="MLTLKLVSVRAAAAGLVMALLATGLAPGVPVAAPKPAAIRTQAS
                     ETVHAVDPAGHDFALPAGTTHVAVHWPGHPDAVVEAAFSSDGEHFTPAALVEVDEVGA
                     ARGDGETYGSVMTAADKRVVRVTADRPLDGVAVLAMDAGAEHAAPPLGLGAQASALTS
                     VPGVIPRSAWGADETIRFDSHGEVRWGLAYFPLQKLIVHHTAGSNYDPNPAATVRAIY
                     HYHAVTQDWGDIGYNYLIDSAGRVYEGRWSREYWSGETPTSDNGNGLTVAGGHALQHN
                     SGTMGIALLGTFDGVAPTSAARASLVGLLAWAASVNGINPTGSSTYVNPVTGLTRYVP
                     NIAGHRSYNSTGCPGTTLNAMLPSIRSSVASVMNAWAGELYNPRRTLSLASGSHVGYR
                     FNPAGGITASKTYTLAASSSAPTSQRATVPGRSGSWYLVTAGVWAGYWIQESARTTLG
                     PAPPDQVGESYLPFRMLSFSAGTYVGRRFNSYGTVIGSKSYTLGADSMATTTEKSTIP
                     HQAGNWYYITTGVWDGYWIQESTGTVLGEPPPPPPPDEVQDYDPPAVLTFAPGTYVGR
                     QFNAFGQIVASKPYTLAAASSAPTDTRSTVYSQSGDWYHIVAGVWDGYWIQEGPGITL
                     ADPIPLAPDPPPDPEPPPVQPTVIEAYDPPQTLTFAAGTYVGRQFDADGAITTSRPYT
                     LTTSSAAPTNQVSEIPNQPGAWYFITAGIWAGYWIAESPDIELGGP"
     gene            12806..13354
                     /locus_tag="VGO32_05910"
     CDS             12806..13354
                     /locus_tag="VGO32_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200320.1"
                     /translation="MFVRGCLVLGVLLIVAACAGATPSTTPSGTALTWAEGDSLHLGV
                     VFTMPFSREPYDEPPFSDAVLIQENVSYVGPEGTNVGQGVLTSIEYKVPGLALMCAED
                     AFENQLGHDLTDPPGELTGELAGGITFLAIPERQVVAYGEDGLPTCWEHWGTYTATVP
                     DGSGTSERTGEYHAVLGTLDLE"
     gene            13351..14376
                     /locus_tag="VGO32_05915"
     CDS             13351..14376
                     /locus_tag="VGO32_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV7200321.1"
                     /translation="MSESGRQAPLFRRDRPVREAEEERLSAIATRSTASRGRLRAEEP
                     DPFRTAFERDRDRIIHSKAFRRLKHKTQVFLDPEGDHYVTRLTHTLQVAQIARSLAAA
                     LALNEPLAEAIALGHDVGHTPFGHTGEEALSPYFPPDGWHHAAQSVRVYEVLEDLNLS
                     WEVRDGIRAHTWKITPPPSTAEALCIRYADRIAYLTHDALDAVRAGVLAEADFPPHLL
                     ERLGPPGRQWIGELITAVIEESLRSGAVAMDAETLGLMHELRDFMFARVYLRPEQAAQ
                     QQVAVDLLRRLMDHHLVHPNEIPATYRDTDAPLVTQVSDYVSGMTDRFAQAASDRLFG
                     PEPSRSG"
     gene            complement(14368..14730)
                     /locus_tag="VGO32_05920"
     CDS             complement(14368..14730)
                     /locus_tag="VGO32_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200322.1"
                     /translation="MNWRILGCGLLAAAVFIGLGLLALNLAIGRAGCPPTLQWGDRLY
                     DAVGDPTDEPVVGDGEPVLLGLTVAGALTREVYGPEGSTPSADPGHQPDQIALACGDA
                     TFQTYVFSAVLHTAEPSP"
     gene            complement(14727..15032)
                     /locus_tag="VGO32_05925"
     CDS             complement(14727..15032)
                     /locus_tag="VGO32_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV7200323.1"
                     /translation="MARARATVTSGQRLTARVIGRVQGVGYRWWVVQRASALGLVGWV
                     RNADDERSVELVAEGDASALDALEAELTGGPGGARVDAVEASRGPASGGWVRFEIRR"
     gene            complement(15008..>15304)
                     /locus_tag="VGO32_05930"
     CDS             complement(15008..>15304)
                     /locus_tag="VGO32_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200324.1"
                     /translation="ETATRTPTAVQRAAALAITDAWVDLARDLAVAAAGAPRLAASSE
                     LVPDLATAARGRDAQGWADAALAIERIRDALQENVSPRLALETAMLAWPELAPR"
BASE COUNT         2397 a         5285 c         5307 g         2315 t
ORIGIN      
        1 aaggtcaggg tgacgacgga tgccccgcca tccgcgcccg ataccttgag caacgggcgc
       61 atacccgcgg tcagcgcctt cagcgagtac gcgctcgtct ggaacgcggt cgcgacgctt
      121 tcccacgggg tggccaggaa gtcgccgccc atggcgtcct gcggggcgaa gccgatggca
      181 tgcagcagcc cgtcgagccg gccccagcgg tgctcgaggt cggcggccac cgccgcgatc
      241 tggtcgtcct ggttgacgtc catctcgagc acgtcgggga ccgggtcaag ccggcgggca
      301 atccgctcgg tgagcctcgt ggctcgcccg aagctggtca ggacgatctc ggcaccggcc
      361 tcctgggcga ccttggccac cgagaacgcg atcgagtcat cggtcaggac gccggtgacg
      421 aggaccttct tgccttcaag cagcatcgcc ggattgtacc ggccgtcgga acggctaccc
      481 tgcagggacc gatgatggtc cgcctgtacg actacctggt cccggtggcc gacgccgcgg
      541 ttggtgtccc gaacatgcgc gaggcgctcg ccgatctgtc gaccgatgag cggaagacgc
      601 tcctgaccag cctgttcaag cccgagcgga tgccgggctc gatggaacgg ctgaccacgt
      661 tggtcctgga cgtggatgcc agctttggcg acctgctgac tgtcctcgac aacgggaccc
      721 gggagcgacg gttcgtggcc atcgacgaag cgcgcccgat cctggaccga tacctgcccg
      781 atgcagccgt cgagggcacc cgcgagtggg acgttgcccg cctccacgag ccctacggcg
      841 acggcacgta ccacggcaag ctgctgaagc gtgtccggaa gcagctccgc tcggtgggca
      901 cccgggaggc gctgacgacc ccgttcctgt tccgacgcga atggtgagcg catgactcgg
      961 aagcgccgcg gccggcccta ccagcccctg cccagtcgga cgaaccggac ctacgcgatc
     1021 gtcatcgcga tcatcgggat cgtgttcgtg gtcgggttcg gggtgctcgc ctcggtccgc
     1081 taggggaatc ggtcagaggg cctcggcggc catgccgcgg acgtaccaga acagcttcca
     1141 cagctccggt gcggccgccg ccaggtcctg ggtctgcggc tggacgtgat cgaacagccg
     1201 gaactcgccg agccggacgt ccaccccggc ctcctccatg gcggccagca gctcgtgggc
     1261 gtgggcgtac gggatgtgct ggtcgccgac gtcgtgcatg aggtacaccc gggcctcgat
     1321 gccggccacg tggcggatcg ggctgatgcc atccaggatc gcggtcaggt ccggcggggc
     1381 cgcggcgagt gcggcttcgg cctcgggcag ggtgtcggtc agcaggatcg cctccgccgc
     1441 gcgtgccgcc tcggtccgca gagggatgtg agcatcgggc agtgggtgga catcctcgtt
     1501 attcaggctg tcatacgcgc cgtagaggta gccatggtcg cgcccgttgc tcacctggtt
     1561 ggtgaccagg ccgaagacga cctggcggac gaggagggtg gggtgccagt ccacgatctt
     1621 cccgtcgatc cggtactggt ggctgatgac gtcggtcatg atctcggccg catcggcata
     1681 ggccccgaat gcgccgaccc aggccagccg gtcggcgatc agcggatcgg ccgcggcgat
     1741 gagcgccagg ctgccgccga cggagatgcc catgatcccg atccgactgg ggtccacctc
     1801 cgggtgctcg gccaggtcct ggaacgcggc cacgattcgg ccgacctcgc tcgcgtccac
     1861 ctccgcctgg aagaagaacg ggagctcggg gatgtagacc ccggcgccac tgcgggcaat
     1921 ggcctttgcg acgcggatca gggccggatg ggaccgccct tgtgagttga tccccgacac
     1981 gaagatgacc gccccgaccg ggtggtcggc cgaggcagtg gccggcagcc acaggtcggc
     2041 tggcaggcgt gcgccatccg gggcctcgta ggtcgtcatc tggtgcgcgg gctcaggtgc
     2101 gagcgcgagt ggctgggggc cgagatcgag cagggagggg atgagcgccg cggtctggag
     2161 cgtgatcctc gccggcggca gggcgatgag cgcaatgagg accaccgcca gcgcggccag
     2221 cagccaaggc cagcgacgac gacgtcgcgg gggtggatcg gtctcggtca atccaggtgg
     2281 agcggtttga ccgtcatgac ctcggcgacc cgaccttccc attcgtcgcg gggtgcgtcg
     2341 cagtagagct cgatctcgtt gccgtcagga tccacggtgt acaggctctt gctcacgcca
     2401 tggtcggagg cgccgcgcag aagcccgcgg tcggagagca cggcgtgcgc ctccttgatc
     2461 gcgtcgagat cggccagctg gatggcgacg tgatacagac caaccgccat gggcaccggt
     2521 gatggcgcct ggtcgccgac ccggacgagg ccgaggtcat ggtggttcgc gccggtcgcg
     2581 gtgaggaaga ccatcacgtt gctcagctcg cccgagacct ggaacccgag caggtcgcgg
     2641 tagaaggcga gtgatcggtc caggtcacgc actttgagca cgacatggcc gacccccatc
     2701 ggctgcagca cgcgatcgat gctagtccag atacacggtg acggtcagaa cgccgtagac
     2761 gtaccggtcc ttgtcgctga tggcagccca cgccgccggc gacaggtcga cgaccttctg
     2821 gccggcccaa ccccagtggc atccgcacgc gtcgccgatg ggcaggcgag cgcagcggtc
     2881 ggcgcaaacg acgacagtcc cgccgcccgg tggcgcgtaa tagtggccgc cgagggcaac
     2941 gggaagcgcg acgaccgccg cgtttcccca gttcatcgtg cagcagtacc agctcgcggt
     3001 gccggtgatc gtccctgccc cgccgggctg ccgaacggac tggacgtacg ggccttcgac
     3061 cgtggcctcc atgaccacgg agggcggtgt ggccagccct ccggcctgga aacgctcggt
     3121 gcccaccgcg gtcgcgggct gggcgacggt gaacgtggcg aggcttcgct gggtggtgct
     3181 cccgcgcgac gcggcgaggg acggaggaga cgcctcgacc gcggccagca ggcgctcagt
     3241 ggttgggtcc gagggagtcg cagccaccgc ctgcggcgca agggcggcaa cgatcagggc
     3301 aatgaccagg acgtggacgg caccacgtcg aattgactcg ggcaaagagt atcctcgggc
     3361 acagatttga cgggatccgg ctcgccgcgg ccggctcaca tcgggcaccg ggcaggcgcc
     3421 gcgcgacgac gacgtttccg gggaccctac catgttcatc ctggtcccgc ccgttcggac
     3481 ggccggacga tcagagcgca aaagaggtga ccaatggcga ccgatgacaa gccgcgccgg
     3541 agcgggccgg tcgtcacccg cgggccagta aaccgtccga ccgaggacca gaagctcctc
     3601 cagcgacggg cgagcgccga gttccaggcc accgacacct ggcggtcgct ccgcatcctt
     3661 ggcgagttcg tcgagggctt cgacgccctg gccaatgtcg gtccggcggt cagcgtcttc
     3721 ggctcggccc gcgtccgtcg gagccacccc cagtaccgcc tcgcccgacg cctcgccgcc
     3781 cggctggtgg gcgagggatt cgcgatcatc acgggcggtg ggccggggat catggaggcc
     3841 gccaaccgcg gcgcgcgcga cgcgggtggg ctgtccatcg gctgcaacat cgagcttccg
     3901 ttcgagcagg acgtcaaccc gtacgtcgac ctccggatcg agttccgcta cttcttcgtg
     3961 cgcaagacga tgttcgtgaa atacgccgag gcgttcgtca tctttcccgg cgggatgggg
     4021 acgatggacg agttgttcga ggccctgact ctgatccaga ccggcaagat cgagcacttc
     4081 ccggtcgtcc tcgtgggccg cgactactgg aagggcctgc tgtcgtggat gcggacgacc
     4141 gtgctccagg agggcaaggt gagccccgac gacctggagc ttgcggtcgt gaccgatgac
     4201 ctggacgagg tcgtgcgggt catcgtcgag cggagccagg gacgccagca gctcgttgag
     4261 ggagtagcgg gcgacggcct gatccgaggg ggctgagtgc cgctcgcggc tgacggggaa
     4321 tgctggcggg cccgaccgga ttcgaaccgg cgatctccag cgtgacaggc tggcatgttg
     4381 ggccgctaca ccacgggccc gcggcggcgg ggcggagtat accaagtgcg tcccgcggcc
     4441 ctcgaacggc gggccgggtc agatggtggc ggcctcggcc atggactgct cgagcgtacg
     4501 acggccactc gccgtccaga tcgcgatcgc cgcgtagagg atcgacaccc cggccagcac
     4561 catggcaccg accgccagcg ggatcgtcgc ctgcggctgg gccgcggtgg cgacgatcat
     4621 gaacaccgcg gtcgcgcccg cggtgatggc cacaaacagg cccccgacga ccgcggccgc
     4681 gtaggaccag cgttggcggc gacgcagacc gatggacgtc gcgagctgcg ccacccccag
     4741 gagcagcaac ccgaccccga gggccgtcgc ggcgcggacg acggtctcgg cggtgaggaa
     4801 gtccggcggc agcgcggcga gggtgtcggg gtcgacctcg gccaggagcc gggccaggag
     4861 ccggtcggca atctcggccc cgccgatgct gccgccgacg atgcagacca gcccggcgcc
     4921 gatcaatagc ccggacagga cgaacagcag tctcgaggcg cggatcaggc ggcggggctg
     4981 ggcgtcgatg cccacccggg tcagggccga ggtctgagca ggagcaggcc ggcgctgccg
     5041 agtccgagtc ccaccccgat gagtgtcagg accagaacga gatccgacgg accgcgaagc
     5101 acgatcacca ccccgccgac gacggcgccg atggccgcga ttatcgccac ggtcgcgacc
     5161 ggcttgcgag cgcggcggct cccgccgagc agcgagaggc caccacccgc gagcgcgacg
     5221 ccaacgacga acagcgcaat gcccaccagc agctgggtgg tgcccaggct gtcggcgttg
     5281 ggaggcaggt tggccgggtc aaggctcgag gtcgcggtca gacccgagac gaccattccg
     5341 atcgcgacga tgaagatcgc gacgcccagc acgacgatgg ccagcgcggc accggcccac
     5401 acatcgccga ggtgttccgg cgcgagcgaa tggggtcgac cgccgggctg gcgatcggca
     5461 ctggtggtca tgggtatcct ctgacgggcg gcgggcattg tagggtgggg cgacggccgg
     5521 cgtcaccacc cagcctatac tcggtcacgg catggcattt cagatgcccg tcgatccccg
     5581 ggaggcgcga cgggctcgga acgtcggatg ggccacgacg atcctgtccg gcttgctgat
     5641 cggcgtcatc gcctacctcg cctacgccgg gttccaggga tcggaccagc tcgtccatcc
     5701 cggggccagc cggaactgcc tgctgccctc cgcgatggcc tgggcctacg aagcgatcaa
     5761 ctatgaccag gcctcggatg cggccctggc cgccgaaccg gaccccgcca actgcaccgc
     5821 caacggcgct cctgccgggc cgacgctggt gaccgccgac gggatccgga tcgcgggctg
     5881 gtacatcccg gccgccgctc ccatcggccc gaccggacca accgtcgtcc tcatccacgg
     5941 ccatgccagc aacaagagtg gcatgctgcc ggaggccgcg gtcctgcacg agatgtacaa
     6001 cctcgtgctg ttcgacctca ggaaccacgg gcagagctcg ggctccgaga cgaccggcgg
     6061 gatcactgaa cgggccgacc tcgaggctgt cgtggcctgg cttaacgcca cccacgcgcc
     6121 aacggccatc ggtctgctgg gcagctcgct gggcgggatc atcgcgaccc gcgcggtcgc
     6181 cgccggtctg ccggtgcagg cgctcatcct cgactccact ccggccagcg tcgccgacgc
     6241 gacccgaatg cacatcgagg ccatgggcct tccgctcgcg ctgcccgcat cgtgggcggt
     6301 gatgctcggc acactgttcc ggaccgggat cgacgtcacc gccgcggacc cggcgatcac
     6361 gattgacgat gtcggccgcg tgccggtgct catcatccag ggctcagccg accgggcgat
     6421 cgcggccgac tcggcgacca tcatcgcccg ggcggcgact gacggtggcg tgactgtcga
     6481 gtccgatatc tgcgacggcg gagagcattc ccgtctgacc gaggcgtgcc cggatgcata
     6541 tcggacgtgg gtgctaggct tcctcgcccg cacgctcggc ccatgaccct cggaggagct
     6601 tcatgaccac gatcgcccgg ccggatgccg cccagaccgc cgaagacact cgattcgacg
     6661 aggtcatcga gcgatggttc cgcgagcgtc tccagatcca gcccgagtgg gccacgtacc
     6721 tcggcatcca cgaccacgac catcgcctgt cactcggcgg gcgggacggg atcgacgagg
     6781 acatcgcctt cacccgcgcg gccacggcgg ccatggacac cttcgatccc ggcaccctct
     6841 ccccgaaccg ggcgttggac cgcgagctcg tccagcatga agcccggctc cacctgttcg
     6901 agctgaccga gcgtcgatcg tgggaggggc gctccgacgg agccgaggcg atcggcggcg
     6961 cgctgttccc gctgttcacc cgcgacttcg cgccgcccgc cgagcggatg gagagcatcg
     7021 ccgcccgcct ggaggcgtcg ccggagtacc tcgagcagac ccgcgagcgg gtcgaacggc
     7081 cggtgcggct gtggaccgag atcgacatcg agtcggggga gggtctcccg tcattcctgg
     7141 acacgatcct cgaggccgca cgcggccagg acgtgccgtc cgccaccgtt cggcggctgg
     7201 aggaggccgt agccgccacc aatgccgcgc tcgacatcca ccaggcgtgg ctccgcgacg
     7261 acgtcctgcc ccgttcctcg cacgagtggc tggccggcac cgagttgttc gaggagatgg
     7321 tccgcctccg tgcgctggag gcgagcggcg acgagatcct ggccgtgggc gaggagatgc
     7381 tggcccaggc ccacgagcag cgaaacgcgg tctgcgccga gatcgatccc tccgcctcgc
     7441 gtgaggcgat cgcggaccag gtcaagaacg actacccgcc cacctttgcc gacgcgctcg
     7501 aggagtaccg gcggtcgatg ggccgcgccc gcgcgttcgt cgtggagcac gacctggcca
     7561 cgctgccccc gaacgacatc ctggtcgtca tcgagacgcc gaccttcatc cgccatctcg
     7621 tgccattcgc ggcctacttc gatccggcgc gcttcgacgc gaacgcccag ggcacgtaca
     7681 tcgtcacgcc gcccgagacg cccgagatga tgcgcgagca caactacgca tccatctcca
     7741 acacctcggt ccacgaggcg taccccggcc accaccacca gctcgcggcg gccgtgacaa
     7801 acccgagcct cgtccgcctg ttctcgacgt cacccgagtt caccgagggc tgggcgttct
     7861 acaccgagcg gatgatgaaa gaggccggct tcgacaacac gcccacccat cggtatgtcc
     7921 agctgaccga tgtcatctgg cgcgcgaccc ggatcgtcct cgacgtccgg ctgcatcgcg
     7981 gagagatcgg attcgaggcc gccgtcgatc ggctcgtgga cgagaccggc ttcgagcgtc
     8041 cggcggcgct ggcggaggtc aagcgctaca cgagcacgcc gacctaccag ctgtcctatc
     8101 tatacggccg acacatgatc gagggcctcc gccgcgaggt ggaggcgcgt gaaggcgcgg
     8161 acttcgacct caagcggttc cacgacaccc tcctgtacgg cggcacgatg cccgtctcgt
     8221 tcgcccggcg tctgttcacc tcttgatcgg accgctgcgc ccccaagggc acgccgcacg
     8281 gaccggcctg gccggcctcg tcctcgtcct cgtgctcgcc gcgtgcgagg gcggaccgct
     8341 cggtggccca ccgcccaccg agtgcccgac ggccgcgccc acggcgaccg aggccgcagc
     8401 catcctcgag gacgtgacga ccgcgacctt cgataccagc ctgggggtgt tcacgatcac
     8461 gctgaacgcc gccgcggcgc cgatcgcgtc cgcgaacttc gtggcccttg cccggtgcga
     8521 cttctacgac ggcgtgacct tccatcgggt cctggccggc ttcgtcgccc aggccggcga
     8581 cccgaacacc cgcgacaacc agggcgactt cgagggcctg ggcacgggcg ggccgggata
     8641 tacgttcact gtcgagatcc cgcccgaagg actgaactac gacccgtaca cggtggcgat
     8701 ggggaaccgc aacacgctcg actcgaatgg gagccagttc ttcatctgcc tgaccgacct
     8761 ggacgccagc ttcaccgacc ggatctacac gatcttcggg ctggtcaccg atgggcggga
     8821 ggtggtcgat gcgatcggac aggtggaggt catcagcccg atcaccggcg tcccggttga
     8881 cccggtcgtc atccgcaaca tcgagctgag cggaccggtc gagccggcgg aaagtacgcc
     8941 ctcggccagc tgagcccggg tacgaaaatc cgctccgaaa agtaaaggat tcttcattcc
     9001 aaagcattga cccgatcccg ggggtcggat atcatcttgt cgctgttcgc acagcgattc
     9061 accactggtc gcagggtcat gggtaggttt gggtaagggt agtgcgggaa accgcgcgtg
     9121 acagatgccc ccgcgattag cgaatccggc agcaatgaag gcccctcttc ggaggggcct
     9181 tcgcttatgc ccgggggacg ggctacactc ggccacccgc gccggtagct cagtggatag
     9241 agcgttggcc tccggagcca aaggtcgcag gttcgaatcc tgtccggcgc gccagattcg
     9301 aggttgccgt ccgccatgcg ctttgagcaa tccaccgata tcgacgcccg gcagcagcgt
     9361 gtgtgggacg tgctcaccga cctcgaaggg tggccccagc ggatcgagac ggtcgacttc
     9421 gtcgagctgc tcacgcccgc tccggtgggt gaaggcagcc acgtccgcct caagcaaccg
     9481 aagctgcccg agggcacctg ggaggtcacc gtctgggacg ccccgtccta tttcgagttc
     9541 cgccagaagt cgggcggcgt cacgagcgtc gcaggccatc gtgtcgatgc gctggaggaa
     9601 ggtcgctccc gcctgaccct gacactggag atgcaaggcc tgctggtccc gatcttcggt
     9661 cggctctaca gggacctcac caaccgatac atgaccatcg aggcgcaggg catgaagcac
     9721 gccgccgagt ccgggtgaga cgagccgcgc ttcgtgcggc gcttcgtgcc gcgctcgtga
     9781 tcgccgcgtg acgaccgagt cagtcaccgc gctgctgttg atcgtggtgc cgctcgcctt
     9841 caacgcggcg ttcttcgagc tcggacggtc cttcgactat ccgagcatcc tccgcaggga
     9901 gcctgacgag atcctccggc gcttcgcggc cggcgggtcg gggctcatcc tgcgctggga
     9961 ggtcctgttg atctcagcga tgatgatgct cccgctggcg gcgctgctgg ccatcgtcct
    10021 ggccgctccg ccggccctga gcgtcctgtc gctcgtcgtg ggttcggccg cggccctcgt
    10081 ccagagcctc ggactggtgc gatggccgtt cgccgtgcca gagctcgcgc gacgctattt
    10141 ggcggcaccc gacggccccg acggcgatgc gacccgtcgg accgtggaag tcgtattcgg
    10201 aaccctccac cggctccttg gggtcgggat cggcgaacac ctgggatacc tgctgacggg
    10261 tctgtggacg ctcctggtct ccgcgtcgat ctggtcgacc gcggtcctgc cgggctggct
    10321 cggcgtcatc ggcgtcgtca tcggggtcgc gctgctgatc ggcaccctcg agttcgtcgg
    10381 acccaacgag cgcgacggct ggcacctggc cggggtgatc gtgccggtcg cctacctgtc
    10441 gtggtccgtg tggctcatcg tgctcggcgc attccttctc gtctgagggc gactgctcct
    10501 acggcccgcc cagctcgata tcgggcgact ccgcgatcca gtacccggcc cagattccgg
    10561 cggtgatgaa gtaccacgct cccggctggt tcgggatctc gctgacctgg ttcgttggcg
    10621 ccgctgacga agtggttagg gtgtacgggc gggacgtcgt gatcgcaccg tccgcgtcga
    10681 attgccgccc gacgtacgtg cccgcggcga acgtgagggt ctgcggcgga tcgtaggctt
    10741 cgatcacggt cggctggacc ggcggcggct cggggtccgg cggcggatcc ggggccagcg
    10801 ggatcgggtc ggcgagggtg atccctggac cttcctggat ccagtagccg tcccagacgc
    10861 cggcgacgat gtggtaccag tcccccgact ggctgtagac cgtgctccgc gtgtcggtcg
    10921 gggccgagga agctgccgcg agggtgtatg gcttcgaggc cacgatctgc ccgaacgcat
    10981 tgaactggcg accgacgtac gtacccgggg caaaggtcag gaccgccggc gggtcgtagt
    11041 cctggacttc atccggcggc gggggcggtg gaggctcacc caggaccgtg cccgtcgact
    11101 cctggatcca gtagccgtcc cataccccgg tcgtgatgta gtaccagttg cccgcctggt
    11161 gcgggatcgt gctcttctcg gtcgtggtgg ccatcgagtc cgcgccaagg gtgtacgact
    11221 tcgagccgat caccgtccca tagctgttga acctgcggcc gacatatgtc cctgccgaga
    11281 agctcagcat ccggaacggg aggtacgact cgcccacctg gtcgggcggc gccggaccca
    11341 gcgttgtgcg cgccgactct tggatccaat aacccgccca cacgccggcg gtcacgaggt
    11401 accaggaccc gctccggccg gggaccgtgg ccctctggct ggtcggcgcc gacgatgatg
    11461 cggcgagggt gtaggtcttc gacgcggtga tcccaccggc cgggttgaac cgatagccca
    11521 catggctgcc gcttgccagt gacagggtcc gccgcgggtt gtacagctcg cccgcccagg
    11581 cgttcatgac cgatgcgacc gagctccgga tcgacggcag catcgcattg agggtagttc
    11641 ccgggcagcc cgtcgagttg tagctgcgat gacccgcgat gttgggtacg taccgcgtca
    11701 agccggtgac cgggttgacg tacgtcgagc tgccggtggg gttgatcccg ttcaccgagg
    11761 cggcccacgc gaggagcccg acgagggagg cgcgggccgc cgacgtgggg gcgacgccgt
    11821 cgaaggttcc gagcagcgcg atgcccatgg tccccgagtt gtgctgcagg gcatggccac
    11881 cggccacggt gaggccattg ccgttgtcgg atgtcggggt ctccccgctc cagtactcgc
    11941 gcgaccagcg accctcgtag acgcgtcccg ccgaatcgat gaggtagttg tagccaatgt
    12001 caccccagtc ctgagtgacc gcgtggtagt gatagatcgc gcgcaccgtg gcggcggggt
    12061 tcgggtcgta gttcgaccct gccgtgtggt ggacgatcag cttctgaagc gggaagtagg
    12121 cgagacccca gcggacctcg ccgtggctgt cgaaccggat cgtctcgtcg gctccccagg
    12181 cgctgcgcgg gatcacgcca ggaaccgagg tcaacgccga tgcctgggca ccaaggccga
    12241 gcggcggggc ggcatgttcc gccccggcat ccatggccag gacggcgacc ccgtccagcg
    12301 gtcggtcggc ggtgacccgg actacgcgct tgtctgcggc ggtcatcacc gatccatagg
    12361 tctccccgtc gccgcgggcc gcgccgacct cgtccacttc gacaagggcc gcgggcgtga
    12421 agtgctcacc atcgctgctg aacgccgcct cgaccaccgc gtcggggtgc cccggccagt
    12481 ggaccgcgac atgggtcgtg ccggcgggaa gcgcgaagtc gtggcccgcg ggatccactg
    12541 cgtgcacggt ctcggacgcc tgggtcctga tggccgccgg cttcggtgcg gcgacgggca
    12601 caccgggggc gagcccggtt gcgagcaggg ccatcaccag accggcggcg gcggcacgaa
    12661 cggaaaccag cttcagcgtg agcattggag tcaccccgca gacccggcga gtatcgagac
    12721 caccctagaa cgctcgtgtc ccgctggctc tggccctgtc tgcccagtac gatcggcctc
    12781 tcagccgtgt gccagactgg ccgcgatgtt cgtccgcggc tgcctggtcc tcggcgtgct
    12841 gctgatcgtc gcggcgtgtg caggcgcgac gccgtcgaca accccttccg gcacggcact
    12901 cacgtgggcg gagggcgact cgctgcacct tggggtcgtg ttcacgatgc cgttcagccg
    12961 cgaaccctat gacgagcccc cattcagcga tgccgtcctc atccaggaga acgtttcgta
    13021 cgtgggtccg gaaggcacga acgtgggcca gggtgtgctg acctcaatcg agtacaaggt
    13081 cccaggcttg gcgctgatgt gcgctgaaga cgcatttgaa aaccagctgg gccatgacct
    13141 gacggatcca cccggtgagc tgaccggaga gctggcgggc gggatcacat tcttggcgat
    13201 ccccgagcga caggtggtgg cgtacggcga ggacggactg ccgacctgtt gggagcattg
    13261 ggggacgtac accgccaccg tccctgacgg cagcgggacg agtgagcgga ccggggaata
    13321 ccacgccgtg ctggggacgc tcgacctgga gtgagcgagt cgggacgcca ggcgccgctc
    13381 ttccggcgtg accgcccggt cagggaagcg gaagaggagc gtctgtcggc aattgcgacc
    13441 cgatcgacgg cctcgcgcgg ccggctgcga gccgaggagc cggatccgtt ccggaccgcg
    13501 ttcgagcgcg atcgggaccg gatcatccat tcgaaggcat tccggcggct caagcacaag
    13561 acccaggtct tcctcgaccc cgagggcgac cattacgtca cgcgcctgac ccacaccctc
    13621 caggtggcgc agatcgcacg gtcgttggcc gccgcgctcg ccctcaacga acccctggcc
    13681 gaggcgatcg ctctgggcca cgacgtcggg cacacgccgt ttggccatac cggagaagag
    13741 gcgctttccc cgtacttccc accggacggg tggcaccacg ccgctcagag cgtgcgcgtg
    13801 tacgaggtcc tcgaggacct gaacctgagc tgggaggtcc gggacgggat ccgcgcacac
    13861 acctggaaga tcacgccccc gcccagcacg gccgaagcgc tgtgcatccg ctatgcggac
    13921 cgaatcgcct acctcaccca cgacgcgctc gacgccgtgc gcgccggcgt cctggccgaa
    13981 gccgacttcc cgccccacct cctcgagcgg ctcggcccgc cgggcaggca atggatcggg
    14041 gagctcatca ccgccgtcat cgaggagagc ctgcggtcgg gcgcggtggc gatggacgcc
    14101 gagaccctcg gcctcatgca cgagctccgc gacttcatgt tcgcacgggt ctatctccga
    14161 ccggagcagg cggcacagca gcaggtggcc gtggatctgc ttcgtcgcct gatggaccac
    14221 cacctcgtgc atcccaacga gattccggcc acctaccgtg acaccgatgc ccccctcgtc
    14281 acccaggtct ccgactacgt gtccgggatg accgaccggt ttgcccaggc ggcctccgac
    14341 cgcttgttcg ggccggaacc gtcgcgatca gggtgagggt tcggcggtgt gaaggacggc
    14401 gctgaacacg taggtctgga acgtcgcatc accgcaggcc agcgcgatct ggtccggctg
    14461 gtggcccggg tccgctgacg gagtactgcc ttccggtccg tagacctccc gcgtgagcgc
    14521 gcccgcaacg gtcaagccca ggagcacggg ctcgccgtcc cccacgaccg gctcgtcggt
    14581 cgggtccccg accgcgtcat agagccggtc gccccactgg agagtgggcg gacagccggc
    14641 ccgaccgatg gccaggttga gcgcgagcag gcccagaccg atgaacaccg cggcggcgag
    14701 caggccacag ccgagaatgc gccagttcat cggcggatct cgaatcggac ccagccgccg
    14761 ctcgccggtc cgcgactggc ctcgaccgcg tccacccggg ccccgccagg ccctcccgtg
    14821 agctccgcct cgagcgcgtc gagggcgctg gcgtcgcctt cggcgaccag ctcgaccgag
    14881 cgttcgtcat cggcattccg gacccagcca acgagtccca gcgccgaggc ccgttggacg
    14941 acccaccacc ggtatccgac gccctgcacg cgaccgatca cccgggcggt caggcgttgg
    15001 cccgacgtca ccgtggcgcg agctcgggcc acgccagcat cgcggtctcc agcgcgaggc
    15061 gcgggctcac gttctcctgg agcgcgtccc ggatccgctc gatcgcgagg gccgcatcgg
    15121 cccagccctg cgcgtccctc ccccgcgcgg cagtggcgag atccggcacg agctccgagg
    15181 acgccgccag ccgaggcgcg ccggcggccg ccaccgccag gtcgcgggcc aggtcgaccc
    15241 acgcgtcggt gatcgcgagg gcggccgcgc gctgcacggc ggtcggggtt cgggtcgccg
    15301 tttc
//
LOCUS       DASYGU010000211         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1062836, whole genome shotgun sequence.
ACCESSION   DASYGU010000211 DASYGU010000000
VERSION     DASYGU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062836"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1017
                     /locus_tag="VGO32_05935"
     CDS             169..1017
                     /locus_tag="VGO32_05935"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEV7200325.1"
                     /translation="MTEALGRIGFAVSRYNPEAKAVLVRGRAWCENQGIATWDAVADD
                     EDKLASELPGSDLVVVLGGDGTFLRVARIIGDSEVPALGVNLGRVGFLAKVEPDELEP
                     ALDGIAAGRHRIAERMRLGATIHPRDGEPTTAACLNEVAVARAAQVRLIQVEVEVSGS
                     HLATYLADGVVVATPTGSTAYSYSAGGAVLDPRLRNLVITPVAAYLSALHSVVTGEDH
                     LIRLHLREAFGGAVVSFDGQREVAVEVGEWVEVRALPVPLRLVEPEGSTPFYDLLRTK
                     ASLLPD"
     gene            1036..1932
                     /locus_tag="VGO32_05940"
     CDS             1036..1932
                     /locus_tag="VGO32_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEV7200326.1"
                     /translation="MTTAAADPRLTAAIGEFLLELRVERGLSPLTIAAYRRDLDQFAA
                     GSGPGWRDDPAVVGEFVARLRRQGRRASTQARKVAAIRSFYGFARREGVIERDLADLI
                     DPPRPGRYLPEVLAPDEVERILAAPGDDPAGLRDAAILELLYACGLRVSELTGLDLDR
                     LNLGALEVRVIGKGNRERRVPMGEPARDRLHRYLSGPRQAWTAGKPTPAVFVSQRGTR
                     LVRESVWRLVRRWSQAAGVVGDITPHTFRHSFATHLLEGGADLRVVQSLLGHASISTT
                     QLYTHLTGERVREVYTRAHPRA"
     gene            1941..2735
                     /locus_tag="VGO32_05945"
     CDS             1941..2735
                     /locus_tag="VGO32_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV7200327.1"
                     /translation="MSYARKLLARGEDVVLESHQHWFVVIGRSWWAIVLAIFALAVLI
                     FVASPPEEGTDSAAEVVAFIFLLIAFARIGWVIWDWRNNEFLVTTRRIVRAEGILNKR
                     MVETSLEKVNDAVLTQSMFGRLFGFGDLDILTAADEMGGIEDFPMVADPVEFKVAMMN
                     QKEAVERPDIAPQAYQHQTAPELVPAEPMAPRAGSDRVTVVDEPAPAAQAPMEMPVDS
                     PPSSPAAAAPAEDATATLERLASLRDRGLITPEEYDAKKRELLERI"
     gene            2737..>3164
                     /locus_tag="VGO32_05950"
     CDS             2737..>3164
                     /locus_tag="VGO32_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV7200328.1"
                     /translation="MAADPASEDLGRRIETAAAAIRERLSAVPALATPRLGLVLGSGL
                     GAVADLLDPEPRIRIPYGEIPEVPVSGVQGHAGELIAGLVAGKPVLVLAGRVHAYEGY
                     SHREVTILIRATFRVGVEAVVLTNAAGGLDPDFEPGELML"
BASE COUNT          440 a         1106 c         1135 g          483 t
ORIGIN      
        1 cggcgtctca ccgtcatccc tgatgcgcgc gctcatcgcg gccgggctgg accgctcgta
       61 cgcggcgctc accgagtccg aggccgcctc cgatctggcg gccgagctcg ccgcgctccg
      121 tgagcggatc gaggacatcg ccaccagcct cgccgccgat cgcccctcgt gaccgaggcc
      181 ctcgggcgga tcggcttcgc ggtcagtcgt tacaacccgg aggccaaggc cgtcctggtg
      241 cgcggtcgcg cgtggtgcga gaaccaggga atcgcgacct gggacgccgt cgccgacgat
      301 gaggacaagc tcgcgtccga gctgccgggg agcgacctgg tcgtcgtcct gggcggggac
      361 ggcacgtttc tccgcgtcgc gaggatcatc ggcgactccg aggtgccggc cctcggtgtg
      421 aacctcgggc gggttggctt cctggccaag gtcgagcccg acgagctgga gccggcgctg
      481 gacgggatcg cggccggccg ccaccggatc gcggagcgga tgcgactcgg cgcgacgatt
      541 cacccccgag acggcgagcc gaccacggcg gcctgcctga acgaggtggc cgtggcccgc
      601 gccgcgcagg tgcggctcat ccaggtcgag gtcgaggtga gcggcagcca tctggcgacc
      661 tacctcgccg acggcgtcgt cgtcgccacg ccgaccgggt cgaccgcgta ctcgtattcg
      721 gccggcgggg cggtcctcga tccgcggctg cggaacctcg tgatcacgcc ggtggccgcc
      781 tacctgtccg cccttcacag cgtggtgacc ggcgaggatc acctcattcg actccacctc
      841 cgtgaggcgt tcggcggggc ggtcgtctcg ttcgatgggc agcgcgaggt cgcggtcgag
      901 gtcggggagt gggtcgaggt tcgcgccctg cccgtgccgt tgcgcctggt cgagcccgag
      961 ggcagcaccc cgttctacga cctgcttcgg acgaaggcgt ccctgctccc tgactgatat
     1021 gtctggtcca cgcccatgac caccgccgcg gccgaccctc gcctgacggc cgccatcggc
     1081 gagttcctgc tcgagctgcg ggtggaacgc gggctctcgc ccttgaccat cgccgcctac
     1141 cgccgcgacc tcgatcagtt cgcggccggc tccgggccgg gctggcgcga cgacccggcg
     1201 gtggtgggcg agttcgtggc gcgcctccgg cggcagggcc gacgggcgtc gacccaggcc
     1261 cgcaaggtgg cggcgatccg gagcttctac ggcttcgccc ggcgggaggg tgtgatcgag
     1321 cgggatctcg ccgacctcat cgacccgccc cgccccggtc gctacctgcc cgaggtgctc
     1381 gcgccggacg aggtcgaacg gatcttggcc gccccgggtg acgacccggc cgggctacga
     1441 gatgcggcca tcctcgagct gctgtacgcg tgtggtctgc gggtgtccga gctgaccggg
     1501 ctcgacctcg accggctgaa cctgggtgcg ctcgaggttc gagtcatcgg caaggggaac
     1561 cgcgagcgcc gggtgccgat gggcgagcct gcccgcgacc ggctccatcg gtatctgtcc
     1621 ggcccgcgtc aggcctggac ggccggcaag ccgacgccgg cggtgttcgt gtcgcagcgc
     1681 ggaacgcggc tcgtccggga atcggtgtgg cggctggtgc gacgctggag ccaggcggcg
     1741 ggggtcgtgg gagacatcac cccgcacaca ttccggcaca gctttgcgac ccatctcttg
     1801 gagggaggcg ccgatctgcg ggtggtccag tcgctcttgg gccatgctag catcagcact
     1861 acccagctct atacccacct gaccggcgag cgcgttcggg aggtgtacac ccgggcgcac
     1921 ccgcgagcct gaggagaccg atgagttacg cgcgcaagct gctggcccga ggcgaggacg
     1981 tcgtcctgga gagccaccag cactggttcg tcgtcatcgg ccgatcgtgg tgggcgatcg
     2041 tgctcgccat cttcgccctg gcggtcctga tcttcgtggc cagcccaccc gaggagggta
     2101 ccgacagcgc ggccgaggtc gtggccttca tcttcctgct catcgcgttc gctcgcatcg
     2161 gatgggtgat ctgggactgg cggaacaacg agttcctggt caccacccgc cggatcgtgc
     2221 gcgccgaggg gatcctgaac aagcggatgg tcgagaccag cctcgagaag gtgaatgacg
     2281 ccgtgctcac ccagagcatg ttcgggcggc tgttcggttt cggcgacctc gacatcctga
     2341 ccgccgccga cgagatgggc ggcattgagg acttcccgat ggtcgccgac ccggtcgagt
     2401 tcaaggtcgc gatgatgaac cagaaggaag cggtcgagcg gccggacatc gccccgcagg
     2461 cctaccagca ccagaccgcc ccggaactgg tccccgccga gccgatggct cctcgcgccg
     2521 gaagcgaccg ggtaacggtg gtggacgagc cagcgcccgc ggcccaggct ccgatggaga
     2581 tgccggtcga cagccctccg agcagtcccg ctgcggccgc gccggccgag gacgccaccg
     2641 cgacactcga acggcttgcc tcgctgcgtg atcggggcct gatcacgccc gaggaatacg
     2701 acgccaagaa gcgcgagctc ctggagcgga tctagcgtgg ccgcggaccc ggccagcgaa
     2761 gacctcggac ggcggatcga gaccgccgcc gcagccatcc gcgagcgact gtctgcggtc
     2821 cccgctctcg cgacgcctcg gctcggactt gtcctcggct cgggcctcgg agccgtggcg
     2881 gacctcctgg atcctgagcc ccgcatccgg attccatacg gcgagatccc cgaagtgccg
     2941 gtgtccggcg tgcagggtca cgctggagag ctgatcgccg gactggtcgc cgggaagccc
     3001 gtgctcgtgc tggccggccg cgtgcacgcc tacgagggct acagccatcg cgaggtgacg
     3061 atcctgatca gagccacctt ccgggtgggt gtcgaggcgg tcgtgctgac caacgcggcc
     3121 ggtggcctgg acccggactt cgagcccggt gagctcatgc tctg
//
LOCUS       DASYGU010000212         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1319982, whole genome shotgun sequence.
ACCESSION   DASYGU010000212 DASYGU010000000
VERSION     DASYGU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319982"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VGO32_05955"
     CDS             <1..775
                     /locus_tag="VGO32_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200329.1"
                     /translation="DGIMLAYDPSKGDAIVLGLEPSDPILTDVRGIVMEVNRGAVTIA
                     VPAVMLAGVAGSGAAVHGPLTIAVTNAEAPLEPAMIDADAAGRIMVGGSWASAEVITR
                     ARAVGVAGIIVGGLHARELAEFTGLQHRRSLLGTAAPPFAVLGLDGFGRSTMDPARFA
                     WLQANADQPATILGDQRRLLVYDAAPAPARVARATIGDRVVIASGPGRGQAGLLTEVP
                     GQPRAIGSGISALCGLVRLDAGRTVAVALANLEASPSLS"
     gene            788..1288
                     /gene="tsaE"
                     /locus_tag="VGO32_05960"
     CDS             788..1288
                     /gene="tsaE"
                     /locus_tag="VGO32_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV7200330.1"
                     /translation="MTARNPRAAVCGQLITHSAAATRQIGRRLGRAAEPGTCLALVGP
                     LGAGKTQLAKGVALGLDVAGIVNSPTFIIVNEHPGRLILFHVDAYRLTDADEGRQAGV
                     FDERQADGVTVIEWADRLDGWLPPDRLSIELGTHPEEADWRVLRWQATGPRHAALGRV
                     LEAANA"
     gene            1285..1920
                     /gene="tsaB"
                     /locus_tag="VGO32_05965"
     CDS             1285..1920
                     /gene="tsaB"
                     /locus_tag="VGO32_05965"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV7200331.1"
                     /translation="MIIAIESASPDQSVALAHPSGELVADAAWSAERGQGSLLLPRLL
                     ELMSDHGVPMSAVSSLAVGLGPGSFTGLRVGLALAKGLAAGLAVPIVGVPSLDAWLDA
                     QPTAVGAMVRAGAAQAWIRARTEPEPRLIAFDAVAETSRDAELVASTDLIGALELRRA
                     SPPTRAAAAVARRAAERLRAGAADDLASLEPVYLRPPRGLDDRAAAPVTWL"
     gene            1911..2447
                     /gene="rimI"
                     /locus_tag="VGO32_05970"
     CDS             1911..2447
                     /gene="rimI"
                     /locus_tag="VGO32_05970"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV7200332.1"
                     /translation="MALTAAVRITPMVVDDIPAVLEIERASFLTPWPPEAFQQEVKHN
                     RLARYAVARQGSVVVGYAGVWLMVDEAHITTFAVHPDWRRQGVGRRLLLGMLSVAEEL
                     GATKLTLEVRVSNVAAQGLYAELGFAIAGVRERYYTDDGEDAYIMTTPPLTDDAMHGP
                     LEAARLALAEDAGEDVVI"
     gene            2444..3508
                     /gene="tsaD"
                     /locus_tag="VGO32_05975"
     CDS             2444..3508
                     /gene="tsaD"
                     /locus_tag="VGO32_05975"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV7200333.1"
                     /translation="MSRGPRILAIETSCDETGIAVVSDGRWIESNVVASQVAVHAVTG
                     GIVPEVAARQQLRWILPGLEMALRKADVGWDDLDAIAVTYGPGLIGSLLVGINVAKGL
                     AVSHGLPLVAVNHIEGHIYANWLAEPPSDDGANAKMAVEPAEPPFPLLCLVVSGGHTQ
                     LVLMADHGQYRLLGQTVDDAAGEAFDKVGRLLGLPYPGGPAISAAAEGVAPSTRFPLA
                     RAAGRYDFSFSGLKTAVLREVEGYRSRGEPIPVDGIAAAFERAVVDQLVRRTVAAAAE
                     HEVAAVALGGGVAANRALRQALAERLGGLPLLVPPPAWCTDNGAMIGAAAAHRFAAGE
                     RADAALEAIPSLALPWQPAA"
     gene            3508..4377
                     /gene="rsmA"
                     /locus_tag="VGO32_05980"
     CDS             3508..4377
                     /gene="rsmA"
                     /locus_tag="VGO32_05980"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV7200334.1"
                     /translation="MGTRTEDPLRPPTPGELRRLLREEGLRPRRGLSQNFLTDGDALD
                     TIVSAADLQAGDRVVEIGPGLGVLTRRLIAAGCEVVAIEVDAHLIGYLQRELDDAAGL
                     TLINADALAVDPADLFPRERYRLVANIPYHITSPLLHQFLASGHPPDLAVLLVQAEVA
                     ERIASPPGGMSYLSVFVQDLAEAEIVARVPASAFEPEPEVASAVLRLIRRPEPLVPPE
                     DREPFHRLVQAAFRQRRKQIHNSLVRELPVDRPVVESALSACAVTPERRPQTLSVPEW
                     ACLTTQLREALRE"
BASE COUNT          578 a         1654 c         1624 g          667 t
ORIGIN      
        1 ggacgggatc atgcttgcct acgacccgtc gaagggcgac gccatcgtgc tgggcctgga
       61 gccatccgat ccgatcctga ctgatgtccg agggatcgtg atggaggtca accgcggcgc
      121 cgtcacgatc gccgtgccgg ccgtcatgct ggccggggtg gcgggtagcg gggcggcggt
      181 ccacggtccg ctcacgatcg ctgtcaccaa cgccgaagcg cccctcgagc cggccatgat
      241 cgacgccgac gccgcgggcc ggatcatggt cggtgggtcg tgggcctcag ccgaggtcat
      301 cacccgggct cgggcggtcg gcgtggccgg catcatcgtc ggcggcctcc acgcccggga
      361 gctggccgag ttcaccggcc tccagcatcg ccgcagcctg ctcggcaccg ccgccccgcc
      421 ctttgccgtc ctgggccttg acggcttcgg ccggtcgacc atggaccccg cccggttcgc
      481 ctggctgcag gccaacgccg accagccggc caccatcctg ggcgaccagc ggcggctcct
      541 cgtgtacgac gcggccccgg ccccggcgcg ggtggcccgc gcgacaatcg gcgaccgcgt
      601 ggtgatcgcc tcggggcccg gacgaggcca ggccgggctc ctgaccgagg tccccggaca
      661 gccgcgggcc atcggttcag ggatcagcgc gctctgcggt ctcgtccgtc tggacgccgg
      721 ccggaccgtc gccgtcgcgc tcgccaacct cgaggccagc ccgtcgctgt cctgacatac
      781 tcgccgcgtg accgcgcgga acccccgggc cgcggtctgc ggccagctca tcacccattc
      841 ggccgccgcg acgcggcaga tcggccgtcg gctgggccgg gccgcggagc cgggcacctg
      901 cctggccctg gtcggccccc ttggcgcggg caagacccag ctggccaagg gggtcgcgct
      961 cggccttgac gtggcgggga ttgtgaacag cccgacgttc atcatcgtga acgagcatcc
     1021 gggccgactg atcctgttcc acgtcgacgc ctatcgcctg accgatgctg atgagggtcg
     1081 ccaggccggg gtcttcgacg agcggcaggc cgacggcgtg accgtcatcg aatgggccga
     1141 tcggctcgat ggctggctgc cgccggatcg gctctcgatc gagctcggga cgcatccaga
     1201 agaggccgat tggcgcgtcc tgcgctggca ggccaccggt ccccgccacg ccgcgctggg
     1261 ccgcgtcctc gaggccgcga acgcgtgatc atcgcgatcg agagcgcgtc tccggaccag
     1321 tcggtggcgc tcgcgcaccc gtccggcgag ctcgtggcgg atgccgcctg gagcgccgag
     1381 cgcggccagg gcagcctgct cctccctcgt ctgctggagc tcatgagcga ccacggggtg
     1441 ccgatgagcg cggtcagcag cctggcggta gggctcggcc cgggatcgtt caccgggctc
     1501 cgggtcgggc tggcgctggc caagggcctg gccgccggac tggctgtgcc gatcgtcggc
     1561 gtgccgagcc ttgatgcctg gcttgacgcg cagccaaccg cggtaggggc catggtccgt
     1621 gccggcgccg cgcaggcgtg gattcgcgcc cggacggagc cggagccccg gctgatcgcc
     1681 ttcgatgccg ttgccgagac ctcccgtgac gcggagctcg tcgcctccac cgatctcatt
     1741 ggcgcgctcg agctccgacg ggcgtctccg ccgacccgtg cggcggccgc cgtcgctcga
     1801 cgggccgccg aacgtctgcg cgccggcgct gcggacgacc tggccagcct cgaaccggta
     1861 tacctccgtc cgccccgcgg tctcgacgac agggcggcgg cccccgtcac gtggctctga
     1921 ccgccgccgt tcggatcacg ccgatggttg tggacgacat cccggccgtg ctcgagatcg
     1981 agcgggccag cttcctcacc ccgtggccgc cggaggcctt ccagcaggag gtgaagcaca
     2041 accggctggc ccgctacgcg gtggcgcggc agggatcggt ggtggtcggg tacgccggcg
     2101 tgtggctgat ggtcgacgag gcccacatca cgacgtttgc ggtgcatccc gactggcgac
     2161 ggcagggtgt cgggcgacgt ctcctgctcg gcatgctgtc ggtggccgag gagctgggtg
     2221 cgacgaagct gacactggag gtccgggtct cgaacgtcgc cgcccagggg ctctacgcgg
     2281 agctcggatt cgcaatcgcc ggcgtccgcg agcgctacta caccgatgac ggcgaggacg
     2341 cctacatcat gaccaccccg ccgctcaccg acgacgcgat gcacggcccg ctggaggccg
     2401 cccggctggc gttggccgag gacgccggcg aggacgtcgt catatgagcc gtgggcctcg
     2461 gatcctcgcg atcgagacca gctgcgacga gacagggatc gcggtcgttt ccgacgggcg
     2521 gtggatcgag tcgaacgtgg tcgccagcca ggtcgcggtc catgccgtga cgggcgggat
     2581 cgtgccggag gtcgcggccc gccagcagct gcgctggatc ttgcccggtc tcgagatggc
     2641 cctccgcaag gccgacgtcg gctgggacga cctggatgcc atcgcggtca cctacggacc
     2701 gggcctcatc ggctcgttgc tggtcgggat caacgtggcc aagggtctcg ctgtcagcca
     2761 cgggctgccg ctggtggcgg tcaaccacat cgaagggcac atctacgcga actggcttgc
     2821 cgagccgcca tccgacgatg gcgcgaacgc gaaaatggcg gtcgagcccg cagaaccgcc
     2881 gttcccgctc ctgtgcctgg tcgtttcggg cggccacacc cagctggtgc tcatggccga
     2941 tcacgggcag taccgcctcc tgggtcagac cgtggacgac gcggcaggcg aggccttcga
     3001 caaggtcggc cgcctgctcg gcctcccgta cccgggcggg ccggcgattt cggcggctgc
     3061 cgaaggggtc gccccttcga ctcgcttccc gctggccagg gccgcgggcc ggtacgactt
     3121 ttcgttctcc gggctgaaga ccgcggtgct gcgcgaggtc gagggttatc ggtcgcgcgg
     3181 tgagccgatc ccggtggacg gcatcgcggc ggccttcgag cgcgcggtgg tggaccagct
     3241 cgtccgccgg accgtggccg cggcggccga gcacgaggtc gcggccgtgg cgctgggcgg
     3301 cggcgtggcg gccaaccgag ccctgcgcca ggcgctcgcc gagcgcctgg gcgggctgcc
     3361 gctgctcgtg ccgccgcccg cgtggtgcac cgacaacggc gcgatgatcg gcgccgcggc
     3421 ggcccaccgg tttgcggccg gggaacgcgc ggacgccgcg ctcgaagcca tcccgtcatt
     3481 ggcgctgccc tggcagcccg ccgcctgatg gggacaagga ccgaggaccc actccgcccc
     3541 ccgacgcccg gcgagctccg ccgtctcctc cgcgaggagg ggctacgccc ccgtcgcggg
     3601 ctgagccaga acttcctcac cgacggagac gccctcgaca cgatcgtctc cgccgccgac
     3661 ctgcaagccg gggatcgggt cgtcgagatt ggcccgggcc tgggggtgct cacccgtcga
     3721 ctgatcgcgg ccggctgcga ggtggtcgcg atcgaggtgg acgcgcacct gatcggctac
     3781 ctccagcgcg agctggacga tgccgcgggc ctgaccctga tcaacgccga tgcgctggcc
     3841 gtggacccgg ccgacctgtt tccccgcgaa cggtaccgat tggtggcgaa catcccctat
     3901 cacatcacca gcccgctcct ccaccagttc ctcgcgtcgg gtcacccgcc cgatctcgcc
     3961 gtcctgctcg tccaggccga ggtcgccgaa cggatcgcct cgccgcccgg cgggatgagc
     4021 tacctgtcgg tgttcgttca ggacctcgcc gaggccgaga tcgtggccag ggtgcccgca
     4081 tccgccttcg aacccgagcc ggaggtcgcc tcggccgtgc tgcggctcat ccgtcgaccg
     4141 gagccgctcg tcccgcccga ggaccgcgag cccttccatc gtctcgtcca ggccgccttc
     4201 cgacagcgcc gcaagcagat ccacaacagc ctggttcgtg agctgccggt cgatcggccc
     4261 gtggtggaga gcgcgctctc ggcctgcgcc gtgacccccg agcggcggcc gcagaccctg
     4321 tcggtgccgg aatgggcgtg cctcacgacg cagctgcgcg aggcccttcg tgagtgagga
     4381 ccgatcgcgg cgacggctcg ccgccccggc caaactcaac ctctcgctgc gcgtcgtcgg
     4441 ccgccggtcg gacggcttcc acgagctcga atcgctcatg gtcttgctcg agctggcgga
     4501 catcgtctcg gcgggcgggg acg
//
LOCUS       DASYGU010000213         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1577241, whole genome shotgun sequence.
ACCESSION   DASYGU010000213 DASYGU010000000
VERSION     DASYGU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577241"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGO32_05985"
     CDS             <1..301
                     /locus_tag="VGO32_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEV7200335.1"
                     /translation="TETIRRVTVVSAAGLSGTLEVRDGPTAVELDEDGVPRAGVSGQD
                     QAAGTRITLSADERIRLRVGDAGRVHLVLNGYVISPMGDAGATVEWAIRALVPGG"
     gene            311..799
                     /locus_tag="VGO32_05990"
     CDS             311..799
                     /locus_tag="VGO32_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV7200336.1"
                     /translation="MADSSFDVVSDFDQQELVNALDQVRREIATRYDFKNSKCTLELG
                     KTDITLVADDDFRAAAVKDLVQSKAVKRGLSLKIFDWGKAEPAAGGTVRQVIALKRGL
                     TADQAKELSKHVRDHFPKVKVSIQGDALRVISPKKDELQGVIADLRALDYPVALQFIN
                     YR"
     gene            796..1299
                     /locus_tag="VGO32_05995"
     CDS             796..1299
                     /locus_tag="VGO32_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEV7200337.1"
                     /translation="MSGPAEIDLESAAARLGEDLLAGEWTLVTAESSTAGLIGHAITM
                     VPGASRYYLGGVIAYSNLAKELELGVDHETLVAHGAVSEPVAAAMAEGVRARFDADLG
                     VAVTGIAGPDGGGADKPVGLHFVGASRRGHPATVERHVFQHDRDGNKAAAALAALELA
                     RREIRAG"
     gene            1299..2711
                     /gene="murC"
                     /locus_tag="VGO32_06000"
     CDS             1299..2711
                     /gene="murC"
                     /locus_tag="VGO32_06000"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV7200338.1"
                     /translation="MVAPGERIHVIGIAGAGAAGTALLAAHAGARVDGCDAAGPSSYT
                     EPLTPLGVTVATGHDPSHLERVERVAVSAALRLQPDLPELAAARERGIPVVPWQALLG
                     EMMGERGRLGVAVAGTHGKSTATALLGHLLLAAGHDPTVEVGAMIPAWGASARSGGGV
                     AFLTEADEYDGNFLHLHPAAVLLTTIEMDHPDVFANEDAVYDMFVRFLSAMSSHELVG
                     GKLLVALAGDSGTEAVLARLESWDGRLVEYGPGTDIAAANVTFDQDGTSFDLFGRRFR
                     SRLAGAHNVLNAIGALVMAHELGIGPEALQEALEGFGGASRRLELITDTAGVLVFDDY
                     AHHPTEVRAALDGARQRVGDRRLWAVIEPHTFARTRAMFDEYAASFKSADEVVVADVF
                     AARDPDTSIVSAEELADAIERVSAVPAYASGSVAETGDYIGYKLRPGEAVVTLGIGSS
                     HKIAARIAEILEEQVAEGIF"
     gene            2717..3088
                     /locus_tag="VGO32_06005"
     CDS             2717..3088
                     /locus_tag="VGO32_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200339.1"
                     /translation="MRAVVLTILLAAGVAVVQQGLVRLVEGSGAAAEPAVILILLLAA
                     FLGLLTGVATRALNLPGAVAPTALVMAWTVAPVIFAALPWEIFQALGGDAAIDPTESL
                     ALAVAAIAAAVTLGVQIPNSD"
     gene            complement(3215..>4208)
                     /gene="hflX"
                     /locus_tag="VGO32_06010"
     CDS             complement(3215..>4208)
                     /gene="hflX"
                     /locus_tag="VGO32_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV7200340.1"
                     /translation="IFARHARTREGRLQVELAQLEYHLPRLTRMWTHLSRTGGGIGTR
                     GPGESQLETDRRRIREKIHRLRDEIAQVKRHRATAGRRRDRSEVPVVALVGYTNAGKS
                     TLLNALTDADAFVADMPFATLDPTSRRITFESHRHVVLTDTVGFINKLPHDLVDAFRA
                     TLEEVLRADLLLEVVDASDPDFIAQQETVQTVLDELGAGDTPRIRVFNKLDRLNGALA
                     TAGPPPSDDDVAYVSALSGVGLPALRERIALALRGRLVPVDAVVPYPRAELLARARSA
                     GEVTERFTDDGVRIVGHLPESLAAEVRAAANGSRSAPAAPRSAPRTPRAASQRS"
BASE COUNT          639 a         1420 c         1503 g          646 t
ORIGIN      
        1 gaccgagacc attcgccgcg tgaccgtggt ctccgccgcg gggctgtccg gcacgttgga
       61 ggttcgcgac gggcccaccg ccgtggagct ggacgaggac ggcgtgcctc gggccggcgt
      121 atcgggccag gaccaggcgg cggggacgcg gatcacgctc agcgccgacg agcgcatccg
      181 gcttcgagtg ggggatgcgg gtcgcgtgca cctcgtcctg aacggatacg tgatctcgcc
      241 catgggtgat gccggggcga ccgtcgagtg ggccatccgc gccctcgtgc cgggcggcta
      301 gcattaggac atggccgact cctccttcga cgtggtcagc gacttcgacc agcaggagct
      361 ggtgaacgcg ctggaccagg tccgtcgcga gatcgcgacc cgatacgact tcaagaattc
      421 caagtgcacc ctcgagctgg gcaagaccga tatcaccctc gtcgccgatg acgacttccg
      481 cgccgccgcg gtcaaggacc tcgtccagtc caaggccgtc aagcgcggcc tcagcctgaa
      541 gatcttcgac tggggcaagg ccgagccggc ggccggcggc accgtccgcc aggtcatcgc
      601 gctcaagcgc ggcctgactg ctgatcaggc caaggagctg tccaagcacg ttcgggacca
      661 cttccccaag gtcaaggtct cgatccaggg cgacgcgctg cgggtcatct cgcccaagaa
      721 ggacgagctc cagggggtca tcgccgacct ccgcgcactg gactacccgg tcgcgctcca
      781 gttcatcaac taccgatgag cgggcctgcc gagatcgatc tcgagtcggc cgcggcccgg
      841 ctgggggagg atctgctggc cggcgaatgg actctggtca cggccgagtc gtccacggcg
      901 gggctcatcg gtcacgcgat cacgatggtg cccggcgcca gccgctatta cctcggcggc
      961 gtgatcgctt actcgaacct ggccaaggag ctcgagctcg gggttgacca cgagacgctc
     1021 gtggcccacg gcgcggtcag cgaaccagtc gccgcggcca tggccgaggg ggtcagggcg
     1081 cggttcgatg ccgacctggg cgtggccgtg accggcatcg ccgggcccga cggcggggga
     1141 gcggacaagc cggtcggcct ccatttcgtg ggtgcctcgc gacggggaca tccggccacc
     1201 gtcgagcgcc acgtcttcca gcacgatcga gacggcaaca aggccgccgc cgcactcgcc
     1261 gccctcgagc tggcgcgccg ggagatccgc gccggctgat ggtcgcgccc ggcgagcgga
     1321 tccacgtcat cggcattgcc ggcgccggag ccgccgggac cgccctgctg gcggcgcacg
     1381 ccggggcccg ggtggacggc tgcgatgccg ccggcccgtc ctcgtacacc gagccgctaa
     1441 cccctttggg cgtcacggtg gctactggcc acgatccatc tcatttggaa agggttgagc
     1501 gggtcgcggt cagcgccgcg ctccgcctcc aaccagacct gcccgagctc gccgccgcgc
     1561 gagaacgggg gattccggtc gtcccgtggc aggccctcct gggcgagatg atgggggagc
     1621 ggggccggct gggggtggcc gtcgccggca cgcacggcaa gtcgaccgcc accgcgctcc
     1681 tcggccacct gctcctcgcc gccggacacg atccaacggt ggaagtcggg gcgatgatcc
     1741 cggcctgggg cgcctcggcc aggtccggtg ggggagtggc gttcctcacc gaggccgacg
     1801 agtacgacgg caacttcctc cacctccatc ccgcagccgt cctgctgacc acgatagaga
     1861 tggaccatcc cgacgtcttc gccaacgagg acgcggtgta cgacatgttc gttcgcttcc
     1921 tgagcgcgat gagcagccac gagctggtcg gtggcaagct tctcgttgcc ctggcggggg
     1981 attcggggac cgaagcggtc ctggctcggc tggagagctg ggacgggcgc ctggtcgaat
     2041 acgggccggg taccgatatc gcggcagcca atgtcacctt tgaccaggac ggcaccagct
     2101 tcgacctgtt tgggcggcga ttccgatcgc gccttgccgg cgcgcacaac gtgctcaatg
     2161 cgattggcgc cctggtgatg gcccacgagc ttggaatcgg tcctgaggcg ctgcaggagg
     2221 cactggaggg cttcggtggg gcgtcccggc ggctggagct gatcaccgat actgccggag
     2281 tgctcgtgtt cgacgactac gcgcaccatc cgaccgaggt gcgggccgcg ctggacggcg
     2341 cccggcagcg ggtgggcgac cgacggctgt gggcggtgat cgagccgcac acgttcgcgc
     2401 ggacccgcgc gatgttcgat gagtacgccg cgtccttcaa gtcagcggat gaggtcgtcg
     2461 tagccgatgt cttcgccgcc cgcgaccctg atacgtcgat cgtgagcgcg gaggagctgg
     2521 cggacgccat cgagcgggta tcggcggtgc ccgcgtacgc gtcgggaagc gtggccgaga
     2581 ccggcgacta catcggctac aagctccgtc caggagaggc cgtggtcacg ctcgggattg
     2641 gctccagcca caagatcgcg gcgcgaatcg ctgaaatcct cgaggaacag gtggcggagg
     2701 gcatcttttg agaggcgtgc gcgcggttgt cctcacaatc ctgctggcgg ccggcgtcgc
     2761 cgtcgttcag cagggcctgg tccggctggt cgagggttca ggagcggccg ccgagcccgc
     2821 ggtcatcctg atcctcctgc tcgcggcctt tctgggcctc ctcacaggcg tcgccacccg
     2881 tgcgctgaac ttgcccgggg ccgtggctcc gacggccctc gtgatggcgt ggaccgtcgc
     2941 tccggtcatc ttcgcggctc tgccgtggga aatcttccaa gccctcggcg gtgacgcggc
     3001 catcgatccc accgaatccc tagccctcgc ggtcgccgcg atcgctgccg ccgtcactct
     3061 cggtgtccag atccccaaca gcgactaggc gcgctccgaa gtcaattccg cgcaatgtac
     3121 gctcgtgggt gcgaggctaa cgcaacataa cctccatttg cggaagcgct aatccggcac
     3181 cgggggaggc atcggagatg ccaggagtgg tggtctagga gcgctgcgag gcggcgcgag
     3241 gagtccgcgg ggctgatctg ggcgccgcgg gcgccgatct ggacccgttg gcggccgcac
     3301 ggacctcagc ggccagtgat tcaggcagat ggccgacgat gcgcaccccg tcatcggtga
     3361 accgctccgt gacctcgccc gcggagcggg cccgggcgag gagctcggcc cgcggatatg
     3421 ggacgacggc atccaccggc accaggcggc cacgaagcgc gagggcgatc cgctcgcgca
     3481 gggccggcag gcccacgcca ctcagggcgc tcacgtaggc gacgtcatcg tccgacggcg
     3541 gcgggccggc cgtcgccaac gccccgttca ggcgatcgag cttgttgaag acccggatcc
     3601 gcggcgtgtc gccggcccca agctcgtcga gcacggtctg cacggtctcc tgctgggcga
     3661 tgaagtccgg atccgaggca tccaccacct cgagcaggag atcggcgcgc aggacctctt
     3721 ccagcgtggc tcggaaggca tccaccaggt catgaggcag cttgttgatg aaccccacgg
     3781 tatcggtcag gacgacgtgg cggtgcgact cgaaagtgat ccgccgagaa gttggatcca
     3841 gggtggcgaa cggcatgtcg gccacgaatg cgtcggcatc ggtcagggcg ttgaggagcg
     3901 tggacttgcc cgcgttcgtg tagcccacca gggccacgac cggcacctct gaccgatccc
     3961 ggcgacggcc cgcggtggcg cggtggcgct tgacctgcgc gatctcgtcg cggagccgat
     4021 ggatcttctc gcggatgcgc cgacggtcgg tctcaagctg gctctcgccc ggtccccggg
     4081 tgccgatccc gccgccggtc cgcgacaggt gagtccacat ccgggtcagg cgtggaaggt
     4141 ggtactcgag ctgggcgagc tcgacctgga gccggccctc gcgagtgcgg gcatgacggg
     4201 cgaagatg
//
LOCUS       DASYGU010000214         5238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_137113, whole genome shotgun sequence.
ACCESSION   DASYGU010000214 DASYGU010000000
VERSION     DASYGU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137113"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="VGO32_06015"
     CDS             <1..2004
                     /locus_tag="VGO32_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV7200341.1"
                     /translation="SSKTPTLDQLGINLTELARSGKLDPVIGREKEIERVIQILGRKT
                     KNNPALIGEPGVGKTAIAEALAHRIVSGDVPDTLLDKRVLTLDIGSLVAGTKYRGEFE
                     ERLKKIIEELRSSSDSVLFIDELHTLVGAGAAEGAIDAANILKPPLSRGELQCIGATT
                     LDEYRKYIEKDAALERRFQPVMVDEPTVDQANEILLGIRTRYEEHHHVKYTDEALRAA
                     VDLSVRYVADRALPDKAIDLIDEAGSRVRLRHASAPPSVKEAQQDLEEIIKRKDEAIA
                     AQEYEGAARLRDEEAAGRERIDELRKAWHEEAAKETPEVDDEEIAQVVSMWTGIPVVR
                     ISQEEKDRLLQMEDALHKRVIGQDEAIRTISSAVRRARAGLKDPKRPIGSFVFLGPTG
                     VGKTELARALAEFIFGSEEALVKLDMSEFMERHNVSRLVGAPPGYVGYDEGGQLTEAV
                     RRKSYSVVLLDEIEKAHPEVFNILLQILEDGQLTDAKGRRVDFRNTIVIMTSNVGAAL
                     LQRDSSIGFRAGGDAAAEEIAEYDRMKEKVLGELKNLFRPEFLNRIDASVVFRSLTRE
                     EIRSIVDLQLARVLEQVAAQQMELTVTDAAKDAIIEKGFDQAFGARPMRREIQNQIED
                     PLAERLLQAEFGPGDKILVDAGPDGGLIITKKSPRKQPVKVAN"
     gene            2167..3213
                     /locus_tag="VGO32_06020"
     CDS             2167..3213
                     /locus_tag="VGO32_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV7200342.1"
                     /translation="MQNIVRLSGALLGIVLGFGLGLLILDRTDDLIAPANRPAFLVAI
                     VVAALLFGYLAIPYVTLYPTRWAVGRLSEAEAADFVLGVIGLLIGLLMGALIGIPLSG
                     LGGGLGSWVPVVVSLALAAIMAGAFMYKREAILKAIGSVGSAGRRGVPNKVVVDTSAV
                     IDGRIVDIAKTGFILGALVAPRFVLDELQRIADSPDAIRRNRGRRGLEMLSALQKDSV
                     TPVEISEATYSELDEVDAKLVALARDTGAAILTNDYNLNRVAELQGIRVLNINELANA
                     VKAVVHPGEELSVQIIQEGKEPGQGVGYLEDGTMIVVEGGSRFINHEVPISVTRVLQT
                     VAGRMIFAQPRTDE"
     gene            3210..4442
                     /gene="ispD"
                     /locus_tag="VGO32_06025"
     CDS             3210..4442
                     /gene="ispD"
                     /locus_tag="VGO32_06025"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV7200343.1"
                     /translation="MTGGTAPRAGVLVGAGRGRRMGEEADKLWLEALGRTIWRWSLDA
                     LLAVPGMTTVAVVVPADAMDRWAAAIPAAATDRCRLVAGGEARVDSVRAGLAALTDAG
                     VSDGATVLVHDAARPAASTALMAAVATAATGGAGAIPVAPVHDTLKRVREGMVVETVD
                     RTDVGAAQTPQAASLGILRAALDAARAEGLEPTDEAAALAAINVPVRAVAGDPANRKL
                     TDPDDLPLLQSTLRARAAPSVAPSGAAFAGGTGAVRAGVGFDAHRLADGRPLRLGGLA
                     FPDEPRGLLGHSDGDVALHAVIDALLGAAGLGDVGTLFPPDDPAWAGVDSGEMLRRAA
                     DHVRGSGWSPRSVDLAVATQRPAIAPRRDEMTARIAELAGLPPTAVTARGTTSDELGF
                     AGSEGIAAWSVAVVERTA"
     gene            4439..5188
                     /gene="rlmB"
                     /locus_tag="VGO32_06030"
     CDS             4439..5188
                     /gene="rlmB"
                     /locus_tag="VGO32_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV7200344.1"
                     /translation="MSEWIGGRRPVAEALSAGRPARRMLIARGVGPSPEMKALRSAAA
                     QASVPIEEVARDQLARLAGFDGHQGVLLEVEDRRWSDPMEILAVASASHRPPFILVLE
                     HLQDPVNLGAILRTAEAAGVDGVIFPERGSAPLSAAAVKSSAGASEHLRLAPVGSLGA
                     ALAELQSRDILLVAADQDAPVLAWDADLRGPMAVVVGGEGAGLSGPTRRRCDVLVRFP
                     MAGHVASLNAAAAGALLLFEVVRQRGLESPT"
BASE COUNT          811 a         1754 c         1898 g          775 t
ORIGIN      
        1 tcgtccaaga cgccgaccct cgaccagctg ggcatcaacc tgactgagct cgcgcggtca
       61 ggcaagctcg acccggtcat cggccgggag aaggagatcg agcgagtcat ccagatcctc
      121 ggtcggaaga cgaagaacaa ccccgccctc atcggcgagc cgggcgtggg caagaccgcg
      181 atcgcggagg cgcttgcgca tcggatcgtg tccggcgacg tgcccgacac cctgctcgac
      241 aagcgggtcc tgaccctgga cattggctcg ctggtggccg gcacgaagta ccgcggcgag
      301 ttcgaggagc ggctgaagaa gatcatcgag gagctgcggt cgagcagcga ctcggtcctg
      361 ttcatcgacg agctccatac cctggtcgga gcgggcgccg cggagggggc gatcgacgcc
      421 gccaacatcc tcaagccgcc gctgtcccgc ggcgagctcc agtgcatcgg cgcgaccacg
      481 ctggacgagt accgcaagta catcgagaag gatgcggcac tcgagcgacg cttccagccg
      541 gtgatggtcg acgagcccac cgtggaccag gccaacgaga tcctgctcgg gatcaggacc
      601 cgttacgaag agcaccacca cgtcaagtac acagacgagg cgctccgcgc cgcggtcgat
      661 ctgtcggtcc gctacgtcgc cgaccgggcg ctcccggata aggccatcga cctgatcgac
      721 gaggcgggtt cccgggtgcg gctccgccac gcgtcggcgc caccgagcgt gaaagaggcc
      781 cagcaggacc tcgaggagat catcaagcgc aaggacgagg ctatcgcggc gcaggagtac
      841 gagggtgccg cccgcctgcg cgacgaggag gctgccggtc gggagcggat cgacgagctg
      901 cgaaaggcgt ggcacgagga ggcggccaaa gagactcccg aggtcgacga cgaggagatc
      961 gcccaggtcg tcagcatgtg gaccgggatt ccggtcgtcc gcatcagcca ggaggagaaa
     1021 gaccgcctgc tgcagatgga ggatgccctc cacaagcggg tcatcggcca ggacgaggcg
     1081 atcaggacga tcagctccgc cgttcgccgc gcacgcgccg gcctgaagga ccccaagcgg
     1141 cccattggct cattcgtgtt cctcggcccg accggcgtgg gcaagaccga gctggcccgg
     1201 gcgctggccg agttcatatt cggctccgag gaagccctcg tgaagctgga catgagcgag
     1261 ttcatggaac gccacaacgt gtcgcgcctc gtgggcgcac cgccgggata cgtcggctac
     1321 gacgaaggcg ggcagctgac cgaggccgtg cgccgcaaga gctacagcgt ggtcctgctc
     1381 gacgagatcg agaaggcgca cccggaggtc ttcaacatcc tgctccagat cctggaggac
     1441 gggcagctga cggacgccaa gggccgtcgg gtcgacttcc ggaacacgat cgtgatcatg
     1501 accagcaacg ttggagccgc cctgctccag cgcgactcgt cgatcggctt ccgggccggt
     1561 ggcgacgcgg ccgcggagga aatcgccgag tacgaccgga tgaaggaaaa ggtgctgggc
     1621 gagctgaaga acctgttccg ccccgagttc ctgaaccgga tcgacgcctc ggtcgtgttc
     1681 cgcagcctga cccgggagga gatccgctcc atcgtcgacc ttcagctggc gcgcgtcctc
     1741 gagcaggtcg cggcccagca gatggagctg acggtgaccg atgcggccaa ggacgcgatc
     1801 atcgagaagg gcttcgacca ggcgttcggc gcccgaccga tgcggcgcga gatccagaac
     1861 cagatcgagg atccgctggc cgagcgtctg ctccaggccg agttcgggcc gggggacaag
     1921 atcctggtgg acgccggtcc ggatggcggc ctcatcatca ccaagaagag cccccgcaag
     1981 cagccggtga aggtcgcgaa ctgaccctcg gaccgggaca atccggtctg gactggtgcg
     2041 gaatggggtg ctagtgtggc cccctgtttc gacgggatcc cgcgcgatct tttcgtcctc
     2101 gtttcatcca tctatcgcat ctcgtgaccg gctcgcgcga cggccgctca atggacttat
     2161 cggtacatgc agaacatcgt ccggctgtcg ggcgcgctgc tcggcatcgt cttggggttt
     2221 gggttggggc tgctcatcct tgaccgcacc gatgatctga tcgcgccggc caaccggcca
     2281 gccttcctcg tcgcgatcgt ggtggccgcg ctcctcttcg gctacctggc gatcccgtac
     2341 gtgaccctgt accccacgcg atgggccgtc ggcaggcttt ccgaggccga ggccgccgac
     2401 ttcgtgctcg gcgtgatcgg gctgctcatc ggcttgctca tgggcgccct gatcgggatc
     2461 ccgctgtcgg gactcggggg cgggctgggc agctgggtgc cggtcgtcgt gtcgctcgcc
     2521 ctggccgcga tcatggccgg ggcgttcatg tacaagcgcg aggccatcct gaaggcgatt
     2581 ggcagcgtcg ggtcggcggg ccgccgtggc gtcccgaaca aggtggtggt ggatacgagc
     2641 gcggtcatcg atgggcggat cgtggacatc gccaagacgg gcttcatcct gggcgcgctg
     2701 gtcgcgcccc ggttcgtcct ggacgagctg caacggatcg cggactcgcc cgatgcgatc
     2761 cgccgaaacc gtggccggcg ggggctcgag atgctatccg cgctccagaa ggattcggtc
     2821 acgccggtcg agatcagcga ggccacgtat tccgagctgg acgaggtcga cgccaagctg
     2881 gtagcgctcg cacgcgacac gggcgctgcc atcctgacca acgactacaa cctgaaccgc
     2941 gtggcggagc tccagggcat ccgggtcctg aacatcaacg agctcgcgaa tgcggtgaag
     3001 gccgtggtcc atcccggtga ggagctcagc gtccagatca tccaggaagg caaggaacca
     3061 gggcagggcg tgggctacct cgaggacggg acgatgatcg tggtcgaggg tggcagccgg
     3121 ttcatcaacc acgaggtacc gatctcggtg acccgggtcc tgcagaccgt cgccggccgg
     3181 atgatcttcg cccagccgcg cacggacgag tgacgggggg cacggccccg cgggccggcg
     3241 tcctggtcgg ggccggccgc ggccgccgga tgggggagga ggcggacaag ctgtggctcg
     3301 aggcgctggg ccgaaccatc tggcgctgga gcctcgacgc gctcctcgcg gtcccgggaa
     3361 tgaccaccgt ggcggtcgtc gtcccagcgg atgccatgga ccgatgggcg gccgcaattc
     3421 cggctgccgc cactgaccga tgccgcctgg tcgccggtgg cgaggcacgg gttgattcgg
     3481 tgcgggccgg gctggcggcg ctgaccgatg ccggtgtgtc ggatggggcg acggttctcg
     3541 tccatgacgc ggcccggccc gcggcgtcga ctgccctcat ggccgccgtg gccacggcgg
     3601 caaccggcgg cgcgggtgcg atccccgtgg ctcccgtcca cgacacgttg aagcgggtgc
     3661 gcgaagggat ggtggtcgaa acggtggacc gaaccgatgt cggcgcggcc cagacaccgc
     3721 aggccgcgag cctgggcatc ctgcgtgccg cgctggacgc cgcccgcgcc gagggtctcg
     3781 agcccaccga cgaagcggcg gcgctggcag cgatcaacgt cccggtccgg gcggtggccg
     3841 gcgatccggc caaccgcaag ctcaccgatc ccgacgacct ccccttgctc cagtcaacgc
     3901 tgcgcgcgcg cgcagcgccc tccgtcgcgc cgtccggtgc ggcctttgcc ggggggactg
     3961 gcgccgtccg ggcgggcgtg ggcttcgacg cccatcgact ggcggacggc aggccgctcc
     4021 gcctcggcgg gctcgccttc ccggacgagc cgcgcggcct gctcggtcac tccgacggcg
     4081 atgtggcact ccatgcggtg atcgacgccc tgctcggcgc ggcgggactc ggcgacgtgg
     4141 gcaccctgtt cccacccgat gaccctgcct gggccggcgt cgactcgggc gagatgctgc
     4201 gccgtgccgc ggaccacgtt cgcgggtcgg gatggtcgcc acggagcgtg gacctggcgg
     4261 tggccaccca gcggcccgcg atcgcgcccc gtcgtgacga gatgaccgcc cgcatcgccg
     4321 agctggcggg gctgccgccg accgccgtca cggctcgagg aacgacgagc gacgagctcg
     4381 ggttcgccgg atcggagggc atcgccgcct ggtcggtggc cgtggtcgag cgcaccgcgt
     4441 gagcgaatgg atcggtggcc gccgtccggt ggccgaggcc ctgtcggcgg gtcgcccggc
     4501 gcgccggatg ctcatcgccc gcggtgtcgg tcccagtccc gagatgaagg cactgcggtc
     4561 ggccgcggcg caggcatcgg tcccgatcga agaggtcgcc cgcgatcagc tggcccgtct
     4621 cgccggattc gacgggcacc agggcgtgct gctcgaggtc gaggatcggc gctggtccga
     4681 tccgatggag atcctggcag tcgcgtcggc ctcgcatcgg cccccgttca tcctagtcct
     4741 ggagcatctc caggatccgg tcaacctggg cgccattctg cgcaccgcgg aagcggccgg
     4801 ggtggacggg gtgatctttc ccgagcgcgg gtcggcgccg ctcagtgcgg cggcggtgaa
     4861 gtcctcggcc ggcgcaagcg agcacctccg gctggcgccg gtcggcagcc tgggcgcggc
     4921 cctcgccgag ctccagtcac gggacatcct cctggtggcc gccgaccagg acgccccggt
     4981 cctcgcctgg gacgcggatc tccgtggacc aatggcggtc gtggtcggcg gtgagggagc
     5041 gggcctcagc ggtccgaccc gtcggcgttg cgacgtgctc gtccgcttcc cgatggccgg
     5101 ccacgtggcc agcctcaatg ccgccgcggc cggggcgctc ctcctgttcg aggtcgtgcg
     5161 gcagcggggt ctagaatcgc cgacctgagg ccgacgtagc tcaatggcag agcagcggtt
     5221 ttgtaaaccg ccggttgt
//
LOCUS       DASYGU010000215         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_137206, whole genome shotgun sequence.
ACCESSION   DASYGU010000215 DASYGU010000000
VERSION     DASYGU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137206"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VGO32_06035"
     CDS             complement(<1..638)
                     /locus_tag="VGO32_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200345.1"
                     /translation="MSSPRLVALRELAEEAELERRDFVSEATEQLRRFLDANRERLTE
                     IGPITLVDDEQDFLRFHPIDQLWVTRLTYQDPADGEWYDQEQEVDTLSELIELYNPAD
                     ILAWLIEASRNPIDHLTPANELAAEEAPPPETEDDNWQNELPADQRETLAAQRLWQLA
                     DAYRSQLQEQQAALLREFVGNAEDALRLTGDLVLLDESDDQLVMRGDGWFETT"
     gene            complement(635..967)
                     /locus_tag="VGO32_06040"
     CDS             complement(635..967)
                     /locus_tag="VGO32_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200346.1"
                     /translation="MPLMVTFFNVRHGVLRDTMFKRGMRHMFPSRVRDYEEKYGIKFI
                     GWFNVTEGSEWNNVIIFDLPNYEVLDQLYQDPATRRLGHNVAESIFDTKHTIFLRERM
                     GPDLVYKP"
     gene            complement(972..1307)
                     /locus_tag="VGO32_06045"
     CDS             complement(972..1307)
                     /locus_tag="VGO32_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200347.1"
                     /translation="MAIRTNEELDPNEGPGLGCFTVQAILFAAILLLIPIGVFSLGWP
                     VWFLTALLVLAIILLFFISMTSVFLLRVFFASRRTGRGRTYGATAAPMTIDTEEPEPA
                     NPSTTESEE"
     gene            complement(1341..2744)
                     /locus_tag="VGO32_06050"
     CDS             complement(1341..2744)
                     /locus_tag="VGO32_06050"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV7200348.1"
                     /translation="MNPRARFGSLDDPPASFVLRGVRIVDPENSTSVIGDLSVVDGVI
                     REELAPFDVPRVDAMGLVAAPGFCDLHAHLRTPGGETAETIESGTRAAAHGGYTTVCV
                     MPNTDPPLDDPWRLRGVTAAAANAAARVRVVAAATRGREGAELTDHAALTEAGAVAVS
                     DDGATIGSAAVVRRVLSYLGTVGRPLIEHAEEPTLAGSAVMRAGATATRLGLEGWPAS
                     AELAVVERDLTLAAETGGWIHFTHLSTAAAAARIRTARAAGIRVTCDVTPHHLALTDA
                     WVAGDRRFSWDDPDGAPDVLDASLAYDGRCRVNPPLGTRDDALALLAGVADGTIDAIA
                     TDHAPHPPERKAVPFAEASPGLVGLETALGIGLMAVAVGQLPLTTLIGALSNRPAGII
                     GERRSLADGSVADLVIFDPTRAWTVQPDALASRSANTPLLGMDLPGVVRLTVADGRVT
                     YDDGLIGPADAIRVEAR"
     gene            complement(2755..>3328)
                     /locus_tag="VGO32_06055"
     CDS             complement(2755..>3328)
                     /locus_tag="VGO32_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEV7200349.1"
                     /translation="AARSLERGTVVNGGDGTHAHPTQALLDALTLREALGTLDGARVV
                     IVGDAAHSRVARSNLHALVRLGASVVLCGPPAWVGMFGEWPGVTVVTDLHQALVGADA
                     VMALRVQLERAAGRGVPSLDEYVSRWGLTVDRLAKACPQAWLMHPGPANEGVEISAEL
                     STAPRSLVGRQVENGVPVRMAVLSLLAADR"
BASE COUNT          497 a         1164 c         1185 g          482 t
ORIGIN      
        1 gtcgtctcga accagccgtc gcctcgcatg acgagctggt catcggactc gtcgagcagg
       61 acgaggtctc cggtcaggcg cagggcatcc tccgcgttgc ccacgaactc gcgcaggagc
      121 gccgcctgct gctcctggag ctgggagcgg taggcatcgg ccagctgcca gagccgctga
      181 gcggcgaggg tctcgcgctg gtcggccggg agctcgttct gccagttgtc gtcctcggtc
      241 tcgggcggcg gcgcctcctc ggcggccagc tcattggcgg gggtgaggtg gtcgatcggg
      301 tttcgggatg cctcgatcag ccaggcgagg atatcggccg ggttgtagag ctcgatcagc
      361 tccgacaggg tgtcgacctc ctgctcctgg tcgtaccact ccccgtcggc cggatcctgg
      421 tatgtcaggc gcgtgaccca tagctggtca atcgggtgga agcggagaaa gtcctgctcg
      481 tcgtccacca gcgtgatcgg gccgatctcc gtgagccgct cgcggttggc gtccaggaac
      541 cgtcgcagct gctcggtcgc ctcggacacg aagtcgcgcc gctcgagctc cgcctcctcg
      601 gccagctcgc ggagcgcgac cagccgcggc gagctcacgg cttgtacacc aggtccggac
      661 ccatccgctc ccggaggaag atcgtgtgct tggtgtcgaa gatcgactcg gccacgttgt
      721 ggcccagtcg ccgagtggcg gggtcctggt acagctggtc gaggacctcg tagttcggga
      781 ggtcgaagat gatcacgttg ttccactccg acccctcggt cacgttgaac cagccgatga
      841 acttgatccc gtacttctcc tcgtagtcgc ggacccggct cgggaacatg tggcgcatgc
      901 cgcgcttgaa catcgtgtcg cgcagcacgc cgtgacggac gttgaagaag gtgaccatca
      961 gcggcacggt gctattcctc gctctcggtg gtcgatgggt tggccggctc gggctcctcg
     1021 gtgtcgatgg tcatcggcgc cgcggtcgct ccatatgttc gaccacggcc cgttcgtcgc
     1081 gaggcgaaga acacgcggag caggaagacc gaggtcatgg agatgaagaa cagcaggatg
     1141 atggccagca ccaggagcgc ggtcaggaac cagaccggcc acccgagcga gaacaccccg
     1201 atcgggatga gcagcaggat ggccgcgaac aggatggcct gaacggtgaa gcaccccagg
     1261 ccggggccct cgttggggtc gagctcctcg ttcgtgcgga tggccatgcg gccccgcatc
     1321 gtgccacaag ccggtgacgg tcaccgggcc tccacccgaa tcgcgtccgc cggaccgatg
     1381 agcccatcgt cgtaggtcac gcgcccgtcg gccaccgtga gccggacgac ccccggcaga
     1441 tccatgccca gcagcggcgt gttcgccgat cggctggcca gcgcgtcggg ctgcaccgtc
     1501 cacgcccggg tggggtcgaa gatgacgagg tcggccaccg aaccgtcggc caacgaccga
     1561 cgttcgccga tgatccctgc cgggcgattg gacagggcgc cgatcaaggt ggtcagcggc
     1621 agttgcccga cagcgaccgc catcaagccg atgccgagcg cggtctccag accgacgagc
     1681 ccgggcgatg cctcggcgaa cggaaccgcc ttccgctcgg gcggatgcgg ggcgtggtcg
     1741 gtggcgatgg cgtcgatagt gccgtccgcg accccggcga ggagcgcaag ggcgtcgtcg
     1801 cgggtcccga gcggcgggtt cacccgacag cggccgtcgt acgcgagtga cgcgtcgagc
     1861 acgtcgggtg cgccgtccgg gtcgtcccac gagaagcggc ggtcgcccgc gacccacgca
     1921 tcggtgaggg cgaggtggtg tggcgtcacg tcgcacgtga cccggatgcc ggccgcgcga
     1981 gccgtgcgga tccgggcggc cgccgccgcg gtcgacagat gggtgaagtg gatccagccg
     2041 ccggtttccg cggcgagagt caggtctcgc tccacgaccg ccagctcggc gctcgccggc
     2101 cacccctcca ggcccagccg ggtggccgtg gcgccggccc gcatgacggc gctcccggcc
     2161 agggtcggct cctcggcatg ctctatcaac ggccgcccca ccgtgccgag atatgagaga
     2221 acgcggcgga ccacggccgc gctgccgatc gtcgcgccgt cgtcgctgac ggccacggcc
     2281 ccggcctcgg tcagagccgc atggtcggtc agctcggcgc cctcccgccc gcgggtcgcg
     2341 gcggcgacca cccgcacccg ggcggcggca ttcgccgccg cagccgttac gccgcgcagg
     2401 cgccaggggt cgtcgagtgg cgggtcggtg ttcggcatca cgcaaacggt tgtgtaccca
     2461 ccgtgggcag cggcccgcgt gccggactcg atcgtctcgg cggtctcccc gccgggcgtc
     2521 cgcaggtggg catggaggtc acagaacccc ggcgcggcga ccaggcccat ggcatcgacc
     2581 cggggcacgt cgaatggagc cagctcctcg cgaatgaccc cgtccacgac cgacaggtcg
     2641 ccgatcacgc tcgtgctgtt ctcgggatcg acgatccgta ccccgcgcag tacgaagctg
     2701 gcgggcggat cgtccagcga gccgaatcgg gcgcgggggt tcacacgccc tccgtcatcg
     2761 gtctgccgcc agtagcgaga ggaccgccat ccggaccggc acgccgttct cgacctgccg
     2821 gccgacgagc gaacgcggtg cggtcgacag ctcggcgctg atctcgacgc cctcgttggc
     2881 cggtcccgga tgcatgagcc aggcctgcgg gcaggctttc gccagccgat cgacggtgag
     2941 gccccagcgg ctcacgtact cgtcgagcga cgggaccccg cgacctgcgg cgcgctcgag
     3001 ctggacgcgg agagccatga ccgcatcggc tccgaccagg gcctggtgga ggtcggtcac
     3061 gaccgtcacg ccgggccact ccccgaacat gccgacccag gccggtggcc cgcacagcac
     3121 gaccgacgcg cccaggcgca cgagcgcatg aagattcgag cgcgccaccc gcgagtgggc
     3181 ggcgtcgccc acgatcacga cccgcgcgcc atccagcgtc ccaagcgcct cgcgcagggt
     3241 gagggcgtcg agcagggcct gggtgggatg cgcatgcgtg ccatcgccac cgttcaccac
     3301 cgtgccgcgc tcgagggatc gggcggcg
//
LOCUS       DASYGU010000216         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1577790, whole genome shotgun sequence.
ACCESSION   DASYGU010000216 DASYGU010000000
VERSION     DASYGU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577790"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VGO32_06060"
     CDS             complement(<1..1001)
                     /locus_tag="VGO32_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200350.1"
                     /translation="MTDTHDLLEEWLVDGAVGEPPREAAVHASTCESCARRLGAFDSL
                     AAVNVGAAGSPPPAILAGRFSAVVARTRLATTVTGTILAGILVVYGASQLVGFVGGPP
                     GPPPTDAAGLFSVAQDGAPTDTLGPQPADQPTPADEPPGTPITPSIPLPSIAPNEAPP
                     ASPNAPFVSRSVVGYYTAGIRWSASASGGPAFKWELWRRAGSGSWIKLGELAGSVQSF
                     TNGGLSPGSTYGYRLRAVNVSGASGFSNVLTITTLVLPTAAPTPTESPTPTPAPVDCN
                     DGVDNDGDALVDYPEDPGCSSGTDPSESPYNGQCNDGVDNDGDGFVDYPDDPRCSSGT
                     "
     gene            complement(998..1537)
                     /locus_tag="VGO32_06065"
     CDS             complement(998..1537)
                     /locus_tag="VGO32_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200351.1"
                     /translation="MDDHILDQDFVALHGDRLLGFAMLVTLGDASLAGKLSAEALGRG
                     VERIYQLRHPVRAATWLRAAVTKAAEGSSWGHQRPSEIERRDALRPLGVNPAMFDALI
                     SLDVRDRAAVVATAVEGFAGADVHEIVGSDARVRRARRDYLTAYVAAAETRHTPPAAG
                     ELAARVEAAAAPILSGSTK"
     gene            1697..3514
                     /gene="dnaG"
                     /locus_tag="VGO32_06070"
     CDS             1697..3514
                     /gene="dnaG"
                     /locus_tag="VGO32_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV7200352.1"
                     /translation="MSSAGSVADIKSRLPVAEIVGETVNLKKAGSTWKGLCPFHGEKT
                     PSFTVSPERESWHCFGCGEHGDIFTFVMKRDGIDFREALGRLAERAGVELSAAGARED
                     RRRKRLRDALDAAIAWYREVLRQAHQAEPARRYLAERGFTEETIDRFGIGFAPNSWDA
                     LVKRLAARGFSSEEMVAAGLASMGQRGRPVDRFRGRIMIPIRDASGKATGLGGRVMPD
                     GEGPKYLNSPATALFDKSRTLYGIDLAKTSIRRDKLAVIVEGYTDVMAAHQAGFTNVV
                     ASMGTALTSGQVDIVTRYADAITLAYDVDLAGEAATERGLLEELGPSQSITKLRVIRI
                     PAGKDPAELLQTDPSAWREAIENAKPLIDYFMEREAARVDMNSVGGRREMAGRVLAKL
                     QRVTDPVVQDLYVQELSKIVQVDERILREGLARPRVQRRAASVHSEPVGPGAPALDAV
                     ETETLALLIRHPGLTVDLASNPLPLGRPEASSLAEAWAAWVAQSTSSPVDVEGFVATL
                     DPISADLARVVIHAAGSAPSLEPAMAAEALRVCRLRLRRARIEEAIGDGRHLLEDAER
                     DGDRELLEQIETHITKLGREKAEVTKLLTTPAVPVRGGS"
     gene            3829..4340
                     /locus_tag="VGO32_06075"
                     /pseudo
     CDS             3829..4340
                     /locus_tag="VGO32_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            4927..5330
                     /locus_tag="VGO32_06080"
                     /pseudo
     CDS             4927..5330
                     /locus_tag="VGO32_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879897.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          949 a         1778 c         1847 g          756 t
ORIGIN      
        1 gtcccagacg agcacctcgg gtcgtctgga tagtcgacga agccatcccc gtcgttgtcg
       61 accccatcgt tgcattggcc gttgtaggga gactcggacg gatctgtccc agacgagcag
      121 cccggatcct ctggatagtc aacgagggca tcaccgtcgt tgtcgacccc atcgttgcag
      181 tcaaccgggg ctggggttgg cgtgggcgat tccgttggtg tcggagcggc cgtcgggagg
      241 accagtgtcg tgatcgtgag aacgttggag aacccactgg cgccactgac gtttacggcc
      301 cggaggcgat aaccgtacgt cgagcctggg ctgagaccgc cgttggtgaa ggactgcacg
      361 gagccggcga gctcgccgag cttgatccac gatccggacc cggcccggcg ccagagctcc
      421 cacttgaacg ccggtccgcc actggcactc gcactccatc gaatccccgc ggtgtagtac
      481 ccgaccacac tccgggagac gaatggggcg ttgggcgacg cgggaggcgc ttcgttcggc
      541 gcgatggacg gcagggggat ggatggcgtg attggggtgc ccggtggctc gtcggctggg
      601 gtcggctggt cggcgggctg tgggccgagg gtatcggtcg gggcgccatc ctgggccacc
      661 gagaacaagc ctgcggcatc cgttggtggg ggacccggag gtccccccac gaagcccacg
      721 agctgcgagg caccgtagac cacgaggatg ccggccagaa tcgtgccggt cacggtggtc
      781 gcgagccggg tccgggcgac cacggccgag aagcggccgg cgaggatggc gggtggggga
      841 gatccggctg ccccgacgtt gacggcggcc agcgagtcga acgctccgag ccgacgagcg
      901 cacgattcgc aggtggacgc gtggacggcc gcctctcgcg gcggttcgcc gaccgctccg
      961 tcgacgagcc attcctcgag caggtcgtgg gtatcggtca tttggtcgag ccagacagaa
     1021 ttggagcggc cgctgcctcc acgcgtgccg cgagttctcc cgccgcagga ggcgtgtgac
     1081 gtgtctcggc cgcagccacg taggcggtca ggtagtcgcg acgtgcacgt cgcacccggg
     1141 cgtcgctgcc cacgatctcg tgcacgtccg ccccggcgaa gccttcgacc gcggtggcca
     1201 cgactgctgc gcggtcacga acgtcgaggc tgatcagcgc gtcgaacatg gcggggttga
     1261 cgcccagtgg tcgcagcgca tcccgtcgct cgatttcgct cggacgctga tgaccccacg
     1321 acgagccctc ggcggccttg gtcaccgcgg cccgcagcca ggtggcggcc cgaaccggat
     1381 ggcgaagctg atagatgcgc tcgacgcccc gcccgagggc ctcggcagac agctttccgg
     1441 cgagcgaggc atcgcccagg gtcacgagca tcgcgaatcc gagcagccgg tccccgtgca
     1501 gcgcgacgaa atcctggtcc aggatgtggt cgtccactga cggatggtag cccaacgccg
     1561 gttgaacctc agtcaccccc ggaggggtac aaaagacgag tgaatacttg acaatgggtt
     1621 agaatgcctc tggactcttg acagaacact tgacaaatcc cttgacacgc gccgatcagg
     1681 cgctcgggac ttccccatga gctcagccgg ctccgtcgcc gacatcaagt cgcggctgcc
     1741 cgtcgccgag atcgtcggcg agacggtcaa cctcaagaaa gctggatcga cctggaaggg
     1801 cctgtgcccg ttccacgggg agaagacacc ctcgttcacg gtcagccccg aacgcgagtc
     1861 gtggcattgc ttcggctgtg gcgaacacgg cgacatcttc accttcgtca tgaagcgcga
     1921 cggaatcgac ttccgcgagg ccctggggcg gcttgccgag cgcgcgggcg tggagctgtc
     1981 cgcggcagga gcccgagagg atcggcgtcg aaagcgcctc cgcgacgcgc tggatgcggc
     2041 gatcgcctgg tatcgcgagg tgctccgtca ggcgcaccag gccgagcccg cccgtcggta
     2101 tctcgccgag cgagggttca ccgaagagac catcgatcgg ttcggtatcg gctttgcccc
     2161 caacagctgg gacgcgctcg tcaagcggct ggccgcccgc ggcttttcat cggaggagat
     2221 ggtggccgcc ggactggcga gcatgggcca gcgcggacga ccggtcgacc gattccgcgg
     2281 ccggatcatg attcccatcc gcgatgcctc cgggaaggcc accggactgg gtggtcgggt
     2341 gatgcccgac ggggaaggac ccaagtacct gaactcgccg gcgacggcgc tgttcgacaa
     2401 gagccgaacg ctctacggca tcgacctcgc caagacttcc atccgccgcg acaagctggc
     2461 cgtgatcgtc gagggctaca ccgatgtgat ggccgcccac caggcgggct tcacgaacgt
     2521 cgtcgcctcc atggggaccg cgctgaccag cggccaggtg gacatcgtca cccgctacgc
     2581 ggatgccatc accctggcct acgacgtcga cctggcaggc gaagccgcaa cggagcgcgg
     2641 gctgctcgag gagctcggtc cgagccagag catcacgaag ctgcgggtca tccgcatccc
     2701 cgccggcaag gatcccgccg agctgctcca gaccgatccg agcgcgtggc gcgaggcgat
     2761 cgagaacgcg aagccgctga tcgactactt catggaacgc gaagcggcgc gcgtggacat
     2821 gaactcggtc gggggtcgtc gcgagatggc cggtcgggtc ctcgccaagc ttcagcgggt
     2881 caccgacccg gtcgtccagg atctctacgt tcaggagctc tcgaagatcg tgcaggtcga
     2941 cgagcggatc ctgcgcgagg gcctggcccg gccgcgggtg cagcgacgcg ccgcgagcgt
     3001 tcattcggag ccggtcggac ccggggctcc ggcactggat gcggtcgaga ccgaaacgct
     3061 ggcgctgctc atccggcatc ccggcctgac cgttgacctg gcctccaatc cgctgccctt
     3121 ggggcgcccc gaggcgtcgt cactggcaga ggcctgggcg gcgtgggtgg cacagtccac
     3181 gagctccccg gtcgacgtgg agggcttcgt ggccacgctc gatccgatct ccgcggacct
     3241 ggcgagggta gtgatacatg ccgcgggttc ggcaccgagc ctcgagccgg ccatggcggc
     3301 ggaggcactg cgggtttgcc gccttcgact tcgccgggcg cggatcgagg aggcgatcgg
     3361 cgatggccga cacctgctcg aggatgcgga gcgcgatggc gaccgcgagc tgctggaaca
     3421 gattgaaacg cacatcacca agctcggccg agaaaaggcc gaggtcacca agctgctgac
     3481 gacaccggcg gtgccggtgc gaggaggatc ctgaccgtgg cgttgaagac cccgacccgt
     3541 gaacccgtga aggcggcgtc caaggcgccc accaagagtc cgtctgtgaa gcccacggcc
     3601 gccaagccgg ccaccgcaaa ggcgacggcc accacgaagg ccgcggctgg gaagccggcc
     3661 ggcaaggccg cggttgcgaa ggcgtccgcc gtgaagaccg cggcaccgaa ggccgcagcg
     3721 gccagcaacg ccgcgcccaa ggcggcgcca aagacaggcg ctgtgaagac gccggccgcg
     3781 aaggtcgccg ccaagcccgt gaccgcggcc aaggcgactc ccacgaaggt ggccacgacc
     3841 gataagcagc caaccaaggc agccgccaag gctcccgcag ttgccaagcc ggtccttgcc
     3901 gcctcaccag ccgccaagcc cgccgcaaag ccagcaaagc cgagccgcaa gaaggccgct
     3961 cccttgacct tcaccgacgc gtcgcctgag gcggcgtccg cggtcgagga tctcctgtcc
     4021 cacggtcggg aagacgggtt catcacccat gaccagatcc tggccgcggt acccagcccc
     4081 gaggccaaca tggatgccgt cgaggagctg ttcgccggtg ccgaggaaca gggcgtcgag
     4141 gtcttcgatt cggagaacca gcccacctgg agcgagccat ctgacgacgt cgcggtttcg
     4201 cttcccggcg aggatgccgc tgacaaggcc gagaaggagc ccgaggagga cctggaggcg
     4261 ctggccgccg acctgatcgg gatcgacgac ccggtccgga tgtacctcaa ggagatcggc
     4321 aaggtcgcac tcctgaccgc cgaggaagaa gtcgtcctgg ccaaggcgat cgagctcggc
     4381 gagctcgcga tcgaggatcc cgggcgggcg atgaccaacc tctacacctg ggtcaccctc
     4441 aactccgaac ccaaggcccg ggccatggcc gccatgcggg cgttcgacct gccgcaggac
     4501 tcaccccacg tcacgctcga cgcaatcgac tggtggatca agcgcaagcg gaacacgatc
     4561 gagcgtccga acatccggtt cacgaaggca cgccgcgacc ccgagctcga tgacgaggga
     4621 cggcagcgga tcatggaggc cgagtcactc ctgaagtcgg tcgccaccga acccgcccag
     4681 gcgctcaagg atgcggtcct gttcggtcac tcctatcggt tccgggtgct cgagcagccg
     4741 ggcaaggagg agctgtacgg cctcgagcgc tgggcccgcg agacggcgca gggcatcacc
     4801 cgtgcctaca tcgaggccgg caacgacgcc gactacatga agtcgatggg ctacgacccg
     4861 tctgtcccgc ccgaggtgcc gctcgagcgc cgatccggac gcctggtcga gcagagcacc
     4921 gatggccgac gccggctgac cgaggccaac ctccggctgg tggtgagcat cgcgaagaag
     4981 tacatcgggc gcgggatgag cttcctggac ctcatccagg aggggaacat cggcctcatc
     5041 cgggccgtgg agaagttcga gtacgagaag ggcttcaagt tctcgaccta cgccacgtgg
     5101 tggatccggc aggcgatcac ccgggccatc gccgaccagg cgcgcacgat tcggatcccg
     5161 gtccacatgg tggagaccat caaccgcctg atccgagtca gccggaccct gctccaggag
     5221 ctcggtcgcg agcccacggt cgaggagatc gccgaggcga tgtcgcgcgg ccaggaggtc
     5281 gtggtcaccc cggacaaggt ccgcgagatc atcaaggtct cgcaggagcc
//
LOCUS       DASYGU010000217         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_549257, whole genome shotgun sequence.
ACCESSION   DASYGU010000217 DASYGU010000000
VERSION     DASYGU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549257"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VGO32_06085"
     CDS             complement(<1..340)
                     /locus_tag="VGO32_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV7200353.1"
                     /translation="MRVVAAPKDMVWVPGGTFRMGSDDWYPEERPVHSVAVDGFWMDE
                     HPVTVAEFRRFARATGYVTRAERPLDPALYPGADPALLVPGALVFRKTRGPVDLRDVR
                     NWWAYVPGARW"
     gene            complement(618..1724)
                     /locus_tag="VGO32_06090"
     CDS             complement(618..1724)
                     /locus_tag="VGO32_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV7200354.1"
                     /translation="MSERTRFVGLDVHKASISAAVAGPYGEPEDHGVIANDAASVRRL
                     LQRLGDDGHRLAVAYEAGPTGYALHRQVRAMGIECIVVAPSLIPVRPGERVKTDRRDA
                     AKLARLLRSGDLVAVWVPDEAGEALRDLVRARDDAKADQLRARHRLSKFLLRRGIQPT
                     AGVRAWSRAHEAWLGRLTFERTADAVVLGDYLAVARAASERVKRLEGALLQVAAEGPQ
                     AELVAALQAIRGIGFLSAVTIAAEAGDLRRFATARQFMAYVGPVPSEWSSGPARHRGR
                     ITKTGNRLIRHVLGQAAHNARHRPNVSSGLRARQRLVIPEIVDLDRKAQARLHHRYRS
                     LVGRIGVNKTVIAVARELAGFVWAAGQHVAPRAA"
BASE COUNT          259 a          679 c          717 g          285 t
ORIGIN      
        1 cccaacgcgc ccccgggacg taggcccacc agttgcggac gtcgcgcagg tcgacaggac
       61 cgcgcgtctt gcggaacacg agcgccccgg gcaccagcag ggccgggtcg gcgcccgggt
      121 acagggcggg gtcaagcggg cgctccgcgc gcgtgacgta cccggtggcc ctcgcgaagc
      181 ggcggaactc ggccacggtg acggggtgct cgtccatcca gaagccgtcg acggccacgc
      241 tgtggacggg ccgctcctcc gggtaccagt cgtccgagcc catccggaac gtgccgcccg
      301 gcacccagac catgtccttc ggcgcggcga ccactcgcat ccgcggatag tcgcacgctt
      361 gcctacgaca cgtcatgaca ctgatatgga aggatttgtc aaggggtagg tggcggggag
      421 tggcggggca tggccggggt caggtcctgg cgatcgtcgg tcactctgat atgcgcgggt
      481 cggcgaggcc gttgccgcat gacagtgagc cgatcatcga ggtgcctcgg actagtggcg
      541 cgagtcggga tcgcatagtg tcctcgaggg tcctcgatgc tgctgtcccg ttcggccatg
      601 ccccgcgacg ccatgtgtca ggctgccctc ggtgccacgt gttgcccggc tgcccagacg
      661 aagccggcca gctcgcgcgc cacggcgatg acggtcttgt tcaccccgat ccggcccacc
      721 aggctgcggt agcggtgatg gagtcgtgct tgggccttgc ggtcgaggtc cacgatctcg
      781 gggatgacga gacgctgtcg ggcccgcaga cccgagctca cgttgggtcg gtggcgggcg
      841 ttgtgggcgg cctggccgag gacgtggcgg atgagccggt tgccggtctt ggtgatgcgg
      901 ccccggtgac gggcggggcc gctcgaccac tcggagggca ccgggcccac gtaggccatg
      961 aactggcgcg ccgtggcgaa gcggcgcagg tcgcccgcct ccgcggcgat ggtcaccgcc
     1021 gacaggaagc cgatgccccg gatcgcctgg agggccgcga cgagctcggc ctggggaccc
     1081 tcggcggcga cctgcaggag cgcgccctcg aggcgcttga cccgttcgga cgcggcccgg
     1141 gccaccgcga ggtagtcgcc gagcacgacc gcgtcggccg tccgctcgaa ggtcagccgg
     1201 ccgagccagg cctcgtgcgc ccggctccag gcgcgcacgc cggcggtggg ctggatgccc
     1261 cggcgcagca ggaacttgga gagccgatgg cgggcacgga gctggtcggc cttggcgtcg
     1321 tccctcgccc ggaccaggtc gcgcagcgcc tccccggcct cgtcgggcac ccacaccgcg
     1381 accaggtcgc cgctgcgcag gaggcgcgcc agcttggccg catcgcgccg gtcggtcttg
     1441 acgcgctcgc ccggccgcac cgggatgagc gagggcgcga cgacgatgca ctcgatgccc
     1501 atcgcgcgca cctggcggtg gagcgcgtag ccggtcgggc ccgcctcgta ggccacggcc
     1561 agccgatggc cgtcgtcgcc gagccgctgc agcaggcgcc gcaccgacgc ggcatcgttg
     1621 gcgatgaccc catggtcctc cggctccccg tagggaccgg ccacggccgc cgagatcgac
     1681 gccttgtgga catccagccc gacgaagcgg gtacgctcag acatggaccg gcctcccgta
     1741 tgtggctctg tgcgggcaag ctcaggcgac ccgcgcaatc cacgattgtg cgcgagtgcc
     1801 ggtccttcca tgttgactag acccgatggc gttgctcgga tcgcggctga tgcttcggca
     1861 tgcgccctcc ggcttccctc cggagccgtc gcgctccgtg tgggatctct gtcgttgtgc
     1921 gtcggcaggt gccgtagcat
//
LOCUS       DASYGU010000218         6710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1474951, whole genome shotgun sequence.
ACCESSION   DASYGU010000218 DASYGU010000000
VERSION     DASYGU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474951"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VGO32_06095"
     CDS             complement(<1..1591)
                     /locus_tag="VGO32_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7200355.1"
                     /translation="MTFDRVAVVEDALDALLAGLAPASDPLGPDEPIRSGSDLTARRA
                     VELFEDQVVSRQIDRAARELKKTNRSFHTIGGAGHENNAVLGAQLRITDPGFLHYRSG
                     GFMMARARQLPGNTPAFDALLGVVASREDPIAQGRHKVWGSRSLWIPPQTSTIASHLP
                     KAVGMAYALARARRLGLADELPPDAIVMCSFGDATVNHATALSAFNTARYGARIGLPM
                     PILFVCEDNQTGISVPTPEGWIESTFAAQPYLHYRHASGELDEVWDTVAEAIHVVRAA
                     REPLFLHLDVVRLWGHAGSDAEHGYRSADEIAATERADPLLRNAERLVALGAATPDAL
                     RALLADARERVWAAAEEAARRPRLSTTEEVVAPLAPDHPDRVAVRVAEPPPDPDLRAV
                     AFGATLPENATQPTARTLAGHINAALTDELARRPEIVVFGEDVGRKGGVYNVTHDLQK
                     RFGAGRVFDTLLDETSILGIAQGAAHAGLLPIPEIQYLAYIHNALDQIRGEAASLQFF
                     SNGQFRNPMVVRVPGLAYQKGF"
     gene            complement(1824..2477)
                     /locus_tag="VGO32_06100"
     CDS             complement(1824..2477)
                     /locus_tag="VGO32_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV7200356.1"
                     /translation="MTEDVFALIEAGDVDGLRELLARDPAAAGARREDGTSAILVARY
                     QFKLDAVAALLAAGPPLDIFDAAAVGQTEVVRSELARDPGLAVAFARDGFTALGLACF
                     FGHPDAAKALIDGGARVNDASRNPMKVAPLHSALAGGHAEITELLLVAGADVNAVQAD
                     GYTPLHEAAQNGDQTLAERLVTVGADPRARLDDGSTPADSARKAGHVALADWLKSRA"
     gene            complement(2474..3322)
                     /locus_tag="VGO32_06105"
     CDS             complement(2474..3322)
                     /locus_tag="VGO32_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7200357.1"
                     /translation="MIQPGVLWGLATAAAWGTADFTGGLASRRVAPFAVTAGSQVIGL
                     VALVVGVLLLAPPIPAADTLLLGAVAGLGGGLGLAFLYEGLARGSMGIVSSLSGAGAA
                     VIPLLATLLLGTQIAPIALVGVALIVAAGLAAADISRDAASRSALLLALGAAIGFSSW
                     YLIIDRAAADGGIWALVASRTASVLLIGGIVAATKARASVRSLPSVLPLVVLAGVLDV
                     GANVFFVVARGEISVALAATLSGLYPLATMLLARVVLGERLPRLGLLAVLLAVTGTIL
                     ISVSRA"
     gene            3452..4567
                     /locus_tag="VGO32_06110"
     CDS             3452..4567
                     /locus_tag="VGO32_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200358.1"
                     /translation="MAIEPAAPEVASAPGAAAPAVVLENLERTYGDVVAVAGINLSIA
                     DGEFFSMLGPSGSGKTTTLRMIAGFEIPTAGRVLLHGQDVSTIPPFDRDVNTVFQDYA
                     LFPHMTVGDNVAYGLEIRKVARAERERRVGEAMAMVRLEGYEKRRPGQLSGGQRQRVA
                     LARALVNRPRVLLLDEPLGALDLKLREQMQIELKEIQKEVGITFIYVTHDQEEALTMS
                     DRLAVFNRGLIEQVGTPADVYEHPKTPFVAGFVGTSNLLKDEAARVVIGQAGTFTVRP
                     EKIRLAAPEDAVASDEHSALGKVSEVVYLGSDTRYIVALDAGGELVVTQQNLQTSSME
                     ALAAQGRPVRLIWRRQHVLSLADGQESAGEIEGVERP"
     gene            4564..5724
                     /locus_tag="VGO32_06115"
     CDS             4564..5724
                     /locus_tag="VGO32_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7200359.1"
                     /translation="MRLLRIPVMLTVFGLLAAACSGGAPSGALTELPDAEGALNLVIW
                     AGYAEDGTNDPNYDWVNPFVEETGCEVNTTDMTDSANGVELLQSGQYDGGSFSGDATL
                     RLIAGEDVAPINTELIPYYADVFEGLKGQPHNTVDGVPYGTPHGRGANLLLYNTDTFT
                     EAPTSWDPIWDADSPAAGSVSVYNSAIFIADAALRLKTTNDNEFGIDDPYQLTEEQFN
                     AAVELLTEQHAIVGEYWNYATDQITSFGAGDMLAGTSWQYQANNLPDAPVGIVKPAEG
                     TTGWSDTWMIASEAEHPGCMYRWMDWMLSPEANAQATVWFGEAPVTQAACDRAEELSP
                     NHCEDYHATDEAYFEDVAFWSTPREDCNDEDDATTCKTIEDWIQAWTEITGS"
     gene            5820..>6710
                     /locus_tag="VGO32_06120"
     CDS             5820..>6710
                     /locus_tag="VGO32_06120"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200360.1"
                     /translation="MTSSTMSPSPSRGRRFATWLSGRPRLQLALLLALPAGFFVIFYL
                     GSLLLLFLNAFWETDPFSGDVIPVFSLDAFVTIFTVDVYRIIAARTLGMAIVVTLTCV
                     ALGFPVAYYMARIASPRTRRLMVIAVLMPLWASYLVKIYTWRTMLGGNGFVDWLLAPF
                     GLEGPGLNSLVGSWLVLTYLWLPYMILPIQAGLERIPGSLLEASADLGARSGRTFRRV
                     VIPLVLPSIAAGSIFTFSLTLGDYITATMVSSTKFIGNVIYDNSALGSLPLAAAYSFV
                     PIVIMIVYLVVARRLGAFESL"
BASE COUNT         1055 a         2323 c         2320 g         1012 t
ORIGIN      
        1 cgaatccctt ctggtaggca agcccgggca ctcggacgac catcgggttg cggaactggc
       61 cattgctgaa gaactggagc gacgccgcct cgccccggat ctggtccagc gcgttgtgga
      121 tataggcgag gtactggatc tccggaatgg ggagcaggcc ggcgtgggcg gcgccctgcg
      181 cgatgcccag gatcgaggtc tcgtccagca gcgtgtcgaa cacccgtccc gccccgaacc
      241 gcttctggag gtcgtgggtc acgttgtaga ccccgccctt gcggccgacg tcctcgccga
      301 agaccacgat ctccggccgc cgggcgagct cgtcggtcag cgcggcgttg atgtgtccgg
      361 ccagcgtccg ggcggtgggc tgggtcgcgt tctcgggcaa cgtggcgccg aacgcgacgg
      421 cccgcaggtc ggggtcggga ggcggctccg cgacgcggac cgcgacccga tccggatggt
      481 ccggggccaa tggtgcgacg acttcttcgg tcgtgctcag gcggggccgg cgggcggcct
      541 cctcggcggc ggcccagacc cgctcgcgcg catcggcgag gagcgctcgg agcgcgtccg
      601 gcgtcgcggc ccccagcgcc accagccgct ccgcgttgcg caggagcggg tcggcgcgtt
      661 cagtggccgc gatctcatcg gcggaccggt agccgtgctc cgcgtcggat ccggcatgac
      721 cccacaggcg cacgacgtcg aggtgcagga acaagggctc gcgggcggcc cgcacgacat
      781 gaatcgcctc ggcgacggtg tcccacacct cgtccagctc gcccgacgcg tgacggtaat
      841 ggaggtacgg ctgggcggcg aacgtggact cgatccaacc ttcgggggtg ggcacgctga
      901 tcccggtctg gttgtcctcg cagacgaaca ggatgggcat gggcagaccg atgcgcgcgc
      961 cgtagcgcgc ggtgttgaac gcggacagcg cggtcgcgtg gttcacagtc gcgtcgccga
     1021 acgagcacat cacgatggcg tccggcggga gctcgtcggc cagtcccagc cgcctcgcgc
     1081 gggccagggc gtaggccatg cccaccgcct ttggcaggtg cgacgcgatg gtcgaggtct
     1141 ggggcgggat ccacagcgag cggctgcccc agaccttgtg ccggccctgg gcgatggggt
     1201 cctcgcggga cgcgaccacg ccgagcagcg cgtcgaacgc gggcgtgttg ccgggcagct
     1261 ggcgagcgcg ggccatcatg aatccgcccg atcggtagtg gaggaatccc gggtcggtga
     1321 tccgcagctg agcgcccagg accgcgttgt tctcgtggcc ggccccgccg atggtatgga
     1381 agctgcggtt cgtcttcttc agctcgcggg ctgcccgatc gatctggcgg ctgacgacct
     1441 gatcctcgaa cagctcgacc gcgcggcggg cagtcagatc cgatccggat cgaatcggct
     1501 cgtccggacc gagcgggtct gatgcgggtg ccaggccggc gagcagcgcg tccaacgcat
     1561 cctcgacgac ggcgacccga tcgaaggtca tgcggaggcg acctctcggg cagctgggtc
     1621 ccggccatca tagccgcggt tccgcgccca ctccttgccg gcctcggacc ggctgtcatc
     1681 aggccggcca gccgccgccg gtggtggccc gggtcgggtt ggacatcggt ctgctcctcg
     1741 tagaggccga gaccggcgtg ccggcgattg cccgcggctc gtctcgtcgg gaccgatgaa
     1801 agcgtcgggg cgttaccgcc ccattacgcg cggcttttca gccagtcggc aagggcgaca
     1861 tgccctgctt tgcgggcgga gtcggccggg gtcgagccgt catccagtcg agcccgggga
     1921 tcggccccga ccgtgacgag ccgttcggcg agggtctgat cgccgttctg cgccgcctcg
     1981 tggagcgggg tgtagccgtc ggcctggacc gcgttgacat cggctccggc gaccagcagg
     2041 agctcagtga tctcggcgtg gccgccagca agggcgctgt gcagaggagc gaccttcatc
     2101 gggttgcgag aggcgtcatt gacccgcgca ccgccgtcga tcagcgcctt cgccgcgtcc
     2161 gggtggccga aaaagcatgc caggcccagc gcggtgaacc cgtcgcgggc aaaggccacc
     2221 gcgagcccgg gatcgcgcgc gagctccgac cggacgactt cggtctggcc cacggccgcg
     2281 gcgtcgaaga tgtccagtgg gggaccggcg gccagcaggg cggcgaccgc gtccagcttg
     2341 aactggtatc gggccacgag gatggcgctc gtcccgtcct cgcgacgcgc tccggctgcc
     2401 gccggatcgc gcgccaggag ctcgcgcagg ccgtccacgt ccccggcttc gatcagcgcg
     2461 aacacgtcct cggtcacgcg cggctgaccg agatcaagat cgtgccggtc acggcgagga
     2521 ggacggccag cagcccgaga cgggggagcc gctcgccgag gacgacgcgg gccagcagca
     2581 tggtcgcgag agggtacagc ccggacaggg tggcggcgag ggcgacgctg atctcgcccc
     2641 gggccacgac gaagaacacg ttggccccga catcgagcac cccggccagg acgaccaggg
     2701 gcaggaccga gggaagggag cgaacgctgg cgcgggcctt cgtcgccgcc acgattccgc
     2761 cgatcaggag taccgacgcc gtgcggctcg cgaccaaggc ccagatgccg ccatcggcgg
     2821 ccgcccgatc gatgatcagg taccacgagc tgaagccgat cgcggcgccc agcgcgagca
     2881 ggagcgcgga tcggctcgcc gcatcacgcg agatatcagc ggcggccagc ccggcggcca
     2941 cgatgagggc cacgccgacc agcgcgatcg gagcgatctg ggtcccgagc agcaaggtgg
     3001 cgagcagcgg gatcacggcg gcccccgccc cggacaggga ggacacgatg cccatcgagc
     3061 cccgggccag cccttcgtac aggaacgcca ggccaagccc accgccgagt ccggcgaccg
     3121 ctcccagcag gagcgtgtcc gcggcgggga tgggcggcgc gaggagcagg acgccgacca
     3181 ccagcgcgac cagcccgatc acctgggagc cggcggtgac cgcgaacggg gcgacgcgcc
     3241 gcgaagcgag acctccggtg aagtcggccg tgccccacgc cgcggcggtc gcaagccccc
     3301 acagcacgcc tggctggatc acccatctcc tccgagcagg ccccggactg taccagccac
     3361 ttcgaccttg acactcgcgc cgccgcggcc gcacagtgcg ccaatctcgc gcgtcggaag
     3421 cccccgtcgc ccgaccgatc ccgaggtgtc cgtggcgatc gaacctgccg ctccagaggt
     3481 cgcatcggct ccaggcgcgg ccgcccctgc ggtcgttctc gagaatctcg agcggaccta
     3541 tggcgacgtg gtcgcagtgg ccggcatcaa cctcagcatc gccgatggcg agttcttctc
     3601 gatgctcggc ccgtccggct cgggcaagac caccacgctc cggatgatcg ccgggttcga
     3661 gattcccacc gccggacgcg tgctcctgca cggccaggac gtgagcacaa tcccgccgtt
     3721 cgaccgcgac gtgaacacgg tcttccagga ctacgccctg ttcccgcaca tgaccgtggg
     3781 tgacaacgtg gcctacggcc tcgagatccg gaaggtggcg cgcgccgagc gtgagcgacg
     3841 ggtgggcgag gcgatggcca tggtccggct ggagggctac gagaagcgcc gcccggggca
     3901 gctgtccggc ggccagcgcc agcgggttgc cctggcccgg gcccttgtca accggccccg
     3961 ggtcctcctc ctcgacgagc cgctgggcgc gcttgacctc aagcttcgcg agcagatgca
     4021 gatcgagctg aaggagatcc agaaggaggt ggggatcacc ttcatctacg tcacccacga
     4081 ccaggaagag gcgctcacga tgagcgaccg gcttgccgtg ttcaatcggg gcctgatcga
     4141 gcaggtcggg acgccggcgg acgtctacga gcacccgaag accccgttcg tggctggctt
     4201 cgttgggacc tccaacctgc tcaaggacga agcggcccgt gtcgtcatcg gtcaggcggg
     4261 tacgttcact gttcgtccgg agaagatccg gctggcggcg ccagaggacg ctgtcgcatc
     4321 cgacgagcat tcggcgctcg ggaaggtgag cgaagtcgtg tacctcggct cggatacgcg
     4381 gtatatcgtt gcgctcgacg ccggtggcga gctcgtcgtc acccagcaga acctgcagac
     4441 ctcatccatg gaggccctgg ctgcccaggg acggccggtc cggctgatct ggcgacggca
     4501 gcacgtgttg agcctggccg acggccagga atcggcgggg gagatagagg gagttgaacg
     4561 cccatgaggc tcttgcgtat tccggtcatg ctcaccgtgt tcggcttgct ggccgccgct
     4621 tgcagcggcg gagcccccag cggagcgctc accgagctgc ccgacgccga aggcgcgctg
     4681 aacctggtca tctgggccgg gtacgccgag gacggaacca acgatccgaa ctacgactgg
     4741 gtgaatccct tcgtagagga gaccggctgc gaggtcaaca ccaccgacat gacggactcc
     4801 gccaacggcg tcgagctgct gcagagcggc cagtacgacg gcgggtcgtt ctccggtgac
     4861 gccaccctgc ggctgattgc cggcgaggac gtggccccga tcaacaccga actcatcccg
     4921 tactatgccg acgtcttcga gggtctcaag ggtcagcccc acaacaccgt cgacggggtt
     4981 ccgtacggca cgccgcacgg acgcggcgcc aacctgctcc tgtacaacac cgacaccttc
     5041 accgaggcgc ccaccagctg ggatccgatc tgggacgccg actcgccggc tgccggctcg
     5101 gtcagcgtct acaactcggc gatcttcatc gccgatgccg cgcttcgact gaagaccacg
     5161 aatgacaacg agttcgggat cgatgacccg taccagctca ccgaggagca gttcaacgcc
     5221 gccgtcgagc tgctgacgga gcagcacgcg atcgtgggtg agtactggaa ctacgccacc
     5281 gaccagatca cctcgttcgg cgccggcgac atgctggcgg gtacctcgtg gcagtaccag
     5341 gccaacaacc tgcccgacgc accggtgggg atcgtcaagc ccgcggaggg cacgaccggc
     5401 tggtccgaca cctggatgat cgccagcgag gcggagcacc cgggctgcat gtaccgatgg
     5461 atggactgga tgctgtcacc tgaggccaac gcccaggcga cggtctggtt cggggaagca
     5521 ccggtcacgc aggctgcttg cgatcgggcc gaagagctct cgccgaacca ctgcgaggac
     5581 taccacgcga ccgacgaggc ctacttcgag gacgtggcct tctggagcac gcctcgcgag
     5641 gactgcaacg acgaggacga cgcgacgacc tgcaagacga tcgaggactg gatccaggcc
     5701 tggaccgaga tcaccggcag ctgattcgcc gtcggtgacg ttctgaccac acggctcccc
     5761 gggggtccgc ctccggggag ccgctttcca tcccgccaca gaggcaccac tccgaagcca
     5821 tgacctcgtc cacgatgtcg ccatccccgt cacggggccg gcggttcgcg acctggctca
     5881 gcggtcgacc gcggctgcag ctggcgctcc tgctcgccct gccggccggc ttcttcgtca
     5941 tcttctacct gggttcgctg ctgctcctgt tcctgaacgc gttctgggag acggacccgt
     6001 tcagcggcga cgtcatcccg gtcttctcgc tcgacgcgtt cgtgacgatc ttcaccgtcg
     6061 atgtgtatcg gatcatcgcg gcccgcacgc tggggatggc aatcgtcgtc accctgacct
     6121 gcgtcgcgct cggcttcccg gtcgcctact acatggcccg catcgcctcc ccgcgcaccc
     6181 ggcggttgat ggtcatcgcc gtcctgatgc cgctgtgggc gagctacctg gtcaagatct
     6241 acacctggcg cacgatgctg ggtggtaacg gcttcgtgga ctggctcctc gccccgtttg
     6301 ggctcgaggg gccgggcctc aactcgctgg tgggcagctg gctggtcctg acctacctct
     6361 ggctgccgta catgatcctg cccatccagg ccgggctgga gcggatccct ggatccctgc
     6421 tcgaggcatc cgccgatctc ggtgcccgct ccggccgaac cttccgacga gtagtgatcc
     6481 cgctcgtgct gccttctatc gccgccggat cgatcttcac gttctcgctg accctgggtg
     6541 actacatcac cgcgacaatg gtctcgtcga ccaagttcat cggcaacgtg atctacgaca
     6601 actcggcgct gggtagcctg cccctggcgg ccgcctactc gtttgttccg atcgtcatca
     6661 tgatcgtgta cctggtcgtc gccaggcgcc ttggcgcctt cgagtcgctg
//
LOCUS       DASYGU010000219         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_549505, whole genome shotgun sequence.
ACCESSION   DASYGU010000219 DASYGU010000000
VERSION     DASYGU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549505"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..176
                     /locus_tag="VGO32_06125"
     CDS             6..176
                     /locus_tag="VGO32_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV7200361.1"
                     /translation="MEAYEREITDRDRTDSTRAVAPLRRAPGALVLDTGELDVHACVA
                     AIVAALPPRPAP"
     gene            173..850
                     /locus_tag="VGO32_06130"
     CDS             173..850
                     /locus_tag="VGO32_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7200362.1"
                     /translation="MTRGDARHLPRTWAYRPGGLVIGLIINLFGGTRTEGLEFLPRTG
                     AAIIVGNHYTMADPIVAGWATCWQIGRVVHMIAKIQIKGWPVLGWLGSQCGVIYVRRG
                     TSDLEAQRALNAVLAAGRPMIIFPEGTRSTTGGLITARNGAALLAFRSGAPIVPIGIT
                     GTEGMLSWRAIFGRRPRATVRIGQPFTLGHRPDGPIDRAELTAATTRIMREVAALLPP
                     AQRGVYG"
     gene            882..1778
                     /gene="ispH"
                     /locus_tag="VGO32_06135"
     CDS             882..1778
                     /gene="ispH"
                     /locus_tag="VGO32_06135"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEV7200363.1"
                     /translation="MTASGASSAPSRPEVRIAERAGFCYGVRLAIDKARQASEAGEEV
                     TTLGQLVHNPGIKADLEARGIQTAELADQVEGGTLVVRAHGVPRDELETLRAKGDIQL
                     VDATCTWVLQSQKAARELDEAGYTVCIIGHENHPEVRGVRSYAGEKHVVVDDMDRSTW
                     ERVPRTKKLGVLSQSTILPSKLEEFAAFCLRRCHDLRVVNTVCPVTLTRQDDSVKLAA
                     EVDAMVVVGGKNSSNTRELAVKCAQVLPDTIHVADADELETEHAGWLTGKARIGITGG
                     TSTPESDLEEVRDRIYALADAA"
     gene            complement(1792..>2411)
                     /locus_tag="VGO32_06140"
     CDS             complement(1792..>2411)
                     /locus_tag="VGO32_06140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200364.1"
                     /translation="DVRRDMRSELGMGETGLLIVQPTRVIPRKGIELTIELVGRLGDP
                     EAVVLITSPAGDEGLDYLIALERQAERAKVNLRYAADRFAPDLEGRPINPAHSLTEAY
                     LAADLIAYPSLYEGFGNAIIEAVFFGKPVFVNRYPVYDADIRPLGFRFVEIAGTVTDE
                     AVEGVRSLLADPDRIAADAEHNFLIGRASLGYDRLEQALRELLPT"
BASE COUNT          380 a          850 c          829 g          352 t
ORIGIN      
        1 ggcggctgga ggcctatgag cgcgagatca ccgatcgcga ccgcacggat tcgacccgcg
       61 ccgtggcgcc gcttcggcgc gcccctggag cactcgtcct ggacaccggt gagctggatg
      121 tccatgcctg cgtggcggcg atcgtggccg cgctcccacc ccggccggca ccgtgacccg
      181 cggcgacgcc cgccacctgc cgcggacctg ggcctacagg ccgggtggcc tggtcatcgg
      241 tctcatcatc aacttgttcg gggggactcg aaccgagggg ctcgagttcc tgccgcgcac
      301 cggcgccgcg atcatcgtcg gcaaccacta caccatggcc gatccgatcg tggccggctg
      361 ggccacctgc tggcagatcg gccgggtcgt gcacatgatc gccaagatcc agatcaaggg
      421 ctggcccgtg ctcggctggt tggggagcca gtgcggggtc atctacgtcc gtcgaggcac
      481 gagtgacctc gaggcccagc gggccctgaa cgcggtgctg gccgccggtc gcccgatgat
      541 catcttcccc gagggcaccc ggagcacgac tggcggcctc atcacggccc gcaatggtgc
      601 cgcgctgctc gcgttccgca gtggcgcccc gatcgtgccg atcgggatca ccggcaccga
      661 aggcatgctc agctggcgcg cgatcttcgg ccgccgtccg cgtgcgacgg tgaggatcgg
      721 ccagccgttc accctgggcc accggccgga cggccccatc gaccgggccg aactgaccgc
      781 ggcgacgacc cggatcatgc gcgaagtggc ggccctgctt ccgccggccc aacggggcgt
      841 ctacggctga ttcgccgatc ggctcctaga atgaggtcgt catgaccgca tcaggcgcct
      901 cttccgcgcc cagccgaccc gaggtccgca tcgccgagcg ggccggcttc tgctacggcg
      961 tgcggctggc gatcgacaag gcgcgccagg cgtccgaggc cggcgaagag gtcacgaccc
     1021 ttggtcagct cgtccacaac cccggcatca aggccgatct cgaagcccgc ggcatccaga
     1081 ccgccgagct cgccgaccag gtggagggcg gaaccctggt cgtccgtgcg cacggcgttc
     1141 cgcgcgatga gctggagacg ctcagggcca agggcgacat ccagctcgtc gacgccacgt
     1201 gtacctgggt gctccagagc cagaaggccg cccgcgagct ggacgaggcg gggtacaccg
     1261 tctgcatcat cggccacgag aaccaccccg aggtccgcgg cgttcgctcg tacgccggcg
     1321 agaagcatgt cgtggtcgat gacatggacc gatccacctg ggagcgcgtg ccgcgcacca
     1381 agaagctcgg cgtcctgagc cagtccacca tcctgccctc caagctcgag gagttcgccg
     1441 cgttctgcct gaggcgctgc cacgacctgc gggtggtgaa cacggtctgc ccggtcaccc
     1501 tcacccgcca ggacgactcg gtgaagctgg ccgcggaggt ggacgccatg gtcgtggtcg
     1561 ggggcaagaa cagcagcaac acccgcgagc tggcggtgaa gtgtgcccag gtcctgcccg
     1621 acacgatcca tgtcgccgat gcggacgagc tcgagaccga gcacgccggc tggctgaccg
     1681 gcaaggcccg gatcgggatc acgggcggga cgtccacccc cgaatcagac ctcgaagagg
     1741 tccgcgaccg gatctacgct ctggctgacg ctgcctgacg cgtccgcccg gtcaggtcgg
     1801 aagcagctcg cggagggcct gttccaggcg gtcgtagccg agactggccc taccgatcag
     1861 gaagttgtgc tccgcatccg ccgcaatgcg gtccggatcg gccaggagtg atcgaacccc
     1921 ctccaccgcc tcgtcggtaa cggtgccggc gatctcgacg aaccggaagc cgagcggacg
     1981 aatatcggcg tcatagaccg ggtagcggtt cacgaacacc ggcttgccga agaacaccgc
     2041 ctcgatgatc gcgttgccaa agccctcgta caggctcggg taggcgatca ggtcggcggc
     2101 gaggtacgcc tcggtcaggg agtgggccgg gttgatcggg cggccctcga ggtcgggcgc
     2161 gaaccggtcc gccgcgtatc gaaggttgac cttcgcccgc tcggcctggc gctcgagggc
     2221 gatgaggtag tccagcccct cgtccccggc cgggctggtg atgagaacga ctgcttccgg
     2281 atcgccgagc cgtccgacca gctcgatggt cagctcgatg cccttgcgcg ggatgacccg
     2341 ggtgggctgc acgatcagca ggccggtctc gcccatcccc agctcggacc gcatgtcgcg
     2401 ccgcacgtcg t
//
LOCUS       DASYGU010000220         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_86034, whole genome shotgun sequence.
ACCESSION   DASYGU010000220 DASYGU010000000
VERSION     DASYGU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86034"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VGO32_06145"
     CDS             complement(<1..106)
                     /locus_tag="VGO32_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200365.1"
                     /translation="MTGIAILGSTGSIGTQALEVIRAAPDRYELKALAA"
     gene            complement(103..1008)
                     /locus_tag="VGO32_06150"
     CDS             complement(103..1008)
                     /locus_tag="VGO32_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV7200366.1"
                     /translation="MLRTRVLTALVLGPVVLAIAWFREPWLSVGVLLIAAVALWEAGD
                     LLTAAGWPVPRFGMVIAGLVLVIAVLAPFHVDTMPVVGVLDERLLGTGLVLIAMVLAV
                     VGLAIIALRNAEPQAGLSAWMGSVFAVAYLGTLVPLIAVVGHLAPDGGTPDSPLGQFG
                     WASGTGWLLLMFGLVWSCDSGAYFIGRAIGRRKLHPLVSPGKTVEGYVGGAVTAGLVT
                     ALLGWLLLDLAPLIGLVLGLVTAAIAQFGDLAKSMLKRVADRKDSGTLFPGHGGMLDR
                     VDSLLFAAPVLLAGALLFAGMDLAR"
     gene            complement(1011..>1740)
                     /gene="uppS"
                     /locus_tag="VGO32_06155"
     CDS             complement(1011..>1740)
                     /gene="uppS"
                     /locus_tag="VGO32_06155"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV7200367.1"
                     /translation="AQRSGDHGGLNDLSTTEGPRHVAIIMDGNRRWAASRGLPGIAGH
                     VQGVQSIVPVVRHAPDCGIEFLSLYVFSRENWSRTEAEVSGLFSLVDRAVRQYTDELI
                     TEGVSVHVIGRLHEAPTDVQRSIRDAEERTRDGARMHLNIAFNYSGRSEIIDAVRALV
                     ASGHPATDIDEDTLAEHLYTAGQPDPDLIIRTGGEHRTSNFLLWQGAYAELVVSEVLW
                     PDFKPEDLDAAVEEFRGRHRRFGS"
BASE COUNT          303 a          598 c          601 g          238 t
ORIGIN      
        1 cggcggccag cgccttcagc tcgtatcggt caggggcagc gcggatgacc tcgagcgcct
       61 gggttccgat ggacccggtg ctccccagga tcgcaatccc ggtcatcgcg cgagatccat
      121 cccggcgaag agcagcgcgc cggccaggag gacgggcgcc gcgaagagca gcgagtcgac
      181 ccgatcgagc attccgccgt gtcccgggaa cagggtgccc gagtccttgc ggtctgccac
      241 ccgtttgagc atggatttgg ccaggtcacc gaactgggcg atggccgcgg tgacgaggcc
      301 cagcacgaga ccgatgagcg gtgcgagatc cagcaggagc cagccgagga gggccgtgac
      361 cagcccagcc gtgaccgcgc caccgacgta gccctcgacc gtcttgcccg ggctgaccaa
      421 cgggtggagc ttgcggcgac cgatcgcgcg accgatgaaa tacgccccgc tgtcacagct
      481 ccagaccagg ccgaacatga gcagcagcca gccggtgccg ctggcccagc caaactggcc
      541 gagcggcgag tcgggtgtcc caccatcagg cgcgaggtga ccgacaacgg cgatcagcgg
      601 gaccagcgtc ccgaggtatg cgaccgcgaa cacggacccc atccaggcgg ataggccggc
      661 ctgcggttcc gcgttccgca gggcgatgat cgccaggccc acgacggcga gcaccatggc
      721 gatcaggacc agacccgttc cgaggagtcg ttcgtcaagg acaccgacca cgggcatcgt
      781 gtccacgtgg aagggcgcca gcaccgcgat caccaggacg aggcccgcga tgaccatccc
      841 gaaacgtggg accggccatc ctgccgcggt caggagatcc cccgcttccc acagcgccac
      901 cgccgcgatg agcagcacgc cgacgctcag ccacggctcg cggaaccagg cgatggccag
      961 cacgaccggg ccgaggacga gggcggtcag gacacgggtg cgcagcatgg tcagctcccg
     1021 aaccgacggt ggcggccgcg gaactcctcg accgccgcgt cgaggtcctc cggtttgaag
     1081 tccggccaca ggacctcgga caccaccagc tcggcgtacg cgccctgcca cagcaggaag
     1141 ttgctcgtcc ggtgctcgcc gccggtgcgg atgatgaggt cagggtcggg ctggccggcc
     1201 gtgtacagat gttcggcgag cgtgtcctcg tcgatatcgg tcgccggatg acccgaggcc
     1261 acgagcgctc gcacggcgtc gatgatctcc gagcggcccg agtagttgaa cgcgatgttc
     1321 aggtgcatcc gggcgccatc acgggtccgc tcctccgcat cgcggatcga ccgctggaca
     1381 tccgtcggtg cctcgtgcag ccgaccgatg acgtggaccg atacgccttc ggtgatgagc
     1441 tcatcggtgt actggcgaac ggcccggtcg acgagcgaga agaggcccga cacctcggcc
     1501 tcggtccgcg accagttctc gcggctgaag acgtagaggc tcaggaactc gatcccgcag
     1561 tcgggcgcgt gtcggacgac ggggacgatg gattgcacgc cctggacgtg gcccgcgatg
     1621 cccgggagac cgcggctcgc cgcccagcgg cggttgccgt ccatgatgat cgcgacgtgg
     1681 cgcgggccct cggttgtgga caggtcgttc agacctccat gatctccgct tctttgcgct
//
LOCUS       DASYGU010000221         6420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1372515, whole genome shotgun sequence.
ACCESSION   DASYGU010000221 DASYGU010000000
VERSION     DASYGU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372515"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VGO32_06160"
     CDS             <1..453
                     /locus_tag="VGO32_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7200368.1"
                     /translation="AMWLAFGTLLSVVFKRAATAALVGFGVWLLVALFGRFLLGLIQA
                     IIFPVDASLPAADQLAGYQWHALLNRLLPPTLYNEASSVLLNPSVTQTSVPATLGQLD
                     QAQQQIPTLLSLDQSLLLVWPHVVVMVAITVICFAYAYIRFMREEVRA"
     gene            481..1194
                     /locus_tag="VGO32_06165"
     CDS             481..1194
                     /locus_tag="VGO32_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200369.1"
                     /translation="MAEGSVECANCGRANPDWAQVCRSCGAPMQHAATGDQAASTLPG
                     PLAGVDQGSLMAIGGALGTIVAAIIIGAIIGGILPPAPPVAVASPTPGASASIEPTPA
                     PSIDPSASVGPSATPAPVLPGTLTFGLGLNSETQRVTNPTDTFTPGTTFAHSIELTAP
                     FGVTQIGEGVWQIAEDGTETQAINPEENQLTVDPDASIDGFTVPTNALLDPLGPGTFV
                     MRVYRGSELLAQGTFILAE"
     gene            complement(1178..2065)
                     /locus_tag="VGO32_06170"
     CDS             complement(1178..2065)
                     /locus_tag="VGO32_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV7200370.1"
                     /translation="MNAVRRTDGKLTVALVSEVFWQPDGVVRLGQRLDEAARRGADIA
                     LLPELPLHPWHPATKEAVDDDAESMGGPRMTVQAEAARTAQIGLVGGIIHRDESGRRT
                     SRGLVFDSTGELVSTFEKLHLPEEDGFRETSHYQPGTAIARPTDAFGMPVGIQICSDT
                     NRPEGSHLLGALGAEAILIPRASEEKTYQRWKVVFRANALTSAAYVLSVNRPDPEAGV
                     LLGGPSVAVDPSGHTIVETTDRMAVITLDRDVITEARVAYPGYLPIWARLYGEAWNEV
                     AASAESTAEGEAGPPIRPG"
     gene            complement(2062..2370)
                     /locus_tag="VGO32_06175"
     CDS             complement(2062..2370)
                     /locus_tag="VGO32_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200371.1"
                     /translation="MNDGRTAPLTYDDLEAIARRFEGKPLETVRGKAFTVGISRNGEI
                     FFIPASSAWGQTEGRKGHQRFVERFNEIGSLQPKDYADVSRNASYLIGLLKAAQEEST
                     "
     gene            complement(2367..3467)
                     /locus_tag="VGO32_06180"
     CDS             complement(2367..3467)
                     /locus_tag="VGO32_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7200372.1"
                     /translation="MTTVPLSRAATIPSRFYLDPGILEAEKDRIFSRTWQLVARSEDL
                     ARIGDFVPATIVDEPIVITHGLDGELRGFYNVCRHRAGQVALARGNRKSLQCRYHGWT
                     YGLDGTLRACPEMEATEDFRKEEFGLVPIRVEPWGPFVFANLDPDAPPLADVLGSIPA
                     EVEAARFDLSHMRLVERREYVIDCNWKVYVDNYLEGYHLPIAHPDLFKEVDYDAYRVE
                     EHRYYSKQYAPIRDLKPGEEVGRDRRFMRRADGEDSALYYWVFPNTMFNIYPDNLSSN
                     VVLPLGHERTLTVFEWFFAEPGTGPGWESMQQGIAFSDQIQQEDIVLCEQVQRGLRSR
                     AYDSGRFSALRENGVYHFQNLVREFLAEEENR"
     gene            complement(3464..5362)
                     /locus_tag="VGO32_06185"
     CDS             complement(3464..5362)
                     /locus_tag="VGO32_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7200373.1"
                     /translation="MSALSSRPLLVFGLVVVAGIGVWAAASVLIKPNPVFVSERACED
                     SAFSCVTINVPRDHFNPGGPTWEVSFAIQRALSGTRDGVIVIATGGPGSSGIAVADDY
                     SAYFDAEIPERYDIVFFDQRGIGRSRPLQCPEAAVAWYSSPIMPTVSGADAEAYAGAT
                     ESFVADCLEETGVDHADLPYFSTEQASEDLEVFLDWLGAEQIDLYGESYGTQYAQVFA
                     AAHPERIQALLLDGSVDLTVDGTDYHVEATHAHADTLAMILDLCWQDSACQTDFGGED
                     PLDSYDALVSALQDGPMTYDFVTDEGVVETREFSWADLETAVADNVTPTYWRMLLQRA
                     LAQAARGELLPLARLDYLALGQNADTLTTIPDPTWSDALYYAVDCTDYAFGSGSAADR
                     ADEYLAAGEDAGVADLRLGSVYYGNLPCAYWPVHGPEERPAYLTDTSYPVFVLAATAD
                     PATPYAGSERIVAQLEDPYFIVQPGGPHVIYLRGNPCPDDVIAAFLLDGTLPERETTC
                     DLPAVEPYVPIPAADVSDYDSTLDALSAVDDEINNNADFWDWDLTKPLTYGCQFGGTI
                     SYRVTDQGYEVTLDDCAFSRGLDLTGDAVINDAKFSFSLTAQTGDGSELTYERDGDGE
                     RTATGNLP"
     gene            5515..6348
                     /locus_tag="VGO32_06190"
     CDS             5515..6348
                     /locus_tag="VGO32_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7200374.1"
                     /translation="MKSAARGAAVFAALAIALAACSGGGGDSLTTIQDEGTIQISTDP
                     NYEPQSFLDDNGELIGFDIDVAREIADRLGVEVEFVTPTFDLVQAGGWNGQWDVSVGS
                     ITVTDARKEVLDFTPPYYFTPAQLAATEASGIASIEDFAGKKICVGAATTYLDWINGD
                     LVLGDGSELAPVPDGVEAVALETDALCAESIDAGRDEFDGWLSSSTTVAAAIDGGVPI
                     VTVGDPVFYEPLAVATDKSGPDNDALQAELDDIINAMHEDGTLTDMSMEWYGIDITTK
                     E"
BASE COUNT          996 a         2183 c         2207 g         1034 t
ORIGIN      
        1 gcgatgtggc tggccttcgg cacgctgctg tcagtcgtat tcaaacgcgc cgcgaccgcc
       61 gccctggtcg ggttcggcgt ctggctcctg gtcgcgctct tcggccgctt cctgctgggc
      121 ctgatccagg ccatcatctt cccggttgat gccagccttc cggcggccga ccagctggcc
      181 ggttaccagt ggcacgccct cctgaaccgg ctcctcccgc cgacgctgta caacgaggcg
      241 tcgtcggtcc tcctcaaccc gtcggtgact cagacctcgg tgccggccac cctgggccag
      301 ctcgatcagg cccagcagca gattccgacc ctcctgtcgc tcgaccagag cctgctcctc
      361 gtctggccgc atgtcgtggt catggtcgcg atcacggtca tctgcttcgc ctacgcctat
      421 atccgcttca tgcgcgagga ggtccgggcc tagccagtcg ccgtgctagg ctccccgccg
      481 atggccgagg gatccgtgga gtgcgccaac tgcggtcggg ccaatcccga ttgggcccag
      541 gtctgccgct cgtgcggagc gccgatgcag catgccgcga ccggcgacca ggcggcatcc
      601 acgcttcccg gtccgctcgc cggcgtcgac cagggttccc tgatggccat cggtggagcc
      661 ttgggcacga tcgtggccgc gatcatcatc ggcgcgatca tcggcggcat cctgccgccc
      721 gccccgccgg tcgcggtggc ctccccgacg ccgggggcat cggccagcat cgagcccaca
      781 cccgcgccgt cgatcgaccc cagcgcgtcc gttggcccgt ccgccacccc cgcgcccgtc
      841 ctgcccggca cgctcacgtt cggactgggg ctgaactccg agacgcagcg ggtcacgaac
      901 ccgactgaca cgttcacgcc gggcaccacg ttcgcccact cgatcgagct caccgccccg
      961 ttcggggtga cccagatcgg ggagggcgtg tggcagatcg ccgaggacgg aaccgagacc
     1021 caggccatca accccgagga gaaccagctc acggtggacc cagatgccag catcgacggc
     1081 ttcacggtgc cgaccaacgc gctgctcgac ccgctcgggc cgggcacgtt cgtgatgcgg
     1141 gtctaccgcg gctccgagtt gctggcgcag ggcacgttca tcctggccga ataggcggtc
     1201 cggcctcgcc ctccgccgtg gattcggccg aggcggcaac ctcgttccat gcctctccgt
     1261 acagccgcgc ccagatcggc aggtagccgg ggtatgcgac ccgggcttcg gtgatgacgt
     1321 cccggtcgag ggtgatcacc gccatccgat cggttgtttc gacgatggtg tgtccactgg
     1381 ggtccacggc caccgacggc ccaccgagga ggacgcccgc ctcgggatcc ggccggttga
     1441 ccgacaggac gtacgcggcc gaggtcaggg cgttcgcccg gaagacgacc ttccagcgct
     1501 ggtaggtctt ctcctcggac gcgcgcggga tgaggatcgc ctccgcgccc agcgccccga
     1561 gcaggtgcga cccctcgggc cggttggtgt ccgagcagat ctggatcccg accggcatcc
     1621 cgaacgcatc ggtcggtcgt gcgatcgccg tcccgggctg gtagtggctc gtctcccgga
     1681 agccgtcctc ctcggggagg tgaagctttt cgaaggtcga caccagctcc ccggtcgagt
     1741 cgaagacgag gccgcggctg gttcggcgtc cgctctcgtc gcgatggatg atcccgccca
     1801 ccaggccgat ctgcgccgtg cgcgccgctt ccgcctggac cgtcatccgg ggaccgccca
     1861 tcgactccgc gtcgtcgtcc acggcctctt tcgtggccgg gtgccaggga tggagcggga
     1921 gctcgggcag gagggcgatg tcggctcccc gccgagcggc ctcgtccagt cgttggccca
     1981 ggcgcacgac accgtcgggc tgccagaaga cctcactgac gagcgcaacg gtcagcttgc
     2041 catcggtccg tcgtacggcg ttcatgtgga ttcctcctgc gcggccttga gcaggccgat
     2101 gaggtacgag gcgttgcgac tgacatcggc gtagtccttg ggctgcaggc tgccgatctc
     2161 gttgaagcgt tcgacaaagc gttggtggcc ctttcggcct tcggtctggc cccaggcact
     2221 cgaggccggg atgaagaaga tctcgccatt gcgcgagatg ccgactgtga acgccttgcc
     2281 ccttacggtc tccagcggct tgccttcgaa gcggcgggcg atagcttcca ggtcgtcgta
     2341 cgtcagcgga gccgtgcggc cgtcgttcat cggttttcct cttcggccag gaactcgcgg
     2401 accaggttct ggaagtggta cacgccgttc tcgcgcaggg cgctgaaccg gccgctgtca
     2461 taggcgcgcg agcggagccc gcgctgcacc tgctcgcaca ggacgatgtc ctcctgctgg
     2521 atctggtccg agaacgcgat cccctgctgc atggattccc agccggggcc tgttcccggc
     2581 tcggcgaaga accactcgaa gacggtgagc gtccgctcgt ggccgagggg caggacgacg
     2641 ttggacgaga ggttgtcggg gtagatgttg aacatcgtgt tcgggaagac ccagtagtac
     2701 agggcgctgt cctcgccgtc ggcgcggcgc atgaaccgac ggtcgcggcc cacctcttcg
     2761 ccgggcttca ggtcgcggat gggcgcgtac tgcttcgagt agtagcggtg ctcctccacc
     2821 cggtacgcgt cgtagtcgac ctccttgaac aggtccgggt gggcgatcgg caggtggtag
     2881 ccctcgaggt agttgtcgac gtagaccttc cagttgcagt cgatcacgta ctcccgccgc
     2941 tccacgagcc gcatgtggga caggtcgaag cgggccgcct cgacctcggc ggggatggag
     3001 cccaggacgt cggcgagggg cggcgcgtcc ggatcgaggt tggcgaacac gaacgggccc
     3061 catggctcga cgcggatggg gacgaggccg aactcctcct tgcggaagtc ctcggtggcc
     3121 tccatctcgg ggcaggcacg gagcgtcccg tccaggccgt aggtccagcc gtgataccga
     3181 cactggaggc tcttccggtt gccccgggcg agggcgacct ggccggcccg atggcggcac
     3241 acgttgtaga agccgcggag ctcgccgtcc agaccgtgag tgatcacgat cggctcgtcg
     3301 acgatggtgg cgggcacgaa atcgccgatc cgggcgaggt cctccgaacg ggccacgagc
     3361 tgccaggtcc ggctgaagat ccgatccttc tccgcctcga ggatgccggg gtccaggtag
     3421 aagcgcgacg ggatcgtcgc cgcccgggac agcggaaccg tggtcatggc agatttcccg
     3481 tggccgtccg ctcgccgtcg ccgtcacgct cgtacgtcag ctccgagccg tcgcccgtct
     3541 gggcggtcag gctgaacgag aacttcgcgt cgttgatgac cgcatcgccg gtcaggtcca
     3601 ggccacgcga gaacgcgcag tcgtcgaggg tgacttcgta gccctggtcc gtgacccgat
     3661 acgagatcgt gccgccgaac tggcagccat aggtcagcgg cttggtgaga tcccaatccc
     3721 agaagtcggc gttgttgttg atctcatcgt ccaccgccga gagggcgtcc aacgtggagt
     3781 cgtagtcact cacgtcggcg gccgggatgg gcacgtacgg ctcgaccgcg ggcaggtcgc
     3841 aggtcgtctc ccgctcgggc agcgtgccgt cgagcaggaa cgcggcgatc acgtcgtccg
     3901 gacacgggtt gccgcggagg tagatgacat gcgggccgcc cggctggacg atgaagtatg
     3961 gatcctcgag ctgggccacg atccgctccg agcccgcgta cggagtcgcc gggtcggcgg
     4021 tcgccgccag cacgaacacc gggtacgacg tgtcggtcag gtacgccggg cgctcctccg
     4081 gaccatggac cggccagtag gcgcacggca ggttcccgta gtagaccgat cccagccgaa
     4141 gatcggcgac accggcatcc tcgccggcgg cgaggtactc gtccgcccgg tcagcggccg
     4201 agccggagcc gaacgcgtag tcggtgcagt ccaccgcgta gtacagggca tcggaccagg
     4261 tgggatccgg gatggtggtc agggtgtccg cgttctggcc gagcgcgagg tagtccaagc
     4321 gagcgagggg caggagctcg ccccgtgcgg cctgggcgag cgcgcgctgg agcagcatcc
     4381 gccagtaggt gggcgtgacg ttgtcggcga ccgcggtctc gaggtccgcc cagctgaact
     4441 cgcgggtctc taccacgccc tcatcggtca cgaagtcgta ggtcatcggg ccgtcctgga
     4501 gcgcgctcac cagcgcatcg tacgagtcga gtggatcctc gccgccgaag tcggtctggc
     4561 aggccgagtc ctgccagcag aggtcgagga tcatggccag cgtgtcggcg tgggcatgcg
     4621 tcgcctccac gtggtagtcg gtcccgtcca cggtgagatc caccgacccg tccaggagca
     4681 gggcctggat ccgctcgggg tgggcggcgg cgaaaacctg ggcgtactgg gtcccgtagc
     4741 tctccccgta caggtcgatc tgctccgccc ccaaccagtc caggaagacc tcgaggtcct
     4801 cggacgcctg ctccgtggag aagtacggca gatcggcgtg gtcgaccccc gtctcctcca
     4861 ggcagtcggc cacgaacgac tcggtcgcgc ccgcgtaggc ctcggcgtcc gcacccgaga
     4921 cggtgggcat gatcgggctc gagtaccagg cgaccgcggc ctcggggcac tggagcgggc
     4981 gcgaccggcc aatgccccgc tggtcgaaga acacgatgtc gtagcgctcg gggatctccg
     5041 cgtcgaagta cgcgctgtag tcgtccgcca ccgcgatacc ggacgagccc ggccctccgg
     5101 tcgcgatcac gatcaccccg tcgcgggtcc cgctcagggc gcgctggatg gcgaacgaga
     5161 cctcccacgt cggcccgccc ggattgaagt ggtcgcgagg gacgttgatc gtcacgcagc
     5221 tgaacgcgct gtcctcgcag gcgcgttcgc tcacgaatac cgggttgggc ttgatgagga
     5281 cgctggccgc ggcccacacg ccgatgccag ccaccacgac caggccaaag acgagcaggg
     5341 ggcggctgga cagggccgac atcggcggca tgctagcagc cgcgctgacg ctcaccggga
     5401 gcgagttcga gccttgccgc gctgccgtgc gaagtgctac aaccaccctc tacgcgagcg
     5461 gcagacggga gacgagcccg actgacggct gccaccgccc actggaggag aggaatgaag
     5521 tctgctgcac gaggcgccgc cgtattcgcg gcgctggcga tcgctctggc cgcgtgttcc
     5581 ggaggcggtg gggactccct gacgaccatc caggacgagg ggaccatcca gatctcgacg
     5641 gacccgaact acgagcccca gtcgttcctc gatgacaacg gcgagctcat cgggttcgac
     5701 atcgacgtcg ccagggagat cgccgatcgc cttggcgtcg aagtcgagtt cgtgacgccc
     5761 acattcgacc tggtccaggc cggcggctgg aacggccagt gggacgtttc ggttggctcg
     5821 atcacggtca ccgacgcgcg gaaggaagtc ctcgacttca ccccgcccta ctacttcacg
     5881 ccggcgcagc ttgccgcaac ggaggcctcg ggcattgcga gcatcgagga cttcgcgggc
     5941 aagaagatct gcgtgggcgc ggcgacgacc tacctcgact ggatcaacgg tgacctggtc
     6001 ctgggcgacg ggtcggagct ggccccggtg cctgacggtg tcgaagccgt ggcgctcgag
     6061 acggacgcgc tgtgcgcgga gtcgatcgac gccggacgcg atgagttcga cggctggctc
     6121 agctcgtcga cgacagtggc cgcggccatc gacggcggcg tgccgatcgt gaccgtgggc
     6181 gacccggtgt tctacgagcc cctcgcggtc gcgacggaca agagcggtcc cgacaacgat
     6241 gcgctccagg ccgagctcga cgacatcatc aacgcgatgc atgaagacgg cacgctcacc
     6301 gacatgagca tggagtggta cgggatcgac atcacgacga aggagtaggt ccgcccctgt
     6361 ccagaaccta ggaacgggga cgcacaagcg tccccgttcc gcacttaagg gaggcatccc
//
LOCUS       DASYGU010000222         6797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1732521, whole genome shotgun sequence.
ACCESSION   DASYGU010000222 DASYGU010000000
VERSION     DASYGU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732521"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="der"
                     /locus_tag="VGO32_06195"
     CDS             <1..950
                     /gene="der"
                     /locus_tag="VGO32_06195"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV7200375.1"
                     /translation="PPPDPNAEPEPRPEPDASDWAGEFPTDAELAELAETDVGPPRVA
                     IVGRPNTGKSTLVNKVLGQERMIVSDVPGTTRDAIDTEVVVDGETLVLIDTAGIRRRG
                     SIAAGTERYSVLRSLKAIDRADVAVVLTDATERFTAQDAHVVGYVLDAGKGLVLVINK
                     WDQIEKDEHTADQWLRKLRRDAPYLEWADIVFASAMTGQRVERILREARRVAEERYRR
                     VATAEVNRVVGDAVAAHPPPTARGRRARVLYATQVAVAPPTFIIFVNDPELFHFSYRR
                     YIENRLREAFGFAGTPIRLRFRARTSERPRGRRSKASAR"
     gene            973..1983
                     /locus_tag="VGO32_06200"
     CDS             973..1983
                     /locus_tag="VGO32_06200"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV7200376.1"
                     /translation="MTERIAVLGAGAWGTTLARMLAASEHPVTLWAHRPEAAAEMVRD
                     GQNRRYLADYPFPAGLLVTGDVSELRAPHRLYILAVPSAHARGILRRVGADLAHETPF
                     LSVVKGIEARTQARMSEVIAEELPGRAIAVLSGPNLADEVASGLPAGTVVASVDPALA
                     ADVAELLGSDQFRVYTNPDVTGVELAGALKNVIALAAGLADGLRMGDSGKAAIVTRGL
                     AEITRLGVAAGADARTFAGLAGIGDLIATCMSQLSRNHRAGELLATNMSWADVATQLG
                     GVAEGESTVTGALALAQRHGVEMPIAEQVDAVIHRGRAPLEALATLMARVQKDELSGR
                     ET"
     gene            complement(1980..4040)
                     /locus_tag="VGO32_06205"
     CDS             complement(1980..4040)
                     /locus_tag="VGO32_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7200377.1"
                     /translation="MTDTAEADASHDAAHELRRLQSTRGRLAGDQYVRVIEPFASSFH
                     REAPGHLIASEEVLAPRGILGRGIESVRRLAIGSRIATDRELHERIGAVKGLAVFASD
                     NISSSAYATEEIMRVVVLAGVGALVLTMPLTIAIVVVLAVVVVSYQQTIRAYPNGGGS
                     YIVASANLGRGAGLTAAAALLTDYVLTVAVSIAAGVAALTSIFPGMYDYRVLVGLGFI
                     GVLWIGNLRGIRDSATVFAIPTYIYLVAIYVLLALGLYQLATGTMAPYSPPPPWLDAE
                     GSQALGILLILRAFASGSVALTGTEAVSNGVPAFKRPEARHAGRVLILMGVFFGTIFV
                     GISYLASQIGIVPDPTEQETVISQITRTIVGAGSPFHYLVQLSTALLLVLAANTAFAD
                     FPRLASILSRDGFMPRQFQFRGDRLAFSVGIMALGVLAGILIVVFEGSVTNLIPLYTV
                     GVFVAFTLSQSGMVRHWWLLRGEQPGWRWRASLNGAGAVATGVVAIVVGVAKFALGAW
                     MVLLLIPLLMGLMAAIHGHYRSTERSLEVLGPPEALPEREPRVVVPVSRFDRAALAAL
                     AYARAISSDVTAVHVATDGDEVADMRARWEAWGGPVKLVILESPYRALMAPLLAYLDA
                     TETMDPSRPTTVVLAEFVPRHIWEYPLHNQTALRLKLRLFRRPNTVVIDVPYHVTVSD
                     HRAA"
     gene            complement(4037..4294)
                     /locus_tag="VGO32_06210"
     CDS             complement(4037..4294)
                     /locus_tag="VGO32_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200378.1"
                     /translation="MASALPAASPVTSDHPAADLELAQVRSAIDLITSGTARRVILVG
                     LRGAERLLPRAQVLAADAGLTARAVRHGDGTGCDIAVEPIR"
     gene            complement(4363..5085)
                     /locus_tag="VGO32_06215"
     CDS             complement(4363..5085)
                     /locus_tag="VGO32_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7200379.1"
                     /translation="MTEEPRTGPTILLVEDDAGTRAEVARNLRAHRYRIVEAATAAAA
                     TRAWDARRPDLVLLDLGLPDRDGIVVVERIRRDSSVPVLVLSARGEEATKVAALEAGA
                     DDYVTKPFSMVELQARIRALLRRSAGPEGDPAGTLRNGTLEVDPAAHEVRVAGQPVTL
                     VPREFQILAELAAHPGRLVTRGRLLRAVWGTAYSGEDHYIHVYVSQIRKKLAAADPGG
                     ELADLLVAEPGVGYRVRIPPPT"
     gene            complement(5082..6650)
                     /locus_tag="VGO32_06220"
     CDS             complement(5082..6650)
                     /locus_tag="VGO32_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7200380.1"
                     /translation="MTARGSRLVGPMKAAIAVAALLAATTALIAVLERWVGVPDASST
                     YLLAVLASAVYFGVFAAIATAVGAFLLYNFLFVDPNFTLAVSDPGELLNLILLLVLGI
                     AVGQLAAAQRSRAQVAIEREHEARALFRISRTLATRTDTAAVLGELGATVHAEAAMTR
                     VWIGIARPSGGERVAADTGDGTQGVDASVEHVILRRMPGDTPARWVQVNPPTAGRTID
                     PDMRVYRVAIEAADESLGSIWATRPRSVGEPGRSATRLLAAAADQIGQAIEQDRLAAE
                     ARIAEVARRSDAVKTALLESVSHDLRTPLATIRAAAGTILEGPEAVPEADRRASAEAI
                     DREAEHLNRMVTNLLDMGRIEGGALRADREALAVEESARETVDRYRPRLADLELTYDW
                     AEGLPPVYADPVFLGQVMANLLDNAASFVPSGGQVRIGARMVDGMVQIRVEDSGPGVA
                     PDALPHLFEKFYRSGSPEAGSRTGTGTGLAVVRGLTEAMGGHAEAHPSELGGLAVDVS
                     LPVAPAPPGGGSDS"
BASE COUNT         1046 a         2347 c         2455 g          949 t
ORIGIN      
        1 tgccaccgcc cgatccgaac gcggaacccg agcctcggcc ggaaccggat gcatcggact
       61 gggccggcga attcccgacc gatgccgagc tggcggagct ggccgagacc gatgtcgggc
      121 cgccccgggt cgccatcgtg ggccggccga acaccggcaa gtccacgctc gtgaacaagg
      181 tcctcggaca ggagcgaatg atcgtcagcg atgtgccggg gaccacccgg gacgcgatcg
      241 acaccgaggt tgtcgtcgac ggcgagacgc tggtgctcat cgacaccgcc ggcattcggc
      301 gtcgcggatc gatcgcggcc ggcaccgagc gatacagcgt cctgcgcagc ctgaaggcca
      361 ttgaccgcgc cgacgtcgcg gtcgtgctga ccgatgcgac cgagcgcttc accgcccagg
      421 acgcccacgt ggtcgggtac gtgctcgacg ccggcaaggg gctggtcctg gtcatcaaca
      481 agtgggacca gatcgagaag gacgagcaca ccgccgatca gtggcttcgc aagctgcgac
      541 gtgacgcgcc ctatctcgag tgggcggaca tcgtgttcgc ctcggccatg accggccagc
      601 gcgtggagcg gatcctccgc gaggcgcgcc gagtcgccga agagcgctat cgccgtgtgg
      661 ccaccgctga ggtcaaccgg gtggtcggtg acgcggtggc ggctcatcca ccgcctaccg
      721 cccgcggtcg ccgtgcgcgg gtcctgtacg cgacccaggt cgcggtggcg ccgcccacgt
      781 tcatcatctt cgtgaacgac ccggaactgt tccacttctc ctaccgccgc tacatcgaga
      841 accgcctgcg ggaagcgttc ggcttcgcgg ggacaccgat ccgccttcgc ttccgtgctc
      901 gcacctcgga gcggccgcgc ggacggcgct cgaaggcttc ggcccgctag cggccgggat
      961 atcctcggcc gcgtgacgga gcggatcgcg gtcctcggcg ccggggcgtg ggggacgacc
     1021 ctggcccgca tgctggccgc atcggagcat ccggtcacgc tctgggcgca ccgtccggag
     1081 gccgcggcgg agatggtccg ggacgggcag aaccggcggt acctggccga ctacccgttc
     1141 ccggccggct tgctggtcac cggtgacgtg tccgagctcc gagcgccgca ccggctgtac
     1201 atcctggccg tgccttcggc ccacgcccgg gggatcctgc gtcgagtcgg cgccgatctc
     1261 gcccacgaga ccccgttcct gtcggtcgtg aagggcatcg aggctcgcac ccaggctcgg
     1321 atgagcgagg tcatcgccga ggagttgccc gggcgcgcaa tcgccgtgct gtccggcccg
     1381 aacctggccg acgaggtggc gtccgggctg ccggcgggca cggtggtggc ctccgtcgac
     1441 ccggcgctgg cggccgatgt tgccgagctg ctcggatcgg atcagttccg ggtgtacacc
     1501 aaccccgacg tgaccggcgt cgagctggcc ggggcgctga agaacgtcat cgcgctggcc
     1561 gccggattgg ccgatgggct ccggatgggg gacagcggca aggcggcgat cgtgacccgc
     1621 ggcctggccg agatcacacg cctgggcgtg gccgccgggg cggatgctcg cacgtttgcc
     1681 gggctggccg gcatcggcga cctgatcgcg acctgcatga gccagctgtc gcgcaaccat
     1741 cgggctggcg agctgctggc cacgaacatg agctgggccg atgtcgcgac ccagttgggc
     1801 ggtgtggcgg aaggcgaatc aacggtcact ggcgcgctcg cgctcgccca gcgccacggg
     1861 gtggagatgc ccatcgccga gcaggtcgac gcggtgatcc accgcgggcg cgcgccactg
     1921 gaggcgctgg ccacgctcat ggcccgggtc cagaaggacg agctcagcgg ccgagaaacc
     1981 taggcggcgc ggtggtccga cacggtcacg tgatatggga cgtcaatgac caccgtgttc
     2041 ggccgccgga agaggcgaag cttcaggcgg agggcggtct ggttgtggag cgggtattcc
     2101 cagatatggc gggggacgaa ctcggccagc acgacagtag tcggccggga cgggtccatc
     2161 gtctcggtgg cgtcgaggta ggcgagcagc ggcgccataa gggcgcggta cggcgactcg
     2221 aggatgacca gcttcaccgg tccgccccag gcctcccacc gggcgcgcat gtccgccact
     2281 tcatcgccat cggtcgcgac gtggacggcg gtgacgtcgc tcgagatcgc gcgggcatag
     2341 gccagggcgg ccagggccgc ccgatcgaat cggctgacgg ggaccacgac ccgcggctct
     2401 cgctcgggca gcgcctcggg cggcccgagg acctcgaggc tgcgctcggt gcttcggtag
     2461 tggccgtgga tggcggccat gaggcccatg agcagcggga tcaggagcag gaccatccac
     2521 gccccgagcg cgaacttggc cacgcccacc acgatggcca ccacccccgt ggccaccgcg
     2581 ccggcgccgt tcaaggacgc ccgccatcgc cagccaggct gctcgccgcg cagcaaccac
     2641 cagtgccgga ccatccccga ctgactgagc gtgaaggcga cgaagacccc gaccgtgtac
     2701 agcgggatga ggttcgtgac gctcccctcg aacaccacga tcaggatgcc cgccagcacg
     2761 ccgagggcca tgattccgac gctgaaggcc agccgatcgc cgcggaactg gaactggcgg
     2821 ggcatgaagc cgtcgcggct gagaatgctg gccaaccgcg ggaagtcggc gaacgcggtg
     2881 ttcgcggcga ggaccaggag gagggcggtc gacagctgga cgagatagtg gaacggcgag
     2941 ccggcgccca cgatcgtccg ggtgatctgg ctgatcaccg tctcctgctc ggtcgggtcc
     3001 ggaacgatcc cgatctggct ggccaggtag ctgatcccca cgaagatcgt gccgaagaag
     3061 acacccatca gaatcaagac ccggccggca tgccgggcct cgggccgctt gaaggcagga
     3121 accccgttcg agaccgcctc ggtcccggtc agggcgaccg aacctgaggc aaacgcacgc
     3181 aggatgagga ggatgcccag tgcctgggaa ccttcggcat cgagccacgg cggcggtggg
     3241 ctgtacggcg ccatggtccc ggtcgccagc tggtacaggc cgagggccag gagcacgtag
     3301 atcgcgacca ggtagatgta ggtcgggatc gcgaacacgg tcgcgctgtc ccggatgccc
     3361 cgcaggttgc cgatccacag cacgccgatg aacccgagac cgacaaggac ccgatagtcg
     3421 tacatgcccg ggaagatgga ggtcagcgcc gccacgccgg cggcgatcga gacggcaacg
     3481 gtcaggacgt agtcggtcag cagtgccgcg gccgcggtga ggccggcgcc acgacccagg
     3541 ttggcactgg ccacgatgta cgatccgccg ccgttgggat aggcgcggat ggtctgctgg
     3601 tagctcacca ccaccacggc caggaccacg acgatcgcga tcgtgagggg catggtcagg
     3661 accagcgcgc ccacgccggc cagcacgacg acgcgcatga tctcctcggt ggcgtaggcc
     3721 gaggacgaga tgttgtccga ggcgaagacc gccagaccct tcaccgcgcc gatccgctcg
     3781 tggagctcgc gatcggtcgc aatccggctg ccgatggcca gccggcgcac ggactcgatc
     3841 ccgcggccga ggatcccgcg cggggcgagg acctcttccg aggcgatcag gtgccccggc
     3901 gcctcccgat gaaacgagct ggcgaacggc tcgatcacgc ggacgtactg gtcgccggcc
     3961 aggcggcccc gggtcgattg gaggcggcgc agctcgtgcg ccgcgtcgtg tgaggcgtct
     4021 gcctccgcgg tatcggtcat cgaatgggct ccaccgcgat gtcacagccg gtgccgtcgc
     4081 cgtgtcggac ggcacgcgcg gtgaggcccg catcggcggc caggacttgt gcgcgcggca
     4141 ggagacgctc agccccgcgg agcccgacga ggatcacgcg acgcgcggtg cccgaggtga
     4201 tcaggtcgat cgcggatcgg acctgggcca gctcgaggtc ggccgccggg tgatcggacg
     4261 tgacggggga cgcggctggg agggcggatg ccatgaactc acgatcgcag acgctccaag
     4321 agagaacgac ggccgcgcca ggcccgttcg ctcaaggagc gctcaagtcg gtggcggaat
     4381 ccgcacccga tagccgactc ccggctcggc gaccagcagg tcggccagct caccgccggg
     4441 atccgccgcg gccagctttt tccgaatctg gctcacgtac acgtggatgt agtgatcctc
     4501 gccgctgtac gcggtccccc agaccgcgcg caggagccgg ccccgggtga ccagtcgccc
     4561 gggatgggcc gcgagctccg ccaggatctg gaactcgcgc ggcacgaggg tgaccggttg
     4621 ccctgcgacg cggacctcgt gcgccgccgg atccacctcg agcgtcccgt tgcgcagagt
     4681 gccggcagga tcaccctcgg gtccggcgct ccgtcggagc agggcccgga tccgcgcctg
     4741 gagctcgacc atgctgaatg gcttggtcac gtagtcgtcg gcgccggcct cgagggcggc
     4801 caccttggtc gcctcctcgc cgcgggcgga gaggacgagg accggcacgc tgctgtcccg
     4861 ccggatgcgc tcgacaacga cgattccgtc gcgatcgggc aatcccaggt ccaggagcac
     4921 gaggtcgggc ctccgtgcgt cccaggcacg cgtcgccgcg gcggcggtcg cggcctccac
     4981 gatccggtat cgatgagcgc gcaggttgcg ggcgacctcg gcgcgggtcc ctgcgtcgtc
     5041 ctcgacgagc aggatcgtcg gcccggtccg tggctcctcg gtcatgagtc cgatccaccg
     5101 cccggtggcg ccggggcgac ggggagcgag acgtcgaccg cgaggccgcc cagctcgctg
     5161 gggtgagcct cggcgtggcc gcccatcgcc tcggtcaggc cgcggacgac cgcgaggccg
     5221 gttcccgtgc ccgtgcggct gcctgcctcg ggcgatccgg atcggtagaa cttctcgaag
     5281 aggtggggga gcgcatccgg cgcgaccccg ggtccgctgt cctcgacccg gatctggacc
     5341 atgccgtcca ccatccgagc tcctatccgg acctggccac ccgatggcac gaacgatgcc
     5401 gcattgtcca ggaggttggc catgacctgg cccaggaaca cggggtcggc gtacactggc
     5461 ggaaggcctt ccgcccagtc ataggtcagc tcgagatcgg ccagtcgcgg ccgatatcga
     5521 tccaccgtct cgcgagccga ttcctcgacc gccagcgctt cgcgatcggc ccgcagggca
     5581 ccgccttcga tccggcccat gtcgaggagg ttggtcacca ttcggttgag gtgctccgcc
     5641 tcccgatcga tggcttccgc gctcgctcgc cggtccgcct cgggcacggc ctccgggcct
     5701 tcgagaatgg tgcccgccgc ggcccggatg gtggccagcg gcgtgcggag gtcatgggat
     5761 accgattcga ggagggccgt cttcaccgcg tcggagcgtc gtgccacctc ggcgatgcgc
     5821 gcctcggccg ccagccggtc ctgttcgatc gcctgaccga tctggtcggc cgccgcggcc
     5881 agcagtcggg tcgcactccg gcccggttcc ccaacagatc gaggccgggt cgcccagatc
     5941 gagcccaggc tctcgtccgc ggcctcgatc gcgacccggt agacccgcat gtcggggtcg
     6001 atcgtccggc ccgcggtcgg cggattgacc tggacccagc gagccggggt gtccccgggc
     6061 atccggcgca ggatcacgtg ctccaccgag gcgtccactc cctgggtccc gtccccggtg
     6121 tcggcggcca cccgttcgcc gccactcggt cgggcgatcc cgatccagac ccgggtcatg
     6181 gccgcttcgg cgtgcacggt ggccccgagc tcgccgagca ccgcggcggt gtcggttcgc
     6241 gtggccaggg tccggctgat ccggaacagc gctctcgcct cgtgctcacg ctcgatggcc
     6301 acctgggctc gactccgctg ggccgcggcc agctgtccga ccgcgatgcc gaggacgagc
     6361 agcaggatca gattgagaag ctcacccggg tcggagacgg cgagggtgaa gttcggatcg
     6421 acaaacagga agttgtagag gaggaatgcg cccacggcgg tcgcgatcgc cgcgaagact
     6481 ccgaaataaa ccgcgctggc caggaccgcg aggaggtacg tggacgaggc gtccgggacg
     6541 ccgacccagc gctcgaggac cgcaatcagg gcggtggtgg ccgccagcag ggctgcgacg
     6601 gcgatcgccg ccttcatcgg tcccacgagg cgcgatccgc gagcggtcac cggtcaatgg
     6661 taggcctcgc accggcgatc agcggccgcc gcggcggtat ggtggccgtt ggcccccgtg
     6721 gcatactggc ccgactcgcg cggggcgtag cgcagcttgg tagcgcacct gaatggggtt
     6781 caggaggtcg ctggttc
//
LOCUS       DASYGU010000223         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1321099, whole genome shotgun sequence.
ACCESSION   DASYGU010000223 DASYGU010000000
VERSION     DASYGU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321099"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VGO32_06225"
     CDS             complement(<1..719)
                     /locus_tag="VGO32_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV7200381.1"
                     /translation="MPEPGTFLEVSVADAVKLADQRRIIDVRELVEWNEGHIPSATLV
                     PLADLPTTIETVVPDKSTALLIHCAVGARSRRASQYLAEIGYTDVLNIQGRMAEWKAL
                     GGAWEVPAQLLTDAERARYSRQLLIPEIGQEGQRKLLDSRVLLIGAGGLGSPAGLYLA
                     AAGIGTIGIVDDDVVDASNLQRQVLHTADRVGMPKTESAEIALKALNPDVTVIRHPFR
                     LDETNVDALVASYDVIVDGTD"
     gene            complement(719..1123)
                     /locus_tag="VGO32_06230"
     CDS             complement(719..1123)
                     /locus_tag="VGO32_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV7200382.1"
                     /translation="MDNLYRDFILEHYKNPHNAGVLDPHDLHAADSNPTCGDEMSMTL
                     RLTDDRVGIADVAFEGRGCAISQASASILTDTLRGMSLDEVRAINPKAVLDDLGVPIG
                     PARLKCALLGYKVLQGAVTGSTVEWPVEAEAV"
     gene            complement(1136..2398)
                     /locus_tag="VGO32_06235"
     CDS             complement(1136..2398)
                     /locus_tag="VGO32_06235"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEV7200383.1"
                     /translation="MAVRAEPAAIPGAFSGTERRADFPVFERQTSTGNRLVWLDSAAS
                     SPKPQVVIDSMADAYSHHYANVHRGIYELSEDATQRFEAARRKVAQFLGAPTDKEIVF
                     VRNATEAINLVAYSWGRANLRAGDVMVTTQLEHHANIVPWQQLTREVGAELRYVAITD
                     DGRLDLDDLDRHLAARPKLVAVAAVSNALGTINPIVDITERAHAAGALVLVDAAQAAP
                     HMPIDIGAIGCDFLAISGHKMLGPSGIGALWARRELLEAMPPFMTGGNMIIKVTLEGA
                     EWNEVPQKFEAGTPAIVEAIGLGAAIDYLTDLGMANVREHERYLFEHAWAALGEIPGV
                     RRLGPDDADIHAGVISFVFDAVHPHDMATILDHHGVAVRAGHHCAQPVMQRYDIPATT
                     RASFYVFNDLDDVEALAGAVRAAVTLFS"
BASE COUNT          365 a          820 c          923 g          418 t
ORIGIN      
        1 ttatccgtcc cgtccacgat cacgtcgtat gacgccacca gcgcgtccac gttcgtctcg
       61 tccaggcgga acggatggcg gatgaccgtc acgtcggggt tgagcgcctt gagggcgatc
      121 tccgcgcttt cggtcttggg catccccacc cggtccgcag tgtggaggac ttggcgctga
      181 aggttggacg cgtccacgac gtcgtcatcc acgatcccga tcgtcccgat cccggccgcg
      241 gcgaggtaca ggccggcggg cgagccgagg cccccggcgc cgatgagcag gacgcgggag
      301 tcgagcagct tgcgctggcc ctcctggccg atctcgggaa tgagcagctg gcgcgagtac
      361 cgggcccgct cggcatcggt caagagctgg gctggtacct cccaggcgcc accgagcgcc
      421 ttccactcgg ccatgcggcc ctggatgttc aggacgtcgg tgtagccgat ctcggccagg
      481 tactgcgacg cgcgccgcga ccgggcgccg accgcgcaat ggatgaggag cgcggtggac
      541 ttgtccggga cgacggtctc gatggtcgtt gggaggtcgg ccagcgggac gagcgtcgcg
      601 gaggggatat ggccctcgtt ccactcgacc agctcgcgga cgtcgatgat ccgccgctgg
      661 tcggccagct tgacggcgtc ggcgaccgag acttcgagga aggttccggg ctcgggcatc
      721 agaccgcttc ggcctcgacc ggccactcga cggtcgaacc ggttaccgct ccctggagca
      781 ccttgtagcc gagcagggcg cacttcaacc gggccggacc gatgggcacg cccaggtcgt
      841 cgagcacggc cttggggttg atggcccgca cctcgtcgag ggacatgccg cgcagggtgt
      901 cggtcaagat cgaggccgat gcctggctga tcgcgcagcc gcggccctcg aaggccacgt
      961 ccgcgatccc aacgcgatca tcagtcaggc gcagggtcat ggacatctcg tcgccacagg
     1021 tcgggttcga gtcggcggca tggaggtcat gggggtcgag cacgcccgcg ttgtgcgggt
     1081 tcttgtagtg ctcgaggatg aagtcgcggt acaggttgtc catcggtgct cgggctcagc
     1141 tgaacagggt gacggccgct cgaacggcgc cagccagggc ctcgacgtca tcgaggtcgt
     1201 tgaagacgta gaagctggcc cgggtggtgg ccgggatgtc gtagcgctgc atgaccggct
     1261 gggcgcagtg gtggccggct cggaccgcca ccccgtggtg gtcgaggatg gtggccatgt
     1321 cgtgcggatg gaccgcatcg aacacgaagc tgatgacccc ggcgtgaatg tccgcgtcgt
     1381 ccggcccgag tcggcggacg cccgggatct cccccagcgc ggcccatgcg tgctcgaaca
     1441 ggtagcgctc gtgctcgcgg acgttggcca tgcccaggtc ggtgaggtag tcgatggccg
     1501 cgccgaggcc gatggcctcc acgattgccg gcgtgccggc ctcgaacttc tgtgggacct
     1561 cgttccactc cgcgccttcg agcgtgacct tgatgatcat gttgccgccg gtcatgaacg
     1621 ggggcatggc ctccagcagc tcccggcgag cccagagcgc accgatcccg gaggggccga
     1681 gcatcttgtg cccgctgatg gccaggaagt cacagccgat ggcgccgatg tcgatgggca
     1741 tgtgcggggc cgcctgcgcc gcgtcaacca ggaccagggc gccggcggca tgtgcccgct
     1801 cggtgatgtc gacgatcggg ttgatcgtgc ccagcgcgtt gctcaccgcc gcgaccgcga
     1861 ccagcttggg cctggccgcc agatggcggt ccaggtcgtc gaggtccagg cgaccgtcat
     1921 cggtgatggc cacgtagcgc agctcggcgc ccacctcgcg ggtcagctgc tgccagggga
     1981 cgatgttggc gtgatgctcg agctgggtgg tgaccatcac gtcaccggcc cgcaggttgg
     2041 cccggcccca cgagtaggcg accaggttga tggcctcggt cgcgttgcgg acgaacacga
     2101 tctccttgtc ggtcggcgcg ccgaggaact gggcgacctt ccgacgggct gcttcgaagc
     2161 gctgggtggc gtcctcgctc agctcgtaga tcccgcgatg gacgttggcg tagtggtggg
     2221 aataggcgtc cgccatggaa tcgatcacga cctgcggctt gggcgacgac gcggcggagt
     2281 cgagccatac cagccggttg ccagtcgagg tctggcgctc gaagaccggg aagtcggcgc
     2341 gccgctcggt gccgctgaag gcaccgggaa tggcggctgg ttcggcgcgg acggccatcg
     2401 gtcctccctc ctctgcgcta atgggacgcc gcttcggcgg gctgcggggc gacctcgtcg
     2461 ccgatgccgg ccggcaggcc aacctgctcg cggacccagt cgtagccgtt ggcctcgagc
     2521 tggtgc
//
LOCUS       DASYGU010000224         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1269929, whole genome shotgun sequence.
ACCESSION   DASYGU010000224 DASYGU010000000
VERSION     DASYGU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269929"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGO32_06240"
     CDS             complement(<1..261)
                     /locus_tag="VGO32_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200384.1"
                     /translation="MRRLSAAAGLLVAALTLIAVIVPGAGPASAGGRPLRYLAGEAGT
                     LDPAFISSAGDVQLLLQLYAGLTRIDDDGAVYASLAESWTLTD"
     gene            337..873
                     /locus_tag="VGO32_06245"
     CDS             337..873
                     /locus_tag="VGO32_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200385.1"
                     /translation="MEQTHEHRFRHQPAGSLTLRQFVESLRRWTPEMRQIAQIALTRT
                     DAERAQYLQGYHHGHEERPAASLDSFVRWAYSDPRIDAVPSLLAEIERPDSAYEGALL
                     HEAEVLVRTRGEGRTPAQRVADYLGLLGTESSERVRLTISSLLPIGTPEPIVTATIRQ
                     MRRSWLEIYGDDPFFAED"
     gene            848..3148
                     /locus_tag="VGO32_06250"
     CDS             848..3148
                     /locus_tag="VGO32_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEV7200386.1"
                     /translation="MTRSLPRTDRAGILPLLIAAVLATQAASFAAPAVVRGHSILLDA
                     DPAPNSVIPASPSQLSLFFSEAVDPRSISIEVVNSEQHPVVGVGAPSVDSTGQVVRAT
                     LPKLDPDTYTVEYSVVSAVDGHPSASLYAFVLDPTGTEPPPGLPLPSDTAAPPDPVAV
                     AARWLTTVAGLLLLGAVMAWLFHRRWIGAEDRVPVPWVPLAALAGIGSVALVGYVARS
                     AAGAFSHGHGQPAGLPFDPLAPFGWTAFAIAMRIALIGLVVAGVVAATAGSSAGRRRL
                     MVVGGATVVALFGMSLTGHAAALGGPIWAMVDAAHLIAIAAWLGALPALAVLARRSGT
                     GRAAFAVHARVALVAAPLVILTGLANSPLVVDDSRELVASGYGNLLLAKTLLVSVAVG
                     LGSASYFLARGGGRRRLAAVAGGEVVVAIMAVVVGVSMVSIQPATDRPPSTVDPRLGV
                     AHVYGEGGGSSVHGIVDLPEPGVQSYSFSVADPETGAGREDVAEVTVTFVPPAGSGLV
                     SNTELAEPTQQPWIWTLRGAFTPVVGNWELEIAVHRGRLVEDRMKMPLAVRQVLQSTP
                     LPPATTGSQILGVVAAPTAGLPAGAAGWIVPILLLGGGAVALAVERAQSPGPVRGRRL
                     IRGIRVAVLGAAVVIGVSLVARDLVAAANRAPDEWAEAANPLADDPDAAAAGEELYRA
                     NCQSCHGPEGAGDGPAADDLSRPPADLAAIVPDRLDGELGWTIAAGVAGTQMPAFGTT
                     LLDGERWELVSYLRSRWPLAAGDDAP"
     gene            3145..3516
                     /locus_tag="VGO32_06255"
     CDS             3145..3516
                     /locus_tag="VGO32_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200387.1"
                     /translation="MRTLRRAGAATMLVIAAFMAGCQPATCPDPSITLAATVTADSMD
                     PSALTACKGQEVTLEVTSETDGILHIHGYDEQVPATPLEPGEERTLEFTADVAGQFVI
                     ELHARDGETETEIGILTVNEP"
     gene            3506..5026
                     /locus_tag="VGO32_06260"
     CDS             3506..5026
                     /locus_tag="VGO32_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200388.1"
                     /translation="MSRRPGRPAVVTAATILALAATMVVPGAALGHALGSTFPLPVPL
                     WLYLAAAAVAVAASFVVAVVVVKPPGDQPRYRTWPVSPRTARIGGFVLRILGLVWWYG
                     AIAVGFALGGITYIPAVLFWIGIWVGVPIAAAVLGNPWPSLSPFRTTYDAVDALVRWI
                     AGGRRLDLGVHIPNLGRGPAVVLLFVFLIMELVWPDRLNPVSLAAALAVYTVATVLGM
                     VIFGSVTWLRSFELFEILNGWFGRVGPAGRRTERAALCTGCTDGCSPEHCVDCAECMA
                     ARDDDDQTVELRWWITGLTERTPGGWGDAAFIILALAGVTFDGLQETGLGARLLSFLF
                     TPLSTVFGPLAASYLSPSILLLGLFVFFLGAFALAVFLTQRIGSDAAAGTSRSLGHVA
                     GTYAPTLLPIAAGYLIAHYLTLLIQGIIWLPLLIIDPTALAPVLDWIPTAFIWYLSVA
                     AIVGGHIAAVVLAHRLALRDHAKRPILAGLPLVLLMIGYTVLSLWIIAQPIVVEPV"
     gene            5031..5756
                     /locus_tag="VGO32_06265"
     CDS             5031..5756
                     /locus_tag="VGO32_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV7200389.1"
                     /translation="MELSLALAFAAGVVSFITPCVLALVPVYLAVLADAAVVAPAPAV
                     AEAPPAVGGRRPMLVAAALFTLGFSTVFVLLGISVGLIGMALFREPLVRQLAGVVVIG
                     LGLLMTGMFGPVLDRLPNPASALPPATARETRALALGAVVAIGWTPCIGPVLGAILAM
                     GASTQDVVSATFLLIAYSLGLALPFLLTAAFLPRARPALRWLRAHERPIRIAAGLAVA
                     AVGVLIVLDAFTRLAGLFGEFFL"
     gene            complement(5772..>6026)
                     /locus_tag="VGO32_06270"
     CDS             complement(5772..>6026)
                     /locus_tag="VGO32_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV7200390.1"
                     /translation="AVFGTAVDKRGPAIGGLAIGLAVAVDIFFGASLTGAAMNPARWF
                     GLAVAAGDYTNWYVWWIGPFLGAVVVALLYRFLLAPPDEE"
BASE COUNT          727 a         2225 c         2080 g          994 t
ORIGIN      
        1 atcggtgagc gtccagctct cggccagcga tgcgtagacc gcaccgtcgt cgtcgatccg
       61 ggtcaggccg gcatacagct gcaggaggag ctgcacgtcc cccgcgctcg agatgaatgc
      121 cggatcgagc gtccccgcct caccggccag gtagcgcagc ggacgaccgc cagcggaggc
      181 gggtccggca ccgggaacga tgaccgcgat gagggtgagg gccgcgacga gcagcccggc
      241 cgccgcgctc aggcggcgca tgccagggtc aagggcggct ccgggaccag gccggcgtcg
      301 tcgttcggcg gcgcacggcc ggtagtatag gtgcccgtgg agcagaccca tgaacaccgg
      361 ttccggcacc agccggcagg gtcgctcacc ctccgtcaat tcgtcgagtc gctccggcgc
      421 tggacgccgg agatgcgaca gatcgcccag atcgccctga cccggaccga tgccgaacgc
      481 gcgcagtacc tgcagggcta ccaccacggg cacgaggagc ggcccgcggc gtcgctcgac
      541 tcgttcgtcc ggtgggcgta ctccgatccg cggatcgatg ccgtcccgtc cctcctggcc
      601 gagatcgagc gccccgacag cgcgtacgag ggcgcgctcc tccacgaggc ggaggtcctc
      661 gtccgaaccc ggggcgaggg tcggaccccc gcccagcggg tggctgacta cctcggcctg
      721 ctcggcaccg agagcagcga gcgggtcagg ctcacgatca gctcgctcct cccgatcggc
      781 accccggagc cgatcgtgac cgcgacgatt cgccagatgc gccgatcctg gcttgagatc
      841 tacggcgatg acccgttctt tgccgaggac tgaccgggcc ggcattctcc cgctgctgat
      901 cgcggctgtt ctcgcgaccc aggctgcgtc cttcgccgcg ccggccgtcg ttcgcggcca
      961 ctcgatcctg ctcgatgccg atccggcacc caacagcgtc atccccgcct cgccctcgca
     1021 gctgtccctg ttcttcagcg aggcggtgga cccgcgctcg atcagcatcg aggtcgtcaa
     1081 ctcggagcag cacccggtcg tgggcgtcgg ggcgccgagc gtggacagca ccggccaggt
     1141 cgtgcgcgcc acgctgccca agctcgaccc cgacacctac accgtcgagt actcggtcgt
     1201 gtccgctgtg gacggccatc catcggcctc gctgtacgcg ttcgtgcttg atccgaccgg
     1261 caccgagcca ccgcccgggc tcccgctgcc ctctgacacg gccgccccac ccgatccggt
     1321 ggctgtcgcc gctcgttggc tgaccacggt cgccggcctc ctcctgctgg gcgcggtcat
     1381 ggcgtggctc ttccaccggc gctggatcgg tgccgaggac cgcgttcccg tcccgtgggt
     1441 cccgctcgcg gcgctcgcgg ggatcggctc ggtggccctc gtcggatacg tggcgcgctc
     1501 cgcggccggg gccttcagcc acggccatgg ccaaccggcc ggccttccct tcgatccgct
     1561 cgcgccgttc ggctggaccg ccttcgcaat cgcgatgcgg atcgcgctca tcggcctggt
     1621 cgtcgcaggc gtggtcgccg caaccgccgg atcgagcgcc ggccggcgtc ggctgatggt
     1681 cgtgggcggc gcaaccgtgg tcgccctgtt cggaatgagc ctgaccggtc atgccgctgc
     1741 cctcggcgga ccgatctggg caatggtcga cgctgcccac ctgatcgcga tcgcggcctg
     1801 gctcggcgcc ctgcccgccc tggcggtcct ggctcgccgg tcggggaccg ggcgtgccgc
     1861 gttcgcggtc cacgcccgtg tggcgctggt ggccgccccg ctcgtgatcc tgaccggcct
     1921 ggccaattcg ccgctcgtcg tcgacgactc gcgagagctg gtggcatccg ggtacgggaa
     1981 cctgctgctg gccaaaacgc tgctggtctc ggtggccgtc ggcctcgggt cggcgagcta
     2041 cttcctcgcc cggggcgggg gccggcggcg actggcggcg gtggccggtg gcgaggtcgt
     2101 ggtcgcgatc atggccgtcg tggtcggcgt gtcgatggtc agcattcagc ccgcgaccga
     2161 ccgcccaccg tcgaccgtcg atccgcgact gggcgtggcg cacgtctacg gggagggcgg
     2221 cggctcgagc gtgcatggca tcgtggatct gcccgagccc ggggtgcagt cctactcgtt
     2281 ctcggtggcc gaccccgaga ccggcgccgg tcgcgaggac gtggccgagg tgacggtcac
     2341 cttcgtgcca cccgccggca gcgggctggt gtccaacacc gagctcgctg agccgactca
     2401 gcagccgtgg atctggaccc tgcgcggcgc gttcacaccg gtcgtcggga actgggagct
     2461 ggagattgcg gtgcatcgcg gccggctcgt cgaggaccgg atgaagatgc cgctcgccgt
     2521 acgccaggtc cttcagtcga cgccgctccc gccggcaacg accggcagcc agatcctcgg
     2581 tgtcgtcgcc gccccgacgg ccggcctccc ggccggagcc gcgggctgga tcgtccccat
     2641 cctgctgctg ggcggcggag ccgtcgcact cgcggtggaa cgagcgcaat cgccgggacc
     2701 ggttcgaggc cgtcgcctga tccgcgggat ccgggtggcc gtgctcggcg cggcggtcgt
     2761 tatcggcgtc tccctggtgg cgcgggacct ggtggccgcg gccaaccgcg cgcccgacga
     2821 atgggcggag gcggcgaacc cgctggccga cgaccccgat gcggcggcgg ccggtgaaga
     2881 gctgtaccgg gccaactgcc agagctgcca tggccccgag ggcgcgggcg acggacccgc
     2941 ggctgacgac ctgagccgac caccggcgga tctcgccgcg atcgttccgg atcgccttga
     3001 cggcgagctg ggatggacga tcgcggccgg cgtggccggc acccagatgc cggcctttgg
     3061 cactaccctg ttggacggcg agcgctggga gctggtgagc tacctccgct cgcgctggcc
     3121 gcttgccgca ggagacgatg ccccgtgaga acgcttcgcc gtgccggagc cgcgacgatg
     3181 ctggtcatcg ccgccttcat ggcaggatgt cagcccgcca cctgccccga tccgtcgatc
     3241 acgctggccg ccacggtgac ggcggattcg atggatccat cggcgctgac cgcctgcaaa
     3301 ggacaggaag tgaccctcga ggtcacgagc gagaccgatg gaatcctcca catccacggc
     3361 tatgacgaac aggttcccgc gacccccctc gaaccgggag aggagcgcac cctcgagttc
     3421 accgctgacg tcgcggggca gttcgtcatc gagctccatg ctcgcgacgg cgagacggaa
     3481 accgagatcg gcatcctgac cgtcaatgag ccgtaggccg gggcggcccg ccgtcgtcac
     3541 ggcggccacc atcctcgctc tcgccgcgac gatggtcgtg ccgggggccg ccttgggcca
     3601 cgccctgggt tccacgttcc cgctccccgt cccgctctgg ctctacctcg ccgcggcggc
     3661 ggtcgccgtc gccgcgtcgt tcgtggtggc cgtggtggtc gtcaagccac cgggagatca
     3721 gcctcgctac cgcacctggc cggtctcacc gcggaccgca cggatcggag gcttcgtgct
     3781 ccgcatcctc ggtctcgtct ggtggtacgg cgcgatcgcg gtcgggttcg cgctgggcgg
     3841 aatcacgtac atcccggccg tcctgttctg gatcggcatc tgggtgggtg tcccaatcgc
     3901 cgccgccgtg ctcggcaacc catggccctc gctcagcccg ttccgtacga cgtacgacgc
     3961 cgttgatgcc ctcgtgcgct ggatcgcggg cgggcggcgc ctggacctcg gcgtgcacat
     4021 tccgaacctc ggccgcgggc cggccgtggt cctgctcttc gtgttcctga tcatggagct
     4081 ggtctggccc gaccgcctga accccgtgtc gctggccgcc gcactggccg tctacacggt
     4141 cgcgaccgtg ctcgggatgg tcatcttcgg gagcgtgacc tggcttcgat ccttcgagct
     4201 gttcgagatc ctgaacggct ggttcggtcg ggttggtccc gccggccgtc ggacggagcg
     4261 cgcggcgctg tgcaccggct gcaccgatgg ctgctcaccg gagcactgcg ttgactgcgc
     4321 cgagtgcatg gccgcgcgcg acgacgacga ccagaccgtc gagctgcggt ggtggatcac
     4381 cggcctcacc gagcggacgc ccggcggatg gggtgacgcc gcgttcatca tcctggccct
     4441 ggcgggagtc acgttcgatg gcctgcagga gaccgggctc ggggcccgcc ttctctcatt
     4501 cctgttcacg ccgctgtcca ccgtgttcgg cccgttggcc gcgagctacc tgtccccgag
     4561 catcctgctc ttgggcctgt tcgtcttctt cctgggggcg tttgcgctgg ccgtgttcct
     4621 gacccagcgc atcggctcag atgcggccgc cgggacttcc cgatcgctcg gccacgtggc
     4681 cggcacctac gccccgaccc tgctcccgat cgccgccggc tacctcatcg cgcactacct
     4741 gaccctcctc atccagggaa tcatctggct cccgctcctg atcatcgatc ccacggccct
     4801 ggccccggtc ctcgactgga tcccgaccgc gttcatctgg tacctgtcgg tcgccgcgat
     4861 cgtgggcggg cacatcgccg ccgtggtact cgctcaccga ctcgccctcc gcgaccacgc
     4921 caagcggccg atcctcgccg gcctgcccct cgtcctgctc atgatcggct acaccgtcct
     4981 gtcgctctgg atcatcgccc agccgatcgt ggtcgagccg gtctgagccg gtggaactga
     5041 gcctggcgct ggccttcgcg gccggcgtgg tctcgttcat cacgccctgc gtcctggccc
     5101 tcgttccggt gtacctcgcg gtgctggccg atgccgcggt ggttgcgccg gcgcctgccg
     5161 tcgccgaggc gccaccggcg gttggcggcc gtcgcccgat gctcgtcgcg gccgccctgt
     5221 tcacgctcgg gttcagcacc gtgttcgtcc tgctcgggat ctcggtcggc ctgatcggga
     5281 tggcgctctt ccgggagccc ctcgttcgtc agctggccgg cgtggtcgtc atcgggctgg
     5341 gactgctcat gaccgggatg ttcgggccgg ttctcgaccg tctgccgaac ccggcctcgg
     5401 ccctgccgcc cgccaccgcg cgcgaaacgc gggccctggc gctgggagcg gtggtcgcca
     5461 tcggctggac cccgtgcatt ggtccggtgc tgggcgcgat cctggcgatg ggcgcctcca
     5521 cccaggacgt ggtgagcgcc accttcctgc tcatcgccta ctcgctcggg ctggcgctcc
     5581 cgttcctgct caccgccgcc ttcctgcccc gtgcccggcc ggcgctgcgc tggctgcgcg
     5641 cccacgagcg cccgatccgg atcgcggccg ggctggctgt cgcggcggtc ggggtcctga
     5701 tcgtgctgga cgcgttcacc cgactggccg ggttgttcgg cgagttcttc ctctagccct
     5761 cgagccttcc tctactcctc gtcgggcggg gccagaagga agcggtacag cagcgcgacg
     5821 acgacggcgc cgaggaacgg tccgatccac cacacgtacc agttcgtgta gtcgccggcg
     5881 gcgaccgcca gcccgaacca gcgggccggg ttcatggccg caccggtgag cgacgcgccg
     5941 aagaagatgt ccaccgcgac cgcgaggccg atggcgagcc cgccaatagc cgggccgcgc
     6001 ttgtcgaccg cggttccgaa gaccgc
//
LOCUS       DASYGU010000225         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_396170, whole genome shotgun sequence.
ACCESSION   DASYGU010000225 DASYGU010000000
VERSION     DASYGU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396170"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VGO32_06275"
     CDS             <1..1188
                     /locus_tag="VGO32_06275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7200391.1"
                     /translation="ISAEPADPSDETLTNAVLAAAAGYRFGDIEVPVTIARALEQMGY
                     VTPTEVQARAIPPLRAGGDFIGQAQTGTGKTAAFGIPIVEQVDPSDKSVQALVLTPTR
                     ELCQQVTDEIGRIGQFRNVKTVAIYGGAAMDRQITALQRGAQVVVGTPGRVLDLIRRR
                     ELRLDSVRIVILDEADRMLDMGFIVDVETILARCPRQRQTALFSATMPYAILRICDRF
                     MRRDAEHVAVKPELRTVETTRQLVYFVAEQDKVAALVELVDQFGFDRLLVFRHTQIGV
                     DRLTTSLKRRGRNVAALHGGLSQRDRERTLAAFKAGDIQFLVATNVASRGLDIVDLPY
                     VLSFDIPEDADTYIHRVGRTGRAGRTGTAITFVAEWDLDAWQSIKAEVGMDVEEGELR
                     LYR"
     gene            1185..1892
                     /locus_tag="VGO32_06280"
     CDS             1185..1892
                     /locus_tag="VGO32_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200392.1"
                     /translation="MTEPTGPPVDPAALRPQLATEAGVRINSPHWSFEPYWIGARLMA
                     RVADGQVRVTNADGDPVDDFYRDLASVLAGSVDAEQAVLDGIWTAQPFLGHGSAAERW
                     SQAVAEESGDLPEDGPDPADLETRRAFVVVDVIELDGEFLGELPYQERRRLLAGLISE
                     SVRLRVTPSVKMPLGSWFHAWQENGFSRAVAKHANSRYQPGTATADWVVITIEPDRAP
                     GATGLFWKGRKKREITR"
     gene            1889..3178
                     /locus_tag="VGO32_06285"
     CDS             1889..3178
                     /locus_tag="VGO32_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV7200393.1"
                     /translation="MTVASALDARGRSAVRGIGQADLMVGIPSFGNAETIGYVVRAAM
                     AGMVQYFPELKPVLVNADGGSKDDTPQVAVSTESPEYIEKMILVRPPHRLRRVAVTYR
                     GPSGKGSAVRALLEVAAELKVEALVLVDSDLRSIVPEWIELLAGPILKGGYDYVAPLY
                     ARHKFDGTITNHIAYPLTRALHGRRMRQPIGGEFAISRDLVHALLEYTDWDEATARFG
                     IDIWMTHHALAGGFAVCQTRLGAKIHDPKDPASDLGPMFRQVVGTLFRMAGEQSESWL
                     GIRGSQAVPEYGFERVVLPEPITVNQPKLVDAFETARLAQGAVWKRILAGEQLERVLA
                     VPSDVPETFAFPSELWIRCLYDALLAFQRPGLDREALLAGLTGLYFGRVAGFINEVKD
                     LADDQSERLVEAQAREFEDLKPWLARAWQAQAGGESA"
     gene            3178..>3949
                     /locus_tag="VGO32_06290"
     CDS             3178..>3949
                     /locus_tag="VGO32_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7200394.1"
                     /translation="MGRRGPYRILMPLANPRTARDLVRIGSHLAPRRPTEITALGIVE
                     VPEGVSLAEGASRAREARRLLQRVPDFRDDPSVEVRTAVRIGRQASEGIIEAVAEIGA
                     DLVIFGWGGPPRNEARRAVFSTTIDEVAREAPCDIAVVKQRGLGAVESILVPVRGGPN
                     AELAVSLATDLARGFRAELVVLHVVPPGGGLEAIERERTALAALVKRAARGVSVRDLV
                     VEAPTVRGAILAEAANHQLVVVGASAQPTGIGSGRRLFG"
BASE COUNT          569 a         1398 c         1402 g          580 t
ORIGIN      
        1 atctccgcag agcccgcgga cccgtccgac gagacactca ccaacgcggt cctggccgcc
       61 gctgccggct accggttcgg cgacatcgag gtcccggtca cgatcgcccg ggccctcgag
      121 cagatgggct acgtgactcc caccgaggtt caggcccgcg ccatcccgcc gctgcgcgcc
      181 ggaggcgact tcatcggcca ggcgcagacc ggaaccggca agaccgcggc cttcggcatt
      241 ccgatcgtcg aacaggtgga tccgtcggac aaatcggtcc aggccctggt cctgaccccc
      301 acccgcgagc tgtgccagca ggtcaccgac gagatcgggc gcatcgggca gttccggaac
      361 gtgaagacgg tcgccatcta cggcggcgcg gcgatggacc gccagatcac cgcgctccag
      421 cgcggcgcgc aggtcgtggt gggcaccccg gggcgggtgc tcgacctcat ccggcggcgc
      481 gagctccggc tcgactcggt ccggatcgtg atcctcgacg aggccgatcg gatgctggac
      541 atggggttca tcgtcgacgt cgagacgatc ctcgcccggt gcccgcggca gcggcagacc
      601 gcgctgttca gcgcgaccat gccgtacgcc attctccgca tctgtgaccg cttcatgcgg
      661 cgcgacgcgg agcacgtggc ggtcaagccc gagctgcgca ccgtggagac cacgcgccag
      721 ctcgtctact tcgtcgcgga gcaggacaag gtcgccgccc tggtcgagct ggtcgaccag
      781 ttcgggttcg atcggctgct cgtcttccgc cacacccaga tcggggttga ccggctgacc
      841 acctcgctga agcgtcgagg gcgcaacgtc gcggcgctcc atggcgggct gtcgcagcgg
      901 gaccgggagc ggacgctggc cgcgttcaag gccggcgaca tccagttcct ggtcgcgacc
      961 aatgtcgcca gccgcggcct cgacatcgtg gatctgccgt acgtgctcag cttcgacatc
     1021 cccgaagacg ccgacaccta catccaccgc gtgggccgga ccggccgagc gggccgaacg
     1081 gggaccgcga tcacgttcgt cgccgagtgg gacctcgacg cctggcagag catcaaggcc
     1141 gaagtcggga tggacgtcga ggaaggcgag ctccgcctgt atcgatgacc gagccgaccg
     1201 gtccgcccgt cgacccggca gccctccgcc cccagctggc gaccgaagcg ggggttcgga
     1261 tcaacagccc gcactggtcg ttcgagccct actggatcgg cgcacggctg atggcccgag
     1321 tcgccgacgg gcaggttcgg gtgaccaacg ccgacggcga cccggtcgac gacttctacc
     1381 gcgacctcgc gtcggtcctc gccggatcgg tggacgccga gcaggcggtg ctggacggca
     1441 tctggaccgc gcagccgttc ctgggccacg gttcggccgc cgagcgctgg tcgcaggcgg
     1501 tggccgagga gagcggcgac ctgcccgagg acggacccga tccggcggac ctggagaccc
     1561 ggcgggcgtt cgtggtcgtc gacgtgatcg agcttgacgg cgagttcctc ggcgagctgc
     1621 cataccagga gcggcggcgg ctcctggccg ggctgatcag tgagagcgtc cgattgcggg
     1681 tcacgccctc ggtcaagatg ccactcgggt cctggttcca tgcctggcag gagaacggat
     1741 tcagccgggc ggtggccaag cacgcgaact cgcggtacca gcctgggacc gcgacggcgg
     1801 actgggtcgt gatcaccatc gagcccgacc gcgcgccggg ggcgaccggg ctgttctgga
     1861 agggccgcaa gaagcgcgag atcacgcgtt gacggttgcc tcggccctcg atgcgcgcgg
     1921 ccggtccgcg gtgcgtggga tcggccaggc ggacctcatg gtcgggattc cgagcttcgg
     1981 gaatgccgag accatcggct atgtcgtgcg ggcggcgatg gcgggcatgg tccagtactt
     2041 cccggagctc aagccggtcc tcgtcaacgc ggacggcggc tccaaggacg acacgccgca
     2101 ggtggcggtc agcaccgaga gccccgagta catcgagaag atgatcctgg tccgcccgcc
     2161 gcatcggctg cgccgggtgg ccgtcaccta ccgcggcccg agcggcaagg gatcggcggt
     2221 gcgcgccctg ctcgaggtcg cggccgagct gaaggtcgag gccctggtgc tggtcgactc
     2281 cgacctccgc tccatcgtgc cggagtggat cgagctcctg gccggaccga tcctcaaggg
     2341 cggctacgac tacgtcgccc cgctgtacgc ccgccacaag ttcgacggca cgatcaccaa
     2401 ccacatcgcc tatcccctca cccgggcgct ccatggtcga cggatgcgcc agccgatcgg
     2461 tggcgagttc gcgatcagcc gtgacctagt ccatgccctg ctcgaataca cggactggga
     2521 cgaggcgacc gcccgtttcg ggatcgacat ctggatgacc caccatgcgc tggccggcgg
     2581 gttcgccgtg tgtcagaccc ggctcggggc gaagatccac gatcccaagg acccagcctc
     2641 cgacctgggg ccgatgttcc gccaggtggt gggcaccctg ttccggatgg ccggcgagca
     2701 gtccgagtcc tggctcggca tccgcggttc gcaggcggtg cccgagtacg gcttcgaacg
     2761 ggtcgtcctg cccgagccga tcacggtcaa ccagccgaag ctggtcgatg cgttcgaaac
     2821 cgcgcgcctg gcccagggcg cggtgtggaa gcggatcctc gccggcgagc agctcgaacg
     2881 agtcctcgcc gtcccttcgg acgtgccgga gacgttcgcc tttccatccg agctgtggat
     2941 ccgctgcctg tacgacgcct tgctcgcctt ccagcggccg ggactcgacc gcgaggccct
     3001 gctggccggc ctgaccggcc tctacttcgg gcgggtcgcc ggtttcatca acgaggtcaa
     3061 ggacctggcc gatgaccagt ccgagcggtt ggtcgaggcc caggcccgcg agttcgagga
     3121 tctcaagccg tggctggccc gggcatggca ggcccaggcc gggggcgagt ccgcctgatg
     3181 ggccgccgcg gtccgtaccg gatcctcatg cccctggcca acccgcgcac cgcgcgcgac
     3241 ctggtccgca tcggctccca tctcgcccct cgccgtccga ccgagatcac ggccctgggc
     3301 atcgtggagg tgcccgaggg cgtctcgctg gccgaggggg cgagccgcgc ccgcgaggcg
     3361 cgtcgactcc tccagcgcgt tcccgacttt cgcgacgacc cgtcggtcga ggtccggacc
     3421 gcggtgcgga tcggccgcca ggcatccgag gggatcatcg aggccgtggc cgagatcggc
     3481 gccgacctgg tcatcttcgg ctggggcggg cccccgcgga atgaggcccg gcgcgcggtg
     3541 ttctcgacga ccatcgacga ggtggcccgc gaggcgccct gcgacatcgc ggtcgtgaag
     3601 cagcgcgggc tcggcgccgt ggagtcgatc ctggttcccg tccgcggcgg cccgaacgct
     3661 gagctggcgg tctccctggc caccgacctg gcgcgcgggt tccgcgccga gctcgtggtc
     3721 ctgcacgtcg tgccaccggg cggtggcctg gaggccatcg agcgggagcg gaccgcgctc
     3781 gccgccctgg tcaagcgcgc cgcacggggc gtgtcggtgc gcgacctggt ggtggaggcg
     3841 cccaccgttc ggggggccat cctggccgag gccgcaaacc accaactggt cgtcgtgggc
     3901 gcatccgccc agccgaccgg catcggctcg ggtcggcgac tgtttggcc
//
LOCUS       DASYGU010000226         9800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1835891, whole genome shotgun sequence.
ACCESSION   DASYGU010000226 DASYGU010000000
VERSION     DASYGU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9800
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835891"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..518)
                     /locus_tag="VGO32_06295"
     CDS             complement(18..518)
                     /locus_tag="VGO32_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV7200395.1"
                     /translation="MKAIVVYESHWGNTEAVARAIASGIGPEAEAMTTDQATSAAAAD
                     ASLIVAGAPVIAFGLPRDAMRDQIAAGGEKAPRPPDVSHPLLRTWLDELPVSEGWYAA
                     FETRIWWSPRGATGAIEAKLRKAGYRRAGKSERFIVDGAYGPLKDGELERARAWGTSL
                     ARAAGF"
     gene            complement(623..3160)
                     /locus_tag="VGO32_06300"
     CDS             complement(623..3160)
                     /locus_tag="VGO32_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV7200396.1"
                     /translation="MIRAAASPWARSAREVCASLDVDPATGLSDADAAVRLAENGPNE
                     LEAVPGPSILRALVDALREPFVLMLIGAAVLAIILGEVRDGILILIGVVPIVAADVAT
                     SYRAERALEVLRQANAPRARVRRDGEAVQIPAREVVVGDLALLAVGDVIPADLRVTTS
                     HALLVDRSTLTGESLPEPASVEADSSDAALSERRAMAYAGTNVVGGLGEGVVVSVGQT
                     TEVGRIASSLAAHARPRSPLERELARFVRILLVVALGLVVITVGLGFLRGNPAGENIL
                     AGVAAAIAAIPEEPPILLAVILGLGAYRLLRRDVLVRRLNAQETLGAVDFILTDKTGT
                     LTLNRLSVAAVRTPVGTAVGVERARILGEALRAEAEAWHAAGTGRTGAFARAIHDALA
                     DEDLVPELNPETLLDASPPTDLRPYAMTRCYDEQGLRDLALGAPEAVLELVRADGGLG
                     PAQGDLGGWERLIVEEADRGGRLLLLAARSEGAHWQPTATLVFADPLRPEIPAAVETA
                     LAAGIQVVMVTGDHPTTARAIAREAGLPGDIVLTGAEIQAMNPDALAARIRDLQIVAR
                     ATPADKLRLVEAAAASARTVAVTGDGVNDAPALHRADVAVAMGSGTAVAREAADLVLG
                     DDSFATLMDALREGRRMIANVQKGLVFLLSTHVALLGYVLLATVVGISLPLLPIQILW
                     MEFFIDISAAVAFEREAEEPNAMSVRPRPRDQPLLDRRLLVGICLAGAFTVASALALV
                     LSVSGDQEHANWVAFTTLVVAQLVRANANRTLGSSLLRLRPNLLLAGMGVLWLAVQAA
                     IPYIPPLADAFRASPLSATEWAAIAVIALTPAALAEVMRRRGRTWVA"
     gene            3423..4697
                     /locus_tag="VGO32_06305"
     CDS             3423..4697
                     /locus_tag="VGO32_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7200397.1"
                     /translation="MRAVLPRRAVAEIRDGHTIYIHGGAATPTALLTALAERGPKLKG
                     VTTVGLHLEGPCPHLAPELAGHIRHRALFIGPTARDAVNEGRADYVPIFLSDIPLLIS
                     RGILSLDCAFINVSPPDAHGYCSLGPSVDATLAAVRSARTVIAQINSAMPRTLGDSFV
                     HVREIDLMVEVAQPPHEHPAAEIGETERQIGALVADLIPDGATLQLGIGSIPAAVGAA
                     LGDKTDLGIHTELFTDTVVDLVEAGVVTGAAKEINTGKIVSSFVLGTPRLYRFIDNNP
                     MVEMRPVDYTNDTAVIRRFRKMVAINSAIEVDLSGQVVADSIGRRLYSGVGGQMDFIR
                     GAALAEEGRAIIALPSTAAGGAVSRITASLQPGAGVVTTRAHVRTVVTEWGVAELFGR
                     SLAERAHALIQIAHPAFRDELLRAAGQMHLDH"
     gene            4770..5261
                     /locus_tag="VGO32_06310"
     CDS             4770..5261
                     /locus_tag="VGO32_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200398.1"
                     /translation="MARDDITVRWVRAEDAARLEAFYSALTDDSRAARFHGACRGISH
                     ERAAHDSTADHHHRDGFVAEYEGRIVGHLVLEPMRAGAEEVAVAVADDLQHHGVGSLL
                     MAAAVASARLRGVRHLQAWIRPDNEAMRRLFTGTSRPTHLIAWDGYDARYELDVPPFP
                     HAA"
     gene            complement(5301..6116)
                     /locus_tag="VGO32_06315"
     CDS             complement(5301..6116)
                     /locus_tag="VGO32_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7200399.1"
                     /translation="MLSSISIESGERPEQDVWRALANPLRRQLLDLLGDGPKTTGELA
                     GAIPTVTRFAVMQHLGVLTEAGLVVVRRRGRQRFNHLNPVPLRRWYERWVVPFADRSA
                     AEMLALERHLASSEGGPSMAVLVDDVRVVRIETELRFRATPERVFRAVFDETLEWFPH
                     SYGDTRTRAVLNEPRVGGASYEDWGDGMGYLYGHVTVYDRPNRFATRGRVMPGTILDS
                     EYELEADGEETVLKASKIVVGPMTDEEAESIHTFGDIARFEAPLRALVEAVAS"
     gene            6315..6584
                     /locus_tag="VGO32_06320"
     CDS             6315..6584
                     /locus_tag="VGO32_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200400.1"
                     /translation="MTSPITATLAAHLADLAAFGLAISVYGIAGESNPIAYQLFIVGG
                     IPLLVAVKVAGSLVAALIVSRRRRWLPLAAGSGLLGAVTALMVLL"
     gene            complement(6859..7545)
                     /locus_tag="VGO32_06325"
     CDS             complement(6859..7545)
                     /locus_tag="VGO32_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEV7200401.1"
                     /translation="MDSDQSADEALTDTPAPESDGRTRPASEFLPLDRFNAFTDGVFA
                     IAITLLVLELTVPALEAPLLPTLAEQWHEFLGYLISFVFIGGIWVAHAGMTKLMREAD
                     AIAYGIDLLMLLFVGLLPFSTELMVTHLEGPDVTAAVVIYGANLLVASSTLSLLMFYI
                     ASERSLVLDSVADDELDAKVRRRWSIIVLNVVAIAVAIVLPIAAVGLYLITTIVGLLL
                     PLVRLRRRKR"
     gene            7700..8107
                     /locus_tag="VGO32_06330"
     CDS             7700..8107
                     /locus_tag="VGO32_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200402.1"
                     /translation="MTAAILVLALGLVAACTPASDQPSAEQALCDALATFRTSVRAIA
                     DLSPTTASIEDLQAGVDSAQEAWGGVQDAAEALGEADTSALETAWTDLADQISNFSTD
                     IPLREAIGEITAGVDEVQATYDEIRNGLGCEGS"
     gene            8441..8517
                     /locus_tag="VGO32_06335"
     tRNA            8441..8517
                     /locus_tag="VGO32_06335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8475..8477,aa:Val,seq:gac)
     gene            8568..9146
                     /locus_tag="VGO32_06340"
     CDS             8568..9146
                     /locus_tag="VGO32_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7200403.1"
                     /translation="MRVAVSPEQIRAAFEAVKDDPSFDESRGLWERGRPPVEMIQAMC
                     LRPEILRGFAGFGNAVYPGGLLERRVKELVIITASQTNECQFCTASHVDLVGIADIIQ
                     EPLVVIDDTESLAPRERLAVEYTRAAMADSNAIPERLRRQMSEHFTDPEVVELSFLIG
                     YINMLNLFNNLLDVRYHGDYAILAPHGSPSPG"
     gene            complement(9165..9590)
                     /locus_tag="VGO32_06345"
     CDS             complement(9165..9590)
                     /locus_tag="VGO32_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200404.1"
                     /translation="MPRVEFEANTEDQLVDLVWRWVMGPQRKADEGMDATGGDETELR
                     RRQLMEVLHAIRGVDSRRLVREVAELSLNDEVLVLDEALRQRYGKSTGTAFAGIVAGP
                     NKVMRRVARRDLLVWDAAGGGYRMHPADAEIVLSTWAQP"
BASE COUNT         1623 a         3383 c         3255 g         1539 t
ORIGIN      
        1 cggaagccgg cggccgatca gaacccggcg gctctggcca gggacgtccc ccaggcgcgt
       61 gcccgctcga gctcaccatc cttcagcggg ccgtaggcgc cgtcgacgat gaaccgctcc
      121 gacttgccgg cccgccggta cccggccttc ctcagcttcg cctcgatcgc gccggtcgcg
      181 cccctcggcg accaccagat gcgggtctcg aatgccgcat accagccctc gctgaccggc
      241 agctcgtcga gccaggtgcg gagcagcgga tgggacacgt cgggaggccg cggggccttc
      301 tcaccgccgg cggcgatctg gtcgcgcatg gcatcccgag gcaggccgaa ggcaatcacc
      361 ggcgccccgg cgacgatcag actggcgtcg gcagctgccg cgctcgttgc ctggtcggtg
      421 gtcattgcct ccgcttcggg gccgatgcct gaggcgatgg cccgggcgac ggcctcggtg
      481 ttcccccagt gtgactcgta gacgacgatc gccttcatct cagaactccc tcgttcgcgt
      541 gacttggtcc tctagaaggt aggcggggag cggtcctcga tcacgggcca tccggccctt
      601 tgacacggtc ggtttcggcc ctctaggcca cccaagttcg gccgcgccgg cgcatcacct
      661 cggccagggc ggcgggcgtc agggcgatca cggcgatcgc agcccattcc gtcgccgata
      721 gtggcgacgc gcggaatgcg tcggcgagcg gagggatgta cgggatcgcg gcctgcacgg
      781 cgagccagag gacgcccatt cccgccagca gcaagttggg acgcaggcgc aggagcgagg
      841 acccaagggt ccggttggcg ttcgcccgca cgagctgggc cacgaccagg gtcgtgaacg
      901 ccacccagtt cgcgtgctcc tggtcgcccg agacgctgag gaccagcgcc agcgcggacg
      961 ccacggtgaa ggcaccggcc agacagatcc cgaccagcag gcggcggtcc agtagcggct
     1021 gatcgcgcgg ccgaggccgc accgacatgg cattcggctc ctccgcttct cgctcgaagg
     1081 ccacggccgc cgagatgtcg atgaagaact ccatccacag gatctggatg ggaagcaggg
     1141 ggaggctgat cccgaccacg gtggcgagca ggacgtaccc gagcagcgcg acgtgggtgg
     1201 agaggaggaa tacgagccct ttctggacgt tggcgatcat ccgtcgtccc tcgcggaggg
     1261 catccatcag cgtcgcgaac gagtcgtccc cgagcaccag gtcggccgcc tcccgcgcga
     1321 ccgccgtccc actgcccatg gccaccgcca cgtcggcgcg gtggagtgcc ggcgcgtcgt
     1381 ttacgccgtc gccggtgacc gccaccgtgc gggccgaggc cgcggccgcc tcgaccaggc
     1441 ggagcttgtc ggcgggcgtc gctcgagcca cgatctgcag atccctgatg cgggccgcga
     1501 gggcgtcggg gttcatggcc tggatctcgg caccggtgag gacgatgtcg cccgggagcc
     1561 ccgcttcccg cgcgatcgca cgggccgtgg tcgggtggtc gccggtcacc atcacgacct
     1621 ggatgccggc cgcgagcgcc gtctcgaccg cagcggggat ttccggtcgg aggggatcgg
     1681 cgaacacgag ggtggcggtc ggctgccagt gggcgccctc gctccgcgcg gccagcagca
     1741 gcagtcggcc acctcgatcg gcctcctcca caatcagtcg ctcccagccc ccgaggtctc
     1801 cctgcgccgg tcccagccca ccatcggcgc gcaccagttc gagcaccgcc tcgggcgcgc
     1861 cgagggcgag gtcgcgcagc ccctgctcgt cgtagcaccg ggtcatcgca tacggacgca
     1921 ggtccgtggg cggcgaggcg tcgaggaggg tctcgggatt cagctcgggg acgagatcct
     1981 cgtcggcgag ggcgtcatga atcgcgcgtg cgaaggcgcc ggtccgcccg gttcccgcgg
     2041 cgtgccatgc ctccgcttcc gcccgcagcg cctcgccgag gatccgcgcc cgctcgacgc
     2101 caaccgccgt gcccaccggt gtccgcaccg ccgccaccga cagccggttg agggtcaggg
     2161 tgccggtctt gtcggtgagg atgaagtcca ccgctccgag cgtctcctgc gcgttcaggc
     2221 ggcggacaag gacgtcacgg cggaggaggc ggtaggcgcc aaggccgagg atgaccgcga
     2281 gcaggatggg tggctcctct ggaatcgcgg cgatcgcggc ggccacgccg gcgaggatgt
     2341 tctcgccggc cggattgcct cgcaggaagc ccaggccgac cgtgatcacc accaggccga
     2401 gcgccaccac aagcagaatg cggacgaagc gggccagctc gcgttccagc ggcgagcggg
     2461 gccgggcatg ggccgccagc gagctcgcga tcctgcccac ctcggtcgtc tggccgacgg
     2521 acaccaccac gccctcgccg agcccgccaa ccacgttcgt gccggcgtag gccatcgccc
     2581 ggcgctcgga gagggccgcg tcggacgaat ccgcctcgac cgaggccggc tcgggaagcg
     2641 actcgccggt cagcgtgctg cggtcgacca gcagcgcgtg cgaggtggtg acccgaaggt
     2701 cggcggggat gacatcgcct accgcgagga gcgcgaggtc accgacgacg acttcgcgag
     2761 ccggaatctg gactgcttcg ccgtccctcc gaacccgggc ccgcggcgca ttcgcctggc
     2821 gaagaacctc cagtgcgcgc tcggcgcggt agctggtggc gacgtccgcc gcgacaatgg
     2881 ggacgacccc tatcaggatc aggatgccgt cccggacttc gccgaggatg atggccagca
     2941 cggccgctcc gatcagcatg aggacgaagg gctcgcgcag cgcgtccacg agcgcccgca
     3001 ggatgctcgg accgggcacc gcttccaact cgttcgggcc gttctccgcc agccgcacgg
     3061 ccgcgtcggc gtccgacagg ccggtcgccg ggtcgacatc cagcgacgcg cagacctccc
     3121 tggccgatcg cgcccacggg ctggcagccg cgcgaatcat cggtccgagc atcgcacctg
     3181 gcgatggcga tttcgtgggc cgtctggcac catcgatcgg gagcgatggc acgtatcttc
     3241 gagggcggaa tcgagtgatc gtatggccat gaccgcagtg acccccgacg agaccgtcgg
     3301 tctcaacccg ccactgaccg gcaccgagcc agcgggatgg gacacttgcc tgacatgcac
     3361 gccattgggg cgagttcggg gctgtcttcc accgctggcc gaataaggat ctgaggcagg
     3421 ccatgagagc cgttttgcca cgtcgcgcgg tcgccgagat ccgggacggc cacacgatat
     3481 acatccacgg cggtgcggct acgcccaccg cgcttctcac ggcgctcgcg gaacgaggtc
     3541 cgaagctgaa gggtgtcacg accgttgggc tccacctcga gggaccatgc ccgcacctgg
     3601 cgccggagct ggccgggcac atccggcatc gcgcgctgtt cattggtccc accgcccggg
     3661 atgcggtgaa cgaaggacga gcggactatg tcccgatctt cctatcggac attccgctcc
     3721 tcatcagccg aggcatcctg agcctcgact gcgcgttcat caacgtcagc ccacccgatg
     3781 cgcatggcta ctgttcgctg ggcccgagcg tcgacgcaac tctggcagcc gttcgaagcg
     3841 cgcgaacggt gatcgcccag atcaactcgg caatgcctcg cacgctgggt gacagcttcg
     3901 tccatgtgcg ggaaatcgac ctcatggttg aggtggccca gccaccgcac gagcacccgg
     3961 ccgcggagat cggcgagacc gagcgccaga ttggcgccct cgtcgctgac ctgatcccag
     4021 atggagcgac cctccagctc ggcatcggct ccatccccgc cgcggtgggc gccgcgctcg
     4081 gcgacaagac cgacctgggc atccataccg agctgttcac cgacaccgtc gtggatctcg
     4141 tcgaagccgg cgtggtgacc ggcgcggcaa aagagatcaa caccggcaag atcgtgtcct
     4201 ccttcgtcct cggcacgccg cgcctctatc ggttcatcga caacaacccg atggtcgaga
     4261 tgcggccggt tgactacacg aacgacacgg ccgtcatccg caggttccgg aaaatggtgg
     4321 ccatcaactc cgccatcgag gtcgacctgt cgggccaggt cgtggccgac tctatcggcc
     4381 gccggctcta ctccggggtc ggcggccaga tggacttcat tcgcggcgcg gccctggccg
     4441 aggaggggcg ggccatcatc gccctgcctt ccacggcggc cggtggcgcg gtctcgcgga
     4501 tcaccgccag cctccagccg ggtgccggtg tggtcaccac ccgcgctcat gttcggaccg
     4561 tggtcaccga atggggcgtg gccgagctct tcggacgttc gctcgccgag cgtgcccatg
     4621 ccctcattca gatcgcacac ccggcattcc gagacgagct cttgcgtgcc gcgggccaga
     4681 tgcatctcga ccactgacgt gcggaacgtc cccccgcgag gtgccgaacg gcactcgccg
     4741 cgaccgcggg ctcgagacca ccatcaggca tggctcgtga tgacattacc gtccgctggg
     4801 tgcgcgcaga ggacgcagcc cgcctcgagg cgttctacag cgccctgacc gatgacagcc
     4861 gcgccgcgcg attccacggc gcctgtcgcg gcatcagcca tgagcgggcc gcgcacgatt
     4921 caacggcgga ccaccaccac cgtgacgggt tcgttgccga gtacgagggg cgcatcgttg
     4981 gccacctcgt gctcgagccc atgcgtgccg gtgcggagga ggtcgcggtg gccgtcgcgg
     5041 acgatcttca gcatcacggt gtgggatcac tgctcatggc ggccgcggtt gcgtccgctc
     5101 ggctgcgtgg tgtgcggcat ctgcaggcct ggatccgacc cgacaacgaa gccatgcggc
     5161 gtttgttcac cggcacctcg cggcccaccc acctcatcgc ctgggacggc tacgacgccc
     5221 gatacgagct tgacgtgccg cccttcccgc acgcggcctg attcccgcct gtcacgcgct
     5281 ggcggagtct cgaacgacgc tcacgaggca acagcctcga ccagcgcccg cagcggcgcc
     5341 tcgaatcgag cgatgtctcc gaaggtgtgg atcgactccg cctcctcgtc ggtcatcggc
     5401 ccaacgacga tcttcgacgc cttgagcacc gtctcctccc cgtcggcctc cagctcgtac
     5461 tcgctgtcga ggatcgtgcc gggcatgacc cggccgcggg tggcaaaccg attggggcgg
     5521 tcgtacacgg tgacgtgccc gtagaggtat cccatcccgt ccccccagtc ctcgtagctt
     5581 gccccgccga cccgcggctc gttgagcacg gcccgggtcc gtgtgtcccc gtaggagtgg
     5641 gggaaccact ccagggtctc gtcgaacacg gcgcggaaga cgcgctcggg ggtggcccgg
     5701 aagcggagct ccgtttcgat tcggacgacg cggacgtcgt cgaccagcac ggccatggat
     5761 ggacctcctt ctgacgatgc gagatggcgc tcgagggcaa gcatttcggc ggcagagcgg
     5821 tcggcgaaag ggacgaccca tcgctcgtac cagcgccgca gcggcaccgg gttgaggtgg
     5881 ttgaaccgtt gccggccccg tcgccgaacg acgaccaggc ccgcctcggt caggacgccg
     5941 aggtgctgca tgaccgcgaa gcgggtcacg gtggggatgg ctccggccag ttcgccggtc
     6001 gtcttcggcc cgtcgcctag caggtcgagc agctgtcgcc gaagtgggtt cgccagggca
     6061 cgccagacgt cctgctccgg tcgctcgccg gattcgatgc tgatcgagct caacatgtga
     6121 taagactatc acataagatg ggctcacagg gaaccgggag gggactcccc gaatgggcta
     6181 ggcggcgagt gcgatcggcg cggacggcta acgctccggt ctgcccgctt tgcggcgcga
     6241 cccaatcgcg gacacgtctt cagcaggatc ctccagaagg agtacgttta gccgtgcacg
     6301 gagggtacta tcgattgacc agtccaatca ccgccactct cgccgcccac cttgctgatc
     6361 tggccgcgtt cggattggcc atcagcgtgt atggcatagc gggcgagagc aacccaatcg
     6421 cgtatcagct gttcatcgtc ggtggcatac cgctgctcgt ggcggtgaag gtggcagggt
     6481 cactggttgc cgccctgatc gtctcccgcc gccgccggtg gctccccctg gcggcaggca
     6541 gcggtctgct tggcgcggta accgcgctga tggtgctgct ctagcgcgac gctcactcga
     6601 tcgcgttcag ccgcccgtcc tgatacgttc cgggctcgct cgagcctggc acgaaaccgg
     6661 ggcggctagc tcagctggtt agagcacttg cttgacatgc cgtcggccct ggtgcgccgt
     6721 aggtcggggc actttgagcg cgaatagcac atgcgtaaga ccccgagtta tcgtggtatc
     6781 caggcgcggg gctcccgaca cccagctcgg ttccctagcc tccacggtcg aatcgccggg
     6841 agtccggctg ccacgaacct agcgcttgcg ccgccgaagt cggaccaacg gtaggaggag
     6901 cccgacaatg gtcgtgatca ggtacaggcc aaccgcggca attggaagga cgatcgccac
     6961 ggcgatggcc accacgttca gcacgatgat gctccagcgg cggcgaacct ttgcgtcgag
     7021 ctcgtcgtcg gccacgctgt ccaggacgag tgaccgctcg ctcgcgatgt aaaacatcag
     7081 cagactcagc gtggacgacg ccacgagcag gttcgcgccg tagatcacga ccgcagctgt
     7141 cacgtccgga ccctccagat gggtgaccat cagctcggtc gagaatggca gcaggccgac
     7201 gaagagcagc atgagcaagt cgatgccata ggcgatagcg tctgcctctc gcatcagctt
     7261 cgtcatcccc gcgtgagcga cccagatccc accgatgaac acgaagctga tcaggtagcc
     7321 aaggaactcg tgccattgct cggccagggt cggcagcaac ggagcctcaa gagccgggac
     7381 cgtgagctcg aggacgagca gtgtgatggc aatggcgaag acgccatccg taaacgcgtt
     7441 gaagcgatcg agcggaagga attcggaggc tgggcgcgtg cggccatcac tctccggtgc
     7501 cggcgtgtcg gtcagcgcct cgtcagcgga ctggtccgag tccatcggat cacatggtag
     7561 aggccgtgcc ggcagccggc tcgcagcgcc accgggcaac cgcgggggcg gcgcatactg
     7621 gccgtccgtc ccagcgtagt agccagccgg ctccaatacc ggcatcccgg aggaattcat
     7681 gcgactaacc ccaccgcgaa tgaccgcagc catcctcgtc cttgctctag ggctggtcgc
     7741 ggcatgcacg cccgcgtccg accagccgag tgcagagcag gcgctgtgcg atgccctggc
     7801 caccttccgc accagcgtcc gggcgatcgc cgacctgtct ccgacgacag cctcgatcga
     7861 ggatctgcag gctggcgttg attcggcgca ggaggcctgg ggtggcgtcc aggatgccgc
     7921 cgaagccctc ggcgaagctg acacgtccgc ccttgagacg gcttggaccg accttgcgga
     7981 tcagatcagc aacttctcca ccgacatccc gctgcgcgaa gcgatcggcg agatcacagc
     8041 cggcgtcgat gaggtgcagg ctacatacga cgagatccga aacggcttgg gctgcgaagg
     8101 cagctgaccc ccccacatcg cttggcactg cactgggccg acaccgatgc ccacctcggc
     8161 tccctaggct ccccgagagg tcgaatcgcc gggagtccgg cggtatgacc ctaggggtgg
     8221 cggcctactg gcggcttagc accgcgagcg ttcgccgcga ccagggtgcc gaggggggga
     8281 ttggcggggg ctgatattct ctgccgcgtt ggaggtctgg ctgtcagcgc aatacacctt
     8341 caggcgatga catgggaacg ctccctagta tgcggtggtg taggtcaagg ctccaacgcg
     8401 ataactacca gtcatgtgct cttcccgagc acgaaacgat gggcggctag ctcagctggt
     8461 tagagcactt gcttgacatg caagaggtca ctggttcgag tccagtgtcg cccaccaccc
     8521 aacccgatcg cgttgaccgg ttttggcctg ccaccgagga gtagccgatg cgcgtcgccg
     8581 tcagtccgga gcagatcagg gcggcattcg aggccgtgaa ggacgatcct tcgttcgacg
     8641 agagccgggg gctctgggag cgtggccggc caccggtcga gatgatccag gccatgtgcc
     8701 tgcgccccga gatcctgcgc gggttcgcgg gcttcggaaa cgccgtctac cccggcggcc
     8761 tgctcgagcg acgggtcaag gagctggtga tcatcaccgc ctcgcagacc aacgagtgcc
     8821 agttctgcac cgcctcgcac gtggacctgg tgggaatcgc ggacatcatc caggagcccc
     8881 tcgtcgtgat cgacgacacc gaatcactcg cgccccgcga acgcctcgcc gtcgaataca
     8941 cgcgagccgc gatggccgac tcgaatgcca ttcccgaacg gctgcggcgg cagatgagtg
     9001 agcacttcac tgatccggag gtcgtcgagc tctcgttcct gatcggctac atcaacatgc
     9061 tgaacctgtt caacaacctg ctggacgtcc gctaccacgg cgactacgcg atcctggccc
     9121 cgcacgggtc cccatcgccc ggttgaggcc gatgggtcac cgccttatgg ctgcgcccag
     9181 gtactgagca cgatttcggc atcagccggg tgcatccggt agccgccgcc ggcagcgtcc
     9241 cagacgagga gatcccgccg cgcgacgcgc cgcatgacct tgttcgggcc ggccacgatc
     9301 ccggcgaacg ccgtgcccgt gctcttgccg tatcgctgcc gcagcgcttc gtcaaggacg
     9361 aggacctcgt cattcaatga gagctccgcg acctcgcgca ccaggcgacg gctgtcgacg
     9421 cctcggatcg cgtggagcac ctccatcagc tggcggcggc gcagttccgt ttcgtccccg
     9481 ccggtcgcgt ccatgccctc gtccgccttg cgttgggggc ccatcaccca tcgccagacg
     9541 agatcgacga gctgatcctc ggtgttcgcc tcgaattcga ccctcggcac ttgtaactcc
     9601 ttgaaagact agtagcagat tagacgcgag cctatcacag gtcaatctgt ggtagactgc
     9661 cgtcaacggc tgtaaaagct gcgaaccgtc tgatctcctg gccttcgaaa gaagtcggtc
     9721 acgagcgatt ccgcttcgta tcgaccgccg aaccacagag gagaacgacg tgaagcacat
     9781 tcgaatcgcc acgtacgacc
//
LOCUS       DASYGU010000227         6990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1372916, whole genome shotgun sequence.
ACCESSION   DASYGU010000227 DASYGU010000000
VERSION     DASYGU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372916"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGO32_06350"
     CDS             <1..192
                     /locus_tag="VGO32_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEV7200405.1"
                     /translation="MAATISEPVETIEDVVEPYLIQAGLLARTPRGRQLTPSAWTHLG
                     LTPPIHATVEATPQPGLFD"
     gene            353..1678
                     /locus_tag="VGO32_06355"
     CDS             353..1678
                     /locus_tag="VGO32_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV7200406.1"
                     /translation="MEALASFFKFHERGTTFGTEVRAGITTFMVMVYIVAVNPLILSA
                     AGIDAAAAAAATALVAAVMTILMGIVANVPLALAAGLGINGIVAFGLILGAGLTPAGA
                     MGVIVLEGLAITVLVLAGLREAVMDAVPMALKRAIAVGIGLFILFIGFVDGGLIVGQE
                     GPVPVAFVFPNSPAAWVTLSGLAITIILYARKVPAALIISIVLTSVVAYLLGVSTLPD
                     SFSATPNFSTLGQFDFSVFDSLPLAVALLTIFTIMLADFFDTMGTATAITEQAGLMTE
                     DGKVPQIRRLLLVDSLAAVAGGAAGISSNTSYIESAAGVSEGGRTGFSSIVVGLLFAV
                     MILFSPLVALVPLPATAAVLILVGFFMVSTLVKDIDFTNFEDGFPALLAMILMPLTFN
                     ITVGIGAAFIVWVFIKVILGKFREVHWLMWIVSLAFLIYFLQEVVKAYI"
     gene            1762..2832
                     /gene="queA"
                     /locus_tag="VGO32_06360"
     CDS             1762..2832
                     /gene="queA"
                     /locus_tag="VGO32_06360"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV7200407.1"
                     /translation="MTDPERRLRVDDFDYDLPTEAVAQTPAEPRDASRLLVLDRPDSR
                     PRRPALTEARFADIGTWLEPGDLLVVNDSRVLRSRLRGRRAGGGAAEVLLLRPMPDGR
                     WEALVRPGRRLPPGGSVQLSSGDAVIVGESVGEGIRAVALPRDPIALMADAGEMPLPP
                     YITAREAPDERYQTVYAEPPGSAAAPTAGLHFTPRLLDELAAAGVERASVTLHVGLDT
                     FQPLDGTFVDEHRIHREWYVVPEATRSAIVRTKAAGGRVVAVGTTTTRVLETLARTGA
                     SKGWTDLYIIPPHAYRSVDALITNFHLPRSSLLLLVTAFVQDGMPDVGPLDARDALLA
                     AYQAALASGFRFYSFGDAMLIR"
     gene            2856..4028
                     /gene="tgt"
                     /locus_tag="VGO32_06365"
     CDS             2856..4028
                     /gene="tgt"
                     /locus_tag="VGO32_06365"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV7200408.1"
                     /translation="MPPPITFEASPTKIGRARLGQLETPHGGVDTPQFMPVGTQATVK
                     ALTPADLRSAGTTILLGNTYHLSLRPGEDRIARLGGLHAFMAWDGPILTDSGGYQVFS
                     LGHLRTVDDDGVTFASHLDGSVVRLTPERAIAIQEALGSDIAMVLDHLVGADAPVEAV
                     ADAMARTHAWAARSLHARQRPDQAVFGIVQGGIDQPLRRTSAEAIAALPFDGIAIGGL
                     SVGESKAAMASALEAVAETLAGDARPRYLMGVGSPEDFFVAIEHGVDLFDCVLPTRLA
                     RHGQLWTSEGRLRLGNSAVQDDPRPVDPACRCETCRDYSRAYLAHLFRAGELLVHRLT
                     SVHNLTYTLDLMRAIRQALTDGGYEALRDRVTGQFAAPDDDPQSASNDPIQDVVRD"
     gene            4066..4215
                     /locus_tag="VGO32_06370"
     CDS             4066..4215
                     /locus_tag="VGO32_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200409.1"
                     /translation="MGNRDRPKKQPKRQKKDKAHKAAPPLVYEPTTVEVIRPTRKPRD
                     EPDAG"
     gene            4260..5372
                     /gene="recA"
                     /locus_tag="VGO32_06375"
     CDS             4260..5372
                     /gene="recA"
                     /locus_tag="VGO32_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV7200410.1"
                     /translation="MDATAAAPKTQANGEKTPANGEKTPANGERARAVDAAILQIEKQ
                     FGQGAIMRLGDTGARTAVDVIPTGSVALDLALGVGGVPRGRITEIFGPESSGKTTLCY
                     HIAANAQKKGGIVAFIDAEHALDPGYARNLGVNVDELLVSQPDTGEQALEIAETLIRS
                     SGVDVVIVDSVAALVPRAEIEGEMGDSFVGLQARLMSQALRKLTGAVSRSNTALVFTN
                     QLREKIGVMFGNPETTPGGRALKFYASIRMDIRRIETLKNGTDAFGSRTRVKVVKNKV
                     ASPFRIAEFDILYNEGISREGGLLDVGIASGVLTKTGAWFNYGDTRLGNGREASREFL
                     KANPGMADAIEEEIHGRLASITVDVTAAESESATPA"
     gene            5377..5898
                     /locus_tag="VGO32_06380"
     CDS             5377..5898
                     /locus_tag="VGO32_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEV7200411.1"
                     /translation="MTTPRTRSRTPREPKPPPDAEQAFEIAGRFLGTRPRSRWEVEQR
                     LRRSGTTDEVVAVTLERLERIGFVDDTAFARWWVEQRDRHSPRGRMALETELRAKRIP
                     PAVIAALRDVPPDEEPVSEDDRADAALERHLKGRPLPDDPKALQRLGMYLVRRGFDPA
                     TARAALRRRGAGV"
     gene            5915..6868
                     /locus_tag="VGO32_06385"
     CDS             5915..6868
                     /locus_tag="VGO32_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7200412.1"
                     /translation="MRFALMTEPQQGLSYGEILAAVRAAEAAGLEAFFRSDHYRSFPG
                     PADNPTTDAWATLAGLARETSTIHLGTLVSPFTYRVPGNLAKVVATVDEMSGGRVEVG
                     IGSGWFAEEHARYGIPYREPISVRYDQFEEALTVIHGLWTEPDGWSFTGQDWQVTDAL
                     FRPRPTFSGKRHPHLILGGDGLPRQAALVARFADEFNRSSATPDRLRRSYERVREACV
                     AIGRDPEEVVRSAMIGVLVAETEAELGERVHAQLRMLGSDEGADAWLADRRGRWIMGT
                     PEQAHEQIGELEAAGAQRIMLQDFLPRDLGMIGLLGQIAAG"
BASE COUNT         1058 a         2457 c         2392 g         1083 t
ORIGIN      
        1 atggcggcca cgatcagcga gccggtggag acgatcgagg acgtggtcga gccgtatctc
       61 atccaggccg ggctgcttgc ccgcacccca cgcggccggc agctgacccc gtccgcctgg
      121 acccacctgg gactgacgcc gcccattcac gcgacggtcg aggccacccc ccaaccgggc
      181 ctgttcgact gattttcggg cccgttccgc acgatctggt ccggtccggg ggagtcgcgc
      241 agaaaacggc cgatatccac attcggtccc gctacgatcc ggctccgggt cgcgtcacgc
      301 acccagccct tggtgcggcg gcgatccagg gagcgaggag ggccccctca gcgtggaagc
      361 attagcttcg ttcttcaagt tccacgaacg gggtacgacg ttcggcaccg aggttcgggc
      421 cggcatcacc acgttcatgg tcatggtcta catcgtggcc gtgaacccac tcatcctgag
      481 cgcggcgggt atcgatgccg cagcggccgc cgccgcgaca gccttggtgg ctgcggtcat
      541 gacgatcctg atggggatcg tggccaacgt tcctctcgcc ctggccgccg gcctgggcat
      601 caacggtatc gtcgcctttg gtctcatcct gggagccggt ctcacgccgg cgggcgccat
      661 gggcgtcatc gtcctggaag gcctggcgat cacggttctc gtcttggcgg ggcttcgcga
      721 agccgtcatg gatgccgtcc cgatggccct caagcgggcg atcgcggtgg gcatcggcct
      781 gttcatcctg ttcatcggct tcgtcgatgg cgggctgatc gtcggccagg aaggtccggt
      841 cccggtcgcg ttcgtgttcc ccaactcgcc cgcggcgtgg gtaaccctgt ccgggctcgc
      901 gatcacgatc atcctctacg cccggaaggt gccggccgcg ctgatcatca gcatcgtgct
      961 gaccagcgtg gttgcctacc tgctcggcgt gagcacgctg ccggattcgt tctcggcaac
     1021 cccgaatttc tccacgctcg gacagttcga cttcagcgtc ttcgacagcc tcccgctggc
     1081 ggtcgcgctg ctgacgatct tcacgatcat gctggctgac ttcttcgaca cgatggggac
     1141 ggccacggcg atcaccgagc aggctggtct catgacggag gacgggaagg tcccgcagat
     1201 ccgccggctg ctcctcgtcg actccctggc cgcggtcgcc ggtggggcgg cgggaatcag
     1261 ctcgaacacc agctacatcg aaagcgcagc cggggtgtcc gaaggcggcc gcaccggttt
     1321 cagctcgatc gtggtcggcc tcctcttcgc cgtcatgatc ctgttctcac cgctggtggc
     1381 cctcgtgccg ctgccggcaa cggccgcggt cctcatcctg gtcgggttct tcatggtttc
     1441 gaccctggtc aaggacatcg acttcacgaa cttcgaggat ggcttcccgg cgttgctggc
     1501 gatgatcctc atgcccctga ccttcaacat cacggtgggc ataggcgccg ccttcatcgt
     1561 ctgggtgttc atcaaggtca tcctgggcaa gttccgggag gtccactggc tgatgtggat
     1621 cgtctcgctc gcgttcctga tctacttcct gcaggaagtc gtcaaggcct acatctaggc
     1681 cactccacac cgacgacgcc cggccgggcc atcccgccgg gcgtcgtgct gctccgtcgc
     1741 ggttcgggct agccttccgc cgtgaccgat cccgagcggc gactccgcgt cgacgacttc
     1801 gattacgacc tgcccaccga ggcggtagcc cagacgccgg ccgagccgcg cgacgccagc
     1861 cgtctgctcg ttcttgaccg cccggattcg cggccacggc ggcccgctct gaccgaggct
     1921 cgcttcgccg atatcgggac atggctggag ccgggcgacc tgcttgtcgt caacgacagc
     1981 cgggtcctcc gctcccggct ccgcggacgg cgcgccggcg ggggagcggc ggaggtcctg
     2041 ctccttcggc cgatgcccga tggccgctgg gaagcgctcg ttcgtcccgg ccgtcgtctc
     2101 ccgccgggcg gatcggtcca gctgtcgagc ggcgacgcgg tgatcgtggg cgaatcggtg
     2161 ggggagggca tccgagcggt ggccttgccc cgcgacccga ttgccctgat ggccgacgcg
     2221 ggcgagatgc cgctcccgcc gtacatcacc gcccgcgagg ctcccgacga gcggtaccag
     2281 accgtgtacg ccgagccgcc gggctcggcg gccgccccaa cggccggcct ccacttcacc
     2341 ccgcgcctgt tggacgagct ggcggccgcg ggggtggagc gggcgagcgt cacgcttcac
     2401 gtcgggctgg acacgttcca gccgctggac ggcacgttcg tggacgagca ccgcatccat
     2461 cgcgagtggt atgtcgtgcc ggaggcgacc cgctccgcga tcgtgcggac gaaggcggcc
     2521 ggtgggcggg tggtggcggt gggcacgacc acgacccggg tgctcgaaac cctggcccgg
     2581 accggggcct cgaagggctg gaccgacctg tacatcattc cgcctcacgc ctatcgatcg
     2641 gtcgatgcgc tgatcacgaa cttccacttg ccgcggagtt cgctcctgtt gctggtgacc
     2701 gccttcgtcc aggacgggat gccggacgtc gggccactgg acgcgcgcga tgcgctgctg
     2761 gcggcatacc aagccgcgct ggccagcggc ttccgcttct actcgttcgg cgacgcaatg
     2821 ctcatccgct gagcccgcgt cggtatcctc gggtcgtgcc accgcccatc acgttcgagg
     2881 cctcgccgac gaaaatcggt cgcgccaggc tggggcagct cgagacccca catggcgggg
     2941 ttgacacgcc ccagttcatg ccagttggca cgcaggcgac ggtcaaggcg ctgactcccg
     3001 cggacctgcg gagcgccggg accacgatcc tgctgggcaa cacctatcac ctgtcgctgc
     3061 ggccgggcga ggaccgaatc gcgcggttgg gcggactgca cgccttcatg gcctgggacg
     3121 gacccatcct gaccgactcg ggcggctacc aggtcttcag cctcggccat ctgcggacgg
     3181 tggacgacga cggcgtgacg ttcgcctccc acctcgatgg atcggtcgtc cggctcacgc
     3241 ccgagcgggc gatcgcgatc caggaggcgc tcggaagcga catcgcaatg gtcctcgacc
     3301 acctggtggg ggccgacgca cccgtggagg cggtcgccga tgcgatggcc cgcacccacg
     3361 cctgggcggc gcgttcgctc cacgcccggc agcggccgga ccaggcggtc ttcgggatcg
     3421 tgcagggcgg catcgaccag cccctgcgcc ggacatcggc cgaagcgatc gccgccctgc
     3481 cgttcgacgg catcgccatc ggcggcctgt cggtggggga gtcgaaggcg gccatggcgt
     3541 ccgcgctcga ggcggtcgcc gagaccctgg ccggtgacgc ccgtcccaga tacctcatgg
     3601 gtgtgggctc acccgaggat ttcttcgtcg ccatcgagca cggggtggac ctgttcgact
     3661 gtgtcctgcc cacccgcctg gcccgtcacg ggcagctgtg gacctccgag ggacgcctgc
     3721 gcttgggcaa ctcagcagtc caggacgatc cgcggccggt ggatcccgcc tgccgctgcg
     3781 agacatgccg tgactactcg cgggcctacc tcgcccacct gttccgggcg ggtgagctgc
     3841 tcgtccatcg gctcacttcg gtccataacc tcacctatac tctcgacctg atgcgggcca
     3901 tccgccaggc gctcacggat ggcggctacg aggcccttcg cgaccgcgtg acaggccagt
     3961 tcgcggctcc ggacgacgac ccgcaatcag ccagtaacga cccgatacag gacgtcgtcc
     4021 gagactgatt ctgcgtcgga caccacaggg acgagtgata cgccgatggg caatcgagac
     4081 cgccccaaga agcagccgaa gcgccagaag aaggacaagg cccataaggc cgccccgccg
     4141 ttggtctacg agcccacgac ggtggaggtc atcaggccga cccgcaagcc gcgcgacgaa
     4201 cccgacgccg ggtgacccgc gaagccaccc gagcagtcag gaaggagccc ggatcccgca
     4261 tggacgcgac agcggccgcc cccaagaccc aggccaacgg ggagaagacc ccggccaacg
     4321 gagagaagac cccggccaac ggcgaacgcg cgcgcgcggt cgatgcggcc atcctccaga
     4381 tcgagaagca gttcgggcag ggagcgatca tgcggctggg tgacaccggc gcccggaccg
     4441 cggtcgacgt catcccgacc ggttcggtgg cgctcgacct cgcgctcggc gtgggcggcg
     4501 tgccgcgcgg ccggatcacg gagatcttcg gccccgaatc gagcggcaag acgacgcttt
     4561 gctaccacat cgcggccaac gcccagaaga agggcgggat cgtcgccttc atcgatgccg
     4621 agcatgcgct ggatcccggc tacgcccgca acctgggcgt gaacgtggac gagctcctcg
     4681 tctcgcagcc ggataccggt gagcaggccc tggagattgc cgagaccctg atccgaagca
     4741 gcggcgtgga cgtcgtcatc gtcgactcgg tcgccgcgct cgtgccgcgg gcggagatcg
     4801 agggcgagat gggggactcg ttcgttggtc tccaggcccg gctgatgagc caggccctcc
     4861 gcaagctgac cggcgcggtg agccgttcga acaccgccct cgtgtttacc aaccagctgc
     4921 gcgagaagat cggggtcatg ttcggcaacc cggagaccac tcccggtggc cgcgccctga
     4981 agttctacgc ctcgatccgg atggacatcc gccggatcga gaccctgaag aacgggaccg
     5041 atgcgttcgg cagccggacc cgggtcaagg tcgtgaagaa caaggtcgcc tcgccgttcc
     5101 ggatcgccga gttcgacatc ctctacaacg aggggatcag ccgcgagggc ggcctgctcg
     5161 acgtgggcat cgcctccggc gtgctgacca agaccggcgc gtggttcaac tacggcgaca
     5221 cccggctcgg gaacgggcgc gaggcctcgc gggagttcct gaaggccaac ccgggcatgg
     5281 ccgatgcgat cgaggaggag atccacggtc ggttggcgtc gatcacggtc gacgtgacgg
     5341 cggccgagtc ggagtccgcg accccggcct gaccgggtga ccacaccgcg aacccggtcc
     5401 cgaacgccca gggagcccaa gccgccgccc gacgccgagc aggcgttcga gatcgccggc
     5461 cgctttctcg gcacgcggcc acgctcccgg tgggaggttg agcagcgcct ccgccgatcg
     5521 ggcacgaccg acgaggtggt tgccgtcacc ctcgagcgcc tcgagcggat tggcttcgtg
     5581 gacgacaccg cgttcgcccg gtggtgggtc gagcagcgtg atcggcattc gccgcgggga
     5641 cggatggcgc tcgagaccga gctccgcgcc aagcgcatcc cgccggccgt catcgccgcg
     5701 ctccgtgacg tgccgccgga cgaggagccg gtcagcgagg acgatcgggc cgatgcggcc
     5761 ctcgaacggc acctgaaggg acggccgctg ccggacgacc cgaaggcgct ccagcggctg
     5821 ggcatgtacc tcgtccgccg cgggttcgac ccggccaccg cccgggctgc gctccgccga
     5881 cgcggcgccg gggtctgagt cgataatcgc gcccatgcga ttcgcgctca tgaccgagcc
     5941 ccagcagggg ctgtcgtacg gcgagatcct ggccgccgtc cgcgccgcgg aggccgccgg
     6001 gctcgaagcg ttcttccgat ccgaccatta ccgcagcttc cccgggccgg cggacaaccc
     6061 gacgaccgat gcctgggcca ctctggccgg gcttgcccgc gagacatcga ccatccatct
     6121 cgggaccctc gtctcgccgt tcacgtaccg cgtgccgggc aacctggcca aggtcgtggc
     6181 caccgtcgac gagatgagcg gcggccgggt cgaggtcggc atcggttcgg gctggttcgc
     6241 cgaggaacat gcccgctacg gcatcccgta tcgcgagccg atctcggtcc gctacgacca
     6301 gttcgaggag gccctgaccg tcatccacgg gctgtggacc gagcccgatg gctggagctt
     6361 cacgggacag gactggcagg tgaccgacgc gctgttccgg ccccgcccca ccttctcggg
     6421 aaagcgccat ccgcacctca tcctgggcgg cgacggcctg ccccgccagg cggcgctcgt
     6481 ggcccgcttc gcggacgagt tcaaccgcag ctcggccacg ccggatcggc tgcgacgcag
     6541 ctacgagcgc gttcgcgagg cgtgcgtggc catcggtcgc gacccggagg aggtcgtgcg
     6601 ctcggcgatg atcggagtgc tcgtggcgga gaccgaagcc gagctcggcg aacgggtcca
     6661 cgcccagctg cggatgctgg gaagcgacga aggggccgac gcgtggctgg cggaccggcg
     6721 cggccgctgg atcatgggca cgcccgagca ggctcacgag cagatcggcg aactggaggc
     6781 ggccggcgcg cagcggatca tgctccagga cttcctgccc cgcgacctgg gcatgatcgg
     6841 gctcctgggc caaatcgcgg ccggttgagc gcgcgcgggg acccgcccgg gcagttggag
     6901 tagagtaccc cgcaccgatc gacggaacgc tgccgtgatc ggtttgagta cgacattttg
     6961 cgggcccgac cgggcacgcc gggaccgaaa
//
LOCUS       DASYGU010000228         7440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1475742, whole genome shotgun sequence.
ACCESSION   DASYGU010000228 DASYGU010000000
VERSION     DASYGU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475742"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VGO32_06390"
     CDS             complement(<1..234)
                     /locus_tag="VGO32_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200413.1"
                     /translation="MTRTSRAADPDPASLRALEFAAITERLAARTAFAPSHELAEGLL
                     PVGDATHVALLQDQTDEAARLVELHPDATIGGAR"
     gene            complement(209..796)
                     /locus_tag="VGO32_06395"
     CDS             complement(209..796)
                     /locus_tag="VGO32_06395"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEV7200414.1"
                     /translation="MTSPADRRWYDRSALEVAPELLGAVVIHDSADGRVAVRVVETEA
                     YLGPEDLAAHSARGQTARNEPMWGPPGHAYVYRVYGIHDCLNVVCGPGRKPEAVLLRA
                     GEVVEGVTLAQARRGERVPIRRLASGPGNLGRALGVDRNLSGSDLLGGPISIRVGQLP
                     ALIDTRSRIGVDYAGAWAARPYRFVIADDPHVSRG"
     gene            complement(793..1362)
                     /locus_tag="VGO32_06400"
     CDS             complement(793..1362)
                     /locus_tag="VGO32_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV7200415.1"
                     /translation="MEFIAQFTIADLVVLLTLAGGVLVGFQQGLLRYILNCIVVLVAF
                     VLASVLKGPIADSLSGVWHLGTPDEQELWIYITLMAVGVIGGFLLVRTLYGKQRLPLV
                     RQVDEVLGAVMGVLFVALIYTFSWVVLASFFQLAGEASVESAGILGSIYDILNDSFIL
                     GWFREWLLPIAGFVVRPFVPEEIAAFLTF"
     gene            complement(1429..3870)
                     /gene="pheT"
                     /locus_tag="VGO32_06405"
     CDS             complement(1429..3870)
                     /gene="pheT"
                     /locus_tag="VGO32_06405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV7200416.1"
                     /translation="MRVPLTWLAEYVDLELAPAQLAERLTLLGFEVKTVEPAGGEWSG
                     VIVGRVLEVDRHPNADTLWLTNVDVGASDPLEIVCGAQNLVVGQLVPVAVPGSTLPGG
                     RRIERTKIRGAVSNGMLCSPIELGLGDDGDGILILGTADEHEVGTELAAAVGDTVLDV
                     DVKPNRGDALSMIGLAREIAAATGAEVRWPAMTVTEADPPTADAVSVKIEDAALCPRF
                     TARLLDGVGPGVTPDWMAQRLVAAGMRPVSPVVDVTNYVMHEMGQPMHAYDADRIPGG
                     RIVVRRARSGESLETLDHLDRTLDERMLVIADEDRPIGLAGIMGGADTEVTDATRRVI
                     LESAIFHGPTVRNAARRLALRSEASMRHEKGISMDLPRMAADRAASLIAAITGARIAT
                     GIVDNDPGPHAPRVVELDTARTERLLGFGVDGAEATRWLTPLGFTVADEGERRVAVTV
                     PLYRNDVLAAADVAEEIARARGYDRIEGTRSPAEPPLSRPEPSARRHALRRILAGLGL
                     DEVLTHALIGPDDLRRSGFDPDAAHLVRVANPLSGEHAILRPVPYPSVLAVLAENVRQ
                     RRNDLAIFEVGKTYHYKPTGGGDELAQAGGGYREAWTVGIGLLGNATGRQPGEVPRPW
                     DVADLQGILDAVHEALGLPAPTYRPESPEERHAHLHPGRAARMVDGAGRSYGSVGEVH
                     PTVADAWGLPSRPLVAAIHVDPHGLFDLPGRSERAAPVPAAQPVDRDLAVVLDAATPV
                     GELLRLTRQNGGPLLADVTLFDVYRGGQIGDGRVSYGLSLRFQPLSAADERAVERAMD
                     KISGALRHHLAAEIR"
     gene            complement(3873..4973)
                     /gene="pheS"
                     /locus_tag="VGO32_06410"
     CDS             complement(3873..4973)
                     /gene="pheS"
                     /locus_tag="VGO32_06410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV7200417.1"
                     /translation="MSTPTDAAALLAEVARVRTDAEARLAAAADAADVEAIRVAVLGR
                     SGSLTAALRGLSALPDADRPAAGAAANEARVALEAAITERGRALAAGELDLQLAGEAL
                     DMTAPGRPARLGHLHPLMTVERDLREIFHGFGFEVFEGPEIENDLMNFELLNIPPDHP
                     SRDLWDTLYVAEPGSVEAGEPRPAADGTILRTHTSPVQIRAMRELTPPIRVLMPGRCY
                     RYEAVDAAHGFEFFQVEGLVVDRTTSLADLKGMLEEFARAMFGDGTTTRFRPGYYPFT
                     EPSAAFDIGCLVCGGPGCPACGRSGWMTILGAGMVHPVVLRNGGLDPDEFQGYAFGMG
                     LDRLTLLRHGIPDLRLLLSADLRFLEQFSGGR"
     gene            complement(5075..5434)
                     /gene="rplT"
                     /locus_tag="VGO32_06415"
     CDS             complement(5075..5434)
                     /gene="rplT"
                     /locus_tag="VGO32_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV7200418.1"
                     /translation="MPRVKRGVPAHRRHKRLLKQAEGRRMTRSKLIRPAREALFHAMR
                     YAYRDRRDRKRDMRRLWIQRINAASRSHGMPYGRFMAGLKSAGIEVNRKQLADLAVRE
                     PTAFGHLVEMSKATFTS"
     gene            complement(5444..5641)
                     /gene="rpmI"
                     /locus_tag="VGO32_06420"
     CDS             complement(5444..5641)
                     /gene="rpmI"
                     /locus_tag="VGO32_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV7200419.1"
                     /translation="MPKMKTHKGTAKRYRKSGSGKWLRPKAWRGHHLQIKSSRRKRRY
                     AGMAQVSSTHAAQLKRLMPYE"
     gene            complement(5663..6313)
                     /gene="infC"
                     /locus_tag="VGO32_06425"
     CDS             complement(5663..6313)
                     /gene="infC"
                     /locus_tag="VGO32_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV7200420.1"
                     /translation="MTRELRVNDMIRIKEVRVIDDEGQQLGVMQTADALALAQERGLD
                     LVEVAPTAVPPVCKILDYGQYKYEIQKRERETKKKQKSQTFKEIRFRVKIDTHDLATK
                     TRRASEFLDDGDRVKVTVQFRGRELNHPQLGRNLLDKAAEMIGDHGVIERAPLMEGRS
                     LFIVMAPPTAKADRHDDQPDLTDGEAAAEGETIGEALQAKGQVPEVAEEPPAATPA"
     gene            complement(6427..>7440)
                     /locus_tag="VGO32_06430"
     CDS             complement(6427..>7440)
                     /locus_tag="VGO32_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEV7200421.1"
                     /translation="AVPSGLPQFAVPAFDLVEVGNIAVAALAISLVAFADTSVLSRSY
                     ATRLGDRVDQDQELFALGAVNVAAGLFQGFPISSSSSRTPAAEAAGARTQLTGVTAAL
                     AIGIVLVFATGVLAPLPSATLAGIVMVAVIGLIDLAVLRRLFAINRADFGLAIAALLG
                     VALVGVIAGIGIAVGLSAATLLWRAWHPHTAVLGRVDGLKGYHDRERHPEGRQIPGLV
                     LFRFDAPLFFANADRFREAVLDAIEAAEPPIRRVVVAAEPMTDVDSTAEDMLSELIDQ
                     LNRVGIGLSFAELKGPVKDKVVRYGLEAQLAQPPFPRTMGEAVHTYLEEHHVPWSDWE
                     DAD"
BASE COUNT         1126 a         2619 c         2593 g         1102 t
ORIGIN      
        1 gcgcgcgccg ccgatggtgg cgtccggatg cagctcgacg aggcgcgccg cctcatcggt
       61 ctggtcctgg agcagcgcga catgggtcgc gtcacccacc gggagcaggc cctcggccag
      121 ctcatgcgac ggcgcgaacg cggtgcgcgc ggcgaggcgc tcggtgatcg ccgcgaactc
      181 cagcgcgcgg aggctggcgg gatcggggtc agccgcgcga gacgtgcggg tcatcagcga
      241 tgacgaatcg atacggccgg gcggcccatg ctccggcgta gtcgacgccg atgcgcgaac
      301 gtgtgtctat gagcgccggg agctggccga cccggatgga gatcggcccg ccgaggaggt
      361 cggatccgct caggtttcgg tccacgccca gcgcccggcc caggttcccc ggaccggaag
      421 ccagtcgtcg gatcggcacc cgttcgcccc gtcgagcctg ggccagcgtc acgccctcga
      481 ccacctcgcc cgcgcgcagg aggaccgcct cgggcttgcg tcccggtcca cagacgacat
      541 tcaggcagtc gtggatgcca tagacccggt acacgtacgc atggccgggt ggcccccaca
      601 tcggctcgtt gcgggccgtc tgcccgcgtg cggagtgcgc ggccaggtcc tccggtccga
      661 ggtaggcctc ggtctcgacc acccggaccg cgacccgtcc gtcggccgaa tcgtggatga
      721 ccaccgcccc gagcagctcg ggggcgacct ccagcgccga gcggtcgtac cagcgacggt
      781 cggccggcga ggtcagaagg tgaggaacgc cgcgatctct tccggcacga acggtcggac
      841 gacgaaaccg gcaatcggca gcagccactc gcggaaccag cccaggatga acgagtcgtt
      901 caagatgtcg tagatcgaac ccaggatccc ggccgactcg acggaagcct caccggccag
      961 ctggaagaag cttgcgagca cgacccagct gaaggtgtaa atcagggcga cgaacaggac
     1021 gcccatcacc gcgcccagga cctcgtccac ctggcgaacg aggggcagcc gctgcttgcc
     1081 gtacagggtg cggaccagca ggaacccacc gatgacgccc accgccatga gcgtgatgta
     1141 gatccacagc tcctgctcgt ccggcgtgcc gaggtgccag acgccgctca gcgagtccgc
     1201 gatcggcccc ttcagcacgc tggcgaggac gaatgcgacc aggacaacga tgcagttcag
     1261 gatgtagcgc agcagcccct gctggaaccc gaccagcacg ccgccggcca gggtgaggag
     1321 gaccacgagg tccgcgatgg tgaactgcgc gatgaattcc atgcggtcag ctctccgatg
     1381 attgcgccgc gaagggtacc agcgctgtcc accgcgacaa cgtgacgatc agcggatctc
     1441 ggctgccaga tggtggcgga gggccccgct gatcttgtcc atcgcacgct ccaccgcccg
     1501 ttcgtcggcg gccgagagcg gttggaaacg gagcgacagg ccgtacgaca cccgcccgtc
     1561 cccgatctgc ccgccgcggt acacgtcgaa cagcgtgacg tcggccagca gcggtcctcc
     1621 gttctggcgg gtcagccgca ggagctcgcc aaccggcgtc gcggcatcca ggacgacggc
     1681 gaggtcgcgg tcgaccggct gggcggccgg cacgggggcg gcgcgctccg agcgacccgg
     1741 aagatcgaac aggccatgcg ggtcgacgtg gatcgccgcg accaggggcc ggcttggcag
     1801 gccccacgcg tcggccacgg tcgggtgcac ctcccccacc gagccatacg agcgaccggc
     1861 gccgtcgacc atccgggccg cccgtccggg gtggagatgg gcatgccgtt cctccgggct
     1921 ctcgggccgg taggtcggcg ccggcaggcc gagcgcctcg tgcacggcgt ccaggattcc
     1981 ctgcaggtcg gccacgtccc agggtctggg cacctccccg ggctgtcggc ccgtcgcgtt
     2041 tccgaggagg ccgatcccca ccgtccacgc ctcccggtac ccgccgccgg cctgcgccag
     2101 ctcgtcgcct ccccccgtgg gcttgtagtg gtacgtcttg cccacctcga agatcgccag
     2161 gtcattccgg cgctggcgga cgttctcggc cagcacggcc aggaccgatg ggtatggcac
     2221 cgggcgcagg atcgcgtgct ccccggacaa cgggttggcc acccgcacga gatgggcggc
     2281 gtccgggtcg aacccggatc gccggaggtc gtcggggccg atcagggcgt gggtcagcac
     2341 ttcgtcgaga ccgagcccgg cgaggatccg ccggagcgca tggcgccggg ccgatggctc
     2401 gggccggctg agcggcggct ccgccggaga acgcgttccc tcgatccggt cgtaaccccg
     2461 ggcacgggcg atctcctcgg ccacgtccgc ggccgcgagg acgtcgttgc ggtacagcgg
     2521 cacggtcacg gccacccgtc gttcgccctc gtcggcgacc gtgaacccga gcggggtcag
     2581 ccaccgcgtc gcctcggcgc cgtccacccc gaagcccagc agccgctcgg tccgcgcggt
     2641 gtccagctcg acgacgcgcg gggcatgcgg cccggggtcg ttatcgacga tgcccgtcgc
     2701 gatccgcgcg ccggtgattg ccgcgatgag ggaggccgcc cggtcggcgg ccatccgggg
     2761 caggtccatc gagatgccct tctcatggcg catggacgcc tccgaccgga gcgccaggcg
     2821 acgggccgcg ttccggaccg tggggccgtg gaagatggcc gactcgagga tcacccggcg
     2881 agtggcgtcg gtcacctcgg tgtccgcccc gcccatgatc ccggccagcc cgatcggtcg
     2941 gtcctcgtcg gcgatcacga gcatccgttc gtcgagcgtc cgatccaggt ggtccagggt
     3001 ctcgagcgac tccccggacc gcgcccggcg cacgacgatc cggccgcccg ggatccgatc
     3061 cgcgtcgtat gcgtgcatcg gctggcccat ctcgtgcatg acgtagttcg taacgtccac
     3121 gaccggcgag accgggcgca tgccggccgc cacgaggcgc tgggccatcc aatcgggcgt
     3181 gacaccgggg ccgaccccgt cgagcagccg cgccgtgaac cgcggacaga gcgcggcgtc
     3241 ctcgatcttc acgctcaccg cgtctgccgt cggcgggtcc gcctcggtga cggtcattgc
     3301 cggccagcga acctccgccc cggtggccgc ggcgatttcg cgggcgaggc cgatcatcga
     3361 cagcgcgtca ccgcggttgg gcttgacgtc gacgtcgagc acggtgtccc ccaccgcggc
     3421 ggccagctcg gtgcccacct cgtgctcgtc ggccgtgccc aggatcaaga tcccgtcgcc
     3481 gtcgtcacca aggcccagct cgatcgggct gcacagcatc ccgttcgaca ctgcgccgcg
     3541 gatcttcgtg cgctcgatcc tccggccgcc gggaagcgtc gagccgggaa ccgcgaccgg
     3601 gacgagctgg ccgaccacaa ggttctgcgc cccgcacacg atctcgagcg ggtcgctcgc
     3661 gcccacgtcc acgttggtca gccacagggt gtcggcgttc gggtggcggt caacttcgag
     3721 cactcgaccg acgatcacgc ccgaccactc gccgccggcc ggctccacgg tcttcacctc
     3781 gaatccgagc agggtcagcc gctcggcgag ctgggcgggc gccaactcca ggtcgacgta
     3841 ttccgccagc caggtcaggg ggacgcgcat cgtcagcgtc ccccgctgaa ctgctcgagg
     3901 aagcgcaggt cggcgctgag caggaggcgc aggtccggga tgccgtgccg cagcagggtc
     3961 agtcgatcga ggcccatccc gaacgcgtat ccctggaact cgtcgggatc caggccaccg
     4021 ttgcgcagga cgacggggtg gaccatcccc gcgcccagga tcgtcatcca accgctccgg
     4081 ccgcaggccg gacagcccgg cccgccacag accaggcagc cgatgtcgaa cgccgcgctg
     4141 ggctcggtga acgggtagta gccgggccgg aagcgggtcg tggtcccgtc cccgaacatc
     4201 gcgcgggcga actcctcgag catgcccttc aggtcggcca ggctcgtggt ccgatccacg
     4261 acgaggccct ccacctgaaa gaactcgaac ccatgcgcgg catccacggc ctcgtaccga
     4321 tagcagcgac cgggcatgag cacccggatc ggcggcgtta gctcgcgcat ggcccgaatc
     4381 tggaccggcg aggtgtgggt ccgcaggatc gtcccgtcgg ccgcgggccg cggctcgccg
     4441 gcctcgaccg atccgggctc ggccacgtac agggtgtccc acagatcgcg ggacggatga
     4501 tcgggtggga tgttcaggag ctcgaagttc atgaggtcgt tctcgatctc cgggccttcg
     4561 aacacctcga agccgaaccc atggaagatc tcgcgcaggt cgcgctccac ggtcatcagc
     4621 ggatgaaggt ggccgaggcg tgccgggcga cccggcgccg tcatatcgag cgcctcgccg
     4681 gccagctgca ggtccagctc gcccgcggcc agcgcccggc cccgttcggt gatcgccgct
     4741 tcgagcgcga cccgcgcctc gttggcggcc gcacccgcgg cgggacgatc cgcgtcgggc
     4801 agggcgctca gcccgcgaag cgccgcggtg aggctgcccg atcggccgag gacggccacc
     4861 cgaatggcct cgacatcggc cgcgtcggcc gccgcggcga gcctggcctc ggcatcggtc
     4921 cggacccgcg cgacctcggc cagcagggcc gcggcgtcag tcggggtgct catcggtcag
     4981 gccatctcca tcggtcaggg ggcattgtga gtgaaacaaa cgaccccgct ccgagatccg
     5041 gagcagggcc gtgggaccga cgcggaggcg tcggtcagct ggtaaaggtg gccttggaca
     5101 tctcgaccag gtgcccgaag gcggtcggct cgcgcacggc gagatcggcg agctgcttgc
     5161 ggttgacctc gatgccggcc gacttcaggc cggccatgaa ccggccgtag ggcatgccgt
     5221 gggaccgcga ggcggcgttg atccgctgga tccacagccg gcgcatgtcg cgcttccggt
     5281 cacgtcggtc gcgataggcg tagcgcatgg cgtggaacag cgcttcgcga gccggccgga
     5341 tgagcttgga gcgggtcatc cgccgaccct cggcctgctt cagcaggcgc ttgtgacggc
     5401 ggtgggcggg aactccgcgc tttacgcgag gcatttcaga tccctattcg tacggcatca
     5461 gccgcttgag ctgggcggca tgggtcgagc tgacctgggc catcccggcg tagcggcgct
     5521 tgcggcggct ggacttgatc tggagatggt ggccccgcca ggccttgggt cggagccact
     5581 tgcccgaacc cgacttgcga taccgcttgg cggtgccctt gtgtgtcttc attttcggca
     5641 cgatggtttc tgtcccctgt cgttatgccg gcgtcgcggc cggcggctcc tcggccactt
     5701 cgggcacctg gcccttggcc tggagcgcct cgccgatcgt ctcgccttcg gccgcggcct
     5761 cgccgtcggt gaggtccggc tggtcgtcat gtcggtcggc cttggcggtg ggcggagcca
     5821 tcacgatgaa caggctccgg ccctccatga gtggcgcacg ctcgatcacc ccgtggtcgc
     5881 cgatcatctc ggcggccttg tcgagcaggt tgcgcccgag ctgtggatgg ttgagctccc
     5941 ggccccggaa ctggaccgtg accttgaccc ggtcgccgtc gtcgagaaac tcggacgcgc
     6001 ggcgggtctt ggtcgcgagg tcatgggtgt cgatcttgac ccggaaccgg atctctttga
     6061 aggtctgcga cttctgcttc ttcttcgttt cgcgctcgcg cttctggatc tcgtacttgt
     6121 actgaccgta gtccaggatc ttgcagaccg ggggaactgc cgtgggtgcc acctcgacca
     6181 gatcgaggcc gcgttcctgt gccagcgcga gtgcgtccgc ggtctgcatg accccgagct
     6241 gctggccctc gtcgtcgatg acccgcacct ctttgatacg aatcatgtcg ttgacgcgga
     6301 gttccctggt tatcggtctg tgctcccttc aacacatgag ccacggtccg ctgacggacg
     6361 cgtggcccac atctgcgcga ttgccggccg actatacacc gcggtcaaaa aatgggtcaa
     6421 cgagcctcag tccgcgtcct cccagtcgga ccacgggacg tggtgctcct cgaggtaggt
     6481 atgcaccgcc tcgcccatcg tgcgcgggaa gggcggttgc gccagctggg cctcgagacc
     6541 gtagcggacg accttgtcct tgaccggccc cttcagctcg gcgaaggaca gaccgatccc
     6601 cactcgattc agctggtcga tgagttcgga cagcatgtcc tccgccgtgg aatcgacgtc
     6661 ggtcatgggt tcggcggcaa ccaccactcg gcggattggt ggttcggccg cctcgatggc
     6721 atccagcacc gcttcgcgga aacggtcagc gttggcgaag aacagcggcg cgtcgaagcg
     6781 gaacaggacg agccccggga tctggcggcc ctcggggtgc cgctcgcggt cgtggtatcc
     6841 cttcagtccg tccacccggc cgagcaccgc ggtgtgcgga tgccaggctc gccacaacaa
     6901 cgttgcggcc gacaagccaa ccgcgattcc gatgccggcg atgaccccta ccagcgccac
     6961 accaagcaga gcggcaatgg cgagcccgaa atcggcgcga ttgatggcga acaatcgccg
     7021 cagcaccgcg aggtcgatga ggccgatcac ggcgaccatc acgatcccgg ccagggtggc
     7081 gctggggagg ggggccagga ccccggtggc gaagacgaga acgatgccga tcgcgagggc
     7141 ggccgtgacc ccggtcagct gggtccgtgc gccagccgcc tccgccgcgg gtgtccgcga
     7201 cgagctgctc gagatgggga acccttgaaa cagcccggcg gcgacgttga ccgcccccag
     7261 ggcaaacagc tcctggtcct ggtcgacccg gtcgccgagg cgcgtcgcgt agctccgaga
     7321 taggacgctc gtatcggcaa acgcgacgag ggagatcgcc agggccgcga ccgcgatatt
     7381 cccgacctcg accaggtcga aggcggggac ggcgaattgg ggcagcccgg atgggacggc
//
LOCUS       DASYGU010000229         7459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_550380, whole genome shotgun sequence.
ACCESSION   DASYGU010000229 DASYGU010000000
VERSION     DASYGU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550380"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VGO32_06435"
     CDS             <1..1692
                     /locus_tag="VGO32_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV7200422.1"
                     /translation="FTFRVQREMARILAAMGVPDPEQAVRTGGYRVITTLDYPLQQVA
                     RSEVQRWVAALADKNVHNGALVAIDSATGEIITYIGSIDYYNQTDPRVQGQFDVAGLA
                     RRQPGSAFKPIVYTSAFKARQATVSTMFVDALTNFGNDQFKYEPTNADIKEHGPVLAM
                     DSLRYSLNIPSVQMQGLVGPDVTATFAESLGIASYDYIMAQDPGLSLGLGTVPVNLTN
                     FTQGYSVFAQQGTLHPATAILEIQDRDGNVVYSREQNGPETTTPMTPAEAYLTHWIIE
                     GNTNPATNLLWGPRAQLFDVHGQRFQAGFKTGTTDDFKDVSGMGYIPGSLTVGVWMGN
                     NNQEPMSNALGQGLFSADGPLYLWHDFMDRAVNEPWDWNGHTPVVSQDFARPAGVVMA
                     NVCRFSGMTPGGCGQTISVPFLEGTLPPKDNVHHADQWHPGGCFDIVQYVQQAGRPQN
                     WVNAAASWADKVNNGRRASSGSPYAVSPLYGNGGFGGPFCGQVKATPKPTPSPEPSNG
                     CPFPPPFCPTPSPQSNAPVAGGGPPIDAGLLIPAFAIPLLGGGASYLLRLTRRKH"
     gene            1709..2965
                     /locus_tag="VGO32_06440"
     CDS             1709..2965
                     /locus_tag="VGO32_06440"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV7200423.1"
                     /translation="MARSVLTLILAGGEGERLSVLSQVRAKPGVPFGGKYRIIDFSLS
                     NVVNSGLTDVGILTQYAPRSLIDHIGVGRPWDLDRSRGGVSLLQPYVGRGRKREWYRG
                     TADAVLQNLEYIEDRDPELVLVLAGDHVYKMDYRPFIALHRARDADLTVAVRRVPLAD
                     ANRFGILEVGDEGRVTHFVEKPENPTSNLVSMGVYAFRWPLLREVLSAERLDFGRDVL
                     PAMVQSGRAVHAYEYQGYWQDIGTVDAYWQASMDLVADQPPLDLYETGWLIYTRSEER
                     APAHIGADARVTRSLVSHGCIIEGAVDRSILSPGVRVGAGAVVRESIVMFDTEIGPGA
                     VVDRAIIDKECVIGANAHVGDGRVLRPNRSEPERLFSGLTLVGKQAQIPAHTRIGRNC
                     RIDPSVTEADFPRRRVAAGDTVVHAG"
     gene            3009..3533
                     /locus_tag="VGO32_06445"
     CDS             3009..3533
                     /locus_tag="VGO32_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200424.1"
                     /translation="MAPPLDAWLDDLGLVPVGQTAEPHELALSRDVILDGERRFDLRV
                     TIAWAAGMGLHLWAYYGLDSMELPRRTLLRLLGANAELPFVKFAVTEEDRPMLIAELP
                     PIAVDRDELGRALARLVVVADRLLTETAPAIADRGRLPDWTGRVTRNPALLDRYRAEV
                     EATMPEWPPSEGNR"
     gene            3530..5503
                     /locus_tag="VGO32_06450"
     CDS             3530..5503
                     /locus_tag="VGO32_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200425.1"
                     /translation="MKAGPMRAGPMRGGRVALSLIAAIAAFGAATAVPGGVAAAEYEM
                     TTHANYLVDEDVAQVAITVDIAFKNTFAPPAGQVSLFRTIRVAIHDHAAAVSARDATG
                     ALTVTVAVSGGVNVATITPRAPVRYGQTATLTLAYELHDGDDPTIRIGEHLVSFPAWG
                     FGTQSEVRVDLAADYEVRVDGDALEATVEDDRTILTSGAIDDPTHWLTHVGATREPTY
                     DTLQEAVALEGGTADLQVKHWEDDPEWGQATMALVVDALPLLEETFGLPYPANGPLVI
                     TETVTAGGPDVEGDNGELAVGFTEPPFTVLHQVAHRWAGESLTGDRWLLEGLASWAAG
                     QVSAELEIDLPYDPAAVAADMSENAFPLAEWNGDDHPPAVDAWAYAASWDLINDAATQ
                     AGPAAFQMALARMEAGLDGYDPLTDTPPDPAAEPVTITSRSYLDQLDAVTTEPVVEAL
                     ATPVLGEDGAAALPARAEARSAHDALLAAAGDWGDPDPVRAAMVEWRFEDAMTEMAIA
                     SGWLAERDVLLREIAGVGMTAPERLSAAYRLHGGGGEAWIEIEAERAVVNAYDDAAAA
                     VAAGIDPVARVGLMLGPSPEMRVANAAADFAAGDLRAAADELAALTHDLDTATASGLV
                     RILGLIVAVAAAVLLGMMAMRRRRTGTNYTPEP"
     gene            5500..6564
                     /locus_tag="VGO32_06455"
     CDS             5500..6564
                     /locus_tag="VGO32_06455"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV7200426.1"
                     /translation="MSGEDSTDRATATDASDGAGRFEPLDEVLSGQTADVYFERAQAI
                     LKAEHLDPVVVMEVFSRDAGILCGAAEVHAYLGKVLGAGNGHDPMPIVDSLSDGDPFG
                     SKEVVMRIEARYSAFGLYETAVLGTLAQCSGWATAARAIVEAAAPIPVIGFGARHVHP
                     SVADQMDYASVIGGCIGASTPAGARMAGLSPTGTMPHALVLIFGDTVRAAEAFDRHID
                     PEVPRIVLVDTFKDEAEEALRVAEALGDRLWGVRLDTPSERGRVTPDLVKEVRARLDL
                     AGHDHVKIVVSGGLDAERIGVFKEALAPVDSFAVGSAISDASPIDFTGDLKEIDGKPI
                     AKRGRIPGRTENHRLKRLAL"
     gene            complement(6561..7091)
                     /locus_tag="VGO32_06460"
     CDS             complement(6561..7091)
                     /locus_tag="VGO32_06460"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7200427.1"
                     /translation="MEERTVESRVAYAGRLLTMRVDEVVMADGRPATREIIEHPGAVG
                     ILAWDGERMAAVRQWRQAAGRETLEIPAGTRDPGEEPRQTAERELAEECGVSAGTWEA
                     GPAFWTAPGFCTEYLSLWLATDLSPVDVTGPEDEALERTWLTLDEALTAVADGAINDA
                     KSLVGIFWLARRLGRG"
     gene            7146..>7459
                     /locus_tag="VGO32_06465"
     CDS             7146..>7459
                     /locus_tag="VGO32_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200428.1"
                     /translation="MISVHGLERRFRTVTAVYDLSFSVPAGQVYGLLGPNGAGKTTTI
                     RMLAGLIAPTAGTAEIAGHPLGSDNAAIRAMTGILTESPGLHDRLTARQNLAYYGRLY
                     GLT"
BASE COUNT         1158 a         2706 c         2527 g         1068 t
ORIGIN      
        1 ttcacgttcc gagtccagcg cgagatggcc cgcatcctgg ccgcgatggg cgtgcccgac
       61 cccgagcagg cggtccgcac cggtggctat cgggtgatca cgaccctgga ctacccgctc
      121 cagcaggtcg cccggtcgga ggtccagcgc tgggtcgcgg ccctggctga caagaacgtg
      181 cacaacgggg cgctggtcgc gatcgactcg gcgactggcg agatcatcac ctacatcggc
      241 agcatcgact actacaacca gaccgaccca cgggtccagg gccagttcga cgtcgctggc
      301 ctggcccgcc gccagccggg atcggcgttc aagccaatcg tttacacgtc ggccttcaag
      361 gcgcggcagg ccacggtctc gaccatgttc gtggacgcgc tgaccaactt cggtaacgat
      421 caattcaagt acgagcccac gaacgccgat atcaaggagc acggcccggt cctcgccatg
      481 gactcgctgc ggtactccct gaacatcccg tcggtccaga tgcagggcct ggtcgggccg
      541 gacgtgacgg ccaccttcgc cgagtcactc ggcatcgcca gctacgacta catcatggcc
      601 caggatcccg gcctttccct gggcctcggc accgtcccgg tcaacctgac caacttcacc
      661 cagggctaca gcgtcttcgc ccaacagggc acgctccacc cggcgaccgc gatcctcgag
      721 atccaggacc gcgacggcaa cgtggtctac tcccgcgagc agaacgggcc ggagacgacg
      781 acgccgatga ctccggccga ggcgtacctc acccactgga tcatcgaggg caacaccaac
      841 cctgccacca acctgctgtg gggcccgcgc gcgcagctgt tcgacgtcca cggccagcgc
      901 ttccaagcag gattcaagac cgggacgacc gacgacttca aggacgtgtc gggcatgggc
      961 tacatccccg gcagcctcac ggtcggggtg tggatgggca acaacaacca ggagccgatg
     1021 tcgaacgcgc tgggccaggg cctcttctcg gccgacgggc cgctgtacct gtggcacgac
     1081 ttcatggacc gcgcggtcaa cgagccgtgg gactggaacg gccacacgcc ggtcgtgagc
     1141 caggacttcg cccgcccggc gggcgtggtc atggccaacg tctgccgctt cagcgggatg
     1201 actccgggtg ggtgcggcca gacgatctcg gtcccgttcc tggagggcac tcttccgccc
     1261 aaggacaacg tccaccacgc cgaccagtgg cacccgggcg gctgctttga catcgtccag
     1321 tacgtccagc aggccgggcg gccccagaac tgggtgaatg ccgctgccag ctgggcggac
     1381 aaggtcaaca acggccgccg ggcgagctcc ggcagcccgt acgcggtctc cccgctgtac
     1441 ggcaatggcg gctttggtgg tccgttctgc ggccaggtaa aagcgacgcc caagccaacg
     1501 cccagcccgg aaccatcgaa cggctgcccc ttcccgccgc cgttctgccc gaccccgagc
     1561 ccacagtcca acgcacccgt ggccggcggt ggcccaccca tcgacgctgg cctcctcatc
     1621 cccgccttcg cgataccgct cctcgggggt ggagcctcat acctgttgcg cctgacccgg
     1681 cgcaagcact gagccaccac taccgcgcat ggcccggtcc gtcctgaccc tcatccttgc
     1741 cggtggggag ggggaacgcc tgtcggtgct gtcccaggtg agagcgaagc cgggcgtgcc
     1801 gttcgggggg aagtaccgga tcattgattt ctcgctgtcg aacgtcgtga actcgggcct
     1861 caccgacgtg ggcatcctga cccagtacgc cccacgctcg ctcatcgacc acatcggcgt
     1921 gggccggccg tgggacctcg accggagccg cggcggggtg agcctgctcc agccgtacgt
     1981 cggccgtggg cgcaagcgcg agtggtaccg cggaaccgcc gatgcggtcc tccagaacct
     2041 ggagtacatc gaggatcgcg atccggagct cgtcctcgtc ctggccggcg accacgtgta
     2101 caagatggac taccgcccgt tcatcgccct ccatcgggcg cgtgacgcgg atctcacggt
     2161 cgccgttcgc cgcgtcccgt tggccgatgc gaatcggttc gggatcctcg aggtgggcga
     2221 cgagggccgg gtgacgcact tcgtggagaa gccggaaaac ccgacctcca acctggtgag
     2281 catgggggtc tacgcctttc gctggccgct cctgcgcgag gtgctctccg ccgagcgcct
     2341 ggacttcggc cgcgacgtgc tgccggccat ggtccagtcc ggccgggcgg tccatgccta
     2401 cgagtaccag ggctactggc aggacatcgg gacggtggac gcctactggc aggcgagcat
     2461 ggacctcgtc gccgaccagc cgccactgga tctgtacgag accggctggc tgatctacac
     2521 ccgcagcgaa gagcgagcgc cggcccatat cggtgccgat gcccgagtca cccgcagtct
     2581 cgtcagccac ggctgcatca tcgaaggagc cgtggatcgc agcatcctgt cgcccggcgt
     2641 gcgggtgggg gccggcgcgg tcgtccggga gtcgatcgtc atgttcgaca ccgagatcgg
     2701 tccgggggcg gtcgtggacc gggcgatcat cgacaaggaa tgcgtgatcg gcgcgaacgc
     2761 gcacgtgggt gacggccgag tcctgcgccc caaccggagc gagcccgagc gcctgttcag
     2821 cggcctgacc ctggtgggca agcaggcgca gatcccggca cacacccgga tagggcgaaa
     2881 ctgccggatc gatccctccg tgaccgaggc cgacttcccc cgacgccggg tggcggcggg
     2941 ggacacggtg gtgcacgctg gttgagctcg gcgcggcaag cgccggctcc aacgcgacga
     3001 ccggcgacat ggccccgccc ctcgacgcct ggctcgacga cctgggcctg gtcccggtcg
     3061 gccagacggc agagccgcac gagctggccc tgagccgcga cgtcatcctc gacggggagc
     3121 gccggttcga cctgcgggtc acgatcgcgt gggcggcggg aatgggtctc cacctgtggg
     3181 cctactacgg gctcgactcg atggagctgc cccgacggac gctgctccgg ctgctgggcg
     3241 ccaacgccga acttccgttc gtgaagttcg ccgtgaccga ggaggaccgg ccgatgctca
     3301 tcgccgagct gccaccgatc gcggtcgacc gcgacgagct cggccgagcc ctggcccggc
     3361 tggtggtggt cgccgaccgg ctcctgaccg agaccgcccc ggccatcgcg gatcgcggcc
     3421 gattgccgga ctggaccggg cgcgtgactc gcaacccagc actcctggac cgataccggg
     3481 ccgaggtcga agccacgatg cccgaatggc caccgtcgga ggggaaccga tgaaggccgg
     3541 accgatgcgc gctggaccaa tgcggggcgg tcgggtggcc ctgtcgctca tcgcagcgat
     3601 cgcggcattc ggcgcagcca ccgcggtccc cgggggtgtg gctgccgccg agtacgagat
     3661 gacgacccac gccaactacc tcgtggacga ggacgtcgcc caggtggcga tcaccgtgga
     3721 catcgccttc aagaacacgt tcgccccgcc cgcgggccag gtcagcctgt tccgcaccat
     3781 ccgggtcgcg atccacgacc acgcggcggc ggtgtccgcc cgcgatgcga ccggcgcgct
     3841 gaccgtgacg gtggcggtgt ccggcggtgt caacgtggcg accatcaccc cccgcgcacc
     3901 ggtcaggtat ggccagaccg cgacactcac gctggcatac gagctgcacg acggggacga
     3961 ccccacgatc cggatcggcg agcacctcgt gagcttcccg gcgtggggat tcgggaccca
     4021 gagcgaggtc cgggtggacc tcgctgccga ctacgaggtc cgggtcgacg gcgacgcgct
     4081 cgaggcgacg gtcgaggacg accgcacgat cctgaccagc ggcgcgatcg acgaccctac
     4141 gcattggctc acgcacgtgg gcgcgacgcg cgaacccacg tatgacacgc tccaggaggc
     4201 cgtcgcgctg gagggtggca ccgccgacct ccaggtcaag cactgggagg acgacccgga
     4261 gtggggccag gccaccatgg cgctggtggt ggacgcgctg ccgctgctgg aggagacctt
     4321 tggccttccg tacccggcca acgggccgct ggtcatcacc gagacggtga ccgccggcgg
     4381 accggacgtg gagggagaca acggagagct ggcggttggc ttcaccgaac ccccgttcac
     4441 cgtcctgcac caggtcgcgc accgctgggc cggcgagtcg ctgaccggcg accgttggct
     4501 gctcgaggga ctcgcgtcgt gggccgcggg tcaggtctcg gccgagctcg agatcgacct
     4561 gccctacgat cccgccgccg tggccgcgga catgtcggag aacgcattcc cgctcgccga
     4621 atggaacggc gacgaccatc cgcccgcggt tgacgcgtgg gcatacgccg cctcctggga
     4681 cctgatcaac gacgctgcca cccaggcggg tcccgcggcc ttccagatgg ccctcgcccg
     4741 gatggaagcg ggcctggacg gctacgaccc gctgaccgat acccctcccg accccgccgc
     4801 ggaaccggtc acgatcacct cacgttcgta cctggaccag ctggacgcgg tgaccaccga
     4861 gcccgtggtc gaggccctcg ccaccccggt cctgggcgag gacggcgcgg cagccctgcc
     4921 ggcccgagcg gaggcgcgga gcgcgcatga cgcgctcctg gcggccgccg gcgactgggg
     4981 cgatccggat ccggtccgcg cggccatggt cgagtggcgc ttcgaggacg ccatgaccga
     5041 gatggccatc gcgagcggct ggctcgccga gcgcgacgtg ctgctgcgcg agatcgcggg
     5101 cgtcggcatg accgcgcccg aacggctgag cgcggcatac cggctccatg gaggcggtgg
     5161 agaggcgtgg atcgagatcg aggccgagcg ggcggtggtg aacgcctacg acgacgcagc
     5221 cgcggccgtc gcggcgggga tcgaccccgt cgcgcgggtg ggcctgatgc tgggccccag
     5281 ccctgaaatg cgggtcgcaa acgcggcggc cgactttgcg gccggcgacc tgcgggcggc
     5341 ggccgacgag ctggcggcgc tgacccacga cctggatacc gccaccgcca gcggcctggt
     5401 gcggatcctc ggcttgatcg tggccgtggc cgccgccgtg ctcctgggca tgatggccat
     5461 gcgccgacgc cggacgggga ccaactacac tcccgagcca tgagcggcga ggactccacc
     5521 gaccgcgcca ccgcgaccga tgcctcggac ggggccggcc ggttcgagcc cctggacgag
     5581 gtcctgagcg gtcagaccgc ggacgtctac ttcgagcggg cccaggcgat cctgaaggca
     5641 gagcatctcg acccggtggt cgtgatggag gtcttctcgc gcgacgccgg gatcctgtgc
     5701 ggagcagccg aggtccatgc ctacctgggc aaagtcctcg gcgccggcaa cgggcatgac
     5761 ccgatgccca tcgtcgactc cctgtccgac ggcgacccct tcggatcgaa ggaggtcgtg
     5821 atgcggatcg aggcccgcta ctccgctttt gggctgtacg agaccgctgt cctgggcacc
     5881 ctggcccagt gctccggttg ggcaaccgcg gcccgcgcga tcgtcgaggc cgcggcgccg
     5941 atcccggtca tcggcttcgg ggcgcgccac gtccacccca gcgtggcgga ccagatggac
     6001 tacgccagcg tgatcggcgg ctgcatcggc gcgtccaccc cggccggggc gcggatggcc
     6061 ggcctgtcac cgacggggac catgccccat gcgctggtcc tcatcttcgg cgataccgtc
     6121 cgcgcggccg aggcgttcga ccgccacatc gaccccgagg ttccccgcat cgtgcttgtg
     6181 gataccttca aggacgaggc cgaggaggcg ctccgggtcg ctgaagcgct gggcgatcgt
     6241 ttgtggggcg tccggctgga cacaccatcc gagcgggggc gggtcacacc cgaccttgtg
     6301 aaggaggttc gtgcccgcct ggacctggcc ggacatgacc acgtgaagat cgtcgtctcc
     6361 ggcgggctgg acgccgagcg gatcggcgtg ttcaaggagg cgctcgcgcc ggtcgactcg
     6421 ttcgcggtcg gttcggccat ctccgacgcc agcccgatcg acttcactgg cgacctgaag
     6481 gagatcgacg gcaagccgat cgccaagcgc ggccggatcc cgggccgaac cgagaaccac
     6541 cggctgaagc ggctcgccct ctagcctcga cccaggcgcc gcgccagcca gaagatcccg
     6601 accagcgact tcgcgtcgtt gatcgcgccg tcggccaccg cggtcagcgc ctcatccagg
     6661 gtcagccagg tgcgctcgag cgcctcatcc tccggaccgg tgacgtcgac cggcgacagg
     6721 tcggtggcaa gccacaggct caggtactcg gtgcagaacc cgggcgccgt ccagaacgcc
     6781 ggtccggcct cccacgtgcc cgcgctcacc ccgcactcct cggccagctc acgctcggcg
     6841 gtctgccggg gctcctcccc cgggtcgcgg gtcccagccg ggatctcgag cgtctcccgc
     6901 cccgccgcct ggcgccattg gcgcaccgcg gccatccgtt caccgtccca cgccaggatc
     6961 cccaccgcgc cgggatgctc gatgatctcc cgcgtggcgg gccgtccatc ggccattacc
     7021 acctcgtcca cccgcatcgt gagcagccgt cccgcgtacg caacccgaga ttccaccgtt
     7081 cgctcttcca tgacccgatc ttgccattcc ggaccccccg cctgcccctg ctagcatcga
     7141 tgctcgtgat ctcggtccac ggtctcgagc gtcggtttcg cacggtcacc gccgtgtacg
     7201 acctgtcatt cagcgtgccc gcaggccagg tctacggact cctcggtccg aacggcgcgg
     7261 gcaagaccac caccatccgg atgctggccg ggctcatcgc gcccactgcc ggcacggccg
     7321 agatcgcggg acatccgctc ggatcggaca acgccgccat ccgggccatg accgggatcc
     7381 tgaccgagag ccccggcctc catgaccggc tcactgcgcg tcagaacctc gcctactacg
     7441 gccgcctgta cggcctgac
//
LOCUS       DASYGU010000230         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1579081, whole genome shotgun sequence.
ACCESSION   DASYGU010000230 DASYGU010000000
VERSION     DASYGU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579081"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VGO32_06470"
     CDS             <1..338
                     /locus_tag="VGO32_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7200429.1"
                     /translation="SDDPEASGNFLADDVEWHEIGRSDAIHGKAALRERMMGAGKPEW
                     EITGGETHDVIGGDDHTVALLSAHAVWGDKTIDYRVVEIFHVKDGKITGRWAFSDDTD
                     AINTFFAGT"
     gene            417..830
                     /locus_tag="VGO32_06475"
     CDS             417..830
                     /locus_tag="VGO32_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7200430.1"
                     /translation="MSHHEDGHDNRASDASLKVLRQALDAFNRGDSEAAAALLSDDVE
                     WHEIGRADPIRGKAALAERFAGALPEWQITTEVHDMLANEEHAIALVTATATMGGKSF
                     TYRTAEIYHVRDGKVTARWAFSDDTGAVNEFFAGT"
     gene            complement(847..1143)
                     /locus_tag="VGO32_06480"
     CDS             complement(847..1143)
                     /locus_tag="VGO32_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200431.1"
                     /translation="MAEPEEVPEETRADEHEDEAADDDASGRLPSFDGVNLSIGEFMA
                     GLEQKILHNRPPAVVVVEEHRRGETVATNGLVIDDLPTEPLDRPEPPDTSGAKL"
     gene            complement(1153..1728)
                     /locus_tag="VGO32_06485"
     CDS             complement(1153..1728)
                     /locus_tag="VGO32_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200432.1"
                     /translation="MTTTFIRPNRLGMLAIAFLAAAAVLATTAVTSVRAGVPPPACAI
                     LISSVSSPVPAETAVIIVDEPVQVTGTGFTPNATLDITVVFNGVPQATFPQATDGSGN
                     FLLTGSLTQAQIGAWTLTAADGQLCNDTVSFTVVAAAVATPTPEALPNAAMIPAPAST
                     GGGAGLAFAVLGSALIGLALVTLRTRKVTER"
     gene            1851..3242
                     /locus_tag="VGO32_06490"
     CDS             1851..3242
                     /locus_tag="VGO32_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEV7200433.1"
                     /translation="MPSPESRLRAERVSTAAAATAQAPIATHADLTVAANRDPIRLLL
                     AQETTRIPELVPVRHGRMLASPFAFFRGAALPMAADLAQLPSSGLWVQLCGDAHLANF
                     GAFASPERRLMFDINDFDETLPGPFEWDLKRFAASLAIAGRANGFSTKDRRRVMVNVL
                     GRYRSSMQKFAAMPLLDVWYAHLDLEDLIVKFRSQHQHKSAKQVEATVAKARTRDQLQ
                     VSNKMTHVVNGVPQIVANPPLVVPIEDLTDLDADAVAAFLRTQERRYRRSLEGDRRFL
                     LDRFRLVHAARKVVGVGSVGTRDWILLFVADDESVLFLQYKEAQPSGLSGYLGGSAFD
                     NQGERVVQGQRLIQAVSDIFLGWQRVDAPQSRTQDYYVRQLRDGKVSQSIEALSASGM
                     IQYGRLCAWTLARAHARTGDRVALTEYMGSSDHFENALAEFGEAYADQNERDFAALAD
                     AVASGQVEAVRGL"
     gene            complement(3239..>4306)
                     /locus_tag="VGO32_06495"
     CDS             complement(3239..>4306)
                     /locus_tag="VGO32_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7200434.1"
                     /translation="AQFGPRELPSGETRGISADVIVNDSQVAIVTVVVPVGTDQRPFL
                     RVFNVALLVAGLASVAVIAGVSVWMARRQTRPLHDVAAAATRLGAGDLSARATGGGDR
                     ESQELAGAFNTMADRLEQSEALRRRAATDMAHDLATPATVLQTQLQAMIDGVVTKSKR
                     NLQAAAASSAALGSVIVQMGELASAEAAPLQAHPLRVAVDGIVAEAANALDGLYRERG
                     VALTIEPVPPDLMARADPAHLGRALRNVLTNAAQHTPSGKGVRVTATAAPATLPDAPT
                     RVQIRVADEGPGIPAEDLPHVFERFYRSDPARTSGGTASGGAGIGLTIARELLAPSAG
                     VISVERTGADGTTVLIDLAPA"
BASE COUNT          698 a         1473 c         1502 g          633 t
ORIGIN      
        1 aatcggacga ccccgaagcg tcgggaaact tcctggccga tgacgtcgag tggcacgaga
       61 tcggccggag cgacgccatc cacggcaagg cggccctgcg cgagcggatg atgggtgccg
      121 gcaagcccga gtgggagatc accggtggcg agacgcacga cgtgattggt ggcgacgacc
      181 acaccgtggc cctgctcagc gcgcacgcgg tctggggaga caaaacgatc gactaccggg
      241 tcgtcgagat cttccacgtc aaggacggca agatcaccgg ccgctgggcg ttctcggacg
      301 acaccgacgc catcaacacg ttcttcgccg ggacctgacc gcggccccgg gaggggcctc
      361 acggggatcc gcgggcgtac gatggccgtc caccatcgcc ccaaaggatc ccaaccatgt
      421 ctcatcacga agacggccac gacaatcggg cctctgacgc atcgctcaag gtgcttcgcc
      481 aggcgctcga cgccttcaac cgcggtgaca gcgaggccgc ggcggcgctg ctctcggacg
      541 acgtcgaatg gcatgagatc ggccgcgctg atccgatccg cgggaaggcc gcgctggcgg
      601 agcgattcgc cggcgccctg cccgaatggc agatcacgac cgaagtccac gacatgctgg
      661 ccaacgagga gcatgccatc gccctggtga ccgccaccgc cacgatgggc ggcaagtcgt
      721 tcacctaccg caccgccgag atctatcacg tgcgcgatgg caaggtcacc gctcggtggg
      781 cgttctccga cgacaccggt gccgtcaacg agttcttcgc cgggacctga ccgaccgggg
      841 cgagcgctag agcttcgccc ctgacgtatc ggggggctcg ggccgatcaa gcggctcggt
      901 gggcaggtcg tcaatgacca gcccattggt ggccacagtc tcgccacgcc tgtgctcctc
      961 cacgaccacg accgccggtg gacggttgtg gaggatcttc tgctccaggc cggccatgaa
     1021 ctcgccgatg gacaggttca cgccgtcgaa ggagggcagg cggccggacg cgtcatcgtc
     1081 cgccgcctcg tcttcgtgct cgtctgcgcg tgtttcctca gggacctctt caggttcggc
     1141 cacggaccga tcctagcgct cggtcacctt ccgggtgcgg agcgtgacca gggcaagacc
     1201 gatcaacgcc gagccaagca cggcgaaggc caggcccgct cccccaccgg tcgaggcggg
     1261 agccgggatc atggccgcgt tcggcagagc ctcgggcgtg ggcgtggcca ccgcggcggc
     1321 cacgaccgtg aagctcacgg tgtcgttgca gagctgtcca tcggccgcag tcagcgtcca
     1381 tgcgccaatc tgcgcctgtg tcagcgaacc ggtcagcagg aagttgccgc tcccatcggt
     1441 cgcctggggg aacgtggcct gcggcacgcc gttgaacacg acggtgatgt cgagcgtcgc
     1501 gttgggcgtg aaaccggtcc cggtcacctg gaccggctcg tccacgatga tgaccgcggt
     1561 ctcggcgggc accggcgagg acaccgagct gatgaggatg gcgcaggccg ggggcggaac
     1621 gccggcacgg accgatgtga ccgcggtggt ggcgagcacg gcggcggcgg ccaagaaggc
     1681 aatggcaagc atgccaaggc ggtttgggcg aatgaacgta gtcgtcatgg aagctcctgc
     1741 aggacgaaaa atgacccgtt tacgcccctg agcgtggcgg tcaggcgtgg gaggcgacaa
     1801 taggagatct ggccggccca tgggtccggt gacggcagta cgctgggctc atgcccagcc
     1861 ccgaatcgcg cctccgcgcc gaacgagtgt ccacggccgc cgcggcgacc gcccaggcgc
     1921 cgatcgcgac gcacgccgac ctcacggtgg cagcgaatcg ggacccgatc cgcctgctcc
     1981 tcgcccagga gacgacgcga atcccggagc tcgtcccggt ccgtcatggg cggatgctgg
     2041 cctcaccgtt cgcgttcttc cggggcgcgg cgctgcccat ggccgcggac ctcgctcagc
     2101 tgcccagctc cgggctctgg gtccagctgt gcggtgacgc ccacctcgcg aacttcgggg
     2161 ccttcgcctc ccccgagcga cgcctgatgt tcgacatcaa cgactttgac gagacgctgc
     2221 ccggtccgtt cgagtgggat ctcaagcggt tcgccgccag cctggcgatc gcgggacggg
     2281 caaacggctt ctccaccaag gaccggcggc gggtaatggt gaacgtgctg ggccggtatc
     2341 ggtcctcgat gcagaagttc gcggccatgc cgcttctcga cgtctggtat gcccacctgg
     2401 atctcgagga cctgatcgtc aagttccgat cacagcacca gcacaaaagc gcgaagcagg
     2461 tcgaggcgac cgtcgccaag gcgcggacac gggatcagct gcaggtgtcc aacaagatga
     2521 cccacgtcgt gaacggggtg ccccagatcg tcgccaaccc gccgctcgtg gtgccgatcg
     2581 aggacctcac cgatctcgac gccgatgccg tcgccgcgtt cctccgaacc caggaacggc
     2641 gctatcggcg cagcctcgag ggggatcgac gttttctcct ggaccgattc cggctcgttc
     2701 acgctgcacg caaggtcgtg ggcgtgggca gtgtgggcac gcgcgactgg atcctcctgt
     2761 tcgtcgcgga cgatgagagc gtcctgttcc tccagtacaa ggaggcacag ccgtccggtc
     2821 tgtcgggcta cctgggtggg agtgcattcg acaaccaggg cgagcgggtc gtccagggac
     2881 agcgcctgat ccaagcggtg agcgacatct tcctcggctg gcagcgggtg gacgccccgc
     2941 agtccaggac gcaggactac tacgtgcgcc agctgcggga cgggaaggtc tcgcagtcga
     3001 tcgaggccct gagcgcgagc ggcatgatcc agtacgggcg cctctgtgcc tggaccctgg
     3061 cccgggccca tgcccgcacc ggggaccggg tcgcgctcac cgagtacatg ggctcgtcag
     3121 atcacttcga gaacgccctg gccgagttcg gcgaggccta cgccgaccag aacgaacgcg
     3181 actttgccgc gctggccgat gcggtggcgt ccggccaggt cgaggctgtt cgcgggctct
     3241 aagccggggc gaggtcgatc aggacggtcg tgccgtcggc gcccgtgcgc tcgaccgaga
     3301 tgaccccggc gctcggcgcg agcagctcgc gggcaatggt cagaccgatg ccggcgcctc
     3361 cgctggcggt gccgccggag gtgcgcgcgg ggtcggatcg gtagaagcgc tcgaagacgt
     3421 ggggaaggtc ctcggccgga atgccgggcc cctcgtccgc gacccggatc tggacccgag
     3481 tgggagcgtc gggcaaagtg gccggtgcgg cggtggcggt gacccgaacg cctttgccgc
     3541 tcggcgtgtg ctgcgcggca ttggtcagca cgttgcgcag cgcccgaccg aggtgcgcag
     3601 ggtcggcccg ggccataagg tccggaggga caggctcaat cgtgagcgcc accccgcgct
     3661 cgcggtacag gccgtcaagc gcgttcgcgg cctcggccac gatgccgtcc acagccacgc
     3721 gcaagggatg agcctggagg ggagccgcct cggcgctggc gagttccccc atctgcacga
     3781 tcaccgatcc gagggcagcc gaggacgccg ccgcggcctg gaggttgcgt ttcgacttgg
     3841 taacgacccc gtcgatcatg gcctgcagct gggtctggag caccgtcgcc ggcgtggcga
     3901 ggtcatgggc catgtccgtc gccgcgcgcc gacggagcgc ctcggactgc tccagccggt
     3961 cggccatggt gttgaacgct ccggccagct cctgcgactc gcggtccccg ccgcccgtgg
     4021 cccgcgctga gaggtcgccg gcacccagcc gggtcgcggc cgcggccacg tcgtgcagcg
     4081 ggcgggtctg ccgacgcgcc atccagaccg ataccccggc gatcaccgcc accgacgcca
     4141 gcccggcgac cagcagcgcc acgttgaaca cccgcaaaaa cggccgctgg tcggtcccca
     4201 ccggaacgac gaccgtgacg atcgccacct ggctgtcgtt cacgatcacg tcggccgaga
     4261 ttccgcgcgt ctcgccgctc ggcagctcgc gcggtccgaa ctgggc
//
LOCUS       DASYGU010000231         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1013329, whole genome shotgun sequence.
ACCESSION   DASYGU010000231 DASYGU010000000
VERSION     DASYGU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013329"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1201)
                     /gene="ruvB"
                     /locus_tag="VGO32_06500"
                     /pseudo
     CDS             complement(153..1201)
                     /gene="ruvB"
                     /locus_tag="VGO32_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
     gene            complement(1198..1770)
                     /locus_tag="VGO32_06505"
     CDS             complement(1198..1770)
                     /locus_tag="VGO32_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV7200435.1"
                     /translation="MTDRSPVAAVILAAGLGRRFGGPKVAARLGDDTLLMRVSRIARD
                     AGLNPILAVVPRGTETPARVEAVVNADPARGQSLSLRLGIERVPSNRAALILLVDQPT
                     MPPESIAAVLAARGRRPLVVAVADDRYGPPALIEPEAFPLVMELSGDIGLREVLASRP
                     DLVEAVPVAAHPPDVDTAADLDRLEDRLGP"
     gene            1789..>1944
                     /locus_tag="VGO32_06510"
     CDS             1789..>1944
                     /locus_tag="VGO32_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV7200436.1"
                     /translation="MIDEALAAVRGWRADGREAALATVVRVEGSAPREVGAKLAVAAG
                     GEMAGSVS"
BASE COUNT          261 a          663 c          728 g          292 t
ORIGIN      
        1 ggccctcctc gctccctgga tcgccgccgc accaagggct gggcgcgtga cgcgacccgg
       61 agccggatcg tagccgcccg gaatgtggat atcgcccgtt ttctgcgcga cgccctcgga
      121 ccgcaccgga tcgtgcggaa ccgacccgaa aatcagtcga acaggccggg ttgcggggcg
      181 gccacgacgg ccgattgtgc gggtggcgtc agccccagat gggtccaggc ggttggcgtg
      241 agccgtcggc cgcgcggcgt ccgcgccagc aggccggcct ggatgaggta cggctcgacc
      301 acgtcttcga tcgtctccac cggctcgctg atggtggccg ccatggtctg gaggccaccg
      361 gtcccccgcc gtggtgctcg gcaatcgcgg tcagcagctg gcggtcgagg gtgtccaggc
      421 cgcgctcgtc gatgtccaat aaaccgaggg cggcttcgga agcggcggcc gtcgcgttgc
      481 catcctcgtc gccccggacc tgagcgtagt cccggacccg cttcaggagc cggttcgcga
      541 tccgcggcgt gccgcggctc cgacgggcaa tgagctcggc cgcgtcgtcg tcgagcggga
      601 tctccaggat gccggcgctg cggtggagga tggtgaccag gtccgcctcg gtgtagtagt
      661 ccagccgatg ggtgaccccg aaccggtcgc gcagcgggcc ggtgatgctc cctacccgag
      721 tcgtggcccc gatgatcgtg accggctcga tctggagtcg cacggtccgt gccccggggc
      781 ccttgcccag cacgatgtcg agcgcgaagt cttccatcgc cgggtacagg acctcctcga
      841 ccacgtggga caggcggtgg atctcgtcga tgaacaggac gtccccgcgc tggagcccgg
      901 tcaggatcgc ggccaggtca ccggcccgct cgatggccgg gccggaggtc gtccgcagct
      961 gagcgccaac ctctgcggcc acgatggtcg ccagcgtggt cttgcccagc ccgggaggcc
     1021 cgtagagcag gatgtgatcc accggctccg accgctgccg ggcggcgtcg atgaggatgg
     1081 ccaggttctc cttgattcgc tcctggccgg cgaactcatc gagccggcgc ggtcgaacgg
     1141 tggcctccag cgaccgttcg tcctcgagca cctcggcgtt gccgacccgt tcgatggtca
     1201 tggcccgagt ctatcctcca gccggtcgag gtcggccgcg gtgtcgacat cgggcggatg
     1261 ggccgccacc ggcaccgcct cgacgaggtc cggccgtgat gcgaggacct cccgcaggcc
     1321 gatgtcaccg ctcagctcca tgaccagcgg aaaggcttcg ggctcgatga gcgcgggtgg
     1381 cccgtaccga tcgtcggcca cggccaccac gagcgggcgt cggccacggg cggcgagcac
     1441 cgcggcgatg ctttcgggcg gcatcgtggg ctgatccacg agcaggatca acgccgcgcg
     1501 gttcgagggg acccgctcga tgccgagccg gagcgagagg ctctggcccc gggctggatc
     1561 ggcgttcacg acggcctcga ctcgggccgg cgtctcggtt ccgcgcggga cgacggccag
     1621 gatgggattc agaccggcgt cgcgggcgat gcgtgagacc cgcatcagga gcgtgtcgtc
     1681 acccagtcgc gcggcgacct ttggtccgcc gaagcggcgt cccaggcccg cggccaggat
     1741 cacggcggcc accggtgatc ggtccgtcat cggtcgcata ctggaccgat gatcgacgag
     1801 gcgctcgccg cggttcgcgg ctggcgggcg gacgggcgcg aggccgcgct cgcgacggtg
     1861 gtccgggtcg agggatcggc gccgcgcgag gttggcgcga agctggcggt ggcggccggt
     1921 ggcgagatgg ctggatcggt gagc
//
LOCUS       DASYGU010000232         6109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1116168, whole genome shotgun sequence.
ACCESSION   DASYGU010000232 DASYGU010000000
VERSION     DASYGU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116168"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGO32_06515"
     CDS             <1..294
                     /locus_tag="VGO32_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV7200437.1"
                     /translation="EFDIDPDLLHESSAGDVKRVTRMDRLITPIKLPSDPLAASYAVA
                     GVLQVELSRKQRLLEAPDAAARLRAELAILQREAQLLGTASLAPMGRFEYHAN"
     gene            479..1195
                     /locus_tag="VGO32_06520"
     CDS             479..1195
                     /locus_tag="VGO32_06520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV7200438.1"
                     /translation="MASGCTASNRVPIGEPEIIVRAPDAGEAMALTFDMGGRLDPALD
                     IMAFLVENEVCTTLFPTGAMSGTATGQAVLEIVAEHPELFELGNHTMHHCNLRDGGMG
                     SPTTAPCQTGGPPTQTFVRAELTDAAAILEAGSGQDPVPYWRPPFGAYTDDVLATAAG
                     LGYTKTIMWTMDTADWMPLEDGGPTAEQIATRVVTSAGSGSIILMHLGGYETLDALAI
                     MVPALRERGLMLTSVSDLLD"
     gene            complement(1192..1740)
                     /locus_tag="VGO32_06525"
     CDS             complement(1192..1740)
                     /locus_tag="VGO32_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200439.1"
                     /translation="MAHVVNLRRTYLCAVVLLFGAGCTTASGEPAPSNTPFSAAGQAA
                     AIGCGNDVDLFAPDGREVNLTGLWTATDFPDEPLWNIRQVGDCFFMVFRDPFEDSDYS
                     DEICDGHIRPDFEITGRCVDFRGAGGRGADLQPQVFLIQFDATNEVRLQRCPASRAPG
                     SCEEPMIRFDPDAGPTETASPT"
     gene            complement(1753..2160)
                     /locus_tag="VGO32_06530"
     CDS             complement(1753..2160)
                     /locus_tag="VGO32_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200440.1"
                     /translation="MLSQFLAVTTLVVTDLDLAKPFYEETLGLTLLEETPASCRFGAG
                     KGSQLTIRRGETPAGGQTVAHFEVEDIEAVVRDLTSRGVRFEEYESPKTVNGIAQFGP
                     SRGAWFKDPAGNVLGLRQGPVPTAPTAASAGSG"
     gene            complement(2196..2873)
                     /locus_tag="VGO32_06535"
     CDS             complement(2196..2873)
                     /locus_tag="VGO32_06535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV7200441.1"
                     /translation="MSATPDELMIAAAAAELVGVRTVFVGIGLPNAAANLARQTVAPD
                     LELIYESGVIGSRPTRLPDSIGDPALVSGATATMSMHDLFAYFLQGGRIEVGMLGAAQ
                     IDRWGNLNTTVIGPYEHPTTRLPGSGGACEIALNAARVVIVMNQTRRTFVEQVDFVTS
                     PGHRHPEGTPRPAWASAGPTSVITQLATYRFGADGEMELAAPHDGVSEADVDAASCWP
                     IRRASST"
     gene            complement(2870..3802)
                     /locus_tag="VGO32_06540"
     CDS             complement(2870..3802)
                     /locus_tag="VGO32_06540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV7200442.1"
                     /translation="MKPEAERTPGKVLSLAEAVAAHVHDGDVVAIEGFTHLISFAAGH
                     EIIRQGRRDLTLARLTPDLVYDQLVAAGCARKLVFSWLGNPGVGSLHAIRRAVETGSL
                     EIEEYSHFGMVGRYAAGAARLPFYPLRSYAGSDLPDANPLIQWVASPYAPADEIAVVP
                     PLNPDVTIIHAQRADAEGNTQVWGLVGVQREAALAAERVIVVVEELVDGDVVRSDPDR
                     TLLPGVAVTAVCVEPYGAHPSFAQGYYDRDNAFYRDWDPISRDPARLDAWLDEWVRGV
                     PDRAAYLAKLDHEVLDALRPGPRPSGSVDYGEYR"
     gene            complement(3786..5159)
                     /locus_tag="VGO32_06545"
     CDS             complement(3786..5159)
                     /locus_tag="VGO32_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEV7200443.1"
                     /translation="MTVRAFRQAADDAPAVPHLVTELPGPRAREIIARDDAVTSPSLT
                     RAYPLVAESGSGYVVTDVDGNQFLDFAAGIAVALTGHAHPAVSKAIREQSEKLIHIAA
                     TDFYEPRYLELCERLAAVAPFEEHARVFLTNSGTEAVEGAIKLARHHTGRPAIIAFEG
                     AFHGRTMGSLSLTNSKIKQRAGFGPLLPMVYHAPFPRVRAWHEGTGGDGTAELDHLRR
                     TILGRLVSPEDVAAIVVEPIQGEGGYFPAPPAFIHGLREICDEHGIVLIADEIQSGMG
                     RTGDMWAMEGYSVEPDVVTVAKGIASGMPLGAFIGKEKLFTWTAGTHGSTFAGNPVCC
                     AAALATLDLLEGGLMENAARMGERLRAGVEYASEECGVVRDIRGKGLMLGVEFGSHEE
                     ANAVQFAAFERGLLVLECGEASIRFCPPLIVDEAAVDTAIRLYAESLASVGCPIRPLP
                     GMAETGG"
     gene            complement(5206..>6109)
                     /locus_tag="VGO32_06550"
     CDS             complement(5206..>6109)
                     /locus_tag="VGO32_06550"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEV7200444.1"
                     /translation="AGAWSARPVEHMGLRFRNPVGLAAGFDKDGEAIAGWGALGFGFA
                     EVGTVTPVAQPGTARPRVWRLPDDGALVNRMGFNNHGAAALADRVARARPWLPRDFVV
                     GVSIGRGSGTGDEAAEGDYLAAARAVAPVADYLAINVSSPNTAGLAALEEPDRLAALV
                     VALDTVEPRPPLVVKLSPDQPLERLRGLCQALESTPAAGLIVSNTTRSRDGLRSHVPP
                     GADLGGLSGRPLLPGMLASIEAIRAASGDRFTIMASGGIFSGEDARRARKAGADLVQI
                     WTGMVYAGPGLIGEVVEALTSAAI"
BASE COUNT          915 a         2154 c         2155 g          885 t
ORIGIN      
        1 gagttcgaca tcgacccgga cctgctccac gaatcgtcgg ccggcgatgt gaagcgggtg
       61 acccggatgg atcggctcat cacccccatc aagcttccgt cggacccgct ggctgccagc
      121 tacgcggtcg cgggcgtcct ccaggtcgag ctgtcacgca agcagcgact gctcgaggca
      181 cccgatgcgg cggcccgtct ccgcgccgag ctggcaatcc tgcagcgcga agcccagctc
      241 ctgggcaccg cctccctcgc cccgatgggc cggttcgagt accacgccaa ctagaccgat
      301 cccgggcggc gcccgtcgtc cggccgcgtt cctcctcgcc gctgtcctgg tcgtagccgg
      361 ctgcgcctcg gcctcgccgc ccccctcggt caccgcgtcg gcggcaccat cggtctcacc
      421 cacgccagag gcgccaaccc cggaaccgac cccgaccttc acgcccggcc ccacgccggt
      481 ggccagcggc tgcaccgcct cgaaccgggt cccgatcggg gagccggaga tcatcgtccg
      541 tgcccccgat gcgggcgagg ccatggccct gaccttcgac atgggtgggc gtctggatcc
      601 ggcgctggac atcatggcct tcctggtcga gaacgaggtc tgcaccaccc tgttcccgac
      661 cggggcgatg agcgggaccg cgaccgggca ggcggtcctt gagatcgtgg ccgagcatcc
      721 cgagctgttc gagctcggca accacacgat gcaccactgc aacctgcgcg atggcgggat
      781 gggctcgccg acgaccgcgc cgtgccagac cggaggaccc ccgacccaga cgttcgtccg
      841 agccgagctg accgatgcgg ccgcgatcct cgaagctgga tcgggccagg acccggttcc
      901 gtactggcgg ccaccgttcg gcgcgtacac cgatgacgtg ctcgccacgg ccgccgggct
      961 gggctacacg aagacgatca tgtggaccat ggacaccgcc gactggatgc cgctggagga
     1021 cgggggaccg accgccgagc agatcgcgac tcgggtcgtg acctcggccg gcagcggctc
     1081 gatcatcctc atgcacctgg gcggctacga gacgctcgac gcgctcgcga tcatggttcc
     1141 cgcgctgcgt gaacggggcc tgatgctgac ctccgtcagc gacctgctgg actaggtcgg
     1201 cgaggcggtc tcggtaggac cggcgtcggg atcgaagcgg atcatcggct cctcgcagga
     1261 gcccggcgcg cggcttgccg ggcaccgctg gagccggacc tcgttcgtgg cgtcgaactg
     1321 gatcaggaac acctgcggct ggaggtccgc cccgcgccct ccggcgcctc ggaaatccac
     1381 gcagcgacct gtgatctcga agtcgggccg gatatggccg tcgcagatct cgtcggagta
     1441 gtccgaatcc tcgaaggggt cgcggaagac catgaagaag cagtccccga cctggcggat
     1501 gttccacagc ggttcgtccg ggaaatcggt cgcggtccac agtcccgtga ggttgacctc
     1561 gcggccgtcg ggggcaaata ggtcgacgtc gttcccgcag ccgattgccg ccgcctgacc
     1621 ggcggcggag aacggcgtgt tgctcggagc gggctcgccg gaggcggtgg tgcagccggc
     1681 gccgaacagg agcacaacgg cgcagagata ggttcgccgg aggttcacga cgtgggccac
     1741 cgacctgctg gactagccgc tgccagcgga ggcggccgtc ggggcggtgg gtaccggccc
     1801 ctggcgcagg ccgagcacgt tgccggccgg atccttgaac cacgcaccgc gagaaggccc
     1861 gaactgggcg atcccgttga cggtcttggg cgactcgtat tcctcgaacc tgaccccgcg
     1921 cgaggtcaga tcccggacga ccgcctcgat gtcttcgacc tcgaagtgcg cgaccgtctg
     1981 gccgccggcc ggggtctcac cccgccggat cgtcagctgc gaccccttgc cggcgccgaa
     2041 tcgacagctt gccggggtct cctcgagcag ggtcaggccc agcgtctcct cgtagaacgg
     2101 cttggccaaa tcgagatcgg tgaccaccaa cgtcgtgacc gccagaaatt gggagagcat
     2161 gattcctacc tctcttgact ggtgcctgag ggccatcagg tggacgaagc gcggcggatc
     2221 ggccagcacg aggcggcgtc aacatcggcc tcgctgacgc cgtcgtgggg cgccgccagc
     2281 tccatctcgc cgtcggcgcc gaatcggtag gtcgcgagct gggtgatgac cgatgtcggg
     2341 ccggcgctcg cccaggcggg gcggggcgtg ccttcgggat ggcggtggcc gggtgaggtc
     2401 acgaagtcga cttgctccac gaacgtccgt cgcgtctggt tcatgacgat cacgactctg
     2461 gccgcgttca gcgcgatctc gcacgcgccg ccggatccgg gcagccgcgt ggtggggtgc
     2521 tcgtatggcc cgatgaccgt cgtgttgagg ttgccccagc ggtcgatctg cgcggcgccg
     2581 agcatgccga cctcgatcct gcctccctgc aggaagtagg cgaacaggtc gtgcatggac
     2641 atcgtcgcgg tcgcgccgga aacgagggcc gggtctccga tgctgtcagg cagccgggtt
     2701 ggccgcgagc cgatgacgcc ggactcgtag atgagctcga ggtcgggagc cacggtctgg
     2761 cgggcgaggt tggcggcggc attgggcagg ccgatcccga cgaacacggt ccgcaccccg
     2821 accagctccg cggccgcggc cgcgatcatc agctcgtccg gcgtcgcgct catcggtatt
     2881 cgccgtagtc gaccgatccg gacgggcgag gtccgggccg gagcgcatcg agcacctcgt
     2941 gatcgagctt ggcgaggtat gccgctcgat cggggacgcc gcgaacccat tcgtcgagcc
     3001 acgcgtccag ccgcgccggg tcgcggctga tgggatccca gtcgcgatag aacgcgttgt
     3061 ctcggtcgta gtagccctgg gcgaacgacg ggtgggcccc atacggctcg acgcagacag
     3121 cggtgactgc cacaccgggg agcagcgttc ggtccgggtc ggagcggacg acgtcgccgt
     3181 ccaccagctc ctcgacgacc acgatcacgc gctccgccgc gagcgccgcc tcgcgctgga
     3241 ccccgaccag gccccagacc tgggtgttgc cctcggcgtc ggcccgctgg gcgtggatga
     3301 tcgtcacgtc ggggttgagc ggcggcacga ctgcgatttc gtcagcgggc gcgtacgggg
     3361 aggcgaccca ctggatgagc gggttggcgt ccggcaggtc cgagccggcg tacgagcgga
     3421 gcgggtagaa ggggaggcgt gccgccccgg ctgcgtatcg gcccaccatc ccgaagtggc
     3481 tgtactcctc gatctccagg ctgccggtct cgaccgcccg ccggatggcg tgcagcgagc
     3541 cgacgcccgg gttgcccagc cagctgaata cgagcttgcg ggcacagcct gccgccacca
     3601 actggtcata caccaggtcc ggcgtgagtc gggccagggt caggtcgcgg cggccctggc
     3661 ggatgatctc gtgcccggcc gcgaacgaga tcaggtgggt gaacccctcg atcgcgacga
     3721 cgtcaccgtc atgaacgtgc gcggccacgg cctccgccag cgagaggacc ttgccgggcg
     3781 tgcgctcagc ctccggtttc agccatcccc ggcagtgggc gaatggggca gcccaccgat
     3841 gccagcgact ccgcgtacag gcgaatggcg gtgtccaccg ccgcctcgtc gacgatcagc
     3901 ggggggcaga aacggatgga cgcctcgccg cactcgagga ccagcagacc gcgctcgaac
     3961 gccgcgaact ggaccgcgtt tgcctcctcg tggctcccga actcgacgcc cagcatcagc
     4021 cccttgccgc ggatgtcgcg gactacgccg cactcctcgg aggcgtactc gacgccggcc
     4081 cggagccgct cgcccatgcg ggcggcattc tccatcagcc cgccctcgag gaggtccagg
     4141 gtggcgagcg ccgcggcgca gcacaccggg ttgcccgcaa acgtgctgcc gtgggtgccg
     4201 gcggtccagg tgaatagctt ctccttgccg ataaacgcgc ccagcggcat gcccgaggcg
     4261 atccccttgg ccacggttac aacgtccggc tccacgctgt agccctccat ggcccacatg
     4321 tcgccggtgc ggcccatgcc cgattggatc tcgtccgcga tgagcacgat cccgtgctcg
     4381 tcgcagatct cgcgcaggcc gtggatgaag gctggcggcg ccgggaagta gccgccctcg
     4441 ccctggatag gctcgaccac gatggccgcc acgtcctccg gcgatacgag ccggccgagg
     4501 atcgtccggc gcaggtggtc cagctcggcg gtgccgtccc cgccggtgcc ctcgtgccag
     4561 gcccgcaccc gggggaacgg ggcgtggtag accatgggca ggagcggccc gaagcccgcc
     4621 cgctgcttga tcttcgagtt ggtcagcgac agcgagccca ttgtccggcc atggaatgcg
     4681 ccctcgaacg cgatgatcgc gggacgaccg gtgtgatggc gggcgagctt gatcgcgccc
     4741 tcgacggcct ccgtccccga gttggtgagg aagacgcgag cgtgctcctc gaacggcgca
     4801 actgcggcca gccgctcgca cagctcgagg tagcgcggct cgtagaagtc ggtcgccgcg
     4861 atgtggatga gcttctcgga ctgctccctg atcgccttgc tcacggccgg gtgggcgtgg
     4921 ccggtcaggg cgaccgcgat gccggccgcg aagtccagga actggttgcc gtcgacatcg
     4981 gtaacgacgt acccgctgcc cgactccgcg acgagcgggt aggcccgggt cagtgaggga
     5041 ctcgtgacgg cgtcgtcacg ggcgatgatc tcgcgggcgc gcggcccggg aagctcggtg
     5101 acgagatggg ggacagccgg cgcgtcgtcg gccgcttgac gaaaggcccg aacggtcatg
     5161 tgcggatacc tgatcgaaag cggcgcgtcc tgcccgcgcc cctggctaga ttgccgctga
     5221 tgttagcgcc tcgaccacct cgccgatcaa gcccggcccg gcgtacacca tgcccgtcca
     5281 gatctggacc aggtcggccc cggccttccg agcgcggcgc gcgtcctccc ccgagaagat
     5341 gccgcccgag gccatgatcg tgaatcgatc gcccgaagcg gcccggatgg cttcgatcga
     5401 cgccagcatg ccgggcagca gcgggcgacc ggagagaccg ccgaggtcgg cgccgggcgg
     5461 gacgtgagac cgcagcccgt cccgcgagcg cgtggtgttc gatacgatca ggccggccgc
     5521 cggcgtggac tccagcgcct ggcagaggcc gcggagtcgt tcgagcggct ggtccggtga
     5581 cagtttcacg accagtggcg gacgaggctc aaccgtgtcg agcgcgacga ccagcgccgc
     5641 aaggcggtcc ggctcctcga gcgcggccag gccggcagtg ttcggggagc tgacgttgat
     5701 cgcgaggtag tccgcgaccg gggccacagc ccgcgccgcg gcgaggtagt ccccttccgc
     5761 cgcctcgtcg ccggtccctg atcctcggcc gatcgacacg ccgaccacga aatcacgcgg
     5821 cagccacggt cgggctcgcg ccacgcgatc ggccagcgcg gcggcgccgt ggttgttgaa
     5881 ccccatccgg ttgaccagcg ctccgtcgtc gggcaggcgc cagacccggg gccgcgcagt
     5941 gccgggctgc gcgacggggg tcacggtccc gacctcggcg aagccgaagc caagggcgcc
     6001 ccagccggcg atcgcctccc cgtccttgtc gaagccggcg gccagcccca ccgggtttcg
     6061 gaagcggagg cccatgtgct ccaccggccg cgccgaccag gcgccggcc
//
LOCUS       DASYGU010000233         6470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1322477, whole genome shotgun sequence.
ACCESSION   DASYGU010000233 DASYGU010000000
VERSION     DASYGU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322477"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1497)
                     /locus_tag="VGO32_06555"
     CDS             complement(133..1497)
                     /locus_tag="VGO32_06555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEV7200445.1"
                     /translation="MPAASLDVLASLAKRRGFVFGGSEIYGGLQGFWDYGPLGVELRT
                     NIKAAWWRSMVRLRDDIEGLDAAIIMNPRVWEASGHVAGFSDPMVDCRNCNHRFRESD
                     LKGPPSEIACPDCGQRGTLTEARQFNLMFKTHVGPMEEGASVAYLRPETAQGIFVNFA
                     NVATAMRRKPPFGIAQIGKSFRNEITPGNFIFRLREFEQMEMEFFIPPADEDRWFGYW
                     VEERLRWWTDEIGINAERLRLRPHDADELSHYSRQTTDIEYLFPMGWSELEGVADRTN
                     FDLSAHGAASGKNLTVFDEATGDHIVPFVIEPAMGVDRATLTVLVDGYDEEEVGGEKR
                     VVLRIRPSLAPVKVAVLPLLRNRPELVALGRKLTTDLKRDVAAMYDDTASIGKLYRRQ
                     DEIGTPFCVTVDVETLTDGAATIRERDTMTQERVALDAIPARIRELVAGGPWTGSAPA
                     PVSE"
     gene            complement(1509..2330)
                     /gene="recO"
                     /locus_tag="VGO32_06560"
     CDS             complement(1509..2330)
                     /gene="recO"
                     /locus_tag="VGO32_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV7200446.1"
                     /translation="MSPRSRLYKTEAIVLRGLDLGEADRVLTVLTPHLGKLRVIAKGI
                     RRPRSRIGGGLEPLGQVDLVLAVGRTFDVVTQSQLVDPHLGMRTDLHATATAWYIAEL
                     ADRFCEERAESAAAFSLLGTGLTALDGVLTVATRDAIARAFELHLLEAMGYRPQLGAC
                     IECGAEIGPGQNRYAAVAGGVVGPECVHEALGAVAISTPALKAMRHLQRSPLPEAALL
                     DLSAPVGRELERHLHATVSAVLERELRSRDFLDEVGRLDHAGRHRPAAGATSVLR"
     gene            2358..3398
                     /locus_tag="VGO32_06565"
     CDS             2358..3398
                     /locus_tag="VGO32_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7200447.1"
                     /translation="MDIRVEFSQMVADIEAEMQSILDEHRGPAAPMYDMLAYHLGLDG
                     TDADGGKRIRPLLGLLVIRALGGDYHRALAGAAAVELGHNFSLVHDDIQDGDLERRHR
                     ATLWALWGVPQAINAGDALFALSRLALFRLSEAEDDPDAPDARQILDLMKIYDQTCLA
                     LCEGQFLDISFEARLDVTVEEYLQMIELKTASLMAAGVEAAATLTSKDPAVVTRLRRF
                     GDRLGLAFQMADDVKGAFWDESASGKAEAGDVRRRKKTMPVIWALNHASEADQARLRE
                     LFTPGADGAQLTDAEVVEGLAILDRSGARETTLGEARRYRDEALAELETLDLEPGPRA
                     ELELFVEAVIAA"
     gene            complement(3395..4777)
                     /locus_tag="VGO32_06570"
     CDS             complement(3395..4777)
                     /locus_tag="VGO32_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV7200448.1"
                     /translation="MPEGAAGTGNPAIDLLIVFILILIGGVFAAAEIALLTVRRTRLE
                     QLADEGKRGAETARRLVEDPQRFLATIQVVITFLGFLASAIGAIAFSAVIADAISTIP
                     LEPVQDAAETIAFILVTLLIALTSIIAGELVPKTLALNFPERFSLVAARPLSVMERVL
                     APLVWLTTTISGFLVRLFGGRERPQTGLLSVEELKLLVETGSAQGQIEEAEKEMIHGV
                     IELGDKQIHEVMIPRIGIKAVEVDDPIEEVLDLIVRAGHSRVPVYRENLDNIVGILYA
                     KDMLPYLLPAVEEREPLEIRSLARPAAYVPETKRVDEMLTEMRAARRHIAIVVDEYGG
                     TAGLVTIEDLVEEIVGEIQDEYDMEESLVEPVETDGDRRAFRLDGRVTMDDLRDLFEL
                     GDAEEPDEESFDTIGGLIIHRVGRIPLVGTEVDFRDLTLRVLAADSRRVSKVLAVQVE
                     ASRETAENNR"
     gene            complement(4770..6140)
                     /gene="glmU"
                     /locus_tag="VGO32_06575"
     CDS             complement(4770..6140)
                     /gene="glmU"
                     /locus_tag="VGO32_06575"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV7200449.1"
                     /translation="MSSLAAIVLAAGQGTRMRSRLPKVLHPLAGRPMIQHVLLSLDAV
                     AAAPVVVVTGHGADAVEAALAESVVAIRQEPQLGTADAVRVALSSVPDTIDQVLVTMG
                     DVPLLPTELLTALVHEQSASGAPFVLLSAHLPDATGYGRIVRGPDGACTAIVEEADAD
                     AATRALDEVNTGTYCFAAAWLRATIERVPLSAAGEHYLTDLVALATAEGQAVRVVTAR
                     QPELATGINDRVQLAAADRLLRARIAEEHMRAGVTIVDPASTFIDASVVIGQDARIEP
                     WTVLTGSTVIGEEAVVGPGSRIRDSRIDARAHVYGSWIEEAEVGEGAQVGPMSHLRPG
                     SVVGPGSEIGNYAEIKKSRLGARVRQHHFSYLGDAQVGDDVNIGAGTVTANFDGTDKH
                     ETIIGDGAFIGVDTMLRAPVTIGPGAKTGAGSVVTRDVAAGKTVVGMPARPIETRRRG
                     GEPPDA"
     gene            complement(6218..>6470)
                     /locus_tag="VGO32_06580"
     CDS             complement(6218..>6470)
                     /locus_tag="VGO32_06580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV7200450.1"
                     /translation="GGVATEAPQALRNVSAILDAAGSSMDRVLKTTVFLVDMADYPTM
                     NEAYRAAFPEPFPARTTVVVTALPAGANVEIEAVAVAGD"
BASE COUNT          981 a         2256 c         2229 g         1004 t
ORIGIN      
        1 cgcgcgcccg cagctcgtgc gccgcgtcct gcccgggatt ggcgtccagg atggtgctgg
       61 ccagccggat cacggtccgg tggtcgccgc ggtccgccgc cgccgacagg tcggcgaaag
      121 cggctccgcc ggtcattcgg aaaccggcgc cggggcgctc cccgtccacg ggccgccggc
      181 gacgagctca cggatccgag cggggatcgc atccagcgcg acccgctcct gggtcatcgt
      241 gtcgcgttcg cggatggtgg cggcgccgtc cgtcagcgtc tccacgtcca ccgtcacgca
      301 gaaaggagtc ccgatctcat cctggcggcg atagagcttg ccgatggacg cggtgtcgtc
      361 gtacatggcg gccacgtccc gcttcaggtc cgtggtcagc ttccgaccca gcgccacgag
      421 ctcgggtcgg ttgcggagca ggggcaggac ggcgaccttg accggcgcga gcgacggccg
      481 gatccgaagg acgacgcgct tctcgccgcc cacctcctcc tcgtcgtagc cgtccacgag
      541 cacggtcaag gtggctcgat cgacccccat tgccggctcg atcacgaagg ggacgatgtg
      601 atcgcccgtc gcctcgtcga acacggtcag gttcttgccc gaggccgcgc catgcgcgct
      661 gaggtcgaag ttggtccgat ccgccacccc ctccagctcg gaccagccca tcgggaagag
      721 gtactcgata tccgtggtct ggcggctgta gtggctcagc tcgtcggcat cgtgcgggcg
      781 gagccggagg cgctcggcgt tgatcccgat ctcatcggtc caccaccgga gccgctcctc
      841 gacccagtag ccgaaccatc gatcctcatc ggccggcggg atgaagaact ccatctccat
      901 ctgctcgaac tcgcggagac ggaagatgaa gttgccgggc gtgatctcgt tccggaagct
      961 cttgccgatc tgggcgatcc cgaacggcgg cttgcggcgc atcgcggtcg ccacgttcgc
     1021 gaagttcacg aagatgccct gggccgtctc cggccgcagg taggcgacgc tggcgccctc
     1081 ctccatcggc ccgacatggg tcttgaacat gaggttgaac tggcgggcct cggtcagcgt
     1141 ccctcgctgg ccgcagtcgg gacaggcgat ctcggacggg ggtcccttga ggtcgctctc
     1201 gcggaaccgg tggttgcaat tccggcagtc gaccatggga tcgctgaacc cggccacgtg
     1261 gcccgaggcc tcccacaccc gcggattcat gatgatcgcg gcgtccaggc cctcgatgtc
     1321 atcccggaga cggaccatgg atcgccacca ggcggccttg atgttcgtcc gcagctccac
     1381 gcccagcggc ccgtagtccc agaagccctg gaggccgcca tagatctcgc tcccgccgaa
     1441 cacgaagcca cgccgcttgg cgaggctggc gaggacgtcg aggctggcag cgggcacggc
     1501 aggcgagtct agcgcaggac cgatgtggcc ccggccgcgg gccggtgccg gccggcgtgg
     1561 tccagccgtc ccacctcgtc gaggaagtcg cggcttcgca gctcgcgctc gaggaccgcg
     1621 gacacggtgg cgtggagatg gcgctcgagc tcgcggccga ccggcgcgct gagatccagg
     1681 agcgcggcct cgggcagcgg cgagcgctgg aggtggcgca tggccttcag cgcgggcgtg
     1741 gagatggcga ccgcgccgag ggcttcgtgc acgcattccg gcccgaccac gccgccggcg
     1801 accgccgcgt atcggttctg gcccggcccg atctccgccc cacactcaat gcaggctccc
     1861 agctgcggcc ggtagcccat cgcctcgagc agatgcagct cgaacgcgcg ggcaatggcg
     1921 tcgcgggtcg cgaccgtgag cacgccatca agcgcggtca gccccgtccc gagcaggctg
     1981 aacgccgcgg cgctctcggc ccgctcctcg cagaagcgat ccgcgagctc ggcgatgtac
     2041 cacgcggtgg cggtggcgtg gaggtctgtc cgcatgccga ggtgcgggtc caccagctgc
     2101 gactgggtca cgacgtcgaa cgtgcgcccg accgcgagga cgaggtccac ctggccaagg
     2161 ggctccagtc cgccgccgat ccgggaacgc ggccgtcgaa tgcccttggc aatgactcgc
     2221 agcttgccga ggtgcggggt gaggacggtc aggacccgat cggcctcgcc caggtccagg
     2281 ccacgaagca cgatggcctc ggtcttgtac aggcgagagc gcgggctcac cggtagccgg
     2341 tcgggtaggc ttcgggcgtg gacatccggg tcgagtttag ccagatggtt gcggacatcg
     2401 aagccgagat gcaatccatc ctcgatgagc atcgcggccc ggcggcgccg atgtacgaca
     2461 tgctcgccta ccacctgggc ctggacggga ccgatgccga cggtgggaag cgaatccggc
     2521 ccctcctcgg tctgctcgtc attcgggctc tcggcggcga ctatcaccgc gcccttgccg
     2581 gcgccgccgc cgtcgagctg ggccacaact tcagcctggt ccatgacgac atccaggacg
     2641 gcgatctcga gcgccgccat cgcgccaccc tctgggcgct gtggggcgtc ccacaggcga
     2701 tcaacgccgg cgacgcgctg ttcgccctct cgcggcttgc cctgttccgc ctgtcagagg
     2761 ccgaggacga tcccgacgca cccgacgccc gccagatcct cgacctcatg aagatctatg
     2821 accagacctg tctcgcgttg tgcgagggcc agttcctgga catcagcttc gaagctcgac
     2881 tggacgtgac cgtggaggag tacctccaga tgatcgagct caagaccgcc tcgctgatgg
     2941 ccgccggcgt cgaagcggcc gcgaccctga cctcgaagga cccggccgtg gtcacccgtc
     3001 tccggcgctt cggggatcgg ctggggctcg cgttccagat ggctgatgac gtgaagggcg
     3061 ccttctggga cgagtccgcg tccggcaagg ccgaggcagg cgacgtccgg cggcggaaga
     3121 agacgatgcc cgtgatctgg gccttgaacc acgcctcgga agccgaccag gcccggctgc
     3181 gcgagctctt cacaccgggc gccgatggcg cgcagctgac cgatgccgag gtggtcgagg
     3241 gcctcgccat cctggaccga tcgggagcgc gcgagacgac cctgggcgag gcgcgccgat
     3301 accgcgatga agcgctggca gagctcgaga cgctcgatct cgaacccggg ccccgggccg
     3361 agctcgagct gttcgtggag gcggtgatcg ccgcctagcg gttgttctcg gccgtctcgc
     3421 ggctggcctc gacctggacg gcgaggacct tggacacccg ccgcgagtca gcggccagca
     3481 cgcggagggt caggtcacgg aagtcgacct cggtgcccac cagcgggatg cggccgaccc
     3541 ggtggatgat cagcccgccg atcgtgtcga acgactcctc gtcgggctct tccgcatcgc
     3601 cgagctcgaa caggtcgcgg aggtcgtcca tcgtcacccg gccgtcaagc cggaaggcgc
     3661 ggcggtcgcc atcggtctcg accggctcga ccaggctttc ctccatgtcg tactcgtcct
     3721 gtatctcgcc cacgatctcc tcgacgaggt cctcgatggt gaccagcccc gccgtcccgc
     3781 catactcgtc gaccacgatc gcgatgtggc gccgggccgc gcgcatctca gtcaacatct
     3841 cgtccacccg cttcgtttcg ggcacgtacg ccgccggccg ggccagggag cggatctcga
     3901 gcggctcgcg ctcctcgacc gcgggcagca ggtacggcag catgtccttg gcgtagagga
     3961 tcccgacgat gttgtcgagg ttctcgcggt agaccggaac ccgggagtgg ccggcgcgga
     4021 cgatgaggtc cagtacctcc tcgatcggat cgtcgacctc gaccgccttg atcccgatcc
     4081 tgggaatcat gacctcgtgg atctgcttgt cgccgagctc gatgaccccg tggatcatct
     4141 ccttctcggc ctcttcgatt tgaccttgcg cggatcccgt ctccaccagc agcttcagct
     4201 cctccacgga cagcagcccg gtctgcggtc gctcgcggcc cccgaacagg cgaaccagga
     4261 agccgctgat cgtggtcgtg agccacacga gcggggccag gacgcgctcc atgacgctga
     4321 gcgggcgcgc ggcgaccaac gagaaccgct ccgggaagtt cagggccagg gtcttcggca
     4381 ccagctcgcc ggcgatgatc gaggtcagcg cgatgagcag cgtgaccagg atgaacgcga
     4441 tcgtctcggc ggcatcctgc acgggctcca gcgggatggt cgagatcgcg tcggcaatga
     4501 ccgcactgaa cgcgatcgcg ccgatggccg acgcgaggaa gccaaggaac gtgatcacga
     4561 cctggatcgt tgccaggaag cgttgcgggt cctccaccag ccggcgcgcc gtctccgcgc
     4621 cgcgcttgcc ttcgtcagcg agctgctcga gacgcgtgcg gcgcacggtc aggagcgcga
     4681 tctcggccgc cgcgaatacg ccgccgatga ggatcaggat gaatacgatc aggaggtcga
     4741 ttgccggatt gcccgtcccg gccgcgcctt caggcatccg gaggctcacc tccccggcgt
     4801 cgggtttcga tcgggcgggc gggcatgccg accacggtct tgcccgcggc aacgtcacgg
     4861 gtcaccacgc tgccggctcc ggtcttcgcg ccgggcccga tggtcaccgg cgcccggagc
     4921 atggtgtcga ccccgatgaa cgccccgtct ccgatgatcg tctcgtgctt gtcggtgccg
     4981 tcgaagttgg cggtcaccgt gccggcaccg atgttcacgt cgtcgccgac ctgcgcgtcc
     5041 ccgagataac tgaagtggtg ctgccgaacc cgggctccca ggcggctctt cttgatctcg
     5101 gcgtagttcc cgatctcgga tccaggcccg accacactgc ccggtcggag gtggctcatc
     5161 ggaccgacct gggcaccttc ccccacctcg gcttcctcga tccacgaccc gtagacgtgg
     5221 gcacgcgcgt ctatccggct gtcgcgaatt cggctgccgg gcccgaccac cgcctcctcg
     5281 ccgatgaccg tgctcccggt gaggaccgtc cagggctcga tccgggcatc ctggccgatg
     5341 accactgagg cgtcgatgaa cgtgctggcc gggtcgacga tggtgacgcc ggcccgcatg
     5401 tgctcttcgg cgatccgcgc gcggaggagc cgatcggccg ccgccagctg gacgcggtcg
     5461 ttgataccgg tcgccagctc gggctgccgc gcggtgacga cccggaccgc ctggccctcg
     5521 gccgtggcca gcgcgacgag atcggtgagg tagtgctcgc ccgccgcgct gagcggcacg
     5581 cgttcgatgg tggcccgcag ccacgccgcc gcaaagcaat acgtgcccgt gttcacctcg
     5641 tccagggcgc gggtggcggc gtcggcatcg gcctcctcga cgatcgcggt gcacgcaccg
     5701 tccggcccgc ggacgatgcg tccgtagccg gtggcatcgg ggagatgcgc tgagagaagg
     5761 acgaacgggg caccactggc cgattgctcg tgcacgagcg cggtcaggag ctcggtcgga
     5821 aggagcggga cgtcgcccat ggtcacgagg acctgatcga tggtgtcggg cacgctcgat
     5881 agcgcgaccc ggaccgcatc cgcggtgccc agctggggct cctggcggat cgcgaccacg
     5941 gattcggcca gcgcggcttc gaccgcatcg gcgccatgcc ccgtgacgac cacgaccggt
     6001 gccgcggcga ccgcgtcaag gctcagaagg acgtgctgga tcatcggtcg accggcaagt
     6061 ggatggagca ccttcggaag ccgagaccgc atccgggttc cctggccggc ggccagcacg
     6121 atcgcagcga ggctgctcac gccagggggc gcttggaagg gcgcatcaag aggtgtggat
     6181 ggtaggaggg cgttcgaacg agcgtcaact tgcccgacta gtctcccgcc accgcgacgg
     6241 cctcgatctc gacgttggcg ccggccggga gggcggtgac cacgaccgtc gtccgcgcag
     6301 gaaagggttc ggggaacgcc gcacggtacg cctcgttcat ggtcgggtag tcggccatgt
     6361 cgaccaggaa gacggtcgtc ttgaggaccc gatccatgct cgatccggcg gcatcaagga
     6421 tcgcggagac attccggagc gcctgaggcg cctccgtcgc gacgccgcct
//
LOCUS       DASYGU010000234        15237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1683031, whole genome shotgun sequence.
ACCESSION   DASYGU010000234 DASYGU010000000
VERSION     DASYGU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683031"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VGO32_06585"
     CDS             <1..864
                     /locus_tag="VGO32_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV7200451.1"
                     /translation="AIFRERRRPLPAAALATAAMNGRRTPPGLQPVPMVADRGVNGNG
                     HNGSDGNGSDGGAYPPLSELAAASAIGETNGFAYFVAAEGHNGENGNGSSGAWAGDYV
                     DRLHALLAALDLHAVDHMADELLAARLRGATIFIAGNGGSSATAAHWVNDLAKATRRA
                     DQPPMRVMNLTDNVPWLTALANDEGYDRVFAGQLENLAREGDVLVVISASGNSPNILR
                     AIELANASGLVTMGLLGFDGGAALSLLDTAVWLPTRHGEYGLVETAHSAVADMVTARL
                     AVRPVSAPTSG"
     gene            871..2088
                     /locus_tag="VGO32_06590"
     CDS             871..2088
                     /locus_tag="VGO32_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEV7200452.1"
                     /translation="MPTQRPRQAVILAGGRGTRMRPLTDDRPKPMIEFSGRPFMEYMV
                     ELLAEQGFERVLMLLGYLPDVIRDHFGDGSRFGIRIEYSVTDPDDLTGRRLQVADDLL
                     DPLFMLIYCDNYWPLNMERMWDHYQRVGAPAMVTIYANQDGYSKGNTLVGADGLVQAY
                     DRSRTSENLTGVEISYAIFPKRFLALLPADGAELVEEAVYPRLTADRLLGAHVSEHRY
                     YSVGSMHRMPLTEAFFARRPTVILDRDGVLNERPPRAEYVRTPADFQWLDGSLEALRL
                     FTDAGYQVVVISNQAGIGRGVMTESDLTAVHDRMVRQAADAGGRIDAIYHCPHDWDAG
                     CTCRKPSPGMLFEAQHDLQLDLSRTPFIGDDERDGQAAYAAGAPYLAVDDSTSLLAHA
                     RRLTASATAAVHA"
     gene            2085..3137
                     /locus_tag="VGO32_06595"
     CDS             2085..3137
                     /locus_tag="VGO32_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7200453.1"
                     /translation="MSAQRRVLVTGHRGYVGAVMAPAFREAGFDVVGMDVDFFRDCDL
                     FPLADIPSIEKDIRDVEASDLEGFDAVVHLAALSNDPIGNLNEAWTADINVDGTVRLA
                     EAAREAGVGRFLFSSSCIMYGMAEAGAVDEDSPLNPQTEYARSKVVAERALSARATPS
                     FSPVYLRNGTIFGVSPRMRFDTVLNNLTGMAVTTGAITVMSDGTPWRPLVHVRDVARA
                     FIAVVDAPVEAVHDQAFNLGADALNHQVRDLAHAVQRAVPEASLEIRAVPDADQRTYR
                     TSFAKFARTFPTFRFERNADDGAVELTAAFRDLGLTYEAFTSDRFTRLKRLGRLIEDG
                     SLDDALRWRIDEGMVA"
     gene            3137..4141
                     /locus_tag="VGO32_06600"
     CDS             3137..4141
                     /locus_tag="VGO32_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEV7200454.1"
                     /translation="MSGRGIVLTRTPLRVSFAGGGTDLATHYEADDGAVFSSTIDKYV
                     YVTVKRHGAVFDENVRLNYSVSESVSSIDAIRNDVARECLRLLEIEPPIYVSVVSDLP
                     DSSGLGGSSAFAVGLLNALHMFRGEQVTASQLADEASTVEIEMLAQPIGKQDQYAAAF
                     GGLNLFAFRPGGDVTVQPHRGVNGSVGELFDHLLMIWSGHQRRASSVLLEQQERTAEH
                     RGVLGRMREQAYELQALMSSPTPDFRRFGAVLDAGWNMKRGLASTVSNPEIDGWYAAG
                     LEAGALGGKLCGAGGGGFLVFLAEPGARDQVRAALPGLQSVPVRFEAHGSRVLFAEVD
                     "
     gene            4141..5082
                     /locus_tag="VGO32_06605"
     CDS             4141..5082
                     /locus_tag="VGO32_06605"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV7200455.1"
                     /translation="MAPIEPRPDFFIVGAPRCGTTAMYEYLRRHPEIFMPEHKEPMYF
                     GADLASIHGRLTEADYLRLFRPATTGQRIGEASTWYLYSTDAPREISAFSPEAQIIIM
                     LRNPVDVMYSLHGEVVFYGGEEIEDFEEALAAEPDRREGRRLGPTRRPEALFYRDTVR
                     FADQVERYLDEFGSERVQILLFDDFVRDPAAAYLEVLRFLGVDETFRPSFERVNESKR
                     PVNSTFQSFVVRPPGPVARLIPLLRRAPLAHQLRAAVLSANSRSYERPPLAEPLRQRL
                     TAELAPEVERLGELIGRDLSRWMPVATPGPKEARSVA"
     gene            5079..6446
                     /locus_tag="VGO32_06610"
     CDS             5079..6446
                     /locus_tag="VGO32_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200456.1"
                     /translation="MSRPQVASRRMTLSLPAALAPFRWTIGATSGLFAATLLTSATGF
                     VFWWIAAQGYSESAIGLAGAAVSAMLFLSQVAMLGYGTALAGILHREERPASLAVTAT
                     LAAGIAGLILGVGFAVLAPLLSSELQPLAAPLAIVIFALGVCVAALTSVLDQVLVAVN
                     QRLMQLIRNVIFGVGRLPVLLAIAAVVLWPDGMGIYVAWVVGLMLSLAVIVVLVHNAQ
                     LTGDIRPLMWGRLRAMAPDALAHHVVNLSRSGSVWLLPLLVTIVLSSEENAAFYIAFL
                     VSNLILLLGKEATFTLYIVGARAPETLWRQIRFTLGFCALASVLGTIVLSVLGPRVLG
                     VFGSSYETAYPVLLILSISALPVLVKDHWIAVHRVRGSVSKAAVVGVITLAMELAGAV
                     VGALWGEQLGFALLGFAIGRLVALLVQAAFMLPMLISVMRPPAGGTPVTPSATDADPT
                     EAVFA"
     gene            6443..7423
                     /locus_tag="VGO32_06615"
     CDS             6443..7423
                     /locus_tag="VGO32_06615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200457.1"
                     /translation="MSSSPIAISVIVCAFADDRWDDLVGALNSIQRQGLNAAETIVVT
                     DHNDGLGARVAAEFPWARVVPNSGQQGLSDARNTGVAASSAPIVAFLDDDAVASPGWL
                     EALADPYRDQRVIGVGGAAIPAWDARRPAWFPDEFDWVVGCSYRGLPTHLAPIRNPLG
                     CNMSFRREAFAATGGFDTSVGRVGRVPVGGEETEFCIRLRQQMPNHVLLYQPTATVRH
                     RVRAERATWRYFKARCVAEGRSKARITTLVGAGDGLASERRHAAVTLPRGVARHLSVA
                     LRELQPAGVVRAGAIVAGLGCTVAGYAAGRMGARQSRTPPKPEPTVGPAG"
     gene            7477..8592
                     /locus_tag="VGO32_06620"
     CDS             7477..8592
                     /locus_tag="VGO32_06620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7200458.1"
                     /translation="MVAHRFPPYTGGVETHTLEVARRVAADSRFEVHVLTTDLDRKLP
                     PREVVDGVQVTRVRVGPPGTDLYIGPDIFRRVRSTSADLVHCQGYHTFVPPLAMAAAQ
                     RARIPYVVTLHSGGHSSRVRRAIRPVQLRALRGQLAGAERLIAVSRFEAHEFARRLNV
                     PEDRFLVIPNGSDMPAVANAKLGPRDAGLILSVGRLERYKGHHRLIDALPRVRRERPD
                     ARLVILGSGPYLSELRHRAVERGVGDAVEIRSASRPEVAGLMAQAQVVALLSDYESQG
                     LAVHEALALGCRIMVSRSSALAEVADLPQARGVAPSAGSDEIADGLLAQLAAPSSIAD
                     PPILPSWDECAARVMDVYRAVLSGEDSGSGPIGSTTS"
     gene            complement(8529..9611)
                     /locus_tag="VGO32_06625"
     CDS             complement(8529..9611)
                     /locus_tag="VGO32_06625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200459.1"
                     /translation="MARFSIFIPIYNDGRWLAGAIESVLAQSHAEWELVVGDNASTEP
                     IEPIVRRFGDPRIRYHRWQTHARIDENFNRTALLTREPWVQFLGADDRLHPGCLARMA
                     TVIDGWAPDGDRLAMVVTACRRVDEQGRSADHIWYGTRPRWQVAAGVYDAAGWFDIHL
                     QDGNPPWNVGSVAVARDVLDESGGFFRPETGLSADVELSLRAAAYGSVAYIDEPLLDF
                     MVRPDADNSFRLWVNRSENDQRTPIEVALLAGLRTHQHRRAVSNAEASRLRAIIARSH
                     LQRAAQHRVLEGGRGRPGSARDIAAALRWSPRAVLSPYHAMYALATLVAPRRLLETAK
                     RRLSPAQEVVDPIGPLPESSPESTAR"
     gene            complement(9614..10909)
                     /locus_tag="VGO32_06630"
     CDS             complement(9614..10909)
                     /locus_tag="VGO32_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7200460.1"
                     /translation="MIIVDRALAERAASGRPVQVGMVGAGAMGRGIARQMFRAFPGLR
                     LSAIANRTVDSAVRAYHEAGQTEVDHAATVEDVEAAIRANRFAVTDDPFLVTEAPSIE
                     AVVEVTGTVEHAARVVESAIANGKHVILMNAELDGTVGPILKVHADEAGVILTACDGD
                     QPGVEANLYRFVQGIGLRPLVCGNIKGLQDPYRTPTTQEGWAQTWGLGVRMVTSFADG
                     TKVSFEQAIVANGTGFRAPRRGLYAWDHTGHVDELTTRYDVEELTRLGGIVDYVVGAQ
                     PAPGVYVMATHDDPLEQHYMKLYKRGDGPLYSFYTPQHLCHFEVPTSVARAVLLNDPV
                     LAPQGGPVVEVVATAKRDLEAGEVLDGIGGYTAYGQCDNADVVAGEGLLPMGIAEGCR
                     LTRAIPRDAVIGYADVELPTGRRVDKLRTEQGERFAAAG"
     gene            complement(10906..11451)
                     /locus_tag="VGO32_06635"
     CDS             complement(10906..11451)
                     /locus_tag="VGO32_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV7200461.1"
                     /translation="MRFTTTPLSGVMVLELEPREDERGFFARAFDVNEFKEHGLNPRI
                     VQANLSHNLRRGTVRGLHYQRPPFAEAKHIRCIRGGAFMAVVDLRPESATHLRHTTVE
                     LHEGDQRSLYVAEGCAVGLQALEDVTELLYNVSEFYTPEAEGGLRYDDPAFGIEWPLP
                     MTTISEKDAAWPHLGLTPARP"
     gene            complement(11512..12228)
                     /locus_tag="VGO32_06640"
     CDS             complement(11512..12228)
                     /locus_tag="VGO32_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200462.1"
                     /translation="MLSRPLSNQPRRHRWALAGGLALTIQLLVVSAALGASVPTDPDP
                     DLECPDAFGSGLVTQYVTTVNTPADSKAWWVSVEFTIADEVETPASCEITLASYELPG
                     PDFSFPQTLFDSDTGWFGAGTHTLTVDLPRDGAAAGCFAQYDFVFGPAIETLVFEDRY
                     GDRQIRSRIEGSETCPQDPVEPQTPPESGTEGGTGLPDTAMAPASSSDLGLLFAFLLM
                     ASLGTIGVLNVQAVRARRTR"
     gene            complement(12503..13744)
                     /locus_tag="VGO32_06645"
     CDS             complement(12503..13744)
                     /locus_tag="VGO32_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEV7200463.1"
                     /translation="MALHLVRFRLPLVGLALALMFTASPGTAVAAVPGVTGFTLINAD
                     TDQPIAGFNPIQPGATLDLASLPTRRLNVRVNTAAAVGSVRVTVNGGGARVENGQPYS
                     MTGDEAGDYRAWTPAVGSYTVTATAFAQRRAQGPANPPVSLTFRVVNTGSVPTTVPPP
                     PDTDDPSPSTCGPMPATAPAGWQRVVSDTFSENIPLGQWGRDGGAWEQPGGHWRARPD
                     GWKDSSRRGTYSSPKTTSQHDSLMDIWVHSEGVTRYVAAPISLVGDTLGQRISICMRA
                     DVIPGYKLAFLLWPSQGSGNERGEIDFPEGKLNGGSATARAFMHYDPRPSSGREQDAY
                     DTGVALQGWHTYTVAWRPQSGTTEFFVDGRLFGRSTQYVPRGPMHYIMQVETYMAGQV
                     LPPPASGHVLVDWVTIEVPRP"
     gene            14042..>15237
                     /locus_tag="VGO32_06650"
     CDS             14042..>15237
                     /locus_tag="VGO32_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEV7200464.1"
                     /translation="MPIATAFLILFAGVLLVGDKGPILTGGGPSHSPAPSVLSPSVSP
                     AAGSNQPSPSGDPSLPFGPGDPSLDGSVWYPPAIPVGAYATVAAVNSSAQNRDRADFV
                     VQGTADDEINAALADLARIGGGQVQLLEGLYELAAPITISGDGLALVGVNVGNAAGYS
                     EAALGSQIVPAAGFPDDAFLVNATAEAYGPLISLIHVDGLTRAQGMNIEGSRPTVSLN
                     AVSQSSGVGIRFAGESTGQRPYDGFVLFNRVFEGLGIGILHDQRSGDMLIEGNVVFRN
                     GGDGFLSRGASQMYSLNHAYNNAGVGIRLVPGCVRTRLTSNKWEGNDLGGLSIEGGSG
                     FTVIGDTFADNDSPSGTSAAHIELGVRGPTETIGVMLSNLAFGKGDDGNPYLIHFGSL
                     ARDVHVG"
BASE COUNT         2307 a         5147 c         5221 g         2562 t
ORIGIN      
        1 gcgatcttcc gagaacgacg acgaccgctg ccggcagccg cgctggcgac ggcagcaatg
       61 aacgggcggc gcacaccacc cggtcttcag ccggtgccga tggtggccga ccgcggcgtc
      121 aacggaaacg gtcacaacgg aagcgatggc aacgggagcg atggcggcgc ttatccgccg
      181 ctgagcgagc tggccgccgc ctcggcgatc ggcgagacga atgggttcgc gtatttcgtc
      241 gcggcagagg gtcacaacgg cgagaacggg aatggctcgt ccggcgcatg ggccggtgac
      301 tacgtggatc gcctgcatgc cctcctcgcc gcgctggatc tgcacgccgt cgatcacatg
      361 gcggacgagc tgctggctgc ccgcctccga ggagccacga tcttcatcgc cggcaacggt
      421 ggaagctcgg caaccgcggc ccattgggtg aacgacctgg ccaaagccac ccgtcgcgcg
      481 gaccagcccc cgatgcgggt catgaacctg accgacaacg tgccatggtt gaccgccttg
      541 gccaatgacg aagggtatga ccgggtcttc gccggtcagc tcgagaatct ggcccgggaa
      601 ggcgacgtcc tggtcgtcat ctcggcgagc ggcaactcac cgaacatcct tcgagccatc
      661 gagctggcga atgcctcggg cctggtgacc atgggtctgc tgggcttcga cggcggagcg
      721 gcgctgtcgc tcctcgacac cgccgtatgg ctgccaaccc ggcacgggga gtacgggctc
      781 gtcgagaccg ctcactccgc cgtcgccgac atggtcacag cacggctcgc ggttcgcccc
      841 gtaagcgcgc ccaccagcgg ctagccggcc atgccgaccc agcgcccccg gcaggcggtg
      901 atcctcgccg gcggtcgagg cacgcgtatg cgccccctca ccgacgaccg cccgaagccg
      961 atgatcgagt tcagcggccg gccattcatg gagtacatgg tcgagctcct ggcggagcag
     1021 ggcttcgagc gcgtgctcat gctcctcggg taccttccgg acgtcatccg cgaccatttc
     1081 ggggacggga gtcggttcgg tatccgaatc gagtattcgg tcaccgaccc ggatgacctc
     1141 accggtcgcc ggctccaggt cgccgatgac ctgctcgacc cgctcttcat gctgatctac
     1201 tgcgacaact attggccgct gaacatggaa cggatgtggg accactacca gcgggtcggc
     1261 gcccccgcca tggtcacgat ctatgccaac caggatggct attcgaaggg caatacgctg
     1321 gttggggccg atgggctcgt gcaggcgtac gatcgcagcc ggaccagcga gaatctgacg
     1381 ggagtcgaga tcagctacgc catctttccg aagcgattcc tggccctgct ccccgcggac
     1441 ggcgccgaac tcgtcgaaga ggccgtgtac ccgcgcctga ccgcggaccg actgcttggg
     1501 gcccatgtct cggagcaccg ctactacagc gtcggctcga tgcaccggat gccgctgacg
     1561 gaggcgttct tcgcgcgcag gccgacggtc atcctggacc gggacggcgt cctgaacgag
     1621 cggccgccgc gcgccgagta tgtccggacg ccggccgact tccagtggct cgacggcagt
     1681 ctcgaggccc tgcgcctttt caccgacgcc ggttatcagg tcgtggtcat ctccaaccag
     1741 gccggcatcg gccgcggcgt gatgaccgag tccgacctca ccgcggtcca cgatcgaatg
     1801 gtccgccagg ccgccgacgc gggcgggcgg atcgacgcca tctaccattg cccgcacgac
     1861 tgggatgccg ggtgcacgtg ccgtaagccc agtcccggga tgctgttcga ggcgcagcac
     1921 gacctgcagc ttgacctcag ccggacgccg tttatcggcg acgacgaacg agacggccag
     1981 gccgcatacg ccgctggagc gccgtacctc gccgttgacg acagcacgag cctgctggcc
     2041 cacgcccgtc ggctgacggc gtcagcgacc gcggcagtcc acgcatgagc gcccagcggc
     2101 gtgtgctcgt caccgggcac cgaggctacg tgggcgcggt catggcgccg gccttccgcg
     2161 aggcaggctt cgacgtggtg ggcatggatg tggatttctt ccgcgactgc gacctgttcc
     2221 cgctggcgga catcccgtcg atcgagaagg acatccgcga cgtcgaggcg tcagacctcg
     2281 aaggtttcga tgcggtcgtc catctggcgg ccctcagcaa cgacccgatc ggaaacctga
     2341 acgaagcgtg gacggcagac atcaacgtcg atgggacggt ccggctggcg gaggcggccc
     2401 gcgaggcggg agtcgggcgg ttcctgttct cctcctcctg catcatgtac gggatggccg
     2461 aggcgggcgc ggtcgacgag gactcgccgc tcaacccgca gacggagtac gcccggtcga
     2521 aggtcgtggc cgagcgggcg ctctcggccc gcgcaacccc ctcgttttcg ccggtctacc
     2581 tccgcaatgg cacgatcttc ggggtgtctc ctcggatgcg gttcgacacg gtcctcaaca
     2641 acctgaccgg catggcggtg accaccggcg cgatcacggt catgagcgat ggcaccccgt
     2701 ggcgcccact cgtccacgtg cgagacgtgg cccgggcatt catcgccgtg gtcgacgcac
     2761 cggtcgaagc ggtccatgac caggccttca acctcggggc cgacgcgctc aaccaccagg
     2821 tccgcgatct cgctcacgcc gtccagcgcg ccgtgcccga ggcctccctc gagatccgtg
     2881 ccgtcccgga cgccgaccag cgcacgtacc gaacgagctt cgcgaagttc gcccgaacgt
     2941 ttccgacctt ccgtttcgag cggaacgcgg acgatggcgc ggtcgagctg accgcggcat
     3001 tccgcgacct ggggctgacc tacgaagcgt tcaccagtga ccgcttcact cgtctcaagc
     3061 gcctgggccg gctcatcgag gacggctcgc tcgatgacgc gctccggtgg cgcatcgacg
     3121 aggggatggt cgcctgatga gcgggcgggg gatcgttctg acccggacgc cactccgcgt
     3181 cagctttgcc ggtgggggaa ccgacctggc cacccactac gaggccgacg atggggccgt
     3241 cttcagctcg accatcgaca agtacgtcta cgtgaccgtg aagcgccacg gggcggtctt
     3301 tgacgagaat gtccggctca actattcggt cagtgaatcg gtcagctcga tcgatgcgat
     3361 ccgaaacgac gtcgcgcgcg agtgccttcg cctgctcgag atcgaaccac cgatttacgt
     3421 gagcgtcgtc agcgacctcc ccgactcgtc gggcctgggc ggatcgagcg cgttcgcggt
     3481 tggcctgctg aacgcgctgc acatgtttcg cggtgagcag gtcacggcat cgcagctggc
     3541 tgatgaggca tcgaccgtcg agatcgagat gctcgcccag ccgatcggca agcaggacca
     3601 gtatgcggcg gcattcggcg ggctcaacct gttcgcgttc cggccgggcg gggacgtgac
     3661 cgtgcagccg catcgtggcg tcaatggatc ggtcggggag ctcttcgacc atttgctcat
     3721 gatctggagt gggcaccagc gtcgcgcgag ctcggtgctg ctcgaacagc aggaacgcac
     3781 ggctgagcat cgtggcgtgc ttgggcgcat gcgcgagcag gcatacgagc ttcaggccct
     3841 gatgtcgtca ccgacgccgg acttccgccg ctttggagcc gtgctggatg ccggctggaa
     3901 catgaagcgc ggattggcga gcactgtgtc gaatcccgag atcgacgggt ggtatgcggc
     3961 cggactcgag gctggggccc ttggcggcaa gctgtgcggc gcaggcggcg gcgggtttct
     4021 cgtcttcctg gcggagccag gggcccgtga tcaggttcgt gccgcgctgc cgggtctcca
     4081 gagcgtcccc gttcgcttcg aggcgcacgg ctcacgcgtc ctgttcgcgg aggttgactg
     4141 atggcgccaa tcgaacctcg accagacttc ttcatcgtcg gcgcgccgcg gtgcgggacg
     4201 accgccatgt acgagtacct ccgccggcat ccggaaatct tcatgcccga gcacaaggag
     4261 ccgatgtact tcggtgcgga ccttgcctcc atccatggcc ggctgaccga ggcggactac
     4321 ctgcgcctgt ttcgtccggc gacgaccggc cagcggatcg gcgaagcaag cacctggtac
     4381 ctgtactcca cggacgcgcc tcgagagatc agcgcgttct cgccggaggc gcagatcatc
     4441 atcatgctcc gcaatccggt ggacgtgatg tactcgctcc atggcgaagt ggtcttctac
     4501 ggtggcgagg agatcgagga cttcgaggag gcgttggccg cagagcctga tcgtcgcgag
     4561 ggtcgccgcc tcggaccgac ccgccgtccc gaggccctgt tctaccgtga caccgtgcgg
     4621 ttcgcggacc aggttgagcg ctacctcgat gagttcggct cggagcgggt ccagatcctg
     4681 ctgttcgacg acttcgttcg cgatccggcg gccgcgtacc tcgaggtgct caggttcctc
     4741 ggcgtggatg agacattccg tccctcgttc gagcgtgtga acgagtcgaa acgcccggtg
     4801 aactcgacct tccagtcgtt cgtcgttcgg cctcccggac cggtcgcccg gctgatccca
     4861 ttgctccgtc gtgccccctt ggcccaccag ctccgtgcgg cggttctgtc cgcgaacagc
     4921 cgctcgtacg agcggccgcc cctcgccgag ccactgcgtc agaggctcac ggccgagctc
     4981 gccccggagg tcgagcgcct cggagagctg atcggtcggg acctgtcccg ctggatgccg
     5041 gttgcgacgc ccggcccaaa agaggcccgt tccgtcgcat gagccggcca caggtggcca
     5101 gtcggcggat gaccctgtcc ctgccagccg cgctggcgcc cttccgctgg acgatcgggg
     5161 caacctcggg cctgtttgct gccaccctcc tgaccagcgc aaccggtttc gtcttctggt
     5221 ggatcgccgc ccagggctac tccgagtcgg cgatcggtct ggcaggagct gcggtgtccg
     5281 cgatgctgtt cctttcgcaa gtggccatgc tcggctatgg caccgccctg gccggcatcc
     5341 tccaccgaga ggagcggcca gcttcactcg ccgtcacggc caccctcgca gccggcatcg
     5401 cagggctgat cctgggtgtc ggatttgccg tcctggcacc gctcctctcg agcgagctgc
     5461 agccgctcgc cgcaccactc gcgatcgtga tcttcgccct cggcgtgtgc gtcgcggccc
     5521 tcacctcggt cctcgaccag gtcctggtgg ccgtgaacca gcggctcatg cagctcatcc
     5581 gcaacgtgat cttcggcgtc ggccgccttc cggtcctgct cgcgatcgcg gccgtggtgc
     5641 tgtggcccga tgggatgggg atctacgttg cgtgggtcgt cggactgatg ctgtcgctgg
     5701 ccgtcatcgt ggtcctcgtc cataacgccc agctgaccgg agatatccgg ccgctgatgt
     5761 gggggcgcct gcgggccatg gccccggacg cgctcgccca ccacgtggtc aatctgtcgc
     5821 gatcgggatc ggtctggctc ctgccgctgt tggtcacgat cgtcctctcc agcgaggaga
     5881 acgcggcgtt ctacatcgcg ttcctggtct cgaacctcat cctcctcctc ggcaaggagg
     5941 cgacgtttac cctctacatc gtgggcgcgc gagcgccgga aacgctgtgg cggcagattc
     6001 gcttcaccct cggcttctgc gccctcgcca gcgtcctggg aaccatcgtt ctgtctgtgc
     6061 tcggtccacg ggtgctcggg gtcttcggca gctcatatga aactgcgtac ccggtcctcc
     6121 tgatcctgtc gatcagcgcg ctgccggtgc tggtcaagga tcactggatc gccgtccatc
     6181 gcgttcgggg gtcggtttcc aaggcggccg tggtcggagt catcaccctt gcgatggagc
     6241 tagccggcgc ggtcgtggga gccctctggg gcgagcagct gggctttgcg ctgctgggct
     6301 ttgcgatcgg gcgactggtg gccctgctcg tccaggccgc attcatgctg ccgatgctga
     6361 tctcggtcat gcggccgccc gcgggcggca cgccggtaac gccctcggcg accgacgccg
     6421 atcccaccga agccgtattc gcatgagttc ttcgcccatc gccatctcgg tcatcgtctg
     6481 cgcgtttgcc gacgatcggt gggatgacct cgtgggggct ctgaactcga tccagcgtca
     6541 ggggctgaat gcggccgaaa cgatcgtcgt caccgaccac aatgacgggc tcggtgctcg
     6601 ggtggcggcg gagttcccgt gggcacgggt cgttccgaac agcggacagc agggtctctc
     6661 ggatgcgcgg aacacgggag tggccgcctc gagcgctccc attgtcgcgt tcctcgacga
     6721 tgacgcggtc gccagccccg gctggctgga ggcgcttgcc gacccgtatc gtgaccagcg
     6781 cgtgatcggg gtcgggggtg cggccatccc ggcctgggat gccaggcgac cggcctggtt
     6841 cccggatgag ttcgactggg tggtggggtg cagctatcgg ggtctgccaa cgcacctcgc
     6901 accgattcgg aacccgctcg gctgcaacat gtcgttccgc cgagaggcgt ttgccgcgac
     6961 cggtggcttc gatacgagtg tcggccgagt cggccgcgtt ccggtcggag gcgaggagac
     7021 ggagttctgc atccggctgc ggcagcagat gccgaatcac gtcctgctct accagccgac
     7081 cgccaccgtc cggcaccggg ttcgagccga acgagcgacg tggcgatact tcaaggcgcg
     7141 ctgcgtcgcc gagggccgtt ccaaggcgcg gatcacgaca ctcgttggcg ccggtgacgg
     7201 cctggcgtcc gaacggcggc acgcggccgt cacgctgcca cgcggggtgg cgcgccatct
     7261 gtccgtggcg ctccgggaac tgcagcctgc cggcgttgtg cgagccgggg cgattgtggc
     7321 cggcctcggg tgcacggtcg cggggtatgc cgccggtcgg atgggggccc gtcagtcccg
     7381 cacgccgccg aagccggagc ccacggtcgg cccagcggga tgagcccctc gcccggtagc
     7441 gcggccgggc ggagtcgccg gccgacccgg gtgctcatgg tcgcgcatcg attcccgccg
     7501 tacaccggcg gggtcgagac gcataccctc gaggtcgctc gtcgcgtcgc ggccgattcc
     7561 cggttcgagg tccacgtcct gaccacggat ctcgatcgca agctaccccc gcgggaggtc
     7621 gtcgacggag tccaggtcac ccgcgtgcgt gtcggcccgc cgggtaccga cctgtacatc
     7681 ggcccggaca tcttccggcg cgtccgatcc acgagcgccg acctcgtcca ttgccagggc
     7741 taccacacct tcgtaccgcc gctcgccatg gcggcggcgc aaagggcgcg gatcccatac
     7801 gtggtcaccc tccattccgg cggacactcg tcccgcgtgc gacgcgccat ccgcccggtg
     7861 cagctccgcg ccctccgcgg tcagctggcc ggagcggagc gcctgatcgc ggtatcgcgg
     7921 ttcgaggctc acgagttcgc gcgacggttg aacgtgcccg aggatcgttt tctcgtgatc
     7981 ccgaatggct ccgacatgcc agcggtggcg aacgccaagc tcggacctcg cgacgcgggc
     8041 ctgatcctgt cggtcggccg tcttgagcgg tacaagggtc atcatcggct gatcgatgcc
     8101 ctcccccggg tgcgtcggga gcgtcctgat gctcgtttgg tgatcctggg cagtggcccg
     8161 tacctctcgg agctccggca ccgggccgtg gaacggggcg tcggggacgc ggtcgagatc
     8221 cgctcggcat cgcggcccga ggtcgcgggc ctgatggcgc aggcccaggt cgtggcgctg
     8281 ctcagcgact acgaatccca ggggcttgcc gttcacgagg ccctcgctct cggctgccgg
     8341 atcatggtca gccgcagctc ggcgctggcg gaggtggcgg acctgcccca ggcacgcggt
     8401 gtcgcgccgt cagcgggctc ggacgagatc gctgatgggt tgctggctca gctggccgcg
     8461 ccgagcagca tcgccgaccc acccatcctg cccagctggg acgagtgcgc ggcccgggtg
     8521 atggacgtct accgggcggt gctctccggc gaggactcgg gtagcgggcc aatcgggtcg
     8581 acgacttcct gagcaggcga aagccggcgc tttgccgtct cgagcagccg gcggggggcg
     8641 acgagggtgg cgagcgcgta catcgcgtga taggggctca gcacggcccg cgggctccag
     8701 cgcagggcgg cggcaatgtc ccgagccgag ccaggtcggc cgcggccgcc ttcgagaacc
     8761 cgatgctggg cggcccgctg gaggtgcgat cgggcgatga tcgctcggag acggctcgct
     8821 tcggcgtttg agacggcgcg ccgatgctga tgggtccgaa ggccggccag cagggccacc
     8881 tcgatcggcg tccgctggtc gttctctgag cggttgaccc acagtcggaa cgagttgtcc
     8941 gcgtccggac ggaccatgaa gtcgagcagt ggctcgtcga tgtaggcaac tgatccgtag
     9001 gccgccgccc gaagcgacag ctcgacgtcc gctgagagtc ccgtctcggg acggaagaag
     9061 ccgccgcttt cgtcgagcac atcgcgcgcg acagcgaccg agcccacgtt ccacggtggg
     9121 ttgccatcct ggaggtggat gtcgaaccat ccggcggcgt cgtatacgcc ggctgcaacc
     9181 tgccaccggg ggcgggttcc gtaccagatg tggtctgcgg atcgaccctg ttcgtccacc
     9241 cgtcggcatg cggtcacgac catggccagt cggtcgccgt cgggcgccca gccgtcgatc
     9301 acggtcgcca tccgggccag gcagccgggg tgaagccgat cgtccgctcc gaggaactgg
     9361 acccacggct cgcgggtcag gagcgcggtc cggttgaagt tctcgtcgat ccgggcatgg
     9421 gtctgccaac ggtggtatcg gatccgcgga tccccgaacc gacggacgat cggctcgatg
     9481 ggctcggtcg aggcgttgtc cccgaccacc agctcccact ccgcatgcga ctgggcgagc
     9541 acgctctcga tcgctccggc aagccaccgt ccgtcgttgt agatcgggat gaagatcgag
     9601 aacctggcca tcgtcagccc gcggccgcga accgctcgcc ctgctcggtg cggagcttgt
     9661 ccacgcgccg gcccgtaggc agctcgacgt cggcgtagcc gatcacggcg tcgcgcggga
     9721 tcgcgcgtgt caggcggcac ccctccgcga tgcccatagg cagcagtccc tcgcccgcga
     9781 cgacatcggc gttgtcacat tggccatacg cggtgtaccc gccgattcca tcgagaacct
     9841 cgccggcctc gaggtcccgc ttggcggtgg ccaccacctc caccaccgga cccccttgtg
     9901 gggcgaggac gggatcgttc agcagaaccg cccgcgcaac cgacgtcggg acctcgaaat
     9961 ggcacaggtg ctgcggcgtg tagaacgagt acagggggcc gtccccgcgc ttgtacagct
    10021 tcatgtagtg ctgttcgagc ggatcgtcat gcgtcgccat cacgtacaca ccgggcgcag
    10081 gctgggcgcc gacgacgtag tccacgatcc cgccgaggcg cgtcagctcc tccacgtcat
    10141 atcgggtcgt caactcgtcc acgtgaccgg tgtggtccca cgcgtacaaa ccgcggcgag
    10201 gcgctcggaa accggtgccg ttggccacga tggcctgctc gaacgagacc ttcgtgccat
    10261 ccgcgaagct cgtcaccatc cggacgccga ggccccacgt ctgggcccag ccctcctgtg
    10321 tggtcggcgt cctgtacggg tcttgcagcc ccttgatgtt cccgcacacc aatggacgga
    10381 gaccgattcc ctgcacaaac cgataaaggt tggcctcgac tcccggctgg tcgccgtcgc
    10441 aggcagtcag gatgactccc gcctcgtcgg catgcacctt caggatcgga ccgaccgtcc
    10501 cgtccagctc ggcgttcatg aggatgacgt gcttcccatt ggcgatggcc gactcgacaa
    10561 cccgagcggc atgctccacg gtgccggtca cctcgaccac cgcttcgatg gagggggcct
    10621 ccgtcaccag gaacgggtca tcggtcacgg cgaagcgatt cgcccggatt gcggcctcga
    10681 catcctcgac cgtggccgca tggtcgacct cggtctgtcc ggcctcgtgg taggcgcgga
    10741 cggcggaatc gacagtgcgg ttcgcgatgg ctgacagtcg cagaccggga aaggcccgga
    10801 acatctggcg cgcgatgcct cgacccattg cccccgcccc gaccatcccg acctggaccg
    10861 gcctgccgct cgccgcgcgc tcggccagcg ctcggtcgac gatgatcatg gccgggccgg
    10921 cgtcagccca aggtgcggcc aggcggcatc cttctcggag atggtggtca tgggcagagg
    10981 ccattcgatg ccgaaggccg ggtcgtcgta tcggagcccg ccttcggctt cgggggtgta
    11041 gaactcgctc acgttgtaga gcagctcggt gacgtcctcc agcgcctgaa ggccgacggc
    11101 gcacccctcc gccacgtaga ggctgcgctg atctccctca tggagctcga cggtcgtgtg
    11161 ccgcaggtgg gtcgcgctct ccggccgaag gtcgacgacc gccatgaagg ccccaccccg
    11221 gatgcagcgg atgtgctttg cctcggcgaa cggcggccgc tgatagtgga ggccgcggac
    11281 ggtgccccga cgcaggttgt gggagaggtt tgcctggacg atgcgcgggt tcagcccgtg
    11341 ctccttgaac tcgttcacgt cgaacgcacg ggcgaagaac ccgcgttcat cttcacgcgg
    11401 ctcgagctcg agcaccatca cgccgctcag cggcgtcgtc gtgaatcgca tctcaccccc
    11461 tccgaggacg cttgggcaac gccccttctc cccgctggga gcctcccgcc gtcagcgagt
    11521 ccgacgcgct cggaccgcct ggacgttcag gactccgatg gtgcccaggg acgccatcag
    11581 caggaaggcg aacaggaggc cgaggtcgct ggacgaggct ggggccatgg cggtgtccgg
    11641 cagaccggtg ccgccttccg ttccgctttc cggcggcgtc tggggctcaa caggatcctg
    11701 cgggcatgtc tccgatcctt cgatccgtga tcggatctgt cggtcgccgt agcgatcctc
    11761 gaagaccagc gtctcgatgg caggcccgaa cacgaagtcg tactgcgcga aacacccggc
    11821 cgcggcgcca tcgcgtggca ggtccacggt gagggtgtgt gttcccgcgc cgaaccaccc
    11881 ggtgtccgag tcgaagagcg tctgggggaa cgagaaatcg gggccaggca gctcgtagct
    11941 tgcgagcgtg atctcgcagc tcgctggagt ctcgacttcg tccgcgatcg tgaattcgac
    12001 gctcacccac catgccttgg agtcggccgg ggtgttgacc gtcgtgacgt actgggtcac
    12061 caggccggac ccgaaggcat cggggcactc gaggtcggga tcggggtccg tgggtacgga
    12121 tgcgccgagg gcggccgaga caacgagcag ctggatggtc agcgccaggc caccggcgag
    12181 cgcccacctg tggcgccggg gctgatttga gagagggcgg ctcaacatgg acatttccct
    12241 tctggctggc gcgggacggc gctgccctgg tcgatggata aggacggcgg tagggtagcc
    12301 gaggcacagt acttttggga aggtccgcgg cggtcacgtg tgcggtgcgg aagggtctgg
    12361 ggcggtccgc aaatcaattg gtggtgcgcc gacgcaccgg agtaccagga cccgggacga
    12421 cgtgtgtcaa acgacggggc cccggctggt cgccggggcc ccgttatcgc tgagggttgt
    12481 ccgggctggc ccggccgaga gactacgggc gtggcacctc gatcgtcacc cagtcgacca
    12541 ggacgtgacc ggaagccggc ggcgggagta cctggcccgc catgtaggtc tcgacctgca
    12601 tgatgtagtg catcgggcca cgaggaacgt actgcgtgga tcggccaaag agccgaccgt
    12661 ctacgaagaa ttcggtggtg cctgactgcg gccgccaggc gacggtgtac gtgtgccaac
    12721 cctggagggc gacgcccgtg tcatacgcgt cctgctcccg cccactggac ggcctggggt
    12781 cgtagtgcat gaacgcccgc gcggtcgcgg acccgccgtt cagcttgcct tccgggaagt
    12841 cgatctcgcc acgctcgttg cccgagccct ggctcggcca cagcaagaat gccagcttgt
    12901 agccggggat cacgtccgcg cgcatgcaga tcgagatccg ctggccgagg gtgtcgccaa
    12961 cgagggagat cggagccgcc acgtagcggg tgacgccctc ggaatggacc cagatgtcca
    13021 tcaggctgtc gtgctggctg gtggtcttgg gcgagctgta cgtgccgcgc cgggacgagt
    13081 ccttccaccc atcgggccgg gctcgccagt ggccgcctgg ctgttcccac gcgccaccgt
    13141 cgcgacccca ctggcccaac gggatgttct cggagaaggt gtcggaaacg actcgctgcc
    13201 agcctgcggg tgccgtggcc ggcatcggac cacaggtgct gggtgatgga tcgtcggtgt
    13261 caggcggtgg gggcacggtg gtcggaacgg acccggtgtt cacgaccctg aaggtcaacg
    13321 agaccggcgg attggccggt ccctgcgccc ggcgctgggc gaaggcggtt gctgtgacgg
    13381 tgtagctacc gacggcgggc gtccaagcgc ggtagtcgcc ggcttcgtcg ccagtcatcg
    13441 aatatggctg gccgttctcg acccgggcgc cgccgccatt gacggtcacc cggacgctgc
    13501 cgacggccgc ggcggtgttc acccggacgt tcagtcgtcg ggtcggaagc gacgcgagat
    13561 ccagggtcgc gcccggctga atggggttga acccggcaat cggctgatcg gtgtcggcgt
    13621 tgatcaacgt gaagccggtg acgccaggaa cggcggcgac cgccgtgcct ggggaggcgg
    13681 tgaacatgag tgcgagggcg aggcccacaa ggggcaggcg gaagcggacg aggtgcaggg
    13741 ccatgacgtt ccttccggtg acggttgccc ccaagggttg cggcacaggc ggccggccac
    13801 caccgggtcg atggtcccct gtcgcgttgc gctcatcggg cccccgtacc aaggggccaa
    13861 caggcgccgc cgtcggtcct cgaccgtttg gcccccggcg cgaaaggaag gcctcggggg
    13921 ccgaggatgc gctcgtcgac gaaagccgcg agaatccgga ctctgttctg gcgccagccc
    13981 acgccggtcg ggccttacac cctatgcagg agaagcaccc gtgggtcgat tccatcgctc
    14041 cgtgcccatc gcgacggcgt ttctgatcct gttcgcgggc gtcctcctgg tgggggacaa
    14101 aggtcccatc ctgaccggcg gcggaccaag ccactcgccc gcgccatcag tcctatcgcc
    14161 gtccgtcagt ccggccgcgg gctcgaacca gccgtcgcca tcgggcgatc cgtcgttgcc
    14221 attcgggccc ggcgacccct ctctggacgg ctcggtctgg tatccgccgg ccatcccggt
    14281 cggcgcgtac gccaccgtcg cggcggtcaa ctcgtcggcc cagaatcgcg accgcgccga
    14341 cttcgtcgtc cagggaacgg ctgacgacga gatcaacgcc gcgctggccg atctcgcgcg
    14401 catcggcggc ggacaggtcc agcttctcga gggtctctac gagctcgcgg cgccgattac
    14461 gatctcgggt gacggcctgg ccctcgtcgg cgtgaacgtt ggcaatgcgg ccggctattc
    14521 cgaggcggcc ctcggctcgc agatcgtgcc agcagccgga tttccggacg acgcattcct
    14581 ggtcaacgcg acggcagagg catacggccc actcatcagc ctgatccacg tcgatgggct
    14641 gacccgcgcg cagggaatga acatcgaggg gtcccgcccg accgtgtcac tcaacgcggt
    14701 gtcccagtcg agcggggtcg ggatccgatt tgccggcgag agcaccgggc agcgtcccta
    14761 cgacggattt gtcctattca atcgggtgtt cgaggggctg ggcatcggga tcctccatga
    14821 ccagcgttcg ggggacatgc tgatcgaggg aaatgtcgtc ttccggaacg gcggcgacgg
    14881 cttcctcagc cgtggagcct cacagatgta ctcgctcaac cacgcataca acaatgccgg
    14941 cgtcgggatt cggctggtcc cgggctgtgt gcgaacacgt ctgaccagca acaagtggga
    15001 gggcaacgac ctgggtggcc tcagcatcga gggcgggagc ggattcacgg tcatcggcga
    15061 caccttcgcc gacaacgaca gccccagcgg gacgagcgcc gcgcatatcg agctcggcgt
    15121 ccgaggccca accgagacga tcggcgtcat gctgtccaac ctggccttcg gcaagggcga
    15181 cgatgggaat ccgtatctca tccacttcgg ctcgctggcc cgcgatgtcc acgtggg
//
LOCUS       DASYGU010000235         5187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1322829, whole genome shotgun sequence.
ACCESSION   DASYGU010000235 DASYGU010000000
VERSION     DASYGU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322829"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..890
                     /locus_tag="VGO32_06655"
     CDS             153..890
                     /locus_tag="VGO32_06655"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV7200465.1"
                     /translation="MPFNPTAAADGLEAAIRAVGSPGRAESEKKYLKSDLTFLGATVW
                     QTRDTVKAYLRETTLDHASLEALLTTLWSAPIFERKMAAVMLLDLNPGLLDAADLPLL
                     ERLLRESLTWALVDALAGDIVGRVRLADPSVEPTLDRWATDPDFWIRRSSLLAELKAL
                     KKGADMDPFLRRADLMLDEKEFFIRKAIGWVLREVGKKRPDEVIAWLAPRTARASGVT
                     MREAIRYLPAADGARLMAAYKEHRPAA"
     gene            1038..2888
                     /locus_tag="VGO32_06660"
     CDS             1038..2888
                     /locus_tag="VGO32_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7200466.1"
                     /translation="MPSPRQPSAGPVRSRRPALLALVFGIFLVLIGLTATALVVVTAM
                     HLSSATLNAVVSRDRSLVELFVNGNVTAADIDQNGPSRASAEALEAKLGTLTAEDEIL
                     RIDLRGLDGAILASSDPELRGTNPGASTALQSAIAGEPSISLLDAVAATDAGDTVLDT
                     SQVVQEYLPLLSDEGEPLAVVAVWRDAAPLLSGVDAARRDIVIVTLAAAILLGVVLLG
                     VFWAAQKRILRQQADLVEAERRDPLTNLLNHGAVVTLMAEAVETARAKGRRFGLALVD
                     IDNFRLFNDTHGHDAADQVLLRVADWLQREDGEDKAIARYGPDEFLLVLPGADRVDME
                     AAVNRIRTGLSGVSVQFGESEQLPVTVSAGICLYPDHADSVTELLSAVTVAVGEAKAS
                     GGDAVCVAQLGDERQKAVSGSFDVLQGLVIAVDSKDRYTKRHSEDVARYAVFLAGQLG
                     LDQDLRRTIHLAGLLHDVGKIGIPDHLLRKPSKLTAEEFDVFKQHVALGDAIVRDIPN
                     VDLVRAGIRHHHERWDGNGYLEGLEAGEIPIIARVLAVADAFSAMTTTRPYRKALSVE
                     EALKRLGDAAGRQLEEDLVVAFIKGMETAPDAPIPAERPSGIWRPTEWVA"
     gene            2891..3865
                     /locus_tag="VGO32_06665"
     CDS             2891..3865
                     /locus_tag="VGO32_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200467.1"
                     /translation="MRRVWVRPVSVVATLLLGILALAAPVSAVVGWTLVGGPLIATMF
                     TSTTYTFTATNLTYGSGIGCVEIQLPDQFVIESLGDPVPSDSGHDWSSGLYGGTNWVL
                     AHATGGGGRLDILESVTFTITATATSAGDWFWNTHAHRRQDCTGPDIEAGIWPMVVNP
                     IAPPSAPPTPVPTAVPTVVPTPIPTLVPTPPPLPIGTPQPTATPAASSSGTPRPTPTG
                     SPRPTPEVIAGAPAAPPGGSGTPVPLGRMAPLAESDTSAIGLGTEVFALLEGPLVWFV
                     PSAVMGGPGLLILLFVALQAGGALAWIPAVKRMSGEPLPVQRRRRPGS"
     gene            complement(3898..>5187)
                     /locus_tag="VGO32_06670"
     CDS             complement(3898..>5187)
                     /locus_tag="VGO32_06670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV7200468.1"
                     /translation="PVETAVAAAPSEPPSAAPAPVAEDEPALAAVAAAPAAAPTAPEP
                     EPAPAPAVAASSDAGDAGMRMTPAVRRLLREHNLEVTQIIGTGAGGRVTRDDVLAYVA
                     ARDGAPAGESAAPAPPPEPAPAPVAVAEAPAPAAPPRAPVTPAQAPTPVAATPAATPA
                     PTPTPAAPSATSAPAPAPAPPSAPAPEAIPAPTGGDVEWPMTQMRKGIAAKMTQVKQT
                     VPHAYTVVEVDMTNVVRWRDANNEAYKAREGAGISFVAVVIKAVTEALRAHPTLNSQF
                     AGDRIILKSRLNIGIAVAVDNGLVVPVIADADQLSISGVNRKVQDLAARARANRLKLD
                     EIQGGTFTVNNTGWFGSISSMPIINAPEVAILSMEAIVKRPKVIEVDGSDVIAVRHLM
                     NMTCSFDHRVLDGAQVGYFMADVRKRLEGWETDAPLS"
BASE COUNT          774 a         1808 c         1791 g          814 t
ORIGIN      
        1 gtatccggcc caccggggta catcacctcc gacggtgcgc ggtgtcccgc gccggccccg
       61 ccatccggat cgaccaggga cagctcgccc agggacgaga aatcatgcgg catcggggag
      121 agtctagcga aggccgcgac tagactcgga tcatgccctt caacccgacg gccgcggcgg
      181 acgggctcga ggccgcgatc cgtgccgtgg gctcgccggg ccgggccgaa tccgagaaga
      241 agtacctcaa gagcgacctc accttcctgg gcgccacggt ctggcagacc cgggacacgg
      301 tgaaggcgta cctcagggag acgaccctcg atcacgcctc gctggaggca ctgctcacga
      361 ccctttggtc cgcaccgatc ttcgagcgga agatggcggc ggtcatgctc ctggacctca
      421 atcccgggct cctggacgcg gccgacctgc cgctcctgga gcggctcctc cgagagtcct
      481 tgacctgggc cctggtcgac gccctggccg gcgacattgt cgggcgcgtc cgcctcgccg
      541 acccatcggt cgaaccaacc ctggaccgat gggccaccga ccccgatttc tggatccgac
      601 gctcgtcgct gctggccgag ctgaaggccc tcaagaaggg cgcggacatg gatccgttcc
      661 tccgtcgggc ggacctcatg ctcgacgaga aggagttctt catccgcaag gcaatcggct
      721 gggtcctgcg tgaagtgggg aagaagcggc cggacgaggt gatcgcgtgg ctggcgcccc
      781 ggacggcccg cgcgtcgggc gtgaccatgc gtgaggcgat ccgatacctg cccgcggctg
      841 atggcgcgcg gctgatggcc gcctataagg agcatcgccc cgccgcctga gaggcgcgac
      901 gggccgtttc gagcgccaaa ggtacgggcc tggtcccagg cccgtctggt gcctctggtc
      961 aacgcgaaca ggcccccgaa cacggggtga ccgattctcc tattcactcg ggtgtgactg
     1021 atgggtaggt tcggcccatg ccgtcaccgc gccagcccag cgccgggccg gtccgatctc
     1081 gtcggccggc cctcctcgcg ctcgtcttcg gcattttcct ggtgctcatc gggctcaccg
     1141 ccaccgcgtt ggtggtggtc accgccatgc acctgtccag cgcgaccctg aacgcggtgg
     1201 tgtcgcgcga ccggtcgctc gtggagctgt tcgtgaacgg caacgtcacg gcggccgaca
     1261 tcgaccagaa cggtccgagt cgagcttcgg ccgaagcgct ggaggcgaag ctcggaaccc
     1321 tgaccgcaga ggacgagatc ctgcggatcg atctgcgcgg actggacggg gcaatcctgg
     1381 ccagctcgga tcctgagctg cgcgggacga atccaggcgc ctctaccgcg ctccagagcg
     1441 caatcgcggg cgagccgtcc atctccctcc tggacgccgt ggcagcaacc gacgccggcg
     1501 acacggtgct tgatacgagc caggtggtcc aggaatacct cccgctcctg tctgacgaag
     1561 gggagcccct ggccgtggtg gccgtctggc gcgacgcagc accgctgctc tccggcgtgg
     1621 atgccgcccg tcgggacatc gtcatcgtca ccctggccgc cgccatcctg ctcggggtcg
     1681 tgctcctcgg cgtcttctgg gccgcccaga agcggatcct tcgtcagcag gccgacctgg
     1741 tcgaggccga acgccgcgat cccctcacca acctcctgaa tcacggcgcg gtggttacgc
     1801 tcatggccga ggcggtcgag actgcacggg ccaagggccg caggttcggc ctcgcgttgg
     1861 tggacatcga caacttccgg ctgttcaacg acacgcacgg ccatgatgcc gcggaccagg
     1921 tcctgctccg cgtggcggac tggctccagc gcgaggatgg cgaagacaag gccatcgcgc
     1981 gatacggacc ggacgagttc ctgctcgtcc tgcccggcgc cgatcgggtc gacatggagg
     2041 ccgcggtcaa tcggatccgg accggcctgt ccggcgtcag cgtgcagttc ggtgagtctg
     2101 agcagctgcc ggtcacggtc agcgcaggca tctgcctgta tcccgaccac gccgactcgg
     2161 tgaccgagct cctgtccgcg gtgacggtgg ccgttggtga ggcgaaggcc agcggcggcg
     2221 acgcggtgtg cgtggcgcag ctcggcgacg agcgccaaaa ggcggtctcg ggcagcttcg
     2281 acgtcctcca gggactggtc atcgcggtcg actccaagga ccgatacacc aagcgccatt
     2341 cagaagacgt ggcccgctat gcggtattcc ttgccggcca gctgggcctc gatcaggacc
     2401 tgcggcggac gattcacctc gcggggctcc tgcacgacgt gggcaagatc ggcatcccgg
     2461 accacctcct ccgcaagccg agcaagctca ccgccgagga atttgacgtc ttcaagcagc
     2521 acgtcgccct gggcgacgcg atcgtccgcg acatcccgaa cgtggacctc gtccgggccg
     2581 gcatccgcca ccaccacgaa cggtgggatg gcaatggcta ccttgagggc ctggaggctg
     2641 gggagatccc gatcatcgcc cgcgtgctgg ccgtggcgga cgccttctcg gcgatgacca
     2701 ccaccaggcc atatcgcaag gcactgtccg tcgaggaagc cctgaagcgg ttgggtgacg
     2761 cggccggccg gcagctcgag gaagacctgg tcgtcgcatt catcaagggc atggagaccg
     2821 cacccgacgc cccgattccc gccgagcggc cgtccggaat ctggcgcccg accgaatggg
     2881 tggcctgacc gtgcgacgcg tttgggtccg accggtctcg gtggtggcga ccctgctcct
     2941 cgggatcctc gctctcgccg ctccggtttc ggccgtcgtg ggctggaccc tggtcggcgg
     3001 cccgctgatc gccaccatgt tcacttcgac gacctatacg ttcactgcca cgaacctcac
     3061 ctatggcagc ggcatcggct gcgtggagat ccagctcccc gaccagttcg tgatcgagag
     3121 cctgggcgat cccgtgccgt ccgactccgg ccacgattgg agctccggcc tgtacggcgg
     3181 gacgaactgg gtcctggcgc atgccaccgg cggcggcggg cgcctcgaca tcctcgagtc
     3241 ggtgacgttc acgatcaccg ccacggccac ctcggccggt gactggttct ggaacaccca
     3301 cgcccaccgc cggcaggact gcaccggccc ggacatcgag gccggcatct ggccgatggt
     3361 cgtgaacccg atcgccccgc cgtcggcgcc accgactcca gtccccacag ccgtacccac
     3421 ggtggtcccg acaccgatcc ccacgctcgt tccgacaccg ccaccgctcc cgatcggcac
     3481 cccccagccg acggcgacgc cagccgcgag ctccagcggg accccgcgac cgacacccac
     3541 gggttcgcca cggccaaccc cggaagtcat cgcgggagcc ccagccgcgc cgcccggtgg
     3601 gtccggaacc cctgtcccgc tgggtcggat ggcgccgctt gccgagagcg atacgtcggc
     3661 aatcgggctc ggaaccgagg tgttcgcgct cctggaaggt ccgctcgtct ggttcgtgcc
     3721 cagcgcggtg atgggtgggc cgggcctcct gatcctgctg ttcgttgcgc tgcaggcggg
     3781 tggcgccctg gcctggatcc ccgccgtcaa gcggatgagc ggcgaaccgt tgcccgtcca
     3841 gcgccgccgc cgcccgggca gctgaggcgc cctcagccga agctcggctc caccgggtca
     3901 gctcagggga gcgtctgtct cccagccctc gaggcgtttg cgcacgtcgg ccatgaagta
     3961 gccaacctgc gccccgtcga ggacacggtg gtcgaacgag caggtcatgt tcatcaggtg
     4021 ccgcacggca atcacgtccg agccgtcaac ctcgatcacc ttgggcctct tgacgatggc
     4081 ctccatggac aggatggcca cctccggcgc attgatgatc ggcatcgatg agatcgatcc
     4141 gaaccacccg gtgttgttca cggtgaatgt cccgccctgg atctcgtcga gcttgagccg
     4201 attggcacgg gcccgggcgg ccaggtcctg gaccttgcga ttgaccccgc tgatcgacag
     4261 ctgatcggca tcggcgatga ccggcacgac caacccgttg tcgaccgcca cggcgattcc
     4321 gatgttcagc cggctcttca ggatgatgcg atccccggca aactggctgt tcagggtcgg
     4381 gtgcgcgcgg agggcttcgg tgacggcctt gatgacgacc gccacgaagc tgatgcccgc
     4441 accttcgcgc gccttgtagg cctcgttgtt cgcgtcacgc cagcgcacga cgttggtcat
     4501 gtccacctcg accacggtgt aggcgtgcgg gaccgtttgc ttgacctggg tcatcttggc
     4561 cgcgatgccc ttgcgcatct gggtcatggg ccactccacg tcgccgccgg tgggagcggg
     4621 gatggcctcc ggtgccgggg ctgacggggg tgccggggcc ggtgcggggg cgctggtcgc
     4681 ggatggcgcg gccggtgttg gcgttggagc cggagttgcc gccggagtcg ccgcaaccgg
     4741 ggtcggggct tgagctggcg tgaccggagc ccgaggaggc gcggcgggag cgggagcctc
     4801 agcaacagca acaggagcgg gagcgggttc agggggcgga gccggggcgg ccgattcgcc
     4861 agccggggcg ccatctcggg cggccacgta ggcgaggacg tcatcgcggg tgacgcgtcc
     4921 accggctccg gtgccgatga tctgggtcac ttcgaggttg tgctcgcgaa ggagacggcg
     4981 aacggcgggc gtcatccgca tccccgcgtc gcccgcgtcg ctgctggcgg ccacggcggg
     5041 agcaggagcg ggttcaggct cgggtgcagt tggcgccgct gcgggggccg cggccacggc
     5101 cgcgagggca ggctcgtctt cggcgaccgg tgcaggcgcg gccgacggcg gttcggacgg
     5161 cgccgcagcc accgcggttt cgaccgg
//
LOCUS       DASYGU010000236         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_293326, whole genome shotgun sequence.
ACCESSION   DASYGU010000236 DASYGU010000000
VERSION     DASYGU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293326"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGO32_06675"
     CDS             <1..592
                     /locus_tag="VGO32_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200469.1"
                     /translation="RRIEGTGQLGETASWTGSSAVVVDALTTVFTVHANDAEVVVDVY
                     GLGAPAEQGMEADLPETEQAAQEALVALRADLLDLETWIPDDEWADSEWQPYEPQAIR
                     LLIGSADDEEPDPDASGGAPVEWPGTIPPAELGQESPLQELRCGVVAGAEAGTWYEAL
                     REANQMTRFTHDGHLYRVVPRPLLPDEELTCGSLEG"
     gene            639..1541
                     /locus_tag="VGO32_06680"
     CDS             639..1541
                     /locus_tag="VGO32_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200470.1"
                     /translation="MRRPRHALLGVPLALALGACSASSPAPYSGPVPHPAGSELVVRI
                     DWTGGFVPYESLFTSLPQFTLLGDGRVFVEGPQIAIYPGPALPNVQVRQLTEEGIQSI
                     LSRIAETGLFTESSSWDAATQFIADANSTVFTVRADGREVVLDIYALGFLTDPVSDSL
                     DITAAERDAHERLQRLENDLLSLDTWIPADDWADDAWQGYIPDAMRLLVANIDDRPPN
                     PDDLGSEPMAWPSATPPDAFGTATDVHDVRCGVASGADAETWHAALGQANQLTPWSYD
                     GHLYSVTARPLLPDEPLDCGLPEA"
     gene            1696..2748
                     /gene="coxB"
                     /locus_tag="VGO32_06685"
     CDS             1696..2748
                     /gene="coxB"
                     /locus_tag="VGO32_06685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV7200471.1"
                     /translation="MRARAPHRWRTILTRLVAVSLPALLLAGCIFPPEPMTTEAEEVR
                     TLFLVIFALGALVFVGVEGFLLFAIFRYRRRDDRLPVQHHGNTKVEIVWTVIPSVIVL
                     ILFVTSMLTLGNISAKSDDPVTIEVEGFQWQWAFHYSTGYEVTGTPADPPVLVLPATE
                     PVRLVLRSDDVIHSFYVPAFLVKLDVIPFGQDQLPNALEFTVTEPGTYRGQCAEFCGD
                     LHADMTFTVDARSPADFATWLEEASSATPTPPEPSLPPDATIVELSADNIEYSTHDLE
                     VPAGEPFIIRFTNFEDVVHNVAVYDGQEALFNGAELTGPDATIDYLVPALAPGEYTFI
                     CDFHPIAEMTGTLTAH"
     gene            2767..>3345
                     /locus_tag="VGO32_06690"
     CDS             2767..>3345
                     /locus_tag="VGO32_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV7200472.1"
                     /translation="MATTTLEPGDKTYERSWVLDWLTTVDHKKIGVLYIVNAFVFFFF
                     AGLLALVIRSELAVPGLQFVDEGTFNQAFTMHGTLMIFLFIVPIFSGFGNYIVPLMLG
                     APDMAFPRINALSFWMLPLGGLLIISGYLVGGAAAAGWTGYNPLTQARFEPGDGTDLW
                     IMGLIVVGTASILGSVNFLTTIFKMRAPGMTLF"
BASE COUNT          521 a         1206 c         1073 g          545 t
ORIGIN      
        1 gcgccggatc gagggcaccg gccagcttgg cgagaccgcc tcgtggacgg gatccagcgc
       61 ggtggtcgtc gatgcgctga cgacggtgtt cacggtccac gccaacgatg ccgaggtggt
      121 ggtcgacgtg tacggcctcg gcgcaccagc cgagcagggg atggaggccg atctgccgga
      181 gaccgagcag gcggcgcagg aagccctggt cgcgctccgc gccgatctcc tggacctgga
      241 gacctggatt ccggatgacg aatgggccga ctccgagtgg cagccctacg agccccaggc
      301 catccggctc ctgatcggga gcgctgacga cgaggagccg gaccccgacg cctcgggagg
      361 cgccccggtg gagtggccgg gcaccatccc gcctgccgag ctcggccagg agagcccgct
      421 ccaggagctg cgctgtggcg tggtcgcggg cgccgaggcc ggcacctggt acgaggcgct
      481 ccgcgaggcg aaccagatga cccgctttac gcacgatggc cacctgtacc gggtggtccc
      541 gcgtccgctc ctgcccgacg aggagctgac ctgcgggagc ctggaaggct gatcgcgctc
      601 ggacggaacc acgtcgggcc gccgaacgtc aggaaggcat gagacgccct cgtcacgccc
      661 tgctcggagt tccgctggcc ctcgcgctcg gcgcctgcag cgccagcagc ccggcgccgt
      721 acagcggtcc cgtcccgcac ccggccggat ccgagctcgt ggtccgcatc gactggaccg
      781 gcggcttcgt tccgtacgag tccctgttca cgagccttcc ccagttcacc cttctcggtg
      841 acggccgcgt cttcgtcgag ggcccgcaga tcgccatcta ccccggcccg gcactcccga
      901 acgtccaggt tcgtcagctg accgaggagg gcatccagag catcctgtcc cggatcgcgg
      961 agacaggcct gttcaccgag agcagctcgt gggatgcggc gacccagttc atcgccgacg
     1021 caaactccac ggtgttcacc gtgcgtgccg acggccgcga ggtggtgctc gacatctacg
     1081 cgctcgggtt cctgaccgat ccggtcagtg attcgctcga catcaccgct gccgaacggg
     1141 acgcccacga gcggctccag cgcctcgaga acgacctgct cagcctggac acctggatcc
     1201 cggccgatga ctgggccgac gacgcatggc agggctatat cccggacgcc atgcggctgc
     1261 tggtcgccaa catcgacgat cggcccccga acccggacga cctgggcagc gagccaatgg
     1321 cctggccatc ggccaccccg cctgatgcgt tcggcacggc aaccgacgtg cacgacgtcc
     1381 gctgtggcgt ggcgagcggc gccgacgccg agacctggca tgccgccctg gggcaggcga
     1441 accagctcac cccgtggtca tacgacggcc acctgtactc ggtgaccgcc cggccgctcc
     1501 tgcccgacga gcccctcgac tgcgggctgc ccgaggcctg atccgcccgg cccacccgag
     1561 acgtcgccag gcgtctgagc cggcgggtac aatgccggcc gtgccgcggt cgaggacgat
     1621 cgaccggtag gcctgatcgg ggcgcgtcgg cgtggtccga accgccttcc acacggggag
     1681 cgactcgtcg gactgatgcg agcacgagca ccacaccggt ggcgaacgat cctgacccgt
     1741 ctggtggcgg tcagccttcc ggccctgctc ctcgccggct gcatcttccc gcccgagccg
     1801 atgacgaccg aggcggaaga ggtccgtacc ctgttcctgg tcatcttcgc cctcggcgcg
     1861 ctggtcttcg tcggggtcga ggggttcctc ctgttcgcca tcttccgcta ccggcgtcgc
     1921 gacgatcggc ttcccgtcca gcaccacggc aacacgaagg tcgagatcgt atggaccgtg
     1981 atcccgagcg tgatcgtgct gatcctgttc gtgaccagca tgctcaccct cggcaacatc
     2041 agcgccaaga gcgacgaccc ggtgaccatc gaggtcgagg gcttccaatg gcagtgggcc
     2101 ttccactact cgaccggcta cgaggtgacc gggacgccgg ctgatccacc cgtgctcgtc
     2161 ctgcccgcca ccgagccggt ccggctcgtc ctgcgcagtg acgacgtcat ccactcgttc
     2221 tacgtcccgg cgttcctggt gaagctcgat gtcatcccct tcggtcagga ccagttgccg
     2281 aatgcgctcg agttcaccgt gaccgagccc ggcacctatc gaggccaatg cgccgagttc
     2341 tgcggcgacc tccatgccga catgacgttc accgtcgatg cccgatcgcc ggccgacttc
     2401 gcgacatggc tcgaggaggc gtcgtccgcg acaccgacgc cgccggaacc ctccctcccg
     2461 cccgacgcca ccatcgtcga gctgtccgcg gacaacatcg agtacagcac ccacgacctc
     2521 gaggtgcccg ccggggagcc gttcatcatc cgcttcacga acttcgagga cgtcgtgcac
     2581 aacgtggcgg tctacgacgg gcaggaagcc ctgttcaatg gtgccgagct caccggcccg
     2641 gacgccacga ttgactacct cgtccccgcg ctcgcgccgg gtgagtacac cttcatctgc
     2701 gacttccatc cgattgccga gatgaccggt accctgaccg cgcactagga gaacgagcga
     2761 agaccgatgg cgaccacgac cctggagccc ggcgacaaga cctacgagcg atcgtgggtc
     2821 ctcgactggc tgaccacggt cgaccacaag aagatcggcg tcctgtacat cgtcaacgcg
     2881 ttcgtcttct tcttctttgc cgggctgctg gcgctggtca ttcgcagcga gctcgccgtc
     2941 ccgggtctcc agttcgtgga cgagggcacc ttcaaccagg cgttcacgat gcacgggacg
     3001 ttgatgatct tcctgttcat cgtgcccatc ttctcggggt tcgggaacta catcgttccg
     3061 ctcatgctcg gcgcgccgga catggccttc ccacggatca acgccctgtc cttctggatg
     3121 ctgccgctgg gcggcctgct gatcatctcg ggctacctgg tcggcggcgc ggcggcggcc
     3181 ggttggaccg gctacaaccc gctcacccag gcccgcttcg agccgggcga cggcacggac
     3241 ctgtggatca tgggcctgat cgtggtcggc accgcctcga tcctgggctc ggtgaacttc
     3301 ctgaccacga tcttcaagat gcgtgcgccg gggatgaccc tgttc
//
LOCUS       DASYGU010000237         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_293702, whole genome shotgun sequence.
ACCESSION   DASYGU010000237 DASYGU010000000
VERSION     DASYGU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293702"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VGO32_06695"
     CDS             <1..805
                     /locus_tag="VGO32_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEV7200473.1"
                     /translation="SDVGRLQGGQQVELTGTRSTKSGMLSLRVTARAVVLGAQAEPSP
                     ARRATGRIREADEATLVIVRGLVKDGPRRTTGGGMSFTVNDGSGPVRVFVAAGTGMTA
                     RHVPAGAWIELRAVVGQQTTGAQPSAGYRLWPRDRADATVIARPRVGGGGSGSTSPGT
                     TPHVIAPRPTVSPHPAVLLTRPRLSGSAALAIGSGSGPHAKPAGAPFAPSIPVPLAAG
                     LGGVAGLLTLAWRHGTLGRARLELEQRTGAIRAARGDGEAEDESYTSAP"
     gene            802..1605
                     /locus_tag="VGO32_06700"
     CDS             802..1605
                     /locus_tag="VGO32_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV7200474.1"
                     /translation="MSEPSAADAPRTGSRQYHIALERGEVAEYIVLVGDPGRVAKVAS
                     RFDRVDLERSNREITTTTGVFQGMPISAMSTGMGPDNVEILVSEVLEITDQPTFVRIG
                     SSGALQPSIGLGDLIISTGAVRLENTTDFYVHPGYPAIAHRDVVWALEAACQAAGYAH
                     HLGITATAPGFFAPQGRAMRTLPVRYPDLTEELRRQGVVNLEMESSALFVLAGLAGSR
                     AGTICAAYAQRTDGSFIEGADKEAAEARCIDAGLAGIHRLWGKDQGGGS"
     gene            1641..1784
                     /locus_tag="VGO32_06705"
     CDS             1641..1784
                     /locus_tag="VGO32_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEV7200475.1"
                     /translation="MATDGYCVKCKTKRTMKDDRKITMKNGKPATQGICPVCGTKMFK
                     IGG"
     gene            1859..>2172
                     /locus_tag="VGO32_06710"
     CDS             1859..>2172
                     /locus_tag="VGO32_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV7200476.1"
                     /translation="MLAEDRAPVLAIDLGGTLIRVAHLGPDLTVAHREVIPTMPTVGV
                     EGVLERMISTARSVLAAAEAAGLPAPAAVGVASPGPLDPQRGVVHQPPNLPGWDEVPL
                     AHR"
BASE COUNT          331 a          781 c          761 g          299 t
ORIGIN      
        1 gtcggacgtc ggccggcttc agggtggaca gcaggtcgag ctgaccggca cccgatcgac
       61 gaagtcgggg atgctgagcc tgcgagtcac ggcacgggcc gtcgtgctgg gcgcgcaggc
      121 cgagccatcg cccgcccgtc gagccacggg acggatccga gaggccgatg aggccacgct
      181 cgtcatcgtc cgcgggctgg tgaaggacgg accacgacgg accaccggcg gcgggatgtc
      241 gttcaccgtg aacgacggga gcggcccggt ccgtgtcttc gtggccgccg gcaccggcat
      301 gacggcgcgc cacgtgcccg ccggcgcgtg gatcgagctc cgagccgtag ttggccagca
      361 gacgaccggc gcgcagccga gcgcgggata ccggctgtgg ccgcgggatc gggccgacgc
      421 cacggtcatc gcccgccccc gggtcggcgg cggcggatcc ggctcgacct ctcccggcac
      481 cacgccgcac gtgatcgcgc ctcggccgac ggtttcgcct cacccagccg tgctcctgac
      541 ccgaccgcgc cttagcggga gcgcagccct ggccatcgga tcggggagcg gcccgcacgc
      601 gaaaccggcc ggcgcgccgt tcgccccgtc catcccggtc ccgctcgccg cgggactcgg
      661 tggcgtggct ggactcctca ccctcgcgtg gcggcacggc acgttgggcc gggccaggct
      721 cgagctcgag cagcggacgg gcgccatccg ggcagcgcga ggcgacggtg aggcagagga
      781 cgagtcctat acttccgccc cgtgagcgag ccgagcgccg ccgacgcccc gcgaaccgga
      841 tcccgccagt accacatcgc cctcgagcgg ggcgaggtgg cggagtacat cgtgctggtc
      901 ggcgatcccg gccgggtggc gaaggtggcg tcccgcttcg accgggtgga cctcgagcgc
      961 agcaaccgcg agatcacgac cacgaccggc gtctttcagg gcatgcccat ctcggccatg
     1021 tccaccggca tgggtcccga caacgtcgag atcctggtca gcgaggtcct cgagatcacc
     1081 gaccagccga ccttcgtccg gatcggatcg agcggggcgc tccagcccag catcggtctg
     1141 ggcgacctca tcatctcgac cggcgcggtc cggctggaga acacgaccga cttctacgtc
     1201 caccccggct accctgctat cgcccaccgc gacgtggtgt gggccctcga ggcggcgtgc
     1261 caggcggcgg ggtacgccca ccacctgggg atcacggcca ccgcgcccgg ctttttcgct
     1321 ccgcagggaa gggcgatgcg cacgctcccg gttcgctatc ctgatctcac cgaggagctc
     1381 cgacgtcaag gcgtcgtgaa cctcgaaatg gagtcgtcgg cactgtttgt gctggcgggc
     1441 ctggccggtt cgcgtgcagg aaccatctgc gccgcgtacg cgcagcgaac cgacgggagc
     1501 ttcatcgaag gcgcggacaa ggaggccgcg gaggctcgct gcattgatgc cggcctggct
     1561 ggcatccacc gcctgtgggg gaaggatcag ggcggcgggt cgtaaccaag aatcaaacgc
     1621 ttaggaggca gacggaaccg atggcgaccg acggctactg cgtcaagtgc aagaccaagc
     1681 ggaccatgaa agatgaccgc aagatcacga tgaagaacgg caagcccgcg acgcagggca
     1741 tctgccccgt gtgcggcacc aagatgttca agatcggagg ctgaggctcc gtcacgcctc
     1801 actggccgcc gagccccgct cggcggcctc tccgtttccg gcctatagaa tggaactcgt
     1861 gctagccgag gatcgcgcgc cggtcctggc catcgatctc ggcggcacgc tgatccgggt
     1921 cgcacacctg ggtcccgacc tgaccgttgc ccaccgcgag gtgatcccga ccatgccgac
     1981 cgtgggcgtc gagggagtcc tcgagcgcat gatctcgacc gcccgctcgg tcctggcggc
     2041 tgccgaggcc gccggtctgc ccgctcccgc ggcggtcggg gtggcgtcgc ccggccccct
     2101 cgatccgcag cgtggcgtcg tccaccagcc cccgaacctt cccggctggg acgaggtccc
     2161 gctcgctcac cg
//
LOCUS       DASYGU010000238         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1529156, whole genome shotgun sequence.
ACCESSION   DASYGU010000238 DASYGU010000000
VERSION     DASYGU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529156"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGO32_06715"
     CDS             <1..406
                     /locus_tag="VGO32_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV7200477.1"
                     /translation="PFGGFLDSTLDRTGEAVVYIGIVAFWLSETSSPFVPVMLAVLAL
                     SGSFLVSYSSARAEAAGFTASNGLAPRTERLVILVLGLALGGLRVPLALPLAMGLIAI
                     LAWATVVQRIWFVRKQAATAAEPTLISPDKEN"
     gene            412..1641
                     /locus_tag="VGO32_06720"
     CDS             412..1641
                     /locus_tag="VGO32_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEV7200478.1"
                     /translation="MATKIPTTPAAAVKASTNGRNGKRRADGKVRVAIVGVGNCASSL
                     VQGRYYYENARQGDNIPGLMHVDLGGYHIRDIEFVAAFDVDKNKVGKDLSEAIYAAPN
                     NTYVFQQVPHIGVTVDRGMTLDGLGKYLSEIIEKSPDETADVVGILKERDVDVVVSYL
                     PVGSEDATKWYVEQALQAGVAFVNAIPVFIGREPYWQRRFAEAGLPVIGDDIKSQVGA
                     TITHRVLTRLFMDRGVQVDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMLDY
                     DMSPDDVHVGPSDYVPWLKDRKWCHIRMEGTTYGDVPLNLELKLEVWDSPNSAGVITD
                     AIRCAKLGLDRGLAGTLVAPSSYFMKSPPLQIHDDVAHERVEAFIRGEDNETLVGTEV
                     KTPSKRRAAKAKAFARR"
     gene            1652..2656
                     /locus_tag="VGO32_06725"
     CDS             1652..2656
                     /locus_tag="VGO32_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7200479.1"
                     /translation="MAVGQTVTGSPRQRSARQVERGRERVTYWAYRTAEKALSTLPRG
                     LVVPGAVAVSNGAYDLAGAKGALVRANMAQALGLPVDDPRVSRAARHAFQNFGRYLIE
                     VMRLPVLDPADARQRVTFVGWEDLAAAQGTEGKGVLICAVHVGAMDLLAPAVMVEGEK
                     LSVVGDDTTYGRLYDHLAAIRGVHGVDVIGWRNLRRLYKVLQDGGNLVLLCDGPYRPG
                     DVPVEFLGAPTTLPAGPAVLSARTGAAILPIGARRTPDDRLEAWGLPVIRATSTEPAE
                     IYRATQALADALGSVIAADPGQWYMFRPVWPQTDAERAAAAAALARARAGEDWSAPAG
                     "
     gene            2661..3578
                     /locus_tag="VGO32_06730"
     CDS             2661..3578
                     /locus_tag="VGO32_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200480.1"
                     /translation="MSYGPRDALVRAGLELGDAFVGILPTGAAYGLADLLGSAWFRAA
                     TGRRRLVAANLARVNEAIGRPLEGAAARDQVRAAFVAHARYYLEVARLPRHAHRHRDN
                     SLIWEGGPDLAALLDKGGIVGVSAHYGNFEPAAVWMQEHGLRWVTPVEAIEPRALFDY
                     LRSRRGAGSASGELVAAPGAARRVLEGLRHHEMVAIAADRDVGTATVEVEFFGHPARM
                     PSGPATLALLTGSPVVIITLRRTGHGQFVAWSELIPWSPTGDRPADIQTLTQRITEAL
                     ARHISEAPDQWWGSFQPVWDDLTPGTVQA"
BASE COUNT          543 a         1284 c         1266 g          529 t
ORIGIN      
        1 cccgttcggc gggtttctgg acagcaccct ggaccgcacc ggcgaggccg tcgtctacat
       61 cgggatcgtg gcgttctggc tgagcgagac ctcgtcgccg ttcgtcccgg tcatgctggc
      121 tgtgctggcc ctcagcggct cgttcctggt cagctactcg agcgcacgag ccgaggcagc
      181 cggcttcacg gcctccaacg ggctggcgcc tcgcaccgag cgcctcgtga ttctcgtctt
      241 ggggctggcc ctgggcggcc tccgtgtccc ccttgccctt cccctggcga tgggactcat
      301 cgccatcctc gcctgggcga cggtcgtcca gcgcatctgg ttcgtacgca agcaggcggc
      361 aacggccgcc gagcccaccc tcatcagtcc tgacaaggag aactgaccaa catggccacc
      421 aagatcccaa ctaccccggc ggcggccgtg aaggccagca ccaacgggcg caacggcaag
      481 cggcgcgcgg acggaaaggt ccgggtcgcg atcgtcggcg tgggcaactg cgcctcaagc
      541 ctcgtccagg ggcgctacta ctacgagaac gcgcgccagg gggacaacat ccccggcctc
      601 atgcacgtcg acctgggcgg ctatcacatc cgcgacatcg agttcgtggc cgccttcgac
      661 gtggacaaga acaaggtcgg caaggacctg tcggaggcga tttacgccgc cccgaacaac
      721 acgtacgtct tccagcaggt gccccacatc ggggtgaccg ttgaccgcgg gatgaccctc
      781 gacggactgg gcaaatacct gtccgagatc atcgagaagt cgcccgacga gaccgccgac
      841 gtggtcggca tcctcaagga acgagacgtc gatgtcgtgg tctcctacct gccggtcggc
      901 agcgaggacg cgaccaagtg gtacgtggag caggcgctcc aggccggcgt cgcgttcgtg
      961 aacgcgatcc cggtgttcat cggtcgcgag ccgtactggc agcgccgatt cgccgaggcc
     1021 gggctgccgg tgatcggtga cgacatcaaa tcccaggtgg gcgccacgat cacgcaccgg
     1081 gtcctgaccc ggctgttcat ggatcgcggg gtgcaggtcg accgcaccta ccagctgaac
     1141 ttcggaggca acacggactt cctgaacatg ctcgagcgtg agcggctgga gtccaagaag
     1201 atcagcaaga cgaacgcggt gacctcgatg ctcgactacg acatgagccc ggatgacgtc
     1261 cacgtcggcc cgagcgacta cgtgccgtgg ctcaaggacc gcaagtggtg ccacatccgg
     1321 atggagggca cgacctacgg cgacgtcccg ctcaatctcg agctcaagct cgaggtctgg
     1381 gactctccga acagcgccgg cgtcatcacc gatgccatcc gctgcgccaa gctgggcctc
     1441 gaccgcggac tggccggcac cctcgtcgcc ccgtcgtcgt acttcatgaa gtccccgccg
     1501 ctccagatcc acgacgacgt ggctcacgag cgggtcgagg cgttcatccg gggcgaggac
     1561 aacgagacgc tggtcggcac cgaggtgaag accccgtcga agcggcgcgc cgcgaaagcg
     1621 aaggcgttcg cgcgtcgcta ggctgccggg agtggcagtc gggcagaccg tgaccggatc
     1681 tccgcggcag cgttccgccc gccaggtcga gcgcggccgt gagcgggtca cgtactgggc
     1741 ctaccgcacc gcggagaagg cgttgagcac gctgccgcgc gggctcgtgg ttcccggcgc
     1801 ggtggcggtc agcaacggcg cctacgacct ggccggggcc aagggcgcgc tggtgcgggc
     1861 caacatggcc caggccctgg gtctgccggt cgacgacccc cgtgtctcgc gggcggcgcg
     1921 gcatgccttc cagaacttcg gccgctacct gatcgaggtg atgcggctgc ccgtcctgga
     1981 cccggccgat gcccgccagc gggtgacgtt cgtgggctgg gaggacctgg ccgcggccca
     2041 ggggacggag ggcaagggcg tgctcatctg tgccgtccat gtcggcgcga tggacctact
     2101 cgccccggcc gtgatggtcg aaggcgagaa gctgagtgtc gtcggcgacg acacgaccta
     2161 tggccgcctc tacgaccacc tggccgcgat ccgcggcgtc cacggcgtgg atgtcattgg
     2221 ctggcggaac ctccgccgcc tgtacaaggt gctccaggac ggcggcaacc tcgtcctgct
     2281 ctgcgacggc ccctaccgac ccggcgacgt cccggtcgag ttcttgggcg cgcccaccac
     2341 gctcccggcg ggaccggcgg tgctgtcggc gcgcaccggg gccgccatcc tgcccatcgg
     2401 cgcccgtcgc acgcccgacg accggctcga ggcctggggg ctgcccgtca tccgggccac
     2461 gagcacagag ccggccgaga tctaccgagc cacccaggcc ctggccgatg ccctgggctc
     2521 ggtcatcgcc gccgatccgg gccagtggta catgttccgt cccgtctggc cgcagaccga
     2581 cgccgagcgc gcggctgccg cggccgccct ggcgcgggcc cgcgccggcg aggactggtc
     2641 cgcgcccgcc ggatgagccg atgagctacg ggccgcggga cgcgctggtc cgcgcgggcc
     2701 tggagctggg cgatgcgttc gtcggcatcc tgcccaccgg cgcggcgtat ggcctcgccg
     2761 acctcctggg cagcgcatgg tttcgtgccg ccaccgggcg gcggcggctg gtggcggcca
     2821 atctcgcgcg cgtgaacgag gcgatcggcc gcccgttgga gggcgccgcc gcccgcgacc
     2881 aggtgcgcgc cgcgttcgtg gcccacgccc gctactacct cgaggtcgcg cgtctccccc
     2941 gccacgccca tcggcatcgg gacaactcgt tgatctggga gggcgggccc gatctggcgg
     3001 ccctgctcga caagggcggc atcgtgggcg tatcggccca ctacggaaac ttcgagcccg
     3061 cggcggtgtg gatgcaggag cacggtctgc ggtgggtcac cccggtcgag gcgatcgagc
     3121 ccagggcgct gttcgactac ctccgctcgc gtcggggagc gggcagcgcg agtggcgaat
     3181 tggtcgccgc tccgggggcc gcgcggcgcg tgctcgaggg cctccgccac cacgagatgg
     3241 tggccatcgc cgccgaccgt gacgtgggca cggctacggt agaagtggag ttctttggcc
     3301 acccggcgcg catgccctcc ggacccgcga cgctcgcgct gctgaccggg tcgccggtcg
     3361 tgatcatcac cctgcgccgg acgggccacg ggcagttcgt ggcctggagc gagctgatcc
     3421 cgtggtcgcc gaccggcgac cggccggccg acatccagac cctgacccag cgcatcaccg
     3481 aagcgctggc ccgtcatatc agcgaggcac cggaccagtg gtggggatcg tttcagccgg
     3541 tgtgggacga cctgaccccc gggacggtcc aggcctgatg gaccgatccc cctcggctgg
     3601 agaccgcggc aaggccgacc tc
//
LOCUS       DASYGU010000239         7015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_192049, whole genome shotgun sequence.
ACCESSION   DASYGU010000239 DASYGU010000000
VERSION     DASYGU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192049"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGO32_06735"
     CDS             <1..241
                     /locus_tag="VGO32_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV7200481.1"
                     /translation="AVLASADLVLGAVDDEFSDRPEGTVIRSNPAAGTTVDVGRQVDI
                     VISRGPEPTPTPVPTPPPTPTPPPTPTPPPASPAP"
     gene            319..639
                     /gene="trxA"
                     /locus_tag="VGO32_06740"
     CDS             319..639
                     /gene="trxA"
                     /locus_tag="VGO32_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV7200482.1"
                     /translation="MSAVAVDETTFENEVLNADKPVLVDFWAPWCGPCRLVSPVVESI
                     GDAHAEKIAVAKLNTDENQDLAMRYSIFSIPTLILFKDGREAARLVGYMPQPVIEERL
                     RNWL"
     gene            650..1243
                     /locus_tag="VGO32_06745"
     CDS             650..1243
                     /locus_tag="VGO32_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200483.1"
                     /translation="MEGRPPPRQIVIKPERRAGSERRQAEAPAPADRRESDRRTMCWE
                     VENCDLAIRYRCPAFILRRPCWELWAVEGLGPARACCHNDTDCEPGRCAIAEKRFAAT
                     AATAEPLAVHVGRRGAPLGFTKPRRHSCPNFFVTHGSGGRIPVDAPHLLRSLAKQEGT
                     VSRCRVRGGVHLDSGYVTDLCLTGQYRDCVFFEDEQD"
     gene            1270..2595
                     /locus_tag="VGO32_06750"
     CDS             1270..2595
                     /locus_tag="VGO32_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV7200484.1"
                     /translation="MAAIRQRGRFGVNLGLDRIERLLAGLGHPERELHGALVAGTNGK
                     GSVVALAGAVLRHAGSRVGAMPKPHLVTYRERITIDGAMLAPDAFAAIVGEVIAEADR
                     ETGAEGPPTEFEILTAAGLLALAQARVDLALVEVGLGGRLDATNASDLGVAAITTVAH
                     DHERHLGRTLAAIGSEKAAIIKPGNLAVTGASGRGLVPVMERCAALDAPLRRSGRGQP
                     WEARVVDSGWQGVTVDLRTPTGTVRDLRIGLLGSHQAANAATAVALLDAIREDGERRG
                     QPVVVDEPALRAGMADARWPGRLELFPSAAGGREVLLDGAHNPSGARALARALGELGV
                     RNPPLVFGAMRGKRVAAVLRALASLAPRPVFTAVADPHAHRPDELLRTWRRVAPATRG
                     AEAGTTTSSPRDALGRAAALGGRGPVVVAGSLYLVGAVRAELTGEREDG"
     gene            2595..3854
                     /gene="folP"
                     /locus_tag="VGO32_06755"
     CDS             2595..3854
                     /gene="folP"
                     /locus_tag="VGO32_06755"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV7200485.1"
                     /translation="MRTLTFGGRTHVMGILNVTPDSFSGDGLAPDGRAEHEVVAAALE
                     QAQAFVAAGADILDIGAESSRPAAVYGAHPALDVESELRLVLPVVRAVVAELGSRAMV
                     SIDTTKGAVARAALDAGAEMVNDVWAARRDPDTAAAAAEAGAYLVVMHNKDVAEYPHG
                     VLPEVIAWLRDSAEAAAAAGVVADRILVDPGIGFGKTPDHSLEVLHRLAELRAALGLP
                     MLVGTSRKRFIGEILDGAPPDQRLEGTAASVVLAVAAGADVVRVHDVEPVTKAVRVAD
                     AIVRYRAVHQRTADPDVPGRISVRGIAAAGRHGVGEEERSRPQPFEVDVVASLDAGPA
                     AAGDRLEDTVDYAALSGQVVERVGGTSYHLIETLAADIGQATLDRWPMVSAVEVTVRK
                     PEASMPVPVTGVEVRIQIARERRAGDA"
     gene            complement(3868..5136)
                     /locus_tag="VGO32_06760"
     CDS             complement(3868..5136)
                     /locus_tag="VGO32_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7200486.1"
                     /translation="MTDNEFEVEPPSAVETDRYGASEPTAPIPSRSDNWSSAPAWSTR
                     RSLPAEPPEAPPARRPRMLSFVAVALAAGVVAGSLSGLAVVNLMGVVETPATEVPPGD
                     TVSAVTLDESSAVIESVASVAPAVVTINVTLATGGSGSGSGFIFDPDGWILTNRHVAG
                     DAATLVVTLADSRRFDATLVGVDTLTDLAIIKIDATDLPTAPIGSSAALEIGQLTIAI
                     GNPLGDYADTVTTGVVSGLGRAIQAGDGSSLENISHLIQTDAAINPGNSGGPLVNSLG
                     QVIGINTAIATSAEGIGFAIPIDFAKPIMALALDGEEIARPWVGVYYTMIDQQLATDR
                     DLSVDEGALIDEGPNGAPGVFAGSPADAAGLQQGDIITAIDGRPVDSAHDLVVLLLPH
                     RAGDVVDATVVRDGEELQIAITLGTLPAAP"
     gene            complement(5198..6727)
                     /locus_tag="VGO32_06765"
     CDS             complement(5198..6727)
                     /locus_tag="VGO32_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7200487.1"
                     /translation="MVALASTALIALLISGVALNQILPGYFEEQGRARTESAAQATAL
                     LIIDTANRSPLREFLTDPALRETRIIQPAAQEASDGLLVTVEVFNTVDGSQAAFAEPQ
                     DVEAAEAQDLAPDPLAPATRLTFAIELPLSEEPLAPDGVLNMQVLVSVPYTAREATLA
                     QVRGALVGTGTLALILSMLVGVLAARRLTVPLSRLGSFSGRLAHGHLDARVPPTGVVE
                     IDQLGDQFNIMADRLSESLRLLESDRDRLREFVADVSHELRTPLAALRTYNELERDGD
                     MDEATRREFLERSGEQLSRLEWMSANLLDLSRIDAGIFPLDVRVGDLREPVRAAVEAT
                     AETAAARGVSVTYAAPSTSVAIRFDRERIVQLVNNLVGNAVKFTPSGGTVAVQVLDEA
                     DAAAIAVSDTGPGIPPAELPRIFERFYRGTNVGEARASGSGLGLAIARSIVEMHGGFI
                     EVASVLGEGAVFRVVLPRQGAAAAGERAGDAPIPDWTAPVPAAPDGGLTDDLAPEETP
                     A"
     gene            complement(6774..>7015)
                     /locus_tag="VGO32_06770"
     CDS             complement(6774..>7015)
                     /locus_tag="VGO32_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7200488.1"
                     /translation="ISLTTTEFDLLRHLAAKPGRVFSRSQLLELVRDYDAAVEQDERT
                     INVHVSHIRDKIEPDPAQPRYIRTVRGVGYAFAED"
BASE COUNT          997 a         2449 c         2600 g          969 t
ORIGIN      
        1 ggcggtactc gcgtcagccg acctggtgct gggcgccgtg gacgacgagt tctctgatcg
       61 accggagggg acggtcatcc gttccaatcc cgccgcagga accaccgtcg acgtcggccg
      121 acaggttgac atcgtcatct cccgcggccc ggagcccacg ccgaccccgg tcccgacccc
      181 gccgccgacg ccgaccccgc cgcccacgcc aacaccgccg ccggcctcgc ccgcgcccta
      241 gccctagccg ataggtacct tcggcggtgc taggatggcc gccgcttacc gtccgccgca
      301 cctgaggtca ctgtgcccat gtccgctgtc gccgtcgatg agacgacctt cgagaacgag
      361 gtcctcaacg ccgacaaacc cgtcctggtc gacttctggg caccgtggtg tggcccctgc
      421 cggctggtct cccccgtcgt cgagtccatc ggcgacgccc acgccgagaa gatcgcggtc
      481 gccaagctga acaccgatga gaaccaggac ctggccatgc ggtacagcat cttcagcatc
      541 cccaccctga tcctgttcaa ggacggtcgc gaagcggctc gcctggtcgg ctacatgccg
      601 cagccggtca tcgaggagcg cctcaggaac tggctctagg cgtctgccga tggagggacg
      661 gccgccaccc cgccagatcg tcatcaagcc cgagcgccgg gcgggatccg aacgtcgcca
      721 ggccgaagcc ccggcacccg ccgaccgtcg cgagagcgac cggcgaacga tgtgctggga
      781 ggtcgagaac tgcgacctcg ccattcgcta ccgatgcccg gcgttcatcc tccgccgccc
      841 gtgctgggag ttgtgggcgg tcgagggcct gggcccagcg cgggcctgct gccacaacga
      901 caccgactgc gagccgggac ggtgcgcgat cgccgagaaa cggttcgcgg ccacagcggc
      961 caccgcggag ccattggccg tccatgtcgg tcgtcgcggt gccccgctcg gcttcaccaa
     1021 gccccggcgc cacagctgcc cgaacttctt cgtgacccac ggatcgggcg gccggattcc
     1081 ggtcgacgca ccgcacctgc tccgctcgct ggccaagcag gaaggcaccg tctcccgctg
     1141 ccgggtgcgg ggcggcgtgc atctcgacag cggctacgtc accgacctgt gcctgaccgg
     1201 ccagtaccgg gactgcgtct tcttcgagga cgagcaggac tgagcaccgc cctcacctac
     1261 gccgacgcgg tggcggccat ccgccaacgg ggccgattcg gagtgaacct gggcctcgac
     1321 cggatcgagc gcctcctggc gggccttggc cacccggagc gcgagctgca cggagccctg
     1381 gtggccggga ccaacgggaa gggcagtgtg gtggcactcg ccggcgcggt cctccggcac
     1441 gcgggctcgc gtgtcggggc gatgcccaag ccccatctcg taacgtaccg cgagcgcatc
     1501 acgatcgacg gcgcaatgct cgcgcccgat gcgttcgcgg caatcgttgg cgaggtgatc
     1561 gcggaggcgg accgcgagac gggggctgag gggccgccta cggagttcga gatcctgacc
     1621 gccgccggtc tgctggcgct cgcccaggcg cgggtggacc tggcgctggt cgaggtcggg
     1681 ctcggcggac ggctggatgc gaccaacgca tcggacctcg gcgtggccgc catcacgacc
     1741 gtggcccatg atcacgaacg ccacctgggc cggacgttgg cggccatcgg tagcgagaaa
     1801 gcggcgatca tcaagcccgg caacctggcg gtaaccggcg ccagcgggcg cgggctggtg
     1861 ccggtcatgg agcgatgcgc ggcgctcgac gcaccgctgc ggagaagcgg gcgcgggcaa
     1921 ccatgggagg cacgggtggt ggacagcggc tggcaggggg tcaccgtgga cctccgcacg
     1981 ccgacgggaa ccgttcggga tctccggatc ggtttgctgg gaagccacca ggccgcgaac
     2041 gcggccaccg ccgtcgccct cctcgacgcg atccgcgagg acggggagcg gcggggccag
     2101 ccggtggtcg tggacgagcc ggcactccgc gccgggatgg ccgacgcgcg ctggcccggc
     2161 cgcctggagc tgttcccgtc cgcggccggc ggtcgcgagg tgctcctgga tggcgctcac
     2221 aacccgtccg gcgcacgggc cctggcccga gccctcggcg agctgggggt gcggaaccct
     2281 ccgctcgtgt tcggcgccat gcgcggcaag cgcgtggccg ccgtcctcag ggctctcgcc
     2341 tcgttggctc cgcgaccggt gttcaccgcg gtcgccgatc cgcacgcgca tcggccggac
     2401 gagctgctcc ggacgtggcg ccgggtcgcg ccggcgacgc ggggcgcgga ggccgggacg
     2461 acgacctcat ccccgcgcga cgcgctgggc cgcgccgcag cgctgggtgg ccgcgggccg
     2521 gtggtggtcg ccgggtcgct gtacctggtc ggggcggtcc gcgccgagct gaccggcgag
     2581 cgcgaggacg gctgatgcgc accctcacct tcggcgggcg gacccacgtg atgggcatcc
     2641 tcaacgtgac ccccgactcg ttctcgggcg acgggctcgc tcctgacggc cgggcggagc
     2701 acgaggtggt ggccgccgcc ctggagcagg cacaagcgtt cgtggcggcc ggcgccgaca
     2761 tcctggacat cggcgctgag tcgtcgcgac ccgcggcggt ctacggcgcg catccggcgc
     2821 tcgacgtcga gtcggagctg cggctcgtcc tgcccgtggt cagggcggta gtcgcggagc
     2881 tcgggtcgcg ggcgatggtc agcatcgaca ccaccaaggg cgcggtggcc cgcgcggccc
     2941 tggacgccgg ggcagagatg gtcaacgacg tgtgggccgc ccggcgcgac cccgacaccg
     3001 cggccgctgc cgccgaggcc ggcgcctacc tcgtcgtcat gcacaacaag gacgtggccg
     3061 agtaccccca cggcgtgctg ccggaggtca tcgcgtggct ccgggacagc gcggaggcgg
     3121 cggcggcggc gggcgtggtc gccgatcgga tcctagtcga ccccggcatc ggctttggca
     3181 agacgcccga ccacagcctc gaggtgctcc atcggttggc cgagctgcgc gccgcgctgg
     3241 gcctgcctat gctggtgggc accagccgca agcgattcat cggcgagatc ctggatggcg
     3301 cgccgcccga tcagcggctg gagggaaccg ccgcgagcgt ggtgctggcg gtcgcggccg
     3361 gtgcggacgt ggtgcgggtc catgacgtcg agcccgtcac gaaggccgtc cgcgtagccg
     3421 acgccatcgt ccggtaccga gccgtccacc agcggacggc ggacccggac gtcccgggcc
     3481 ggatctcggt tcgaggcatc gcggcggccg gacgccacgg cgtgggcgag gaagagcggt
     3541 cgcggcccca accgttcgag gtcgacgtcg tcgcatccct ggacgcgggg cccgcggcgg
     3601 ccggcgatcg gctcgaggac accgtggact acgcggcgct cagcggacag gtggtcgagc
     3661 gggtcggggg caccagctac cacctgatcg agaccctggc ggcggacatc gggcaggcga
     3721 ccctcgaccg gtggccgatg gtcagcgcgg tcgaggtcac ggtgcgcaag cccgaggcct
     3781 cgatgccggt gccggtcacc ggggtcgagg ttcggatcca gatcgcgagg gagcgccggg
     3841 cgggagacgc ctgacggcgt ccgcccctca cggagcggcg ggcagggtcc cgagcgtgat
     3901 cgcgatctgg agctcctcgc catcccggac gacggtggcg tcaacgacgt cgccggcgcg
     3961 gtgcgggagg agcaggacca cgaggtcatg ggccgaatcg accgggcgac cgtcgatcgc
     4021 ggtgatgatg tcgccctgct ggaggccggc cgcatcggcc gggctgccgg cgaagacgcc
     4081 cggcgccccg ttcgggccct cgtcgatcag cgcaccctca tcgacggaga gatcgcggtc
     4141 ggtggccagc tgctggtcga tcatcgtgta gtacacgccg acccacggtc gggcgatctc
     4201 ctcgccatcg agcgccagcg ccatgatcgg cttcgcgaag tcgatcggga tcgcgaaccc
     4261 gatgccctcg gcactggtcg cgatcgcggt gttgatcccg atcacctggc ccaggctgtt
     4321 caccagcggg ccgcccgagt tgcccgggtt gatggccgca tcggtctgga tgaggtggct
     4381 gatgttctcg aggctcgacc catcgccggc ctggatcgcg cggcccagtc cggagaccac
     4441 gccggtcgtc accgtgtcgg cgtagtcgcc caggggatta ccgatcgcga tcgtgagctg
     4501 accgatctcg agcgccgccg atgagccgat gggtgcggtc ggcaggtcgg tggcgtcgat
     4561 cttgatgatc gccaggtcgg tcagggtgtc gaccccgacc agggtcgcat cgaaacggcg
     4621 ggagtcggcg agggtgacga ccagcgtggc cgcgtcaccg gccacgtggc ggttggtgag
     4681 gatccagccg tcggggtcga agatgaagcc cgatcccgag ccggagccgc cggtcgcgag
     4741 cgtgacgttg atggtgacca cggccggcgc gacgctggcc accgattcga tgacggccga
     4801 cgactcgtcg agggtcaccg ccgagaccgt gtcgccgggt gggacctcgg tcgcgggcgt
     4861 ctcgaccacg cccatcaggt tgacgacggc cagcccggac agggagccgg caacgacgcc
     4921 ggcagcgagc gcaacggcga cgaagctgag catcctgggt cggcgggcgg gcggcgcctc
     4981 gggtggctcg gccggcagcg agcggcgcgt cgaccatgcg ggggccgagg accagttgtc
     5041 ggatcgggaa gggatcggcg cggtgggttc gctggcgccg tagcggtcgg tctcgaccgc
     5101 cgacggcggc tcaacctcga attcgttgtc agtcacgctc gcagtgtgcc gcgcggcgcg
     5161 ttaggcccca accccggcga cggtgaagat tcgttgatca agccggggtc tcctcgggcg
     5221 ccagatcatc ggtcagtccc ccatctggtg ccgcgggcac gggtgccgtc cagtccggga
     5281 tcggtgcatc gccggcacgc tcaccggcgg ccgcggcgcc ctgccgtggg agcacgactc
     5341 ggaacaccgc gccctcgccc agcacgctgg ccacctcgat gaagccgcca tgcatctcga
     5401 cgatcgagcg agcgatggcc aggccgagcc cactccccga agcgcgtgcc tcgcccacgt
     5461 tcgtcccccg gtagaagcgc tcgaagatgc gcggcagctc ggccggcgga atgccgggcc
     5521 cggtgtcgct caccgcgatt gccgcggcat cggcctcgtc caggacctgg accgccaccg
     5581 tcccgccgga cggggtgaac ttcacggcat tgcccaccag gttgttgacc agctgcacga
     5641 tccgctcgcg gtcgaagcga atggcgaccg aggtggaggg ggcggcatag gtgacgctga
     5701 cgccacgcgc cgcggccgtc tcggccgtgg cctcgaccgc ggcgcggacc ggctcgcgga
     5761 ggtcgccgac gcggacgtcc agcggaaaga tgccggcgtc gattcgcgac aggtcgagca
     5821 ggttggcgct catccattcg agccggctga gctgctcccc ggaccgctcg aggaactccc
     5881 gacgcgtggc ctcgtccatg tcgccgtcgc gttcgagctc gttatacgtg cgcagtgcgg
     5941 cgagcggcgt tcgcagctcg tggctgacat cggcgacgaa ttcgcgcagg cggtcacggt
     6001 ccgactcgag caggcggagc gactcgctca ggcggtcggc catgatgttg aactggtccc
     6061 ccagctggtc gatctccacc acgccggtcg ggggcacgcg ggcgtcgaga tggccgtgag
     6121 ccaggcgacc cgagaagctc cccagccggc tcaacgggac ggtcagccga cgggcggcga
     6181 ggaccccgac cagcatggag aggatcagcg cgagcgtccc ggtcccgacc agcgcccctc
     6241 ggacctgggc cagggtcgct tcccgggcgg tgtacggaac cgaaaccagg acctgcatgt
     6301 tcagcacgcc gtccggcgcg aggggttcct cgctgagcgg gagctcgatc gcgaacgtca
     6361 gccgggtggc cggtgccagc ggatcggggg cgaggtcctg ggcctccgcc gcctcgacgt
     6421 cctgtggttc ggcgaacgcg gcctgcgagc cgtccaccgt gttgaacacc tcgaccgtga
     6481 ccagcagccc gtccgaggcc tcctgcgccg cgggttggat gatccgggtc tcgcgcagtg
     6541 ccgggtcggt caggaactcg cgcagcggag accgattggc ggtgtcgatg atgagcagcg
     6601 cggtcgcctg ggcggccgat tcggtacggg cccggccctg ctcctcgaag tagccgggca
     6661 ggatctggtt cagggccacc ccgctgatga ggagcgcgat gagcgccgtg ctggccagcg
     6721 cgaccaccag gcgcacgtcc aggcgacgga ggaagcgtcg gacgaacgtg ggctcagtcc
     6781 tcggcgaacg cgtagccgac gccgcggacg gtccggatgt accggggctg ggccggatcg
     6841 ggctcgatct tgtccctgat atggctgacg tggacgttga tcgtccgctc gtcctgctcg
     6901 accgcggcgt cgtagtcgcg gacgagctcc aggagctggc tgcggctgaa gacccgcccg
     6961 ggcttggccg cgaggtggcg gaggaggtcg aactcggtcg tggtcagact gatcc
//
LOCUS       DASYGU010000240         4857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1323981, whole genome shotgun sequence.
ACCESSION   DASYGU010000240 DASYGU010000000
VERSION     DASYGU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323981"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1242
                     /gene="dnaN"
                     /locus_tag="VGO32_06775"
     CDS             106..1242
                     /gene="dnaN"
                     /locus_tag="VGO32_06775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV7200489.1"
                     /translation="MNISVMQENLARGLGIVSRAVANRATLPVLANVLLRTEDGGLKL
                     TATNLEIGITCWVPGKVEDGGEITVPARLLTDLVGSLPPQKIDLELSAKDRTLKVTCG
                     GSRASIKGIEADEFPVVGGIGEAPITSVDARVLREALGEVVFAAATDESRPILTGVLT
                     RFEGDTMTLAAADNYRIAVRTLTLSKPVAAETTIVVPGRSYAELVRILPDAEAPVEIT
                     ITPNRSQVLFHVEGIDLVSRLIEGQFPNYEPIVPRKDAWTARATVDREAFLAGTRRAS
                     FFARDSANIIKVEVGGENGDASTVTISAHAVDIGDNADSLEATVEGSPTTIAFNARYL
                     TDVLGALSAEEAILELSGPLAPGVIRGVGKDEYVHVIMPVRTAS"
     gene            1250..2431
                     /locus_tag="VGO32_06780"
     CDS             1250..2431
                     /locus_tag="VGO32_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV7200490.1"
                     /translation="MRLARLQLDHFRSYTAAELVPDPGLTLIAGPNGAGKTNLLESIW
                     VTVSGRSHRTATDAELVSHGAPLARVTLDLTGDDGPATLELVLPGVEPTPGVRRRLSL
                     NGIARRQTSVADTLRAVLFRPEEMLLLVGAPSERRRFLDAILAQRSRATARDLADYAR
                     VLAQRNALLRAIRAEEADPAVLPVWDEQLAIVGGRVLAARTALVDDLAARIGPLHDAV
                     APADERGDRVEVAYLDSLKDAWPERDAPTRDPSRLDPDRLATAIRRRLEEVRQKDIWN
                     GMSLAGPHRDDIGVTLGGVPVAAHASRGQQRTIIVALKLAETDLLAEDDGPAPIVLLD
                     DVFSELDPERSERGLDLLRQRGQVLVTITDPGLLPARHRHRVPVWTVGDGQLALAPRV
                     A"
     gene            2442..2795
                     /locus_tag="VGO32_06785"
     CDS             2442..2795
                     /locus_tag="VGO32_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200491.1"
                     /translation="MAGRQTSEEAASAVQRALLQCVGPGAAEQLALAQARLAWLEAVG
                     DAGLAPEGMWSRVITVSNGVAAVVASEPMLAAELKLRGEQLVRAVNDRQRGRPGALYE
                     LRTITVSVRPGATPP"
     gene            2792..>4857
                     /locus_tag="VGO32_06790"
     CDS             2792..>4857
                     /locus_tag="VGO32_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200492.1"
                     /translation="MSQRLAAKVGIVSDADRGPDARDLVRLNEPTVGAPVRTKGSLYL
                     VAQLTDPGSGTVRAARQALEAIEHDYYYDLSAGPIDTLSRALAQANRRLFHARGRAGL
                     NRGAQISVVAAAIRGAELHVAKIGPAGAIIVRGDRIFELPPPGAAEDDIDSDRRVADS
                     LGEVLEVEPVTWSGELGHGDRVALISRNLAEIVGAEDTRKTLLELRPGAAARHLHELF
                     LERGGSGSDGLLTIELNAIALTATTHRLEPVRPADPLAGLPDRSPVPGADAVGGALRR
                     ADHAVDHAQSRVGSSLDVWIGRIVTLLPRRRVDYPRRVVRTEALEARRRRRVGALGML
                     ALAGLLAAAVTVASDSSIDPTQAIPRLEVAREAIAEGESLIAEVESRVDGQDLVERDP
                     DRAKDLLAEADRALIRAEGAGVPAASLEPLRVRLDGRRDALYTVSRIRELTTVVDLAA
                     TFTGVDASRMVAASDGSLWILETGRGRVIRVDPLTGASEAVYRAGQQLDDGTGGDPWL
                     IATAATDVVVVDRDRQAWRADLVERVMRRMPMAGIDTLDASSQLLTALQHRPPLEIFN
                     LYAVDGAEGTVWKWTPPAVIPVTFPVAPESFLTSEPDLDPANARDLRADANLWLLQRA
                     TVTRANFGTPIPQTEFSLDPPPDAAVREALDYTLFDAATIGDRDQFFVYDAANARIVA
                     FQRADGA"
BASE COUNT          682 a         1820 c         1700 g          655 t
ORIGIN      
        1 tttccgatcg gtggggatcg atgtccacac cgtccacacc cctacgacga caggcggcga
       61 atagaaaact ctatgcttca aggaaccgta gttgagacgc agtcaatgaa catctccgtc
      121 atgcaggaga acctggcccg cgggctggga atcgtgagcc gagcggtggc caaccgcgcc
      181 acgcttcccg tcctggccaa cgtcctgctc cggaccgagg atggcgggct caagctcacc
      241 gccaccaatc tcgagatcgg gatcacgtgc tgggtgcccg gcaaggtcga ggatggcggc
      301 gagatcaccg tgccggcccg cctcctgacc gacctggtcg gatcgctgcc gccgcagaag
      361 atcgacctcg agctctcggc caaggatcgg accctgaagg tgacctgcgg cgggagccgc
      421 gcctcgatca agggcatcga ggccgacgag ttcccggtgg tcggcggcat cggcgaggcg
      481 ccgatcacgt ccgttgacgc ccgtgtcctg cgcgaagcgt tgggcgaggt cgtgttcgcg
      541 gccgccaccg acgagtcgcg cccgatcctg accggcgtcc tgacccggtt cgagggcgac
      601 acgatgaccc tggccgccgc cgacaactat cggatcgcgg tccggacgct caccctcagc
      661 aagcctgtcg cggccgagac cacgatcgtg gttcccggcc gctcgtatgc ggagctggtg
      721 cggatcctgc ccgacgccga ggcgccggtc gagatcacga tcaccccgaa ccgaagccag
      781 gtcctgttcc atgtcgaggg catcgacctc gtcagccgcc tcatcgaagg gcagttcccc
      841 aactacgagc cgatcgtccc gcgcaaagac gcctggaccg cccgcgcgac cgtcgatcgc
      901 gaggcgttcc tggccggcac ccggcgggcg agcttcttcg cccgcgactc ggcgaacatc
      961 atcaaggtgg aggtcggcgg cgagaacggg gacgcgtcga cggtcacgat cagcgcccac
     1021 gcggtggaca tcggcgacaa cgccgactcg ctggaggcca cggtcgaggg ctcgccgacc
     1081 acgatcgcgt tcaatgcccg ctacctgacc gatgtcctcg gcgccctgag cgccgaggag
     1141 gccatcctcg aactctccgg gccgctggcc cccggcgtga tccggggtgt cggcaaggac
     1201 gagtacgtcc acgtcatcat gccggtgcgg accgcctcct gaccttgatg tgcgtctcgc
     1261 gcgactccag ctcgaccact tccgaagcta cacggccgct gagcttgtcc ccgacccggg
     1321 gctgaccttg atcgccggcc cgaacggcgc cggcaagacc aacctcctcg agtcgatctg
     1381 ggtcaccgtc tccggccgct cccaccggac cgcgaccgac gccgagctcg tctcgcacgg
     1441 cgcgccattg gcccgcgtga cgctcgacct gaccggtgat gacggcccgg ccacgctcga
     1501 gctcgtgctc cccggcgtcg agcccacccc gggcgttcgc cgccgcctga gcctgaacgg
     1561 catcgcccgc cgccagacct cggtcgccga cacccttcgc gcagtcctgt tcagacccga
     1621 ggagatgctc ctcctggtcg gggcaccatc ggagcggcgg cggttcctgg acgcgatcct
     1681 cgctcagcgc tcccgggcca cggctcgcga cctggccgac tacgcacggg tcctggccca
     1741 gcgcaacgcg cttctccgcg cgattcgggc ggaggaggcc gatccggccg tccttccggt
     1801 ctgggacgag cagctggcaa tcgtgggtgg acgggtgttg gccgcccgca ccgcattggt
     1861 cgacgacctg gccgcgcgga tcgggccgct ccacgatgcg gtcgccccgg ccgacgagcg
     1921 cggcgaccgg gtggaggtcg cgtatctcga ctcgctgaag gacgcgtggc ccgaacgcga
     1981 cgctccgacc agggacccgt cacggctcga cccggaccgc ctcgccacgg ccatccggcg
     2041 gcgcctggag gaggtgcgcc agaaggacat ctggaacggg atgtcgctcg ccggcccgca
     2101 ccgagacgac atcggcgtca cgctcggcgg ggtgcccgtg gccgcgcacg ccagtcgtgg
     2161 ccagcagcgg acgatcatcg tcgccctcaa gctggccgag accgacctcc tggccgagga
     2221 cgacgggccg gcgcccatcg tcctgctgga cgacgtgttc agcgagctcg accccgagcg
     2281 atcggagcgc ggcctcgatc tcctccgtca gcgcggacag gtcctggtca cgatcacgga
     2341 cccgggcctg ctcccggccc gccaccgcca ccgggtgccg gtgtggaccg tgggcgacgg
     2401 gcagctggcg ctggcgccgc gcgtggcctg aggcggacga ggtggccggt cggcagacca
     2461 gcgaggaggc ggccagcgcg gtccagcgcg ccctcctcca gtgcgtgggc ccgggcgcgg
     2521 cagagcagct ggccctggcc caggcccggc tggcgtggct cgaggcggtt ggtgacgcgg
     2581 gcctggcccc cgagggcatg tggagccggg tcatcacggt cagcaacggg gtcgccgcgg
     2641 tcgtggcctc cgagccgatg ctggccgccg agctgaagct tcgcggcgag cagctggtcc
     2701 gggccgtgaa cgaccggcag cggggacgcc cgggcgcgct gtacgagctg cgcacgatca
     2761 ccgtctccgt ccggccaggg gcgacgccac cgtgagccag cgcctggcgg cgaaggtcgg
     2821 catcgtctcc gacgcagacc gcggacccga cgcccgagac ctggttcggc tcaacgagcc
     2881 caccgtcggg gccccggttc ggaccaaggg cagcctgtac ctcgtcgccc agctcaccga
     2941 cccgggctca ggcaccgtgc gcgccgcccg ccaggcgctc gaggcgatcg agcacgacta
     3001 ctactacgac ctgtccgccg gcccgatcga cacgctctcg cgcgcactgg cccaggccaa
     3061 ccggcgcctg ttccatgccc gcggccgtgc cggcctgaac cgtggcgcgc agatcagcgt
     3121 cgtggccgcc gcgatccgtg gcgcggagct gcacgtggcc aagatcgggc cggcgggcgc
     3181 gatcatcgtc cgcggcgacc ggatcttcga gctgcctccg cccggcgccg cggaggacga
     3241 catcgactcg gatcgccggg tggccgactc gctgggcgag gtgctcgagg tcgagccggt
     3301 gacatggtcg ggcgagttgg gccacgggga ccgggtggcg ctcatcagcc gcaacctggc
     3361 cgagatcgtc ggggccgagg acacgcgcaa gaccctcctc gagcttcggc cgggtgcggc
     3421 ggcgcggcac ctccacgagc tgttcctcga gcgtggcggc agcggttccg acggcctgct
     3481 cacgatcgag ctgaacgcca tcgcgctgac tgccaccacg catcggttgg agccggtgag
     3541 gccggccgac ccgctggccg gcctgcccga ccgcagcccc gtccccggtg ccgacgcggt
     3601 gggcggtgcc cttcgccgcg ccgaccacgc ggtcgaccat gcccagtcgc gggtcggcag
     3661 ctcgctcgac gtgtggatcg gccggatcgt caccctgctg ccccgtcggc gggtcgacta
     3721 tccccgccgc gtggtccgga ccgaggccct ggaggctcgc cgccgccgtc gggtcggcgc
     3781 gctgggcatg ctggccctgg ccgggctgct ggccgccgcg gtgaccgtcg ccagcgattc
     3841 gtccatcgac cccacccagg ccatcccgcg gctcgaggtg gcccgcgagg ccatcgccga
     3901 gggcgagtcc ctgatcgcgg aggtcgagag ccgtgtggac ggccaggacc tcgtcgagcg
     3961 ggatcccgat cgggccaagg acctcttggc cgaggccgac cgggcgctca tccgggcgga
     4021 gggcgccggc gtccccgcgg cgtcgctgga gccgctgcgc gtccggctgg atggacggcg
     4081 ggacgccctg tacaccgtct cccggatccg ggagctgacc acggtcgtgg acctggccgc
     4141 caccttcacc ggggtcgacg ccagccggat ggtggccgcc agcgacggct cgctgtggat
     4201 cctcgagacc ggccgcggcc gcgtcatccg ggtcgatccg ctgaccgggg cgtcggaggc
     4261 ggtgtaccgc gccggccagc agctggacga tggcacgggc ggcgacccgt ggctcatcgc
     4321 gaccgccgcc accgacgtcg tggtcgtcga ccgcgaccgc caggcctggc gcgccgacct
     4381 ggtcgagcgg gtgatgcgtc ggatgccgat ggcgggcatc gacaccctgg acgccagcag
     4441 ccagctgctc accgcgctcc agcaccggcc gccgctcgag atcttcaacc tgtacgcggt
     4501 cgacggcgcc gaggggacgg tgtggaaatg gacgccgccc gcggtcatcc cggtcacctt
     4561 ccccgtcgcc cccgagtcgt tcctcaccag cgagcccgac ctggacccgg ccaacgcccg
     4621 tgacctgcgg gcggacgcca acctgtggct cctccagcgg gcgaccgtca cccgcgcaaa
     4681 cttcggcacc cccatccccc agaccgagtt ctcgctcgac ccgccgcccg acgccgccgt
     4741 gcgcgaggcg ctggactaca ccctgttcga cgcggcgacc atcggcgacc gcgaccagtt
     4801 cttcgtctac gacgcggcca atgcccggat cgtcgccttc cagcgggcgg acggagc
//
LOCUS       DASYGU010000241         8723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1221808, whole genome shotgun sequence.
ACCESSION   DASYGU010000241 DASYGU010000000
VERSION     DASYGU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221808"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1086
                     /locus_tag="VGO32_06795"
     CDS             97..1086
                     /locus_tag="VGO32_06795"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEV7200493.1"
                     /translation="MAATTRPARPRAGHLVIIGGAEDKLRQRTILTRFVTLAGGPAAR
                     ILVISTASSLGDEATDLYASLFRQLGVAEVTSARPMTRDEANAPRIADELRDVTGVFL
                     TGGNQLRLSTVIGGTKLGRALVDAHKRGLVVAGTSAGASAIASHMVAFGASGATPKQR
                     MTQMSAGLGLLSGVVIDQHFEQRNRIGRLIALVAQSPSLLGIGLDEDTAAVVSPSGML
                     EVIGKGSVTILDPTKLQTDAYEAKRHRPIMVSGVVLHSLPSGYRFDLRRRQVRATLRP
                     VDATDRQLAHIEAASDRTRKLIRRIAAEGADDSAPERSRRRATRSRALEEASE"
     gene            1083..3872
                     /gene="cphA"
                     /locus_tag="VGO32_06800"
     CDS             1083..3872
                     /gene="cphA"
                     /locus_tag="VGO32_06800"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV7200494.1"
                     /translation="MTKRARTEGTGPRGTLRIVEQRVYRGPNYWSYEPTIKLIVDLGV
                     LEEYPTNKLEGFTDRLLGIIPGVASHSCGTGRPGGFEGRLREGTWLGHVAEHIALQIQ
                     RDAGTEVGRGKTRSTGQKGRYHVIFSYGEENVGLAAGRLAVKLVNHLVEADPTFDFRA
                     EFEQLVRLAERAAFGPSTQAILDEAALRDIPWIRLNEASLVQLGHGIHQKRIRATMTS
                     QTSSLGVDIAQDKKLTNRLLAATGVPVPRSEVVRSADEASTAAARIGYPVAIKPLDGN
                     HGRGVILNLADETAVRTAYPLARAESRFGGVVVESFLTGNDYRCLVVGGVLHAVAQRL
                     PAHVDGDGRKSVTDLISVTNADPRRGIGHEKVLTRIVVDAEAAAYAAEQGFALDAVPP
                     KGTRVFLKRTGNMSTGGISIDRTEEIHPENAEIAEQAAQVIGLDIAGIDFICPDISIP
                     VRETGGGIVEVNAAPGFRMHTNPTEGEPQYVARPVIDMLFPPGTASRIPIVAVTGTNG
                     KTTTARMISHILKGMGRKVGMTSTDGIFIDGRPIRRGDMSGPKSASMVLQNPLVDTAV
                     FEVARGGILREGLGYQRNDVAVVINVASDHLGLGGINSVRQLAGVKQVIVEAVPRSGT
                     AVLNADDPLVSKMAAACSGSVIYFSLNGDNETLRRQASRGRRSVTLESGRNGDLIVLR
                     QGRKTLPLVWTHLIPATFEGRAKMNVVNALAATAAAWAAGAHLHDIRQGLRTFATSYF
                     QAPGRLNLFDLDGYRVLVDYAHNPHAMTALGDFIDAISAEPTNGHSPLVTGRRIGVIA
                     TAGDRRDRDIRELGRVAASYFDRIVIREDQNPRGRERGATAALIREGVEAAVRDGARC
                     QKIEVVLDELDATRRALDQGRRGDLVVLCVDHANLAWKELQHRLHGTETAGPPVLPAT
                     NGAGSSADIDAEDFF"
     gene            complement(3894..5513)
                     /locus_tag="VGO32_06805"
     CDS             complement(3894..5513)
                     /locus_tag="VGO32_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV7200495.1"
                     /translation="MAADRPSEPAVELLELRVLEGPNRFANRPAIKIEFQGDPAAVVR
                     VARDAGEIVRHFHHELGFPAPRVTFRRSEDGTRAMAAFSWRRRAIAQAIAGAAARVAL
                     GQGTERRELRNLRAVAEGPRSVLPVPRVPIVAITGTNGKSTTTRLIAHITSQAGLRVG
                     MTNSDGIYVRGELVEAGDWTGFGGAGRILAEPDLDLAVLETARGGILLRGIGYEHNDV
                     SVVTNVSADHLGLHGIDTLDELAEVKGAVVRITRRDGWAVLNGQDPRVWAMRLHSRAH
                     PYAFSLSPRVPAVVEVLAQGGRAAVIERGAIVLRRPRGRRERIAAVDDLPVTFAGLSR
                     HNVANALAAAAACDALGLTSEQIRDGLMAFAQDERLNPGRLNLYSRRGVLALVDFAHN
                     EAGLAGLLDVCDRLVARPGGRVRLALGTAGDRSDEILGGMGQLAGRRADDLVISEKRH
                     YLRGRDLPEMNALFRRAAADGGYEGRVIAYRTELGALRALLARSHRGDVAAVMSHVER
                     ADIFAWLAREGYRAITLPRLRALLASRDHER"
     gene            5538..6713
                     /locus_tag="VGO32_06810"
     CDS             5538..6713
                     /locus_tag="VGO32_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV7200496.1"
                     /translation="MASPIPLTTLRDHVADVLPAYLADLEQLVGIDCGSYTPGGVNEV
                     ADVVSARLETLGASIERQEGGTAADGRPLGDLVVARLEGTGPRLLLIGHMDTVFEPGT
                     AAARPYRIDGERALGPGVSDMKGGLLAGIGALAALQVAGHRPAVTFVANPDEEIGSPF
                     SGPVIRQLAGDHQVALVLECARANGDIVSARKGIAELALTVRGRAAHAGVEPEKGRSA
                     ILAAARLTEALHGLNGRWPTVSCNVGTIAGGTRPNVVPDTCRLEVDLRAANGAEYGEA
                     LAEVARLAAEPTMPDTHTEHRLIAAHAPMEKSPATGRLVELAQAIAGELGFTVNDAAT
                     GGASDANTTAAEGVPTLDGLGPIGGDDHAPLEWLDLSSVIPRTTLLAGLMSRIDEAL"
     gene            6751..7191
                     /locus_tag="VGO32_06815"
     CDS             6751..7191
                     /locus_tag="VGO32_06815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7200497.1"
                     /translation="MTDSFRHHTRVEVRFRDIDAFGHVNNSAFVTYLEQARVRFLIDT
                     IQVDVIQSLPVILAAIHVDFRAPILFGQEIEIGTRVDWIGNTSFSMSHEMTARTDQGT
                     TRVVAEASTILVAYDYGTEQPIPVPDAWRAAFTAHEGRSLERPV"
     gene            7188..8606
                     /gene="lpdA"
                     /locus_tag="VGO32_06820"
     CDS             7188..8606
                     /gene="lpdA"
                     /locus_tag="VGO32_06820"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV7200498.1"
                     /translation="MTDEHHYDLLILGGGMSYVGAIRAAQLGMKVAVVERDRLGGTCL
                     NRGCIPSKALLETADLLHRVKALGPEFGLTHAEGIGLDPVNLGKRRDAVVEKHVKGVE
                     FLMKKNGVAVLRGHGVLTSPTSVRISGGESGDLEATATDLILATGSAPRHLPDLTVDQ
                     DRVIDSDEALRRDVLPKSVTIIGAGAIGVEWASLYGDLGADVRLVEFMDRIVPLEDPD
                     ISKELSRLFRKRGMTILPASTVDPKSLERTKTGIRHRVVANDGSGTTDVESDWILVAI
                     GRRPVTENIGLEQIPGVTVDRGFVVVDGHMRTGVPHMYAIGDVVPGYALAHVASHESI
                     VAVETIAGLDVDPVRMDLMPRVTFCRPEIASVGLSEAEATAAGTTVKVGSFPFRALGK
                     ATIVGDVDGFAKFVADATTGRLLGAHIIGPHAGDLLAEPTFARLVEGTAEEIAMSVHA
                     HPTLTEVLAEAALAVDGAALHV"
BASE COUNT         1353 a         3105 c         3042 g         1223 t
ORIGIN      
        1 agaaatcgag atcctggtgg atgacgtccg gcgacgaggg catccgacga gtgtacgggt
       61 ccgtgggatg ggtcgggatt gttagactcc tcggcaatgg ctgcgaccac ccgccctgcg
      121 cggccacgcg ccgggcacct cgtgatcatc ggcggcgcgg aggacaagct gcgccagcgg
      181 acgatcctga cccgcttcgt cacgctcgcc ggcgggccgg cggcgcggat cctggtcatc
      241 tccaccgcct cgtcgctggg ggacgaggcg accgacctgt acgcctccct gttccggcag
      301 ctgggcgtgg ccgaagtgac gagcgcgcgt ccgatgaccc gcgatgaggc taacgcgccc
      361 cgcatcgcgg acgagctgcg ggacgtgacc ggcgtgttcc tgaccggcgg caaccagctc
      421 cggctgtcga ccgtgatcgg cgggacaaag cttgggcgcg cgctggtgga cgcccacaag
      481 cgcggcctgg tcgtggccgg cacgagtgcc ggcgcctcgg cgatcgccag ccacatggtc
      541 gccttcgggg ccagcggcgc gacaccgaag cagcggatga cccagatgag cgccgggctc
      601 ggcctgctct ccggggtcgt catcgaccag catttcgagc agcgcaaccg tatcggccgc
      661 ttgatcgccc tcgtggccca atcaccatcc ctgctcggga tcggtctcga cgaggacacg
      721 gccgcggtcg tgtcaccgag cgggatgctg gaggtcatcg gcaagggctc ggtcaccatt
      781 ctggacccga ccaagctcca gaccgacgcc tacgaggcca agcgccatcg gccgatcatg
      841 gtcagcgggg tcgtcctcca cagcctgccg tccggctacc ggttcgacct tcgccggcgc
      901 caggtgcgcg cgacgctccg accggtggac gccaccgatc gccagctcgc ccatatcgag
      961 gctgcctcgg atcggacccg caagctcatc cggcgcatcg cggccgaggg cgcggatgac
     1021 agcgcacccg aacgctcgcg tcggcgggcc acacgctccc gcgcgctcga ggaagccagc
     1081 gagtgacgaa gcgcgcgcgg acggagggca ccggcccgcg cgggaccctc cggatcgtcg
     1141 agcagcgtgt ctaccgaggg ccgaactact ggtcctatga gcccacgatc aagctgatcg
     1201 tcgatctcgg cgtgctcgag gagtacccga ccaacaagct cgagggcttc accgatcggt
     1261 tgctcgggat catccccggc gtcgcctccc attcgtgtgg caccgggcga ccgggcggct
     1321 tcgaggggcg gctgcgcgaa ggaacctggc tcggccacgt ggccgagcac atcgcgctcc
     1381 agatccagcg tgacgccgga accgaggtcg ggcggggcaa gacccggtcg acaggccaga
     1441 agggccgcta ccacgtcatc ttctcgtacg gcgaggagaa cgtgggactc gccgccggac
     1501 gactggcggt caagctcgtc aaccacctgg tcgaggccga cccgaccttc gacttccgag
     1561 cggagttcga gcagctggtc cggctggccg agcgggccgc attcgggccc tcgactcagg
     1621 ccatcctgga cgaggccgcg ctccgtgaca tcccctggat ccggctcaac gaggcgtcgc
     1681 tcgtccagct gggccacggc atccaccaga agcggattcg ggccacgatg acgtcgcaga
     1741 cgagtagcct gggcgtggac atcgcccagg acaagaagct cacgaaccgg ctgctggcgg
     1801 caaccggcgt gccggtgccc cggtcggagg ttgtccgatc ggccgatgag gcctccaccg
     1861 cggcggcacg gatcggctac ccggtcgcga tcaagccgct cgacggcaac catggccgcg
     1921 gggtcatcct taacctggcc gatgaaacgg ccgtccggac cgcctacccg ctggcccgcg
     1981 ccgagtcccg cttcggtggg gtcgtggtcg agtcgttcct gaccggcaac gactaccgct
     2041 gcctggtcgt gggcggcgtc ctccatgccg tcgcccagcg actcccggcc catgtcgacg
     2101 gcgacggccg caagtcggtc accgacctca tctccgtcac caacgccgac ccgcggcgcg
     2161 ggatcgggca cgagaaggtc ctgacccgga tcgtggttga cgcggaggcg gcggcgtacg
     2221 ccgcggagca ggggtttgcg ctggacgcgg tgcctcccaa gggcacacgg gtcttcctca
     2281 agcggaccgg gaacatgagc accggcggga tcagcatcga tcggaccgag gagatccacc
     2341 ctgagaacgc ggagattgcc gagcaggcgg cccaggtcat cggcctggac atcgcgggca
     2401 tcgacttcat ctgcccggac atcagcatcc cggtccgcga gaccggtggc gggatcgtgg
     2461 aggtcaacgc cgcacccggc ttccggatgc acaccaaccc gaccgagggc gagccgcaat
     2521 acgtcgcccg gccggtgatc gacatgctct tcccacccgg gaccgcctcg cggatcccga
     2581 tcgtggccgt caccggaacg aacggcaaga caaccaccgc ccggatgatc agccacatcc
     2641 tgaagggcat gggccgcaag gtcgggatga ccagcaccga cgggatcttc atcgacgggc
     2701 gaccgatccg gcgcggggac atgagcggcc cgaagtcggc gtcgatggtg ctccagaacc
     2761 cgctggtcga taccgcggtg ttcgaggtgg cgcggggcgg gatcctgcgc gaggggctcg
     2821 gctatcagcg caacgacgtg gcggtcgtga tcaacgtcgc gtcggaccat ctcggcctgg
     2881 gtggcatcaa cagcgtgcgc cagctggcgg gggtcaagca ggtgatcgtg gaggccgtgc
     2941 cccgcagcgg gaccgcggtc ctgaacgccg acgacccgct ggtgagcaag atggcggccg
     3001 cctgctccgg gtcggtcatc tacttcagcc tgaatgggga taacgagacg ctccgccgcc
     3061 aggcctcacg gggccggcgg tcggtgactc tcgagtcggg ccgcaacggc gacctgatcg
     3121 tcctccgcca ggggcgcaag acgctgcccc tggtgtggac ccatctcatc ccggccacgt
     3181 tcgagggtcg ggccaagatg aacgtggtca acgcgctggc cgcgaccgcg gccgcgtggg
     3241 ccgcgggcgc gcatctccac gacatccgcc agggattgcg gaccttcgcg acctcgtact
     3301 tccaggcgcc cggccggctc aacctgttcg acctggacgg ctatcgggtc ctggtcgact
     3361 atgcccacaa cccccatgcc atgaccgccc ttggcgattt catcgatgcg atctcggcgg
     3421 agccgaccaa cggccactct cctctggtca ccgggcgccg gatcggggtc attgccacgg
     3481 ccggcgatcg gcgcgaccgc gacatccgcg agctgggtcg ggtggcggcg agctacttcg
     3541 accggatcgt gatcagggag gaccagaacc cccgcggccg tgagcggggg gcgaccgccg
     3601 ccctcatccg ggaaggggtg gaggccgccg ttcgcgacgg cgcccgctgc cagaagatcg
     3661 aggtcgtcct cgatgagctg gatgccaccc ggcgcgccct cgaccagggc cggcgaggcg
     3721 atctggtcgt cctgtgcgtg gaccacgcga acctggcctg gaaggagctc cagcatcggc
     3781 tccatggcac cgagaccgcc ggaccgccgg tcctgccggc caccaacggg gccggctcgt
     3841 cagccgacat tgacgccgag gacttcttct gaccgatgcg cggccggcgc ggcctagcgc
     3901 tcgtggtcgc gtgaagccag gagggccctc agcctgggca gggtgatcgc ccggtagccc
     3961 tcgcgggcca gccaggcgaa gatgtcagcc cgctcgacgt ggctcatcac cgcggcgaca
     4021 tcgccccgat ggctccgggc cagcagggcg cgaagcgcgc cgagctcggt ccggtacgcg
     4081 atcacccgac cctcgtatcc gccgtcggcc gccgcccgtc ggaacagggc gttcatctcc
     4141 ggcagatctc gcccgcggag gtagtggcgc ttctcgctga tgacgaggtc gtccgcccgc
     4201 cgtcccgcga gctgccccat cccgccgagg atctcgtcgc tccggtcacc ggccgtgccc
     4261 agcgccagcc ggacgcggcc gcccggtcgt gccacgagcc ggtcgcagac gtcgagcagg
     4321 ccggcgagcc cggcctcgtt gtgggcgaag tcgaccagtg ccaggacacc acggcgggag
     4381 tagaggttga ggcggccggg gttcagacgc tcatcctggg cgaacgccat gagcccgtcg
     4441 cgaatctgtt cgctcgtaag ccccagcgcg tcgcaggcgg cggcggcggc cagggcattg
     4501 gccacgttgt ggcgggacag cccggcgaag gtgaccggca ggtcgtccac cgccgcgatc
     4561 cgctcccgcc gaccccgcgg ccggcggagg acgatcgcgc cccgctcgat gaccgccgcc
     4621 cgccctccct gggccaggac ctcgaccacg gcaggcactc gaggggagag gctgaaggcg
     4681 tacggatggg cacggctgtg gaggcgcatc gcccacaccc gcgggtcctg tccgttcagg
     4741 acggcccagc cgtcccggcg cgtgatgcgg acgacggcac ccttcacctc ggccagctca
     4801 tccagggtgt cgattccgtg caggcccagg tggtcggcgc tgacgttggt gacgaccgag
     4861 acgtcgttgt gctcgtatcc gatgccgcgg aggaggatgc cgccgcgcgc cgtctcgagc
     4921 acggccaggt caagatccgg ttcggccagg atccgtcccg ccccgccgaa tcccgtccag
     4981 tcccccgcct cgaccagctc gccgcggacg tagatcccgt ctgaattcgt catcccgacc
     5041 cgcaacccgg cctgggatgt gatgtgggcg atcagccggg tcgtcgtgct cttcccgttc
     5101 gtgccggtga tggccacgat cggcacccgg ggtacgggaa gcaccgaccg cgggccctca
     5161 gcaaccgccc gcaggtttcg cagctcgcgg cgctcggtcc cctggccgag tgccacccgc
     5221 gcggccgcgc cggcaatcgc ctgcgcgatc gcacgccgtc gccaggagaa tgccgccatc
     5281 gcccgggtcc cgtcctcgga tcggcggaat gtgacccgcg gcgcgggaaa gccgagctcg
     5341 tggtggaagt gacggacgat ctcgcccgcg tcccgcgcca cccggaccac cgccgccgga
     5401 tcgccctgga actcgatctt gatcgccggc cggtttgcaa accggttcgg gccttccagg
     5461 acgcgcaatt cgagcagctc gacggccggc tcggacggac ggtcggcggc catggcggcc
     5521 agtatagttt cggccccatg gcgtccccga tccccctgac cacgctccgc gaccacgttg
     5581 ccgacgtcct gcccgcctac ctcgccgacc tcgagcagct ggtcggcatc gactgcggct
     5641 cgtacacgcc aggcggggtc aacgaggtgg cggatgtggt cagcgcccgg ctcgagacgc
     5701 tgggcgcctc gatcgagcga caggagggcg ggacggcggc cgatgggcgt ccactcggcg
     5761 accttgtggt cgcccgcctg gagggcacgg gcccccggct gctcctgatc ggccatatgg
     5821 acaccgtctt cgagccgggc accgcggccg cccgcccgta tcggattgac ggcgagcgcg
     5881 ccctcgggcc cggcgtctcc gacatgaagg gtggcttgct ggccggcatc ggcgccctgg
     5941 cggctctcca ggttgccggc catcggcccg ccgtcacatt cgtggccaac ccggacgagg
     6001 agatcggctc gcccttctcg ggaccggtca tccggcagct ggccggtgac caccaggtgg
     6061 cgctcgtcct cgaatgtgcg cgggcaaacg gggacatcgt gagcgcccga aagggcatcg
     6121 ccgagctggc gctcaccgtg cgcggacggg cggcccatgc cggcgtcgag cccgagaaag
     6181 ggcggagcgc gatcctggcc gccgcccgtc tgaccgaggc gctccacggt ttgaacggcc
     6241 gctggccgac ggtgtcctgc aacgtgggca cgatcgcggg cgggacccgg ccgaacgtcg
     6301 ttccggacac gtgccggctg gaggtggacc tccgcgcggc caacggagcg gaatatggcg
     6361 aggccctggc cgaggtagcc cggctggcgg ccgagccaac gatgcccgac actcataccg
     6421 agcaccggct catcgccgcg cacgccccga tggagaagtc tccggccacc ggtcggctgg
     6481 tcgagctggc ccaggccatc gccggcgagc tgggcttcac cgtcaacgac gcggccaccg
     6541 ggggagcgtc ggacgcgaac acgaccgccg ccgaaggcgt cccgaccctc gacggactcg
     6601 gcccgatcgg cggtgacgac cacgccccgc tcgaatggct ggacctgtcc agcgtcattc
     6661 cgcggacgac gctgctggcc gggctcatga gccggatcga cgaggccctc tgaccgatga
     6721 agcggcagtg accgctagaa tcgggcctcc gtgaccgatt ccttccgcca tcacacacgc
     6781 gtcgaggtcc ggtttcggga tatcgatgcg ttcggccatg tcaacaactc ggcgttcgtg
     6841 acctacctcg agcaggcgcg cgtgcgcttc ctgatcgaca ccatccaggt cgacgtcatc
     6901 cagagcctgc cggtcatcct ggccgccatt cacgtggact tccgcgcgcc catcctgttc
     6961 ggccaggaga tcgagatcgg gacccgggtg gactggatcg ggaacacgag cttctcgatg
     7021 tcccacgaga tgacggcccg gaccgatcag ggcacgacgc gggtggtggc cgaggcgagc
     7081 acgatcctgg tggcgtacga ctacggcacg gagcaaccga tcccagttcc cgatgcctgg
     7141 cgcgccgcct tcaccgcaca cgaggggcgc agcctggagc gacccgtatg accgacgagc
     7201 accactacga cctgctgatc ctcggcggcg gcatgagcta cgtgggcgcc atccgcgccg
     7261 cccagctcgg gatgaaggta gcggtcgtgg agcgcgaccg gctgggcggc acctgcctga
     7321 atcgtggctg catcccgtcc aaggcgctgc tcgagaccgc ggacctgctg caccgggtga
     7381 aggcgctcgg tcccgagttc gggctcaccc acgcggaggg catcgggctc gacccggtga
     7441 acctcggcaa gcgtcgggac gcggtcgtcg agaagcacgt caagggcgtc gagttcctga
     7501 tgaagaagaa cggggtggcc gtcctccgcg gccatggggt gctcaccagc ccgaccagcg
     7561 ttcggatcag cggcggggag tcaggcgacc tggaggccac cgccaccgat ctcatcctgg
     7621 cgaccggcag cgcgcctcgc catctgcccg acctgaccgt cgaccaggat cgggtcatcg
     7681 actcggacga ggccctccgc cgcgacgtcc tgccgaagtc ggtcacgatc atcggcgcgg
     7741 gcgccatcgg cgtggagtgg gcaagcctgt acggcgacct gggagctgat gttcggctcg
     7801 tcgagttcat ggaccggatc gtcccgctcg aggacccgga catcagcaag gagctgagcc
     7861 gcctcttccg caagcgcggc atgacgatcc tgcccgcctc gaccgtggat ccgaaatcgc
     7921 tcgagcggac caagaccggg atcaggcaca gggtcgtggc caacgacggc agcgggacga
     7981 ccgacgtcga gagcgactgg atcctggtcg ccatcggccg gcggccggtg accgagaaca
     8041 tcggcctcga gcagatcccg ggcgtcaccg tcgaccgcgg gttcgtggtg gtggacggcc
     8101 acatgcgcac cggcgttccg cacatgtacg ccatcggtga cgtcgtcccc ggctatgccc
     8161 tggcccacgt cgccagccac gagtcgatcg tggcggtcga gaccatcgcc ggcctggacg
     8221 tggacccggt ccggatggac ctcatgccgc gggtgacgtt ctgccggccc gagatcgcga
     8281 gcgtgggtct gagcgaggcc gaggcgacgg cggccggcac gacggtcaag gtcggcagct
     8341 tcccgttccg tgccctcggc aaggcgacga tcgtgggcga cgtcgacggc ttcgcgaaat
     8401 tcgtggccga tgccactacc ggtcggctgc tcggggcgca tatcatcggg ccccacgccg
     8461 gcgacctgct ggcggagccg acctttgccc gcctggtgga ggggacggca gaggagatcg
     8521 cgatgtcggt gcatgcccat ccaaccctga ccgaggtcct ggccgaggcc gccctggcgg
     8581 tcgacggcgc ggccctccac gtctgaccga tgaccgacgc tgacgccgag accaaggtcc
     8641 gccgcgtgct cggcctggac acgaccaacg gccaggccga cggctcggca gcgacgcctg
     8701 caccgaaggc cgcgccatcc acc
//
LOCUS       DASYGU010000242         9019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1324145, whole genome shotgun sequence.
ACCESSION   DASYGU010000242 DASYGU010000000
VERSION     DASYGU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324145"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGO32_06825"
     CDS             <1..580
                     /locus_tag="VGO32_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200499.1"
                     /translation="SQVAALDARVEAAVQTAAPRPRRNRAVRTVLLLAALFVIVPSIL
                     GVGAAIMSTEDPFGLTDAAGFQAELDSAMAITPIPAGATWPDFLAADSAAAYSAGGGR
                     AWVELVSMCLWEVEWLHARAAVDATREALARITIIGFPEWYSTDSVFFQPQGSRALYL
                     TPIVAGVRVGDPTALARDVDLNCAGIAPFVAP"
     gene            complement(622..1374)
                     /locus_tag="VGO32_06830"
     CDS             complement(622..1374)
                     /locus_tag="VGO32_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200500.1"
                     /translation="MKKLEIGCALALVALLGACATGSPSAAEPSATSSAETAASVEPT
                     NGSAYPRMAGPLEAGTHVLIAESSALDPILVRVQLPDHWAGSDGFVLITGGGISSRQG
                     AAIQFWGAPDYTYTDACHWSTTQFPVEKTVDFMASALAAQQPRHASTPREISVGDRRA
                     IEIQLSVPDDVVPSTCDLYEGDGFFQSWHYGSGESARYHQGPGQQDLIRLVDVDGRLV
                     IIDAATWPELPDGTRAELMAVLDSLSIDLPPE"
     gene            complement(1490..2197)
                     /locus_tag="VGO32_06835"
     CDS             complement(1490..2197)
                     /locus_tag="VGO32_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200501.1"
                     /translation="MRVPELWAEATLRAVAEPLSAPQTRSNYMLPVGLATAAIVIVLI
                     VGLGLLWGPTIGTPDATPTPEATPGSTSSSPDPSASIPLIQRMPGSRSNPAGVYGWTG
                     ALGSSTGMHNVVGLDDGGGRMTQMVFAVEDDCFASGDGPEPVPVTVGGLDGTYVEPYD
                     GPGVLFMPEREEGQTTGAYALAIDGRTLCVYLTWDPSTTADELDAARQVVESIRGEAL
                     GSGLRIIFTLPQGWDTG"
     gene            complement(2233..2700)
                     /locus_tag="VGO32_06840"
     CDS             complement(2233..2700)
                     /locus_tag="VGO32_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7200502.1"
                     /translation="MTAVADDLVQLEAVYREEAERLWRALLAFSRDPDVASDAVAEAF
                     ARAIRDRAAIRDPRAWVWRMGFRLAAADLTQRSRANSQMPNTGYLETEADADLLDALA
                     GLPEKQRTAVILFYYVDAPIREIAEKTGMSQLAVRANLSRGRKRLKHLLGESR"
     gene            2893..3465
                     /locus_tag="VGO32_06845"
     CDS             2893..3465
                     /locus_tag="VGO32_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200503.1"
                     /translation="MPTSTKEITVDHLTLKSRGRAVSLLAASLALAVAACTPSGSPAG
                     SNPADGGGGAPSGAGSSAPDGGQPGSGEDGVEGSLTTSGVYDATWTWEPGNSMEIGDI
                     GGITLNSDQGTFGNITVKTDGSISFGSTAPELPVLYYEGTGAEVTLDTVNIGYVCSFT
                     LDDDLTGSDGSVLHIAGSMTFHLDDEITPC"
     gene            3496..6297
                     /locus_tag="VGO32_06850"
     CDS             3496..6297
                     /locus_tag="VGO32_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7200504.1"
                     /translation="MEAQPSAGAVVVGRDAERRAIDAWLEAGRPASLLINGEAGIGKS
                     TLWAYAADRAADRGDRVLAWRASNAEHELAFAALTALFDEPMVVPLLDRLPDPRRRAL
                     ETAIGRVSPDLDRPEPSLVGLAVADVLRILSAEGPVVVAIDDVQWTDAASAEALAFAA
                     RRLRTEPVGFVLALRTGPDVSGEADLLSSAVERSERLHVGPLSVGALGRLVHERLDVA
                     HPRPLLVRLHEACSGNPFLGLGISRSLQARGMEPALGEPFPVPPQAEPVVRDHLATLS
                     RDARRSVVIVAMSPSPSVALVERVLREGGGRAVDEACEKGVLVADGSRLRAVHPLFTS
                     TAYADAPPGERRALRAALADIAEDPVERAIHQAAMVAGPDADVAEALAAAGRTAFARG
                     APAIAADLLERSAHHAVGVEQRSIALIAAADAAVAAGDYDRAATSLRAVLDAVPHGRR
                     RAEALLALGEVVYLEDPNEALVLLVSALDHTDGDPLLEALVHSHIAGMADMDPGTAQR
                     SALAAVDILERPGLKPVQDHLACALLDRAFHWLLACEKVAVDDIDRAIALRSGSGVSF
                     VSRRAQEVAHRCSYHLGRLDDAIALDQAEYRRLTDLGQVGLLPPIIQSLTALLLMTGD
                     WEAARQYARECHDLVEQGEEAWRERSLTIDAHVAALSGDLETSRAIGMEALTRQEATG
                     DLWEGTIFCAILGFVELSASDHVAALRYLTRALEQADAIEVALPSQFGFLGDLVEAAV
                     LSGEIDLAEQVLHDRLEEPATRVPLPWILAMAARGRGLVAAGRGELDDALGHLDRAEE
                     LFRATLGMPFERARTLLARGQVHRRAGQRRKAREDIGAALETFQALGAAAWAARARQE
                     LGRIGGRTTSGSALTESERAVAELAAAGRSNREIAAELVVSVRTVESQLSAAYRKLDI
                     QSRGQLTAALAVSGGPTAG"
     gene            6368..6670
                     /locus_tag="VGO32_06855"
     CDS             6368..6670
                     /locus_tag="VGO32_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEV7200505.1"
                     /translation="MTANAKRRFFLVERYIPSISSSSVESAARRLAETTDDSAHHLMT
                     LLVAGEETCLSVFEASDAHAVERANERAGFELDRIVEVALFPEAPVGEGPLAGEGC"
     gene            complement(6689..7318)
                     /locus_tag="VGO32_06860"
     CDS             complement(6689..7318)
                     /locus_tag="VGO32_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEV7200506.1"
                     /translation="MRSRVGLVLVVLGLAMVVIASPAAAGAPLPSADLAITKSDTPDP
                     VVAGGTVSYAVVVENLGPDAATGVSWTDTYPVQLSPGLMGWPAGWTQSTLGNTVTFTA
                     SAPLAADGPRNFLLEFTVSPSVPDGTILTNDATVTASTADPTPGNNSASAQTTVVMPP
                     PTPAASLADAAMAPSEPSGPLTSLGFAVLLFGALGALAVMSGRRVLPRR"
     gene            complement(7404..8255)
                     /locus_tag="VGO32_06865"
     CDS             complement(7404..8255)
                     /locus_tag="VGO32_06865"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEV7200507.1"
                     /translation="MADHPRPFHAPIRFTAPDGTPLHSPMVEAEIGGVSTRLVLDTGS
                     DTHLLTAEFAEAAGLAVEPGEAGMDHAGTEIESGTAGTVPIRIGEDTLALHSVVVIPA
                     PPPFPGWGIGGIVSPQFLHPTAWIVIDMADDALVFAETDEAELMSRLTSRHPDWPILV
                     LDRIEDGTTVVQAAIEPHPPIPTLINTGGKSTEFARAAIPDRLGGQSVRLGGGVSGAD
                     VMGTRLGPAVLAIGGMRIPVADLTSRDEMDGVQGMVGMDVLRGTILACTAHPAGRVIW
                     QVALVPF"
     gene            8279..8704
                     /locus_tag="VGO32_06870"
     CDS             8279..8704
                     /locus_tag="VGO32_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200508.1"
                     /translation="MRATPAVRFRLMAAAVVIGLVVAGCALPATEEGFNQGFCEVDLA
                     FETAAAAIVEIEDREEWIDATRDAMLDFRDALTRLPVWEPARDAKGELANALNAIVRL
                     IESGVAGELVESAQWENALETMLTARENLRGVAGPCISQ"
     gene            complement(8705..>9019)
                     /locus_tag="VGO32_06875"
     CDS             complement(8705..>9019)
                     /locus_tag="VGO32_06875"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200509.1"
                     /translation="DRTDRLLPVIGPGGGDVVAVHQDASVFVSSLAPGGEVSHQIGPG
                     RGVYCYLIEGAANFNGEDLSTGDAVKVTDESELTIHATGSSELILVDVPMTFEPVGIW
                     RR"
BASE COUNT         1373 a         3035 c         3180 g         1431 t
ORIGIN      
        1 gtcccaggtc gctgcgctgg atgcccgggt tgaggctgcg gtgcagaccg cggcaccgcg
       61 accgcgccgc aaccgggcgg ttcgcaccgt cctcttactg gccgccctgt tcgtgatcgt
      121 gccctcgatc ctgggcgtgg gcgcggcgat catgagcacc gaggacccct ttggcctgac
      181 cgatgccgcc ggcttccagg ccgagctgga cagcgccatg gcgatcacgc ccatcccggc
      241 cggcgcaacc tggcccgact tcctggccgc cgattccgcg gcggcctaca gcgccggcgg
      301 tgggagggct tgggtcgagc tagtctcgat gtgcctatgg gaagtggagt ggctccatgc
      361 ccgcgctgcc gtcgatgcga cccgcgaggc cctggcccgg atcacgatca tcggcttccc
      421 cgagtggtat tcgaccgaca gcgtgttctt ccagccgcag ggttcgcggg ccttgtacct
      481 gaccccgatc gtggcagggg ttcgggttgg cgaccccacc gccctggccc gggacgtcga
      541 cctcaactgt gcaggaatag cgcccttcgt cgccccctga cgcctttggg ttcagacgcc
      601 ataggtgcgc cgctcggctc gctactccgg ggggagatcg atcgatagcg aatcgaggac
      661 ggccatcagt tctgcgcggg tcccatccgg cagctcaggc catgttgccg cgtcgatgat
      721 caccagccga ccgtccacgt cgacgagccg gatcagatcc tgttggccgg ggccctggtg
      781 gtaacgggcc gactcgccgg aaccgtagtg ccagctctgg aagaatccgt ccccttcgta
      841 caggtcgcac gttgagggga cgacgtcgtc gggcaccgaa agctgaatct cgatcgctcg
      901 gcggtcgcct accgagatct cgcgtggagt cgatgcgtgt ctcggctgct gagcggccag
      961 ggctgacgcc atgaaatcga ccgtcttctc caccgggaac tgggtggtcg accagtggca
     1021 cgcgtcggtg taggtgtagt ccggcgcacc ccagaactga atggctgccc cctgccgact
     1081 gctgattccg ccgcccgtga tcagcacgaa gccatcgctt ccggcccaat gatcgggaag
     1141 ttggaccctc accagaatcg ggtcgagcgc cgacgactcg gcaatgagga catgggtacc
     1201 ggcctcgagt gggccggcca tccgtgggta tgccgagccg ttggtcggct caacggacgc
     1261 cgccgtctcc gcgctggacg tcgcagatgg ctcggccgcg gaaggcgacc ccgtcgcgca
     1321 cgcccccaac agagcgacga gcgcaagcgc gcacccaatt tcgagctttt tcatctggcc
     1381 tctccctccg cttcgaaaat gccgtactga ccctgggcct cccgcgacga ttcgggccaa
     1441 caggccgcct caacccccac ctcggctatc gcgggtcccg gcgagcgaat caaccggtgt
     1501 cccagccctg cggaagcgtg aagatgatcc gaaggccgct gccaagagct tcgccgcgga
     1561 tcgactccac gacctggcgg gctgcgtcca gctcgtccgc tgtggttgac gggtcccagg
     1621 tgaggtagac gcacagggtg cggccatcga tggccagggc atacgctccc gtggtctgtc
     1681 cctcttcccg ttccggcatg aagaggacac ccgggccgtc gtagggctcg acatacgtgc
     1741 catcgaggcc accgaccgtc accggaaccg gctcggggcc atccccgctg gcgaagcagt
     1801 cgtcctcgac ggcaaagacc atctgcgtca ttcgaccgcc gccatcgtcc agcccgacga
     1861 cgttgtgcat ccccgtgctc gatcccagtg cgccggtcca tccgtatacg cccgccgggt
     1921 tggatctcga cccgggcatc cgctggatga gggggatcga ggccgaggga tcaggggaac
     1981 tggatgtcga gccaggggtg gcttcggggg ttggcgtcgc gtcgggcgtt ccgatcgtcg
     2041 gcccccacag caggccgagt ccgacgatga gcacgatgac aatcgccgcc gtcgccaggc
     2101 ccacgggcag catgtagttc gacctggtct gtggcgcaga caggggctcg gcgacggcgc
     2161 gaagcgtggc ctcggcccac agttctggca ctcgcacccg atcaaggctg cggaaacggt
     2221 cgtgcacgtc agtcatcgcg attcacccag gagatgcttg agccgcttcc gcccgcggct
     2281 gaggttggct cgcacggcaa gctgcgacat gccggtcttt tcggcgatct cgcggatcgg
     2341 tgcgtcgacg tagtagaaga ggatgacggc ggtccgctgc ttttccggca gaccggccag
     2401 ggcatcgaga aggtccgcgt ctgcctcggt ctcgaggtag ccggtattcg gcatctgtga
     2461 gttcgcccgc gagcgttgcg tgagatcggc ggcggcgaga cggaagccca tgcgccacac
     2521 ccatgcccgc ggatcgcgga tggcagctcg atcgcggatg gcccgggcga aggcttcagc
     2581 gacggcgtcg ctggcgacgt ccgggtcgcg gctgaacgcg aggagggcgc gccacaatcg
     2641 ctcggcttcc tcgcggtaga ccgcctcgag ctgaacgagg tcgtcggcga ctgccgtcac
     2701 tgataccact atgaactgat agagccgaaa tccggccaaa aggtgacatg agcgacgaga
     2761 tcaccccctg ctgctcggga gctgtttcct tggtcgcccc tgaggctggg tgcatcggcc
     2821 gatcccgttc ggcggaccaa agttgcgtgg tttcacggat gcgatggcga cagccacaga
     2881 cctacggtct ttgtgccaac gagcaccaag gagatcacgg tggaccatct caccctgaaa
     2941 tcgcgagggc gcgccgtgtc actgctggcg gcctccctcg cgctggccgt cgccgcatgc
     3001 acaccatcgg gcagtccggc cggaagcaac ccggccgatg gcggcggcgg agcgccaagc
     3061 ggggcaggat cgtcggctcc ggatggcggt cagcccggtt ccggtgagga cggcgtcgaa
     3121 ggcagtctca ccacgtccgg ggtgtatgac gcgacctgga cctgggaacc gggcaattcg
     3181 atggaaatcg gtgacattgg cgggatcacg ctgaattcgg accaggggac cttcggcaac
     3241 atcaccgtca agaccgatgg cagcatttcg ttcggaagca ccgcgcccga gctcccagtc
     3301 ctctactacg agggcaccgg cgcggaggtg acgctcgaca ccgtcaacat cgggtacgtg
     3361 tgcagtttca ccctggacga cgatctgacc ggcagcgatg gctccgtgct gcacatcgcc
     3421 ggctccatga cgttccatct ggacgacgag atcactccct gctgacatga gccaggcctc
     3481 cgctaccatc gcgaggtgga ggcacagcct tcggccgggg cggtggtggt cgggcgggat
     3541 gcggagcgcc gggcaatcga cgcgtggctc gaggcgggcc gacctgcgtc gctgctcatc
     3601 aacggcgagg ccggcatcgg caagagcacg ctgtgggcgt atgcggctga ccgagcggcg
     3661 gatcgagggg accgcgtgct ggcctggcgt gcgagcaacg ccgagcacga gctggccttc
     3721 gccgcgctga ccgcactctt cgacgaaccg atggtcgtcc ccttgctgga ccgcctgccc
     3781 gatccccggc gacgggcgct ggagaccgcc atcggtcgcg tcagcccgga cctcgatcgg
     3841 cctgagccga gcctggtggg gctggccgtg gccgacgtcc tgcgcatcct ctccgccgaa
     3901 ggccctgtgg tggtcgccat cgatgacgtg caatggaccg atgcggcgag cgcggaggcc
     3961 ctcgcctttg cggctcggcg cctgcggacc gagccggtcg ggttcgtcct tgccctccga
     4021 accggtcccg acgtgtccgg tgaggccgac ctcctctcct cggccgtcga acgatccgaa
     4081 cggctgcatg tcggaccgct gtcggtgggc gcgctgggcc gtctcgtcca cgagcggctg
     4141 gatgtggcgc atccccgacc gctgctcgtc cgcctccacg aggcctgctc gggcaacccc
     4201 ttcctgggcc tggggatcag tcgatcactc caggcgcgag ggatggagcc ggcgctgggt
     4261 gagcccttcc ccgtcccccc gcaggccgaa ccggtcgtcc gggatcacct cgcgacgttg
     4321 agccgggacg cccgacggag cgtggtcatc gtggccatgt cgccctcgcc gagcgtggcg
     4381 ctcgtcgagc gcgttctgcg cgagggcggc ggacgggcgg tcgacgaggc gtgtgagaag
     4441 ggtgtcctgg tcgccgacgg ctcccggctg cgggcggtcc accccctgtt cacctcgacc
     4501 gcctacgctg atgcgccgcc cggcgagcgg cgcgcgctcc gggcggcact cgcggacatc
     4561 gccgaggacc ccgtcgaacg agccattcat caggcggcca tggtcgccgg gcctgacgcg
     4621 gatgttgctg aggcactcgc cgccgcaggc agaaccgcct tcgcccgcgg cgcacccgcg
     4681 attgccgccg acctgcttga gcgctcggct caccacgcgg tgggggttga gcagcggagc
     4741 atcgcgctga tcgcggccgc ggatgcggcc gtggcggcgg gggactacga ccgagcggcg
     4801 accagtctcc gggcggtgct ggatgccgtc ccccacgggc gtcggcgggc ggaggcgctc
     4861 cttgcgctgg gtgaggtcgt ctacctcgag gacccgaacg aggccctcgt gctcctcgtg
     4921 tctgccctcg atcacaccga cggggatcct ctcctcgagg cgttggtcca ctcccacatt
     4981 gccggcatgg cggacatgga tcccgggacg gcgcaacgga gcgcgctggc cgcggtggac
     5041 atcctcgaac gacctggcct caagccggtg caggaccacc ttgcctgcgc actgctcgat
     5101 cgggcgtttc attggctgct cgcgtgcgag aaggtcgccg tggacgacat cgatcgcgcc
     5161 atcgcgctgc gcagcggctc cggcgtctcg ttcgtttccc ggcgggccca ggaggtcgcc
     5221 catcggtgct cgtatcacct cggccggctc gacgacgcga tcgcgctgga ccaggcggaa
     5281 taccgacggc tcaccgacct gggacaggtg ggcctcctcc cgccgatcat ccagtcgctg
     5341 accgcgcttc tgctcatgac cggcgactgg gaggccgccc ggcagtacgc ccgggagtgc
     5401 catgacctgg ttgagcaggg cgaagaggcc tggcgcgaac gatccctgac cattgacgcg
     5461 catgtcgccg cgttgtccgg ggacctggag acgtcccgcg cgataggcat ggaggccctg
     5521 acccggcagg aggctaccgg cgacctgtgg gagggcacga tcttctgcgc catcctgggg
     5581 ttcgtcgagc tctctgcttc cgaccacgtc gcggccctcc gatatctgac ccgcgcactg
     5641 gaacaagccg atgcgatcga ggtcgcgctc ccatcccagt tcgggttcct tggcgacctg
     5701 gtcgaggcgg cggtgctgtc gggagagatc gacctcgccg aacaggtgct gcacgaccgc
     5761 ctcgaggagc cggcgacgcg tgtgcccctg ccgtggatcc tcgcgatggc cgcgcgaggt
     5821 cgtggcctcg tggctgccgg gcggggagag ctcgacgacg cacttgggca ccttgatcgt
     5881 gccgaggagc tcttccgcgc cacattgggg atgcctttcg aacgggcacg aacgctgctc
     5941 gcgcgcggcc aggtccatcg gcgagcgggc cagcgccgga aggcccgcga ggacatcggt
     6001 gccgccctcg agacctttca ggcgctcggc gcggcggcgt gggcggcgcg ggcaagacag
     6061 gagctgggcc ggatcggggg acgaacgacc agtggctccg cgctcaccga gtccgaacga
     6121 gccgtggccg agctggccgc ggccgggcgg tcaaaccggg agatcgcggc ggagctcgtc
     6181 gtctcggtcc ggacggtgga aagccagctg tcggcggcat atcggaagct cgacatccag
     6241 tctcgcggcc agctcaccgc cgccctggcc gtctccgggg gcccgaccgc cggctaggcc
     6301 cgcttggccc caaagttccg tggtttcacg gatgcgaccg atggggcctg ccacctacgg
     6361 tcgatcggtg acggcaaacg caaaacggcg gtttttcctg gtcgagcgct acatcccgtc
     6421 gatcagctcg agctcggtgg aatcggctgc ccgccgcctg gccgaaacga ccgacgactc
     6481 ggcccaccac ctgatgacgc tgctggtggc cggcgaggag acctgcctgt ccgtgttcga
     6541 ggcatccgac gcgcacgccg tggaacgcgc caacgaacgc gcgggtttcg agctcgaccg
     6601 gatcgtggag gttgcgctgt ttccggaggc tcccgtcgga gaggggcctc tggctggcga
     6661 gggctgctga cctggcagag gcggaagcct atcggcgggg tagcacccgt cgaccgctca
     6721 tcacggccag ggcgccgagc gccccgaata gcaggaccgc gaagccgagc gacgtcagcg
     6781 gaccggacgg ctccgacggc gccatcgctg cgtcggcgag cgatgcggcg ggggtcggtg
     6841 gcggcatgac caccgtcgtc tgcgcgctcg cgctgttgtt ccctggtgtc ggatcggcgg
     6901 tcgatgcggt gacggtggcg tcgttggtca ggatcgtgcc gtcgggaacg ctcggactta
     6961 cggtgaactc gaggaggaag tttcgagggc catccgccgc gaggggggcc gacgcagtga
     7021 acgtcacggt gttcccgagg gtcgattgag tccacccagc cggccagccc atgagccctg
     7081 ggctgagctg caccggatac gtgtcggtcc acgaaacccc ggttgcggcg tcaggcccca
     7141 ggttctcgac gacgacggcg tacgagaccg tgcccccggc cacgaccggg tcgggggtgt
     7201 cggacttggt gatcgcgagg tccgctgagg gcaggggcgc gccggccgcc gcagggctgg
     7261 cgatgacgac catcgccagg ccgaggacga cgaggaccag gccgactcgg gagcgcatgg
     7321 tggtttcctc aagtacgggt gcgaagagtc agagatgaaa ctcagccgcg cacgcgatca
     7381 aactacaccc tcggacttgg atttcagaag gggacgaggg ccacctgcca tatgacgcgg
     7441 ccggctgggt gggcggtgca ggccaggatc gtgccgcgga ggacgtccat cccgaccatg
     7501 ccctggacgc cgtccatctc gtcgcgcgag gtgaggtccg cgaccgggat gcgcatcccg
     7561 ccgatggcca ggaccgcggg gcccaggcgc gtacccatga catcggcgcc gctcacccca
     7621 ccgcccagcc gtacggactg accgcccagc cgatcgggga tggcagcgcg ggcgaactcg
     7681 gtggacttgc cgccggtgtt gatcagggtc gggatcggcg ggtggggttc gatggcggcc
     7741 tgaacgaccg tggtgccgtc ctcgatcctg tcgaggacga ggatcggcca gtccgggtgg
     7801 cgactcgtga gccggctcat gagctctgct tcatcggtct cggcgaagac cagggcgtca
     7861 tcggccatgt cgatcacgat ccaggccgtg gggtggagga actgggggct gacgatccca
     7921 ccgatgcccc agccggggaa tggcggcgga gccgggatga cgaccaccga gtgcagggcc
     7981 agggtgtcct ccccgatccg gataggaacc gttccggccg tcccactctc gatctcggtg
     8041 cccgcatggt ccatgccggc ctcgccgggc tcgaccgcga gacctgccgc ttccgcaaac
     8101 tccgcggtca gcaggtgggt gtcggatccg gtatcgagga cgagccgggt ggagaccccg
     8161 ccgatctcgg cctccaccat cggcgaatgg agcggcgtgc cgtcgggggc ggtgaaccgg
     8221 atcggtgcat ggaagggtcg gggatggtcg gccaccacgt cagtctagga tgccgccaat
     8281 gcgtgccacg cctgccgtcc ggttccggct gatggccgcg gctgtggtca tcggtctggt
     8341 cgtggccggg tgtgccctgc cggccaccga agaaggcttc aaccaggggt tctgcgaggt
     8401 tgatctcgcc ttcgagacgg ccgccgccgc gatcgtagag atcgaggatc gggaggagtg
     8461 gatcgacgcc acccgcgacg caatgctcga cttccgcgat gccctcaccc ggctgccggt
     8521 ttgggagccc gcgcgcgatg ccaagggtga gctggccaac gcgctgaatg ccatcgttcg
     8581 cctgatcgag agcggtgtcg ccggcgagct ggtggagagc gcgcagtggg agaacgcgct
     8641 cgagaccatg ctcaccgccc gggagaacct ccgcggggtg gcgggtccct gtatcagcca
     8701 gtagctaccg gcgccagatg ccgaccggct cgaaggtcat cgggacgtcg accaggatga
     8761 gctcgctgga cccggtcgcg tggatcgtga gctcagactc gtcagtgact ttcacggcgt
     8821 ccccggtgga caggtcctcc ccgttgaagt tggcggcgcc ctcgatcaag tagcagtaca
     8881 ccccccggcc gggaccgatc tggtggctga cctcgccgcc gggcgcgagc gacgaaacga
     8941 acaccgatgc gtcctggtgg accgccacca cgtcgccgcc accgggcccg atgaccggaa
     9001 ggaggcggtc agtccggtc
//
LOCUS       DASYGU010000243         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1684816, whole genome shotgun sequence.
ACCESSION   DASYGU010000243 DASYGU010000000
VERSION     DASYGU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684816"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2115
                     /locus_tag="VGO32_06880"
     CDS             136..2115
                     /locus_tag="VGO32_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV7200510.1"
                     /translation="MSLISLSGVSKSHGAQHLFSDVSLQVSAGRRIAIVGANGTGKTT
                     LLEIITGEQDADTGVVARGKDVVIGYLRQEVAESRGHTVLAEVLAGAGTVTGIGRRME
                     HIEREMAEATAQDELAELMDEYGRLQHRFEQLGGWSIEAEARRILAGLGFAHADAERD
                     IGEFSGGWMMRVALARLLLANPDLLLLDEPSNHLDLASVEWLQGFLAEYAGAVILVSH
                     DRDFINVVANRIAELHRGTLTEYVGDYADFVEQRDTRLAELERHARVQGREVARIERF
                     VERFRYKKSKARQAQAKIKYLERMERVVVPETRTRSIRFRFPQPARSGRTVISLDGIV
                     KRYGDHTVYDGLNLTLERGQKVALIGPNGAGKSTLLKILAGVLDYQAGERQLGHNVQV
                     AYFAQHQIEALNPRNTVLEELEEAAPRMTSGEQRRLLGTFLFSGDDIRKPVEVLSGGE
                     QTRLALAKMLADPANLLCLDEPTNHLDIASRDVLEDALTSFGGTLVLITHDRYLIRTV
                     ANTIVEVTGGDAVLYPDDFEAYAESRGIDIERPGASEGKATPRGVVSTPSRRETADQA
                     SGRKRTEAEARNRRHRETREARVALERTEADLAATEAALTDLTRRLGDPDTYADAALV
                     RRLIDEHNQALDRTATLTAERDRLTAELETAEAAG"
     gene            2112..3083
                     /locus_tag="VGO32_06885"
     CDS             2112..3083
                     /locus_tag="VGO32_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7200511.1"
                     /translation="MREARPSGDGLGASLVVLAAVCFATLGPTSRFAYAAGVDALTLV
                     TWRAVIGGLVVVVVALILARFGQVVARPFREIPRRQLGMNVFAGLTNALLNLTVLFAI
                     TRISVGLALLVFYTYPAMVAVVSTLLFKERLDGTRWAALGISFIGLILVLIGAGQVGQ
                     LDALGVALAFAGGLCQVVYALTARHGFPAVPSPQAGGVTFVVAATAYLLGAVVVGNVG
                     ALGAPLASSAALLPVLWAGIIGAGIPTMAWIMGIRLLGAPRAAIISTLEPVVGVLLAA
                     ILLSEVPTPLQIVGGAFIVVAAIVVQRRPGVEPAEHEAAPDGSILPS"
     gene            3080..3970
                     /locus_tag="VGO32_06890"
     CDS             3080..3970
                     /locus_tag="VGO32_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEV7200512.1"
                     /translation="MTVHRRMALSALAALVMAACTSAPSGAPSGSSTASVDPSAGAIA
                     SPEASGTPVATPAPTPTPAPTVPAGARLPNLIMEPLADWNVEYTEPDNRRLLHVTTLF
                     SNVGDGPFELRGSRMDPEAPAMVLDQVIYTEGGGFRRIPTRVAARYAGDGHNHWHAQQ
                     VVTMELAPVLDPSSVTTGNKIHFCFFDNSPTHRDAPGARGEAYYQNRWCGKPNSYSVR
                     MGLSVGWGDRYGWDFVGQYIDITNLAGGTYTLKATVDWSNNFYETDDTDNCTMSRIQI
                     PSAGEGPIIVVEATDLPCPT"
     gene            complement(3967..4989)
                     /locus_tag="VGO32_06895"
     CDS             complement(3967..4989)
                     /locus_tag="VGO32_06895"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV7200513.1"
                     /translation="MHSGPIVVDKFGGSVLTRPEDIAAAVEVVVAQQAMGLRPVVVVS
                     AFEGVTDAILSSAAVLLPDGGASLRGDAAHAPDSSMARETDRALATGEALSAALFALG
                     LEAHGIAARSFSGAEAGIRTAAAHLGAAVRRVYSRPLRTAVADGLVPVVAGFQGIGAH
                     GELTTLGRGGTDLSAVALAVALRADRCELFKDVGGVWEADPHLVPAARLLPAVSAIQL
                     ELLGELGAEVVHPVAVRRARRGRLRLVVRSLDGGAPATEVVPRPARDDPGALILAVDR
                     KERIARISLVGPAARMPELLPLPAHGEGSFDEGGLRVVWAEVPVGLAVQAAQAVHAGL
                     MARGPA"
     gene            5015..>5248
                     /locus_tag="VGO32_06900"
     CDS             5015..>5248
                     /locus_tag="VGO32_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV7200514.1"
                     /translation="MVQQYPRRVSSPPTTASIRAVRNRILAWFMADQRDLPFRRTRDP
                     WPVLVAEVMLQQTQASRIAERFDPFLHRYPSPAV"
BASE COUNT          792 a         1891 c         1812 g          753 t
ORIGIN      
        1 actctccgct caccgcgcac tcggtggaga aggcttggaa cggggccggc cagggaggga
       61 tggccacccg actccggttc gacctcgacg gggcgcggct gagctccgcc gagccgcgag
      121 agtgagcggc cgctcgtgag cctgatctcg ctgtcggggg tctccaagag ccacggcgcg
      181 cagcacctgt tcagtgacgt ctcgctgcag gtcagcgctg ggcggcggat cgccatcgtt
      241 ggagccaacg ggacgggcaa gacgaccctg ctcgagatca tcaccggcga gcaggacgcg
      301 gacaccggcg tcgtggcccg tggcaaggac gtcgtcatcg gctacctccg ccaggaggtc
      361 gccgagtcgc gcggccacac ggtgctggcc gaagtgctgg cgggcgccgg gacggtgacc
      421 ggcatcggcc gccgaatgga gcacatcgag cgggagatgg ccgaggccac cgcgcaggac
      481 gagctggccg agctgatgga cgagtacgga cggctccagc atcggttcga gcagctgggc
      541 gggtggtcca tcgaggccga ggcgcggcgg atcctggccg ggctgggctt tgcccatgcc
      601 gatgccgagc gtgacatcgg cgagttctcc ggcggctgga tgatgcgggt cgcgctggcc
      661 cggctgctgc tggccaaccc cgacctcctg ctcctggacg agccgtccaa ccaccttgac
      721 ctggcatcag tcgagtggct ccagggattc ctggccgagt acgcgggcgc ggtcatcctg
      781 gtcagccacg atcgggactt catcaacgtc gtcgccaacc ggatcgcgga gctccaccgg
      841 ggcaccttga ccgagtacgt gggcgactac gccgacttcg tcgagcagcg cgacacgcgg
      901 ttggccgagc tcgagcgcca tgcccgggtc cagggtcgcg aggtggcccg catcgagcgg
      961 ttcgtcgagc ggtttcgcta caagaagtcg aaggcccgtc aggcccaggc caagatcaag
     1021 tacctggagc gtatggagcg ggtggtcgtg cccgagaccc gcaccaggtc catccgcttc
     1081 cgttttccgc agccggcgcg cagcggacga acggtcatct ccctcgatgg catcgtgaaa
     1141 cggtacggcg accacaccgt gtacgacgga ctgaacctga ctctcgagcg tggccagaag
     1201 gtggcgctca tcggtcccaa tggcgccggc aagagcaccc tgctcaagat cctggccggt
     1261 gtcctcgact accaggccgg cgagcggcag ctgggccaca acgtgcaggt cgcgtacttc
     1321 gcgcagcacc agatcgaggc cctgaacccg cggaacacgg tgctcgagga gctcgaggag
     1381 gccgcccccc ggatgacctc gggggagcag cggcggctgc tgggcacatt cctgttctcg
     1441 ggggacgaca tccgcaagcc ggtcgaggtc ctgtccggcg gagagcagac ccggctggcg
     1501 ctggccaaga tgctggccga ccctgccaac ctcctgtgcc tggacgagcc gaccaaccac
     1561 ctcgacatcg ccagccggga cgtactggag gacgcgctga cgtcgttcgg cggcacgctg
     1621 gtcctgatca cccacgaccg gtacctgatc cgaaccgtgg ccaacacgat cgtcgaggtc
     1681 accggcgggg acgcggtgct ctatcccgat gatttcgagg cgtacgccga atcacggggc
     1741 atcgacatcg aacggccggg ggcgtcggag ggaaaggcca cgccgcgggg ggtggtgagc
     1801 acgcccagcc gtcgcgaaac agccgaccag gcctccggcc gcaagcgcac cgaggcagag
     1861 gctcgcaacc ggcgccaccg cgagacccgc gaggcccggg tcgcccttga gcgaaccgaa
     1921 gccgatctgg ccgccaccga ggcggcgctg accgatctga ccaggcggct cggtgacccc
     1981 gacacatacg ccgatgcggc gctcgtccgg cgcctgatcg acgagcacaa ccaggcgctc
     2041 gatcggacgg ccaccctcac cgccgagcgc gaccggctga ctgccgagct ggagaccgcc
     2101 gaggcggccg ggtgagagag gcgagaccct cgggcgacgg cctcggcgcg tcgctcgtcg
     2161 ttctggccgc ggtctgcttt gcgaccctgg gtccgacctc acgcttcgcg tacgccgccg
     2221 gcgtcgacgc gctgaccctg gtcacgtggc gggccgtcat cggcggcctg gtcgtggtcg
     2281 tggttgccct catcctggcc cgcttcggcc aggtcgtagc ccgaccattc cgcgagatcc
     2341 cgcgccgaca gctgggcatg aacgtcttcg cgggcctcac caatgcgctc ctcaacctga
     2401 ccgtgctgtt tgccatcacc cggatcagcg tcggcctggc gctgctggtc ttctacacct
     2461 acccggccat ggtggccgtg gtgtcgactc tcctgttcaa ggagcggctc gatggcacac
     2521 gctgggccgc gctcgggatc agcttcatcg gcctgatcct cgtgctcatc ggcgccggtc
     2581 aggtcggaca gctggacgcc ctcggggtgg cgctggcatt tgcgggtggt ctgtgccagg
     2641 tcgtctatgc cctcacggct cgccacgggt tccccgccgt gccatcgccg caggccggcg
     2701 gcgtgacctt cgtcgtcgcc gcgacggcgt acctgttggg cgcggtggtg gtcggcaacg
     2761 tcggggcgct gggtgccccg ctggcatcga gcgccgctct cctgccggtc ctgtgggcgg
     2821 ggatcatcgg cgccgggatt ccgaccatgg cctggatcat gggcatccgg ctgctgggcg
     2881 cgccgcgggc cgcgatcatc tcgaccctcg agccggtggt gggtgtcctc cttgccgcca
     2941 tcctgctttc ggaggtgccg acgcccctcc agatcgtggg cggggcattc atcgtggtcg
     3001 ccgccatcgt cgtccagaga cggccgggcg tcgagcccgc cgagcacgaa gcggcgcccg
     3061 atggctctat cctgccctcg tgactgtgca ccggcggatg gcgttgtcgg cgcttgccgc
     3121 actggtgatg gccgcctgca cctccgcccc aagtggcgcg ccgagcgggt cgtcgacggc
     3181 atcggttgac ccgtcggccg gcgcgattgc ctcgcctgaa gcgtccggca cgccagtcgc
     3241 gacccctgcg cccacgccca cgccagcgcc aaccgttccc gccggcgccc gtctgccgaa
     3301 cctgatcatg gagccgctgg ccgactggaa tgtcgagtac accgagcccg acaaccgccg
     3361 gctgctccac gtcacgaccc tgttcagcaa cgtgggcgac ggcccgttcg agctccgtgg
     3421 ctcgcgaatg gatcccgagg ccccggccat ggttctcgac caggtcatct acaccgaggg
     3481 cggcgggttc cgccgcattc cgacccgcgt cgcggcgcgg tacgcgggcg acgggcacaa
     3541 ccattggcat gcccagcagg tggtcaccat ggagctggcg ccggtgctcg atccctcctc
     3601 ggtcacgacc ggcaacaaga tccacttctg cttcttcgac aactccccga cccaccgcga
     3661 cgcgcccggc gcacggggcg aggcctacta ccagaaccgg tggtgcggca agccgaactc
     3721 gtacagcgtc cggatggggc tgtccgtggg ctggggcgac cggtatgggt gggatttcgt
     3781 gggccagtac atcgacatca ccaacctggc cggcggcacg tacaccctga aggccacggt
     3841 cgactggtcg aacaacttct acgagaccga tgacaccgac aactgcacga tgagccggat
     3901 ccagatcccg tccgccggcg aaggaccgat catcgtggtc gaggcaaccg acctcccctg
     3961 tccgacctag gccggtccgc gcgccatcag gccggcatgg accgcctgag ccgcctggac
     4021 ggcgagcccg accgggacct cggcccacac cacgcggagc ccgccctcgt cgaatgaccc
     4081 ttccccgtgg gcgggaagcg gcagcagctc gggcatccgg gccgcgggac caaccaggct
     4141 gatccgcgcg atccgctcct tgcgatccac ggccaggatc agcgctccgg gatcgtcacg
     4201 cgcgggacgg ggcacgacct cggtcgccgg cgccccgccg tccagggatc ggaccaccag
     4261 cctgagccgt ccgcggcgcg cgcgccggac ggcaaccggg tggacgacct ccgcgcccag
     4321 ctcgccgagc agctcgagct gaatggcgct gaccgcgggc agaagccgtg ccgcggggac
     4381 gaggtgcggg tccgcctccc acacaccgcc cacgtccttg aacagctcgc accgatcggc
     4441 gcggagggcc accgccaggg ccaccgccga caggtcggta ccgcctcggc cgagcgtggt
     4501 cagctcgcca tgggccccga ttccctggaa gccggccacc accggcacga gcccgtcggc
     4561 caccgccgtc cggagcggcc gcgaatagac ccgtcgcaca gcggccccca ggtgagcggc
     4621 cgcggtccgg atcccggcct cggctccgct gaacgagcgc gcggcgatgc catgggcttc
     4681 caacccgagc gcgaacaggg ccgcggacag ggcctcgccg gtggccagcg cgcgatcggt
     4741 ctcgcgggcc atggacgagt cgggggcgtg ggccgcgtcg ccgcgaagcg aggcgccgcc
     4801 gtccggaagg agcacggcgg ctgaagaaag aatggcatcg gtcacccctt cgaaggccga
     4861 caccaccacg accgggcgca ggcccatcgc ctgctgggcg acgacgacct cgaccgcggc
     4921 cgcgatgtcc tccggccggg tgaggaccga gccgccgaac ttgtcgacga cgatgggccc
     4981 gctgtgcatg gcgcggactc tagacgacca gccgatggtt cagcagtacc ctcgccgggt
     5041 gtcctcgcct cccaccaccg cctcgatccg cgcggttcgc aaccggattc tcgcctggtt
     5101 catggccgat cagcgtgacc tgccgtttcg ccggacgcgc gacccgtggc ccgtgctcgt
     5161 cgccgaggtg atgctccagc agacccaggc gtcgcgcatc gcggagcggt tcgatccgtt
     5221 cctgcaccgc tatccctcgc cggccgtg
//
LOCUS       DASYGU010000244         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1633452, whole genome shotgun sequence.
ACCESSION   DASYGU010000244 DASYGU010000000
VERSION     DASYGU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633452"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VGO32_06905"
     CDS             <1..1336
                     /locus_tag="VGO32_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7200515.1"
                     /translation="LKQLAAAAERLTLRAGEQLIREGDPAEELFVVVSGELEIRKQSG
                     KAEVALTRVGPGSIQGEMATFERSRRVASVYAVTETEVLRLPFESVRAMIAADPDVAT
                     ALLRTVTSRMRGIEETLRQREKLASLGTLAAGLAHELNNPAAAIRRSADELAEVTRER
                     NEAAVGIAVGHPQLAALVGAKPSEPDRDLGALDRADRTDAIAAALRTSGLAEDQGPDD
                     AAGRLTAAGWTPDDVTQALAGLEPAAVPAAVRWLSSVASGEELLSEIQMAAGRISAIV
                     GATKGYAYLDQAPVQRFAVTRGIDDTLVILEHKLGDIAVHRGYAPDLPEIEGWGSELN
                     QVWTNILDNAIDAMNGHGELTITVNPADAGGVHVSICDNGPGIPPEALPQIFEPFFTT
                     KPPGVGSGLGLHLSHNIVIQHGGTIELSSRPGSTCFEIGLPAILPTAAAKST"
     gene            1333..>1596
                     /locus_tag="VGO32_06910"
     CDS             1333..>1596
                     /locus_tag="VGO32_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200516.1"
                     /translation="MTSRTLTLGGTAYPLILPSIRDPRLHVASVILTIHLLGQTVLGF
                     QLTVPQIVAAMLTCAVIEVALTFRQTRSFVWPASAMLTGSGVAL"
BASE COUNT          238 a          577 c          561 g          220 t
ORIGIN      
        1 gctcaagcag ctggcggcgg ccgccgagcg gctcaccctt cgcgccggcg agcagctcat
       61 ccgggagggc gatccggccg aggagctctt cgtggtggtc agcggcgagc tcgagatccg
      121 caagcagtcg ggcaaggcgg aggtcgccct gacccgggtc gggccgggct cgatccaggg
      181 ggagatggcc accttcgagc gcagccggcg ggttgcatcg gtctatgccg tgaccgagac
      241 cgaggttctg cgcctgccgt tcgaatcggt ccgggccatg atcgcggcgg accccgacgt
      301 ggccaccgcc ctcctgcgga cggtcacctc gcggatgcgt ggtatcgagg agaccctccg
      361 gcagcgggag aagctcgcct cgctgggcac cctcgccgcg ggactggcgc acgagctcaa
      421 caacccggcg gccgcgatcc gccgctcggc ggacgagctg gccgaggtca cccgcgagcg
      481 gaacgaagcg gccgttggga tcgcggtggg ccatccccag ctcgccgcgc tggtcggggc
      541 caagccctcc gagccggacc gggatctcgg ggcgctggac cgggccgacc gaaccgatgc
      601 gatcgcggct gccctgcgca cctctggttt ggcggaggac cagggacccg atgacgccgc
      661 cggccggctg acggcggcgg gctggacgcc cgacgacgtc acgcaggccc tggccgggct
      721 cgagccagcc gccgttcccg ccgccgtgcg atggctgtcc agcgtggcct cgggggagga
      781 gctgctgagc gagatccaga tggccgccgg gcggatcagc gcgatcgtgg gcgcgaccaa
      841 gggctatgcc tacctcgacc aggcccccgt ccagcgcttc gcggtgacca gggggatcga
      901 cgacacgctg gtcatcctcg agcacaagct gggcgacatc gcggtgcatc gggggtacgc
      961 gcccgacctg cccgagatcg aaggctgggg gagcgagctg aaccaggtct ggaccaacat
     1021 cctggacaac gcgatcgatg ccatgaacgg gcacggcgag ctgaccatca ccgtgaaccc
     1081 ggccgatgcg ggcggggtcc acgtctcgat ctgcgacaac ggccccggca tcccgcccga
     1141 ggccctgcct cagatcttcg agccgttctt cacgaccaag ccgcccggcg tcggctcggg
     1201 gctcggactc cacctgagcc acaacatcgt catccagcac gggggcacaa tcgagctcag
     1261 ctcccgcccg ggcagcacgt gcttcgagat cggtcttccc gccatcctgc cgaccgccgc
     1321 tgccaagtcg acgtgaccag ccgaaccctg accctgggtg ggacggcgta ccccctgatc
     1381 ctgcccagca tccgcgaccc gcggctccac gtcgcgtcgg tgatcttgac catccacctg
     1441 ctaggccaga cggtgctggg cttccagctg accgtgcccc agatcgtggc cgcgatgctg
     1501 acctgcgcgg tgatcgaggt cgccctcacc ttccggcaga cgcgctcgtt cgtgtggccc
     1561 gccagcgcga tgctcacggg cagtggcgtg gccctg
//
LOCUS       DASYGU010000245         9692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_348634, whole genome shotgun sequence.
ACCESSION   DASYGU010000245 DASYGU010000000
VERSION     DASYGU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348634"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGO32_06915"
     CDS             <1..553
                     /locus_tag="VGO32_06915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV7200517.1"
                     /translation="PAETWPQQMVDRSGGTLILSGNLATNAFTSEDLILHQLPELDAV
                     RPELVSVLIGVNDVVQGVSEATYADNVAFILDDLLGRLPADRIVAVATPDYTVTSQGA
                     AFGDPDRMRAAIARFNTVFQLAAARRNIAFVPEIFEISQQAANDPSLVARDGLHPSGA
                     QYALWVDAILPVVESALAASAAP"
     gene            677..1513
                     /locus_tag="VGO32_06920"
     CDS             677..1513
                     /locus_tag="VGO32_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV7200518.1"
                     /translation="MTAEKPVLAQHGNAYNIFILVLTVFSLALMVLLLLPLSQPTRDL
                     LLVYDNVVCGIFLVDFVMNLFGSNPKRAYFIGARGWLDLLGSLPSLGFFQIGALLRLA
                     RLSRLARITRLLRGQAGKDLVRDVLMNRSQYATFITILLAGMTLSIATILVVQFESRS
                     PDSNITTGGDALWWGIVTMTTVGYGDFFPVTTAGRFTGAFVMFAGIGIIGALASILAS
                     LLVSSPSPAEPEADASSAAATGDSASSGALLEELAGIRTEVAGQRAEIAALRAALRNG
                     GT"
     gene            1551..3056
                     /gene="gltX"
                     /locus_tag="VGO32_06925"
     CDS             1551..3056
                     /gene="gltX"
                     /locus_tag="VGO32_06925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV7200519.1"
                     /translation="MSSPPDDRPIRVRMAPSPTGPLHIGTARTSLYNALFARHVGGTY
                     VLRIEDTDLARSTADFERDILDNLHWLGITWDEGPQHAGGDAGGEDIGPYAPYRQSLR
                     MDRYAREADRLLASGAAYHCWCTTDELDAVRREQEARKQAPRYNGRCLTLTDADRAQF
                     EAEGRRPAVRFRVPAERIRFDDLIRGEVEFDNGLLGDFVIVRADGVPLYHFVVVIDDE
                     QMEISHVIRGEDHLSNTPKHIALIRALGYREPVFGHIPLILNPDRSKMSKRKSQTAIS
                     DYREQGYLPEAMVNFLAFLGWSPGTKEEIFSLDELAARFEIGEVHKAGAVFDRDRLDY
                     LNGVYIRGLTDDELTARLRPLLPAALADHVLAGLVPLVKERMVRLTDAVELSDFLSEP
                     DEVVASWWSVDDLLPKGRDATEVAAALGAARAALAADDGWAAERLETVGREAAEAHGW
                     KAGDFFKPIRVAVTGKAISPPLFGSMVLLGRERSLARMDGALERLRTGAAA"
     gene            3053..6256
                     /locus_tag="VGO32_06930"
     CDS             3053..6256
                     /locus_tag="VGO32_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7200520.1"
                     /translation="MTGDDPTLNCPACGAANQPGSRFCSSCATPLGAAAEPAAETRKT
                     VTVLFCDATSSTALGERLDPESLRSVMTRYFDVMREVIEFHGGVVEKFIGDAVMAVFG
                     VPTIHEDDALRACRAAIEIHDRLGELDEAIRSERGASVEWRMGINTGEVVAGDAVSGQ
                     RIVTGDAVNVAARLEGAASPGEILLGADTHALVREDVTAEPVEALSLKGKAEPVPAWR
                     LTAVGGSIGRRVRPLEAPLVGRTRPLRLLEEAFREAVEERVCHLLTILGVAGVGKSRL
                     VDEFVGSLGEQAQVATGRCLAYGHGITYWPVTEAIRGGAAIHEDAAPDVALARIREVL
                     SHEPEADRVVPVIGHLLGATEALPAPDEMFWAIRKTFEAMARDRPLVLVFDDIHWGEP
                     TFLDLIDHVADWTRDAPILLIAMARPDLLEMRPTWGGGKRWVTTMQLEALSADESNEL
                     VGGLLGQADLPADVRAQIGQAAEGNPLFVEELLGKLIDDGFLVQSGDGWAAHGNVSEL
                     AIPPTIHALLAARLDGLASEDRAVIERASVEGKIFHRGAVTELAPELMRPHVRERLAG
                     LMRMELVRPDQASFAGDEAYRFRHLLIRDAAYQALAKQTRSELHERFAAWLERVAADR
                     LSEYEEIIAYHVEQAYRYRVELGPPDAHARELALRAGTQLADAGERADARSDVAAAVD
                     LLGRAVDLLNDDLPRRRRVLITLADNCYNAGDGPRAERLLADAIADAEQADDEGAAAL
                     AAVALVMIQMSTKSTEMSVVLRELERLIAVLERLGATADARLAEAWSAFCLFGVGRAG
                     EAAERARSLVELGEGGERWQREARMAMGVSLVFGPTPADEVIAELQAVRERAGTKSLA
                     EPPFRGIGRMKLLQGRIDEAHELNARVQAVCEEIGNRQQLASCLDFEGQVQRLAGNLD
                     EAARLSREGYQAMAAIGDRSFASTIAVNLGEILIDLGQDDEAWRFGAIARDTSSSDDV
                     VSQAGGRAVQARVLSRRGEHAVAEGLAREAADIMSNTDYFDQHAGVLVHLAHVLREAD
                     KSGDALVAAREALSLYELKGATFFVHQSQRLIDEWVG"
     gene            6256..6384
                     /locus_tag="VGO32_06935"
     CDS             6256..6384
                     /locus_tag="VGO32_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200521.1"
                     /translation="MADQEDEAVAELGMALELFERKGHLAGAEKARISVLPGDGAL"
     gene            complement(6381..7403)
                     /locus_tag="VGO32_06940"
     CDS             complement(6381..7403)
                     /locus_tag="VGO32_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV7200522.1"
                     /translation="MLAVRTYAAGEPMRLEDVPVPTPRGAEVLLRVAGAGVCHTDLHI
                     VRTDRIRVERPVTLGHEVAGWIAGMGADAGGLLRRARLKEDEPVAVFGGWGCGECRDC
                     RRGDEQRCEQSRAPGFQVDGGYAEYLLVPHPRHLVSLGKLDPVEAAPLADAGVTSFRA
                     VRRAAPWLAEGSRALLIGLGGVGQFALQFLRRLPDVFIGVREIDPDKVQIAADLGADV
                     GFLLGEEELIFAGLGGHADVVFDFVGSDETLALAARFVAPGGLVSLVGEAGGALTFSF
                     AALPPEASLTTTAWGSHDDLRDVVQLARRHSLHWSVERMPLAEAAAAHDRLAAGKVPG
                     RVVLVP"
     gene            complement(7407..7913)
                     /locus_tag="VGO32_06945"
     CDS             complement(7407..7913)
                     /locus_tag="VGO32_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV7200523.1"
                     /translation="MSRTTASLKIQVGDPIPSIGLRATDGYLLNLRSWVGKSPVAHVF
                     FAGPSLSGAGRLAGEVLARTLAAEAARLTEAGIDVAGVTTDNEREQAAFARDLSLPFL
                     LLSDERQIASEALGVPLVQRRGNTNVATPVLIGADEAGLVRGVYHDPDPRLVAAIIFE
                     IFREPLPA"
     gene            complement(7969..8148)
                     /locus_tag="VGO32_06950"
     CDS             complement(7969..8148)
                     /locus_tag="VGO32_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200524.1"
                     /translation="MTAWELEADRRRELAAPYRPAHRPIVVAARRDGPAASDRRLLFG
                     WRLAPRLRRWEDASS"
     gene            8330..8893
                     /locus_tag="VGO32_06955"
     CDS             8330..8893
                     /locus_tag="VGO32_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7200525.1"
                     /translation="MTTPADERDRLDDMLLVWAREIPNLDPLTEGIVERIQFLNKRFG
                     ESMDATLEQFGLEPRSFHLLGKLRSYGPPYQRSPGQLAADMHLSSGAMTNRLDRMEEA
                     GLIRRLPDPNDRRGTLIEPTEAGHAAWDKTVGTQAVTEALIAGTLSAAERKELHRLLR
                     RLMRAFPAKDHWAEAPAVEKPEAVAVS"
     gene            complement(8898..>9692)
                     /locus_tag="VGO32_06960"
     CDS             complement(8898..>9692)
                     /locus_tag="VGO32_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV7200526.1"
                     /translation="RTRDPWPVLVAEVMLQQTQASRIAERFDPFLHRYPSPAVLSATP
                     VARVLADWSGLGYNRRAVLLHRTATAIAADGWPTSVAGLEALPGIGAYSARAIAAIAF
                     GQPVGAVDTNVRRWLVRRFGVGAADRRGLQLLADRLAEQASADDPLEPGTWTHATMEF
                     GARICLARTPRCDICPVSRGCPSRTDPQRVPVPRQSPATASTRAARGALLRALADAPG
                     HRISQATAGRSVVNLTPADYEALSAGLERDGLVHRSGKVLILGPRA"
BASE COUNT         1510 a         3314 c         3418 g         1450 t
ORIGIN      
        1 cccggcagag acctggcccc agcagatggt cgaccgcagc ggcgggaccc tcatcctgtc
       61 cggcaacctg gcgaccaatg cgttcaccag cgaggacctc atcctccacc agctcccaga
      121 gctcgatgcg gtgcgtcccg aactcgtgtc ggtcctgatc ggggtcaacg acgtcgtcca
      181 gggcgtgtcg gaggcgacct acgccgacaa cgtcgcgttc atcctggacg acctgctcgg
      241 ccggctgccg gccgaccgga tcgtggccgt cgccactccg gactacacgg tcacgtcgca
      301 gggagccgcg ttcggggacc cggaccggat gcgggcggcg atcgcccgct tcaacaccgt
      361 gttccagctg gccgccgcgc ggcgcaacat cgcgttcgtg ccggagatct tcgagatctc
      421 gcagcaggcc gccaacgacc cgtcgctggt ggcccgcgac gggctgcatc ccagcggcgc
      481 gcagtacgcc ctgtgggtcg acgccatcct cccggtcgtc gagtccgcac tggcggcgag
      541 cgcggctccc tagcgccgga ccgcgcccgg tacccgggtt ccggcgcgag tgggcgctac
      601 catccagcga ccgatggccg cgctcggatg gcgcccagcc cgaccgggtc acacgagttc
      661 tggaggtcac caccgcatga ctgccgagaa gccggtgctg gctcagcacg gcaacgccta
      721 caacatcttc atcctggtcc tgaccgtctt ctcgctggcg ctcatggtcc tcctgcttct
      781 gccactgagc cagccaacgc gcgacctgct ccttgtctac gacaacgtcg tgtgcggcat
      841 cttcctcgtg gacttcgtta tgaatctgtt tggctcgaat ccaaaacgcg cctatttcat
      901 cggcgcgcgg ggctggctgg acctcctggg ctcgctgccg agcctcggct tcttccagat
      961 cggcgcgctg ctgcgattgg ccaggctcag tcggttggcc cgcatcaccc gcctgctgcg
     1021 cggccaggcc ggcaaggatc tcgtccggga cgtgctcatg aaccggagcc agtacgcgac
     1081 cttcatcacg atcctgctgg cgggcatgac cctatcgatc gcgacgatcc tggtcgtcca
     1141 gttcgagagc cggtcgcccg attcgaacat cacaacgggt ggtgacgcgc tgtggtgggg
     1201 catcgtcacg atgaccacgg tcggctacgg cgacttcttc ccggtgacga ctgccggccg
     1261 gttcaccggg gccttcgtca tgttcgcggg gatcgggatc atcggcgccc tggccagcat
     1321 cctggccagc ctgctcgtct cgtcgccgtc gccggcggag ccggaagcag atgcctcttc
     1381 cgctgccgcg accggcgact cggcgtcgtc cggcgcactc ctcgaggagc tggcgggcat
     1441 ccggaccgag gtggccgggc agcgagccga gatcgcggcc ctccgcgccg cgctccgcaa
     1501 cggcgggacc tgaccatttc cgagcgggtc ggcgctacca tcgccagacc atgagctcac
     1561 cgcctgacga tcggcccatt cgcgtccgga tggcgcccag ccccaccggg ccgctccaca
     1621 tcgggacggc ccgaaccagc ctgtacaacg ccctgttcgc gcgccacgtc ggcggcacgt
     1681 acgttttgcg catcgaggac accgatctcg cccgcagcac ggccgacttc gagcgcgaca
     1741 tcctcgacaa cctccactgg ctggggatca cctgggacga ggggccgcag cacgccggcg
     1801 gtgacgccgg cggcgaggac atcggtccct atgcgcccta ccgccagagc ctgcggatgg
     1861 accgatacgc ccgcgaggcg gaccgtctcc tggcctccgg cgccgcgtac cactgctggt
     1921 gcacgacgga tgagctggac gccgtccgcc gtgagcagga ggcccgcaag caggcgcccc
     1981 gctacaacgg ccgctgcctg accctgaccg atgccgatcg ggcgcagttc gaggccgagg
     2041 ggcgtcgccc cgcagtccgc ttccgcgttc cggccgagag gatccgcttc gacgacctca
     2101 tccggggcga ggtggagttc gacaacggat tgctcggcga tttcgtcatc gtccgggccg
     2161 acggtgtgcc gctctaccac ttcgtggtcg tgatcgacga cgagcagatg gagatcagcc
     2221 acgtcatccg cggcgaggac cacctgtcga acacgcccaa gcacatcgcc ctgatccgag
     2281 cgctgggcta ccgcgagccg gtgttcgggc acatcccgct catcctcaac cccgaccggt
     2341 cgaagatgag caagcgcaag tcacagaccg cgatcagcga ctaccgggag cagggctacc
     2401 tgccggaggc gatggtcaac ttcctcgcgt tcctcggctg gtcgccgggg acgaaggaag
     2461 agatcttttc cctcgacgag ctggccgcgc ggttcgagat cggcgaggtc cacaaggcgg
     2521 gcgccgtgtt cgatcgcgat cggctggact atctcaatgg ggtgtacatc cggggcctga
     2581 ccgatgacga gctgacggcg cgtcttcgac cgctgctgcc ggccgcgctg gccgaccatg
     2641 tcctcgccgg gctcgtgccg ctggtcaagg agcggatggt gcgcctgacc gatgcggtcg
     2701 agctgtccga cttcctgtcc gagcccgatg aggtggtggc ctcatggtgg agcgtggacg
     2761 acctgttgcc gaaaggtcgg gacgcgaccg aggtcgccgc ggcgctcggt gcggcccgcg
     2821 ccgcgctggc cgccgacgac gggtgggccg cggagcggct ggagaccgtg ggtcgcgagg
     2881 ccgctgaagc gcatggctgg aaggcgggcg acttcttcaa gcccatccgg gtggcggtga
     2941 ctgggaaggc catctcgccg ccgctgttcg ggtcgatggt cctgctgggt cgcgagcggt
     3001 cactggcgcg aatggacgga gcgctcgagc gcctccgcac gggagcagca gcgtgaccgg
     3061 cgacgacccg acgctgaact gccccgcctg cggggcagcc aaccagccgg gatcgcgctt
     3121 ttgctcgtcg tgcgccacgc ccctgggtgc ggcggccgag ccggccgccg agacgcgcaa
     3181 gacggtcacg gtgttgttct gcgatgcgac gtcgtctacc gcactcgggg agaggctcga
     3241 tccggagtcc ctgcgatcgg tgatgacccg ctacttcgac gtgatgcgcg aggtgatcga
     3301 gttccatggt ggcgtcgtcg agaagttcat cggggatgcg gtgatggccg ttttcggcgt
     3361 gccgacgatc cacgaagacg acgcgctccg ggcgtgtcgg gcggcgatcg agatccacga
     3421 ccgcctcggc gagctggacg aagccatccg ctccgagcgc ggtgccagcg tggagtggcg
     3481 gatggggatc aacacggggg aagtggtggc cggcgacgcg gtgagcggtc agcggatcgt
     3541 gaccggcgac gcggtgaacg tggccgcgcg gctggaaggt gcggcgtcgc ctggggagat
     3601 cctgcttggt gccgacaccc acgccctagt acgtgaggac gtgacggccg agccggtgga
     3661 ggcgctgtcc ctgaaaggta aggcggagcc cgtgcccgcc tggcggctga ccgcggtggg
     3721 tgggtccatc ggccgtcgcg tgcggccgct cgaagctccg ctcgtcgggc gcacgcgacc
     3781 gttgcgactc ctcgaggagg ccttccgcga ggccgttgag gagcgtgtct gccacctcct
     3841 caccatcctg ggcgtggcgg gagtgggcaa gtcgcgcctc gtggacgagt tcgttggctc
     3901 gcttggcgag caggcacagg tggcgaccgg ccgctgcctc gcctacggcc acgggatcac
     3961 ttactggccc gtgaccgaag ccatacgcgg cggcgccgcc atccacgagg atgccgcgcc
     4021 cgacgtcgcg ttggcacgca ttcgcgaggt cctgtcccat gagccggaag ccgaccgagt
     4081 ggtgcccgtg atcggtcacc tgctcggcgc gaccgaagcc ctgccggcgc cggatgagat
     4141 gttctgggct attcggaaga cgttcgaggc catggcccgg gatcggcctc tggtgctcgt
     4201 cttcgacgac attcattggg gcgaaccgac attcttggac ctcatcgacc acgtcgctga
     4261 ctggacacgc gacgcgccca tcctgcttat cgccatggcc cggcctgacc tactcgagat
     4321 gcgtccgacc tggggcgggg gcaagcggtg ggtgacgacg atgcagctcg aggcgctgtc
     4381 cgcggatgaa agcaacgagc tggtcggtgg ccttctgggt caggccgacc tgccggccga
     4441 cgtgcgggcg cagatcggtc aggcggccga aggcaacccg cttttcgtgg aggagctgct
     4501 cggcaagctc atcgacgacg gattcctcgt ccagtcaggc gacggttggg cagctcatgg
     4561 caacgtgagc gagctggcca ttcctccgac catccatgcc ctcctcgccg ctcggctgga
     4621 tggattggcc agcgaggacc gcgctgtcat cgagcgagca tctgttgaag gcaagatctt
     4681 ccaccgcggg gcggtgaccg agctggcccc tgaactcatg cgcccccacg tccgggagcg
     4741 cctggccggg ctgatgcgga tggagctggt ccgcccggac caggcctcct tcgcgggaga
     4801 tgaggcgtac cgcttccgcc atctgctcat ccgcgacgcc gcgtaccaag cgcttgccaa
     4861 gcagacccga tcggagctgc acgagaggtt tgctgcctgg ctggagcgag tcgcggcaga
     4921 tcggctgtcg gaatacgagg agatcatcgc ctaccacgtc gagcaggcgt accggtatcg
     4981 agtcgagctg ggcccgcccg atgcccacgc cagagagctc gccctgcggg cgggaaccca
     5041 actggccgat gccggagagc gggccgatgc ccgctccgac gtcgcggcgg cggtcgacct
     5101 gctcggccgt gccgttgacc tgctgaacga cgaccttccc cgccgccgac gcgtgctcat
     5161 cactctcgct gacaactgtt acaacgccgg cgatggaccg cgggcggaac gcttgctggc
     5221 cgatgccatc gccgatgcgg aacaagccga tgacgaaggc gcggcggcgc tggctgccgt
     5281 ggcattggtc atgatccaga tgtcgaccaa gtcgacggag atgtcggttg tcctccgcga
     5341 attggagcgc ctgatcgccg tcttggaacg gctcggcgcg accgccgatg cccggctcgc
     5401 cgaggcctgg tcggcgttct gcctgttcgg cgtcggccgg gcgggggagg ctgcggaacg
     5461 agcgcgatct ctcgtcgagt tgggcgaggg cggcgaacga tggcaacgcg aggcccggat
     5521 ggccatgggc gtgtccctgg tattcggccc aacgccggcg gacgaggtca tcgccgagct
     5581 gcaggcggtc agggagcgtg ccgggacgaa gagcctggcg gagcctccct tccgaggaat
     5641 tggtcggatg aagctgctcc agggccgcat tgatgaggcg cacgagctga atgcccgcgt
     5701 gcaggcagta tgtgaggaga ttggaaaccg gcagcagctg gcctcgtgcc tcgatttcga
     5761 ggggcaggtc caacgcctgg ccgggaacct ggatgaggcg gcgaggttat cccgtgaggg
     5821 gtatcaggct atggcggcca ttggtgaccg gtcgttcgct tcgacgattg ccgtcaacct
     5881 gggcgagatc ctcatcgacc tcggccagga cgacgaggcg tggcggttcg gtgcaatcgc
     5941 cagggacacc tcctcgagcg acgatgtggt ctcccaggcc gggggccgtg cggtgcaggc
     6001 gcgagtcctg tcgcgacgtg gcgagcacgc cgtggccgaa ggtctcgccc gcgaggcggc
     6061 tgacatcatg agcaacaccg actacttcga ccagcacgcc ggagtcctcg tccatctggc
     6121 gcacgtactc cgcgaggccg acaagagcgg ggatgcactc gtcgcagccc gtgaagcgct
     6181 cagcttgtac gagttgaaag gcgccacgtt cttcgtgcac caatcccagc gcctgatcga
     6241 cgagtgggtc ggctgatggc cgaccaggag gatgaagcgg tcgcggagct ggggatggct
     6301 ctcgagctgt tcgagcgaaa gggccacctg gcgggtgctg agaaggcacg catctccgtg
     6361 ctcccgggcg acggggcact ttaggggacg agcaccaccc ttccaggcac tttgcccgct
     6421 gccagacggt cgtgagcggc tgcggcctcg gccagcggca tccgttccac cgaccagtgc
     6481 aggctgtgtc ggcgcgcaag ctggacgacg tcgcgcaggt catcgtgcga gccccacgcg
     6541 gtagtcgtga gcgatgcctc tggcgggagg gcggcgaacg agaaggtcag cgctccgccc
     6601 gcctcgccga cgagcgacac gaggccgccg ggcgcgacga accgcgcggc gagggcgagc
     6661 gtctcgtccg accccacgaa gtcgaagacc acgtcggcgt ggccgcccag gccggcgaat
     6721 atcagctcct cctcgcccag caggaagccg acgtccgcgc ccaggtcggc ggcgatctga
     6781 accttgtcgg ggtcgatctc gcgcactccg atgaacacgt cgggaagacg gcggaggaac
     6841 tggagcgcga actggcccac gccgccgagg ccgatgagca gggcgcgcga tccctcggcc
     6901 agccacgggg ccgcccggcg gaccgcccgg aacgaggtca cgcctgcatc cgccaaggga
     6961 gcggcctcga ccggatcgag cttgcccagc gagacgaggt gccgggggtg ggggacgagc
     7021 aggtactccg catagccgcc gtccacctgg aaccccggag cgcgggattg ctcgcaacgc
     7081 tgttcgtcac cgcgccggca gtcacggcac tcgccgcatc cccacccgcc gaagaccgcg
     7141 accggctcgt cctccttcag gcgcgcccgg cggaggaggc caccggcgtc cgcgcccatg
     7201 cccgcgatcc aaccggccac ctcgtgaccc agggtcaccg gccgctcgac ccggatccga
     7261 tcggtccgga cgatgtggag atcggtgtgg cacacgcccg cgccggcgac ccggaggagg
     7321 acctccgcgc cgcggggcgt gggaacgggc acgtcctcca ggcgcatcgg ctcgccggcg
     7381 gcatacgtcc ggacggccag catcggtcag gccgggaggg gctctcggaa gatctcgaaa
     7441 atgatggcgg ccacgagccg gggatcggga tcgtgataga ccccgcgaac gagccccgcc
     7501 tcatcggctc cgatgaggac cggcgtcgcg acgttcgtat tgccccgccg ctgcacgagg
     7561 gggacgccga gcgcttcgga ggcgatctgg cgctcatcgc tgagcaggag gaacggcagg
     7621 ctcaggtcgc gggcgaaggc agcctgctca cgctcgttgt cggtagtgac gcccgccacg
     7681 tcgatacccg cctcggtcag gcgcgcggct tccgccgcca gggtgcgggc cagcacctca
     7741 ccggccaagc ggccggcgcc actcaagctc ggcccagcga agaagacgtg cgccacgggc
     7801 gacttcccga cccacgatcg gagattcagg agatatccgt cggtcgcgcg gagaccgatg
     7861 gacgggatcg ggtcaccgac ctggatcttc agcgaggcgg tggtgcggct cacgccgcga
     7921 ttgtatccgg gctcgcgatc cggcccggcc cgaaggccgg gccatcggtc aggagctcgc
     7981 gtcctcccag cggcgcagcc gcggggcaag ccgccagccg aacagcaggc ggcgatcgga
     8041 cgcggcaggt ccgtcgcggc gagcggcgac cacgatcggc cggtgcgcgg gccgatacgg
     8101 tgccgccagc tcgcggcggc ggtcggcctc gagctcccag gccgtcatcg gggtgtttcc
     8161 aaacacaggt tgagctcctt tccctgcccg ctcccgcggg acgaatgtct gcatctgaag
     8221 aggtttgata tcgaacgagt tcggagagta gcgcggtcat cgtttgatgt caagcaatac
     8281 gatgacaaaa ggcttgacat ggaactaaca tgacaccaat actgtcgtca tgacgacacc
     8341 cgcagacgaa cgggaccgcc ttgacgacat gcttctcgtc tgggcgcgcg agatcccgaa
     8401 cctggatccg ctgacggagg gcatcgtcga gcgcatccag ttcctcaaca agcgcttcgg
     8461 cgagtccatg gacgcgacgc tcgagcagtt cggcctcgaa ccgcggtcct tccacctcct
     8521 gggcaagctg cgcagctacg ggccgcccta ccagcggtct cccggtcagc tcgccgcgga
     8581 catgcacctg tcgagcgggg cgatgacgaa ccgtctcgac cggatggagg aggcagggct
     8641 catccgccga ttgccggatc cgaacgaccg gcggggcacg ctcatcgagc ccactgaggc
     8701 gggccacgcc gcgtgggaca agacggtcgg cacccaggcg gtcaccgagg cgctgattgc
     8761 cggcaccctg agcgcggccg agcgcaagga gctccaccgg ctcctgcggc gcctgatgcg
     8821 cgcgttcccg gcgaaggacc actgggccga ggcgccggcc gtcgagaagc ctgaggcggt
     8881 cgccgtttcc tgaccggcta cgccctcgga ccgaggatga gcaccttccc ggaccgatgg
     8941 accagcccgt cgcgttccaa tccggcgcta agcgcctcgt aatcggctgg ggtcaggttc
     9001 accacggatc gtcctgcagt cgcctgactg atccggtgcc cgggcgcgtc ggccagggcg
     9061 cggagcagcg caccgcgcgc ggcccgcgtg ctggcggtcg ccggcgattg gcgcgggacc
     9121 ggaacccgct gggggtcggt ccgggacggg cagccgcggc tgaccgggca gatgtcgcat
     9181 cgcggcgtgc gcgccaggca gatccgagcc ccgaactcca tcgtggcgtg ggtccaggtt
     9241 cccggctcga ggggatcgtc ggccgaggcc tgttccgcca gtcgatcagc caggagctga
     9301 aggccacgcc gatccgccgc acccaccccg aaccggcgca cgagccaccg ccgcacgttg
     9361 gtgtccaccg ccccgaccgg ctgcccgaac gcgatggccg cgatcgcgcg ggcggagtag
     9421 gcgccgattc cgggcagggc ctcgaggccg gccaccgagg tcggccaccc gtcggcggcg
     9481 atggccgtcg cggtccggtg cagcagcacc gcgcggcggt tgtagcccag gccgctccag
     9541 tcggcgagga cccgcgccac cggcgtggcg gacaacacgg ccggcgaggg atagcggtgc
     9601 aggaacggat cgaaccgctc cgcgatgcgc gacgcctggg tctgctggag catcacctcg
     9661 gcgacgagca cgggccacgg gtcgcgcgtc cg
//
LOCUS       DASYGU010000246        13196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1839917, whole genome shotgun sequence.
ACCESSION   DASYGU010000246 DASYGU010000000
VERSION     DASYGU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839917"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="VGO32_06965"
     CDS             <1..1569
                     /locus_tag="VGO32_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEV7200527.1"
                     /translation="WDALRERIFARQKEMGVIPADAELTARPAEIPAWDDMPDAMKPV
                     LARQMEVYAGFMEHTDHHVGRLIDALADLEILDDTLVYYIVGDNGASAEGTPNGTFNE
                     LMTLNGVTTIETPEFLTARIDKFGTPEAYNHYAVGWAHAMDTPYQWTKQVASHWGGTR
                     NGTIIHWPNGFQARGGIRHQFSHVIDIAATVLDAAGLPEPTFVHGVQQMRLHGRSMRP
                     TFDDEAAPEHRATQYFEMFVNRGIYHRGWTAVTRHSTPWIMAEAPPIDADVWELYAPD
                     DWTQAHDLAAEQPEKLAYLQRLFLIEATKYDVLPLDDRRVERFNSDLAGRPTLIHGNS
                     QVLFDGMGRLSENSVLNIKNKSHAVTAQVTIPDGAAANGVIVAQGGAFGGWAVYAHAG
                     KPAYCYNLFGLQRFKIYGDRDLPAGDHQVRVEFGYDGGGLAKGGSVSLYLDGEKVGEG
                     RVDQTEPMAFSGDETTDVGSDTATPVSDDYSSPSSVFNGRIQWVQIDLGDDAEDADHL
                     ITAEDRFRVAMARQ"
     gene            complement(1592..2032)
                     /locus_tag="VGO32_06970"
     CDS             complement(1592..2032)
                     /locus_tag="VGO32_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV7200528.1"
                     /translation="MTAVPGTAVTRARPPARRPSFRVGLRRRTFVALIVAGVVAIIAF
                     NVGRQAFLGWSVGEQADSLAAQVAAAEAENAALQRQLEYLQSDAYVTVEARRLRNLGL
                     PGEQVLIIPPGAAVSAPMPTRPVTAPDPPMLERWIEVLVGSDSP"
     gene            2238..2681
                     /locus_tag="VGO32_06975"
     CDS             2238..2681
                     /locus_tag="VGO32_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEV7200529.1"
                     /translation="MGIGPVQYMVVAFPGNQFKGEIAPALSDLVESGTIRIIDLAFVI
                     KEADGSMTGVELEDVGSDVFQAFESLTAERGGFVSEEDLQAVAGALEPNSSAAILVWE
                     DVWATGFVEAIARADGQLVDIQRVPRELVEEAVAWAEENKATASA"
     gene            2709..2996
                     /locus_tag="VGO32_06980"
     CDS             2709..2996
                     /locus_tag="VGO32_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV7200530.1"
                     /translation="MRRRRGGIVRMAATTAVVAGTAGAVSHHQQQKYAAQDQQQAAQQ
                     QAGYDQAAAAAPPAPAAEDPMVEIQKLAQLHSAGALTDEEFAAAKAKVLGI"
     gene            complement(3115..4428)
                     /locus_tag="VGO32_06985"
     CDS             complement(3115..4428)
                     /locus_tag="VGO32_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7200531.1"
                     /translation="MTWLLAVAGAFLVYAVLSRRFAASVVSAPIYFLGIGAILGFVGV
                     LPIEPGDVPLRTLAEVTLTVVLFADASSIHLGQLRAGWKIPARLLGIGLVLTIALGTV
                     VAIPLFPGLLVAEALVLAVCLAPTDAALGLPVVLDERVPARIRQALNVESGLNDGICV
                     PILFTAVALALAEGDAVTVGDALATLVRQLGFGVAAGFAVGALGAAAARIGRRSGSMQ
                     EAWAQVIPLTAAVAAYALAVSLGGSGFIAAFVGGAVFGIISRRAARADHDEAAAEGHP
                     NELLEVIGGLLAAGTFLAFGAGVLVPIVSEIRAEHVVYALLSLTVIRMLPVAVSLVGS
                     GARPATVGFIGWFGPRGLASIVFAIIVLEELAAKPGALDITVTVAITVVLSVVAHGMS
                     AWPLAGRYGAWFARVSAESPDLMEGTPVSAVRSRGGVFGSPTRTE"
     gene            complement(4483..6072)
                     /locus_tag="VGO32_06990"
     CDS             complement(4483..6072)
                     /locus_tag="VGO32_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7200532.1"
                     /translation="MTDRYDAIVVGGGHNGLVNGAYLAKGGLRTLILEQRPFVGGAAI
                     TEELRPGFWFTTFSYALSLLRPQIVQELELPKHGFLPLLMSSRFAPTPEGEYLWFTQD
                     HDANLREIARISPHDADAYEQYGHDMDRVAQVLKPLLDMVPPDPFSDDPEELVALAAL
                     GAGFRKLDKRTLHNAIRLLTGSAADFLDDYFESDLLKGWLASSGIIGSKVGPRSQGSG
                     LVLIFHSLGEHDGSFGTWAFHKGGNGGFTQVLARAAQAYGAEIRLDSEVARVLTDDGR
                     ATGVALADGTEFHADVVVSALDPRRTFLDLVEPRELPDDLTETVRRMKFQGVSSKVNF
                     ALSGIPVYPQLADAGTEPFRGFLNIGPSMDYLERAFDEAKYGWYSAEPYIDGAIQSTI
                     DPDMAPPGQHVMSCFVQYTPYRLRESDWDAERQNLGNVVQATLERYFPGFGDLVLQRE
                     VVTPLDIERVTGLSEGNIFAGELLAPQMFFLRPAPGWAQYRTPIHGYYQCGSGTHPGG
                     CVMGAPGRLAAGQILKDRGTA"
     gene            complement(6069..7268)
                     /locus_tag="VGO32_06995"
     CDS             complement(6069..7268)
                     /locus_tag="VGO32_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEV7200533.1"
                     /translation="MIRTTPFHERTSALNETGLWSHWAGHLAANRYQVSDKFEYFAVR
                     NAAGIFDTSPLYKYRIAGRDAERFLAGMLARDIRACPSSHAQYTTWLDDRGFVFEDAV
                     IQRRGPDEYLLTTAEPNYAYFADRIGHMAVTVEDVSLDIGALAVQGPRSRELLARLVP
                     AVAEVPFFGITTGEIGGRPVTVTRTGYTGDLGYEVWCTTPDALTVWDTLWEACDGYGV
                     QAYGLAALYMLRIEAGLLLLGKDFDSSRFAFNDAHRSTPLELGWAWMFKGLADDDRAF
                     IGRKALEREIADRSSRWRMSGLVVDWEDYDRVYGAAGLIPPKAHAPVHEDWMVYDEGS
                     QRVGYATSFMYSPVLQRHIAMARVRPDLAKVGTRVFLEFTVDHHYQKVAAHVARLPLY
                     NPERKAA"
     gene            7394..9691
                     /locus_tag="VGO32_07000"
     CDS             7394..9691
                     /locus_tag="VGO32_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7200534.1"
                     /translation="MLANRPASTEEAIDALLAEPWLESLTTAHRVIEARPPRHAPWPD
                     DLDPRLVGALRGRGVEALWTHQAQALATVRRRENAVVVTPTASGKTLCYNLPVLDAIA
                     RDDTARALYLFPTKALAADQLVELRALAEAAELDLKTSTYDGDTPPAVRSVVRAAGQV
                     VISNPDMLHAGILPHHTKWFKLFENLQYVVIDELHTYRGLFGSHLANVLRRLQRVCRH
                     YGSDPVFICASATIANPGELAARLIGAPVTVIDDDGAPRGRRHVLIVNPPLVNEALGI
                     RASSLLTAKRIAEQLIGDGVQTIVFGRSRTTVEVMTTYLREALPAAPGHQSSIRGYRG
                     GYLPNERRDIERGLRDGRVRGVVATNALELGIDIGSLDAAISIGYPGTIASTWQQLGR
                     AGRRAGTALSVLVASSAPIDQFLAMHPDWFFDRPPEHGLVNPDNLHLLLDHLRASTFE
                     LPVPADEAFGIVETAALLDVLEEDGFVRRASDDRYHWSHENFPASAFSLRSAAQENVV
                     ILDTTTDPHRVIGEVDLFAAPLLLHEQAIYLHESVQYHVDRLDWDERKAYVRRVDIDY
                     YTDADLGVTLKVLDVADETDAGAGGARARGEVMVAWHVTMFKKIKFHTHENVGWGPVN
                     LPEQQMHTAATWIVLPDAACARFNRETLDGALIGLARLARNTAPLLLMCDARDLGVQA
                     QAQAPFTGKPTLYLYDAVPGGVGLSERIHDLWSDLLAVCRDLVRDCPCLEGCPGCVGP
                     PAEVGIRGKAAATELLETLVAGSRP"
     gene            9688..10920
                     /locus_tag="VGO32_07005"
     CDS             9688..10920
                     /locus_tag="VGO32_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEV7200535.1"
                     /translation="MTQTLAERLAATRSERDTRPRPVRRHDDVAERLAAWGGARVMSS
                     EGGRVALVERRIPLPPPVAARLAGGMRTGYLDTETTGLSTGAGTVVILAAVGHLEDDA
                     IVVRQALLPDYPDEPALLRTVMGWMHGMERMVTYNGRGFDIPLLAARMTIHGLGRDLV
                     GLPERHDDLLPVARRLWRRILGGARLADVERGVLQIRRRSDCPSSEVPQRWFAYQAGA
                     SPDLLAAVIDHNAQDVASLALIDGELRRLRDGGWRDGGLIDHRGLALELLRDGAAEDA
                     LEVLEGVLTGGFGSVPMTGDEPVRLRRLAARLLISGGDSSRAELMWRDAARSGTVEAA
                     LAWIEVARIRERHAADLPGALEATRAASRVLDLALALGRGGSMDRIGRARLQVEGRRR
                     RLTRWVAAAERRAARQVA"
     gene            complement(10922..12160)
                     /locus_tag="VGO32_07010"
     CDS             complement(10922..12160)
                     /locus_tag="VGO32_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV7200536.1"
                     /translation="MTDDIRVLPRPPVDLLVVAGLTIDVFGEVQAVGGAARYATEAAH
                     AAGLRVALHTVAGPEPMMAGALDRLADLSEVSAMPATTSIVFEHHGAHDRRRLRLRRG
                     TEVLDSADAVGLPPASAVLFAPVAAEVSADAVRAIRAPLRAAGLQGWLRQPDADGWVG
                     LVEPERLAADLAETLRDLDLLFASREDLDDMEGPQAVARLRAWAGPGPQLVVTAGVHG
                     AWVDAGDGDPRHVPAEPVEGRNTIGAGDAFAAVLTAGRGSGLDLATAAAEASTATALY
                     LATRTDPTAVAASTDPDRGLADLDGTEWRARRFGAGLEHEPPDSVEFSLEVRGDRVAG
                     RSGCNRFMGGWQLVDDRLRIGPLAGTMMYCDGLMDLEAAYLTALQDVADLARTGDQLI
                     LIGAAGDPVVEYDEVAPVPA"
     gene            12163..12483
                     /locus_tag="VGO32_07015"
     CDS             12163..12483
                     /locus_tag="VGO32_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200537.1"
                     /translation="MSLHYYELHEGATDLLTNALLVSEQAHTGPEFVAMVRAARNAVL
                     ETFEEDTLVEAVARELERTHGFTYIGDEKLDASMSIGATDEDTFLVETSSEYRSIVVE
                     VDRS"
     gene            complement(12496..>13196)
                     /locus_tag="VGO32_07020"
     CDS             complement(12496..>13196)
                     /locus_tag="VGO32_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEV7200538.1"
                     /translation="VVTRRLDKLRTSGRHGTATEREQNAREEELLDRLAPELEEGRPL
                     RELTWSADDEYLARGFRFLSQKPQLVVLNLDEDQLPTATTLEAEAQAAHGGPTRAVIA
                     LGGRIEAEIAALPESEAQPFLDDLGIVEASRGRVIRRTYALLGLQSFLTAGPDECRAW
                     TIPVGATALDAAGVIHSDLARGFIRAEVVDWNDLVACGSMAEARRRGLLRSEGKAYVV
                     RDGDVIEVLFNVGR"
BASE COUNT         2159 a         4616 c         4566 g         1855 t
ORIGIN      
        1 tgggatgctc ttcgcgagcg gatcttcgcg cggcagaagg aaatgggggt catcccggcc
       61 gacgctgagc tgacggcgcg cccagcggaa atcccggcct gggacgacat gcctgacgcc
      121 atgaagcccg tgctggcccg acagatggag gtctacgccg gcttcatgga gcacaccgac
      181 caccatgtgg ggcgattgat cgacgccctg gccgacctcg agatcctgga cgacacgctc
      241 gtctactaca tcgtcggcga caacggtgcg tcggccgaag gcacgccaaa tggcacgttc
      301 aacgagctga tgaccctcaa cggggtgacg accatcgaga cgcccgagtt cctgaccgcg
      361 cgaatcgaca agttcggaac accggaggca tacaaccact acgccgtggg atgggcgcac
      421 gccatggaca cgccgtacca gtggaccaag caagtcgcct cacactgggg cggcacccgc
      481 aatggcacca tcattcactg gccgaatggc ttccaggccc ggggaggtat ccgtcatcag
      541 ttcagccacg tcatcgacat cgcggcaacg gtgctcgatg cggccgggct gcccgaaccg
      601 accttcgtcc acggcgtcca gcagatgcgc ctccatggcc ggagcatgcg cccgaccttt
      661 gacgacgagg cggccccgga gcatcgcgcg acgcagtact tcgagatgtt cgtgaatcgc
      721 gggatatatc accgagggtg gaccgcggtg acccgccaca gcaccccgtg gatcatggcc
      781 gaggcaccgc cgatcgacgc tgatgtatgg gaactctatg ccccggatga ttggacccag
      841 gcccacgacc tggccgctga acagccagag aaactggcct acctccagcg cctgttcctg
      901 atcgaggcga ccaagtacga cgtcctgcca ctcgatgatc ggcgggtcga gcgattcaac
      961 tcggacctgg ccgggcggcc gaccctgatc cacggcaact cgcaggtcct tttcgacggc
     1021 atgggccgtc tgtcagagaa ctcggttctc aacatcaaga acaagtcgca cgcggtcacg
     1081 gcccaggtga cgattcccga cggcgccgcc gccaatggcg tcatcgtggc ccagggaggt
     1141 gcgttcggcg gctgggccgt ctacgcgcat gccgggaagc cggcctactg ctacaacttg
     1201 ttcggattgc agcggttcaa gatctacggc gaccgcgacc tcccagccgg cgaccaccag
     1261 gtccgcgtgg agttcgggta tgacggtggc gggctggcaa agggtggttc ggtcagcttg
     1321 tacctcgacg gcgaaaaggt cggcgaaggc agagtggacc agaccgagcc gatggcgttc
     1381 tccggggatg aaacgaccga tgtcggctcg gatacggcca ccccggtgag cgacgactac
     1441 agctcgcctt cgagcgtctt caatgggcgc atccagtggg tccagatcga cctcggcgac
     1501 gacgcggagg acgccgacca tctcatcacg gccgaggacc gcttccgcgt cgcaatggcc
     1561 cgccagtagg gcgcggcctc aggcacgtcg gtcagggact gtcgcttccg accaggacct
     1621 caatccagcg ctcgagcatc ggcgggtcag gagccgtgac cgggcgggtg ggcatgggtg
     1681 ccgagaccgc cgcgccgggc gggatgatca ggacctgctc gccgggtaat ccgaggttgc
     1741 gcaggcgtcg ggcttcgacg gtcacgtagg cgtcgctctg gaggtactcc agctgccgct
     1801 ggagagccgc gttctcggcc tcggccgccg cgacctgggc ggccagcgag tccgcctgct
     1861 ccccgaccga ccagccaagg aacgcctggc ggccgacgtt gaacgcgatg atggcgacga
     1921 caccggcgac gatgagggcg acgaaggtcc ggcggcgaag gcctacccgg aaagaggggc
     1981 gacgagcagg aggtcgcgcc cgcgtcaccg ctgtgcccgg aacggcggtc atggggcggc
     2041 agtctaggcg gctcgttttg cgggcgtcaa gaaagcgtgc actccatgtc cgctggcaac
     2101 tgctcgggga cggatcgggt accctgattc agccgacggc cccgattgcc cgccaagggc
     2161 cacctcggct ctatttcaca gcccacaggg ccgtcagtgg ccgccgacgc ggccgagagt
     2221 agaagggact aagacagatg ggaatcggac cagtccagta catggtcgtg gcctttccgg
     2281 ggaaccagtt caaaggcgag atcgcgcccg ccctctccga cctggttgag tccggcacga
     2341 tccggatcat cgacctcgcc ttcgtcatca aagaggcgga cgggagcatg accggtgtcg
     2401 agctcgagga cgtgggctca gatgtcttcc aggcgttcga atccctcacc gccgagcgcg
     2461 gcgggttcgt cagcgaggaa gacctccagg ccgtagcggg agccctcgag ccgaactcat
     2521 cggccgcgat cctggtatgg gaggacgttt gggcgaccgg cttcgtggag gccatcgcgc
     2581 gagcggacgg ccagctggtc gacatccagc gtgtgccccg cgagctcgtc gaggaagccg
     2641 tcgcttgggc cgaggaaaac aaggcaacgg ccagcgccta gagcgctgga ccagaaggag
     2701 acagaccgat gagacgacga cggggcggca ttgtccgcat ggccgccacg accgcggtgg
     2761 tcgccggcac agccggtgcc gttagccacc accagcagca gaagtacgcc gcgcaggacc
     2821 agcagcaggc cgcccagcag caggctggat acgaccaggc cgccgcggcc gcgcctccag
     2881 ctcccgccgc cgaggacccg atggtcgaga tccagaagct cgcccagctg cactcggctg
     2941 gcgcgctgac cgacgaggag ttcgccgcgg ccaaggccaa ggtcctcggc atctgaaccc
     3001 gatacctggc gcagccaggc actgaccaaa gaggcccggg ctcctgcccg ggcctctcgc
     3061 gtggcgcagg tggtccgggg tccgaggtgt cggccagggc gcccgggccc cctgctattc
     3121 ggtcctggtt ggtgagccga atacgccgcc acgggagcgg acggctgaga ccggcgtgcc
     3181 ctccatgagg tcgggtgact cggccgacac tcgggcgaac cacgcgccat atcggccggc
     3241 cagtggccag gcgctcatcc cgtgggccac gacgctcagc acgacggtga tcgcgaccgt
     3301 caccgtgatg tccagcgcac ccggcttcgc agccaactcc tcgagcacga tgatggcgaa
     3361 cacgatgctg gccaggcccc gtggcccgaa ccatccgatg aagccaaccg tcgcaggccg
     3421 tgccccgctc ccgacgagag acacggccac tggcagcatg cggatcaccg tcaggctgag
     3481 caacgcgtag accacgtgct cggcccggat ctcggacacg atcggaacaa ggacgccggc
     3541 gccgaacgcg agaaaggtcc ccgccgcgag cagccccccg atcacctcca ggagctcgtt
     3601 cggatgccct tccgcggctg cctcgtcgtg gtcagccctc gccgcccgac gggagatgat
     3661 gccgaatacc gcgccaccga cgaaggcggc gatgaagccg ctgcctccga gcgagaccgc
     3721 caacgcgtac gcagccaccg cggccgtcag gggaatcacc tgggcccatg cctcctgcat
     3781 cgaaccggaa cggcgaccga tccgagccgc cgcggccccc agggctccga ccgcgaatcc
     3841 ggccgcaacc ccgaagccga gctgtcgaac aagcgtggcc agggcatcgc cgacggtcac
     3901 cgcgtcaccc tcggccagcg cgagcgcgac ggcggtaaaa agaatcggga cgcagattcc
     3961 gtcattgaga ccgctttcga cattcagcgc ctggcggata cgggccggca cccgttcgtc
     4021 caggacgact ggtagtccca gggcagcgtc ggtcggcgcc aggcacacgg cgaggacgag
     4081 ggcctcggcc accagcaggc cagggaagag cgggatcgcg acgacggttc ccagggcgat
     4141 ggtcagcacg agcccgatac ccagcagccg ggcggggatc ttccatccag cccgaagctg
     4201 cccgagatga atgctcgagg cgtccgcgaa gagcacgacc gtcagggtca cttcggccag
     4261 cgtgcgcagc ggcacgtcgc ccggctcgat tgggaggacc cccacgaaac ccaggatcgc
     4321 gccgatcccc aggaagtaga tcggggcgct cacgaccgac gccgcgaatc gccgcgagag
     4381 gacggcatac acgaggaacg cgccggccac ggccaggagc caggtcatga cctgagcccc
     4441 gaggcgtccg gtgtggcggc caagggcagc gtggtcacag cgtcaggcgg tgccgcggtc
     4501 cttgaggatc tgccccgcgg ccagcctgcc cggggcgccc atcacgcagc cgcccgggtg
     4561 ggtgcccgag ccgcactggt agtagccgtg gatcggggtc cgatactggg cccagccggg
     4621 tgcgggacga aggaagaaca tctgtggggc gagcagctca ccggcgaaga tgttgccctc
     4681 actcagcccg gtcacccgct cgatgtcgag tggcgtcacg acctcgcgct ggaggaccag
     4741 gtcaccgaac cctgggaagt accgctcgag cgtggcctgg acgacgttcc ccaggttctg
     4801 gcgctcggcg tcccagtcgc tctcccgcag tcggtatggg gtgtactgga cgaagcacga
     4861 catcacgtgc tggccgggcg gggccatgtc cgggtcgatg gtggactgga tggcgccgtc
     4921 gatatacggc tcggcgctgt accagccgta cttcgcctcg tcgaatgcgc gctcgaggta
     4981 gtccatggac ggaccgatgt tcaggaaacc gcggaacggc tcggttccgg catcggcgag
     5041 ctgggggtag accgggatcc cactcagcgc gaagttcacc ttggacgaga caccctggaa
     5101 cttcatccgg cggacggtct cggtcaggtc gtcgggcagc tcacggggct cgaccaggtc
     5161 aaggaacgtg cgccggggat ccagggcgct gaccacgaca tcggcgtgga actcggtgcc
     5221 atcggccagt gcaacgccgg ttgcccggcc gtcatcggtc aggacccggg ccacctctga
     5281 gtcgaggcgg atctcggcgc cgtacgcctg cgcggcgcgg gccaggacct gggtgaaccc
     5341 gccgttgcca cccttgtgga aggcccacgt cccgaacgag ccgtcgtgct cgccgaggga
     5401 gtggaagatg aggacgaggc ccgagccctg cgaacggggc ccgaccttcg agccgatgat
     5461 ccccgacgac gcgagccagc ccttgagcag atccgactcg aagtagtcgt ccaggaagtc
     5521 cgcggcgctg ccggtcagga gccgaatggc gttatggagg gtccgcttgt cgagcttccg
     5581 gaagcctgcg ccaagcgcgg cgagcgccac cagctcttcg gggtcgtcgc tgaacgggtc
     5641 cggcgggacc atgtcaagga gcggcttgag gacctgcgcc acgcggtcca tgtcgtgacc
     5701 gtactgctcg tacgcatcgg cgtcatgcgg ggagatgcga gcgatctcgc gcaggttggc
     5761 gtcgtgatcc tgggtgaacc acaggtactc gccttcgggg gtcggcgcga agcgcgagga
     5821 catgagcagc ggcaggaacc cgtgcttcgg cagctccagc tcctggacga tctgcggacg
     5881 gagcagggac agggcatacg agaaggtcgt gaaccagaac cccgggcgga gctcctcggt
     5941 gatcgcggcc ccacccacga agggccgctg ctcgaggatg agggtcctca ggccaccttt
     6001 cgcgaggtag gcgccattga cgaggccgtt gtggcccccg ccgacgacga tcgcgtcgta
     6061 tctgtccgtc atgcggcctt ccgttcgggg ttgtagaggg gcagccgcgc cacgtgggcc
     6121 gcgaccttct ggtaatgatg gtccaccgtg aactcgagga acacgcgggt tccgaccttc
     6181 gccaggtccg gccgcacccg ggccatggca atgtggcgct gcaggacggg tgagtacatg
     6241 aaactcgtgg cgtagcccac ccgctggctg ccttcgtcgt agaccatcca gtcctcgtga
     6301 actggggcgt gcgccttggg gggaatcagc cccgccgcgc cgtagacccg gtcgtagtcc
     6361 tcccagtcca cgaccagccc gctcattcgc catcgggatg agcggtcggc gatctcgcgc
     6421 tcgagcgcct ttcgaccgat gaaggcgcgg tcgtcatcgg ccagcccctt gaacatccag
     6481 gcccagccca gctcgagcgg ggtggaccgg tgggcgtcgt tgaacgcgaa tcggctggag
     6541 tcgaagtcct tgcccagcag gagcagccca gcctcgatgc ggagcatgta cagcgccgcc
     6601 aacccgtagg cctggacacc gtacccgtcg caggcttccc acagcgtgtc ccacaccgtc
     6661 agcgcgtccg gcgtcgtgca ccagacctcg tatcccaggt caccggtgta gccggtccgg
     6721 gtcacggtga ccggtcggcc accgatctca ccggtcgtga tcccgaagaa cggcacctcg
     6781 gcgacggccg gcacgaggcg ggcgagcagc tcgcgggatc gcggtccctg gaccgccagc
     6841 gcaccgatgt cgaggctgac gtcctcgacg gtgacggcca tgtggccaat ccgatcggcg
     6901 aagtaggcgt agttcggctc ggcggtggtg agcaggtact catccggccc ccgacgctgg
     6961 atgaccgcgt cctcgaacac gaacccgcgg tcgtcgagcc aggtcgtgta ctgggcatgg
     7021 gacgaggggc aggcccggat gtcgcgggcc agcattccgg ccaggaatcg ctcggcgtcg
     7081 cggccggcga tccggtactt gtacagcggc gacgtgtcga agatgcccgc cgcgttgcgg
     7141 accgcgaagt actcgaactt gtccgagacc tggtagcgat tggcggcgag gtggccggcc
     7201 caatggctcc acagccccgt ctcgttcagc gcgctggtcc gctcgtggaa cggcgtggtc
     7261 cgaatcatcg gccgccaacc ctatcaggcg cgacacccaa cctgtcgcac cccgaccgca
     7321 cgctcggaat gacacggagc attagaacag gtgtgctaat ctcccggcca cccatcagcc
     7381 gcttggagcc gacatgctcg ccaatcgacc ggcatcgacc gaggaggcca tcgacgccct
     7441 cctcgcggaa ccatggctcg aatcgctgac caccgcgcac cgagtcatcg aagcgcgacc
     7501 gccgcgccac gccccgtggc cggacgacct cgatccccgc ctcgtcggcg cgctgcgcgg
     7561 ccgtggcgtg gaggcgctgt ggacgcacca ggcgcaggcc ctggccaccg tccgccggcg
     7621 cgagaacgcc gtggtcgtga cgcccaccgc ctcgggcaag accctctgct acaacctgcc
     7681 ggttctcgac gccatcgccc gcgacgacac ggcccgtgcc ctgtatctgt tcccgaccaa
     7741 ggcgctggcg gccgaccagc tggtcgagct gcgggccctg gcggaagcgg ccgagctcga
     7801 tctgaagacg agcacctacg acggcgacac gccaccggcc gttcggagcg tcgtgcgcgc
     7861 cgccgggcag gtggtcatca gcaacccgga catgctccac gccgggatcc ttccgcacca
     7921 cacgaagtgg ttcaagctgt tcgagaacct gcagtacgtc gtcatcgacg agctgcatac
     7981 ctaccgcggc ctcttcggct cgcacctggc caacgtcctc cgtcgactcc agcgcgtctg
     8041 ccgccactac gggtcggacc cggtgttcat ctgcgcctcg gccaccatcg ccaaccccgg
     8101 cgagctggcg gcacggctga tcggcgcacc ggtcacggtc attgacgacg acggtgcgcc
     8161 gcgcggacgg cgacacgtcc tgatcgtgaa cccgccactc gtgaacgagg cactcgggat
     8221 ccgggccagc agcctgctca ccgccaagcg gatcgcggag cagctcatcg gcgatggggt
     8281 ccagaccatc gtcttcggcc gcagccggac cacggtcgag gtcatgacca cctacctccg
     8341 cgaagccctg ccggcggctc cgggccacca gtcctcgatc cgcggctacc gcggtggcta
     8401 tctcccgaac gagcggcgcg acatcgagcg tggcctgcgc gacggccggg tccgcggggt
     8461 ggtcgccacg aacgccctcg agctcgggat cgacatcggt tcgctcgacg cggccatcag
     8521 catcggatac ccgggcacca tcgcttcaac ctggcagcag ctcggccgcg ccggccgacg
     8581 ggccgggacc gcgctgtccg tgctcgtcgc ctcgtccgct cccatcgacc agttcctagc
     8641 catgcatccg gattggttct tcgaccggcc accggagcat ggcctcgtca atcccgacaa
     8701 cctccatctc ctgctcgacc acctccgagc cagcacgttc gagctgccgg tgccggccga
     8761 cgaggcattc ggcatcgtcg agacggcggc cctgctcgac gtcctcgagg aggacggatt
     8821 cgtccgtcgg gcgagcgatg atcggtacca ctggagccac gagaacttcc cggccagtgc
     8881 gttcagcctg cggagcgcgg cgcaggagaa cgtcgtcatc ctggacacca ccacggaccc
     8941 gcaccgggtc atcggcgagg tggacctgtt cgccgccccg ctcctcctcc acgagcaggc
     9001 catctacctc catgagagcg tccagtacca cgtcgatcgc ctggactggg acgagcgcaa
     9061 ggcgtacgtg cggcgggtgg acatcgacta ctacaccgat gcggacctcg gggtgacgct
     9121 caaggttctc gacgtcgcgg acgagaccga tgcgggcgcg ggcggcgccc gcgcccgggg
     9181 cgaggtgatg gtcgcgtggc acgtcaccat gttcaagaag atcaagttcc atacccacga
     9241 gaacgtcggc tgggggccgg tcaacctccc cgagcagcag atgcacaccg ccgccacctg
     9301 gatcgtgctg cccgacgccg cctgtgcccg gttcaaccgg gagacgctgg acggggccct
     9361 catcgggctc gcccggctcg ctcggaacac cgctcccctg ctcctgatgt gtgacgcccg
     9421 cgacctcggc gttcaggccc aggcccaggc gccgttcacc ggcaagccga ctctctacct
     9481 gtacgacgcg gttccgggcg gggtcgggct cagcgagcgg atccacgacc tgtggagcga
     9541 cctcctcgcc gtctgccgag acctcgttcg cgactgcccc tgcctcgagg gctgccccgg
     9601 ctgcgtcggc ccaccggccg aggtcgggat ccggggtaag gcggcggcca ccgagctgct
     9661 cgagaccctg gtggccggct cccggccatg acccagaccc tcgccgagcg gctggccgcc
     9721 acccggtccg agcgagacac ccgaccccgg ccggtccggc gccacgacga cgtcgccgag
     9781 cggctggcgg cctggggcgg tgcccgggtg atgagcagcg agggcggacg tgtggccctc
     9841 gtcgagcggc ggatccccct cccaccgccg gtcgccgccc ggctggccgg cggaatgcgc
     9901 accggctacc tggacaccga gacgacgggg ctgtcgaccg gggcggggac tgtcgtcatc
     9961 ctggccgcgg tcggccacct ggaggacgac gcgatcgtcg tccggcaggc actcctgcct
    10021 gactaccccg acgagccagc cctgctccgg acggtgatgg gctggatgca tgggatggag
    10081 cggatggtga cctacaacgg gcgcggcttc gacatcccgc tgctagcggc gcggatgacg
    10141 atccacggcc tcggtcgcga cctcgtcggc ctgcccgagc gccacgacga cctgctcccc
    10201 gtcgcgcggc ggttgtggcg ccggatcctg ggcggggcgc ggctggccga cgtggagcgg
    10261 ggcgtcctcc agatccgccg gcggagcgac tgcccgtcca gcgaggtccc ccagcggtgg
    10321 ttcgcgtacc aggccggcgc gtcccccgat ctcctcgccg cggtcatcga ccataacgcc
    10381 caggacgtgg catcactcgc cctgatcgac ggggagctgc ggcggctgcg cgatggagga
    10441 tggcgggatg gcggcctcat cgaccaccgg ggcctggccc tcgagctcct gcgggacggc
    10501 gccgccgagg atgcgctcga ggtgctcgag ggggtgctga ccggcggctt cgggtcggtg
    10561 cccatgaccg gcgacgagcc ggtgcgcctc cgccggctgg ctgcgcggct cctcatcagc
    10621 ggcggagact cgtcacgggc cgagctgatg tggcgggacg cggcgcggag cgggaccgtg
    10681 gaggcggcgc tggcctggat cgaggtcgcc cgcatccggg aacgtcatgc cgcggacctg
    10741 ccgggggcgc tggaggccac acgcgccgcg tcgcgcgtcc tcgatctcgc gttggccctt
    10801 ggtcgcggcg ggagcatgga ccgcatcggt cgtgcccggt tgcaggtcga gggtcgccgg
    10861 cgccggctca cgcggtgggt ggccgccgcc gagcggcgag cggcacggca ggtggcctag
    10921 ctcacgccgg gacgggcgca acctcgtcgt attcgacaac cggatcaccg gcggcgccga
    10981 tgaggatcag ctgatccccg gtgcgcgcaa ggtcggccac gtcctggagg gcggtcaggt
    11041 acgccgcctc gaggtccatg aggccgtcgc agtacatcat cgtcccggcc agggggccga
    11101 tccgcaggcg gtcgtcgacc agctgccagc cacccatgaa ccggttgcag ccggaccggc
    11161 cggccacccg atccccgcgc acctcgagcg agaactcgac gctgtccggc ggttcgtgct
    11221 cgaggccggc gccgaaccgg cgcgcacgcc attccgtccc atcaagatcc gccagcccgc
    11281 ggtccgggtc cgttgaggcg gccaccgcgg tcgggtccgt tcgggtggcg aggtacaggg
    11341 cggtcgcggt ggacgcctcg gccgccgccg tggcgaggtc caggccgctc ccccggccgg
    11401 ccgtgagcac ggccgcaaac gcatcaccgg ccccgatcgt gttccgtccc tcaaccggtt
    11461 cggcggggac gtgacgtgga tcgccgtcgc cggcgtcgac ccaggctcca tgcacgccgg
    11521 cggtgacgac cagctgtgga ccggggccgg cccaggcgcg gagccgggcc acggcctgag
    11581 gtccttccat gtcgtccagg tcctcacggc tggcgaacag caggtcgaga tcgcggagcg
    11641 tttccgcaag gtccgcggcg aggcgctccg gctcgacaag gccgacccat ccgtccgcat
    11701 ccggctggcg cagccagccc tggaggccgg ccgcacgtag cggtgcccgg attgcgcgga
    11761 cggcgtcagc ggagacctcg gcggcgaccg gggcaaagag caccgcgctg gcgggaggca
    11821 gaccgaccgc gtccgcgctg tcgagcacct cggtgccgcg ccgcaggcgg aggcggcgcc
    11881 ggtcgtgcgc tccgtgatgc tcgaacacga tggaggtggt tgccggcatg gccgagacct
    11941 cgctcaggtc ggccagccga tcgagcgcgc cggccatcat cggctccgga ccggccacgg
    12001 tgtggagcgc gacgcgcaac cccgcggcgt gcgcggcctc ggtcgcgtag cgggcggcgc
    12061 caccgacggc ctggacctcg ccgaagacat cgatcgtgag gccggcgacc accaacaggt
    12121 caacgggcgg ccggggaagg acccggatat catcggtcac ccatgagcct ccactactac
    12181 gagctgcacg agggcgcgac cgatctcctg accaacgcgc tgctcgtgag cgagcaggcg
    12241 cacaccggcc cggagttcgt ggccatggtt cgcgccgcac ggaacgcggt gctcgagacc
    12301 ttcgaggagg acacgctcgt cgaggccgtg gcgcgcgagc tcgagcggac ccatggcttc
    12361 acctatatcg gcgacgagaa gctggacgcc tcgatgtcga tcggggccac tgacgaggac
    12421 acgttcctgg tcgagacgtc cagcgagtat cgcagcatcg tggtcgaggt cgaccgatcg
    12481 tgaccgcggc cgcgctcagc ggccgacgtt gaagagcacc tcgatcacgt ccccgtcgcg
    12541 tacgacgtag gccttcccct ccgaccgaag gaggccgcgc cgccgggcct cggccatcga
    12601 gccgcaggct accaggtcgt tccagtcgac gacctcggcc cggatgaagc cgcgggccag
    12661 gtccgaatgg atcacccccg ccgcgtcgag cgccgtcgcc ccgaccggga tcgtccacgc
    12721 ccggcactcg tccggccctg cggtcaggaa tgactggagg ccgagcaggg catacgtgcg
    12781 gcggatgacg cggccgcgag aggcctcgac aatgccgagg tcatcgagga acggttgcgc
    12841 ctcggattcg ggcagggccg cgatctcggc ctcgatccgg ccaccgagcg cgatcaccgc
    12901 gcgcgtcggt cctccatgag cggcctgggc ctccgcctcg agggtggtgg ccgtcgggag
    12961 ctggtcctcg tccaggttga ggaccacgag ctgcggcttc tgggacagga accggaatcc
    13021 acgcgccagg tactcgtcat ccgcactcca ggtcagctcg cgcaggggcc gaccctcctc
    13081 gagctcgggc gccaggcgat cgagcagctc ctcctcgcgc gcgttctgct cgcgctcggt
    13141 ggccgtgccg tgccgtcccg aggtccggag cttgtcgagg cggcgggtga ccacgt
//
LOCUS       DASYGU010000247         6001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1120072, whole genome shotgun sequence.
ACCESSION   DASYGU010000247 DASYGU010000000
VERSION     DASYGU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120072"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VGO32_07025"
     CDS             <1..1163
                     /locus_tag="VGO32_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200539.1"
                     /translation="VVGEREAEHQPESMPVAGDEAHARLVQAPRSGARYVLALVTGAV
                     IMILSSPLVDGSFNFLLPVEAYVALFQGAFGSLDRIAETLAEAAPLILAGFAVGIGFK
                     AGLFNIGAQGQFLFGSLFATAAALALNDASPLVAIPASLLAGMVGGLLYGAIPGALKA
                     FTGAHEVVTTIMLNYVAIFLVSGVISGPLRCEDATYSRTCDFTAARLPFILGDSGHLG
                     ILLAFAAVPIVWWFLYRSTTGFEVRTTGANSEAARYAGMSTRWLIIFTMALCGLFAGL
                     AGATWTQGELGYMPASGVPTIGFEAIAVALLGRANPVGILFAGLLFGAMEAGSGAMEL
                     AGIPNEMVTVLQAVILFFLTAEVIVRRIFRVREAAAALGDVGGLAESYGQRL"
     gene            1166..2236
                     /locus_tag="VGO32_07030"
     CDS             1166..2236
                     /locus_tag="VGO32_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200540.1"
                     /translation="MEQLFDIPVIGWLIQFVVYLLGHLPVIAPIIVASAVPIVLGALC
                     GFMNERSGVVNIGIEGMMLTAAFVAWWVASIVYGIIPSSSSSLPFGITIPLLAGLAAA
                     ILAACLVSAVHAWLSITVRADQIISGTIINLFALGITGYLNRLISENPPASAGSFKSI
                     GLPSSITDLPVVGWLIDSFIAVGPIALTMLISVIGLQVLLHRSRWGLRTKAVGEHPKA
                     AETVGIDVIKLRYRNVILGGIFAGLAGAYFSIEFGNEFQANMTNGRGYIALAALIFGR
                     WTPIGAFGGALLFASSVGLERLILFAPPEGELGEFLSGIPIAFYEALPFIITIVILAG
                     VVGRSIPPAADGKPYERESRAA"
     gene            complement(2268..3350)
                     /locus_tag="VGO32_07035"
     CDS             complement(2268..3350)
                     /locus_tag="VGO32_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEV7200541.1"
                     /translation="MTTTLTDEAIMAALARVKDPEIGRDLVALNMVRGIEIEGSLVTV
                     TVELTTPACPLKATIESDIADELTGLGAGAVKIEWAANVKRTFGGPMAELIPGVRNTI
                     AVGSGKGGVGKTTVAVNLAVSLARDGATVGLLDADITGPNVPLMMGTQEGHVTGNQGR
                     VNPVVSHGVKMMSIQYFLTGDAPVIWRGPLIHSAIQQFLRDVEWGELDYLVIDLPPGT
                     GDAALSLAQLIPLSGAVVVTTPQEVSLLDARKAIAMFSKVNVRTLGVVENMSGFVCPH
                     CGTEHDLFGSGGGATIARELGLEVLGRIPLEPSVRAGGDAGVPITSAQHPDSDTSVAA
                     GALREVSRVVAGRISVLAAPVMAAAS"
     gene            complement(3395..4267)
                     /locus_tag="VGO32_07040"
     CDS             complement(3395..4267)
                     /locus_tag="VGO32_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV7200542.1"
                     /translation="MSSIWRPVRERNPSRSLMVIVAHPGDEAFGFAGAIASAAAEGAY
                     VVVVCATRGYFDPRISMKPPAPGGRNRDLLESLGWRNLDTVREDEMRRSAALLGVNVL
                     RMLDYAEGDLPTVDYHHLVGRLVEPIRMHQPEVVLTFGPDGVTGDSDHATLSRAVADA
                     VQQASEPQSYEDDLEEDQVAWRIAKLYHLVVQPEGLEILGGMAPDGIYGTPEAAGPTV
                     DLALGELVALKLAAIERHVSQTGSDGPFAGWDPGRRDAWLADEHYRLAVSNLATAGGS
                     DAGGAETGLFDGLA"
     gene            complement(4286..5362)
                     /locus_tag="VGO32_07045"
     CDS             complement(4286..5362)
                     /locus_tag="VGO32_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7200543.1"
                     /translation="MARPRIDPRTPLSELHVHLGAAVTPAIMWGIAHAQGIRLPTKDY
                     WAFRELITLGRRRRRTFDGYLDLFYWTELIQSSPIAVERSVYEVIGGAYRRSNITRLE
                     LRFNPMKRNRGGEQDLDHIIAAAIRGMDRAILEYPQVQAGLIFCLDRAFSRELNEILA
                     AKAISWRARGVVGVDIAGPVVEGFRFADYRDVIDECRRAGLGITVHAGETGGADEVEE
                     AVEALDPARIGHGIRSATDPRVMAMLRERGIVLEVCPSSNLTTGVVGSIGELRSHLRN
                     LADHEVPFTLSTDGPEILGRYLRDEFALVLGRGILSLEEVERAIETGHQASFVERVPA
                     AERVPAVRHKGRDPSARVALEVEV"
     gene            5440..5808
                     /locus_tag="VGO32_07050"
     CDS             5440..5808
                     /locus_tag="VGO32_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200544.1"
                     /translation="MDLKALIESLASGWAERLTILVPLALVAGLGLYVAYLLIGYLRA
                     SNVGIEAEAVGGEVITPEDAATVALPAGAPYCPVDGLVHPPGAAYCARCEADLARDCQ
                     TCGTRVGAMDAMCFNCGARQ"
BASE COUNT          861 a         2084 c         2049 g         1007 t
ORIGIN      
        1 aggttgtcgg tgagcgggaa gctgagcacc agcccgaatc gatgccggtc gccggggacg
       61 aagcccacgc ccgcctggtg caggcgccgc ggtccggcgc ccgttacgtc ctggccctgg
      121 tgaccggggc ggtgatcatg atcctgtcca gcccgctggt ggacgggtcg ttcaacttcc
      181 tgctgccggt ggaggcgtac gttgcgctgt tccagggtgc gttcgggtcg ctcgaccgca
      241 tcgccgagac cctggccgag gcggccccac tcatcctcgc cggcttcgcg gtgggcattg
      301 gcttcaaggc cggcctgttc aacatcggcg cccagggcca gttcctgttc gggtcgctgt
      361 tcgcgacggc cgccgcgctg gccctgaatg acgcctcgcc gttggtggcc atcccggcct
      421 cgttgctcgc cggcatggtt ggtggactgc tgtacggcgc cattcccggt gcgctgaagg
      481 cgttcaccgg tgcccatgag gtcgtgacca cgatcatgct gaactacgtc gccatattcc
      541 tcgtgtcggg cgtgatctcc ggcccgctgc gctgcgaaga cgcgacgtac tcacggacct
      601 gcgacttcac ggccgccagg ctgccgttca tcctcggcga cagcggtcac ctgggcatcc
      661 tcctcgcctt cgcggcggtg cccatcgtct ggtggttcct gtaccgatca accaccggct
      721 tcgaggtcag gacgacgggg gccaacagcg aggccgcccg ctatgcgggc atgagcacgc
      781 gctggctgat catcttcacc atggccctgt gcggcctgtt cgccgggttg gcgggggcga
      841 cctggactca gggcgagctc ggctacatgc cggcctcggg tgtgccgacg atcgggttcg
      901 aagccatcgc cgtcgcgctg ctcggacggg ccaacccggt cggcatcctg ttcgccggcc
      961 tcctcttcgg cgccatggag gcgggttcgg gagccatgga gctcgccggg atcccgaacg
     1021 agatggtcac cgtcctccag gcagtgattc tgttcttcct cacggcggag gtcatcgtgc
     1081 gccggatctt ccgcgtccgg gaggccgccg cggcgctggg cgacgtgggc gggctggccg
     1141 agtcctacgg gcagcgcctg taggcatgga acagctgttc gacatcccgg ttatcggctg
     1201 gctcatccag ttcgtcgtct acctgctggg ccacctcccg gtcatcgcac cgatcatcgt
     1261 tgcctctgct gtgcccatcg tgctcggcgc actctgcggg ttcatgaacg agcgatccgg
     1321 ggtggtgaac atcggcatcg aggggatgat gctcaccgcc gccttcgtag cctggtgggt
     1381 cgcctcgatc gtctacggga tcatcccttc gtcgtcgagc tcgctgccct tcgggatcac
     1441 gatcccgctc cttgcgggcc tggcggccgc catcctggcc gcatgcctgg tatccgccgt
     1501 gcacgcgtgg ctatcgatca cggtgcgggc cgaccagatc atcagcggca cgatcatcaa
     1561 cctcttcgcc ctcggcatca ctggctacct gaaccggctg atcagcgaga atccaccggc
     1621 cagcgccggc tcgttcaagt ccatcggact gccgtcttcg atcaccgacc tgcctgtcgt
     1681 cgggtggctc atcgattcgt tcattgcagt cgggccgatc gcgctgacga tgctcatctc
     1741 ggtcatcggt ctccaagtcc tccttcaccg gtcccgctgg ggcctccgca cgaaggccgt
     1801 cggggagcac ccgaaggcgg ccgaaacggt cggcatcgac gtcatcaagc tccgctaccg
     1861 gaacgtgatc ctgggcggga tcttcgccgg gctggccggg gcctacttca gcatcgagtt
     1921 cggcaacgag ttccaggcca atatgaccaa tgggcgcggg tacattgccc ttgccgcgct
     1981 catcttcggc cgctggacgc cgattggcgc gttcggcggc gctctcctgt tcgcctcgtc
     2041 cgtcggcctc gagcggctca ttctcttcgc accgccagag ggcgagctgg gcgagttcct
     2101 gagtgggatc ccgatcgcgt tttacgaggc gctgccgttc atcatcacca tcgtcatcct
     2161 ggccggggtc gtcgggcgaa gcattccgcc ggcagcggac ggaaagccat acgaacggga
     2221 gtcgagagcg gcctgatctc gcgccgcccg gttggggcgg gtgtgaccta gctggcggcg
     2281 gccatcaccg gcgcggcgag gaccgagatc cgaccggcga ccacgcgcga cacctcgcgc
     2341 agggcaccgg ccgcgacgga cgtgtccgaa tcggggtgct gggcggaggt gatcgggaca
     2401 cccgcgtcgc caccggcgcg cacgcttggc tcgagcggga tgcggccgag aacctccagg
     2461 cccagctcgc gggcgatggt cgcgcctccg ccggagccga acaggtcgtg ctcggtgccg
     2521 cagtgggggc atacgaagcc gctcatgttc tccaccactc ccagggtccg gacattgacc
     2581 ttggagaaca tcgcgatcgc cttccgcgcg tcgaggagag agacctcctg gggcgtggtc
     2641 acgaccaccg cgcccgacag cgggatgagc tgggcgagcg agagcgccgc gtccccggtt
     2701 ccgggcggca ggtcgatgac gaggtaatcc agctcgcccc actcgacgtc gcgcaggaac
     2761 tgctggatcg ccgagtggat gagcggtccg cgccagatca ccggcgcgtc gccggtcagg
     2821 aagtactgga tgctcatcat cttcacgccg tggctgacca ccgggttgac ccgaccctgg
     2881 ttgccggtga cgtggccttc ctgggtgccc atcatgagcg ggacgttggg accggtgatg
     2941 tccgcgtcca gcaggccgac cgtggccccg tcgcgggcca ggctgaccgc gagattgacc
     3001 gcgaccgtgg tcttgcccac cccacccttg ccggacccca ccgcgatcgt gttccgcact
     3061 ccggggatga gctcggccat aggcccgccg aacgtgcgct tcacgttcgc ggcccactcg
     3121 atcttgactg cgccagcgcc caggccggtc agctcgtccg cgatgtccga ctcgatggtt
     3181 gccttcagcg ggcaggccgg ggtggtgagc tcgaccgtga ccgtgaccag cgatccctcg
     3241 atttcgattc cgcgaaccat gttcagggca acgaggtcgc gcccgatctc cggatccttg
     3301 acccgggcca gggcagccat gatcgcctcg tcggtcagcg tggtggtcac cggaccgagc
     3361 ctatcagacg gtggtgatcg ccgccgaact acgctcaggc caggccgtca aacagccccg
     3421 tctcggctcc accggcgtca gatccacccg cggtggccag gttcgagacc gcgagccgat
     3481 agtgctcgtc tgccagccag gcatctcgcc ggccagggtc ccagcccgcg aacgggccgt
     3541 cggagccggt ctgcgacacg tggcgctcga tggccgccag cttgagcgcg acgagctcgc
     3601 cgagggcgag atcgaccgtg ggtccggcgg cctcgggcgt gccgtagatc ccatccgggg
     3661 ccatcccgcc caggatctcg agtccctcgg gctggacgac gaggtggtac agcttggcaa
     3721 tccgccacgc cacctggtcc tcctcgaggt cgtcctcgta ggactggggc tcggacgcct
     3781 gttggaccgc gtcggccacc gcgcgggaca gggtggcgtg gtcgctgtcg ccggtgaccc
     3841 cgtccggacc gaaggtcagg acgacctcgg gctggtgcat ccggatcggc tccaccaggc
     3901 gtccgacgag atggtgatag tccaccgtcg gcagatcgcc ctcggcgtag tcgagcatgc
     3961 ggagcacgtt caccccgagc agtgcggcgg agcggcgcat ctcgtcctcg cggacggtgt
     4021 ccaggttgcg ccagcccagc gactcgagca gatcgcgatt gcggccgccc ggggcgggtg
     4081 gcttcatcga aatccggggg tcgaagtagc cccgggtggc gcacaccacg accacgtatg
     4141 caccctcggc agcggcgctc gcaatggccc cggcgaaccc gaatgcctcg tcgcccggat
     4201 gggcgacgat caccatcagg ctgcgggacg ggttccgttc gcggaccggc cgccagatcg
     4261 acgacacggc gccgagtcta caggttcaga cctcgacttc gagggcaacc cgggccgacg
     4321 gatcgcggcc cttgtgccgc accgcgggca cccgttcggc ggccggaaca cgctcgacga
     4381 agctggcctg atggccggtc tcgatcgccc gctcgacttc ctccaggctg agaattccgc
     4441 gacccaggac gagggcgaac tcgtcccgca ggtaccgacc caggatctcg ggaccgtcgg
     4501 tcgagagcgt gaacgggacc tcgtggtcgg ccaggttgcg aaggtgcgaa cgcagctcgc
     4561 cgatgctgcc caccaccccg gtggtcaggt tggacgaggg gcagacctcg agcacgatcc
     4621 cccgctcgcg gagcatggcc atcacccgcg ggtcggtcgc cgagcggatg ccgtgaccga
     4681 tgcgcgccgg gtcgagcgcc tccactgcct cctcgacctc gtccgctccc ccggtctcgc
     4741 cggcatggac ggtgatcccc agcccggcgc ggcggcactc gtcgatcacg tcgcggtagt
     4801 ccgcgaaccg gaatccctcg accacgggac cggcgatgtc gactcccacc acgccgcgcg
     4861 cccgccagct gatggccttg gcggcaagga tctcgttcag ctcgcggctg aaggcgcggt
     4921 ccaggcagaa gatgagcccg gcctggacct gcgggtactc gaggatcgcg cgatccatgc
     4981 cccgaatcgc ggccgcgatg atgtggtcca ggtcctgctc accgccccgg ttgcgcttca
     5041 tcgggttgaa gcgcagctcc aggcgcgtga tgttgctgcg ccgataggcc ccgccgatga
     5101 cctcgtacac gctgcgctcg accgcgatcg ggctcgattg gatcagctcg gtccagtaga
     5161 agaggtcgag atagccgtcg aacgtgcggc gccggcgtcg gccgagggtg atcagctcgc
     5221 ggaaggccca gtagtccttc gtcgggaggc ggatgccctg cgcgtgggca atcccccaca
     5281 tgatggccgg ggtgacggcc gctccgaggt gcacgtggag ctcgctgagc ggcgtgcggg
     5341 ggtcgatccg gggtcgggcc aaactgccac cggctacggg ctttggcgga gtgtagcagc
     5401 ggtccggcga cgggacaccc gcgctagaat cgatgtccga tggacctcaa ggcgctgatc
     5461 gaatcactcg cctccggctg ggccgaacga ctcaccattc tcgtgccgct ggccctcgtc
     5521 gccggactcg gcctgtacgt ggcctacctc ctgatcggct acctccgcgc ctcgaacgtg
     5581 gggatcgagg ccgaagcggt gggcggcgag gtcatcacgc cggaggatgc ggcgaccgtc
     5641 gcactccccg ccggggcgcc ctactgcccg gtggacgggc tcgtccatcc gcccggtgcc
     5701 gcctactgcg cccggtgcga ggcggacctc gctcgtgact gccagacgtg cgggaccagg
     5761 gtcggggcga tggacgccat gtgcttcaac tgcggggcgc gccagtagtc cacccggctc
     5821 gtcggccgac cgctcagttc cccatcagcg actgtacgta ggctcgcatc tcgtacacgt
     5881 tcgcgacctg cacgtagccg cggccggtgc cgggctccag accggtgaac agcgagaagc
     5941 gcggcggggc gaggaccgtg gcggtcacgt tcgcattggc ggcctcgccg accacgttga
     6001 g
//
LOCUS       DASYGU010000248         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1171895, whole genome shotgun sequence.
ACCESSION   DASYGU010000248 DASYGU010000000
VERSION     DASYGU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171895"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VGO32_07055"
     CDS             <1..578
                     /locus_tag="VGO32_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEV7200545.1"
                     /translation="DLVLADDIGQPPTRVDIGALRVHVIRRGERIGLRVRDTEAAVLE
                     SFTGVPRFAVDPAWRVTGHLERAPGLRTVDVPDVLGEVRAEESSGVVHFELGGEHLHL
                     DALPEDDDMLWLIFGDTTNGTETYSGGRFLVTGPVAEDGSVVVDFNLAYNPPCVFSPY
                     ATCPLPWPENRLPIAVEAGERLPHVPGRPAD"
     gene            591..1790
                     /locus_tag="VGO32_07060"
     CDS             591..1790
                     /locus_tag="VGO32_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7200546.1"
                     /translation="MTTDATGTVRAPAEFLNDTVRAIQPSGIRRFFDMLAEMKDVISL
                     TIGEPDFTTPEPLTRAAIAALEAGETHYSANGGMIELRELVAANLADRYGVRYEPRGE
                     LIITVGASEGLDATLRAIINDGDEVIYHEPCFVAYAPLITLGGGVPVAIATSDQADFR
                     VTAAQIEAAVTPRTKAIFLGYPNNPTGAVLDRDELTAIGEVAERYDLLVISDEIYDRL
                     VYGEHRHTAISALPGMRERTVLIGGFSKAYAMTGWRIGYVAAPAGLMEGIAKVHQYGI
                     MCAPTAAQHAAIAALQVGEPFVQDMLAEYDRRRQLMTRRFNEIGLTCFEPMGAFYCFP
                     EVTAATGLSAEDFAQQLLAEEHVAVVPGEAFGPSGAGHVRACYATAYEQIVEAMDRIE
                     AFVARHR"
     gene            complement(1829..2557)
                     /locus_tag="VGO32_07065"
     CDS             complement(1829..2557)
                     /locus_tag="VGO32_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200547.1"
                     /translation="MRAAALLLLGSLMLAACQPSASTSPSPSVAASEAPSEPAPSETA
                     AAVCDPGVICNGPLRAGDYFSATTGARVEFTLDEHDWFGQEDTPGDGFSLFLADVDEG
                     AISVVSFNGEIFTNSCDPEAGTLQLEDSPAAFMSMLTSRSGITATTPVEVEVGGQPAL
                     QTDLTVALDADCTATGNGRAYLWTLPVHGDFHFDDTEQARVIAVHTDSATVILVVEAF
                     PSVDWDHLLEHFTEVIETMTISPL"
     gene            complement(2585..3004)
                     /locus_tag="VGO32_07070"
     CDS             complement(2585..3004)
                     /locus_tag="VGO32_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200548.1"
                     /translation="MRIGEWENIGRSRLLVQRELGELGANEMATHVSIHADGERQRIL
                     IATDIGLLDYNFQPSSPGGAVYELRGTLYRWPSIRGMRLQSDAQWDDNAKTAQSLWRL
                     IAEEPRIELAAQSTETDRHDVSALLDFARAAFASLDK"
     gene            3028..>4220
                     /gene="gatB"
                     /locus_tag="VGO32_07075"
     CDS             3028..>4220
                     /gene="gatB"
                     /locus_tag="VGO32_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV7200549.1"
                     /translation="MATASVLPTYETVIGIEVHAQLRTSSKMFCGCPTAPDPGEEDAP
                     NTRVCPVCMGLPGTLPVINRRAVELVMLTGLALEAEVQTEAVRFERKNYFYPDLPKGY
                     QISQYALPLTANGRLLVPVAAEEGEITVGITRAHLEEDTARLLHGGRDHSLVDFNRAG
                     VPLMEVVTEPDLRSPAQARAYGETLRDLLRFIGASDADMEAGSMRIEGNVSIREVGSD
                     VFGTKVEVKNLNSFRSLERAMEFEVERMAESLAAGVPLTQETRGWDETGGKTIPQRSK
                     EEANDYRYFPEPDLPPLRPSPEWVAEIRSGLPELPAARRARYVAELGLSPYDAGVLSA
                     EVSLGDYFDEVVAAGVPAKAAANWVSGEFSRLLNQHAAEGLRAAGVALRPDGLAEIIQ
                     EVEAGR"
BASE COUNT          655 a         1463 c         1469 g          633 t
ORIGIN      
        1 gcgacctggt cctggccgat gacatcggcc agccgccgac ccgggtggac atcggtgcgc
       61 tccgcgtcca cgtcatccgc cgcggcgagc gcatcggtct gcgggtccgt gataccgaag
      121 ccgccgtcct cgagtccttc accggcgtcc cgcgcttcgc ggtcgatccc gcctggcggg
      181 tgaccggcca tctcgagcgg gcgccgggcc tccggacggt ggacgtgccc gatgtcctcg
      241 gcgaggtgcg ggccgaggag tcgtccgggg tcgtccactt cgagctgggc ggggagcacc
      301 tccacctcga cgcgctgccg gaggacgacg acatgctgtg gctgatcttc ggcgacacca
      361 cgaacgggac ggaaacctac tccggcggcc gcttcctcgt gaccggcccc gtggcggagg
      421 acggatcggt cgtggtggac ttcaacctcg cctacaaccc gccgtgcgtg ttcagcccgt
      481 atgcgacatg tccgctgccg tggccagaga atcggctgcc gatcgccgtg gaggccgggg
      541 agcggctgcc ccacgttccg ggccggccag cggactagac ttccagaccg atgaccaccg
      601 acgccaccgg caccgtccgg gctcccgccg agttcctgaa cgacaccgtt cgggccatcc
      661 agccctcggg cattcgccgc ttcttcgaca tgctggccga gatgaaggac gtcatcagcc
      721 tgaccatcgg cgagccggac ttcacgaccc ccgagccgct gacccgggcg gcgatcgccg
      781 ccctcgaggc gggggagacg cactactcgg ccaacggcgg gatgatcgag ctccgcgagt
      841 tggtggccgc caacctggcc gatcggtacg gcgtccgcta tgagccccgc ggggagctga
      901 tcatcacggt cggcgcgtcg gaggggctgg acgccaccct gcgggcgatc atcaacgacg
      961 gtgacgaggt catctaccac gagccttgct tcgtggccta cgccccgctc atcaccctgg
     1021 gcggcggcgt cccggtggcc attgccacgt cagaccaggc cgacttccgg gtcaccgccg
     1081 cccagatcga ggcggccgtc acgccccgta ccaaggccat cttcctgggc tacccgaaca
     1141 acccgaccgg cgcggtcctc gaccgcgacg agctgacggc catcggcgag gtggccgagc
     1201 ggtacgacct gctggtcatc agcgacgaga tctacgaccg gctggtgtac ggggagcatc
     1261 ggcataccgc gatcagcgcc ctgcccggga tgcgggagcg aaccgtgctc atcggcggct
     1321 tttcgaaggc gtatgcgatg accggctggc ggattggata cgtggctgcg ccggccggcc
     1381 tgatggaggg gatcgccaag gtccaccagt acgggatcat gtgtgccccc accgccgccc
     1441 agcacgcggc catcgccgcg ctccaggtcg gagagccgtt cgtccaggac atgctggccg
     1501 agtacgaccg ccggcggcag ctcatgaccc ggcgcttcaa cgagatcggc ctgacctgct
     1561 tcgagccgat gggcgcgttc tactgcttcc ccgaggtgac cgccgcgacc ggcctgtccg
     1621 ccgaggactt tgcccagcag ctcctggccg aggagcacgt cgcggtggtt cccggcgagg
     1681 ccttcgggcc ctctggcgcg ggccatgtcc gggcctgcta cgcgaccgcc tacgagcaga
     1741 tcgttgaggc catggaccgc atcgaggcct tcgtcgcccg ccaccgctga tcgggatggc
     1801 cggtgcctgg gcgcaccggc ggtggaacct agagcggcga aatcgtcatc gtctcgatta
     1861 cttcggtgaa gtgctcgagc aggtggtccc aatcgacgct ggggaaggcc tcgacgacca
     1921 ggatcacggt cgcgctatcc gtgtggacgg cgatgacccg cgcctgctcg gtgtcgtcga
     1981 agtggaagtc accgtggacc ggcagggtcc aaaggtacgc gcggccgttg ccggtcgccg
     2041 tgcagtccgc gtccagggcc accgtcagat cggtctgcaa cgcgggctga ccgccgacct
     2101 ccacctcgac cggggtggtg gcggtgatcc cgctccgtga ggtgagcatg ctcatgaacg
     2161 cggccggcga gtcctccagc tggagcgtgc cggcctcggg gtcgcaggag ttcgtgaaga
     2221 tctcgccgtt gaacgagacg acgctgatcg cgccctcatc gacatcggcc aggaacaggc
     2281 tgaacccgtc acccggcgtg tcctcctggc cgaaccagtc gtgctcgtcg agggtgaact
     2341 cgacccgcgc cccggtcgtg gccgagaaat agtcgccagc tcggagcgga ccgttacaga
     2401 tgacccccgg atcgcatacg gcggcagcgg tctccgatgg cgccggctcg gacggcgcct
     2461 cggacgcggc gaccgagggc gagggagacg tcgaagccga tggctggcac gccgccagca
     2521 tgagcgaccc aagcaacagc aaggcagccg ctctcatcgg cccggccgca tcggtacgac
     2581 ggctctactt gtccagggag gcgaatgccg cgcgggcgaa gtccaggaga gcggagacgt
     2641 catgccggtc ggtctccgtg gactgggcgg caagctcgat ccggggttcc tcggcgatga
     2701 gccgccatag cgactgagcg gtcttcgcgt tgtcgtccca ttgggcgtcg ctctggaggc
     2761 gcatgccgcg gatcgagggc cagcggtaga gcgtgccccg cagctcgtag accgcgccgc
     2821 caggcgacga gggctggaag ttgtagtcca gcaggccgat atcggtcgca atcaggatcc
     2881 gctgccgctc gccgtcggcg tggatcgaga cgtgggtcgc catctcgttc gcgcccagct
     2941 cacccagctc acgctggacg aggagccgcg accgaccgat attctcccat tcgccgatgc
     3001 gcacgggccg gagcatacaa tccggccgtg gccaccgctt ccgtacttcc gacctacgag
     3061 accgtgatcg ggatcgaggt ccacgctcag ctgcgcacgt cctcgaagat gttctgtggc
     3121 tgtcccaccg cccccgaccc gggcgaggag gacgccccga acacccgggt ctgccccgtg
     3181 tgcatgggtt tgcctggcac gctgccggtc atcaaccgcc gcgcagtcga gctcgtgatg
     3241 ctcaccggcc tggccctgga agccgaggtc cagaccgaag cggtccggtt cgagcgcaag
     3301 aactacttct accccgatct gcccaagggc taccagatca gccaatacgc gctgccgctg
     3361 accgccaacg gccggctgct ggtccccgtc gcggccgagg agggggagat caccgtcggg
     3421 atcacccgtg cccacctgga ggaggacacc gcgcggctgc ttcacggcgg gcgggaccac
     3481 tccctggtgg acttcaaccg ggcaggcgtg cccctgatgg aggttgtgac cgagccggac
     3541 ctccgctcac cggctcaggc acgcgcctac ggggagacgc tccgtgacct gctccgcttc
     3601 atcggcgcgt ccgatgcgga catggaggca ggatccatgc ggatcgaggg caacgtgagc
     3661 atccgtgagg tcgggagcga cgtgttcggc accaaagtcg aggtcaagaa cctgaactcg
     3721 ttccgctcgc tggagcgggc gatggagttc gaggtcgagc ggatggcgga gtcgctcgcc
     3781 gccggcgtgc cgctgaccca ggagacccgc ggctgggacg agaccggggg caagacgatc
     3841 ccccagcgct cgaaggaaga ggccaacgac taccgctact tccccgagcc cgacctgccg
     3901 ccgcttcgcc cgtcgcccga atgggtggcg gagatccgct cggggctgcc tgagctgccc
     3961 gccgcgcggc gtgcccgcta cgtggccgag ctgggcctct ccccgtacga cgcgggcgtc
     4021 ctgagcgcgg aggtcagcct gggggactac ttcgacgagg tggtggccgc cggcgtcccg
     4081 gcgaaggcgg cggccaactg ggtgagtggc gagttcagcc ggctcctgaa ccagcacgcc
     4141 gcggagggcc ttcgcgcagc gggcgtcgcg ctccgcccgg atgggctggc cgagatcatc
     4201 caggaggttg aggccggacg
//
LOCUS       DASYGU010000249         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_40614, whole genome shotgun sequence.
ACCESSION   DASYGU010000249 DASYGU010000000
VERSION     DASYGU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40614"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VGO32_07080"
     CDS             <1..1077
                     /locus_tag="VGO32_07080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV7200550.1"
                     /translation="HLRALIYSRSGDLEAPIVTVSVDDEGQPIGSGGCEASDWASFPE
                     GSIALAGPGPCFRRQMVEEAQNAGAVALIVSSASYEPGAVRRPTLIDPDGIEIPALYA
                     SNEAGAALLVAAQAGGSVHLSVETLIQDRITANVLAERTGSQGDPETAPVVMMGGHLD
                     SVIDGPGINDNGSGTGTILEIALQLAEMEPTDRTVRFAFWAAEELGLMGSYQWVTSQD
                     QEELDRIVAYLNLDMVGSPNFVRQVYDSSSAVTASGEITDAFGAYFDAVDLTWESEDI
                     GGGSDHAGFEDFLIPTGGLFSGASEEKSAAQANLFGGQADEPIDPCYHLTCDVADQVN
                     DEVLDQLSDAAAHVLMLLLTGRLL"
     gene            complement(1074..>1743)
                     /locus_tag="VGO32_07085"
     CDS             complement(1074..>1743)
                     /locus_tag="VGO32_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7200551.1"
                     /translation="ILAWPLHPLTRVVGLTFQELVTSPILNAFYRIGIRGQENLVGLE
                     GPVLFASNHCLHTDNQIVMSSMPFVWRWRLSVAASAERVWSKPAMAVAGGLLANAFPI
                     AREGGARRSFELLGARLDRRFSILIYPEGELTVGGPMKPFLAGTGLVAVESATAVVPT
                     KLLIHRMSWLDAHWPGSENGNHNGTSSLRGEVEVVFGEPIWFASDTTPADATERLEQA
                     MAAL"
BASE COUNT          279 a          586 c          620 g          258 t
ORIGIN      
        1 cacctccggg cgctcatcta ctcacgttcc ggcgacctcg aggcgccgat cgtgacggtg
       61 agcgtcgacg atgaaggcca accgatcggg tccggcggat gcgaggcgtc ggactgggcc
      121 agcttccccg agggctcgat cgccctggcc ggccccggtc catgcttccg gcggcagatg
      181 gtggaggagg cccagaacgc cggcgccgtg gcgctgatcg tgtcctcggc gagctacgaa
      241 ccgggcgccg tgcggcggcc gaccctgatc gatcccgatg ggatcgagat cccggccctg
      301 tacgcctcga acgaggccgg ggcagccctg ctcgtcgctg cgcaggccgg gggatcggtc
      361 catctgtcgg tcgagaccct catccaggat cggatcacgg ccaacgtcct cgccgagcgg
      421 accggcagcc agggcgaccc cgagacggcg cccgtggtga tgatgggggg gcacctggac
      481 tcggtcatcg acggaccggg aatcaatgac aacgggtcgg gcaccggcac catcctggag
      541 atcgccctcc aactggccga gatggagccc accgaccgga cggtccgctt cgcgttctgg
      601 gcggccgaag agctcggcct gatgggctcc tatcagtggg tcaccagcca ggatcaggag
      661 gagctcgacc ggatcgtggc gtacctcaac ctggacatgg tcgggtcgcc gaacttcgtg
      721 cgccaggtgt acgactcctc ctccgcggtc accgcctcgg gcgagatcac cgacgcgttc
      781 ggcgcgtact ttgacgcggt ggatctgacc tgggagagcg aggacatcgg cggcggatcg
      841 gaccatgccg gtttcgagga cttcctgatc ccaactggcg gactgttcag tggggcgtcc
      901 gaggagaaga gcgcggcgca ggcgaacctg ttcggcgggc aggcagacga gccgatcgat
      961 ccctgctacc acctgacctg tgacgtcgcc gaccaggtca acgacgaggt gttggaccag
     1021 ctctcggacg cggccgccca cgtcctgatg ctgctgctga cgggccgcct gctctagagg
     1081 gcggccatcg cctgctcgag gcgctcggtg gcgtcggccg gggtcgtgtc ggacgcgaac
     1141 cagatcggct ccccgaacac gacctccacc tccccgcgca gcgaggacgt gccgttgtgg
     1201 ttcccgttct cgctgccggg ccagtgggca tcgagccacg acatgcggtg gatcaggagc
     1261 ttggtgggca ccaccgccgt cgccgattcg actgcgacca ggccggtgcc ggcgaggaac
     1321 ggcttcatcg gtccgcccac cgtcagctcg ccctcggggt agatgaggat gctgaaccgc
     1381 cggtccagcc gggcgcccag cagctcgaag cttcgccgcg cgccgccctc gcgggcgatc
     1441 gggaaggcat tggccagcag gccgccggcg acggccatgg ccggcttgct ccagacgcgc
     1501 tccgccgatg cggcgaccga cagccgccag cgccagacga acggcatgga cgacatcacg
     1561 atctggttgt cggtgtggag gcaatggttc gaagcgaaca ggaccgggcc ttccagcccg
     1621 accaggttct cctggccgcg gatcccgatc cggtagaacg cgttgaggat gggggaggtg
     1681 accagctcct ggaaggtcag gccgaccacc cgggtcagcg gatggagcgg ccaggccagg
     1741 atg
//
LOCUS       DASYGU010000250         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_812658, whole genome shotgun sequence.
ACCESSION   DASYGU010000250 DASYGU010000000
VERSION     DASYGU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812658"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VGO32_07090"
     CDS             <1..1407
                     /locus_tag="VGO32_07090"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7200552.1"
                     /translation="LVYVYVLGNGLFALLARPFAPLPDRLASAASRLRGVPAVLDAAR
                     ANLLDPGDRPVARFHTEKAIERLPGIPGLVDAVLAEVDKVEDADAAAALRAELEPAAA
                     AAREALATFGTWLSDELLPTATGDFRLGPALYRAKFRHALRVEMEPTELRARALGAFD
                     VVRAEMQRLAIELWPTWKGDDPMPDQPDVVVRGVLDAIATDHSAAGELLDFCRAENDR
                     IEAFCRDIGLIGLADEPLEIVWTPEFLRSFGGAMLIPPGPLDRGLSSFFAITPMPDDW
                     TDAQRESYLREDNARMLRLLTIHEAVPGHYLQLAYSNRCPSLVRAIFQSGVFAEGWAV
                     YVTQVMMDVGYGAEDPALMLVHWKFYLRAITNTLMDIAIHTGAMEREEAMKLMVEQGF
                     QEESEASAKWDRARLSSTQLCEYFLGYTEMADLEREARARAAEAGTPFRWRHHLEAVL
                     AHGTPPTPVIRDILFGDT"
     gene            1412..2650
                     /locus_tag="VGO32_07095"
     CDS             1412..2650
                     /locus_tag="VGO32_07095"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7200553.1"
                     /translation="MGRGFGALRHRNFRLYWSGQVVSLVGTWMQSVSQPWLVLELGGS
                     ALQVGTVIALQFAPALFLAPIGGVLADRFDKRGLLLVTQALAMLQAATLFGLTFSGGI
                     EIWQIQVLALILGITSAFDMPVRQSFVAEVVPRRDLLQAIALNSASFNLARVVGPAVA
                     GITLALFGSAFNFGINTLTYTAVLVGLLLIDPKTLIRVPIPAIQPSIRTSLREGFQYA
                     AQTPSVLWLLVLLGGMSVFAMNFQTLLPLFARFTLDMDAGAYGALFAVMGAGSLVGSL
                     VLAFMGESRPRLRLILGGGVAFLVFEVMLGLIRTPALAYPVVALVGLASMLMVNTINA
                     TVQRSVPDELRGRVMALYVTVFAGSGPLGGLFAGAVAQVLGPPAGFIIGAALASIFLA
                     LTWWKLGRRGPMPLTAPTPA"
     gene            2679..3920
                     /locus_tag="VGO32_07100"
     CDS             2679..3920
                     /locus_tag="VGO32_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7200554.1"
                     /translation="MTAPATAAESNRANLTLGLLALAHSLIHMQSAVMPLVYIAVIDQ
                     FGLNEASIGLFIAVTTAVAGAMQLSYGFLTRYIARPVLIGVGQLVFGVSLMLAGFATT
                     IGQLLATISGARVGASPQHPVGNGILSDAFPPERRGFAISTHIAGGNVGTILVPFIGG
                     ALLASVGWNLTLVAFGIPALLVGVLILWFVREDSRTYRRDARAAGSWSSHVRAVFRRR
                     DLMKILAAAMVAAGGRGLDIAAPFLLLYFRSELGIDDNTVLWLYALLLVGAVVGPVLA
                     GILSDRYGRRRTLIVYYVLSSVGILSVVLVGGNLLLLAPLLLPFGTAVFSEGPVLQAF
                     LADRAVGPIRDVAFSIYFTAAFGIGALWALIIGAIVAGPGYVTAFVVMAVSYIAGAIL
                     ISLVRDRPPDVGLDAAGTEPA"
     gene            complement(3917..4348)
                     /locus_tag="VGO32_07105"
     CDS             complement(3917..4348)
                     /locus_tag="VGO32_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200555.1"
                     /translation="MRDGLRLPWPLGLIWALPVTLFGVAILVAGALTGGHVAFVSGAF
                     EGYGGVIRRGLKVVPVGHGGAGALTLGHVVLGANARALEVSRRHERVHVAQYERWGIV
                     FPVAYVASSLSALLHGQNAYRGNRFEKEANRLSRRKTGQPA"
     gene            4426..>5136
                     /locus_tag="VGO32_07110"
     CDS             4426..>5136
                     /locus_tag="VGO32_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7200556.1"
                     /translation="MTSDSATIENLLREDRTFPPPPAFAAAAIANDPDIYERTATDEG
                     FEAFWAEESRRIDWIEPWQRLLKWEAPFAEWFVGGKLNVSVNCLDRHVANGLGDKVAY
                     HWEGEPGDHRTITYAQLLAETCQMANALRELGVKKGNRVAIYMPMIPELPVAMLACAR
                     IGAPHSVVFGGFSADALAGRIDDAQCTTVITADGGYRKGALVPLKDNADDAMGRSPSV
                     EHCIVVRRTGQDVAWQEGR"
BASE COUNT          690 a         1872 c         1724 g          850 t
ORIGIN      
        1 ctggtctacg tgtatgtcct gggcaacggc ctgttcgcgc tgctcgcgcg accgttcgcc
       61 ccgcttcctg atcggctggc cagcgccgcc tcgcggctgc gcggcgtgcc ggccgttctc
      121 gacgcggctc gtgcgaacct cctggacccc ggcgaccgcc cggtggcccg gttccacacc
      181 gagaaggcca tcgaacggct gcccgggatc cccgggttgg tggacgccgt cctggccgag
      241 gtggacaagg tcgaggacgc ggacgccgca gcggccctgc gggccgagct cgagccggca
      301 gccgcggccg cacgggaggc gctggcgacg ttcggaacct ggctgagcga cgagctcctg
      361 cctacggcaa ccggcgattt ccgactcggg ccggcgctgt accgggccaa gttccggcat
      421 gcgctccggg tcgagatgga gccgaccgag ctccgggcac gggcgctggg cgcgttcgac
      481 gtcgtgcgcg ccgagatgca gcgactggcc atcgagctgt ggcccacgtg gaagggcgac
      541 gatccaatgc cggaccagcc ggacgtcgtg gtccgcggcg tcctggacgc gatcgcgacc
      601 gaccactcgg cggccggcga gctgctggac ttctgccgcg cggagaacga tcggatcgag
      661 gccttctgcc gcgatatcgg cctcatcggc ctggccgacg agccgctcga gatcgtgtgg
      721 acgccggagt tcctccggtc gttcggcggg gccatgctca ttccgcccgg cccgctggac
      781 cgcggcctgt cgagcttctt cgcgatcacg cccatgcccg acgactggac cgatgcccag
      841 cgcgagtcct acctccgcga ggacaacgcc cgcatgctcc ggctgctgac catccacgag
      901 gcggtgccgg gccactacct ccaactggcg tactcgaacc gctgcccatc cctggtgcgc
      961 gcgatcttcc agtccggcgt cttcgccgag ggttgggcgg tgtacgtgac ccaggtgatg
     1021 atggacgtcg gctacggcgc cgaggatccg gcgctcatgc tcgtccactg gaagttctac
     1081 ctgcgggcca tcaccaacac cttgatggac atcgcgatcc acaccggggc catggagcgc
     1141 gaggaggcca tgaagctcat ggtcgagcag ggattccagg aggaatccga ggcctcagcg
     1201 aagtgggacc gggcccggct gtcgagcacc cagctgtgcg agtacttcct gggctacacc
     1261 gagatggccg atctcgagcg cgaggcccgc gcccgagcgg ccgaggccgg gaccccgttc
     1321 cgctggcgcc accacctcga ggcggtgctg gcccacggca cgccgcccac gccggtcatc
     1381 cgcgacatcc tgttcgggga cacctgagcg gatgggccga ggcttcgggg ccctccgcca
     1441 ccgcaacttc cgcctctact ggtccggcca ggtcgtcagc ctggtcggga cctggatgca
     1501 gagcgtctcc cagccctggc tcgtcctcga gctcggcggc tccgcgctcc aggtgggcac
     1561 ggtcattgcc ctccagttcg cgccggccct gttcctggcg cccatcggcg gcgttctggc
     1621 cgatcggttc gacaaacgcg ggctcctcct cgtcacccag gccctcgcca tgctccaggc
     1681 ggccactctc tttgggctga ccttcagtgg cgggatcgag atctggcaga tccaggtcct
     1741 ggccctgatc ctgggcatca ccagcgcatt cgacatgccg gtccgccagt cgttcgtggc
     1801 cgaggtcgtg ccgcgccgcg atctgctgca ggcgatcgcc ctgaacagcg cctcgttcaa
     1861 cctggcccgt gtcgtcggtc ctgcggtggc cggcatcacc ctcgccctgt tcggcagcgc
     1921 gttcaacttc ggaatcaaca ccctgacgta caccgcggtc ctggtcgggc tgctgctcat
     1981 cgacccgaag accctcatcc gggtcccgat ccctgcgatc cagcccagca tccggacctc
     2041 gctgcgcgag ggcttccagt acgcggcgca gactccgagc gtcctgtggc tgctcgtcct
     2101 gctgggcggg atgagcgtgt tcgcgatgaa cttccagacc ttgctcccgc tcttcgcccg
     2161 gttcacgctg gacatggatg ccggcgccta cggagcgctg ttcgcggtga tgggcgctgg
     2221 gtcgctggtc ggttcgctgg tcctcgcgtt catgggtgaa agccggccgc ggctacgcct
     2281 gatcctgggc ggcggcgtgg cgttcctcgt gttcgaggtc atgctcggcc tgatccggac
     2341 tcccgctctg gcctaccccg tcgtcgccct cgtcggcctg gcgtccatgc tcatggtcaa
     2401 cacgatcaac gccaccgtcc agcgctcggt gccggacgag ctgcgcggcc gggtgatggc
     2461 cctgtacgtg accgtgttcg cggggtccgg gccgctgggc ggcctgttcg ccggcgcggt
     2521 ggcccaggtc ctgggcccgc ccgcgggatt catcatcggc gcggccctgg cgtccatctt
     2581 cctggccctc acctggtgga agctcggtcg ccgtgggccc atgccactga cggcgccgac
     2641 gccggcctga ccgcgaccga ccgaccaatc caaccgctgt gaccgcgccc gccaccgctg
     2701 ccgagtcgaa ccgcgcgaac ctgaccctgg gtctcctggc cctcgcccac tcgctcatcc
     2761 acatgcagtc ggcggtgatg ccgctggtgt acatcgcggt catcgaccag ttcggcctga
     2821 acgaggcgtc catcggcttg ttcatcgcgg tcacgaccgc ggtcgccggc gccatgcagc
     2881 tgtcgtatgg gttcctgacc cgctacatcg cgcgcccggt gctgatcggc gtcggccagc
     2941 tcgtgttcgg tgtcagcctc atgctggccg ggttcgcgac caccatcggc cagctgctgg
     3001 ccacgatcag cggcgcccgg gtgggcgcca gcccgcagca tccggtgggc aacgggatcc
     3061 tgtccgatgc gttcccgccc gagcggcggg gattcgcgat cagcacccac atcgccggcg
     3121 gcaacgtggg caccatcctg gtcccgttca tcggcggcgc ccttctcgcc tccgtgggct
     3181 ggaacctgac cctggtcgcg ttcgggattc ccgcactcct cgtcggcgtc ctgatcctgt
     3241 ggttcgtccg cgaggactcc cggacgtacc gccgtgatgc ccgggccgcg ggcagctggt
     3301 cgagccacgt ccgggcggtg tttcgtcggc gagacctcat gaagatcctg gccgccgcga
     3361 tggtcgcggc cggcggtcgt ggtctggaca tcgcggcgcc gttcctgctc ctgtacttcc
     3421 gctccgagct cggcatcgac gacaacaccg tcctgtggct gtatgccctg ctcctggtcg
     3481 gagcagtcgt cggcccggtc ctggccggca tcctgtcgga ccgatacggc cggcgccgga
     3541 ccctgatcgt ctactacgtc ctgtcctcgg tcgggatcct gtccgtcgtg ctggtcggcg
     3601 gcaacctcct gctcctcgca ccactcctcc tgccgttcgg gactgcagtt ttcagcgagg
     3661 gcccggtcct ccaggcgttc ctcgcggatc gggcggtcgg ccccatccgc gacgtcgcgt
     3721 tctcgatcta cttcaccgca gcctttggaa tcggtgcgtt gtgggcgctg atcatcggcg
     3781 ccatcgtggc cggccccggc tacgtgaccg cgttcgtggt catggccgtg tcctacatcg
     3841 cgggcgccat cctcatctcg ctcgttcggg atcgaccacc ggatgtcggg ctggacgccg
     3901 ccggcaccga gcctgcctag gcgggttggc cggtcttcct ccgggacagc cggttggcct
     3961 ccttctcgaa gcggttgccg cggtaggcgt tctggccgtg gaggagagcc gacaggctcg
     4021 aggcgacgta ggcaaccggg aagacgatcc cccagcgctc gtactgcgcg acgtggaccc
     4081 gttcgtgccg gcggctgacc tcgagtgccc gcgcattggc ccccagcacg acgtggccga
     4141 gggtcagcgc gcccgccccg ccatgcccga ccggcaccac cttcagcccg cggcgaatga
     4201 cgccgccgta cccctcgaac gctccggaca cgaatgccac gtgaccgccg gtgagggcgc
     4261 ccgcgaccag gatcgcgacc ccgaacaggg tcacgggcag cgcccagatc aggcccaggg
     4321 gccacggcag gcgaagaccg tcgcgcatgg gcggcagggt agccgacggc tagaatccgg
     4381 ccgagaaccg atcgtgccac tgcccgtcac aggagcgccc ctccgatgac ctccgactcg
     4441 gccaccatcg agaacctcct ccgcgaggac cgcaccttcc cgccgccgcc cgcctttgcc
     4501 gcggccgcca tcgcgaacga ccccgacatc tacgagcgca cggccaccga cgaggggttc
     4561 gaggcgttct gggccgagga gtcgcgccgg atcgactgga tcgagccgtg gcagcggctt
     4621 ctgaagtggg aagccccgtt cgccgagtgg ttcgtgggcg gcaagctgaa cgtgagcgtc
     4681 aactgcctcg accgccacgt ggccaacggc ctcggggaca aggttgcgta ccactgggag
     4741 ggcgaacccg gcgaccaccg gacgatcacc tacgcccagc tgctggccga gacctgccag
     4801 atggcgaacg ccctccgtga gctcggcgtc aagaaaggca accgggtcgc gatctacatg
     4861 ccgatgatcc ccgagctgcc ggtggcgatg ctggcctgcg cccggatcgg ggcgccgcac
     4921 agcgtcgtgt tcggcggatt cagcgcggac gccctggccg gccggatcga cgatgcgcaa
     4981 tgcacgacgg tgatcaccgc cgacggcggc taccgcaaag gcgcgctcgt gccgctcaag
     5041 gacaacgcgg acgacgccat gggccggtcg ccctcggtcg agcactgcat cgtcgtccgg
     5101 cgaaccggcc aggacgtggc ctggcaggaa ggccgg
//
LOCUS       DASYGU010000251         7790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1790016, whole genome shotgun sequence.
ACCESSION   DASYGU010000251 DASYGU010000000
VERSION     DASYGU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790016"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VGO32_07115"
     CDS             complement(<1..1358)
                     /locus_tag="VGO32_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV7200557.1"
                     /translation="MTQSPPTYRVARRPSAALAAALSFVLPGLGQAYAGRTRLGLLFA
                     APVLILAGMVLGAVLLGTDVILRTLVIPGALVAVLALDVGLMLWRLVSIGEAGLAQPR
                     GRSPFTVALVAILMVATVAMHLWVANVTVAADRALAQVFDPPAGAGYTPPAFGHLDPD
                     VPEYRWDGTERVNILLIGYDSGPGRVDENTDTLMVATIDPASRTAALISIPRDTGYVP
                     LPDRRVYADGVYPRRVNELASAANADPALWCPDLAEGDDCGLATLRSAVGLYLGIEIH
                     HVAWVDLMGFAALVDAVGGVELCLPGVLADPRYGGPTWEGRGIVLEGGCRVYDGPHAL
                     AFARIRQGTLTLPDGTVQEQNDFLRAARQQEFLLAFQQQVAKTNLLISFPSLLNAVSE
                     TVTTDFPRAQAGDLASLAPLITSDNIQRVVLGWPEYVDLPVDPLNYYLLIPRRDAVRD
                     LMA"
     gene            1529..2806
                     /locus_tag="VGO32_07120"
     CDS             1529..2806
                     /locus_tag="VGO32_07120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7200558.1"
                     /translation="MTTELIFLLVSAFVAGLIVTAGFVAVLSARRLGHTEGGMLPVGP
                     GPGMEPPVRDKYLSTTESTGSPLAWIGPNRFRATIRACWWATIAGVLIAVGVTNVYSE
                     SQTAIYALGALAVIAVVTFHEVIPDRWRTWIVSAAEVATAIALLGALVFLTGRASSPF
                     APLLTLPVLGVALGGRPAAGLSAAAAASLTFAVVLATGPDLPFAPGELLPAVVVLGVV
                     WLGTVSAVVFAAQQRRLQAATLQISVTDSLTGLFNRAQIYVDLDQEVRRSRRSLRPFC
                     LLMVDMDGLKAINDSLGHERGDAAIRGIASVIRRSIRTVDSAYRYAGDEFVVLLPETD
                     FAGAFVVAEKVRQGAEELTAVVTREEAPTTVSIGLVSHPEDGSTDEELMLAVDRAMYM
                     AKASGKNQVSGYGRPSKQSATSAGTVSRLSALG"
     gene            2882..4246
                     /locus_tag="VGO32_07125"
     CDS             2882..4246
                     /locus_tag="VGO32_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV7200559.1"
                     /translation="MNQAAASRPDPRAPLAAFFSFVLPGLGQAYNRQFRLALLLGLPA
                     AILGWLVIAAAGRGSNVLTQLLDVRVLWAIVVLNLAFLAWRAVAIYQAHAAREAPSWR
                     QWTTWITAGLLAVTLAMHLIPAWYATATIDTLAAVGLGGGGAGGILGTPKDVLEAGPS
                     GTPKPAPWEEPEATPVGLTGRFTVLLVGVDLGPGRGTYNTDTMMVATIDPRSGASLIS
                     IPRDTFGTPLGDGRIYNAKLNSLLAYARLDGGTYPLGGPETIKAAVGALLGIQIDYIG
                     AVDFLGFTSAVDAVGGVDITVTRPVHDFGYRDEYNNLVGFHIEPGTYHMNGYTALAFA
                     RSRKGAGDNDYTRADRQQQVIAAMVNKMKAGAWLLNLPSLLQAIGDSVATDIPVELMP
                     HIAHALLDADLSKIDRLVIQPPLVQSGFLTDGTYILTPDIPGIRAAVHHLVTVDAGPT
                     PPPD"
     gene            4282..5457
                     /locus_tag="VGO32_07130"
     CDS             4282..5457
                     /locus_tag="VGO32_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7200560.1"
                     /translation="MPGFSTRAIKAAGAVEKIEQTPVSVPIYQTSTFEVGSAEELEEL
                     LEFRRPGHSYTRYSNPTHAALEGAVAELEGAEAGLATASGMAAVHGAMLSLIGSGDEV
                     ILPAAVYGGTVGLFNAVMPRLVITKRHVRSGDTAAVIEAIGPTTRLVWLETIANPTTE
                     MPDIAAIAEAAHERGVHVAVDNTFASPYLCNPLALGADLVVHSLTKYIGGHSDLIGGV
                     VVGSAERIAAVREVVINTGGNANPFEAFLALRGLKTLAVRMDRHSDNGLAVARGLEGQ
                     PGIERVLYPGLPSHPQHELARRSLRDGRAGGMMAVELSGGRTAGERFLGRIQVARHAT
                     SLAGVESLISHPASSSHRQYVDAELTAAGLSPGMLRVSIGLEDADDLVTDLAAAAAG"
     gene            5466..6533
                     /locus_tag="VGO32_07135"
     CDS             5466..6533
                     /locus_tag="VGO32_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7200561.1"
                     /translation="MPRLIDLWRAVDPEARLVAGEPTATERLVRGISRTRAAPPHLPP
                     PADGHLIIVDAGLLDALPPARLLAGVAASGSTPAGLLVATGARDRRDVWPDETPPILL
                     STRTASVLEETAAAYMADESGALSRFATDLRLAVAEAALAEPAPATAAAEVARRIRRG
                     VAVVADGEMVALVPRPAGRALAARFAAAFSRLFGTPSARAAASRRLRDGLWIHEQRIR
                     PGAAVWVFDDMPLSRLDEVAAESLAMTLRALLLRPAVRPRRPSARIHDSSPRRSPAAG
                     PVDLVREPETLDATLLAVARANGRVAPAARSLGVHRNTVLYRLRIARDQRGIDPRRPE
                     DALRLLRAAERDRERGGDRSA"
     gene            6696..>7790
                     /locus_tag="VGO32_07140"
     CDS             6696..>7790
                     /locus_tag="VGO32_07140"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV7200562.1"
                     /translation="MARKELTAALELAEEKNLRFVSLQFTDIVGHVKSVQVPMHQLEE
                     AVEHGKWFDGSSIEGFARIAESDMFLVPDLSTFSPIPWEPGTDADGRAIATGSARVIC
                     DVFTPNGDPFPGDPRWVLRRQIQKAKDLGYTFNTGPELEFFLLRLGDGRVEPLPHDAA
                     GYFDLSADLATDVRKEMMNDLEAIGIEVETAHHEVAIGQHEIDFKYADALRTADNAVT
                     FKTTLKAVAQRHDLHATFMPKPFFGINGSGMHTHQSLWDDKRGKNAFADAKDPYGLSA
                     MAKHYIAGTLEHARGMIAVLAPLVNSYKRLVPGYEAPVYIGWARINRSALIRIPQISS
                     GQLDSTRIELRCPDPSSNPYLAFAAMLAAGL"
BASE COUNT         1173 a         2790 c         2651 g         1176 t
ORIGIN      
        1 gccatcaggt cacgcaccgc atcccgacga gggatcagca ggtagtagtt cagcgggtcg
       61 accggtaggt ccacgtactc cggccagccg aggacgaccc gctggatgtt gtccgaggtg
      121 atgagcggcg caaggctggc caggtcgccc gcctgggctc gcgggaagtc agtcgtcacc
      181 gtctccgata ccgcgttcag caggctgggg aacgaaatca gtagattcgt cttcgccacc
      241 tgctgctgga aggccagcag gaactcctgc tggcgcgccg cgcgcaggaa gtcgttctgc
      301 tcctgcacgg tgccgtcggg gagggtgagc gtgccctgtc ggatccgggc gaatgccagg
      361 gcgtgggggc cgtcgtacac gcggcaacca ccctccagca cgatgcctcg tccttcccat
      421 gtcgggccgc cgtaccgggg gtcggccagg acaccgggca ggcacagctc aacgccgccc
      481 acggcatcga cgagcgcggc gaagcccatc aggtccaccc aggcaacgtg atggatctcg
      541 atgcccaggt acaggcccac ggccgagcgc agggtcgcga gcccacagtc atccccttct
      601 gccaggtcgg ggcaccagag ggccggatcg gcgttggccg cggacgcgag ctcgttcacc
      661 cggcgggggt agacgccgtc cgcgtagacg cgccggtccg gaaggggaac gtacccggtg
      721 tcacgcggga tgctgatcag ggcggccgtc cgggaggcag gatcgatggt cgccaccatc
      781 agggtgtcgg tgttctcgtc cacccgtccc gggccgctgt cgtagccgat gagcaggatg
      841 ttcacccgct cggtgccgtc ccaccggtac tcggggacgt ccgggtcgag gtgcccgaac
      901 gcggggggcg tgtaacccgc gcccgccggc gggtcgaaga cctgcgccag tgcccggtcc
      961 gcggcgaccg tgacgttggc cacccacagg tgcatggcga cggtcgccac catcaagatc
     1021 gccaccaggg ccaccgtgaa cggcgaccga ccgcggggct gggcgaggcc ggcctcgccg
     1081 atcgagacca gccgccagag catcaagccg acgtccaggg cgaggaccgc gaccagcgcc
     1141 cccgggatga ccagcgtccg gaggatgaca tcggtgccta ggagcaccgc tcctagcacc
     1201 attccggcca ggatgaggac cggtgccgca aagagcagac cgaggcgagt ccgtccggcg
     1261 taggcttgac cgagtccggg aagcacgaac gagagcgccg cggcaagcgc ggcgctgggt
     1321 cggcgggcca cccggtacgt tgggggcgat tgagtcacat ggacaccatg aagcagggtc
     1381 ggcaaccagg gcgggacggc gagtatagca atccggtcgg atctccccgg aagtgttgcc
     1441 tgtgggcacc agtagggtgg tacggacggg ttagtacctt tgcgcccgtc cgtgggccgc
     1501 tcctagactg tggcgaccga gagggagcgt gacaaccgag ctgattttcc tccttgtgag
     1561 tgcgtttgtg gccggcttga tcgtgaccgc cggcttcgtt gctgtgctct ccgcccggcg
     1621 cctgggccat accgaaggcg ggatgctgcc ggtcggcccc ggccccggca tggaaccgcc
     1681 ggtgcgcgat aagtacctct ccacgaccga atcgaccggt tcgccgctgg catggatcgg
     1741 gccaaatcgg ttccgggcca ccatccgggc ctgctggtgg gcgacgatcg ccggcgtcct
     1801 catcgcggta ggggtgacga acgtctacag cgagagccag acggccatct acgccctcgg
     1861 cgcgttggcc gtcatcgcgg tggtgacgtt ccacgaggtc atcccggatc gatggcggac
     1921 gtggatcgtg tccgccgccg aagtggccac cgcgatcgcc ctgctgggag ccctggtctt
     1981 cctcaccggc cgcgcgtcct ccccgttcgc gccgctcctg accctccctg tgctgggcgt
     2041 cgcgctcggt ggccggccgg cggcggggct ttccgccgcc gccgccgcca gcctgacctt
     2101 tgccgtcgtg ctcgccaccg gacccgacct cccgtttgcc cccggcgagc tgctgccggc
     2161 ggtggtcgtg ctcggcgtcg tgtggctggg cacggtctcg gcggtcgtct tcgcggctca
     2221 gcagcgccgc ctgcaggcgg caaccctcca gatctcggtc accgactccc tgaccggtct
     2281 gttcaaccga gcccagatct acgtcgacct cgaccaggag gtccggcgca gccggcgttc
     2341 gctccggccg ttctgcctgc tgatggtgga catggatggc ctgaaggcga tcaacgactc
     2401 gctcggccac gagcgcggcg acgcggcgat ccgcggcatc gcgagcgtca tccgccggtc
     2461 catccggacg gtggactccg cctaccgcta cgccggcgac gagttcgtcg tgctgctgcc
     2521 cgagaccgat ttcgcgggag cattcgtggt ggccgagaag gtgcgccagg gcgccgagga
     2581 gctcaccgcg gtcgtgaccc gcgaggaagc gccgaccacg gtcagcatcg gtctggtatc
     2641 acaccccgaa gatgggtcga ccgacgaaga gctcatgctg gccgtcgatc gcgcgatgta
     2701 catggccaag gcgagcggca agaaccaggt cagcgggtac gggcgaccga gcaagcagtc
     2761 cgcgacgtcg gcggggaccg tcagccggct ctcggcactg gggtagttgg cggggctccg
     2821 gtatactcgc gcgccgccag gtggttcgat cgaacacccg gcggatgacc atggaggggc
     2881 tgtgaaccag gctgctgcca gcaggcctga tccccgcgcg ccgctggctg ccttcttctc
     2941 gttcgtgctg cccggactgg gccaggccta caaccgccag ttccgcctcg cgctgctcct
     3001 cggcctgccg gccgccatcc ttggctggct ggtcatcgcc gctgcggggc ggggcagcaa
     3061 cgtgctgacc cagctgctcg acgtccgcgt cctgtgggca atcgtcgtcc tcaaccttgc
     3121 cttcctcgcc tggcgggcgg tggccatcta ccaggcccac gcggctcgcg aggcgccgag
     3181 ctggcggcag tggacgacct ggatcaccgc cgggctgttg gccgtgacgc tggccatgca
     3241 tctcatcccg gcctggtatg cgaccgcgac gatcgatacc ctggccgcgg tcggccttgg
     3301 cggcggggga gccggcggga tcctgggcac gcccaaggac gtcctcgagg ccggccccag
     3361 cggcacaccg aaacccgctc catgggagga gccggaggcg accccggtcg gtctgaccgg
     3421 tcggttcacc gtcctgctgg tcggcgtcga cctcggcccg ggtcggggca cgtacaacac
     3481 cgacacgatg atggtggcca ccatcgaccc gcggtcggga gcctcgctta tctcgatccc
     3541 gcgcgacacg ttcggaaccc cgctcggcga tggtcggatc tacaacgcga agctgaactc
     3601 gctgctggcc tacgcccgcc tcgacggcgg aacctacccg ctcggcggtc ccgagacgat
     3661 caaggcggcg gtgggcgcgc tgctcgggat ccagatcgac tacatcggtg cggtggactt
     3721 cctgggcttc accagcgccg tcgatgccgt cggcggggtc gacatcaccg tcacccgacc
     3781 ggtccacgac ttcggctacc gcgacgagta caacaacctc gtcggattcc acatcgagcc
     3841 cgggacgtac cacatgaacg ggtacaccgc gctcgccttc gcccggtcgc ggaagggcgc
     3901 cggggacaac gactacaccc gagccgatcg gcagcagcag gtcatcgcgg ccatggtcaa
     3961 caagatgaaa gccggcgcgt ggctgctgaa tctcccctcg ctcctgcagg ccatcggcga
     4021 ctcggtcgcg accgacatcc cggtcgagct catgccgcac atcgcccatg cgctgctcga
     4081 cgccgacctg tccaagatcg atcggctcgt catccagccg ccgctggtcc agtccggctt
     4141 cctgaccgac ggcacctaca tcctgactcc ggacatcccc ggcattcggg ccgccgtcca
     4201 tcacctcgtg accgtggacg cgggccccac gcccccgccc gactgagcag cgcttcggca
     4261 acggccgggt agactctgcc gatgcccggt ttctcgacgc gagcgatcaa ggccgccggc
     4321 gcggtcgaga agatcgagca gaccccggtt tcagtcccga tctaccagac cagcacgttc
     4381 gaggtggggt ccgccgagga gctcgaggag ctgctcgaat tccgtcgtcc gggccactcg
     4441 tacacccggt actcgaaccc gacccatgcc gccctggaag gcgcggtggc cgagctcgag
     4501 ggggccgaag cgggcctggc caccgcgtcc ggcatggctg ccgtccacgg cgcgatgctg
     4561 tccctgatcg ggagcggcga cgaggtgatc ctccccgccg cggtctacgg cggtaccgtg
     4621 ggcctgttca acgcggtcat gccccgcctg gtcatcacca agcgccacgt gcggagcggc
     4681 gacacggcgg ccgtgatcga ggccatcggc cccacgacca ggctggtatg gctcgagacg
     4741 attgcgaacc cgaccaccga gatgcccgat atcgcggcca tcgccgaggc cgcccacgaa
     4801 cgcggcgtac atgtcgcggt tgacaacacc tttgcgtcgc cgtacctgtg caacccgctc
     4861 gcgctcggtg cggatctcgt cgtccattcg ctcacgaagt acatcggcgg ccactcggat
     4921 ctgatcggag gcgtggtcgt cggcagtgcc gagcggatcg cggcggtccg cgaggtcgtg
     4981 atcaacaccg gcggaaacgc gaacccgttc gaggcgttcc tcgccctccg cggtctcaag
     5041 acgctggcgg tccgaatgga tcgccattcg gacaacggcc tggccgtggc acgtggtctc
     5101 gaggggcagc cgggcatcga acgggtcctg tacccggggc tgccctccca cccacagcac
     5161 gagctcgcca ggcgcagcct gcgagacggc cgggccggcg gcatgatggc cgtggagctg
     5221 tccggcggaa gaacggctgg ggagcgattc ctgggaagga tccaggtcgc ccgacacgcg
     5281 accagcctgg ccggggtcga aagcctgatc agccaccccg cctcctcctc ccatcgccag
     5341 tacgtcgacg ccgagctgac tgcggccggc ctgtcgcccg gcatgctccg ggtgtcgatc
     5401 gggctcgagg acgcggatga cctggtgacg gacctcgcgg ccgccgccgc cggctgacac
     5461 cgagcatgcc tcggctcatc gacctctggc gcgcggtgga cccggaggcg cgcctggtgg
     5521 cgggggagcc aacggcgacc gagaggctgg ttcgggggat ctcgcggacg cgggcggcgc
     5581 cgccccatct cccgccgcct gccgatgggc acctgatcat cgtggatgcc ggcctgctcg
     5641 acgcgcttcc gcctgcccgc ctgctggccg gggtggcggc ctccggaagc accccggccg
     5701 ggctgctggt cgcgaccgga gcgcgcgatc ggcgcgacgt ctggcccgac gagaccccgc
     5761 cgatcctgct cagcacccga acggcctctg tcttggagga gaccgcggcc gcctacatgg
     5821 cggacgagag cggcgcactc tcgcgcttcg cgaccgatct ccgtctcgcg gtggccgagg
     5881 cggcgctggc cgagccggca ccggcgaccg cggcggccga ggtcgcgcgc aggatccggc
     5941 gcggcgtcgc ggtggtcgct gacggcgaga tggtcgctct ggttccgcga cccgcgggcc
     6001 gagccttggc cgcccgattc gccgccgcct tctcgcgcct gttcggaact cccagcgctc
     6061 gcgcggcggc atcgagacgc ctccgggacg ggctctggat ccacgagcag cgcatccggc
     6121 cgggcgccgc ggtctgggtg ttcgacgaca tgcccctgtc ccggctcgac gaggtcgccg
     6181 ccgagtcgct ggccatgacc ctccgtgcac tcctgctccg ccccgcggtc cggccgcggc
     6241 gaccctcggc ccgaatccac gactcctcgc cccgccggtc tccggccgct ggcccggtcg
     6301 acctcgtccg cgaaccggaa acgctggacg ccactctgct ggccgtggcg cgcgcgaacg
     6361 gacgagttgc gcccgccgcg cggagcctgg gcgtccatcg caacacggtc ttgtatcggc
     6421 tgcgcatcgc ccgcgaccag cggggaatcg acccccgacg tccggaggat gcccttcggc
     6481 tgctgcgcgc tgcggagcgg gatcgcgagc gcggaggcga ccgctcggcc tagccccttg
     6541 ctttgtgcag agtgcacaaa gaatcgcccg tccaccgctc ttcgcggtgc tccccgcgga
     6601 cgctcctccg ccctaggctg gccgtggccc gcgacgcccg gtgggcacgt catcggttcg
     6661 gcgttcgtat cggtctgaga ccggaaggga attccatggc tcgaaaggaa ctcacggccg
     6721 ccctggagct cgcggaggag aagaacctcc gcttcgtcag cctccagttc accgacatcg
     6781 tcggccacgt gaagtcggtc caggtaccga tgcaccagct cgaggaggcc gtcgagcacg
     6841 gtaagtggtt cgacggcagc agcatcgagg gctttgcccg gatcgcggag tcggacatgt
     6901 tcctcgttcc cgacctgtcc accttctcgc ccatcccgtg ggagcccggc acggacgcgg
     6961 acggccgggc catcgcgact ggcagcgccc gggtcatctg cgacgtgttc acccccaacg
     7021 gcgacccgtt cccgggcgac ccgcgctggg tgctccgtcg ccagatccag aaggcgaagg
     7081 accttggtta cacgttcaac acgggtcccg agctcgaatt cttcctgctc cgcctgggcg
     7141 acggacgggt cgagccgctg ccccacgacg cagccggcta cttcgacctc tccgccgacc
     7201 tggcgaccga tgtccgcaag gagatgatga acgacctcga ggccatcggg atcgaggtcg
     7261 agaccgccca tcacgaggtg gcgatcgggc agcacgagat cgacttcaag tacgccgatg
     7321 cgcttcgcac cgccgataac gcggtcacct tcaagacgac cctgaaagcc gtcgcacagc
     7381 gccatgatct gcacgccacg ttcatgccca agccgttctt cgggatcaac gggtccggca
     7441 tgcacaccca ccagtcgctg tgggatgaca agcgcggcaa gaacgcgttt gccgacgcca
     7501 aggacccgta cggcctgtcg gcgatggcca agcactacat cgccggcacc ctggaacatg
     7561 cccgcggcat gatcgcggtc ctcgccccgc tcgtgaacag ctacaagcgg ctggtgccgg
     7621 gatacgaggc gccggtgtac atcggctggg cgcggatcaa ccgatcagcg ctgatccgca
     7681 tcccccagat ctcgagcggc cagctggact cgacccggat cgagctccgc tgccccgacc
     7741 cgtcatccaa tccgtatctc gccttcgcgg ccatgctcgc cgccggcctg
//
LOCUS       DASYGU010000252         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_92287, whole genome shotgun sequence.
ACCESSION   DASYGU010000252 DASYGU010000000
VERSION     DASYGU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92287"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /gene="hrcA"
                     /locus_tag="VGO32_07145"
     CDS             complement(<1..764)
                     /gene="hrcA"
                     /locus_tag="VGO32_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV7200563.1"
                     /translation="MLRAVVEDYVLTAVPVGSQALVQRYGLQVSPATVRSAMADLESL
                     GLLTHPHTSAGRVPSDLGYRYYVETLMRESELDRADALMIRHQFSQVQLTSNDWLRLA
                     ASTLAASTRAAAVVTPARSRRAKFAHLQLVDLADHARMAVVVLTDGTVVERHLDPSAI
                     NRVAGGPAGQEDLDNAAAELNAELKGLTSPQARRRGRRLTPLAAHVAAAVAEILEEAD
                     GSSIEEVYTDGLAYVLDQPEFAGGTALRPVLEILQHV"
     gene            complement(1042..2517)
                     /locus_tag="VGO32_07150"
     CDS             complement(1042..2517)
                     /locus_tag="VGO32_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200564.1"
                     /translation="MRVKVGSVAFLAAALIGAVIGALLAFVPAANAWAPVWAAAVDPA
                     LARPPAAELYRMAADGSGQARLTISDVDERSPAVSPDGTRVAVKLWPEEGEVPQTLAV
                     IDLHTGERVDLLTEGIVNQPSWSPDGERVLFGWNPDNAVFQLWWIAADGSAPPERLMD
                     SEADSYAGQWSPDGERLLFSSDRDGFGIYVANADGTDAHKIVDHPRYADAAWSPDSQL
                     IAFTSDEEGDFGLSVVAPDGSGLRLVHVVPGAVLIPDWSPDGQTLVYESHQDGADEVD
                     SVALDGSHEQNLTRNPGSRDAMGGPTVARDGQIIYAAASYQSAAVSPFVRERLGLAAL
                     VVQSVLLALGAALLFVGNAVRPGSLTVLVGAATVVALALGESAAWAFLPAALLAGVAA
                     DIAWLALRTRTPPRLARATAVGVLTGVYVAGHLATVAWYGITQWPAGWLQGEPWRVSG
                     VGYAPEFVALLVLVATGIGVVIGLVTGASEPVAVGGGLSSA"
     gene            complement(2608..>3638)
                     /locus_tag="VGO32_07155"
     CDS             complement(2608..>3638)
                     /locus_tag="VGO32_07155"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200565.1"
                     /translation="GRALLRAGLDTAVGPGRGGQDRQAGVAILLDAREELRAAGDQAH
                     EADVMYYLGFGGLLDGNLPAALSAWQDAAALAHQAGDAGREVRAQQRVIHTLFDAGRR
                     DEAEQLLRQAVERAPELSIATRAQVWKSQGQYLTRYGIDVEEGRALLQRALEVGEQFG
                     DFDLRAGSLAGLAEIDVITGAAADALRWSQAHLDLVTALGHDWMLAAAEHWMAQSLLA
                     AGDASAAEAHARRATTLATGDDPSVAANAQLDLARVYDALGRAGDASEVFDGATETFA
                     SLPFKVDVASFDLARGAFHIANGRADEGEAHVARARTAYEAFLGPQTPFLAYADRIVA
                     SAQERAAH"
BASE COUNT          569 a         1245 c         1325 g          499 t
ORIGIN      
        1 acgtgctgga ggatctcgag gaccggtcgg agggcggtgc ccccggcgaa ctcgggctga
       61 tcgaggacgt acgccaggcc gtcggtgtac acctcttcga tcgaggagcc atccgcctct
      121 tcgagaatct cggctactgc ggcggcgaca tgggcggcga gcggagtgag cctgcggccg
      181 cggcggcgcg cctggggact ggtgaggcct ttgagctcgg cgttcagctc tgcggccgcg
      241 ttgtccaggt cctcctggcc ggcgggaccg cccgcgacgc ggttgatcgc cgaagggtcg
      301 agatggcgtt cgacgacggt gccgtcagtc agaacgacga cggccatccg ggcgtggtcg
      361 gccaggtcga cgagctggag atgggcgaac ttcgcgcgac gcgagcgggc cggcgtgacg
      421 accgcggccg cgcgggtgga ggcggcaagg gtcgatgccg ccagccgcag ccagtcgttg
      481 gaggtgagct ggacctggct gaactggtgg cgaatcatga gcgcatcggc gcggtcgagc
      541 tccgactcgc gcatgagggt ctcgacgtag tagcggtagc ccaggtccga cgggacgcgg
      601 ccggccgagg tgtgggggtg ggtcaggagg cccagggact cgaggtcggc catcgcgctg
      661 cgaaccgtgg ccgggctgac ctgcaggccg tagcgctgga cgagggcctg ggaaccgacc
      721 gggacggcgg tcaggacgta gtcctcgacc acggcacgga gcacggcacg ctgccgctcg
      781 gtcagatcaa ccggcggatg ggacggcgaa gatgattgtg gcgttcggtc cggcatttgt
      841 gctcaatagg ttagcactct catggttaga gtgccaattc gaccgggatt gatcgtactg
      901 cgagcgccaa atgcctgtca acgcgagccg ggggtattgc gagaggcccc tcactgaaca
      961 cctgccccga cgggtggagg ccccgaccac gatttagccc gggtcgactg aacacccggg
     1021 cgcgcctgag tttcgcctcg ctcaggcgct ggacaagccg ccgccgaccg cgactggttc
     1081 cgacgcgccg gtcacgagac cgatgacgac acctattccg gtcgccacga gcaccagcag
     1141 ggcgacgaac tccggcgcgt agccgactcc cgaaacgcgc cacggctcac cttgtaacca
     1201 gccggccggc cattgggtga tcccgtacca ggccacggtc gcgaggtggc cggcgacata
     1261 cacgccggtc agcacgccaa cagcggttgc gcgggcgagg cggggcggcg tccgggtccg
     1321 tagcgccagc cacgcgatgt cggcggccac gccggccagg agagccgcgg gcagaaaggc
     1381 ccaggccgcc gattcgccga gggccagggc gacgacggtc gcagccccga ccaggacggt
     1441 cagggacccc gggcgaaccg cgttgcccac gaacaggagt gctgcaccga gagccaggag
     1501 cacgctctgg acgacgaggg ccgccaggcc aaggcgctcg cgaacgaagg ggctgactgc
     1561 ggccgattgg tacgaagccg cggcgtagat gatctggcca tcgcgggcga ctgtcggccc
     1621 acccatcgcg tcccgggagc ccggatttcg agtgaggttc tgctcatgtg acccgtcgag
     1681 cgccacggaa tcgacctcgt cggcgccatc ttggtggctc tcgtagacca gggtctgtcc
     1741 atcgggcgac cagtcgggaa tgagcactgc cccgggaacg acgtgaacga gccgcagccc
     1801 ggagccgtcg ggggccacga ccgagagccc gaagtcccct tcctcgtccg aggtgaaggc
     1861 gatgagctgg ctgtcgggtg accaggcggc atcggcgtac cgtgggtgat cgacgatctt
     1921 gtgcgcgtcg gtcccgtcgg cgttcgcgac gtagatgccg aacccgtcgc ggtccgaaga
     1981 gaacaggaga cgctccccat ccggcgacca ctggccggca tacgagtccg cctcgctgtc
     2041 catcaggcgc tccggcggcg cggagccgtc ggccgcgatc caccacagct ggaagacggc
     2101 gttgtcgggg ttccagccga acaggacccg ctctccgtcc ggcgaccagc tgggctggtt
     2161 gacgatgccc tcggtcagga ggtcgacacg ctcgcccgtg tggaggtcga tgaccgccag
     2221 ggtctgcggc acttccccct cctcgggcca cagcttgacc gcgacccgcg tcccgtcggg
     2281 tgacacggcg ggcgaccgct cgtcgacgtc gctgatggtc agccgcgcct ggcccgatcc
     2341 atccgccgcc atgcggtaca gctcggcagc cgggggtcgc gcgagagccg ggtcgacggc
     2401 cgcggcccag accggggccc aggcattcgc ggctggcacg aaggccagca gtgcgccgat
     2461 gaccgccccg atcagcgccg ccgccaggaa cgcgaccgag ccgaccttga cccgcacgcc
     2521 gcgagcctag caccaagcat tcggcgtccg gaagcccgcc ggtgttcagt cgacccgctc
     2581 cccctatacc catcaacgcg gcctcattca gtgagcggct cgctcctgcg cgctggcgac
     2641 aatgcggtcg gcgtaggcga ggaagggggt ctgcgggcca aggaaggcct cataggcggt
     2701 gcgggcccgg gcgacgtgcg cctcgccctc gtctgctcgg ccgttggcaa tgtggaaggc
     2761 gccgcgggcc aggtcgaagg aggcgacgtc caccttgaac ggcagcgatg cgaaggtctc
     2821 cgtcgccccg tcgaacacct ccgaggcatc tcccgcacgg ccgagggcgt cgtacacccg
     2881 ggccaggtcc agctgggcgt tggccgcgac cgacgggtcg tcgccggtgg ccagggtcgt
     2941 tgcccgccgg gcgtgtgctt cggcggccga cgcatcaccg gcggcaagga gcgactgggc
     3001 catccagtgc tccgccgccg ccagcatcca gtcatgaccg agcgctgtca cgaggtcgag
     3061 atgcgcctgc gaccagcgga gggcatcggc cgcggcgccg gtgatgacgt cgatctccgc
     3121 caacccagcc agcgagccgg cgcggaggtc gaagtcgccg aactgctcgc ccacttccag
     3181 tgctcgctgg agaagggcgc ggccctcctc cacgtcgatg ccatatcggg tcaggtattg
     3241 gccctgcgac ttccagactt gcgcacgcgt cgcgatgctc agctcaggag cgcgctccac
     3301 ggcctggcgg aggagctgct ccgcctcatc gcggcggcca gcgtcgaaca acgtgtggat
     3361 gacccgctgc tgggcgcgaa cctcgcggcc cgcatctccg gcctggtggg cgagggcggc
     3421 cgcgtcctgc caggcgctca gcgcggcggg gaggttgccg tcgagcaagc caccgaagcc
     3481 gaggtagtac atgacgtccg cctcgtgggc ctggtcgccg gccgcgcgga gctcctcgcg
     3541 ggcatcgagc aggatggcga cgccggcttg gcgatcctgc ccaccccgac cggggccgac
     3601 cgcggtatcc agccccgcgc gcaggagcgc ccgcccac
//
LOCUS       DASYGU010000253         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1429708, whole genome shotgun sequence.
ACCESSION   DASYGU010000253 DASYGU010000000
VERSION     DASYGU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429708"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VGO32_07160"
     CDS             <1..1351
                     /locus_tag="VGO32_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7200566.1"
                     /translation="LPRAPVDSVIFVTRSIRAVLVGNFTLRFSTGLTGTLLLLYLADL
                     PDYGGVVVSATTAGLMGALYFVAELILSPVFGVLADRFGTHRLMQPGPIMGAVAVIVT
                     ALTAIAGTAITANVPVLAGTRFLEGAAAAASVPAALGYIAIATSRDQALRGRVIGRFE
                     VATLGGVGIGSVVAGFLYSALGPTAFYVNAAIYGITFLVLRYGVEDLPEHIPPAPGEE
                     TVPASLFHLGRYREVLRSRPIWFLAPTWIALNALLGSWGTQAIFQLVQEPSPEFSDQL
                     LMQGFEPWQAGLVLGVGLLVLFSGLLYWGTRFTRLRRTTIIGLGLAGGLVFVGALLAL
                     NHSTGQAVWFMVATLVAAGIGVFVLAGATPAAVGFLSDISETYPGRRGVIMGLYSVFL
                     ALGQIIGALVAGLAAEWRGMDGLLIASAGLLVVALIPLGQLRASEHHMPGTALDPAP"
     gene            1395..3095
                     /locus_tag="VGO32_07165"
     CDS             1395..3095
                     /locus_tag="VGO32_07165"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV7200567.1"
                     /translation="MRLSRLFFTTLRDDPADAEMISHKLLVRAGYVRQLGSGIYSLLP
                     LGFAVTQRVEQVIREEMNAIGGQEMEMPVVHPAELWKESGRYQKIGPEMVRFKDRGGR
                     DMVLAMTHEEVVADLVRNLVKSYRQLPAIVYHFQTKFRDEPRSRGGLIRVREFVMKDS
                     YTLDADEDGLDHGYQLHYAAYEKIFARLGMKTVVVGADVGIMGGSLAHEFMVLNEHGE
                     DTLVLCESGDYAANQQIAAVMKPEPPTEDPRPTEDVATPAADTIAALAEHLGVATERT
                     AKAIFFVTGDGRMVTAVVRGDYDVNETKLANAVKAVGGMRPAQAEEIKAAGMEPGYAS
                     PIGAHDTVVVVDELAARSPNLVAGANRHGVHLLNVNHGRDFTADLVVDITNAREGDPC
                     PECGKPLVLRQGIEVGNIFKLGTDFTEALGATFLAEDGSRKHVIMGSYGIGLGRNLAC
                     IVEEHHDDKGIIWPREVAPYPAHLVALSASKDPAVAEAAERLYQRLTDAGVNVLYDDR
                     DESPGVKLTDAELLGMPTIVTISPRSLAAGGAEVTDRATGAREVRPLADVEADLQALA
                     "
     gene            3092..5113
                     /locus_tag="VGO32_07170"
     CDS             3092..5113
                     /locus_tag="VGO32_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7200568.1"
                     /translation="MTVDAVEGWSPRQLDTLATLAEAFAPGFAEGDYRRRARQAAETL
                     NAVADPDDLRQLRLVTTALDTPVVNLLIGGRRRRLADLDPAGRDRLLLQWAGSRLAQR
                     RTAFQAFKRLSLFLAWTDTGADGDGNPAWPEIGYVHAEPVAVPSASIAPLAVDRSATE
                     PLALEADVVVVGSGAGGSVVAARLLDAGREVLVVEAGPYLSEADMPVDEGEAFRRLYL
                     DRGTTSTADLGITIVAGAGVGGGTTINWTTCFAPPDWLRDEWASEHGLDGFAGGQTDA
                     DLVRLRAELDIQPPSSVPPKDRAILDGAAALGWEHAVTERNAGPCTDCGACGFGCRAG
                     AKRSALRAHLAAAHAGGARVLADATVEQVTFEGGVTAGVRGWLRSASGEPGRAFTVRA
                     RQVVVAAGALRTPLVLASSGVEHPELGANLRLHPATVVIARMPDPVQMWLGPLQAARS
                     LEFVRPGPATSDGLGPRHGGFFIETGPAHPGLIASAFPWEGGEASRRLMRGARFLIPL
                     GAALRDVSAGRVRWSRRGRPRIDYQLADADRRTAARATVELSRLGRAAGAVELIVGGT
                     PPDRFSAAPGTDVEWAAFLGRRARADFASNRAFFFSAHQMGTARAGADPRSHPCDPHG
                     RVRAGDTTRLIEGLYVADGSLFPTAAGVNPMVTIMALAERTARAILADG"
     gene            complement(5127..5459)
                     /locus_tag="VGO32_07175"
     CDS             complement(5127..5459)
                     /locus_tag="VGO32_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200569.1"
                     /translation="MSFPNTRPIHGVEKGWGLVLAILSFPLGLIALIGLLADQGWARW
                     FGLVLGVLIAITAGVGAILLVLVFLPSQGSSYPFGPWFLFLAAAVAILGLFAARSFLE
                     GLRSTDED"
BASE COUNT          803 a         1971 c         1956 g          838 t
ORIGIN      
        1 cctcccccgc gctcccgtag actcggtcat cttcgtgacc cgctcgattc gcgccgtcct
       61 cgttggcaac ttcacgctgc gcttttcgac cgggctcacg ggcacgctcc tgctgctgta
      121 cctcgccgat ctgcccgatt acggcggggt ggtggtatcg gcgacgacgg ccggcctcat
      181 gggcgccctg tacttcgtgg ccgagctgat cctctcccct gtcttcgggg tgctggccga
      241 ccggttcggc acgcaccggc tcatgcagcc cggcccgatc atgggcgccg tcgccgtgat
      301 cgtgaccgcc ctcaccgcga tcgcgggcac ggcgatcacc gcgaacgtgc cggtcctcgc
      361 cgggacccgc ttccttgagg gcgcggcggc agcggcgagc gttccggccg ccctgggcta
      421 catcgccatc gccacgtcgc gcgaccaggc tctccgggga agggtcatcg gccgtttcga
      481 ggtggccacc ctgggcgggg tgggcattgg ctcggtcgtc gcggggttcc tgtactccgc
      541 cctgggcccc accgccttct atgtcaacgc ggcgatctac ggcatcacgt tcctcgtcct
      601 gcgctacggg gtggaggatc tgcccgagca cattcctccc gcgccgggcg aggagaccgt
      661 ccccgcctcc ctgttccacc tcggacgcta ccgggaggtg ctccgcagcc gcccgatctg
      721 gttcctggcg ccgacgtgga tcgcgctcaa cgcccttctc ggctcgtggg gtacgcaggc
      781 catcttccag ctcgtccagg agccctcgcc cgagttctcg gaccagctgc tgatgcaggg
      841 cttcgagccg tggcaggccg gcctggtgct gggcgtgggg ctgctggtgc tgttctccgg
      901 cctgctgtat tggggcaccc gcttcacccg cctgcggcgg acgaccatca tcggcctggg
      961 attggccggc ggcctcgtgt tcgtgggagc gctcctggct ctcaaccact cgaccgggca
     1021 ggccgtgtgg ttcatggtcg cgaccctggt tgccgcgggc atcggcgtgt tcgtcctggc
     1081 cggtgcgaca ccggcggcgg tcggcttcct gtccgacatc agcgaaacct accccggtcg
     1141 acggggcgtg atcatgggcc tgtactcggt cttcctggcc ctgggccaga tcatcggtgc
     1201 actcgtggct gggctcgcgg ccgagtggcg cggcatggat ggtctgctca tcgccagtgc
     1261 cggactcctg gtcgtggccc tcatcccgct cggccagctg cgcgcctcgg agcaccacat
     1321 gccgggcacc gcgttggacc ccgcccccta gttggcacca gctggccggg ccgcatcggt
     1381 atcctaggcg gccaatgcga ctgagccgcc tgttcttcac gaccctccgc gacgatccgg
     1441 ccgacgccga gatgatttcg cacaagctcc tggtccgcgc cggctacgtc cggcagctgg
     1501 gctcgggcat ctattccctc ctgcccctgg gattcgccgt cacccagcgc gtggagcagg
     1561 tcatccgcga ggagatgaac gcgatcgggg ggcaggagat ggagatgccg gtcgtccatc
     1621 ccgccgagct gtggaaggag tccggccgct accagaagat cggtcccgag atggtccgct
     1681 tcaaggaccg tggagggcgg gacatggtgc tggccatgac ccacgaggag gtcgtggccg
     1741 atctggtccg caacctggtg aagtcgtacc gccagctgcc ggccatcgtc taccacttcc
     1801 agacgaagtt ccgcgacgaa ccgcggtcgc gcggtgggct gatccgggtc cgcgagttcg
     1861 tgatgaagga cagctacacc ctcgacgccg acgaagacgg cctcgaccat ggctaccagc
     1921 ttcactacgc ggcgtacgag aagatctttg cccggctggg catgaagacg gtcgtggtcg
     1981 gcgccgatgt cgggatcatg ggcggcagcc tggcccacga gttcatggtc ctcaacgagc
     2041 acggcgagga cacgctcgtc ctgtgcgaga gcggcgacta cgccgcgaac cagcagatcg
     2101 ccgcggtcat gaagcccgag ccgccgaccg aggaccctag gccgaccgaa gacgtcgcga
     2161 cccccgccgc cgacacgatc gcggcgctgg ccgagcacct gggcgtggcc acggagcgga
     2221 cggcgaaggc catcttcttc gtgaccggcg acgggcggat ggtgacggcc gtggtccgcg
     2281 gtgactacga cgtgaacgag accaagctgg ccaacgcggt gaaggcggtg ggcggcatgc
     2341 ggccggcgca ggccgaggag atcaaggccg ccggcatgga gccgggatac gcctcaccga
     2401 tcggggccca cgacacggtc gtggtcgtgg acgaactggc ggcccgctcg cccaacctgg
     2461 tggccggggc gaaccgccac ggcgtccacc tcctgaacgt gaatcacggc cgcgacttca
     2521 cggcggacct ggtggtcgac atcacgaacg cacgggaagg cgacccctgc ccggagtgcg
     2581 ggaaacctct ggttctgcgc cagggaatcg aggtcggcaa catcttcaag ctgggcacgg
     2641 acttcaccga ggcgctgggt gccacgttcc tggccgagga cggcagccgg aagcacgtga
     2701 tcatgggctc gtacgggatc gggctgggac ggaacctggc ctgtatcgtg gaggagcacc
     2761 acgatgacaa ggggatcatc tggcctcgcg aggtggctcc ctatccggcc cacctggtcg
     2821 ccctgagcgc cagcaaggat cccgccgtgg cggaggcagc ggagaggctc taccagcgcc
     2881 tgaccgatgc cggcgtgaat gtcctgtatg acgaccgcga cgagtccccc ggcgtgaagc
     2941 tgaccgatgc cgagctgctc gggatgccca ccatcgtcac catctcaccg cgctcgttgg
     3001 ccgcgggtgg ggcggaggtc acagaccggg cgaccggcgc gcgcgaggtc cggccactgg
     3061 ccgacgtgga ggccgatctc caggccctcg cgtgacggtc gacgcggtgg agggctggtc
     3121 gcctcgccag ctggacaccc tcgcgacgct ggccgaagcc ttcgcaccgg ggttcgctga
     3181 gggcgactac cgacgccgag ccaggcaggc agccgagacg ctgaatgccg tcgccgatcc
     3241 ggatgacctg cgccagctgc gactggtgac gaccgccctc gacacgccgg tcgtgaatct
     3301 gctcatcggc ggtcggcgcc gccggctggc ggacctcgac cctgcggggc gggatcggct
     3361 cctgctgcag tgggccggca gccgcctcgc gcagcgccgg acagcgttcc aggcgttcaa
     3421 gcgcctgtcc ctgttcctcg cctggaccga taccggcgcc gatggcgatg ggaacccggc
     3481 ctggcccgaa attgggtatg tccacgctga accggttgcc gtcccgagcg cttcgatcgc
     3541 gccgctcgcc gtcgatcgct ccgcgacgga gccgctcgcg ctcgaggcgg acgtcgtcgt
     3601 cgtgggatca ggagccggtg gcagcgtggt cgcggcccgc ctgctggacg cggggcgcga
     3661 ggtgctggtc gtcgaagcgg gtccctacct gtccgaggcg gacatgcccg tcgacgaggg
     3721 cgaggcgttc cggcgcctgt acctcgaccg cggcaccacc tccaccgcgg acctcgggat
     3781 cacgatcgtg gccggggccg gcgtgggcgg cgggacgacg atcaactgga cgacgtgctt
     3841 tgccccgccg gactggctgc gcgacgaatg ggcgtccgag cacggcctgg acggcttcgc
     3901 cggcggacag accgatgccg acctcgtccg cctccgcgca gagctcgaca tccagccacc
     3961 gtcatcggtc ccgcccaaag atcgagcgat cctcgatggc gcggcggccc tcggctggga
     4021 gcacgcggtc accgaacgaa atgcgggtcc gtgcaccgac tgcggggcat gcggcttcgg
     4081 gtgccgcgcc ggagccaagc gatccgcgct tcgcgcgcac ctcgccgccg cccacgcggg
     4141 cggggctcgg gtgctggcgg acgcgaccgt cgagcaggtg acgttcgagg gaggtgtaac
     4201 ggccggggtt cgcggctggc tgcgatcggc gtccggcgaa ccgggacgcg cattcacggt
     4261 ccgcgcccgg caggtggtgg ttgcagccgg agcactccgg acgcccctcg tgctcgcctc
     4321 gagcggagtg gagcaccccg agctaggcgc caacctccga ctccatccgg ccaccgtcgt
     4381 catcgcccgc atgcccgatc cggtacagat gtggctcggc ccactgcagg cggcccgttc
     4441 gctcgagttc gtccgacccg ggcccgcgac gtccgacggg ctgggacccc ggcacggtgg
     4501 ctttttcatc gagaccggcc ccgcccatcc gggactcatc gccagcgcat ttccgtggga
     4561 aggcggcgag gcgagccgac ggctgatgcg cggggcccgc tttctgatcc cgctgggcgc
     4621 cgcgctgcgg gacgtgtccg ccggccgcgt gcggtggagc cgaaggggtc ggccgcggat
     4681 cgactaccag ctggccgacg ccgacaggcg gacggcggcc cgcgccacgg tggagctcag
     4741 ccgactcggg cgggccgccg gcgccgtcga gctcatcgtc ggcggcacgc cgcccgaccg
     4801 gttcagcgcc gcgccgggta cggacgtgga gtgggccgcc ttcctgggcc ggcgcgcgcg
     4861 agccgacttc gcctcgaacc gggcgttctt cttctcggcc caccagatgg gcacggcgcg
     4921 cgccggcgcc gatccacgga gccacccgtg cgacccgcac ggtcgcgtcc gcgcagggga
     4981 caccacgcgc ctcatcgagg gtctctacgt ggccgacggt tcgctcttcc cgaccgccgc
     5041 cggggtgaac ccgatggtca cgatcatggc tctcgccgag cgcacggcgc gggcgatcct
     5101 cgccgacggt tgagaggctc gaccggctag tcctcgtcgg tggagcggag accctcgagg
     5161 aacgaccgag cggcgaacag cccgagaatg gcgacagccg cggccaggaa caagaaccac
     5221 gggccgaatg ggtagctcga tccctgcgac ggcaggaata ccagcaccaa caggatggcc
     5281 ccgacccccg ctgtgatggc gatgagcacg ccgaggacga gcccgaacca gcgcgcccat
     5341 ccctggtcgg cgagcaggcc gatcagcgcg atgaggccca gcgggaacga caggattgcg
     5401 agcacgaggc cccaaccctt ctccacgccg tggatcggcc gagtatttgg aaagctcatc
     5461 acgcgacctc ccgacgcatc ctaggcgccc cgcagctggg gaaacacgtt cgcggccagc
     5521 cattccaggc ggctcgtgtc gttcaccccg cgcacgctga agatgacg
//
LOCUS       DASYGU010000254         9567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_247198, whole genome shotgun sequence.
ACCESSION   DASYGU010000254 DASYGU010000000
VERSION     DASYGU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247198"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VGO32_07180"
     CDS             complement(<1..1878)
                     /locus_tag="VGO32_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7200570.1"
                     /translation="MDARANVLGAEAPAFDTDEAVAMARALFGVAGTADRLVSERDQN
                     FRLTLADGSRWVLKISNEVEDAAVVDMEVEAVRHVARMDPALPVAVARLAESGQAVVQ
                     VPGRSGGQHQVRLIPFLPGQHVEPSSLSPTALAEIGGTTARLARAMRGFFHPAAGRTI
                     LWDLKHLDALRPHLDAIAEPDRRALVERALERFDRHVAPALPTLRSQVIHNDLTLDNL
                     LMDGDRVAGILDFGDMAHTALVLDLPAMLQSVLRGRSDPFEAAASAIGGYVQVTPLEA
                     EEADLLADMLAARMVQTVLISVWRTADYPDNAYIRGWLEPAYELLEWLEGAGWDVARR
                     RLADAAGRPAAVPAIASAVLAARRATVLGSALSPLTYDRPLHLVRGDGVWLYEADGTR
                     YLDAYNNVPVVGHAHPVVVDAIARQAGMLNSNTRYLHEAVVVLAERLVASMPPGLDTV
                     MFVNSGSEANDLAWRLATTFAGGTGGIVSQWSYHGLTAAVADLSTSEWPRGEQPATVE
                     TIPAPNPYRGPHRDDPDPDAASAAELAAAVARLAERGVPAAALYLDGMFTADGIVPPS
                     RRYLELIAGAARAAGVVFVADEVQSGHGRSGEGLWSFGAAGVTPDIVTLGKPMGNGHP
                     IA"
     gene            1903..2676
                     /locus_tag="VGO32_07185"
     CDS             1903..2676
                     /locus_tag="VGO32_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7200571.1"
                     /translation="MRDDEPNYIQRNRAAWDGWAAEYAEAGERAWARTEPTWGIWGVS
                     EADVHLLPDDLHGKDVIELGCGTAYVSAWLARRGARVTGIDNSPAQLETARRLQTEHG
                     LDFPLLLGYAEQVPLPDASFDLAISEYGAAIWADPYAWIPEAARLLRPGGDLIFLGNS
                     VLVMLSVPDLEADGPATDRLLRPQFEMHRFEWPDEDSVEFHISHGDMIRLLRDSGFEV
                     LDLVEIRPPLDATTNYPNIATIEWSRRWPSEEVWKARKR"
     gene            2686..3282
                     /locus_tag="VGO32_07190"
     CDS             2686..3282
                     /locus_tag="VGO32_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200572.1"
                     /translation="MGWDSGSLGPPLSDVQVELYTSGYQVSGHMMTRFHKVADILNLG
                     GSTHLAIERATVAEYADPGAARGGQSVLVAVEAVLFGISSGVDDAPAADIVVQKRPVK
                     IQVAFPPFWLIGTFHVPQGSEAMDVLNVADPFLSLTDAAVTSAAFPGFDGDAPIVAFQ
                     RKLAQVLIVTDDVAHGSGLEDIIPEDEARSWLPPADPA"
     gene            complement(3284..3835)
                     /locus_tag="VGO32_07195"
     CDS             complement(3284..3835)
                     /locus_tag="VGO32_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200573.1"
                     /translation="MTPSTGGRGLARLRSSDEDRAARNDARALEGLLKPVLDDSYVLI
                     LSPQIPGVDGALRGLLVGPGGVRALLVRRWNGHFRQRGRMWEYNARGRLGWIPCRTDP
                     TRDGRLVLDQLSEWMRDALGTVLPLEATIAFPDRTSHVELTNDPEIEVVTRDNAPWWA
                     NSLAKARRMDERSAGRLLVALGA"
     gene            3978..4871
                     /locus_tag="VGO32_07200"
     CDS             3978..4871
                     /locus_tag="VGO32_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV7200574.1"
                     /translation="MWRGAIQFGLVTIPVKLYLATESTGIGFNMLHASCLNRIQMKIY
                     CPFHDEVVPRSETVRGYEWSKGRYVVVTDDDLEAVPLKTVRSIEIAMFVDAGKEAHGA
                     QFVKSAYYLEPDPVGKKAFFLLRQVLADEGKTALCKVVMKDREQLAAINPYSESMLLT
                     TLHWPDEIRSVEELAVDSSGIEIKPSERKMAEQLIASMTGEFDAEEYRDEYRQALMGV
                     IESKVAGEEPTPIKATEPTKIGDLMAALEASVAAAREARRDAAAGDAEPEAKPTPARR
                     SRAKKSAEADQPPQTRRRKTA"
     gene            4880..5581
                     /locus_tag="VGO32_07205"
     CDS             4880..5581
                     /locus_tag="VGO32_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200575.1"
                     /translation="MPAEQLPLPTTPGESPRLRLPDRISPMQPGEVDAPFDDQDYLFE
                     PWWPGVRAIAFSERGRVRLQVAGLADGDAAFPELADLPGQLAEDGVAVEGTLLVLDGE
                     GRPDGDLLRARLSGGEPRAGRAAYVAADLLWAGGEPVTKRTFRVRRDWLTTLLAPGDR
                     ITVGHGYVGDGTLVAEALARLGIDGLSARRLSTRYRAGAAGDAWLRMPVTPAEPRPRP
                     TLALLLRLPLDEPQS"
     gene            complement(5572..6642)
                     /locus_tag="VGO32_07210"
     CDS             complement(5572..6642)
                     /locus_tag="VGO32_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HEV7200576.1"
                     /translation="MNDRLTEPDPLDEEPYDGAAFDDEPAEGPTGSGRKRDRLARLLS
                     VITILYTRGSGDQGVAVADIARLTGMNVRTVYRDLKALEDELAVPIFQAGRGRYGIER
                     KYFLPPLQLTVPEGIVLFLASRLIAQWSDESDQAVISAFTKIASELPQPIARHVTATM
                     LAVGEQDSNEPFTAIFNSVARGWAEGRVVEITYEPGSGEPRQTRVRPYLLEPFAAGRS
                     IYLIGHDETAGAMRTYKIERIRQAILTLDRYEIPDDFDPDQFLANSWGIWSSDTTPPV
                     DVRLRFDAGVARRVRETIWHRSQSLADLEDGGVELTVRVAGIVEIRPWVLSWGDQVEV
                     LEPAELRDAVATAARGLTARYD"
     gene            complement(6653..7393)
                     /locus_tag="VGO32_07215"
     CDS             complement(6653..7393)
                     /locus_tag="VGO32_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200577.1"
                     /translation="MHDRRRAPLRPAPRTVLRGRGLWTAAIVMIAAVACATSPSPTTS
                     PSASLGPTPSPTPTPQPTPRFSNEADPALSALIPDEVTGLVVVKPAITDYAITPGDIG
                     QTAYGELGARFQALVIAFIREPRLSLYAMRVDGPITTEELEPYLATAGRYVGISGLDP
                     EPWELTEVGGHWVWVRPSDNATLPGTQVYTWAVDDVVFLMIGVSSDLNQALLALLPGE
                     PPPTPSPAPSTSPGASEAPDPSASPSGS"
     gene            complement(7440..>9567)
                     /locus_tag="VGO32_07220"
     CDS             complement(7440..>9567)
                     /locus_tag="VGO32_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7200578.1"
                     /translation="ELTGQLELGAVLSDIADRTRGLFDADRVGLWMINDEREHPFDLA
                     AERGISPAFQATIAQLTLNSPSLGTLAVRERRTFVARHVDTDPAVGILSDIYRDEGVK
                     TACLVPLVSHDRAVGVIGLYHSRDREWPDDELALAQAFANQAAVAISNARLYRSTAEQ
                     AARMRSIHDLSARLNRLTDVQAIADAIVAEASTLAAFHDIRIYTVDWEHGTCEPIAFT
                     DRLLGDGDFRDLLRVDIGPGSFTGWVAANAQPILSNDALNDQRGHTIDGTEEIEESML
                     VVPMVYEGRSVGVIALSKLGVNQFSTDDQQTMMIFAGYAAQALVNARSYRRLEEQSSQ
                     LARQLDSQRRLLDVSTRVMGTLEQEDILEVVADGLKAVVHYDNLSIYRADGVKQVLVP
                     VLTREEHAEEVARYIIPFGRGLMGWVVQHAEAVLANDALADPRALQIPGTPNDQEALI
                     VVPLISDGEVIGSMNVSRVGGEDVYFSETDFELVQLFAAQAAVALRNADEHHAMSMRA
                     ETDALTGLGNHGAFQRDLTEATDAAVNAPPEAGQLALLMMDLDRFKTYNDRFGHPAGD
                     ALLHAVGEALYAATRSDDHVYRYGGDEFTVVLSGVDLAAAQRVAERARRMVSAVSLER
                     GAAPVTITIGVALFPDHGATKDALIAAADAALYAGKQVGGDRVVVPGEAAKLADAKPP
                     KPRPAAAGVEQRRRRSDTRRTKTTAA"
BASE COUNT         1412 a         3349 c         3392 g         1414 t
ORIGIN      
        1 cgcgatcggg tggccattgc ccatcggctt gcccagcgtg acgatgtcgg gcgtcacgcc
       61 ggccgcgccg aagctccaga gaccctcccc cgaccgcccg tgccccgact gcacctcgtc
      121 ggccacgaac accaccccgg cggcccgcgc cgctcccgcg atcagctcga ggtagcggcg
      181 gctgggcggc acgatgccgt ccgcggtgaa catgccgtcc aggtagagcg cggccgcagg
      241 cacgccgcgc tccgcgagcc gtgcgaccgc ggcggccagc tccgcggcgg aggccgcgtc
      301 cgggtcgggg tcgtcgcgat gggggccgcg gtacgggttg ggcgccggga tggtttcgac
      361 cgtggccggc tgctcgccgc gcggccattc ggaggtggac aggtcggcga cggccgcggt
      421 cagtccgtgg tacgaccact ggctgacgat gccgccggtc ccgccggcga acgtcgtcgc
      481 gaggcgccag gcaaggtcgt tcgcttcgct gcccgagttc acgaacatga cggtgtcgag
      541 gcccggcggc atgctggcca cgagccgctc ggccaagacg accacggcct cgtgcaggta
      601 ccgcgtgttc gagttgagca tcccggcctg gcgggcgatg gcatccacca ccaccgggtg
      661 cgcatggccg acgaccggga cgttgttgta cgcatcgagg tatcgcgtgc catcggcctc
      721 gtacaaccag acgccgtcac cgcggacgag gtggagcggc cggtcatagg tcaacggcga
      781 gagcgcggag cccaggactg tggcgcgtcg ggccgccagc accgcgctcg cgatggccgg
      841 gaccgccgcc ggtcggcccg ccgcatcggc caggcgtcgc ctggccacgt cccaccccgc
      901 cccctcgagc cattcgagca gctcgtacgc cggctccagc cagccgcgga tgtatgcgtt
      961 gtcggggtag tccgcggttc gccataccga gatgaggacg gtctggacca tgcgggcggc
     1021 gagcatgtcg gcgagcagat cggcctcctc ggcctcgagt ggcgtcacct ggacatagcc
     1081 gccgatggct gatgcggccg cctcgaacgg gtcgctccgg ccccggagca cggactggag
     1141 cattgccggc aggtcgagga cgagcgcggt gtgggccatg tccccgaaat cgagaatgcc
     1201 cgccacccgg tcaccgtcca tcagcaggtt gtccagggtc aggtcgttgt ggatgacctg
     1261 cgaccggagc gttggcaggg cgggcgccac atgccggtcg aatcgctcca gcgcccgctc
     1321 cacgagtgcc cgccggtcgg gctccgcgat ggcgtcgaga tgcgggcgca gggcgtcgag
     1381 atgcttgagg tcccagagga tggtccggcc cgcggccggg tggaagaagc cccgcatcgc
     1441 ccgtgccagg cgggccgtgg tccccccgat ctcggccagc gcggtggggc tcaggctgct
     1501 cggctcgaca tgctgtcccg gcaggaacgg gatcagccgg acctggtgct ggccacccga
     1561 gcggcccgga acctggacca ccgcctgccc gctctcggcc agtcgggcca ccgccaccgg
     1621 cagcgccggg tccatgcgcg cgacatgccg gacggcttcg acctccatgt ccaccacggc
     1681 ggcgtcctcg acctcgttcg agatcttcag cacccaccgg ctgccatcag ccaacgtcag
     1741 gcggaagttc tggtcgcgct cgctcacaag acggtcggcg gtcccggcga cgccgaagag
     1801 ggcgcgggcc atggccaccg cctcgtccgt atcgaaggcc ggtgcctccg cgccgaggac
     1861 attcgctcgc gcatccacga gccgatcgta cacactggcc cgatgcgcga cgacgagccg
     1921 aactacatcc agcgcaaccg cgccgcgtgg gacggctggg ccgcggagta cgccgaggcg
     1981 ggcgagcggg catgggcccg gaccgagccg acctggggca tctggggcgt atcggaggcc
     2041 gatgtccacc tcctgcccga cgatctccac ggcaaggacg tcatcgagct gggctgcggg
     2101 acggcgtacg tctcggcctg gctcgcccgc cgcggcgcca gggtgaccgg gatcgacaac
     2161 tcaccggccc agctggagac cgcgcggcgg ctacagacgg agcacggcct cgactttccc
     2221 ctcctgctcg gctatgccga gcaggttccg cttcccgacg cgagcttcga cctggccatc
     2281 tcggagtacg gcgcggccat ctgggccgat ccctacgcct ggatcccgga ggccgcgcgc
     2341 ctcctccgtc cgggtggcga cctcatcttc ctcggcaaca gcgtgctggt catgctctcc
     2401 gtgccggacc tggaggcgga cgggccggca actgatcggc tcctgcgccc gcagttcgag
     2461 atgcaccgct tcgaatggcc cgacgaggac agcgtcgaat tccacatctc gcatggcgac
     2521 atgatccggc tcctgcgcga cagcgggttc gaggtcctcg acctggtcga aatccggccc
     2581 ccgctcgacg caaccaccaa ctacccgaac atcgcgacca tcgaatggtc gcgccgctgg
     2641 ccgagcgagg aggtctggaa ggcgcgcaag cgctaggatc gcggcatggg gtgggacagc
     2701 ggatccctgg ggccaccgct cagcgacgtg caggtcgagc tgtacacgtc gggctaccag
     2761 gtgagcggac acatgatgac ccggtttcac aaggtcgccg acatcctcaa cctgggcggc
     2821 tccacgcacc tcgcgatcga gcgggcgacc gtggccgagt acgccgatcc gggtgccgcg
     2881 cgcgggggcc agtcggtcct ggtggccgtc gaggcggtcc tgttcgggat cagctcaggc
     2941 gtggacgacg ccccggccgc ggacatcgtg gtccagaagc gaccggtcaa gatccaggtc
     3001 gccttccctc cgttctggct catcggcacg ttccacgtgc cacagggcag cgaggccatg
     3061 gacgtcctga acgtcgcgga tccattcctt tccctgaccg atgcggcggt gacctcggcc
     3121 gcatttcccg gcttcgacgg cgacgccccg atcgtcgcct tccagcgcaa gctggcccag
     3181 gtcctgatcg tgaccgatga cgtggcccac ggatcgggcc tcgaggacat cattccggag
     3241 gacgaggcgc gcagctggct gccgcccgcc gatcccgcct aggtcacgcc cccagcgcca
     3301 ccagcaggcg gccggcactt cgttcgtcca tccgtcgggc cttggccagg ctgttcgccc
     3361 accagggtgc attgtcccgg gtcacgacct cgatctcggg gtcgttcgtg agctcgacgt
     3421 ggcttgtccg atcggggaat gcgatcgtgg cctccagcgg aagcacggtc cccagggcat
     3481 cgcgcatcca ctcgctgagc tgatcgagga cgaggcgccc gtcgcgtgtc ggatccgttc
     3541 gacacgggat ccagcccagg cgtccgcgcg cgttgtactc ccacatccgc ccgcgctgcc
     3601 gaaagtggcc gttccagcgc cggacgagga gcgcccgcac tccccccggt ccgacgagga
     3661 gaccccggag tgctccgtcc acgcccggga tctgcggcga gagaatgagg acgtaggagt
     3721 cgtcgagcac cggcttgagc agcccttcga gcgcacgcgc gtcgttcctg gcggcacggt
     3781 cctcgtcgct cgacctgagt ctggccagac cacggccacc ggtcgacggt gtcatcacct
     3841 gcacgtcgtt caggatcggc cagagagccg ccggcccgca atccggcgga acgggcaccc
     3901 cacaagtact gggcacggac ccacttgcgg gagccagcgc gaccgggtgt atcctcacca
     3961 accctatggc acgagcgatg tggcggggcg ccatccagtt cggtctggtg acgatcccgg
     4021 tcaagctcta cctcgcgacc gagtcgaccg gcatcggctt caacatgctg catgccagct
     4081 gcctgaaccg gatccagatg aagatctact gcccgttcca cgacgaggtg gttccgcgct
     4141 cggagaccgt ccgcggctac gagtggagca agggccggta cgtggtcgtg accgacgacg
     4201 acctcgaggc cgtgccgctc aagaccgttc gctctatcga gatcgcgatg ttcgtggatg
     4261 ccggcaaaga ggcccacggc gcgcagttcg tgaagagcgc ctactacctc gagcccgatc
     4321 ccgtcggcaa gaaggcgttc ttcctgctcc gccaggttct ggccgatgaa ggcaagaccg
     4381 ctctctgcaa ggtggtcatg aaggaccgcg agcagctcgc ggccatcaac ccgtactccg
     4441 agtcgatgct cctcaccacc ctccattggc ctgacgagat ccggtcggtc gaggagctgg
     4501 cggtggacag cagcgggatc gagatcaagc cgtcggagcg gaagatggcc gagcagctca
     4561 ttgcctcgat gaccggcgag ttcgacgccg aggagtaccg cgacgaatac cggcaggcgc
     4621 tgatgggcgt catcgagtcc aaggtcgccg gcgaggagcc gacgccgatc aaggccaccg
     4681 agccgaccaa gatcggcgac ctgatggccg ccctggaggc cagcgtcgcg gccgcccgcg
     4741 aagcgcgccg cgatgccgcg gccggggatg ccgagcccga agccaagccg acaccggctc
     4801 gccgaagccg cgcgaagaag tcggccgagg ctgaccagcc tccccagacc cggcggcgca
     4861 agaccgcctg agcggccgca tgccggccga acagctcccg ctgcccacca cgcccggcga
     4921 gtctccgcgt ctgcggttgc ccgatcggat cagccccatg cagccgggcg aggtcgatgc
     4981 accgttcgac gatcaggact acctgttcga gccgtggtgg cccggtgttc gcgcgatcgc
     5041 attctccgag cgcgggcggg tccggttgca ggtggccggg ctggcagacg gcgacgccgc
     5101 gttccccgag ctggcggacc tcccgggcca gctggccgag gacggcgtgg cggtcgaggg
     5161 gacgctgttg gtcctcgatg gcgagggccg tcccgatggc gacctcctcc gggcgcgcct
     5221 gagcggcggg gagccgagag ccggccgagc ggcgtatgtc gcggccgacc ttctgtgggc
     5281 gggcggcgag ccggtcacga agcggacgtt ccgggtgcgt cgggactggc tgaccacgct
     5341 cctcgcgccc ggagaccgga tcacggtcgg ccacggctac gtgggggatg ggacactggt
     5401 ggccgaggcg ctggcgcgac tcgggatcga cggcctctcg gcgcgtcgac tgtcaacgcg
     5461 ctaccgcgcc ggagcggcgg gcgatgcatg gctgcgaatg ccggttacgc cggccgagcc
     5521 gcggccccgg ccgaccctcg ccctcctcct ccgcctcccg ctggacgagc ctcagtcgta
     5581 gcgggcggtc aacccgcgcg cggcagtcgc caccgcgtca cggagctcgg ccggctccag
     5641 cacctccacc tgatcgcccc aggacaggac ccagggccgg atctcgacga ttcccgccac
     5701 ccgcaccgtg agctcgacgc cgccgtcctc caggtcggcc aggctctgcg accggtgcca
     5761 gatcgtctcc cggacccggc gcgcgacccc cgcatcgaac cgcaggcgca cgtcgaccgg
     5821 tggcgtcgtg tcggacgacc agatgcccca cgaattggcg aggaactggt ccgggtcgaa
     5881 gtcgtcggga atctcgtatc ggtccagggt caggatcgcc tgtcggatgc gctcgatctt
     5941 gtaggtccgc atcgcgcccg ccgtttcgtc gtggccgatg aggtagatgc tccgaccggc
     6001 ggcgaacggc tcgaggaggt acgggcggac acgcgtctgg cgtggctcgc cgctcccggg
     6061 ctcgtacgtg atctcgacca cccggccttc cgcccagcca cgggcgacac tgttgaagat
     6121 cgccgtgaac ggctcgttcg agtcctgctc cccgaccgca agcatcgtgg cggtcacgtg
     6181 gcgggcgatg ggctgcggaa gctcggacgc gatcttggtg aaggcgctga tgactgcctg
     6241 atccgactcg tccgaccact gcgcgatgag acgggaggcc aggaagagga cgatgccttc
     6301 cggcacggtc agctggagcg gcggcaggaa gtacttccgc tcgatcccgt accggccgcg
     6361 cccggcctga aagatgggca ccgccagctc gtcctccagc gccttcaggt cgcggtagac
     6421 ggtccggacg ttcatgccgg tcaggcgcgc gatgtcggcc acggccacgc cctggtcgcc
     6481 gctgccccgg gtgtacagga tcgtgatcac cgacagcagc cgcgccaggc ggtcgcgctt
     6541 gcgccccgag ccggtcggtc cctcggccgg ctcgtcatcg aacgccgcgc cgtcgtacgg
     6601 ctcctcgtcc agcgggtccg gctcggtcag cctgtcgttc accgccgaca gtctaggagc
     6661 cgctcgggct cgcggacggg tccggcgcct cgctcgcccc ggggctggtg gacggcgccg
     6721 ggctgggggt cggcggcggc tcgcccggga gcagggccag gagcgcctgg ttaaggtcgc
     6781 tgctgacccc gatcatcagg aacacgacgt cgtccacggc ccaggtatag acctgcgtgc
     6841 cgggaagggt cgcgttgtcg gacgggcgaa cccataccca gtggccgccc acctccgtca
     6901 gctcccatgg ctcggggtcg aggcccgaga tgcccacgta ccgaccggcc gtggccaggt
     6961 agggctccag ctcctcggtg gtgatcggcc cgtcgacccg catcgcatac aggctcagcc
     7021 gcggctcccg gatgaacgcg atgacgaggg cctggaaccg ggcgcccagc tccccatagg
     7081 cggtctggcc gatgtcgccg ggcgtgatcg cgtagtcggt gatcgccggc ttcacgacca
     7141 cgaggccggt cacctcgtcc gggatgagcg cggacagggc gggatcggcc tcgttcgaga
     7201 agcgaggggt cggctgcggc gtgggggtgg gcgacggggt cggaccgaga gaggcgctcg
     7261 gcgacgtggt cggcgatggt gaggtggcgc aggccacggc cgcgatcatc acgatggcag
     7321 ccgtccacag accgcgcccg cggaggacgg tccgcggcgc agggcgcagc ggagcgcggc
     7381 gtcggtcgtg catttggggg gccgaggata acccgatccg cggcggaccg ggcgcgaggt
     7441 caggctgcgg tggtcttcgt tcggcgggtg tcgcttcgac gacgtcgctg ctcgacgccg
     7501 gccgccgccg gtcgcggctt cggcggcttc gcgtccgcga gtttggcggc ttctccgggg
     7561 acgactaccc gatcgccgcc aacctgcttg ccggcataca gcgcggcatc ggccgccgcg
     7621 atgagggcgt ccttggttgc gccatggtcg gggaagaggg cgacgccgat cgtgatcgtg
     7681 accggggccg cgccgcgctc gagcgagacg gcagacacca tccgccgcgc gcgctcggct
     7741 acacgctggg cagcagccag atcgacgccc gacaggacga cggtgaactc gtcgccaccg
     7801 tagcggtaca cgtggtcgtc gctgcgggtc gcggcgtaca gggcctcgcc aaccgcgtgg
     7861 aggagcgcgt caccggccgg gtggccgaat cggtcgttgt aggtcttgaa gcggtcgagg
     7921 tccatcatca gcagggccag ctgcccggcc tccggcgggg cgttcaccgc cgcatcggtg
     7981 gcctcggtca ggtcgcgctg gaaggctccg tggttgccca gcccggtcag ggcatccgtt
     8041 tcggcccgca tgctcatggc gtggtgctca tcggcgttgc gcagggcgac ggccgcctgg
     8101 gcggcgaaca gctggacgag ctcgaaatcg gtctcgctga agtagacgtc ctcgccaccc
     8161 acccggctca cgttcatcga gccgatcacc tcgccgtcgc tgatgagcgg gacgacgatg
     8221 agcgcctctt ggtcattcgg agtaccgggg atctggagcg cccgcggatc ggccagggcg
     8281 tcattggcca ggaccgcctc ggcgtgctgg accacccagc ccatcaggcc gcggccgaac
     8341 gggatgatgt agcgcgcgac ctcctccgca tgctcctcgc gggtcagcac cgggacgagg
     8401 acctgcttca cgccgtcggc gcggtagatg gacaggttgt cgtagtggac caccgccttc
     8461 aggccgtcgg cgacgacctc gaggatgtcc tcctgctcga gggtgcccat gacccgggtg
     8521 ctgacgtcca gcagccgtcg ctgcgagtcg agctggcggg cgagctggct ggactgctcc
     8581 tccaggcgcc gatagctgcg cgcgttgacc agggcctggg ccgcatagcc ggcgaagatc
     8641 atcatcgtct gctggtcatc ggtcgagaac tggttgacgc ccagcttgga cagggcgatc
     8701 acgcccactg atcggccttc gtagaccatg ggcacgacta gcatcgactc ctcgatctcc
     8761 tcggtgccgt cgatcgtgtg cccgcgctgg tcgttcagcg cgtcattgct gaggatgggc
     8821 tgcgcgttgg ccgccaccca gccggtgaac gagccggggc cgatgtccac ccggagcagg
     8881 tcgcggaagt caccgtcgcc cagcagccgg tcggtgaacg cgatcggctc gcaggtgccg
     8941 tgctcccagt ccacggtgta gatccggatg tcgtggaacg cggccagggt gctcgcctcg
     9001 gccacgatcg cgtcggcgat ggcctggacg tccgtcagcc ggttcaatcg ggccgacagg
     9061 tcgtgaatgg accgcatgcg cgccgcctgc tcggcggtcg accggtacag gcgggcgttg
     9121 ctgatcgcga ccgccgcctg gttggcgaat gcctgggcca gggccagctc gtcatccggc
     9181 cactcccgat cgcgggagtg gtacaggccg atcaccccga ccgcgcggtc atggctcacg
     9241 agaggcacga ggcacgcggt cttgacgccc tcgtcgcggt agatgtcgga caggatgccg
     9301 acagccggat cggtgtcgac gtggcgggcc acgaaggtcc ggcgctcgcg aacggccagc
     9361 gtcccgagcg aggggctgtt gagggtcagc tgcgcgatgg tggcctggaa ggcagggctg
     9421 atcccgcgct ccgcggcgag gtcgaatgga tgctcccgtt cgtcgttgat catccacagg
     9481 cccacccggt ccgcgtcgaa caggccacgg gtccgatccg cgatgtcgct gagcacggcg
     9541 cccagctcga gctggcctgt gagctcc
//
LOCUS       DASYGU010000255         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_813298, whole genome shotgun sequence.
ACCESSION   DASYGU010000255 DASYGU010000000
VERSION     DASYGU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813298"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VGO32_07225"
     CDS             <1..555
                     /locus_tag="VGO32_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200579.1"
                     /translation="QLDNVEDLLADVTASPRFDADVFTLERSLQVMLGNRAGSTLIGL
                     LTVTREVFEEVGQIGLAPEIIERIVGWRARFGLAIDNALNFLNNPDGIQGHNLGTQVI
                     HGEGGSTLQGRLTLVRYNNEPQFFIADADILLHIAGDLMEQLGEHLEPDMIDAELVAR
                     LREAVERIERRAGPPPAASPLAED"
     gene            559..1140
                     /locus_tag="VGO32_07230"
     CDS             559..1140
                     /locus_tag="VGO32_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEV7200580.1"
                     /translation="MATSTAPVLPFTDNPDANVLLGADPLALLIGYALDQQVTVQHAF
                     TGPWELRRRIGHLDPFRIASMDPGELEHVFRERPALHRYPASMATRVADLCRIVADTY
                     GGDASRIWTEAHDGADLEKRLLALPSIGQMKVYGLLAILFKRFGVRLPGLEERLPTWP
                     TLGDVDTPEALEHYQTQKRAYKAALRAEAATKA"
     gene            complement(1237..2049)
                     /locus_tag="VGO32_07235"
     CDS             complement(1237..2049)
                     /locus_tag="VGO32_07235"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV7200581.1"
                     /translation="MEVVRGVDELRDAILELRRAGHSIGFVPTTGLVHSGQLSLVERA
                     RETDGAVVVSNQPDPGGTATEEERLLVLLGQTGVDLVFLPGHGTVDPPHVTRITVDGL
                     TGRLEGASRPGYLDEVATTTMKLLHLVGPARLYLGQKDGQRVVVVRRMLADLFANVEL
                     VVCPVLRDPDGLAVSAANRGLSIEERHAATCLFAAIAAAEAAYDGGERSAEVLRARMT
                     ERISAESLARIDYLSVADDESLAELSRLDRPALAMAAVWIGRTRLTDSVLLG"
     gene            complement(2052..3359)
                     /gene="coaBC"
                     /locus_tag="VGO32_07240"
     CDS             complement(2052..3359)
                     /gene="coaBC"
                     /locus_tag="VGO32_07240"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV7200582.1"
                     /translation="MTEGTDGTLVGRRVVVGVSGSIAAYKAAVLVRQLMNAGAIVDVA
                     MTPAATAFVTPLTFASLTHRPVITDVLALDPDQHIAHIELAEAADAIVLAPATANLLG
                     ELAAGLVDDAVTAIVAASRARVVVAPAMDAGMWTHPATQRNVETIRGFGYLVVEPEVG
                     ALASGLTGIGRLAEPEAVVAAVERLFARAGDLDGLRVAISAGGTREPIDPVRFIGNRS
                     SGKMGVALAEAARDRGAAVTLVAGAMSVTAPRGVNVVDATTASDMREAVLRIAPSADI
                     LVMAAAVADYAPAKTSGQKIKRGSESLRLELVPNPDIIAEVGEMPEAIRPFLIGFAAE
                     SDDLEANATGKLRDKGLDLIVANKVGGPFDAIGSEENKVVVFGREGTLTDWPMLPKRQ
                     VAERLWDLIADRYRARGDTPSGPARASRSTRSRSPRRARQQDT"
     gene            complement(3356..>4070)
                     /locus_tag="VGO32_07245"
     CDS             complement(3356..>4070)
                     /locus_tag="VGO32_07245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV7200583.1"
                     /translation="RATDDELAATVTGLLALDGHRLDDVDAAALACVVPPLVARWTRM
                     LARRARVRPFIVDAAALDGILAIEIDRAAEAGADRLANALAAREEFDGPAIVIDMGTS
                     TNFDVLSAEGAYIGGAIAPGLGISVDALVGRTSKLPRIELVRPSQAIGSNTIAAMQSG
                     TVNGYIGLLSGLLAAIRGELVERSPRDARVTVIATGGFALQPWLADVPGIDRVEPELT
                     LRGVRYASALAAARTESRT"
BASE COUNT          609 a         1471 c         1420 g          570 t
ORIGIN      
        1 cagctcgaca acgtcgagga cctcctggcc gatgtgacgg ccagcccccg cttcgacgcc
       61 gacgtcttca ccctcgagcg gtcgctgcag gtcatgctcg gcaaccgggc gggctccacc
      121 ctcatcggcc tgctgaccgt cacccgcgag gtgttcgagg aggtgggcca aatcgggctg
      181 gcgccggaga tcatcgaacg catcgtcggc tggcgggccc ggttcgggct ggccatcgat
      241 aacgctctga acttcctgaa taacccggac gggatccagg gccacaacct gggcacccag
      301 gtcatccacg gcgagggtgg ctcgaccctc cagggccggc tgaccctcgt ccgctacaac
      361 aacgagccgc agttcttcat tgccgacgcg gacatcctgc ttcacatcgc gggcgacctc
      421 atggagcagc tcggtgagca cctggagccg gacatgatcg acgctgagct cgtcgctcga
      481 cttcgcgagg cggtcgagcg catcgagcgc cgggccggcc cgccgcccgc cgcgagcccg
      541 ctcgccgagg actgaccgat ggccaccagc accgcgcccg tcctgccctt caccgacaac
      601 ccggatgcca atgtcctcct cggggcagac ccgctggccc tcctcatcgg ctacgcgctc
      661 gaccagcagg tcaccgtcca gcacgcattc accgggccgt gggagctccg ccgccgcatc
      721 ggccacctcg acccgttccg gatcgcatcc atggacccgg gcgagctgga gcacgtgttt
      781 cgcgaacgcc cggcgcttca tcgctatccg gcgtcgatgg ccacccgggt ggccgacctg
      841 tgccggatcg tggccgacac gtacggcggt gacgcgagcc ggatctggac cgaggcgcac
      901 gatggcgcag acctcgagaa gcggctcctc gccctgccgt ccatcggcca gatgaaggtc
      961 tatggtctgc tcgcgattct gttcaagcgg ttcggcgttc ggttgcccgg gctcgaggag
     1021 cggctgccca cctggccgac cctgggcgac gtcgatactc ctgaggcgct cgagcactac
     1081 cagacccaga aacgcgccta caaagcggcc ctccgcgcgg aagcggcgac gaaggcctag
     1141 gggttcaaaa aggcaacgaa aaggaggccg gacgtgggca ctcgccggcc tccccagccc
     1201 atcctaccca tccgcgatgc ggggtcagta gtactttcag cccagtagga cactgtccgt
     1261 gaggcgggtc cgaccgatcc acaccgcggc catcgccagg gccggacgat ccagccgtga
     1321 aagctcggcg agcgactcgt catcggccac cgacaggtag tcgatccgcg ccagtgattc
     1381 ggcactgatc cgctcggtca tccgggcgcg gagcacctcg gccgaccgct ccccgccgtc
     1441 gtacgcggcc tccgcggccg cgatcgccgc gaacaggcag gtcgccgcgt ggcgctcctc
     1501 gatcgacagc ccgcggttcg ccgccgagac ggcaaggccg tccggatcac ggaggaccgg
     1561 gcacaccacc agctcgacgt tggcgaacag gtccgccagc atccgccgca cgacgaccac
     1621 ccgctggccg tccttctgtc ccaggtagag gcgggccggc ccgacaaggt ggagcaactt
     1681 catggtcgtg gtcgcgacct cgtccaggta ccccggccgc gatgcgccct cgagcctgcc
     1741 ggtcaggccg tccaccgtga tccgggtcac atggggagga tccacggtcc cgtggccggg
     1801 caggaacacg aggtccacgc cggtttgccc gaggaggacg aggagccgct cttcctcggt
     1861 cgccgtgccg cccggatcgg gctggttgct caccaccacg gcgccgtcgg tctcgcgagc
     1921 acgctccacg agggacagct gcccggagtg gacgaggccg gtggtcggga cgaagccgat
     1981 ggagtgcccg gctcgtcgga gttcgaggat cgcgtcccgc agctcgtcga cgccgcggac
     2041 gacctccacg cctaggtgtc ctgctggcgc gcgcgacgcg gcgagcggga gcgggtcgac
     2101 cgcgacgcgc gtgcagggcc gctgggcgta tcgccacgag cccgatatcg gtcggcgatg
     2161 agatcccaca gccgctcggc gacctggcgc ttggggagca tcggccagtc ggtcagcgtg
     2221 ccttcccgac cgaagacgac gaccttgttc tcttccgagc cgatcgcgtc gaacgggccg
     2281 ccgaccttgt tggccacgat caggtcgagg cccttgtcgc gcagcttgcc ggtggcgttg
     2341 gcctcgaggt cgtcggactc ggccgcgaag ccgatgagga acgggcgaat ggcttcgggc
     2401 atctcgccga cctcggcaat gatgtccggg ttggggacga gctccagccg gaggctctcc
     2461 gagccgcgct tgatcttctg gccgctcgtc ttggccgggg catagtcggc gaccgcggcc
     2521 gccatgacga ggatgtcggc cgagggagcg atccggagca cggcctcgcg catgtccgat
     2581 gcggtggtcg cgtcgaccac gttcacgccg cggggcgcgg tcaccgacat cgcgccggcg
     2641 acgagggtca ccgccgcgcc acggtcgcgg gccgcctcag ccagggcaac gcccatcttt
     2701 ccgctcgagc ggttgccgat gaaccggacc gggtcgatcg gctcgcgggt accacccgcg
     2761 ctgatcgcca cccgcagccc gtcgaggtcg ccggcgcgcg cgaacaggcg ctccacggcg
     2821 gcgacgaccg cttccggctc ggcgagtcgc ccaatcccgg tcagacccga ggccagcgcg
     2881 ccgacctccg gctcgacgac gaggtagccg aagccgcgga tcgtctccac gttgcgctgg
     2941 gtcgcggggt gggtccacat cccggcgtcc atggccgggg ccacgaccac gcgagcccgg
     3001 ctggcggcga cgatcgcggt caccgcgtca tccaccaggc cggccgcgag ttcgccgagc
     3061 aggttggcgg tggcgggcgc caggacgatg gcgtcggccg cctcggccag ctcgatgtgg
     3121 gcaatgtgct ggtccggatc gagggcgagc acgtcggtga tcaccggccg atgggtgagc
     3181 gacgcgaacg tgagcggggt gacgaaggcg gtcgcggccg gggtcatggc cacgtccacg
     3241 atcgcccccg cgttcatgag ctgacgaacg aggaccgcgg ccttgtaggc cgcaatgctc
     3301 ccgctgaccc cgaccaccac tcggcggccg acgagagtcc cgtcggtgcc ctcggtcacg
     3361 tgcgtgactc cgtgcgggcg gccgccagcg ccgaggcgta gcggacgccg cgcagggtca
     3421 gctccggctc gacccggtcg atgccgggca cgtcggcgag ccatggctgc agggcgaatc
     3481 cgccggtggc gatgaccgtc acccgcgcgt cgcgcggcga ccgctccacc agctcgccgc
     3541 ggatggcggc caggaggccg ctcagcaggc cgatgtagcc gttgaccgtg ccgctctgca
     3601 tggccgcgat cgtattggac ccgatcgcct gcgaggggcg cacgagctcg atccgcggca
     3661 gcttcgaggt tcgtcccacg agcgcgtcaa ccgagatccc gagcccgggc gcgatggcgc
     3721 caccgatgta cgcgccctcc gcgctgagga cgtcaaagtt ggtgctcgtg cccatatcga
     3781 tcacgatggc cggtccgtcg aactcctcgc gtgcggcgag cgcgttggcc agccgatcgg
     3841 cgcccgcttc ggcggcacgg tcgatctcga tggccaggat cccgtccagc gccgcggcgt
     3901 ccacgatgaa cggccgaacg cgagcccggc gggcgagcat ccgggtccat cgggcgacga
     3961 gcggcggcac gacgcaggcc agcgcggccg cgtcgacatc gtccagccgg tggccgtcca
     4021 gggccaggag cccggtgacg gtggccgcga gctcgtcgtc ggtcgcgcgg
//
LOCUS       DASYGU010000256         4307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1070275, whole genome shotgun sequence.
ACCESSION   DASYGU010000256 DASYGU010000000
VERSION     DASYGU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070275"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGO32_07250"
     CDS             <1..193
                     /locus_tag="VGO32_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200584.1"
                     /translation="TVARLLSPEGLLVGVTYTPWMHPHEHGDHEGHDHGEHDHDDHEG
                     HDHEGHDHGEHEGHNHGDH"
     gene            156..644
                     /locus_tag="VGO32_07255"
     CDS             156..644
                     /locus_tag="VGO32_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7200585.1"
                     /translation="MASTRATTTAITEDRLLILGPDEGTAYPGDVLQKVEASQTGGHW
                     GVAVVWGVPGEGGQTHIHDGEAEAFFILEGTVDFLGATSRTPLGPGSFVLVPPDTEHG
                     VRITGSGPARWLAIWPAALDGMFSEIGSIEDVNQEAYDQVRLRHGVRAGRDRRDEFAG
                     QG"
     gene            702..1031
                     /locus_tag="VGO32_07260"
     CDS             702..1031
                     /locus_tag="VGO32_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV7200586.1"
                     /translation="MAKKPSTDELSPFATPALVVLAVLADGPKHGYVIMQESERLSGQ
                     AMGPGTLYAALARLERRGLIEAVPSDDRRRPYRLTGLGATTLQAHLDQLQSFTHQALG
                     RLEGGAA"
     gene            1028..1744
                     /locus_tag="VGO32_07265"
     CDS             1028..1744
                     /locus_tag="VGO32_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200587.1"
                     /translation="MSPRRLVALYPVAWRDRYGDEFLAVLEQRPPRRFDVIDIAWGAL
                     DAHLFPQAPEGRFRMFTRFAGLAAIGAGAALSVGFWGLNEITVPVFYVLAIVGLVGIH
                     LRQVSVRPGLAWFGFLVALLALVWGIGFVLLTKVGVIPTSGGEFGYFASIALWIGSAT
                     LGAVMLAIRVFPTVAGLAFTIAAPVAMIGLLAGRTIAPGSVLEIVSQVGLVIYALAWI
                     VVGWSLLAAQPQEGVLDATG"
     gene            1810..2481
                     /locus_tag="VGO32_07270"
     CDS             1810..2481
                     /locus_tag="VGO32_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEV7200588.1"
                     /translation="MASEVTDPIEELQQAAEDRWVTGWLAGPTWTRYEELPAQPGDEA
                     PDLELPDTSGGKRRLSEFWAYGPALVIFMRHFGCSCLMERWEALKDELDAFTEAGATV
                     VAVCQAEPERAAAVATRRGYRFPLLCDPDRTAYRAFGLLEGTPAQILHDFPWRPNDMD
                     TAQTMFCEPRRGTERAVVDSPWQLPGEFVVAVDGRIQLAHRYQYCEDFPPKTVLLGAI
                     AAAQR"
     gene            2491..3675
                     /locus_tag="VGO32_07275"
     CDS             2491..3675
                     /locus_tag="VGO32_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7200589.1"
                     /translation="MTTTSVRQIDQLRDSIKGFLDFFNGPFSKLNENSDVANFAVGNP
                     LEKPMPEYVETLRAHLEPRRRNWFAYQVSDPKARIAVARSLSEATGMAWDPEDVAMTN
                     GGFAALAVAMRALVEPDDDVIFFSPPWFFYEFMILAAGATPVRVHLPPPAFEPDLDEL
                     AAAITPRTRALIFNSPQNPSGRVYPIETLTALARTLTDASERIGHPIYLISDEPYRKI
                     LFDGITFHSPAEVYPHTVVTYSYGKQLLAPGQRVGYLAVPPTMPDREEFREAAFVAQL
                     ATGYAFPNALLQHALPDLEPLIIDMPAMERRRDRLVGALREMGYEATMPEGTFYLMAR
                     SPIADDIAFTDILARHRVLVLPGTIVEVPDWFRISLTATDEMVERGIPGFAAAYAEAR
                     SR"
     gene            3710..>4307
                     /locus_tag="VGO32_07280"
     CDS             3710..>4307
                     /locus_tag="VGO32_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7200590.1"
                     /translation="MKTLITNGTIVNADATTSGDVLVDGEQIALIGTDLASTVTADRT
                     VDASGKWVIPGAIDVHTHMELPFGGTFAKDTFETGTRAAAFGGTTTIIDFAVQPRGQS
                     LHDGLDAWHAKADGNATFDYGFHMIISDVRDDVLAEMDTLVDEGVTTFKLFTAYPGVF
                     FSDDGAIFRAMQQTTRNGGLIMMHAENGLAIDVVAGQTV"
BASE COUNT          678 a         1527 c         1393 g          709 t
ORIGIN      
        1 gaccgtggcc cggctcctca gtccggaggg gctgctggtc ggagtcacct acaccccgtg
       61 gatgcacccg cacgagcacg gcgaccatga aggccacgac cacggggagc atgaccacga
      121 cgatcacgag ggccatgatc acgagggtca cgaccatggc gagcacgagg gccacaacca
      181 cggcgatcac tgaagaccga ctgctcatcc tgggtcccga cgaagggacc gcctatccgg
      241 gcgacgtcct gcagaaggtc gaggcatccc agaccggagg ccactgggga gtcgcggtgg
      301 tctggggcgt gcccggggag ggtggccaga cccacatcca cgacggtgag gcagaggcgt
      361 tcttcatcct cgagggcacg gttgactttc tcggagccac gagccgcacg ccgcttggac
      421 ctggcagctt cgtcctcgtc ccgcccgata ccgagcatgg ggtgcggatc acgggctcgg
      481 gtcccgcccg atggctcgcc atctggccgg ccgccctgga cggcatgttc tcggagatcg
      541 gctcgatcga ggacgtcaac caggaagcct acgaccaggt tcgtctgcga cacggggtcc
      601 gggcgggtcg tgatcgacgg gacgagttcg ccggccaagg ttgacatgta tcgtgacacg
      661 atatatcgtg atacgacata cacacccaca tgaggtcagc cgtggccaag aagccatcca
      721 ccgacgagct gagcccgttc gccacgcccg ccctcgtcgt cctcgccgtt ctggccgatg
      781 ggccgaagca cggctacgtg atcatgcagg agtcagagcg gctcagcggt caggcgatgg
      841 gacccgggac cctgtacgcc gccctcgccc gcctcgagcg ccgcgggctc atcgaggctg
      901 ttccgtcgga tgaccgtcgc cgcccgtatc ggctgacggg cctcggggca acgacgctcc
      961 aggcccatct cgaccagctg cagtcattta cccaccaggc gctcggacgc ctggaaggcg
     1021 gcgcggcgtg agcccgcgcc ggctggtggc gctgtatccc gttgcctggc gggatcggta
     1081 tggcgatgaa ttcctcgccg tcctcgaaca gcgcccgccc cgccgattcg acgtcatcga
     1141 catcgcctgg ggcgccctcg acgcgcacct gttcccccag gcgccagaag ggagatttcg
     1201 catgttcacc cgtttcgccg gtcttgccgc tatcggcgcc ggcgccgccc tctcggttgg
     1261 cttttgggga ttgaatgaga tcacggtccc ggtcttctac gtcctggcca tcgtcggcct
     1321 tgtcgggatc cacttgcggc aggtctccgt ccgtccgggc ctcgcctggt tcgggttcct
     1381 cgtcgcgctc ctcgccctcg tctggggaat cggattcgtc ctgctcacca aggtcggcgt
     1441 catccccacc agcggcggcg agttcggcta cttcgcgagc atcgcgcttt ggatcgggtc
     1501 cgccaccttg ggcgcggtga tgctggcgat tcgcgtcttc cccaccgtcg cgggtcttgc
     1561 cttcacgatc gccgcgccgg tggccatgat cggcctgctg gctggccgca cgatcgcccc
     1621 ggggagcgtc ctcgagatcg tctcgcaggt ggggctcgtg atctacgcgc tggcctggat
     1681 cgtcgtcgga tggagcctgc tcgctgccca gccgcaagag ggcgtcctcg acgcgaccgg
     1741 ctgaccggcc tagggggccg tccgtcgccg gggaccaatc cggatgacga tcggcgctac
     1801 ccttcgggca tggcgagcga ggtgaccgac ccgatcgagg agctccagca ggccgccgaa
     1861 gatcgatggg ttacaggctg gctcgctggg ccgacctgga cccggtacga ggagctcccc
     1921 gcacagccgg gtgacgaagc cccggatctg gagcttcccg acacgtccgg tggcaagcgg
     1981 cgcctctccg agttctgggc ctatgggcca gcgctcgtga tcttcatgcg tcacttcggg
     2041 tgctcatgcc tgatggagcg ctgggaggcg ctgaaggacg agctcgatgc cttcaccgag
     2101 gccggcgcca cggtcgtggc ggtctgccag gccgagccgg agcgcgccgc ggcggtcgcg
     2161 acccgccgcg gctatcggtt cccgctcctc tgtgaccccg accgaacggc gtaccgcgcc
     2221 tttgggctgc tggaaggcac cccggcccag atcctgcacg acttcccgtg gcggccgaac
     2281 gacatggaca cggcccagac gatgttctgc gagcccaggc gtggcacgga gcgggcggtg
     2341 gtcgactccc cgtggcagct ccccggggag ttcgtggtgg ccgtcgacgg ccgcatccag
     2401 ctcgcccacc gctaccagta ctgcgaggac tttccgccca agaccgtgct cctcggtgcc
     2461 atcgccgccg cgcagcgcta aggtcttcgc atgaccacca catcggtccg ccagatcgac
     2521 cagctgcgcg acagcatcaa gggcttcctg gacttcttca acggcccgtt ctcgaagctg
     2581 aacgagaact ccgacgtcgc caacttcgcc gtcggcaacc cgctcgagaa gccgatgccc
     2641 gagtacgtgg agacgctccg cgcccatctc gagcctcggc gacggaactg gttcgcctac
     2701 caggtcagcg atccgaaagc gcgaatcgcg gtggcgagga gcctgagcga ggccaccggc
     2761 atggcctggg accccgaaga cgtggccatg accaacggcg gattcgcggc gctggcggtg
     2821 gccatgcgag cgctggtcga gccggatgac gacgtcatct tcttctcgcc gccatggttc
     2881 ttctacgagt tcatgatcct cgcggctggt gcgacccccg tccgcgtcca tctcccgccg
     2941 ccggcgttcg agccggacct tgacgagctg gccgcggcca ttaccccccg cacccgagcc
     3001 ctgatcttca actcacccca gaaccccagc ggccgggtct acccgatcga gaccctgacc
     3061 gccctggccc ggaccctgac cgatgcgtcc gagcggatcg gtcatccgat ctacctcatc
     3121 agcgacgagc cgtaccggaa gatcctgttc gatgggatca cgttccactc accggccgag
     3181 gtgtacccgc acaccgtcgt cacctactcc tacggcaagc agctgctcgc cccgggccag
     3241 cgggtcgggt acctcgcggt cccgcccacc atgcccgatc gcgaagagtt ccgagaggcc
     3301 gccttcgtgg cgcagttggc gaccggctac gcattcccga atgccctgct ccagcacgcc
     3361 ctgcccgacc tcgagccgct gatcatcgac atgccggcca tggagcggcg gcgggatcgg
     3421 ctggtcggcg cgctgcgaga gatggggtac gaggccacga tgccggaggg gaccttctac
     3481 ctcatggccc gctcgccaat cgccgacgac atcgccttca ccgacatcct ggcgaggcac
     3541 cgggtgcttg tcctgcccgg cacgatcgtc gaggtccccg actggttccg gatcagcctg
     3601 accgcgaccg acgagatggt cgagcgaggc attcccggct tcgcggctgc ctacgcggag
     3661 gcgcgatccc gctgatctcc ggcggtttcg cccgcgctac catcggctca tgaagacgct
     3721 catcaccaac ggcaccatcg tcaacgccga tgccacgacc agcggtgacg tcctggtcga
     3781 cggcgagcag atcgcgctca tcggtaccga cctggcctcg acggtcaccg ctgaccggac
     3841 cgttgatgcc agcggcaagt gggtgatccc gggcgccatc gacgtccaca cccacatgga
     3901 gctcccgttc ggggggacgt tcgccaagga caccttcgag accgggaccc gggccgcggc
     3961 gttcggtggg acgaccacga tcatcgactt cgcggtccag ccgcgaggcc agtctctcca
     4021 cgacgggctc gatgcctggc atgccaaggc tgacggcaac gcgaccttcg actacggctt
     4081 ccacatgatc atcagcgacg tgcgcgacga cgtcctggcc gagatggaca ccctcgtcga
     4141 cgagggggtg accaccttca agctgttcac cgcttatccc ggcgttttct tctccgacga
     4201 cggtgccatt ttccgggcca tgcagcagac caccaggaac ggcgggctga tcatgatgca
     4261 cgccgagaac gggctggcca tcgacgtcgt cgccggccag acggtgg
//
LOCUS       DASYGU010000257         8787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_350893, whole genome shotgun sequence.
ACCESSION   DASYGU010000257 DASYGU010000000
VERSION     DASYGU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350893"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VGO32_07285"
     CDS             complement(<1..441)
                     /locus_tag="VGO32_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200591.1"
                     /translation="MSHAPTSIRPMAVEDATTVAALAGQLGYPTTPEQIRVRHAVLAA
                     APDRGSVLVAVDAGSPADVIGWAHVELRDTLVAPATAQLMALVVADGARDRGVGRDLL
                     RAAESWARDHGCEALIVATRVTRTEAHRFYRREGYDLDKTSHIFL"
     gene            complement(438..731)
                     /gene="xseB"
                     /locus_tag="VGO32_07290"
     CDS             complement(438..731)
                     /gene="xseB"
                     /locus_tag="VGO32_07290"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV7200592.1"
                     /translation="MTDATPAQPDDLAARPFDELVAELQRIVQTLEAGRVGLEESIAL
                     YREGLRLHAACEARLKAAELTITELGRRGLAGAEATQPKPAADPDGQETAPPA"
     gene            complement(728..1984)
                     /gene="xseA"
                     /locus_tag="VGO32_07295"
     CDS             complement(728..1984)
                     /gene="xseA"
                     /locus_tag="VGO32_07295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV7200593.1"
                     /translation="MTGPSSGTPLPSTGPRVWRVSDLNRRVRGLLDADQALADIWVEG
                     EVSGPSFPPSGHCFFTLKDAHSQIKAVLFREELARASVRPEHGAQLVCHGRVRAYEPQ
                     GVYQLYVESVTPVGAGDLHAQYEALRAKLAAEGLFDESRKRPLPRWPRRIGVVTSPVG
                     AVWDDICTVLRRRYPMAEVVLSPTAVQGGVAAPAIIRALKRLYAVGDLDLVILARGGG
                     SIEDLWGFNDEHVVRAVVASPVPVVVGVGHESDITLADFAADRRAPTPSAAAEIAVPD
                     GTQLPAILNRLGERAAGAMSARLEAGRRAVRGEGRALTGLQPDLATARQRAAELLDRS
                     HRAASADVERRRLAVTGLRDALRALGPAATLERGYAVARRADGMIIRDPADVAAGDDL
                     EVVVARGIIGSRVTTATPGDMEDLLS"
     gene            complement(1998..2558)
                     /gene="efp"
                     /locus_tag="VGO32_07300"
     CDS             complement(1998..2558)
                     /gene="efp"
                     /locus_tag="VGO32_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV7200594.1"
                     /translation="MISTGDIRKGIVIELDGQPVRVLEWSHIKMARGSAQVRLKIQNV
                     RTGSITERTFQAGTRWPRARVEQRKAQFLYSDGDAYHFMDSESYDQFSISRDLLGDDA
                     QYLKDNTDVFVSSYEGEVLGVDLPVTVDLRVQDTDPGFAGDTATGARKAATLETGLVV
                     QVPLFVNEGDLLRIDTRTGEYQTRIQ"
     gene            complement(2555..3694)
                     /locus_tag="VGO32_07305"
     CDS             complement(2555..3694)
                     /locus_tag="VGO32_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV7200595.1"
                     /translation="MTGADPFRAERPARLAALRELIASLDLDGIAVRAPTTSRYLSGF
                     ALRRGDESTSGYSGALLVTADDAVILADNRYVEQVDVEAPDWERVLTADPMPAELARL
                     VAGRGIRRLGMESDRTPHAVWLGIEAASPGLELVPVEDSLSELRIRKSAAEIEAIGRA
                     CELGDAAFAYLCDTIRPGMTERSVARLISGYFEDHDAEDLAFDSIVLVGARASMPHGT
                     PGDARVEAGKPLLLDFGCQLDGYRSDMTRTVFVGEPDDRSRRQHEAVAEAQQAALRLV
                     TVGAPASAPHEAARASLAEAGYPNAFSHGIGHGIGLDTHEPPSLKSSPAPLQAGMVFS
                     IEPGLYLPGEIGVRIEDIVALEATGPRLLTRSPRDAVVIGEGIAA"
     gene            complement(3697..5013)
                     /gene="mltG"
                     /locus_tag="VGO32_07310"
     CDS             complement(3697..5013)
                     /gene="mltG"
                     /locus_tag="VGO32_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV7200596.1"
                     /translation="MYDPSLTPAEQEQLRNARIRRLRSEREANERHRGVRRLQPIVLL
                     GWIGGVIVLTVIALYLGVLALSPAIMSWIEERPTLIQNDLVVDFVNWYEPEAIADTPA
                     STTYERTSVEIPPGATDTFIGQLLVSEGLIHSELAFHYQVYQANREGDLHAGQYDLSP
                     TFLPSFIIGALRQESGPEVTITILEGLRLEELVAYLGTTELTMDLDEFRTLITTPPAD
                     LLAAYPFLTELPAGRTLEGYLYPDTYRLFANATARDVLDRLLSTFDERVTQDIRDQLA
                     ARGLTIDFAVRLGSIVEREAVLDQERPLISGVYTRRLGTAGWRLDADPTLQWGLATAE
                     FGTLPPPEWGSVSWWEPLPVGGAEVVLPEALLPYQTYQVTGLPPTPIASPRISSIQAA
                     AFPDMADGYFFFVAACPNGVRDGSHRFAQTLAQHEANIAQAQAECP"
     gene            complement(5013..5417)
                     /gene="ruvX"
                     /locus_tag="VGO32_07315"
     CDS             complement(5013..5417)
                     /gene="ruvX"
                     /locus_tag="VGO32_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV7200597.1"
                     /translation="MIGVDHGLRRVGVAVGDDETGMAFPRPVLKATNAATDVRRLADQ
                     EGTRRVVVGLPLNADGSEGRQAAIARGFGHALVELGLEVTFSDERLTSWAAEQEAGSA
                     DRPNPSNAIDSAAAALILEQYFADRRRRPEES"
     gene            complement(5452..8103)
                     /gene="alaS"
                     /locus_tag="VGO32_07320"
     CDS             complement(5452..8103)
                     /gene="alaS"
                     /locus_tag="VGO32_07320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV7200598.1"
                     /translation="MSATMPPLSAAEIRERFQRFFEARDHTRVPPAPLLARDDPTLLF
                     TNSGMVQFKRVLTGEESRDYRRAVDSQPCLRVAGKHNDFEEVGRTPRHQTLFEMLGNW
                     SFGDYFKTEAIHWAWEFLTDDLGIAPDRLAATVYSDDDDAHRIWATEIGLPPERLVRW
                     GNIAAGDDHNFWQMADVGPCGPCSEIHYDRGAELSEGPDCVPDHSETCPRWLEIWNLV
                     FMQYDRAADGTLTPLPFQSVDTGMGLERTASVMQGVDSNYRTDLFVPIIDRLAEHLGH
                     RPEEVEAERFSYQVVADHSRAMTFLIAEGVHPTNDGAGYVLRRIMRRAVRHVRLMGIS
                     APVLGETCAAVIDLMGDAYPTLAERREPILDEVRAEEARFARTLEAGSERLTQLVEAA
                     GPNGRIAGEEAFRLHDTFGFPIDLTIEIAGESGVAVDREGFDTAMSAQRERSRGAQHA
                     GYRSDPELAGLSSTFVGYPNETVAEDLEVLAVRPGDDGSTAVVLAETPFYAEGGGQVG
                     DHGRLVGPRGTLEVTDTQRDGDAIVHHGSLEGTLEMGERVRAEVDRERRLAAARNHTA
                     THLLHRALRNVLGEQAKQAGSWVGPDGLRFDFPSDAPTSRDALTRVEGIVNEQVRNNL
                     EVMPTWMPLAEAQGSGADMFFGEKYLPDSVRVVACGDFSRELCGGTHLAATGQIGSFR
                     ITGESSIGAGLRRIEAVTGAAAETLITTRLEALRATAHLLGARDDEVPARVEALQARV
                     RDAERGDRAGTSARPRLDAAAALRDGQDAGDAKVIVQRYADADANALRTLVDDLREST
                     GRFVAVVAGEADGQPTLVVATSRDLAAEGFDAADVIRRVAPLIKGGGGGRADMAQAGG
                     SDLAGLDGALAEATRLTLESLAALEGA"
     gene            complement(8160..8438)
                     /locus_tag="VGO32_07325"
     CDS             complement(8160..8438)
                     /locus_tag="VGO32_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV7200599.1"
                     /translation="MSGDSGFGSFVAGLVIGGLIGAAIGLLLAPESGEDMRGQVGDFV
                     DERVRDFGDAVNEGKAAAEQARTAMLTALQRETALDAPAEAPAEESAN"
     gene            complement(8488..>8787)
                     /locus_tag="VGO32_07330"
     CDS             complement(8488..>8787)
                     /locus_tag="VGO32_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200600.1"
                     /translation="PLALWTLLVSAINLTLFVAIRGRWGRELPFLVIAAVIGTLAGNF
                     LAALLDVDVLVLGEFHLVGAVIGAQVALITTAAVATLLAGTRGSTGRRDGAGSGR"
BASE COUNT         1273 a         3087 c         3140 g         1287 t
ORIGIN      
        1 caggaagatg tgcgaggtct tgtcgaggtc gtagccctcg cgccggtaga accgatgggc
       61 ttcggtccgt gtcacccgag tggcgacgat cagcgcctcg cagccgtggt ctctggccca
      121 cgactccgcc gcgcggagca gatcccggcc gacaccacga tcgcgcgcac catcggccac
      181 gacgagggcc atgagctgcg cggtggccgg cgcgacgagc gtgtcccgga gctcgacgtg
      241 ggcccagccg atcacatcgg ccggactgcc ggcgtcgacc gccaccagaa cgctgccgcg
      301 atcgggagcg gccgccagca cggcgtgtcg cacgcgaatc tgctctggag tcgtcggata
      361 gcccagctgg ccggccagtg ccgcgaccgt ggtcgcgtcc tcgaccgcca tcggtcgaat
      421 ggaggtcggt gcgtggctca cgcgggcggg gcggtctcct gcccgtcggg atcggcggct
      481 ggcttgggct gagtggcctc ggcaccggct agcccacgcc ggccgagctc ggtgatcgtc
      541 agctcggcgg ccttcagccg cgcctcgcag gccgcgtgca gccgcagacc ttcgcggtac
      601 agggcgatgc tctcctccag ccccacgcgt cccgcctcaa gggtctggac gatgcgctgg
      661 agctcggcca ccagctcgtc gaacgggcgt gcggcgaggt cgtcgggctg ggcgggcgtc
      721 gcatcggtca cgagagcagg tcctccatgt ccccgggcgt ggcggtggtc acgcggctgc
      781 cgatgatgcc gcgcgcgacg acgacctcga ggtcgtcccc cgccgcgacg tcagccgggt
      841 cacggatgat catgccatcc gcgcgacgtg ccactgcgta gccgcgctcc agcgtggccg
      901 cgggtccgag ggcccgcagc gcgtcgcgca ggccggtgac ggccaggcgg cggcgctcga
      961 cgtccgcgct cgcggcccgg tgggatcgat cgaggagctc ggccgcgcgc tggcgggccg
     1021 tggccaggtc gggctggagg ccggtcaggg cgcgtccctc cccgcggacg gcccggcgtc
     1081 cggcttccag gcgggcggac atcgcccccg ccgcccgctc gccgagacgg ttcaggatgg
     1141 ccggcagctg cgtgccgtcc gggaccgcga tctcggccgc ggccgatggc gtaggcgctc
     1201 ggcgatcggc tgcgaagtcg gccagggtga tgtcgctctc gtgcccgact ccgaccacga
     1261 ccgggaccgg tgaggcgacg accgcccgca cgacgtgctc gtcgttgaat ccccacaggt
     1321 cctcgatcga gccgccaccg cgggccagga tgacgagatc gaggtcgccg accgcgtaca
     1381 gccgcttcag ggcgcggatg atcgccggcg ccgccacgcc gccctggacg gcagtcggcg
     1441 acaggaccac ttcagccatc ggatatcggc ggcggaggac ggtgcagatg tcatcccaca
     1501 ccgcgcccac tggcgaggtc acgacgccga tccggcgcgg ccagcgcggg agcggacgct
     1561 tgcgagactc gtcgaacagc ccttcggccg ccagtttggc gcgaagcgcc tcgtactggg
     1621 cgtggaggtc gccggcgccg accggggtga ccgactcgac atagagctgg tacacgccct
     1681 gtggctcgta ggcacggact cgcccatggc acacgagctg ggctccgtgc tcgggccgaa
     1741 cggaggcacg cgccagctcc tcgcggaaca ggaccgcctt gatctggctg tgcgcgtcct
     1801 tcagggtgaa gaagcagtgg cccgaaggcg ggaagctcgg accgctgacc tcgccctcga
     1861 cccagatgtc ggccagcgcc tggtccgcgt cgagtagccc acgcacccgg cggttgaggt
     1921 cggaaacgcg ccagacccgt gggccggtcg acggtagtgg tgtgccggac gacggccccg
     1981 tcatcggtct ctcggttcta ctggatccgg gtctggtact cgccggtccg ggtgtcgatc
     2041 cggaggaggt cgccctcgtt cacgaacagc gggacctgga cgacgaggcc ggtctcgagc
     2101 gtggccgcct tccgggcacc ggtggccgtg tcgccggcga agccgggatc ggtgtcctgc
     2161 acgcgaagat cgacggtgac cggcagatcc acgcccagca cctccccctc ataggaggac
     2221 acgaacacgt cggtgttgtc cttgaggtac tgcgcgtcgt cgccgagcag gtcgcggctg
     2281 atcgagaact ggtcgtagct ctcgctgtcc atgaagtggt acgcgtcgcc gtcggagtac
     2341 aggaactggg ccttgcgctg ttcgacccgc gcccgcggcc accgagtacc ggcctggaac
     2401 gtgcgctcgg tgatcgaccc ggtccgaacg ttctggatct tgagccggac ctgggccgat
     2461 ccgcgcgcca tcttgatgtg cgaccactcg agcacccgga cgggctggcc gtcgagctcg
     2521 atcacgattc ccttgcggat gtcgccggtg ctgatcacgc cgcaatcccc tccccgatca
     2581 cgaccgcgtc gcggggcgac cgggtgagga gccggggccc cgtcgcctcc agcgcgacga
     2641 tgtcctcgat ccggacgccg atctcaccgg gcaggtacag gccgggctcg atgctgaaca
     2701 ccatcccggc ctggagcggg gcgggcgagg acttcagcga cggcggctcg tgggtgtcca
     2761 aaccgatgcc atggccgatc ccatggctga acgcattcgg gtagcccgct tcggccagag
     2821 aggcccgggc cgcctcgtgg ggcgcggacg cgggcgctcc gaccgtgacg agccggagcg
     2881 cggcctgctg cgcctcggcg accgcctcgt gctggcggcg ggagcggtcg tccggctcgc
     2941 cgacgaacac ggtgcgggtc atgtccgacc ggtagccgtc cagctggcag ccgaagtcca
     3001 gcaggagtgg cttgccggcc tcgactcggg cgtcgccggg cgtgccgtgc ggcatcgagg
     3061 cccgggcgcc gaccaggacg atgctgtcga aggccaggtc ctcggcgtcg tggtcctcga
     3121 aatagccaga gatgagccgg gccaccgatc gctcggtcat gcccggccga atcgtgtcgc
     3181 acaggtaggc gaatgcggca tcgcccagct cacatgcgcg accgatggcc tcgatctcgg
     3241 ccgcgctctt ccggatgcgc agctccgaca gcgaatcctc caccggaacg agctcgagcc
     3301 ccgggctggc cgcctcgatc ccaagccaga cggcatgcgg ggttcgatcc gactccattc
     3361 cgaggcgccg gatgccgcgg cctgcgacca ggcgtgccag ttcggccggc atcgggtctg
     3421 cggtcagcac tcgttcccag tcgggtgcct cgacgtcgac ctgctccacg tagcggttgt
     3481 cggccagaat cacggcatcg tccgcggtga ccaggagcgc gccggagtag ccggaggtgg
     3541 actcgtcgcc acgacggagg gcaaagcccg acaggtagcg gctggtggtg ggggcgcgaa
     3601 cggctatgcc gtcgaggtcg agcgacgcga tgagctcacg cagagcggcc agccgggccg
     3661 gccgctctgc gcggaacgga tccgcgccgg tcacggtcag ggacactccg cctgcgcctg
     3721 ggcgatgttc gcctcgtgct gcgcaagcgt ctgcgcaaac cgatgcgacc catcgcggac
     3781 gccattgggg caggccgcca cgaagaagaa atagccgtcc gccatgtccg ggaaggccgc
     3841 ggcctggatg gacgagatcc gcggcgaggc gatcggggtg ggcggcagac cggtcacctg
     3901 gtacgtctgg tatggcagca gagcctctgg caggaccacc tcggctccac ctacgggcaa
     3961 cggctcccac cagctgaccg atccccactc cggtggcggc agggtcccga actcggccgt
     4021 ggccaggccc cactggaggg tcgggtcggc gtccagcctc cagcctgcgg tccccagccg
     4081 gcgcgtgtag acgccggaga tgagcgggcg ctcctggtcg agcacggctt cgcgctcgac
     4141 gatcgatccc aggcggaccg cgaagtcgat ggtcaggccg cgcgcggcca gctgatcgcg
     4201 gatgtcctgc gttacccgct cgtcgaaggt ggacagcagt cggtcaagga cgtcacgcgc
     4261 ggtggcgttc gcaaacaacc ggtatgtgtc tgggtacagg tagccctcga gcgtgcggcc
     4321 ggcgggcagc tcggtcagga acgggtaggc cgcgagcagg tcagcgggcg gggtggtgat
     4381 cagcgtccgg aactcgtcga ggtccatcgt cagctcggtc gtcccgaggt atgccaccag
     4441 ctcctcgaga cggaggccct ccaggatcgt gatcgtgact tcgggaccgg actcctggcg
     4501 cagcgcgccg atgatgaacg aggggaggaa ggtcggcgaa aggtcgtact ggccggcatg
     4561 gaggtcgccc tcgcggttgg cctggtagac ctggtagtgg aaggcgagct cgctatggat
     4621 cagaccctcg ctgaccagca gctggccgat gaacgtatcg gtcgcgccgg gcgggatctc
     4681 gacgctcgtc cgctcgtagg tcgtgctggc cggcgtatcg gcgatggcct ccggctcgta
     4741 ccagttcacg aagtcaacga ccagatcgtt ctggatcagg gtcgggcgct cctcgatcca
     4801 ggacatgatc gccggcgaca gggccaggac cccgaggtac agggcgatca cggtgaggac
     4861 gatgaccccg ccaatccagc ccagcagcac gattggttgc agccgccgga cgccgcgatg
     4921 ccgctcgttc gcctcgcgct cggagcgcaa gcggcggatc cgcgcgttgc ggagctgctc
     4981 ctgctcggcg ggcgtgagcg atgggtcgta catcaggact cctcggggcg ccgccgacga
     5041 tcggcgaagt actgctcaag gatgagggcg gcggcggcag agtctatcgc gttcgacggg
     5101 tttgggcgat ccgcggaccc ggcctcctgc tcggcagccc aggacgtcaa tcgctcgtcg
     5161 ctgaaggtga cctccaggcc gagctcgacc agcgcgtggc cgaatccgcg ggcaatggcg
     5221 gcctgccggc cttcgcttcc gtccgcgttc agcgggaggc ccaccaccac gcgtcgcgtg
     5281 ccctcctggt cagccaggcg gcggacatcg gtcgcggcat tcgttgcctt cagaacaggt
     5341 cgcggaaagg ccatccccgt ctcatcgtcg ccgaccgcga cgcccacccg cctcagcccg
     5401 tggtcgaccc cgatcacgcg ccccacgtcg cccccggccg tccgagcacg cctatgcccc
     5461 ctcgagcgcc gcgagggact cgagcgtgag gcgggtcgcc tcggccaggg cgccgtcgag
     5521 ccccgcaagg tccgagccac cggcctgggc catgtccgcg cggccgcccc cgcccccttt
     5581 gatgagcggc gcgacccggc gaatgacatc cgcggcatcg aacccttcgg cggcgaggtc
     5641 gcggctggtg gcgacgacca acgtgggctg tccatcggcc tcgccggcga caaccgccac
     5701 gaaccgtccg gtggattcgc gcaggtcgtc gaccagggtc cgcagcgcgt tcgcatcggc
     5761 atcggcgtag cgctggacga tgaccttcgc gtcgccggcg tcctgcccgt cgcgcagcgc
     5821 ggccgcggcg tcgagccggg gtcgcgcgga ggtccccgcg cggtcaccgc gttcggcatc
     5881 gcggacacgg gcctggagcg cttcgacccg ggccgggacc tcgtcgtccc gggcaccgag
     5941 gagatgcgcc gtggctcgca gcgcctcgag ccgggtggtg atcagcgttt ccgcggccgc
     6001 cccggtcacc gcctcgatcc gccgcagacc ggcaccgatg ctggactcgc cggtgatccg
     6061 gaacgatccg atctgtccgg tcgccgcgag gtgggtgccg ccgcacagct cgcgcgagaa
     6121 gtcgccgcac gcgacgaccc gcaccgagtc gggcaggtac ttctcgccga agaacatgtc
     6181 cgctcccgag ccctgcgcct cggccagcgg catccaggtc ggcatgacct ccaggttgtt
     6241 gcggacctgc tcgttcacga tgccctcgac tcgggtgagc gcatcgcgcg acgtcggcgc
     6301 gtcggacggg aagtcgaacc gcagcccatc gggcccaacc cagctccctg cctgcttggc
     6361 ctgctcgccc aggacgttgc gaagcgcccg atggaggaga tgggtggccg tgtggttgcg
     6421 ggccgccgcc agccgccgct cgcggtcgac ctcggcgcgg acccgctccc ccatctcgag
     6481 cgtcccttcc agcgacccgt ggtggacgat cgcgtcgcca tcccgctggg tgtcggtcac
     6541 ctcgagcgtc ccgcgcggcc cgaccaggcg cccgtggtcg ccgacctggc caccaccctc
     6601 ggcatagaac ggggtctcgg ccaggacgac ggccgtggag ccgtcgtcgc cggggcggac
     6661 ggcgaggacc tcgagatcct ccgccaccgt ctcgttcgga tagccgacga aggtcgagct
     6721 caggccggca agctcggggt cggatcggta tccggcgtgc tgcgcgcccc gcgaccgctc
     6781 gcgctgggcg ctcatcgccg tgtcgaagcc ttcgcggtcg accgcgacgc cggattcccc
     6841 cgcgatctcg atcgtgaggt cgatcgggaa gccgaaggtg tcgtgcagtc ggaatgcctc
     6901 ctcgcccgcg atccggccat tcgggcctgc cgcttccacg agctgggtca gccgctcgga
     6961 gcccgcttcc agggttctcg cgaagcgggc ctcctcggcc cggacctcgt ccaggatcgg
     7021 ctcgcgccgc tcggcgaggg tcgggtaggc atcgcccatg aggtcgatga ccgccgcgca
     7081 cgtctcgccc agcaccggcg cgctgatccc catcaggcga acgtggcgca ccgcgcggcg
     7141 catgatccgg cgcaggacgt acccggcacc gtcgttcgtg gggtggacgc cctcggcgat
     7201 caggaaggtc atcgcccgcg agtggtcggc gacgacctgg tagctgaacc gctcggcctc
     7261 gacctcttcg gggcggtggc cgaggtgctc ggccagacga tcgatgatcg gcacgaacag
     7321 atcggtccgg tagttgctgt cgacgccctg catgaccgac gccgtgcgct cgagccccat
     7381 cccggtgtcc acgctctgga acgggagcgg ggtcagcgtt ccgtctgccg cgcggtcgta
     7441 ctgcatgaag accaggttcc agatctcgag ccagcgcgga cacgtctcgg agtgatccgg
     7501 gacgcagtcc gggccctcgc tcagctcggc gccgcggtcg tagtgaatct cactgcacgg
     7561 accacagggg ccgacatcgg ccatctgcca gaagttgtga tcatccccgg ccgcgatgtt
     7621 cccccagcgg acgagccgct cggggggcag accgatctcg gtcgcccaga tccggtgcgc
     7681 gtcgtcgtca tcggagtaga ccgtcgccgc cagccggtcg ggcgcgatgc caaggtcatc
     7741 ggtcaggaac tcccaggccc aatggatggc ctcggtcttg aagtagtcgc cgaagctcca
     7801 gttgccgagc atctcgaaca gggtctggtg gcgcggcgtc cggcccacct cctcgaagtc
     7861 gttgtgcttg cccgccacgc ggaggcaggg ctggctgtcg accgcccgcc gatagtcgcg
     7921 ggactcctcg ccggtgagga cccgcttgaa ctggaccatc cccgagttcg tgaacaggag
     7981 cgtcgggtcg tcgcgggcca ggagtggggc cggcgggacg cgggtgtggt cgcgcgcctc
     8041 gaagaagcgc tggaagcgct cgcggatctc cgcggcggac agtggcggca tggtggcgct
     8101 catgcgcgtc gagagtcgac ccccgccgac cctcctccgt atgggattgc caacggaggt
     8161 tagttcgcgc tctcctcggc cggggcttca gccggagcgt ccagcgcggt ctcacgctgg
     8221 agggcggtga gcatggccgt ccgggcctgc tcggccgcgg ccttgccttc gttcaccgcg
     8281 tcgccgaagt cacgcacgcg ctcgtcgacg aagtcgccga cctggccgcg catgtcctcg
     8341 cctgactcgg gggccagcag cagtccgatg gcagcgccga tcagaccgcc gatgaccaag
     8401 ccggccacga aactcccgaa accggaatct ccactcatga cgtgtgtgtc gctcctgtag
     8461 gggacatcgc tggtagggcc accgattcta ccggcccgaa cccgccccgt cgcgtcggcc
     8521 cgtggatccg cgggttcccg cgagcaatgt ggcgaccgct gcggtggtga tcagcgcgac
     8581 ctgcgcaccg atgacggccc cgaccaggtg gaactcgccg aggaccagga cgtccacatc
     8641 gagcagcgcg gcgaggaagt tgccggcgag ggtgccgatc accgcggcga tgaccaggaa
     8701 tggcagctcc cgaccccagc ggccgcggat cgcgacgaac agggtcaggt tgatggcgga
     8761 aaccagcagg gtccagaggg ccagcgg
//
LOCUS       DASYGU010000258        16447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1584643, whole genome shotgun sequence.
ACCESSION   DASYGU010000258 DASYGU010000000
VERSION     DASYGU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16447
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584643"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGO32_07335"
     CDS             <1..537
                     /locus_tag="VGO32_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7200601.1"
                     /translation="PDVVLMDLTLPAPNGIETTQRIKRELPHTGVIVLAGGDDEDQLF
                     DAIKAGAAAYVLKDIDPTDLVAIIRRVRSGEYLINDKVFSRPAVASRVLKEFRELAVY
                     GSDAQPIFAPLSPREVEILDNIAQGMTNKQVAYTLGISEQTVKNHMSSILRKLSVNDR
                     TQAVVYAMRQGWIKLPED"
     gene            960..1139
                     /locus_tag="VGO32_07340"
     CDS             960..1139
                     /locus_tag="VGO32_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEV7200602.1"
                     /translation="MLQFISTLLARFSHEDEEGQTLVEYGLLLALIAIIVIVALLFLG
                     PIVSQIFSNVAQNLQ"
     gene            1380..2252
                     /gene="cpaB"
                     /locus_tag="VGO32_07345"
     CDS             1380..2252
                     /gene="cpaB"
                     /locus_tag="VGO32_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEV7200603.1"
                     /translation="MKRSNRLVILVGVLLAVLAFVGVLFVLNQSGGPTGEGPDVTATV
                     LVATEDIEIGDEVTPEMVEAAQVDPAAVVGTPFSDPSQLTGRAAIQAVASGGQVSQET
                     VGAVANPEVCISCSLLPGEKAIAFQVDRVTGLDFLVLPGDHIDVVLNQRIPVVQPTIE
                     TVNSPPDQQRFEGVAGLEAAPTVKTVLQNKRVIYVSQARNLASAEASPGTDGGGEAAV
                     EISSVIIIFAGTDQDAELIKLAQSDVTLTGALTAIVRAADDDVNEATTGVTISLLIER
                     YGVPVPNIVLLQSE"
     gene            2279..3499
                     /locus_tag="VGO32_07350"
     CDS             2279..3499
                     /locus_tag="VGO32_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7200604.1"
                     /translation="MIKVLIVDDIAETRDHLTKLLAFEQTVEVAGAAGSGEEAITMAM
                     DLRPDVVVMDINMPGMDGIAAAELISQRLPTIAIIMMSVHGEAEQMRRSLQAGAREFL
                     VKPFSSDEFSETIKRVYDREEARRHQIQAAMPAGPATAEADPDVEHQVIGIFSPKGGS
                     GRTTIATNVAIALKQESGARVALLDANLQFGDVGVLLNLNPKTRSIVDLADGGEPDAD
                     LIESVMIEHSTGVRALLAPPTPEGADLITPAYLKTVVEQLRTMHAYTVVDLPSGLNDH
                     SLAIMDMADQIVIMAALEITAIKNLRLFLEVADQLGYERSKLRIVMNRSDATQGIRMG
                     DVESSIRRPIDGTIISDGRLAVLAVNRGVPFIVSNPESPLSRDVYKLAQTLIGGVTTS
                     TDEKPNRRGIFARR"
     gene            3516..4934
                     /locus_tag="VGO32_07355"
     CDS             3516..4934
                     /locus_tag="VGO32_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEV7200605.1"
                     /translation="MSLLKRIERAQPGQESDLAPVPAGTPTPATTPGGPAAPTGEGPA
                     RPFAGGAPTRESFREAKYRVQNRLINELDPKMDLSNQVEVKRQIEDLFGKIADEEGLA
                     LTRAERVRMLEQITDEILGLGPLEPLLRDATITEVMVNGPQQVYIEREGKLELTGVTF
                     QNDEHVMKIIQRIIAPIGRRIDESSPMVDARLVDGSRVNAIIPPLSLVGPVVTIRKFA
                     ASPFTVEDLIRFGTSTAEMFEFLEACVKARLNVFVSGGTGSGKTTTLNVLSSFIPDDE
                     RIVTIEDAAELQLRQEHVVTLEARPSNIEGKGAVPIRELVRNALRMRPDRIVVGEVRS
                     AEALDMLQAMNTGHDGSMSTGHANSPRDMLARLETMVLMAGMDLPLRAIREQTASAVD
                     LIVHQNRLKDGTRKIVSITEVQGMEGDVIVMQDVFTFEQTGVVEGKIEGRLKPTGIRP
                     KFVEKFETAGIHLPANIFGSPF"
     gene            4959..5957
                     /locus_tag="VGO32_07360"
     CDS             4959..5957
                     /locus_tag="VGO32_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV7200606.1"
                     /translation="MNLLPIIIAGLGAMAIVFVTLGIAMSGGGGTVTDRLERYASSRG
                     GRSDKDDDPERESAVIAGLSKVIERQDMTARLSTDLARADLKLKPAEFILIWIATPFA
                     LLFLGAVLGLTLIPALQTPLALVALFAVGFWLPRFYLGRRQRGRLQAFNDQLPDTITL
                     LANSLRAGSSFLQGMELVTREARPPIAEEFGRVVREMSLGLALQPALANLVRRVASED
                     LELMVTAINIQAQVGGNLATVLDAIAFTIRERVRIQGEIRTLTAMQRYSGYVIVLLPV
                     GLAALLFVISPTYMTPMLERPPEMLGLPMGVVLLLLGLLSMGIGWLFIRRIVDIKV"
     gene            5962..6885
                     /locus_tag="VGO32_07365"
     CDS             5962..6885
                     /locus_tag="VGO32_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV7200607.1"
                     /translation="MFENPLLVLIAGLAAGAVLFIIFGAVSRPPKDAVQQRLEQLVVK
                     PKTLEEAELELPFFERAVRPVVQRVSKMGRRGGGVNMIERAEQRLHAAGYPGGLRGAD
                     WMGVKVLAAIVFAIAFTIIFLGVSGFPTGILFGLGGLAVGFLAPEFWLGRKIRGRQME
                     MILQLPDALDLLTISVEAGLGFDAALAKVVEKMDGPLVAEFRQALAEIRMGRLRREAL
                     RDVATRADVQPVTNFIGAIVQAEQLGVPIAKVLQIQSQQLRIERRQRAEEAAAKAPVK
                     MLFPMVGCIFPTIFIVILGPAIVTVVRGVGI"
     gene            6887..7579
                     /locus_tag="VGO32_07370"
     CDS             6887..7579
                     /locus_tag="VGO32_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7200608.1"
                     /translation="MQPALRPAILLVDDDATLLSVLSRHLTREGFAVRTASGGQAALA
                     SLSQSWPAMVVVDLMMPGMDGFELSQRIKRTADLPIIVLSAVDADETKIDALQHFAED
                     YVVKPFSPDELVARIHRVLRRAAPSRTQVSIGDGDLEIDLAGRRVATPAGSHPLTPTE
                     VRFVQVLLGSLDRTVPTETLLDRVWSEADGADASYVWVTVRRLRRKLELDPDRPRFLL
                     TERGIGYRLASG"
     gene            7766..8353
                     /locus_tag="VGO32_07375"
     CDS             7766..8353
                     /locus_tag="VGO32_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEV7200609.1"
                     /translation="MRRHDSERGQTLVEFALVFPIFILLIVGLIEFSLAFNALLSVNF
                     ASRDAALLAAEAGEDSGADCVVLASIDEDVQPPASRVRISEVRIYWADSQGNELDAQV
                     YRRNSGAPTNCNVPGLPATLPYTLQGGSSYPENERCSVLAGCPEDPGHTELDNIGVSI
                     TYSYSWVTPMAKIISLGGTGFTLTHANVMRMEPVL"
     gene            8350..8973
                     /locus_tag="VGO32_07380"
     CDS             8350..8973
                     /locus_tag="VGO32_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEV7200610.1"
                     /translation="MSALRRLHARFRSGAERGQAMLEMALILPVLLLIVLGTLEFGFV
                     MDHKLTLEYASREAARVGSALADGGGSPGCLTVDNEIIAAAQRILESSDSNIEIAAVS
                     QIRIYRADLGPGPDGREDVGSDPVNVWLYQPGAGPTVDGVPLDFAPTGAPGWNACGRI
                     NTGVNPESIGVSVTYTYVSRTPLQAIMNLLQIGMYDFTVFELNPTNN"
     gene            8983..11565
                     /locus_tag="VGO32_07385"
     CDS             8983..11565
                     /locus_tag="VGO32_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEV7200611.1"
                     /translation="MPTIRTQSITDEAQPRMTSRGNRPRGQVLVLFALLLTFLLGTAA
                     FVVDLAWIWVNELKVQRAADAAALAGVVFLPGNPVSAGTSAHEESRKNGYLDGDGTIT
                     AAPNGGVDVWARPDSLDPRRMLVTVSAPIETFFMKIFGFDQLQVTRDAKAEFILPVPM
                     GSPENYYGVFGALRTPDGGVWQPNASTTLGPLTPRNVLSSSTAAPGTNQNNNADSCNT
                     NAAASWGIFPTTGAAPQQDDRVNSSNNQWMRSPRINDRVCLNDFGFTFPANPATPPGA
                     PAYPGHVVDGIEVRLETFASDTSGCELRVDLMWGAAGSGGTNNTNTTGTGDKILAVQG
                     TEATGAPYTVLGGASDTWTRAWPLSDITANTFRVRVWNTDPAACSTLTAGQPAAGIRT
                     PNSPPATAPSAPVAGEVYVDLVQVVIHYHTYTWIPDPQVRSPYNQALNARGLWGTFIN
                     QGAEKVNGDAYLPKWDPRTSGTNAEYNPNQYYNYAIEMPAGATAGEVWIFDPAMCATD
                     TGGQFGTGDRFFGGSTAATSAYYTLWNTFNTPFDLGDDTLANPVSRAHNIFANQQADD
                     DTLFGSGSGPVGTSCTQGATAALNDGRFWHNRWYPMQPYVTGAAAEETPASAEQPWPL
                     LGGGPTSAPTLNMTGGNTYRLHTRTTNPTNPTAMDNANGHNSFSIWTQAWCGGSVCAA
                     APRVYGLGAMEAFTPLPGGGAAQFYLAEIEPIHAGKSLEIRLWDPGDTGSLSADLRIK
                     YPSGGSYHNADMDFIANAVASGAAACNGRSDDTVGPPGPLDDVITTNTGGSSQYNGCW
                     LTIVVVIPDTYDGDPCPGVPPCLDQDWWKIEYTMGGAATDNAFDLTTWQVTLRGNPVH
                     LVVP"
     gene            11695..13221
                     /locus_tag="VGO32_07390"
     CDS             11695..13221
                     /locus_tag="VGO32_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7200612.1"
                     /translation="MDLGQPALRLFAVIAVVALPALAALALVLAFAEGWVAEVGIGTA
                     FLIVGLGTMAWAGIVAAWTARAVSRDLQDVVAIATRGDASDAVGGGDEELRTAQRQLR
                     GALDERNRQIATLAADVAATPITGGPVEVAAGVVTVARQVAGDPTWLLVVLHSSDPAA
                     LPAGVYDDDPATPTRSVDELEQWAAVTGDPARLQPRHLIGPWGAVVVVDISSGDELTG
                     FLLAPWEGRPEPSPAERDLLSLIAQVAASTIEHSLLYARLRAQTDDLNRLAAVQSDFL
                     RGITHDLQTPLTSIRALAADIGQEAELNAQARADLEAIAHQADRLRRMVGQLLAVSRL
                     DAGALEPRQEVFRVEPIVERTWEALRAAERPFTLETDGSTQLAVGDPDRLEQVLWAVL
                     DNAVKYSPPRSAITVRVSATSGPAGMQDGSGGHGLEIAVVDRGRGMTAAIRDQAFDQF
                     FRDDEARRAAPDGSGIGLYAARGLIEAMGGSISLDGGDEEGTVVRIRLPAEPISADES
                     "
     gene            13225..13611
                     /locus_tag="VGO32_07395"
     CDS             13225..13611
                     /locus_tag="VGO32_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEV7200613.1"
                     /translation="MATTWVRVTNPTRGTTLAEHCRMATSMRDRTVGLLGTSALGPGD
                     GLWIERSPSIHMFFMRYAIDAVFVDGDDRVVRIVERLRPWRIVAWARGAKDCIELPAG
                     SVQGTATAVGDVLRRERGEAPVSEAR"
     gene            13657..14319
                     /locus_tag="VGO32_07400"
     CDS             13657..14319
                     /locus_tag="VGO32_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV7200614.1"
                     /translation="MGLLGVLLPPSCAGCGRYGEPICADCRSCLRPLSNGSDQFLAPD
                     PGIVVGEALLLAAAAFAHRDEAQRILRRLKYGGGRRLAAPLAHEAWPAFRRLLAITGP
                     VPLVPVPLHRSRQRDRGYNQAELIARELGRRARAPVWPAICRQRRTERQHGLDRATRM
                     RNLRDAMAFEPPPSSGARPPTTVILVDDILTTGATFEACAVILREAGVAEVYGFAIAR
                     EV"
     gene            14384..14944
                     /gene="raiA"
                     /locus_tag="VGO32_07405"
     CDS             14384..14944
                     /gene="raiA"
                     /locus_tag="VGO32_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV7200615.1"
                     /translation="MQTILHARHLELDARLRARIERKLQRLDRLADPSALATVELTAR
                     ASRASDTALVAAVTLVSGGTTLHSSAAGATPIAAVDAVLDKLERQVVRAKQRLRESRR
                     APVAKAAAARAATTSMSSDGPAETDVVEITRLDMVPMFPEDAISRMEELDQAFFVFLN
                     AQTRRVSVAYRRSNGGHGLIDPIVSR"
     gene            complement(15129..15563)
                     /locus_tag="VGO32_07410"
     CDS             complement(15129..15563)
                     /locus_tag="VGO32_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7200616.1"
                     /translation="MTLQTPRSFNSWLRSQLKEKKMSQRQLALQSGVDHSTISRLIKG
                     DRMPSLGTATKLARGLREIHDESEGPTYFAGLATRQLLPTSRVEYALRGDELLTEADV
                     RELMQAYISVRSRRLRPGMANGGTQPASNGDTPPNSKMRSGA"
     gene            complement(15697..>16447)
                     /locus_tag="VGO32_07415"
     CDS             complement(15697..>16447)
                     /locus_tag="VGO32_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV7200617.1"
                     /translation="VNCLVPAKLGDLYRAFLLKGNYGGSASRTVGTIFIERIADIVVI
                     FGLAVAAGYWSFRGRAMPQVDFIFVAAALLATILVVLLLVLRFAGQHIERFLPNRVEG
                     FYARFREGTIAALTPRAVVVIGSLTVVVWLAEGLRVFFVIKALDLPDAQLGISAAVFV
                     ALVAALLTAIPLTPAGIGFVEAGIVGALTLYGVSPESAVAIALTDRAISILTVIVLGG
                     LDYVRSPLVRRAHSAGLGNPPPPAKQPITET"
BASE COUNT         2702 a         5760 c         5440 g         2545 t
ORIGIN      
        1 ccggacgtgg tcctgatgga cctcaccctg ccggcgccga acggcatcga gaccacccag
       61 cgcatcaagc gagagctgcc gcacaccggc gtcatcgtgc tggccggcgg cgacgacgag
      121 gaccagctgt tcgacgccat caaggcaggg gcagcggcct acgtcctgaa ggacatcgat
      181 cccaccgatc tggtcgccat catccgccgg gttcgttccg gcgagtacct catcaacgac
      241 aaggtcttca gccggccggc cgtggccagc cgtgtcctga aggagttccg cgagctggcc
      301 gtctacgggt cggacgcgca gcccatcttc gcgccgctct cgccgcgaga ggtcgagatc
      361 ctggacaaca tcgcccaggg gatgaccaac aagcaggtgg cctacacgct cggcatcagc
      421 gagcagacgg tgaagaacca catgtcgtcc attctccgca agctgagcgt gaacgaccgc
      481 acccaggccg tggtgtatgc catgcgccag ggctggatca agctccccga ggactgaccg
      541 atgatggcgc gcggcccggc cgtgcgccac tggtccggtg gccgtcggta cgggagtgcc
      601 attcccgtcc acccggcgtg atcgcacgat gcgggcacgt tctcccaggt atctgggagg
      661 gccttcatcg ggcgaggtcc cggccacaga ggccgggcga cctcggggtc tcctcctccg
      721 gtgcaagaga gccgcgcagc agcaggcgcg gctctcttgt tgccccgctg gcgcctccag
      781 ccgagctctc cggcaggctc ccgaggcccc gcccggtgcg gcttgacaac actggacccc
      841 tcgcgcaagg tacggcggcc gcgggggcta ggtaggacca acgggtcata ttcatcgact
      901 cggtcgcggc cctacgctgc cttcgcccaa tcctcgagga aaggaggtac ctgcaaggca
      961 tgcttcagtt catcagcact cttctcgctc ggttctctca tgaggacgag gaaggacaga
     1021 ccctcgttga gtacggtctg ctcctggccc tgatcgcgat tatcgtgatc gtggctcttc
     1081 tgttccttgg ccccatcgtg agccagatct tcagcaacgt cgctcagaac cttcagtagg
     1141 tcaagcgtca ttgcgaccga cgcccccggg cccaggcccg ggggcgtccc tctttgacgg
     1201 ggtatctgtc accgggtccc agggggcgcc agccccgagt agtactcccg tcgcgtagcc
     1261 cacaagtcca gtcgaatgcg ccctgtgtgc ctcgtagact gttgaccgca gtcacaacgc
     1321 tgcgtggact tatccacatc cgttccacat ccctgcacag tgaataggag acgctccccg
     1381 tgaagcgatc caaccgtctg gtgatcctgg tcggcgtcct gctggcggtt ctcgcctttg
     1441 tgggcgtcct gttcgtactc aatcagagcg gcggcccgac cggcgagggg ccggacgtca
     1501 ccgcgaccgt cctcgtcgcc accgaggaca tcgagatcgg ggacgaagtc acccccgaaa
     1561 tggtggaggc cgcgcaggtc gatccggcgg cggtcgtcgg gacgcccttc agtgacccgt
     1621 cgcagctgac cggtcgcgcc gcgattcagg cagtcgcgtc gggaggccag gtgagccagg
     1681 agacggtcgg tgccgtcgcc aatcctgagg tgtgcatctc ctgcagcctg ctcccaggcg
     1741 agaaggcgat cgccttccag gtcgacaggg tcacgggcct agatttcctg gtgctgccgg
     1801 gcgaccacat tgacgtcgtc ctcaaccagc gaatcccggt cgtccagccg acgatcgaga
     1861 cggtgaacag cccgcccgac cagcagcggt tcgagggcgt cgccggcctg gaggccgcac
     1921 cgaccgtgaa gacggtgctc cagaacaagc gcgtgatcta cgtcagccag gcgcgcaacc
     1981 tggccagcgc cgaggcttca ccaggcaccg atggcggcgg ggaagctgcg gtcgagatca
     2041 gcagcgtgat catcatcttc gcgggcaccg accaggacgc ggagctgatc aagctcgccc
     2101 agagcgacgt caccctgacc ggcgcgctga ccgcgatcgt ccgggccgcg gatgacgacg
     2161 tgaacgaagc cacgaccggc gtcacgatct cgctcctcat cgagcgatac ggcgtcccgg
     2221 ttcccaacat cgttctgctt cagtccgagt agacgacggc agagccgagg gcacacgcgt
     2281 gatcaaagtc ctgatcgtcg acgacatcgc ggaaacccgc gatcacctga ccaagctgct
     2341 cgcattcgag cagacggtcg aggtggcggg ggcggccgga tcgggcgagg aagccatcac
     2401 catggcgatg gatctccgcc ccgatgtcgt cgtcatggac atcaacatgc ccggcatgga
     2461 tggcatcgcc gctgcggagc tgatctccca gcggctgccg accatcgcca tcatcatgat
     2521 gagcgtccac ggcgaggcgg agcagatgcg acgctcgctg caggccggcg cgcgggagtt
     2581 cctcgtcaag ccgttctcga gcgacgagtt cagcgagacg atcaagcgcg tctacgatcg
     2641 ggaagaggcg cgccgccacc agatccaggc ggccatgccg gcgggaccgg ccacggccga
     2701 ggccgatccg gacgtcgagc accaggtcat cggcatcttc tcgcccaagg gcgggtccgg
     2761 gcggaccacg atcgcgacca acgtcgccat cgccctcaag caggagtcag gtgcccgggt
     2821 cgctctcctc gacgcgaacc ttcagttcgg ggacgtgggc gtcctcctga acctgaaccc
     2881 caagacgcgt tcgatcgtcg atctcgccga tggcggtgag ccggacgccg acctcatcga
     2941 gtcggtgatg atcgagcact cgaccggggt gagggcgctc ctcgccccgc ccacgccgga
     3001 gggcgcggac ctcatcacgc cggcctacct gaagaccgtg gtcgagcagc tccgaacgat
     3061 gcacgcatac accgtcgtgg atctcccatc cggcctgaac gaccactcgc tggcgatcat
     3121 ggacatggcc gaccagatcg tgatcatggc cgccctcgag atcaccgcga tcaagaacct
     3181 gcggctcttc ctcgaggttg ccgatcagct cggatacgag cggtcaaagc tccgaatcgt
     3241 catgaaccga tcggacgcga cccagggtat ccggatgggc gacgtggaaa gctccatccg
     3301 tcggccaatc gacggcacga tcatttccga tggtcggctc gcggtcctcg ccgtgaaccg
     3361 cggggtgccg ttcatcgtct cgaaccccga aagcccgctc tcgcgggacg tgtacaaact
     3421 tgcccagacc ctgatcggcg gcgtcaccac gtcgaccgac gaaaagccaa accggcgtgg
     3481 aattttcgcg cgccgctgac gagaaggatg ctgacatgtc tctcctgaaa cgtatcgagc
     3541 gagcccagcc agggcaggaa tccgatctgg cacctgtgcc ggccggaacg cccaccccgg
     3601 cgaccacgcc gggaggtccg gctgccccga ccggggaggg tccggcacgg ccatttgccg
     3661 gcggcgcgcc gacacgcgag tcgttccggg aggcgaagta ccgcgtccag aaccgactga
     3721 tcaacgagct cgacccgaag atggacctca gcaaccaggt cgaggtcaag cgccagatcg
     3781 aggacctgtt cggcaagatc gccgacgagg aaggtctggc cctgacccgc gccgagcggg
     3841 tccgcatgct cgagcagatc accgacgaga tcctcggtct gggccctctg gagccgctgc
     3901 tccgggacgc gaccatcacc gaggtcatgg tcaacggacc gcagcaggtt tacatcgagc
     3961 gcgagggcaa gctggagctg accggcgtca cgttccagaa cgatgagcac gtgatgaaga
     4021 tcatccagcg catcattgcc cccatcggcc gtcggatcga cgagtcgtcg ccaatggtcg
     4081 acgcccggct ggtcgatggt tcccgcgtga acgcgatcat cccgccgctg tccctggtcg
     4141 gaccggtggt gaccattcga aagttcgccg cgagcccgtt caccgtcgag gacctcatcc
     4201 gattcggtac ctccaccgcg gagatgttcg agttcctcga ggcctgcgtg aaggcccggc
     4261 tcaacgtctt cgtgtcaggc ggtaccggct cgggcaagac caccaccctg aacgtgctga
     4321 gctcgttcat cccggatgac gagcggatcg tgaccatcga ggacgcggca gagctccagc
     4381 tccggcagga gcacgtcgtg acccttgagg cgcgcccctc gaacatcgag ggcaagggcg
     4441 cagtccccat ccgcgagctc gtccgcaacg ccctccgtat gcgtcccgac cggatcgtgg
     4501 tcggcgaggt gcggtcggcc gaagcgctcg acatgctcca ggccatgaac acgggccatg
     4561 acggctcgat gtccaccggc cacgccaact caccgcgcga catgctggcc cgtctcgaga
     4621 ccatggtcct catggccggt atggaccttc cgctgcgcgc gatccgcgag cagacggcct
     4681 cggcggtgga cctcatcgtc caccagaacc ggctgaagga cggtacccgc aagatcgtga
     4741 gcatcaccga ggtccagggc atggaaggcg atgtgatcgt catgcaggac gtgttcacct
     4801 tcgagcagac cggcgtcgtg gaagggaaga tcgaaggtcg actcaagccg accggcatcc
     4861 ggcccaagtt cgtcgagaag ttcgagacgg ccggaatcca tctgccggcg aacatctttg
     4921 gttcaccgtt ctaggttgag cgcaggagca gccctcgcat gaaccttctc ccgatcatca
     4981 tcgccggtct cggggccatg gccatcgtgt tcgtgaccct cggcatcgcc atgagcggtg
     5041 gcggggggac ggttaccgat cgcctcgagc gctatgcgtc gtcgcgtggc ggccgctccg
     5101 acaaggacga cgatcccgaa cgcgagtcgg cggtcatcgc cggcctgtcg aaggtgatcg
     5161 aacgccagga catgaccgcc aggctcagca ccgatctggc ccgcgcggac ctcaagctca
     5221 agccggccga gttcatcctg atctggatcg cgactccgtt cgcgctcctg ttcctcgggg
     5281 ccgtcctggg cttgaccctg atcccggcgc tccagacccc gctggccctg gtggccttgt
     5341 tcgcagtcgg gttctggctg cctcgcttct acctcggccg tcgccagcgt ggccggctgc
     5401 aggccttcaa cgaccagctg ccggacacga tcaccctgct cgcgaactcc ctccgcgcgg
     5461 gatcgtcatt cctccagggc atggagctcg tgacccgcga ggcccgaccg ccgattgccg
     5521 aagagttcgg acgcgtcgtc cgcgagatga gcctgggcct ggcacttcag ccggccctcg
     5581 ccaacctcgt tcgccgggtc gcctccgagg acctcgagct gatggtcacg gccatcaaca
     5641 tccaggccca ggtcggtggc aacctggcca ccgtcctcga cgcaatcgcg ttcacgatcc
     5701 gtgagcgcgt ccgaatccag ggcgagattc ggactctgac cgccatgcag cgatacagcg
     5761 gctacgtcat cgttctgctc ccggtcgggc tcgccgcgct cctgttcgtc atcagcccca
     5821 cctacatgac cccgatgctc gagcggcctc ccgagatgct cggtctgccg atgggcgtcg
     5881 tcctgcttct cctgggcctg ctgagcatgg gcatcggctg gctcttcatt cgccgcatcg
     5941 tcgatatcaa ggtctgatct gatgttcgag aacccactcc tcgtcctcat cgcgggtctc
     6001 gcggccggcg ccgtcctgtt catcatcttc ggggccgtat cgaggccacc gaaggacgcc
     6061 gtccagcagc gcctcgagca gctcgtcgtc aagcccaaga ccctcgagga agcggagctc
     6121 gagctcccgt tcttcgagcg ggccgtccgg cctgtggttc agcgtgtgtc caagatgggc
     6181 cggcggggcg gcggggtcaa catgatcgag cgcgccgagc agcgcctcca cgcggccggg
     6241 tatcccggcg gcctccgtgg agcggactgg atgggcgtca aggtgctcgc cgcgatcgtg
     6301 ttcgccatcg cgttcacgat catcttcctg ggcgtctcgg gattcccgac cggtatcctg
     6361 ttcggcctcg gcggattggc cgtgggcttc ctcgccccgg agttctggct gggccgcaag
     6421 atccgcgggc gacagatgga gatgatcctc cagctgcccg atgcgcttga cctgctcacg
     6481 atttcggtgg aggccgggct tggcttcgac gcggcattgg ccaaggtcgt cgagaagatg
     6541 gatggaccgc tggtggcgga gttccgccag gcgctggccg agatccggat gggtcgtctg
     6601 cggcgcgagg cgcttcgaga cgtcgccacc cgcgccgacg tgcagccggt gaccaacttc
     6661 atcggggcga tcgtccaggc cgagcagctc ggtgtgccga tcgccaaggt gctccagatc
     6721 cagtcccagc agctgcgcat cgagcgccgc cagcgagcgg aggaagcggc cgccaaggcg
     6781 ccggtcaaga tgctgttccc gatggtggga tgcatcttcc cgaccatctt catcgtcatt
     6841 ctcggacccg ccatcgtcac cgtcgtccga ggcgtcggga tctgacgtgc agccggcgct
     6901 gcggccggcg atcctgctgg tcgacgacga cgctaccctg ctctcggtcc tctcgcgcca
     6961 cctgacccgc gaagggttcg cggtgcgaac cgcgtccgga ggtcaggccg ccctggcctc
     7021 gctcagccag tcatggccgg ccatggtggt tgtcgacctc atgatgcccg gcatggatgg
     7081 cttcgagctc agccagcgga tcaagcggac cgccgacctc ccgatcatcg tcctgagcgc
     7141 ggtagatgcc gacgagacga agatcgacgc cctccagcat tttgcagagg actacgtcgt
     7201 caagcccttc agccccgacg agctggtcgc ccgcatccac cgggtcctcc gtcgggcggc
     7261 cccgagccgc acgcaggtgt ccatcggtga cggcgacctc gaaatcgacc tcgccgggcg
     7321 ccgcgtggcg accccggctg ggtcgcaccc gctcacgccg accgaggtgc gctttgtcca
     7381 ggtgctcctc ggctcgctgg atcgcaccgt cccgaccgag acgctgctcg accgggtgtg
     7441 gtccgaagcg gacggcgcgg acgcgtccta cgtgtgggtc accgtccgcc gcctgcgtcg
     7501 gaagctcgag ctcgacccgg accgcccaag attcctgctg accgagcgtg ggatcgggta
     7561 tcggctggct tcaggctagc gccgccgccg aaggtacgcc cggggtacca cccgccaggc
     7621 agacggctgc aaggtgcctc gcaaggtgcc gttggtagat ttcgcaccca tgttccagaa
     7681 gtggcgtcag ttgcccaggc gtacctccgc tcgccggtac cttccggtga gggcttttgc
     7741 gcgcggcaac cccgatcctg tctcggtgcg acgtcatgat tccgaacgcg ggcagaccct
     7801 ggtcgagttc gccctcgtct tccccatctt catcctgctc atcgttggcc tgatcgagtt
     7861 ctccctcgcg ttcaacgcgc tcctgagcgt gaacttcgcc agccgcgatg ccgctctgct
     7921 ggccgccgag gccggggagg actccggcgc ggactgcgtc gtcctggcca gcattgacga
     7981 agacgtgcag ccgccggcat cgcgcgtgcg gatctccgag gtccgcatct actgggcgga
     8041 cagccaggga aacgaactcg acgcgcaggt ctaccgccgc aacagcggag cgcccaccaa
     8101 ctgcaacgtg cccggcctcc cggcgacctt gccgtacacc ctgcaggggg ggagcagcta
     8161 cccggagaac gagcgctgct cggtcctggc aggctgtccc gaggatccgg ggcacaccga
     8221 gctcgacaac atcggcgtct cgatcaccta ctcctacagc tgggtcaccc cgatggcgaa
     8281 gatcatcagc ctcggcggga cggggttcac cctgacccat gccaacgtca tgcgcatgga
     8341 gccggtgctg tgagcgccct ccgacgactc cacgccaggt tccgcagcgg cgccgagcga
     8401 ggccaggcca tgctcgagat ggccctcatc ctgccggtcc tgctcctgat cgtgctcggc
     8461 accctcgagt tcgggttcgt gatggaccac aagctgaccc tcgagtacgc gagccgcgag
     8521 gcggcgcgcg tcggctcggc actcgccgac ggcggcgggt cccccgggtg cctcaccgtg
     8581 gacaacgaga tcatcgccgc cgcccagcgc atcctggaga gcagcgactc gaacatcgag
     8641 attgccgccg tcagccagat ccggatctac cgcgccgacc tcgggccagg ccccgacgga
     8701 cgcgaggacg tcggcagcga cccggtcaac gtctggctct accagccggg cgccggcccg
     8761 accgtggatg gcgtgccgct cgacttcgcc cccaccggag cccccggctg gaatgcgtgc
     8821 ggacggatca acaccggcgt gaaccccgaa tccatcggcg tcagcgtgac ctacacctac
     8881 gtctcccgga caccgctgca ggcaatcatg aatctgctcc agatcgggat gtacgacttc
     8941 accgtcttcg aactcaaccc caccaacaac tgaggctcga ccgtgcccac gattcgtacg
     9001 cagtccatca ccgacgaagc gcagccccgc atgacctcgc gcggcaaccg cccgcgcggg
     9061 caggtcctgg tgctgttcgc cctgctcctg accttcctgt tgggcacggc cgcgttcgtc
     9121 gtggacctgg cctggatctg ggtcaacgag ctgaaggtcc agcgcgccgc ggacgcggcc
     9181 gccctggccg gggtcgtctt cctccccggc aacccggtct cggccggcac ctcggcgcac
     9241 gaggagtcgc ggaagaacgg gtacctggac ggagacggca ccatcaccgc agccccgaac
     9301 ggcggcgtcg atgtctgggc acgaccggat tcgctggatc ctcgccggat gctggtcacc
     9361 gtctcggcgc cgatcgagac gttcttcatg aagatcttcg gtttcgacca actgcaggtg
     9421 acccgtgatg caaaggccga gttcatcctc cctgtgccca tgggcagccc ggagaactac
     9481 tacggcgtct tcggcgcgct gcgtaccccg gacggcggcg tctggcagcc aaatgccagc
     9541 accacgctcg gcccgctgac gccgcgcaac gtgctctcgt cgagcacggc cgctcccggc
     9601 acgaaccaga acaacaacgc cgacagctgc aacacgaacg cggcggcctc gtggggcatc
     9661 ttccccacga ccggcgcggc gccacagcag gacgaccggg tcaattcgtc caacaaccag
     9721 tggatgcggt cgccccggat caacgaccgc gtgtgcttga acgactttgg cttcaccttc
     9781 cccgcgaacc cggctactcc tcccggagcg ccggcgtacc cgggccacgt ggtcgacggc
     9841 atcgaggttc gcctcgagac cttcgcctcc gatacgagcg gctgcgagct ccgggttgac
     9901 ctcatgtggg gcgcggccgg ctcgggtggc acgaacaaca ccaacacgac cgggaccggc
     9961 gacaagatcc ttgccgtcca ggggacggag gcaacgggcg cgccctatac cgtcctgggc
    10021 ggcgccagcg acacctggac acgggcatgg cccctgtccg acatcaccgc caacaccttc
    10081 cgcgtccgcg tgtggaacac ggatccggcc gcctgcagca cgctgaccgc cggccagccc
    10141 gcggccggca tccggacccc gaacagtcct cccgcaaccg ctccatcggc tccggtcgct
    10201 ggcgaggtat acgtggacct ggtccaggtc gtgatccact accacaccta cacctggatc
    10261 cccgacccgc aggtccgcag cccgtacaac caggcgctga acgcgcgtgg cctgtggggc
    10321 acgttcatca accagggcgc cgagaaggtg aacggcgacg cgtacctccc caagtgggat
    10381 ccgcggacgt cgggaacgaa cgcggagtac aacccgaacc agtactacaa ctacgcgatc
    10441 gagatgccgg caggagccac ggctggcgag gtctggatct tcgacccggc gatgtgcgcc
    10501 accgacacgg gcggccagtt cggcaccggt gaccgcttct tcggcggctc gaccgcggct
    10561 accagcgcct actacacgct gtggaacaca ttcaacacgc cgttcgacct cggcgatgac
    10621 acgttggcga acccggtctc gcgggcgcac aacatcttcg ccaaccagca agccgacgac
    10681 gacaccctgt tcggcagcgg ctcgggccct gtcggcacct cgtgtaccca gggagcgacc
    10741 gccgccctga acgacggacg cttctggcac aaccgctggt acccgatgca gccgtacgtg
    10801 accggagcgg cagccgagga gacgcctgcc tccgcggagc agccgtggcc gctgctcggc
    10861 ggcggaccga cgtcagcgcc gacgctgaac atgaccggtg gcaacacgta ccggctccac
    10921 acccgcacaa cgaacccgac caacccgacc gccatggaca acgcgaacgg gcacaactcg
    10981 ttctcgatct ggacacaggc gtggtgcggt gggagcgtct gtgcagccgc accgcgggtc
    11041 tacggcctgg gcgcgatgga ggccttcacc ccgctgcccg gtggtggcgc ggcccagttc
    11101 tacctcgccg agatcgagcc gatccatgcc ggcaagagcc tcgagatccg gctctgggac
    11161 ccgggtgaca ccggcagcct ttcggccgat ctgcggatca agtacccgtc cggcgggtcg
    11221 taccacaacg ccgacatgga cttcatcgcc aacgcggtgg cgtccggggc ggcagcgtgc
    11281 aacggtcgga gcgacgacac ggtgggtcca cctggcccgc ttgatgacgt catcacgacg
    11341 aacaccggag ggagctcgca gtacaacggg tgctggctga cgatcgtggt cgtcatcccc
    11401 gacacctatg acggcgaccc ctgccccggc gtgcccccgt gcctcgacca ggactggtgg
    11461 aagatcgagt acacgatggg cggcgccgcg acggacaacg cgttcgacct gaccacctgg
    11521 caggtgaccc tgcgcggaaa cccggtccac ctggtggtgc cgtaaccggg tagcgagccg
    11581 cccgcaaggc tcatcggtct ctggggaggc cctcgagagt ccgtatcatg cgaaccatga
    11641 acggcgtcgg gggcctcgcc gcgtcccgga ccggctcgcc ccgctgagga acgggtggat
    11701 ctcggtcagc ccgcgctccg cctgttcgcc gtcattgcgg tcgtcgccct gccggccctg
    11761 gccgccctcg cgctggtcct cgcatttgcc gaggggtggg tcgccgaggt gggcatcgga
    11821 accgcgttcc tgatcgtcgg cctggggaca atggcgtggg ccgggatcgt ggcggcctgg
    11881 acagcacggg ccgtctcccg cgacctccag gacgtggtcg cgatcgcgac ccggggcgat
    11941 gcgtcggacg cggtcggcgg cggtgacgag gagcttcgca ccgcccagcg acagctgcgc
    12001 ggtgcgctgg acgagcggaa ccggcagatc gcgacccttg cggccgacgt ggccgccacc
    12061 ccgatcaccg gtggcccggt ggaggtcgcc gcgggcgttg tgaccgtcgc ccgccaggtc
    12121 gcgggtgacc cgacatggct gctcgtcgtc ctccactcga gcgaccccgc ggcgctgccg
    12181 gccggggtct acgacgacga cccggccacc cccacccggt cggtggacga gctcgagcag
    12241 tgggccgccg tcaccggcga ccccgcgcgg ctgcagccgc gccatctcat cggtccatgg
    12301 ggagccgtgg tggtagtcga catctcgagc ggtgacgagc tcaccgggtt cctcctcgcg
    12361 ccgtgggagg gccggccgga gccttccccg gccgagcgcg acctgctgag cctcatcgcc
    12421 caggtcgccg cctcgaccat cgagcactcg ctgctctatg cgcggctgcg cgcccagacc
    12481 gacgacctca accggctcgc cgccgtgcag agcgattttc tgcgagggat cacccacgac
    12541 ctgcagacgc ccctgaccag cattcgcgcc cttgcggccg acatcggcca ggaggcggag
    12601 ctcaacgccc aggcccgggc cgacctggag gccatcgccc accaggcgga tcggctccgg
    12661 cgaatggtcg gacagctgct ggccgtgtca cgcctggacg caggggccct tgagcctcgg
    12721 caggaggtct tccgggtgga gccgatcgtc gagcgcacgt gggaggcgct ccgcgccgcc
    12781 gagcggccgt tcacgctgga aaccgatgga tcgacccagc tggcggtcgg cgaccctgat
    12841 cggctcgagc aggtgctgtg ggcggtgctc gacaacgcgg tgaagtactc gccaccacgg
    12901 tcggcgatca ccgttcgggt cagcgccacc tcgggaccag ccgggatgca ggatggctcg
    12961 ggcgggcacg ggctcgagat cgcggtggtt gatcgaggcc gtggcatgac ggccgcgatc
    13021 cgcgatcagg ccttcgacca gttcttccgc gacgacgagg ctcgtcgggc ggcgccggac
    13081 gggagcggga tcgggctgta cgccgcccgg ggcctgatcg aggcgatggg cgggtcgatc
    13141 tcgctcgatg gcggcgacga ggaagggacg gtggtccgca tccggctgcc ggcggagccc
    13201 atctcggctg acgagagctg accgatggcc acgacctggg tccgtgtcac gaatccgacg
    13261 cggggcacca ccctggccga gcattgccgg atggcgacct cgatgcgcga ccggacggtt
    13321 ggactgctcg ggacctcggc gctcggcccg ggcgatggcc tgtggatcga gcggtcgccg
    13381 tccatccaca tgttcttcat gcgctacgcc atcgatgcgg tcttcgtcga cggcgacgac
    13441 cgcgtggttc ggatcgtcga gcgccttcga ccgtggcgga tcgtggcctg ggcacgcggg
    13501 gcgaaggact gcatcgagct gccggccggg tcggtgcagg ggaccgcgac cgccgtcggc
    13561 gacgtgctcc gtcgcgagcg gggcgaagcg cccgtctcgg aggcccgcta agcccgcggt
    13621 aatggcccgc acgcggccga gcaccagact gggccaatgg gcctgctggg cgtcctcctg
    13681 ccgccgtcct gcgcgggatg cggccgatac ggcgagccga tctgcgccga ctgtcggtcg
    13741 tgcctgcgac cgctgtccaa cggatcggac cagttcctgg cgccggaccc ggggatcgtg
    13801 gtcggcgagg cgctcctact cgcggcggcc gcgttcgccc atcgggacga ggcgcagcgc
    13861 atcctccgcc ggctcaagta cggcggcggc cggaggctgg cggcaccgct cgcccacgag
    13921 gcctggcccg cgttccgccg tctcctcgcg atcaccggtc ccgtgcccct cgtccccgtt
    13981 cccctgcacc gcagccgaca acgcgaccgc ggctacaacc aggcggagct gatcgcccgc
    14041 gagctcggaa gacgggcacg agcgccggtc tggcccgcga tctgccgtca gcgccgaacc
    14101 gagcgccagc atggcctcga tcgggccacg cgcatgcgga acctgcgcga tgcgatggcg
    14161 ttcgagcctc ccccgtcgtc cggcgcgcgc cctcccacca cggtgatcct ggtggacgac
    14221 atcctgacca cgggcgccac gttcgaggcc tgcgccgtca ttctgcggga ggccggggtg
    14281 gccgaggtct acgggttcgc gatcgcgcga gaggtgtgat gggcgcggtt tgcgaaggca
    14341 ggtggtaccg taatcgggac cccgcgaacg ggagagaccg gccgtgcaga ccatcctcca
    14401 cgcccgtcac ctcgagctcg atgcccggct gcgggcccgg atcgagcgca agctccagcg
    14461 ccttgatcgc ctcgctgatc catcagccct ggctacggtc gaactgaccg cacgggcttc
    14521 ccgagcctcg gatacggcac ttgtcgccgc cgtgaccctg gtcagcggcg gcacgacgct
    14581 ccactcctcg gcggcgggag ccacgcccat tgccgcggtg gacgccgtgc tggacaagct
    14641 cgagcggcag gtcgtgcgcg ccaagcaacg gctgcgggag agccggcggg cgcccgtggc
    14701 gaaggcagct gcagcgcgtg ccgcgacaac atcgatgtca tccgacggcc ctgccgagac
    14761 ggacgtggtc gagatcaccc gcctggacat ggttcccatg tttcccgagg acgcgatcag
    14821 ccggatggag gagctggacc aggcgttctt cgtgttcctg aatgcgcaga ctcgacgggt
    14881 gagcgtcgcc taccgccggt cgaacggggg ccacgggctg atcgacccga tcgtctcgcg
    14941 ttgatgcggc accctgtcga cgcgcggcgc ctcagccgag gacgagggga agcaggagga
    15001 ggacagccac gatgacgatc agggcaacga agaagaccag cagcccgatg gtcagcagat
    15061 ccagcccgga ccggtcaggg gtgtccacga gcgcacgcta gcagaccacc ctttcctgcg
    15121 ggattgcgct aggcgccgct ccgcatcttg gagttcggag gcgtatcccc attcgaggcc
    15181 ggctgcgttc cgccattggc catccccggt cgcaggcggc gagaacgaac cgagatgtac
    15241 gcctgcatga gctcgcggac gtcggcctcg gtcaggagct cgtcgccgcg aagcgcgtat
    15301 tccacccggc tagtgggcaa gagctgacgg gtggccaggc cggcgaaata ggtcggtccc
    15361 tcggactcgt cgtggatctc gcgaaggccg cgggcgagct tcgtggcggt gccaagcgag
    15421 ggcatccggt cacccttgat cagccgggag atggtcgaat gatcgacccc cgactggagc
    15481 gccagttgac gctggctcat cttcttctcc ttcagctggc tgcgaagcca gctgttgaag
    15541 gatcgcggtg tctggagcgt catccacgag gctcgtacgg cgcgtccgcc cgcggatgcg
    15601 ggtggtgcgt gcgcgcaccc gaagcattgc gttcaggcgc cgttggctca atgggacgaa
    15661 ggtacttcgg cctgggctcc ggttcgggac gcgagctcag gtttcagtga tcggctgctt
    15721 ggccggaggc ggtgggttgc caaggcccgc gctgtgcgcc cggcgcacga gcgggctccg
    15781 gacgtagtcg agaccgccga gcacgatcac ggtcaggatc gagatcgcgc gatcggtcaa
    15841 cgcgatggcc accgccgact ccgggctgac cccgtacagg gtgagcgccc ccacgatccc
    15901 cgcttcgacg aagccgatcc cggccggcgt gagcgggatc gccgtcagca gagccgccac
    15961 gagggccacg aacaccgccg ccgagatgcc cagctgggcg tcgggaaggt cgagcgcctt
    16021 gatcacgaag aacacgcgaa gcccttcggc cagccacacg acgacggtca gcgagccgat
    16081 caccaccacc gcgcgtggcg tgagcgccgc gatcgtccct tcgcggaacc gggcgtagaa
    16141 cccctcaacg cggttcggca gaaatcgctc gatgtgctgt cccgcgaacc ggaggaccag
    16201 cagcagcacg accaggatgg tcgccagcag ggcggcggcc acgaagatga agtcgacctg
    16261 tggcatggcc cggccgcgga agctccagta cccggcggcg acggccagcc cgaagatgac
    16321 cacgatgtcc gcgatccgct cgatgaagat ggtccccacc gtccgcgacg cggagccgcc
    16381 gtagttgccc ttcagcagga aagcgcggta gaggtcaccg agcttggccg gcaccaggca
    16441 gttgacg
//
LOCUS       DASYGU010000259         5785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1533538, whole genome shotgun sequence.
ACCESSION   DASYGU010000259 DASYGU010000000
VERSION     DASYGU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533538"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VGO32_07420"
     CDS             <1..1510
                     /locus_tag="VGO32_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200618.1"
                     /translation="SLEATGRARCSLQGGPALPIVAKRYDAPPGPGGGFVDAVATAAT
                     SSTGAVDATNVLGYGGRTPATEANPGPVVELYGPGAKANNDSSFRGFVALDVRNFQSV
                     FSRVYYNGVTAGTNANTLKNMQGDYILTGYPGPGFPSITSPADPNDQVAVMSGNSSAM
                     VLGNFSQVYEPGDRVMLAVYNGTVMEIPDFAITPPSFISLASTTSSPVTGPTFTVSRN
                     ASFNSTVTLHLHGDHLAPNPAHDLIPGEPPTNAAPTAGHMNQPVWSTNVFIPSAGGTN
                     VVMQSISTNAVPAGIYTVWLEGHSGNPYFQTRRQPTAVRIGGAVRDFSLTNSIAYGDI
                     PSLGGSTNLSLYVSTTSSSSTRWGPTGTAVSLSVDAGTFTDCSYGVASINPGQLTISP
                     ASVTPSSSGNGAAATLTINSLGLAPGCYAFTVRGTGTNGAGQPVTHLLQVTFTVATSS
                     GSGQYVDIIGFTVFDITNADSNSISGRAVSPIAADPNDPVLRRAQRARLVPW"
     gene            1532..2371
                     /locus_tag="VGO32_07425"
     CDS             1532..2371
                     /locus_tag="VGO32_07425"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEV7200619.1"
                     /translation="MEYEYTDRHRRRSTLYIVVGVFAALVVGAIVLVTLQASPLLAEP
                     VEMRVVVVAAQDIAARKPIEEGDVVTRSVAADPTNDSAYTLIEDVLGRVSSVPILAGQ
                     LVTRNALASTSAGQSYAILDAGETFDPNGPDLRAVSVSVPDDRAVAGTLTPGQRVDLL
                     VTLAINPEIGVVVEEPAASAEPAAGEAAPFIAGPSTKVTLQMLTVLARNGSIYILRTD
                     LATAERIAELAAAGGQFTLALRADEDVRTAETTGSTLDMLLAEFGFPIPEPPNLEGQG
                     AGN"
     gene            complement(2436..3011)
                     /gene="coaE"
                     /locus_tag="VGO32_07430"
     CDS             complement(2436..3011)
                     /gene="coaE"
                     /locus_tag="VGO32_07430"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958992.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV7200620.1"
                     /translation="MKVIGLTGRIGCGKSTVAAMLHERGAATIDADEVAREVRETDPN
                     AGAAIHARFGTDEPAALARLVFGDPQALADLEAIVHPAVRDRVRDRLAALEAAETAVA
                     VVEAIKLLESPLRERCDEIWVVVCRPEDALDRVAQRGMSAADAERRLANQRSAEEMVA
                     AADVVIDGSAALRQTQDQVHAALSRLLENEP"
     gene            complement(3008..3862)
                     /gene="mutM"
                     /locus_tag="VGO32_07435"
     CDS             complement(3008..3862)
                     /gene="mutM"
                     /locus_tag="VGO32_07435"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEV7200621.1"
                     /translation="MPELPEVETVARRLQTRLPGAEISGAEVLWGRTIAFPQPPERFA
                     IEIVGGVVERVGRRAKSVVLYLDDGRVLTVALRMSGALIVAAPGAAPDPHARVVLELA
                     DGRQLRFRDARKFGRIGLYPGRGRRRVADVFSKHGPEPLAPSFTAARLAARLRRRSAR
                     LKTLLLDQTFIAGVGNIYADEALWRARLHPLRRADTLTEVEIARLHRAIRASLREGLA
                     GGGASYRDYVDPDGEPGLAAERMRVYRRTGEPCFRCGHRIERIVVNTRATHFCPHCQP
                     APPMVAGR"
     gene            complement(3855..>5785)
                     /gene="polA"
                     /locus_tag="VGO32_07440"
     CDS             complement(3855..>5785)
                     /gene="polA"
                     /locus_tag="VGO32_07440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV7200622.1"
                     /translation="PMTETGETPVVHERRRSAASLPDPRSDPKGAIEQVDPRLVADDA
                     DRLELEAWLAAHGDEVGIGVAMGSGRLVDRPLLGLALAGADGTSWYLRAGGDEPLPRW
                     FLRDDRPLIGHDLKPLHTKLLQRGMAVSGENRDTLVASYMVNPALRAQSIEDLAAQRF
                     GAEVPPRAVTPESGGEEPVVARRAAGEALLALLALPALESDLADIGLTNLFHEVEMPL
                     VPVLARMEAAGVLIDRDALNAMSEEFADQLDGLERRITELVGHAFNIGSPRQLETVLF
                     DELGLPSAKRTKTARSTDASVLEELKDKHEVVGLILEHRQIAKLKSTYADALPGLVDP
                     EGRLHTTYSQAVAATGRLSSTDPNLQNIPIRTALGRRIRRVFVAPPGKLLLAADYSQV
                     ELRILAHVSGDPGLTAAFAAGEDIHRSTAARVLGIAPEDVTPEQRSMAKMVNFGVAYG
                     MSDFGLAERLHIDRETAGTFISDYFAAYEGIRRYTVEIRILARDQGYVTTLLGRRRYL
                     PELTARNFSLRAAGERMAINMPIQGTAADGIKIAMVRLDAAMQERGLRSRMLLQVHDE
                     LVFETDADERDELATLASTTMEAALPLDVPLTVDLKVGTDWDRMDRLVPADGGYRVLE
                     VDHAALAEEDPAVVLADA"
BASE COUNT          878 a         2108 c         1960 g          839 t
ORIGIN      
        1 cagcctcgag gccacggggc gcgcgcgctg cagcctgcag ggtggcccgg ccctcccgat
       61 cgtggccaaa cgctacgacg ccccgccggg gccgggtggc ggattcgtcg atgccgttgc
      121 caccgcggcg acgagtagca ccggggcggt ggatgcgacg aacgtcctgg gctacggcgg
      181 ccggaccccc gccaccgagg ccaatcccgg tcccgtcgtc gagctctatg gaccgggcgc
      241 caaggccaac aacgacagct cgttccgcgg gttcgtggcc ctcgacgttc gcaacttcca
      301 gtcggtgttc tcgcgggtct actacaacgg ggtcaccgcg ggcacgaacg caaacaccct
      361 gaagaacatg cagggcgact acatcctcac cggctatccc ggtccgggat tcccgtcgat
      421 cacctcgccc gcggacccga acgaccaggt cgccgtgatg tcgggcaaca gctcggccat
      481 ggtgctcggg aacttcagcc aggtctacga gccgggcgat cgggtcatgc tcgcggtcta
      541 caacggcacg gtgatggaga tcccggactt cgccattacc ccaccgagct tcatcagcct
      601 ggcctcgacc acgagctcgc cggtcacggg gcccacgttc acggtctcgc gcaacgcgtc
      661 gttcaacagc acggtcacgc tccatctcca cggggatcac ctggcgccga accccgccca
      721 cgacctgatc cccggtgagc cgccgaccaa cgcggcgccg acggcggggc acatgaacca
      781 gcccgtgtgg tccacgaacg tgttcatccc cagcgccggc ggcacgaacg tggtcatgca
      841 gtcgatctcc accaacgccg ttccggccgg gatctacacg gtctggctgg aaggccattc
      901 gggcaatccg tacttccaga cccgccgtca gcccaccgcc gttcggatcg gtggcgccgt
      961 ccgcgacttc agcctcacga attcgatcgc ctacggcgac atcccaagcc tgggaggctc
     1021 gaccaacctc tcgctctacg tctcgaccac gagctcgagc agcacccgat ggggacccac
     1081 tgggaccgcg gtcagcctga gcgtggatgc cggaacgttc accgactgca gctacggcgt
     1141 cgccagcatc aatcccggcc agctgacgat ctcgccggcg agcgtgacgc cttcctcgag
     1201 cggaaacggg gcggccgcga cgctgaccat caactcgctc ggccttgccc cgggctgcta
     1261 cgccttcacc gtccggggga ccgggaccaa cggcgccggc cagccggtca cccacctgct
     1321 ccaggtcacg ttcacggtgg ccacgtcctc gggcagcggt cagtacgtgg acatcatcgg
     1381 cttcaccgtt ttcgacatca ccaatgccga ttcgaactcg atcagcggcc gggccgtcag
     1441 tccgatcgcc gctgacccga acgaccctgt gctgcgccga gcgcagcgcg cccgcctcgt
     1501 cccctggtaa cccgcatcaa ggagcctcgt cgtggaatat gagtacaccg accgtcatcg
     1561 tcgacggtca accctctaca tcgtcgtcgg cgtgttcgcg gccctcgtcg tgggcgcgat
     1621 cgtcctggtc acactgcagg ccagccccct cctcgccgag ccggtcgaga tgcgggtggt
     1681 ggtcgttgcc gctcaagaca tcgccgcccg caagccgatc gaggaaggag acgtcgtcac
     1741 ccggagcgtg gccgccgatc cgaccaacga ctccgcctac accctcatcg aggatgtcct
     1801 gggacgggtc agcagcgttc cgatcctggc tggccagctg gtgactcgca acgctctggc
     1861 ctcgacctcg gcgggacagt cctacgcgat cctcgacgcc ggggagacgt tcgacccgaa
     1921 cgggcccgac ctgcgcgcgg tgtccgtcag cgtgccggac gaccgggcgg tggcgggcac
     1981 ccttacaccg ggccagcggg tcgacctgct cgtgaccctg gcgatcaacc ccgagatcgg
     2041 ggtcgtggtc gaggagccag cggccagtgc agagcccgcc gcgggcgagg cggctccgtt
     2101 catcgccggt ccgtcgacca aggtgacgct gcagatgctc actgtgctcg cccggaacgg
     2161 gtccatctac atcctccgaa ccgatctggc cactgccgag cggattgccg agctcgctgc
     2221 cgcgggaggc cagttcacgc tggccctccg cgccgacgag gacgttcgga cggccgagac
     2281 gacgggatcc accctggaca tgctgctcgc cgagtttggc ttcccgatcc cggagccccc
     2341 gaacctggag ggccagggag cgggcaactg atcagccgcg acgaggcggt gagggccccg
     2401 gcgtcgccac gggcggcgcc ggggctcggt tctagctagg gctcgttctc caacagccgc
     2461 gacagcgcgg cgtgcacctg gtcctgggtc tggcgcaacg cggcagatcc atcgatcacg
     2521 acgtcggccg cggcgaccat ctcttccgcg cttcgctggt tcgccagccg gcgctcggca
     2581 tcagccgcgg acatgccccg ttgcgcgacg cggtcgagag cgtcctccgg tcggcagacc
     2641 acgacccaga tctcgtcaca gcgctcgcgg agcggtgact cgagcagctt gatcgcctcc
     2701 accaccgcga ctgcggtctc agccgcctcg agggcggcga gccggtcccg aacccggtcg
     2761 cggaccgccg ggtggacaat ggcctccagg tccgcgagcg cctgcggatc tccaaacacc
     2821 agccgggcga gggccgcggg ctcatcggtc ccaaaccggg cgtggatcgc cgcgccggcg
     2881 ttcggatcgg tctcacggac ttcgcgcgcc acctcgtcgg cgtcgatcgt ggccgcaccc
     2941 cgttcgtgga gcatcgccgc cacggtgctc ttcccgcacc cgatccggcc ggtcaggccg
     3001 atgaccttca tcgcccggcc accatcggcg gcgccggctg gcaatgcggg cagaagtggg
     3061 tcgcccgggt gttgaccacg atccgctcga ttcggtggcc gcagcggaag cacggctcgc
     3121 cggtccgccg gtagacccgc atccgctcag cggcgaggcc gggctcgccg tccggatcga
     3181 cgtagtcgcg gtacgacgcg ccgccaccgg ccagtccctc gcgcagcgac gcgcggatgg
     3241 cgcgatgcag ccgcgcgatc tccacctcgg tcagggtgtc ggctcggcgc agcgggtgga
     3301 gccgtgctcg ccacaacgcc tcgtcggcgt agatgttgcc caccccggcg atgaacgtct
     3361 ggtccaggag cagggtcttc agccgggcgc tccgtcgccg gagtcgggcc gcgaggcgtg
     3421 cggcagtgaa cgacggggcg agcggctccg gaccgtgctt cgagaacacg tccgccaccc
     3481 ggcgtcggcc gcgccccgga tagagaccaa tccgcccgaa cttgcgggca tcccgaaagc
     3541 ggagctggcg gccgtcggcg agctcgagca cgacccgggc gtgcgggtcc ggcgcggccc
     3601 ccggggcggc cacgatcagc gcgccgctca tccggagcgc caccgtcaag acacgaccat
     3661 cgtcgaggta caggacgacc gacttcgcgc gccggccgac ccgctccacc accccaccca
     3721 cgatttcgat cgcgaaccgt tcggggggct ggggaaaggc gatcgtccga ccccagagca
     3781 cctccgcccc gctgatctcg gcacccggca gccgggtctg gagccgccgg gcgacggtct
     3841 cgacctcggg cagctcaggc atcggccagg accacagccg gatcctcctc ggccagcgcc
     3901 gcgtgatcga cctcaagcac gcggtagccg ccatcggcgg gcaccagccg gtccattcga
     3961 tcccaatcgg tcccgacctt gaggtcaacg gtgagcggga cgtcgagcgg tagcgcggct
     4021 tccatcgtgg tcgaggccag ggtggcgagc tcgtcccgct cgtcggcatc ggtctcgaag
     4081 acgagctcgt cgtggacctg gagcagcatc cgggaccgca ggccgcgctc ctgcatggcg
     4141 gcgtccagcc ggaccatcgc gatcttgatg ccatccgccg ccgtgccctg gatgggcatg
     4201 ttgatcgcca tccgctcccc cgccgcgcgg agcgagaagt tccgggcggt gagctcgggc
     4261 aggtagcggc ggcgaccgag gagggtggtc acgtacccct ggtcccgggc caggatcctg
     4321 atctcgaccg tgtagcgacg gatcccctcg taggcggcga agtagtcgct gatgaacgtg
     4381 ccggcggttt ctcgatcgat gtggagccgc tcggccaggc cgaagtcgct catcccgtag
     4441 gcgaccccga agttcaccat cttggccatc gagcgctgtt cgggagtcac gtcctcgggc
     4501 gcgatgccca ggacacgggc cgccgtggac cgatggatgt cctcgccggc ggcaaacgcg
     4561 gcggtcagcc ccgggtcgcc cgagacgtgg gcgaggatcc ggagctcgac ctgcgagtag
     4621 tcggcggcca gcaggagctt cccgggcggg gccacgaaca cccggcggat ccgccggccg
     4681 agggcggtcc ggatcgggat gttctggagg ttcggatcgg tgctcgacag ccgaccggtg
     4741 gcggcgacgg cctgcgaata ggtggtgtgc agccggcctt cggggtccac caagccgggc
     4801 agcgcatcgg cgtaggtgga cttcagcttg gcgatctggc gatgctcgag gatgaggccc
     4861 acgacctcgt gcttgtcctt cagctcctcg aggaccgagg cgtcggtcga gcgcgccgtc
     4921 ttcgtccgct tcgcggaagg caggccaagc tcgtcgaaca gcacggtctc gagctggcga
     4981 ggcgaaccga tgttgaaggc gtggcccacc agctcggtga tccgccgctc gaggccatcc
     5041 agctggtccg cgaactcctc ggacatcgcg ttcagcgcat cgcggtcgat caggacgccg
     5101 gcggcctcca tccgggccag gaccgggacg aggggcatct cgacctcgtg gaacaggttg
     5161 gtcagaccga tgtcggccag gtccgattcg agggccggca gcgcgagcag ggcgaggagc
     5221 gcctcgcctg ccgcgcggcg ggcaaccacc ggctcctcgc cgcccgactc gggggtcacc
     5281 gcccgcggcg ggacctcggc cccgaagcgc tgggcggcaa ggtcctcgat cgactgcgcg
     5341 cgcagcgccg gattgaccat gtacgaggcg accagcgtgt cgcggttctc gccggacacg
     5401 gccatcccgc gctggagaag cttggtgtga agcggcttca gatcgtgacc gatgagcggg
     5461 cggtcatccc gcaagaacca ccgcgggagg ggttcgtcgc cgcccgcccg caggtaccag
     5521 ctcgtgccgt ccgccccggc cagggccagg cccagcaacg gccgatcgac gagccggccg
     5581 ctccccatcg cgaccccgat gcccacctcg tcgccatgcg cggccagcca cgcctcgagc
     5641 tcgagccgat cggcgtcgtc ggccaccagc cgggggtcga cctgctcgat cgcgcccttc
     5701 gggtctgatc tcggatccgg gaggctggcc gcgctccggc gtcgctcgtg gacgaccggg
     5761 gtctccccgg tctccgtcat gggag
//
LOCUS       DASYGU010000260         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1689041, whole genome shotgun sequence.
ACCESSION   DASYGU010000260 DASYGU010000000
VERSION     DASYGU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689041"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..89
                     /locus_tag="VGO32_07445"
     tRNA            2..89
                     /locus_tag="VGO32_07445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Ser,seq:gga)
     gene            113..381
                     /gene="ffs"
                     /locus_tag="VGO32_07450"
     ncRNA           113..381
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGO32_07450"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            460..2103
                     /gene="dnaX"
                     /locus_tag="VGO32_07455"
     CDS             460..2103
                     /gene="dnaX"
                     /locus_tag="VGO32_07455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV7200623.1"
                     /translation="MTDPTDLVEAITQPASPRRALYRKWRAQTFAEVVGQDAIINTLR
                     RAVATDRIVHAYLLVGPRGTGKTSTARILAKAINCLNRADDGEPCDACTACVAIRDGR
                     AMDVIEIDAASHGLVDDARDLVMRALTAPADLRRRVYIIDEVHMLSPHAFNALLKLIE
                     EPPDHVVFILATTDTHRVPPTIISRTQRYDFRRLPTDVIAAKLTRITTAEGVTAEPEA
                     LALIARLADGGMRDAESLLDQVMAYAGDQVVESSVREAVGLADEEAIAGLLDAYVAGD
                     AVAALQGIADLSDAGRDLGQVAQQLEAEGRRRLLASPGDPALARRLAAVLRAAAEAGT
                     TGARDGRARLHLELLAIDASAGPPARASIPTAIPTAKAEPAAASAERPAATPPAPPPA
                     QPAAVRTPAPAPAAAAKPAPAPAPATPLADGERPTLEVLRAHWGEVVERASPVVKPLI
                     KECRPHELNGAHLTLAFPEEREFLRGLIASRARNIEQLLSEMFGGAWSIECVAASVDL
                     EPLTVAEAIAPGDAPDANALLQRTLEITGGQLVDAPEVR"
     gene            2106..2432
                     /locus_tag="VGO32_07460"
     CDS             2106..2432
                     /locus_tag="VGO32_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV7200624.1"
                     /translation="MNIGQLTKMAQEMQANMARVQAELAEATVEATVGGGAVRVVMTG
                     TQELRSIEIDPATVDPEDVEILQDLVFAAVTDALAQSKRLAADKMGAVTGGMGLPGLG
                     GLPGLR"
     gene            2452..3066
                     /gene="recR"
                     /locus_tag="VGO32_07465"
     CDS             2452..3066
                     /gene="recR"
                     /locus_tag="VGO32_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV7200625.1"
                     /translation="MAGLIAPVARLIEAFNRLPGVGPKTAQRLTYHVLRSPPDEARAL
                     AEALIAVKEQVAYCSTCWNITEVGVDPCLICADPQRDATRICVVEEPLDVLAIERTRE
                     FRGRYHVLHGAISPMDGIGPERIRARELVARAQGGGLEEVILATNPTLEGEATAMYLA
                     DLLGTSVPAITRIARGVPVGGDLEYADDVTLIRSLQGRRQVERG"
BASE COUNT          518 a         1234 c         1132 g          478 t
ORIGIN      
        1 cggagaggtc gcctagaggc ctatggcgcc gcattggaaa tgcggttcgg ggcaacccgt
       61 cgggggttcg aatccccccc tctccgccac cttcgggtac catccggccc ggccgtgctc
      121 aggcggggag ttagcggtgc cctgtgaccc gcaatccgct ctagcggggc tgacatcccg
      181 gctgaggtcg tcgaccttcg cctcccgtga cgtcgtccga cgttgagagt cgggtcccac
      241 gcagcgacgg cccgcgaacc ccgtcagatc cggaaggaag cagcggtaag cggtcctccg
      301 tcgagtgccg tgggagtacc tggctggagc cggactccgt gacagaggcg agtggaccga
      361 ttcccgatgc cgggcgcacg gccgaacctc cctgcccgcg gtgcggtgta gggttagacc
      421 gacatgccga aggggcctcc cgcgaccgat gaacctgcct tgaccgatcc caccgacctc
      481 gtggaggcca tcacccaacc ggccagcccg cggcgcgcgc tgtaccgcaa atggcgggcc
      541 cagacgttcg ccgaggtggt cggccaggac gccatcatca acacccttcg gcgggcggtc
      601 gccaccgacc ggatcgtcca cgcctacctg ctcgtcgggc cacgcgggac gggcaagacc
      661 tccaccgctc ggatcctggc caaggccatc aactgcctga accgcgcgga cgacggtgag
      721 ccgtgcgacg cgtgcaccgc ctgcgtggcc atccgtgacg gtcgggccat ggacgtgatc
      781 gagatcgatg ccgcgtccca cggcctggtg gacgacgccc gggacctcgt catgcgggcg
      841 ctcaccgcgc cggccgacct gcgccgccgg gtgtacatca tcgacgaggt ccacatgctg
      901 agcccgcacg cgttcaacgc gctgctgaag ctcatcgagg agccaccgga ccatgtcgtg
      961 ttcatcctgg ccacgaccga tacccaccgc gtcccgccca ccatcatcag ccggacccag
     1021 cgctacgact tccgtcgcct gccgaccgat gtcatcgccg ccaagctgac ccggatcacg
     1081 accgccgagg gcgtgaccgc cgagcccgag gccctcgccc tcatcgcgcg gctggccgat
     1141 ggcgggatgc gcgacgcgga gtcgctgctc gaccaggtca tggcctacgc cggcgaccag
     1201 gtggtcgaga gctcggtccg cgaggccgtt ggcctggccg atgaggaggc gattgccgga
     1261 ctgctcgacg cctacgtcgc cggtgatgcc gtcgcggccc tgcagggcat cgccgatctc
     1321 tcggatgccg gtcgcgacct cggccaggtg gctcagcagc tcgaggccga gggccgccgc
     1381 cggcttctcg cctcgcctgg ggatcccgcc ctcgcccggc ggctggcggc cgtcctccgc
     1441 gccgcggcgg aagccggaac gaccggggcc cgcgatggtc gggcgcgcct ccatctcgag
     1501 ctgctagcca tcgatgcgag cgcgggcccg ccggcacggg cctctattcc gaccgcgatt
     1561 ccgaccgcga aagccgagcc cgctgccgcc tccgcagaac gtccggcagc cacgccaccc
     1621 gcgccacccc ccgcccaacc ggccgcggtc cggacgccgg ccccggctcc ggccgcggcg
     1681 gccaagcctg cgcctgcccc ggccccggcc accccattgg ccgacgggga gcgcccgacc
     1741 ctcgaggtgc tccgcgctca ctggggcgag gtagtcgagc gagcgagccc ggtggtcaag
     1801 ccgctcatca aggagtgccg cccgcacgag ctcaacggcg cccacctcac ccttgccttc
     1861 ccggaggaga gggagttcct gcgcgggctg atcgcctcgc gggcgcgcaa catcgaacaa
     1921 ctcctgtccg agatgttcgg cggcgcatgg tccatcgagt gcgtggctgc atcggtcgat
     1981 ctcgagccgc tgaccgtggc cgaggccatt gcccccggtg acgctcccga tgccaacgcc
     2041 ctcctccagc ggacgctgga gatcaccggc ggccaactgg tcgacgcccc cgaggttcgc
     2101 tagccgtgaa catcggccag ctgacgaaga tggcgcagga gatgcaggcc aacatggccc
     2161 gcgtccaggc cgagctggcg gaggccacgg tcgaggccac cgtcggcggg ggagcggtcc
     2221 gcgtggtcat gaccgggacg caggagctgc ggagcatcga gatcgatccc gccaccgtcg
     2281 accccgagga cgtcgagatc ctccaggacc ttgtctttgc cgccgtgacc gatgcgcttg
     2341 cccagagcaa gcgcctggcc gcggacaaga tgggggcggt gaccggtggc atggggctgc
     2401 cgggtctcgg cggccttccc ggcctgcgct gacctccgtc ggcatcggtt catggccggc
     2461 ctgatcgcgc ccgttgctcg cctgatcgag gcgttcaacc gcctgcccgg agtcggtccc
     2521 aaaacggccc agcggctcac gtaccacgtc ctccgcagcc cgcccgacga ggcccgcgcc
     2581 ctggccgaag ccctgatcgc ggtcaaggag caggtggcct actgctcgac ctgctggaat
     2641 atcaccgagg tcggcgtgga cccctgcctg atctgcgccg atccgcagcg agacgcgacc
     2701 cggatctgcg tggtcgagga gccactcgac gtcctggcca tcgagcggac ccgggagttc
     2761 cgcggccgct accacgtcct ccatggtgcg atcagcccga tggacggcat cggtccggag
     2821 cggatacggg cacgggagct cgtcgcgcgc gcccagggcg ggggattgga ggaggtcatc
     2881 ctggccacca acccgaccct cgagggcgag gccacggcca tgtacctggc cgacctgctc
     2941 ggcacctcag tgcccgcgat cacccgcatc gcacgtggcg ttccggtcgg cggcgatctc
     3001 gagtacgccg atgacgtgac cctcatccgc tcactccagg gccgccgcca ggtcgagcgc
     3061 ggctgaagtc gcggggctcc gcggcctcgc ggttgacagt ctggggcgtg ctccctagac
     3121 tcaccagtcg cgccggtcga ccaccggcag gtggaaatga acgacggcaa cgtgccgcgc
     3181 cgcatgacca ccggggtttt ctcccaagga ggcctcgaat agaacgaagc cgatccaatc
     3241 ggtggcgttg ccttatcagt tgaagagtga caggaaccca cacgagtcag cgggacaaac
     3301 gtctggacct ttgaaccggt ggagcagccg caaggcgaga ccactggttt tttgttcgga
     3361 ga
//
LOCUS       DASYGU010000261         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_403630, whole genome shotgun sequence.
ACCESSION   DASYGU010000261 DASYGU010000000
VERSION     DASYGU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403630"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VGO32_07470"
     CDS             <1..890
                     /locus_tag="VGO32_07470"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HEV7200626.1"
                     /translation="AAFLAGPETDVRASELLDALEGTYAAFRRRIRSLRDSTSCTCAA
                     CSLVGDLDLKFFVHHGTWVRHRIAGHDELAGTDVIVAHRLLKGAAGSHVSLRGAALLT
                     DAAVAALGVDPQRLGLRPFAETYDELGEVAGQVVDLGERWETARVARRAAGIHERLVD
                     ETIELAIPPSEAWEWLSEPERRGSWDGRAPVEMETVGGRRGIGTTSQCVVGRLSTIEE
                     VIEWRPYERLARRARVGRLGPMTWTWELHPAGIGTKVRLRWTRPQPAGNPRPVPAELV
                     DEQRAALHRLAAAAGGEAC"
     gene            894..1373
                     /locus_tag="VGO32_07475"
     CDS             894..1373
                     /locus_tag="VGO32_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7200627.1"
                     /translation="MRIGQLAREVGVSTDTIRFYERSGWLPRPDRQDNNYREYGVADV
                     EHLRLLIDLRRMEVPLDVAAQMAGWCHSGHCADTTTALPHVIAARRRELADRIAGLRD
                     LDARLADLESHLTAPRTELTMLAAGGPCCDAANSVMGVASEGGCACCAADQGAVQVR"
     gene            complement(1361..2212)
                     /locus_tag="VGO32_07480"
     CDS             complement(1361..2212)
                     /locus_tag="VGO32_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV7200628.1"
                     /translation="MEPFADIQLDTDLTVVGSGHFFRVGEAQFGVRGLIAIESVGPYV
                     PVQHMGPMLMIHDGYFQPGFGIGHHPHRTNERLFYILSGQVEHDDALNHIRGTMEEGD
                     LGRLTEGVRGMFHQEWNGAEQVTRAFILVYHPDTHPPIPVAEFGLLKAADRVQISEGP
                     GVETRELVGPSSGFRVNHSELRRYTDSSLTDGARFDVTLEPNRGLLLYPVDGLVRLSR
                     SGADDLILDAEESVHPEGPGNLAVAWSDSADHRFSLSAVGGPARLLRIEFDRGESDLV
                     LGDPYLT"
     gene            2280..>2566
                     /locus_tag="VGO32_07485"
     CDS             2280..>2566
                     /locus_tag="VGO32_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEV7200629.1"
                     /translation="MATSKLEVLLERGREHDCPDPNCVSMSAAADLPTRFGHFRVVAF
                     GERNGAEHAALIRGEPWDHEKVPVRLHSECLTGDAIGSLRCDCRDQLEASLR"
BASE COUNT          401 a          860 c          920 g          385 t
ORIGIN      
        1 acgctgcgtt cctcgccggt ccggaaacgg acgtccgggc ttcggagctg ctcgacgcgc
       61 tggaagggac gtatgcggcg tttcggcgcc ggattcggag cctgcgggac agcaccagtt
      121 gcacctgcgc ggcctgcagc ctggtcggcg acctggacct gaagttcttc gttcaccacg
      181 ggacatgggt tcggcaccgg atcgcgggcc acgatgagct ggccggaacc gatgtcatcg
      241 tggcccaccg ccttctcaag ggcgcggccg gaagccacgt cagcctgcgc ggtgccgcgc
      301 tgctcaccga tgccgcggtg gcggccctcg gcgtcgatcc acagcgattg ggccttcggc
      361 ccttcgctga gacctacgac gagctcggag aggtcgccgg ccaggtcgtc gacctggggg
      421 agcgctggga aaccgcccgg gtggcgcgac gagcggctgg catccacgag cgcctcgtcg
      481 acgagacgat cgagctggcc atcccgccgt ccgaggcgtg ggagtggctc agcgagccgg
      541 aacgtcgcgg atcgtgggac gggcgggcgc cggtggagat ggagaccgtt ggtggtcggc
      601 gcgggatcgg caccaccagc cagtgcgtcg tgggccgcct atcgaccatc gaggaggtca
      661 tcgagtggcg gccctacgag cggctcgcac gacgggcacg cgtgggacgc cttggaccga
      721 tgacctggac ctgggaactg caccccgcag ggatcgggac gaaagtgcgg cttcgctgga
      781 cacgtcccca accggccggc aacccgcggc cggtgccggc ggagctggtt gacgagcagc
      841 gtgccgcgct ccatcggctg gcggcggccg ccggcgggga ggcctgctga ccgatgcgca
      901 tcggccagct ggcgcgagag gtgggggtct cgaccgacac gattcggttc tacgagcggt
      961 ccggatggct gccgcggccg gatcggcagg acaacaacta tcgcgagtac ggggtggccg
     1021 acgtcgagca cctgcgactg ctcatcgatc tccgccggat ggaggtcccg ctcgacgtgg
     1081 ccgcccagat ggcgggctgg tgccactccg gtcattgtgc ggacaccacc acggcgctgc
     1141 cccatgtcat cgccgcgcgc cggcgtgagc tggccgaccg gatcgccggg ctccgcgacc
     1201 tcgatgctcg gttggctgac ctcgagtccc acctgaccgc cccgcgaacg gaactcacca
     1261 tgctcgcagc cggcgggccg tgctgcgatg ccgccaactc ggtgatggga gtcgcctcgg
     1321 aagggggctg tgcgtgctgc gcggcggacc aaggcgccgt tcaggtcagg tagggatctc
     1381 ccaggacgag atcgctctcg ccgcggtcga actcgatccg cagcaggcga gcggggcctc
     1441 cgaccgcgga gagcgagaag cggtggtcgg cgctgtcgct ccaggctacc gccaggttgc
     1501 caggcccctc gggatgcacc gactcctccg cgtcgagaat gaggtcgtct gcgccggacc
     1561 ggctgaggcg gacgagccca tccaccgggt agaggagcag cccacggttc ggctccaagg
     1621 tgacgtcgaa cctggcgcca tcggtcaggg acgagtcggt gtagcgacgc agctcggagt
     1681 gattgacgcg gaacccggaa cttggcccca ccagctccct ggtctcgacg ccggggcctt
     1741 cgctgatctg gacccggtcg gcggccttca acaggccgaa ctcggcgacg gggatcggcg
     1801 gatgggtgtc ggggtggtag acaaggatga acgcccgggt cacctgctcg gcgccgttcc
     1861 attcctggtg gaacatccca cgtacgccct cggtcaggcg gcccagatct ccctcttcca
     1921 tcgtgccccg gatgtggttc agcgcgtcgt cgtgctcgac ctggccggac aggatgtaga
     1981 acagccgctc gttcgtccgg tgcggatggt ggccgatccc gaatccgggc tggaagtagc
     2041 cgtcgtggat catcagcatc gggcccatgt gctggaccgg cacgtatgga ccgacgctct
     2101 cgatcgcgat caggccgcga acgccgaact gcgcctcgcc gacgcggaag aagtggcccg
     2161 agccgaccac cgtcaggtcg gtatcaagct ggatgtctgc gaacggctcc acggccaaaa
     2221 gcctagccca ggcctgctga aagcgcagaa ttcggacagg cggtatcgtc ccagccaaca
     2281 tggccacatc gaaacttgaa gtgctcctcg agcggggccg cgagcacgac tgtcccgacc
     2341 cgaactgcgt gtcgatgtcg gccgccgcgg acctgcccac ccgattcgga cacttccggg
     2401 tggtggcctt cggcgagcgg aacggcgcgg agcacgcggc cctgatcagg ggtgagccgt
     2461 gggaccacga gaaggtcccg gtccgactcc attccgagtg cctgactggc gacgcgatcg
     2521 gctccctgcg ctgcgactgc cgagaccagc tcgaggcctc cctccg
//
LOCUS       DASYGU010000262         7307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1843331, whole genome shotgun sequence.
ACCESSION   DASYGU010000262 DASYGU010000000
VERSION     DASYGU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843331"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VGO32_07490"
     CDS             <1..972
                     /locus_tag="VGO32_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV7200630.1"
                     /translation="DIAFLAEFTDGQPARSALELAAGAAELATAAGGTAVGLAYGPDA
                     STGAPALGPAGAGRVIVLDSSTNPAVTAAGPLIATIRDLEPQAVLAPATPNGRDLAAV
                     LVGALGLAAFGPIRAVRMEGGQVRCEMSTLQGSVITVSEPTSEAPTTAVILVSAGTYG
                     PREGGSGHADVSEAPPADSPQASVKVVETAAESAAIAALEEADIIVAGGRGVGGEAGF
                     AELERLASALGGVVGASRAAADAGWVPYQLQIGQTGKVVKPALYLAAGISGAIQHRVG
                     MQAADNIVAINRDPDAPIGEFADLFVVGDLFEIVPRLTAEIERRRGA"
     gene            969..1487
                     /locus_tag="VGO32_07495"
     CDS             969..1487
                     /locus_tag="VGO32_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200631.1"
                     /translation="MSTVAVFRRRASDHVDTGQLTSYRTFLAPHLERIEALCDTSGEI
                     AEDLRRRIREPDEIAEPLAAAEAELDEIALAIAERDAPEPLRALHAEFDANLERALRG
                     IVTIERGCGLTRLPHASIYDDGPQAYWKRGHLNIVHAQMRMREIAEVLFAWKTGTPAE
                     ASVSARIQQIGA"
     gene            1566..2036
                     /gene="nrdR"
                     /locus_tag="VGO32_07500"
     CDS             1566..2036
                     /gene="nrdR"
                     /locus_tag="VGO32_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV7200632.1"
                     /translation="MRCPRCNESSTRVIDSRDLENGSTIRRRRECELCAHRFTTYERP
                     EGARLMVVKRDGTRQEFERAKLLAGLMKACEKRPVTLARLDEAADEIETRLRERGDAE
                     VTSKDIGKLATEALRGIDQLAYIRFASVYHSYEDIGTLKREVDRLIRQRSGPGE"
     gene            2033..2623
                     /gene="dcd"
                     /locus_tag="VGO32_07505"
     CDS             2033..2623
                     /gene="dcd"
                     /locus_tag="VGO32_07505"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV7200633.1"
                     /translation="MILSDRDIRAELAAGRIVIDPFDPDAIQPSSVDLHLDRSFRVFR
                     NTRYPFIDVREEQPDLTELVTIGDDEPFILHPGEFVLGSTYERVALPDDLVARLEGKS
                     SLGRLGLLIHSTAGYVDPGWDGNLTLELSNVANLPITLFDGMKIGQISFQRLSSPVER
                     AYGDPSLGSRYRGQTDPTASRYFADFERTRARRSGD"
     gene            2645..3514
                     /locus_tag="VGO32_07510"
     CDS             2645..3514
                     /locus_tag="VGO32_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7200634.1"
                     /translation="MTDPLHDITPAAEAMVFGGGVSVRIVRAGWFRPDAGSFFGVVPR
                     PLWERFAQTDDRGRILCRLNLLIVEAGGKRILVETGTGLRMTARDREIKGVEGGDPVA
                     ALEAVGEDPASFDFVVVSHLHYDHAGGMVDVDGRPAFVNARYVVQRDEAQAAHGDELR
                     LAGIMERDQLDAVQGAGQLAEVHGTVELVDGVTVTRTGGHTRGSQAVLFGRDDGGARG
                     VFFGDLIPTRWQLPIRWTSAYDDYPIEAVEIRREIVSHAAEEGWWCYFTHDPGAQPIQ
                     IEATEKGVTVRAE"
     gene            3607..4071
                     /locus_tag="VGO32_07515"
     CDS             3607..4071
                     /locus_tag="VGO32_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV7200635.1"
                     /translation="MSPDYRSKLRPPLRAAGILLALLVLLPACDALSGVTATPTPRAT
                     ARPEPTPSPTPIPSPPPGAVILEAANRQYSLAVIEGVAGAPTIVYFTNNDDQNHNVTI
                     YRNASKDLELFRGEIFMGPDQMVVYEIPSLAAGEYYFSDYVFPSMHGRFIVR"
     gene            4091..4486
                     /locus_tag="VGO32_07520"
     CDS             4091..4486
                     /locus_tag="VGO32_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV7200636.1"
                     /translation="MSRSARRQGATTLILALLTLAACTGSSPSGSVECRAPVDGVLTI
                     TAANLLFDTACLALPADEAVTIRLVNDDTQPHNLAIYTDSSKETLLFSGDIIDAGEEI
                     EYEVDPIPAGTFYFDCTVHPGMNGSVVVE"
     gene            4503..4579
                     /locus_tag="VGO32_07525"
     tRNA            4503..4579
                     /locus_tag="VGO32_07525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4537..4539,aa:Arg,seq:cct)
     gene            4646..4882
                     /locus_tag="VGO32_07530"
     CDS             4646..4882
                     /locus_tag="VGO32_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200637.1"
                     /translation="MIERLLSVGTGALATYLALVFLNPTDGPDPSGDYLTAVLIGAAV
                     SLIWPLVWLLLIRRRRQKKMDEQVAEEVAKQTGG"
     gene            complement(4889..5677)
                     /locus_tag="VGO32_07535"
     CDS             complement(4889..5677)
                     /locus_tag="VGO32_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200638.1"
                     /translation="MPHLLLDSIHIVGVFVLFVLISMLVFEIGFRLGRWRKNRNPDEQ
                     EGASGTLVGALLGLLAFLLAVAMGIAADRFDARRGLVLTEATTINTAYLRAGYLPEPY
                     RSEVRDLLVEYVPLHIAPEGMREGELVAALARSNEIQDELWVVTEEVANELDRSDIIA
                     LYVESVNDVITVSTQRVTAGIYARVPDTILVFLLLLAFGSIGMVGFGAGQAGKRSIVS
                     AAIMIVALGATFTLVVDLDRPREGFLQVSQQPLIDLERRMSEEP"
     gene            complement(5677..6003)
                     /locus_tag="VGO32_07540"
     CDS             complement(5677..6003)
                     /locus_tag="VGO32_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEV7200639.1"
                     /translation="MQPVVWLLLAIGSEVIATSALKASDGFTHLVPAIVVVVGYAASF
                     YFLALTLRDIPLGVTYAVWSGIGTAAIAIIGVVFFRETLGWAGIAGITLIVLGVVMLN
                     LSGAHG"
     gene            6075..6740
                     /locus_tag="VGO32_07545"
     CDS             6075..6740
                     /locus_tag="VGO32_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV7200640.1"
                     /translation="MRGLAPALVAEAVGTFLFFFIAAGAALAATGDPAAVLLTIALAH
                     GVVLAVLVSSFGAVSGGHFNPAVTLGLWVAGKVDLVRGGSYFVAQMVAAAAAGYALVY
                     VFTDVAPAVPALGAGVDVTEGIILEAIMTMVLLFAVFGTAVDARGPKIGGLAIGFAVA
                     ADILLGGTLTGAAMNPARWFGPALAAGDFSNWYVWWIGPFAGAIIVALLYRFLLSAPE
                     EVG"
     gene            complement(6737..>7307)
                     /locus_tag="VGO32_07550"
     CDS             complement(6737..>7307)
                     /locus_tag="VGO32_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV7200641.1"
                     /translation="GRRPMLVAAALFTLGFSTVFVLLGISVGLIGMALFREPLVRQLA
                     GLVVIGLGLLMTGMFGPVLERLPNPASALPPATARESRALALGAVVAIGWTPCIGPVL
                     GAILAMGASTQDVLSATFLLIAYSLGLALPFLLTAAFLPRVRPALRWLRAHERPIRIA
                     AGLAVAAVGVLIVLDAFTRLAGLFGEFFL"
BASE COUNT         1198 a         2515 c         2486 g         1108 t
ORIGIN      
        1 gacatcgcct ttctcgccga gttcaccgac ggccagcccg cgcgctcggc actcgagctg
       61 gcggccggcg cggccgagct ggccaccgcc gccggtggga cggccgtggg cctggcctac
      121 gggccggacg catccaccgg tgccccagcc cttggaccgg ccggcgccgg acgggtgatc
      181 gtcctcgatt cgtccaccaa cccggcggtg accgccgccg gcccgctgat cgcaacgatc
      241 cgcgacctcg agccgcaggc cgtcctggct cccgcaacgc cgaatggccg ggacctcgcc
      301 gccgtgctcg tcggcgcgct cggactcgct gcgttcggcc ccatccgcgc agtacgaatg
      361 gagggtggtc aggtacggtg cgagatgtcc actcttcagg ggtccgtgat caccgtttcc
      421 gaaccaacca gcgaggcacc caccaccgct gtgatcctcg tcagcgcagg gacctacggt
      481 ccccgtgagg gcggcagcgg ccatgccgac gtgtccgaag ccccgccggc cgattcgccg
      541 caggcgagcg tgaaggtggt ggagaccgcg gccgagtccg cggcgatcgc cgcgctcgag
      601 gaggccgaca tcatcgtggc cggtggtcgc ggagtcggcg gggaggccgg attcgccgag
      661 ctcgagcggc tcgcgagcgc actgggcggc gtggtgggcg cttcgcgggc ggccgctgac
      721 gcgggttggg ttccgtacca gctccagatc ggtcagactg gcaaggtcgt gaagccagcg
      781 ttgtaccttg cggcgggcat cagtggcgcg atccagcacc gggtcgggat gcaggccgcg
      841 gacaacatcg tcgccatcaa ccgcgatccc gacgccccca tcggcgaatt tgccgacctg
      901 ttcgtcgtcg gcgacctgtt cgagatcgtg ccgcgcctga cggccgagat cgaacggcga
      961 cggggagcgt gagcacggtg gccgtctttc gtcgtcgagc gtcggaccat gtcgacaccg
     1021 gtcagctcac gtcctaccgg acgttcctgg cgccccacct ggagcggatc gaagccctgt
     1081 gtgacacctc cggcgagatc gcggaggacc tccggcgccg gatccgcgag ccggacgaga
     1141 tcgcggaacc actggctgcc gccgaagcgg agctggacga gatcgcgctc gccatcgccg
     1201 agcgcgacgc gccggaaccg ctccgcgcgc tccacgcgga gttcgacgcg aacctcgagc
     1261 gggcgctgcg cgggatcgtg accatcgaac gcggctgcgg gctgacccgg ctgcctcatg
     1321 cgagcatcta cgatgacggg ccgcaggcct actggaagcg cggccacctg aacattgttc
     1381 acgcccagat gcggatgcgc gagatcgccg aggtcctctt cgcctggaag accggcacgc
     1441 cggccgaggc atcggtctcg gcccgcatcc agcagatcgg ggcctgacct cccgaatacg
     1501 gcacatcttg tggtatcgtt ggattcggta ccaccagtgg tgtagtccgc accggagagg
     1561 agcctatgcg ctgtccgcgc tgtaacgagt cgagcacgcg cgtcatcgac tcccgggacc
     1621 tcgagaacgg cagcaccatc cggcgccgcc gcgagtgcga gctgtgcgcc catcggttca
     1681 cgacctacga gcgccccgag ggcgcccggt tgatggtcgt caaacgcgac ggcacccgcc
     1741 aggagttcga gcgtgcgaag ctgctggccg gcctgatgaa ggcctgcgag aagcggccgg
     1801 tgacgctcgc gcgcctcgac gaggcggccg acgagatcga gacccggctc cgcgagcgcg
     1861 gcgacgccga ggtgacctcg aaggacatcg gcaagctggc caccgaggca ttgcgcggca
     1921 tcgaccagct ggcctacatc cgcttcgcct cggtctatca cagctacgag gacatcggga
     1981 cgctcaagcg cgaggtcgac cgcctgatcc gccagcggag cgggcccggc gagtgatcct
     2041 gtccgaccgg gacatccggg cggagctggc agccggccgg atcgtcatcg acccgttcga
     2101 tcccgacgcc atccagccgt cgagcgtgga tctccatctc gaccgctcgt ttcgggtatt
     2161 ccgcaacacg cgctacccgt tcatcgacgt ccgtgaggag cagcccgacc tcaccgagct
     2221 ggtgaccatc ggcgacgacg agccgttcat cctccatccc ggtgagttcg tcctgggctc
     2281 gacgtacgag cgagtggccc ttcccgacga tctcgtggcc cgactggagg gcaaatccag
     2341 cctcggtcgt cttggtctcc tcatccactc gaccgccggc tacgtcgatc ccgggtggga
     2401 cgggaacctc accctcgagc tgtcgaacgt ggcgaacctg ccgatcaccc tgtttgacgg
     2461 gatgaagatc ggccagatca gctttcagcg cctgtcgagc ccggtcgagc gcgcctatgg
     2521 cgaccccagc cttggctcgc gctaccgcgg acagaccgat ccgacggcga gccgctattt
     2581 cgctgacttc gagcggacgc gcgcccgccg atcgggcgac tgagtccaac gccatcgggc
     2641 gccgatgacc gatccgctgc acgacatcac ccccgcggcg gaggccatgg tcttcggcgg
     2701 tggggtttcg gtccgcatcg tgcgcgcggg gtggttccgg cccgacgcgg gctccttctt
     2761 cggcgtcgtc ccgcgcccac tctgggaacg gttcgcccag accgatgacc gtggccggat
     2821 cctgtgccgc ctcaacctgc tcatcgtcga ggccggaggg aagcggatcc tggtcgagac
     2881 cggcaccggt ctccggatga ccgcgcgaga ccgcgaaatc aagggcgtgg aggggggcga
     2941 cccggtcgcg gcgctcgagg cggtcgggga ggacccggcg tcattcgact tcgtggtggt
     3001 gagccacctg cactacgacc acgccggcgg catggttgac gtcgatgggc ggccggcgtt
     3061 cgtgaacgcg cgctacgtcg tccagcgcga cgaggcgcag gccgcgcacg gggacgagct
     3121 gcggctggcc gggatcatgg aacgcgacca gctcgacgcc gtccagggcg ccggccagct
     3181 ggccgaggtc cacggcacgg ttgagctggt ggacggggtg accgtgaccc ggaccggcgg
     3241 ccatacgcga ggatcgcagg cggtcctgtt cggccgagac gacggcgggg cccgcggggt
     3301 cttcttcggc gacctgatcc cgacccgctg gcagctcccc atccgatgga ccagcgcata
     3361 cgacgactac ccgatcgagg ctgtggaaat ccggcgcgag atcgtctccc acgcggcaga
     3421 agagggatgg tggtgctact tcacgcacga ccccggcgca cagccgatcc agatcgaggc
     3481 taccgagaag ggcgtcaccg ttcgcgccga gtgagtcggg ggagtgtggt cctgcctgtc
     3541 tgacccatca gtaccgtgcc ggacacgaga gggggcgtgg cattctggca acgggtcaac
     3601 ctatcgatga gtcctgatta ccgatccaag ctccggccgc cgcttcgcgc agcggggatt
     3661 ctccttgccc tgctcgtgct ccttcctgcg tgcgacgccc tgagcggcgt gaccgcgacg
     3721 ccgactcccc gcgccaccgc gcggccggag ccaacgccca gcccgacgcc gatcccaagc
     3781 ccaccgccgg gcgcggtcat cctcgaagcg gcgaaccgcc agtacagcct ggcggtgatc
     3841 gagggcgtgg cgggtgcacc gacgatcgtg tacttcacca ataacgacga ccagaaccac
     3901 aatgtgacga tctatcgcaa cgcgtccaag gacctcgagc tcttccgggg cgagatcttc
     3961 atgggacccg accagatggt ggtctacgag atcccgtcgc tcgccgccgg ggagtactac
     4021 ttctcggact acgtcttccc ctcgatgcac ggtcggttca tcgtccgctg accgggtatc
     4081 atcccggccc gtgagccgat cagcgcgccg ccagggcgcc accactctca tcctcgctct
     4141 gctgaccctt gcggcgtgca cgggcagctc gccgagcgga tcggtggaat gtcgcgcgcc
     4201 cgtggacggg gtgctgacca tcaccgcggc gaatctcctg ttcgacacgg cgtgcctggc
     4261 cctgccggcc gacgaggcgg tcacgatcag gctcgtgaac gacgacacgc aaccgcacaa
     4321 cctcgcgatc tacaccgact cgtcgaagga aactctgctg ttctccggtg acatcatcga
     4381 tgccggggaa gagatcgagt acgaggtgga tcccatcccg gcgggcacgt tctacttcga
     4441 ctgcacggtg cacccgggca tgaacggcag cgtggtggtg gagtaggcgt accctgtccc
     4501 gggcccccgt agctcagctg gatagagcgg caccgtccta aggtgcgcgt cggcggttcg
     4561 agtccgtccg ggggcgccag tttcctcttc cgtccaacga tccctcggca tacgatgccc
     4621 gcacctgcat cacacggaga gcggaatgat cgaacggctg ctgagcgtgg gcaccggcgc
     4681 cctggccacc tacctcgcgc tcgtattcct gaatcccacc gacggcccgg acccgagtgg
     4741 cgactacctc accgcggtct tgatcggtgc ggccgtcagc ctgatctggc cgctcgtctg
     4801 gctcctcctc atccgccgcc gacggcagaa gaagatggac gagcaggttg ccgaggaggt
     4861 cgccaagcag accgggggat gacccggtct acggctcttc gctcatccgg cgctcgaggt
     4921 cgatcagcgg ctgctggctg acctggagga acccttcacg gggccgatca aggtcaacga
     4981 ccaacgtgaa ggtggccccc agcgcgacga tcatgattgc cgcgctgacg atgctccgtt
     5041 ttccggcctg gcccgcgcca aagcccacca tcccgatcga accaaaggcg aggagcagca
     5101 ggaacacgag gatggtgtcc ggcacccggg catagatgcc cgcggtgact cgctgggtgc
     5161 tgaccgtgat cacgtcgttc accgactcga cgtagagcgc gatgatgtcc gagcgatcca
     5221 gctcgttcgc cacttcctcg gtcacgaccc acagctcatc ctggatctca ttggatcgcg
     5281 cgagggccgc caccagttcg ccctcgcgca ttccctcggg ggcgatgtgg aggggcacgt
     5341 actccaccag caggtcgcgg acctcgctgc gatagggctc aggcaggtag cccgctcgca
     5401 ggtaagcggt gttgatcgtc gtcgcctcgg tgagcacaag ccctcgcctc gcgtcgaatc
     5461 gatccgcggc tatccccatc gcgaccgcga ggaggaacgc cagcaggccg agcagggccc
     5521 cgaccagcgt cccgctggcc ccctcctgtt cgtcggggtt gcggttcttg cgccagcggc
     5581 ccaggcggaa cccgatctcg aagacgagca ttgagatcag gacgaacagg acgaagacgc
     5641 cgacgatgtg gatcgaatcc aggaggagat ggggcatcag ccgtgggctc ccgacaggtt
     5701 gagcatcaca acgccaagga cgatgagagt gatgcccgcg attccggccc acccaagtgt
     5761 ctcgcggaag aatacgaccc cgatgatcgc aatcgcggcc gttccgatgc ccgaccacac
     5821 ggcgtaggtg acgccgagcg ggatgtcacg cagggtgagc gccaggaaat agaaactcgc
     5881 cgcgtagccg accacgacca cgatcgccgg cacgaggtgg gtgaaaccgt cggaggcctt
     5941 cagcgcgctg gtggcgatca cctccgagcc aatcgccagc aaaagccaca cgacaggttg
     6001 catcggttcg atagcatggc cgaaaggacg gcactgccgc agcgattcca gtgtcgccag
     6061 cttggaggat cgacatgcga ggcttggcac cggcgctggt cgccgaggcg gtcggaacat
     6121 tcctgttctt cttcatcgcg gccggggccg cgctggcagc taccggcgat ccggcagccg
     6181 tcctgctcac catcgccctg gcccacggcg tcgtgctcgc ggtcctggtc tcgtcattcg
     6241 gtgcggtatc cggcgggcac ttcaacccgg cggtgaccct gggcctgtgg gtggccggca
     6301 aggtcgacct cgtccgtggg ggttcctatt tcgtggccca gatggtcgcc gcggcggcgg
     6361 ccgggtacgc cctggtctac gtcttcaccg atgtcgcccc cgcggtgccg gctctcggtg
     6421 cgggcgtgga cgtcaccgag ggcatcatcc tcgaggcgat catgaccatg gtcctcctgt
     6481 tcgccgtctt cggaacggcc gtagatgcgc gtggaccgaa gatcggcgga ctggcgatcg
     6541 ggttcgcggt cgcggccgac atcctgcttg gcgggacgct caccggtgcg gccatgaatc
     6601 cggcccgctg gttcggcccg gccctcgcgg ccggcgactt ctcgaactgg tacgtgtggt
     6661 ggatcgggcc gttcgcgggc gcgatcatcg tcgcgctgct ctataggttc ttgctctccg
     6721 cgcccgaaga agtgggctag aggaagaact caccgaacag cccggccagc cgggtgaagg
     6781 cgtccagcac gatcaggacc ccgaccgccg cgacagccag cccggccgcg atccggatcg
     6841 gtcgctcgtg ggcgcggagc cagcgcagcg ccggccggac acgcggcagg aaggctgcgg
     6901 tgagcaggaa cgggagcgcc agcccgagcg agtaggcgat gagcaggaag gtggcgctca
     6961 gcacgtcctg ggtggacgcg cccatcgcca ggatcgcgcc aagcaccgga ccgatgcacg
     7021 gggtccagcc gatggcgacc accgctccca gggccagggc ccgcgattcg cgcgcggtgg
     7081 ccggcgggag ggcggaggcg gggttcggca gacgctcgag aaccggcccg aacatgccgg
     7141 tcatgagcag tcccagcccg atgaccacca ggccggccag ctgacgaacg aggggctccc
     7201 ggaagagcgc catcccgatc aggccgaccg agatcccgag caggacgaac acggtgctga
     7261 acccgagcgt gaacagggcg gccgcgacga gcatcgggcg acggccg
//
LOCUS       DASYGU010000263         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1638005, whole genome shotgun sequence.
ACCESSION   DASYGU010000263 DASYGU010000000
VERSION     DASYGU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638005"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..734
                     /locus_tag="VGO32_07555"
     CDS             81..734
                     /locus_tag="VGO32_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200642.1"
                     /translation="MTLIRLLVAAALFGLAVACSPTPAGSSVTATAALDLVRGEDPLF
                     TGVIAHDPEAIGQAGWFEQTETEDGWEIVIRIGWGDCPAGCISEHRWTYTVTVAGEVT
                     LVEESGHPLPTDTAVRGTVVAGPTCPVMTDPPDPSCADRPVSDAELVVLTGDGAEVAR
                     TTSATDGTFAIALASGAYRLEPQPVEGLMGTAEPIEFSVVWGMPIPELVVSYDTGIR"
     gene            complement(739..948)
                     /locus_tag="VGO32_07560"
     CDS             complement(739..948)
                     /locus_tag="VGO32_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200643.1"
                     /translation="MRLAAYLLFPLLILVTGIVYLLIGDEPAGGVMLLFFAAAATFFM
                     WVLVPTFDNEGNTAPIVEDFELPED"
     gene            complement(978..1532)
                     /locus_tag="VGO32_07565"
     CDS             complement(978..1532)
                     /locus_tag="VGO32_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200644.1"
                     /translation="MLSRHSDPRTEDVITFDLVRTGPDGEVVIGSATFEAGLTRIDAP
                     ENVTAALGEQLAQPFVDRVRADERSRGYRRSGQGQVDLLVPGMAEHFVARMRGLWLAF
                     PDQTVVTAREGRVRQAPPQPSTLPDAGEAGPPVVDPAVRRSTLERADNAVSARALVRA
                     ADPDAGTRPAGSVAVRRTDCGWIV"
     gene            1592..1948
                     /locus_tag="VGO32_07570"
     CDS             1592..1948
                     /locus_tag="VGO32_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200645.1"
                     /translation="MELTQEKPYREMVSAQSDDQIDTWAADLFIDFAKRLGVGTAVAS
                     FNRAAGLDARGFQRVFLVGGGPDHVVGIDTAGALAAPIFELPKAVAGLRRTDPDARAK
                     LIDFLVNHREVMSYTP"
     gene            complement(1979..2944)
                     /locus_tag="VGO32_07575"
     CDS             complement(1979..2944)
                     /locus_tag="VGO32_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV7200646.1"
                     /translation="MARTPVFKDNIARLFDEATATELTPGIVSLIESKAPPQIREIPV
                     GRILPNPAQPRLSYEEDSLSELAESIREHGVLQAILVRPAGAQFELIAGERRWRASRL
                     AGLATIPAVVLEFDEETALEVSIIENLQREDVSPLEEAAMYRKMLALGYSVRQLAAKI
                     GKDKGYVENRLRLTDAPPEVRELVAGRKDTISHAYELMKVTDEKTRRKLAKRVAAGEL
                     TLAKLRRLTGSRDPKVKEPTERPKRRSKTAAGVAVAARNTDDALMDLKGRLATVVDEL
                     GDLLRQHEVIDQIPSTNRDNLAKYLVITKLKLENAIAIVRATRDE"
     gene            complement(2937..3704)
                     /locus_tag="VGO32_07580"
     CDS             complement(2937..3704)
                     /locus_tag="VGO32_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV7200647.1"
                     /translation="MTKVISLANQKGGVGKTTCTINLGGALAELGYRVLCIDMDPQAN
                     LTVGLGISLADVHRSMADVLNEDRLALDEVIVETKVAGMSVAPATLELASTEVELFTV
                     IGREMVLRDAISGSADRRFDVILIDCPPTLGLLTINSLVASSRVIIPVQTQFYAIKGL
                     TALIKVINTIRVKLNHDLEILGLLPTFYDGRTILAREMLENLRELGDHRVFNTMIKNT
                     VKLGEAPLTGRPVTEYAPSSAAAHAFRDLAREVIELG"
     gene            3882..3954
                     /locus_tag="VGO32_07585"
     tRNA            3882..3954
                     /locus_tag="VGO32_07585"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3915..3917,aa:Ile,seq:cat)
     gene            4013..>4266
                     /locus_tag="VGO32_07590"
     CDS             4013..>4266
                     /locus_tag="VGO32_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7200648.1"
                     /translation="MRDAARLGGGEERIAQQHARGKLTARERLEILLDDDSFAELDAF
                     VTHRTTDFGLEEEQYLGDGVVTGWGTIDGRTVFVYAQDFTV"
BASE COUNT          665 a         1406 c         1485 g          710 t
ORIGIN      
        1 gaacctgtgg ttcgccgtgc gccagatgat tccctggtga cggtccggaa cctcaccacg
       61 cctgcgggcg tccagcgaac atgacgctca tccggttgct ggtcgccgcc gcgctgttcg
      121 ggcttgccgt cgcctgctcg cccaccccgg ccgggtcttc ggtcaccgct accgcggcgc
      181 ttgatctcgt gcgcggcgag gacccgctct tcacgggggt catagcgcac gaccccgagg
      241 ccatcggcca ggccggctgg ttcgaacaga ccgagaccga ggacggctgg gagatcgtca
      301 tccgcatcgg ctggggggac tgcccggcgg gatgcatcag cgagcaccgg tggacctaca
      361 ccgtcaccgt ggccggtgag gtcaccctcg tcgaggagtc cggtcacccg ctccccaccg
      421 acaccgcggt ccggggcacg gtcgtggctg gaccgacctg cccggtcatg accgatccgc
      481 ccgacccgag ctgcgccgac cgacccgtga gcgatgccga gctcgtcgtg ctgacggggg
      541 acggggcgga ggttgcgcgg acgacgagcg cgactgacgg aacgttcgcc atcgctctcg
      601 cctctggggc ctaccgcctc gagccgcagc ccgtggaagg gctgatgggc accgccgagc
      661 ccatcgagtt ctccgtcgta tggggcatgc cgatcccgga gctggtcgtg tcctacgaca
      721 ccgggatccg ctgacggctc agtcctcggg aagctcgaaa tcctcgacga tcggggcggt
      781 gttgccctcg ttgtcgaacg tcggaaccag gacccacatg aagaaggtgg cggcggccgc
      841 gaagaacagg agcatcaccc ctccggccgg ctcatcgccg atcagcaggt agacgatccc
      901 ggtgacaagg atcaggagcg ggaacagcag gtacgcggcg agcctcatgg cgtcggcatc
      961 atagcggtgg cgacgcccta gacgatccag ccgcagtcgg tgcgccgaac ggcgaccgat
     1021 cccgccggcc gggtgccggc atcggggtcg gcggcgcgca ccagggcacg ggcgctgacc
     1081 gcgttgtcgg cccgctcgag cgtcgagcgg cggacggcgg ggtcgaccac gggcggaccc
     1141 gcctccccgg cgtcgggcag ggtcgacggc tgcggagggg cctgacggac gcgcccttcg
     1201 cgggcggtca cgaccgtctg atccgggaat gcgagccaca gtccccgcat ccgggccacg
     1261 aagtgctcgg ccatgccggg gacgaggagg tccacctgtc cctggccgga gcgccggtag
     1321 ccgcgtgacc gctcgtcggc ccggacccgg tcgacgaagg gctgcgcgag ctgctcgccg
     1381 agggcggcgg tcacgttttc cggcgcgtcg atgcgggtga gcccggcttc gaaggtcgcc
     1441 gacccgatga ccacctcccc gtccggaccg gtgcggacga ggtcgaaggt gatcacgtcc
     1501 tcggtgcgcg gatcgctgtg gcgggacagc atgggccgag gatagcagcg gcgtcgggct
     1561 agaatccgcc cgcactgacc gaggaggttc cgtggaactg acccaggaga agccctatcg
     1621 cgagatggtc tcggcccagt ccgacgacca gatcgacacc tgggccgccg acctgttcat
     1681 cgacttcgcc aagcgtctcg gggtcggcac cgcggtggcc agcttcaacc gggcagcggg
     1741 cctcgacgcg cggggcttcc agcgcgtgtt cctcgttggt ggcgggccgg accacgtggt
     1801 tgggatcgac accgcgggcg ccctcgccgc cccgatcttc gagctgccga aggcggtggc
     1861 cggcctgcgc cgcaccgatc cggacgcgcg ggccaagctg atcgacttcc tcgtcaacca
     1921 ccgcgaggtc atgtcctaca ccccctagcc ccgcccgggt gggcagcccg gagcgtggct
     1981 actcgtcgcg cgtggcgcgg acgatggcga tggcgttctc cagcttgagc ttggtgatga
     2041 ccaggtactt ggccaggttg tcccggttgg tggacgggat ctggtcgatg acctcgtgct
     2101 ggcggagcag gtcgcccagc tcgtcgacaa cagtcgccag tcggcccttg aggtccatta
     2161 gcgcatcgtc ggtgttccgc gcggcgacgg ccaccccggc cgcggtcttc gaacgtcgct
     2221 tcggtcgttc ggtcggctcc ttgaccttgg ggtcgcggct gccggtcagc cggcggagct
     2281 tggccagggt cagctccccg gcggcgacgc gcttggcgag cttccggcgc gtcttctcgt
     2341 cggtcacctt catcagctcg taggcgtggc tgatcgtgtc cttccggccc gccaccagct
     2401 cgcggacctc cggcggggca tcggtcaggc gcagccggtt ctcgacgtag cccttgtcct
     2461 tgccgatctt cgccgccagc tggcgaaccg aatagccgag ggcgagcatc ttgcggtaca
     2521 tcgccgcttc ctcgagtggc gacacgtcct cgcgctggag gttctcgatg atcgacacct
     2581 cgagcgcggt ctcctcgtcg aactcgagga cgacagccgg gatggtggcg agaccggcga
     2641 gccgtgacgc gcgccatcgg cgctcgccgg cgatgagctc gaactgagcg ccggcggggc
     2701 ggaccaggat ggcctgcagg acgccatgct cccggatcga ctcggccagc tcgctcaggg
     2761 agtcctcttc gtacgagagc cgcggctggg ccgggttggg aaggatccgc ccgaccggga
     2821 tctcccgaat ctgaggcggt gccttggact cgatgagcga gacgatgccg ggcgtcagct
     2881 ccgtggcagt cgcctcgtcg aagagacggg cgatgttgtc tttgaagacg ggtgttctag
     2941 ccaagttcga ttacctcccg ggccaggtca cggaatgcat gggcggcagc cgagctgggc
     3001 gcgtattcgg tcaccggtcg gccggtgagc ggggcctcac cgagtttcac ggtgttcttg
     3061 atcatggtgt tgaagacccg atggtctccg agctcgcgga ggttctcgag catctcgcga
     3121 gcgaggatgg tgcggccgtc atagaaggtc ggcaggaggc cgaggatctc gaggtcatgg
     3181 ttcagcttga cgcggatggt gttgataact ttgatcagcg cggtcagccc cttgatcgca
     3241 tagaactggg tctggaccgg gatgatcacc cgggacgagg ccaccaacga gttgatggtc
     3301 aacaggccga gggtgggcgg gcaatcgatg aggatcacgt cgaagcgtcg gtcggccgaa
     3361 ccggagatcg cgtcacggag caccatctcg cgaccgatga ccgtgaacag ctcgacttcg
     3421 gtcgatgcca gctcgagggt ggccggcgcc acggacattc ccgccacctt cgtttcgacg
     3481 atcacctcgt ccagcgccag gcgatcctcg ttcagcacgt cggccatcga ccgatgaaca
     3541 tccgcgagcg aaatcccgag gcccacggtg agattcgcct gcggatccat gtcgatgcac
     3601 agcacccgat agccgagctc ggccagggcg ccccccaggt tgatggtgca ggtcgtcttc
     3661 ccgacgccgc ccttctgatt cgccagcgag atgactttgg tcaaggcaaa caccttccgc
     3721 ggatgcatgt cgggctgctg aaatcgcgaa ttgcgatccg tcatcgatga tggcagggtc
     3781 gacgcccggg gtcacccggg ttatccacaa cgtccccgac tgtcgatgtc tttgtgcaaa
     3841 gacgaggcca tatactcgga cttgtccacc gccggctccc cgggcccata gctcagtggt
     3901 cagagcatcc ggctcataac cggctggtcg caggttcgat ccctgctggg cccacatccc
     3961 aatttgtgga ggatcggtgt cgaccaacga caagctccag cacctcgccg acatgcgcga
     4021 cgcagccaga ctgggtggtg gcgaggaacg cattgcgcag cagcatgccc gcggcaagct
     4081 gacggcccgc gagcggcttg agatcctgct cgatgacgac tcgttcgccg agcttgatgc
     4141 gttcgttacc catcgcacga ctgattttgg gctcgaggag gagcagtacc tgggggacgg
     4201 cgtcgtcacc gggtggggga cgatcgatgg ccggacggtg ttcgtctacg cgcaggactt
     4261 cacggt
//
LOCUS       DASYGU010000264         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_249243, whole genome shotgun sequence.
ACCESSION   DASYGU010000264 DASYGU010000000
VERSION     DASYGU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249243"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="holA"
                     /locus_tag="VGO32_07595"
     CDS             <1..1110
                     /gene="holA"
                     /locus_tag="VGO32_07595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV7200649.1"
                     /translation="WDRFPPAPGPRRSGRVGSDHGERTCPMSGSVLLAHGDDAYTIDR
                     AVAAFGERAGVADLVVLTPERTPDEALLQRAAVEVASVGLFGAHAVVLRQPLRAAGTS
                     GSAADRLIALVEGVPDGAVLALAELRPSRDVGRPPALLSRLEAAVRAADGEVLVCPAP
                     RRDELRGWVTAHASELGIEIRPPAAAALAQRVGGVWENDIERSEQTRVADGELRKLAI
                     AVEDRPIEVADVEALVTDVRPPSVNAIANAVERRDGRKAAEALQRALDEGEPVLKIMA
                     ALENRLVDLIVARDLISRGATAAEMTKRLRPGNPAAAERTATAARRYSGDELDAMLAG
                     LFEADLALKSNAMEPEAALAAWLGEFVLGAARVGG"
     gene            complement(1121..1384)
                     /locus_tag="VGO32_07600"
     CDS             complement(1121..1384)
                     /locus_tag="VGO32_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200650.1"
                     /translation="MTTTRKRDITAVETCRYCGGGLLTSVFDTSFRDESIEERLFFAL
                     PGALCLPCKQLYIDHQLIGILGLSNARCTFAIESDRVLRAGAA"
     gene            complement(1539..1895)
                     /gene="rsfS"
                     /locus_tag="VGO32_07605"
     CDS             complement(1539..1895)
                     /gene="rsfS"
                     /locus_tag="VGO32_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV7200651.1"
                     /translation="MHAELDPAQLAHRIVEIVSDHKGSDVVMLRTGDLTSLADYFVIA
                     TGRSDRQVSALAGALVEELRAQGVRPLGVEGRSGTRWVLLDFNDVIVHLFTPDERERY
                     DLEKLWSAAPAVVRLV"
     gene            complement(1948..2592)
                     /gene="nadD"
                     /locus_tag="VGO32_07610"
     CDS             complement(1948..2592)
                     /gene="nadD"
                     /locus_tag="VGO32_07610"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV7200652.1"
                     /translation="MESTRPRPVRRAAAPERIGILGGTFDPPHVGHLWLATLAADALG
                     LAKVFLVPAAAPPHKRRRAISHAADRILMTRLAVANDPRLDVSLVEMEREGPSYTVDT
                     LVELKARHADADLVLIMAADSLAQIPSWREPERLLELAIWAVGPRPGTPLPSRADLAA
                     RYGRSAARIHLLSGPALDISASHVRRRVAAGRAIRYLVPRAVEELILERGLYRR"
     gene            complement(2649..>2997)
                     /gene="cgtA"
                     /locus_tag="VGO32_07615"
     CDS             complement(2649..>2997)
                     /gene="cgtA"
                     /locus_tag="VGO32_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEV7200653.1"
                     /translation="DGSGIDRLLVALAAALEEADRHEEASPPAPARRLHRFDPTADGW
                     EVLVEADGLRVRGRSIERSAARTDFTNEESRDRFQRRLERMGVDAELRRQGARDGTLV
                     RIGAQELEWGDEE"
BASE COUNT          429 a         1030 c         1121 g          417 t
ORIGIN      
        1 tgggaccgct tcccgccggc gcccggtccg cggcggtcgg gccgggtagg atcggaccat
       61 ggcgagcgga cgtgcccgat gagcggatcg gtgctcctgg cccatggcga cgacgcgtac
      121 acgatcgacc gcgcagtcgc cgcgttcggc gagcgggccg gcgttgccga tctcgtcgtc
      181 ctgaccccgg agcggacccc ggacgaggcc ctcctgcagc gggcggcggt cgaggtggcc
      241 agcgtcggcc tgttcggggc tcacgccgtg gtcctccggc agccactgcg ggcggccggc
      301 acatcgggat ctgccgctga ccgcttgatc gcgctggtgg agggcgtccc ggacggagcg
      361 gtgctggccc tcgccgagct gcgaccgtcg cgcgacgtcg gccggccgcc ggcccttctg
      421 tcgcggctcg aggccgcggt gcgggccgcc gacggcgagg tgctggtctg tccggcgccg
      481 cgccgggacg agctgcgcgg atgggtcacc gctcacgcgt cggagctcgg gatcgagatc
      541 cgcccaccgg cggcggccgc cctggcccag cgggtcggcg gggtgtggga gaacgacatc
      601 gagcgcagcg agcagacacg ggttgccgat ggcgagctcc gcaagctcgc gatcgcggtc
      661 gaggatcgac cgatcgaggt cgccgatgtc gaggcgctcg tcacggacgt gcggccgcca
      721 tcggtcaatg ccatcgccaa cgcggtcgag cgacgggatg gccggaaggc ggcggaagcg
      781 ctgcagcggg ccctggacga aggcgagccg gtcctgaaga tcatggccgc cctggagaac
      841 cggctggtcg atctcatcgt ggcgcgcgac ctgatcagcc gaggggcgac cgcggccgag
      901 atgacgaagc gactgcggcc cggaaacccg gcggcggccg aacggacggc aaccgcggcg
      961 cggcggtaca gcggggacga gctggatgcg atgctggccg gcctgttcga ggccgatctc
     1021 gctctcaagt cgaatgcgat ggaaccggag gccgcgctgg cggcgtggtt gggcgagttc
     1081 gtgctgggcg ccgcccgggt gggcggctag ccggatcggt tcaggcggct ccggcgcgga
     1141 ggacgcggtc gctctcgatc gcgaatgtgc atcgcgcgtt cgagaggccg aggatcccga
     1201 tcagctggtg gtcgatgtac agctgcttgc atggaaggca cagggcaccc gggagcgcga
     1261 agaagaggcg ctcctcgatg ctctcgtcgc ggaaggacgt gtcgaacacc gaggtgagca
     1321 gcccgccacc gcagtagcgg caggtctcga ccgcggtgat gtcgcgcttc cgagtcgtgg
     1381 tcatgggggc gagaatggcc gccgccgggc cagccctcaa taggaccccc gtacgccagg
     1441 ctggtacccg ccttatgacc gaacgcgggg tcggggtggt gcgtgcacgc tccacgcgat
     1501 gcgtcggact ggtactcccg gggtacctcc ggcgcggctc agaccaggcg gacgacagcc
     1561 ggggcggcgc tccacagctt ctcgaggtcg tatcgctcgc gctcgtcggg ggtgaacagg
     1621 tggacgataa cgtcgttgaa gtcgagcagg acccagcgcg tccccgatcg accttcgacc
     1681 ccgagcggcc gcacgccctg cgcgcgcagc tcctccacca gcgcgccggc gagcgccgac
     1741 acctgccggt cggagcggcc ggtcgcgatc acgaaatagt cggccaggga ggtcaggtca
     1801 ccggtccgca gcatgacgac gtcgcttccc ttgtgatctg acacgatctc gacgatccgg
     1861 tgcgcgagct gcgcagggtc cagctcagca tgcatgggtg tcacgttcgc gttcggttcc
     1921 tgcaggatcg tcctccggtg cctcgtgtca gcgtcggtac aggccgcgtt cgaggatcag
     1981 ctcctcgacc gcccgcggga caaggtaccg gatggcgcga ccggccgcca cgcggcgtcg
     2041 gacgtggctc gcgctgatgt cgagcgctgg tccactcagg aggtggatac gagccgccga
     2101 tcgcccgtac cgagcggcga gatccgctcg cgacggcagc ggcgtgccgg gtcgcggacc
     2161 caccgcccag atggcgagct cgagcaggcg ctccggttca cgccacgacg gaatctgggc
     2221 caggctgtcc gcggccatga tcaggacgag atcggcgtcg gcgtgacggg ccttcagctc
     2281 gaccagggtg tcgaccgtgt acgagggacc ctcccgctcc atttcgacca gtgacacgtc
     2341 caggcgcggg tcgttggcca ccgccagtcg ggtcatcaag atccgatccg ccgcatggct
     2401 gatcgcgcgg cggcgcttgt gcggcggcgc ggcggccggc accaggaaca ccttggccaa
     2461 cccgagcgca tcggctgcca gggtggccag ccacaggtgc cccacgtggg gcggatcgaa
     2521 cgtgccgccc aggatcccga tccgttcggg ggcggcggcc cggcggacgg gacgcggccg
     2581 ggtggactcc accggccgca cgaccgggac aagccgcgag gcaccgcggg tcgacggtgg
     2641 gctggccttc attcttcgtc gccccactca agctcctggg cgccaatccg taccagcgtt
     2701 ccgtcgcggg cgccctgccg gcggagctcg gcgtcgacgc ccatccgttc caggcggcgc
     2761 tggaagcggt ccctcgactc ctcgttcgtg aagtcagtgc gagcggccga ccgctcgatc
     2821 gaccgccctc gcacccgcag accatcggcc tcgacgagga cctcccagcc gtccgcggtt
     2881 gggtcgaacc gatggagacg acgggccggg gcgggcgggc tggcttcctc gtgccgatcg
     2941 gcctcctcca acgccgcggc aagcgcgacc aggaggcgat caatcccgct cccgtca
//
LOCUS       DASYGU010000265         9349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1535157, whole genome shotgun sequence.
ACCESSION   DASYGU010000265 DASYGU010000000
VERSION     DASYGU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535157"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VGO32_07620"
     CDS             <1..1088
                     /locus_tag="VGO32_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HEV7200654.1"
                     /translation="IRYAENPPKRYQDVYPFDFRGPDRDARVALWEAWRELVLTWAAR
                     GVRIFRVDNPHTKPVVFWAWLIREVQARYPDVIFLAETFTRHKPMRLYSKAGFTQSYT
                     YFTWRTDAKSLRKYFTELTLTEMREYYRGNIFPNTPDINPHYLARGRPTFVSRLVLAA
                     TLSGLYGIYSGFELLEHRRIGRTEEYADSEKYEIVTRDWDAPGNLNGLIAQLNGLRAA
                     WPALRRTETLRFEPVRGTRTLFYRKALPVGRIDLLSEEPTRWRQPVWVAVNVQPTRSE
                     QAVLRPTWSEVGIDLSRPYRYTDLLTGKTRERRGRTITVTLGPDRQFVIFTISQDPGP
                     KAAARPARRPRRPERDRGPENRGERRP"
     gene            1092..1862
                     /locus_tag="VGO32_07625"
     CDS             1092..1862
                     /locus_tag="VGO32_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7200655.1"
                     /translation="MPLPGLTFLGHSTVLIELEGLRILTDPILVDRLMFLARVVRPVD
                     PALFADIDLVLLSHLHIDHFDRRSLARIKSEPEIVTCPGGAGLLRSWGWSRVKELAPG
                     ETHQVGPVRITATPADHPGFRPPFGPPGTAVGFVIESGQARLYFAGDTDLLPEMETMI
                     EGELDVAFLPISGWGPRLGPGHMNPARAAEAVVRLRPRFAVPIHWGTLWPYGFGRFTA
                     DRLEGPPAEFVASLDERADSTNARALIARPGERVPFVP"
     gene            1896..3983
                     /locus_tag="VGO32_07630"
     CDS             1896..3983
                     /locus_tag="VGO32_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV7200656.1"
                     /translation="MSSWFRRTAAAYRAQAHALRRLRPTPLGVLVRVLLSVVSAWLSL
                     LITIALAPGIDREPGTSVLLAVAAIGAADFLIRPLLLAAVLPIGFIAVFAVGFLYRAA
                     SFFFILPLIGFDLGGFGSALVGAGIYALVSTIFGGMVGMTDEESYGQRVVNQVIKDQE
                     NVPHSDVPGVVVIQVDGLAFPLLAAQVRAGTLSNLSRWIRSGSHRMMEYTADLPSQTS
                     TGQAGLLHGNAAGIPAFRWYEKERRRMMVSNRPADAAEIEARISDGNGLLANGGASID
                     NMFSGDATHVRMVMSKLHSNLDKAGRDRSMYYFLLNPYSIGRTIVRTIGEIGKELYQG
                     WQQRRRGIEPHMHRGLKFAGLRAMTNVFLRDLNHALVAEQMLRGTPVMYCDFVDYDDI
                     AHHVGPERQEALQSLSGIDRSIGRLERMVPDCPRPYRFVIVSDHGQSQGATFRQRYGE
                     TIEEWLHKQIGADVTRTATAPVEEVGRMSTVLTEATSADGATAALGRRALRGSMDDGA
                     VELGVLGDEAAKEPSGELPDLVVCASGNLGLVYFGVAEHRMSLPEIEAAFPGMMDKLV
                     AHDGIGFALVRSGDGHPLAIGPDGRHDLSDGSVTGTDPVAQFGPDAPDQLRHLDGIDH
                     VGDLVLISRLDTGTDEVAAFEELIGSHGGMGGWQTQPCLVYPADWPAPDGRLMGAPAV
                     HRQLKRWIAAEQG"
     gene            3993..5048
                     /locus_tag="VGO32_07635"
     CDS             3993..5048
                     /locus_tag="VGO32_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV7200657.1"
                     /translation="MGIFDGVLGIFSHDLGIDLGTANTLVTVRHRGIVISEPSVVAME
                     TRTKRVLAIGAEAKRMVGRTPANIVAIRPLRDGVISDFDVTEQMIRYFIEKVHDRYFR
                     IPRPRVLVGIPSGVTEVEKRAVRDATLNAHARWARLIEEPMAAAIGAGLPVSEPSGSL
                     IVDIGGGTTEVAVISLGGIVVSRSIRIGGDEMDQDIVNFARREYNLLMGERTAEEIKI
                     AVGSAYADLNPRTVTFRGRDLLTGLPRSVEVSSEQIREALEPSVAAIIQAIRDTIEET
                     PPELVADIMDQGIVLAGGGALLAGLDRRTAEATQMPVHVADDPLTCVVRGTGRVLEEL
                     DTLERVLITEQYTRAPR"
     gene            5056..5952
                     /gene="mreC"
                     /locus_tag="VGO32_07640"
     CDS             5056..5952
                     /gene="mreC"
                     /locus_tag="VGO32_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV7200658.1"
                     /translation="MRGASFPSGTPGAGPHRRTGNGLLWAVLVAVSLGLVIVSGTEPA
                     RSVQELTAGALAPVHAAIAGIGDGIADVVETVGAIDRLRAEAEELRSTLAGAEQRIAE
                     LQEAARENAQLRSLLGLQAVLGWDLVPARVVAAGTGAVSWEVAIDVGRDDGVRTGMPV
                     VGAASGGGALAGIVVQSGLDRSVVQLTVDPRSRVVARDQQTDALGVVQGQPGGQLVMT
                     QVAVTDEVAVGDTIITAGLELAEVAASQYPRGLLIGTVSALETDANGLTRTVFVRPAL
                     DPRAVEWLMVVRSAAPEASPQP"
     gene            6141..8153
                     /locus_tag="VGO32_07645"
     CDS             6141..8153
                     /locus_tag="VGO32_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7200659.1"
                     /translation="MHERALPDPWRLALVLGLAFALAVSAVFHPIPVRGAAVGQKVVV
                     IVGPVGGGSIQTNYLNKGEAIADAAEARGATVVRVFSPNATYAAARAAVSGANVIVYI
                     GHGSGFPNPYAGTNQPNFNNGWGLNKDTSHGHQHVIGTSLVYCGEAVLEGKASYAGCT
                     GGKINPAPNFVMVYSNACYAPGAGETEQVAVSSEATALARVSNYSRPILALGGTYFAT
                     DLGSKSLVETILDNPNAGWGQIYTMASAYVSGAVKTFVHPKFSTMQAWLQKSSGPGPS
                     VSYFNAFAGDPSRTPAGGTGAPVPDLAKPKVTASSPFTFQTGVSPSADVTATFDKSMV
                     GLDDWNGMELYEASDPGDAMDASVSWDEGSRTVTLHPDSSLQPDTWYVVNFGDDVEDI
                     WGNPLGDASWGFRTGGGSSPVPAATVYGPSTPLWFNPGTYVGRKVDAYGHVTSSKSGT
                     LSATSSAPTAQKGVLPGQSGTWYLITAGLWSGMWIQESAGTVLGTPPPPPPAPIATYS
                     PPQSLWFAPGTYVGRQFNAYGAVVASKSYTLGVASAAPTSIKSTVLTQSGVWYYITAG
                     VWGGYWVQETAATTLGTPPPPPPGTEVYSPAKTLVFAAGTYVGRKFDASGNVTATKSY
                     TLAVSSAAPTSQKGPIPNQTGNWYLITAGVWAGYWIQESPGTTLAP"
     gene            8309..>9349
                     /locus_tag="VGO32_07650"
     CDS             8309..>9349
                     /locus_tag="VGO32_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEV7200660.1"
                     /translation="MTRPASIVRSLVATALAVATVAGAPAPLPVAASSPPSSAWTVPA
                     ELPNAPIRLDDGTVLPMPPAGYLSRPSVPATLDAEHADDPLAFTPGGRPTVDLAILGA
                     SIATDGAPSVLSLGLANPTTSAAADLTALPNGLKREVFGFLPYWMLDAGTLQWMQYQL
                     VSTIAYFGVAAKPDGTLATYATGWTAWNSSAMTGVINAAHARGVKVVLSVTMMAYDGG
                     AQQAALLGSAANRVKLINAIVATVRARNADGVNLDFEPVAVAQRDQYTSFVRQLKAAL
                     VAAGAGSRLTVCTMAGAGTWATGYDLAGLVAPGAADSLFVMGYDFSWSGSARAGGVAP
                     IESPYILDVNQSV"
BASE COUNT         1387 a         3331 c         3157 g         1474 t
ORIGIN      
        1 cgatccggta cgcggagaac ccgccgaagc gctaccagga cgtctatccg ttcgactttc
       61 ggggccccga ccgtgatgcc cgggtggcgc tgtgggaggc gtggcgcgag ctggtcctga
      121 cctgggccgc gcgcggcgtg cgcatcttcc gggtcgacaa cccgcacacc aagccggtcg
      181 tcttctgggc atggctcatc cgcgaagtcc aggcccgcta ccccgacgtc atcttcctgg
      241 ccgagacctt cacccgccac aagccgatgc ggctgtactc caaggcgggg ttcacccagt
      301 cgtacaccta cttcacctgg cggaccgatg cgaagtcgct ccggaaatac ttcaccgagc
      361 tgacgctgac cgagatgcgt gagtactacc gcggcaacat ctttcccaac acgccggaca
      421 tcaaccccca ttacctggct cggggtcgcc cgacgttcgt gagccgcctc gtcctggccg
      481 ccaccctgtc gggcctgtac ggcatctact ccggtttcga gctgctcgag catcgccgga
      541 tcggccggac ggaggagtac gccgacagcg agaagtacga gatcgtgacg cgggactggg
      601 atgcgcccgg gaacctgaac gggctcatcg cccagctgaa cggcctgcga gccgcgtggc
      661 ccgcgctccg ccggacggag acgctgcgat tcgagcccgt tcgcggcacg cgaaccctgt
      721 tctaccggaa ggcgctgccg gtcggtcgca tcgacctgct ctcggaggag ccgacccgct
      781 ggcggcaacc ggtctgggtg gcggtcaacg tccaacccac gcgttcggaa caggcggtct
      841 tgcggcccac ctggtcggag gtcggtatcg atctctcccg cccgtatcgg tacaccgacc
      901 tgctgaccgg gaaaacccgc gaacgacggg gacgaacgat caccgtcacc ctcggtcctg
      961 atcggcagtt cgtcatcttc acgatctctc aggatcccgg gccgaaggcg gctgcgcgtc
     1021 cggcacgccg cccacgccgg ccggagcgcg accgaggccc cgagaatcgc ggcgaacgga
     1081 ggccctgacc gatgccgctc cccgggctga cgttcctcgg tcactccacg gtcctgatcg
     1141 agctggaggg gcttcggatc ctgaccgatc cgatcctcgt cgatcggctc atgttcctgg
     1201 ctcgcgtcgt ccgcccggtc gatccggccc tgtttgccga cattgacctc gtcctgctga
     1261 gccacctcca catcgaccat ttcgatcgcc ggtcgctggc caggatcaaa tccgagccgg
     1321 agatcgtgac ctgcccgggc ggcgccggtc tcctccggag ctggggctgg tcccgggtga
     1381 aggagctggc tcccggcgaa acgcaccagg tcggcccggt tcggatcacg gcgactcccg
     1441 ccgatcaccc cggcttccgc ccgcccttcg ggccccccgg aacggcggtg gggttcgtca
     1501 tcgagtcggg ccaggctcga ctgtacttcg ccggtgatac cgatctcctc ccggagatgg
     1561 aaaccatgat cgagggcgag ctcgacgtcg ccttcctgcc gatcagtggc tggggtccgc
     1621 ggctggggcc cggccacatg aacccggctc gcgccgcaga ggccgtcgtg agactccggc
     1681 cacggttcgc ggtgcccatc cactggggca cactgtggcc gtacggtttc ggccggttca
     1741 cggccgaccg gctggaaggt cccccggccg agttcgtcgc cagcctcgac gaacgggcgg
     1801 actccaccaa tgccagggcg ttgatcgccc gccccggcga gcgggtaccc ttcgttccct
     1861 gaccccccgc ccccgtcttg aggtttccga cgcccgtgag ctcctggttc cgccgcaccg
     1921 ccgccgccta ccgcgcccag gcccacgccc tccgtcgcct gcgcccaacc ccgctcggcg
     1981 tgctcgttcg cgtcctcctg tcggtggtct ccgcctggct gagcctgctg atcacgatcg
     2041 ccctcgcgcc gggcatcgat cgcgagcccg ggacctcggt cctgctcgcg gtggcggcca
     2101 tcggggcagc ggacttcctg attcggcccc tcctcctggc ggccgtcctg cccatcggct
     2161 tcatcgccgt cttcgccgtc ggcttcctgt atcgcgcggc gtcgttcttc ttcatcctgc
     2221 cgctcatcgg cttcgatctc ggcggctttg gcagcgccct ggtgggggcc gggatctacg
     2281 ccctggtcag cacgatcttc ggcggcatgg tcggaatgac ggacgaggag tcgtacggcc
     2341 agcgggtcgt caaccaggtg atcaaggacc aggagaacgt cccccacagc gacgtgccgg
     2401 gggtcgtcgt gatccaggtc gatggcctgg cgttcccgct cctggcggcc caggtccgcg
     2461 ccggcaccct ctccaacctg tcgcgatgga tccggtcagg gtcgcatcgg atgatggagt
     2521 acacggcgga cctcccgtcc cagacgtcca ccggccaggc cgggctgctc cacggcaacg
     2581 cggccgggat tcctgccttc cggtggtacg agaaggagcg gcgccgaatg atggtctcca
     2641 accgcccggc tgacgccgcc gagatcgagg ccaggatcag tgacggaaac ggtctgctgg
     2701 ccaatggcgg cgcctcgatc gacaacatgt tctccggcga cgcgacccat gtgcggatgg
     2761 tcatgagcaa gctgcacagc aacttggaca aggcaggtcg cgatcggagc atgtactact
     2821 tcctgctcaa tccgtacagc atcggtcgga ccatcgtccg gaccatcggc gaaatcggca
     2881 aggagctgta ccagggctgg cagcagcgac ggcgtgggat cgagccgcac atgcaccgcg
     2941 gcctgaagtt cgccggcctg cgagccatga ccaacgtctt cctccgtgac ctcaaccacg
     3001 ccctcgtcgc cgagcagatg ttgcgcggaa cgccggtgat gtattgcgac ttcgtggact
     3061 acgacgacat cgcccatcac gtcggacccg agcggcagga ggcgcttcag tcgctgtcgg
     3121 ggatcgaccg ctccatcggg cgcctggagc ggatggtgcc cgactgcccg cggccgtatc
     3181 ggttcgtgat cgtctccgac cacggccaga gccagggcgc gacgttccgc cagcgctacg
     3241 gcgagacgat cgaggagtgg ctgcacaagc agatcggcgc cgacgtcacc cggaccgcca
     3301 ccgccccggt cgaagaagtg ggccggatgt ccaccgtcct gaccgaggcc acctccgccg
     3361 atggcgccac cgccgccctc gggaggcgcg cgctcagggg cagcatggac gacggcgcgg
     3421 tggaactggg ggtccttggt gacgaggcgg cgaaggagcc ttctggtgag ctgcccgacc
     3481 tcgtggtctg cgcctcgggc aacctggggc tggtgtactt cggcgtcgcc gaacaccgca
     3541 tgtcgctgcc cgagatcgag gctgccttcc cgggcatgat ggacaagctc gtcgcccacg
     3601 acggtatcgg ctttgcgctc gtccgatccg gggacggcca tccgctcgcc atcggcccgg
     3661 acggccgcca tgatctctcc gatgggagcg tgaccggcac cgatccggtc gcccagttcg
     3721 gtcctgacgc accggatcag ctgcgccatc tcgacggcat cgatcacgtg ggcgacctcg
     3781 tcctgatcag ccgcctcgac acgggcaccg acgaggtcgc cgccttcgag gaactcatcg
     3841 gcagccatgg cgggatgggc gggtggcaga ctcagccgtg cctggtctat cccgcggact
     3901 ggcccgcgcc ggacggacgc ctgatgggcg ccccggccgt tcaccggcag ctgaagcgct
     3961 ggatcgccgc cgagcagggc taaggcaggc gggtgggcat cttcgacggc gtcctcggga
     4021 tcttcagcca cgatctgggg atcgatctcg ggacggcgaa cacgctggtc acggtccggc
     4081 acaggggaat cgtgatcagc gaaccctcgg tcgtggccat ggagacacgg acgaagcggg
     4141 tcctggccat cggcgccgag gccaagcgga tggtgggtcg tacgccggcc aacatcgtcg
     4201 ccattcgccc gctccgcgac ggcgtcatca gcgacttcga cgtgacggag cagatgatcc
     4261 ggtacttcat cgagaaggtg cacgaccgct atttcaggat tccgcgtccg cgggtcctgg
     4321 tcgggatccc gtcgggcgtg accgaggtcg aaaagcgggc cgtccgggac gccacgctga
     4381 atgcccatgc ccggtgggcg cggctgatcg aggagccgat ggccgccgcc atcggcgccg
     4441 gactgccggt cagcgagccg tccggatcgc tcatcgtcga catcggcgga gggacgaccg
     4501 aggttgcggt catctcgctg ggcgggatcg tggtcagccg gtcgatccgg atcggtggcg
     4561 acgagatgga tcaggacatc gtcaacttcg cgcgtcgcga gtacaacctg ctgatggggg
     4621 agcggaccgc cgaagagatc aagatcgcgg ttggatcggc ctacgccgac ctcaacccgc
     4681 ggacggtcac gttccgcggt cgggacctgc tgaccggcct gccgaggagc gtggaggtca
     4741 gcagcgagca gattcgggag gctctcgagc cgtcggtggc cgccatcatc caggccattc
     4801 gggacacgat cgaggagaca ccacccgagc tggtggccga catcatggac cagggaatcg
     4861 tgctcgccgg cggcggggcg ctgctggccg gcctcgaccg ccggacggcg gaggcgaccc
     4921 agatgccggt ccatgtcgcg gatgacccgc tgacctgcgt cgtgcgcggg accggccggg
     4981 tgctcgagga gctggacacc ctcgaacgcg tgctcatcac cgagcagtac acgcgcgccc
     5041 cgcggtaagg cggacgtgcg cggggcctcc tttcccagcg gcactccggg ggccggtccg
     5101 catcggcgaa cggggaacgg actgctgtgg gcggtgctgg tcgccgtgtc gctggggctc
     5161 gtcatcgtct cgggcaccga gccggctcga tcggtccagg agctgaccgc cggcgccctg
     5221 gctccggtcc acgccgccat cgccggcatc ggcgacggca tcgcggacgt ggtcgagacg
     5281 gtcggggcga tcgaccgcct ccgggccgag gccgaggagc tgcggagcac gctggcaggg
     5341 gccgagcagc ggattgccga gctccaggag gcggctcgcg agaacgccca gcttcggtcg
     5401 ttgctcgggc tgcaggcggt cctcggctgg gatctcgtcc ctgcccgggt ggtcgcggcc
     5461 gggaccgggg ccgtcagctg ggaggtcgcg atcgacgtcg gacgcgacga cggggttcga
     5521 accggcatgc cggtggtcgg tgccgcctcg ggcggcgggg cgctggccgg aattgtggtc
     5581 cagtcaggtt tggatcgctc cgtcgtccaa ttgaccgtgg atccccggtc gcgggtcgtg
     5641 gcccgcgacc agcagaccga tgcgctcggg gtggtccagg gtcagcccgg cggccagctg
     5701 gtcatgaccc aggtggccgt gaccgacgag gtcgccgtgg gtgacacgat catcaccgcc
     5761 ggtctggagc tcgccgaggt ggcggcgtcg cagtatccgc ggggtttgct gatcgggacg
     5821 gtctcggcgc tcgagaccga tgccaacggc ctgacccgca ccgtcttcgt tcgaccggcg
     5881 ctcgacccgc gcgccgtcga atggctcatg gtcgtccgct cggcggcccc cgaggcctcg
     5941 ccacagccct gaggtccggc cggggttccc cggctccacg cctgcctgcc gatctcggct
     6001 ggtgaccgcg cgcacatggc ccttaggtcc ggcgatgcgc ggaccatcgc cccatgggcg
     6061 tcatccgatg cggtgcgtag ggtcaattct gaactcatct gcccgttatc tgaactcatc
     6121 tgcccctcta ggagactctg ttgcacgagc gagcgctgcc tgatccttgg cggctggccc
     6181 tggtcctggg gctggcgttc gcactggccg tatcggccgt cttccatccg attcccgtgc
     6241 ggggtgccgc cgtcggccag aaggtcgtcg tcatcgtcgg cccggtgggc ggcggctcca
     6301 ttcagaccaa ctacctgaac aagggcgagg ccatcgccga cgcggccgag gcccgcggcg
     6361 ccaccgtcgt ccgggtcttc tcgccgaacg cgacctatgc cgcggcccgc gcagcggtca
     6421 gcggggcgaa tgtcatcgtc tatatcggcc acggcagcgg gttcccgaat ccctacgccg
     6481 gcaccaacca gccgaacttc aacaacggat ggggtctgaa taaggacacg agccacggcc
     6541 atcagcacgt catcggcacg tcgctggtgt actgcggcga ggcggtgctc gaagggaagg
     6601 cctcatacgc gggatgcacc gggggcaaga tcaacccggc ccccaacttc gtgatggtct
     6661 actcgaacgc ctgctatgcg ccgggcgccg gtgagaccga gcaggtggcc gtgagcagcg
     6721 aggccacggc gctcgcccgc gtttcgaact actcccgccc gatcctggcg ctgggcggca
     6781 cctacttcgc caccgacctt ggctccaagt cgctggtcga gaccatcctc gacaacccga
     6841 acgccggctg gggccagatc tacaccatgg cctcggccta cgtgtccggc gcggtgaaga
     6901 cgttcgtcca ccccaagttc tcgaccatgc aggcctggct ccagaagagc tccggccccg
     6961 gcccctcggt cagctacttc aacgcgtttg ccggtgaccc aagtcgcacg ccggccggcg
     7021 gaaccggcgc gccggtgccc gacctggcga agccaaaggt cacggcctcc tcaccgttca
     7081 ccttccagac cggtgtctcg ccgtcggccg acgtgaccgc cacgttcgac aagtcaatgg
     7141 tcggcctcga cgactggaac gggatggagc tgtacgaggc cagcgacccg ggcgacgcca
     7201 tggatgcatc ggtgagctgg gacgagggat cgcggaccgt caccctgcac ccggacagct
     7261 cactccagcc tgacacctgg tacgtggtca atttcggcga cgacgtcgag gacatctggg
     7321 gcaacccgct gggcgacgcc agctggggct tccggaccgg cggtggctcg agcccggtgc
     7381 cggcggcgac cgtgtacgga ccgagcacgc cgctgtggtt caaccccggc acctatgtcg
     7441 ggcgaaaggt cgacgcgtac ggccatgtga cctcctccaa gtccgggacg ctgtcagcca
     7501 cctcgtcggc gccgaccgcc cagaagggcg tcctgcccgg ccagagcggc acgtggtacc
     7561 tgatcacggc cggcctgtgg tccgggatgt ggatccagga gtcggccggc accgtcctgg
     7621 gcaccccgcc accgccaccg ccggcgccga tcgcgaccta cagtccgccg cagtccctgt
     7681 ggttcgcccc gggcacgtac gtcggacgcc agttcaatgc ctacggcgcc gtcgtcgcat
     7741 cgaagtcgta cacgctgggt gtcgcgtcgg cggccccgac cagcatcaag agcacggtcc
     7801 ttacccagag cggggtctgg tactacatca ccgccggcgt gtggggtggg tactgggtcc
     7861 aggagacggc tgccaccacg ctcggcacgc cgccgcctcc gccgcccggg accgaggtct
     7921 acagcccggc caagaccctg gtcttcgccg ccggcacgta tgtgggccgc aagttcgatg
     7981 ccagtggcaa cgtgacggcc accaagtcgt acaccctggc cgtctcctcg gccgccccga
     8041 ccagccagaa ggggccgatc ccgaaccaga ccggcaactg gtacctgatc acggccggcg
     8101 tgtgggccgg gtactggatc caggagtcac cgggcacgac gctcgccccg tagcgagctg
     8161 acgtcccccg tccgggaacg acggagacgg gacccggccc ggagattcgt accatggtca
     8221 ccgcaccgga tcggccggta cggtgcccag cgctgaggtc tggtgcagtc ggagggactg
     8281 cccctcgcgc tacgagagct ctcgcccgat gactcgaccg gcttccatcg tccgctccct
     8341 ggttgccacc gccctcgccg tggcaaccgt ggccggcgcg cctgctccgc tccccgtcgc
     8401 tgcctcctcg ccgccctcct cggcctggac cgttcccgcc gagctcccga acgccccgat
     8461 ccggctcgac gacggcaccg tgctcccgat gccgccggcg ggctacctca gccgcccgtc
     8521 ggtgcccgcc acgctcgatg ccgagcacgc tgacgacccg ctcgccttca ctccgggtgg
     8581 tcgaccgacg gtcgaccttg ccatcctcgg tgcctccatc gccaccgacg gcgcgccatc
     8641 ggtcttgtcg cttgggctgg ccaacccgac gaccagcgcc gcggccgacc tgacggcgct
     8701 tcccaacggg ctgaagcgcg aggtcttcgg gttcctaccg tactggatgc tcgacgccgg
     8761 aaccctccag tggatgcagt accagctcgt ctcgacgatc gcctacttcg gggtggcggc
     8821 caagccggac gggacgctgg ccacctacgc caccggctgg accgcgtgga acagctcggc
     8881 catgaccggc gtcatcaacg ccgcgcacgc gcggggcgtg aaggtcgtcc tgtcggtcac
     8941 gatgatggcc tacgacggcg gcgcccagca ggccgcgctc ctcggcagcg ccgcgaaccg
     9001 ggtgaagctg atcaacgcga tcgtggccac ggtgcgcgct cggaacgctg acggcgtgaa
     9061 cctcgacttc gagccggtcg cggtcgccca acgcgatcag tacacctcgt tcgtccggca
     9121 gctcaaggcg gccctcgtgg cggccggagc ggggtcgcgg ctgacggtct gcaccatggc
     9181 cggcgcgggg acgtgggcca ccggctacga ccttgccggt ctcgtggcgc ccggcgcggc
     9241 cgactccctg ttcgtgatgg gctacgactt cagctggtcc gggtcggccc gcgccggcgg
     9301 ggtggctccc atcgagtcgc cgtacatcct cgacgtgaac caatcggtg
//
LOCUS       DASYGU010000266         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_763953, whole genome shotgun sequence.
ACCESSION   DASYGU010000266 DASYGU010000000
VERSION     DASYGU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763953"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1348
                     /locus_tag="VGO32_07655"
     CDS             206..1348
                     /locus_tag="VGO32_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009816041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEV7200661.1"
                     /translation="MAFDMSLVSRGARLRRSPFYEAEQAYGPKGYTVYNHMLFPTNYD
                     DFEAEYWHLLNHVTLWDVAVERNLEVQGRDGFRFAQLLTPRDLSKCAVGQGKYVLITA
                     PDGGIVNDPVMLRLGENHFWFALASSDALLYAVGLAANLGWDVQIGEPDVSPLQVQGP
                     HSKDVVSALFGDDILSLKYYWFRELSLDGIPVVVTRTGWTAEVGYEIYLRDGQHGTAL
                     WERIMEAGKPFDIRPTGPSDIRRVEGGILNWGADMGLEHNPFEVGLERLCALDGNFEF
                     MGKAALGAVRENGISRKLVGVEISGDPLGMNFTKWPVTADGASVGQVTTALWSPRLEK
                     NIGYAWLPIERAADGNRASVATPDGEREATVVPMPFIDPDKAIPKS"
     gene            1345..2277
                     /locus_tag="VGO32_07660"
     CDS             1345..2277
                     /locus_tag="VGO32_07660"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEV7200662.1"
                     /translation="MSVASGIDPGLGPLAEALASGRFVVTTELRTIDSADPGAVRAAV
                     EPLRGLVDGVACTDNSGANPHLSPLAAARLVVDAGLEPILQLSCRDRNRLALQADLVG
                     AGALGIRTVLLMGGDLVTAGDHPDAKAVFDLDAGGLLEAARTLRDAGTYLSGRKVDPP
                     PRWLVGAVENPFAPPAEERPARVGSKVKSGAEFIVTQVGFDLPVLGRFCETLASRDPV
                     VRAPLLVSVFIPRSVRNLEWLRDRVAGVGVPDAVVERMAAQTADAQAAEGQRMAVEFA
                     AAVRDMPGVAGVHLIPLNDAAAAAAVATEIRRPA"
     gene            complement(2261..2722)
                     /locus_tag="VGO32_07665"
     CDS             complement(2261..2722)
                     /locus_tag="VGO32_07665"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEV7200663.1"
                     /translation="MNAVYQLLLYVHILSAITWVGGAIYAQLIALRVSRASDPAELPI
                     IARHTAYLGLRVFLPASLLIIITGVAMTLQAWSFGQTWIAIAVALWVLSAALGGIYVG
                     PRVAKVAELFEAEGPTSENARGLLNRLFLVSRLELVSFFVIVGLMVFKPGA"
     gene            2831..3304
                     /locus_tag="VGO32_07670"
     CDS             2831..3304
                     /locus_tag="VGO32_07670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7200664.1"
                     /translation="MWRRVRLEAVIDHPPERVFPYLADPARWHEFAPAVAERIAIGDR
                     TPTVGSRWAAVDRIGPFRIRFVDELAVLEIDERVVWHSSSPWNSRVEYRCLPEPRGTR
                     ILASYEGDVAGWLRLVALLPTIVLARILMRDFHGLRRRMAADQPADEPFGVRTSG"
     gene            complement(3321..>4167)
                     /locus_tag="VGO32_07675"
     CDS             complement(3321..>4167)
                     /locus_tag="VGO32_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEV7200665.1"
                     /translation="AETDPDLFWAIRGGGGNFGVATRFHFRLQEVPSVVGGILILPAS
                     ADVVAGFVAAAEAAPEELSAIANVMPAPPMPFLPEEMHGQLVVFAIMCFAGDDEAGEQ
                     AMAPFRSLATPLADMVKPMPYPEIYPPDDESYHPKAIARTLFMDRFDHQLAERIIDRL
                     AASDATMRVAQLRVLGGAMARVPADATAFAHRSRPIMANVAAFFEGPDDFAQREAWVL
                     DLAATLRQGDAAGYVNFLADEGPDAVRAAYPGATWDRLRDLKARYDPTNLFSRNQNIP
                     PATDR"
BASE COUNT          652 a         1411 c         1475 g          629 t
ORIGIN      
        1 ccgccgaaca gccacgcgtg caacgacggg atcgacaacg acggcgattt cctgattgac
       61 ctgctcgacg tcctcggttg cacgggtcca ttcgacgacg acgagagcga cttctagcga
      121 gtagtccaca ccaatcgggc gtgcgggaca tgaggcgggt cgtgtagaag aggggtccgc
      181 atcggtctgt tcgctggagg aacgcatggc tttcgacatg tccctggtct cgcgcggggc
      241 gcgccttcgc cgatccccgt tctacgaggc cgagcaggcc tacgggccaa agggctacac
      301 ggtctacaac cacatgctgt tcccgaccaa ctacgacgac ttcgaggcgg agtactggca
      361 ccttctcaac cacgtgaccc tgtgggacgt ggccgtggag cggaacctcg aggtccaggg
      421 ccgtgacggg ttccgtttcg cccagctcct gaccccgcgc gacctgtcca agtgcgcggt
      481 cggccagggc aagtacgtgc tcatcaccgc gccggacggc gggatcgtga acgacccggt
      541 catgctccgc ctgggcgaga accacttctg gttcgccctc gccagctccg acgccctcct
      601 gtatgcggtg gggctggccg cgaacctggg atgggacgtc cagatcggtg agccggacgt
      661 gtcgccgctc caggtccagg gcccgcactc caaggacgtg gtgagcgcgc tcttcggcga
      721 cgacatcctg tcgctcaagt actactggtt ccgcgagctg tcgttggacg ggatcccggt
      781 ggtcgtgacc cgcaccggct ggacggccga agtgggctac gagatctacc tccgcgacgg
      841 ccagcacggc accgccctgt gggagaggat catggaggcc ggcaagccgt tcgacatccg
      901 ccccaccggt ccgtccgaca tccggcgggt ggagggcggg atcctgaact ggggagcgga
      961 catgggcctc gagcacaacc cgttcgaggt ggggctcgag cgactgtgcg cgctggacgg
     1021 caatttcgag ttcatgggca aggcggcgct cggcgcggtc cgcgagaacg ggatcagccg
     1081 caagctggtg ggggtcgaga tcagcggcga tccgctgggc atgaacttca cgaaatggcc
     1141 ggtcaccgcc gatggcgcgt cggtcggcca ggtcacgacc gcactgtggt cgccgcgcct
     1201 cgagaagaac atcgggtacg cctggcttcc gatcgaacgt gccgcagacg gcaaccgtgc
     1261 gtcggtcgcc accccggatg gtgagcgcga agcgacggtg gtcccgatgc cgttcatcga
     1321 ccctgacaag gccatcccca agtcgtgagc gtcgcgtcgg ggatcgaccc cggcctcggt
     1381 cccctggcgg aggcactcgc cagcggccga ttcgtggtca ccaccgagct tcggacgatc
     1441 gactcggccg atccgggcgc ggtgcgcgcc gccgtggagc ccctccgcgg gctggttgac
     1501 ggggtcgcgt gcaccgataa cagcggagcg aacccgcatc tctcgccact cgccgccgct
     1561 cggctggtgg tggacgccgg gctcgaacca atccttcagc tgtcctgccg cgatcgaaac
     1621 aggctggccc tccaggccga cctcgtgggc gccggcgcac tcgggatccg caccgtgctg
     1681 ctgatgggcg gtgacctcgt caccgccggc gatcacccgg atgcgaaggc agttttcgac
     1741 ctcgatgccg gcggcctgct ggaggcagcc cggaccctcc gggatgcggg gacctacctg
     1801 agcggccgga aggtcgaccc gccgccgcgc tggctggttg gcgcggtcga gaacccattc
     1861 gcgccgccgg ccgaggagcg ccccgcgcgc gtgggctcca aggtgaagtc cggggccgag
     1921 ttcatcgtga cccaggtggg gttcgacctc cccgtcctcg gccggttctg cgaaaccctc
     1981 gcctcccgtg atccggtcgt gcgagcgccc ctgctcgtat cggtcttcat ccctcgttcg
     2041 gtgcggaacc tcgaatggct ccgagaccgg gtcgccgggg tgggcgttcc ggacgccgtc
     2101 gtcgagcgga tggccgctca gacggccgac gcccaagcag ccgagggtca gcgcatggcc
     2161 gtggagttcg cggccgccgt ccgagacatg cccggcgtgg ccggcgtcca cctgatcccg
     2221 ctcaacgacg cggccgcggc ggcggccgtg gccaccgaga tcaggcgccc ggcttgaaga
     2281 ccatcaggcc gacaatgacg aagaagctga ccagctcgag gcgcgagacg aggaagaggc
     2341 gattgaggag gccgcgagcg ttctccgacg tgggcccctc cgcttcgaac agttccgcca
     2401 ccttcgcgac ccgcgggccg acgtagatcc cgccgagagc ggcggacagg acccagagcg
     2461 caaccgcgat cgcgatccag gtctgaccga agctccacgc ctggagcgtc atggccacgc
     2521 cggtgatgat gatcaacagc gaggccggca ggaacacccg caggccgagg tacgccgtgt
     2581 ggcgggcgat gatcggcagc tcggccggat cgctggcccg gctcacgcgc agggcgatca
     2641 gctgggcgta gatggcgccg ccgacccagg tgatggcgct caggatgtga acgtagagca
     2701 ggagctggta cacggcattc accccggcgg gaatcgtccc cggcagggta gcggggaacg
     2761 cgcggttcgt gccgggtccg cgaccgggta ccatcccgcc cgtcggcgat cagcagcgga
     2821 ggcgggaacg atgtggaggc gggtcaggct cgaggccgtg atcgatcatc cgcccgagcg
     2881 ggtcttcccc tatttggcgg atccggcgcg ctggcacgag ttcgcgccgg ccgtcgcgga
     2941 acggatcgcg atcggcgacc ggacgccgac ggtcggctcc cgctgggccg ccgtcgaccg
     3001 catcggaccg ttcaggatcc gcttcgtcga tgagctggcc gtgctcgaga tcgatgaacg
     3061 ggtggtctgg cactccagct cgccctggaa ctcacgggtc gagtaccggt gccttcccga
     3121 accccgcggg acgcggatcc tggcctccta cgagggcgat gtcgccggct ggctccgcct
     3181 cgtcgcgctg ctgccgacca tcgtcctggc ccggatcctg atgcgtgact tccacgggct
     3241 ccgccgacgc atggccgccg accagccagc cgacgagcca ttcggggtgc gaacctccgg
     3301 ctgaatcggg cgggccgccg tcagcggtcc gtggcgggcg gaatgttctg gttccggctg
     3361 aacaggttgg tcgggtcgta gcgcgccttg agatcgcgga gccggtccca ggtcgcgccg
     3421 gggtacgcgg cgcgcaccgc atcgggcccc tcgtcggcca ggaagttcac gtaccccgcc
     3481 gcatctccct gacggagggt cgcggccagg tcgagcaccc acgcctcgcg ttgcgcgaag
     3541 tcgtcgggac cttcgaagaa ggccgcgacg ttggccatga tcgggcggga ccgatgggcg
     3601 aacgcggtcg cgtccgccgg gacgcgtgcc attgcgcctc ccagcacccg aagctgcgct
     3661 acgcgcatcg tggcgtccga agccgcgagg cggtcgatga tcctctcggc cagctggtgg
     3721 tcgaatcggt ccatgaacag ggtccgcgcg atcgccttcg ggtggtatga ctcatcgtca
     3781 ggcggataga tttccggata gggcatgggc ttgaccatgt ccgcgagtgg cgtggcgagc
     3841 gatcggaacg gcgccatcgc ctgctcgccg gcctcatcgt caccggcgaa gcacatgatc
     3901 gcgaacacga ccagctggcc gtgcatctcc tcaggcagga acggcatcgg gggggcaggc
     3961 atgacgttgg cgatcgccga tagctcttcg ggcgcggctt cggcagcggc cacgaacccg
     4021 gccaccacgt cggccgacgc gggaaggatg aggatgccgc ccacgaccga gggaacctcc
     4081 tggagccgga agtggaaccg ggtcgcgacc ccgaagttgc cgccgccgcc ccggatggcc
     4141 cagaacaggt cgggatcggt ctcagcg
//
LOCUS       DASYGU010000267         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1228008, whole genome shotgun sequence.
ACCESSION   DASYGU010000267 DASYGU010000000
VERSION     DASYGU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228008"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGO32_07680"
     CDS             complement(<1..218)
                     /locus_tag="VGO32_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7200666.1"
                     /translation="MSEPEGEALLEQLRATPLFAEVPIEPLKQLAAAAERLTLRAGEQ
                     LIREGDPAEELFVVVSGELEIRKQSGKA"
     gene            complement(215..1894)
                     /locus_tag="VGO32_07685"
     CDS             complement(215..1894)
                     /locus_tag="VGO32_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7200667.1"
                     /translation="MVSRPTILTVDDDLPVLRAIERDLRARYSKDYRIVAATSGTEAL
                     EAVRELTVRGDPVALFVVDQRMATMTGIEFLTEAIDLQPDARRILLTAYADTDVAIRA
                     INEIRLDQYLLKPWDPPEERLYPVLDDLLADWAAGYRPPFVGLRLIAGRWAARGHDIR
                     DYLTRNQVPYQWLDPTTDAEGRRVAVSAGVAEAEMDPAVPIVVLEDGTVLRDPTSREI
                     AEKVGIQTTAALPFYDLVIVGGGPSGLAAAVYGASEGLKTVLVEREAPGGQAGTTSRI
                     ENYLGFPAGLSGGDLARRALAQARRLGAEILSPRDVTAIRRADPYRYVRLDDGSEISC
                     HAVVIASGVSYRQLEIPGAAELAGAGVYYGAAVTEAVLYRDLEVGVVGGGNSAGQAAV
                     YLARFAKRVLLMVRTTELSSMSQYLIDQIAATPNIEVRFQTQVSACAGEGHLASATLT
                     CTTGPEPTTETLPLSALFVFIGQQPRTDWLPAEVSRDESGFVATGTAVLVDGKRPPGW
                     TPDRDPYLLEASLPGVFAAGDVRSRSVKRIASAVGEGSMAIQFVHQYLATL"
     gene            1939..3129
                     /locus_tag="VGO32_07690"
     CDS             1939..3129
                     /locus_tag="VGO32_07690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7200668.1"
                     /translation="MSAASQQADVVIVGGAVVGSAVAYFLARDPDFWGSVIVIERDPT
                     YATASTTLSAASVRQQFSTPVNIQMSQFALQVLKHPDWWFPLLETVPEFDFVEGGYLF
                     LATQAGVPTLWSNNAIQREHQVSAEILSPDELSHRFPWLNTEDIAGGSLGLAGEGWFD
                     AAAMMQAFQQSARASGVTYVTGEVTGILSFGGRIDAVMLADGRLIECGALVNAAGPRA
                     GALARFAGVDLPVVPRKRNVYSFSCKTEINDAPLVIDPTGVYFRPDAPNFIGGFSPRE
                     GEEDPDTLDLSVDLKAWEERVWPAIAHRVPAFEAVKMRRAWAGHYEVNTVDHNAIIGP
                     HPDIPNLLFANGFSGHGLQQAPAVGRGLAELVIHGAYRTIDLTPLGYERIAANRPLRE
                     LNVV"
     gene            3145..3372
                     /locus_tag="VGO32_07695"
     CDS             3145..3372
                     /locus_tag="VGO32_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200669.1"
                     /translation="MSQADSKLVSGPEPDPIAEPIPEPETEWESEGGAVLPPGLSLTL
                     RGPEGAGPEELEAALHALRRALSDGYRLERD"
     gene            3377..4045
                     /locus_tag="VGO32_07700"
     CDS             3377..4045
                     /locus_tag="VGO32_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEV7200670.1"
                     /translation="MCGRFAQPHSSEELARIFAARPTVDLPGERFNVAPTDEVAGVVE
                     HHGERLVEPFRWGLVPWYAEDRKGAARLINARAETVEKSPAFRTSFAKRRLIVPAAAF
                     YEWHRADGRRDPFAVRRRDGDPLALAGVWAVWRDPDTGERVYTCSILTTVPNELIAPI
                     HNRMPVILDPADWDLWLSEEAAVKDIRELLFPFPADTLDAFPVSSAVNNVRNEGADLL
                     ERVG"
     gene            4042..4587
                     /locus_tag="VGO32_07705"
     CDS             4042..4587
                     /locus_tag="VGO32_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7200671.1"
                     /translation="MTDFAIPDGALVLLIGAAGSGKSTLAARLFAPDEVLSSDAFREA
                     VSGDSRDQTATEEAFDRLHADLDLRLAAGRLSVVDATNVQGWARRSLLAVAARHGRPA
                     VAVVLALPLDVSLAQNAARTSGRVPAGVVRRHDRDLRTSLPRLVEEGYATVVVLTVPG
                     QVEALRIRRQRNVPKERHPTL"
BASE COUNT          681 a         1641 c         1620 g          714 t
ORIGIN      
        1 tccgccttgc ccgactgctt gcggatctcg agctcgccgc tgaccaccac gaagagctcc
       61 tcggccggat cgccctcccg gatgagctgc tcgccggcgc gaagggtgag ccgctcggcg
      121 gccgccgcca gctgcttgag cggctcgatc gggacctcgg cgaacagcgg cgtggctcgg
      181 agttgctcga ggagggcctc gccctcgggc tcgctcacag cgtggccagg tactggtgca
      241 cgaactggat ggccatcgag ccctcgccga ccgcgctcgc gatgcgcttc accgatcggg
      301 accggacatc cccggccgcg aacacgcccg gcaggctcgc ctcgaggagg tacgggtcgc
      361 ggtcgggcgt ccagcccggg gggcgcttgc cgtcgaccag cacggcggtc ccggtcgcga
      421 caaacccgct ctcgtctcgg ctgacctcgg ccggaagcca gtcggtgcgt ggctgctgcc
      481 cgatgaacac gaacagtgcc gacagcggca aggtctccgt tgtgggctcc gggcccgtgg
      541 tgcaggtgag tgtcgcgctc gcgaggtggc cttccccggc gcaagcgctg acctgggtct
      601 ggaagcgaac ctcgatgttc ggcgtggccg cgatctggtc gatcaggtac tggctcatcg
      661 aggacagctc ggtggtgcgg accatgagca ggacacgctt cgcgaaccgg gcgaggtaga
      721 ccgccgcctg gccggccgag ttgccgccgc cgaccacccc gacctcgagg tcgcggtaca
      781 ggaccgcctc ggtcacggcc gctccgtagt acacgccggc acccgccagc tccgcggcgc
      841 cggggatctc gagctgccgg tagctcacac cgctcgcgat cacgaccgcg tggcagctga
      901 tctccgaccc gtcgtccagg cggacatacc gatacgggtc tgcgcggcgg atggccgtca
      961 cgtcccgcgg agacaggatc tcagccccca gccgacgggc ctgggccagc gcccggcggg
     1021 cgagatcccc gcccgacagg ccggccggga agccgaggta gttctcgatc cggctcgtgg
     1081 ttcccgcctg cccaccgggc gcctcgcgct cgacgagaac cgtcttcagc ccctccgacg
     1141 ccccgtagac cgcggccgcc agcccggatg gtcccccgcc cacgatgacg aggtcataga
     1201 acggcagcgc ggcggtggtc tggatgccga ccttctcggc gatctcgcgg gacgtaggat
     1261 cacggaggac ggtcccgtcc tcgaggacca cgatcgggac ggcgggatcc atttccgcct
     1321 ccgccacccc ggcgctcacc gcgacccgtc gtccttcagc gtcggtcgtc ggatcgagcc
     1381 actggtaggg aacctggttg cgggtcaggt agtcgcggat gtcgtggccc cgtgcggccc
     1441 accgaccagc aatcagtcga aggccgacga acggcggccg atagcccgct gcccagtcgg
     1501 ccagcagatc gtcaagaacc gggtacaggc gctcctcggg cgggtcccac ggcttgagca
     1561 ggtactggtc gagccggatc tcgttgatgg cccggatggc gacatcggta tcggcatacg
     1621 cggtgagcag gatccgacgg gcgtccggct ggaggtcgat cgcctccgtc agaaactcga
     1681 tcccggtcat cgtcgccatc cgctggtcca cgacgaacag ggcaaccggg tcgccgcgga
     1741 cggtcagctc gcgaaccgcc tcgagcgcct ccgtcccgct ggtggcggcc acgatccggt
     1801 agtccttcga ataccgcgcc cggaggtcgc gctcgatggc gcgcagaacc ggcaggtcgt
     1861 catcgacggt gaggatggtg gggcggctga ccacggggtg agagtctagc cttggatgcg
     1921 caggctagga ttcggccgat gagtgccgca tcccagcagg ccgacgtggt gatcgtgggc
     1981 ggagccgtcg tcggctcggc ggtggcctac ttccttgccc gtgaccccga cttctgggga
     2041 tcggtgatcg tcatcgagcg cgacccgacc tatgcgacgg cttcgaccac cctgtcggcc
     2101 gcatcggtcc gccagcagtt ctcgaccccg gtcaacatcc agatgagcca gttcgcgctg
     2161 caggtcctga aacaccccga ctggtggttc ccgctgctcg agaccgtgcc cgaattcgac
     2221 ttcgtggagg gcggctacct gttcctcgcc acccaggccg gcgtaccaac cctgtggtcc
     2281 aacaacgcga tccagcgcga acaccaggtt tcggcggaga tcctgtcccc cgacgagctg
     2341 tcgcaccggt tcccctggct caacaccgag gacatcgccg gaggctcgct gggcctggcc
     2401 ggggagggct ggttcgatgc cgcggccatg atgcaggcct tccagcagtc ggcccgtgca
     2461 tcgggcgtga cctatgtcac cggtgaggtg accggcatcc tgtccttcgg cggtcggatc
     2521 gatgcggtga tgctggccga tggccggctc atcgagtgcg gggccctggt caacgccgcc
     2581 ggtccgcggg ccggggcgct ggcccgattc gccggggtgg atctgccggt cgtgccccgc
     2641 aagcgaaacg tgtactcatt ctcctgcaag accgagatca acgacgcgcc gctggtgatc
     2701 gaccccaccg gcgtctactt ccgccccgac gccccgaact tcatcggcgg cttctcccca
     2761 cgagagggcg aggaggaccc cgacaccctg gacctgtcgg tggacctcaa ggcctgggag
     2821 gagcgggtct ggcccgccat cgcccaccgc gtaccggcgt tcgaagcggt gaagatgcgg
     2881 cgggcatggg ccgggcacta cgaggtcaac acggtcgacc acaacgcgat catcggcccg
     2941 cacccggaca tcccgaacct gcttttcgcc aacgggttct ccggtcatgg cctccagcag
     3001 gcgccggcgg tcgggcgggg gctcgccgag ctggtcatcc acggcgcgta tcggaccatc
     3061 gacctcacgc cgcttggcta cgagcggatc gcggccaacc gacccctccg tgagctgaac
     3121 gtggtctagc ggtaggctgg gcacatgtca caggccgatt ccaagctggt gtccggacct
     3181 gagccggacc cgatcgccga accgatcccc gagcccgaaa ccgagtggga gtcggaaggc
     3241 ggagccgtcc tgccgccggg cctgtccctg accctgcgcg gtcccgaggg agcgggaccc
     3301 gaagagctgg aggccgccct ccatgccctg cgccgggcgc tctccgacgg ataccggctg
     3361 gagcgcgact gacaccatgt gcggacggtt cgcgcaaccg cactcgagcg aggagctggc
     3421 ccgcatcttc gccgcgcggc caaccgtcga cctccccggc gagcgcttca acgtcgcccc
     3481 caccgacgag gtcgcgggcg tggtcgagca ccacggggag cggctcgtgg agccgttccg
     3541 ctgggggttg gtgccgtggt acgcggagga tcggaagggt gccgcccggc tcatcaacgc
     3601 gcgggccgag acggtcgaga agtcgccggc gttccggacg tcgttcgcca agcgccggct
     3661 gatcgtcccc gccgccgcgt tttacgagtg gcaccgcgcc gacggacggc gagacccgtt
     3721 cgcggtccgc cgccgcgatg gcgatcccct ggccctggcc ggcgtgtggg ccgtctggcg
     3781 cgaccccgac accggagagc gggtctacac ctgttccatc ctgaccaccg tcccgaacga
     3841 gttgatcgcg cccatccaca accggatgcc ggtcatcctc gacccggcgg actgggacct
     3901 gtggctgtcg gaggaggcgg cggtcaagga catccgcgag ctgctgttcc cgttccccgc
     3961 cgatacgctg gacgccttcc cggtctcgtc ggcggtcaac aacgtgcgca acgagggagc
     4021 cgacctgctg gaacgggtgg gttgaccgat ttcgccatcc cggacggagc gctggtcctg
     4081 ctgatcggtg cggcgggcag cggcaagtcc acgttggccg cccggctgtt cgcaccggac
     4141 gaggtgctct cgtcggacgc gtttcgagag gccgtgtccg gggactcccg ggaccagacg
     4201 gccaccgagg aggcgttcga ccggctccat gccgatctcg accttcggct tgcggccggc
     4261 cggctgtccg tggtggatgc gaccaacgtc cagggctggg cgcgtcggtc gctgctcgcg
     4321 gtggccgctc gccatgggcg ccccgcggtg gcggtggtgc tggcgctgcc cctcgacgtg
     4381 agcctggcgc agaacgcggc acggacctca gggcgtgtgc cggctggggt tgtgcggcgc
     4441 catgaccgcg atctccggac gtcgctcccg cgcctcgtcg aagaggggta tgcgacggtg
     4501 gtggtgctca cggtcccggg ccaggtggag gcgctcagga tccggaggca acgaaatgtc
     4561 ccgaaggaac ggcacccgac cctttgatgc aatctccgga gccgtcaccg cgtccttgat
     4621 gcaatctcag acccggatcg tgctgcccct tcggga
//
LOCUS       DASYGU010000268        15755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_250047, whole genome shotgun sequence.
ACCESSION   DASYGU010000268 DASYGU010000000
VERSION     DASYGU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15755
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250047"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VGO32_07710"
     CDS             complement(<1..1550)
                     /locus_tag="VGO32_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200672.1"
                     /translation="MIRPRGSDLLLLTAAALIARIAAAVLVDYSPYTDPAYYAMVAEQ
                     LATGHGFSAPVLWSFLEVGGSLPADPMLPVPSNGHWMPLTSIVAAAGMALLGPDLRAG
                     QVPIIALGVALVPFTYLVGLDLYESRRVAILAALLAVFAGPLLIMYPTVDNFAVFGAA
                     GAVALWAAIRATRPGRATWWLVISGAAVGLATLARVDGLLLGMAPLTAWLVDRGMGPW
                     TGGGARRMGVAALLLAGVAGLVVMAPWLARNMATFGSAFPSAGGHTLWITSYNEQFSI
                     SANPTPASYLAWGWANIIGSKLSTWWELVWRSVVLLGGIFGFLFFAGLWRERRRPDRA
                     PFTVYWLVMFFTMGAVFTFHAPHGAYYHSAAAWLPFALPMAVASTGPVLTALGRWWAF
                     LRRPPTHRFVEVVALGGAVVLSVIGSITLFDLWDYSHGREQAAAAYLEEAGLTDDPVL
                     YGDPSSLWHLTGNPGVPAPFDPYAVIEEVVRAYDIRWVIVTLREGGTTDPLGLWNGCA
                     ARDSVDNSA"
     gene            complement(2327..3220)
                     /locus_tag="VGO32_07715"
     CDS             complement(2327..3220)
                     /locus_tag="VGO32_07715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV7200673.1"
                     /translation="MFGFSVENPRTQRALQAAALGLGIAFALMSIHGALFWTDGQAYW
                     MAAERLRAGEELYSATISQDAAEVYRYAPWFAYAWIPLTYLPEEYVAMGWTLVMLAAW
                     IFPVSAFLALGWRERSVAFLVAPPLLVAALGGNVQPAVIALLYAGLERRWGPGAIGVA
                     ASLKLFPILFVAVYVARRQWVAAAAAVGLGGFLWLPALASDIRHYPIEVGGAFSLLGM
                     SPVVYAVATAAAVGWSLRSASWSAAGVAVVIGSCVRFIPYQLGYLLCSRPLPVEVRVE
                     ADVPLRTRAAPSGSSTATSTG"
     gene            3306..4199
                     /locus_tag="VGO32_07720"
     CDS             3306..4199
                     /locus_tag="VGO32_07720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV7200674.1"
                     /translation="MQRSRVGWGVLIGLYLALGIAGAVYLAPTTVIGQDLATYQRAGE
                     LLWSGENPYSGQSDLGQENQYRYPPLLAMVIPVLGWPPLWYGLLAVATAATIWLWYRT
                     AGLPGLLVPALLVGAWGQQLLNGNSQAIVVWLLAITPLVGGPRSGWRGSMALGPIALA
                     VATMLKIHPVIGLVWFAGRRDWRAIGVYAAACAVLLLIQLPWFGDFIDYYLNDPAAAE
                     TIPGMSLRAIHPAVWIVGFLVVLALAWRYAGTRYGWLLATIAQLVALPRVLLVNLALL
                     LAAPLPPRQRDEGAGTSPPST"
     gene            complement(4117..5061)
                     /locus_tag="VGO32_07725"
     CDS             complement(4117..5061)
                     /locus_tag="VGO32_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200675.1"
                     /translation="MTGLASVRSMPAVVDGALRWSLLIAIGFLAAVNVFMASLQIAEI
                     LLGSDALDWQTYVLAGERFWTGTLYEQAESWYGWRYSPLAALPFVALAPLGETIWRVL
                     LLASLLALPRRLIFLALASYPLWFAIHAGSLMVPVAVAAFWALRGRWWAVGAFLVLAL
                     LIPRPLMVPIVIWILWKEPRWRIPFGVLFLVHLGLVVASGYLDDWMQALISTGGEEIG
                     SVFNVAPSAIIGPTWLLLAIPLALWAFRARRPALAGLLIQPYWLPYYLLILLADPLPS
                     RSWWRDHLPLTFTSKVGWFRLPHRADEGAAAPQAAAPG"
     gene            complement(5058..6104)
                     /locus_tag="VGO32_07730"
     CDS             complement(5058..6104)
                     /locus_tag="VGO32_07730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV7200676.1"
                     /translation="MTASTGRTVSGRSLSQTLRRAWLSDTSLIIFAAVAFLLADAVIV
                     YIAARGDFAIDFTCCYQQAGQRLLDDPATLYSWTDTYLFRYSPWAAVLFAPLASLSET
                     AAIWAWFVIKAVVVVGVAAGLARRWTGDARWLIAGMVLFFPPIWHDLALGNVSVFTVA
                     VLAVLLARRDGVGGVAFGLLLVLAPKPHLLPLAIWLAVRRPRDAVAAFATLALGVAAG
                     ILVFGPDLWLDWLATFREPLERTFTANIGFSGLLGPAGVVVGVVAAAVVGGAAFVRRG
                     DIGLGLSVVAGIVLGPYSFIHYLSGLVVVIDPVLRRRPRWLAPYPWLLIVFPLIPVWL
                     LALAWTMWRTPVDT"
     gene            6206..7681
                     /locus_tag="VGO32_07735"
     CDS             6206..7681
                     /locus_tag="VGO32_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200677.1"
                     /translation="MLRLPSTAAVFAAGLAGIMVVIVLAKGLTDPDFFTHVRTGQLIV
                     DTGQVPTADPFSFTWFGQPWTLHEWLSEVLMYLLISGPGVMLSLITFGLIPGALLAVL
                     LVSVLKRGVPLRPAALACILAAWVFIPYVTIRPQAISWLLMAVLVAFLWGLDAAKPRR
                     ALWLIPLFILWANVHGLWVVGLGVVALYGLFTVAGRTPMAGAGGRQWIGLAFVGCLLG
                     VMLTPAGPAGVLYPLRYIDAGDWGLANIHEWQSPDFHGAAHLGLLTLILTLLAVGIWG
                     GAPGWMSVLTILGVAMSLISLRNAPLLAVWSVPVVAMGLAARWPARLTARPVPASQEP
                     VRRAMEAIAAVAVVVISAVLVLPQTPAVHLEETVASEFPEAAMEIIREVNPDARVLAE
                     YGWGGYVIWSGYDHGVRVFVDGRNDMYDQAILEDYSAIRAADPGWEQLLAEYEVEAMI
                     WPPEAVLTRGLLDGPLADGTEWCEVFRDETQILYLPECLPD"
     gene            7666..9261
                     /locus_tag="VGO32_07740"
     CDS             7666..9261
                     /locus_tag="VGO32_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200678.1"
                     /translation="MPAGLTRGGPSPARIPWLLIGLVTLVGLAVRAIPVALADFPVND
                     GGLFLAMTRAIQDAGWALPATVAWNGNALPFAYPPLAFYGAGALEAVLGIDLLTVFRW
                     MPLVASTLIVPAVFLLGRAVLRSDLGGVVAALAYALAPASYVWMIQGGGITRSPGLLL
                     AVLAVWQSVFLVREPTRRRTVVVGLLAGMTALIHPAAALFAAISGVLLLVFEGRSRES
                     LAHATAALGVAALVALPWAAIVISRHGFAALTDVPSNGPDPVTALLAVFAGRVTGIPF
                     VSPLALVGAALVVLALLRRQWFLPLWFAASLVLSYQYAMIPFGMLIGVLVVDLVALRA
                     RDHEPAAADKSVRWIPVFGVVALATCFVIEGVGSAATVLNPSAPVHALSADRRLAMAE
                     VAAMPDGTFAVITGSEWSGDPDSEWFPLLADHPSVATVQGAEWLGQEAFEDQVIAYRA
                     LQACVPTATSSCVSEWLDLWPADYLYLPNGRLHGPNSPPDCCTALREALRTDPAFTVI
                     YDESGGTIFVDSELTRSEPAAVR"
     gene            9469..10761
                     /locus_tag="VGO32_07745"
     CDS             9469..10761
                     /locus_tag="VGO32_07745"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7200679.1"
                     /translation="MAERRIAVLGAGALGLTAALRLTQRGDRVTLFEREPVPGGLAAG
                     FQPAGSDGPWLEKFYHHLFQSDHHAIALINELGLGSDLSWHRPRTVVLRDGALHQLDS
                     PMSLLRFRPLPVMDRIRMGLALAYLKTLRSPRRLEGARAAPWIRGQMGDRAAEVVWDP
                     LLRAKFGDAAETIALPWFWARVHDRTTRLGYLGGGFQRLYNELADRIAAGGGVLHLGR
                     DVTRIERSGDRLSVTHGAGDGVATEEFDLVVSTLPTRITARLTPEMPADWHERHDPGA
                     ALGAHCLILALDRPLTDAYWINLNDPGFPFLALVEHTNMRSPAEYGGQHLIYLGSYRP
                     MDDPVLKTDADTLLTDWLPALKRLNPDVDRSWITGVWGFAAPFAQPIVTVEYASRIPP
                     FQTPVAGLWIANMFQVYPHDRGQNYSIDLAERLVAALG"
     gene            complement(10775..12244)
                     /locus_tag="VGO32_07750"
     CDS             complement(10775..12244)
                     /locus_tag="VGO32_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7200680.1"
                     /translation="MRSALPLLLVVILGGVVRAVPFMGSDFPLHDGGMFSVMASELLA
                     SGFTIPAETSFNDAGIPWVYPPLGVAIAAIGTAMFQMEVVFRVLPFLYAVASVGVFYA
                     LARALRLPGHPAIAALAFALIPHAYDWLITGGGVTRGLGLVLAMGAVTSAVHLIRSPG
                     WLRLVLTGVLGGLAVMTHPEAGAFAGLSVLVILIFEARRLPAVPWLVASGLVALVVAA
                     PWWLNVVATHGIGILVDAGGTRFGLGALLIRLTSLRYTGAAHLLLDVFLLLGLVGTLH
                     EIRQGRWILPVWFLAIVAALPRGSDVYMTVPLALMAAAAIEGVGIPLARANPRTGKAL
                     AGAVFAVALFGALSVPLNPAAPVDQIPRSDRQVMEELRTSGLTEVAVLTGRPWWGEGP
                     GEWFPAISGIRNPTLTQGSEWLGAEVWQQTQLLNYELQRCSRISAECLRTWMVEHGIG
                     HVFVSAGLGEEAFRSDLAADEGLTTIRENGRTLLVELAD"
     gene            complement(12253..13290)
                     /locus_tag="VGO32_07755"
     CDS             complement(12253..13290)
                     /locus_tag="VGO32_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV7200681.1"
                     /translation="MSVLSTSLRGVIAVASASRPIKIVGFIALMAIGALSLRISITNA
                     LVYVEWADAISYAEGARRVYAGLTPYTDMQLAGPYPLDAAIYGLGFVYPPSGAYLLAP
                     FTLGEPFFYLWVALSFVVVIGIVLLMVRREVGRMSAAMAIAVVAITATAFQVGFSELK
                     SGYVSPMVAAAMGSMWLWPRWSAIPALLCGLIKAFPAAGVLWAIRTKGVWKGPLLVAV
                     AMALLLAIAHPGWIADWLVALGNAEAACPPYALWSVACVGLPVVVGYVAGLLLLLASW
                     LSKRDDVSFLLLGLAMTVPLPDIYWGNLMLPMIAGIPLVIHESRRWLHQRQSDAPAVK
                     PVSDEIVRAHT"
     gene            13337..14257
                     /locus_tag="VGO32_07760"
     CDS             13337..14257
                     /locus_tag="VGO32_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV7200682.1"
                     /translation="MHHWRTRREWPLILAIAVAACTFGRPPVDGAGPGSASPSVEEFP
                     VILAAGDIGACDSNGDEATAALLEEHDGAILALGDLAYPAGRADDFTKCYAPSWGPDR
                     DRTHPVPGNHEYATLRAKPYYDYFGAAAGDPQEGWYAFDLAEWHVIALNSNCWEIGGC
                     SADSRQAEWLRADLADHSSACTLAFWHHPRWSSGEVHGSDGATTTFWEVLHDAGGDIV
                     LAGHDHLYERFEPMDRDGVAEPDGMVEFVVGTGGKSLFGFADILPTSAAHDNSSFGVL
                     KLTLRPGAYDWEFLSTSGTGFTDAGTTACR"
     gene            complement(14188..15084)
                     /locus_tag="VGO32_07765"
     CDS             complement(14188..15084)
                     /locus_tag="VGO32_07765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV7200683.1"
                     /translation="MTSASNGNVARFDRWAALRHGLTIAGLVYIAAVWLRIVPYAEPV
                     PDYGPMFDAYGIWNAWSGGLYDIPWLEYEAYVYSPAFAQLIYPFTLLPWPVFAALWTG
                     LAIGILFWMRVPWMLAFPGVIDDILRGNIHVFLAGAVVLAVWRQPWGAGAWAFPFITK
                     VTPGIGILWHPLRGEWRAFAVGIGVTGAIVGLSVLTVPQLWAEWFALLAANVGSDPRI
                     QVIPLPFLVRLPIAVVVIVVAARTDRAWLLPIGVMLALPNVWTSSLALLAGSAAFWWA
                     QRQAVVPASVKPVPEVERNSQS"
     gene            complement(15097..15504)
                     /locus_tag="VGO32_07770"
     CDS             complement(15097..15504)
                     /locus_tag="VGO32_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200684.1"
                     /translation="MADPEAAAWPKAIDAITLFVEDLDAARTFYGKVFGLPVMFEDAD
                     SAVFNFGNTVINLLRVSAATSLIEPAVVASADAGSRMQFTIEVDDVDAMCTTLHERGV
                     ELLNGPMDRPWGIRTASFCDPGGHIWEIAQNRR"
     gene            complement(15518..15673)
                     /locus_tag="VGO32_07775"
     CDS             complement(15518..15673)
                     /locus_tag="VGO32_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200685.1"
                     /translation="MRFLAAVVLVVVLLVGAFKLSGMQLPILDYPLGGPMSQPRIEID
                     PNPDLFP"
BASE COUNT         2657 a         5466 c         5285 g         2347 t
ORIGIN      
        1 gcgctgttgt ccacgctgtc acgcgctgcg cagccgttcc acaggcccag cgggtcggtg
       61 gtgccgccct cgcgcagggt gacgatcacc cagcggatgt cgtaggcgcg gacgacttcc
      121 tcgattaccg cgtaaggatc gaacggagcg ggcacgccgg gatttccggt caggtgccac
      181 agggacgatg ggtcaccgta caggaccggg tcatcggtca ggccagcctc ctccaggtag
      241 gcggcggccg cctgctcacg gccgtgggag tagtcccaca ggtcgaacag cgtgattgag
      301 ccgatgaccg acaggacgac ggcgccaccg agggcaacga cctccacgaa ccgatgggtc
      361 ggcgggcgtc ggaggaaggc ccaccatcgg cccagcgcgg tgagcaccgg gccggtggag
      421 gcgaccgcca tgggtagcgc gaatggcagc caagccgccg cggagtggta gtacgcccca
      481 tgcggggcat ggaaggtgaa caccgccccc atggtgaaga acatcacgag ccagtagacg
      541 gtgaatggcg cgcggtccgg gcggcgacgc tcgcgccaca ggccggcgaa gaagaggaag
      601 ccgaagatcc cgccaaggag gacgaccgac ctccagacga gctcccacca ggtcgaaagc
      661 ttggagccga tgatgttggc ccatccccag gcgaggtagc tcgccggggt ggggttcgcg
      721 ctgatcgaaa actgctcgtt gtagctcgtg atccacaggg tgtggccgcc cgctgagggg
      781 aacgccgagc cgaacgtcgc catgttgcgg gcaagccacg gtgccatcac caccagccca
      841 gccaccccgg ccagcagcag ggccgcgacg cccatccggc gcgcgccgcc accggtccac
      901 ggtcccatcc cacggtccac caaccaggcg gtcagcgggg ccatgcccag caggagtccg
      961 tccacccggg cgagcgtcgc cagcccaacg gccgcgcccg agatcacgag ccaccacgtc
     1021 gcgcggccgg gccgagtggc acggatggcg gcccagaggg cgaccgcgcc ggccgcgccg
     1081 aacacggcga agttgtcgac ggtgggatac atgatcagga gcggcccggc gaacacggcc
     1141 agcagggcgg ccaggatcgc cacccgccgc gactcgtaca ggtcgagccc gaccaggtac
     1201 gtgaacggca cgagcgccac gcccagcgcg atgatcggaa cctggccggc gcgcaggtcc
     1261 ggcccgagga gggccatccc ggccgcggcc acgatcgagg tcagcggcat ccagtgcccg
     1321 ttgctcggga cgggcagcat gggatcggcc gggaggctgc cgcccacctc gaggaacgac
     1381 cacaagacgg gcgcgctgaa gccgtggccg gtcgcgagct gttccgcgac catggcgtag
     1441 taggctggat cggtataggg ggagtagtcg accaggacag cggccgcgat ccgcgcgatc
     1501 agcgccgccg cggtcagcag cagcaggtcg gagccgcggg gccgaatcat gggcgtggcg
     1561 cgcgcgacat cgcgggtgag tctatcgtgg catggcggcc cacacggcct gccatccctg
     1621 cccctcgccg cgcttctcgg gaccgccgcc gcgctcagcc tgtggtcgat catccccctc
     1681 gtcgaggcgg tgttcaccgg ctactacgcc tttgactggg agatcttctc ggtcgccgcg
     1741 gagcgtctcg cgaacgggac gctctacgac ttcagcgagt tctacgcgtt ccgctggtcc
     1801 ccggtggcgg cctggatcct gggcttcatc acgctcatgc cgctgtggct gtggcaggtc
     1861 ctccacgtcg cggtcctgcc actcctccgc tcgtggtggc tggtcgcggc gtgcctgatc
     1921 acgttcccgc tctggttcga catccagacc ggcaacctca tgatcttcat cgcggtcacc
     1981 gggttctggg cggtgcgcgg gaaccgggtg gcgatcggcc tgttcctcgt gctcaccctg
     2041 ctcgtaccgc gcccgctcat gctgccgctc gcggcctgga tcctgtggac gcgaccggcc
     2101 tcgcgcatgc cattcctgct gctgttcgtg gcgcacgcga ccatcgtgat cgcgagcggg
     2161 tacgcggcgg aatggctcga tgcgctgttt cgagtgggac ccgagctcac gagcgactac
     2221 aactacggcc cctcgcgggt catcggcctg tggtgggtcc ccatcggtgt cgcattggcg
     2281 gtgtggctca cccggcgcgg tcggatcggt ctggccagcc tggtcatcag cccgtactgg
     2341 ttgccgtact acttcctgat ggtgctgctc gagttcgcag cgggacgtcc gcctccaccc
     2401 gcacctcaac cggcagcggt cgcgagcaga gcaggtatcc cagctgatac gggatgaacc
     2461 ggacgcagga gccgatgacc accgccacgc cggcggcgct ccagctcgcg gatcgcaggg
     2521 accagccaac cgccgccgcg gtagcgacgg cgtagaccac gggtgacatg ccgaggaggc
     2581 tgaaggcgcc gccgacctcg atcgggtagt ggcggatgtc gctggccagc gccggcagcc
     2641 acaggaagcc gcccaatccg acggcggcgg ccgcggccac ccattggcga cgggcgacat
     2701 acaccgcgac gaagaggatc gggaacagct tcaggctggc ggccacgccg atcgcacccg
     2761 ggccccagcg ccgttccagc cccgcgtaca gcagcgcgat cacggcgggc tggacattcc
     2821 cccccagcgc ggccaccagg agcggcgggg cgacgaggaa ggcgaccgat cgctcgcgcc
     2881 aacccagggc caggaacgcc gaaaccggga agatccaggc agcgagcatc acgagcgtcc
     2941 agcccatggc gacgtactcc tcgggaaggt acgtcagcgg aatccaggca tacgcgaacc
     3001 acggcgcata tcgatacacc tccgccgcgt cctggctgat ggtggccgaa tagagctcct
     3061 ccccggctcg gagccgctcc gccgccatcc agtacgcctg accgtccgtc cagaacagag
     3121 cgccatggat gctcatcagc gcgaacgcga tcccgagtcc aagggcagcg gcctgcagcg
     3181 ccctctgtgt tcgcgggttt tcgacgctga acccgaacat ggcgggagtc ttgggtgcgc
     3241 gcgattcgcc ctcaacggcc cattcgtacc gcggtccggc agacctccgg tgatagcctc
     3301 gcccggtgca gcggtcacgg gtgggctggg gagtcctcat cggtctgtac ctcgccctcg
     3361 gcatcgccgg tgccgtctac ctggcgccga ccacggtcat cggccaggac ctggcgacct
     3421 accaacgcgc gggggagctg ctgtggtccg gcgagaaccc ctactcgggg cagtcggacc
     3481 tgggacagga gaaccagtat cggtatccgc cgttgctggc catggtcatc ccggtcctgg
     3541 gctggccgcc gctctggtac ggcctcctcg cggtggcaac cgcggccacg atctggctgt
     3601 ggtaccggac ggcgggactg cccggcctcc tggtcccggc cctcctggtc ggggcgtggg
     3661 gtcagcagct gctgaatgga aactcgcagg cgatcgtggt ctggctgctg gccataacgc
     3721 cattggtcgg tgggccacgt tcgggctggc gggggtccat ggcgctcgga ccgatcgcgc
     3781 tcgcggtcgc caccatgctg aagatccacc cggtcatcgg cctggtctgg tttgctggcc
     3841 gtcgcgactg gcgtgcgatc ggtgtgtacg cggccgcctg tgccgtcctc ctgctgatcc
     3901 agcttccgtg gttcggcgac ttcatcgact actacctgaa cgacccggct gccgccgaga
     3961 ccatccccgg catgagcctg cgcgccatcc acccggccgt gtggatcgtc ggcttcctgg
     4021 ttgtgctcgc cctggcgtgg cgctacgccg gcacgcggta cgggtggctg ctggccacca
     4081 tcgcccagct ggtggccctg ccgcgggtcc tcctcgtcaa cctggcgctg ctgcttgcgg
     4141 cgccgctgcc ccctcgtcag cgcgatgagg gagccggaac cagcccacct tcgacgtgaa
     4201 cgtcagaggc aggtgatctc gccaccatga gcgggaaggg agtggatcgg ccaggagaat
     4261 cagcaggtag tacgggagcc agtagggctg gatgagaagc ccggccaggg cgggtcggcg
     4321 tgcccggaag gcccacagcg ccagcggaat ggcgagcagg agccatgtgg gaccgatgat
     4381 cgcggagggg gcgacgttga acacgcttcc gatttcctct ccgccggtcg aaatgagcgc
     4441 ctgcatccag tcgtcgaggt acccggacgc gacgaccaga ccgaggtgga cgaggaacag
     4501 gacaccaaac gggatccgcc aacggggttc cttccacagt atccagatga cgatcggcac
     4561 catcaacgga cgggggatga gaagggcgag aacgaggaac gcgccgacag cccaccaccg
     4621 accgcgaaga gcccagaacg cggccacagc gacgggaacc atgaggctcc cggcatggat
     4681 cgcgaaccag agcggatatg aggcgagcgc aaggaagatg aggcgtcgcg gcagggccag
     4741 gagcgacgcc aggaggagga cgcgccagat ggtctcgccc aggggagcca gcgcgacgaa
     4801 cggcaaggcc gccaggggcg agtatcgcca cccgtaccac gactcggcct gctcgtagag
     4861 ggtgcccgtc cagaaccgtt cgccggccag cacgtaggtc tgccaatcca gggcgtccga
     4921 gccgaggagg atctccgcga tctggagcga cgccatgaac acgttcaccg cggcgaggaa
     4981 cccgatggca atgagaagcg accatcgcag cgcgccgtcc acaacggcgg gcatcgatcg
     5041 aacagaggcc agaccggtca cgtgtccacc ggtgtgcgcc acatcgtcca ggccagcgcc
     5101 agcagccaca ccggaatgag cgggaagacg atcaggagcc acgggtaggg cgcaagccat
     5161 cgcggacggc gacggaggac cgggtcgatc accaccacca gccccgacag gtagtggatg
     5221 aaggaatagg gcccgagcac gatcccggcc acgaccgaca ggcccagacc gatgtcgccg
     5281 cgtcgaacga aggcggcgcc tccgacgacc gctgccgcca ccacgcccac caccacgccg
     5341 gccggcccga gcaggccgct gaagccgatg ttggcggtga acgttcgctc aaggggctcc
     5401 cggaaggtgg cgagccagtc caaccacagg tcaggaccga ataccaggat cccggccgcg
     5461 acgcccaggg ccagagtggc gaaggcagct acggcatcgc gtggtcgccg cacggccagc
     5521 caaatggcga gcggcagcag atgcggcttg ggcgcgagga cgagcagcag cccgaaggcc
     5581 acgccaccga cgccgtcgcg tcgcgccagg aggacggcca gcacggcgac cgtgaacacg
     5641 ctcacgttgc ccagggccag gtcatgccag atcggcggga agaagaggac catgcccgcg
     5701 attagccatc gcgcgtcccc ggtccatcga cgggcgagcc ccgctgccac ccccaccacc
     5761 acgactgcct tgatcacgaa ccacgcccag atcgcggccg tctcggacag cgaggcgagc
     5821 ggcgcgaaca ggaccgcggc ccagggcgag tagcggaaca ggtaggtatc ggtccaggag
     5881 tagagggttg cggggtcgtc gaggaggcgc tgccccgcct gctggtagca gcacgtgaag
     5941 tcgatcgcga agtcgccgcg ggccgcgatg tagacgatca ccgcgtcggc caggaggaat
     6001 gcgaccgcgg caaagatgat caggctggtg tccgacagcc aggcccggcg cagggtctgg
     6061 ctcaggcttc gcccgctgac ggtgcgcccg gtggaggcgg tcacccggcc atgctgccac
     6121 gcgccccacc gggtggggag cggcgaagcg gatgagtacc ttcgggtcat ctcggtgcgt
     6181 ggaggcaccg attagactcg cccacgtgct gaggctcccc tccaccgcgg ccgtgttcgc
     6241 ggccgggctg gccgggatca tggtcgtcat cgtgctggcc aagggcctga ccgatccgga
     6301 cttcttcacc cacgtccgga cgggccagct catcgtggac accggtcagg tcccaaccgc
     6361 cgatccgttc tcgttcacct ggttcgggca gccatggacc ctccacgagt ggctgagcga
     6421 ggtgctgatg tacctcctga tcagtggccc cggtgtgatg ctctcgctca tcaccttcgg
     6481 gctcatcccg ggcgctctcc tcgcggtcct gctcgtctcc gtcctcaagc gcggcgtccc
     6541 gctccgcccg gccgcactcg cctgcatcct cgccgcctgg gtgttcatcc cctacgtcac
     6601 catccggccg caggcgatca gttggttact gatggcggtg ctggtggcct tcctctgggg
     6661 cctcgatgcg gccaaacccc gacgcgcgct gtggctcatc cccctgttca tcctgtgggc
     6721 caacgtccac ggcctgtggg tggtcggcct gggcgttgtg gcgctctacg gcctcttcac
     6781 tgtcgccgga cgcaccccaa tggccggcgc cggtggccgg cagtggatcg gcctggcctt
     6841 cgtcggctgc ctgctggggg tcatgctcac cccggcgggt ccggccggcg tcctgtaccc
     6901 cctccgctac atcgatgccg gggactgggg actggccaac atccatgagt ggcagtcgcc
     6961 cgacttccat ggcgccgccc acctgggcct cctgaccctg atcctcaccc tgctcgccgt
     7021 cgggatctgg ggaggcgcgc ccggctggat gagcgtcctc accatcctgg gcgtcgccat
     7081 gagcctgatc tcgctgcgga atgccccgct ccttgccgtg tggtcagtcc cggtggtcgc
     7141 catggggctc gccgctcgat ggcccgcgcg gctcaccgcc cgcccggtcc ccgccagcca
     7201 ggagcccgtc cgtcgagcaa tggaggccat cgccgccgtt gcggtcgtcg tgatcagcgc
     7261 ggtcctcgtc ctgccccaga ccccggccgt gcacctcgag gagacggtgg cgtccgaatt
     7321 ccctgaggcg gccatggaga tcatccgcga ggtcaacccc gatgcccgtg tcctggccga
     7381 gtacggctgg ggcggctacg tcatctggtc cggttacgac cacggcgttc gcgtctttgt
     7441 cgacggccgg aacgacatgt acgaccaggc catcctcgag gactacagcg ccatccgtgc
     7501 agccgatccg ggttgggagc agctcctcgc ggaatacgag gtcgaggcca tgatctggcc
     7561 gccggaggcg gtgctgactc gcggtctgct ggacggcccc ctcgcggacg ggaccgaatg
     7621 gtgcgaggtc ttccgggacg agacccagat cctgtatctc cccgaatgcc tgccggactg
     7681 actcgtggcg ggccgtcgcc ggcccgaatc ccctggctgc tcatcggtct ggtcaccctc
     7741 gtcgggttgg cggttcgcgc cataccggtc gcgctggcgg actttccggt caatgacggc
     7801 gggctgttcc tggccatgac ccgagcgatc caggacgccg gctgggcgct gccggccacc
     7861 gtcgcctgga acgggaacgc cctgccgttc gcctacccgc cgctggcctt ctacggggcg
     7921 ggtgcgctcg aggccgtgct cgggatcgac ctcctcacgg tatttcgatg gatgcccctg
     7981 gtcgcgagca cgctgatcgt gccggccgtg ttcctcctcg gtcgcgcggt cctgcgctcg
     8041 gacctgggtg gcgtggtcgc cgccctcgcg tacgccctcg cgcccgcgtc gtatgtctgg
     8101 atgatccagg gtggcgggat cacccgatcg cccggtctgc tgctcgccgt cctcgccgta
     8161 tggcagagcg tgtttctcgt ccgcgaaccg acgcgacgtc gcaccgtggt ggtcgggctg
     8221 ctcgccggca tgaccgcgct gatccatccc gccgccgcgc tgttcgccgc gatcagcggc
     8281 gtcctgctcc tggtcttcga gggccgttcc cgcgagtcac tcgcccatgc gacggccgcg
     8341 ctgggggtgg cggccctggt cgcgctgcca tgggccgcga tcgtcatctc tcggcacggg
     8401 ttcgccgccc tgaccgatgt gcccagcaac gggcccgatc cggtcacggc gctgctcgcg
     8461 gtctttgccg gtcgcgtgac cggcattccg ttcgtcagcc cgctggccct cgtcggcgca
     8521 gccctggtgg tgctggctct gctccgccgg cagtggttcc tgccgctgtg gttcgcggcg
     8581 tcattagtgc tgtcctacca gtacgcgatg atcccattcg ggatgctcat cggcgtgctc
     8641 gtagtcgacc ttgtcgccct ccgcgccagg gatcacgagc cggccgccgc agacaaatcc
     8701 gttcgctgga ttcctgtgtt cggcgtcgtg gccctcgcga cgtgcttcgt tatcgaaggt
     8761 gttggctcag cggcgaccgt ccttaacccg agtgcacctg tccatgccct gagtgccgac
     8821 cgtcgcctag ccatggccga ggtcgccgcc atgccagatg gcacattcgc cgtgatcacc
     8881 ggcagcgaat ggtcgggcga ccccgactcg gagtggttcc cactcctcgc ggatcatcca
     8941 tccgtggcca ccgttcaggg agcggagtgg ctcggccaag aggccttcga ggatcaggtc
     9001 atcgcctacc gggcactcca ggcctgcgtg cccacggcga ccagttcatg cgtttccgaa
     9061 tggctcgatc tatggccggc ggactacctc tacctgccga acggccggct gcatggtccg
     9121 aactctccgc ccgactgctg cacggcgttg cgggaggccc tgcgaacaga ccccgcgttc
     9181 accgtcattt acgacgagtc tggcgggacg atcttcgtcg acagcgaatt gacacggagc
     9241 gaaccggccg ccgtgcgcta gggcagctcg atcacggggt gcccacgaat ggaagaatcc
     9301 gaacgatcgc cgcgcacagg agtgccaatg cgagaccaaa tccaaccgcg gccggtccaa
     9361 cgtcccggtc cgcacggttc ttcgtcacgg tcacgccgcc ggccatccgc gggaggctag
     9421 catcagcgcg gatgggttca ataccgagat cgtgggcggg aagagtcgat ggctgagcgc
     9481 cgaatcgccg tccttggcgc cggggcattg gggctgaccg cagcgcttcg tctcacccag
     9541 cggggcgacc gcgtcacgct cttcgagcgg gagccggtcc cagggggtct ggcagccggc
     9601 ttccagcccg ccgggtcaga cggcccatgg ctcgagaagt tctaccacca cttgttccag
     9661 agcgaccacc acgccatcgc cttgatcaac gagctgggcc tgggctcgga cctgagctgg
     9721 caccgacctc ggaccgtggt cctccgggac ggcgccctgc accagcttga ttcgccgatg
     9781 tcgctgctcc ggtttcgtcc gctcccggtg atggatcgga tccggatggg tctcgcgctg
     9841 gcgtacctca agacgctccg cagtccgcga cggctcgagg gggcgcgtgc ggctccatgg
     9901 attcggggac agatgggaga ccgagccgct gaggtggtct gggacccctt gctgcgagcg
     9961 aagttcggcg acgcggccga aaccatcgcc ctcccctggt tctgggcacg ggtccacgat
    10021 cggacgaccc gtctcggcta cctcggtggt gggttccagc ggctgtacaa cgagctggcg
    10081 gatcggatcg ccgccggtgg tggagtcctg cacctcggcc gcgacgtcac gcggatcgaa
    10141 cgttcgggcg atcggctctc ggtgacccac ggcgccggcg acggcgtcgc caccgaggag
    10201 ttcgacctcg tcgtgtcgac ccttcccacc cgcatcaccg cccggctaac ccccgagatg
    10261 ccggctgact ggcacgagcg ccacgaccct ggtgcggcgc tcggtgccca ttgcctgatc
    10321 ctcgccctgg accggccgct gaccgacgcg tactggatca acctcaacga tccgggattt
    10381 ccgtttctgg ccctggtgga gcacaccaac atgcgatccc cggccgagta cggcgggcag
    10441 cacctgatct atctcgggag ctatcgaccc atggacgacc cggtcctgaa gaccgatgcc
    10501 gacacgcttc tgaccgattg gctgccggcg ctgaagcggc tgaaccccga tgtcgaccgt
    10561 tcctggatca ccggcgtctg gggcttcgcc gcgccgttcg cccagccgat cgtgacggtc
    10621 gagtacgcca gccgaatccc accgttccag acaccggtcg cgggcctgtg gatcgccaac
    10681 atgttccagg tctatcccca cgaccgaggg cagaattact ccatcgacct cgccgagcgc
    10741 ctggtagccg ccctgggatg atggagtgcg gcgctcaatc ggcgagctcc accagcagcg
    10801 tgcgtccgtt ctcgcgaatc gtcgtcagtc cttcatccgc ggccagatca ctgcggaatg
    10861 cctcctcacc gagccctgcc gagacgaaga catgaccaat cccgtgttcg accatccagg
    10921 tccgtagaca ttctgccgag atcctggagc aacgttgcag ctcgtagttg aggagctggg
    10981 tctgctgcca gacctcggcg ccgagccatt ccgatccctg ggtcaacgtc ggattccgaa
    11041 tcccgctgat ggcaggaaac cactcacccg ggccctcccc ccaccaggga cgtccggtca
    11101 ggaccgcgac ctcggtgaga ccgctcgtgc gaagttcctc catgacctgt cggtctgacc
    11161 gtggaatctg gtcgaccggc gccgccggat tcagcggaac cgaaagggcg ccgaacaatg
    11221 cgacggcgaa cacggctcct gccagcgcct tcccggtacg cgggttcgcc cgggccagcg
    11281 ggatgccaac gccttcgatc gccgccgcgg ccatcaatgc caggggaacc gtcatgtaga
    11341 catcgcttcc ccgtggcagg gccgccacga tcgcgaggaa ccacacagga aggatccagc
    11401 gcccctgtcg aatctcgtgg agcgtcccga ccaggcccag caggaggaag acgtccagga
    11461 gcaggtgcgc cgcgccggtg taccgcaggc tggtcagccg gatgagcagc gcgccgagcc
    11521 caaatctggt cccacccgcg tccaccagga tgccgatccc gtgtgtcgcg acgacgttga
    11581 gccaccaggg cgccgcgaca acgagtgcga cgaggccgga cgcgaccagc cacggaacgg
    11641 ccggcagccg acgcgcttcg aagatgagaa tcacgagcac ggatagaccc gcgaaagcac
    11701 cagcctcggg gtgcgtcatc accgcgagac ccccgagtac cccggtgagc acgaggcgca
    11761 gccagcccgg cgatcggatg agatggaccg cactcgtgac ggcccccatc gcgaggacaa
    11821 gcccgagtcc acgggtcacg ccgccgccag tgatcagcca gtcatacgcg tgcgggatca
    11881 gggcgaaggc aagggcggcg atggcgggat gaccggggag ccggagggcg cgcgcgagcg
    11941 cgtagaagac ccccaccgac gcgacggcgt acaggaacgg gagaacccgg aacacgacct
    12001 ccatctggaa catcgccgtg ccgattgccg cgatcgcaac cccgagcggt ggatacaccc
    12061 atgggatgcc ggcgtcgttg aacgacgtct cggccggaat cgtgaagccg gacgcgagga
    12121 gctcggatgc catgaccgag aacatcccgc cgtcgtggag cgggaagtcg gaccccatga
    12181 acggcacggc ccggaccact ccgccaagaa tgaccacgag gaggagtggg agggccgatc
    12241 gcaaccggtt agtcaagtgt gggcgcgcac gatctcgtca gatacaggct tcaccgcagg
    12301 tgcatcggac tgccgctgat gcaaccaccg tcgtgactcg tgaatgacga ggggaatgcc
    12361 ggcgatcatt ggcaacatga ggttgcccca gtagatgtcg gggagcggaa cggtcatcgc
    12421 caggcccagg agcaggaatg acacgtcgtc gcgcttcgac agccacgacg cgagcagaag
    12481 caggaggccc gcgacgtagc cgaccaccac cgggagcccc acgcaggcca ccgaccacaa
    12541 cgcatacggg gggcacgctg cctccgcgtt gcccagggcg acaagccagt cggcgatcca
    12601 gcctgggtgg gcaatggcca ggagcaacgc catcgccacc gcgacgagca gcggcccctt
    12661 ccacacgccc ttcgtgcgga tggcccacaa cactcctgcg gcggggaagg ctttgatgag
    12721 gccacagagc agagccggga tcgccgacca tcgcggccag agccacatgc tgcccatcgc
    12781 ggcggccacc atgggcgaga cgtagccgct cttcaactct gagaagccaa cctggaatgc
    12841 ggttgccgtg attgcgacga ccgcgatggc catggccgcc gacatccggc ccacctcgcg
    12901 gcggaccatc aggagcacga taccgatcac caccacgaag ctgagcgcga cccacaggta
    12961 gaagaacggc tcgcccaggg tgaagggagc aagaaggtag gcaccactcg gtgggtagac
    13021 gaaccccagg ccgtagatgg ccgcgtccag cgggtacggc ccggcgagct gcatgtcggt
    13081 atacggcgtc aatcccgcat acacccgacg agcaccctcg gcgtacgaga ttgcgtcggc
    13141 ccactccacg tagaccaacg cgttggtgat cgagatccga agggacaacg ccccgattgc
    13201 catgagggcg atgaacccga cgatcttgat gggacggctt gcgctggcca ccgcgatcac
    13261 cccgcggagg gacgtgctca gaacgctcat cagtcaggca gggtacggcc agcggaaggg
    13321 gaatacgatc cgccaaatgc accattggcg taccagacgt gagtggccgc tcatcctcgc
    13381 gatcgccgtc gcggcctgta cgttcggtcg cccgcccgtg gatggcgcgg gcccgggatc
    13441 tgcttcgcca tcggtcgagg agtttccggt catcctcgcc gccggagaca ttggtgcctg
    13501 cgactcgaac ggcgacgaag ccaccgcggc gctcctcgag gagcacgatg gggcgatcct
    13561 tgcgctcggc gacctcgcgt acccggccgg tcgggccgat gacttcacga agtgctacgc
    13621 gccgtcctgg ggcccggacc gggaccgcac acacccggtg cccggcaatc atgagtacgc
    13681 gacccttcgc gccaagccgt actacgacta cttcggggcc gccgccggcg acccccagga
    13741 ggggtggtat gcgttcgacc tggccgagtg gcatgtcatt gccctgaact cgaactgctg
    13801 ggaaatcgga ggttgcagcg cagattcccg gcaggcggag tggttgcgtg ccgatctcgc
    13861 cgaccactcc tcagcctgca cgctcgcctt ttggcaccac ccgcgctgga gctcaggcga
    13921 ggtgcacgga tccgacgggg cgacgaccac gttctgggaa gtgctccacg atgcgggcgg
    13981 cgacatcgtc ctcgccggcc acgatcacct gtatgaacgg ttcgagccca tggaccggga
    14041 cggcgtcgcc gaacccgatg ggatggtgga gttcgtggtc gggaccggcg gaaagtccct
    14101 ctttgggttc gcggacatcc ttccgaccag cgccgcccat gacaactcga gctttggcgt
    14161 cctgaagctc acgctccgcc ccggtgccta cgactgggag ttcctctcca cctcgggtac
    14221 cggtttcacc gacgcgggga cgaccgcctg ccgctgagcc caccaaaacg ccgccgaacc
    14281 agccagcagg gctagcgacg aggtccagac gttgggcagg gcgagcatga ccccgatcgg
    14341 cagcagccag gcccggtccg tccgggcggc caccacgatc accaccaccg cgatcggcaa
    14401 ccggacgagg aacggcaggg gaatgacctg gatccgcgga tcggatccga cgttggccgc
    14461 gagcagcgcg aaccattcgg cccacagctg gggcacggtc aggaccgata ggcccacgat
    14521 cgccccggtg acgccgatcc cgaccgcgaa cgcgcgccac tccccgcgca acgggtgcca
    14581 caggatcccg atcccgggcg tgaccttggt gatgaacggg aacgcccacg cgcccgcgcc
    14641 ccacggttga cgccacacgg ccaagaccac ggcgccggcc aggaagacgt ggatgttgcc
    14701 gcgcaggatg tcgtcgatga cgccgggaaa ggcgagcatc cacggcacgc gcatccagaa
    14761 caggatcccg atcgcgaggc cggtccacag cgccgcgaac acgggccacg gcaggagggt
    14821 gaacgggtag atcagctggg cgaaggcggg ggagtagacg tacgcctcgt actccagcca
    14881 cgggatgtcg tacaggccac ctgaccaggc gttccagatg ccataggcgt cgaacatcgg
    14941 tccgtagtcg ggcaccggct cggcatacgg cacgatccgg agccagaccg cagcgatgta
    15001 gaccagccct gcaatcgtca ggccgtggcg cagtgcggcc caccggtcaa agcgcgcgac
    15061 attcccgttc gacgcggacg tcaccccagg gcctcgctag cgccggttct gggcaatctc
    15121 ccagatgtgg ccgccggggt cgcagaagct ggcggttcgg atgccccacg gtcgatccat
    15181 tgggccattc agcagctcga ccccccgctc gtggagggtc gtgcacatgg catccacgtc
    15241 atcgacctcg atggtgaact gcatgcgcga tccggcgtcg gctgaagcga caaccgccgg
    15301 ctcgatcaac gaggtcgccg cgctgaccct gagcaggttg atgaccgtgt tcccgaaatt
    15361 gaacacggcc gagtcggcgt cctcgaacat gaccggcagg ccgaagacct tgccgtagaa
    15421 cgtccgtgcc gcatcaaggt cctcgacgaa aagggtgatc gcgtcaatcg ccttcggcca
    15481 tgccgccgcc tccgggtctg ccatcgctac ctccgaatca cgggaacagg tcgggattgg
    15541 ggtcgatctc gatccgaggc tggctcatcg ggccgccgag cgggtagtcg aggattggca
    15601 gctgcatgcc ggacaacttg aaggcgccga ccagcagaac gaccaccagc acaaccgccg
    15661 cgaggaatcg catggggccc agcctactcc ggcctcccca gcccccgaca ggttcctttg
    15721 ggctaccccg ggtgtcctgc cccgatccgg cctac
//
LOCUS       DASYGU010000269         6348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1330393, whole genome shotgun sequence.
ACCESSION   DASYGU010000269 DASYGU010000000
VERSION     DASYGU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330393"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGO32_07780"
     CDS             complement(<1..239)
                     /locus_tag="VGO32_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV7200686.1"
                     /translation="MISIQAIRDDPDAVKRAIARKGESSEVIDRLLEADSQRRELLAA
                     ADAAKHERNEGSRQVGEYVRAGQTAEAEARKASLAS"
     gene            complement(253..1887)
                     /gene="lysS"
                     /locus_tag="VGO32_07785"
     CDS             complement(253..1887)
                     /gene="lysS"
                     /locus_tag="VGO32_07785"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV7200687.1"
                     /translation="MFWADDIAARAAAAGQASGSGAPIVVNDSKTPSGTVHVGSLRGP
                     VIHDEIWRALRRAGHDAVYRYGVDDLDPMDSQALLTPDAVDRYMGVPLAHVPAPAGSG
                     AASYARHFVGELFLGTFEPLGLHPEVYWMSDVYAAGDMDRFIRAALDGAETIRTIYRD
                     VSHVDKEPGWLPLSVICTTCGRVGTTIATDWDGDTVAFECRPDLVTWATGCGTVGRVS
                     PFGGTAKLPFNVDWAVKWSLFDIALEGCGKDLATAGGSRDRSDAISRQVFDREPPINV
                     PYEFINVGGKRMSTSRGTGVPAHEITAVMPPDELRLLFVRHRPNVAFEFDPIQTDAVP
                     RQVDEFDRLAAATAGRPVRGELPADADRIFAASLVNAEADVSAEAGVFRPPFAHLALL
                     VQLPGVDVEERVAAENGSPLNARERTILAERTRAVRAWLDTYAPDAARIEVQHDGVPE
                     AVSALTDDQRGFLAALMDVVADLPKEQWTGEALQAAVFATAKEREIGAGAGFAALYAA
                     FLGRSSGPRAGWLLASLDRSFVVERLEAAGQSGTSA"
     gene            complement(1898..2359)
                     /gene="greA"
                     /locus_tag="VGO32_07790"
     CDS             complement(1898..2359)
                     /gene="greA"
                     /locus_tag="VGO32_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV7200688.1"
                     /translation="MNNKPVYLTQEGVERLEAELRHLITEERPRVAARIHDAKLDGDI
                     SENAEYEDAKQEQSFLEGRIATIEVQLKNHEVIEHQNGDKVRLGSTVVIQGPDGKETL
                     TIVGSAEAAPREGRISNESPVGVALMGHKKGEEVTVQAPAGPVTYTLVSIR"
     gene            complement(2526..3371)
                     /locus_tag="VGO32_07795"
     CDS             complement(2526..3371)
                     /locus_tag="VGO32_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV7200689.1"
                     /translation="MDVIFQAFVMGLVQGLTEFIPVSSSAHLELVPWILGWEQDALIG
                     SLSFDVFLHLGTLIALLVNVWRDWLRLMRAMVRSIIERRIGEDRDRRLGWLLVLASIP
                     AALIGFAFEDAIDAAFHGDDDMSRLAICGFLVVGAIVLFIADRYGSRERDLDHVGAGR
                     ALTIGLSQAAALLPGISRSGATMSTALAFGLTRETAARFSFLLATPITLGAGLYGSRH
                     LLTASHTSTEWVAVVVGFAAAAVSGALAIGFLLRWLRTRSMAVFSLERIVLAALVLVL
                     LAVGH"
     gene            complement(3410..5614)
                     /locus_tag="VGO32_07800"
     CDS             complement(3410..5614)
                     /locus_tag="VGO32_07800"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEV7200690.1"
                     /translation="MEIQPITWVIPVAGLTAVLFAIYLARDVLSRDTGTTAMEDVAGT
                     ILEGAVAFIRRQYLTILVLAVVGAVIIGVVIGLLETPEVADTDVFGIQLGAMTAVAFL
                     VGALCSMASGVIGMYISVKSNVRTAAAAQHSLVGAVQVAMRGGAVSGFLVVALSLLGV
                     WGIFVAYGGFTNPAVAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGSDLVGKVEAG
                     IPEDDPRNAGVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGVYVIAQSAGWP
                     NPESWIFFPLVIRAFGLFATIVAIFFVRGKETEDPMRMLNRGYWVTTALSVVALAFVT
                     NVMMNTGGEPGAYGIPVWVWFFFAGVVGLATSVAFVYITQYYTAGAWRPVKEIAEASK
                     TGPATNIIAGTAVGFETTLVTALTIGIALFASHWLGSQANLVNAAGADVGGIFGTAVA
                     TMGMLMTTAYILAMDTFGPITDNAGGIAEFAKAEGGAREITDRLDAVGNTTKALTKGY
                     AIASASLAAFLLFSAYIDKVNLIFTRQGRPPMESVNLAEVSVFIAALIGAMLVFFFSS
                     LAIRAVGTTAQSIIVEVRRQFREMPGIMDYTQRPDYARVVDITTAAALRQMILPGMVA
                     VATPILVGLLLGHEAVAGLLMVGTISGVMLATVLNNGGGAWDNAKKYIESGHLLDDDG
                     NVLGKKTFAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLFIRG"
     gene            5810..>6348
                     /gene="lepB"
                     /locus_tag="VGO32_07805"
     CDS             5810..>6348
                     /gene="lepB"
                     /locus_tag="VGO32_07805"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV7200691.1"
                     /translation="MQPAAEPPADDRSAARRGAGCLLEIVETIVLTLVIYLLIHNFVA
                     QPFEVEQNSMVSTIQPSDYVLIDKLTPHWNDYVRGDIVVFQPPNGYEQGGVPFIKRVI
                     GLPGETVRLENGAVFVTSINGVEVRLTEGYVVTGTDGRPSPTLPRDAEGTDRWVIPEG
                     MYFVMGDNRPESQDSRFFGP"
BASE COUNT         1036 a         2125 c         2229 g          958 t
ORIGIN      
        1 gaggcgaggg atgccttgcg cgcctcggcc tcggcagtct ggccggcgcg gacgtactcc
       61 cccacctggc ggctgccctc gttgcgctcg tgcttggccg catcggccgc ggcgaggagc
      121 tcacgccgct ggctgtcggc ctccagcagg cggtcgatga cctcggaact ctcgcccttg
      181 cgcgcaatcg cgcgtttgac cgcgtccgga tcgtcgcgaa tggcctggat cgagatcacg
      241 ccacgcccct tgtcacgcgg aagttccgga ctgtccggcg gcttcgagcc gctcgacgac
      301 gaacgaccga tccagcgagg cgagaagcca gccagctcgg ggaccggacg accgtcctag
      361 gaaggcagcg tacagggcgg cgaagccggc ccccgccccg atctcgcgtt ccttcgcggt
      421 cgcgaacacg gcggcctgaa gtgcctcccc tgtccattgc tccttcggca ggtcagcgac
      481 cacatccatc agtgcggcga ggaatccgcg ctggtcatcg gtcagggcgc tgaccgcttc
      541 tggaacgcca tcgtgctgga cctcgatccg ggcggcgtcc ggggcgtacg tgtccagcca
      601 ggcgcggacg gctcgggtgc gctcggccag gatcgtgcgc tctcgcgcgt tcagcgggga
      661 gccgttctcg gcggcgaccc gctcctctac gtcgacgccg ggcagctgga cgaggagcgc
      721 gagatgggcg aacggcggcc ggaatacgcc ggcttcggcc gagacgtccg cctcggcgtt
      781 aacgagcgag gcggcgaaga tgcggtccgc gtcggccggc agctcgccac ggaccgggcg
      841 gccggcggtc gccgcggcca gccggtcgaa ctcgtccacc tggcgcggga cggcatcggt
      901 ctggatcggg tcgaactcga atgcgacgtt gggccggtgg cggacgaaga gcagccgcag
      961 ctcgtccggc ggcatgaccg cggtgatctc gtgtgccggg accccggtcc cccgcgaggt
     1021 gctcatccgc ttgccgccga cgttgatgaa ctcgtacggg acgttgatcg ggggctcgcg
     1081 gtcgaagacc tggcgcgaga tggcgtccga gcggtcgcgg gaaccgccgg cggtggccag
     1141 gtccttgccg cagccctcga gggcgatgtc gaacagcgac cacttcaccg cccagtccac
     1201 gttgaacggc agcttggctg tgcccccgaa cggcgaaacc cgcccgaccg tcccacagcc
     1261 ggtcgcccag gtgaccaggt cggggcggca ttcaaaggcc accgtgtcgc catcccagtc
     1321 ggtggcaatc gtggtgccca ccctgccgca ggtcgtgcag atgaccgaca gcgggagcca
     1381 gccgggctcc ttgtccacgt ggctcacgtc gcggtagatg gtccggatag tctcggctcc
     1441 gtcgagggcc gcgcggatga atcggtccat gtcgcccgcg gcatagacgt cgctcatcca
     1501 gtacacctcc gggtggaggc caagcggctc gaaggtgccg aggaacagct cccccacgaa
     1561 atggcgggca tagctcgcgg cgcccgaccc ggccggggcc gggacatggg ccagtggcac
     1621 gcccatgtat cggtccaccg cgtcgggggt cagcagcgcc tgggaatcca tcgggtccag
     1681 gtcgtcgacc ccgtagcgat acaccgcgtc gtgcccggcg cgacggagcg cccgccagat
     1741 ctcgtcgtgg atgaccggac cgcgcaggct gccgacatgg accgtgccgg aaggcgtctt
     1801 ggagtcgttg acgacgatgg gcgcccctga accggacgcc tggcctgccg cagccgccct
     1861 ggccgcgatg tcgtcggccc agaacatgcg gcgaacgcta gcggatgctg accagcgtgt
     1921 acgtcaccgg accggcaggt gcctggacgg tgacctcttc ccccttcttg tggcccatga
     1981 gcgccacgcc gacgggcgac tcgttgctga tccgaccctc gcgcggggcg gcctcggccg
     2041 agcccacgat cgtgagcgtc tccttgccat cgggtccctg gatgaccacc gtggagccga
     2101 gccgaacctt gtcgccgttc tggtgctcga tgacctcatg gttcttcagc tgcacctcga
     2161 tggtggcgat cctcccctcg aggaacgact gctcctgctt cgcgtcctcg tactccgcgt
     2221 tctcgctgat gtcgccgtcg agcttcgcgt cgtggatccg cgcggcaacc cgcggccgct
     2281 cctcggtgat gaggtgacgg agctccgcct cgaggcgttc gacgccctcc tgggtcaggt
     2341 agaccggctt gttgttcatg tcgtcggggg tcactcgtac ggttgggctg tcagaacgcg
     2401 gccctgataa caaaaaagcc ccagttcccg cggttgtcgg gagactggct ctcgggtgct
     2461 ggcgtcggcg gattgtaggc gcggggtgca ccggtgtcaa aggaaccggc ccgtctcccg
     2521 gaccctcagt gaccgacggc caggaggacc aggacgaggg cggccagcac gatccgctcc
     2581 aggctgaaca cggccatgga ccgggtccgc aaccagcgaa ggaggaagcc gatggccagc
     2641 gcgccggaca cggcagcggc cgcgaacccg accacgaccg ccacccactc ggtcgaggtg
     2701 tggctcgcgg tcagcagatg ccgggagccg tagagtcccg cgcccagggt gatcggcgtg
     2761 gcgagcagga agctgaagcg agcggcggtc tcgcgggtga ggccgaacgc gagggcggtg
     2821 gacatggtcg cgcccgatcg gctgatgccg ggcagcaggg cggccgcctg ggacaggcca
     2881 atggtgagcg cccggccggc tccgacatgg tcgaggtccc gctcgcgcga tccgtaccgg
     2941 tcggcgatga acaggacgat ggcgccgacc accaggaatc cgcagatggc caggcgcgac
     3001 atgtcgtcgt caccgtggaa tgcggcgtcg atggcgtcct cgaatgcgaa cccgatgagc
     3061 gccgcgggga tggaagcaag gaccaacagc cacccgaggc gtcgatcgcg gtcctcgcca
     3121 atccgccgct cgatgatgga ccgaaccatg gcccgcatca ggcgcagcca gtcgcgccag
     3181 acgttgacca ggagcgcgat cagggttccg aggtggagga atacgtcgaa tgacaggctg
     3241 ccgatgagcg cgtcctgctc ccagcccagg atccacggca cgagctccag gtgggccgag
     3301 gagctgaccg ggatgaactc ggtcaggccc tggaccaggc ccatgacgaa cgcctggaag
     3361 atcacgtcca ccggctcagc ctcggttcag gcgtccgggc gtttcgcgct cagccgcgga
     3421 tgaacagtgg ggcgaggacg agggtgatgg tcgcgagcag cttgacgagg acgtggagtg
     3481 acgggccggc cgtgtccttg aacgggtcgc cgaccgtgtc gccgacgacc gctgcggcgt
     3541 gggcgaacgt cttcttgccg agcacgttgc cgtcgtcatc gagcagatgc ccggactcga
     3601 tgtacttctt tgcgttgtcc cacgccccgc cgccattgtt caggacggtg gccagcatga
     3661 cgccggagat ggtgcccacc atcagcaggc cggcgacggc ctcgtggccg aggagcagtc
     3721 cgacgaggat gggggtcgcc accgcgacca tgccgggcag gatcatctgg cgcagcgcgg
     3781 ccgcggtcgt gatgtccacc acccgcgcgt agtccggtcg ctgggtgtag tccatgatcc
     3841 cgggcatctc ccggaactga cggcggacct cgacgatgat cgactgggcc gtcgtcccca
     3901 ccgcccggat ggccagcgag ctgaagaaga agacgagcat cgcgccgatg agcgcggcga
     3961 tgaacacgct gacctccgcc aggttgactg actccatcgg cggccggccc tggcgggtga
     4021 agatgaggtt gaccttgtcg atataggcgc tgaacaggag gaaggcggcc aggctcgcgc
     4081 tggcgattgc gtagcccttg gtcagggcct tggtcgtgtt cccgaccgcg tcaaggcgat
     4141 cggtgatctc gcgggcgcca ccctcggcct tcgcgaactc cgcgatcccg ccggcgttgt
     4201 cggtgatcgg tccgaaggtg tccatggcca ggatgtaggc ggtggtcatg agcatgccca
     4261 tggttgcgac cgcggtcccg aagatgccgc ccacgtcggc accggcggcg ttgacgaggt
     4321 tggcctgcga tccgagccag tggctggcga acagcgcgat gccgatcgtg agcgcggtga
     4381 ccagcgtggt ctcgaacccg acggccgtgc cggcgatgat gttcgtggcc ggaccggtct
     4441 tgctcgcctc ggcgatctcc ttgaccggtc gccatgcgcc ggcggtgtag tactgggtga
     4501 tatagacgaa tgcgacgctg gtggcgaggc cgaccacgcc cgcgaagaag aaccagaccc
     4561 agacgggaat gccgtacgcg cccggctcgc cgccggtgtt catcatcacg ttggtgacga
     4621 acgcgagggc gacgaccgac agcgccgtcg tcacccagta gccgcggttc agcatccgca
     4681 tcgggtcctc ggtctccttc ccgcggacga agaagatggc gacgatcgtc gcgaacaggc
     4741 cgaaggcacg gatcacgagc gggaagaaga tccacgactc ggggttgggc cagccggccg
     4801 actgggcgat gacgtacacg ccgacgccca ggatcatggc cccgatgttc tcggccgcgg
     4861 tggactcgaa caggtcggcc ccacgaccgg cgcagtcgcc gacgttgtcc cccaccaggt
     4921 ccgcgatgac ccccgcgttg cgcgggtcgt cctcggggat gccggcctcg accttgccca
     4981 cgaggtcgga gcccacgtcc gcggccttgg tatagatccc gcccccgagc tgggcgaaca
     5041 gggccacgaa gctggccccg aacccgaagc ccacgatcag gaatggggcg acggccgggt
     5101 tcgtgaaccc gccataggcg acgaagatgc cccacacccc gaggagggag agcgcgacga
     5161 ccaggaaccc ggaaacggct cctccgcgca tcgccacctg gaccgccccg acgaggctgt
     5221 gctgggcggc cgccgcggtc cgcacgtttg acttgaccga gatgtacatg ccgatgacgc
     5281 ctgacgccat cgagcacagg gccccgacca ggaaggccac ggccgtcatt gcgcccagct
     5341 ggatgccgaa cacatcggtg tcggcgactt cgggggtttc gagcaggcca atgacgacgc
     5401 caatgatcac cgcgccaacc acggccagga cgaggatggt caggtactgg cggcggatga
     5461 aggccacggc tccttcgagg atggtgccgg ccacgtcctc catggcggtc gtcccggtgt
     5521 cgcgactcag gacgtcgcgg gcgaggtaga tggcgaacag cacggcggtc aggccggcga
     5581 cggggatcac ccaggtgatg ggctgaatct ccatggatcc tccgagcggg ggttgggcga
     5641 cggcgccgag ggcgcggtac atcaggggga acacacagga aacgccggcg aggtaccgat
     5701 ttcgggtccc gccagcgagg cggcgccatg gtagcgcgca gccgcgagaa gggtcaaccg
     5761 cacgggccga tcccgattcc ggggtatgct tccggccgtt ttgacctacg tgcagccagc
     5821 cgcagaacct cctgccgacg accgttctgc ggcgcgtcgg ggagcgggct gcctgctcga
     5881 gatcgtcgag acgatcgtcc tcaccctcgt catctatctg ctcatccaca acttcgttgc
     5941 gcagccgttc gaggtcgagc agaactcgat ggtgtcgacc atccagccca gtgactacgt
     6001 gctcatcgac aagctcacgc cgcactggaa cgactacgtc cgcggcgaca ttgtcgtctt
     6061 ccagccgccg aacgggtacg agcagggcgg agttccgttc atcaagcggg tcatcggcct
     6121 gccgggcgag accgtccgcc tcgagaacgg cgccgtgttc gtgacctcca tcaacggggt
     6181 cgaggtccgc ctgacggagg gctacgtcgt aaccgggacc gatgggcggc cgtcgccgac
     6241 tctgccacgc gacgccgagg gtacggatcg ctgggtgatc cccgaaggca tgtacttcgt
     6301 catgggcgat aaccggccgg agagccagga ctcgcgcttc ttcgggcc
//
LOCUS       DASYGU010000270         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1742048, whole genome shotgun sequence.
ACCESSION   DASYGU010000270 DASYGU010000000
VERSION     DASYGU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742048"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGO32_07810"
     CDS             <1..724
                     /locus_tag="VGO32_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV7200692.1"
                     /translation="QELFYGPYFCQVQDPQYFSYTEHIPDGPTTKRMQELAKETGMVL
                     IVPMYEEDDQTTGIYYNTAAVIDADGSYLGKYRKTHIPHVKGFWEKYYFRPGNMGYPV
                     FDTAVGKVGVYICYDRHFPEGARALGLNGAEIVFIPSATSRGLSEYLWRVEQVSHALA
                     NGYFVGTINRVGIEPLGDDDFYGQSYFVDPRGQFIGDVGDAHDEELIVRDLDLDKIEE
                     VRQTWQFFRDRRPDAYDDLVRP"
     gene            747..1769
                     /locus_tag="VGO32_07815"
     CDS             747..1769
                     /locus_tag="VGO32_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV7200693.1"
                     /translation="MDFGFTLKLENETGRTLDLARRAEAAGFAYGWVFDSHVLWRDPY
                     PALTLMAGVTERMRLGTCVTNPATREPSVTASTLAELNEISSGRMDLGIGRGDSARRV
                     LGKPPTTMKTLEEAISVIRDLVEGRPVTYEGTELQLTWTGKWRLPVWVAGYGPMALAM
                     TGRVADGLILQLADPDLIRWFVAQLREAEAAAGRTPGEIKVQAAAPAHVGSRDVGQDR
                     TRWFPALVSNHVVDLVNKYPREQLPEALTGYITDREGYDYHHHAEVGSTNAAFVGDEV
                     TDRFCILGEPAEHVAKLLALADAGVDQFNLYLMNGDEEEQLDRYGREVVPALRAVASN
                     RQGAST"
     gene            1766..2287
                     /locus_tag="VGO32_07820"
     CDS             1766..2287
                     /locus_tag="VGO32_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200694.1"
                     /translation="MKVSFVAGYGPITPDSAASLKFWRDDLGLPMTEIAPDYWGTDDV
                     PGVNAFAAWPLGEAAESCFGSKTWPEHITAPQAWLELDVETPEAVSVAAADLEAAGNE
                     LLKPPTTEPWGQTVARLLSPEGLLVGITYTPWMHPHEHGDHDGHDHGEHDHDPGEHDH
                     DHEGHDHGEHANH"
     gene            2297..2767
                     /locus_tag="VGO32_07825"
     CDS             2297..2767
                     /locus_tag="VGO32_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7200695.1"
                     /translation="MSWTDDRLLILGPEEGDAHPGDVIQKVEAAQTGGHWGVAVVSGV
                     PGEGGQTHIHVGEAEAFFIIEGEVEFLGAESRTPLTVGAFVLVPPDTEHGIRITGTGP
                     ARWLAIWPAALDGMFSEIGSIEDVNQEAYDQVRLRHGVRAGRDRRAEFEGPLSD"
     gene            2831..>3137
                     /locus_tag="VGO32_07830"
     CDS             2831..>3137
                     /locus_tag="VGO32_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV7200696.1"
                     /translation="MARKPSTDELSPFATPALVVLAVLADGPKHGYAIMQESERLSGQ
                     AMGPGTLYAALARLERRGLIEAVASDDRRRPYRLTGLGATTLQAHLDQLQSFAHRALG
                     "
BASE COUNT          539 a         1080 c         1050 g          468 t
ORIGIN      
        1 ccaggagctg ttctacgggc cgtacttctg ccaggtccag gatccgcagt acttcagcta
       61 caccgagcac attcctgacg gtccgaccac gaaacggatg caggagctgg cgaaagagac
      121 cgggatggtc ctcatcgtgc cgatgtacga ggaagacgac cagacgaccg gcatctacta
      181 caacacggcg gccgtcatcg acgccgacgg gagctacctg ggcaagtacc gcaagacgca
      241 catcccccac gtgaaagggt tctgggagaa gtactacttc cgcccgggga acatgggata
      301 cccggtcttt gataccgcgg ttggcaaggt cggcgtgtac atctgctacg accgccactt
      361 ccccgaaggc gcacgggcac tgggcctgaa tggcgccgag atcgtcttca tcccgtcagc
      421 cacgtcgcgc ggtctgtccg agtacctctg gcgcgtggag caggtctcgc acgccctcgc
      481 gaacggctat ttcgtgggca cgatcaaccg ggtcggtatc gagccgctcg gcgacgacga
      541 cttctacggc cagtcctact tcgtcgaccc gcgcgggcag ttcatcggcg acgtgggtga
      601 tgcgcacgac gaggagctga tcgtgcgcga cctcgacctc gacaagatcg aggaggtgcg
      661 ccagacctgg cagttcttcc gggatcgccg accggacgcc tacgacgacc tcgtccggcc
      721 gtaggtccgg cgcggcagcc tgaccgatgg acttcgggtt caccctcaag ctcgagaacg
      781 agaccggccg aacgctcgac ctggcgcgcc gcgccgaggc ggccgggttc gcctatggct
      841 gggtgttcga cagccatgtc ctgtggcgcg atccgtatcc cgcgctgacg ctgatggcgg
      901 gggtcaccga gcggatgcga ctcggcacct gcgtcacgaa tccggcaacc cgcgaaccgt
      961 cggtgacggc gtccaccctg gccgagctga acgagatcag cagcggccgg atggacctgg
     1021 gcatcggtcg gggcgacagc gccaggcggg tcctgggcaa gccgccgacc acgatgaaga
     1081 cgctcgagga ggccatctcc gtcatccgtg acctggtcga ggggcggccg gtgacctacg
     1141 agggcaccga gctgcagctg acctggaccg gcaagtggcg gctacccgtg tgggtcgccg
     1201 gctacggacc gatggcgctg gccatgaccg gccgtgtcgc cgatgggctc atcctccagc
     1261 tcgcggaccc tgacctcatt cgctggttcg tggcccagct ccgcgaagcc gaggccgccg
     1321 ccgggcggac cccgggcgag atcaaggtcc aggccgctgc ccccgcgcac gtgggctcgc
     1381 gtgacgtggg ccaggaccgc acccgctggt ttccggccct cgtcagcaac cacgtcgtcg
     1441 acctcgtgaa caagtacccg cgcgagcagc tgcccgaggc gctgaccggc tacatcaccg
     1501 accgcgaggg gtacgactac caccaccacg cggaggtggg ctcgaccaat gccgcgttcg
     1561 tgggcgacga ggtgaccgac cgcttctgca tcctcggcga gccggcggag cacgtggcca
     1621 agctcctcgc gctcgccgat gccggcgtgg accagttcaa cctgtacctc atgaatggcg
     1681 acgaggaaga gcagctggac cgatacggcc gcgaggtcgt gcccgctctt cgcgcggtcg
     1741 cctccaaccg ccagggagcc tcgacatgaa agtctcgttc gtggccggct acggaccgat
     1801 cactcccgat tcggccgcca gcctgaagtt ctggcgtgac gacctcggac tgcccatgac
     1861 tgagatcgcg cccgattact gggggaccga tgacgtcccc ggcgtgaacg cgttcgccgc
     1921 gtggccgctc ggcgaggctg ccgagtcctg cttcggctcg aagacctggc cggagcacat
     1981 caccgcgccg caggcctggc tcgagctgga cgtcgagacg cccgaggcgg tgagcgtggc
     2041 tgccgccgat ctggaagcgg cgggcaacga gctcctgaag ccgcccacca ccgagccgtg
     2101 gggccagacg gtcgcccgtc tcctcagccc ggagggcctg ctcgtgggca tcacgtacac
     2161 gccctggatg cacccccacg agcacggtga ccacgacggt cacgatcatg gcgagcatga
     2221 ccacgacccc ggcgagcatg accacgacca cgagggccac gaccacggcg agcatgccaa
     2281 ccactgatca gcgtcagtga gctggaccga cgaccggctg ctgatcctag gcccggagga
     2341 gggcgacgcc cacccgggcg atgtcatcca gaaggtggag gccgcgcaga cgggcggcca
     2401 ctggggcgtt gcggtggtct ccggcgttcc gggcgagggc ggacagacgc acatccatgt
     2461 cggcgaggca gaggccttct tcatcatcga gggcgaggtc gaattcctcg gagccgaaag
     2521 ccggacgccg ttgaccgtcg gcgccttcgt cctcgtcccg cccgacaccg agcacggaat
     2581 ccggatcacg ggtaccgggc cggcccgttg gctcgcgatc tggccggcgg ccctggacgg
     2641 gatgttctcg gagatcggat ccatcgaaga cgtgaaccag gaagcctacg accaggttcg
     2701 tctgcgccac ggcgtgcgcg ccggccgcga ccggagggcc gaattcgagg gtccgctcag
     2761 cgattgacat atatcgtgtc acgatatatc gtgatgcgac atacgttccg cataaccgag
     2821 agggtcagcc gtggccagga agccatctac cgacgagctc agcccattcg cgacgcccgc
     2881 gctcgtcgtc ctcgccgtcc tcgccgatgg gccgaagcac ggctacgcga tcatgcagga
     2941 gtcggagcgc ctcagcggcc aggccatggg gccgggcacg ctctacgccg ctctcgctcg
     3001 gcttgagcgt cgcgggctca tcgaggccgt cgcgtcggat gaccgtcgcc gcccgtatcg
     3061 gctgacgggc ctcggggcaa cgacactcca ggcccacctc gaccagctgc agtcgtttgc
     3121 ccatcgggcc ctcggac
//
LOCUS       DASYGU010000271         6454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1279518, whole genome shotgun sequence.
ACCESSION   DASYGU010000271 DASYGU010000000
VERSION     DASYGU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279518"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VGO32_07835"
     CDS             <1..491
                     /locus_tag="VGO32_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7200697.1"
                     /translation="RWVTPVEAIEPKALFDYLRSRRGAGSASGELVAAPGAARRVLEG
                     LRHHEMVAIAADRDVGTATVEVEFFGHPARMPSGPATLALLTGSPVVIITLRRTGHGQ
                     FVAWSEQIPWTATGDRPADIQALTQRITDTLARHISEAPEQWWGSFQPVWDDLTPGTV
                     QA"
     gene            491..1237
                     /locus_tag="VGO32_07840"
     CDS             491..1237
                     /locus_tag="VGO32_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEV7200698.1"
                     /translation="MDRSPSAGDRGKADLHLHTLYSDGTARLVDLLDWIEHRTDLDLV
                     AITDHDRIDGATRAAAMHAAGDYRFQFVIGEEITTRSGHVVGLFLEERIPPFRSVAET
                     IERIHAQGGLAVAAHPLGVFLSLGSGSLLRLQSDPNPQRHLDAVELINPSLGGRLRHG
                     ALARLNAERLHLPGMGNSDAHVLETVASAWTWFPGSSADDFRAALDAGLVEPGGSHWS
                     TGHNVAVYGRQLMAKARKLGHTLRPSGEWR"
     gene            1241..2431
                     /locus_tag="VGO32_07845"
     CDS             1241..2431
                     /locus_tag="VGO32_07845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7200699.1"
                     /translation="MIEAPEATGRDARVEPSSPPAPRAPLKIGIVSPYGFPHPGGVNE
                     HVRHTYEELRSLGHDAWIITPKYGAERDSEGRVIRLGTGWAVPSNGSVGRLTLGWRLR
                     PRIRDLLAAHRFDVLHYHEPFVPFLGPAVLSESETVNIATFHAFGGFSPSYWVGSRGT
                     TRLTARLHGRIAVSGAARHFIGRYFPGDYRIIPNGVEIEPFASAQPFEELRDGTLNIL
                     FVGRLEDRKGLTYLLRGYHRLRKRHVDARLIVVGDGPRLREYRRYVATRRIRDVEFVG
                     HVPLDAKLRYFASADIFCAPSTGQESFGIVLLEAMAAGLPIVASDIHGYKQVVSRDIE
                     GYLVEPRNPRAIAAALYKLARDPELRDRMGEAGRAKAPEYSWERVTEQVVAYYHEVRD
                     RVLA"
     gene            complement(2428..3471)
                     /locus_tag="VGO32_07850"
     CDS             complement(2428..3471)
                     /locus_tag="VGO32_07850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200700.1"
                     /translation="MTIAPVRVNVADAPPIAGLAFRRMRLPDDLEAIAGLFNTSDEAD
                     EIEDRHDVAGLAQWFAHASTWDPLEDVLLAEVDGALIGYGKVHWTEDTDGGRNYALWG
                     SVHPAWRRRGLGRAILHANQRRLREIEAGQDVRAGTDQRFETWCNETQVGAMALLASE
                     GFTTARYFFEMLRPNLDDIEEFSLPSGIEVRPVRPEDHRTIWNADTEAFADHWGGMDL
                     SEQAFRRFFSGPDFEPELWRVAWAGDEVAGQVNNMVMREFNQQTGGRRGLLAGVSVRR
                     PWRRQGLARALVSQSLVAFRERGMTDAVLGVDADNPTGALGVYEANGFVVHQRERAYR
                     RPFHEAEAPQETV"
     gene            complement(3468..4454)
                     /locus_tag="VGO32_07855"
     CDS             complement(3468..4454)
                     /locus_tag="VGO32_07855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200701.1"
                     /translation="MTDTGLRIELRPFAGEADIPELTRVANAAFAADQFQERMTEQQI
                     GLELRPDEKFDPATEMRVAEVDGTVVGFCKVEWIDTNDGLREYRSWCEVDPTWRRRRI
                     GTTLFAFARERIDELAAGQDVDRPRVAGSWASEKDLGAQALYSEHGYAPVRWFFHMNR
                     DLSQPIEVPPLPDGIEVRPVSRDDAHRLFIADNEAFLDHWGGIDSSDAAFQKWIADDD
                     FDPSLHVVAFDGDEIAGASINAIYHEANAKLGVKHGWLDSVFTRRPWRRRGLARALVA
                     RSLVVLRDQGMAEGVLGVDADNETGALGVYTDNGFVVTERFTAYRRPFEVNR"
     gene            complement(4466..5266)
                     /locus_tag="VGO32_07860"
     CDS             complement(4466..5266)
                     /locus_tag="VGO32_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200702.1"
                     /translation="MAMLTRDLRASMAFVERNFNLTKRYWGWEVAWLVYSVAGALAVT
                     LIGESLGDEHLLLVLVIGTIFWSYLAIVFEFIAEAVQWERWEGTLEYTFMAPIRRYAQ
                     LLGAAFYAVIFGLVHTLFVLLALALFFELDLSQANWATVIAFTLLGTISFIGIGIAAS
                     ILPLMYVERGAQMVFVLQSVLLLVSGVYYSIEVLPEWMQFISQFSPATYVLDGVRAGL
                     IDGVGIDQLIHDVWPLLIMAVVFIPLGLWAFGRAERYAKRTGRLKRVG"
     gene            complement(5271..6191)
                     /locus_tag="VGO32_07865"
     CDS             complement(5271..6191)
                     /locus_tag="VGO32_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200703.1"
                     /translation="MQTGLDTQAKTMTDERLAVTAEEPRVAAPARSEATPTALPALLA
                     EHVTKRFQVGRKKRPVMAINDVSLRIERGEIYGVLGANGSGKSTLIRLFSTLLTLDTG
                     KVEVFGKDIERDEMAVKRMINRVSVDAAFFKKLSPFENLAYAARLYGMDAKEAKAEAI
                     RILSRLGIAEKRLGRPVEQLSRGMQQKVAIARALLTSPILLLMDEPTTGLDPRSKLEV
                     QSFVEDLRDTHDATIVLTTHDMDEAERLCLRIGILNEGRIVAEDTPERLKALVAADHG
                     QEPTMHNVFMTYTGRSLDDDVEEETEDEEE"
BASE COUNT         1001 a         2272 c         2157 g         1024 t
ORIGIN      
        1 tgcggtgggt caccccggtc gaggcgatcg agccgaaggc gctgttcgac tacctccgct
       61 cgcgtcgggg cgcgggcagc gcgagtggcg aattggtcgc cgctccagga gccgcgcggc
      121 gcgtgctcga aggcctccgc caccacgaga tggtggccat cgccgccgac cgtgacgtgg
      181 gcaccgccac ggtagaggtg gagttctttg gccacccggc gcgcatgccc tccggacccg
      241 ccacgctcgc gctgctcacc gggtcgccgg tcgtgatcat caccctgcgc cggaccggcc
      301 acgggcagtt cgtggcctgg agcgagcaga tcccgtggac tgccaccggc gaccggccgg
      361 ccgacatcca ggccctgacc cagcgcatca ccgacacgct ggcgcgtcac atcagcgagg
      421 cgccggagca gtggtgggga tcgtttcagc cggtgtggga cgacctgacc cccgggacgg
      481 tccaggcctg atggaccgat ccccctcggc tggagaccgc ggcaaggccg acctccacct
      541 ccacaccctg tactccgacg ggaccgcacg cctggtcgat ctgctggact ggatcgagca
      601 tcggaccgat ctcgacctcg tcgcgatcac cgaccacgat cggatcgacg gcgcgacccg
      661 cgccgcggcg atgcacgcgg cgggcgacta tcggttccag ttcgtgatcg gtgaggagat
      721 cacgacccgc agcggccacg tggtggggct gttcctcgag gagcggatcc cgccgttccg
      781 gtcggtggcc gagacgatcg agcggatcca cgcccagggc gggctcgcgg tcgcggccca
      841 cccgctcggc gtcttcctct cgctcgggag cggcagcctc ctccgcctcc agtcggaccc
      901 gaacccgcag cgccacctgg acgcggtgga gctcatcaat cccagccttg ggggccgact
      961 tcgccacggc gccctggccc gtctgaacgc cgagcggctc catctgcccg ggatgggcaa
     1021 ctcggacgcc cacgtgctcg agacggtggc cagcgcctgg acctggttcc ctggatcgag
     1081 cgcggatgac ttccgtgcgg ccctggacgc gggcctcgtc gagccgggcg gcagccactg
     1141 gagcacgggt cataacgtgg ccgtctacgg ccgtcagctg atggccaagg cccgaaagct
     1201 cgggcatact ctccgcccgt ctggagagtg gcgatagacc gtgattgagg cccccgaggc
     1261 gacgggccgc gacgctcgcg tggagccgtc cagcccgccg gcgccccgtg cgccgctgaa
     1321 gatcggcatc gtcagcccgt atggctttcc ccatccgggc ggcgtgaacg agcacgtccg
     1381 gcacacgtat gaagagctgc gctcactcgg gcacgacgcg tggatcatca cgcccaagta
     1441 cggcgccgag cgcgacagcg agggacgcgt catccggctc ggcaccggct gggcggtccc
     1501 gagcaacggg agcgtgggcc ggctgaccct gggctggcgg ttgcgacctc ggatccgtga
     1561 ccttctggcc gcccatcggt tcgacgtcct ccattaccac gagccattcg tgccgttcct
     1621 gggacccgcg gtcctcagcg agtcggaaac ggtgaacatc gccaccttcc acgcgttcgg
     1681 tggattctcc ccgtcgtatt gggtgggaag ccgggggacg acccgcctga ccgcgcgtct
     1741 ccatgggcgg atcgcggtca gcggtgccgc ccgccacttc atcggccgct atttcccggg
     1801 cgactaccgg atcatcccga acggcgtcga gatcgagcca ttcgccagcg cgcagccatt
     1861 cgaggagctg cgcgacggca cgctgaacat cctgttcgtg ggcaggctcg aggaccggaa
     1921 gggcctcacc tacctactcc gcggctacca ccggctgcgg aagcgccacg tggacgcccg
     1981 gctgatcgtg gtcggcgacg ggccgcgcct ccgcgagtac cgccgatacg tggccacccg
     2041 gcggatccgc gatgtcgagt tcgtgggcca cgttccgctc gacgccaagc tccgctactt
     2101 cgccagcgcg gacatcttct gcgcgccctc gaccggccag gagagcttcg ggatcgtgct
     2161 cctcgaggcg atggccgccg gcctcccaat cgtggcctcg gacatccacg gctacaagca
     2221 ggtggtgtcc cgggacatcg agggctatct cgtcgagccg cgcaacccgc gcgccatcgc
     2281 cgccgccctg tataagctcg cgcgcgaccc agagctccgc gaccgcatgg gcgaggcggg
     2341 acgggccaaa gcgcccgagt actcctggga acgggtcacc gagcaggtgg tcgcgtacta
     2401 ccacgaggtc cgcgaccggg tcctggccta gacggtctcc tgcggcgcct cggcctcgtg
     2461 gaacgggcgc cggtacgccc gctctcgctg gtggacgacg aagccgttcg cctcgtacac
     2521 gcccaacgca ccggtcgggt tgtcggcgtc cacgccgagg acggcatcgg tcatgccccg
     2581 ctcgcggaag gcgaccaggc tctgggacac gagcgcgcgg gcaaggccct ggcgtcgcca
     2641 tggccggcgg acgctgaccc cggccagcaa ccctcgccgg cctccggtct gctggttgaa
     2701 ctcgcgcatg accatgttgt tgacctggcc cgccacctcg tccccggccc acgccacccg
     2761 ccacagctca ggctcgaagt ccgggccgct gaagaagcga cggaacgcct gctcggacag
     2821 gtccatcccg ccccagtgat cggcgaacgc ctcggtatcc gcattccaga tcgtgcggtg
     2881 gtcctccggc cgaaccggtc ggacctcgat tccagacggc agcgagaact cctcgatgtc
     2941 atccaggttg ggccggagca tctcgaagaa gtagcgcgcg gtcgtgaacc cctcggacgc
     3001 caggagcgcc atggccccga cctgggtctc attgcaccag gtttcgaacc gctggtcggt
     3061 gcccgcgcgg acgtcctggc cggcttcgat ctcccgcagt cggcgctggt tggcatgcag
     3121 gatggcgcgg cccagccctc gccggcgcca ggcgggatgg accgatcccc acagcgcgta
     3181 gttccgaccg ccgtcggtgt cctcggtcca gtggaccttg ccgtagccga tgagcgcccc
     3241 atcgacctcg gccagcagga catcctcgag cgggtcccac gtcgatgcgt gggcaaacca
     3301 ctgggccagt cccgccacgt cgtgacggtc ctcgatctcg tcggcctcgt ccgaggtgtt
     3361 gaacagcccg gcgatcgcct ccaggtcgtc tggcaggcgc atccggcgaa aggccagacc
     3421 cgcgatcggc ggcgcatccg cgacgttcac ccgcacgggg gcgatggtca tcggttcacc
     3481 tcgaacggtc gccgataggc ggtgaagcgc tcggtgacga cgaatccgtt gtcggtgtag
     3541 acgccgagcg cgccggtctc gttgtcggcg tccacgccaa ggacgccctc ggccattccc
     3601 tggtcgcgga gcacgaccag gctgcgagcg accaacgccc gggccaggcc gcggcgacgc
     3661 cacgggcggc gggtgaacac cgagtccagc cagccgtgct tcaccccgag cttggcgttg
     3721 gcctcgtgat agatggcgtt gatcgacgcc ccagcgatct cgtcgccgtc gaacgccacg
     3781 acatggagcg acgggtcgaa gtcgtcgtcg gcgatccact tctggaacgc ggcgtccgag
     3841 ctgtcgatcc cgccccagtg gtcgaggaag gcctcgttgt cggcgatgaa caaccgatgc
     3901 gcgtcatccc gggacaccgg tcgcacctcg atcccatccg gcagcggcgg cacctcgatt
     3961 ggctggctca ggtcgcggtt catgtggaag aaccaccgca ccggcgcgta gccatgctcg
     4021 gaatacaggg cctgcgcgcc gaggtccttc tcggatgccc agctgccggc cacgcgcggc
     4081 cggtcgacgt cctggccggc tgccagctcg tcgatccgtt cccgcgcgaa ggcaaagagg
     4141 gtggtgccga tccggcggcg acgccaggtc gggtcgacct cgcaccagga tcggtactcc
     4201 cgcaggccgt cgttggtgtc gatccactcg accttgcaga agcccaccac ggttccgtcc
     4261 acctccgcca cccgcatctc ggtggcgggg tcgaacttct cgtccgggcg caactccaag
     4321 ccgatctgct gctcggtcat ccgctcctgg aattggtccg ccgcgaacgc cgcattcgcg
     4381 acgcgggtca gctccgggat gtccgcctcg cccgcgaacg ggcgaagctc gatccggagc
     4441 ccggtatcgg tcatgtcagc cattgtcagc ccaccctctt gaggcggccg gtccgcttgg
     4501 cgtaccgctc cgcgcgcccg aaggcccaca ggcccagggg aatgaagacc accgccatga
     4561 tgagcagtgg ccagacgtca tggatcagct ggtcgatccc caccccgtca atcagccccg
     4621 cccgcacccc gtcgaggacg tatgtcgccg gcgagaactg gctgatgaac tgcatccact
     4681 cgggcaggac ctcgatgctg tagtagaccc cgctcaccag cagcaggacc gactggagga
     4741 cgaacaccat ctgcgccccg cgctcgacgt acatgagcgg gaggatggag gccgcgatcc
     4801 cgatcccgat gaagctgatc gtgcccagca gggtgaacgc gatcaccgtt gcccagttcg
     4861 cctgcgagag gtcgagctcg aagaacaggg ccagcgccag caggacgaac agggtgtgga
     4921 ccaggccgaa gatgaccgcg tagaaggcgg ctccgagcag ctgggcatag cgccggatgg
     4981 gcgccatgaa cgtgtactca agtgtgcctt cccagcgctc ccactggacc gcttcggcga
     5041 tgaactcgaa gacgatggcc aggtagctcc agaagatggt gccgatgacc aggacgagga
     5101 gcaggtgctc atcgcccagg ctctcgccga tgagggtgac cgccagggcg cccgccaccg
     5161 aataaacgag ccaggcgact tcccagcccc agtagcgctt ggtgaggttg aagttgcgct
     5221 cgacgaaggc catggaggcc ctcaggtcgc gcgtcagcat ggccatcgga ctattcctcc
     5281 tcgtcctcgg tctcttcctc gacgtcgtcg tcgagggacc gtccggtgta ggtcatgaac
     5341 acgttgtgca tggtcggctc ctgcccgtga tccgccgcga ccagcgcctt taggcgctcg
     5401 ggcgtgtcct cggccacgat ccggccttcg ttgaggatcc cgatccgcag gcacaggcgc
     5461 tcggcctcgt ccatgtcgtg ggtcgtcagc acgatcgtcg cgtcatgcgt gtcgcgcaga
     5521 tcctcgacga agctctggac ctcgagcttg gaccgtgggt cgagcccggt cgtgggctcg
     5581 tccatcagga gcaggatcgg actggtcagg agggcgcgcg cgatggccac cttctgctgc
     5641 atgcctcggc tcagctgctc aacgggccgg cccagacgct tctcggcaat gccgagccgc
     5701 gacaggatcc ggatggcctc ggctttcgcc tccttcgcat ccatgccgta caggcgggcg
     5761 gcgtaggcca ggttctcgaa cgggctgagc ttcttgaaga aggccgcgtc cacgctgacc
     5821 cggttgatca tccgcttgac ggccatctcg tcgcgctcga tgtccttgcc gaagacctct
     5881 accttccccg tgtcgagggt gagcagggtg gagaacagcc ggatgagcgt ggacttcccg
     5941 ctcccgttcg cgcccagcac gccatagatc tcgccacgct cgatacgcag cgagacgtcg
     6001 ttgatggcca tcaccggtcg cttcttgcgc ccgacctgga accgcttggt gacgtgctcg
     6061 gccagcaggg cggggagcgc cgtgggcgta gcctcggagc gggcaggcgc ggcgacccgc
     6121 ggctcctccg ccgtcacggc gaggcgctca tcggtcatcg tcttggcctg tgtgtcgagt
     6181 ccggtctgca tctgtggttc ctgtctggct gaaccgatgg cttccgtttg accgatacgg
     6241 accgggcaac aaaagagccg tggtccggac cggtcccacg gcttggtgct tgtcggcggt
     6301 tgcccgccgg aatcagctgg tcgtgggcat agatctcccg tttcgcggat cgttggaaac
     6361 cgagcgtgtc atgcggaagc ggctcctcgt tgcgaacggg aaggatggca ggtggcggct
     6421 acacccggcg ctggaacgcc cgcggagtct acgg
//
LOCUS       DASYGU010000272         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1484883, whole genome shotgun sequence.
ACCESSION   DASYGU010000272 DASYGU010000000
VERSION     DASYGU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484883"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VGO32_07870"
     CDS             <1..1309
                     /locus_tag="VGO32_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200704.1"
                     /translation="GASALALTAWWSRESRRARRPAIVRPSGAAEDRDRQAHERATAA
                     RDALAAMLRERGVEPGVDPRPAYDGYLAACREREAAAGAAAGAEALRRELESARAAEQ
                     LAADAARATAGAEAGLRAALAAAEPDADAQAPIGDVVARLEAWQAARVDEAVQAERTI
                     REYEELTGLLDGKSVAELAGLAARAEDRAAGLAAAVGPGAAALEGDEQAATDDVEQRR
                     EAAASMSGAMDVREADLVDVAAAEELAIAAHGRVSTVTEFASVIDETLALLEAAQRQV
                     HRDLAPVLATAIREWLPTLSGGAYVEAGVNPADLSIEVKEAATGAWRQAKLLSEGTRE
                     QIYLLLRLAMAQHLVTTGESAPMLLDEVTAQADADRKRAVLDMLQALALERQLVLFTH
                     DDAVLNWAESQLRGDNDRVIRLPASTDASAASSPVSVSIGSGA"
     gene            1384..3357
                     /locus_tag="VGO32_07875"
     CDS             1384..3357
                     /locus_tag="VGO32_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV7200705.1"
                     /translation="MVLAALLPPAQVIGGSTPAPSATEPIQPRQAVERFVTLWRIGDY
                     ERMHEVLATADREHYTEAGFIALHQRFAAAIGLTGMHVAIGSAQATARPSEARPPDAP
                     APNASPVPSSANESPAPTIDPGGVVVGPVRAMAVPVRLTFDSSLFGPIVLDRAFELTL
                     GPTGWEVRWTPESLFPELGTTGGAFRVEQSPLPTRGKIIAADGTVLALTRSDGVRVYP
                     HESLAGQVIGYATPLTAEEAAARPAVDGYAAGQVAGRRGVEAGAEALLRGRAALTLIV
                     DPHLGTDQPVVERPLIPAADVRLTIQPDLQRSAEAAIAGYPEAATAVVDPRSGDVWAL
                     ASAPAFNPNAMTLGTTYGGSPLSRPTAAQIQNHALETAYPTGSSFKPFTLATALQIGV
                     ASPATRMTCLPTWDFGGVRFVNFELHQLPGLVSLEQAMAFSCNTTYMPLSMVVYEHDR
                     DALTDLLADFGFGQPTGIGWVPETGGVLPDALYYEGHRRGSGSYSPFGPFDQVQLAIG
                     QGSFLGTQLQMALAYAAFANRGTLWMPRLVTTAMTPDGDVVHRTRPQVRQRIAMDRAD
                     LDYLVTALQAVTTYSYGTGTAAFAGFGIPVAGKSGTAESGGPDPHALFPAFAPALNPE
                     IVVTTILVRIHLGTGGSDSAPLVRRVMTTYFSR"
     gene            3504..>4635
                     /locus_tag="VGO32_07880"
     CDS             3504..>4635
                     /locus_tag="VGO32_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7200706.1"
                     /translation="MTSREALPLRFQTAEVGVDWLQCNIECQEGCPVNTNCRGYLMLA
                     AEGRFEEGYILARDPNPVAAICGYVCSAPCEKACRRADIDKPLAIRAMKRFLVDWHYA
                     NNMPDDIQVAPATGKSVAVIGAGPAGLSAAKELAGWGHEVHCYDALPSGGGTTLIGVP
                     AFRLPRDVIELDVNWVAKHGVEFHYNVEIGRDITMDQLRAQHDAVVVATGCMYPVAMN
                     VPGEEQDGVIYGVDFLKRANLGQEQWVGDHVVVVGGGYTAMDSSRTALRLGAKTSTIA
                     YRRGPDEMVVDEEEQHETRFEGVQFEFFASPVEVVADDKGKVTGMVFQRVRLGAPDAS
                     GRRSPEPIPGSDFTIPCDMVIPCTSQASDNNVLGEFGSKLNRH"
BASE COUNT          691 a         1692 c         1607 g          645 t
ORIGIN      
        1 cggggccagc gcgctggcgc tcaccgcgtg gtggtcccgc gagtcccggc gtgcccgtcg
       61 ccccgcgatc gtccgtccgt cgggcgccgc cgaggatcgg gaccgtcagg cgcacgagcg
      121 cgcgacggcg gcacgagacg cgctggcggc catgcttcgt gagcgtggcg tcgaaccggg
      181 cgtcgatcca cgccccgcgt atgacggtta cctcgccgcg tgccgcgaac gggaggcggc
      241 cgccggcgcg gcggccggtg ccgaggcgct ccgacgggag ctggaatcgg cccgcgcggc
      301 cgagcagctc gctgccgatg cggcccgcgc gacggccggt gccgaggccg ggctgcgtgc
      361 cgcgctggcg gccgccgagc ccgacgcgga cgcccaggcg ccgatcgggg acgtggtcgc
      421 ccggctcgag gcgtggcagg ccgcccgggt ggacgaggcg gtccaggccg agcgaacgat
      481 tcgcgaatac gaggagctga ccgggctgct ggatgggaag agcgtggctg agctcgccgg
      541 cctggctgcc agggcagagg atcgagccgc aggcctggcc gcagccgtgg gcccgggcgc
      601 cgccgcgctc gagggcgacg agcaggcggc caccgacgac gtggagcaac ggcgcgaagc
      661 cgcggcctcc atgtccgggg ccatggacgt gcgagaggct gacctggtcg acgtcgccgc
      721 ggccgaggag ctcgcgatcg cggcccacgg gcgggtatcc accgtcaccg agttcgcatc
      781 ggtcatcgac gagaccctgg cgctgctcga ggccgcccag cgacaggtcc accgggacct
      841 cgcccccgtg ctggccaccg ccattcggga gtggctgccc acgctgagcg gcggcgccta
      901 cgtcgaggcg ggcgtcaacc cggctgacct ctcgatcgag gtcaaggagg ccgccacggg
      961 tgcgtggcgc caggcaaagc tcctctcgga agggacccgc gagcagatct acctcctcct
     1021 ccggctggcc atggcccagc acctggtcac caccggcgag tcggcgccga tgctgctgga
     1081 cgaggtcacc gcccaggccg acgccgatcg gaaacgtgcg gtcctggaca tgctccaggc
     1141 cctcgccctc gagcgacagc tcgtcctgtt cacgcacgac gatgccgtgc tgaactgggc
     1201 cgagagccag ctccgggggg acaacgatcg cgtcatacgc ctgcccgcct caaccgatgc
     1261 ttcggctgcg tcatcaccgg tcagcgtgtc catcgggagc ggggcctgag catctcgagg
     1321 ggcgcggcgc gctcggtgag gcggctggca cggcgcggaa cggggctcgg actgacgatc
     1381 gcgatggtcc tcgccgcgct gctcccgccc gcccaggtga tcggcggctc gaccccggct
     1441 ccgtcggcca ccgagccgat ccagccccgg caggccgtcg agcgattcgt cacgttgtgg
     1501 cggatcggcg actacgagcg catgcacgaa gtgctcgcca ctgccgatcg cgagcattac
     1561 accgaggccg ggttcatcgc ccttcatcag cggttcgcgg cggcgatcgg gctgaccggc
     1621 atgcatgtcg ccattggcag cgcccaagcg accgcccggc cgtccgaggc gcgaccaccc
     1681 gatgccccag ccccgaacgc gagcccggtc ccctcttccg caaacgagtc gccggcgcct
     1741 accatcgacc ctggcggggt ggtcgtcggt ccggtccgag ccatggccgt gcccgtgcga
     1801 ctgaccttcg acagtagcct gttcgggccc atcgtcctcg accgtgcctt cgagctgacc
     1861 ctaggcccga ccggctggga ggtccgatgg acgcccgaga gcctgttccc cgagctgggg
     1921 acgaccggcg gcgcgttccg cgtcgagcaa agtccgctgc cgacccgcgg caagatcatc
     1981 gccgccgacg gaaccgtcct ggccctcacc cgctcggacg gggtgcgggt ttacccccac
     2041 gaaagcctcg ccggccaggt gatcgggtac gcgacgccgc tcaccgcgga agaggcggcc
     2101 gctcgtcccg cggttgacgg ctacgccgcc gggcaggtcg ccggtcgacg cggtgtcgag
     2161 gccggcgccg aggcgctcct gcgcgggcgg gccgcactca cgctcatcgt cgatccacac
     2221 ctcgggacgg accagccggt cgtggagcgt cccctcatcc ccgccgccga cgtccgcctc
     2281 accatccagc ctgacctcca gcgctcggcc gaggcggcca tcgccgggta tccggaggcc
     2341 gccaccgcgg tcgtggatcc gcgcagcgga gacgtctggg ccctggcctc ggcgccggca
     2401 ttcaacccga acgccatgac cctcgggacc acctacggcg gctcaccgct cagccggccg
     2461 accgccgccc agatccagaa ccacgcgctc gagaccgcat atccgaccgg gtcgtcgttc
     2521 aagccgttca ccctcgccac ggctctccag atcggggtcg cgtcgccggc gacccggatg
     2581 acctgccttc cgacctggga cttcggcggt gtccggttcg tgaacttcga gctccaccaa
     2641 ctgcccggac tcgtctccct cgagcaggcg atggcgttca gctgcaacac gacctacatg
     2701 cccctgtcga tggtggtcta cgagcacgat cgcgatgcgt tgaccgatct cctcgcggat
     2761 ttcggcttcg gccagcccac cggcatcggc tgggtgcccg agaccggcgg ggtgctgccc
     2821 gacgcgctgt actacgaggg ccatcgccgc ggctcgggca gctactcgcc gttcggcccc
     2881 ttcgaccagg tccagctggc catcggccag ggctcgttcc tgggcaccca gctccagatg
     2941 gcccttgcct acgccgcatt cgccaaccgc gggacgctgt ggatgccacg gctggtgacc
     3001 accgcgatga cccctgacgg cgacgtcgtg caccggaccc ggcctcaggt ccgtcagcgg
     3061 atcgccatgg accgcgcgga cctggactac ctggtcaccg ccctccaggc ggtcacgacc
     3121 tattcctacg ggaccgggac cgcggcgttc gccggctttg ggatcccggt cgccggcaag
     3181 agcgggacgg ccgagtcggg cggccccgat cctcacgccc tgttcccggc cttcgccccg
     3241 gccctgaacc cggagatcgt ggtcaccacc atcctggtca ggatccacct cgggaccggc
     3301 ggatcggatt ccgcccccct cgtccgacgg gtgatgacga cctacttcag ccgctgaaaa
     3361 tcggatcgtc gtctttgaca ccgtgagcac gcgacgggta gcatcgaagc gcagaaattc
     3421 gcctcgaaat gccggtccac ggctggaact gacgccgatt gctgggggag ccaaagggga
     3481 cggtcgatga atagcgaacg cccatgacat cccgggaggc gctacccctt cgcttccaga
     3541 cggcggaagt cggtgtcgac tggctgcagt gcaacatcga gtgccaggaa ggctgcccgg
     3601 tcaacaccaa ctgccggggc tacctcatgc tcgcggccga gggccggttc gaggagggct
     3661 acatcctcgc ccgcgatccg aacccggtcg ccgccatctg cggctacgtg tgctcggcac
     3721 cctgcgagaa ggcctgccga cgggcggaca tcgacaagcc gctggccatc cgggccatga
     3781 agcggttcct cgtcgactgg cactacgcca acaacatgcc agacgacatc caggtcgccc
     3841 cggccacggg caagagcgtg gcggtcatcg gtgcgggacc ggccggcctg agcgcggcca
     3901 aggagctggc cggctggggc cacgaggtcc actgctacga cgccctgccg tcgggcggcg
     3961 ggacgaccct catcggtgtg ccggcgttcc gcctcccccg cgacgtcatc gagctggacg
     4021 tgaactgggt tgccaagcac ggcgtcgagt tccattacaa cgtcgagatc gggcgcgaca
     4081 tcacgatgga ccagctgcgg gcccagcacg acgcggtggt ggtggccacc ggctgcatgt
     4141 acccggtcgc catgaacgtg cctggcgagg agcaggacgg cgtcatctac ggcgtcgact
     4201 tcctgaagcg cgccaacctg ggccaggagc agtgggtcgg cgaccacgtg gtggtggtcg
     4261 gtggcggcta tacggccatg gactccagcc ggacggccct gcggttgggc gccaagacca
     4321 gcaccatcgc ctaccgccgc ggtccggacg agatggtcgt ggacgaggag gagcagcacg
     4381 agacgcgctt cgagggcgtg cagttcgagt tcttcgcctc ccccgtcgaa gtggtggccg
     4441 acgacaaggg caaggtcacc ggcatggtct tccagcgcgt gcggctgggg gcacccgatg
     4501 ccagcggccg gcgctcgccg gagccgatcc ccggcagcga cttcaccatc ccgtgcgaca
     4561 tggtcatccc ctgcaccagc caggcgtcgg acaacaacgt gctgggcgag ttcggctcga
     4621 agctgaaccg gcacc
//
LOCUS       DASYGU010000273         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_354196, whole genome shotgun sequence.
ACCESSION   DASYGU010000273 DASYGU010000000
VERSION     DASYGU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354196"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGO32_07885"
     CDS             <1..501
                     /locus_tag="VGO32_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399519.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV7200707.1"
                     /translation="PGEERILVPSRRDVATYDQAPAMSAEAITDQVVVAITGGEYPFV
                     VVNYANTDMVGHTGDWDAALAALEVIDTCLGRVADAATATGAALLITADHGNVERMRD
                     ASGGPHTAHTTADVPLVLVHRGHEAVGLRDGILADVAPTICALLEIPAGPAMTGHSLL
                     ASATTT"
     gene            524..745
                     /gene="secG"
                     /locus_tag="VGO32_07890"
     CDS             524..745
                     /gene="secG"
                     /locus_tag="VGO32_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV7200708.1"
                     /translation="MTALTLVQSILAVALTVAILLQQRGAGVGGAFGGEITAYRSRRG
                     IERTLFRLTIVLATLWVVFSVLALYLQTA"
     gene            854..1402
                     /locus_tag="VGO32_07895"
     CDS             854..1402
                     /locus_tag="VGO32_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200709.1"
                     /translation="MSVSSMANIAFARRADVAPEGEGLNSLDEVASATGTPSGSATSA
                     TSALSAIAAYIPTEVVTVYVAVLATIGVTQPQGNGPAYTPVAVYATFLVLTPVVVWGL
                     YASRAVSAGKRLPRSLRSWPKWEMVAATLAFAAWGAAMPSSPLGIIPGFMPALAGVVA
                     LVLSLLIGVFSPIFSIRPLANR"
     gene            1419..2345
                     /locus_tag="VGO32_07900"
     CDS             1419..2345
                     /locus_tag="VGO32_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200710.1"
                     /translation="MVTVWSEAQPGRPLTDCVYSAGLMALDFAGWNRWPNGHHTIAER
                     NALERSQTMFARNAGSNFAALDQATKKRFGVALRLVSGGLAEALSRPGTVLALGGVNG
                     RLEPGDRWRRWDPTFTDFHAVAVIPLGDGTCRVLDPLAPMGFVGDVVPNSKVLTWANG
                     PSAADARLARAGEFASAAGNVPREEDTVTEVTISVFAEGPRTCFIPAGGRVEGWTLDG
                     LKVSKVWPDGSNFPADAGVFIRQRPQKAPNGTFLRCTAGTYVGSYVPVTHVRLGPPVN
                     PPADCSALEAELAAARAELAAERDRFEAGIKE"
     gene            2531..2618
                     /locus_tag="VGO32_07905"
     tRNA            2531..2618
                     /locus_tag="VGO32_07905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2565..2567,aa:Leu,seq:cag)
     gene            2627..2711
                     /locus_tag="VGO32_07910"
     tRNA            2627..2711
                     /locus_tag="VGO32_07910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2661..2663,aa:Leu,seq:gag)
     gene            2737..2813
                     /locus_tag="VGO32_07915"
     tRNA            2737..2813
                     /locus_tag="VGO32_07915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2771..2773,aa:Val,seq:tac)
     gene            2870..3436
                     /locus_tag="VGO32_07920"
     CDS             2870..3436
                     /locus_tag="VGO32_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV7200711.1"
                     /translation="MEWILLIIVAVIVIGGILIYNGLVRLRNQVDEAWNQISVQLKRR
                     HDLIPNLVNAVRGYMEFEQETLTRVIEARNAAVTASSAGPANAGAAGGAENFLTGALR
                     QLFALVENYPDLKSNQNVMQLQEELTTTENQIGFSRQHYNATVREMNTAIQTFPNVLL
                     AGLLGFKEREYFQIEEADTAVPEVNLQV"
     gene            3459..>3766
                     /locus_tag="VGO32_07925"
     CDS             3459..>3766
                     /locus_tag="VGO32_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV7200712.1"
                     /translation="MSESAPTFYAQIAANRRASWALMLIVTGLLVALAGTAGYALGFE
                     WYGIPFAIILAAILGSTSYFGGDRLVLAASDAREIDRANPPDTLNQLMTVVEEMRLAS
                     G"
BASE COUNT          588 a         1318 c         1245 g          615 t
ORIGIN      
        1 cccggggagg agcggatcct ggtgccgtcg cgtcgggacg tggcgaccta cgaccaggcg
       61 ccggccatga gcgcggaggc gatcaccgac caagtggtgg tggccatcac cggaggcgag
      121 tacccgttcg tggtcgtgaa ctacgcgaac accgacatgg ttggccacac cggcgattgg
      181 gatgccgcgc tcgcggcgct cgaggtcatc gacacgtgcc tgggccgagt tgcggatgcc
      241 gcgacggcca ccggcgccgc cctgctgatc accgccgacc acggcaacgt ggagcgaatg
      301 cgagatgcgt ccggtggccc gcataccgcc cacaccacgg ccgatgtccc actcgtgctc
      361 gtccatcgcg ggcacgaggc cgttggccta cgggacggca tcctggccga cgtcgcgccc
      421 accatctgcg ccctgctcga gatcccggcc ggcccggcca tgaccggcca cagcctgctg
      481 gccagcgcca ccacgacgtg accttggcta tactgccgcc cgcatgaccg ccctcaccct
      541 cgtccagtcg atcctcgccg tggccctgac cgtcgccatc ctgcttcagc agcgtggcgc
      601 gggggtcggt ggcgcgttcg gcggcgagat caccgcctac cgcagccggc gtggcatcga
      661 gcgaaccctg ttccggctca cgatcgtgct cgcgacccta tgggtcgtgt tttccgtcct
      721 ggcgctgtac ctccagacgg cctgaccgat ccgggccaag gccccggtcg taaagggtgc
      781 agccgaccga ccggcgcaca gatacgcggt ctatcctcag acgcgatcag cgcgcaccac
      841 gggaggacgg accatgagcg tctctagcat ggcgaacatt gccttcgcgc gacgggcgga
      901 cgtcgcccct gaaggcgaag gcctcaactc gctcgacgag gtcgcgagcg ctacaggaac
      961 tccgtcgggc tccgccacca gcgcgacgag tgcgctgtct gccatcgccg cctacatccc
     1021 gaccgaggtc gtgaccgtgt acgtcgcggt gctggccacc attggggtca cccagcctca
     1081 gggcaacggg ccggcgtaca cccccgtcgc ggtgtacgcg acgtttctgg tcctcacccc
     1141 ggttgtcgtg tggggcctct acgcgtcccg cgcggtttcg gccggcaagc gcctcccgcg
     1201 ctccctgcgg tcatggccaa agtgggagat ggtcgccgcg actctggcgt tcgccgcgtg
     1261 gggcgcggcg atgcccagct ccccgctggg gattatcccc ggattcatgc ctgctctcgc
     1321 cggcgtggtt gccctcgtcc tatctctgct gatcggtgtc ttttcgccga tcttctcgat
     1381 ccgtccgctc gcgaaccgct agttgggagg gctggcaggt ggtcaccgtc tggtctgagg
     1441 cgcagccggg ccggcccctg accgattgcg tgtactccgc ggggctcatg gcgctggact
     1501 tcgccggctg gaaccgctgg cccaacgggc atcacacgat cgccgagcgg aacgcgcttg
     1561 agcgctcgca gaccatgttc gcgcgaaacg ccggctcgaa cttcgccgcc ctcgaccagg
     1621 caaccaagaa gcgtttcggc gtcgcgctcc gcttggtgag tggcggtctc gcagaggcgc
     1681 tgagccgccc cggcacggtg ctcgccctgg gcggtgtcaa cggtcgtctc gagcccggcg
     1741 atcgctggcg ccgttgggat ccgaccttta ccgacttcca tgccgtcgcc gtgatcccgc
     1801 tcggagacgg cacgtgccgc gtgctggatc cactggcgcc catggggttt gtcggggacg
     1861 tcgtgccaaa cagcaaggtg ctcacctggg cgaacgggcc gtcggccgca gacgcgaggc
     1921 tcgcccgcgc gggggaattt gcctccgccg ccggaaacgt gccaagagag gaggacaccg
     1981 tgaccgaagt cacgatctcg gtgttcgcgg aggggccgcg aacatgcttc atcccggccg
     2041 gaggccgcgt cgagggctgg accctagacg gcctcaaggt ctcgaaggtc tggcctgacg
     2101 gctccaactt ccctgccgat gccggggtgt tcatccgcca gaggccgcag aaggccccga
     2161 acgggacgtt cctgcgctgc accgcgggga cctacgttgg gagctacgtc ccggtcaccc
     2221 acgtacggct cggcccgccg gtaaacccgc cggcggattg ctcggccctt gaggccgagc
     2281 tggccgcggc acgggcggag ctcgccgccg agcgggatcg cttcgaggcc ggaatcaagg
     2341 agtagtcagg gcgtgaatgt gggcgctgag gagaccgacc gtcgggcgga cctcctcccc
     2401 caaagggact gcttgggacg cggcaatgcc cggtgctaga atcgctgcct ggccgcctgc
     2461 tcgcattcat tgatctggat gtgatctggc ggccgcggag tcggccccca aaggctggtt
     2521 ccgagctcgg gccgaggtgg cggaataggc agacgcgcta ggttcaggac ctagtgacca
     2581 ttacggtcgt gtgggttcaa gtccctccct cggtaccaga ctccgggccc aggtggcgga
     2641 attggtatac gcgtacgttt gaggggcgta tgaggcaact catccgggtt caagtcccgg
     2701 cctgggcacc acttcaccgg cggtaggctc cgccgcgggc ggttagctca gctggtcaga
     2761 gcgtctcctt tacacggaga aggtcggggg ttcgagcccc tcaccgccca ccaccagccg
     2821 cgagtaccat gatcggcaca ccctttgtgc cctaggagac gtcgccccca tggaatggat
     2881 cctcctgatc atcgtcgccg tcatcgtcat cggcggcatc ctcatctaca acgggctggt
     2941 tcgcctccgc aaccaggtcg acgaggcctg gaaccagatc agcgtccagc tgaagcggcg
     3001 acacgacctg atcccgaacc tcgtgaatgc cgtgcgcggc tacatggagt tcgagcagga
     3061 gaccctcacc cgggtcatcg aggctcgcaa cgcggctgtt acggcatcgt ccgctgggcc
     3121 cgccaacgcc ggcgcggccg gaggagccga gaacttcctg accggagccc tgcgccagct
     3181 gttcgccctg gtcgagaact accccgacct gaagtccaac cagaacgtca tgcagctcca
     3241 ggaagagctg actacgaccg agaaccagat cggcttctcc cgccagcact acaacgcgac
     3301 cgtccgggag atgaacacgg cgatccagac cttcccgaac gtcctgctcg ccggcctcct
     3361 gggcttcaag gagcgcgagt acttccagat cgaggaagcg gacaccgccg tgcccgaggt
     3421 gaacctccag gtctgaccgt ggcgggccac cgctcccggt gagcgaatcc gcgccgacgt
     3481 tctacgccca gatcgccgcc aaccggcgcg cgagctgggc gctgatgctc atcgtgaccg
     3541 ggctcctggt ggcgctcgcc ggcaccgcag gctacgcgct cggcttcgag tggtatggga
     3601 tcccgttcgc gatcattctg gccgcgatcc tgggcagcac ctcgtacttc ggcggcgatc
     3661 ggctcgtcct cgccgccagc gacgcccgcg agatcgatcg cgccaacccg ccggacacct
     3721 tgaaccagct gatgaccgtc gtcgaggaga tgcggctggc cagcgg
//
LOCUS       DASYGU010000274         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_96503, whole genome shotgun sequence.
ACCESSION   DASYGU010000274 DASYGU010000000
VERSION     DASYGU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96503"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1018
                     /locus_tag="VGO32_07930"
     CDS             11..1018
                     /locus_tag="VGO32_07930"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV7200713.1"
                     /translation="MYSGNANRPLAENICRYLKTRMGEADVFQFLNENIFVRILENVR
                     EQDVFLIQPTCRPVNHSIMELLIMIDAFKRASAGRITAVMPYYAYGRSDKKDQPRVPI
                     TARLVADMLTVAGADRVLTMDLHQGQIQGFFSIPVDELTAVDMLARYVTGLDLDDLVV
                     VSELGFAKKARNFAERLQAPLAIVEKRRTGNDDQAELKNVIGEVEGKTAVIIDDEINT
                     GGSLIETVNALISEGAREVYSCATHGVFSGSAIERIDASALKEVIVTDTIPLPEGTDS
                     ERITVLSVAPLFGEAIRRIHRGESVGALFSSEVELVEEMTFWGAHGEDEEEIEAVTIE
                     R"
     gene            1025..1246
                     /locus_tag="VGO32_07935"
     CDS             1025..1246
                     /locus_tag="VGO32_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7200714.1"
                     /translation="MAELRLGILPWGQATDWESIEAVARRIEELGYEHVWAWDHLHAI
                     FGDPDQPIFEGWMTLAAWAQVTRRVRATN"
     gene            1348..1596
                     /locus_tag="VGO32_07940"
     CDS             1348..1596
                     /locus_tag="VGO32_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200715.1"
                     /translation="MKKLIFGLTAIAGIAGVAALTYQFAVRPFRSWGTSPEQSARALP
                     GDELLPDASGGETRTITIEAPPASVWPWLVQMGYGRGG"
BASE COUNT          300 a          557 c          548 g          253 t
ORIGIN      
        1 cgagttcagc atctactcgg gcaacgccaa ccggccgctg gcggagaaca tctgccgcta
       61 cctcaaaacc cggatgggcg aggccgatgt cttccagttc ctgaacgaga acatcttcgt
      121 tcggatcctc gagaacgtcc gtgaacagga cgtgttcctg atccagccca cctgccgccc
      181 ggtgaaccac tcgatcatgg agctcctgat catgatcgac gccttcaagc gcgcgagcgc
      241 cgggcggatc acggccgtca tgccgtacta cgcgtatggc cgttccgaca agaaggacca
      301 gccccgggtc ccgatcaccg cccgactggt ggccgacatg ctgaccgtgg ccggcgcgga
      361 ccgggtgctg accatggacc tccaccaggg ccagatccag ggcttcttct cgatcccggt
      421 cgacgagctg acggccgtcg acatgctcgc gcgctacgtg accggcctcg acctggacga
      481 cctggtcgtg gtcagcgagc tggggttcgc caagaaggcg cgcaacttcg ccgagaggct
      541 ccaggcgccc ctcgccatcg tcgagaagcg gcggaccggg aacgacgacc aggccgagct
      601 gaagaacgtc atcggtgagg tcgagggcaa gaccgcggtg atcatcgacg acgagatcaa
      661 caccggcggg tcgctcatcg agaccgtcaa cgcgctcatc agtgagggcg cccgcgaggt
      721 ctactcctgc gcgacgcacg gcgtcttctc cggctccgcc atcgagcgga tcgatgcctc
      781 cgccctcaag gaggtcatcg tgaccgacac gatcccgctt cccgagggca cggactcgga
      841 gcggatcacg gtcctgtccg ttgcgcccct gttcggggaa gcgatccgac gcattcaccg
      901 cggagagagc gttggtgcgc tcttctcgtc agaggtcgag ctcgtcgagg agatgacctt
      961 ctggggtgcc cacggcgagg acgaggaaga gatcgaagcc gtcacgatcg agcgctgacc
     1021 ggccgtggca gagctgcggc tggggatcct cccctggggg caggccaccg attgggagtc
     1081 catcgaggcg gtggcgcgcc ggatcgagga gctcggctac gagcacgtct gggcctggga
     1141 ccacctgcac gccatcttcg gtgacccgga ccagccgatc ttcgaaggat ggatgaccct
     1201 cgccgcgtgg gcacaggtca cgcggcgggt gcgggccacg aactgagcga gcgggccatc
     1261 gggccgtgcg catcgcgagt ggcacttgcg cccgcgccca attcagggga gcctgaaggc
     1321 acacatccaa agacctcgag gatcccaatg aaaaagctca tcttcggcct gaccgccatc
     1381 gcaggcatcg ccggcgtggc cgccctgacg tatcagtttg ccgtgcgccc gttccggagc
     1441 tgggggacca gcccggagca atcggcccga gccctgcccg gcgacgagct gctccccgac
     1501 gcctcgggcg gtgaaaccag gaccatcacc atcgaggcgc cgccggcatc ggtgtggccg
     1561 tggctggtgc agatgggata cggccgtggc ggctagtaca gcttcgacgc catggatacc
     1621 ggcgccagca cgaacgcgat cctgcccgaa ttccagtc
//
LOCUS       DASYGU010000275         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_250988, whole genome shotgun sequence.
ACCESSION   DASYGU010000275 DASYGU010000000
VERSION     DASYGU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250988"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VGO32_07945"
     CDS             complement(<1..1274)
                     /locus_tag="VGO32_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV7200716.1"
                     /translation="MDDLTIDLPTGVRHALGTLTGAGHEAALVGGCVRDLVRGLQPGD
                     WDLTTSAPPEAVAALFPDTTWTNIFGTVTVRLEDDPMEVEVTTYRSESGYTDGRRPDQ
                     VTWGTDVAEDLQRRDFTINAMAWVADDLAAGRGHLVDPYGGRQDLAAGLLRAVGDPDR
                     RFTEDALRLLRGARFATTLGLRIEPATEAAMNRLAPTVARLSGERIRDELFRLLAWDG
                     PTAPAFEQMERFGILGEILPELAALRGVEQGKLLGGDALDHSLRAADALPANDPLLRL
                     TGLLHDVGKAPTASGGHFIGHEVVGAQLVEHRLEALALPRADITRATHLVRHHMILYG
                     PEWTDAAVRRFIGRVGPGQPMADLFALRRADTAASAGPEARDAAAAELEARVAALQGS
                     AVLTVGELAVNGSDLMRELNLAPGPEVGRLLDV"
     gene            1792..3774
                     /locus_tag="VGO32_07950"
     CDS             1792..3774
                     /locus_tag="VGO32_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEV7200717.1"
                     /translation="MAWAEPEYRRSPSAAPEGEPLYPQQWGLENEGQDIGGFPGAPNV
                     DMNLPEAWELGLGDPSVTVAVIDTGVDFSHPDLQGTAWTNPGESGGGKETNGLDDDAN
                     GYIDDVNGWDFCHDDKTVFEAVDGWHGTAMATIIGGSANGTGMTGVAPGVRIMALKIL
                     DASGDCGVAAEIAAIQYAVDQDVKIANASYGGGGYSEAERLAIWDASGSGMLFVAAAG
                     NEGADLEIEPSYPAAYDIDNLIAVAAVHNEGMLSGCGGGACSNYGLNVDIAAPGESIL
                     VGATLNQPDPHVWALVSGTSPAAANVSGVAALIGSVRPELFTGDLLKVQLLKTGKPVP
                     WTRGLTASGRIPDARAALIASPDIVRLSGPNRYATAAEISASTYFRYPPFVLVANGSG
                     FPDALAGGAVGAQFGYPVLLVKPGSIPPETAAELTRLRPLNIAVLGGTGVVSGAVEAQ
                     LEAFVEPGGSVIRLAGADRFETSVAISQGFDPGVTAVFVATGMDFPDALGAAPPAALL
                     GGPLLLVRKDSIPPVVAFELDRLAPSYIYVLGGTGVVSQSVEGTLWSFTNQGTVFRRA
                     GSNRYETAVSISQGFYPPETGTVFVATGLNFPDALAGGSAGGAFLGPLLLVPGTSVPA
                     AVETELQRLNPSRVFILGGTAVVSDSVITEIENLFP"
     gene            complement(3795..4322)
                     /locus_tag="VGO32_07955"
     CDS             complement(3795..4322)
                     /locus_tag="VGO32_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7200718.1"
                     /translation="MINAPVLVLNQNYEALNVCDIRRAFRLLGAEKAELIARNHQVIH
                     TPTRAYEAPSVIRLVYLVKRPQPRVKLSRREVFARDRHTCQYCGMVTHDLTIDHVVPR
                     HRGGKHEWENLVAACRRCNHRKGSKTLAEAHMYLGREPRAPRADARYLYGSYLSDERN
                     DAWRTFLFVDEPARN"
     gene            4373..>4895
                     /locus_tag="VGO32_07960"
     CDS             4373..>4895
                     /locus_tag="VGO32_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV7200719.1"
                     /translation="MTTTERPPQTSTWATKVGLARMLAGGVIMDVVTADHARIAEEAG
                     AVAVMALERVPSDIRKHGGVARMSDPKLIEEIKQAVTIPVMAKCRIGHFVEAQILQSL
                     EVDYIDESEVLTPADEAHHIDKHQFAVPFVCGCRDLGEALRRIGEGAAMIRTKGEAGT
                     GNIVEAVRHMRAVR"
BASE COUNT          758 a         1668 c         1719 g          750 t
ORIGIN      
        1 acgtcgagga ggcggccgac ctcgggtccc ggagcgaggt tgagctcgcg catgaggtcg
       61 gaaccgttga ccgcgagctc cccgacggtg agcacggccg atccctggag cgcggccacc
      121 cgagcctcga gctcggccgc cgccgcgtcc cgcgcctcgg ggccggcgga cgccgcggtg
      181 tccgcccggc gcagggcgaa caggtcggcc atcggctgac cgggaccgac acggccgatg
      241 aaccggcgca cggcggcatc ggtccattcg gggccgtaca ggatcatgtg gtggcggacc
      301 aggtgggtgg cccgcgtgat gtcggcgcgg ggcagggcca gcgcctcgag ccggtgctcg
      361 accagctggg cgcccacgac ctcgtgaccg atgaagtggc cgccgctggc ggtcggggcc
      421 ttgcccacgt cgtgcagcag gcccgtcagg cggaggagcg ggtcgttcgc cggcagtgcg
      481 tccgcggcgc gcagggagtg gtccagcgca tcgccgccga gcagcttgcc ctgctccacg
      541 ccgcgcaggg cggccagttc gggaaggatt tcacccagga ttccgaaccg ctccatctgc
      601 tcgaacgcgg gcgccgtggg cccatcccat gcgagcaggc ggaacagctc gtcccgaatc
      661 cgctccccgg agaggcgcgc gaccgtcgga gccagccgat tcatggcggc ctcggtcgcc
      721 ggttcgatcc tgaggcccag ggtggtcgca aaccgcgcgc cgcggagaag gcgcaaggcg
      781 tcctcggtga accgccggtc cgggtccccg accgcgcgca ggaggccggc tgcaagatct
      841 tggcggccgc cgtacgggtc gacgaggtgg ccgcgcccgg cggcaaggtc gtccgcgacc
      901 cacgccatgg cattgatcgt gaagtcacgc cgctggaggt cctcggccac gtcggtcccc
      961 caggtgacct gatcaggccg gcgaccatcc gtgtaccccg actccgaccg gtaggtcgtg
     1021 acctcgacct ccatcggatc gtcctcgagt cgcacggtga ccgtcccgaa gatgttcgtc
     1081 caggtcgtgt ccgggaacag cgcggccacc gcctcgggcg gggccgaggt ggtcaggtcc
     1141 cagtcgccgg gctgaaggcc acggaccagg tcccggacgc agcccccgac gagcgcggct
     1201 tcatgcccgg ctccggtcag ggttccgagc gcgtggcgca cacccgttgg caggtcgatg
     1261 gtcaggtcgt ccaccgaccc gatcctgcgt gttcggcctg gcaacggcaa cttggtattg
     1321 ctatcggaaa tcgttaggtg tatatcgggc cggctgaagc ggccggggca gggctgcggc
     1381 cccgtaccga taggagctct gttggcccga cggcgagcct tccaccaccg agtcgcgacc
     1441 atcatgctgg tggggctctg cgttggaccg tttgcagcgc cccagctggc gcatgcggga
     1501 gacccccagg gtgcctcttg ggacgccggc ctagcgattg ggagttctcc cggcgcagtg
     1561 tcgaacgggc ggaggttgct gccggagtcc cgaccaggcc cgcacgcaga gacaaccggg
     1621 cgcgtactgg tccaatggcg accgctcgcg acgcagagtc agcggctgac actgatcaac
     1681 cggacaggcg ctgttgaata tcttgccgcg ccggcactcg atcattggga ggtcgtcacc
     1741 ccgtctggag acttcgacca gacgctggcc gccctgcgac gggatccagc cgtggcgtgg
     1801 gcggagccgg agtaccgaag aagtccttcg gccgcgcccg agggcgaacc tctataccca
     1861 cagcagtggg gcctcgagaa cgaaggacag gacatcggtg gattcccggg cgcgcccaac
     1921 gtggacatga atctgcccga ggcttgggag ctcggactgg gggatccctc agtgacggtc
     1981 gccgtcattg acaccggcgt cgacttcagc cacccggatc tccagggcac ggcgtggacc
     2041 aacccgggtg agagcggcgg aggaaaggaa acgaacggac tcgacgacga cgccaacggc
     2101 tacatcgacg acgtgaacgg gtgggatttc tgccacgacg acaagacggt cttcgaagct
     2161 gttgacggtt ggcacggcac ggccatggcc acgatcatcg gcgggagcgc caatggcacc
     2221 gggatgacgg gcgtcgcccc cggcgtgcgg atcatggcct tgaagatcct ggacgcaagc
     2281 ggagattgcg gtgtcgcggc agagatcgcg gccatccagt acgccgttga ccaggacgtc
     2341 aagatcgcga acgcgtcgta cggcggcggc ggctacagcg aggccgagcg cctcgccatc
     2401 tgggacgcat cgggttccgg catgctgttt gtggcggcgg ccggcaacga gggggccgac
     2461 ctggagattg agccgagcta cccagccgca tacgacatcg acaacctgat tgccgtggcc
     2521 gcggtgcaca acgaggggat gctgtcgggc tgtggcggcg gagcctgctc gaattatggc
     2581 ctgaacgtcg acatcgccgc ccccggagag tcgatcctcg tcggcgcgac gttgaaccag
     2641 cccgacccgc acgtctgggc gctggtctcg ggcacctcac ccgccgccgc gaacgtctcg
     2701 ggggtcgcgg cgctcatcgg ctcggtccga ccggagctct tcaccggcga cctgctgaag
     2761 gtccagctcc tgaagaccgg caagcccgtc ccgtggaccc gcggcctgac ggccagtggc
     2821 cggatccccg atgcgcgcgc ggctctcatc gcttcaccag acatcgtgcg gctgagcggc
     2881 ccgaatcgtt atgcgaccgc ggccgagatc tcggcatcga cctacttccg ctatccgccg
     2941 ttcgtcctcg tggccaacgg atccgggttc cccgatgcgt tggccggcgg tgcggttggc
     3001 gcccagttcg gctatccggt gctgctcgtc aagcccggga gcatccctcc ggagactgcg
     3061 gccgagctca cgcgcctgcg gcccctgaac atcgcggtcc tcggtggcac cggcgtggtg
     3121 tccggggcgg tcgaggcgca actcgaggcg ttcgtcgaac cggggggcag cgtgatccgg
     3181 ctcgcgggtg ccgatcggtt cgagacgtcg gtcgcgatct cccagggctt cgaccccggc
     3241 gtgacggcag tgttcgtcgc cacaggcatg gactttcccg atgcgctcgg cgctgcgccg
     3301 cctgccgccc tcctgggcgg accgcttctc ctggtcagga aggattcgat cccgccggta
     3361 gtcgcattcg agctggaccg attggctccc tcgtacatct acgtcctcgg cgggacaggg
     3421 gtcgtctccc aatccgtcga gggcaccctc tggtcgttca ccaatcaagg caccgtgttc
     3481 cggcgggcgg ggagcaaccg ctacgagacc gccgtcagca tctcgcaggg cttctatccg
     3541 cccgagacgg gaacggtctt cgtcgccact gggctgaact ttcccgacgc acttgccggc
     3601 ggatccgcgg ggggagcctt cctcgggccg ctcctgctcg tcccaggcac cagcgtgccg
     3661 gccgcggtgg agacggagct ccagcgtctc aacccctccc gagtgttcat tcttggaggg
     3721 acggctgtcg tctcagacag cgtgatcacc gagatcgaaa acctgttccc ctgaggctgc
     3781 cccggtcgcc cgtcctagtt gcgggcgggc tcgtccacga acaggaacgt ccgccacgcg
     3841 tcgttgcgct cgtcggacag gtaagagccg tacaggtagc gcgcatccgc ccgcggcgcg
     3901 cgcggctcgc gacccagata catgtgggcc tcggccaggg tcttcgaccc cttcctatgg
     3961 ttgcaccggc ggcaggccgc cacgaggttc tcccattcgt gcttgccgcc acggtgacgc
     4021 ggcacgacat ggtcgatggt gaggtcgtgg gtcaccatcc cgcagtactg gcaggtgtga
     4081 cggtcgcggg cgaacacctc gcgccggctg agcttcaccc ggggctgcgg ccgcttgacg
     4141 aggtacacga gccggatgac cgacggcgcc tcgtacgcgc gggttggggt gtgaatgacc
     4201 tgatggttgc gggcgatcag ctcagccttc tcggccccga gcagccggaa cgcccgcctg
     4261 atgtcgcaga cgttgagggc ttcgtagttc tgattgagca ccaggaccgg cgcgttgatc
     4321 aatctccgcc tccagtggcc gcgatcatag ccgagcgtac aatgcgggcc tcatgacgac
     4381 caccgaacga cccccgcaga cctcgacctg ggcgaccaag gtcgggctgg cccgcatgct
     4441 cgccggcggc gtgatcatgg acgtcgtcac cgccgaccac gcccgcatcg ccgaggaggc
     4501 gggggccgtg gcggtgatgg ccctcgagcg cgtcccgtcg gacatccgga agcacggcgg
     4561 cgtggcccgg atgagcgacc ccaagctgat cgaggagatc aagcaggcgg tcaccatccc
     4621 ggtcatggcc aaatgccgga tcggccactt cgtggaggcc cagatcctcc agtcgctcga
     4681 ggtggactac atcgacgagt cggaggtcct gaccccggcc gacgaggcgc accacatcga
     4741 caagcaccag ttcgcggtcc cattcgtgtg cggctgccgc gacctgggcg aggcgctgcg
     4801 ccggatcggc gagggggcgg ccatgatccg gaccaagggc gaggcgggca cgggcaacat
     4861 cgtcgaggcc gtccgccaca tgcgcgccgt gcgat
//
LOCUS       DASYGU010000276         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_662716, whole genome shotgun sequence.
ACCESSION   DASYGU010000276 DASYGU010000000
VERSION     DASYGU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662716"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VGO32_07965"
     CDS             complement(<1..1626)
                     /locus_tag="VGO32_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV7200720.1"
                     /translation="MTAVQPVPADRLLAEAEAQYPHWELVRDVVDEVIDLALNHRQSG
                     HPGGSRSKVHLLVSLMLSGAMRWDIRRPWLPFADRFVLSAGHTVPLIYATLAVLNETL
                     RARADRDPDERYALPDGGKWAIGWEDLLLLRRRGGLPGHAEMEGKTLFLKWNTGPSGH
                     GMPPSLGQAVALKLAGAEEVKVFVVEGEGGLTPGASHETRNSAWGLGLSNLVFLLDWN
                     DYGIDDPAISSVVPGTPQSWFEAYHWRVNGTMEGSEWGPVTAAVLEAARGENPERVPS
                     AAWFRTRKGRGYGKYDNKSHGTPWPMNAPEFWAVRHEFMERHGVTYAGVDEPAPADAA
                     ERTAQSRHNLEVAIGVLRRHTEVVDAISDRLVEVAGLVPERVKGFNLGGRGAEIFDDP
                     RFTDVSAYPDSFWKAPGEKVANRAAFASWGAWVNATAKADYDRPLFIVCSADLAESTN
                     IAGFAKGHEDLPGWGWYERSSNLRGALLPQQITEFTNSGLTVGLASVNLSDDPMTSFN
                     GFWGACSTYGSFSYLKYGPMRLFSQLAQDCQLKV"
     gene            complement(1623..>2244)
                     /locus_tag="VGO32_07970"
     CDS             complement(1623..>2244)
                     /locus_tag="VGO32_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7200721.1"
                     /translation="GRTPAWVLTDEVYSRITYDVEAPSIVTQPGMLERTIVLDGFSKT
                     FAMTGWRLGYGIIPTWMAPTYSRLVINSVSCTNAFAQAGAIAALTGPQDAADAMRTEF
                     IARRQLIVDGLNAIPGVSCVMPHGAFYVFPNVSSFGRSSTEIATHLLYDGGVCGLAGT
                     AFGRHGEGYLRFSYANSRENLSAALERITDSLARLERSGELVTAAG"
BASE COUNT          338 a          773 c          795 g          338 t
ORIGIN      
        1 caccttcagc tggcagtcct gggccagctg cgagaacagg cgcatcggtc cgtacttgag
       61 gtacgagaac gagccatacg tcgagcacgc gccccagaac ccgttgaacg aggtcatcgg
      121 gtcgtccgac aggttgaccg aggccaggcc cacggtcagg ccgctgttcg tgaactcggt
      181 gatctgctgc ggtaggagcg cgccgcgcag gttcgagctg cgctcgtacc agccccagcc
      241 gggcaggtcc tcgtggccct tggcgaagcc ggcgatgttg gtggactcgg ccaggtccgc
      301 cgagcacacg atgaacagcg gtctgtcgta gtcggccttg gccgtggcgt tgacccacgc
      361 gccccacgag gcgaacgccg cgcggttggc gaccttctcg ccgggcgcct tccagaagct
      421 gtcggggtag gccgagacgt cggtgaagcg cgggtcgtcg aagatctccg ccccgcggcc
      481 gccgaggttg aaccccttta cccgctcggg cacgaggccg gcaacctcga ccagccggtc
      541 gctgatcgcg tccacgacct cggtgtggcg ccgaaggacg ccgatcgcga cctcgaggtt
      601 gtggcgggac tgcgcggtcc gctcggcggc atccgccggg gccggttcgt ccaccccggc
      661 gtaggtcacc ccatgccgct ccatgaactc gtggcgaacg gcccagaact cgggtgcgtt
      721 catgggccac ggcgtgccgt gggacttgtt gtcgtacttc ccgtagccgc gacccttccg
      781 ggttcggaac cacgcggccg agggcacgcg ctcggggttc tcgccgcggg cggcctccag
      841 cacggcggcg gtcaccggtc cccactcgga gccctccatc gtgccgttca cgcgccagtg
      901 gtaggcctcg aaccagctct gcggggtgcc gggtacgacc gacgagatcg ccgggtcgtc
      961 gatcccgtag tcattccagt cgagcaggaa gacgaggttc gacaggccca ggccccaggc
     1021 cgaattccgg gtctcgtggc tggctcccgg ggtcaggccg ccttcgccct cgaccacgaa
     1081 gaccttgacc tcctcggcac cggccagctt cagcgcgacc gcctgaccca gcgatggcgg
     1141 catgccgtgg cccgacggcc cggtgttcca cttcaggaag agggtcttgc cctccatctc
     1201 ggcgtggccc gggaggccac cgcggcggcg gagaaggagg aggtcctccc agccgatcgc
     1261 ccacttcccg ccgtccggca gcgcgtaccg ctcgtccggg tcgcggtcgg cacgggcgcg
     1321 cagcgtctcg ttcaggaccg cgagggtggc atagatgagc ggcacggtat ggcccgccga
     1381 caggacgaag cggtccgcga acggcagcca cggccggcgg atgtcccagc gcatggcgcc
     1441 cgacagcatg agcgatacga gcaggtggac cttcgaacgg gacccgcccg gatggccgct
     1501 ctggcggtgg ttgagggcga ggtcgatcac ctcgtcgacg acgtcgcgga cgagctccca
     1561 gtgcgggtac tgggcctccg cctcggcgag gagccgatcg gccgggacgg gctggacggc
     1621 ggtcatccgg cggcggtcac cagctcgccg ctccgctcca ggcgcgccag cgaatcggtg
     1681 atccgctcga gcgcggcgct gaggttctcg cggctgttcg cgtaactgaa gcgcagatat
     1741 ccctcgccat gacggccgaa ggcggtcccc gccaggccgc agaccccgcc gtcgtacagg
     1801 aggtgggtcg cgatctcggt actggaccgg ccgaaggagc tgacgttggg gaacacgtag
     1861 aacgcgccgt gcggcatcac gcagctgacg ccggggatcg cgttgaggcc atccacgatc
     1921 agctggcgcc gggcgatgaa ctcggtccgc atcgcgtcgg ccgcgtcctg cgggccggtg
     1981 agggccgcga tggcgccggc ctgggcgaac gcgttcgtgc agctcaccga gttgatcacg
     2041 agccggctgt aggtgggcgc catccaggtc gggatgatcc cgtaccccag ccgccagccg
     2101 gtcatggcga acgtcttcga gaacccatcg aggacgatcg tgcgctcgag catgccaggc
     2161 tgcgtcacga tcgacggcgc ctcgacgtca taggtgatcc gcgagtaaac ctcgtcggtg
     2221 agtacccagg caggtgttcg ccct
//
LOCUS       DASYGU010000277         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_765612, whole genome shotgun sequence.
ACCESSION   DASYGU010000277 DASYGU010000000
VERSION     DASYGU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765612"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VGO32_07975"
     CDS             complement(<1..110)
                     /locus_tag="VGO32_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEV7200722.1"
                     /translation="MVSKILIANRGEIAVRILRTCREMGIPAVVAFSEPDR"
     gene            complement(110..580)
                     /locus_tag="VGO32_07980"
     CDS             complement(110..580)
                     /locus_tag="VGO32_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV7200723.1"
                     /translation="MSDVAEQAQDVAREAAALVPGLTERLNRHGLGEIEVRRGDLRIR
                     VVADGAAGSGSATAGVDVTVSRDGVGVSDAQLASAPLDPITSPAVGYFLYADGLGPGL
                     PVTAGTEIGFVEMLGIRHEVRATADGVVRHLVAESGEPVEYGQLLVELDRAGGG"
     gene            complement(577..1542)
                     /locus_tag="VGO32_07985"
     CDS             complement(577..1542)
                     /locus_tag="VGO32_07985"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEV7200724.1"
                     /translation="MTIETSPPEVSREAADAAWRRVQLARHPQRPRTLSLVAQIFEDF
                     VELHGDRTYRDDPAIVGGPARLDGRPVMVIGHQKGTDTESNIFRNFGSPMPEGFRKAQ
                     RLMRLADRIGIPVVTFLDTAGAFPGPAAEERGQAEAIASSIGLMTGLRVPIVVVIVGE
                     GGSGGALAIGVGDEVLALENAVYSVISPEGCAAILWRSPEAAPLAAAAMKMTAAEQQE
                     LGIVDGVIAEPADGAHTDLPATAAAIRHAILGALARLDRLDPDELRARRYARLRAVGT
                     VIEGEAAALRVDAPSFGERLGRLLGLPGSGGPVPTDEEDMDEEGA"
     gene            complement(1548..2492)
                     /gene="accD"
                     /locus_tag="VGO32_07990"
     CDS             complement(1548..2492)
                     /gene="accD"
                     /locus_tag="VGO32_07990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEV7200725.1"
                     /translation="MKIKFPFSRRTGPADAWTKCPRCDGQLFNRQLERNDRVCPTCDY
                     HFRLSAPERIALLLDPGSFEERDPGLVSGDPLGFIDSKPYPDRLESSRARSGLPEAAI
                     WGMGKIEGQPVALGLFDFRFMGGSMGSVVGEKVARCFEAALAEQVPAIVVSASGGARM
                     QEGTISLLQLAKTTAALGRLNEGGVPFISILTDPTTGGVLASFASLGDVILAEPGALI
                     GFAGARVASGTVGEELPEGFQSAERLMDHGFIDAIVTRGQLRATVARLLRVLPVAGQV
                     PVDNRRAMRPIGALQGLAKRVGGAVSETAGIDTEATSR"
     gene            complement(2489..2824)
                     /locus_tag="VGO32_07995"
     CDS             complement(2489..2824)
                     /locus_tag="VGO32_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200726.1"
                     /translation="MTLDATRRPTDAERIDQAVAALLDGTSAAVPPALAAELATAHAV
                     RAGLPLIPAGARFEAQLARQLGAVPDRRLAGFVRQHQRLVLTGAVGSVVVSTASVAVV
                     AWRVVHRTP"
     gene            complement(2857..3465)
                     /locus_tag="VGO32_08000"
     CDS             complement(2857..3465)
                     /locus_tag="VGO32_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV7200727.1"
                     /translation="MSEPRRRPEPTITPEPRQAPGGDPDLADVRAAQQDRVAFDRLYK
                     RYLDRVYSYAFYQLGDHHEAEDATERTFLAALAAIDGFTDEGATFRAWLFRIAHNAIA
                     NAHRSRRRRRTEPISDVEHHHAPGADPAGVVARADEGRELRAALARIPADRQQVIVLR
                     FVDGLSSREIGAVLDRSEGAARVLLHRALRDLAHELEKDADL"
BASE COUNT          528 a         1309 c         1253 g          490 t
ORIGIN      
        1 cgatccggct cgctgaaggc gacgaccgcc ggaatgccca tctcgcgaca ggtccgcagg
       61 atgcgaacgg cgatctcgcc ccggttggcg atcaggatct tggagaccat caaccgcccc
      121 cggcgcgatc aagctcgacc aggagctggc cgtactcgac cggctccccg ctctcggcca
      181 ccaggtggcg gaccacgcca tctgccgtgg cccggacctc gtgccgaatg ccgagcatct
      241 ccacgaaacc gatctcggtg ccggccgtca cgggcaggcc cgggcccaga ccgtcggcgt
      301 acaggaagta gcccaccgcg ggcgaggtga tgggatccag cggggcggag gcgagctggg
      361 cgtcgctcac gccgacgcca tcccgcgaga cggtcacgtc aaccccggcg gtcgccgatc
      421 cgctgcccgc cgccccatcg gccacgaccc ggatccgaag atccccgcgc cggacctcga
      481 tctcgcccag cccgtgccga ttcagccgct cggtcaggcc cggaacgagc gcggcggcct
      541 cccgggccac gtcctgcgcc tgttcggcca cgtcgctcat gccccctcct cgtccatgtc
      601 ttcctcgtcg gtcggcaccg gcccgccgct gcccggaagg ccgagcaacc gtccgaggcg
      661 ctcgccgaac gacggggcgt ccacccgcag cgccgcagcc tcgccttcga tgacggtgcc
      721 gaccgcccgg agccgagcat agcggcgagc gcgcagctcg tccggatcga ggcgatccag
      781 gcgtgcgagc gcgccgagaa tggcgtggcg gatggccgcc gcggtcgcgg gcaggtccgt
      841 gtgcgcgccg tctgccggct cggcgatgac gccgtccacg atcccaagct cctgctgctc
      901 ggccgcggtc atcttcatcg cggctgccgc cagcggggcg gcctccggcg agcgccacag
      961 gatggcggca catccctccg gcgagatgac cgagtacacc gcgttctcga gggccaggac
     1021 ctcgtcgccg accccgatcg ccagagcgcc acccgatccc ccctcgccca cgatgacgac
     1081 cacgatcggc acccggagcc cggtcatgag cccgatcgag ctcgcgatcg cctcggcctg
     1141 gccccgctcc tcggcggcag gcccgggaaa ggcgcccgcg gtgtcgagga aggtgacgac
     1201 cggaataccg atccggtcgg ccaaccgcat gaggcgctgt gctttgcgga agccctctgg
     1261 catcggcgag ccgaagttgc ggaagatgtt ggattcggtg tcggtcccct tctggtgacc
     1321 gatgaccatc acgggccgac cgtcgagccg cgccggccca ccgacgatgg ccgggtcatc
     1381 gcggtaggtg cggtcgccgt ggagctccac gaagtcctcg aagatctggg ccacgagcga
     1441 aagcgtgcgc ggtcgttgcg ggtggcgcgc gagctggacc cgccgccagg cggcatcggc
     1501 ggcctctcgg ctgacctcgg gcggactggt ttcgatggtc atgtcggtca tcgggaggtg
     1561 gcctcggtgt cgatcccggc cgtctcgctc accgcgcccc cgacgcgctt ggccaggccc
     1621 tggagagccc cgatcgggcg catcgcgcgg cgattgtcga ccggcacctg gccggcgacg
     1681 ggcaggacgc ggaggagcct ggcaacggtc gcccgcagct ggccacgcgt cacgatggcg
     1741 tcgatgaagc cgtggtccat cagccgttcg gcgctttgga aaccctcggg cagctcctcg
     1801 ccaaccgtcc cgctggccac ccgggccccg gcgaagccga tgagcgcacc gggctcggcg
     1861 agaatgacgt cgcccagcga cgcgaagctg gcgagcacgc ccccggtggt gggatcggtc
     1921 aggatgctga tgaacgggac gccgccctcg ttcaggcgcc ccagggccgc ggtcgtcttg
     1981 gccagctgaa ggagcgagat cgtgccctcc tgcatgcgtg ccccgccaga cgcgctcacg
     2041 acgatggccg ggacctgctc ggccagggca gcctcgaagc aacgggctac tttctcgccc
     2101 acgacactcc ccatcgagcc gcccatgaac cggaagtcga acaggccgag cgccaccggc
     2161 tgaccctcga tcttgcccat cccccagatc gcggcttcgg gaaggccgga gcgcgcccgc
     2221 gaggactcca gccgatcggg atacggcttg ctgtcgatga acccgagagg atccccgctc
     2281 accaggcccg gatcgcgctc ctcgaacgag cccggatcga gcaggagtgc gatccgctca
     2341 ggagcgctca gacggaagtg gtagtcgcac gtcgggcaga cgcggtcgtt ccgctcgagc
     2401 tgccggttga acagctggcc gtcgcagcgc gggcacttgg tccatgcgtc ggccggcccg
     2461 gtccggcgac tgaacgggaa cttgatcttc atggcgtccg atgcaccacg cgccaggcca
     2521 cgaccgcgac gctcgccgtg ctgaccacga ccgatccgac cgcgccggtc agcaccagcc
     2581 gttggtgctg acggacgaac ccggccagcc gtcggtccgg caccgcgccg agctgacggg
     2641 ccagctgggc ttcgaatcgg gcgccggccg ggatcagggg cagcccggcc cggacggcat
     2701 gcgcggtcgc cagctcggcc gcgagggcgg gcgggacggc ggccgacgtg ccgtcgagga
     2761 gcgcggccac ggcctggtca atccgttcgg cgtccgtggg acggcgagtc gcgtcgagag
     2821 tcatctacag cggctaacac cgccggcccg gattcgttac agatcggcgt ccttctcgag
     2881 ctcgtgggcg aggtctcgga gcgcgcggtg aaggagcacg cgggccgcgc cctcgctgcg
     2941 atccaggacg gcaccgatct cccgcgacga gagcccatcc acgaagcgga gcacgatgac
     3001 ctgctgccga tcggccggaa tccgagcgag cgcggcgcga agctcacggc cctcgtcggc
     3061 acgggccacg acgccggccg gatcggcccc cggagcgtgg tgatgctcga catcggagat
     3121 cggctctgtc cggcgccggc gacgcgagcg atgggcattc gcgatcgcgt tgtgcgcgat
     3181 ccggaacagc caggcgcgga aggtggcccc ctcatcggtg aacccgtcga tcgccgccag
     3241 ggcggcaagg aacgttcgct cggtggcgtc ctccgcctcg tggtggtcgc cgagctggta
     3301 gaaggcgtag ctgtataccc ggtccagata gcgcttgtag agccgatcaa acgcgacccg
     3361 gtcctgctgt gccgcgcgca cgtcggcgag gtccggatcg ccgcccggcg cctgacgggg
     3421 ttcgggcgtg atcgttggtt cggggcgccg tcgcggctcg ctcatcggga tcctgccgcc
     3481 gcgtgaacgc ggatgacctc gcccacgttc accgtggtct ggatggtcgg cccgtccagc
     3541 accaggccgc cgttttcggc aatcgccccg gccacgccgg
//
LOCUS       DASYGU010000278         6660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1485552, whole genome shotgun sequence.
ACCESSION   DASYGU010000278 DASYGU010000000
VERSION     DASYGU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485552"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VGO32_08005"
     CDS             <1..1432
                     /locus_tag="VGO32_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7200728.1"
                     /translation="TRVRALALPGIGLVPESRRVYTMAGAAPGTSLAAQVLGFVNVDG
                     AGQYGVEGAEDAVLAGTPGSVIAQEDVAGRLIAGSVHELEAPINGADLTLTIDAGLQH
                     LLEEQMLDTFTRNRAKGVTGVIMDVNTGAILALATAPTFDANSYPSTDPALFSSPAVS
                     RQYEPGSVLKAMTVAAALDAGAITPTDLFLDDNHLQVYDATISNADRQWWPNGHGLIT
                     PAQVLALSNNVGAATIGLTLGGQRLYDALLRFGFGTPTGIDLAGEAAGTVLHPDAEGA
                     SKELTTAQNAFGQGISVTVVQMAAAYAAIANGGRLVSPHVVAGWTAEDGTFSPRPADE
                     QAQILKPETASTVLEMLTGAVDNGIADGAAVAGYSIAGKTGTAQVAGPVNVRVQTGWD
                     ANGKPIYVDSVQQAYIEGWVDSSFIGIAPASNPRFVMMVLIHRPVVGRSGIGERPEVA
                     FSQLAPLVFDYLGIPPDRPAPGVAQP"
     gene            1471..2874
                     /gene="murF"
                     /locus_tag="VGO32_08010"
     CDS             1471..2874
                     /gene="murF"
                     /locus_tag="VGO32_08010"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV7200729.1"
                     /translation="MPPIRIDDLLAATGGRLQAPTTVRSFRLAVVDSRRVTPDSLFVA
                     LPGERTDGHRFVAEAIAAGAAAVLVEREVDLPAGTDAAVVRVTDSLRAFQDLAAWWRE
                     RFPVRVVGVTGSTGKTLAKEVIADVLGRTLGVLRNEGNQNSETGLPMTLLRLEPAHQV
                     AVLEMGMYTVGEIARLVEISRPEVGVVLAVHPTHLERTGSLERIAEAKSELPRGLPAD
                     GLAVLNADDPRVAAMAAVSPARVRTFGLAENADIRATDIESEGLSGMRFTMHGPWGER
                     RVRSATPGRHLVPHALAAAAVADHFAVPLGEVAAALEAGSGAPHRMAIEALPSGATLV
                     DDTYNASPVSVSAALAFLAETPVGNGRRLAVLGDMLELGPDEQALHRRIGAEAATVLD
                     GLIAVGARGQWIADAARSAGMDRVATASDADSALEVVDRVLSPSAGDVLLVKGSRGVA
                     LDRLVAALTGRPPADGS"
     gene            2877..3857
                     /gene="mraY"
                     /locus_tag="VGO32_08015"
     CDS             2877..3857
                     /gene="mraY"
                     /locus_tag="VGO32_08015"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV7200730.1"
                     /translation="MLPLLLGLLLAFGGVVLLGPIYIRLLQRLGFGKAIRAEGPPTHA
                     VKAGTPTLGGMLIIVVVMFLAMALRIEDESTLIPMLTLVGVGILGAIDDYVNTRTGFG
                     VRGRYKLVWQTIVSVLAAIYIQRHFDITAINVPLVGELAIGAVAVIVFVAFAIVATSN
                     AVNLTDGLDGLASGILVFSFVAYLLIALMEVTGTKAAQPELAVFCALIVGALMGFLWF
                     NVHPAQIFMGDAGALSLGATLAVVATVSGQLLLLVIIGIVFVAVTMSDILQFASYRIR
                     GKRIFRMAPLQHHFELLGWAETKITLRFWIVSALAALLGFSLFLASTGLS"
     gene            3863..5293
                     /gene="murD"
                     /locus_tag="VGO32_08020"
     CDS             3863..5293
                     /gene="murD"
                     /locus_tag="VGO32_08020"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV7200731.1"
                     /translation="MSPSSARTIPSSRSELAGRRVLVLGLARSGVAAARFLVDAGAVV
                     TVYDKRSASELSDAVESLGERPVDLALGAAPERVRDLLGKADLVISSPSISARFPTTD
                     PWLRQALSDAEASGTPLVSEVDLFLRLTRARILAVTGTKGKTTTATIAAGILAAGKVP
                     HVLGGNIGTPLIEEADRLGRSTWAVLELSELQLPTISRGADIAVYTNILADHLDRHGS
                     VEAYRAVKRRLAELSAADGALVLSRDDPGSEELAASLPEARIHWYGLEARPGLDAWVE
                     GGWVVVGGERVLPRGDVPVRGDHLLRDVLAAAVATRLAGADTTAVATGVRAFGGVPHR
                     LESIGTRGGVEYVNDSQATIPVAAIAALDAFEERGLVVIAGGQGKGLDYGDFIDALAV
                     RCRSAVLIGETAAELERLLDGRVPVIRSGSMPEAVAAAAAAARPGDVVLLAPAAASFD
                     MFVDYAARGDAFRAAVEALPAAEGRS"
     gene            5290..6555
                     /gene="ftsW"
                     /locus_tag="VGO32_08025"
     CDS             5290..6555
                     /gene="ftsW"
                     /locus_tag="VGO32_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEV7200732.1"
                     /translation="MTAAVGAAGTTRRATRTRGVQRERKRVSMPLLVAVMSLVAIGVV
                     MVYSASSINALLNSDDPARYGIAQAVFAAIGLVGMVIVSRIDFRIYRYFALPALFVSW
                     ILLVIVLVPSIGHEAGGSRRWLDIPIVGGFQPAEVAKLAIVLYLAHWLDRRGRAAHGF
                     RGGLIPFALLVAPGFVLIAMEPDLGTAGIYVVAAASVFFMSGASVTGMLAVVGAIAAA
                     GFVVVSRNPYQLQRITSFLDPEKDPLGTGYNAMQALMALAMGGIAGVGLGASRQKYMY
                     LPAPSTDFIYAIIGEEWGLIGTVVVLLLFLAVAWQGYRIAVHAPDTFSGLLAAGITTW
                     LVAQAAINMMVVTALWPVTGIPLPFISYGGTALIINLVAVGVLLSISREAQTGSVFDA
                     LGDFRRRDRRAHLPRPGRRGRPVRAATRG"
     gene            6461..>6660
                     /locus_tag="VGO32_08030"
     CDS             6461..>6660
                     /locus_tag="VGO32_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200733.1"
                     /translation="MRLVISGGGTGGHIYPALAVADALCELQPGVELAYVGGVRGFER
                     RLVGEAGDMPYHQLTVRSLRSAG"
BASE COUNT          870 a         2375 c         2356 g         1059 t
ORIGIN      
        1 tacccgcgtg cgcgcgctcg cgttgccggg gattggcctg gttcccgagt cgcggcgggt
       61 ctacaccatg gccggcgccg cgccgggaac gagcctggcg gcgcaggtcc tgggcttcgt
      121 gaacgtcgac ggcgcgggcc agtacggggt ggagggggcc gaagacgcgg tcctggccgg
      181 caccccggga tcggtgatcg cccaggagga cgtcgccggc cggctgatcg cggggtcggt
      241 ccacgagctc gaggcgccta tcaacggtgc cgacctgacg ctcaccatcg acgccgggct
      301 ccagcacctc ctcgaggagc agatgctcga caccttcacc cggaaccggg cgaaaggggt
      361 caccggcgtg atcatggacg tgaacaccgg cgcgatcctg gctctcgcca cggcgccgac
      421 cttcgacgcc aacagctacc cgagcaccga tcccgccctc ttctcgagcc cggccgtcag
      481 ccggcagtac gagccaggat cggtgctgaa agccatgacc gtggccgccg cgctcgacgc
      541 gggcgcgatc accccgaccg acctgttcct cgacgacaac cacctccagg tctacgacgc
      601 gacgatcagc aacgccgacc gccagtggtg gccgaacggc cacgggctca tcacgccggc
      661 ccaggtgctc gccctctcga acaatgtcgg cgcggcgacc atcggcctga ccctgggcgg
      721 ccagcgcctg tacgacgcac tcctgcgctt cgggttcggg acgccgaccg gcatcgacct
      781 ggccggcgag gcggccggca ccgtgctcca tcccgatgcc gaaggcgcct cgaaggagct
      841 caccaccgca cagaacgcgt tcggccaggg gatcagcgtg accgtcgtcc agatggcggc
      901 cgcgtatgcc gcgatcgcga atggcggacg gctggtgagc ccgcacgtgg tggccggctg
      961 gacggccgag gacggcacgt tctcgccgcg gcctgctgac gagcaggccc agattctcaa
     1021 gcccgagacc gcctcgaccg tgctcgagat gctgaccggg gcggtcgata acggcatcgc
     1081 ggatggcgcg gccgtggccg gctattcgat cgcaggcaag accggcaccg cccaggtcgc
     1141 gggtccggtg aatgtgcggg tccagaccgg gtgggacgcg aacgggaagc cgatctacgt
     1201 cgactcggtc cagcaggcgt atatcgaggg ctgggtggac tccagcttca tcgggatcgc
     1261 cccggcgtcc aacccccggt tcgtgatgat ggtcctcatc caccggccgg tcgtgggccg
     1321 gtcggggatc ggggagcggc ccgaagtcgc cttctcccag ctcgccccgc tcgtattcga
     1381 ctacctcggc atcccgcccg accggcccgc gccgggggtt gcccagccat gaggctggta
     1441 gactcgccgc cgaacgccgc agggaaccgc gtgccgccga tccgcatcga cgacctgctg
     1501 gccgccaccg gcggccggct ccaggctccg accaccgtcc ggtccttccg ccttgccgtc
     1561 gtcgactccc gccgcgtcac gcccgatagc ctgttcgtgg cccttcccgg tgagcgcacc
     1621 gatggccacc ggttcgtggc cgaggccatt gccgcggggg ccgcggcggt gctcgtggag
     1681 cgcgaggtgg acctgccggc cgggaccgat gccgcggtgg tccgggtcac cgacagcctc
     1741 cgggccttcc aggacctggc cgcctggtgg cgtgagcggt tcccggtccg ggtggtgggc
     1801 gtcaccgggt cgactggcaa gacgctggcc aaggaggtca tcgccgacgt cctgggtcgc
     1861 accctcggcg tcctgcgcaa cgagggcaac cagaactccg agaccggtct ccccatgacg
     1921 ctgctccgcc tcgagcctgc ccaccaggtc gcggtcctcg agatggggat gtacaccgtg
     1981 ggcgagatcg cccgactcgt cgagatcagc cgtcccgagg tgggcgtcgt gctcgccgtc
     2041 caccccaccc acctcgagcg aacgggcagc ctggagcgga tcgccgaggc caagtccgag
     2101 ctcccgcgag ggctgccggc cgatgggctg gcagtcctga acgccgatga cccgcgggtc
     2161 gcggccatgg ccgcggtcag cccggcccgg gtccgcacgt ttggcctggc cgagaacgcc
     2221 gacatccgtg ccaccgacat tgagtccgag ggcctgtccg ggatgcgctt cacgatgcat
     2281 ggcccctggg gggagcgccg tgtgcggagc gcgacgccgg gtcgtcatct cgtgccgcat
     2341 gccctggccg cggccgcggt ggcggatcac tttgccgtgc cgctgggtga ggtggccgcc
     2401 gcactggagg ccggcagcgg cgccccgcac cggatggcca tcgaggcgct gcccagcggg
     2461 gcgaccctgg tcgatgacac gtacaacgcg tcgccggtct ccgtgtcggc ggcactggcg
     2521 ttcctggccg agacgcccgt tggcaacggt cggcggctgg ccgtcctggg cgacatgctc
     2581 gagctcgggc ccgacgagca ggcactccat cggcgaatcg gcgccgaagc ggcgaccgtg
     2641 ctcgatgggc tgatcgcggt gggggcgcgc ggccagtgga tcgccgacgc cgcgcgatca
     2701 gcgggcatgg accgggtggc caccgcttcc gacgccgaca gcgcgctcga ggtcgtggat
     2761 cgcgtgctga gcccgtcggc cggcgacgtg ctcctggtca agggttcgcg cggggtggcc
     2821 ctcgaccgac tggtcgccgc gctgaccggc cggcccccgg cggacggctc ctgaccgtgc
     2881 tgcccctcct cctcgggctg ctcctcgcct ttggtggcgt cgtgctgctt ggcccgatct
     2941 acatccgcct gctccagcgg ctgggcttcg gcaaggcaat tcgggccgag ggcccgccga
     3001 cccacgcggt gaaggccggc acgccgaccc tgggcgggat gctgatcatc gtggtcgtca
     3061 tgttcctggc catggcgctg cggatcgagg acgagagcac cctgatcccg atgctgaccc
     3121 tggtcggagt gggcatcctg ggggcgatcg acgactacgt gaacacgcgg accgggttcg
     3181 gcgtgcgcgg gcgctacaag ctggtgtggc agacgatcgt gtcggtcctg gccgcgatct
     3241 acatccagcg ccacttcgac atcaccgcca tcaacgtccc gctcgtcggc gagctggcga
     3301 tcggcgcggt cgccgtcatc gtgttcgtgg ccttcgccat cgtggccaca tccaacgcgg
     3361 tgaacctcac cgatggactg gacgggctgg cctcgggcat cctggtcttc agcttcgtgg
     3421 cgtacctcct catcgcgctc atggaggtga ccggaaccaa ggccgcccag cccgagctcg
     3481 ccgtgttctg cgcgctgatc gtcggcgcgc tgatgggctt cctgtggttc aacgtccacc
     3541 ccgcccagat cttcatgggc gacgccgggg ctctgtcgct cggcgccacg ctggcggtgg
     3601 tggccaccgt cagcggacag ctgctcctgc tcgtgatcat cggcatcgtg ttcgtggccg
     3661 tgaccatgtc ggacatcctc cagttcgcct cgtacaggat ccgcgggaag cggatcttcc
     3721 ggatggcccc gctccaacac cactttgagc tgctgggctg ggcggagacg aagatcaccc
     3781 tccgcttctg gatcgtgtcg gccctcgccg cgttgctggg cttcagcctg ttccttgcct
     3841 cgaccggtct gtcgtgatgc cgatgtcgcc ctcgagcgcc cgcacgatcc ccagcagccg
     3901 gtccgagctg gccgggcgtc gcgtcctggt gctgggcctg gcccgctcgg gcgtcgcggc
     3961 ggcccgcttc ctggtcgacg cgggggcggt cgtgaccgtc tacgacaagc ggtcggccag
     4021 cgagctgtcc gatgccgtcg aatccctcgg cgagcggccg gtggacctgg ccctcggggc
     4081 cgcgcccgag cgcgtgcgcg acctcctcgg gaaggccgac ctcgtgatca gcagcccgtc
     4141 gatctccgca cgcttcccga cgaccgatcc atggctccga caggcgctgt ccgacgcgga
     4201 ggcgtccggc acgcccctcg tcagcgaggt cgacctgttc ctgcgtctga cccgggcgcg
     4261 gatcctggcc gtgaccggca ccaagggcaa gacaaccacc gcaaccatcg ccgccggaat
     4321 cctggccgcc gggaaggtgc cgcacgtgtt gggcgggaat atcgggacgc ccctcatcga
     4381 ggaggccgat cgactcggac ggtccacctg ggcggtcctc gagctgtcgg aactccagct
     4441 gccgaccatc agtcggggag cggacatcgc ggtctatacc aacatcctgg ccgaccatct
     4501 cgaccgccac gggagcgtcg aggcctaccg ggccgtgaaa cgcaggctgg ccgagctgtc
     4561 agcggccgac ggggcgctgg tcctgagccg ggacgaccct gggtcggagg agctggccgc
     4621 gagcctgccc gaggcccgca tccactggta cgggctggaa gcgcgccccg gtctcgacgc
     4681 gtgggtcgag ggcggctggg tggtggtcgg cggcgagcgc gtcttgccgc gcggcgacgt
     4741 acccgtccgt ggtgaccacc tgctccgcga cgtgctggcc gccgcggtcg cgacccgtct
     4801 tgccggggcg gatacgacgg ccgttgcgac cggcgtgcgc gcgttcggcg gtgtgccgca
     4861 tcggctcgag tcgatcggga cccggggcgg ggtcgagtac gtgaacgact cgcaggccac
     4921 gatcccggtc gccgcaatcg cggccctcga tgcgttcgag gagcggggtc tggtggtcat
     4981 cgcgggcggc cagggcaagg gcctcgacta cggcgacttc atcgatgcgc tggccgtccg
     5041 gtgccgatcg gcggtgctca tcggcgagac ggccgcggag ctcgagcggc tgctcgacgg
     5101 acgggtcccc gtcatccgca gcgggtcgat gccggaggcg gtcgccgccg ccgccgcggc
     5161 cgcgcgcccg ggcgacgtcg tcctgctggc tcctgccgcc gcgagtttcg acatgttcgt
     5221 cgactacgcc gcccgcggag atgccttccg agccgccgtc gaggccctgc cggccgcgga
     5281 gggacgctca tgaccgcggc cgtcggcgcg gccggcacca cccgtcgggc gacgcgcacc
     5341 cgcggcgtcc agcgcgagcg caagcgggtg tcgatgccgc tcctggtggc ggtcatgtcc
     5401 ctggtcgcga tcggggtcgt gatggtgtac tcggcatcga gcatcaacgc cctgctgaac
     5461 agcgacgacc cggcccgcta tggcatcgcg caggccgtgt tcgccgcgat cggcctggtg
     5521 ggcatggtca tcgtcagccg gatcgacttc cgcatctatc gatacttcgc cctgcccgcc
     5581 ctgttcgtct cgtggatcct gctcgtcatc gtgctcgtgc cgtccatcgg tcacgaggca
     5641 ggaggctcgc gccgctggct cgacatcccg atcgtcggcg gcttccagcc ggccgaggtc
     5701 gcgaagctgg ccatcgtcct gtacctcgcc cactggctcg accggcgggg ccgcgcggcc
     5761 cacgggttcc gcggtggcct catcccgttc gcgctgctgg tcgcccccgg gttcgtgctc
     5821 atcgcgatgg aaccggacct cggcacggcg gggatctacg tcgtcgcggc ggcgtcggtc
     5881 ttcttcatgt ccggcgccag cgtgaccggg atgctcgccg tcgtcggtgc gattgccgcg
     5941 gcaggtttcg tggtcgtcag ccgcaacccc taccagctgc agcggatcac gagcttcctc
     6001 gaccccgaga aggacccgct cgggaccggc tacaacgcga tgcaggcgct catggcgctg
     6061 gccatgggcg ggatcgccgg cgtgggcctg ggtgctagcc ggcagaagta catgtacctg
     6121 ccggccccgt cgaccgactt catttacgcc atcatcggtg aggaatgggg cctcatcggg
     6181 acggtggtcg tcctcctcct gttcctggcc gtggcctggc agggttaccg gatcgcggtt
     6241 cacgctcccg ataccttctc cggcctgctg gcggccggga tcacgacctg gctcgtcgcc
     6301 caggctgcca tcaacatgat ggtcgtgacc gcgctgtggc cggtcaccgg cattccgctg
     6361 ccgttcatca gctatggcgg gacggccctc atcatcaacc tcgtggcggt cggagtgttg
     6421 ctctcgatca gtcgcgaggc ccagacagga tcggtgttcg atgcgcttgg tgatttcagg
     6481 cggcgggacc ggcgggcaca tctaccccgc cctggccgtc gcggacgccc tgtgcgagct
     6541 gcaacccggg gttgagcttg cgtacgtggg tggtgtccgt ggcttcgagc gccggctggt
     6601 gggagaggcc ggcgacatgc cctaccacca gctgaccgtc cgctcactgc gctcggccgg
//
LOCUS       DASYGU010000279         9924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1177743, whole genome shotgun sequence.
ACCESSION   DASYGU010000279 DASYGU010000000
VERSION     DASYGU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177743"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGO32_08035"
     CDS             complement(<1..379)
                     /locus_tag="VGO32_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200734.1"
                     /translation="MPDTEAAAWPKGIDAITLFVEDLDAARRFYRDVFGLPVMYEDPD
                     SAGFNFGNTVINLLRVSAASSLIEPAAVASPDAGSRMQFTIEVDDVDAMCATLQQRGV
                     ELLNGPMDRPWGIRTASFSDPGGH"
     gene            complement(393..548)
                     /locus_tag="VGO32_08040"
     CDS             complement(393..548)
                     /locus_tag="VGO32_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200735.1"
                     /translation="MRFLAAVVLVVILLVGAFKLSGMQLPILDYPLGGPMSQPRIEID
                     PNPDLFP"
     gene            643..1068
                     /locus_tag="VGO32_08045"
     CDS             643..1068
                     /locus_tag="VGO32_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7200736.1"
                     /translation="MNDFRSTASITINAAPASVWEALTTPALMKQWFFGTDTETDWTV
                     GSPIVHRGEYQGQAYVDKGTVLAFEPERLLVHTHWSSVSGVPDLLENYQTVSWVLTER
                     DGQTDLTVTDDNIRSEEARVASDQGWETALSALKEVVES"
     gene            1065..1994
                     /locus_tag="VGO32_08050"
     CDS             1065..1994
                     /locus_tag="VGO32_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200737.1"
                     /translation="MTVAPASVIDWLLASDPAIRWQVMRDLTDAPTGEIASERAKVAT
                     EGWGAQLLALQGADGYWGGGAYKPDWTGTNGTLQLLRRFGVDPVVEPVRRALRLVREN
                     VLWEYDDLPYFEGEVEPCINGQAVAVGAYFGEDVQGIVDRLLTEQMADGGWNCEQENG
                     STRGSFDSTINVLEGFLEYERAVGGNQEVAAARVRGQEYLLERRLFHRLSTGEVTEPN
                     TGVWVRFAFPTGWHYDVMRGLDHLRDAAAIPDDRLADAIALVESKRDAEDRWPREITY
                     HDRLDVEMDEGEDLPSRWMTLRALRVLRWYHGS"
     gene            1991..3154
                     /locus_tag="VGO32_08055"
     CDS             1991..3154
                     /locus_tag="VGO32_08055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7200738.1"
                     /translation="MKPTPRRRVVQLLATGDLGGAQGSVTDLMLRLDPIRFAVEAVSL
                     TDGPAVDRMRELGLPAIVLDEADDQALVRRLAVHLRDRDGDLLHAHMFRAELLGARAA
                     RMAGTKAIVATVHSSRVRSEPEVSALAAENAFFDRLIAPSGAIAAKVHREGRGAVPIT
                     VVPNGVDLDRFGAHRSSDGRNELRATLGIPADAFLIGVVARLEPEKGHRYLLAAWPEI
                     AEALPDAWLVLVGDGSLGSVLRAQASSLPAPTDQHVRFAGGRTDVVAMTQALDLAVLP
                     SLREAQGIALLEAMAARVPVVASRVGGIPETIRDEVDGLLVPPADHDALAAAVIRLSR
                     DLVMRRDLSAAGRRRVEDRFNVIESVRRIEAIYLEELTRAAPLPDAAREAADG"
     gene            3147..3542
                     /locus_tag="VGO32_08060"
     CDS             3147..3542
                     /locus_tag="VGO32_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200739.1"
                     /translation="MADQPPPIRVGSIVIRVDDLERQKAFWMAALDYVPREGDDDTFA
                     LIRPRDGNGPNVSLDRWPAPLQVPPKIHLDLYAQDQAAEVQRLIGLGATEVDWPRMPA
                     DADFVILADPEGNRFCVIDVHWEPPTEDA"
     gene            3664..4518
                     /locus_tag="VGO32_08065"
     CDS             3664..4518
                     /locus_tag="VGO32_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200740.1"
                     /translation="MARSVLLAGALSVLITAPAWAADILIYGPADATESGVATTGGHT
                     VTIADATAWAAMTTGDFAAFDAIVIGDSGCTVGASPNLDVATSNRTTWASAVTGPIVA
                     LTWDPGAHGDGAGPNQTDELTLNAINYAASGTSTGLYFALGCYYLDNALTAVTLLDPF
                     GTFTVNDQSADTITILDPAHPTMTGLTEAGLSNWGSSTHAQFLAFPAGFQAIANSDNG
                     SLVLPVIVTRAAAPVAPTPTPGPLPDAAATQTSSGFLAPLLGLLLLVAAAVSIGLQVR
                     RRGDFRRD"
     gene            4675..5037
                     /locus_tag="VGO32_08070"
     CDS             4675..5037
                     /locus_tag="VGO32_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7200741.1"
                     /translation="MQAEQLDSVFNALADPTRRAILARLTEGDANVAELAAPFRMSQP
                     AVSRHLKVLEQAGLISRHRRATARLSHLEAEPLREATTWLADYREYWTESYERLDELL
                     ERLQRERSKRQSAKPKRS"
     gene            5040..5528
                     /locus_tag="VGO32_08075"
     CDS             5040..5528
                     /locus_tag="VGO32_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7200742.1"
                     /translation="MSTTTFNVPEGLPFVDITREFDAPVDLIHRAYTDPELVPQWLGP
                     KGYAMDLEKWEAWDGGSWRYVQTDPDGGKWGFHGVFHGAQTPDQMIQTFEFEGVPGHV
                     SFSELKLEDLGNGRSVARTHVIFMSLEDRDGMVQSGMEFGVVQGYERLDELVGTLAPV
                     AS"
     gene            5525..5971
                     /locus_tag="VGO32_08080"
     CDS             5525..5971
                     /locus_tag="VGO32_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7200743.1"
                     /translation="MTSPLEYRLEVVVVPVSDVDRAKAFYADQVGFHVDVDQKNSDLR
                     IVQMTPNGSACSVTIGIGVADMPPGAVKGLQLVVQDIDAARADLASRGVEVTPVRHHD
                     GTGWADGPGGDWNSFVFFNDPDGNAWTVQQSPGWKEAVADGAEDGA"
     gene            5961..6398
                     /locus_tag="VGO32_08085"
     CDS             5961..6398
                     /locus_tag="VGO32_08085"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV7200744.1"
                     /translation="MAPKPGGAAKVDAYLAALPAEQAEVLQDLRETILAVVPNPEEAI
                     SYAIPGVKYKGKGLVWYAAFKAHCSFFPGAKVEHYTAELDGYKIHKGTVQFTPDHPLP
                     ADLVQRMVRHGMTEIDARVEAKIATRAEARAEAEAAGKRKRRS"
     gene            complement(6395..7087)
                     /locus_tag="VGO32_08090"
     CDS             complement(6395..7087)
                     /locus_tag="VGO32_08090"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEV7200745.1"
                     /translation="MPWFRRRSTPRSVSPDEAREWMVQTQLENRGIRDPEVLAAMRGV
                     PRHQFVDSEYAYDDRALPIAGGQTISQPFVVALMTQLARPPRGWRRAHVLEVGTGSGY
                     QAAVLAELGASVVSVERDPKLSAQAAENLASAGYDQVLTEIGDGTRGWPAGAPYDAIL
                     VTAAGPSVPDPLREQLSPDGGRLVIPIGEREGQWLTLVERTGDDWTQQPMEPVVFVPL
                     VGAHGYEERRLH"
     gene            complement(7130..7399)
                     /locus_tag="VGO32_08095"
     CDS             complement(7130..7399)
                     /locus_tag="VGO32_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200746.1"
                     /translation="MFSRVKIAIRFFFVGLVVGVLLAPRSGRATRRLLRTRADRLFNE
                     LMDAATLGAYETVPGDIAEAEDADAGSSNGQPSTPRKKATAPAQS"
     gene            7527..7976
                     /locus_tag="VGO32_08100"
     CDS             7527..7976
                     /locus_tag="VGO32_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200747.1"
                     /translation="MHRPLALVAALALAACSPSASGPSADGAASASAVASQVAEAAGT
                     ITIVEGMAVDGPGLTIPEALNSGEADPLLVNGILLQDAEGTIWLCQSLSDSSPRACAG
                     AFLAVEDFPEGVGDLDPANADVTGLQEEDGVVWLEDHQLYGTVSPSN"
     gene            complement(7978..8253)
                     /locus_tag="VGO32_08105"
     CDS             complement(7978..8253)
                     /locus_tag="VGO32_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200748.1"
                     /translation="MSTLKPSDAGYRGALPPRNDWLARGWVVVVIAIFIGMFVLDVLN
                     VPTILFPPPSPVPSVGPAPSGSADASAEPSAGGSPSESGSADASSSP"
     gene            complement(8292..>9924)
                     /locus_tag="VGO32_08110"
     CDS             complement(8292..>9924)
                     /locus_tag="VGO32_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7200749.1"
                     /translation="KMPVFEGSLLAAGLTAADLVGDPYHVLLIANSTAEFVRQGGMFG
                     DVLPIPIPVLRDVVSVGDVLLWMGIVWAISAAMTRPVASRASAALGSSPARPMPAGEF
                     QLGVAYANAIALPAEPVPVLPGAGIGVGGEEEEERQSPYLRLATNRNYALLWSGQLVS
                     LMGDRINQVALGFLVATRGTALDVGFTMASIAIPNVIFGPWAGALADRWDRRRTMIAA
                     DLIRAALVLTIPLAFNVDIRLVYVVAFSVATVGLLFRPSKDALIPQIVEGEDLMAANS
                     ATSVTETLADLVGYPLAGLMVTALASIIGAAFVLDSGTYLISAALLALMHVARSERVA
                     SRLGFRQLWRDVREGISFLAHQAELRANTLVSTMAQVAVGTEVTCSLLYAQTVLDQST
                     IPFIENYALLMTAIGLGSIVGGLVIGGLLGGVRKGPMAIAGFIGMGLTFIVVGQVTDP
                     VIAIGLFFLIGVTNMAFVIPNITLFQERTPQALFARVVTSRQALVFGVMATAMAVSGI
                     VAGIIGAGETLMLGGAVAVAAGLVGLLIPSMRNAA"
BASE COUNT         1632 a         3405 c         3392 g         1495 t
ORIGIN      
        1 tgtggccgcc cgggtcgctg aaactggcgg ttcggatgcc ccacggtcga tccatcggcc
       61 cattcagcag ttcgacgcct cgctgctgga gcgtcgcaca catggcatcg acgtcatcga
      121 cctcgatggt gaattgcatc cgcgatccgg cgtcgggcga tgccaccgcc gccggctcga
      181 tgagggagct cgcggcgctg acccggagca gattgatgac tgtgttcccg aaattgaacc
      241 cggccgagtc ggggtcctcg tacatgactg gcagcccaaa gacatcgcga tagaaccgtc
      301 gcgccgcgtc aaggtcctcg acgaagagcg tgatcgcgtc gatacccttc ggccaagctg
      361 cggcctcagt gtctggcatc gttacctccg actcacggga acaagtcggg attcgggtcg
      421 atctcaatgc ggggctggct catcgggccg ccgagtgggt agtcaaggat cggcagctgc
      481 atgccggaca gcttgaaggc gccgaccagc agaatcacca cgagcacgac cgcggccaag
      541 aagcgcattg cgaggcagcc tactctcgac cgtctgccgg caacaggtgc ctttgggcta
      601 ccccgggtgt cctgccccga tccgtcctac catcgtgacc tgatgaacga cttccgcagc
      661 accgcttcga tcacgatcaa cgctgcaccg gcatcggtct gggaggcatt gaccaccccc
      721 gcgctcatga agcagtggtt cttcgggacc gacacggaga ccgattggac cgtcggcagc
      781 ccgatcgtgc accgcggcga gtaccagggc caggcgtacg tcgacaaggg cacggtcctt
      841 gcgttcgagc cggagaggct cctcgttcac acccactgga gctcggtgtc aggcgttccg
      901 gacctcctcg agaactacca gaccgtttcc tgggtcctga ccgagcggga cggccagacg
      961 gatctgaccg tcaccgatga caacatccgg agcgaggaag cccgcgtggc atcggaccag
     1021 ggttgggaga ccgcgttgtc cgcgctcaag gaggtcgtcg agtcgtgacg gtggcgccgg
     1081 cgagcgtcat cgactggctc ctcgcctctg atccagccat ccgctggcag gtgatgcggg
     1141 acctgaccga cgcgcccacc ggcgagattg cttccgagcg ggcgaaggtc gccacggagg
     1201 gctggggcgc ccagctgctg gcccttcagg gcgccgacgg ctactggggt gggggcgcgt
     1261 acaagcccga ctggaccggc accaacggaa cgctgcagct cctgcgccgg ttcggcgtcg
     1321 acccggtggt cgagccggtg cgccgcgcgc tccgtctcgt gcgggagaac gtgctgtggg
     1381 agtacgacga cctgccctac ttcgaaggcg aggtcgagcc ctgcatcaat ggccaggccg
     1441 tcgcggtcgg tgcctacttc ggcgaggacg tgcaggggat cgtggaccgg ctcctcaccg
     1501 agcagatggc cgatgggggc tggaactgcg agcaggagaa tggttccaca cgcggatcgt
     1561 tcgattccac gatcaacgtg ctcgaagggt tcctggagta cgagcgtgcc gtcggtggaa
     1621 accaggaggt ggccgcggca cgggttcggg gccaggagta cctcctcgag cgtcgactct
     1681 tccaccggct ctcgaccggc gaggtgaccg agcccaacac cggcgtctgg gtccgtttcg
     1741 cattcccgac cgggtggcac tacgacgtca tgcgcgggct ggaccacctc cgcgatgcag
     1801 ccgccatccc ggacgaccgc ctggcggacg ccatcgcact ggtcgagtcc aagcgagacg
     1861 ctgaggaccg gtggccgcgc gagatcacct accacgaccg actggatgtc gaaatggacg
     1921 agggcgagga cctccccagc cgatggatga cgctccgcgc cctccgcgtc ctgcgctggt
     1981 accacgggtc gtgaaaccga ccccgcggcg gcgagtggtc cagctgctgg ccaccgggga
     2041 cctcggcggc gcgcagggca gcgtcaccga tctcatgctc cgcctggacc ctatccggtt
     2101 cgccgtggag gccgtgtcgc tgaccgatgg ccccgcggtc gaccgaatgc gcgagctcgg
     2161 cctgccggcg atcgtcctcg acgaggccga tgatcaggct ctggtccgca ggctcgcggt
     2221 ccacctccgc gaccgcgacg gggacctcct ccacgcccac atgttccgcg ccgagctgct
     2281 cggagctcgc gcagcgcgga tggccggaac gaaggcgata gtggcaaccg tccactcgag
     2341 ccgggtgcgc tccgagcccg aggtttccgc cctcgccgcc gagaacgcat tcttcgatcg
     2401 actgatcgcc ccgtcggggg cgatcgcagc caaggtccac cgcgaaggcc gaggagcggt
     2461 gccgatcacc gtcgtcccca atggcgtgga cctcgatcgg ttcggcgccc atcggtcctc
     2521 cgatggtcgc aacgagttgc gagccacgct cggcattccg gccgatgcct tcctcatcgg
     2581 agtcgtggct cggctcgagc ccgagaaggg gcatcggtat ctccttgccg cctggccgga
     2641 gattgcggag gctctgcccg acgcctggct cgtgctggtg ggggatggct cgcttgggtc
     2701 ggtcctccgt gcgcaggcat cctccttgcc ggcgccgact gaccagcacg tgcgcttcgc
     2761 cgggggacgg accgatgtgg tggcgatgac gcaggccctc gatctcgcgg tcctcccctc
     2821 gctccgcgag gcgcagggca tcgcattgct ggaagcgatg gccgcacgcg tgccggtcgt
     2881 tgcctcgcgc gtgggcggca ttcccgagac gatccgcgat gaggtggatg gtctcctcgt
     2941 tccgccggcc gaccacgacg ccctggctgc ggccgtcatc cgactgtcac gagacctcgt
     3001 catgcggcgc gacctgtccg cggcaggacg gcggcgggtg gaggatcgat tcaacgtgat
     3061 cgaatcggtc cggcggattg aagcgatcta cctcgaggag ctgacacgcg cggctcctct
     3121 tccggatgcc gcacgcgagg ctgccgatgg ctgaccagcc gccgccgatc cgggtcggct
     3181 cgatcgtcat tcgggtagac gacctcgagc ggcagaaggc cttctggatg gccgccctgg
     3241 attacgtccc gcgtgagggc gacgacgaca cgttcgccct catccgaccg cgggacggca
     3301 acggcccgaa cgtgtcgctg gacaggtggc ccgccccgct ccaggttccg cccaagatcc
     3361 acctcgacct gtacgcccag gaccaggcgg cggaggtcca gcggctcatc ggcctcggcg
     3421 ccaccgaggt ggactggcca cggatgcctg ccgacgccga cttcgtcatc ctcgccgacc
     3481 cggaaggcaa ccgtttctgc gtgatcgacg tccactggga gccaccgacc gaggacgcct
     3541 gacccgctgg atcgtggtga acatctggtc gaaatggttg accgtggaag gccggaacgg
     3601 atagggttcc cggcgctggg gggaaccagg gggttcagga ggggcacaaa tttgggcact
     3661 cgtatggcac gaagcgtcct gctcgcaggc gcgctatccg ttcttatcac ggctcccgca
     3721 tgggcagccg atatcctgat ctacggaccg gccgacgcga ccgagagtgg cgtggccacc
     3781 accggtggac acacggtcac catcgcggat gcgacagcct gggcggccat gaccaccggc
     3841 gacttcgcgg cctttgacgc gatcgtgatc ggggactcgg gttgtaccgt cggtgcgtct
     3901 ccgaatctgg acgtggccac ctccaaccgg accacctggg cctccgcggt cactggtccg
     3961 atcgttgcgc tgacctggga tccgggcgcg catggcgacg gcgcgggacc gaaccagacc
     4021 gatgagctga cgctcaatgc catcaactat gcggccagcg gaacgagcac cggcctgtac
     4081 ttcgcgctcg gctgctatta cctggacaac gcgctcaccg cggtgacgct cctcgaccca
     4141 tttggcacct tcacggtgaa tgaccagtcg gccgacacga tcacgatcct ggaccctgcc
     4201 catccgacga tgaccgggct caccgaggct ggcctctcga actggggcag ctcgacgcac
     4261 gcccagttcc tcgcattccc ggcgggcttc caggccatcg ccaacagcga caacggatcc
     4321 ctggtcctcc ctgtgatcgt gacccgggca gccgcgccgg ttgcaccaac cccgacgccc
     4381 ggcccgctgc cggacgccgc cgcgacgcag acatcgtccg gattcctggc cccgttgctg
     4441 ggcctgctgc tgctggttgc agccgcggta tcgatcggtt tgcaagttcg tcgccgaggc
     4501 gacttccgcc gggactgagg ggggaccgtc tcagccacga ggccggactc acgtgctgag
     4561 cccggcaact cggcttttcc gaccacaatt cagtgccgcc cgcctgcatc gcttgacagc
     4621 cctcggcgga gggagtatct ataacccaca ccttctagaa caggacggtt atagatgcaa
     4681 gcggaacagc tcgactcggt cttcaatgcg ctggcggacc ccacccggcg ggccatcctg
     4741 gcccggctga cggaagggga tgccaacgtg gcggagctgg cggccccgtt ccgcatgtcc
     4801 cagccagccg tctcgcgtca cctgaaggtg ctcgagcagg ccggtctcat ctcccgacat
     4861 cggcgagcca ctgcccggtt gagccatctc gaggcggagc cgcttcgcga ggccacgacc
     4921 tggctcgccg actaccggga gtactggacc gagagctacg aacggctcga cgagctgctc
     4981 gagcgcctgc aacgcgagcg atccaagcga cagtctgcca agccaaaaag gagctgacca
     5041 tgtccaccac cacgttcaac gttcccgagg gcctgccctt cgtggacatc acccgcgagt
     5101 tcgacgcccc ggtcgacctc attcaccgcg cgtacaccga cccggagctc gtcccgcaat
     5161 ggctcggccc gaagggctat gccatggacc tcgagaagtg ggaggcctgg gacggcggct
     5221 cgtggcgcta cgtgcagacc gatcccgacg gcgggaaatg ggggttccac ggcgtcttcc
     5281 acggcgcgca gacgccggac cagatgatcc agacgttcga gttcgagggt gtccccggcc
     5341 acgtgtcgtt ctccgagctg aagctcgagg atctggggaa cggccgatcg gttgccagga
     5401 cgcacgtcat cttcatgtcg ctcgaggacc gcgacggcat ggtccaaagc ggaatggagt
     5461 tcggcgtggt ccagggatac gagcgcctcg acgagctcgt gggcaccctc gccccggtcg
     5521 cctcatgacc agcccgctgg agtacaggct cgaggtcgtg gtcgtgccgg tcagcgacgt
     5581 cgatcgggcc aaggccttct acgccgacca ggtggggttc catgtcgacg tcgaccagaa
     5641 gaacagtgac ctgcggatcg tccagatgac gcccaatggg tcggcctgct cggtgaccat
     5701 cgggatcggc gtggcggaca tgccgcccgg tgcggtgaag ggtctgcagc tggtcgtcca
     5761 ggacatcgac gcggctcggg ccgacctcgc ttcgcggggc gtggaggtca cgccggtccg
     5821 ccaccacgac ggaaccggct gggccgatgg gcccggcggt gactggaact cgttcgtctt
     5881 cttcaatgac cccgacggca atgcctggac cgtccagcag agcccgggct ggaaggaagc
     5941 cgttgccgat ggagccgagg atggcgccta aacccggtgg ggcggccaag gtggatgcct
     6001 acctggccgc tctccccgcc gaacaggccg aggtcctcca ggacctgcgc gagacgatcc
     6061 tggccgtcgt ccccaacccc gaggaggcga tcagctacgc catccccggc gtgaagtaca
     6121 agggcaaggg cctcgtctgg tacgcggcgt tcaaggccca ctgctcgttc ttcccgggcg
     6181 ccaaggtgga gcactacacg gccgagctgg acggttacaa gatccacaag ggcacggtcc
     6241 agttcacgcc cgaccaccca ctacccgctg acctcgtcca gcgcatggtt cgccatggga
     6301 tgaccgagat cgacgcgcgg gtcgaggcca agatcgccac ccgggccgag gcccgggcgg
     6361 aagcggaggc cgccggaaag cgaaaacgcc ggagctagtg gagtcgccgc tcctcgtatc
     6421 cgtgggcacc gaccagcgga acgaagacca ccggctccat cggctgctgg gtccagtcat
     6481 cgcccgtgcg ctcgaccagg gtcagccact ggccctcccg ttcgccgatc gggatcacga
     6541 gccgcccgcc atcgggtgac agctgctcgc gcagcgggtc ggggaccgat ggccccgcgg
     6601 cggtgaccag gatcgcgtcg tagggcgcgc ccgccggcca ccctcgcgtg ccgtcgccaa
     6661 tctcggtcag aacctggtca tacccggcgg acgcgaggtt ctcggccgcc tgcgcgctga
     6721 gtttcggatc acgctcgacg gagactacgg acgcgccgag ctcggccagg accgcggcct
     6781 ggtatccact cccggtccct acctccagca cgtgcgcgcg gcgccagccg cgcggcggac
     6841 gggcgagctg ggtcatgagc gcaaccacga acggctggga gatggtctgg ccgccggcaa
     6901 tgggcagggc gcgatcgtca taggcgtact ccgagtccac gaactggtgg cgcggcacgc
     6961 cgcgcatggc ggccagcacc tcgggatcac ggatcccgcg attctcgagc tgggtctgga
     7021 ccatccactc gcgggcctca tcgggcgaga ccgatcgcgg ggtgctccgc cggcggaacc
     7081 agggcatcgt gcgcggccat tctgcgccgc gcggaacgtg cgccggcggt caggactggg
     7141 cgggagctgt cgccttcttg cgcggcgtcg agggctggcc gttcgagctg cccgcgtcgg
     7201 cgtcctcggc ctcggcgatg tcgcccggta cggtctcgta tgcgcccagg gtcgccgcgt
     7261 ccatcagctc attgaagagg cggtcggcgc gggtgcggag caggcggcgc gtggcgcggc
     7321 cactgcgcgg ggcgagcaga acgcccacca ccaggcccac gaagaagaat cgaatcgcga
     7381 tctttacgcg gctgaacatg aggtcacccc tcggtcaagt cggaggcgga gcataccatc
     7441 ggcccgatgg cgaccataat ggccccccgg ttggccgacg tcgtcgcgcc cgacgtccgt
     7501 cgtcgccccg ggtagggagg aacctgatgc accgtccact tgcgctcgta gccgcattag
     7561 cgctggcggc gtgctcccca tcagcctccg gcccgtcagc cgatggtgcg gcgagcgcga
     7621 gcgcggtggc cagccaggtc gcggaggcgg ccggcaccat caccatcgtc gagggcatgg
     7681 ccgtcgacgg gcccgggctc accatcccgg aagccctgaa ctctggcgag gcggatccat
     7741 tgctggtcaa cggcatcctg ctccaggacg ccgagggcac gatttggctc tgccagtcgc
     7801 tgtcggactc gtcgccgcgc gcgtgcgccg gcgcctttct cgcggtcgag gacttcccgg
     7861 aaggcgtcgg cgacctcgac cccgcgaacg cggacgtgac cggactccag gaagaggatg
     7921 gcgtggtctg gctcgaggat caccagctgt acgggaccgt cagcccctca aactgattta
     7981 ggggctggag ctcgcgtccg cgctcccgct ctcggagggg ctgccgccgg ccgacggctc
     8041 cgcactcgcg tcagcgctgc cactcggcgc cgggccgacc gaaggcaccg gcgatggcgg
     8101 aggaaacagg atggtgggca cgttcagcac gtccaggacg aacatcccga tgaagatcgc
     8161 gatcacgacc acgacccagc cgcgtgccag ccagtcgttc cgaggcggca gcgcgccccg
     8221 gtagccggca tcggacggtt tgagggtgct cacaagcgcg gaagcctagc cgattcccac
     8281 gcgccgctgc gtcaggcggc gttgcgcatg gaggggatga gcagccccac gagtccggcg
     8341 gccaccgcca ccgcgccgcc cagcatcagg gtctcgcccg ccccgatgat cccggccacg
     8401 atcccggaca ccgccatggc cgtggccatg accccgaaca cgagcgcctg gcgagaggtc
     8461 acgacccggg cgaacagggc ctgcggcgtg cgctcctgga acagggtgat gttcgggatg
     8521 acgaaggcca tgttcgtgac cccgatcagg aagaacaggc caatcgcgat gaccggatcg
     8581 gtcacctggc cgaccacgat gaaggtgagg cccatgccga tgaagcccgc gatggccatc
     8641 ggtcccttcc tgaccccgcc cagcagcccg ccgatgacga ggccgcccac gatgctgccc
     8701 aggccgatgg cggtcatcag cagggcgtag ttctcgatga acggaatcgt ggactggtcc
     8761 agcacggtct gggcgtacag gagcgagcag gtgacctcgg tcccgaccgc gacctgggcc
     8821 atggtgctga ccagggtgtt cgcccgcagc tcggcctggt gggcgaggaa cgagatgccc
     8881 tcccggacgt cgcgccacag ctgacggaag cccagccggc tcgcgacccg ctcggagcgg
     8941 gccacgtgca tgagtgcgag gagcgcggcg gagatgaggt acgtccccga atcgaggacg
     9001 aacgcggcgc cgatgatgct tgccagggcc gtgaccatga gcccggccag cgggtagccc
     9061 accaggtcgg ccagggtctc ggtcacgctg gtggcgctgt tggcggccat caggtcctcg
     9121 ccctccacga tctgcgggat gagcgcgtcc ttggacgggc ggaacaggag ccccacggtc
     9181 gccaccgaga aggcgacgac gtacacgagc cggatgtcga cgttgaacgc gagcgggatg
     9241 gtcaggacca gcgcggcgcg gatgagatcc gcggcgatca tcgtccgtcg tcgatcccat
     9301 cggtcggcca gggccccggc ccacgggcca aagatcacgt tcgggatggc gatcgaggcc
     9361 atcgtgaagc ccacgtccag ggctgtgccc cgggtggcga ccaggaagcc gagcgcaacc
     9421 tggttgatcc ggtcgcccat caggctgacc agctgcccgc tccagagcag ggcgtagttg
     9481 cggttggtgg ccaggcggag atacggggac tgccgctcct cctcttcctc gccgccaacc
     9541 cctatccccg ctcccggcag gacggggacc ggctcggcag ggagagcgat ggcattcgcg
     9601 taggccactc ccagctggaa ctcgccggcc ggcatcggtc gcgccgggct ggagcccagc
     9661 gccgccgacg cgcgcgaggc caccggccgg gtcatggccg cggagatggc ccacacgatg
     9721 cccatccaca gcaggacgtc gcccacgctg accacgtcgc ggaggaccgg gatggggatg
     9781 ggcaggacgt cgccgaacat cccgccctgg cggacgaact cggcagtgga gttggcgatg
     9841 aggaggacgt gatacgggtc gcccaccagg tcggcggcgg tcagcccggc ggcgagcagc
     9901 gagccctcga agaccggcat cttc
//
LOCUS       DASYGU010000280         4924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1434547, whole genome shotgun sequence.
ACCESSION   DASYGU010000280 DASYGU010000000
VERSION     DASYGU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434547"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VGO32_08115"
     CDS             complement(<1..858)
                     /locus_tag="VGO32_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEV7200750.1"
                     /translation="MNRPGAAVLPGLLGGVLLGIAAADAGLLSAALPIVGMAGAGAWM
                     IMRVSGRHAGWVVGVFGLAFLLGTAVGGLRGAAGASPTGPRSVASLPAGQPVRVAAVV
                     VDEPMPRGDSLDVVVGDIRVAGHTAAGNLLVRVPRSVVVAAGDEIAAEVTIRARDPAD
                     ADEASYRERLRRQGIGAIATGWAAQVVGHRSSHVADAFAGVRRWLLDGLVRTVPEPEA
                     SLGAGILLGVRAGIDPAIRDAFAVAGLSHVVAISGWNVAIVVALIASATRRLRERMGP
                     SVPALVAASA"
     gene            complement(868..1455)
                     /locus_tag="VGO32_08120"
     CDS             complement(868..1455)
                     /locus_tag="VGO32_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV7200751.1"
                     /translation="MTRFDLVTVVAVIGAVVLAGAASLALLITAVPSASDPSPIADPW
                     ASVEPAEEDGGELVIDIEGGVNEPGIHRLPAGSRVADALAAAGGYADSADLGAAATSL
                     NLAAELVDGQQIVVPVIGADPDPDAGGGDGGLVNLNRATRSELEALPGIGPVTAGKII
                     SARSEQPFATLDELVSRDVLTARQLEQIADLVTVP"
     gene            complement(1577..4147)
                     /gene="leuS"
                     /locus_tag="VGO32_08125"
     CDS             complement(1577..4147)
                     /gene="leuS"
                     /locus_tag="VGO32_08125"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV7200752.1"
                     /translation="MTPRAERLDPAAYEVKWRERWERDELYRARDDVPGPFYLLTMYP
                     YTSGDVHIGHWYASTGPDVVARMHRMQGAHVMMPMGFDSFGLPAENAAIDRGVHPADW
                     TKANIDRMRAQFQTMGASWDWSREVIASDPSFYRWTQWLFLQFYRAGLAYKAMAPVDW
                     CPKDQVVLAREQVLGPERRCWRCDTPVIKRDLEQWFFRITKYADELLDFSQLDWPEPI
                     LLMQTNWIGRSEGAEIDFPVAPVTAGSDAGAVEPIRVFTTRPDTTFGATFMVLAPEHP
                     LVARLTTDERRAEVEAYVAAARRQSEIDRLAAERERDGVFIGSYAVNPLTDEPVPIWI
                     ADYVLPGYGTGAIMGVPAHDARDFDFAQRYDLPIEVVVAPRGAEPSSDAAFTAHTDDE
                     VLVNSGEFTGMSASAAIGAITAHLAERGLGAPAVSYKIRDWLVSRQRYWGAPIPIVYC
                     DVHGPQPVPEAELPVLLPRDVDFAATGVSPLQSHDGFLAATCPVDGGPARRETDTMDT
                     FVDSSWYYLRYTSPQNEDAAWDSEAMRHWMPVNLYTGGAEHAVLHLLYSRFFVKALRD
                     MGHLAFDEPFLALRNQGQILGADHQRMSKSRGNVANPDDLVAQYGADTVRLFLLFIGP
                     WDQGGPWSATGIEGVHRFLTRVWNQAQPDRGAGERRPAPSVPGMPPRSLAGEIPGSGK
                     TAADDLLAAIAGGSRSLRRITHQTIAAVTQDYGLLHFNTAISKLMELSNAIGEAMEGN
                     AGGEAVDEAVDTLILLLAPAAPHIAEELWERRGRPYSIHLHAWPVADADLAAEDLIEL
                     PVQVNGKLRDRLQVAPGTSAEEIERLALASERVQAYLDGHEAVRVVQIPGRLVNVVTR
                     "
     gene            complement(4144..4737)
                     /locus_tag="VGO32_08130"
     CDS             complement(4144..4737)
                     /locus_tag="VGO32_08130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7200753.1"
                     /translation="MTPTSTFISERIAADGRPWILRHAHPEDAEPLAALYAAVRAEGR
                     WLVTPSNAISAPSEAFFIAELIRADETAVVVAEADGTIVGNALVMTDRDVSSRHVGVL
                     SVTVADGWRDVGLGTALVAATQEWVRDRHLTRLALSVFPDNARAIAVYARAGFVREGV
                     RRRQYRQPDGTYRDELLMAWFPESGAADRWEAGEGTP"
     gene            complement(4839..4914)
                     /locus_tag="VGO32_08135"
     tRNA            complement(4839..4914)
                     /locus_tag="VGO32_08135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4879..4881),aa:Ala,seq:ggc)
BASE COUNT          768 a         1760 c         1710 g          686 t
ORIGIN      
        1 cgcgctcgcc gccaccagcg ccggcacgct gggccccatg cgttcgcgga gtcgacgggt
       61 ggcggacgcg atgagcgcga ccacgatcgc gacattccag ccgctgatcg cgaccacatg
      121 cgacagcccg gctaccgcga atgcgtcgcg aatagcgggg tcgatccccg cccggacacc
      181 aagcaggatg ccggcgccga gggacgcctc cggctcagga acggtgcgga cgagtccgtc
      241 cagaagccat cggcggacgc cggcaaatgc gtccgccacg tgcgaggacc gatggccgac
      301 aacctgcgcg gcccagcccg tggcgatggc gccgatgccc tgtcgccgga ggcgctcgcg
      361 atagctggcc tcgtcggcat cggccggatc ccgggcgcga atagtcacct ccgccgcgat
      421 ctcgtcgccc gcggccacaa cgaccgaccg tgggacccga acgaggagat tcccggccgc
      481 cgtgtgccca gccacccgga tgtcgcccac cacgacgtcg agcgagtcgc cgcgcggcat
      541 tggttcgtcg acgaccaccg cggcgacccg aacgggctgg ccggccggca gcgatgccac
      601 gctccgtggg ccggttggcg atgcacccgc cgcgccgcgc aatccgccca ccgccgtgcc
      661 gaggaggaac gccagcccga acacccccac cacccagccg gcatggcgac cggacacccg
      721 catgatcatc caggcgccgg cgcccgccat gccgacgatc ggtagtgctg cgctcagcag
      781 gcccgcatcg gcggccgcga tgccgagcag cacgccgccg agcagcccag gcaggacggc
      841 cgcgccaggc cgattcatcg gaccggccta ggggacggtc acgaggtcgg cgatctgctc
      901 gagctgacgg gcggtcagga catcacggga aacgagctcg tccagggttg cgaacggctg
      961 ctcggagcgg gcgctgatga ttttcccggc cgtcaccggt ccgatgcccg gaagcgcctc
     1021 gagctcggac cgtgtcgccc ggttgaggtt cacgaggccg ccatcacccc cgcccgcgtc
     1081 gggatccggg tccgctccga tgacagggac cacgatctgc tgcccgtcga ccagctccgc
     1141 ggcgaggttc aggctcgtcg ccgccgcccc gaggtccgcg ctgtcggcat agccgccggc
     1201 agcggccagg gcatcggcca cccgcgaacc ggccggcagg cggtgaatcc ccggctcgtt
     1261 cacgccgccc tcgatgtcga tcaccagctc gccgccgtcc tcctcggctg gctcgacaga
     1321 tgcccacggg tcggcgatcg gggatgggtc ggaggccgac ggcaccgccg tgatcagcag
     1381 ggcgaggctg gccgccccgg ccagcaccac cgcaccgatc accgccacga cggttaccag
     1441 gtcgaatcgg gtcatgccgc tccctcgacg gaggcgagcg acgcgaatgg tctgcgtccg
     1501 agacccgacg cccgggcact cgacgccggg cctcggaagc agattcggaa gctacgggct
     1561 cgtcgttacc ccctggttac cgggtgacga cgttcaccaa ccggccgggg atctgcacca
     1621 cgcggacggc ctcgtggccg tcgaggtatg cctggacgcg ctccgaggcg agcgccagtc
     1681 gctcgatctc ctcggcgctc gtgcccggcg ccacctggag ccggtcgcgg agcttgccgt
     1741 tcacctgcac cgggagctcg atgagatcct cggcggcaag atccgcgtcc gccacaggcc
     1801 atgcgtggag gtggatcgaa tagggccgac cgcgacgctc ccacagctcc tccgcgatgt
     1861 gcggcgcggc gggggccagg agcaggatca gggtgtccac cgcctcgtcg accgcctccc
     1921 cgccggcgtt cccctccatc gcctcaccga tcgcgttcga cagctccatg agcttggaga
     1981 tcgcggtgtt gaagtgaagg agcccgtagt cctgggtcac cgccgcgatc gtctggtggg
     2041 tgatccgtcg cagcgaacgg ctgccgccgg cgatcgccgc cagcaggtcg tcggccgcgg
     2101 tcttgccact tcccgggatc tcaccggcca gagagcgagg cggcatgccc gggaccgatg
     2161 gcgcgggccg ccgctccccg gcaccacgat ctggctgggc ctggttccaa acccgggtca
     2221 ggaaccgatg gacgccctcg atgccggtgg cggaccaggg accgccctgg tcccacggac
     2281 cgatgaacag caggaacagg cgaacggtgt cagccccgta ctgggccacg agatcgtcgg
     2341 gattggccac gttgccgcga ctcttgctca tccgctgatg gtcggcgccc aggatctggc
     2401 cctggttgcg caacgcgaga aatggctcgt cgaaggcgag gtggcccatg tcgcgcaggg
     2461 ccttcacgaa gaaccggctg tacagcaggt ggagcacggc gtgctccgcg ccgccggtgt
     2521 acaggttcac cggcatccaa tgccgcatcg cctctgaatc ccaggccgcg tcctcgttct
     2581 gcggcgaggt gtagcggagg tagtaccagc tcgagtccac gaacgtgtcc atcgtgtccg
     2641 tctcgcgccg ggccggcccg ccgtcgaccg ggcaggtggc ggccaggaag ccgtcgtgcg
     2701 actgaagggg ggagacgccg gtcgccgcga agtccacgtc gcggggcagc aggacgggga
     2761 gctccgcctc ggggaccggc tgcgggccgt ggacgtcgca gtacacgatc gggatcggcg
     2821 ccccccagta ccgctgccgg ctgaccagcc agtcgcggat cttgtacgag acggcgggtg
     2881 ccccaagacc gcgctcggcg agatgcgcgg tgatggcccc gatcgccgcc gacgcgctca
     2941 tccccgtgaa ttcgcctgaa ttgacgagca cctcgtcatc ggtatgggcc gtgaacgcgg
     3001 cgtcggacga cggctcggcg ccgcgcggcg cgacgacgac ctcgatcggc aggtcatagc
     3061 gctgcgcgaa gtcgaagtcg cgggcgtcgt gggctgggac gcccatgatc gcccccgtcc
     3121 cgtagccggg caggacgtag tccgcgatcc agatcggtac gggctcatcg gtaagtgggt
     3181 tgacggcgta cgagccgatg aacacgccgt cgcgctcccg ctccgcggcc agccggtcga
     3241 tctccgactg gcgccgcgcg gcagcgacgt aggcctccac ctcggcccgc cgctcatcgg
     3301 tcgtcagccg tgcgacgagg gggtgctccg gggcgaggac catgaatgtc gccccgaacg
     3361 tcgtatccgg gcgagtggtg aagacccgga tcggctcgac cgcccccgca tcgctcccag
     3421 ccgtgacggg tgccaccggg aagtcgatct cggcaccctc cgatcggccg atccagttgg
     3481 tctgcatgag caggatcggt tcgggccagt cgagctgcga gaagtcaagc agctcgtcgg
     3541 cgtacttcgt gatccggaag aaccactgct ccaggtcacg cttgatgacc ggcgtgtcgc
     3601 accgccagca gcgccgctcc gggcccagga cctgctcgcg ggcgagcacg acctggtcct
     3661 tggggcacca gtccaccggc gccatggcct tgtaggccag gccggccctg tagaactgga
     3721 ggaagagcca ctgcgtccag cggtagaacg acggatcgga ggcgatgacc tcgcgcgacc
     3781 agtcccacga cgcacccatc gtctggaact gggcgcgcat gcggtcgatg ttggccttcg
     3841 tccaatccgc cgggtggacg ccgcggtcga tggcggcgtt ctcggccggg agcccgaacg
     3901 agtcgaagcc catgggcatc atcacgtggg caccctgcat ccgatgcatg cgggccacga
     3961 catcaggtcc ggtcgaggca taccagtgac cgatgtgcac gtccccgctg gtatacggat
     4021 acatggtcag caggtagaaa ggacccggga catcgtcccg ggcgcggtac agctcgtcac
     4081 gctcccagcg ctcgcgccac ttgacctcgt aggcggcagg atccaggcgc tcggcgcgcg
     4141 gggtcatggc gtcccctcgc cggcttccca gcggtcagcc gccccgctct ccgggaacca
     4201 ggccatgagc agctcgtcgc gatacgtccc gtcaggctgg cggtattggc gtcggcggac
     4261 gccctcgcgc acgaacccgg ctcgcgcgta gaccgcgatg gctcgggcat tgtcggggaa
     4321 gacgctcagc gcgagccggg tgaggtgtcg gtcgcggacc cactcctggg tcgccgccac
     4381 cagggccgtg cccagaccca cgtcccgcca gccgtcggcg accgtgaccg acaggacgcc
     4441 gacgtggcgc gagctcacgt cgcggtcggt catgaccagc gcattgccca cgattgtgcc
     4501 gtcggcctcg gccacgacca cggctgtctc gtcggcacgg atgagctcag cgatgaagaa
     4561 cgcctcggag ggggcactga tcgcgttcga gggcgtgacc agccagcggc cctcggctcg
     4621 gacggcggca tacagggcgg cgagcggctc cgcgtcctcg ggatgggcgt ggcgcaggat
     4681 ccacggccgg ccatcggcgg cgatccgctc cgaaatgaag gtgctggtcg gggtcacggg
     4741 ctccgctccg ctctgatatc ggtctgagta caacgcgacc ccgcttcgtc aggacgaagg
     4801 gggtcagcct cgcggtacca ccatgacttg accgatggtg gtggagctgg ggggactcga
     4861 acccccgacc ccctgcatgc catgcaggtg ctctcccagc tgagctacaa ccccacggtc
     4921 ggtc
//
LOCUS       DASYGU010000281         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_406917, whole genome shotgun sequence.
ACCESSION   DASYGU010000281 DASYGU010000000
VERSION     DASYGU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406917"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..539
                     /locus_tag="VGO32_08140"
     CDS             105..539
                     /locus_tag="VGO32_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV7200754.1"
                     /translation="MRWMAGIVLALWIAMGLLLTLQPVHPAPGQVVSDNGIPFRTIAI
                     YLANGESPFWVRQFAGNLFLLLPVGLLGPMVVPALNGWLRLFTVALAMSLAIEVAQLW
                     IPNRSADVDDVMLNVAGGMLGYAIFTLFRLGSRRPSPVADGR"
     gene            complement(555..>2520)
                     /locus_tag="VGO32_08145"
     CDS             complement(555..>2520)
                     /locus_tag="VGO32_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7200755.1"
                     /translation="TRVDIPGYLKANSEGNWEEATRILKRSLPFPAILGRVCPAPCET
                     HCRREEVDTAIAIRDSHRYCADRVLEVDAPAPLPWPKESDTGRRVAIVGSGPAGMTVA
                     YYLQLRGHHCDVFEADPEQGGMLRYGIPEYRLPKGWMDRELNHVWELGATFKPNMRLG
                     RDFHLAELLDQGYDAIFVGIGCYNPNEMGIPNEDADGTVNALENLYNSTRGIPVPQLK
                     GARVVVIGGGFTAIDCTRTSLRQGAREVNLVYRRDLKDMPAQDEVHEAIEEGANVTFQ
                     AAPVRVVTDARGQVTGVEFQKMHLGEPDEKGRRRPEPMPGTEFIVECDTVLGAIGQGP
                     ELGWVETEPENIRSGLEISRQGRLVVDPNTFRTGVPKIFAAGDVNLGATTVVEAIGDG
                     RRASYAMDYWLRGHDLDDPQVRRVVSEPQPSFLTIVPFTDDVKEPQAIMGKKGADERR
                     DNFDEYEYGYTHEQAMGEAARCLQCTCEAIGHCDLREAAIEYETTLNMAIDERSIQDN
                     PYVGVNHGYGRDETHSFILRDYSRCIDCGRCAQVCKDIVGAGCYDFIGKGFDSLVTTA
                     DFVSLNETPCVSCGRCAETCPVGALMPRPRILETYQLDVSRCIFCGICADACPYDALR
                     CGPEFEFSEYQRDLPMLDILEMSDRERPTR"
BASE COUNT          346 a          852 c          874 g          448 t
ORIGIN      
        1 atcatccatt tcgtcggtcg gagggtagta gaggcatgcg ccaggtcgcg gcagatgaac
       61 tataggcagg attccaccac gcctgacaga ctgatccggt gacgatgcgc tggatggcgg
      121 gcatcgtgct cgcgctgtgg atcgcgatgg ggctgctgct gaccctgcag ccggttcacc
      181 ctgcccccgg ccaggtggtc agcgacaacg gcattccgtt ccgcacgatc gcgatctacc
      241 tggccaacgg cgaatcgccg ttctgggtcc gccagttcgc gggcaacctc ttcctgctgc
      301 tcccggtggg cctgctcgga ccgatggtcg tgcccgccct gaacggctgg cttcggctct
      361 tcacggttgc gctggccatg agcctcgcga tcgaggtcgc ccagctgtgg atcccgaacc
      421 ggtcggccga tgtggacgac gtgatgctga acgtggccgg ggggatgctc ggctacgcga
      481 tcttcaccct gttccgcctc ggttcacggc gtcccagccc ggtggccgac ggtcgctgac
      541 ggggctccgc ccgactagcg ggtggggcgc tcccggtccg acatctcgag gatgtcgagc
      601 atgggcaggt cgcgctggta ctcgctgaac tcgaactccg gtccgcagcg gagcgcgtcg
      661 taggggcagg cgtcggcgca gatgccgcag aagatgcagc ggctgacgtc gagctggtag
      721 gtctccagga tccgcggccg cggcatgagc gcgccaaccg ggcacgtctc cgcgcatcgg
      781 ccacaggaca cgcacggcgt ctcgttgagg ctcacgaagt cggcggtggt gaccagtgag
      841 tcgaagccct tgccgatgaa gtcgtagcag ccggcgccca cgatgtcctt gcacacctgg
      901 gcgcaccggc cgcagtcgat gcaccggctg tagtcacgca ggatgaacga atgggtctcg
      961 tcccggccgt agccgtggtt gacgccgacg tacgggttgt cctggatcga ccgctcgtcg
     1021 atcgccatgt tcagggtggt ctcgtactcg atcgcggcct cgcgcaggtc gcagtggccg
     1081 atcgcctcgc acgtgcactg caggcagcgg gccgcctcgc ccatcgcctg ctcgtgggtg
     1141 tagccgtact cgtactcgtc gaagttgtcc cggcgctcat cggctccctt cttgcccatg
     1201 atcgcctggg gctccttcac gtcatcggtg aaggggacga tggtcaggaa cgacggctgc
     1261 ggctcggaga ccacccggcg gacctgcggg tcgtcgaggt cgtggccacg gagccagtag
     1321 tccatcgcgt atgaggcgcg acggccgtcg ccgatggcct cgaccacggt cgtggcgccg
     1381 aggttgacgt cgccggcggc gaagatcttg gggacgccgg tcctgaacgt gttcgggtcc
     1441 accaccaggc gaccctggcg gctgatctcc agcccgctgc ggatgttctc cggctcggtc
     1501 tcgacccagc ccagctccgg cccctggccg atggcgccca gcacggtgtc gcactcgacg
     1561 atgaactcgg tgccgggcat cggttccggt cggcgtcggc ccttctcgtc cggttcgccg
     1621 aggtgcatct tctggaactc gacgccggtc acctggccgc gggcatcggt cacgacccgg
     1681 accggggcgg cttggaaggt cacgttcgca ccctcctcga tggcctcgtg cacctcgtcc
     1741 tgggccggca tgtccttgag gtcgcggcgg tagacgaggt tgacctccct ggcgccctgg
     1801 cggagggagg tccgggtgca gtcgatggcc gtgaagccgc cgccgatgac caccacccgc
     1861 gcgcccttga gctgcgggac cgggatccct cgggtgctgt tgtacaggtt ctcgagcgcg
     1921 ttgaccgtcc cgtccgcgtc ctcgttcggg atccccatct cgttcgggtt gtagcaaccg
     1981 atccccacga agatcgcgtc gtagccctgg tccagcagct cggcgagatg gaagtctcgg
     2041 ccgaggcgca tgttcggctt gaacgtcgcg cccagctccc acacgtggtt cagctcgcga
     2101 tccatccagc ccttgggcag gcggtactcc gggatcccgt agcggagcat gccgccctgc
     2161 tcgggatcgg cctcgaacac gtcgcagtgg tgtccgcgaa gctggaggta gtaggcgact
     2221 gtcatcccgg ccgggcctga gcccacgatc gcgacccgtc gcccggtgtc cgactccttg
     2281 ggccatggca gcggcgcggg cgcgtccacc tcgagcacgc ggtccgcgca gtagcggtgc
     2341 gagtcgcgga tggcgatcgc ggtgtccacc tcctcgcgcc ggcagtgcgt ctcgcacggg
     2401 gccgggcaga cgcggccgag gatggccggg aaggggagcg accgcttgag gatccgggtc
     2461 gcctcctccc agttgccctc cgagttggcc ttcaggtagc ccgggatgtc cacccgggtc
//
LOCUS       DASYGU010000282         6671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1589242, whole genome shotgun sequence.
ACCESSION   DASYGU010000282 DASYGU010000000
VERSION     DASYGU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589242"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VGO32_08150"
     CDS             <1..1076
                     /locus_tag="VGO32_08150"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV7200756.1"
                     /translation="EGPEIRSRFSELELEAREASLDDLAAVTRRLLDAGAVGAEPIPK
                     AVRALGPTATGPPDVVVPAFEPDDPAGWAVRAALADGVLRILRHDPGTRMGSVEDLHQ
                     LRVGVRRLRSDLRTLGPLLEEHWAPGLEADLRALGNRLGDVRDFDVQLARIQTEHPDL
                     AAELEPLSADLHQRHATARAALLDELRSDRYAQLLERLVVLARDPELSPAGFEPSGIA
                     LPRLADTAWRRLERRADPIVEAEEPTNAQLHRVRIATKRARYAAETAARALPEDQAEE
                     ARRFADRLGELQDLLGRHQDAVVSSAQAHAAAAEHGDDLALVVAAGRVMEREIGNARR
                     ERRRLARMWASIRKKKRRRWMRR"
     gene            1073..2014
                     /locus_tag="VGO32_08155"
     CDS             1073..2014
                     /locus_tag="VGO32_08155"
                     /EC_number="3.1.3.-"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NUDIX hydrolase/histidine
                     phosphatase family protein"
                     /protein_id="HEV7200757.1"
                     /translation="MSTPIIRAAGGVVWRPTETASGDAGVEVAVVHRPRYDDWSLPKG
                     KLAPGEPEVEGAVREVLEETGYRVRIGRPLGETRYIKDVAGVHRLKVVHWWAMRAETG
                     TFTTNDEIDDLRWLSLEGAAALVTRDSDRVVLERFAHGPMPTRTVLLVRHGSAGSAST
                     WTGDDQARPLDGQGWAQADGLARLLARFDIAEVVSADVARCTQTVAPLAEALALTVRA
                     DPVLSEVAYPGREDEAVTLVRSIGGARDGVACSQGAVIPDLLRRLVEHDDRVVPEPIA
                     NRKGSVWALTYSEGRLLEMDYIPPPDTAVEEDVPVPA"
     gene            2044..3021
                     /locus_tag="VGO32_08160"
     CDS             2044..3021
                     /locus_tag="VGO32_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200758.1"
                     /translation="MPDQPSATPDSAEPAIRTIGLTKHYSGGRHRPGRGLGPIRALND
                     VSLEVESGEIFGFLGPNGAGKSTMIRTLLGFLHATSGSARVLGMDVVRDSVALRGRVG
                     YLPGGIALYGSLAGTASLDYYADLQQREPVKRTELAERLQLSSADLKRRVRDYSRGMR
                     QKIGVIQALQHDPELAILDEPTEGLDPLMQLAFYEILEDLRAEGRTIFMSSHYLGEVE
                     RICNRVAIIRAGHLMAVQHVDELLARRKRRVSLLWRGTAPDLEGVPGLEDVVVSRDRI
                     TATLSGDVAPFIRAIASPSLVDLLIEPARLEDVFYEFYAGEDTGEGGAP"
     gene            3018..3818
                     /locus_tag="VGO32_08165"
     CDS             3018..3818
                     /locus_tag="VGO32_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7200759.1"
                     /translation="MSWTVLRQSLYWQRSRLIAVGLVALAWGALIPTFYVTFQDLFEG
                     LIEGGFPENLLNFGSGSLFTLGGTMTIGFEHPIGIGLVAIFAVGAAANAVAGEREAGT
                     LEVLLSRPLSRRRFYASVGASVKIEAAIVLALMLAGQTASIIALGVQDEIEVAWIPMV
                     WVMGLLLWAAFAAFSLAASVSFDRRGPAMALSLAYLGVNYFLEILGTFLPDLDWTQEY
                     SLFHHFNPGEIVTGNLDPVDILIFAVAIILPIIWALVVFPRRDLAAPA"
     gene            complement(3815..6463)
                     /locus_tag="VGO32_08170"
     CDS             complement(3815..6463)
                     /locus_tag="VGO32_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7200760.1"
                     /translation="MPNLPTGTVTFLFTDIEGSTRLLETLGDGYVPVLERHHALLREA
                     IATGDGTEVGTEGDAFFAVFPSAPKAVAAAGDAQRALAAEPWPPNVAVRVRMGLHSGE
                     GVLGGDSYVGLDVHRAARVAAAGHGGQVLLSGATQALAAGSLPAGVTLVDLGQHRLKD
                     LSRPEHLWQLAVHGLATEFPPPKTLDATPNNLPLQLTSFLGRQREVSDVLALLAANRL
                     VTLTGPGGTGKTRLALQAAADASDQFAGGVYFVALEPISKADLLLPTIAQASGMPDPG
                     SASVQRLAEHFGDRAFLLVLDNFEQVNAAAPVIGDLLAQAPKLTILATSRSPLRVYGE
                     HEYPVPPLGLPDPQHMLELEQFTQFESVALFVERAVAVKPGFSVDNANAPAVAEICVR
                     LDGLPLAIELAAARVRVLTPQAILDRLSDRLGLLSGGARDLPERQQTLRGAIAWSHDL
                     LESDDRRAFARISVFAGGATLEQVEEVCFEPPDRGMALDVVSSLVDKSLLREESTGDE
                     PRFRMLETIRQFAAEQLAAGGEAELLLGRHAAAILAFAERGAGEVMGTDGRAWLDRFE
                     LERDNLRAAIRWALEGPHTETALRLLTACWRYWQIRGYLTEARDYAQRTLALPDAHDF
                     PEVREAALEAAGGIAYWQGDIDAARVWYQENLDLARARHDDRAEANALYNMTFTYAWQ
                     VDAQDEARAIAEESLEVNRRLNDQQGIGRALWAVASTYYFKTDLGPARRLVDEALAIF
                     REIDDRFMIGWTLYIRGLIQLNHDAAGMRADLTEAYGYFRATDDITGYVLVFDAFAAL
                     AHATGDDLTAARLAGFATATEHLAGSGLGAANRELAGFDPETLRIDSAEFAAAFADGQ
                     HLELADVERLALGPDYVAPSPAPVAG"
BASE COUNT          952 a         2359 c         2356 g         1004 t
ORIGIN      
        1 tggaggggcc ggagattcgg tcccgcttca gcgagctcga gctcgaggcc cgcgaagcct
       61 cgctcgacga cctggcggcc gttacccggc gactgctgga cgccggcgcg gtgggcgccg
      121 aaccgattcc gaaggcggtc cgggccctcg ggccgaccgc gaccggcccg ccggacgttg
      181 tggttcccgc gttcgagccg gatgaccccg cgggatgggc cgtccgcgcg gcgctcgccg
      241 atggggtgct ccgcatcctg cgccacgacc cgggcaccag aatgggctcg gtcgaggacc
      301 tgcatcagct ccgcgtcggc gtccggcggc tgcggagcga cctccggacg ctcggaccac
      361 tgctcgagga gcattgggcg ccaggtctcg aagccgacct ccgggcgctc gggaaccggc
      421 tcggcgacgt tcgcgacttc gacgtccagc tggcacggat ccagactgag catcccgacc
      481 tggcggcgga gctcgagccg ttgagcgcgg atcttcacca gcggcacgcc acggcccgcg
      541 cggcgctcct cgacgagctc cggtccgacc gttacgccca gctcctcgag cggctggtgg
      601 tcctggcccg cgaccccgag ctcagccccg cgggcttcga gccctcgggc attgcgctcc
      661 cccggctcgc ggacaccgcc tggcgccggc tcgagcgccg ggccgaccca atcgtcgagg
      721 ccgaggaacc aaccaatgcc cagctccatc gggttcggat cgccaccaag cgcgcgcgtt
      781 atgccgccga gaccgcggcc cgcgcgctgc cggaggatca ggccgaggag gcccgtcgct
      841 tcgctgatcg gctgggcgag ctccaggacc tgctcggacg gcaccaggac gcggtcgtgt
      901 cgtctgccca ggcccacgcc gccgccgcag aacacggcga cgatctcgcc ctggtggtcg
      961 cggccggccg cgtgatggag cgcgagatcg ggaacgcgcg gcgggagcgc cgacgcctgg
     1021 cccggatgtg ggcctcgatc cggaagaaga agcgccggcg ctggatgcgg cgatgagcac
     1081 gcccatcatc cgggcggccg gtggggtcgt gtggcgaccg accgagaccg cgtcgggaga
     1141 cgccggggtc gaggtggcgg tcgtccaccg gccgcgctac gacgactgga gcctgcccaa
     1201 gggcaagctg gcccccggcg agcccgaggt cgagggcgcg gtgcgcgagg tgctcgagga
     1261 gaccggctat cgcgttcgga tcggccgtcc gttgggcgag actcgctaca tcaaggacgt
     1321 cgccggtgtg catcggctca aggtcgtcca ctggtgggcg atgcgagcgg agacggggac
     1381 gttcaccacc aacgacgaga tcgacgacct ccgctggctg tcgctggagg gcgcggccgc
     1441 cctggtcacc cgcgacagcg accgcgtcgt gctcgaacgg ttcgcgcacg gaccgatgcc
     1501 cacccggacc gtgctcctcg tgcgccatgg cagcgccggg tcggcctcga cctggaccgg
     1561 cgacgaccag gcgcggccgc tcgacgggca gggctgggcg caggccgacg ggctggcacg
     1621 cctcctcgcg cgcttcgaca tcgccgaggt cgtctcggct gacgtcgccc gctgcacaca
     1681 gacggtggct ccgctcgccg aggcgctcgc gctcacggtg cgcgccgacc cggtcctgtc
     1741 ggaggtcgcc tatcccggtc gagaggacga ggccgtgacg ctcgtgcgca gcatcggcgg
     1801 cgctcgggac ggggtcgcct gcagccaggg tgccgtcatt ccggacctgc ttcgtcgcct
     1861 ggtcgagcac gacgaccggg tggttcccga accgattgcc aaccgcaagg gcagcgtctg
     1921 ggccctgacc tacagtgagg gacggctgct ggagatggac tacatccctc cgccggacac
     1981 cgcggtcgag gaggacgtgc cggtcccggc ctgatccacg gcgatgcgct aggctccggg
     2041 ccgatgcctg accagccgag cgccacgccc gattccgccg agccggcgat caggaccatc
     2101 ggactgacca agcactacag cggcggacgg catcggcccg gccgcggcct tggcccgatt
     2161 cgcgcgctga atgacgtcag cctggaggtc gagtcgggcg agatctttgg cttcctcggc
     2221 cccaacggcg ccggcaagtc gaccatgatc cggaccctgc tcgggttcct ccacgcgacg
     2281 agcgggtcag ccagggtcct cggcatggac gtcgtccgag actcggtcgc cctccgtggc
     2341 cgggtcgggt acctgcccgg cgggattgcg ctgtacggct ccctcgccgg tactgcgtca
     2401 ctcgactact acgccgacct ccagcagcgc gaaccggtga agcggacgga gctcgccgag
     2461 cggctccagc tgtcatcggc cgatctcaag cgacgggttc gggactacag ccggggcatg
     2521 cgccagaaga tcggcgtcat ccaggccctc cagcacgatc ccgagctcgc gatcctcgat
     2581 gagccgaccg agggcctcga tccgctcatg cagctcgcgt tctacgagat cctcgaggac
     2641 ctgcgggccg agggtcggac catcttcatg agctcgcact acctgggcga ggtcgagcgc
     2701 atctgcaacc gggtggccat catccgggcc gggcacctga tggccgtcca gcacgtggac
     2761 gagctccttg cccgccgcaa gcgccgggtc agcctgctct ggcgcgggac ggcgcccgac
     2821 cttgagggcg tcccgggcct cgaggacgtc gtcgtctcgc gggaccggat cacggccact
     2881 ctctccggcg acgtggcacc gttcatccgg gccatcgcct cgccttcgct ggtggacctg
     2941 ctcatcgagc cggcccgcct ggaggacgtc ttctacgagt tctacgccgg cgaggacacc
     3001 ggcgagggcg gcgctccatg agctggaccg tgctgcgcca gtcgctctac tggcagcgat
     3061 cgcggctcat cgccgtcggc ctggtggcgc tcgcctgggg cgccctgatc ccgaccttct
     3121 acgtgacctt ccaggatctg ttcgaagggc tgatcgaggg cgggttcccg gagaacctgc
     3181 tgaacttcgg atcgggcagc ctgttcaccc tgggcggaac gatgaccatc ggcttcgagc
     3241 atccgatcgg catcggcctg gtcgcgatct tcgccgtcgg agcggctgcc aatgcggtcg
     3301 cgggcgagcg cgaggcggga acgctcgagg tgctcctgtc ccggccgctc tcccgccgac
     3361 gcttctacgc gtccgttggc gcttcggtga agatcgaggc cgcgatcgtg ctggccttga
     3421 tgctcgctgg ccagacggcg agcatcatcg ccctcggcgt gcaggacgag atcgaggtcg
     3481 cctggatccc catggtctgg gtgatgggcc tgctgctgtg ggcggcgttt gccgcgttct
     3541 cgctggccgc cagcgtctcg ttcgaccgcc gcgggccggc catggccctg agcctggcat
     3601 acctgggcgt gaactatttc ctcgagatcc tcgggacctt cctgccggac ctggactgga
     3661 cgcaggagta ctccctgttc caccacttca accccggtga gatcgtgacc ggcaatctcg
     3721 acccggtgga catcctcatc ttcgcggtgg cgatcattct gcccatcatc tgggcgctcg
     3781 tcgtcttccc ccgccgcgac ctggcggctc cggcctaacc cgcgaccggc gcgggggacg
     3841 gggcgacgta atcgggaccg agggcgagcc gctccacgtc cgccagctcc agatgctgac
     3901 cgtcggcgaa cgccgcggcg aactcggcac tgtcgatccg cagcgtctcc ggatcgaacc
     3961 cggccagctc ccggttcgcg gcacccaggc cgctgcccgc caggtgctcc gtcgcggtgg
     4021 caaagcccgc cagacgcgcc gccgtcaggt cgtcgccggt cgcatgggcg agggcggcga
     4081 atgcgtcgaa gacgaggacg tagccggtga tgtcgtcggt cgcgcggaag tacccgtagg
     4141 cctcggtgag gtcggcccgc atgccggccg catcgtggtt cagctggatc aagccccgga
     4201 tgtacagcgt ccagccgatc atgaatcgat catcgatctc gcgaaatatg gcgagcgcct
     4261 cgtcgaccag ccgccgtgcc ggtcccaggt cggtcttgaa gtagtacgtg ctggccaccg
     4321 cccatagtgc gcggccaatc ccctgctggt cgttcagccg ccggttgacc tccagggatt
     4381 cctcggcgat cgctcgcgcc tcgtcctgcg cgtcgacttg ccacgcgtag gtgaaggtca
     4441 tgttgtacag ggcattggcc tccgcgcggt cgtcatgccg ggcgcgcgcc aggtccaggt
     4501 tctcctggta ccagacacgg gccgcgtcga tgtccccctg ccagtacgca atcccgccgg
     4561 cggcctccag cgcggcctcc cggacctctg gaaagtcatg cgcatcgggc agagcgagcg
     4621 tccgctgggc atagtcccga gcctcggtca ggtagcccct gatctgccag tagcgccaac
     4681 acgcggtcaa cagccggagc gccgtctcgg tgtgtggtcc ctcgagcgcc cagcggatgg
     4741 cggcgcggag gttgtcgcgc tcgagctcga atcggtccaa ccacgctcgg ccgtcggtgc
     4801 ccatgacctc gcccgctccg cgttcggcga aggccaggat ggcggcggca tggcgaccga
     4861 ggagcagctc agcttcgccg ccggcagcca gctgttcggc cgcgaattgg cgaatggtct
     4921 cgagcatccg gaaccggggc tcatcgccgg tcgactcctc gcgcagcagg ctcttgtcca
     4981 ccagggagct caccacgtcg agggccatcc cccggtcggg aggttcgaag cagacctctt
     5041 cgacctgctc aagggtggcg cctccggcga agaccgagat ccgcgcgaac gccctccggt
     5101 cgtcgctctc gagcaggtcg tggctccacg cgattgcccc gcggagggtc tgctggcgct
     5161 ccggtaggtc gcgagcgccc ccggacagca gcccgagccg gtcgctcagc cgatcgagga
     5221 tggcctgcgg ggtcagaaca cggacgcgag ccgcggccag ctcgatggcc agcggcagtc
     5281 cgtcgagccg gacgcagatc tcggccacgg ccggggcgtt cgcgttgtcc accgagaacc
     5341 cgggcttcac ggcaactgct cgctccacga acagcgccac cgactcgaac tgggtgaact
     5401 gttcgagctc gagcatgtgc tgcggatcgg gcagaccgag cggtgggacg gggtattcgt
     5461 gctcgccgta gacccggagg gggctccggc tggtggcgag aatggtcagc ttcggcgcct
     5521 gggccaggag gtcgccgatg accggtgctg cggcattgac ctgctcgaaa ttgtccagga
     5581 cgaggaggaa ggcccgatcc ccgaagtgct cggccaggcg ctggaccgac gccgacccgg
     5641 gatcgggcat cccgctcgcc tgcgcgatgg tggggagcag gaggtcggcc ttggagatgg
     5701 gctcgagcgc cacgaagtac acgccgccgg cgaactggtc ggaggcatcg gccgcggcct
     5761 gcagcgcgag ccgcgtcttt cccgtcccgc cgggcccggt cagggtcaca agccggttgg
     5821 cggcgagcag ggccagaacg tccgatacct cgcgctggcg gcccaggaac gacgtgagct
     5881 ggagcgggag gttgttcggg gtcgcgtcca gggtcttggg cggcgggaat tcggtggcca
     5941 ggccgtggac cgccagctgc cagaggtgct ccggcctgga gaggtccttg agccgatgct
     6001 gcccgaggtc gacgagggtg actcccgcgg gaaggctgcc cgcggccagg gcctgggtcg
     6061 caccggagag gagcacctgg ccgccgtggc cggcggcggc gacccgggct gcgcgatgga
     6121 cgtcgagtcc cacgtagctg tcaccgccca gcacgccctc cccgctgtgg agccccatcc
     6181 gaacccgaac cgcgacgttc ggcggccacg gctcggcggc gagcgcccgt tgggcatcgc
     6241 cggcggcggc aaccgccttc ggagccgagg ggaacaccgc gaagaacgca tctccctcgg
     6301 ttcccacctc ggtgccatca ccagtcgcaa tcgcttcgcg caggagcgcg tgatggcgct
     6361 cgaggaccgg cacataccca tcacccagcg tctcgagcag ccgcgtgctg ccctcgatat
     6421 cggtgaacag gaaggtgacg gttccggtcg gaaggttcgg catcgggtgg gggtagtttc
     6481 gggcttcggt tctgccccgg caaggcagaa cggataccgt accgaccgat gagcaacgag
     6541 ctggcgccgc ccctcgaccg gctcggctca cgtcccgtca cggccgcggt cctcggcgcg
     6601 ctggccatct ccttcaccgg ggtcaacgtc cgcctggcca acgtggagcc ggccaccgcc
     6661 gccgtgttcc g
//
LOCUS       DASYGU010000283         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_356647, whole genome shotgun sequence.
ACCESSION   DASYGU010000283 DASYGU010000000
VERSION     DASYGU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356647"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..841
                     /gene="radC"
                     /locus_tag="VGO32_08175"
     CDS             197..841
                     /gene="radC"
                     /locus_tag="VGO32_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV7200761.1"
                     /translation="MPRDRAPAERLAAVGAVSFTNDELLALVLAGPSALATARILLDA
                     AGIDRLPGMLVAELVGYAGIGTRRAAAVVAAFELARRATSHWPEPGWRVRAPGDVAER
                     LLPEMRHLEREELLVCLLNTRNVVLGRSTVYVGNLAGSSVRIGEVYREAVRRQAAAVI
                     VAHNHPSGDPSPSADDLRITADLADAGRLLDIELLDHLVIGHDRWVSLRAAGAM"
     gene            complement(872..2137)
                     /locus_tag="VGO32_08180"
     CDS             complement(872..2137)
                     /locus_tag="VGO32_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7200762.1"
                     /translation="MTHRAVRRPSRESLVLLAGGALLVVGCTSASASPSLQTSEAATP
                     SIAPTAEPSVEVSPPGLTSGPDALAVEPIASGFVNATAVTNAGDGSGRLFVVQREGQI
                     RIVNPGGTVETAPFLDITGLVLAGNERGLLGLTFHPDYEDNGRFFVSYTARPDGPEII
                     AEYRVSSDPSVADPASGRILISVPDPAANHNGGALAFGPDGYLYISMGDGGGQNDQYQ
                     NGQNLNSLLGKLLRIDVDGTPTTGRTYAIPSSNPFVDGGGAPEIWAYGLRNPWRISFD
                     REWGDLFIGDVGGGRWEEINRQPADGPGGLNYGWPVMEGRHCLADTCTISGYVQPIAE
                     YSHDTGGCSVIGGYVYRGSAQPDLAGVYIFGDWCTGTVFTLQVDEGTITPKPVLDSGL
                     AMSAFGEDDSGEIYLVDFAGGGLYRVVLP"
     gene            2185..>3483
                     /locus_tag="VGO32_08185"
     CDS             2185..>3483
                     /locus_tag="VGO32_08185"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEV7200763.1"
                     /translation="MTTTRASLRRLASVHGATGAEIGTAQTIVIERVEPELDGGRYPA
                     KRVQGDWFAVTADIIADGHDQLAAALLIRAEDETGWQEIPMRPGRYDRWTGRVRLRRN
                     VRHIYTLEAWRDEVASWSERLSRKAGLRLGIEFELAEGGQLLAVVARRARQARERDDA
                     AVLDSARERLAAAPDPRSAARIARERSLLAAGARHPDRSTATRYDHELTLVVDRPEAA
                     TGVWYELFPRSQGRQPGHATTLVEAEWRLPHLKRLGFDVVYLPPIHPIGTTNRKGRNN
                     AEQARPGDVGSPWAIGSADGGHEAVAPELGGLEAFQHFRRATERLGMEVALDLAIQAS
                     PDHPWVHDHPEWFRHAPDGTIRYAENPPKRYQDVYPFDFRGPDRDARVALWEAWRELV
                     LTWAERGVRMFRVDNPHTKPVVFWERLFARLRTAAPDAIFL"
BASE COUNT          516 a         1234 c         1207 g          526 t
ORIGIN      
        1 ctcaccgctt gcgctgccgc ccatcttcct gctgggcctg atcctggccg tcgcctacat
       61 cacgacccgc agcctgcccc tggtcatcgg ggtacacgcc acgttcaact gcctctcgct
      121 ggccgtgctc ttcctcgcga ccgcgtgaaa atgcggcggt aagtcgccag caagtctcgt
      181 cgagcgatcc tcgggaatgc cccgtgaccg cgcccccgcc gagcgcctgg ctgccgttgg
      241 ggccgtgtca ttcacgaacg acgaactctt ggccctggtt cttgcggggc catccgcgct
      301 ggcaacggcg cggatcttgc tggatgccgc cggcatcgac cgtctgccgg ggatgcttgt
      361 tgcggagctg gtggggtacg ccgggatcgg gacgcgacgc gcggcggccg tggtagccgc
      421 gttcgagctg gcccgccgag ccacatccca ttggccggag cccggctggc gggtccgggc
      481 cccgggcgac gtcgccgagc gactcctgcc cgagatgcgc caccttgagc gcgaggagct
      541 gctggtgtgc ctgctgaata cccgcaacgt cgtgctcggc cgctcgacgg tctacgtcgg
      601 caatctcgcc gggtcatcgg tccggatcgg tgaggtctac cgtgaggccg ttcgacgcca
      661 ggcagcggcg gtcatcgtcg cccacaacca cccctccggc gacccgtcgc cgtccgccga
      721 cgatctccgg atcacggccg accttgccga tgccggccgg ctcctcgaca tcgagctcct
      781 cgaccacctg gtcatcggtc acgaccgatg ggtgagcctc cgagcggcag gcgcgatgtg
      841 atcgactgga ccagatgacc gaccggtcag gtcagggaag gaccacccga tacaggcccc
      901 cgcccgcgaa gtcgacgagg tagatctcac ccgagtcgtc ctccccgaac gcgctcatcg
      961 ccagcccgct gtcgagcact ggcttgggcg tgatcgtgcc ctcatccacc tggagcgtga
     1021 agacggtgcc agtgcaccag tcgccgaaga tgtagacccc ggcgagatct ggctgagccg
     1081 acccgcggta gacgtagccg ccgatgaccg agcagccgcc ggtgtcatgg gagtactcgg
     1141 caatcggctg gacatagccg ctgattgtgc aggtgtcggc gaggcagtga cgaccctcca
     1201 tcaccggcca gccatagttg aggccacccg ggccgtcggc cggctggcgg ttgatctcct
     1261 cccaccggcc accgccgaca tcgccgatga acaggtcgcc ccactcccgg tcgaagctga
     1321 tccgccacgg gttgcgtagg ccataggccc agatctccgg agccccgccg ccgtcgacga
     1381 acgggttgct cgaggggatc gcgtaggtcc gtccggtggt cggcgtgcca tcgacatcga
     1441 tccgcaggag cttgccgagc aggctattca ggttctgccc gttctggtac tggtcgttct
     1501 ggccgccgcc gtctcccatc gagatgtaga ggtagccgtc gggcccgaat gccagggcgc
     1561 caccgttgtg gttggcggcc gggtcgggga ccgagatcag gatccgaccc gacgccgggt
     1621 cggcgacgct tggatcggac gacacgcgat actcggcgat gatctccggc ccgtccggtc
     1681 gggccgtata cgagacgaag aagcggccgt tgtcctcgta gtcgggatgg aaggtcagtc
     1741 cgagcagccc ccgctcattc ccggccagaa ccaggccggt gatgtcgagg aacggggcgg
     1801 tctccaccgt gccgccaggg ttcacgatcc ggatctggcc ctcgcgctgg acgacgaaca
     1861 gcctgcccga cccatcgccc gcgttcgtga ccgccgtcgc attcacgaaa ccgctggcga
     1921 tcggctccac ggccagggcg tccggtccgg acgtgagccc cggtggggac acctcgacgg
     1981 acggctcggc ggtgggcgcg atcgacgggg tcgcggcctc tgacgtttgc agcgatgggc
     2041 tcgcggaggc gctcgtgcag cccacgacga ggagagcccc gccggcgagc aggacgagcg
     2101 attcccgaga tggccgccgc acggcccggt gcgtcatggg ttcatacagc gtacccgctc
     2161 ggatggggta gggttcgaag gccgatgacc accactcgag cgagcctgcg ccgcctggca
     2221 tcggtccacg gcgcgacggg cgccgagatc gggaccgcgc agaccatcgt catcgagcgg
     2281 gtcgagcccg agctggacgg cggccgctac ccggccaagc gagtgcaggg cgactggttc
     2341 gcggtcaccg ccgacatcat cgccgacggt catgaccagc tcgccgcggc gttgctcatc
     2401 cgggccgaag acgaaaccgg gtggcaggag attcccatgc gtccgggccg ctatgaccga
     2461 tggactggac gcgtccgcct gcgccgcaac gtgcgccaca tctacaccct cgaggcgtgg
     2521 cgcgacgagg ttgccagctg gtccgagcgg ctgagccgca aggccggcct gcggctggga
     2581 atcgagttcg agctggcgga aggcggccag ctgctcgcgg tcgtcgcgcg gcgggcgcga
     2641 caggcccgag agcgggacga cgccgccgtc ctggactccg cacgtgagcg gctggccgcg
     2701 gcgcccgacc cacgttccgc ggcgcggatc gcccgggagc ggtcgctgct ggcggccggc
     2761 gcgcggcacc cggaccggtc gaccgcgacc cgctacgacc atgagctgac gctcgttgtt
     2821 gaccggcccg aggcggccac cggcgtgtgg tacgagctgt tcccgcgctc gcagggccgg
     2881 cagccgggcc acgcgaccac cctggtcgag gccgagtggc gcctgcccca cctcaagcgg
     2941 ctcgggtttg atgtcgtgta cctgcctccc atccatccga tcggaaccac gaaccgcaag
     3001 ggccggaaca acgccgagca ggcccgaccg ggggatgtgg gcagcccatg ggccatcggc
     3061 tccgccgacg gcggccacga ggccgtcgcc cccgagctcg gcgggctcga ggcgttccag
     3121 cacttccggc gagcgaccga gcgtctgggc atggaggtcg cgctcgacct tgccatccag
     3181 gcctcgcccg accacccatg ggtgcacgac caccctgaat ggtttcggca tgcgcccgac
     3241 ggcacgatcc ggtacgcgga gaacccgccc aagcgctacc aggacgttta tccgttcgac
     3301 tttcgtggtc ccgaccgtga tgcccgggtg gcgctgtggg aggcctggcg cgagctggtc
     3361 ctgacctggg ccgagcgggg ggtgcgcatg ttccgggtcg acaacccgca caccaagccg
     3421 gtcgtcttct gggagcggct cttcgcccgc ctccggacgg cagcgccgga cgcgatcttc
     3481 ctg
//
LOCUS       DASYGU010000284         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1179740, whole genome shotgun sequence.
ACCESSION   DASYGU010000284 DASYGU010000000
VERSION     DASYGU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179740"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1506)
                     /locus_tag="VGO32_08190"
     CDS             complement(<1..>1506)
                     /locus_tag="VGO32_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7200764.1"
                     /translation="EVLFTSGTTGEPKGAMLTHRNLSSNARELPKVFPIGPNERMLSV
                     LPLSHIFEQSPGFLVPLHAGASVVYPVSRQPSVLLRTFKDFKVSILLIVPQGLKLLDN
                     AIERKVDQGGKRATFEKLHRWAANAPRFVQRLLFRPVLSSFGGRLHTVGVGGSALDPE
                     LAARWEEMGINVLQGYGATELGPLVSFTRKENNRLGTVGQAVGGVEMRIAEDGEVMVR
                     SPGVFAGYWEDPERTAEVLEPDGWYHTGDIGEISADGFLTLRGRKKDMLAMPDGTKVY
                     PEDIEAVLDKDERVRDATVVGFPVGPDLKIHAVLLLDDADQAEAIVKDANQVVGAHQQ
                     IRAHSVWPDEDFPRTHTLKVKKREVIARLEADAAAAAQSSASVAPVEAAVVPAVVGTL
                     GTVIGLVAAIIELSPEAIQPTARLSSDLNMDSLHRIELLGVIEEELGVFVDDSALDPE
                     ATVGQIAALVDASREAKHETGILAWPLHPLTRVVGLTFQELVTSPILNAFYR"
BASE COUNT          225 a          515 c          533 g          233 t
ORIGIN      
        1 cggtagaacg cgttgaggat gggggaggtg accagctcct ggaaggtcag gccgaccacc
       61 cgggtcagcg gatggagcgg ccaggccagg atgccggtct catgctttgc ctcgcgcgag
      121 gcatccacca gcgccgcgat ctgcccgacc gtggcctccg ggtcgagtgc cgagtcgtcc
      181 acgaacacgc ccagctcctc ctcgatcacc ccgagcagct cgatgcggtg gagcgaatcc
      241 atgttcaggt cggacgacag gcgtgccgtg ggctggatgg cctccgggct gagctcgatg
      301 atggcggcca ccaggccgat gaccgtgccc agggtgccga cgaccgccgg gacgacagcc
      361 gcctccaccg gggcgaccga cgcggacgac tgggcggccg cggccgcatc ggcctccagc
      421 cgggcgatga cctcccgctt cttgaccttc agggtgtggg tccgtgggaa gtcctcgtcg
      481 ggccagacgg aatgcgcgcg gatctgctgg tgggcgccga cgacctggtt ggcgtccttc
      541 acgatggcct cggcctggtc ggcatcgtcg agaaggagga cggcgtggat cttgaggtcc
      601 gggccgaccg ggaagccgac cacggtcgcg tcgcgcacgc gctcgtcctt gtccaggacc
      661 gcctcgatat cctcgggata gaccttggtg ccgtccggca tggcgagcat gtccttcttc
      721 cggccgcgga gggtgagaaa cccatcggcg ctgatctcgc cgatgtcgcc ggtgtggtac
      781 cagccgtcgg gctcgaggac ctcggccgtc cgctcggggt cctcccagta gccggcgaac
      841 acaccggggc tgcggaccat gacctcgccg tcctcggcga tccgcatctc gaccccgccc
      901 accgcctggc ccaccgtgcc cagccggttg ttctccttgc gcgtgaagct gaccagcggc
      961 cccagctcgg tggcgccgta gccctggagg acgttgatcc ccatctcctc ccagcgcgcg
     1021 gccagctccg gatcgagcgc cgaccccccg accccgaccg tgtgcagccg cccgccgaag
     1081 ctggacagca ccggccggaa gaggagccgc tggacgaagc gcggcgcgtt cgcggcccag
     1141 cggtggagct tctcgaaggt cgcccgtttg ccgccctggt ccaccttgcg ctcgatcgcg
     1201 ttgtccagca gcttcaggcc ctgcgggacg atgagcagga tgctgacctt gaagtccttg
     1261 aacgtccgga gcaggaccga cggctggcga ctgaccggat agacgacgct ggcgcccgcg
     1321 tggaggggca cgaggaaccc cggcgactgc tcgaagatgt gggacagggg caggaccgag
     1381 agcattcgct cgttgggacc gatggggaag accttgggca gctcgcgcgc gttggaggac
     1441 aggttgcggt gggtcagcat cgcgcccttt ggctcaccgg tcgtgcccga ggtgaacagc
     1501 acttcg
//
LOCUS       DASYGU010000285         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_973825, whole genome shotgun sequence.
ACCESSION   DASYGU010000285 DASYGU010000000
VERSION     DASYGU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973825"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VGO32_08195"
     CDS             <1..464
                     /locus_tag="VGO32_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200765.1"
                     /translation="LSVLGFLVMSGSVFHRRRTPLAAVLAVLAIILTASMVSADPKGN
                     NGTVKVHSSGGDETAMNMANDPHVTCPFHFHFYFGDAGQTQTWWVADDAGNGVAGGTY
                     TADAAGEAQSGDVVVPGAGHYTVSWNGRNEQNVKHKTFWVDGDCGGGGGEG"
     gene            complement(547..1332)
                     /locus_tag="VGO32_08200"
     CDS             complement(547..1332)
                     /locus_tag="VGO32_08200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7200766.1"
                     /translation="MPESDDRLTEQRYWDDYWAGVELPLARVHRPGQLYINEILDVFD
                     RHLPRDPSLTAIEIGGTPGAYLAYVHRTFGYGVHCLDYSERGCAATRENFGLLGIPGV
                     VHRADVFTDDLELPSFDVVYSLGLIEHFTDLTGVVERHVRLVRPGGWLLLGVPDFLGI
                     NGWFLRRLGPRVLAQHQLETMDLENWDSFERRFQLTTLFKGYVGGFEPSIFLRREDET
                     RANLVPYTIARALTRLLHTHFRPLRRLNASWLSGYAMAVYRVP"
     gene            complement(1325..>2069)
                     /locus_tag="VGO32_08205"
     CDS             complement(1325..>2069)
                     /locus_tag="VGO32_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7200767.1"
                     /translation="TSLAAVAAITERIQLGPLVAATSFHNPAMLAKKAATLDEISGGR
                     LILGLGAGWNETEYRAYGYPFDHRVSRFEEAFTIIRTLLHDGRCDFAGDYYRVTDAEL
                     IPRGPQPNGPPLMVGSMGERMLSITLPHVQSWNAWFTWFGNSPEGYPPLRDQIDAACR
                     AVGRDPATLERTLAVLVTLPGGEGRPQGSHDRDPVAPISGEPEVLADALRAFAAEGVG
                     HIQLVIDPITTDSIRALAPALALFDRGHA"
BASE COUNT          359 a          660 c          728 g          322 t
ORIGIN      
        1 tcttatccgt attggggttc ttggtcatgt caggttctgt attccatcgt cggcgcactc
       61 cgctcgctgc ggtcctcgca gtgcttgcca tcatcctcac cgcttcaatg gtgtccgccg
      121 atccgaaggg caacaacgga acggtgaagg tgcacagcag cggcggcgac gagaccgcca
      181 tgaacatggc gaacgatccg cacgtcacgt gcccgttcca tttccacttc tacttcggcg
      241 acgccgggca gacccaaacc tggtgggtcg ccgatgacgc tggtaacggc gtggcgggcg
      301 gaacctacac ggccgatgcg gccggtgagg ctcagtccgg cgacgtcgtg gtcccggggg
      361 ccggccacta cacggtgtcg tggaatggcc gcaacgagca gaacgtcaag cacaagacct
      421 tctgggtcga tggggactgc gggggcggcg gcggcgaggg ctgaccgtga cgggccggcc
      481 cggcgaaacg ggccggcccg acccatccag gcgtcgagca gggattcctg acccgccgcc
      541 ttagcgtcac ggcacgcggt acaccgccat cgcgtagccg ctgagccacg atgcgttgag
      601 tcggcgcaag ggccggaagt gggtatgcag gaggcgggtg agcgccctgg cgatcgtgta
      661 cggcacgaga ttcgcccgcg tctcatcctc acggcggagg aagatgctcg gctcgaagcc
      721 gcctacgtag cccttgaaca gggtggtgag ctggaaccga cgctcgaagg agtcccagtt
      781 ctccagatcc atcgtttcca gctgatgctg ggcgagaacc cgggggccaa gtcgcctcag
      841 gaaccaccca ttgatgccca ggaaatccgg gacgccgagg aggagccacc cgcccggcct
      901 gacgaggcgg acgtgacgct cgaccacgcc cgtcagatcg gtgaagtgct cgatcagccc
      961 gagcgagtac acgacgtcga acgacgggag ctcaaggtcg tcggtgaata cgtccgcgcg
     1021 gtgaacaaca ccaggaatgc ccagcaaccc gaagttctcg cgggtcgcgg cgcatccgcg
     1081 ctcggagtaa tcgaggcagt ggacgccgta gccaaaggtg cggtgcacgt aggcgaggta
     1141 cgcccccggg gtacccccga tttcgatcgc ggtcaggctg gggtcgcgag gcaggtgacg
     1201 atcgaagaca tcgaggatct cgttgatgta cagctgcccg ggacgatgga cccgcgcgag
     1261 cggcagctcc acgcccgccc agtagtcgtc ccagtagcgc tgttcggtga gccgatcgtc
     1321 cgactcaggc atggccgcgg tcgaagaggg cgagggccgg agcgagagcg cgaatgctgt
     1381 cggttgtgat cgggtcgatg accagttgga tgtgaccgac tccttcggcc gcaaaggcgc
     1441 gtagagcgtc cgccagcacc tcgggctcgc cggagatggg cgccaccgga tcgcgatcgt
     1501 ggcttccctg cggccgtccc tcgccaccgg gcagggtgac cagcaccgcg agtgtccgct
     1561 ccagcgtggc tgggtcgcgg ccgaccgcgc ggcacgcggc gtcgatctgg tcgcggagcg
     1621 gcggatagcc ctccggcgag ttgccgaacc aggtgaacca cgcgttccag gactggacgt
     1681 ggggcaaggt gatcgacagc attcgctccc ccatcgatcc caccatcagc ggcggtccgt
     1741 tgggctgggg accgcgcgga atgagctcgg catcggtaac ccggtagtaa tcgccggcaa
     1801 agtcgcagcg gccatcgtgc agcagggtcc ggatgatggt gaacgcttcc tcgaaccggc
     1861 tgacccggtg atcgaagggg tagccgtatg cgcggtactc ggtctcgttc cagcccgcgc
     1921 ccaggcccag gatcagccgg ccgccgctga tctcgtccag ggtggcggcc ttcttggcca
     1981 gcatcgccgg gttgtggaac gaggtggcgg ccaccagcgg gccgagctgg atccgctccg
     2041 tgatcgcggc caccgcggcg aggctggtc
//
LOCUS       DASYGU010000286        20537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_99118, whole genome shotgun sequence.
ACCESSION   DASYGU010000286 DASYGU010000000
VERSION     DASYGU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 20537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20537
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99118"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VGO32_08210"
     CDS             <1..766
                     /locus_tag="VGO32_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEV7200768.1"
                     /translation="EFTEAVDRVIAGPERRSRIITEDEKKIIAYHEGGHAVVQRVLPK
                     CDPVTKVTIISRGMALGYTMSLPQEDRYLHSKTEFEDKIAGMLGGHVSEELVFGDTTT
                     GASNDIEKATNLARAMVTQYGMSEALGPLAFGKKEEMIFLGREISEQRNYSDEVAAKI
                     DAEVRAIIDQAYARARTALTEHRAVLDRLADLLVEKETIEAAEFESLWEGVLPPRNIG
                     GPTPVGAAAGDDAPEPAGEDAKPTPRRRRGPAPQPA"
     gene            927..2006
                     /locus_tag="VGO32_08215"
     CDS             927..2006
                     /locus_tag="VGO32_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7200769.1"
                     /translation="MIDESPAPQAAEADWKLEANGVALDPRSALEVLTNLNERVAEGR
                     VGDLQPVATGFVPFDKAIGGGLRSGELLLIGGAQGSGKTTLALQMARNMAASGQATVL
                     YVCFEHEEDYLVQRMIAMESALATLPKKTGAVKIQDVRQEVLSTFEAAAEAGQVAKLG
                     ANPRLRPSLERIARYGNSMYLMRGTATDSTIENLRALVEARRGDDGRRLVLFVDYLQK
                     VPVLPEPPDETEKVTRVVNGLKDIALACEIGVVAIVAADREGLKAKRLRNHHLRGSSA
                     INYEADIILILNDKYNIVAKVNIEFNPYQAQRYRDWVVATVEKNRGGQDNVDLEWQKL
                     FQYSCFDPNGRVTAEKLIEERLYTE"
     gene            2022..2456
                     /locus_tag="VGO32_08220"
     CDS             2022..2456
                     /locus_tag="VGO32_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV7200770.1"
                     /translation="MDAYRVLQVTPECEDEVIHGAFRALAMKYHPDRDGTQFAARRMA
                     ELNQAYALVRNPQLRARHDAARRRAAIPEATRTGGPAPVAPPRAAAAGTRIAFGRYAG
                     WTLRDLARQDPDYLRWLSRHSSGIRYRTEIYQILTKQEPSVA"
     gene            2484..3200
                     /locus_tag="VGO32_08225"
     CDS             2484..3200
                     /locus_tag="VGO32_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV7200771.1"
                     /translation="MTNPVRAVFLDVGETLMRPNPSWEHVYAGVFTDWGVPVEMAELQ
                     VALHAVYQSGGYGFDGSVEPSPEQSHRRLVEMDKWAFDQIGLDPMPDGFFEDLAARFQ
                     DPATWQVFADVRPAMEGLRSRGLILGVVSNWAWQLPELLDGLGLGPMLDFVAASARIG
                     YDKPHPGIFQWALDRAGVPSETVVHVGDHLDADVAGARGVGIDAVLVDRRGRYDPPPD
                     GVPVVTSLEGLLPLIDARLA"
     gene            3184..3774
                     /gene="thpR"
                     /locus_tag="VGO32_08230"
     CDS             3184..3774
                     /gene="thpR"
                     /locus_tag="VGO32_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV7200772.1"
                     /translation="MLASPEAWRCFVAVPVPAAIRSVLEPAVAAWRAESGAPDLRWTD
                     PDGWHVTLAFLGPTEPNVVPGLEDALGRAVLPFPPPRFATGRLGAFPRAQAAQSIWLG
                     LDDPSGVLGQLATAVQAAVLEPDRRRPLRAHLTIGRSRARRGEPLGGWLESRMVPTVD
                     VVVHDVVLYRSHLGRGPARYEEVARIPVGGAGSARG"
     gene            3767..4300
                     /locus_tag="VGO32_08235"
     CDS             3767..4300
                     /locus_tag="VGO32_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEV7200773.1"
                     /translation="MDEKPPRPPRASVVAVIGDGDPRGPDADRVLDWAEEVGQLLGRA
                     GATVVTGGLGGVMRAASKGAAAAGGLTIGILPGSDADEANEYVKLPIATGLGVVRNLV
                     VVTAADAVLAVGGRHGTLSEIGLALRMGRNVVALGSWRVESDQRIGGPRLHRARDPRE
                     AAQLALELAAAGPIQPA"
     gene            4317..4940
                     /locus_tag="VGO32_08240"
     CDS             4317..4940
                     /locus_tag="VGO32_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7200774.1"
                     /translation="MVERVLVLAREHVPGGCDFLGLRAGSTDELAALRDAIRKHGRWV
                     DRPIAEDDPSAKQVIPYVVVRDRSTHPERVFLMERTAAGGDPRLYSRASIGVGGHLNP
                     PDSASDDARDPLTAGLHREWQEELVADWDPEFMLVGFLNDDRNPVGAVHLGVVFVVEA
                     AGRSIEVRERDKLSGRLATLDELGAAWPRLETWSQLVGEALWGQPMG"
     gene            4976..6328
                     /locus_tag="VGO32_08245"
     CDS             4976..6328
                     /locus_tag="VGO32_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEV7200775.1"
                     /translation="MAGLRRGVLGALVLLGLAGSAAAGQEPVAEIELHGVIDQVNAAF
                     IEEAVAAASDAGAVAVLLKIDSPGGELTSTDRILQAILNSDIPVITWVTPSGGQAASA
                     ATFVTLAGDVAAMAPLTTIGSASVVGSGGEELPETLARKITNDLVAKIRGLADAHGRN
                     ADWAESAVRDASNIGAEEAASLEPPVVDLVAETTDELFAAIDTGERSDGYAYHFNEEP
                     LPELSGRPLMTISMNVGQELLHILSDPNIAFLLFTIGFYGILAELFHPNFLSGIVGAI
                     AIVLAFIGSNSLPLNVGGLILIAIGLGLFALELFVPSYGLLTVGGIIAFLLGAFALYT
                     GVDGTDAIQVELNPLLVMLPLVLAIGVIVLVARGLVDVRRRSAPTAPMHALLGATGIA
                     STPIAPTGIVMAHGEAWTARSRRGSIRPGESLTVVGVDGLELIVEPAGGRVDPPNEKE
                     "
     gene            6332..7240
                     /locus_tag="VGO32_08250"
     CDS             6332..7240
                     /locus_tag="VGO32_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011752498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV7200776.1"
                     /translation="MEFLIPLAIVIIALAIVLWLIFSVVHIVREYERLVVFFLGRLQG
                     AKGPGLVLLIPVIQQAVKVDLRESFLEIPQQSCITKDNAPISIDFLVYSKVFEPMITV
                     TAVTDFTGASQALAATTLRAVIGDIPLDDVLAKREEINHILRAKLDEVTERWGVKITS
                     VEIREIQPPRDIQDAMNRQMSAERNRRAVITESEGTRQASINVAEGDKQSTVLRAEGD
                     KQSAILRAEGERQAQALRAEGFGTALTTIFGAASGVDEKTMQIQYLEVLRSLAASESK
                     TWIVPMEVTELTRRISATLAGGGDER"
     gene            7351..8196
                     /locus_tag="VGO32_08255"
     CDS             7351..8196
                     /locus_tag="VGO32_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7200777.1"
                     /translation="MTPSLHAVDELEGLPGTPGELGFPHEAVRFTTDDGVTLDGWLIA
                     AERDTRAAVILLHGFSWHRVPWLSMFVPWLRERYHVLQFDFRGHGASDPAATTLGTAE
                     RRDVAAAVRLLEGRGLGPIALYGISMGGATAILAGAELPVAAVVADAAYADIRNPIAN
                     RMREGRLPLPGLGSRLVVAGAALRARTRLISPIDRVADLSPRGLLLIAPREDRLVDWQ
                     QSVRLHEAAGEPKELHVVNGAAHGEARMVAGAEYEHWVLDFLERHLDVEPATRGAQKG
                     AGGRL"
     gene            8252..9433
                     /locus_tag="VGO32_08260"
     CDS             8252..9433
                     /locus_tag="VGO32_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7200778.1"
                     /translation="MAVKVLVVDDDLNIQRVLSFTLKQEGFDVILAGDGETGVALAAE
                     QVPDLILMDAVLPGIDGYQAIAQIRGAEPAGRRAPILLLTTEADVVQRVKGLRAGADD
                     DIVKPFHPAELMARIRALLARSGLGGPRAGSVQATLRRVIVFYGAKGGVGNTTLAINT
                     AIALASEHKRRVALIDANLQFGDLRVFLDLKLDTASIVNAISEPELDTDLLRRLMVSH
                     SSGIDLLLAPAVPEQADVVMDSHRASPEALTHIIELMRGMYDYTVIDAARSLDHVTIH
                     LFDEADVVYVVLTADLSCLKNVQLVLKTMTELGYPRDKIQLVLNRSNAFTGINIANAE
                     SVLGRPIGHKLINEYRGAIGALNSGAPFMISRPGSPLAKAVSDFAVSVDKELSVVESE
                     A"
     gene            9542..11065
                     /locus_tag="VGO32_08265"
     CDS             9542..11065
                     /locus_tag="VGO32_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEV7200779.1"
                     /translation="MSASPRWTVLAETMSASIRGVEAVPVRVEVDVAFGLPGLTIVGL
                     AGSAIQEARERVRSAVRNSGFEVPARRITVNLAPADLPKEGTAYDLAIGVAMLAASGQ
                     LEADLAHTALLGELALDGSLRPVAGTLALVAAARDAGHRAAVVAQGDVVEAAAVPGIA
                     VHGARSLGDAVAHLAGVRPLAAATRTPLPPWSLPEATVDLRDVIGQGVGRRALEIALA
                     GGHNLALVGPPGVGKTLLLSSVPGLLPPLDDDEAIEVSRIYSIAGLVDRQVPLTRVRP
                     VRAPHHTTSTQALVGGGERIRPGEASLAHRGALVLDELLQFRTDSLDALRQPLESGQV
                     TIARVEGALRLPARFMLLAAFNPCPCGWLNARSRACACDPASVQRYDRRLSGPLRDRI
                     DLWVMMEEPRRVVTDAEAEDSAVVASRIGVAWDRQHDRQGAPNGTLGPDALGAERGFG
                     PDAIRSLTRLGRVLRLSPRRAHRAAWVARTIADLQGSPGVSPVHVEEALHYRPPVPA"
     gene            11062..12294
                     /gene="dprA"
                     /locus_tag="VGO32_08270"
     CDS             11062..12294
                     /gene="dprA"
                     /locus_tag="VGO32_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV7200780.1"
                     /translation="MSVAEAVGRLGAWTGLPLDQLDPERERPYWIATSQLPGIGPVAI
                     ARMLARAGSLRAAWQGGLVEQAAAELPADVRDLAAQHVRFLRQRGVPRAAAELVARGR
                     PHGIRPLTALDEDYPPSLRHLDPRPAVLWAAGDPTAWQTTTVAVVGTRRPSGYGRAAA
                     EEIGDELARAGVTVISGLAVGVDAVAHTAAVAAGGRSVAVLPSPIDRIYPPANRDLAH
                     RLRDAGALLTEVPPGRRIGRPDFARRNRIIAGMAEAVVVVEAPDRSGALLTAAAAIEL
                     GRELFAVPGPMDAVTSRGTNRLIADHAATLVTSPAALLHLIGLRRSGFTVSVRTLSEA
                     EGLVLGRLLQRSGSIEELADRTALPTSMLAAALTMLEARGLVAAYAGATFHPTLAARR
                     SGWPKGSARTASALAGSA"
     gene            12407..14023
                     /locus_tag="VGO32_08275"
     CDS             12407..14023
                     /locus_tag="VGO32_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7200781.1"
                     /translation="MGEVRRLAAHVEPFRRHRAITHLTIAVTALAVAVAVAIPAAPVI
                     QAGLQTTPRTGSILPANVGIGVAGTDAVILHFPGPMDQAAVATTIGLSPATKFSLLWA
                     GDSTSVAVVPSPRWAVDERYVIHLPAGTAMADGGALAADWRAAFTTQTAPSVTHLTVG
                     GVTGTPAGDTPIILQEVMSSTGAAGTAPEDAATDDRDSPDVTADAPIGITFSGAMDAE
                     TTEAAFHIAPDVSGSLRWEGTTMWFQPDQRLAAGTRYTISLVGARDVDGNPLGGDVSL
                     SFTTRVRAEAIKVAPALGARDVPATTSVRIVFTQPMATARTAAAFTVTDTATGRAVVG
                     RPAWSADGRTMTFVPATSLGAGHTFAAALGSGAHDADGNPVTIAWRFTTAGQVVQSYS
                     SSSVPVAAGSASMVQYALNQINAARASYGLGPLVVDSTITAVSYGHAADMLAYNYFSH
                     SSRNGATYKQRLSAGGVGYGYSGENICYLGYGGGVQATLNWCHAQFWAEPFPGGGNHK
                     DNILGSHFRRVGIGIAVGGNKVYVVWDYTD"
     gene            14089..16341
                     /gene="topA"
                     /locus_tag="VGO32_08280"
     CDS             14089..16341
                     /gene="topA"
                     /locus_tag="VGO32_08280"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV7200782.1"
                     /translation="MSGPRNTGGDLVIVESPAKAKTIERYLGDGFHVLASYGHVRDLP
                     KRGLGVDLEHDFAPRYEPLPDRKDELKRLTDAAKTANMVWLATDLDREGESIAWHIAE
                     HAGLAENRIRRVTFSEITKPAIEAAFASPRDLNLDLVDAQQARRIIDRLVGYKLSPLV
                     ASKIRRGLTAGRVQSVAVRLVVDREREIQAFTAVEYWTIAADLRRGESGAVFRVGLTK
                     IDGQKAEVGSEEEATAHVAALREASYRVTDVTRGERRQNAPPPFTTSTLQQEASRKLG
                     FSARRTMAAAQALYEGISLPGGQVGLITYMRTDSVSIAASALTEAREVIAARYGVASV
                     PERPNTFRTRSRGAQEAHEAVRPSSFARTPESVEGALKPDHARLYDLIWRRAMASQMA
                     PAVFDQVGVDVEAGRYLLHAGARKRVFDGYQAVYIEGRDEEEEAGAPKLPDLAVGEEL
                     DLVELVSDQHFTEPPPRFSEATLIRALEEHGIGRPSTYAPTMEVIRARGYVEMTDRRL
                     HPTEEAFLLTDMLTGHFADVVDPEFTARMETQLDEVAGGRREWVPMVREFWEPFSEQV
                     ARGKEEIPKNVEETDIMCPVSGHPMLKRLGRNGWFLGCSGYPECKHTMPLPGEEDPAL
                     DLPGVGETCPECGEGTLTGKRSRFGPFLGCSRYPDCKYIHRPEPDPAAAGADGAAGPA
                     LEGVGETCPECNQGILAAKRSRFGPFVGCSRYPDCKYIRKSRSGSPRAGSGRRKGAKR
                     GSRRRASPRA"
     gene            16334..17338
                     /locus_tag="VGO32_08285"
     CDS             16334..17338
                     /locus_tag="VGO32_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEV7200783.1"
                     /translation="MPDRASTPDTLESAVERFLSHLATRNAAAGTLVEYRRHVTEFGR
                     FLAGRGVDWRTPDRAIIRAWLATLAERELSASAVGSRLSATRSFYRHAVRQGWIDTDP
                     LTGVRSPRRPRRLPRVLSVDEAARLVEAPAARSETASATGPAPARRTPPDLEDALRRR
                     DAAILELLYATGMRISELAGLSLTDVDLGRRRLRVLGKGRKERDLIFGRPAATTLGAY
                     LGGARPFLAARGHGSGPDAVFLNANGGRLTARGARLVVSRWVRVAELGGRTSPHTLRH
                     SFATHLLEGGADLRTVQELLGHSSAQTTQIYTHLSDAALRSAYRSAHPRSARPSRTRT
                     "
     gene            17340..18917
                     /gene="murJ"
                     /locus_tag="VGO32_08290"
     CDS             17340..18917
                     /gene="murJ"
                     /locus_tag="VGO32_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV7200784.1"
                     /translation="MTTPRPPDLSSAAGVVVRASLVMTAAAVVSRLLGWIRLLVIGAE
                     FGATSELDAYLAAFRIPDAIFQLVVAGALSAALIPVYAGYRARGEEDEGWALASSVIN
                     LVLVALVVASGVMALAAPWLVPIIAPGFDADTTALTVQLTQIMLLSPVFIGLGAVISG
                     LLQAHGTFGPTALAPLVYNLAIIGAAVFLAPSLGIEALAAGVVVGAIGHLLVQLPALR
                     RLGPRWRPRIDLRHRGVRRVTVLMGPRFLGLAAGQINLIVSTALASSLAAGSITAFTY
                     AFQLSQLPVGIIGVSIAVALFPTLSRDAALGRASEIRRQVNASMRVLFFVSAPLAVLM
                     VILREPLASVFFEYGLFDAESAERIASALAWFAIGVPAHVVVHVLARAFYAMQDTWTP
                     VVWAVIAVISNIVLMLVLVDPMGVEGLALALSVSATLEVIGLLIALRRRLGTLDGRFL
                     LASVTRSSVAAVLAGIAMFIALGFMNVALGGASFGRLLTLLVPATVGGLVFLGTALVL
                     RAPELATLRRLVRRTPR"
     gene            18914..19969
                     /locus_tag="VGO32_08295"
     CDS             18914..19969
                     /locus_tag="VGO32_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV7200785.1"
                     /translation="MTFRPAVDADRDGWQALVGRHPSGDFLHDWGWAEVAAHDGQPQR
                     RFLLEEVGEPVAVVAAQVRPIGMSRSFWYVPHGPVTDFTAPTAADRVETLVAGLAAEA
                     RDDRAVALRLEPRIPADAAEATLLDAVAHRVSERLQVGQTRIVDLVEDDALLSGFDSD
                     TRYGVRRAERESVAITLVTDPADMDAIERLHELVRETQQRAGFRLPPIERYRVAWQAL
                     AGAGRACLVEARREGELLATGMLVVEGGQSFYLFAGSRREASGEPKRYASYAVQWAMM
                     REARRRGATRHDLWGVAPANAGPDHPWHGVGLFKKGFGGHEVTWAGSWEIVIDPLVYR
                     LRAAASRLRRRTPRDVP"
     gene            19966..>20537
                     /locus_tag="VGO32_08300"
     CDS             19966..>20537
                     /locus_tag="VGO32_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV7200786.1"
                     /translation="MSERTMGLGALADAVNPDRVVGIPVGEVTALTAQSAEATPGTAF
                     FAIPGQVHDGWAFIPEAVANGAVAVIAERETPGLVVPQILVADARHAVADAADAWFGR
                     PSERLRVLGITGTDGKSTTAFLLLDVLLAAGRRPGMVGTIDTRIGDTQTASTSRTTTP
                     EALELQALLAAMVEEGNDSVVIEATSHGLA"
BASE COUNT         2943 a         7360 c         7146 g         3088 t
ORIGIN      
        1 cgagttcacc gaggccgtgg accgcgtcat cgcgggtccc gagcgacgga gccggatcat
       61 caccgaggac gagaagaaga tcatcgccta ccacgagggc ggccacgccg tggtccagcg
      121 cgtgctcccc aaatgcgacc cggtgaccaa ggtgaccatc atctcgcgcg ggatggccct
      181 gggctacacg atgagcctgc cacaggagga ccgctacctg cactccaaga ccgagttcga
      241 ggacaagatc gcgggcatgc tcggcgggca cgtctccgag gagctcgtgt tcggcgacac
      301 gacgaccggc gcgagcaacg acatcgagaa agccaccaac ctggcccgcg cgatggtcac
      361 ccagtacggc atgagcgagg cgctcgggcc actcgcattc ggcaagaaag aggagatgat
      421 cttcctcggc cgcgagatca gcgagcagcg caactactcc gacgaggtcg cggccaagat
      481 cgacgcggag gtccgcgcga tcattgacca ggcatacgct cgggcccgca ctgccctgac
      541 cgagcaccgt gccgtcctcg accgcctggc cgacctcctg gtcgagaaag agacgatcga
      601 ggccgccgag ttcgagtcgc tgtgggaagg cgttctgccg ccgcgcaaca tcgggggccc
      661 cacgccggtc ggcgcggctg ccggtgacga cgcgccggag ccagctggcg aggacgccaa
      721 gcccacccca cgtcgccggc gcggtccggc gccgcagccc gcctgatcct cccgttcggc
      781 gcgtctgcgt cggcacccgt cgtcgattgg gacggcgcta gtcctatcgg cccattccgc
      841 atccggaaac ggtggtcgta tactcgcggc cggtgcgtcc gggcacctgt ccggaacatc
      901 gaatcacagc gaggccgacg ctccccatga tcgacgaatc gcccgccccg caggccgctg
      961 aagcggactg gaagctcgag gccaacggcg ttgcgctgga tccgcgcagc gcgctcgagg
     1021 tgctcaccaa cctcaatgag cgcgtggccg agggcagggt tggggacctc cagccggttg
     1081 ccactggctt cgttccgttc gacaaggcca tcggtggcgg gctgcgctct ggcgagctgc
     1141 tgctgattgg cggcgcccag ggttcgggca agacgaccct cgcgctccag atggctcgca
     1201 acatggccgc ctccggccag gccacggtcc tgtacgtctg cttcgagcac gaggaggact
     1261 acctcgtcca gcggatgatc gcgatggagt ccgcactggc caccctgccc aagaagaccg
     1321 gcgcggtgaa gatccaggac gttcgccagg aggtgctctc caccttcgag gccgccgccg
     1381 aggcgggcca ggtcgcgaag ctcggcgcaa acccacggct ccggcccagc ctcgagcgga
     1441 tcgcgcgcta cggcaactcg atgtacctca tgcgcggcac cgccaccgac agcacgatcg
     1501 agaacctccg ggccctggtc gaggcacggc gcggcgacga cgggcggcgc ctggtcctgt
     1561 tcgtcgacta cctccagaag gtgccggtcc tgcccgaacc gccagacgag accgagaagg
     1621 tcacccgggt cgtgaacggg ctcaaggaca tcgccctcgc ctgcgagatc ggcgtggtgg
     1681 cgatcgtggc cgccgaccgc gagggcctga aggccaagcg gcttcggaac catcacctgc
     1741 gtggcagctc ggcgatcaac tacgaggccg atatcatcct gatcctgaac gacaagtaca
     1801 acatcgtggc caaggtcaac atcgagttca acccgtacca ggcccagcgc tatcgggact
     1861 gggtagtggc caccgtggag aagaaccgcg gcggacagga caacgtcgac ctggagtggc
     1921 agaagctctt tcagtacagc tgcttcgatc cgaacggtcg ggtcacggct gagaaactga
     1981 ttgaagagcg cctctacacc gaataggacg gcgttcgcgc catggatgcc taccgggtcc
     2041 tgcaggtcac gcccgaatgc gaggacgagg tcattcatgg cgcattccgt gcgctggcca
     2101 tgaagtacca cccggaccgc gatggcaccc agttcgcggc gcggcggatg gccgagctga
     2161 atcaggccta cgcactcgtt cgcaaccctc aactgcgagc gcgacatgac gccgcccgtc
     2221 gtcgcgccgc cattcccgag gccacccgca ccggcggacc cgctccagtt gcgccacctc
     2281 gcgccgcagc ggccggcacc cggatcgcct tcggccggta tgccggctgg acgctccggg
     2341 atcttgcgcg ccaggacccc gattacctcc gctggctctc gcgccattcc tcgggaattc
     2401 gctaccggac cgagatctac cagatcctca ccaagcagga gcccagcgtc gcctaggctg
     2461 tgtcacgtgg gcctgcgtca acggtgacca atcccgttcg tgccgtgttc ctcgacgtcg
     2521 gcgagacgct catgcgcccg aatccgagct gggagcacgt ctacgcgggc gtcttcaccg
     2581 actggggcgt gccggtcgag atggccgagc tccaggttgc gctccatgcc gtgtaccaaa
     2641 gtgggggata cggctttgac ggcagcgtgg agccgagtcc cgagcaaagt catcggcggc
     2701 tggtcgagat ggacaaatgg gcgttcgacc agatcggtct ggacccgatg ccagacggct
     2761 tcttcgagga tctggccgcc cgcttccagg atcccgccac gtggcaggtg ttcgccgacg
     2821 tccggcccgc gatggagggc ctaagaagtc ggggcctgat cctgggggtg gtatccaact
     2881 gggcctggca gctgccggag ctgctcgacg ggcttggcct ggggccgatg ctcgacttcg
     2941 tcgccgccag cgcgcggatc gggtacgaca agccccaccc ggggatcttc cagtgggccc
     3001 tggaccgcgc cggcgtgcca tccgaaacgg tcgtccacgt cggggaccac ctggatgcgg
     3061 acgtggccgg cgcccgaggg gtcggcatcg acgcggtgtt ggtcgatcgc cggggccgct
     3121 acgaccctcc gccggacggc gtgcccgtgg tgacgtcgct cgaaggcctc ctgccgctca
     3181 tcgatgctcg cctcgcctga ggcgtggcgc tgcttcgtgg ccgtcccggt cccggcggcc
     3241 atccgctccg tgctcgagcc ggcggtcgcc gcctggcgcg ccgaatccgg ggcgccagac
     3301 ctccggtgga ccgatcccga cggctggcac gtgaccctcg cctttctggg tccgaccgaa
     3361 ccgaacgtcg ttcccgggct ggaagacgcc ctggggcgcg cggtcctgcc cttcccgcca
     3421 ccccggttcg cgaccgggcg cctgggtgcc tttccccgcg cccaggccgc tcagagcatc
     3481 tggctcgggc tcgacgatcc atccggtgtc ctggggcagc tcgcgaccgc ggtccaggct
     3541 gccgtgctgg agcctgatcg ccggcgaccg ctccgtgccc acctgaccat cggacgcagc
     3601 cgagctcggc gcggcgagcc gctgggtgga tggctcgagt cccggatggt gcccacggtc
     3661 gatgtcgtcg tccacgacgt ggtcctgtac cgcagccatc tcggccgtgg gccggcccgc
     3721 tacgaggagg ttgcgcgcat ccccgtgggc ggtgcaggat cggcccgtgg atgagaagcc
     3781 gccccgaccg ccccgagcat cggtggtggc ggtcattggg gacggcgatc cccgtggtcc
     3841 ggacgccgac cgagtcctcg actgggccga ggaagtgggt cagctcctgg gtcgggctgg
     3901 cgcgacggtg gtgaccggag gactgggagg cgtgatgcgc gcggcatcca agggcgccgc
     3961 cgcggccggc ggtctgacca ttggcatcct gcccggctcg gacgccgacg aagcgaacga
     4021 gtatgtgaag ctgcccattg ccaccggcct gggcgtggtc cgcaacctgg tcgtcgtcac
     4081 ggccgccgat gccgtgctcg ccgtgggcgg ccgacacggc accctgtcgg agatcggtct
     4141 cgccctgcga atgggccgca acgtggtcgc cctcgggtcg tggcgggtcg agtccgacca
     4201 acgcatcggc gggccccgac tgcaccgcgc ccgggatccg cgtgaggccg cccagctggc
     4261 actcgagctc gcggccgccg ggccgatcca gcccgcctga cgaggtcggc tcaccggtgg
     4321 tcgagcgggt cctcgtcctg gctcgcgagc acgtcccggg gggatgcgac ttcctgggcc
     4381 tgcgagccgg gtccaccgac gagctggccg cattgcgcga cgcgattcgg aaacacggtc
     4441 ggtgggttga ccgcccgatc gccgaggacg atccctccgc caagcaggtc atcccgtatg
     4501 tcgtcgttcg cgaccggtcg acccaccccg agcgcgtgtt cctcatggag cgcaccgccg
     4561 ccggcggcga cccgcggctc tacagccggg cgtcgatcgg ggtcggcggt cacctcaatc
     4621 cgccggatag cgcgtcggac gatgcgcggg atccgctcac cgcgggtctc caccgcgagt
     4681 ggcaggagga gctggtcgcc gactgggatc ccgaattcat gctggtcggg ttcctgaacg
     4741 acgaccgcaa cccggttggc gcggttcacc tgggcgtcgt cttcgtggtg gaggccgcgg
     4801 ggcgctcgat cgaggtccgt gagcgagaca agctgtcagg ccggctggcg acgctcgacg
     4861 aactgggcgc ggcgtggccc cggctcgaga cctggtccca gctggtggga gaggcgctgt
     4921 gggggcaacc catggggtga tcggcgcctg cctggggcac gcctatactc gaaacgtggc
     4981 aggcctccgc cgcggcgtac tcggtgccct tgtcctgctt ggtctggcgg gatcggccgc
     5041 ggcgggccag gagccggtcg ccgagatcga gctccatggg gtcatcgacc aggtcaacgc
     5101 cgccttcatc gaagaggcgg tcgcggccgc gtcggacgcc ggtgcggtgg cggtcctgct
     5161 caagatcgac tcgcccggcg gcgagctgac cagcaccgat cggatcctgc aggccatcct
     5221 caacagcgac atcccggtca tcacctgggt cacgccatcg ggcgggcagg cggcgtcggc
     5281 ggccaccttc gtgacgctcg ccggtgacgt ggcagccatg gcaccgctga ccacgatcgg
     5341 ctcagcgtcg gtcgtgggct cgggtggcga ggagctgccc gagacgctgg cccgcaagat
     5401 cacgaacgac ctggtggcca agatccgcgg gctggccgac gcccatggtc ggaacgccga
     5461 ctgggccgaa tcggccgtgc gggacgcgtc caacatcgga gccgaggagg cggcatcgct
     5521 cgagcccccc gtcgtcgacc tcgtggccga gaccacggat gagctgttcg cggcgatcga
     5581 caccggggag cggtcagacg ggtacgccta ccacttcaac gaggagccgc tccccgagct
     5641 gagcggtcgc ccgctgatga cgatctcgat gaacgtgggt caggagctgc tccatatcct
     5701 gagtgacccg aacatcgcgt tcctgctgtt cacgatcggc ttctacggga tcctggccga
     5761 gctgttccat ccgaacttcc tgtcggggat cgtgggtgcg attgccatcg tcctggcgtt
     5821 catcggctcg aacagccttc cgctcaacgt gggcggcctg atcctgatcg cgatcggcct
     5881 ggggctcttc gcgctcgagc tgttcgtgcc ctcatacggg ctgctcacgg tgggcgggat
     5941 catcgccttc ctgctcggcg cgttcgccct gtacaccggc gtggacggga ccgatgcgat
     6001 ccaggtcgag ctgaacccgc tgctcgtgat gctcccgctc gtgctcgcga tcggggtcat
     6061 cgtcctcgtc gcccgcggcc tggtggacgt tcgccgccgg tcggcaccga ccgcaccgat
     6121 gcatgcgctt ttgggcgcga ccgggattgc ctcgacgccg atcgcgccaa ccggcattgt
     6181 tatggcgcat ggtgaagcct ggacggcgcg cagcaggcgg gggtccattc ggccgggcga
     6241 aagcctgacc gtggtgggcg tggatggact ggaattgatc gtcgagccag ccggcggccg
     6301 agtcgacccg cccaacgaga aggagtaatc gatggagttc ctgattccgc tcgcgatcgt
     6361 gatcatcgcc ctcgcgatcg tgctgtggct gatcttcagc gttgtccaca tcgtccgtga
     6421 gtacgagcgc ctggtcgtct tcttcctcgg acggttgcag ggggccaagg gaccgggcct
     6481 ggtgctcctc atccccgtca ttcagcaggc cgtcaaggtg gacctccggg agagcttcct
     6541 cgagatcccc cagcagagct gcatcacgaa ggacaacgcc ccgatcagca tcgacttcct
     6601 cgtctactcc aaggtgttcg agccgatgat cacggtcacc gccgtgaccg acttcaccgg
     6661 cgcttcccag gcgctggcgg ccaccaccct gcgggccgtg atcggtgata tcccgctcga
     6721 cgacgtgctg gccaagcgcg aggagatcaa tcacatcctc cgggccaagc tcgacgaggt
     6781 gaccgagcgc tggggggtga agatcacgag cgtggagatc cgggagattc agccgccgcg
     6841 tgacatccag gatgcgatga accgccagat gagcgctgag cgcaaccgtc gtgcggtcat
     6901 cacggagtcc gagggcaccc ggcaggcatc gatcaacgtg gccgagggag acaagcagag
     6961 caccgtgctc cgagccgagg gcgacaagca aagcgccatc ctccgggcgg agggcgagcg
     7021 gcaggcccag gctctgcgag ccgaagggtt cggaaccgcg ctgaccacga tcttcggcgc
     7081 cgcgagcggc gtcgacgaga agacgatgca gatccagtac ctcgaggtcc tgcgcagcct
     7141 cgcggcgtcc gagagcaaga cctggatcgt gccgatggag gtgaccgagc tgactcgccg
     7201 gatctcggcc acattggccg gcgggggaga cgagcgctga gcggcgatcg atcgcgaggt
     7261 cggggcctgc tgacggcggc cggcgccctg gtcggggcgc tgaccgccgg ctatctgggc
     7321 tacgtcggcg tcgtcggctc gcgccgggtg gtgaccccgt cgctgcacgc ggtcgacgag
     7381 ctcgagggcc tgcccggcac gccgggcgag ctgggcttcc cacacgaggc ggttcggttc
     7441 acgaccgatg acggcgtgac gctcgacggg tggctcatcg cggccgagcg cgacacgcgg
     7501 gccgcggtga tcctgctcca cggtttctcg tggcaccgtg tgccatggct gtcgatgttc
     7561 gtgccgtggc tccgcgagcg gtaccacgtg ctccagttcg acttccgcgg ccatggcgcc
     7621 tccgatccgg ccgccaccac cctgggaacg gcggagcgac gggacgtcgc ggcggccgtc
     7681 cgcctgctcg agggccgggg cctgggaccg atcgcgctgt acggcatcag catgggcggg
     7741 gcaaccgcga tcctggccgg tgccgagctg ccggtcgccg cggtcgttgc cgacgccgcc
     7801 tatgccgaca tccggaaccc gatcgccaac cgcatgcgcg aggggcgcct gccgttgcct
     7861 ggcctcgggt cgcggctagt cgtggccggc gccgcgctgc gggctcgaac ccgcctcatc
     7921 tcgcccatcg accgggtcgc ggacctttcc cctcgtggcc tgctgctgat cgcaccacgc
     7981 gaggatcgcc tggtcgactg gcagcagtcg gtccgactgc atgaggccgc gggggaaccc
     8041 aaggagctgc acgtggtgaa cggggccgcg cacggcgagg cgcggatggt cgccggggcg
     8101 gagtacgagc actgggtcct cgacttcctg gagcgtcacc ttgacgtcga gccggccacc
     8161 cgcggagcgc agaagggggc gggcggccga ctataattcc gccacccgcg ccgggcgaac
     8221 caccggaccg gccgggctct cgcgagggcc catggcagtc aaggtcctgg tcgtcgacga
     8281 cgacctcaac atccagcgcg tcctgagctt caccctcaag caggaggggt tcgacgtcat
     8341 cctcgccggc gacggcgaga ccggtgtcgc cctggccgcc gagcaggttc cagatctcat
     8401 cctcatggac gcggtcctgc ccggcatcga cggttaccag gccatcgccc agatccgggg
     8461 cgcggagccc gcgggccggc gggcaccgat cctgctgctc accaccgagg ccgacgtcgt
     8521 ccagcgggtc aagggcctgc gcgcgggcgc cgatgacgac atcgtcaagc ctttccaccc
     8581 cgccgagctc atggcgcgga tccgggcgct gctggcccgg agcgggttgg gcggaccccg
     8641 agccggctcg gtgcaggcga ccctccgcag ggtcatcgtg ttctacggag ccaagggcgg
     8701 ggtcggcaac acgacgctcg cgatcaacac cgccatcgcg ctcgcgtccg agcacaagcg
     8761 acgggttgcg ctgatcgacg cgaacctgca gttcggagac ctgcgcgtgt tcctcgacct
     8821 gaagctggac acggcctcga tcgtgaatgc catctcggag cccgagctgg acaccgatct
     8881 cctgcggcgc ctgatggtgt cgcactcgtc cggcatcgac ctgttgctgg ctccggcggt
     8941 tcccgagcag gccgacgtcg tgatggatag ccatcgggcg tcgcccgagg cgctgaccca
     9001 tatcatcgag ctcatgcggg ggatgtacga ctacaccgtc atcgatgcgg cccgcagcct
     9061 ggatcacgtg acgatccacc tgttcgacga ggcggatgtc gtctacgtcg tgctcaccgc
     9121 cgacctgtcg tgcctgaaga acgtccagct cgtgctcaag acgatgaccg agctgggcta
     9181 tccgcgcgac aagatccagc tcgtcctgaa ccgctcaaac gcgttcacgg ggatcaacat
     9241 cgccaacgcc gagagcgtgc tggggcgacc catcgggcac aagctcatca acgagtaccg
     9301 gggcgccatc ggtgcgctga actcgggcgc cccgttcatg atcagccgac cggggagccc
     9361 gctggccaag gccgtgagcg acttcgccgt atcggtggac aaggagctct cggtcgtcga
     9421 atccgaggct tgacaacctc ggcctaagcg gcccgtaacc ctccgacccg agactcggcc
     9481 cgccggtcgc cgccggatca gggaggcgag caggcggcgc cggcaaaccc tcttctcgac
     9541 cgtgagcgcc tccccgaggt ggaccgtgct ggccgagacg atgagcgcat ccatacgtgg
     9601 ggtcgaagct gtccccgtcc gcgtcgaggt cgacgtcgcc ttcggcctgc cgggcctgac
     9661 catcgtgggc ctggccggaa gcgccattca agaggctcgg gagcgcgtgc gaagcgcggt
     9721 acggaacagc gggttcgagg tgcctgcccg tcggataacg gtcaacctgg ccccggccga
     9781 cctgccgaag gagggcaccg cgtacgacct cgcgatcggg gtcgcgatgc tcgccgcttc
     9841 gggccagctc gaggccgatc tggcccacac cgccctgctg ggcgagctgg cgctggacgg
     9901 gtcgctgcgg ccggtggcgg gtaccctggc cctggtggcg gccgcccgag atgcgggtca
     9961 ccgggctgcg gtcgtcgccc agggcgatgt cgtcgaggcc gcggcagtgc ccgggatcgc
    10021 ggtccacggc gcgcgttctc tgggcgacgc ggtcgcccac ctggccggcg tgcggccgct
    10081 ggccgcggcc acgcggacgc ccctgccgcc ctggtccctt cccgaggcca ccgtcgatct
    10141 gcgcgacgtc atcggccagg gcgtgggccg ccgcgcgctc gagattgcgc tggccggtgg
    10201 ccacaacctc gccctcgtgg gcccaccggg cgtgggcaag accctgctcc tgtcgtccgt
    10261 gccgggcctc ctgccgccgc tcgacgatga cgaggccatc gaggtcagcc ggatttactc
    10321 gatcgccggc ctcgtcgacc gccaggtccc gttgacccgg gttcgtcccg tccgagcgcc
    10381 ccaccacacg acctcgactc aggcgctggt cggcggtgga gagcggatcc ggcccggcga
    10441 ggcgagcctg gcccatcgcg gggcgctggt cctggacgag ctcctccagt tccgaacgga
    10501 ttcgctcgac gccctccggc agccgctgga gtctgggcag gtgacgatcg cccgggtcga
    10561 aggcgcgctg cgcctgccgg cgcgcttcat gctgctggcg gccttcaacc cctgcccctg
    10621 cgggtggctc aacgcccggt cgcgggcctg cgcctgtgac ccggcgtcgg tccaacgata
    10681 cgaccgccgc ctgtcgggtc cactgcgcga tcgcatcgac ctgtgggtga tgatggagga
    10741 accgcgacgg gtggtgaccg atgccgaagc cgaggacagc gcggtcgtgg catcccggat
    10801 cggcgttgcc tgggatcgac agcacgatcg gcagggtgcc ccgaacggca ccctgggacc
    10861 ggacgcgctg ggcgccgagc gcggcttcgg gccggacgcc attcgcagcc tgacccggct
    10921 cggccgggtc cttcggctct cgccgcgtcg ggcgcaccgg gccgcgtggg tggcgcggac
    10981 gatcgccgac ctccagggga gccccggcgt atcacctgtt cacgtcgaag aggccctcca
    11041 ctaccgccca ccggtcccgg cgtgagcgtc gccgaggccg ttggccgcct tggagcgtgg
    11101 accggcctgc ccctcgacca gctcgatccg gagcgcgagc ggccgtactg gatcgcgacg
    11161 tcgcagctgc ccggcatcgg cccggtcgcg atcgcgcgga tgctggcccg agccggcagc
    11221 ctgcgcgccg catggcaggg cgggctcgtc gagcaggccg cggccgagct cccggccgac
    11281 gtgcgcgacc tggccgcgca acatgtccga ttccttcgcc agcgcggcgt cccgcgcgcg
    11341 gctgccgagc tggtggcccg cggccgcccg catggcatcc ggccgctcac cgcgctcgac
    11401 gaggactacc cgccatccct ccgccacctc gacccgcggc ccgccgtcct gtgggccgcg
    11461 ggggacccga ccgcctggca gaccacgacc gtggccgtcg tcggcacccg gcggccgagc
    11521 ggctatggcc gagccgcggc ggaggagatc ggggacgagc ttgcccgggc gggcgtgacc
    11581 gtgatcagtg gtctcgcggt cggggtcgat gcggtggcgc acaccgcggc ggtggcggcc
    11641 ggaggtcgga gcgtcgccgt cctcccgtcc ccgatcgacc ggatctaccc gcccgccaac
    11701 cgcgacctgg cgcaccgcct gcgcgacgcc ggggcgctcc tgaccgaggt tccgccgggg
    11761 cgccggattg ggaggccgga cttcgcgcga cggaatcgga tcatcgccgg catggcagag
    11821 gcggtcgtcg tcgttgaggc accggatcgg tccggggccc tgttaaccgc cgcggcggcc
    11881 atcgagctgg gccgcgagct gttcgcggtg ccgggaccga tggacgcggt gacctcccgc
    11941 ggcacgaacc ggctgatcgc cgaccacgcg gcgaccctgg tcacctcgcc ggccgccctg
    12001 cttcacctca tcggtctccg tcggtccggg ttcacggtga gcgttcgcac cctgtcagag
    12061 gccgaagggc tggtccttgg caggctcctg cagcgatcag ggtccatcga agagctggca
    12121 gatcggacgg cgctccccac ctccatgctg gcggccgcct tgaccatgct cgaggcccgt
    12181 ggactggtcg cggcatacgc gggcgccacc ttccatccga cgctcgccgc ccgccggagc
    12241 ggatggccga aagggtcggc caggacggcc tccgcgctcg ccgggagcgc gtgatgggcg
    12301 tcccgggcta tactttgcgc tccgcctacg acccccggac cttgagtaag gctgcccgct
    12361 ggtccgcagg agcatgtgtc tattgatctc tccgtcgact cgagtcgtgg gggaagtccg
    12421 ccgactggcc gctcacgtcg agccgttccg tcgccatcgg gcgatcaccc atctcaccat
    12481 cgccgtcacc gccctcgcgg tggcggtcgc cgttgccatc cctgctgcgc cggtgatcca
    12541 ggccggcctg cagacgacgc cccgcacggg ctcgatcctg cccgccaacg tggggatcgg
    12601 cgtcgccggg actgacgcgg tgatcctcca cttccccggt cccatggacc aggcggcggt
    12661 ggccaccacg atcggcctgt cgcccgcgac gaagttcagc ctgctgtggg cgggcgactc
    12721 gacctcggtt gccgtcgtcc cgtcaccgcg ctgggcggtc gacgagcggt acgtcatcca
    12781 cctgcccgcg ggaacagcga tggccgacgg cggcgccctg gccgccgatt ggcgcgccgc
    12841 attcacgacc cagaccgccc cctcggtgac ccacctgacg gtgggcgggg tcactggcac
    12901 accggccggc gacacgccga tcatcctcca ggaggtgatg tcctcgaccg gggcggcagg
    12961 caccgccccg gaggacgcgg ccaccgacga tcgcgacagc cccgacgtca cggcggacgc
    13021 gccgatcggg atcacgttca gtggcgcgat ggacgccgag acgacggagg ccgccttcca
    13081 catcgccccg gacgtttccg gctccctccg ctgggagggg accacgatgt ggttccagcc
    13141 cgaccagcgg ctcgcggccg gcacgcggta caccatttcg ctggtcggcg cccgcgatgt
    13201 cgacggcaac ccgctcggcg gtgacgtcag cctcagcttc acgacccgtg ttcgcgccga
    13261 ggcgatcaag gtcgccccgg cccttggcgc tcgcgacgta cccgccacca ccagcgtccg
    13321 gatcgtcttc acccagccaa tggccaccgc acggactgcg gcggccttca ccgttaccga
    13381 caccgccacc gggcgggcgg tggtcggtcg tccggcgtgg tcggccgatg gccggaccat
    13441 gaccttcgtg cccgcgacct cgctcggtgc gggccacacg ttcgccgccg ccctcggatc
    13501 aggagcccac gacgcggatg gcaacccggt gaccatcgcc tggcggttca cgacggccgg
    13561 acaggttgtc cagtcgtact cctcatcgtc ggtcccggtc gcggccggca gcgcgagcat
    13621 ggtccagtac gcgctgaacc agatcaacgc ggcccgcgcg tcgtatgggc tggggccgct
    13681 ggtcgtggac tccaccatca ccgcggtgtc ctacggccac gccgccgaca tgctggccta
    13741 caactacttc agccacagct cccgcaacgg cgcgacctac aagcagcgtc tgagcgcggg
    13801 tggggtgggg tatgggtact ccggcgagaa catctgttac ctcggttatg gcggcggagt
    13861 ccaggccacc ctcaactggt gccatgccca gttctgggca gagcccttcc ccggcggcgg
    13921 aaaccacaag gacaacatcc tcggcagcca cttccggcgg gttggcatcg ggatcgcggt
    13981 ggggggcaac aaggtctacg tcgtctggga ctacaccgac tgaccgtcca gcgccggtaa
    14041 gcggaccgta ccggcgccgc ggaaagccgg tgctacactc ccgcaccgat gtcgggaccc
    14101 cgcaacacgg gcggcgacct tgtcatcgtc gagtcgccgg ccaaggcgaa gacgatcgag
    14161 cgctacctcg gtgatggttt tcacgtgctc gcctcctacg ggcacgtccg cgacctgccc
    14221 aaacgcgggt tgggcgtgga cctcgagcac gacttcgcgc cacgctacga gccgctccct
    14281 gaccgcaagg acgagctcaa gcggctgacc gatgccgcga agacggcgaa catggtgtgg
    14341 ctcgccaccg acctcgatcg ggaaggcgag tcgatcgcct ggcacatcgc cgagcacgcc
    14401 ggcctggcgg agaatcggat ccggcgggtg accttcagcg agatcaccaa gcccgccatc
    14461 gaggccgcct tcgccagccc gcgcgatctg aatctcgacc tcgtcgacgc acagcaggcg
    14521 cggcgcatca tcgaccggct ggtcggctac aagctgagtc ccctggtcgc gagcaagatc
    14581 cgtcgcggcc tgaccgccgg ccgggtgcag agcgtggccg tccggctggt cgtggaccgg
    14641 gagcgcgaga tccaggcctt caccgccgtc gagtactgga cgattgctgc cgacctccgt
    14701 cgcggcgagt cgggggccgt attccgggtt gggctgacga agatcgacgg gcagaaggcg
    14761 gaggtcgggt ccgaggagga agcgaccgcc cacgtcgccg ccctgcgcga ggccagctat
    14821 cgggtcaccg atgtcacccg cggcgaacgt cgtcagaacg caccgccgcc gttcacgacc
    14881 agcacgcttc agcaggaggc gtcgcgcaag ctcgggttct ccgcgcgccg cacgatggcc
    14941 gccgcgcagg ccctgtacga gggcatctcc ctgccagggg gccaggtggg gctcatcacc
    15001 tacatgcgca ccgactcggt gtcgatcgcg gccagcgcgc tgaccgaggc gcgcgaggtc
    15061 atcgccgccc gctacggggt cgcgtcggtt cccgagcggc cgaatacgtt ccgcacccgc
    15121 tctcgcggcg cccaggaggc gcacgaggcc gtccgtccat cgagcttcgc ccgcacccct
    15181 gaatcggtgg aaggggcact gaagccggac cacgcccgcc tctacgacct catctggcgc
    15241 cgggcgatgg ccagccagat ggcgcccgcg gtcttcgacc aggtcggcgt ggacgtcgag
    15301 gccggccgtt acctgctcca cgccggtgcc cgcaagcggg tcttcgatgg ctaccaggct
    15361 gtgtacatcg agggcaggga cgaggaagaa gaggccggcg cgcccaagct gccggacctc
    15421 gcggtcggcg aggagctgga tctcgtcgaa ctggtgtccg accagcactt caccgagccg
    15481 ccgccccggt tcagcgaggc caccctgatc cgggcgctgg aggagcacgg cattggtcgc
    15541 ccatcgacct acgcgccgac gatggaggtc attcgggccc gcggctatgt cgagatgacc
    15601 gaccggcgcc tccacccgac cgaggaggcg ttcctgctga ccgacatgct gaccgggcac
    15661 ttcgccgacg tcgtggaccc cgagttcacg gcccggatgg agacccagct ggacgaggtg
    15721 gccggtggcc ggcgcgagtg ggtgcccatg gttcgcgagt tctgggagcc gttttcggag
    15781 caggtcgcgc ggggcaagga ggagatcccc aagaacgtcg aggagaccga catcatgtgc
    15841 ccggtatccg gacacccgat gctcaagcgt ctcggccgaa acggctggtt cctcggctgc
    15901 agcggctatc ccgagtgcaa gcacacgatg ccgctgccgg gcgaggagga tccggccctc
    15961 gacctgcccg gggtgggcga gacctgtccc gagtgcggcg agggcacgct gaccgggaag
    16021 cggagccggt ttggcccgtt cctcggttgc agccgctatc ccgactgcaa gtacatccac
    16081 cggccggagc ccgatccggc tgcggcggga gccgacggcg cggcaggccc cgcgcttgag
    16141 ggcgtggggg agacgtgccc cgagtgcaac caggggattc tcgccgcgaa acggagccga
    16201 ttcggtccgt tcgtggggtg cagccgctat cccgactgca agtacatccg caagtcacgc
    16261 tcggggagcc cgcgcgccgg ctccggccgc cgaaaggggg ccaagcgcgg ttcgaggcgt
    16321 cgagcctccc ctcgtgcctg accgagcgtc cacccccgac acgctcgagt ccgccgtcga
    16381 acgcttcctc tcgcacctcg ccacccgcaa cgcggcggcc gggacgctgg tcgagtatcg
    16441 gcgccacgtc accgaattcg gccgcttcct cgccggccga ggggtggact ggcgcacccc
    16501 ggaccgcgcc atcatcaggg cgtggctggc gaccctcgcc gagcgcgagc tgtccgcctc
    16561 ggcggtggga tcgcgactca gcgcgacccg gtcgttctat cggcacgcgg tgcggcaggg
    16621 gtggatcgac accgacccgt tgactggtgt ccgctcgcct cgtcgcccgc ggcggcttcc
    16681 gcgggtgctc agcgtggacg aggccgccag gctggtcgag gcaccagcgg cccggtcgga
    16741 aaccgcctcc gccaccggac ccgcgcccgc ccgtcgcacg ccacccgacc tcgaagacgc
    16801 cctgcgccgc cgggatgccg cgatcctcga gctcctgtac gcgacgggga tgcgaatcag
    16861 cgagctggcg ggcctctcgc tgaccgacgt cgatctcggc cgtcggcggc tccgtgtcct
    16921 ggggaagggc cgcaaggagc gcgacctgat cttcggccga ccggccgcca ccacgctcgg
    16981 cgcctacctc ggcggcgcac gaccattcct ggccgcccgc ggccacggat cggggcccga
    17041 cgccgtcttc ctgaacgcga atggcggacg gctgaccgcc cgcggcgccc gcctggtcgt
    17101 gtcccgatgg gtgcgggtcg ccgagctcgg cggccgaacc tcgccgcaca ccctgcgtca
    17161 ctcctttgcc acccacctgc tcgaaggcgg tgccgacctg cgcaccgtcc aggagctgct
    17221 cgggcattcc tcggcacaga ccacccagat ctacacccac ctgtccgacg cggccctccg
    17281 ctcggcgtac cgatcggctc acccgcggtc cgcccgcccg tcccgcacgc gcacctgacg
    17341 tgaccacccc ccgcccgccg gacctgtctt cggctgccgg cgtcgtcgtc cgcgccagcc
    17401 tggtcatgac cgccgccgcg gtggtgagcc gactcctggg ctggatccgg ctgctggtca
    17461 tcggcgccga gttcggcgcg acgtccgagc tcgacgccta cctcgcggcg ttccgcatcc
    17521 ccgatgcgat cttccagctc gtcgtggccg gcgccctgtc agcggcgctc atcccggtct
    17581 acgccgggta tcgggcgcgc ggcgaggagg acgagggatg ggcacttgcg agcagcgtca
    17641 tcaacctcgt gctggtggcc ctggtagtgg ccagcggagt catggccctc gcggcgccgt
    17701 ggctcgtccc gatcatcgcg cccggattcg acgcggacac gaccgcgctc accgttcagc
    17761 tgacccagat catgctgctc agcccggtgt tcatcgggct tggtgccgtc atcagcggtc
    17821 tcctccaggc gcacggcacc tttggcccca ccgcgctcgc gccgctcgtc tataacctcg
    17881 ccatcatcgg cgccgcggtg ttcctggcgc cgagcctcgg gatcgaggcg ctggccgccg
    17941 gcgtggtcgt gggagcgatc ggccatctcc tcgtccagct ccctgccctg cgccggctcg
    18001 gaccgcgatg gcggccgcgg atcgacctcc gccatcgggg cgtgcgccgg gtcacggtgc
    18061 tcatgggacc gcgcttcctg ggcctggcgg cggggcagat caatctcatc gtcagcacgg
    18121 cgctcgcctc cagcctggcg gcgggcagca tcaccgcatt cacgtatgcc ttccagctga
    18181 gccagctgcc ggtcgggatc atcggggtgt cgatcgcggt agccctgttc ccaaccctgt
    18241 cccgcgatgc cgcccttggt cgcgcctccg agatccggcg ccaggtgaac gccagcatgc
    18301 gggtcctgtt cttcgtatcg gccccgctcg cggtgctgat ggtcatcctc cgcgagccgc
    18361 tggcctcggt cttcttcgag tacggcctgt tcgacgccga gtccgctgaa cgaatcgcct
    18421 ccgccctggc ctggttcgcg atcggcgtcc cggcccatgt cgtggtccac gtcctggccc
    18481 gcgcgttcta tgcgatgcag gacacctgga cgcccgtggt gtgggccgtc atcgcggtca
    18541 tatcgaacat cgtcctgatg ctcgtcctgg tcgacccgat gggagtcgag ggcctggccc
    18601 tggccctgtc ggtcagcgcg acgctcgagg tcatcggcct gttgatcgcc cttcgtcggc
    18661 ggctgggcac gctcgacggg cggttcctgc tggcctcggt gacacgctcg agcgtggccg
    18721 ccgtgctcgc cggcatcgcg atgttcatcg ccctcggctt catgaacgtc gccctcggcg
    18781 gggcctcgtt cggccggctg ctgaccctgc tcgtgccggc caccgtcggc ggactggtat
    18841 tcctgggcac ggccctggtc ctccgcgctc ccgagctggc gacgctccgt cgcctcgtcc
    18901 gacgaacccc gcgatgacct tccgacccgc cgtcgacgcc gaccgggacg gctggcaggc
    18961 cctcgtcggc cgccatccgt cgggcgactt cctgcacgac tggggctggg ccgaggtcgc
    19021 ggcccacgat gggcagcccc agcgacggtt cctgctcgaa gaggtcgggg agccggtggc
    19081 cgtggttgcc gcccaggtcc gtccgatcgg gatgagtcgg tccttctggt acgtcccaca
    19141 cggtccggtc accgacttca cggctccgac cgccgcggac cgggtggaga ccctcgtcgc
    19201 cggcctggcc gccgaggccc gcgacgaccg cgccgtggcc cttcggctcg agccgcggat
    19261 ccccgccgac gccgcggagg cgacgctgct cgacgccgtg gcgcatcggg tgtcggagcg
    19321 cctgcaggtg ggtcagacca ggatcgtgga tctcgtcgag gacgacgcgc tgctgagcgg
    19381 gttcgactcc gacacccgat atggcgtgcg ccgggctgag cgcgagtcgg tggccatcac
    19441 cctggtcacc gacccggccg acatggacgc catcgagcgc ctccacgagc tcgtccgcga
    19501 aacccagcag cgggccgggt tccgcctgcc gccgatcgag cgctaccggg tcgcctggca
    19561 ggccctggcc ggcgcgggtc gcgcctgcct ggtggaggcc cgccgcgagg gcgagctgtt
    19621 ggccaccggc atgctcgtgg tcgagggcgg acagtcgttc tacctcttcg ccggctcgcg
    19681 ccgcgaggcg tccggggagc cgaagcggta tgcctcgtac gccgtccaat gggccatgat
    19741 gcgcgaggcc cgccgccgcg gcgcgacccg ccacgacctc tggggcgtcg cgccggcgaa
    19801 cgccggtccg gaccacccat ggcacggcgt cgggctgttc aagaagggat tcggtgggca
    19861 cgaggtgaca tgggccggca gctgggagat cgtcatcgac ccgctcgtct accgcctgcg
    19921 cgccgcggca tcccgcctca ggcgccgaac gccgagagac gtcccgtgag cgaacggacg
    19981 atgggactcg gggcgctcgc cgatgcggtg aacccggacc gcgtggtggg gatcccggtc
    20041 ggggaggtca ccgcgctcac tgcccagagc gccgaggcga cgcccgggac ggcattcttc
    20101 gcgattcccg gccaggttca cgatgggtgg gcgttcatcc ccgaagccgt ggccaacggg
    20161 gccgtcgcgg tcattgccga acgggagacg cccggcctgg tcgtgcccca gatcctggtg
    20221 gccgacgccc gccatgcggt ggccgacgcg gccgacgcct ggttcgggcg gccatccgag
    20281 cgtctccggg tgctgggcat caccggcact gacgggaagt cgacgaccgc gttcctcctg
    20341 ctcgacgtcc tgttggctgc cggtcggcgg ccgggcatgg tcgggaccat cgacacccgg
    20401 atcggcgaca cccagaccgc atcgaccagc cggacgacga cgcccgaggc cctcgagctc
    20461 caggcgctgc tggcggccat ggtcgaggag ggcaatgact cggtcgtgat cgaggccacc
    20521 tcccatggcc tcgccca
//
LOCUS       DASYGU010000287         4152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_356847, whole genome shotgun sequence.
ACCESSION   DASYGU010000287 DASYGU010000000
VERSION     DASYGU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356847"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VGO32_08305"
     CDS             <1..246
                     /locus_tag="VGO32_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV7200787.1"
                     /translation="ALAGTIGFIGLVVPHLVRLALGPDHRLLLPVSCLGGAALLVTAD
                     LVARLAGGVPVGVITALIGAPFFLWLLHRASAAATSP"
     gene            243..1049
                     /locus_tag="VGO32_08310"
     CDS             243..1049
                     /locus_tag="VGO32_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200788.1"
                     /translation="MSLPSSMPVPLLALDEVTFAYRRRGRPDRRVLHGISLSVAPGEL
                     VFLLGPNGSGKTTLLRVAQGMLPAGHGAVVVEGRPVGDWTRRELAQRMALLPQLATLP
                     DGFRVEELVEMGRSPHARSRFGASDQDATAVERALVDADALDLVGRPIDELSGGERQR
                     VLVAMALAQEPRLLLLDEPTQHLDLAHQVSLLTTLDRLRRTRNLAVLAVLHDPALTAL
                     ADPRVVLLSEGRIVADGPAADVLEPATIARVFGVPLPTVLALSGGRGARP"
     gene            complement(1046..2215)
                     /locus_tag="VGO32_08315"
     CDS             complement(1046..2215)
                     /locus_tag="VGO32_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7200789.1"
                     /translation="MIELFQGAWVRERRLLALFLITGSIYFALLALGMVLNVLTQFQD
                     IIVAVFLAWLLAFLISPLVHRVQERFHIGRGAAVGVSFGTTLVMLGGFLLVVAAVMVA
                     DLADFITTWPSRQDQITAQIEEIQDAIGIQEPDVAVLFDQLADQIGALGNDLAGSAGA
                     IAGGAFGVIGTLFLVVLLALFMVLDSHEIMVWMSRLVPARLRDQSILFQEGVARSFGG
                     FIRTQTLLALIMFTLVMLVGLVARLFGGMEYIFVTAVVSGLLMFIPMIGPPLALIPPI
                     FIAFLTSSNWLTALVASVVLVLVQSVLVNWLQPKMMKESLGLHPILLFLGLLVGVQIA
                     GLWGALFGVPILAVIVILVNHWLDIYSPPDPQFVAEVEAAHVDVAPATPDTGSAD"
     gene            complement(2212..3216)
                     /gene="trpS"
                     /locus_tag="VGO32_08320"
     CDS             complement(2212..3216)
                     /gene="trpS"
                     /locus_tag="VGO32_08320"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV7200790.1"
                     /translation="MSAAANRERVFSGIQPSGASHLGNYLGAQRNYVALQDEYDAIYA
                     IVDYHALTTVHDGAELRQLTRDMALDLLAVGLDPDRCALIRQSDFPEVAELCWIFSTV
                     VPVSWLQRNPTYKEGLAEGKDNSNGLLSYPVLQAADIVIYKASRVPVGKDQAPHLELS
                     REIVRAFNNRYGPIFPEPTAVFTDAPVVLGTDGARKMGKSAGNTIPIFAEPDEIRRIV
                     MNMVTDPQRIKRTDPGRPEVCNVCQLHRYFGGDYLEIQDGERTARTGCVDTKQLLAER
                     MIEFFRPMREKRAALAADPDVVEAVLSAGAEKVRPILEATRREVLEAVGTGGPGSRAT
                     "
     gene            3300..4130
                     /locus_tag="VGO32_08325"
     CDS             3300..4130
                     /locus_tag="VGO32_08325"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEV7200791.1"
                     /translation="MSDPRSQGSESAPDWGAAARPGRRVGGSVEFHAEIGSTNDRARQ
                     AFEEPHGDGLAVVADRQLAGRGRMGRTWLSPAGVNLLVSVGLRLDLPADRAWWLSAAA
                     ALALRDAALAATGGASPLAVRWPNDLVTADGLKVAGLLIETQIAGERLTAAVLGMGLN
                     VNWLRSSMPADIAPTATSMADLAGTPIDRVALLGELLDALDREVLLVEAGTTPQARFQ
                     AASWLTGRSVEVETPAGRLTGVAGAIAENGGLVLDGPTIQTTVNVGEVIRVHAAAGSR
                     "
BASE COUNT          624 a         1443 c         1453 g          632 t
ORIGIN      
        1 gcgctggcag gcaccatcgg gttcatcggt ctcgtcgtcc cgcacctcgt ccggctcgcg
       61 ctggggccgg accaccggct cctcctgccg gtcagctgcc tgggcggcgc cgcactgctg
      121 gtgaccgccg acctcgtcgc ccggcttgcc ggtggggtgc cggtcggggt catcaccgcg
      181 ctcatcgggg cgccgttctt cctgtggctc ctccatcgag cgtcggccgc ggcgacctcg
      241 ccatgagcct tccgtcctcc atgcccgtcc cgctgctcgc cctcgacgag gtgacattcg
      301 cgtatcgccg tcggggtcga ccggatcggc gggttctgca tgggatcagc ctgagcgttg
      361 cgcccggcga gctcgtattc ctcctcggcc cgaatgggag cggcaagacg accctgctcc
      421 gcgtcgccca gggcatgctc ccggctgggc acggagcggt cgtcgtggag gggcgcccgg
      481 taggcgactg gacgcgccgc gagctggccc agcggatggc gcttctgccc cagctggcga
      541 cgctgcccga cggcttccga gtggaggagc tggtcgagat gggacggtcg ccccacgccc
      601 gctcacggtt tggcgcgagt gaccaggacg cgaccgcggt cgaacgggcg ctcgtcgatg
      661 ccgacgccct ggacctcgtc ggccggccga tcgacgagct ctccggcggt gagcgccagc
      721 gggtgctggt ggccatggcc cttgcccagg agccgcgcct gctgctgctc gacgagccga
      781 cccagcacct cgatctcgcc catcaggtgt ccctcctgac caccctcgat cgcctccgac
      841 gaacacgcaa cctcgcggtc ctcgcggtcc tccacgaccc ggccctgacc gctctggccg
      901 atccgcgggt cgtcctgctc agcgaaggac gaatcgtggc cgacggcccg gcggctgacg
      961 tgctggagcc ggccaccatc gcccgggtgt tcggtgtgcc cctgccgaca gtcctcgccc
     1021 tgtccggagg acgaggcgct cgaccctagt ccgcgctccc ggtgtcgggg gtggccggcg
     1081 ccacgtcgac gtgggcggcc tcaacctcgg ccacgaactg cgggtccggc ggcgagtaga
     1141 tgtcgagcca gtgattcacc aggatcacga tgacggccag gatgggcacg ccgaacagcg
     1201 cgccccacag gcccgcgatc tggaccccca ccagcaggcc gaggaacagg aggatgggat
     1261 gcagcccgag agactccttc atcatcttcg gctgcagcca gttcacgagc acgctctgga
     1321 cgaggaccag gacgactgat gcgacgagcg cggtcagcca attcgagctg gtcaggaacg
     1381 cgatgaagat gggcgggatg agcgcaagcg gcggtccgat catgggaatg aacatcagga
     1441 ggccggacac gaccgcggtg acgaagatgt actccatccc gccgaacagg cgcgcgacca
     1501 ggccgaccag catgaccagc gtgaacatga tcagcgcgag gagggtctgg gtccggataa
     1561 acccgccgaa cgagcgtgcg accccctcct ggaacaggat cgactggtcg cgcaggcggg
     1621 cggggaccaa ccggctcatc cagaccatga tctcgtggct gtcgaggacc atgaacaggg
     1681 ccagcaggac gaccaggaac agggtgccga tgaccccgaa tgcgcctccg gcgatcgcac
     1741 ccgccgaccc cgcgagatcg ttgccgagcg cgccgatctg gtccgccagc tgatcgaaga
     1801 ggaccgccac atcgggctcc tggatcccga tcgcgtcctg gatctcctcg atctgggccg
     1861 tgatctggtc ctggcgggac ggccaggtgg tgatgaagtc ggcgaggtcg gcgaccatca
     1921 ccgcggcgac gacgaggagg aagccgccaa gcatgacgag ggtggtcccg aacgagacgc
     1981 ccaccgccgc gccgcgcccg atgtggaacc gttcctgcac ccgatggacg aggggggaga
     2041 tgaggaacgc gaggagccag gccaggaaga ccgcgacgat gatgtcctgg aactgggtca
     2101 ggacgttgag caccatcccg agcgccagca gcgcgaagta gatcgacccg gtgatcagga
     2161 acagcgcgag gaggcgccgc tcgcggaccc acgccccctg gaacagctcg atcacgtcgc
     2221 gcgcgacccc ggcccgccgg ttcccactgc ctccagcacc tcccgtcgcg tggcctcgag
     2281 gatcggtctg accttctcgg ctccggccga gagtaccgcc tccaccacgt cagggtcagc
     2341 ggccaatgcg gcgcgctttt cacgcatcgg ccggaagaac tcgatcatcc gttccgcgag
     2401 cagctgcttg gtatccacgc agccggttcg cgcggtccgt tcgccatcct ggatctcgag
     2461 gtagtcgccg ccgaagtacc gatggagctg gcagacgttg cagacctccg gccggcccgg
     2521 atcggtccgc ttgatgcgct gcgggtcggt gaccatgttc atcacgatcc gacggatctc
     2581 gtccggctcc gcgaagatcg ggatcgtgtt cccggccgat ttgcccatct tccgggcccc
     2641 gtccgtgccg agcacgaccg gcgcgtcggt gaagacggcg gtgggctcgg ggaagatcgg
     2701 tccgtatcgg ttgttgaacg cgcgcacgat ctcccgagac agctcgaggt gcggcgcctg
     2761 gtccttgccc accggcaccc ggctggcctt gtagatcacg atgtccgccg cctgcaggac
     2821 cgggtaggag agcaggccgt tgctgttgtc cttgccttcg gccagccctt ccttgtaggt
     2881 cgggttccgt tgcagccagc tgaccgggac caccgtgctg aagatccagc acagctcggc
     2941 gacctcggga aagtcggact gccggatgag cgcgcatcgg tccgggtcga ggccgaccgc
     3001 cagcaggtcg agtgccatgt cccgggtcag ctggcggagc tcggcgccgt cgtggacggt
     3061 cgtcagggcg tggtagtcga cgatcgcgta gatcgcgtcg tactcgtcct gcagcgcgac
     3121 gtagttccgc tgcgcgccga ggtagttgcc gagatgactc gcgccgctgg gctggatgcc
     3181 cgagaacacc cgttcgcggt tggcggcagc gctcatggtc cgggcgagtg taccggaccc
     3241 gattttgacg gctggtagaa tcgcccccct tcccgaccga ccgtcatacg aggctatccg
     3301 tgtccgatcc acgctcgcag ggctccgaat cagcccccga ctggggtgcg gcggcgcgcc
     3361 cgggacggcg ggtcggcggg tcggtcgagt tccacgccga gatcggctca accaacgatc
     3421 gtgcgcgcca ggcgttcgag gagccgcacg gtgatgggtt ggcggtcgtc gccgaccggc
     3481 agctggcggg tcgtggccgg atgggccgca catggctgtc gccggccggc gtcaacctcc
     3541 tggtcagcgt cgggcttcgc ctggacctgc ccgccgatcg ggcctggtgg ctgagtgccg
     3601 cggcggccct tgccctccgt gacgccgcgc tggcggcgac cggcggtgca tcgccactgg
     3661 ccgtgcgctg gccgaacgac ctggtcaccg ccgacggcct gaaggtggcc gggctgctga
     3721 tcgagaccca gatcgccggt gagcgcctga ccgcggccgt gctcgggatg ggcctgaacg
     3781 tgaactggct ccgctcgtcc atgcccgctg acatcgcgcc gaccgccacg tccatggcgg
     3841 acctggccgg cacgccgatc gaccgggtcg cgctcctcgg cgagctgctg gatgccctgg
     3901 accgggaggt cctgttggtc gaagcgggga caaccccgca ggcccgcttc caggccgcgt
     3961 cctggctcac cggtcgctcg gtcgaggtcg aaacgcctgc cggacggctc accggcgtgg
     4021 ccggggcgat tgccgaaaac ggcggcctgg tgctggacgg gccgaccatc cagaccacgg
     4081 tgaacgtggg cgaggtcatc cgcgttcacg cggcggcagg atcccgatga gcgagccgcg
     4141 acggcgcccc ga
//
LOCUS       DASYGU010000288         4489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_820130, whole genome shotgun sequence.
ACCESSION   DASYGU010000288 DASYGU010000000
VERSION     DASYGU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820130"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGO32_08330"
     CDS             complement(<1..491)
                     /locus_tag="VGO32_08330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7200792.1"
                     /translation="MEERTVESRVAYAGRLLTMRVDEVVMTDGRPATREIIEHPGAVG
                     ILAWDGERMAAVRQWRQAAGRETLEIPAGTRDPGEEPRQTAERELAEECGVSAGRWEA
                     GPAFWTAPGFCTEYLSLWLATDLSPVDVTSPEDEALERTWLTLDEALTAVADGAINDA
                     KSLV"
     gene            540..1502
                     /locus_tag="VGO32_08335"
     CDS             540..1502
                     /locus_tag="VGO32_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7200793.1"
                     /translation="MLVISVHGLERRFRTVTAVYDLSFNVPAGAVYGLLGPNGAGKTT
                     TIRMLAGLIAPTAGTAEIAGHPLGPDNAAIRAMTGILTESPGLHDRLTARQNLAYYGR
                     LYGLTDGPLREAVDRYLDIVGLTEAADRRVGGFSKGMRQRVAVARALLHEPRVIYLDE
                     PTSGLDPEAAKSIRALIASLREEGRSILLCTHNLDEAERLCDQIGILNGHLLAEGSPA
                     ELRRHGRPTTTRIELDGGPVDPAALAATVRALPFVTAVTSGAADLTVELAEPRQDTPD
                     LVRELIQQGARVVAVSENVASLEQVYLDLVGERADRDAEGVHAA"
     gene            1492..2283
                     /locus_tag="VGO32_08340"
     CDS             1492..2283
                     /locus_tag="VGO32_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7200794.1"
                     /translation="MRPDLVWIVFGKEWREIASNRLLLGAVAAPAIIFAAIPTAIVAF
                     IQVNDIDMAQLGQIELMLAAFPDLPVKLAAQGFIVTNFMAYFLLIPAMVPIAIASQSV
                     IGERQARSLEPQLATPIEVSELLAGKTIAAALPAILATWVVWVGYGLINAAIADPRLL
                     RMVFSPQWIVAMASLVPLICVLSVLLGVIVSSRVGDPRTAQQFGAFVVLPIIAIAVAQ
                     FLGGQATFTMQQVLTADLITIGLIGGLLVIGSWAFNREEILTRLG"
     gene            complement(2239..2886)
                     /locus_tag="VGO32_08345"
     CDS             complement(2239..2886)
                     /locus_tag="VGO32_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV7200795.1"
                     /translation="MSDDDDVVNENTPAGRIRSSSPARIYVVGTSGSGKTVLAEKLAD
                     QLGLEHVQLDALYWGPGWSESDRASFAERARAVTEQPSWVIDGAHRAAIVASWDRAEW
                     VIWLDYPLRTILRRLIRRTARRVIRQEPLHHDNVERYRRALGRRSPIVWAIRTHDDLR
                     REIEGRFERETERVLVRLRSPRETDQWLRSLAGMPSVSKPPSSEAGEDLLAIEGP"
     gene            3035..>4489
                     /locus_tag="VGO32_08350"
     CDS             3035..>4489
                     /locus_tag="VGO32_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7200796.1"
                     /translation="MATASVTSDSPSLERNAVGATGVAFQSITHMAPAVATAASLGAA
                     TVFAGAITPLAVLFAMVAALFTAYSLGELARHLPSAGGMYTYVGRGLGSFFGWLMAWA
                     FTLAEPLVAPLLFGGFGFFGAIFVATYLNVELTEMWAILAVACGLLTWWLLYSGISFS
                     TRAGVVLGSIEIAIFLVISTLLIINAGSNNTVDVFIPGADGLTPALQGMVFCLLAFIG
                     FEAAAPLGEEAKDPKRTIPRAVVWSTILIGAFYVFNMYAATVYFGPDRMASEFLGFNA
                     GDPWGGMAEEVLPVIGGFAVLFAILNSCLANASAGATAATRSVFNMGRGGLLPRFLGA
                     VHPTRQTPVNAVHVQMILGIALVLILGFVLTTDPFPAGLNVYAFLGTLLGLLFAGMYM
                     AVNLACIGYFLREGREHFNPIKHLVVPIIGFIAMIPALLFVIGGVTIPIIDLEVPSLG
                     GDLVLIAPIAGVWMLIGIVLYFVLRAMRPQALKEL"
BASE COUNT          641 a         1635 c         1404 g          809 t
ORIGIN      
        1 accagcgact tcgcgtcgtt gatcgcgccg tcggccaccg cggtcagcgc ctcatccagg
       61 gtcagccagg tgcgctcgag cgcctcatcc tccggactgg tgacgtcgac cggcgacagg
      121 tcggtggcaa gccacaggct caggtactcg gtgcagaacc cgggcgccgt ccagaacgcc
      181 ggtccggcct cccacctgcc cgcgctcacc ccgcactcct cggccagctc ccgctcggcg
      241 gtctgccggg gctcctcccc cgggtcgcgg gtcccagccg ggatctcgag cgtctcccgc
      301 cccgccgcct gccgccattg gcgcaccgcg gccatccgtt cgccgtccca cgccaggatc
      361 cccaccgcgc cggggtgctc gatgatctcc cgcgtggcgg gccgtccatc ggtcatcacc
      421 acctcgtcca cccgcatcgt gagcagccgc cccgcgtacg caacccgaga ttccaccgtt
      481 cgctcttcca tggcccgatc ttgccattcc ggaccccccg cctgcccctg ctagcatcga
      541 tgctcgtgat ctcggtccat ggtcttgagc gtcggtttcg cacggtcacc gccgtgtacg
      601 acctgtcatt caacgtgccc gcgggcgcgg tctacgggct cctcggcccg aatggcgcgg
      661 gcaagacgac caccatccgg atgctggccg ggctcatcgc gcccactgcc ggcacggccg
      721 agatcgcggg acatccgctc ggaccggaca atgccgccat ccgggccatg accgggatcc
      781 tgaccgagag ccccggcctc catgaccggc tcactgcgcg tcagaacctc gcctactacg
      841 gccgcctgta cggcctgacc gatggtccgc tgcgggaggc cgtggaccga tacctcgaca
      901 tcgtgggcct gaccgaagcc gccgaccggc gcgtcggcgg cttctccaag ggcatgcgcc
      961 agcgggtcgc cgtcgccagg gctctcctcc acgagccgcg ggtcatctac ctcgatgagc
     1021 ccaccagcgg actcgacccg gaggccgcca agtccatccg cgcgctgatc gccagcctcc
     1081 gcgaggaggg ccgctcgatc ctgctgtgca cccacaacct cgacgaggcc gagcgtctgt
     1141 gcgaccagat cggcatcctg aacggccatc tcctggccga gggctccccg gccgagctcc
     1201 ggcgccacgg gcgcccgacg acgaccagga tcgagttgga cgggggaccg gtggaccccg
     1261 ccgccctcgc cgccacagtg cgcgcgctgc cgttcgtcac cgcggtcacc tccggcgcgg
     1321 cagatctcac cgtggagctg gccgagcccc gccaggacac cccggacctc gtcagggagc
     1381 tcatccaaca gggcgcgcgg gtggtggccg tcagcgagaa cgtggccagc ctggagcagg
     1441 tctacctcga cctcgtgggt gagcgcgcag accgcgatgc ggagggggtc catgcggcct
     1501 gacctggtat ggatcgtgtt tggcaaggaa tggcgagaga tcgcctccaa ccggctcctg
     1561 ctgggcgcgg tggctgcccc ggccatcatc ttcgccgcca tcccgaccgc catcgtggcg
     1621 ttcatccagg tcaacgacat cgacatggca cagctcggcc agatcgagct catgctcgcc
     1681 gcgttccctg acctgccggt caagctcgcc gcccagggct tcatcgtcac gaacttcatg
     1741 gcctatttcc tcctgattcc ggccatggtc cccatcgcca tcgcctccca gtcggtcatc
     1801 ggggagcgac aggcccggtc actggaaccc cagctcgcga ccccgatcga ggtctcggag
     1861 ctgctggccg gcaagaccat tgccgccgcc ctgcccgcca tcctggccac ctgggtggtg
     1921 tgggtcggct acggactgat caatgccgcc atcgccgacc cgcgcctcct tcggatggtg
     1981 ttcagcccgc agtggatcgt ggccatggcc agcctcgtcc cgctcatctg cgttctgtcc
     2041 gtcctgctgg gcgtgatcgt gagctcgcgg gtgggcgatc cgcgcaccgc gcaacagttc
     2101 ggcgcgttcg tcgtgctccc gatcatcgcc atcgcggtgg ctcagtttct gggcggacag
     2161 gccaccttca cgatgcagca ggtcctcact gcggacctga tcacgatcgg cttgatcggc
     2221 gggctgctgg taatcggctc atgggccttc aatcgcgagg agatcctcac ccgcctcgga
     2281 tgacggcggc ttgctgactg aaggcatgcc agcgagagat cgcagccatt gatcggtctc
     2341 tcgcggggag cggaggcgca caaggacccg ctccgtttcc cgctcgaatc gaccctcgat
     2401 ctcgcgacgg agatcatcat gggtccggat cgcccacacg atcggggagc ggcgccccaa
     2461 tgcccttcgg taccgttcca cgttgtcgtg atggagcggc tcctgccgaa tgacgcgacg
     2521 agccgtgcgg cggatgagcc gtcggaggat ggttcgcagg gggtaatcga gccagatgac
     2581 ccactcggcc cgatcccagg aggccacgat ggcggcccga tgagcgccgt cgatgaccca
     2641 tgaaggctgc tccgtcacgg cgcgggcacg ctcggcaaag ctggcccgat cggactccga
     2701 ccagcccggt ccccagtaca gcgcatcgag ctggacatgc tccagaccga gttggtccgc
     2761 gagcttctcg gcgaggaccg tcttccctga gcccgaggtc ccgacgacgt agattcgcgc
     2821 cggcgacgac gaccgaattc ggcctgcggg tgtgttttcg ttgaccacgt cgtcatcatc
     2881 gctcacccgg cgatcctgtg gaactccacc aaaccccaca aaacttcccc cttccccccg
     2941 taaccggtcc atgcctagac tgtcggcgcg tcctgccgtt tcgggtgggt tccacccctc
     3001 ttggcggccg aaggcgcaca ccggaggagg atccatggcc acagcctcag tgacgtccga
     3061 ctcgcccagc ctggagcgga acgcggtcgg tgcgaccggc gtggccttcc aatccatcac
     3121 tcacatggcg ccggcggtcg caaccgcggc ctcgctcggc gcagccaccg tctttgccgg
     3181 cgcgatcaca ccactggccg tcctgttcgc catggtcgcg gccctgttca ccgcctactc
     3241 gcttggcgag ctggcccgtc acctgccttc ggcgggaggc atgtacacct atgtcggccg
     3301 cgggctggga tccttcttcg gctggctcat ggcctgggca ttcacgttgg ccgagcccct
     3361 ggtcgcaccg ctcctgttcg ggggcttcgg gttcttcggc gcgatcttcg tcgcgaccta
     3421 cctgaacgtg gagctcaccg agatgtgggc catcctggcc gtcgcctgcg gcctgttgac
     3481 ctggtggctg ctgtacagcg gcatctcgtt cagcacccgg gccggggtcg tcctgggctc
     3541 catcgaaatc gctatcttcc tcgtcatctc caccctgctc atcatcaacg ccgggtcgaa
     3601 caacaccgtg gatgtcttca ttcctggcgc ggacggcctc acgccggccc tgcaggggat
     3661 ggtcttctgc ctgttggcct tcatcgggtt cgaagcggct gcgccattgg gcgaagaggc
     3721 gaaggatccc aagcgcacca tcccgcgggc ggtggtctgg tcgaccatcc tgattggcgc
     3781 cttctacgtg ttcaacatgt acgccgccac cgtgtacttc ggtcccgatc ggatggcgtc
     3841 cgagttcctc ggcttcaacg ccggcgatcc atggggcggg atggcggagg aggtcctgcc
     3901 ggtgattggc ggattcgcgg tcctgttcgc catcctcaac agctgcctgg ccaatgccag
     3961 cgcgggcgcg accgcggcga cccgttcggt gttcaacatg gggcgcggcg gactgctgcc
     4021 ccgctttctg ggagcggtgc acccgacccg gcagacgccg gtgaacgcgg tgcacgtcca
     4081 gatgatcctg ggcatcgcgc tcgtgctcat cctgggattc gttctcacga ccgatccctt
     4141 cccggccggc ctgaacgtct acgccttcct gggcaccttg ctcgggctgc tgttcgccgg
     4201 gatgtacatg gccgtgaacc tggcctgcat cggctacttc ctgcgtgaag gtcgggagca
     4261 cttcaacccg atcaagcacc tggtcgtccc gatcatcggc ttcatcgcga tgatcccggc
     4321 cctcctgttc gtgatcggcg gcgtgaccat cccgatcatc gacctggagg ttccttcgct
     4381 cggcggtgac ctcgtcctga tcgccccgat tgcgggggtc tggatgttga tcgggatcgt
     4441 cctgtacttc gtcctccgtg ccatgcggcc gcaggcgttg aaggagctt
//
LOCUS       DASYGU010000289         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_769172, whole genome shotgun sequence.
ACCESSION   DASYGU010000289 DASYGU010000000
VERSION     DASYGU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769172"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="VGO32_08355"
     CDS             complement(<1..1887)
                     /locus_tag="VGO32_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV7200797.1"
                     /translation="MGALAISFLLFPVLAGPVSAVRGETVPVPAPNNLTQDSWIVQLR
                     PGVSPAAQALDLSRLAGGRAGLVFQIAINGFQFRGTQAAAQMLARHPWVESVRADRPI
                     HLVESLPNGIERIWAYDNDSPADSAYHQGFRGNGARIAVLDTGIDLDHPDLVGAIDQG
                     LGKNCVNPALPPEDGYGHGTHVSGTAAAPINGVGVVGVAPESRLVPVKVFDDAGNSSE
                     ALVLCGFNHVLALNADVDPGNDVDVMSMSFGEDRAWGDCVTDALHEAVCNAYAAGIIM
                     VAGAGNSAANAGTFVPAAFPEVISVSALADFDATRGGLAGCGLVPDLGWFDCDDTFAF
                     FSNYGASVDVIAPGVSIFSTTKNGGYATQSGTSMATPHVSGVVALMAAANPGLTPAAA
                     RAFLQQSGECPNGSVAGADGACDGQGTWADDPDGTPEPMANALRAALAAGGSPPTPTP
                     PAAPTLTSATAGDASVALSWTVPADGGSLLSGYEVWRGTVSGTTALLAAPVGLQTTFT
                     DLTAANGTTYYYQVRAVNGVGPGPLSNEMSATPLAPPPTVTRIGGTVSNFASATSSSG
                     SLAFSPPAGSNALVAMVSVSHTTVTLTAVTWRPDPANPGADQALTFVGRRVAPSAGAV
                     EIWAL"
     gene            complement(2085..3242)
                     /locus_tag="VGO32_08360"
     CDS             complement(2085..3242)
                     /locus_tag="VGO32_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7200798.1"
                     /translation="MEITAPTILEIGALLLAAAGAGWLARRVHLPAVVGYLAVGLAVS
                     PFTPGWVADPEQLHILADIGVVLLLFEVGIEIDLFDFRREQRGLVWAAPLQMVITTLA
                     GVVVGLATGLGPLAGAVLGLAVAFSSSVVVLNITRSKRRRTDPPTEKALVGWAILQDV
                     SGVVLFGFLLAAISTGERPWYLSVIGLAAFIGLAWVAAWILPRALRAVHEDKDLFLVV
                     TIGAGLALAGLGGFVFQIPMALAAFIAGLAISESHESAEARRRILPFRDLFAILFFVA
                     LGSLINPARLPEALPWLAVVLGMIIAAKVAVIWVLSKITRLDAQPLQLAVGLAQIGEF
                     SFVLASVALAAGAIDVVLYTAILAAVAISIAISTVAVRLVRPRPMPAPAGN"
     gene            complement(3258..3920)
                     /locus_tag="VGO32_08365"
     CDS             complement(3258..3920)
                     /locus_tag="VGO32_08365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7200799.1"
                     /translation="MSNATLGLPDDLHDYLLTVGVREPDLLRELREETAAMPERDMQI
                     APEQGAFMALLVELIGARRCVEVGTFTGYSSTRIAMALPPDGRIVCCDISREWTDVAR
                     RYWERAGVADKIELRLGPALDTLDALLTEGLAGSFDFAFLDADKVHYPEYSDRLLALL
                     RSGGLLAIDNVFWSGRVTQPDSDDENVRAIRSLNRALAADERVTLAMVPIADGLTLAR
                     KR"
     gene            complement(3924..>4125)
                     /locus_tag="VGO32_08370"
     CDS             complement(3924..>4125)
                     /locus_tag="VGO32_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200800.1"
                     /translation="IPGIGGLVKLVVVVVGVGAMGVAFGEWWNRRRPPEVPPFGTVTP
                     AAPVAPAAASEGSASDASSSGI"
BASE COUNT          683 a         1417 c         1472 g          553 t
ORIGIN      
        1 cagggcccag atctcgaccg cgccggcgga gggcgccacc cggcggccga caaaggtcag
       61 cgcctggtcc gcgccggggt tggccggatc ggggcgccag gtcaccgcgg tgagggtgac
      121 cgtggtatgg ctgacgctga ccatggccac cagcgcgtta ctgccggccg gcgggctgaa
      181 cgcgagcgag ccgctgctcg aggtcgccga ggcgaagttg ctgaccgtac ccccgatccg
      241 ggtcaccgtc ggcggcggcg cgagcggcgt ggctgacatc tcattggaga gcggcccggg
      301 gccgaccccg ttcaccgcgc ggacctggta gtagtaggtc gtgccgttgg ccgcggtcag
      361 gtcggtgaag gtggtctgca gcccgaccgg ggccgccagg agcgcggtcg tcccgctgac
      421 cgtcccgcgc cacacctcgt agccggacag cagcgagccg ccgtccgcgg gcaccgtcca
      481 gctcagggcc acgctcgcgt caccggcggt ggcgctggtc agagtcgggg ctgcgggcgg
      541 ggtgggggtc ggcggactgc cgccagcggc gagcgcagcg cggagggcgt tggccatcgg
      601 ctccggcgtg ccgtcgggat catcggccca cgttccctgc ccgtcacagg cgccgtcggc
      661 accggccaca gaaccattcg ggcactcccc agactgctgc aggaacgcgc gcgcggcggc
      721 aggggtcagg ccagggttgg cggccgccat gagcgccacc accccgctga cgtggggggt
      781 ggccatgctc gtgccgctct gggtcgcgta gccgccgttc ttggtggtcg agaagatgga
      841 gacgccgggg gcgatgacgt cgaccgatgc tccgtagttg ctgaagaacg cgaacgtgtc
      901 gtcgcagtcg aaccagccga gatcgggaac caggccgcag ccggccaggc cgccgcgagt
      961 ggcatcgaag tccgccaggg cggagacgct gatgacctcc gggaacgcgg ccggtacgaa
     1021 cgttccagcg ttcgcggccg agttgcccgc gccggcgacc atgatgatgc ccgccgcgta
     1081 ggcgttgcag accgcctcgt ggagtgcatc ggtcacacag tcgccccagg cgcggtcctc
     1141 gccaaagctc atgctcatga cgtcgacgtc attgcccggg tcgacgtcgg cgttcagggc
     1201 caggacgtgg ttgaacccgc acaggaccag ggcctcgctc gaattgccgg cgtcgtcgaa
     1261 taccttgacc ggaaccaggc gggattctgg ggcgaccccc accaccccga ctccgttgat
     1321 gggcgcggcg gccgtgcccg acacgtgggt tccgtggcca tatccgtcct cgggcgggag
     1381 cgcggggttc acgcagttct tgccgagtcc ctgatcgatg gcgcccacca ggtccgggtg
     1441 gtccaggtca atgccggtgt cgagaacggc gatccgggcg ccgttcccgc ggaacccctg
     1501 gtgataggcg ctgtccgccg gcgagtcgtt gtcgtaggcc cagatgcgct cgatgccatt
     1561 cggcagggac tcgaccaggt gaatgggccg atccgcccgg accgattcca cccatggatg
     1621 acgggccagc atctgggcgg cggcctgcgt cccccggaac tggaacccgt tgatggcgat
     1681 ctggaagacg agacccgctc gaccaccggc gagtcgggaa aggtcgaggg cctgagccgc
     1741 cgggctgacc cccggccgca gctgaacgat ccacgagtcc tgggtcaggt tgttgggcgc
     1801 ggggaccggt acggtctcgc ctcggacggc ggacacgggc ccggccagga ccgggaacag
     1861 caggaagctg atggccagcg cacccacgag acgggagcgg gcgaccccca caggctggtg
     1921 accacggacg cgggatcgag gatgagagcg cgataaggac aagaaattgt tcccctttcg
     1981 gggattgtcc gcctcgatcg gtcgcaatgg aagaccatcg gtgctgctcg gcaccgccaa
     2041 gcggtccccg tcggaatggg accttcagcc gcgctctggc gtgttcaatt ccccgcagga
     2101 gccggcatgg ggcggggacg cacgagccgg acggcgaccg tcgagatcgc aatgctgatc
     2161 gcgaccgccg ccaggatcgc ggtgtagagg acgacgtcga tcgccccggc cgccagcgcc
     2221 accgacgcga gcacgaacga aaactcgccg atctgggcca ggccgacggc cagctggagc
     2281 ggctgcgcgt ccagccgtgt gatcttggac agcacccaga tgaccgccac cttggcggcg
     2341 atgatcatgc cgagcacgac cgccagccac ggcagcgcct ccggcagtcg cgccgggttg
     2401 atgaggctgc ccagggcgac gaagaacagg atggcgaaga ggtcacggaa gggcaggatc
     2461 cggcgccgcg cctcggccga ctcatggctc tcgctgatcg ccaggccggc gatgaacgcg
     2521 gcgagggcca tcgggatctg gaacacgaac ccgcccagcc cggccagggc caacccggcg
     2581 ccgatggtca cgaccaggaa caggtcctta tcctcgtgga ccgcccgcag ggcgcggggc
     2641 aggatccagg ccgcgaccca ggccagaccg atgaacgcgg cgaggccgat gaccgacaga
     2701 taccacggcc gctcgccggt gctgatcgcc gcgaggagga acccgaacag gaccacgccc
     2761 gagacgtcct gcaggatcgc ccaacccacg agcgccttct cggtcggcgg atccgtccgt
     2821 cggcgcttgc tgcgggtgat gttcaggacc acgacgctgg acgagaatgc gaccgcgagg
     2881 cccaggaccg cacccgccag tggaccgagt cccgtcgcca ggcccacgac cacgccggcc
     2941 agggtggtga tgaccatctg gagtggagcc gcccatacca gcccacgctg ttcgcgccgg
     3001 aagtcgaaca ggtcgatctc gatcccgacc tcgaacagga ggagcacgac cccgatgtcg
     3061 gccaggatgt ggagctgttc gggatcggca acccagcccg gcgtgaacgg tgagacggcg
     3121 aggcccaccg cgaggtagcc cacgaccgct ggaaggtgaa cccggcgggc gagccatccg
     3181 gcacccgcgg ccgcgagtag gagcgccccg atctcgagaa tggtgggggc ggtgatctcc
     3241 acggcggtcg agaccgccta ccgcttgcgg gccagggtca ggccatcggc aatcggcacc
     3301 atggccagcg tcacccgctc gtcggcggca agagcccgat tcaggctccg gatggcccgt
     3361 acgttttcgt cgtcgctgtc cggctgcgtc acgcgcccgc tccagaagac gttgtcgatg
     3421 gccagcaggc cacccgatcg gagcagggca aggagacgat cgctgtactc ggggtagtgg
     3481 actttgtcgg catcgaggaa cgcgaagtca aagctccccg ccagtccctc ggtcagcaac
     3541 gcgtccagcg tgtcaagtgc cggtcccagg cggagctcga tcttgtcggc gacccccgcg
     3601 cgctcccagt aacggcgggc gacatcggtc cattcgcggc tgatgtcgca gcacacgatc
     3661 cgcccatcgg gcggcagcgc catcgcgatt cgcgtggacg agtagccggt gaacgtgccc
     3721 acctcgacgc accgtcgtgc gccgatgagc tccaccagga gtgccatgaa cgccccctgc
     3781 tcgggcgcga tctgcatgtc acgctcgggc atggcggccg tttcctcgcg cagctcgcgg
     3841 aggaggtccg gctctcggac cccgaccgtg agcaggtagt cgtggaggtc gtcaggcagg
     3901 cccagggtcg cgttcgacat cggtcagatc ccgctcgagg acgcgtccga ggcggatccc
     3961 tccgaggccg ctgcgggcgc caccggcgcc gcgggcgtca cggtcccgaa cggaggcacc
     4021 tcgggcggac ggcgacggtt ccaccactcg ccaaaggcga cgcccatcgc tccgacgccg
     4081 acgacgacca ccaccagctt cacgagccct ccgattccag gaatc
//
LOCUS       DASYGU010000290         4169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_49511, whole genome shotgun sequence.
ACCESSION   DASYGU010000290 DASYGU010000000
VERSION     DASYGU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49511"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..992
                     /locus_tag="VGO32_08375"
     CDS             93..992
                     /locus_tag="VGO32_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7200801.1"
                     /translation="MGSPLASTTVQVRDGRSLDVYLGGDTDGARGTILYHHGSPSSGI
                     QGPGMAAACAERGLRLVSSSRAGYGSSSRREGRSVADVVEDATDLLDALDVERAWVLG
                     WSGGGPHALACVALAPNRFPGAALIGGVAPYPAEGIDWFEGMGPENVEEFQATLADPE
                     NSVRSAERDWPQWRDVTGPEIAEAFGGLIDDVDRGSLTGEFAEFVAAGSREGLREGYW
                     GWVDDDWAFTKHWGFDLSSIGVPVHVWQGGHDKMVPFNHGAWLCDHIPSACRHLHPEH
                     GHLTLAVDSLGLIFDELTAPPAP"
     gene            996..2165
                     /locus_tag="VGO32_08380"
     CDS             996..2165
                     /locus_tag="VGO32_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEV7200802.1"
                     /translation="MSRLGQLARPMIGRSPDEAHRDSTPLELLFDLVFVVAVAQVSSE
                     LHHGLFVGDIGRTVFQYVFVFGAIWWAWMGFTWFASGYDTDDVVYRLVVFVQLGGALV
                     LAAGVPAAFENSDWRIVLAGYAIMRLAQVYLWLRVARDDPPNRPGALRNAAGMSLLQV
                     GWIVLVLSTPGWFVAGFMVLFAFELAVPIWAQRASRSPWHPEHITERYGLFTIIVLGE
                     SILSASLAVASLSRAGLTLELMSILFGGLVTVLAMWWLYFELPIRERTSLRAGFAWAY
                     GHMPIWAAAAAVGAGLAVAIEQATGLGALDAVGAGYAVTIPVAIYLGGLWLIHENVRV
                     DGWREAVPVVVAIAAILIVPLTGWGVLLTGLVLSALLGYKLASHGKADIGEPAAA"
     gene            2128..2958
                     /locus_tag="VGO32_08385"
     CDS             2128..2958
                     /locus_tag="VGO32_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200803.1"
                     /translation="MGKPTSENQRRLDAAAMTARVRRAANVVGVAVIAWIVLFFLSSD
                     VVSLRADSPWSEDPADLVVSLTFLLLGVVGPVTFVRVQRDAESSVMRPRTADDVMRGL
                     YVALGTITVADATMLLAWVDSAQAGGMASIGFVLAMLLGTSIATAGVAGVVVYRAARV
                     SSSWRQSIDREERDALDDIVAFTLRIRPLRTSAVAFAGFLNGRFSPRRHRWAFATLVA
                     VGFGLGYSTWHLLVEGPAPSASASFTVLIVFASFGAAAVLAGWIAFGGYLRLVRRVSD
                     "
     gene            complement(2966..3601)
                     /locus_tag="VGO32_08390"
     CDS             complement(2966..3601)
                     /locus_tag="VGO32_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEV7200804.1"
                     /translation="MKDQMRRAIVVTADGEATVAPDLALVSLAVSGQGKELAPTRDDV
                     VLRSSAVLGALRELEIAEGDINAPDVSISPEYDYRRGQKLIGYRVARPMTIKVRDLSR
                     LGEVLDGVVTAGANEVQGAQMSASDPSAAEHQALVAAFAAAHAKAQALARAAKVALGA
                     VIRVEEEDGGMAPMPRMRAMAAMADSAGAPTEVAAGDLTVTRRVRVWFAID"
     gene            complement(3612..>4169)
                     /locus_tag="VGO32_08395"
     CDS             complement(3612..>4169)
                     /locus_tag="VGO32_08395"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7200805.1"
                     /translation="VTVDRDEHPRPDTTLEQLAGLKPAFRKDESGSVTAGNSSGINDG
                     AAAVVVMSEQRARDAGLRPMLRMRSSAVAGVDPATMGLGPIPASRLALERAGLSVGDL
                     DLVELNEAFAAQALPVMRELGLDPEKVNVNGGAIAIGHPLGASGARLVATLAHEMRRR
                     GSRYGLATMCIGVGQGIATVWETAE"
BASE COUNT          537 a         1457 c         1451 g          724 t
ORIGIN      
        1 tgggaggtcc acgtccacaa cgtcgggcgc aaccagtccg agttcatccg cgcctacggt
       61 gagcgggtcc tgccccagct caagctcggc tgatgggttc gccactggcc tccaccacgg
      121 tccaggtgcg ggatggccgc agcctggacg tctacctcgg cggcgacacg gacggcgcgc
      181 ggggcacgat cctctaccac cacggcagcc caagctccgg gatccaggga ccgggcatgg
      241 ccgcggcctg tgccgagcgc ggcctgcggc tcgtcagctc cagccgggcc ggctacggga
      301 gcagcagccg acgggaaggc cgatcggtgg ccgacgtggt cgaggacgcc accgaccttc
      361 tggacgccct ggacgtggaa cgggcctggg tgctcggctg gtccggtggc gggccccacg
      421 ccctggcctg cgtggccttg gctcccaatc gattcccggg ggcggcactg atcggcggcg
      481 tggcgcccta cccggcggag ggcatcgact ggttcgaggg catggggccg gagaacgttg
      541 aggagttcca ggccacgctc gccgacccgg agaacagtgt ccgctccgcg gagcgggact
      601 ggccgcagtg gcgggatgtc actggccccg agatcgccga agccttcggt gggcttatcg
      661 acgacgtgga ccgcggctca ctgaccgggg agttcgccga attcgtagcc gccggcagcc
      721 gcgaggggct ccgtgagggc tactggggtt gggtcgacga cgactgggcg ttcaccaaac
      781 actggggatt cgacctgtcc tcgatcggcg tgcccgtgca cgtgtggcag ggaggccacg
      841 acaagatggt gcccttcaac cacggggcct ggctgtgcga ccacatcccg tcggcctgcc
      901 gccatctcca ccccgagcat gggcacctga ccctggccgt ggattcgctg ggcctcatct
      961 tcgacgagct cacggcgccg ccggccccct gaccgatgag ccggctcggc cagctggcca
     1021 gaccgatgat cggccggtcg cccgatgagg cgcaccggga ctcgacgccg ctcgaactgc
     1081 tcttcgacct cgtgttcgtg gtggccgtcg cccaggtcag cagcgaactg caccacgggc
     1141 tgttcgtcgg cgacatcggg cgcaccgtct tccagtacgt cttcgtgttc ggggccatct
     1201 ggtgggcctg gatgggcttc acctggttcg cctcgggcta cgacaccgat gacgtcgtgt
     1261 atcggctggt cgtgttcgtc cagctcggcg gggcgctggt cctggccgcc ggtgtcccgg
     1321 ccgcgttcga gaacagcgac tggcggatcg tgctcgccgg ctacgcgatc atgcggctgg
     1381 cccaggtcta cctctggctc cgcgtcgcgc gcgacgatcc ccccaaccgc cccggcgccc
     1441 tgcgcaacgc ggcggggatg agcctcctcc aggtgggatg gatcgtcctc gtcctgagca
     1501 cccccgggtg gttcgtcgcc ggattcatgg tcctctttgc cttcgagctg gcggtgccca
     1561 tctgggccca acgcgcatcc cgatccccgt ggcacccgga gcacatcacc gagcgctacg
     1621 gactgttcac gatcatcgtc ctgggcgagt cgatcctctc ggcatcgctc gccgtggcct
     1681 ccctcagccg ggcggggctg accctcgagc tgatgagcat cctgtttggt ggcctcgtca
     1741 ccgtcctcgc catgtggtgg ctgtacttcg agctcccgat ccgcgaacgc acctcgctcc
     1801 gtgccgggtt cgcgtgggcg tacggccata tgccgatatg ggcggcggct gcggcggtcg
     1861 gcgccgggct cgcggtcgcg atcgagcagg ccaccggact gggggcgctc gatgcggtgg
     1921 gggcgggata cgcggtcacg atcccggtgg cgatctacct cggcggcctc tggctcatcc
     1981 acgagaacgt acgggtagac ggctggcgcg aggccgtgcc ggtcgtggtc gcgatcgccg
     2041 cgatcctcat cgttccgctc accggctggg gggtgcttct cacgggcctc gtgctgtctg
     2101 cattgctcgg gtataagttg gcaagccatg ggaaagccga catcggagaa ccagcggcgg
     2161 cttgatgccg ccgcgatgac ggcccgggtc cggcgcgcgg ccaacgtggt tggggtggcg
     2221 gtgattgcct ggatcgtcct gttcttcctg agctcggacg tcgtttcgct tcgcgctgac
     2281 agcccctggt ccgaggaccc ggcggacctc gtcgtgtcgc tgaccttcct gctgctcggg
     2341 gtggtcggcc cggtcacctt cgtccgggtt cagcgcgatg ccgaatcgtc cgtgatgcga
     2401 ccgcgaacag ccgacgacgt catgcgtggc ctgtacgtgg cactcgggac catcacggtg
     2461 gccgatgcca ccatgctctt ggcgtgggtg gactcggcac aggcaggcgg catggcctcg
     2521 attggattcg tgctggcaat gctgctgggc acttcgatcg ccaccgcggg agtggccgga
     2581 gtcgtggtgt atcgcgccgc gcgggtctcg agcagttggc gtcagtcgat cgaccgtgag
     2641 gaacgcgacg cgttggacga catcgtggca ttcaccctcc gcatccggcc actgcgcacc
     2701 tcggccgtcg ccttcgccgg gttcctgaat gggcggttca gcccgcgccg ccatcgctgg
     2761 gcgttcgcca ccctcgtggc cgtcggcttt gggctcggct acagcacctg gcacctgctg
     2821 gtggaaggcc cagcgccgag cgccagtgct tcgttcacgg tcctcatcgt gttcgcgtcg
     2881 ttcggcgcgg ccgcagtcct ggccggatgg atcgcctttg gcggctacct ccgactcgtt
     2941 cgtcgagtat ccgactgacg cggacctagt cgatcgcgaa ccagacccga acgcgtcggg
     3001 tcacggtcag gtcccctgcc gcgacctcgg tcggcgcgcc tgcgctgtcg gccatggcgg
     3061 ccatcgcccg catccggggc atcggcgcca tgccgccgtc ctcctcctcg acccggatga
     3121 cggcgcccag cgcaaccttc gcggcgcggg cgagcgcctg cgccttcgcg tgtgcggcgg
     3181 cgaacgcggc caccagtgcc tgatgctcgg ccgcggacgg gtctgacgcg ctcatctggg
     3241 caccctggac ctcgttcgcg ccggcggtca ctaccccgtc caggacctcg cccaggcggc
     3301 tcaggtcgcg gaccttgatc gtcatcgggc gcgcgacgcg atagccgatc agcttctgtc
     3361 cccggcggta gtcgtattcg gggctgatcg aaacgtccgg cgcgttgatg tcgccctcgg
     3421 cgatctccag ctcgcggagc gcgcccagca ccgcggacga gcgaaggacc acgtcgtccc
     3481 gggtcggggc gagctctttt ccctggccgc tgaccgccag cgagacaagg gcgaggtccg
     3541 gcgccacggt ggcttctccg tcggcggtca cgacgatcgc ccgacgcatc tggtccttca
     3601 tgtcgtctcc ttcattccgc ggtttcccag accgtcgcga tcccctggcc gacgccgatg
     3661 cacatggtgg ccagcccgta gcgtgacccg cgtcggcgca tctcgtgggc gagcgtcgcc
     3721 accaggcggg caccggatgc tccgagcgga tgaccgatgg cgatggcccc accgttgacg
     3781 ttgaccttct ccggatcaag ccccagctcg cgcatgacgg gtagtgcctg ggcggcgaag
     3841 gcctcgttca gctcgacgag gtccaggtcg ccgaccgaca ggccggcgcg ttcgagcgcg
     3901 aggcgggacg ccgggatcgg gccgagaccc atggttgccg gatccactcc ggccacggcc
     3961 gatgaacgca tccggagcat cggccgcagg ccggcatcgc gagcgcgctg ctcgctcatc
     4021 acgaccacgg ccgccgcccc gtcgttgatt ccgctgctgt ttcccgcggt gaccgatccg
     4081 gactcgtcct tgcggaacgc cggtttcagg ccggcgagct gctcgagggt ggtgtccggt
     4141 cgcggatgct cgtcccggtc gacggtcac
//
LOCUS       DASYGU010000291         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_461438, whole genome shotgun sequence.
ACCESSION   DASYGU010000291 DASYGU010000000
VERSION     DASYGU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461438"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VGO32_08400"
     CDS             complement(<1..539)
                     /locus_tag="VGO32_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HEV7200806.1"
                     /translation="MKIAVCVKQVPATTSEKRYTPDLRLDRAAAEAIVNPLDEYAIEQ
                     ALRLKEGGAADEIVYLTMGPESAAEALRRALAMGGDLGFLVTDPALAGADEWVTARVL
                     AAALDRIAPEVSLMGMSSDDARGSLVPGAVAAIRGLPLLAYGSELRLTDGAAQVRRLN
                     PTGFDILEAPLPVVASVTD"
     gene            complement(536..1732)
                     /locus_tag="VGO32_08405"
     CDS             complement(536..1732)
                     /locus_tag="VGO32_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7200807.1"
                     /translation="MATTPATQRPSGSAYRLTREHDDLRALVRQLADERIAPRAAEID
                     ATSEYPWDLKQLLAEHDLLGTGFEERHGGTALDGIGQAIVVEEIARADATTSLIPIIQ
                     KLGALPIMLAGSDEQKDRYFPRLASGEWLPAFALTEAGAGSDVASMRMRATREGDDYV
                     LNGTKRFITGGSVANLLTTFALTDPEAGGRKGMSGFIVKTDTPGFAVARLEHKMGIRG
                     SPTAELTFDDVRVPAGNRLGEEGDGFKIAMATLDRSRLAIAAQAVGIAQGATDSALAY
                     ASERKQFGDRIIDFQGIQFMLADMASLTEAARQLVYAASARVDDEDPNLAYWTSAAKL
                     VAGDTAMRVTTDAVQVYGGYGYSTDYPVERMMRDAKITQLYEGTQQIQRLVIARRLMA
                     QAGIGR"
     gene            complement(1780..2646)
                     /locus_tag="VGO32_08410"
     CDS             complement(1780..2646)
                     /locus_tag="VGO32_08410"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7200808.1"
                     /translation="MLERAGAPFRGFGDWLARVVDGFYRVLGQPGKWLQDFVNGSWLG
                     HPLHPVLTDVVIGGATLIAVFDLAVLVLGADGLETASLIGVGIVALSALGATTTGLTD
                     FKDTHTGNERNVVVLHGLINIVATAAYIVSFFLRLGGSESLGIWFSLAGILILTAGGY
                     IGGHIVFKYGYMVNRNAFAKGQRAKEFSPVIAAADVPESTPTKVMLGSTALVVVRRGD
                     VVHALKATCSHAGGPLDQGELVDDTIVCPWHFSAFRLADGAVRHGPATTRQVTYRARV
                     SDLQIEVEGPID"
     gene            complement(2734..>2961)
                     /locus_tag="VGO32_08415"
     CDS             complement(2734..>2961)
                     /locus_tag="VGO32_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV7200809.1"
                     /translation="AKGAVLFSPKRVTNYRDFIGIDEELWTAFYFFLANRGILLPPGS
                     DEQWTLSVAHSDADIERHIEVFGEFARELHG"
BASE COUNT          474 a          988 c         1041 g          458 t
ORIGIN      
        1 tggtcggtca cgctggccac gaccgggagc ggcgcctcga ggatgtcgaa gccggtgggg
       61 ttcagccgcc gaacctgggc cgccccatcc gtcaggcgca gctcggaacc gtaggcgagg
      121 agaggcagcc cccgaatggc agccaccgcg ccggggacga gcgagccgcg cgcgtcgtcg
      181 gacgacatcc ccatcaggct gacctcgggc gcgatgcggt cgagcgcggc ggccagcacc
      241 cgggcggtga cccactcgtc ggcgccggcc agcgccggat cggtcacaag gaatcccagg
      301 tcgccgccca tcgccagcgc acggcgcagg gcctcggctg cggactcggg tcccatggtc
      361 aggtacacga tttcatcggc ggcaccccct tccttgagac ggagggcctg ctcgatcgcg
      421 tactcgtcga gtgggttgac gatggcctcg gccgcggcgc ggtcgagccg gaggtcgggg
      481 gtgtagcgct tctcggatgt ggtcgccgga acctgcttga cgcagacggc gatcttcatc
      541 ggccgatgcc ggcctgcgcc atcagccgcc gggcgatgac gaggcgctgg atctgctgcg
      601 tgccctcgta cagctgggtg atcttcgcgt cgcgcatcat ccgttcgacc gggtaatcgg
      661 tcgaatagcc gtagccgccg tacacctgca cggcatcggt cgtgacgcgc atggcggtgt
      721 ccccggcgac gagcttggcg gcgctcgtcc aatacgcgag gttcgggtcc tcgtcgtcga
      781 cccgggcgga cgccgcgtac acgagctgac gcgcggcctc ggtcagcgaa gccatgtcgg
      841 ccagcatgaa ctggatgccc tggaagtcga tgatccggtc cccgaactgc ttgcgttccg
      901 aggcgtacgc cagcgccgaa tcggtcgcgc cctgggcgat tccgaccgcc tgggccgcga
      961 tcgcgaggcg agatcggtcc agggtcgcca tcgcgatctt gaagccgtcg ccctcctcgc
     1021 cgagccggtt gccggcgggc acgcgaacat cgtcaaaggt cagctcggca gtgggcgagc
     1081 cgcggatgcc catcttgtgc tcgaggcggg caaccgcgaa gccgggcgta tcggtcttga
     1141 cgatgaaccc gctcatcccc ttccggccgc cggcctccgg atcggtcagg gcgaaggtcg
     1201 tgagcaggtt cgccaccgag ccgccggtga tgaaccgctt cgtgccgttc aggacgtagt
     1261 catcgccctc gcgggtcgcg cgcatccgca tgctggccac gtcactgccg gcgccggcct
     1321 cggtcagggc gaacgcggga agccactcgc cggacgcgag ccgcgggaag tagcggtcct
     1381 tctgctcgtc gctgccggcc agcatgatcg ggagggctcc gagcttctgg atgatcggga
     1441 tcaggctggt ggtcgcatcc gcccgggcga tctcctcgac cacgatcgcc tggccgatcc
     1501 catccagcgc ggtgccgccg tgtcgctcct cgaagccggt gccgagcaga tcgtgctcgg
     1561 caagcagctg cttgagatcc cacgggtact cggacgtggc gtcgatctcg gccgccctgg
     1621 gcgcgattcg ttcgtctgcc agctgacgga cgagcgcacg caggtcatcg tgctcacggg
     1681 tgagccgata cgcggacccc gacggacgct gggtggcggg cgtagtggcc aaatcgccgc
     1741 gagtctaccc gaccacttcc ggtgtgcggg cgaatcagct cagtcgatcg gcccttcgac
     1801 ctcgatctgg aggtcgctga cgcgggcccg gtaggtgacc tggcgggtcg tggcgggccc
     1861 gtggcgcacc gcgccatcgg ccagccggaa ggccgagaag tgccacgggc aaacgatcgt
     1921 gtcatccacc agctcgccct ggtcgagcgg gccacccgcg tgcgagcacg tggccttcag
     1981 ggcgtggacg acgtcgccgc ggcgcacgac caccagcgcg gtgctgccga gcatgacctt
     2041 ggtcggggtg gactccggca cgtcggcggc ggcgatgact gggctgaact ccttcgcccg
     2101 ctggcccttc gcgaacgcat tgcggttcac catgtagccg tacttgaaga cgatgtgacc
     2161 gccgatgtag ccacccgcgg tcaggatcaa gatgccggcc agcgagaacc aaatgccgag
     2221 gctttccgag ccgccgagtc gcaggaagaa cgagacgatg taggcggccg tggccacgat
     2281 gttgatcagg ccgtggagga cgaccacgtt gcgttcgttg ccggtatggg tgtccttgaa
     2341 gtcggtgagc ccggtcgtgg tggctcccag ggcggacagg gccacgatgc cgaccccgat
     2401 gagactggcc gtttcgaggc catcggcccc gaggacgaga accgcgagat cgaataccgc
     2461 gatgagggtc gcgccaccga tgacgacatc ggtcaggacc ggatggagtg ggtggccaag
     2521 ccaggaaccg ttgacgaagt cctggagcca tttgccgggc tggccgagca cccggtagaa
     2581 accgtcgacg acacgggcca gccagtcccc gaagccgcgg aatggagccc cagctcgctc
     2641 cagcatggcc cgcagcctag caggccgcga gactacggtc tctatcggac aagttgactg
     2701 gcgttacacg cccaggacac cggcggctgc ggtctagccg tgcagctccc gcgcaaactc
     2761 accgaacacc tcgatgtggc gctcgatgtc ggcatcgctg tgagccaccg aaagggtcca
     2821 ctgctcgtcc gagccgggag gcaggaggat gccgcggttg gccaggaaga agtagaacgc
     2881 ggtccacagc tcctcatcga tcccgatgaa gtcgcggtag ttggtcaccc gcttggggct
     2941 gaacaggacc gcgcccttgg c
//
LOCUS       DASYGU010000292         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_305792, whole genome shotgun sequence.
ACCESSION   DASYGU010000292 DASYGU010000000
VERSION     DASYGU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305792"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1485)
                     /locus_tag="VGO32_08420"
     CDS             complement(190..1485)
                     /locus_tag="VGO32_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV7200810.1"
                     /translation="MATADGTSREAVDRLFREHHGRAVATLIGLLGDFDLAEESLQDA
                     YLVALEKWPAAGIPRRPEAWIVVTARNRAVDKLRRARRLTDKQAVIEREATLAAPAAE
                     DEALPEGADDEPMSIPDDRLRLIFTCCHPALALESRIALTLWTLGGLTTPEIARAFVV
                     PEATLAQRLVRAKRKIRDAAIPYRVPPDALLPERLDGVLRVLYLIFNEGYDASSGESL
                     IRHELCAEAIRLTRTLATLMPDEPEVAGLLALMLLTDARRAARVGPDGELILLEDQDR
                     GMWDRARIVEGQMLVERALRTGRAGPYQLQAAIAAVHGEAPSADVTEWAEIVRLYDAL
                     LQVAPNPIVELNRAAALAIATGPRAGPEAGLAIMDRLLAEGTLDDYPYLHAGRADLLR
                     RLGRPDEAATAYRRAAALTTNQAERAFIQRRLGELAATT"
     gene            complement(1505..1906)
                     /locus_tag="VGO32_08425"
     CDS             complement(1505..1906)
                     /locus_tag="VGO32_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV7200811.1"
                     /translation="MRYAILIYDANTADSSLPPPDPETWAKVTEAYGAYSKMLTDSGA
                     LVAGDALQPNATATTIRSKDGQTVMTDGPFAETKEGLGGFYIVEARDLDHALELGKAC
                     PGLWYGSIEVRPVWDAAVPADPQTAGEAVTA"
BASE COUNT          278 a          637 c          727 g          291 t
ORIGIN      
        1 gtgcgccgac gatggaatac agaaccagtc ataaccaagg accccaatac ggataagaac
       61 tcctcgaccg tacgccgcag gatggctggg ctcgatgagg tgtttcggcg cgcctgatcg
      121 gaccttcggg ccagggttcg aacggcgccg atggaggtga ccttggagcc aggtgccgcc
      181 agtcaccgct caggtcgtgg cggcgagctc cccgaggcga cgctggatga aggcccgttc
      241 ggcctggttg gtggtcaggg ccgcagcacg ccggtaggcc gtcgcagcct cgtccgggcg
      301 accgaggcgc cggaggagat cggcccgtcc ggcgtggagg taggggtagt cgtcgagcgt
      361 gccctccgcg aggaggcgat ccatgatcgc caggccggcc tcgggtccag cgcgggggcc
      421 ggtcgcaatg gccagcgcgg ccgcccggtt gagctccacg atggggttcg gcgcgacctg
      481 gagcagggcg tcatacaggc ggacgatctc ggcccactcg gtgacgtcgg cagatggcgc
      541 ctcgccgtgg accgcggcga tcgcggcctg gagctggtac ggcccggccc gacccgtccg
      601 cagcgctcgt tcgaccagca tctggccctc cacgatccgg gcccgatccc acatgccgcg
      661 gtcctggtcc tcgagcagga tcagctcgcc gtcggggccg acccgggctg cacgccgggc
      721 gtcggtcagg agcatgagcg cgagcaggcc ggccacctcg ggctcgtcgg gcatgagcgt
      781 tgccagggtc cgggtcaggc gaatggcctc tgcgcacagc tcgtggcgga tcaggctctc
      841 gcccgatgac gcgtcgtagc cctcgttgaa gatcaggtac aggacgcgga gaaccccgtc
      901 gagccgctca gggagcagag cgtcgggcgg gacgcggtat gggatggcgg catcgcggat
      961 cttccgcttg gcccggacga ggcgctgggc gagggtggcc tcgggcacga cgaacgcgcg
     1021 cgcgatctcc ggcgtcgtga gcccacccag cgtccacagc gtcaacgcga tccgcgactc
     1081 gagggcgagg gccggatggc agcaggtgaa tatcagccgg agccggtcat cggggatgga
     1141 catcggttcg tcatccgctc cctcgggaag ggcctcgtcc tccgccgccg gggcggcgag
     1201 ggttgcctct cgttcgatta ccgcctgctt atcggtcagt cgtcgggcgc gccggagctt
     1261 gtccacggcc cgattccggg cggtgaccac gatccaggcc tcggggcggc ggggaatgcc
     1321 cgcggccggc cacttctcga gcgccacgag gtaggcgtcc tggaggctct cctcggccag
     1381 gtcgaagtcg ccgagcagcc cgatgagagt ggcgaccgcc cgtccgtggt gttcgcggaa
     1441 caggcggtcg acagcctcgc gggacgtccc gtcggcggtc gccaacggcg tcagagggcc
     1501 acggtcaggc ggtgacggcc tcacccgcgg tctgcgggtc agccggcacg gccgcgtccc
     1561 acaccgggcg gacttcgatc gacccgtacc acaggcccgg gcaggccttg cccagctcaa
     1621 gggcgtggtc caggtcgcga gcctcgacga tgtagaagcc gccgaggcct tccttcgtct
     1681 cggcgaaggg gccatcggtc atgaccgtct ggccgtcctt ggagcggatg gtcgtggcgg
     1741 tcgcgttggg ctggagcgcg tcgccggcga cgagggcgcc ggagtcggtc aacatcttgc
     1801 tgtacgcgcc gtaggcctcg gtcaccttgg cccacgtctc ggggtcaggc ggcggaagcg
     1861 aggagtcggc ggtgttcgcg tcgtagatca ggatggcgta gcgcatcggg tcctccagtg
     1921 atttggctca cgc
//
LOCUS       DASYGU010000293         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_615438, whole genome shotgun sequence.
ACCESSION   DASYGU010000293 DASYGU010000000
VERSION     DASYGU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615438"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGO32_08430"
     CDS             complement(<1..425)
                     /locus_tag="VGO32_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7200812.1"
                     /translation="MSAASPARALEPGVSRGAGARLVIDDVHKRYGPVGNHVLALGGI
                     SLTVDPGEFVCLVGASGCGKSTLLNLVAGLDQPSRGTVDVGTAHTTLMFQEAALFPWL
                     TAGQNISLALKYSGVPKGKRKNRIEELLDLVRLSGHSDRR"
     gene            complement(422..1465)
                     /locus_tag="VGO32_08435"
     CDS             complement(422..1465)
                     /locus_tag="VGO32_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7200813.1"
                     /translation="MGRTMVGRGLAMAVILVIGLVACQPTETPSGPVNLRLGYFPNLT
                     HATAIMGIEDGILAEALGSDVELAPQLFTAGPEVVEAIFSGALDASYIGPNPAINAFI
                     RSASDGGAIRIVSGATSGGASLVVRDGIDGPEDLAGARLGSPQLGNTQDVALRAWLAD
                     QGYETDLEGGGEVEILPQGNSQSLEAFVAGDLDGAWLPEPWVTRMVDEAGGHVLVDER
                     DLWPDGQFVTTHLIVATAFLEAHPDVVKRLLEGHVAATEAVNADPAHAQEVVAAAIAD
                     LSGTEVAVGLLASAWDKMTFTVDPIASSLTTSAENAVELGLLDSADLEGIYDLSLLNE
                     VLAEAGASPIPEP"
     gene            1514..>1694
                     /locus_tag="VGO32_08440"
     CDS             1514..>1694
                     /locus_tag="VGO32_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200814.1"
                     /translation="MASAAGPSSTGVGKEAVRPGTRQRHADRRLTHTALEARLRSLEE
                     GEVLGLDDLVGGRCAD"
BASE COUNT          277 a          580 c          572 g          265 t
ORIGIN      
        1 cgccggtcgg agtgaccgga caggcgcacc aggtcgagca gctcctcgat gcggttcttt
       61 cgcttgccct tcggcacccc cgagtacttc agggccagcg agatgttctg tcccgcggtc
      121 aaccacggga acagggccgc ctcctggaac atcaacgtgg tgtgtgcggt accaacgtcg
      181 acggtgccgc ggctcggctg gtcgagcccg gcgacgaggt tcaggagggt gctcttgcca
      241 cagccggacg cgccgacgag gcatacgaac tcgccgggat caacggtgag cgagatgcca
      301 cccagggcga ggacgtggtt gccgaccggc ccataacgct tatggacgtc gtcgatgacc
      361 aatcgcgccc cggctcctct ggacacgccg ggttcaagcg cccgcgcggg gcttgccgca
      421 ctcatggctc cgggattggg ctcgcccccg cctcggccag gacctcgttc aggagggaca
      481 ggtcatagat gccctccagg tcggccgagt cgaggaggcc gagctcgacg gcgttctccg
      541 ccgacgtcgt cagggagctc gcgatgggat cgaccgtgaa cgtcatcttg tcccacgcgc
      601 tggccaacag cccgaccgca acctccgtcc ccgacagatc ggcgatggct gcggccacca
      661 cctcctgggc gtgcgccgga tcggcgttga ccgcttcggt ggcggcgacg tgaccctcga
      721 gcagccgctt cacgacgtcg gggtgcgcct ccaggaatgc cgttgccacg atcaggtgcg
      781 tcgtcacgaa ctggccgtcg ggccacaggt cacgctcatc gacgaggaca tggccgccgg
      841 cttcgtcgac catccgggtg acccagggct cggggagcca cgctccgtcg aggtcgccgg
      901 cgacgaacgc ctccaatgac tggctgttgc cctgcggcag gatctcgacc tcgccgcctc
      961 cttcgagatc ggtctcatag ccctggtcag ccagccaggc gcgcagggcc acgtcctggg
     1021 tgttgcccag ctgcggcgag cccagtcgag cgccggccag gtcctcgggt ccgtcgatcc
     1081 cgtcacgaac gacgagcgac gcgccaccgg acgtggcacc ggacacgatc cggatggcgc
     1141 cgccatcact ggccgagcgg atgaaggcgt tgatggccgg gttcggcccg atgtaggacg
     1201 cgtccagggc cccgctgaag atggcctcca cgacctccgg accggccgtg aacaactgcg
     1261 gcgcgagctc gacgtcgctg ccgagcgcct cggcgaggat cccgtcctcg atccccatga
     1321 tggccgtggc atgggtcagg ttcgggaagt atcccagccg gaggttcacg ggaccactcg
     1381 gcgtctcagt cggctggcat gccaccaggc cgatgaccag gatgaccgcc attgccagtc
     1441 cgcgcccaac catcgttcga cccatgttta ccttctccat gaagtagatc aagttaatcg
     1501 gagaagttgc attatggcat cggccgccgg tccgtcgtca acgggggtcg gcaaagaagc
     1561 ggtgcgcccg ggcacccggc agcggcacgc ggacaggagg ctcacccaca ccgcgctcga
     1621 ggcgcggctc aggtcattgg aagaagggga agtcctcggc ctcgatgacc tggtcggcgg
     1681 gcggtgcgct gatg
//
LOCUS       DASYGU010000294         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1489203, whole genome shotgun sequence.
ACCESSION   DASYGU010000294 DASYGU010000000
VERSION     DASYGU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489203"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VGO32_08445"
     CDS             complement(<1..420)
                     /locus_tag="VGO32_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7200815.1"
                     /translation="MGPVIGLLAAIALGYLLGAIPFGVVIARLAGGDDPRTVGSQRTG
                     ATNAVRALGPRWGLVVGLLDVAKGLVAAAAGAAIGAAVGLVPEWVAAGSCVAAVTGHI
                     RSVFIGFRGGRGVATAAGGFLVLVPLALVIVIPIIGAV"
     gene            complement(435..749)
                     /locus_tag="VGO32_08450"
     CDS             complement(435..749)
                     /locus_tag="VGO32_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200816.1"
                     /translation="MSRAERRQYQRMTKNQDPYASPSGGKGRRPPRKRGPVAPRDWSF
                     TRGFWLKIIGIAAVVGLIGLSVTWPGGAERALMVGAATAVAAFVLLVAARLFLQRRST
                     AG"
     gene            788..>2916
                     /gene="aceE"
                     /locus_tag="VGO32_08455"
     CDS             788..>2916
                     /gene="aceE"
                     /locus_tag="VGO32_08455"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEV7200817.1"
                     /translation="MHDVFDQFKQQLPDADPTETDEWLDSLDDVVERAGPERARFILY
                     RLLKRARQLQVGLPALTQTRYVNTISPEQEPTFPGDEQMELRIRRMIRWNALAMVLRA
                     NTRFEGIGGHLSTYASAASLYEVGFNHFFRGWDDGPGDLVYFQGHAAPGVYARAFLEG
                     RLTTDQLDHFRREALTPTGLPSYPHPRTMPDFWQFPTVSMGLGPLAAIYQARFNRYLA
                     ARGLADTASSRVWAFVGDGEMDEPEALAGLSLAAREGLDNLIFVVNCNLQRLDGPVRG
                     NGKIIQELESVFRGAGWHVIKVIWAREWDALLERDTDGVLLNKMTETLDGDYQTLSVS
                     DGAYVREHFFGPDPRLQAMVADKTDAELAALRRGGHDYRKLYAAYRYATEHTGAPVVI
                     LAKTVKGWTLGPGIEGRNVTHQAKKLTTDELKIFRDRLQLPIPDDQLPDAPYYHPGAD
                     SPEIQYLLERRKSLGGVLPRRRTETKALPAPDESVYADVLGGAAQPASTTMAFGRLLR
                     NLVRDPEIGRRVVPIIPDEARTFGMDPLFKELGIYNPLGQRYAAVDRQLLLSYREATD
                     GQILEEGITEAGSMASFTAAGTAYSVHDEPLIPFYVFYSMFGLQRTGDQAWAFGDARG
                     RGFMMGATAGRTTLTGEGLQHDDGQSHLYALAIPNLQAYDPAFAYELGVIVRDGIRRM
                     WQNGEDVFYYITLYNENWVQPAMPEGVT"
BASE COUNT          464 a         1074 c          981 g          397 t
ORIGIN      
        1 caccgccccg atgatcggga tcacgatcac gagcgcgagg ggcacgagga cgaggaagcc
       61 gccggccgcg gtggccaccc cccggccgcc gcggaagccg atgaacaccg agcggatgtg
      121 gccggtcacg gcggccacgc agctgcccgc cgcgacccat tccggcacca gcccgaccgc
      181 cgcgccgatg gccgccccgg cggccgccgc caccagcccc ttggccacgt ccagcagccc
      241 cacgacgaga ccccagcgcg gaccgagcgc ccgaaccgcg ttcgtggccc cggtccgctg
      301 cgatcccacc gtccgcgggt cgtcgccgcc ggcgaggcgg gcgatgacca cgccgaacgg
      361 gatcgcgccc aggaggtagc ccagggcgat ggccgccagc agaccgatga cgggtcccac
      421 ggcctcagtc ggcctcagcc ggccgtcgac cgccgctgga ggaacaggcg cgcggcgacg
      481 agcaggacaa aggcggccac ggcggtcgcg gcccccacca tcagcgcccg ctcggcgcca
      541 ccgggccacg tcacggacaa cccgatgagt cccaccaccg cggcgatgcc gatgattttc
      601 agccagaaac cgcgggtgaa cgaccagtcg cgcggcgcga ccggtccgcg ctttcggggc
      661 gggcgccgac ccttgccgcc ggacggcgag gcatacggat cctggttctt cgtcatccgc
      721 tggtactggc ggcgttcggc gcgcgacatc ggtccctcag tctatctgcc ggtaggattg
      781 ggccgccgtg cacgacgtct tcgaccagtt caagcagcag ctgcctgacg cggacccgac
      841 cgaaaccgac gaatggctcg attcgctgga cgacgtcgtc gagcgggccg gtcccgagcg
      901 ggcgcggttc atcctgtatc ggctcctgaa acgggcccgc cagctgcagg ttggcctgcc
      961 ggccctgacc cagacccggt acgtgaacac gatctcgccc gagcaggaac cgacgttccc
     1021 cggcgacgag cagatggagc tccggatccg gcgcatgatt cgctggaacg cactggccat
     1081 ggtcctccgt gcgaacaccc ggttcgaggg catcggcggc cacctgtcga cctacgcctc
     1141 ggcggcgtcg ctgtacgagg tgggcttcaa ccacttcttc cgcggttggg acgacgggcc
     1201 gggcgacctc gtctatttcc agggccacgc cgcgccgggg gtctacgccc gggccttcct
     1261 cgaggggcgg ctgaccactg accagctcga tcacttccgc cgtgaggcgc tgaccccgac
     1321 cgggctgccc tcctaccccc acccgcggac gatgcctgac ttctggcagt tcccgaccgt
     1381 gtccatgggc cttgggccgc tggccgccat ctaccaggcc cgcttcaacc gctatctcgc
     1441 ggcccgcggc ctggccgaca ccgcgagctc gcgggtgtgg gcgttcgtgg gcgacggcga
     1501 gatggacgag cccgaggcgc ttgccgggct gagcctggcc gcgcgcgagg gcctggacaa
     1561 cctcatcttc gtcgtgaact gcaacctcca gcgcctggac ggcccggtcc ggggcaacgg
     1621 caagatcatc caggagctgg aatcggtgtt ccgcggcgcc ggctggcacg tgatcaaggt
     1681 catctgggcc cgcgagtggg acgccctgtt ggaacgcgac accgatggcg tgctcttgaa
     1741 caagatgacc gagaccctcg acggcgacta ccagaccctg tcggtgtcgg acggggcgta
     1801 tgtccgcgag cacttcttcg gccccgaccc acgcctgcag gccatggtcg cggacaagac
     1861 cgatgccgag ctggccgccc tccggcgtgg cgggcacgac taccgcaagc tgtacgccgc
     1921 ctatcggtat gcgactgagc acacgggggc cccggtcgtg atcctggcca agacggtgaa
     1981 gggctggacc ctgggcccgg ggatcgaggg tcggaacgtc acccaccagg ccaagaagct
     2041 gaccaccgat gagctgaaga tctttcgcga ccggctccag ctgcccatcc cggacgacca
     2101 gctgccggat gcgccgtact accaccccgg cgccgacagc cccgagatcc agtacctcct
     2161 cgaacgacgc aagtccctgg gcggggtcct cccccggcgg cggacggaga ccaaggcgct
     2221 ccccgcgccg gacgaatcgg tctacgccga tgtcctgggc ggagcggccc agccggccag
     2281 cacgacgatg gccttcggcc gcctgctccg gaacctcgtt cgggacccgg agatcggccg
     2341 ccgggtggtg cccatcattc ccgacgaagc gcgtacgttc gggatggacc ccctgttcaa
     2401 ggagctgggc atctacaacc cgctcggcca gcgctacgcc gcggtcgacc gccagctgct
     2461 cctctcgtac cgggaggcaa ccgacggcca gatcctggag gaaggcatca ccgaggcggg
     2521 atcgatggcc tcgttcaccg cggccgggac cgcgtactcg gtccatgacg agccgctcat
     2581 cccgttctac gtcttctact cgatgttcgg cctgcagcgg accggcgacc aggcctgggc
     2641 gttcggggac gctcgggggc gcggcttcat gatgggggcg accgcgggcc gcaccacgct
     2701 caccggggag gggctccagc acgacgacgg gcagagccac ctgtacgcgc tggccatccc
     2761 gaacctgcag gcctacgacc ccgccttcgc ctacgagctg ggggtcatcg tccgcgacgg
     2821 gatccgccgg atgtggcaga acggcgagga cgtcttctac tacatcaccc tgtacaacga
     2881 gaactgggtc cagccggcca tgcccgaggg ggtcac
//
LOCUS       DASYGU010000295        13846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_49978, whole genome shotgun sequence.
ACCESSION   DASYGU010000295 DASYGU010000000
VERSION     DASYGU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49978"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..975
                     /locus_tag="VGO32_08460"
     CDS             286..975
                     /locus_tag="VGO32_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200818.1"
                     /translation="MTRRFKILGIGMVLIGFAFVAAGGYSYIKVQEGYRSLNAFSAAQ
                     NVALSYNEDGQLVDGGDPAEAAVIMALLTDEWGYPVVASDLDPNDPVVNTASEYMFEM
                     ATIATHIMHGTQTIVLDEPVEYNGETFAAGEYEFAIDGRYYADFDRAHPLEGPARGLA
                     WSPTALGLIGQLGVGAVTHSTLQMGLGLAAALGAVGGALLVLGGGLVWAVRPEREAVP
                     AIQPVTTPIPA"
     gene            1066..4302
                     /locus_tag="VGO32_08465"
     CDS             1066..4302
                     /locus_tag="VGO32_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7200819.1"
                     /translation="MTEERRLVTILFADVAGSTALGESLDPEDMRALLGRYYEIAREV
                     VAAHGGTLEKFIGDAVMAVFGLPTAHDDDAARALAAALALRDQVRGDARLGERLPIRL
                     GLSTGEVVAAMGGTGDDFLVTGDAVNVAARLQQGAESWEIVCAERTVRAAGDGFAFGE
                     PIQVDARGKRAAVPARRLHEARAARPRPRTPFLGRDADVAQLELTSGRSMKERRPYLI
                     TITAPAGTGKSRLLEEFLARLPEEQPGARVVTAQCLPYGQRLTYWPLHALLLQLLHLP
                     EDGDLDAIRPAAAAWLEAAGDPLPRRTAELLVATFGAGEGDPIDRAELFAAWRTAIEL
                     AAREHPLVVVVEDLHWSSDSLLDLIEVILQPRGEASLLMIVLARPELLDRRPTWGGGR
                     RNYVALNLEPLDELAIAALADYLLQGPPQEVVDTVVARAEGNPFYAGELARALRERAP
                     DLDDPAAVTAAVAALPDTVHATVLARLDLLAPAPRRAIQLGSVFGRSFQTGGVAALEP
                     ELAGNLTDTINHLLEHDLLRQGIGDELTFRHILIREVAYQTLPRAERARLHGAAGAWL
                     ETQSAGHEEEVAELVAFHFREAVTLGTAAGIDLDVGTIDKAVTWLNRAATAATAGAAY
                     EEAGRHLRAAIEIAPREELPELWARLGDIFGGGNQATEAYANAARIGKELGRSPDFVL
                     RAMSGELMVLGRWGGSVGSPLAPGEVEALVADVHALRSEATDRAVIGWSLVAESFIPF
                     EAATSDRIATKDEWDNARTIAEQGLAIARELDDAPMISAALDALSSYTLGTDPEAGVR
                     FALERKALEDRLPLYERTDTQNMLAWSSAALGKLTTVLDAAESILRDLAPNQAQALAL
                     SLAGWQCWALGLLGRWDEMSPATERASRLWEEAGRISAGFAMHAFIVAADVGRARQDD
                     RLRSRAVATLLGIGEQFPPGQLFRRLTAFAEPDPEALVRNVVLDWQPYITRVHYVERA
                     VALCADVRHPIPIDGLDTLLAESRTRDQRLLTAQLLRARGNQERDEGNVREALELFRG
                     FGAGPLVARCEIELGQLVDDPDLVTSGTAVLDALGDMTHLSRVAALRR"
     gene            complement(4315..7497)
                     /locus_tag="VGO32_08470"
     CDS             complement(4315..7497)
                     /locus_tag="VGO32_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEV7200820.1"
                     /translation="MVSRRLIHLFLALVLGVGLWVPQLFTPPVSRAATMAGFSESIAF
                     SGLTNPMVIRFSSDGRVFVGEKSGLIKVFDSLADPTPTVFADLRTNVFNFWDRGLMGM
                     ALAPTFPSDPSVYVLYAYDALPGGTAPHWGSPGVTSDGCPSPPGPTTDGCVVTGRVSR
                     LTANGNQAGPEAVLITDWCQQYPSHSVGSLEFGADGYLYVSGGDGASWTFADYGQGGG
                     SGNSPTPENPCGDPPVPVGGNQTAPTAEGGALRSQDLRTGGDPVALSGAVLRLDPATG
                     LGAPQNPLAGSSDVNARRIIAEGFRNPFRFTVRPGTNELWIGDVGWDAFEEINRFVPS
                     GTVANFGWPCYEGSGRQAGYDAANLNICENLYAAGAGAVVAPYFAYNHAAKVVNGENC
                     AVGSSATAGMAFSAPGSYPSQYTGALFFADNPRDCIWVMYAGANGLPDPTTRATFVSG
                     AANPVDLEVGPGGDLFYVDFDGGTIRRISHAGNGAPTAFVSAVPSSGPAPLSVNFSAA
                     GSSDPNGDPLTYAWDLDGDGQFDDGTGSTASRTYALGTVTVALRVSDGHGGTGTATTP
                     VTATSGGPTVTYLSALTPTVATNGWGPYERDRSNGEALAGDGGPITLAGAVYARGLGT
                     HAASDLRYTVPAANCTFLASIGLDDEVGSQGSLTFQVQADSTTLYTSPTMTGASATRA
                     LSLVLPAGASLLRLNVPPGPSKNHDHGDWADARFSCGAGGSSNTPPQPVIDTPTVGTT
                     WAVGQSVAFTGHATDAQDGTLAAARLSWKLIVQHCPSNCHSHDIQTWSGTAGSSFAAP
                     DHDYPSYLDLQLTATDSGGLSTTVTRRLDPRTVNLSFASSPSGLQLTVGASSQATPFT
                     RTVIVGSATSVSASSPQSMGGTNYQFSTWSDGGAASHTIVAPATSATYTATFTVASGG
                     PTVTYLSALTPTTATNGWGPYERDRSNGEDLAGDGGPITLAGVVYARGLGTHAASDLR
                     YTVPAGSCSFLASVGLDDEVGSLGSLTFQVQANGATIYTSPTMTGTSATRSLNLALPA
                     GTSLLSLIVPTGPSKNYDHADWADARFSCL"
     gene            7712..8941
                     /gene="lhgO"
                     /locus_tag="VGO32_08475"
     CDS             7712..8941
                     /gene="lhgO"
                     /locus_tag="VGO32_08475"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEV7200821.1"
                     /translation="MAEAGRYDVVVIGGGLLGLATARNLLTDGPPGLRIAVLEQESDI
                     ALHQSGRNSGVIHRGIYYAPGSLKARLCTTGSRRLTAYCDEKGIPWERTGKVIVALNE
                     QELPRLDELYRRGVANGVPGLKVIGPEHLRELEPSAVGVRAVHSPETGIVDFRRVAQA
                     YADDVVHAGGEIRLESRVRSIEERGDEVRVMTDGGEIGTRYLIACAGLQSDRVAAMTD
                     ASVRRAMRILPFRGDYFVLRPERSFLCRSLIYPVPDLRFPFLGVHFTKRMDGSIWAGP
                     NAVLALARERYGRADVRIADVVDTVTFAGFWHLAMAHWRMGMSEAWRDVARHAFGDAL
                     RRFVPDLRDDDLLPGPAGNRAQAVAADGSLVDDFRITASGRSIHVRNAPSPGATSSLA
                     IGEMIARHARRRFTLDA"
     gene            complement(9025..10788)
                     /locus_tag="VGO32_08480"
     CDS             complement(9025..10788)
                     /locus_tag="VGO32_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEV7200822.1"
                     /translation="MAPRTFSRISLSLCALVIASTLPALSVAAAPGDVSRQSGINRYA
                     TAAAISHDTFSPGVNVAYVVNGLGFADALGAGAAAAELGGPLLLVTQSSIPSETATEL
                     DRLNPDEIIVAGGIGVVSDAVKTALGAYSSTVNRQAGLNRYATAAAISAGTFDPGVNV
                     VYVANGLGFPDALAAGPAAAHLGGPLLLVSQCAIPSETATELDRLNPGQIIIAGGTGV
                     VCAALESQLQAFIPGMPPNANNDNLGNVKSDCKWQYRVLNNDTDAEGATLSITAVSDP
                     AHGTAGILTHRLGGVEIPRVVSYVSDSGYTGADSFNYTISDGAGGSDTATVNLTVIAN
                     SGDTDGDGQPDECDAFATDGTNASGRTLPFSLTFDAGGGEIEDGGFTGLMTNSFTPSL
                     TQYNPALVGLDGSGHFVVDQVPEGDSANGGNDLKNGFQVNVTMPVSSFTVHGRVCSPY
                     PPGVFASAGIIIGPGNQDNYIKAVIETSTGTGVHDSREVNGEGSIIAAKLDAAIGSAA
                     CTDLYLYIDTVAHTYSPTYSTNNGVTRSGFTTGAGARTVPASWTDGTQALAVGIFSTS
                     QGPASPYTATFDLLEVHAGLP"
     gene            complement(11021..12160)
                     /locus_tag="VGO32_08485"
     CDS             complement(11021..12160)
                     /locus_tag="VGO32_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7200823.1"
                     /translation="MSTQSHEPEDLPTAQVGSTSLRISRLGLGTVPLGNMMGLIEDDE
                     ARSIVETAHRLGVRLIDTAPQYGAGLAEKRVGEAISMIPRDELVIATKVGRLLRRVST
                     ARKVASVLNQSVRGPERGPALIGRHAARFGRRILGRDPTYPLGFPFDRGDERALDSYF
                     DFSYDGVMRSVEESLTRLGLDRLDMLYIHDPDNHYDKALSGAFRALDRLRGDGSVQAI
                     GVGMNQSQMLTRFAEDADFDCFLLAGRYTLLNQSGLRDLLPTALRRGMTVNLGGVFNS
                     GILANPKPGASFDYHAVGSDAEPLQRALKMQAVCARYDVPLAAAALQFPLAHPAIGSV
                     LVGVRSADEIKENVAHFTRTIPDDLWRELRSEGLVPEDVPLPGGA"
     gene            complement(12157..12600)
                     /locus_tag="VGO32_08490"
     CDS             complement(12157..12600)
                     /locus_tag="VGO32_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200824.1"
                     /translation="MTSPARLQILLVALLGLAASFAIAADELPLREITVAVFLAIGPG
                     LAVLWLTGITDRVARVALVVPVSLSIDALVVAITVYLGVWSPELVMLALVGLTVGAIA
                     LIPYERPARAALILVALLPVLAIVAGEISVDLLTTGLTDISRYRL"
     gene            complement(12597..>13846)
                     /locus_tag="VGO32_08495"
     CDS             complement(12597..>13846)
                     /locus_tag="VGO32_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200825.1"
                     /translation="LVNWVGQDYFSPQAFNLLLLLAVLAVLLTWFRRSGSAGSTGGGT
                     SSARLRLRSLLAAILVDPAGSIEAVRAGRSISVRQQIGLIAIVAAMFGTSVASHQLTP
                     FALVGGVIGLTIFNRIRLTGLAMFMVVLLSLWLMFMATTFLSGHLASLLEQIFRPENI
                     DSTLARLVGSRGHLFVLQARLVLTLGVWVLALIGGLRRLRSGQLDLTLAVLAIAPFGL
                     ILLQAYGGEMLLRVYLFSGPFMAFFAAAAFFPTMRPGTWRTFGALGLLSITLASVMLL
                     TRYGNEKADYVTAAEYRVMQYVASVAQPGDTIGSANHSVPLGYIEWEQHRHVALDSEW
                     RFGDMDLVVLELTRRTPEANDSYLIITRGQRAYAELFWNMSDARWDHRVQAMAQRAEL
                     LFANADGAVYRVPPPAEPAEPDR"
BASE COUNT         2215 a         4591 c         4899 g         2141 t
ORIGIN      
        1 gcaccgctcc tcgagcgacg agccgccgcg cgcgctcagc gggacttcgc ggccgccgac
       61 gcgctccgcg aggaactcgc tgcgatgggg gtggaggtcc gggacacgcc agacggccag
      121 gtcgcgaccc gccgccgata gggatcgggc caaaggggac cgccaagcgg ggggccacat
      181 ggcccccgtt ctcggtccgt tcgccactga gcggaatgcg accgatgcgc cagcgttcca
      241 cttgagttca taagaaatgc gaaccgtgga atgaggtaca gggaaatgac tcgtcgattc
      301 aagatcctcg gcatcgggat ggtgctgatc ggcttcgcct tcgtggcggc cggtggctac
      361 tcatacatca aggtccagga aggctaccgg tcgctgaatg cgttcagcgc cgcacagaac
      421 gtcgccctca gctacaacga ggacggccag ctcgtcgacg gcggagatcc agccgaggcg
      481 gcggtcatca tggcgctgct caccgacgag tggggctacc cggtcgtggc gtccgatctc
      541 gatccgaacg acccggtggt gaacacggcg agcgagtaca tgttcgagat ggcgaccatc
      601 gccacgcaca tcatgcatgg gacccagacg atcgtgctcg atgagccggt cgagtacaac
      661 ggtgagacgt tcgccgccgg cgagtacgag ttcgccatcg acgggcgcta ctacgccgac
      721 ttcgaccggg cccacccgct cgagggcccg gctcgcggcc tggcctggag cccgaccgct
      781 ctcggcctga tcggccagct cggcgtgggc gccgtcaccc attccaccct ccagatgggc
      841 ctcgggctcg ccgccgcgct gggcgcggtc ggcggagcgc tcctggtcct cggtggcgga
      901 ctggtgtggg cggttcggcc cgagcgcgag gcggtgccgg cgattcagcc ggtcacgact
      961 ccgatcccgg cctgaagccg catggtgcgg gtccgaccgc accgactccg aaaccgggcc
     1021 gccgtcgcgg cccggtttcg cggtctgccc gggtagcatg gggtcgtgac tgaagaacgc
     1081 cggctcgtca cgatcctgtt cgcggacgtc gccggctcga ctgccctggg cgagtcgctc
     1141 gatccggagg acatgcgggc cctcctgggc cgctactacg agatcgcgcg ggaggtcgtc
     1201 gccgcccacg gcggcacgct cgaaaagttc atcggtgatg cggtgatggc cgtgttcggc
     1261 ctccccaccg cgcatgacga cgatgccgcg cgagccctgg ccgccgccct cgccctccgc
     1321 gaccaggttc gaggcgacgc gcgccttggg gagcggctcc ccattcgact cggcctcagc
     1381 accggcgagg tcgtcgcggc gatgggcggg accggggacg acttcctggt gaccggcgac
     1441 gcggtcaatg tcgctgcccg cctccagcag ggggccgaga gctgggagat cgtgtgcgct
     1501 gagcgcacgg tgcgcgccgc cggggatggc ttcgccttcg gggagcccat ccaggtcgac
     1561 gcccgcggca agcgggcggc ggtgcctgcg cggcgactcc atgaggcgcg tgccgcccgg
     1621 ccgcggccca ggaccccgtt cctgggccgt gacgccgacg tggcccagct cgagctgacc
     1681 tcgggccggt ccatgaaaga acgccgcccg tacctgatca cgatcacggc cccggcgggc
     1741 acggggaagt cccgtctgct cgaggagttc ctggctcgcc tgcccgaaga gcagcccggc
     1801 gcccgggtcg tgaccgcgca gtgtcttccc tatggccagc gactgacgta ctggccgctc
     1861 catgccctcc tcctccaact cctccacctg cccgaggacg gcgacctgga cgccattcga
     1921 ccggccgcag cggcgtggct cgaggccgcc ggcgatccac tcccgcgccg gaccgccgag
     1981 ttgctggtcg cgacattcgg cgccggcgaa ggcgacccca tcgaccgggc ggagctgttc
     2041 gcggcgtggc gcacggcgat cgagctcgcg gctcgcgagc atccgctggt cgtcgtggtc
     2101 gaggacctcc actggtccag cgacagcctg ctggacctaa tcgaggtcat ccttcagccc
     2161 cggggcgagg cgtcgctgct catgatcgtg ctcgctcgtc ccgagctcct cgatcgacgc
     2221 ccgacgtggg gtggtggacg acgaaactac gtagccctca acctcgagcc gctggacgag
     2281 ctggccattg ccgcgctggc cgactacctg ctccagggac cgccgcagga ggtggtcgac
     2341 accgtcgtcg cccgggccga ggggaatccg ttctatgccg gcgagctggc ccgggccctc
     2401 cgtgagcgcg cgcccgatct cgacgatccg gcggcggtga ccgcggcggt cgctgccctg
     2461 ccggacacgg tccacgccac ggtcctcgcc cgcctcgacc tcctcgcgcc ggcgccgcga
     2521 cgcgcgatcc agctcgggtc cgtcttcgga cggagcttcc agaccggcgg ggtggcggcc
     2581 ctcgagcccg agctggcggg aaacctcacc gacaccatca accaccttct cgagcacgac
     2641 ctgctccgcc agggcatcgg ggacgagctc accttccgcc acatcctcat tcgcgaggtc
     2701 gcgtaccaga ccctgccccg ggccgaacgc gcgcgccttc acggggcggc gggggcatgg
     2761 ctcgagaccc agtccgcggg acacgaggaa gaggtggccg agctggtcgc cttccacttc
     2821 cgggaggccg tcacgctcgg gacggccgcg gggatcgatc tcgacgtggg cacgatcgac
     2881 aaggcggtga cctggctcaa tcgcgcggcc accgccgcga cggccggtgc cgcgtacgaa
     2941 gaagccgggc gtcacctgcg cgcggcgatc gagatcgccc cgcgagaaga gctccccgag
     3001 ctgtgggctc ggctgggcga catcttcggt ggtgggaacc aggccaccga ggcgtatgcg
     3061 aacgcggcac ggatcggcaa ggagctgggc aggtcacccg atttcgtgct gcgcgcgatg
     3121 agcggcgagc tcatggtcct cgggcgttgg ggtggctcgg tcggctcccc actcgcgccg
     3181 ggcgaggtcg aggccctcgt cgcggacgtg catgcgctgc gctcggaggc gaccgatcgc
     3241 gccgtgatcg gctggtcgct ggtcgccgag tcgttcatcc cgttcgaggc ggccaccagc
     3301 gatcggattg ccacgaagga cgagtgggac aacgctcgga caatcgccga acagggtttg
     3361 gccattgccc gcgagctgga cgatgcgccg atgatcagcg ccgccctcga tgcactgtcg
     3421 tcgtacacgc tgggtaccga ccccgaggcg ggcgtcaggt tcgcgctgga gcgcaaggcc
     3481 ctcgaggatc ggctcccgct ctacgagcgg acggacacgc agaacatgct cgcctggtcc
     3541 tcggccgcgc tcggcaagct caccactgtc ctcgacgcgg cggagagcat cctccgcgat
     3601 ctcgccccca accaggccca ggcgctggcg ctcagcctcg ccgggtggca atgctgggca
     3661 cttggcctgc tgggtcgatg ggacgagatg tcgccagcca cggaacgagc gtcacggttg
     3721 tgggaagagg cggggcgcat atccgccggc ttcgcgatgc acgccttcat cgtggccgcg
     3781 gacgtcggcc gggcccggca ggacgaccgg ctcaggagcc gagccgtggc caccctgctg
     3841 ggtatcggtg agcagttccc gcccggccag ctcttccgtc gcctgaccgc gttcgccgag
     3901 ccggatcccg aggccctcgt ccggaacgtg gtcctcgact ggcagccgta catcacccga
     3961 gtgcattacg tcgaacgggc cgtcgccctc tgcgccgacg tgcgccaccc gatcccgatc
     4021 gatggtctcg acacgctgct ggccgagtcc cgaacccgcg accagcgact cctgaccgcg
     4081 cagctcctcc gtgcgcgcgg gaaccaggag cgcgacgagg gcaatgtgcg cgaggcgctc
     4141 gagctgttcc gcgggtttgg tgcgggtccg ctcgtggccc gctgcgagat cgagctcggg
     4201 cagctcgtcg atgatccgga cctcgtcacc tcgggaacgg ccgtcctcga tgctctcggg
     4261 gacatgaccc acctgagccg ggtcgcggct cttcggcgtt gaacggtatg acccctagag
     4321 gcagctgaag cgggcgtccg cccagtcggc gtggtcgtag ttcttgctcg gcccggtggg
     4381 aacgatcagg ctgaggagac tcgtcccggc cggtagggcg agattgagac tgcgggtcgc
     4441 gctcgtgccg gtcatcgtcg ggctggtgta gatcgtggcg ccgttcgcct ggacctggaa
     4501 cgtgaggctg ccgaggctgc cgacctcgtc atcgagcccg accgaggcca ggaaggagca
     4561 gctcccggcc ggcaccgtgt agcgcaggtc ggaggcggca tgggttccca ggccccgcgc
     4621 gtacaccacg ccggccaggg tgatcggccc cccatcgccg gccaggtcct cgccgttgct
     4681 ccgatcgcgc tcatagggtc cccatccgtt cgtggccgtg gtcggggtca gggcactgag
     4741 gtaggtcacc gtcggcccgc cgctggcgac cgtgaaggtc gcggtgtagg tggcggaggt
     4801 cgcgggggcg acgatcgtgt ggctcgccgc gccgccatcg gaccacgtgc tgaactggta
     4861 gttcgtccca cccatgctct gcggcgacga tgcgctgacg gaggtcgccg agcccacgat
     4921 cacggttcgg gtgaaggggg tcgcctggct gctcgcgcca accgtcagtt ggaggccgct
     4981 tggactgctc gcaaagctca ggttcaccgt gcgtgggtcc agtcggcggg tcacggtcgt
     5041 gctcaggcca ccggaatcgg tcgcggtcag ctggaggtcc aggtatgacg ggtaatcgtg
     5101 atcgggtgcg gcaaacgagg agccagccgt tcccgaccag gtctggatgt catggctatg
     5161 gcagttcgac gggcagtgct ggacgatcag cttccagctg aggcgggcgg cagccaacgt
     5221 gccgtcctgg gcatcggtcg cgtgaccggt gaaagcgacc gactgaccga ccgcccatgt
     5281 ggtgccgacg gttggggtgt cgatcaccgg ctgcgggggc gtattcgacg agcctcccgc
     5341 accgcagctg aagcgagcgt cggcccagtc gccgtggtcg tggttcttgc tcggtccggg
     5401 ggggacgttc aatcggagga ggcttgcgcc ggcaggcagg accaggctga gggcgcgggt
     5461 ggcactggcg ccggtcatgg tcggactggt gtagagcgtc gtgctgtcgg cctggacctg
     5521 gaatgtcagg ctgccctggc tgccgacctc gtcgtcgaga ccgatcgagg ccagaaatgt
     5581 gcagttcgcg gccggcaccg tgtagcgcag gtccgaagcg gcatgggttc ccaggccgcg
     5641 ggcgtacacc gccccggcca gcgtgatcgg gcccccgtct ccagcgagcg cctcgccgtt
     5701 gctccgatcg cgctcatacg gaccccatcc gttggtcgcg acagtcggtg tcagcgcact
     5761 caggtaggtg acggtcgggc cgccactggt ggcggtcacc ggggtcgttg ccgtacccgt
     5821 cccaccgtgg ccgtccgaga cccgcagggc gacggtgacc gtccccaggg catacgtcct
     5881 cgacgcggtg gatccggttc cgtcgtcgaa ctggccgtcc ccgtcaagat cccacgcata
     5941 ggtcagcgga tcgccgttcg ggtcactcga ccccgccgcg ctgaaattca cgctcaacgg
     6001 ggccggcccg ctgctgggca ccgcggagac gaacgcggtc ggcgcgccat tgccggcatg
     6061 cgagatccgg cggatcgtcc cgccatcaaa gtccacgtag aacaagtctc cgccgggccc
     6121 gacctcgaga tccaccggat tcgccgctcc cgacacgaac gtcgcccggg tggtgggatc
     6181 cggcagaccg ttcgccccgg cgtacatcac ccagatgcag tcacgcgggt tgtccgcgaa
     6241 gaacagggcg ccggtgtact ggctcggata tgagccggga gctgagaaag ccatgccggc
     6301 ggtggcggag ctgccaaccg cgcagttttc accgttgacc accttggccg cgtggttata
     6361 ggcgaagtac ggagccacga ccgccccggc gcccgccgcg taaaggttct cgcagatgtt
     6421 gagattcgcc gcgtcgtatc cggcctggcg cccgcttccc tcgtagcagg gccagccgaa
     6481 gttcgcgacg gtgcccgacg gcacgaagcg gttgatctcc tcgaaggcgt cccagccgac
     6541 gtcgccgatc cacagctcgt tggtgccggg ccggacggtg aaccggaacg gattacggaa
     6601 tccctcggcg atgatccggc gcgcgttgac gtcggagctg ccggcgagcg gattctgcgg
     6661 cgcgccgagg ccggtggccg ggtcgaggcg gaggaccgca ccgctcagcg cgaccgggtc
     6721 gccgcccgtg cgcaaatcct gggaccgcag ggcgccacct tctgccgtgg gcgccgtctg
     6781 gttgccccca accgggactg gaggatcccc gcagggattc tcgggagtgg ggctgttacc
     6841 ggatcccccg ccctgcccgt agtcggcgaa cgtccagctc gcgccgtcac ctcccgacac
     6901 gtagaggtag ccgtcggctc cgaactcgag ggagccaacc gaatggctcg gatactgctg
     6961 gcaccaatcg gtgatcaaca ccgcttccgg accggcctgg ttgccgttcg cggtgagacg
     7021 cgacacacga cccgtgacca cgcaaccgtc ggtggtcggg ccggggggcg acgggcagcc
     7081 gtcggacgtc acgccgggcg atccccaatg gggggccgtg ccgccgggaa gggcgtcgta
     7141 ggcgtagagc acgtagacgc tcgggtcgga cgggaaggtc ggcgcgagcg ccattcccat
     7201 caatccgcgg tcccagaagt tgaagacgtt cgtgcgcagg tccgcgaaca cggtcggggt
     7261 cgggtcggcg agcgagtcga acaccttgat aaggccgctc ttttccccga cgaagacccg
     7321 gccatcggac gagaagcgaa tgaccatcgg gttggtcaga ccgctgaagg cgatggactc
     7381 cgaaaagccg gccatcgtgg cagcgcggga aaccggcggc gtgaacagct gtggcaccca
     7441 caacccgaca cccagaacca gggcgaggaa caggtggatg agacgtctgc tgaccatgcc
     7501 gggcacgtgg cgactccgtg actcgacggg tcgggctcgc cactcgctcg acgcgatcct
     7561 ggcgaccgtg ggcagatcgc gcgtcagcac cccgtccgac tcccgaccgt acacgcccca
     7621 ctgccgttgg caagggcgcg gcggccttct acggcagatt ccggtgactt caggcaccag
     7681 aaactcaaat ccggacgggt atcgtggccc gatggcagag gcaggtaggt atgacgtggt
     7741 cgtgatcggc ggcgggcttc ttggcctggc cacggcgcga aaccttctta ccgatggacc
     7801 gcctggcctc cggattgccg tcctggagca ggagagcgac atcgcgctcc atcagagcgg
     7861 acgcaactcg ggcgtgatcc accgtgggat ctactacgcc ccggggtctc tgaaggctcg
     7921 cctgtgcacg acgggatcgc ggcgactgac ggcgtattgc gacgagaagg ggatcccgtg
     7981 ggaacggacc ggcaaggtca tcgttgccct gaacgagcag gagctcccgc gcctggacga
     8041 gctgtatcga cgcggggtgg caaatggcgt ccctggcctc aaggtcatcg ggccggagca
     8101 cttgcgagag ctcgagccgt ccgccgtcgg cgtccgggcc gtccattcgc cggagaccgg
     8161 catcgtggac ttccggcggg tcgcgcaggc gtatgccgat gacgtggtcc acgccggagg
     8221 cgagatccgg ctggagtcgc gtgtgcgctc catcgaggag cggggcgacg aggttcgggt
     8281 gatgacggac ggcggcgaaa tcgggacgcg gtacctgatc gcctgcgcgg gactccaatc
     8341 cgaccgcgtt gcggcgatga cggacgcctc cgtgcgccgg gcaatgcgca ttctcccgtt
     8401 tcggggggac tacttcgtcc tgcgacccga acgatccttc ctgtgccgct cgctgatcta
     8461 ccccgtcccg gatctgcgct tcccgttcct cggggtccat ttcaccaagc gcatggacgg
     8521 ctcgatctgg gccggcccga atgccgtgct cgccctggcc cgcgagcggt acgggcgcgc
     8581 tgacgtacga atcgcagacg tcgtcgacac ggtgacgttc gcagggttct ggcatctcgc
     8641 catggcccac tggcgaatgg gcatgagcga ggcgtggcgc gacgtggccc gacacgcctt
     8701 cggtgatgcg ctccgtcgct ttgtcccgga ccttcgcgac gacgacctgc tgccggggcc
     8761 ggccgggaac cgggcgcagg cggtcgcggc cgacggctcg ctggtcgatg acttccggat
     8821 caccgcgtcc ggcaggagca tccatgttcg caacgccccg tcaccaggcg ccacctcttc
     8881 gttggcgatc ggagagatga tcgccaggca cgcgcgccgc cggttcacgc tggacgcctg
     8941 agcggtctgc gaggagaacg gacgaccgcc ggccgctcag cggccggcgg tcacctggtc
     9001 cgaggttgac ctcgggcgtt cgccttatgg aagtccggcg tggacctcca gcagatcgaa
     9061 ggtggcggtg tacggcgagg cgggtccctg cgaggtcgag aagatcccga ccgcgagggc
     9121 ctgcgtcccg tcggtccacg aggcaggcac ggttcgggca ccggctccgg tcgtgaatcc
     9181 actccgggtg acgccgttgt tggtcgagta cgtcgggctg taggtatgcg cgacggtgtc
     9241 gatgtacagg tacaggtcgg tgcatgccgc cgatccgatg gccgcatcca gcttcgcggc
     9301 aatgatcgag ccttcgccgt tgacctcccg actgtcatgg actccggtgc cggtggacgt
     9361 ctcgatcacg gcctttatgt agttgtcctg gttgcccggc ccgatgatga tcccggccga
     9421 ggcgaagacg cccggcgggt acgggctgca gacgcggccg tggaccgtga agctcgagac
     9481 cggcatggtc acgttgacct ggaagccgtt cttgaggtca ttcccaccat tcgccgagtc
     9541 gccttccggc acctggtcga cgacgaagtg tccggagcca tccagcccga ccagcgccgg
     9601 gttgtactgg gtgagcgacg gggtgaagga gttggtcatc aggccggtga acccaccgtc
     9661 ctcgatttcg cccccacctg cgtcgaaggt cagggagaac gggagcgtcc tgccgctcgc
     9721 gttggtgcca tcggtggcga aggcatcaca ttcatccggc tggccgtcgc cgtcggtgtc
     9781 gccgctgttg gcgatgaccg tcaggttgac cgttgccgtg tcgctccctc cggcgccatc
     9841 actgatggtg tagttgaagc tgtcggcgcc ggtgtacccg gaatcggaga cgtacgagac
     9901 gacgcgggga atctccacgc caccgaggcg gtgggtgagg atcccggccg taccgtgcgc
     9961 cgggtcggag accgccgtga tcgaaagggt tgctccctcg gcatcggtgt cgttgttgag
    10021 cacgcggtac tgccacttgc agtctgactt cacgttgccg aggttgtcgt tgttggcgtt
    10081 cgggggcatg ccaggaatga atgcctggag ctggctctcg agcgcggcgc agaccacccc
    10141 ggtcccgccg gcgatgatga tctggccagg gttgagccga tcgagctcgg tcgccgtctc
    10201 gctcggaatc gcgcactgcg agacgagcag gagcggacca cccaggtgcg cggcggcagg
    10261 gcctgctgcc agcgcatcag ggaatcccag accgttggcg acatagacga cgttcacgcc
    10321 cgggtcgaac gtgcccgccg aaatggcggc cgcggtcgcg tatcggttga gccctgcctg
    10381 gcggttgacc gtcgaggagt aggcacccaa ggcggtcttg acggcgtccg acacgacgcc
    10441 gatgccaccg gcgacgatga tctcgtcggg gttcagccgg tcgagctcgg ttgccgtctc
    10501 gctcgggatc gaggactggg tgacgaggag gagcggacca ccgagttcag ccgcggcagc
    10561 gccggcgccc agggcatcgg cgaatccaag gccgttgacc acgtaggcca cgttcacccc
    10621 agggctgaac gtgtcatggg agatggcggc cgccgttgca tagcggttga ttcccgactg
    10681 ccgggaaacg tctcccggag ccgcggcaac gctcagcgcc ggaagcgtgg acgcgatgac
    10741 gagggcacag agcgaaagcg aaattcggga aaacgtgcgt ggagccaaca gggatctccc
    10801 ctctcaggaa cgcggcgtgg gcatgcgccg gttgggatgg atgaggacgg attgaatgac
    10861 ggatctggac gatgccgctg gatggttccc ttggttccct cctgcgaggc agccagccca
    10921 aagccgcagg cgggacggta ctggttatgc gcttccgtgt ctaggacctt cggcggtcac
    10981 cgggcatttc gcagtgcctc cggtcaacac tttccctgga tcaggcgccg cccggcagcg
    11041 gtacatcctc gggaacgagg ccctcgctcc gcagctcgcg ccacaggtcg tccgggatgg
    11101 tccgcgtgaa gtgcgcaacg ttctccttga tctcgtctgc cgagcggacc ccgaccagca
    11161 cggacccgat ggcggggtga gcgaggggaa actggagcgc cgcggcggca agtggaacgt
    11221 cgtaccgcgc acacacggcc tgcatcttga gggcccgttg gagcggttcg gcgtcgctcc
    11281 ccacggcgtg gtagtcgaac gacgcccccg gcttgggatt ggccaggatc ccgctgttga
    11341 agacgccgcc aaggttgacg gtcatgcccc ggcgaagtgc ggttggcagg agatcacgaa
    11401 ggccggactg attgaggagg gtgtatcggc cggccagcag gaagcagtcg aaatcggcgt
    11461 cctccgcgaa gcgggtgagc atctgggact ggttcatgcc gaccccgatg gcttgaaccg
    11521 agccgtcccc ccggagccga tcgagtgccc tgaatgcccc ggacagggcc ttgtcgtagt
    11581 ggttgtcggg gtcgtggatg tacagcatgt caagccgatc gagaccgagc cgggtgaggc
    11641 tctcctcgac cgagcgcatg acgccgtcgt agctgaaatc gaaatacgaa tcgagcgctc
    11701 gctcgtcgcc gcggtcgaag gggaagccca gcggataggt cggatcgcgc ccgaggatgc
    11761 gccggccgaa gcgagctgca tggcgaccga tcagggccgg cccgcgctcc gggccgcgca
    11821 ccgactggtt gagaacgctg gcgaccttcc gggcggtgga gacgcggcgg agcaggcggc
    11881 cgactttcgt ggcgatcacg agttcgtccc gggggatcat ggatatggcc tcccccaccc
    11941 gcttctcggc caggccagcg ccatattggg gggcggtgtc gatcagccgc acaccgagcc
    12001 gatgggccgt ctcgacgatg ctgcgggcct cgtcatcctc gatcagaccc atcatgttcc
    12061 cgagcggaac cgtgccaagg ccgagtcgtg agatacggag gctggtcgag ccgacttgcg
    12121 cggttgggag gtcttccggc tcatgtgact gggtgctcat aggcgatacc tcgaaatgtc
    12181 ggtcagtcct gtagtcagga ggtcaacgga tatctcgccg gccacgatgg cgaggacggg
    12241 cagcagggcg acgagaataa gggcagctcg cgccggccgc tcatagggaa tcagcgcgat
    12301 cgcgcccacc gtcagcccca cgagcgcgag catcacgagc tcgggggacc agacgcccag
    12361 gtagaccgtg atcgcgacaa caagggcatc gatggacagg ctcaccggga cgacgagcgc
    12421 aacccgagcc actcggtccg tgatgccggt cagccacagg actgccagcc ccgggccaat
    12481 ggccaggaag acggcgaccg tgatctcgcg gagcggcagc tcgtcggccg cgatggcgaa
    12541 gctcgcggca aggcccagca acgcgaccag gaggatctgg agtctggcgg gggaggtcat
    12601 cggtccggct cggccggctc ggccggcggc ggcacgcgat agaccgcccc atcggcattc
    12661 gcgaagagga gctctgcccg ctgtgccatc gcctggaccc gatgatccca tcgggcatcg
    12721 ctcatgttcc agaagagctc cgcatacgcg cgctggccgc gggtgatgat gaggtagctg
    12781 tcattcgcct ccggagtgcg gcgcgtgagc tcgagcacga cgagatccat gtcgccgaat
    12841 cgccactcgg agtcgagggc gacgtgccgg tgctgctccc actcgatgta ccccagcggt
    12901 acggagtgat tcgccgagcc gatggtgtcc ccgggctgcg ctaccgatgc cacgtattgc
    12961 atgacacggt attccgccgc cgtcacgtag tccgccttct cgttcccata gcgggtcagg
    13021 agcatgacgc tggccagggt gatggacagg agcccgaggg cgccgaacgt gcgccacgtc
    13081 ccgggtcgca tggtcggaaa gaacgcggcc gccgcgaaga acgccatgaa cggacccgag
    13141 aacaggtaga cccgcagcag catctcgccg ccgtacgcct ggagcaggat cagaccgaag
    13201 ggtgcaatgg cgagcacagc cagcgtcaga tccagctggc cggaccgcag ccggcgcagg
    13261 ccgccgatca gggccaggac ccagacaccg agggtcagga cgagtcgcgc ctgcagcacg
    13321 aacaggtggc cccggctgcc aaccagtcga gccaaggtcg aatcgatgtt ctcgggtcgg
    13381 aagatctgtt cgaggaggct cgcaagatgg cccgatagga aagtcgtggc catgaacatc
    13441 agccagaggc tcagcaggac gaccatgaac atggccagac cggtcaggcg aatccggttg
    13501 aagatggtca agccgatgac gccgccaacg agggcgaacg gcgtcagctg atgactcgca
    13561 acggacgttc cgaacatcgc cgcgacgatg gcgatgagcc caatctgctg gcggaccgag
    13621 atcgagcgcc cggcgcgaac ggcctcgatc gagcccgctg ggtcgaccag gatcgcggcc
    13681 agcagggacc ggagccggag gcgcgccgac gacgtcccac cgccggtcga gcctgctgac
    13741 ccgctccgac ggaaccaggt cagcaggacg gccagtacgg ccagcagcag gagcaggttg
    13801 aatgcctggg gcgagaagta gtcctggccg acccagttga cgaggt
//
LOCUS       DASYGU010000296         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_306347, whole genome shotgun sequence.
ACCESSION   DASYGU010000296 DASYGU010000000
VERSION     DASYGU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306347"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VGO32_08500"
     CDS             complement(<1..833)
                     /locus_tag="VGO32_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7200826.1"
                     /translation="MGISLRFLGAAGGVTGSQFLLTVGSRRVLVDCGLFQGGMEEIAR
                     NRMAFSFGADDIHAVLLTHAHNDHIGRLPALVRAGYRAPVFATDATADLAEIVLTDSA
                     HLQARAAERWARRHPEPAAATAGESESTVTETEMAAVAAQVPKPPPMDTTTHAALFDE
                     ADVRRTVELVRPVSYDATTQVADGVEATFYDAGHILGSAIIELRVREPGGAEMTFVFS
                     GDLGRPDTPILRDPTPLTHADVVIVESTYGDREHPAQEQAIEELVTVVTEVAKSGGVL
                     L"
     gene            872..1957
                     /locus_tag="VGO32_08505"
     CDS             872..1957
                     /locus_tag="VGO32_08505"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7200827.1"
                     /translation="MAFPIEPPIEPMLAKPQPEIPRGDGWRYEPKWDGFRAIVFRDGP
                     DVYIQSRDLKPLNRYFPELEEPLRKIGRPPDRFVLDGEIVIATDDGLDFDSLLIRIHP
                     AASRVAMLAEASPASYVAFDLLGRGDRDLRETALAKRREALERMLAKPPPSVLLTPAT
                     DDPETANRWFADFEGAGLDGVVAKRADGHYVPGKREMIKVKHQRTADCVVAGFRWHKN
                     GPGTMVGSLLLGLYDGAGTLQSVGVTSSFTETRRRELVEELKPLRKDALKNHPWQEWK
                     EAEAAWAGQRMPGATSRWNRGKDLSWEPLRIERVVEVAYDHLQRDRFRHATTFLRWRP
                     DRKPASCRYDQLEETAPALLADLFASH"
     gene            2034..>3067
                     /locus_tag="VGO32_08510"
     CDS             2034..>3067
                     /locus_tag="VGO32_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7200828.1"
                     /translation="MSHHDDPNAAVADLADRFWEGVLERDPITATIYGDDRYDERWPD
                     LGPDGRAAERSALSSALAEAEAIDPDALEVESVITRDLLILIARNYLEAQDRKLYQLA
                     VNHMSGPQTWPSEIAQYQRADTPESLDRMLARFAAFPTLIEQQIGTLDEGIADGRTSA
                     AVPVQKAIEQIDRMLTVPSDIAPAVVLANVADESGREAIRGSVVQDIYPALKRLRDYL
                     AETYTPHAAPTPGIGDTPGGHDAYGLAIRMQTTVDATPEEVHAFGMEDLERIEAEKDE
                     IARRQGHADRHAFVAALAEDPSNRTDDPDHIIALAREQTDRAYATAPQFFGRLPAANC
                     TVLAVEEYRER"
BASE COUNT          493 a         1105 c         1030 g          439 t
ORIGIN      
        1 atgaggagca cgcccccgct cttcgccacc tcggtcacga ccgtgaccag ctcctcgatg
       61 gcctgctcct gggcggggtg ctcgcggtcc ccgtacgtcg actccacgat caccacgtcg
      121 gcatgggtca gcggcgtcgg atcgcggagg atgggggtgt ccggccgacc caggtcgccc
      181 gagaacacga atgtcatctc cgcgccgccc ggctcacgga cgcgaagctc gatgatcgcc
      241 gagcccagga tgtggccggc gtcgtagaag gtggcctcga cgccgtcggc gacctgggtc
      301 gtggcgtcgt acgacaccgg ccgaaccagc tcgaccgtcc gccggacgtc agcctcgtcg
      361 aacagcgcgg catgggtggt cgtgtccatc ggcggcggtt tcggcacctg ggcggccacg
      421 gccgccatct cggtctcggt caccgtggat tccgattcgc ccgcggtggc ggccgctggc
      481 tccggatgcc gtcgcgccca tcgttcggcc gcccgcgcct gcaggtgggc gctgtcggtc
      541 agcacgatct cggcaaggtc ggcggtggca tcggtcgcga acacgggcgc gcggtatccg
      601 gctcggacaa gcgccggcag ccggccgatg tggtcgttgt gggcgtgggt cagcagcacc
      661 gcatggatgt catcggcccc gaacgagaac gccatccggt tgcgggcgat ctcctccatc
      721 ccgccctgga acagcccgca gtcgacgagg acacgccgcg agccgacggt gagcaggaac
      781 tgcgaaccgg tgacgccacc ggccgcaccc aggaatcgga gggagatgcc caccggtcga
      841 gtatgcggca ggcgcgggct actatcggtc cgtggccttc ccgatcgaac ctcccatcga
      901 gccgatgctg gccaagccgc agcccgagat cccgcgtggc gacggctggc gctacgagcc
      961 caagtgggac ggcttccgcg ccatcgtctt tcgcgacggg ccggatgtgt acatccagag
     1021 ccgcgacctc aagcccctga accggtactt ccccgagctc gaggagccgc tccgcaagat
     1081 cggccgaccc cccgaccgtt tcgtcctgga tggagagatc gtcatcgcca ccgacgatgg
     1141 tctggatttc gattcgctcc tgatccgcat ccatccggcg gcttcgcggg tcgcgatgct
     1201 ggccgaggca agccccgcct cgtacgtcgc gttcgatctc ctgggtcgtg gcgatcgcga
     1261 cctgcgcgaa accgcgttgg ccaagcggcg cgaggcgctc gagcggatgc tggccaagcc
     1321 gccgccatcg gtcctcctga ccccggccac cgacgatccg gaaacggcca accgctggtt
     1381 cgccgacttc gagggggccg gcctcgacgg ggtcgtcgcc aagcgggccg atgggcacta
     1441 cgtgccgggc aagcgggaga tgatcaaggt caagcaccag cgaaccgccg actgcgtggt
     1501 cgccggcttc cggtggcaca agaacgggcc gggcacgatg gtgggctcac tcctgctggg
     1561 tctgtacgac ggcgccggca cgctccagag cgtcggcgtg acgagctcgt tcaccgagac
     1621 acgccggcgc gagctcgtcg aggagctcaa gccgttgcgg aaggacgccc tgaagaacca
     1681 cccgtggcag gagtggaagg aggccgaggc cgcctgggct gggcagcgaa tgcccggcgc
     1741 gacgagtcgc tggaaccggg gcaaggacct gtcctgggag cccctccgca tcgagcgggt
     1801 ggtggaggtg gcgtacgacc acctccagcg cgaccgattc aggcacgcga ccacgtttct
     1861 ccggtggcgg cccgaccgca agccggcgtc gtgtcgctat gaccagctcg aggagacggc
     1921 tcccgcgctg ctggccgatc tgttcgcgag ccactgaccg ggatacactc gccgggcact
     1981 catcccgtca acctgaccac accattccga cacccccaag gagactgtgc ctcgtgtccc
     2041 atcacgacga ccccaacgct gccgttgccg acctcgccga tcgtttttgg gagggcgtcc
     2101 tcgagcgcga cccgattacc gccacgatct acggcgacga ccggtacgac gagcgctggc
     2161 ccgacctcgg tcccgacggc cgagccgccg agcggagcgc cctgtcctcc gcgctcgccg
     2221 aggcggaggc gatcgacccc gacgcgctcg aggtcgaatc ggtcatcact cgcgacctcc
     2281 tgatcctcat cgcccgaaac tacctcgagg cgcaggaccg caagctgtac cagctggccg
     2341 tcaaccacat gtccgggccc cagacctggc cgtcggagat cgcccagtat cagcgcgcgg
     2401 acacgcccga gagcctcgac cggatgctgg ctcgctttgc cgcgttcccg acgctcatcg
     2461 aacagcagat cggcaccctg gacgagggga tcgcggacgg tcgaacctcg gccgcggtcc
     2521 cggtccagaa ggcgatcgag cagatcgacc ggatgctgac cgttccttcc gacatcgccc
     2581 cggctgtcgt cctggccaac gtggccgacg agtccggccg cgaggccatt cggggctcgg
     2641 tggtgcagga catctatccc gccctgaagc gtctgcgcga ctacctggcc gagacctaca
     2701 ccccgcacgc cgcccccacg ccgggcattg gcgacacgcc cggcggccac gacgcgtatg
     2761 ggcttgcgat tcggatgcag acgacggtcg acgccacgcc ggaagaggtc catgccttcg
     2821 ggatggagga cctggagcgg atcgaggccg agaaggacga gatcgcccgc cgccaggggc
     2881 acgccgaccg gcacgcgttc gtcgccgccc tggccgagga tccatccaac cggaccgatg
     2941 acccggacca catcatcgcg ctggcccgcg agcagaccga ccgggcctac gccaccgcgc
     3001 cgcagttctt cggtcgcctg ccggcggcca actgcaccgt cctggccgtc gaggagtacc
     3061 gcgaacg
//
LOCUS       DASYGU010000297         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1078517, whole genome shotgun sequence.
ACCESSION   DASYGU010000297 DASYGU010000000
VERSION     DASYGU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078517"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VGO32_08515"
     CDS             <1..810
                     /locus_tag="VGO32_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7200829.1"
                     /translation="LGIGMRKPRNGGLGLDVAGVVEAVGPEVGRFRPGDQVFGDMTQF
                     GHGAYAELVTAPAHAFAPVPAGVSLEDAATTPQAAILAIQGLDAQGGIKPGDKVLING
                     ASGSVGPFAVQIAKSRGAEVTGVASTKKLEFVRSLGADHVLDYTRDDYTELGQRYDRI
                     LDMAAYHSIGDIRRVLTRTGGYTAIGGSLVNVFGAMLVGVPMRLAGKQRAGLPMWKPF
                     RAEDVVVVSRMLADGTIKPIIDKRFPLAEVPDALRYADDGHARGKVVITVP"
     gene            871..1140
                     /locus_tag="VGO32_08520"
     CDS             871..1140
                     /locus_tag="VGO32_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200830.1"
                     /translation="MAARVKRTYNLDPRTVHAVRELADRYGVASSQDAVVELAVDELR
                     RLVAERDESAAWERASTDPEWLAESRDLEEAYRSADRETWPADPS"
     gene            1119..1511
                     /locus_tag="VGO32_08525"
     CDS             1119..1511
                     /locus_tag="VGO32_08525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV7200831.1"
                     /translation="MAGRPVLIDRLRWATVIADLDPVIGHEQAGQRRALVVSYEPFHR
                     SGLMTVCPITAARAEPRYPGDVAIPAGHAGQTRDGVIVCSQVRTISSDRVQIPPVGYL
                     TDPDRRCQVRHALAHHLGLDVPAVADGA"
     gene            1577..2017
                     /locus_tag="VGO32_08530"
     CDS             1577..2017
                     /locus_tag="VGO32_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200832.1"
                     /translation="MSSTTSFAATSSPNTSQPSLAPAEYQAGVCNIGPEEIARRRRAG
                     HFGAAITVGLLIGLIALGAPPLLRLVVFLPAAAAASGYLQAWLKFCAGFGRLGVYNFG
                     RVGPMTPVTDDASRAADRRKATQIGVASGLIGLVVAVIAVLLPV"
     gene            2014..>2559
                     /locus_tag="VGO32_08535"
     CDS             2014..>2559
                     /locus_tag="VGO32_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEV7200833.1"
                     /translation="MTDPVEQVAAALRSRNIEAVVVDTPEEARAAVLALVPDGAEVHT
                     AKSKTLADVDLSEELSESGRYDAIRPRLLTMDRDTQGREMRKLLAAPDFMVGSVQALT
                     IDGTLVSASATGSQLGPYVSGAGRVILVVGSQKIVPDFEAAIRRINEVVFPYENALVQ
                     AKMGVDTRLEKLLVWYGEWRPG"
BASE COUNT          377 a          874 c          898 g          410 t
ORIGIN      
        1 ctggggatcg ggatgcggaa gccgcggaac gggggactcg gactggacgt tgccggagtt
       61 gtcgaggcgg tcggtccaga ggtcggtcgc ttcaggcccg gcgaccaggt gttcggggac
      121 atgacccagt tcgggcacgg cgcatacgcg gagctcgtca ccgcgccggc ccatgccttc
      181 gccccggtgc cagccggcgt ctcgcttgaa gatgccgcca ccaccccaca ggcggcgatc
      241 ctggccatcc agggcctgga cgcccagggc gggatcaagc cgggcgacaa ggtgctcatc
      301 aacggcgcgt cgggcagcgt ggggccgttc gcggttcaga tcgcgaagtc gcgcggggcg
      361 gaggtgaccg gcgtggccag caccaagaag ctggagttcg ttcggtccct gggcgccgac
      421 cacgtcctgg actacacccg cgacgactac accgagctgg gccagcggta cgaccggatc
      481 ctggacatgg cggcgtacca ctcgatcggc gacatccgcc gggtcctgac ccggaccggg
      541 ggatacaccg cgatcggcgg gtcgctcgtg aatgtcttcg gcgccatgct ggtcggggtg
      601 ccgatgcggc tggccggcaa gcagcgggcc gggctgccga tgtggaagcc gttccgggcg
      661 gaggacgtgg tggtcgtgtc ccggatgctg gccgatggga cgatcaaacc gatcatcgac
      721 aagcggttcc cgctggctga ggtgcccgat gcgctgcgct acgcggacga cggccatgcc
      781 cgcggaaagg tggtcatcac cgttccgtag catctctgca gccgatggac ccagattgac
      841 acccacgatg acacgtgtca gcatccgtcc gtggccgcca gggtgaaacg cacctacaac
      901 ctcgatccgc ggaccgtcca tgccgtgcgg gagctggcgg atcgatatgg cgtcgccagc
      961 tcgcaggacg ccgtcgtgga gctggcggtg gacgagctgc ggcgcctggt tgccgaacgg
     1021 gacgagtccg cggcctggga gcgggccagc acggatccgg agtggctggc cgaatcgcgg
     1081 gacctggagg aggcctaccg gagcgccgat cgcgaaacgt ggccggccga cccgtcctga
     1141 tcgaccggct gcgctgggcg accgtcatcg ccgaccttga tccggttatc ggccatgagc
     1201 aggctgggca gcgccgggcg ctggtcgtct catacgagcc gtttcaccgc tccgggctca
     1261 tgaccgtctg ccccatcacc gccgctcgag ccgagccgcg gtacccgggc gatgttgcca
     1321 tcccggccgg acatgctgga cagacccgcg acggcgtcat cgtgtgcagc caggtccgaa
     1381 ccatctcctc ggaccgggtc cagattcccc ccgtgggcta cctgaccgat cccgaccgcc
     1441 ggtgccaggt gcgccatgcg ctggcccatc acctcgggct ggacgttccc gccgtggcgg
     1501 acggcgcctg acacccagaa cgcgcgctca ttcgttcggt cctagacttg cccggtcaca
     1561 tccggggaga ttcaccttgt cttcgaccac gtcctttgct gccaccagca gccccaacac
     1621 ctcgcaacct tctctggctc ccgccgagta ccaggcaggc gtctgcaaca tcggtcctga
     1681 agagattgcc cgtcgacggc gggccggcca tttcggcgcc gcgatcaccg tgggcttgct
     1741 cattgggctc attgccctcg gcgcgccgcc gctcctgcgc ctggttgtgt tcctgccggc
     1801 tgccgccgcc gcgtccggat acctgcaggc gtggctcaag ttctgcgccg ggttcgggcg
     1861 gcttggcgtc tacaacttcg ggcgcgtcgg cccgatgact cccgttactg acgacgcctc
     1921 gcgagccgcg gaccgccgga aggcgaccca gatcggggtt gcgtcggggc tcatcggtct
     1981 ggtggttgcc gtcatcgccg tcctgttgcc ggtgtgaccg acccggtcga gcaggtcgcg
     2041 gccgccctgc ggtcgcgcaa tatcgaggcg gttgtggtcg acacgcccga ggaggcgcgg
     2101 gccgccgtcc tggcgctcgt ccccgacggt gcggaggtcc ataccgccaa gtcgaagacc
     2161 ctcgccgacg tggacctgtc cgaggagctg agcgagtcgg ggcgctacga cgcgattcgg
     2221 ccgcggctat tgacgatgga ccgcgacacc cagggccggg agatgcgcaa gctcctggcg
     2281 gcaccggact tcatggtcgg cagcgtccag gcactcacga tcgatgggac gctggtctcc
     2341 gcctcggcca ccggcagcca gctcggaccg tacgtgtcag gcgctggtcg cgtgatcctg
     2401 gtcgtcggca gccagaagat cgtgccggat ttcgaggcgg ccatccggcg catcaacgag
     2461 gtcgtcttcc cgtacgagaa cgccctggtc caggccaaga tgggcgtgga cacgcgcctc
     2521 gagaagctcc tcgtctggta cggggagtgg cgccccggc
//
LOCUS       DASYGU010000298         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_616032, whole genome shotgun sequence.
ACCESSION   DASYGU010000298 DASYGU010000000
VERSION     DASYGU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616032"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VGO32_08540"
     CDS             complement(<1..423)
                     /locus_tag="VGO32_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEV7200834.1"
                     /translation="MRGASAERGQVLVIVALTITLLLAFAGLAIDVGRQVAEQRHIQT
                     AADAAALAGCRALIAGDSDASAAAQARAIGEVNLEHSPAAGLAVIAPDIARVYTSGHA
                     GDPAYLESGIIISGTTIRVAVHSEVEALLARVVGVDSLE"
     gene            complement(426..956)
                     /locus_tag="VGO32_08545"
     CDS             complement(426..956)
                     /locus_tag="VGO32_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEV7200835.1"
                     /translation="MVEFAAVLLPLILIVVGIIQFGLLYGAHVTLTNAAREGARAGTI
                     YVYDQNHTAYWNDAHRCGAILEAINASFGLLDETSPHFATTMSAGACATPTSTTLVNG
                     DLTVSYCATASGENPCPDPGNSLTNCVTDSREGCLVRVEIRYHSDIVVPLIGDLLATD
                     GGGRFLSRAMATMVVN"
     gene            1171..3309
                     /gene="uvrB"
                     /locus_tag="VGO32_08550"
     CDS             1171..3309
                     /gene="uvrB"
                     /locus_tag="VGO32_08550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEV7200836.1"
                     /translation="MADFTLHAPFEPTGDQPLAIAGLVDGLQAGMRHQVLLGATGTGK
                     TFTAAKVIEAVQRPTLVMAHNKTLAAQLYQEFREFFPDNAVEYFVSYYDYFQPEAYLP
                     RSDTYIEKDSSRNDEIDRLRHNATKALFERRDVIIVASVSCIYGLGAPIDYGATVINL
                     KVGGHYRRDGVLRHLVDLQYQRNDAALQRSRFRVRGDVLEVQPPHGEEVVRIDFFGDE
                     VERIAEVDGLTGEVLAERTEVNFYPATHYVTPRDKLLAAIGDIEVEMEIRVRELESRG
                     QALEAARLRQRTMFDLEMLREVGFCPGIENYSRHLSRREKGSRPWTLIDYFPTDFLLI
                     VDESHITIPQVHGMYKNDRTRKEVLVEFGFRLESALDNRPLTFDEWEAHLNQVVSMSA
                     TPGPYETERAERVVEQFIRPTGVVDPSITIRPTAHQVDDLLAEIRETVARGERVLVTT
                     LTKKMAEDLADYLAELGIKVQWLHSEVDTLERIEILRDLRLGVYDVLVGINLLREGLD
                     LPEVTLVAILDADKEGFLRSGGSLIQVIGRAARNIGGRVIMYADKETAAMRAAIEETD
                     RRREKQVAYNLEHGIEPETIIKGIRDIHEGLRRVAEERKTLDLEGRGSEEIAELAQRL
                     EAQMKEAARNLEFERAAALRDELVALRRLREELNAAPGEIPADVYAAAAEAGQDGWGG
                     DGFPRREVFSTAARPNIIPAGSRRRPPRRR"
     gene            3319..3924
                     /locus_tag="VGO32_08555"
     CDS             3319..3924
                     /locus_tag="VGO32_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200837.1"
                     /translation="MAGTLPHAVFVETDLDPSGTAAAFAGELPGCAAFGSGPAEAVAA
                     VPARVGAFVAWLRAHGEALTEPVGNWYEVERAPAAPAGESIRRATFSLDELPPSAGEL
                     ATWLHWADLAREDLADALDATPGAVLDATWIADQDLALAADLGARHAPPDGAPPLDRL
                     YAARDVLIEALGSAGLDGGVRRVIRLVIADDLRMTERLRDR"
     gene            3921..4460
                     /locus_tag="VGO32_08560"
     CDS             3921..4460
                     /locus_tag="VGO32_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200838.1"
                     /translation="MTRLIPRSERVAWIAVPIVLVIVLVVFLSIGSLGAPRAELERIN
                     VAEVLAVTDPATTYGDAEIEIVGWYASRAGDCEAPPDEPIAVTWLDRTCPLRLLLAEQ
                     PATGAAQVALEEIGLRLAAPSGEPFPPRSEPGGWHLMLEPLIVTGHFDDPASADCAPS
                     RTARCRATFVVTEVDGLVH"
BASE COUNT          712 a         1568 c         1665 g          678 t
ORIGIN      
        1 ctcgaggctg tcgaccccga ccacccgggc caggagggct tcgacctccg agtggaccgc
       61 gacccggatc gtggtgccgc tgatgatgat cccgctctcg aggtaggcgg gatccccggc
      121 atggccggag gtgtagaccc gcgcgatgtc tggggcgatc accgccaacc cggctgccgg
      181 ggaatgctcg agattcacct cgccgatcgc gcgcgcctgg gccgctgcgg aggcatccga
      241 atcgccggcg atgagggcgc ggcagccggc cagggcggcc gcatcggccg cggtctggat
      301 atgtcgctgc tccgcgacct gccgcccgac gtcaatggcc agccccgcga acgccaggag
      361 gagcgtgatc gtgagggcca cgatgacgag gacctggccg cgctccgcag acgcgccgcg
      421 catagtcagt tcacgaccat ggtggccatc gcccgactga ggaatcgacc gccgccgtcg
      481 gtcgcgagca gatcgccgat gagcgggacg acgatgtcgc tgtggtagcg gatctcgacc
      541 cggacgaggc acccttcccg cgagtcggtc acgcagttgg tgaggctgtt tcccggatcg
      601 gggcacgggt tctcgccgct cgccgtggcg caataggaga cggtcaggtc gccgttcacg
      661 agcgtggtgc tggtcggggt cgcgcaggca ccagcggaca tcgtggtggc gaagtgcggg
      721 cttgtctcgt cgaggagtcc gaacgaggcg ttgatggcct cgaggatcgc gccgcagcga
      781 tgtgcgtcat tccagtacgc ggtgtggttc tggtcgtaga cgtagatcgt ccccgcccgg
      841 gcgccctcgc gggcggcatt cgtgagcgtc acgtgcgcgc cgtacaggag gccgaactgg
      901 atgatgccga ccacgatcag gatcagcgga agcaggaccg cggcgaactc gaccatcgcc
      961 tggccacggg gcgcggcggg cgcggagagg cgacgggtga cgcgctgcgc ccgccacccg
     1021 gaccctgggc taaccccggc acggcgcatg gagccggaga ctaggtcgcg cagtgcgcgc
     1081 gggatatggg ccgttcgata gcctgccgat cggtaccgat ggagcctcgg ctagaatcgg
     1141 tgcccccgac gagggccctc cggttccgac atggccgatt tcacgcttca cgccccgttt
     1201 gagcccaccg gcgaccagcc gctggcgatt gccgggctgg tcgatgggct gcaggcgggc
     1261 atgcgccacc aggtcctgct cggcgcgacc gggacgggga agacgttcac cgcggccaag
     1321 gtcatcgagg ccgttcagcg accaacgctg gtgatggccc acaacaagac cctggccgcg
     1381 cagctgtacc aggagttccg cgagttcttc ccggacaacg cggtcgagta cttcgtcagc
     1441 tactacgact acttccagcc ggaggcctac ctgccgcggt cggacaccta catcgagaag
     1501 gactcgtccc ggaacgacga gatcgaccgg ctccggcaca acgcgaccaa ggccctgttc
     1561 gagcggcgcg acgtgatcat cgtggcttcg gtgtcatgca tctacggcct cggggcgccc
     1621 atcgactacg gggcgacggt gatcaacctg aaggtcggcg gccactaccg ccgagatggc
     1681 gtgctccgac acctcgtgga tctccagtac cagcgcaacg acgccgcgct ccagcgctcc
     1741 cggttccggg tccgcggcga cgtgctcgag gtccagccgc cgcacggcga ggaggtggtt
     1801 cggatcgact tcttcggcga cgaggtggag cggatcgcgg aagtggacgg gctgaccggc
     1861 gaggtgctcg ccgagcggac cgaagtgaac ttttacccgg ccacccacta cgtcaccccg
     1921 cgtgacaagt tgctggccgc gatcggcgac atcgaggtcg agatggagat ccgggtccgc
     1981 gagctcgaga gccgcggcca ggcgttggag gctgcgcgcc tccggcagcg gacgatgttc
     2041 gacctcgaga tgctccggga ggtcgggttc tgccccggca tcgagaacta ctcgcggcat
     2101 ctctcccgtc gggagaaggg cagccggccg tggaccctga tcgactactt cccgaccgac
     2161 ttcctgctca tcgtggacga gagccatatc acgatcccgc aggtccacgg catgtacaag
     2221 aacgaccgga cccggaagga agtattggtc gagttcggat tccggctcga atcggcgctc
     2281 gacaaccggc cgctgacctt cgacgagtgg gaggcgcacc tcaaccaggt ggtgtccatg
     2341 agcgcgacgc ccggcccata tgagaccgag cgtgccgagc gggtggtgga gcagttcatc
     2401 cggccgaccg gggtcgtgga cccgagcatc accatccggc ccaccgccca ccaggtcgac
     2461 gacctgctgg ccgagatacg cgagaccgtt gcccgcggcg agcgggtcct ggtcacgacc
     2521 ctcaccaaga agatggccga ggacctggcc gactacctcg ccgagctcgg gatcaaggtc
     2581 cagtggctcc acagcgaggt ggacaccctc gagcggatcg agatcctccg ggacctgcgc
     2641 ctgggggtct acgacgtgct ggtcgggatc aacctcctgc gcgagggcct cgatctgccg
     2701 gaggtcacgc tggtcgccat cctcgacgcc gacaaggagg ggttcctccg gagcggtgga
     2761 tcgctcatcc aggtcatcgg ccgcgcggcc cggaacatcg gtggccgggt gatcatgtac
     2821 gccgacaagg aaaccgccgc catgcgcgcg gccatcgagg agaccgatcg gcgccgagag
     2881 aagcaggtgg cctacaacct cgagcacggg atcgagcccg agacgatcat caagggcatc
     2941 cgcgacatcc acgagggact ccggcgcgtg gccgaggagc ggaagacgct cgacctcgag
     3001 gggcggggga gcgaggagat cgcggagctc gcgcagcgac tggaagccca gatgaaggag
     3061 gccgcccgga acctggagtt cgagcgggcg gccgcgctcc gtgacgagct cgtcgccctc
     3121 cgtcgccttc gcgaggagct gaacgcggcc ccgggtgaga tcccggcaga tgtctacgcc
     3181 gccgccgcgg aggccggtca ggatggctgg ggcggcgacg gcttcccgcg acgcgaggtg
     3241 ttcagcacgg cggcccgccc gaacatcatt ccggccgggt cgcggcgccg tccgccccgc
     3301 cgtcgctgag accggcgcat ggccgggacg ctgccccatg cggtcttcgt cgagaccgat
     3361 ctcgatccgt ccgggaccgc agccgcattc gcgggcgagc ttcccggctg cgccgcgttc
     3421 gggtcggggc cggccgaggc cgtggccgcg gtgccggctc gcgtcggggc cttcgtggcc
     3481 tggctgcgcg cccacggcga ggcgctgacc gagccggtgg ggaactggta cgaggtcgag
     3541 cgggccccgg ccgcgccggc cggggagagc attcgacggg cgaccttctc gctcgacgag
     3601 ctgccaccgt cggcgggcga gctggcgacc tggctccact gggcggacct cgcccgcgag
     3661 gacctggccg atgcgctgga cgcgactccc ggggccgtgc tggacgcgac ctggatcgcg
     3721 gaccaggacc tcgcgcttgc cgccgacctg ggcgcccgtc acgcaccgcc cgatggcgcg
     3781 ccgccgctcg atcggctgta cgccgcgcgt gacgtgctga tcgaggcgct gggatcggcg
     3841 gggctcgacg gcggcgtccg ccgcgtgatc cggcttgtga tcgcggacga cctgcggatg
     3901 acggagcgcc tccgcgaccg atgacccggc tgatcccgcg gtccgagcgc gtcgcgtgga
     3961 tcgccgtccc gatcgtgctc gtcattgtcc tggtcgtgtt cctctcgatc ggcagcctgg
     4021 gcgccccgcg cgccgagctg gagcggatca acgtcgccga ggtgctggcc gtgacggacc
     4081 cggcgacaac ctacggggat gccgagatcg agatcgtggg ctggtacgcg agtcgggccg
     4141 gggattgcga ggctccgccg gacgaaccga tcgcggtgac ctggctggat cggacatgcc
     4201 ctctccgcct cctgctcgct gagcaaccgg cgaccggcgc cgcgcaggtt gccctcgagg
     4261 agatcgggct ccggctggcc gcgccgagcg gggagccgtt cccaccccgc tccgagcccg
     4321 gcggctggca cctgatgctc gagccgctca tcgtgaccgg gcacttcgac gatcccgcgt
     4381 ccgccgactg cgccccgtcg cggacggctc gatgccgggc gacgttcgtc gtcaccgagg
     4441 tcgacggtct cgtgcactag ggggcttccc cgcacacggc ttaaggcgca agattgtcgg
     4501 cagccctacc agatccgggg ggctggacct gttgttggag ggtactcaat ggatttccag
     4561 gcgcttctcg tgggcatcat cgcgatcgcc ctgggagcgg cgatcatgac gctgggctat
     4621 cgc
//
LOCUS       DASYGU010000299         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_873358, whole genome shotgun sequence.
ACCESSION   DASYGU010000299 DASYGU010000000
VERSION     DASYGU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873358"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1552
                     /locus_tag="VGO32_08565"
     CDS             <1..>1552
                     /locus_tag="VGO32_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7200839.1"
                     /translation="VPGGLRGLPGRSAPLTGREEELTILRDTARRASNDRKALLFTIL
                     GQPGVGKSRLARELGIELEPAGWRVLYGRCLPYGEGITYWPLGEVIRDLAEITPEMNS
                     AVAQQRLAAVSPDRDTAERLAFAIGLRAEAPVSGESLDREIAYAVRRLVESPATDRPI
                     LLVVEDIHWAEPPLLDLLEYLATWTRDRCLLIVALARPDLIDRRPGWGSGRMEASRLQ
                     LEALSREEATSLVHALLAADGLPEDLRDRILDRAEGNPLFVEETIRMLIDQGAVVERD
                     GAWVAANELVEVEVPETIEALVRARIDALPRDDRSILQCAAVIGRTFRRSALAALVDG
                     PVDTFLDEAILRDLVSEEPAADPSYRFKHLVIRDVAYGSLPKARRAALHRRTVEWLIE
                     WAADRRDEFVEIEAQHLEQAVKLLNELEGQADGALVDQAVAALRRSTEKAGARDDLRA
                     VISFANRALALHPRSTEERLELQTMLLEAMFESGDFAHGRDLGAAVAEQAGAIGRRDL
                     RGRALLRAG"
BASE COUNT          223 a          560 c          549 g          220 t
ORIGIN      
        1 gtgcccggcg gcctccgtgg gctgcccggc cggtccgcgc cactgaccgg ccgcgaggag
       61 gagctcacca tcctccgcga cacggcgcgt cgggcgtcga acgatcgcaa ggcgctcctg
      121 ttcacgatcc tgggccagcc cggggttggc aagagccggc tcgcccgcga gctgggcatc
      181 gagctcgaac ccgccggctg gcgggttctc tacggtcggt gcctgccgta tggcgagggg
      241 atcacgtact ggccgctggg cgaggtcatc cgcgacctgg ccgagatcac gcccgaaatg
      301 aacagcgccg tcgcccagca gcgcctggcc gcggtgtcac ccgaccgcga caccgccgaa
      361 cgcttggcat tcgcgatcgg cctccgcgcc gaggccccgg tcagcggcga gtcgctggac
      421 cgcgaaatcg cctatgccgt ccggcggctg gtggagtcgc cggcgacaga tagaccgatc
      481 ctgctggtgg tcgaggacat tcactgggcg gagccgccgc tcctggacct cctcgaatac
      541 ctcgccacct ggacccggga ccgatgcctc ctgatcgtcg ccctcgcccg acccgacctg
      601 atcgaccgcc gccccggatg gggctccggc cggatggagg cttcccgact ccagctcgag
      661 gccctgtccc gcgaagaagc aacctccctc gtccacgctt tgctcgcggc agacgggctg
      721 ccggaagacc tccgcgaccg gatcctcgac cgggccgagg gcaacccgtt gttcgtggag
      781 gagacgatcc ggatgctcat cgaccagggc gccgtggtcg aacgggacgg cgcctgggtc
      841 gcggccaacg agctggtcga ggtcgaggtg ccggagacga tcgaggccct cgtccgtgcc
      901 cgcatcgacg ccctgccgcg ggatgaccgt tcgatcctcc agtgtgcggc ggtgatcggc
      961 cgcacgttcc gccgatcggc cctcgccgcg ctggtggacg gaccggtcga cacgttcctc
     1021 gacgaggcaa tcctccgcga cctcgtcagc gaggagccgg cggcggaccc gtcgtaccgc
     1081 ttcaagcacc tcgtcatccg ggacgtggcc tatggctcac tacccaaggc ccggcgcgcg
     1141 gccctccacc gccgcaccgt ggagtggctg atcgaatggg ccgcggaccg tcgcgacgag
     1201 ttcgtggaga tcgaggccca gcacctcgag caggcggtga agctgttgaa cgaactggaa
     1261 ggccaggccg atggcgccct cgtcgaccag gccgtggccg cgctgcggcg gagcaccgag
     1321 aaggcgggcg ctcgcgacga cctgcgcgcg gtgatctcgt tcgcgaaccg ggctctcgcc
     1381 cttcacccgc gctcgacgga ggagcgactc gagctccaga ccatgctgct ggaggcgatg
     1441 ttcgaaagcg gggacttcgc ccatggccgc gacctggggg ccgcggtggc cgagcaggca
     1501 ggggccatcg gccgtcgtga ccttcgtggg cgggcgctcc tgcgcgcggg gc
//
LOCUS       DASYGU010000300         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_513766, whole genome shotgun sequence.
ACCESSION   DASYGU010000300 DASYGU010000000
VERSION     DASYGU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513766"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..1206
                     /locus_tag="VGO32_08570"
     CDS             469..1206
                     /locus_tag="VGO32_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200840.1"
                     /translation="MSQPLKLLGGAAALITAALVGGTLIGSVFAAPGGSGSADPAVAF
                     GDEETTDAAAYCQTFLDTFASELGVETDELAPAAKAAAIAAVNAAVEAGDLTQDAADR
                     MIEHINAWDGDGCRWIGFKLGHWGHHAAQVEFLTGMWEAAAGALDMTPAELREALADS
                     TLQEVAEAEGVAYADVVAAALASAQTDLDAAVEAGRITQDKADAILERLETWLNDGTI
                     GDWGDRRGWFNRGPLSPPADASEAPAT"
     gene            1362..2030
                     /locus_tag="VGO32_08575"
     CDS             1362..2030
                     /locus_tag="VGO32_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200841.1"
                     /translation="MAVSQTRRFSDTSPVREAGRSLRSQPRWVLRRFVARPLTGMFLV
                     VPLLGAVLGLGNPSILLTLSGAAAIYLALDLFLAWIGRPVSAEPASHLNLVAWTAGIA
                     VLAAAGWPEPAWQYHGELVTLVGMATGFAVGMGSPRAVAALWTVAAGAAVALGASLTG
                     PLTAEPAMVVAAIAIGTWFGAVVGLVVDRVAVPHRVRVPAPSAASSAPAGRAAAEVRA
                     NPPV"
     gene            complement(2027..2770)
                     /locus_tag="VGO32_08580"
     CDS             complement(2027..2770)
                     /locus_tag="VGO32_08580"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEV7200842.1"
                     /translation="MIDRAVSAYRVNSSAIAVALLIVTNLIPLGGVLWLGWSLLLILA
                     LYWAENGVVGVINVLKILTAKGTTTGPSAARWSVNGRPAASLSRLGTAGFFTIHYGLF
                     WIVHGIFVFTFIPAMTGIGGFGGFGPGFMFVTVPAGPPGLPGVDASVFAFGVIGLAIS
                     HGVSFWMNYLGRGEFRTLSPADVMIQPYGRLVIMHLTIILGAFVSIALGTSLGSLIVL
                     VVLKTALDLAFHLRQHREPAGSMGPVIAG"
     gene            complement(2788..>3117)
                     /locus_tag="VGO32_08585"
     CDS             complement(2788..>3117)
                     /locus_tag="VGO32_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7200843.1"
                     /translation="ELVLTLYAPEIEYWTEPFREPYRGLDGAREYATQSFASEHTVTA
                     WFGEPVVDGDRAAVPWWATQIEDDEPVTLAGISLLGFNADGLVVEEFDTWNSAEGMTP
                     HPPTWGR"
BASE COUNT          476 a         1072 c         1088 g          481 t
ORIGIN      
        1 gacccgctcc tcgtcgccct cattgaacgc cgcctcgacc gcggcgtcga tcgccgcttt
       61 gtcgacttcg gggggcatgt cagccacggc ccacaccgtg gtcggccatg gcacgtcggc
      121 cggctgagag gggtactggc cgagactcgg ggccggcgtg gagctgggag agctcggacc
      181 ggccgctggg ttgcaggcga ccacgagagc gaacgccgcg agccacatca gcctgggtcg
      241 tgtcctgcgg cgcgcaggca ggttcggcgg ccggcgtcga ttggtgttca tggcgcgctc
      301 cttcgtgcgg tatgggggcg accagcttcg ttttcttcaa accgccgccg aagcccgcgg
      361 ttcttgatgc gttcttcacc gcactcggcg acgctgcgcg gcactaaggg ggtcatccag
      421 cgtcctgtaa ccgctctccg gaaatccagc gaggtcaatg cacgagccat gtcgcagcca
      481 ctgaagctcc tgggcggcgc cgctgccctc atcaccgccg ccctggtggg tggaaccttg
      541 atcggatcgg tcttcgccgc gccgggcgga agcggatcgg ctgatccggc cgtcgccttc
      601 ggcgacgagg aaacgaccga tgccgccgcc tactgccaga ccttcctcga cacctttgcc
      661 tcggagctcg gggtcgagac cgatgagctc gcgccggccg ccaaggccgc tgccattgcc
      721 gcggtcaacg ccgcggtcga ggccggcgac ctgacccagg acgccgccga tcgaatgatc
      781 gagcacatca acgcctggga cggcgatgga tgccgatgga tcggcttcaa gctcggccac
      841 tggggccatc acgccgccca ggtcgagttc ctgaccggga tgtgggaagc cgccgcgggc
      901 gcgctggaca tgaccccggc tgagctgcgc gaggcgctcg ctgacagcac gctgcaggag
      961 gtcgccgagg ccgagggcgt cgcgtacgcc gatgtggtgg ccgcagcgtt ggcatcggct
     1021 cagaccgatc tcgacgcggc cgtcgaagcc ggcaggatca cccaggacaa ggccgatgca
     1081 atcctcgagc ggctcgagac ctggctgaac gacggcacga tcggtgactg gggcgaccgc
     1141 cgcggctggt tcaaccgcgg accactttcg ccaccggccg acgcgtccga ggcgccggcg
     1201 acctgactga gaggaacctc ggcgcgcgag gcgccgtcta gataggcgaa agggccgccc
     1261 aggctgtggg gcggcttttt cgttgccgtc gtcgtattcc ctcgccccct gggtaccttt
     1321 gggggatcgg cccggctggg gggcacacct agcatcggtc catggccgtc agccagaccc
     1381 gaaggttctc cgatacgtcg ccggtcaggg aagcgggtcg ctccctgcgc tcgcagcccc
     1441 gctgggtgct tcgccgcttc gtggcgcgcc cgctgaccgg gatgttcctg gtcgtccccc
     1501 tgctcggggc cgtcctgggt ctcggcaatc catcgatcct cctgaccctg agcggcgcgg
     1561 ctgccatcta cctggctctg gacctgttcc tggcctggat cggtcgcccg gtttccgcag
     1621 agcccgcttc ccacctcaac ctcgtggcgt ggacggcggg cattgcggtc ctggccgcgg
     1681 ccggctggcc cgagcccgcg tggcagtacc acggggagct tgtcacgttg gtgggcatgg
     1741 ccaccggctt cgcggtcggg atgggcagtc cgcgggcggt ggccgctctg tggaccgtgg
     1801 ccgcgggggc cgcggtcgcg ctgggtgcct cgctgaccgg gccgctcacc gccgagccag
     1861 cgatggtcgt ggccgcgatc gcgatcggga catggttcgg ggccgtggtc ggcctggtcg
     1921 tggaccgcgt cgccgtcccc caccgtgtcc gcgttccggc accatcagct gccagttccg
     1981 cgccagccgg acgggctgcc gccgaagttc gagcgaaccc gccggtctag cccgcgatca
     2041 ccggccccat gctgcccgcc ggctcacggt gctggcgcag atggaaggcg agatccagcg
     2101 ccgttttcag gaccacgagc acgatcagcg atcccagcga cgtgcccagc gcgatgctca
     2161 cgaacgcccc caggatgatg gtcaggtgca tgatcaccag ccgtccataa ggctggatca
     2221 tgacgtcggc cggggacagg gtccggaact cgccccgacc gaggtagttc atccagaacg
     2281 acacgccgtg gctgatcgcc aggccgatga cgccaaaggc gaagaccgat gcgtccacgc
     2341 ccggcagtcc gggcgggccg gccgggacgg tcacgaacat gaaccccggg ccaaagccgc
     2401 cgaacccgcc gattccggtc atggccggga tgaaggtgaa cacgaagatg ccgtgaacga
     2461 tccagaacag gccgtagtgg atggtgaaga accccgccgt gcccaggcgg ctgagcgagg
     2521 ccgcgggtcg gccgttgacc gaccagcgcg ccgcagacgg gccagtggtg gtgcctttgg
     2581 cagtgaggat cttcaggacg ttgatcacgc ccacgacgcc gttctcggcc caatacaggg
     2641 cgaggatgag gagaaggctc cagcccagcc acaggacgcc gcccagcggg atcaggttgg
     2701 tcacgatcag cagggccacc gcgatggcgg aggaattgac ccggtaggcc gataccgcgc
     2761 gatcgatcat ggccagccga tgttggttca gcggccccag gtcggcgggt gcggggtcat
     2821 gccctcggcg gaattccagg tgtcgaactc ctcaaccacc aggccgtccg cgttgaagcc
     2881 caggagcgag atcccggcca gggtcactgg ctcgtcgtcc tcgatctggg tcgcccacca
     2941 cgggaccgct gcgcggtcgc catccaccac cggctcgccg aaccaggcgg tcacggtgtg
     3001 ctcggaggcg aacgactggg tcgcgtactc gcgcgccccg tccagcccgc gatagggctc
     3061 acgaaatggc tcggtccagt attcgatctc gggggcatac agcgtgagca ccagctc
//
LOCUS       DASYGU010000301         5311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1131057, whole genome shotgun sequence.
ACCESSION   DASYGU010000301 DASYGU010000000
VERSION     DASYGU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131057"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VGO32_08590"
     CDS             complement(<1..1372)
                     /locus_tag="VGO32_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7200844.1"
                     /translation="MASATPSVAEVGADERQLARLGYRQQLSRSIGLLSNFSVGFTYL
                     SPVVGVYSLFAFGLATGGPAFIWTIPIVVFGQFMVVFTFSEIASEFPLAGGIYQWAKR
                     LVGPRYAFLSGWMYTAALLVTVAAVAYAANLFAAPLFGWEINDTTTILVAVTVIVLGG
                     ILNMLGIRRLALLARAGLVVEILGTIGLGTYLLLFDRNQDFGVIFDTMGAGGDNYLGA
                     FLAAALFSVWIFYGFEACGDIAEEVHDPSRKVPRAMQLTLAVGGATAAFLTLALILAV
                     PDIGAVMSGTDASPIDSILQANFGDTGATIALALILFAFVSCTIAIQGAAIRLVYSSA
                     RDGMIPGSRALSSVHPRLHMPPGAVLVAVIVPALITLLPAATVTIIITFAVTGIYIGF
                     QSVVLAALIARRRGWRPGGAFSMGSWGPMVNVIGLVYGVSAILILAYSTGDGLTEFVD
                     RWLVPIS"
     gene            complement(1629..2420)
                     /locus_tag="VGO32_08595"
     CDS             complement(1629..2420)
                     /locus_tag="VGO32_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV7200845.1"
                     /translation="MDYPTLRIAHLYPSLLNVAGDGGNVMALVQRCAWRGIPTEVVEV
                     EQGDTPDFTTFDLILFHGGQDVEMDVVARDLGLKVPSLQEAADQGVLIFAVCAGLQLL
                     GHRYIPSAGEPMEGAAILDLETHGGSQRFMQHAAVEVTLNGETGTVVGFENHSGQTIL
                     GPGAKPFGTIVAGAGNNGEDGTEGAVRDNVFATYLHGPVLPKNPWLTDQLIKIALERR
                     MGAPVELAPLDDTAEDRAHEVALAKAMRNRGEYTALTPAELVRNR"
     gene            complement(2420..3853)
                     /locus_tag="VGO32_08600"
     CDS             complement(2420..3853)
                     /locus_tag="VGO32_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEV7200846.1"
                     /translation="MTTAPFRLPPPRSAPPLTVRRFAAILAGKTAAVGSRLMGRGGGT
                     SIPGMVARRLDPGILKALVADQGIVVVAITGSNGKTTTARFAAAILRTAGLPVAHNQA
                     GANLAQGVTSLAISAADLRGHVSPVVMVTEIDEGALPRVAPEIRPRAVLVTNLFRDQL
                     DRYGEIYAVADAFEVVAAALPPDSVLVVNADDPIVAGLAPARDGRRVTFGMDLPASTD
                     TITRAADTIRCPACRANLEYEHVYLSHMGSWRCPACGLARPALDVAVTAVEVKGLAET
                     IFTVRTPGGEQEFSIPQSGLHVAYDAAAAVALAVGLGVPIDAAPAALAAVGPAFGRLE
                     RISAGQQDVVLGFAKNPTSYNATLRALATEELPRHLLIAVSNTLVDGEDFGWLWDVDF
                     ESAVPRIEAATVSGLRADELANRLKYAGLDPVRMTVIEERVPALDAALAATPAGEPLT
                     IVAGYTPTIELREEMRRRGWVGRYWEA"
     gene            complement(3854..>5311)
                     /locus_tag="VGO32_08605"
     CDS             complement(3854..>5311)
                     /locus_tag="VGO32_08605"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7200847.1"
                     /translation="TPILGAVQMIIGGEKVDAADGQTFEVINPATGELLATAPLGGPE
                     DVNRAVAAAQKALDEPKGFSSWSAAKRGRTLQKLSNLAKEHLEELAQLESQNVGKPIA
                     GARGEALAVGLVFEYYAGAANKHFGETIPTSAPGLDFTLREPIGVVGLIVPWNFPMNM
                     ASWKLGPALATGNACILKPASYTPLTAIKLGELALEAGFPPGIVNVVTGPGGSAGAAI
                     AAHPGIGKVAFTGETTTGQEILRLSSGNVKKVSLELGGKSPNVVFADADLDRFARESP
                     YAVFDNAGQDCCARSRIFVEASVHDQVVAAFTEATANLKVGNPADDKTEMGSLISLRQ
                     RERVAEYIQIGRDEGAELAYGGEAPTGDPFDDGAYLMPTIFDGCRTDMRIVREEIFGP
                     VVAIIPFSDEAEAIRLANDTPYGLSGSVWSRDIGKALRVAKGIRAGVLSVNSNSSVHT
                     EAPFGGYKMSGMGRELGMHALDLFTEVKNVFVDLR"
BASE COUNT          866 a         1794 c         1862 g          789 t
ORIGIN      
        1 cgctgatcgg cacgagccac cgatcaacga attccgtgag cccgtcgccc gtcgagtacg
       61 ccaggatgag gatggcggag acgccgtaca ccagcccgat gacgttgacc atcggccccc
      121 agcttcccat gctgaatgcg ccgcccggcc gccagccccg tcgccgagca atgagcgctg
      181 ccaggacgac gctctggaac ccgatgtaga tgcctgtaac agcgaaggtg atgatgatcg
      241 tcaccgtggc cgcgggcagg agggtgatca gggcggggac gatcactgcc accagtaccg
      301 cgccgggtgg catgtgcagg cgtggatgga cgctcgagag cgcgcgcgaa ccgggaatca
      361 tgccgtcccg cgcggagctg tagacgagac gaatggccgc gccctggatg gcaatcgtgc
      421 atgaaacgaa ggcgaagagg atcaacgcca gggcgatcgt cgctccagta tccccgaagt
      481 tcgcctggag gatcgagtcg atgggcgacg catcggtccc gctcatgacg gcaccgatat
      541 caggcaccgc caggattagc gcgagggtca ggaaggccgc ggtcgcaccg ccgacggcca
      601 gggtcagctg catggcgcgc ggtaccttcc gggacgggtc gtgcacttct tcagcgatgt
      661 caccgcaggc ttcgaagccg tagaagatcc agacgctgaa gagggcggcc gcgaggaagg
      721 cgccaagata gttgtcccca ccggcgccca tcgtgtcgaa aatgacaccg aagtcctggt
      781 tgcggtcgaa cagcagcagg tacgtcccca acccaatagt gcccaggatc tcgaccacca
      841 ggcccgctcg cgcaaggaga gcgaggcggc gaatgccgag catgttcagg atgccgccga
      901 ggacgatgac cgtcaccgcc accaggatgg tggtcgtgtc gttgatctcc cagccaaaga
      961 gcggcgctgc aaagaggttt gccgcatacg ccacggcagc cactgtgacg agcagcgccg
     1021 ccgtgtacat ccatccagac aagaacgcgt aacggggacc taccaggcgc ttcgcccatt
     1081 ggtagatgcc acccgccagc ggaaattcgg aggcgatctc ggagaaggtg aagaccacca
     1141 tgaactggcc aaagacgacg atggggatcg tccagatgaa cgccggaccg ccggtcgcca
     1201 gcccgaatgc gaacagggag tacaccccca ccaccggcga caggtaggtg aacccaaccg
     1261 agaagttcga gagcagcccg atggatcggc tcagttgctg ccgataccca aggcgcgcca
     1321 gctgacgttc gtcagcccca acctcggcga cggacggtgt cgcggatgcc atgaaaaccc
     1381 tcctccggat tcctggtccg cgcgcggggc gatgctcccg gcccggaaac ccgccaccgc
     1441 gaagcgcgta ggcgtcgaga gtgttaagta cgccaaccgc caggtcaaga ggaaattaag
     1501 gccggcgcga ccaggcgcgc gcgggcctgt cggagccggc ccagggcgcg accgacggtt
     1561 cgcgagaacg cgagcggccc gaccactcgc gcggcgggcc acttgcgtct tgggcgggcg
     1621 aatgaacgct agcggttgcg aaccagctcc gcgggcgtga gggcggtgta ctcgccgcgg
     1681 ttgcgcatgg ccttggccag ggccacctcg tgggctcgat cctcggccgt gtcgtcgagc
     1741 ggcgcgagct ccaccggcgc acccatccga cgctccagcg cgatcttgat cagctggtcg
     1801 gtgagccacg gattcttggg caggaccggg ccgtggaggt aggtcgcgaa cacgttgtcg
     1861 cgcaccgcac cctcggttcc gtcctcgccg ttgttgccgg ctccggccac gatcgtgccg
     1921 aacggcttcg cccctgggcc caggatcgtc tgccccgagt ggttctcgaa gccgacaacc
     1981 gtgcccgttt cgccgttcag ggtgacctcg accgccgcgt gctgcatgaa ccgctgcgag
     2041 ccgccgtggg tctcgagatc caggatcgct gcgccctcca tcggctcacc cgccgagggg
     2101 atgtaccgat gtccgagcag ctgcagcccg gcgcacaccg cgaagatgag gacgccctgg
     2161 tcggccgcct cctggaggga cgggaccttg agcccgaggt cgcgggccac gacgtccatc
     2221 tccacgtcct ggccgccgtg gaacaggatg aggtcaaagg tcgtgaagtc cggcgtgtcg
     2281 ccctgctcga cctcgaccac ctcggtcggg atgccgcgcc acgcgcagcg ctggacgagg
     2341 gccatgacgt tgccgccgtc cccggccacg ttcaggagcg aggggtacag gtgcgcgatc
     2401 cgaagggtgg ggtagtccat caggcttccc agtatcgccc aacccagccc cgccggcgca
     2461 tctcctcgcg gagctcgatg gtgggggtgt agccggccac gatggtcaac ggctcgccgg
     2521 ccggggtcgc ggccagggcg gcatccaggg caggcacccg ttcctcgatg actgtcatcc
     2581 gcaccgggtc caggccggcg tacttcagcc ggttggccag ctcgtcggca cgcaggcccg
     2641 agaccgtcgc cgcctcgatc cgcggcacgg cggactcgaa gtccacgtcc cacagccagc
     2701 cgaagtcctc gccgtcgacc agggtgttgc tgaccgcgat caggaggtgg cggggcagct
     2761 cctcggtcgc cagcgcgcgg agggtggcgt tgtaggaggt ggggttcttc gcgaatccga
     2821 ggaccacgtc ctgttggccg gcagaaatcc gctccagccg cccgaatgcc ggtcccacgg
     2881 cggccagtgc ggccggagcg gcatcgatcg gcacgcccag gccgacggcc agggcaacgg
     2941 cggccgcggc gtcgtacgcc acgtgcaggc ccgactgcgg gatcgagaac tcctgctcgc
     3001 cgccaggggt gcggaccgtg aatatcgtct cagccagacc cttgacctcg accgcggtga
     3061 ccgcgacgtc gagggccggg cgggcgaggc cgcaggcggg gcagcgccac gagcccatgt
     3121 gcgagaggta gacgtgctcg tactcgaggt tcgcgcggca ggcggggcag cggatcgtgt
     3181 cggcggcccg ggtgatcgtg tcagtcgagg cgggcaggtc catcccgaac gtcacccgac
     3241 gaccgtcccg ggccggggcc agtccagcca cgatcggatc gtcggcgttc acgaccagca
     3301 cgctgtcggg cggaagcgcc gcggccacca cctcgaacgc atcggccacc gcgtagatct
     3361 cgccgtagcg gtcgagctgg tcgcggaaca ggttggtcac gagcaccgcg cgcggtcgga
     3421 tctcgggcgc gacccggggc agggcgccct cgtcgatctc ggtgaccatg accaccggcg
     3481 agacgtggcc ccgtaggtcg gcggcggaga tggcgagcga ggtgacgccc tgcgccaggt
     3541 tcgccccggc ctggttgtgg gccaccggca ggccagcggt gcggaggatg gcggccgcga
     3601 accgggcggt ggtcgtcttt ccgttgctgc cggtgatggc aaccaccacg atgccctggt
     3661 ccgcgaccag ggccttgaga atgcccgggt ccaaccgtcg ggcgaccatg cccggaatcg
     3721 aggtcccgcc gccgcggccc atcagccgcg agccaaccgc ggcggtcttg ccggccagga
     3781 tggccgcgaa ccgtcgaacg gtcaggggcg gcgccgagcg gggcgggggc agcctgaacg
     3841 gtgcggtggt cacctatctc aggtcgacga agacgttctt gacctcggtg aacaggtcga
     3901 gcgcgtgcat gccgagctcg cggcccatgc cgctcatctt gtagcccccg aacggcgcct
     3961 cggtatggac gctgctgttc gaattcaccg agaggacgcc ggcgcggatg cccttcgcca
     4021 cgcgcagtgc cttgccgatg tcgcggctcc acaccgaccc cgacaggccg tacggcgtgt
     4081 cattggccag gcgaatggcc tccgcctcgt cgctgaaggg gatgatcgcg acgactgggc
     4141 cgaagatctc ctcgcgcacg atccgcatgt cggtccggca gccgtcgaag atggtgggca
     4201 tgaggtacgc gccgtcgtcg aacgggtcgc cggtcggcgc ctcgccgccg tatgccagct
     4261 ccgccccctc gtcgcggccg atctggatgt actccgctac ccgctcgcgc tggcgcaggc
     4321 tgatgaggct gcccatctcg gtcttgtcgt ccgccggatt gcccaccttg aggttggcgg
     4381 tggcttcggt gaaggcggcg acgacctggt catggaccga tgcctcgacg aagattcggc
     4441 tccgcgcgca gcagtcctgg ccggcgttgt cgaaaaccgc gtagggcgac tcgcgggcga
     4501 atcggtcgag gtcggcgtcc gcgaagacga cgttcgggct cttcccgccg agctcgaggc
     4561 tcaccttctt cacgttcccc gacgacaggc ggaggatctc ctggccggtc gtcgtctcgc
     4621 cggtgaaggc caccttgccg atgccggggt gggcggcgat cgcggccccc gcgctgccac
     4681 ccgggccggt caccacgttc acgatcccgg gcgggaaccc ggcctcgagg gccagctccc
     4741 cgagcttgat agcggtgagc ggggtgtagg aggcgggctt caggatgcag gcatttccgg
     4801 tggccagcgc cgggccgagc ttccaggagg ccatgttcat ggggaagttc cagggcacga
     4861 tcagcccgac caccccgatt ggctctcgaa gggtgaagtc cagtccgggc gcgctggtcg
     4921 ggatcgtctc gccgaagtgc ttgttcgcgg cgccggcgta gtactcgaac acgaggccca
     4981 ccgccagggc ctccccgcgc gcaccggcga tcggcttgcc cacgttctgg ctctcgagct
     5041 gggcgagctc ctcgaggtgc tccttggcca ggttgctcag cttctggagg gtccgcccgc
     5101 gcttggcggc cgaccaggag gagaagccct tcggctcgtc caatgccttc tgggcggcgg
     5161 caacggctcg attgacgtcc tccgggccac ccagcggggc ggtggccagc agctcgccgg
     5221 tggcaggatt gatgacctcg aacgtctggc cgtccgcggc gtcgaccttc tcgccgccga
     5281 tgatcatctg gaccgcgccg aggattggtg t
//
LOCUS       DASYGU010000302         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_307324, whole genome shotgun sequence.
ACCESSION   DASYGU010000302 DASYGU010000000
VERSION     DASYGU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307324"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VGO32_08610"
     CDS             <1..1378
                     /locus_tag="VGO32_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7200848.1"
                     /translation="EYTLQRAQKIVGMRTLLAIPLMSEGVPVGVLSVWRQKVDPFSDE
                     EVRLMATFAEQAAVTMRLARLLSETQEALDRESAVGGVLASIARSSFDLPSVLNAVTE
                     SAARLTHADSGNLAVIDGGEYRIAAAYGEKSAELKRIFEASRITLDRGSMAGRVTLER
                     RTVQIPDVLADPEYSQLQMQEVVGFRTLLGVPLMREGEPTGVLMMQRSEVRPFTDQEI
                     GLITTFADQAALAIANVQLYETVERQRTALASFAPHVAGLLSSPEGEALLTGHRREIS
                     ALFCDMRGFTAFAETAEPEELFSVLREYHAEVGRIALDNGGTVEHFAGDGFMIFFNDP
                     TPVADHPLAATRAALAMHDRFAELAEGWHRRGYDLGLGIGISVGYATLGRIGFEGRYD
                     YAGVGAVTNLAARLSSAAAAGETLLSQRAFAAVEGRVDAEPTEELSLKGFSHAVVAYR
                     VTGLRPDA"
     gene            complement(1375..>2928)
                     /locus_tag="VGO32_08615"
     CDS             complement(1375..>2928)
                     /locus_tag="VGO32_08615"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEV7200849.1"
                     /translation="PAGPNGTRLPTARSVTYDPTPAELQELTSRMSHARRTAFGNYNV
                     QTRVVSRSKASTYLVTDDPSITTGQTITRAEGARVAALQDAYIAEQEMVVVDGFIGND
                     PEYRSRARLYIETANANIAGMQEQLYFPPDRLSAEWVPELAMIYTPNLAMPGYPDDRL
                     IAVDLEAGVTRVFNSDYFGESKKGGLRMWNAKVFHAGGLAMHAGCKVVPTEAGERTIL
                     IIGLSGTGKTTTTFTRQNGSQPVQDDFVGLFPGGKVVGTENGCFAKTFALDPRHEPAI
                     HGAVTKPDAYLENVSQKEPDGPVDFFDEAYTKNGRATFPMASLGIWRDPRQMGPVNEL
                     LILNRNDNIIPGVARLSREQAAAFFMLGETKGTSAGGAAEEGKALRVPGTNPFYPYRD
                     EQQANRFWELMGTHPFDVYLVNTGRVGGADRDPNSKKLEIEHSGAIVKAIAEQSIAWE
                     SDPDFGYEVATAVPGIEDLELLQPRRLYERTGRADEYRTWVERLKRERAEFLAAYPGL
                     RSEILAAVS"
BASE COUNT          439 a          980 c         1017 g          492 t
ORIGIN      
        1 cgagtacacg ctgcagcgcg cgcagaagat cgtcgggatg aggaccctgc tcgcgattcc
       61 cctcatgagc gagggtgtgc ctgtcggcgt gctgtccgtc tggcggcaaa aggtcgaccc
      121 gttcagcgac gaagaggtcc gcctgatggc caccttcgcc gagcaggctg cggtcacgat
      181 gcgcctggcc cgtctgctca gcgagaccca ggaggcgctc gatcgcgagt ccgccgttgg
      241 gggggtcctc gcatcgatcg cccgcagcag cttcgacctg ccgagcgtgc tcaacgccgt
      301 gaccgagagc gccgcccggc tgacgcatgc ggacagcggc aacctcgcgg tcatcgacgg
      361 cggcgagtac cggattgcgg cggcctatgg cgagaagtcg gccgagctca agcggatctt
      421 cgaggcgtcg cggatcacgc tcgaccgggg ctccatggcg ggccgggtga cactcgagcg
      481 acggacggtc cagatccccg acgtcctggc cgatccggag tacagccagc ttcagatgca
      541 ggaggtggtg ggcttccgga cgctgctcgg cgtcccgctc atgcgcgaag gtgagccgac
      601 cggggtgctc atgatgcagc gatccgaggt ccgccccttc accgaccagg agatcggcct
      661 gatcaccact ttcgccgacc aggcggcgct ggccatcgcc aacgtccagc tgtacgagac
      721 cgtggagcgg cagcgcaccg ccctcgccag cttcgccccg catgtcgcgg gtctcctctc
      781 cagccccgag ggcgaggcgc tgctcaccgg acatcgccgc gagatcagcg ccctgttctg
      841 cgacatgcgc ggcttcaccg cgttcgccga gacggcggag cccgaggagc tgttcagcgt
      901 cctgcgcgag taccacgccg aggttggacg gatcgcgctc gacaacgggg gcacggtgga
      961 gcacttcgcg ggggacgggt tcatgatttt cttcaacgat ccgaccccgg ttgccgatca
     1021 tccactggcg gccacgcggg cggcgctggc catgcacgac cgattcgcgg agctggccga
     1081 agggtggcac cggcgtggct atgacctggg gctggggatc gggatctcgg tcggctacgc
     1141 aacgctggga cggatcggct tcgaaggccg atacgactac gccggggtcg gggcggtcac
     1201 caacctggcc gctcgcctca gctcagccgc cgcggccggc gagaccctgc tcagccagcg
     1261 ggcattcgcc gcggtcgagg gtcgggtgga cgccgaaccc accgaggagc tcagcctgaa
     1321 gggattcagc cacgccgtgg tcgcctatcg agtcaccggc ctgcggccgg acgcttagga
     1381 gacggcggcc aggatctccg aacggaggcc ggggtaggcg gccaggaact ccgcccgctc
     1441 ccgcttcaat ctctcgaccc acgtccggta ctcgtccgcg cgtccggtcc gctcgtacag
     1501 gcggcgcggc tggagcagct cgaggtcctc gatgccgggc accgcggtcg caacctcgta
     1561 cccgaagtcg gggtcagact cccaggcgat ggactgctcg gcgatggcct tcacgatggc
     1621 ccccgagtgc tcgatttcga gctttttcga gttcgggtcc ctgtcggccc cgccgacccg
     1681 tccggtgttc accaggtaca cgtcgaacgg atgggtgccc atcagctccc agaaccggtt
     1741 cgcctgctgc tcgtcgcgat acgggtagaa cggattcgta cccggtacgc gcagggcctt
     1801 gccctcctct gcggcaccgc cggctgaggt ccccttcgtc tcccccagca tgaagaacgc
     1861 ggctgcctgc tcgcgagaca gccgcgcgac gcccgggatg atgttgtcgt tgcggttgag
     1921 gatgaggagc tcgttgaccg gacccatctg gcgcggatcg cgccagatgc ccagcgaggc
     1981 catcggaaag gtcgcgcggc cgttcttggt ataggcctcg tcgaagaagt cgaccgggcc
     2041 gtccggttcc ttctgggaca cgttctcgag ataggcgtcg ggctttgtca ccgcgccgtg
     2101 gatcgccggc tcgtggcgcg ggtccagggc gaaggtcttc gcgaagcagc cgttctcggt
     2161 gcccaccacc ttgccgcctg ggaacaggcc gacgaaatcg tcctggaccg gctgtgaccc
     2221 gttctgacgg gtgaatgtcg tcgtcgtctt gccggtgccc gacaggccaa tgatcaggat
     2281 cgtgcgctca cccgcctcgg ttgggaccac cttgcagccg gcgtgcatcg ccagcccgcc
     2341 ggcatggaac accttggcgt tccacatccg caggccgccc ttcttcgact ccccgaagta
     2401 gtcggagttg aacacccggg tcacgccggc ttcgagatcc accgcgatga gccggtcatc
     2461 cgggtagccg ggcatggcca ggttgggggt gtagatcatc gccagctccg gcacccactc
     2521 ggccgacagc cggtcgggcg ggaagtagag ctgctcctgc atgcccgcga tattcgcgtt
     2581 ggcggtctcg atgtacagcc gggcccgaga gcggtactcg gggtcgttgc caatgaaccc
     2641 atcgaccacg accatctctt gctcggcgat gtacgcgtcc tggagggcgg caacccgtgc
     2701 cccttctgcg cgggtgatcg tctggccggt cgtgatcgag gggtcgtcgg tcaccaggta
     2761 ggtggatgcc ttcgagcgac tgacgacccg ggtctggacg ttgtagttgc cgaacgccgt
     2821 ccgtcgggcg tgggacatcc ggctcgtgag ctcctggagc tccgccgggg tggggtcgta
     2881 ggtgacggat cgagcggtgg ggaggcgggt cccattgggt cccgcggg
//
LOCUS       DASYGU010000303         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_411455, whole genome shotgun sequence.
ACCESSION   DASYGU010000303 DASYGU010000000
VERSION     DASYGU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411455"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VGO32_08620"
     CDS             <1..331
                     /locus_tag="VGO32_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HEV7200850.1"
                     /translation="TDPDGTVRLPFDPATGRLIPETWDRWLARDPVRMVSDHADALRG
                     LRAIWIDAGKSDEFFLDLGATAFRDELAAIGITEPTVHFELVEGSHSDLEHLYPVAIA
                     WLAERIA"
     gene            complement(349..2007)
                     /locus_tag="VGO32_08625"
     CDS             complement(349..2007)
                     /locus_tag="VGO32_08625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7200851.1"
                     /translation="MSQLVNRGVAELPVAGASDPRRVSRVRRASDRLTTGRLKRVRRL
                     ARDPYLVYTALLAPVAIAVASVSFFTDDTVSALTLSALFIGLQALAGLVPARHRFLTP
                     RGWSFLRLAIALLYVWALVNTVGGPARPLFPLYLPVVVAAAALGTAQVIVIGVTAAVI
                     YLVPELASLLDPAAVVNPLAVAELTPRGLALVGVSTVLAIGTRRLVVQLEDVSSQFRS
                     SAMSERRRSRQIAGLEAVSRLLVTAGATPELLERVADVMVQRFGYALAVIFIAEGDTL
                     KLGAQRGYAIDMTHFDPLTGVVGRAARTGEVQLVQNVESDPDYVPGDQRVVSEIAAPM
                     VLDGVLLGVLNIESIAREPLDRIDRDLVATMAGKIAAALALSRDRQALADLAVRDGLT
                     GLHNRRFFDDSVSRLLTARAAMPESERRPVAAILFDLDHFGTFNKLHGVQIGDQALRV
                     FAHVLRERVRQTDLIARYGGEEFVVVMDGADRDQAVHVAEEVRLRLGSRTIPAEDGAP
                     LRLTVSAGCAALDDLEPSAEALLRTADVALSMAKRGGRDRVVAA"
     gene            2087..3106
                     /locus_tag="VGO32_08630"
     CDS             2087..3106
                     /locus_tag="VGO32_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7200852.1"
                     /translation="MRIAHIRERNAPIGTPWRLAAARDAAGDRWLDLEAARVALVADD
                     PRRAHNEPLFRQPVTTLDAHLGRGLRVAALGALVDGVAGNDPDDAVWAAADLLFGPPI
                     LQPPSFRDFYAFEQHVGTMWQRRGHDIPEAWFRLPIFYFSNTSEIRGPDEAVWAPAGS
                     VELDYELEVGALVDTPAVDLAADRGEEAIGGFLILNDWSARDLQREETTVRLGPAKGK
                     DFATTIGPWLVTPDELADARTDSGFELAMTAEVNGTEVSRGTWSAAHFSFGDMVARAS
                     ADVRLRPGDLLGSGTVGTGCLLEVREERFGRWLEPGDQVTLRIERMGELRTPVVSRPE
                     GRNAL"
     gene            3103..3597
                     /locus_tag="VGO32_08635"
     CDS             3103..3597
                     /locus_tag="VGO32_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7200853.1"
                     /translation="MNEHMEVLARLAVAEGRLAALAEDPPPGLTDPDPATGEQWDAGQ
                     AWAHVAEFVPYWQGEMEDVVTGAARGEEPVPFGRTTADPGRVGAIEQGRNEPPAAQMT
                     RLARALGHMRSYLLRLSAPEWSARGLHARLGEMTVAQIVDRFVVGHLEEHADQLEKLK
                     TDPR"
     gene            complement(3594..4736)
                     /locus_tag="VGO32_08640"
     CDS             complement(3594..4736)
                     /locus_tag="VGO32_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200854.1"
                     /translation="MTSATTPSDRAAHLVGSTPFADTAEAIDIFADKLGPFLRNVPDG
                     ETGSRKNWIQILIQSFFDNPDLESKKGGPDDFDYSDYDRTPSFKVRSGHRLTGDSFDF
                     GYLSAFRESWPEFAKRRDAFHPPGNGVQPRFQVGIPGDLDLAVSSFGSPIAGIRNRDA
                     FRDATVRDIRAIHAEAGDDVVFQVEIPVELVALTRIPGFLHGPVTGYLSGGVARLARE
                     APEGSRWGIHLCLGDFNHRAMGRLPDVGPIVRMSNEIVKRWPAGRPLDFIHAPLAAAA
                     EPPPLEPAFYEPLRGLNLPAGTRFVAGFVHESRTLEEHRTLMAQIDDLVGHPADIACS
                     CGLGRRSREEALAIIDTTAELLGGGPRREAFPIADATPADATSAAV"
BASE COUNT          754 a         1649 c         1769 g          680 t
ORIGIN      
        1 caccgatccc gacggtacgg tccgactccc gttcgacccg gccaccggcc gcctcatccc
       61 cgagacctgg gaccgatggc tcgcccggga cccggtgcgg atggtcagcg accacgcgga
      121 cgcgctccgt ggtctccgcg cgatctggat cgatgccggg aagagcgacg agttcttcct
      181 cgacctgggc gcgaccgcct tccgggacga gctcgcggcc atcgggatca ccgagccgac
      241 cgtccatttc gagctggtgg aagggtcgca ttccgacctc gagcacctgt atccggtggc
      301 catcgcctgg ctggccgaac ggatcgcctg accgccccag gaacgcagct aggcggccac
      361 cacccggtcg cgaccgccgc gcttggccat agacagggcc acgtccgcgg ttcggaggag
      421 ggcttcggcc gacggttcca ggtcgtccaa ggccgcgcac ccggccgaga cggtcagacg
      481 gagcggagcg ccatcttcgg ccgggatcgt ccgtgacccg agcctgagcc tgacctcctc
      541 ggccacgtgg acggcctgat cgcggtcggc gccgtccatg accaccacga actcctcgcc
      601 gccgtagcgc gcgatgagat cggtctggcg tacccgttcg cgcaggacgt gggcgaagac
      661 ccggagcgcc tggtcgccga tctggacgcc gtggagcttg ttgaacgtcc cgaagtggtc
      721 gaggtcgaac aggatcgccg ccactgggcg gcgctcggac tcgggcatcg ccgcccgtgc
      781 ggtcaggagg cgggacacgc tgtcgtcgaa gaaccgccgg ttgtggagcc cggtcaggcc
      841 gtcgcggacc gcgagatcgg ccagggcctg gcggtcgcgc gacagggcca gcgcggcggc
      901 gatcttgccg gccatggtcg cgaccaggtc acggtcgatg cggtcgagcg gctcccgcgc
      961 gatggactcg atgttcagca ccccgagcag cacgccgtcc aggaccatgg gggcggcgat
     1021 ctcgctcacc acccgctgat cgccgggcac gtagtccggg tcgctctcga cgttctgcac
     1081 gagctggacc tcgcccgtcc gggccgcacg gccgacaacg ccggtcagcg ggtcgaaatg
     1141 ggtcatgtca atcgcataac cgcgctgggc gccgagcttc agggtgtcgc cctcggcgat
     1201 gaagatgacc gccaacgcgt atccgaagcg ctggaccatg acgtcggcca cccgctcgag
     1261 gagctcgggt gtggcgccgg ccgtgacgag cagccggctg accgcctcga gacccgcgat
     1321 ctggcgcgat cgacggcgtt cggacatggc gctcgagcgg aactggctgc tcacatcctc
     1381 gagctggacg acgaggcgcc gggtcccgat ggcgagcacg gtggagacac cgaccagggc
     1441 gaggccccgg ggcgtgagct cggccacggc cagcgggttg acgacggctg ccgggtcgag
     1501 gaggctcgcc agctccggga cgaggtagat gaccgcggcg gtgacgccga tgacgatgac
     1561 ctgtgccgtg cccagcgcgg cggccgcgac cacgaccggc aggtagagcg ggaacagcgg
     1621 acgggccggt ccgccgaccg tgttgaccag cgcccaaaca tagaggagcg cgatcgcgag
     1681 gcgaaggaac gaccacccac ggggggtcag gaaccgatgg cgggccggca ccaacccggc
     1741 gagcgcctgg aggccgatga acagcgcgct gagcgtcagg gcgctcacgg tgtcgtcggt
     1801 gaagaagctg accgatgcga ccgcgatcgc gaccggggcg agcagcgcgg tgtagaccag
     1861 gtacgggtcc cgcgcgaggc ggcggacccg tttcaggcgg ccggtggtca ggcgatcact
     1921 cgcgcgccgg acgcgcgaaa cccgccgggg atcggacgca ccggccaccg gcagctcggc
     1981 aaccccccgg ttgaccaact gcgacatcgg tgcaaaaccg tacccaacca ctcagcagcg
     2041 caccatgagc cggaagtacc gatatcgccg cgtacactcc tccgcgatgc gcatcgccca
     2101 cattcgcgag cgaaacgctc cgatcggtac gccctggcgg ctggcggcgg cccgcgacgc
     2161 ggccggcgat cgatggctgg acctcgaggc ggcccgcgtg gcgctggtcg cggacgaccc
     2221 gaggcgcgcc cacaacgagc cgctgtttcg tcagccggtc accacgctgg acgcccatct
     2281 gggccgcgga ctccgggtcg ccgcgctcgg tgcgctggtc gatggcgttg ccggcaacga
     2341 tcctgacgac gcggtctggg cggccgccga cctgctcttc ggcccgccga ttcttcagcc
     2401 gccctcgttc cgcgatttct acgccttcga gcagcacgtt gggaccatgt ggcagcggcg
     2461 cgggcacgac atccccgagg cctggttcag gcttccgatc ttctacttca gcaacacctc
     2521 ggagattcgc gggccggatg aggcggtgtg ggcgccggcg gggtcggtcg agctggacta
     2581 cgagctggaa gttggcgccc tcgtcgacac gccggcagtg gatctcgccg cggatcgggg
     2641 ggaggaggcc atcggcgggt tcctcatcct gaacgactgg tcggcgcgcg acctccagcg
     2701 cgaggagacg accgttcgcc tgggcccggc caagggcaag gacttcgcca ccaccatcgg
     2761 tccgtggctc gtcaccccgg acgagctggc cgatgctcgc accgactccg ggttcgagct
     2821 ggcgatgacc gccgaggtca acgggaccga ggtcagccgc gggacgtggt ccgccgcaca
     2881 cttctcgttc ggcgacatgg tggcccgtgc gtcggccgat gtccgccttc gccctggcga
     2941 cctgctcggc agcggcacgg tgggaaccgg ctgcctgctc gaggtccgcg aggagcgatt
     3001 cggccgctgg ctcgagcccg gcgaccaggt gacactccgg atcgagcgga tgggtgagct
     3061 gcgaacgccg gtcgtgagcc ggcccgaagg gaggaatgcg ttgtgaacga gcacatggag
     3121 gtcctcgccc gactcgcggt agccgagggt cggctggcgg cgctcgccga ggatccgccg
     3181 cccgggctga ccgatcccga cccggccacc ggcgagcaat gggatgccgg ccaggcgtgg
     3241 gcccacgtgg ctgagttcgt cccctactgg cagggtgaga tggaggacgt ggtgaccggc
     3301 gcggcgcgcg gcgaggagcc ggtgcccttc ggtcggacca ccgccgatcc gggtcgggtg
     3361 ggtgccatcg agcaggggcg aaacgagccg cccgcagcgc agatgacccg cctggcgcgg
     3421 gccctcggcc acatgcggag ctacttgctg aggctcagcg caccggagtg gtcagcccga
     3481 ggcctccacg cccggctggg cgagatgacg gttgcccaga tcgtggaccg gttcgtggtc
     3541 ggtcatctcg aggagcatgc cgatcagctc gagaagctca agaccgatcc gcgctagacc
     3601 gcggctgagg tcgcgtcggc cggagtggcg tcggcgatgg ggaaggcctc ccgacgagga
     3661 ccgccaccga gcagctccgc ggtcgtgtcg ataatcgcca acgcctcctc ccgcgaccgg
     3721 cggcccaggc cgcaggagca cgcgatgtct gccgggtggc cgaccaggtc gtcgatctga
     3781 gccatcagtg tccggtgctc ctcaagggtt cgactctcat gcacgaaacc ggcgacaaac
     3841 cgggtgccgg ccggcaggtt gaggcccctg agcggctcgt agaacgccgg ctcgaggggc
     3901 ggcggctcgg ccgcggcggc cagcggagca tggatgaagt cgagtggacg accggcgggc
     3961 caccgcttca cgatctcgtt ggacatgcgc acgatcggcc caacatccgg cagccggccc
     4021 atggcccgat ggttgaaatc gccgaggcaa aggtggatcc cccaccgcga gccctcgggc
     4081 gcttcccgcg cgagccgcgc tacaccgccg gacaaatagc ccgtgaccgg cccgtgcagg
     4141 aatcctggga tccgggtcag cgccaccagc tcgaccggga tctcgacctg gaacacgacg
     4201 tcatcccccg cctcggcgtg aatggctcgg atgtcgcgga cggtcgcgtc gcggaaggcg
     4261 tcgcggttgc gaatgcccgc gatcgggctc ccgaacgagg agacggcgag gtccaggtca
     4321 cccgggatgc cgacctggaa ccgcggctgc accccgttgc ccggcgggtg gaacgcgtcg
     4381 cgccgcttgg cgaactcggg ccacgactcg cggaaggcgc tcaggtagcc gaagtcgaac
     4441 gagtcgccgg tgagccgatg gccggagcgc accttgaagc tcggggtccg gtcgtagtcg
     4501 ctgtagtcga agtcgtcggg cccgcccttc ttcgactcga gatcggggtt gtcgaagaac
     4561 gactggatga ggatctggat ccagttcttg cggctgccgg tttcgccgtc cgggacattg
     4621 cgcaggaacg ggccgagctt gtcggcaaag atgtcgatcg cctcggcggt atcggcgaag
     4681 ggggtgctgc cgaccaggtg ggcggctcgg tcggatggag tggtggcgga cgtcatgcct
     4741 cggcctccta tcggtcctcg cgggggcacc gctttcgcct tgagggaggc gggtcggttg
     4801 ccgggccgtc aacgagcgag gtctctcggg ccactctgga taggtgtcac gc
//
LOCUS       DASYGU010000304         5789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1182868, whole genome shotgun sequence.
ACCESSION   DASYGU010000304 DASYGU010000000
VERSION     DASYGU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182868"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1488
                     /locus_tag="VGO32_08645"
     CDS             226..1488
                     /locus_tag="VGO32_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200855.1"
                     /translation="MSPRLRRIVAVVTLLLALVLVAVLTLRALGVPIALGPETATPMP
                     TPGASAEPSTEATPSLDEALAAIEADTADIRDLPAADIGPPEFVTRDELERRLAEQFA
                     EDYPAEQQAVDNALYHALGILAPDQDYAALQLQLLSSQVIGYYDDAIQSMVIVGDAGL
                     TPEAEVTYSHEYTHALQDAAFGMDSLDLDNVDDNDASYAALALVEGDATTTMVLWAIQ
                     NLTPEEILDISQTPVPDTSGVPAWMVSQLEFPYLAGSEFTSQLYASGGFSAVDEAWAD
                     PPVSTEQVLHFRAYLDNEEPVEVIGSPPAGAHVEQVLESTFGEAMISIWLAALGVDQV
                     DADAAAEGWGGDAISVLTSADGADVGVELTIAFDTPADADQFEAAYADATGRLTGYSS
                     LFRSRETEVIVQQGTTEQLFIGTGQLTN"
     gene            complement(1507..3132)
                     /gene="argS"
                     /locus_tag="VGO32_08650"
     CDS             complement(1507..3132)
                     /gene="argS"
                     /locus_tag="VGO32_08650"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV7200856.1"
                     /translation="MTVRAELQAAIVAARDRAVAAGELALPPDQALPEPTVQRPAHAE
                     HGDYATNAAMQLAPLARSAPMAIADVLVRHLELPASVGETTVLPPGFINWRLDPAWVA
                     GQVPAILAAGANWGRTDGMAGQRFNVEYISANPTGPLHIGNGRGGFIGDALANLLEAT
                     GADVTREYYVNDYGSQVRDFGQSVYLARTGQSGEAGYRGAYIGELAAAVPDEVVASLN
                     PLEAIGEWAWNRAYDDIKKTIARLRMRIDVYRSERELHESGEVTAAIEQLRTAGHVYE
                     DEGATWFRSTTFGDDKDRVLIRSNGQPTYFAADVAYLLDKFERGANRLIYVLGPDHHG
                     TIKRWKGAAAGLGHDPDAVEFIIYQHVSLAGGAAMSKREGSYVTLDELVDEVGADATR
                     FLFTLRSANQHLEFDMDLARQQTNENPVYYVQYAHARCSSILRQPAADALDADPGDAT
                     VLLVHPAEQTLIRHLMRLPEAVADSADRRETHELPRYAMEVANLFSQFYRDCRVLTDD
                     VALSTARVGLVRTTRQVLANSLALIGVSAPDTM"
     gene            complement(3129..4430)
                     /locus_tag="VGO32_08655"
     CDS             complement(3129..4430)
                     /locus_tag="VGO32_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200857.1"
                     /translation="MTLRRALPAALLIGVAGWLLVWFQTEPRANFDSLIYHTHALEYA
                     GLDREEADAVSWQIFARYATERERTVVAETIGETWSTPADGRWMDLYQMRPLYPLLVA
                     AAYPVVGERAPMTVSAAVTIGFVMTTALGFGLLFGWRAAGFASLAALLQVHFTQWLVF
                     LATDGLAMTLWAATLLAVAFHARTGRWAWLAVIGLAVLALGLTRPTSSLAPLVPAVCA
                     GAAFVAREPVWRRFGVATAAALVPAAAVVLAQGLLGFPGVVDVLQEMPTRHFALPDIE
                     DPVGYTIRVLQWAVPQRLLPTLVEQPLLLATVAAGIAGLALNRSWTAAVFLSGLVVVP
                     VAWVIHPVWFDGGRILAPAWVSLSLGVGLFAEWLLVATRQPIGQAVDWATRPTTAPPV
                     EIPVARPETQPIPVRAPPATGVAWHAATARHRLGKAPEVEA"
     gene            complement(4427..5341)
                     /gene="ftcD"
                     /locus_tag="VGO32_08660"
     CDS             complement(4427..5341)
                     /gene="ftcD"
                     /locus_tag="VGO32_08660"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEV7200858.1"
                     /translation="MASLIECVPNFSEGRRQEVIDEITRAIRQIDGVSLLDVSRDESH
                     NRSVVTFAGRPEPVARAAMAAVGRALELIDMEQQTGAHPRIGAVDVIPFVPLGETRMD
                     ECVDLARRFGEQVAKVFDLPVYLYGEAARRPERRRLADIRRGQYETLREEIGTNPDRA
                     PDYGPARLHPRGGAVAVGARKPLIAFNLNLATDDVEIAQHIAETIRESSGGLPAVQAM
                     GVLVAVRDGRQLAQVSMNLADWERTGIPTVVAEVRRLARHAGTDIDHAELIGLAPTAA
                     LLEVVADALPLPGLSPDQALELRLARDQ"
     gene            complement(5329..>5789)
                     /locus_tag="VGO32_08665"
     CDS             complement(5329..>5789)
                     /locus_tag="VGO32_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200859.1"
                     /translation="GADWAAARELALKVEEGVHLPTAGHELETVRHGHWAAANERTGL
                     VLVLADGEDRGTDLTERAATVLRSASILGMPTVTIAATDRAAAAGPTPVVPVALDRQL
                     DRLTAAILGVVTPLQLLTERLARARGTNPDTIGRDDPRQAAASDFGPWQA"
BASE COUNT          892 a         1999 c         2079 g          819 t
ORIGIN      
        1 ccataccagg tgatctccac gggtccggga tgctaccgca gaacggtccc cggaaaagca
       61 cgagcgccgg tgcccgccca atgacaccgg cgcccgaagg agggagggtg gaggagaaat
      121 cccgaaggat ttctgactcg cacgatcaca gtaggttcgc tccgtaaagg gacaaccccc
      181 gcatttgtga agaaacctga acctttgccc cacaatgcct gaccgatgag cccacgcctt
      241 cgacggatcg tcgccgtcgt caccctgctc ctcgccctcg tcctggtggc ggtgctgacc
      301 ctgcgcgccc tcggcgtccc gatcgcgctc gggccggaga cggcgacgcc catgccgacg
      361 ccaggcgcgt cggcggagcc gagcaccgag gcgacaccgt cgctcgacga ggcactggcc
      421 gccatcgagg ccgataccgc agacatccgt gacctgccgg cggcggacat cgggccaccg
      481 gagttcgtga cccgtgacga gctcgagcgc cgcctggccg agcagttcgc ggaggactac
      541 cccgcggagc agcaggcagt ggacaacgcg ctgtaccacg ccctggggat ccttgccccc
      601 gaccaggact acgccgccct ccagctccag ctcctgtcca gccaggtcat cggctactac
      661 gacgacgcca tccagtccat ggtcatcgta ggcgacgcgg gcctgacgcc ggaggcggaa
      721 gtcacgtact cccacgagta cacccacgcc ctccaggacg cggcattcgg gatggactcc
      781 ctggacctgg acaacgtcga cgacaacgac gcgtcatacg cggccctcgc cctggtcgag
      841 ggtgatgcga ccaccaccat ggtcctgtgg gccatccaga acctcacccc cgaagagatc
      901 ctggacatca gccagacgcc ggtccccgat acgagcggcg tgcccgcctg gatggtcagc
      961 cagctcgagt ttccgtatct cgccggctcc gagttcacct cgcagctgta cgcgagcggc
     1021 ggattcagcg cggtggacga ggcgtgggct gatccgccgg tctcgaccga gcaggtcctc
     1081 cacttccggg cctatctcga caacgaggag cccgtcgagg tcatcggcag ccccccggcc
     1141 ggcgcccacg tggagcaggt cctggagtcc accttcggcg aggcgatgat cagcatctgg
     1201 ctcgcggccc tgggcgtgga ccaggtggac gccgatgccg cggcggaggg ctggggcggc
     1261 gacgcgatct cagtcctcac ctcggccgac ggtgcggacg tgggggtcga gctgaccatc
     1321 gccttcgaca ctccggccga cgccgaccag ttcgaggcgg cctatgccga tgcgaccggc
     1381 cggctgaccg gatacagcag cctcttccgg tcgcgcgaga ccgaggtgat cgtccagcag
     1441 ggcaccacgg agcagctgtt catcggcacc ggccagctca cgaactagga gccacgaccg
     1501 ccgcgtctac atcgtgtccg gagcggagac cccgatcagg gccaggctgt tggccagcac
     1561 ctggcgggtg gtccggacca acccgacccg cgcggtgctg agcgccacgt catcggtcaa
     1621 gacccggcag tcgcggtaga actggctgaa caggttcgcg acctccatcg cgtagcgcgg
     1681 cagctcgtgg gtctcgcgcc gatcggcgga gtcggcgacg gcctccggca ggcgcatgag
     1741 gtgccggatg agcgtctgct cggcggggtg gacgaggagc acggttgcgt cgcccggatc
     1801 ggcgtccagg gcgtccgccg ccggctggcg gaggatcgac gagcagcggg cgtgggcgta
     1861 ttggacgtag tagaccgggt tctcgttcgt ctgctgacgg gcgaggtcca tgtcgaactc
     1921 gaggtgctgg ttcgccgagc ggagggtgaa caggaagcgg gtggcatcgg cccccacctc
     1981 gtccaccagc tcgtcgaggg tcacgtagct cccctcgcgc ttgctcatcg ccgcgccacc
     2041 ggccagggac acgtgctggt agatgatgaa ctcgaccgcg tccggatcgt ggccgagccc
     2101 tgcggcggcg cccttccagc gcttgatcgt cccgtggtgg tccgggccga ggacgtagat
     2161 caggcgattc gccccccgct cgaacttgtc gagcaggtac gcgacgtcgg ccgcgaagta
     2221 cgttggctgg ccgttggaac ggatcaggac gcggtctttg tcatcgccga aggtcgtcga
     2281 ccggaaccat gtggccccct cgtcctcgta cacgtggccg gcggtgcgga gctgctcgat
     2341 ggccgcggtc acctcccccg actcgtggag ctcgcgctcg gaccggtaca cgtcgatccg
     2401 catccggagc cgggcgatcg tcttcttgat gtcgtcgtac gcccggttcc aggcccactc
     2461 gccgatcgcc tccagcgggt tcaacgaggc gacgacctcg tccggcaccg cggccgccag
     2521 ctcgccgatg tacgcgccgc gatagccggc ctcgcccgac tgcccggtcc gcgcgagata
     2581 gaccgattgg ccgaagtcgc ggacctggct gccgtagtcg ttcacgtagt actcgcgcgt
     2641 cacatccgct ccggtggcct cgaggaggtt ggccagcgcg tcgccgatga atccaccccg
     2701 cccattgccg atgtggagcg gccccgttgg gttggcgctg atgtactcga cgttgaaccg
     2761 ctggccggcc atgccgtcgg tgcgccccca gttcgcgccg gcggccagga tcgcaggcac
     2821 ctggcccgcc acccacgccg ggtcgagccg ccagttgatg aagccgggcg gcaggacggt
     2881 ggtctcgccc acgctggcgg gtagctcgag atggcggacg aggacgtccg cgatggccat
     2941 tggcgcggaa cgggcgagcg gtgccagctg catcgccgcg ttggtcgcgt agtcgccgtg
     3001 ctcggcgtgg gcgggacgct ggacggtggg ctcggggagc gcctggtcgg ggggcagggc
     3061 cagctcgccg gcggccacgg cccggtcgcg cgcggccacg atcgcggcct ggagctcggc
     3121 gcgcacggtc acgcctccac ctcgggagcc ttccccagcc ggtggcgggc tgtcgccgca
     3181 tgccacgcga cgccggtcgc aggcggtgcg cggaccggga tcggctgggt ctccggccgc
     3241 gcgacaggga tctcgaccgg cggagcggtc gtcggtcggg tcgcccagtc gacggcctga
     3301 ccgatcggtt gtcgcgtcgc gacgagcagc cactcggcga acagcccgac gccgaggctc
     3361 aggctgaccc aggccggcgc aaggatccgg ccgccgtcga accacaccgg gtggatgacc
     3421 caggccaccg ggacgacgac gagcccactg aggaagacgg cggcggtcca cgaccggttg
     3481 agcgcgagcc cggcgatgcc ggcggccacg gttgccagga gaagcggctg ctcgacgagc
     3541 gtgggcagga ggcgctgggg aacggcccac tggagcacgc ggatggtgta cccgaccgga
     3601 tcctcgatat cgggcagcgc gaaatgccgg gtgggcattt cctgcaggac gtccaccacg
     3661 ccgggaaagc cgagcaggcc ctgggcaagc acgacggccg ccgccgggac cagcgcggcc
     3721 gcggtcgcga cgccgaaccg ccgccacacc ggctcgcggg caacgaaggc ggcaccggcg
     3781 catacggccg ggaccagcgg cgcgagcgag ctcgttggcc tggtgagtcc gagcgccaac
     3841 accgccagcc cgatgacggc gagccacgcc caccgcccgg tccgagcgtg gaaggccacc
     3901 gccaggagcg tcgccgccca caaggtcatc gccagcccgt cggttgcgag gaacacgagc
     3961 cactgcgtga aatggacctg gaggagcgcg gcgaggctgg cgaacccggc ggctcgccag
     4021 ccgaacagaa gcccgaaccc gagcgcggtc gtcatgacga agccgatggt caccgccgca
     4081 ctcaccgtca tcggtgctcg ctcgccgacc accggatagg ctgcggcgac gaggagcggg
     4141 tagagcgggc gcatctggta gaggtccatc cagcggccgt cggcgggggt gctccacgtc
     4201 tcaccgatcg tctcggccac gacggtacgc tcgcgctcgg tggcgtagcg ggcaaagatc
     4261 tgccagctga ccgcatcggc ctcctcgcgg tcgaggcccg cgtactcgag ggcgtgggtg
     4321 tggtagatca gcgagtcgaa gttcgcccgg ggctcggtct ggaaccagac gaggagccag
     4381 ccggcgacac caatgagcag agcggcaggc agggcacgac gcagcgtcat tggtcccgtg
     4441 ccagccggag ctcgagcgcc tggtcgggag aaaggccggg gaggggcagg gcgtcggcga
     4501 cgacttcgag cagggcagcc gtgggggcaa gaccgatgag ctcggcgtgg tcgatgtcgg
     4561 ttccggcgtg gcgggcgaga cggcggacct ccgccacgac ggtcgggatc ccggtccgct
     4621 cccagtcggc gaggttcatg ctgacctggg ccagctggcg tccatctcgg acggcgacga
     4681 gcacgcccat ggcctggacg gcagggaggc caccagacga ctcccggatc gtttcggcga
     4741 tgtgctgggc gatctcgacg tcgtcggtgg cgaggttcag gttgaaagcg atgaggggct
     4801 tgcgggcgcc gaccgcgacc gcgccgccgc gcggatggag gcgggctggg ccgtagtccg
     4861 gagcgcggtc cgggttcgtc ccgatctcct cgcggagggt ctcgtactgc ccccgtcgga
     4921 tgtcggccag acgccgacgc tctggtcgtc gcgccgcttc gccgtacagg tagaccggga
     4981 gatcgaagac cttggcgacc tgctccccga aacgccgggc gaggtcgacg cactcgtcca
     5041 tccgcgtttc gccgaggggc acgaagggaa tgacgtccac ggcgccgatc cgcggatggg
     5101 ctcccgtctg ctgctccatg tcgatcagct cgagggcccg cccgaccgcc gccatggccg
     5161 cccgggcgac cggctccggt cgcccggcga aggtcacgac gctccggttg tgggactcgt
     5221 cccggctgac gtcgagcagg ctgacaccgt caatctgccg gatcgcccgg gtgatctcgt
     5281 cgatgacctc ttgacggcga ccttcgctga agttcgggac gcactcgatc aggcttgcca
     5341 tggaccgaaa tccgatgccg ccgcctgacg tggatcgtcc cgtccaatgg tgtccgggtt
     5401 cgtcccgcgc gcccgcgcga ggcgctcggt cagcagctgg agcggagtca cgaccccaag
     5461 gatggcagca gtgagccggt cgagttgtcg atcgagcgca acaggaacca cgggtgtcgg
     5521 gccggctgcg gcggcacggt cggtcgcggc gatcgtgacc gtgggcatgc ccaggatcga
     5581 ggcggatctc aggacggtgg ccgctcgctc cgtgaggtcg gtgccgcggt cctccccgtc
     5641 ggcgaggacg agaacgaggc cggtgcgctc gttggctgcg gcccagtggc cgtggcggac
     5701 cgtctcgagc tcatgtccgg ccgtcggcag gtgcacgccc tcctcgacct tcagggccag
     5761 ttcccgggcg gccgcccagt ctgcgccgg
//
LOCUS       DASYGU010000305         5386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_822778, whole genome shotgun sequence.
ACCESSION   DASYGU010000305 DASYGU010000000
VERSION     DASYGU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822778"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1414
                     /gene="accC"
                     /locus_tag="VGO32_08670"
     CDS             32..1414
                     /gene="accC"
                     /locus_tag="VGO32_08670"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV7200860.1"
                     /translation="MVSKILIANRGEIAVRILRTCREMGIPAVVAFSEPDRESMAVRQ
                     ADEAICIGPAEARRSYLNQPALISAALITGCDAIHPGYGFLSEDASFAEACAVHDLAF
                     IGPRAQVIERFASKYAVRRMLAATGLPTVPGSTGIVADLADALAQAEQAGYPVLLKPS
                     AGGGGRGMRLVRSPREMETSLPLARSEARAAFGDDSLYFERWIEDNRHVEVQVLIDTH
                     GNGIHLGERDCSVQRRHQKIIEEGPSPAMAEPARERLRDLAIRSVLSAGYENAGTLEF
                     LLDREGNFYFIEINCRIQVEHPVTEMLTGIDLVAEQIRIASGEPLTLTQEQVVIRGHA
                     VEFRITAEDPHDNFSPQSGTVSDLQLPGGAGVRVDTHLYPGYEIPPYYDSLLAKLVVW
                     GESRPEALARARRALAETHIEGVKTNLPFHRAIVDNAAFVDAEVSTSLLDRVGPAAFV
                     GEPASATIRQ"
     gene            1494..1934
                     /gene="fabZ"
                     /locus_tag="VGO32_08675"
     CDS             1494..1934
                     /gene="fabZ"
                     /locus_tag="VGO32_08675"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV7200861.1"
                     /translation="MTILPHRYPFLLIDRVIEIEPGKRLVAIRNVTANEPQFTGHFPD
                     RPIMPGVLLVESLAQAGAVAVLSYPENRGKLVLFAGIDECRFRRVVVPGDTLRLEITV
                     EKLGRVSGRARAVATVEGEVAVEALLSFILPRDQDIAGGRLSGG"
     gene            1927..3222
                     /gene="fabF"
                     /locus_tag="VGO32_08680"
     CDS             1927..3222
                     /gene="fabF"
                     /locus_tag="VGO32_08680"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019240485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV7200862.1"
                     /translation="MAEEAPRPSSRDRGERRVVITGLGAVTPLGNDVPSYWEGLVAGR
                     SGIARLASFDPSGVPSQVGGEIKDFDAAQFMDRKDVRRHDKYVHYAWAAAAQALADAG
                     LSNPIVDDADAERAGAIVATGIGGITSLVRDAQAALTEGYDRVGPFSILNMIPDTAAG
                     HVAIAANLRGPNYATVSACASSNHGIGEAAGIIRRGDADVMVAGGSEAAICEMVVAAF
                     CAMRALSTKRNDDPERASRPFDVDRDGFVIADGAGMIVLEDLEHAQARGATIRAEFIG
                     YGASDDASHVTMPAPGGRGAVASMRIALADAGLAPAELDYINAHGTSTPPNDPAETTA
                     IKTVFGEDAYRVPISSTKSMTGHLMGAGGGIEAVACVRALETGTLPPTINHDQADPDC
                     DLDYVPNAARQVPIATAMSNAFGFGGHNATLVFRRWDDA"
     gene            3226..3666
                     /locus_tag="VGO32_08685"
     CDS             3226..3666
                     /locus_tag="VGO32_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV7200863.1"
                     /translation="MADDEILAAVDALTQPFLDSGLDELEVESGGITVRLARPRPAAA
                     SAPGTSTSGAAGTGSPFGEPAPGHRFITAPLTGIWYGAPSPGARAYVQLNGEVAAGQV
                     VGLIEAMKLFNEIKSDVAGTVSRILADNGALVKRQQPLFEVRLA"
     gene            3663..4742
                     /locus_tag="VGO32_08690"
     CDS             3663..4742
                     /locus_tag="VGO32_08690"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV7200864.1"
                     /translation="MSATRAVVTGWGMYAPARIMTNDELSRIVDTSDEWIASRTGIRE
                     RRIAADDETTTTLATNAARDALAVAGVDPTEVDLVLVATASPDYLMPATGVLVARDLG
                     ATRAAGFDLNAACSGFLFGLAAGAAFVTSGMYRQVVVVGVDLLSRYLDWTDRNTCVLF
                     GDGAGAVLLSASDDGQGGLVSIRLFSDGTGEEGIIIPGGGTAHPATEETVRDRLHFMR
                     MAGKEVYRYATRQLAETTQAAVADAGLRMGDIDLFLYHQANIRILETVRDLLGIEAEK
                     VYVNIDRYGNTSAASVPMALAEAVATGRVQIGDRILMGAFGAGYTAGAAVLEWTADPT
                     RAFLAPGEQTRAGQPRREPVTAGSA"
     gene            4754..>5386
                     /locus_tag="VGO32_08695"
     CDS             4754..>5386
                     /locus_tag="VGO32_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7200865.1"
                     /translation="MFDLTGRSALVTGGSRGLGKAIALAFARQGADVAVNYRESAAGA
                     EAVVAEVTTLGRRAVAVQGSTADGREACEASVKAALDAFEKVDILVNNAGITRDDLIM
                     RMDADDWDSVIATNLSGPFWMTRAVSRPMMKARYGRIINMSSVAGRMGNPGQANYVAA
                     KAGLIGLTKTTARELASRNVTCNAIAPGLVETDILNTLSDAAREAIIQITP"
BASE COUNT          814 a         1934 c         1856 g          782 t
ORIGIN      
        1 tggtcgagct tgatcgcgcc gggggcggtt gatggtctcc aagatcctga tcgccaaccg
       61 gggcgagatc gccgttcgca tcctgcggac ctgtcgcgag atgggcattc cggcggtcgt
      121 cgccttcagc gagccggatc gcgagtccat ggccgtccgc caggcggacg aggccatctg
      181 catcggtcca gccgaggcac ggcgttcata cctgaaccag ccggccctga tcagcgccgc
      241 gctcatcacc ggctgcgatg ccatccatcc gggatacggc ttcctgtcgg aggacgcgtc
      301 gttcgcggag gcgtgcgcgg tccacgacct ggcgttcatc ggtccccggg cgcaggtcat
      361 cgagcggttt gcatcgaagt acgcggttcg ccgcatgctt gccgccaccg gtctgcccac
      421 cgtcccgggc agcacgggca tcgtggcgga tctggccgat gccctggccc aggccgagca
      481 ggctggctat ccggtgctgc tcaagccgtc cgccggcggc gggggtcgcg gcatgcgcct
      541 cgtccgctcg ccgcgcgaga tggagacctc attgcccctc gcgcgctccg aggcgcgcgc
      601 ggcctttggc gacgactcgc tgtacttcga gcggtggatc gaggacaacc gccacgtcga
      661 ggtccaggtg ctgatcgaca cccatggcaa cggcatccac ctgggcgaac gggactgctc
      721 cgtccagcgc cggcaccaga agatcatcga ggagggcccg tcgccggcca tggccgagcc
      781 tgcccgtgag cggctccgcg acctcgccat ccgctcggtg ctctcggccg ggtacgagaa
      841 cgcgggcacg ctcgagttcc tcctcgaccg ggagggcaac ttctacttca tcgagatcaa
      901 ctgccggatc caggtcgagc acccggtcac cgagatgctg accgggatcg acctcgtcgc
      961 cgagcagatc cggatcgcct cgggcgagcc gttgaccctc acccaggagc aggtcgtcat
     1021 ccgcggacac gcggtcgagt tccggatcac cgccgaggac ccgcacgaca acttctcgcc
     1081 acagagcggc acggtgagcg acctccagct gcctggtggc gccggcgtgc gggtcgatac
     1141 ccacttgtac ccgggctacg agattccgcc gtactacgac tcgctcctgg ccaagctcgt
     1201 ggtctggggc gaaagcaggc ccgaggccct ggcccgcgct cgtcgagcgc tggcggagac
     1261 ccacatcgag ggggtgaaga ccaacctccc cttccatcgg gcgatcgtcg acaatgcggc
     1321 cttcgtggac gccgaggtca gcaccagcct gctcgatcgc gtcggccccg ccgccttcgt
     1381 cggcgagccc gcgtcagcca cgatccgtca gtgagggacc gatgaccacc aaccccacga
     1441 ccgggggtcc ggacccggcc ctgccgccgc tcccgtggga ggcggcggac atcatgacca
     1501 tcctcccgca ccgctaccca ttcctgctca tcgaccgggt catcgagatc gagccgggca
     1561 agcggctggt cgctatccga aacgtgaccg ccaacgagcc gcagttcacc ggccacttcc
     1621 ccgatcggcc gatcatgccc ggcgtcctcc tcgtcgagtc gctggcccag gctggtgcgg
     1681 tggcggtcct gtcgtacccc gagaaccgcg gcaagctggt cctgttcgcc ggcatcgacg
     1741 agtgccgctt ccgccgggtg gtcgtcccgg gcgacaccct ccggttggag atcacggtcg
     1801 agaagctcgg ccgcgtgtcc ggccgtgcgc gcgcggtggc cacggtcgag ggcgaggtcg
     1861 cggtcgaggc cctgctctcg ttcatcctgc cccgcgacca ggacatcgcc ggtgggcggc
     1921 tgagcggtgg ctgaagaggc cccgcggccc agctcacgcg atcgcggcga gcgccgggtg
     1981 gtcatcaccg gcctgggcgc ggtcacgcca ctcggcaacg acgtgcccag ctactgggag
     2041 gggctggtgg ccggccggag cggcatcgca cgcctggcga gcttcgaccc gagcggggtt
     2101 ccctcccagg tcggcggcga gatcaaggac ttcgacgcgg cccagttcat ggaccgcaag
     2161 gacgtccgcc gtcacgacaa gtacgtccac tacgcgtggg ccgccgccgc ccaggcgctc
     2221 gccgatgccg gcctttcgaa cccgatcgtc gatgacgcgg acgcggagcg cgcgggggcg
     2281 atcgtggcca ccggcatcgg cgggatcacg tcgctcgtgc gcgacgccca ggccgccctg
     2341 accgagggct acgaccgggt cggcccgttc agcatcctga acatgattcc ggacacggcc
     2401 gccggccacg tggccattgc ggccaatctg cgcggcccga actacgcgac ggtcagcgcc
     2461 tgtgcgtcgt caaaccatgg gatcggggag gccgccggca tcatccgccg cggcgatgcc
     2521 gacgtgatgg tggccggcgg ctcggaggcg gcgatctgcg agatggtggt ggccgccttc
     2581 tgcgccatgc gcgcactgtc caccaagcgc aatgacgacc cggagcgcgc gtcgcgcccg
     2641 ttcgatgtgg atcgcgacgg cttcgtcatc gccgatgggg cggggatgat cgtgctcgag
     2701 gacctcgagc acgcccaggc ccgcggcgcg acgatccgag ccgagttcat cggctacggc
     2761 gcgtcagacg acgcctccca cgtgaccatg cccgcgccgg ggggtcgggg tgcggtggcc
     2821 agcatgcgga tcgcccttgc cgacgccggg ctggctcctg ccgagctgga ctacatcaac
     2881 gcccatggca cctccacccc gcccaacgac ccggccgaga cgacggcgat caagaccgtc
     2941 ttcggcgagg acgcctatcg ggtcccgatc agcagcacga agtcgatgac cggccacctg
     3001 atgggtgccg gcggcgggat cgaggcggtc gcctgcgtgc gggccctcga aacgggaacc
     3061 ctgcccccca ccatcaacca cgaccaggcc gatcccgact gcgacctcga ctacgtgccc
     3121 aacgcggccc gccaggtgcc gatcgcgacg gcgatgagca acgcgttcgg cttcggcggg
     3181 cacaacgcga ccctggtttt ccgccgttgg gacgacgcct gaccgatggc cgacgacgag
     3241 atcctggccg cggtcgatgc cctcacccag ccgttcctcg acagcgggct cgacgagctc
     3301 gaggtcgagt ccggagggat cacggtccgg ctcgcgcgtc cgcggccggc agccgcgtcc
     3361 gcaccgggca cgtcgacctc cggggccgcg gggacggggt ccccatttgg tgagcccgcc
     3421 cccggccatc ggttcatcac ggcgccgctg accggcatct ggtacggcgc gccgtcgccc
     3481 ggtgcccggg cgtatgtcca gctcaacggg gaggtggcag cgggacaggt ggtcggcctg
     3541 atcgaggcca tgaagctgtt caacgagatc aagtccgacg tggccggaac ggtgagccgc
     3601 atcctggccg acaatggggc cctcgtgaag cgccagcagc cgttgttcga ggtccgcctg
     3661 gcatgagcgc cacgcgcgcg gtcgtcaccg ggtgggggat gtacgcccca gcccggatca
     3721 tgaccaacga cgagctctcc aggatcgtcg acacctccga cgagtggatc gccagccgga
     3781 ccggcatccg cgagcggcgg attgccgcgg acgacgagac gaccaccacc ctggccacca
     3841 acgccgcacg tgacgcgctc gcggttgcgg gcgtggaccc gaccgaggtc gacctcgtcc
     3901 tggtcgccac cgccagcccc gactacctca tgccggccac cggtgtcctc gtcgcccgcg
     3961 acctgggtgc cacccgagcg gccgggttcg acctcaacgc ggcctgctca ggcttcctgt
     4021 tcggactggc cgccggcgcc gccttcgtga ccagcggcat gtaccgccag gtggtggtcg
     4081 tgggcgttga cctgctgagc cgctacctgg actggaccga tcggaacacg tgtgtcctct
     4141 ttggcgacgg tgcgggcgcc gtccttttgt ccgcgtccga cgacggtcag ggcggcctgg
     4201 tcagcatccg gctgttcagt gacgggaccg gggaggaggg aatcatcatc cccggcggcg
     4261 gcaccgccca tcccgcgacc gaggaaaccg tccgcgatcg actccacttc atgcggatgg
     4321 ccggcaagga ggtctatcgc tacgcgaccc ggcagctggc cgagaccacc caggccgcgg
     4381 tggcggacgc gggcctgcgg atgggcgaca tcgacctgtt cctgtaccac caggccaaca
     4441 tccggatcct ggagacggtg cgcgacctgc tcgggatcga ggccgagaag gtgtacgtga
     4501 acatcgatcg gtacggcaac acgtcggccg catcggtgcc catggccttg gccgaagcgg
     4561 tcgccaccgg ccgggtccag atcggtgacc gcatcctgat gggcgccttc ggcgcggggt
     4621 ataccgccgg tgccgcggtc cttgagtgga cggccgatcc gacccgcgcc ttcctcgctc
     4681 ccggcgagca gacccgcgcc ggccagcccc ggcgcgagcc ggtgacggcg ggcagcgcct
     4741 gacgatgggc ggcatgttcg acctcaccgg tcgctcggcc ttggtcaccg gcggcagccg
     4801 tgggctgggc aaggccatcg ccctcgcgtt tgcccgacag ggtgcggacg tggcggtgaa
     4861 ctaccgcgag agcgcggccg gcgccgaggc ggtcgtggcc gaggtcacga ccctgggacg
     4921 tcgggcggtg gcggtccagg gcagcacggc cgatgggcgt gaggcctgcg aggccagcgt
     4981 gaaggccgcg ctcgatgcgt tcgagaaggt cgacatcctg gtcaacaacg ccgggatcac
     5041 ccgcgacgac ctcatcatgc ggatggacgc cgatgattgg gactcggtca tcgcgaccaa
     5101 cctgtccggt cccttctgga tgacccgcgc cgtctcgcgg ccgatgatga aggcccgcta
     5161 cggccggatc atcaacatgt ccagcgtcgc cggccggatg gggaacccgg gccaggcgaa
     5221 ctacgtggcc gcgaaggccg ggctcatcgg tctgaccaag accaccgccc gcgagctggc
     5281 cagccggaac gtcacctgca acgcgatcgc acccggcctg gtcgagaccg acatcctgaa
     5341 caccttgtcc gacgccgccc gcgaagcgat catccagatc acgccc
//
LOCUS       DASYGU010000306         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_617048, whole genome shotgun sequence.
ACCESSION   DASYGU010000306 DASYGU010000000
VERSION     DASYGU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617048"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..592)
                     /locus_tag="VGO32_08700"
     CDS             complement(71..592)
                     /locus_tag="VGO32_08700"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEV7200866.1"
                     /translation="MTGASTAPFPSVGELNEVSVPTFVDAVAMLFERAPRFGAWLAAA
                     RPFTDDDDLMKAAARIAHQMTEPDQVELINAHPRLGSRDQLSDASRREQGPGADLVDA
                     ELAELNAEYEARFGLRYLVYVAGRPREALLPEFRAALERDRLPELRRALDDTLAIAAN
                     RLHFFRHTMGTPS"
     gene            complement(579..1034)
                     /locus_tag="VGO32_08705"
     CDS             complement(579..1034)
                     /locus_tag="VGO32_08705"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEV7200867.1"
                     /translation="MDVAEVGYPTQPIRADIQALAWVSGRWVGSHGADTIEEQWSAAA
                     GGGMMGMFRAITDSHPRFYELITFDPEGEGLVCRFKHFNRDLSGWEEKDEALTLDLVA
                     LTETDAVFLRRGAERWMTYRRAAADRLVVFFENAEDGHDPDDEYRFDRG"
     gene            complement(1041..1391)
                     /gene="uraH"
                     /locus_tag="VGO32_08710"
     CDS             complement(1041..1391)
                     /gene="uraH"
                     /locus_tag="VGO32_08710"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007727227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEV7200868.1"
                     /translation="MAAQRSARPTISTHVLDVETGAPAAGVSVVLFRRGDDDEFEVLN
                     EGETDADGRIADLVGGELTAGDYQLLFDLADYHDDDAFLQGLAVALRVTDPERSYHVP
                     LLLAPFSATVYRGS"
     gene            1462..1845
                     /locus_tag="VGO32_08715"
     CDS             1462..1845
                     /locus_tag="VGO32_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEV7200869.1"
                     /translation="MTSPDRFMIQRMGAEEGPFTLADLQQQVQAGFVKFDTPVRRADV
                     DGAAWFRAAEVPTLFSEKEWLVALLLSAFLGTLGIDRFYLGYTALGILKLITLGFCGI
                     WWIIDLILIATGNLQDAKGQVLRRS"
     gene            1842..2207
                     /locus_tag="VGO32_08720"
     CDS             1842..2207
                     /locus_tag="VGO32_08720"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEV7200870.1"
                     /translation="MSPRALRWLVAGALTMSVVVPTAFVRSGPRLCAFHAVTGRSCPS
                     CGMTRSWNAMGHGELRDAIGYHVLGPATFIAAAAVVAGGDGRAVRLLEPDTRMRPILA
                     ALAAVWVGAWARGLAAGEH"
     gene            2212..2628
                     /locus_tag="VGO32_08725"
     CDS             2212..2628
                     /locus_tag="VGO32_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEV7200871.1"
                     /translation="MSDLRITIGDFVFAARWEADAPRTRAALEPWLPIRAQLIHCKWS
                     GEGTWIPFGDQRPEVGYENHTSHPAPGQLIIYTGDLSECEILLAYGACDFSSKLGQLA
                     GNHFATIVSGAEQLPEMGRRVLWEGAKPIEITEGTA"
     gene            2625..>2845
                     /locus_tag="VGO32_08730"
     CDS             2625..>2845
                     /locus_tag="VGO32_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200872.1"
                     /translation="MTDLIVRGGTIVTAERSYEADVAVVDGRISAIGPDLSAEATMET
                     MDATGCLVLPGCVDVHTHTRLPTDDEPDR"
BASE COUNT          429 a          970 c          989 g          457 t
ORIGIN      
        1 agcggcgttc cgtaggtgcg gtagaccttg acctcggcct tcccgtactg gatgtcgtac
       61 tgaatatcgg tcatgaaggg gttcccatgg tgtggcggaa gaagtggagc cggttggcgg
      121 cgatggcgag cgtgtcgtcc agggctcgcc gcagctcggg gagccggtcc cgttcgagcg
      181 cggcccggaa ctcgggaagc agcgcctccc gcggccgacc tgccacgtac accaggtagc
      241 gtaagccgaa ccgcgcctcg tactcggcgt tgagctcggc aagctccgcg tccaccaggt
      301 ctgcgcctgg tccctgctcg cgccgggacg catccgacag ctggtccctc gagccaagtc
      361 ggggatgggc attgatgagc tcgacctggt ccggttcggt catctggtgc gcgatccgcg
      421 ccgcggcctt catgaggtcg tcgtcgtcgg tgaatgggcg ggccgccgcg agccacgcgc
      481 caaaccgtgg tgcccgctcg aacagcatgg cgacggcatc cacgaacgtg ggcaccgaca
      541 cctcgttcag ctccccgacg gacgggaacg gcgcggtgct agccccggtc aaagcggtac
      601 tcgtcatccg ggtcgtggcc atcctcggcg ttctcgaaga acacgaccag ccggtcggcg
      661 gctgctctgc gataggtcat ccagcgttcg gcgccgcggc gtaggaacac ggcatcggtt
      721 tcggtcaggg ccaccaggtc gagggtcagc gcctcgtcct tctcctccca cccggacagg
      781 tcgcggttga agtgcttgaa ccggcacacg agaccctcgc cctcggggtc gaaggtgatc
      841 agctcgtaga accgcgggtg actgtcggtg atcgcgcgga acatgcccat catcccgccg
      901 ccggccgctg cgctccactg ctcctcgatc gtgtccgccc cgtgcgagcc gacccagcgc
      961 ccgcttaccc aggccagcgc ctggatgtcg gcccggatgg gctgggtggg gtagccgact
     1021 tctgcgacgt ccatgcgcga tcagctgccg cgatagaccg ttgcgctgaa cggcgcgagg
     1081 agcagcggca cgtggtagct tcgctccgga tcagtgaccc ggagcgccac ggccagaccc
     1141 tgcaggaatg cgtcgtcatc gtggtaatcg gcgaggtcga agaggagctg gtagtcgcca
     1201 gcggtgagct cgccgcccac gaggtccgcg atgcggccgt cggcatcggt ctcgccttcg
     1261 ttcaggacct cgaactcgtc atcgtcgccg cggcggaaga ggacgacgga aacaccggcc
     1321 gcgggcgccc cggtctcgac gtcgagcacg tgggtcgaga tcgtgggacg agccgatcgc
     1381 tgggcggcca cggaacctcc tcatcaggcg ggggcggata tcctgtcagc cggatccgca
     1441 tgacccaccg ggagcatacc gatgaccagc cctgaccgat tcatgatcca gcgaatgggc
     1501 gccgaggaag ggcccttcac cctggcggac ctccagcagc aagtgcaggc cggattcgtg
     1561 aagttcgaca cgcccgttcg gcgcgcggac gtcgacgggg cggcctggtt ccgcgccgcg
     1621 gaggtgccga ccctgttctc cgagaaggag tggctcgtcg ccctgctcct gtccgcgttc
     1681 ctggggacgc tgggcatcga ccgcttctac ctgggctaca ccgcgctcgg catcctgaag
     1741 ctcatcacgc tcggattctg cggaatctgg tggatcatcg acctgatcct gatcgccacc
     1801 gggaacctgc aggacgccaa gggccaggtc ctgcgccgct cgtgagccct cgtgccctgc
     1861 gctggctcgt cgccggcgcc ctgacgatgt cggtggtcgt gcccacggcc ttcgtgcgga
     1921 gtggcccgcg cctgtgtgcc ttccatgcgg tgaccgggcg ctcctgcccg tcgtgcggga
     1981 tgacccgatc gtggaatgcg atgggccacg gcgagctgcg cgacgcgatc ggctaccacg
     2041 tcctcgggcc ggccaccttc atcgcggcgg ccgccgtggt ggccggtggc gacggacggg
     2101 cggtgcggct cctcgagccc gacacccgaa tgcgaccaat cctggcggcc ctggctgcgg
     2161 tgtgggtcgg tgcctgggca cgcggcctgg ccgcggggga gcactgaggg cgtgagcgac
     2221 ctccggatca ccatcggcga cttcgtgttc gcggcccgct gggaggcgga cgcgccacga
     2281 acgcgggctg cgctcgaacc atggctgccg attcgcgcgc agctcatcca ttgcaagtgg
     2341 agcggggaag gcacgtggat cccgttcggc gaccagcgac cggaggtggg ctacgagaac
     2401 cacacctcgc acccggcgcc gggccagctg atcatctaca ccggcgacct gtcggagtgc
     2461 gagatcctcc tcgcctacgg ggcgtgcgac ttctcgagca agctgggcca gctggcgggc
     2521 aaccacttcg cgaccatcgt ctccggcgcg gagcagcttc ccgagatggg acgccgcgtc
     2581 ctgtgggaag gggccaagcc gatcgagatc accgaaggga ccgcttgacc gatctcatcg
     2641 tccgcggcgg gacgatcgtg accgccgagc ggtcgtacga agccgacgtg gcggtggtcg
     2701 acgggcggat cagcgccatc ggtccggacc tctctgccga ggccaccatg gagacgatgg
     2761 atgcgaccgg gtgcctcgtc cttcctggct gcgtcgacgt ccatacccac acccgcctcc
     2821 cgaccgacga cgagccggat cgctt
//
LOCUS       DASYGU010000307         5720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1542449, whole genome shotgun sequence.
ACCESSION   DASYGU010000307 DASYGU010000000
VERSION     DASYGU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542449"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VGO32_08735"
     CDS             <1..647
                     /locus_tag="VGO32_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEV7200873.1"
                     /translation="ENVVHGDATVAFAVVRPPGHHAGKAQGKGFCLLNNVAIAVAALR
                     ADAGLERLAVLDWDVHHGDGSAELFAADPAVFYASTHQSPWYPGTGAMPEATDSMVTV
                     PLPAETGDEGFVDAWQQTVLPRLAAFRPEAIIMSAGFDAHRDDPLAWLEVSEQGFGEV
                     ARSVGEIARELGLTGVALALEGGYDLAAIRASAGATVRGLISGIKGSAEPGPRT"
     gene            665..1162
                     /locus_tag="VGO32_08740"
     CDS             665..1162
                     /locus_tag="VGO32_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV7200874.1"
                     /translation="MSSRRAPVAPADRPTRRSRPRRSSAAPAAPSTRPRKAAAAPPLA
                     VEIVTFGFKYGLPQKADLVFDVRFLTNPFWVPDLQPLSGLSAQVRQFVLDQPQAGRFL
                     DLVVQLLELTVPAYRAAGRDRLVVALGCTGGYHRSIALAEELAGRLGGLEGASVTVIH
                     RELRR"
     gene            1159..2475
                     /locus_tag="VGO32_08745"
     CDS             1159..2475
                     /locus_tag="VGO32_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEV7200875.1"
                     /translation="MTGRRFPRWLYPGMHIKRWLGAVLVGLTILALGAAILIIEFYRE
                     VILDIPQLALLLGADLEREIRGAIVITVGIFLTGFGMWKLMRSVVSPFVTTGDSVMEV
                     LYTKRYLARGPRIVALGGGTGLSTLLRGLKAYSANITAVVGVADDGGSSGRLRQQLGI
                     VPPGDIRNCIAALADAEPLMTQLMQYRFPPGSGLDDHAFGNLFIAAMTAVTGDFDEAV
                     RESNRVLAVRGQVLPATSVPLNLTARLASGRFISGQAAVSQATEPIARVQIDPSDVRA
                     TPEALERILEADLIVIGPGSLYSSVLPNLLISDIRDALVAAEGLRVYVCNVATQPGET
                     GGYTASQHLTTLFDHVGDQLVDMVLLNRNRSARRPTGWQGQPVQLDTRQLEELPVVVV
                     EDDIIDDQNAHHHDPDKLAAILLRLLQEDRPARQRQRIRRPVATAS"
     gene            2483..3289
                     /gene="whiA"
                     /locus_tag="VGO32_08750"
     CDS             2483..3289
                     /gene="whiA"
                     /locus_tag="VGO32_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV7200876.1"
                     /translation="MRPARDVKGELARIEPARECCREAEVAGLRFADDAIRTLDPSTA
                     RIAVRLGAGRAVPQSTDRGSGTRHHLALVSSSGRETWSWAKAAACDHRAFLRGVVLGS
                     GSLSFSRTGPHVEFVFRDAARARTLRRRLKASEIHASSYERRGRAVVYVKGRENVASL
                     LHLTGAHGALLELEAEQVGHDVQNRLNRLLNAEAANVNRTVHAAERQVRAIDRLEAEG
                     RLDELSPGLRLVADARRAHPEADLEALATELDLGRSAVHHRLRRIEQLAG"
     gene            3286..4050
                     /gene="tpiA"
                     /locus_tag="VGO32_08755"
     CDS             3286..4050
                     /gene="tpiA"
                     /locus_tag="VGO32_08755"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV7200877.1"
                     /translation="MTGPGRRPLVAGNWKMNPSDRDGARALARAVVEATQGAEAEIVL
                     CPPAIWLEDVAEAVRGSGIGVGAQTMHAQASGAFTGEISPPMVAGVAGYVILGHSERR
                     RLFGETDADVSEKVAAAVAHGLVPIAAVGESTEERRAGETEEVLTRQVRAAVSALPQV
                     AGSGLVVAYEPVWAIGTGDAASSADAQAGAALIRGLLDAGDPTGAAETRILYGGSVAP
                     ELAAGYFAEPDVDGALVGGASLDAAAFAAIVRAAVA"
     gene            4068..5612
                     /gene="gpmI"
                     /locus_tag="VGO32_08760"
     CDS             4068..5612
                     /gene="gpmI"
                     /locus_tag="VGO32_08760"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV7200878.1"
                     /translation="MAGTPSVVLCVLDGFGIGDDPSVDATNAANMPNWRRLMATWPHC
                     RLAAAGAAVGLPPGQMGNSEVGHLNLGAGFPVLQDLPRINRAIADGSFATNPALVAAS
                     RHARDRGGRLHLLGLIGPGGIHAIDDHLVAMAELAHDQGLPADRVLLHAFTDGRDTPP
                     RSAVTILPALLARLAGRATLATVTGRYWAMDRDGRWDRTQRAFEAIVRASGQTAASGE
                     EAVAVAYAAGVGDEFIEPTVVRPDGGVGAGDAIVHMNFRADRARQLTRALSRPDFSEF
                     DRGADMPQVPVTTLTEYQHAGNLPVAVAFGPIRADGLAAHLARLGRRQLHLAETEKYA
                     HVTYFFNGGIEEPFPGEERILVPSRRDVATYDKAPAMSAEAITDHLVAAITGGEYPFV
                     VVNYANPDMVGHTGDWDAALAALEVIDACLGRVADAATATGAALLITADHGNVERMRD
                     ESGGPHTAHTTADVPLVLVHRGHEAVGLRDGILADVAPTICALLEIPAGPAMTGHSLL
                     ASATTT"
BASE COUNT          783 a         2087 c         2005 g          845 t
ORIGIN      
        1 ccgaaaacgt ggtccatggc gatgccacgg ttgcctttgc ggtggttcgc ccgccgggcc
       61 atcacgcggg caaggcacag ggaaagggct tctgcctgct gaacaacgtg gccatcgcgg
      121 ttgcggccct gcgcgcggac gccggcctcg aacggcttgc ggtgctcgac tgggacgtcc
      181 atcacggcga cgggtcggcc gagctgttcg cggcagatcc ggccgtgttc tatgcatcaa
      241 cccatcagtc gccgtggtac ccgggcaccg gcgccatgcc ggaagccacc gactcgatgg
      301 tcacggtgcc cctcccggcc gagaccggcg acgagggctt cgtcgacgcc tggcagcaga
      361 ctgtgctccc gcgactggcg gcattccggc cagaggcgat catcatgtcg gccggcttcg
      421 acgcgcaccg cgacgacccg ctcgcgtggc tcgaggtcag cgagcagggc tttggcgagg
      481 tggcgcggtc ggtgggggaa atcgcccgcg agctcggtct cacgggggtg gctctcgcgc
      541 tggaaggtgg gtacgacctg gccgccattc gggcctcggc tggagccact gtccgcggtc
      601 tgatctccgg gatcaagggc tcggccgagc cgggtccacg aacctgaggg ctatactcga
      661 ttcggtgtcg tcccgacggg cccccgtggc tcccgcagat cgcccgaccc ggcgctcgcg
      721 accgcgccgc tcgtccgccg cgcctgccgc gccgtcgacg cggccccgca aggccgccgc
      781 ggctccgccg ctcgcggtcg agatcgtgac cttcggcttc aaatacggac tcccgcagaa
      841 ggctgatctc gtcttcgacg tccgattcct gaccaacccg ttctgggttc cggacctcca
      901 gcctctgtcg gggctctccg cccaggtccg ccagttcgtg ctcgatcagc cccaggccgg
      961 tcgcttcctc gatctcgtcg tccagctgct cgagctcacg gtcccggcct accgggccgc
     1021 cggccgggat cgcctggtcg tcgcgctcgg ctgcaccggc ggctatcacc gctcgattgc
     1081 cctcgccgaa gagctggccg gccggctggg cggcctcgag ggcgcatcgg tcaccgtgat
     1141 ccaccgggag ctgcgccgat gaccggccga cgattcccgc gctggctcta tcccggcatg
     1201 cacatcaagc gctggctggg cgcggtcctg gtcggcctca cgatcctcgc cctcggcgcg
     1261 gccatcctga tcatcgagtt ctaccgcgag gtcatcctcg atatcccgca gctggccctc
     1321 ctgctcgggg cggatctcga gcgcgagatc cggggcgcga tcgtgatcac ggtcggcatc
     1381 tttctcaccg ggttcgggat gtggaagctg atgcgcagcg tcgtgtcgcc cttcgtcacg
     1441 accggcgact cggtgatgga ggtcctctac accaagcgct acctggcccg cgggccgcgg
     1501 atcgtggccc tcggcggggg caccggcctg tcgaccctgc tccgcgggct gaaggcgtac
     1561 tcggccaata tcacggccgt ggtaggggtc gccgacgacg gcggcagcag cgggcggctt
     1621 cgccagcagc tggggatcgt gccgcccggc gacatccgga actgcatcgc ggccctggcc
     1681 gacgccgagc cgctcatgac ccagctcatg cagtaccgat tcccacccgg gtccggactc
     1741 gacgaccacg cgttcgggaa cctgttcatc gcggccatga ccgcggtgac cggcgacttc
     1801 gacgaggcgg tccgcgagtc gaaccgggta ctcgccgtgc gcgggcaggt gctgcccgcc
     1861 acgagcgtgc ccctcaacct caccgcgcgc ctcgcgtcgg ggcggttcat cagcggccag
     1921 gcggccgtca gccaggccac cgagccgatc gcccgggtcc agatcgatcc gagcgacgtg
     1981 cgagccactc ccgaggccct ggagcgcatc ctcgaggctg atctcatcgt gatcgggccc
     2041 ggcagcctgt actccagtgt cctcccgaac ctgctgatca gcgacatccg cgatgcgctg
     2101 gtcgcggcgg agggcctccg ggtctatgtc tgcaacgtgg ccacccagcc cggcgagacc
     2161 ggtggctaca ccgcctcgca gcacctgacc accctgttcg atcatgtcgg ggaccagctg
     2221 gtggacatgg tcctgctgaa tcggaaccga tcggctcgcc gtccgaccgg atggcagggt
     2281 caaccggtgc agctcgacac ccgtcagctc gaggagctgc ccgtcgtcgt ggtcgaggac
     2341 gacatcatcg acgatcagaa cgcccaccac catgaccccg acaagctggc cgcgatcctg
     2401 ctccgcctgc tccaggaaga ccgacccgcc cgccagcgcc agcgcatccg gcgtccggtg
     2461 gccacggcca gctgacgcgc gcatgaggcc cgcccgcgac gtgaaggggg agctggcccg
     2521 tatcgagccc gcccgcgagt gctgccgcga ggccgaggtc gccggactcc ggttcgccga
     2581 cgatgcgatc cgcaccctgg acccgtccac cgcgcggatc gcggtccggt tgggcgccgg
     2641 tcgggcggtt cctcagtcca ccgaccgggg atcggggacg cgtcaccacc tggcgctggt
     2701 ctcgagctcg gggcgagaga catggagctg ggccaaggcg gcggcctgcg accaccgtgc
     2761 cttcctccgg ggcgtggtcc tgggcagcgg ctcgctgtcg ttctcgcgga ccggaccgca
     2821 cgtcgagttt gtgtttcgcg acgcggcgcg ggcgcggacc ctccgtcgta gattgaaggc
     2881 gagcgagatc cacgcgtcca gctacgagcg ccgcggtcgg gccgtggtgt acgtcaaggg
     2941 ccgcgagaac gtcgcgtcgc tgctccatct gaccggcgcc cacggcgcgc tgctcgagct
     3001 cgaggcggag caggtgggcc acgacgtcca gaatcgcctc aaccgtctgc tgaacgccga
     3061 ggccgcgaac gtcaatcgga cggtgcacgc cgccgagcgc caggtccgcg ccatcgatcg
     3121 actcgaggca gaaggccgcc tggacgagct gtcacccggc ctgcggctgg tggccgatgc
     3181 gcggcgagcc caccccgagg ccgacctcga ggcgctggcg acggagctcg acctcggccg
     3241 ctcggccgtc caccaccggt tgcggcgaat cgagcagctc gccggatgac cggaccggga
     3301 cggcggccgc tggtcgcggg caactggaag atgaacccga gcgatcgcga tggcgcgaga
     3361 gccctcgccc gggccgtggt cgaggccacg cagggcgccg aggccgagat cgtcctctgc
     3421 ccgcccgcga tatggctcga ggacgtggcc gaagccgttc gcggtagtgg gatcggggtc
     3481 ggcgcccaga ccatgcacgc ccaggcgagc ggtgcgttca cgggcgagat ctcgccgccc
     3541 atggtggccg gcgtcgccgg ctacgtgatc ctgggtcact cggaacgccg gcgcctgttc
     3601 ggcgagaccg atgccgatgt ctcggaaaag gtcgcggcgg cggtggccca tggcctggtg
     3661 cccattgccg cggtcgggga gtcaaccgag gagcgccggg cgggggagac ggaggaggtc
     3721 ctgactcggc aggttcgggc cgccgtgtcc gcgctcccgc aggtggcggg cagcggcctc
     3781 gtcgtcgcct atgagccggt ctgggcgatc gggaccggcg acgcggcttc cagcgcggat
     3841 gcgcaggccg gcgcggcgct catccgcggc ctgctggatg ccggggaccc gaccggcgcg
     3901 gccgagaccc ggatcctgta tggcggcagc gtcgcacccg agctcgcggc cggctacttc
     3961 gccgagcccg acgttgatgg agccctcgtc ggcggtgcgt cgctcgatgc ggccgcgttc
     4021 gcggccatcg tccgcgcggc ggtcgcctga gccgccgcaa cccgaacgtg gcgggcactc
     4081 cctcggtcgt cctctgcgtg ctggacggtt tcgggatcgg ggacgacccc tcggtcgacg
     4141 cgaccaacgc cgcgaacatg ccgaactggc ggcggctgat ggcaacctgg ccccattgcc
     4201 gcctcgccgc tgccggcgcg gcggtcggtc tgccgccggg gcagatgggg aacagcgaag
     4261 ttggtcatct gaacctgggg gccggcttcc cggtcctcca ggacctaccg cggatcaacc
     4321 gcgcgatcgc cgatggatcc ttcgccacca atcccgcgct ggtcgcggcc agccggcacg
     4381 cgcgagaccg cggtggtcgg ctgcacctgc tcgggctcat cggtccggga ggcatccatg
     4441 ccatcgacga ccacctggtc gccatggcgg agcttgccca cgaccagggc ctgcccgccg
     4501 atcgggtgct ccttcacgca ttcaccgacg gccgggacac gcccccgcgc tccgcggtga
     4561 cgatcctgcc cgcgctcctc gcccgactcg ccggtcgcgc cacgctggcc accgtcaccg
     4621 ggcgctactg ggccatggac cgggacgggc gatgggaccg gacccagcgc gcgttcgagg
     4681 ccatcgtccg cgcgtcgggc cagacagcgg cctcgggcga ggaggccgtg gcggtggcgt
     4741 acgcggccgg cgtcggcgac gagttcatcg agcccaccgt ggttcgcccc gacggcgggg
     4801 tcggagccgg agacgcgatc gtgcacatga acttccgggc cgaccgcgcc cgccagctga
     4861 cccgcgctct gtcgcgaccg gacttctccg agttcgaccg cggcgcggac atgccgcagg
     4921 ttccggtcac caccctgacc gagtaccagc atgcgggcaa tctcccggtc gcggtggcct
     4981 tcgggccgat cagggccgac gggctggccg ctcatctcgc acgcctcggt cgccggcagc
     5041 tccacctggc cgagaccgag aagtacgccc acgtcacgta cttcttcaac ggcgggatcg
     5101 aggaaccgtt tcccggggag gagcggatcc tggtgccgtc gcgtcgggac gtggcgacct
     5161 acgacaaggc gccggccatg agcgcggagg cgatcaccga ccacctggtg gcggccatca
     5221 ccggaggtga gtacccgttc gtggtcgtga actacgcgaa tccggacatg gttggccaca
     5281 ccggcgactg ggatgccgcg ctggcggcgc tcgaggtcat cgacgcctgc ctgggccggg
     5341 ttgcggatgc cgcgacggcc accggcgccg ccctcctgat cacggccgac cacggcaacg
     5401 tggagcggat gcgagatgag tccggtggcc cgcataccgc ccacaccacg gccgatgtcc
     5461 cactcgtgct cgtccaccgc ggacacgagg ccgttggcct tcgggacggg atcctggccg
     5521 acgtcgcgcc caccatctgc gcgctgctcg agatcccggc cggcccggcg atgaccggcc
     5581 acagcctgct ggccagtgcc accacgacgt gaccttggct atactgccgc ccgcatgacc
     5641 gccctcaccc tcgtccagtc gatcctcgcc gtggccctga ccgtcgccat cctgcttcag
     5701 cagcgtggcg cgggggtcgg
//
LOCUS       DASYGU010000308         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_1388360, whole genome shotgun sequence.
ACCESSION   DASYGU010000308 DASYGU010000000
VERSION     DASYGU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388360"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1434
                     /locus_tag="VGO32_08765"
     CDS             169..1434
                     /locus_tag="VGO32_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEV7200879.1"
                     /translation="MTSGTPTSAPIALGPGNLTPDAVAALSASRTEPDWLHDARQAAV
                     ERYTATPWPTGAEEEWRRTSLRGLPVQSSLDEDAATEVSALDPDSVARGVVFASLADA
                     AREHPDLVRPLLSKPAEGPATHAAFWHLAHATWATGTFLYVPPNVTVDLVLRARTVAG
                     RADGAAFPVTYVIVGEGSEVTLLEEFSSPDGIPSWFGGIVDLRAERGARVRYASLQLL
                     GDQAWRVSAQRVIVGPDAVVTTLNAEIGSKVTKLGLDVRMTGTGGTSRLLGLLAAGDD
                     QKIDINSVQDLIGSHTTSDLLYLSALYDQAHAAFYGMTRVRSEAKQTSSYQECRNLLL
                     SPGAGAEPIPVLEIETNDILRCGHGATAGAIDPTQMFYAQTRGLTADEAERMIVRGFF
                     ERAVADIGSEPIRARVLAALASRIGQEAA"
     gene            1431..1775
                     /locus_tag="VGO32_08770"
     CDS             1431..1775
                     /locus_tag="VGO32_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7200880.1"
                     /translation="MSEAAEAMIPMGGFSTGLQATDVEPGTVVTVTVQGVPLCVGRTT
                     DGRWGAIDDVCTHDEGDLGDGELDGTAVECPRHGGRFDLFTGRVLALPPVIPVRAYPV
                     HVDDGAVMVDLP"
     gene            1772..2287
                     /locus_tag="VGO32_08775"
     CDS             1772..2287
                     /locus_tag="VGO32_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV7200881.1"
                     /translation="MKISTRSEYGIRVLLALARAEDDEPLSLTSVARTERLPHAYLEQ
                     LVADLRRAGLVTATRGQAGGYRLARPPQEISLATAIRTLDGPLLEMPCAGVESAERCD
                     RPEPCSVHEAYERLFESIDSTLGTTTLADLAAQAASAGGPPYSPDVRRRIRAAAEATA
                     HVQSQTMNGAP"
     gene            2284..>2696
                     /locus_tag="VGO32_08780"
     CDS             2284..>2696
                     /locus_tag="VGO32_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7200882.1"
                     /translation="MTDQLVISDLEVSAGKRKILKGLSLTVSQGEVHALMGPNGSGKT
                     SLAYALLGHPEYKVDAGDITWRGESLLKLSPDKRARAGMFLAFQYPSAIPGVTVASFL
                     RNIYNAVHHPVDPAAETNGAPPKYSGIPLAKFRKLL"
BASE COUNT          415 a          991 c          901 g          389 t
ORIGIN      
        1 ggcctgaccg aggccgaggc cagcgcgatg atcgtgaacg gattcatcga gcccatcgtc
       61 aaggagctgc cgatggagta cgcggtcgag atgaatcggc tcatccagct ccagatggag
      121 ggcgcggtcg gctagacccg cgggtccatt gccgatcctc ccccgccgat gacgtccggc
      181 acgcccacct ccgccccgat cgcgctcggc cccggcaacc tgaccccgga cgcggttgcg
      241 gcgctgtccg catcccgcac cgagcccgac tggcttcacg acgcacgcca ggccgccgtg
      301 gagcgctaca ccgccacgcc gtggcccacc ggcgcggagg aggagtggcg ccggacctcg
      361 ttgaggggac tgcccgtcca atcgtcgctg gacgaggacg ccgccaccga agtgtccgcc
      421 ctcgatcctg attcggtggc ccgtggcgtg gtgttcgcct ctctcgcgga cgccgcgcgc
      481 gagcaccccg acctcgtccg accgctgctc agcaagccgg cggaaggccc cgcgacccac
      541 gccgcgttct ggcacctggc ccatgcgacc tgggccacgg gcacattctt gtacgtcccg
      601 cccaatgtca cggtcgatct cgtgctccgg gcgcgcacgg tcgcagggcg agcggatgga
      661 gccgccttcc cggtcacgta cgtgatcgtg ggggaggggt cggaggtcac cctcctggag
      721 gagttcagct cacccgacgg catccccagc tggttcggcg gcatcgtcga cctccgcgcg
      781 gagcgcggag cgcgggtgcg ctacgccagc ctgcagctgc tcggcgacca ggcgtggcga
      841 gtatcggccc agcgcgtgat cgtgggccct gacgcggtcg tgacgacctt gaacgcggag
      901 atcggctcga aggtcaccaa gctcggcctg gacgtccgga tgacggggac cggcggcacg
      961 agccggttgc tcggcctcct tgcggcgggg gacgaccaga agatcgacat caactcggtc
     1021 caggacctca tcgggtccca tacgacctcg gatctcctgt acctgtccgc cctctacgac
     1081 caggcccatg ccgcgttcta cggcatgacc cgggtgcggt ccgaggcgaa gcagaccagc
     1141 tcgtaccagg agtgtcgcaa cctcctcctg tcgcccggcg cgggggccga accgatcccg
     1201 gttctcgaga tcgagaccaa cgacatcctg cgctgtgggc acggcgcgac cgccggcgcg
     1261 atcgacccga cccagatgtt ctacgcgcag acgcgtggcc tgaccgcgga cgaggcggag
     1321 cggatgatcg tgcgtggctt cttcgagcgc gcggtggccg atatcggctc ggagccgatc
     1381 cgggcgcggg tcctggccgc cctggcctcg cgcatcggtc aggaagcggc atgagcgaag
     1441 ccgcggaggc catgatcccg atgggtggat tctcgaccgg cctgcaggcc accgacgtcg
     1501 aaccggggac cgtggtgacg gtgacggttc agggcgtccc gctgtgtgtg ggccggacca
     1561 ccgatggccg atggggcgcg atcgacgacg tctgcaccca cgacgaggga gacctcggcg
     1621 acggcgagct ggacgggacc gcggttgaat gcccacgcca cggtggccgc ttcgacctgt
     1681 tcaccggccg cgtcctcgcc ctcccgccgg tcatcccggt ccgggcgtac cctgttcacg
     1741 tggacgatgg cgcggtgatg gtggacctgc catgaagatc agcacccgct cggagtacgg
     1801 catccgcgtc ctcctggcgc tggcccgcgc cgaggacgat gagccgctct cgctgaccag
     1861 cgtggcccgc accgagcgcc tgccccatgc ctatctcgag cagctcgtcg ccgacctgcg
     1921 ccgcgcaggc ctggtgaccg ccacccgggg ccaggccggc ggctaccggc tggcgcgacc
     1981 gccccaggag atcagcctgg cgaccgcgat ccgaacgctc gacgggcctc tgctcgagat
     2041 gccgtgcgcc ggtgtcgaaa gtgccgagcg ctgcgaccgc cccgagccgt gctcggtcca
     2101 cgaggcgtat gagcggctgt tcgaatcgat tgattccacc ctcgggacca ccaccctcgc
     2161 cgatctggcc gcgcaggccg catcggccgg tggaccgccc tactcccctg atgttcgacg
     2221 acgcatccgg gccgcggccg aggccaccgc ccacgtccag tcccagacca tgaacggagc
     2281 cccatgaccg accagctggt gatctctgac ctcgaggtca gcgccgggaa acgcaagatc
     2341 ctcaagggcc tgtccctgac cgtgtcccag ggcgaggtcc acgccctgat gggccccaac
     2401 ggctcaggca agacgagcct ggcctacgcc ctgctcggcc accccgaata caaggtggat
     2461 gccggcgaca tcacgtggcg tggggagagc ctcctcaagc tctcccccga caagcgggca
     2521 cgggccggaa tgttcctcgc cttccagtac ccgagcgcca ttccgggcgt gaccgtggcc
     2581 agcttcctgc ggaacatcta caacgccgtc caccacccgg tcgacccggc cgccgagacc
     2641 aacggtgcgc cgccgaagta cagcggcatc ccgctcgcca agttccgcaa gctcct
//
LOCUS       DASYGU010000309         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U4222
            k141_874095, whole genome shotgun sequence.
ACCESSION   DASYGU010000309 DASYGU010000000
VERSION     DASYGU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958319
            Sequence Read Archive: SRR11674120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:19:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,728
            Genes (coding)                    :: 1,716
            CDSs (with protein)               :: 1,716
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874095"
                     /isolate="SMAG_U4222"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VGO32_08785"
     CDS             complement(<1..295)
                     /locus_tag="VGO32_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7200883.1"
                     /translation="MTTDQTPAERPAPLIFMAAPSDPGDVTFGSYAAVLATGRHLLAG
                     ELSQRLARQGAAVQLIPPDRTPGAFHWGSWYTAAVQPALVAASGKVDAIGWVGA"
     gene            complement(292..900)
                     /locus_tag="VGO32_08790"
     CDS             complement(292..900)
                     /locus_tag="VGO32_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7200884.1"
                     /translation="MTSDTTARHTPDWVAELETGAPDVIAVWWLMDVDQGAARDELEA
                     RSGLGRAAAILAQQEVVADALEAILLAMPDEMLLEPGGEADWNVSQAFAHTTGARRYL
                     PAAAAMAANGRWPADDPPRVVPGIPGPADLDREALMGYLAKSRRSQALSASAISGHET
                     DPCPMDHPMIGHRISCGAWLLFAGVHDAMHLEQLHGLMDRAV"
     gene            complement(935..1264)
                     /locus_tag="VGO32_08795"
     CDS             complement(935..1264)
                     /locus_tag="VGO32_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV7200885.1"
                     /translation="MKLVTAIVHAEDAGALVDALLEKEYRTTRLNSSGGFLKQGNATI
                     IIGVDDDQVDAVLELISANCHARKQFVNPMPPIMEPGEFYMPYPVEVEVGGATVFVLP
                     VERFERL"
     gene            complement(1280..>1630)
                     /locus_tag="VGO32_08800"
     CDS             complement(1280..>1630)
                     /locus_tag="VGO32_08800"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV7200886.1"
                     /translation="VICARYLDSSLAYQGGGYGLDLDTLRTLQAFATGGLKPDLTILL
                     DVPVDIGLRRKRKDRWNRFEDTESAAFFERVRETYLQLAAAEPDRFRVLDGSGSVDET
                     DLAIRAVVAPLLDR"
BASE COUNT          239 a          564 c          585 g          242 t
ORIGIN      
        1 cggctccgac ccagccgatg gcgtcgacct taccgctcgc cgccacgagg gcgggctgga
       61 cggcggcggt gtaccacgag ccccagtgga acgcgccggg ggtccggtcc ggtggaatga
      121 gctggactgc cgctccctgt cgggcgagcc gctggctgag ctcccccgcg agcaggtggc
      181 gcccggtggc gaggaccgcg gcgtacgacc cgaacgtgac gtcgcccggg tcgctcgggg
      241 cggccatgaa gatcagcggc gcgggccgct cggccggcgt ctggtcggtg gtcacaccgc
      301 gcggtccatc aggccgtgga gctgctcgag gtgcatcgcg tcatggacgc cggcaaacag
      361 gagccacgcc ccgcagctga tccggtggcc gatcatcgga tggtccatcg ggcaggggtc
      421 ggtctcgtgg ccggagatcg cggaggcgga cagggcctgt gaacgacggc tcttggccag
      481 gtagcccatc agcgcctcgc ggtccaggtc cgccggcccg ggaatccccg gcaccacccg
      541 tggcgggtcg tccgccggcc agcgcccatt ggcggccatg gccgcggcgg cggggaggta
      601 gcgacgggcg ccggtggtgt gggcgaacgc ctgcgacacg ttccagtcgg cctcaccgcc
      661 cggctcgagg agcatctcgt ccggcatggc cagcaggatc gcctccagcg cgtcggcgac
      721 cacctcctgc tgggcgagga tcgcggcggc acggccgagg ccggaccgcg cttcgagctc
      781 gtcccgggcg gcgccctggt cgacgtccat cagccaccag accgcaatca cgtccggggc
      841 gcccgtctcg agctcggcca cccagtcagg ggtgtgccgg gcggtggtgt cggaggtcat
      901 cggaccgatc ctagccctgt cgcccccggg gcggttacag gcgctcgaag cgctcgacgg
      961 gaaggacgaa cacggtggcc ccgccgacct ccacctcgac cgggtagggc atgtagaact
     1021 cgcccggctc catgatcggc ggcatcgggt tcacgaactg ctttcgcgcg tggcagttgg
     1081 cgctgatcag ctcgagcacg gcatccacct ggtcgtcatc gacgccgatg atgatcgtcg
     1141 cgtttccctg cttcagaaac ccgccggacg agttcagccg agtggtccgg tattccttct
     1201 cgaggagggc atccaccagg gctccggcgt cctcggcatg gacgatggcg gtgaccagct
     1261 tcatcggttt cagtatgccc taccgatcca ggagcggagc caccaccgcg cggatggcga
     1321 ggtcggtttc atcgaccgat ccggacccgt ccagcacccg gaaccgatcc ggctcggcgg
     1381 cggccagctg aagatacgtt tcgcggaccc gttcgaagaa cgcggcactc tcggtgtcct
     1441 cgaatcggtt ccatcggtcc ttccgcttgc ggcgcagacc gatgtcgacc gggacgtcga
     1501 gcaggatcgt gaggtcgggc ttcaacccgc cggtggcgaa cgcctggagc gtccgaagcg
     1561 tgtccaggtc gagcccgtag ccgccgccct ggtacgcgag cgacgagtcg agataccggg
     1621 cgcagatcac
//
